LOCUS       DAWTQS010000001       108356 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.001, whole genome shotgun sequence.
ACCESSION   DAWTQS010000001 DAWTQS010000000
VERSION     DAWTQS010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 108356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 108356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..108356
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.001"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            125..374
                     /locus_tag="WNA25_000001"
                     /pseudo
     CDS             125..374
                     /locus_tag="WNA25_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687885.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1251..1522
                     /locus_tag="WNA25_000002"
                     /pseudo
     CDS             1251..1522
                     /locus_tag="WNA25_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220648.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1758..2044
                     /locus_tag="WNA25_000003"
                     /pseudo
     CDS             1758..2044
                     /locus_tag="WNA25_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217501.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2469..2693
                     /locus_tag="WNA25_000004"
     CDS             2469..2693
                     /locus_tag="WNA25_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9822884.1"
                     /translation="MMKNKYILIKAAIGITAISIFYLLTQGSIGKTEEPSYFLMFMFL
                     NSLWFEENKTVMAAVTAMIAAHFIFVALSD"
     gene            2749..3405
                     /locus_tag="WNA25_000005"
     CDS             2749..3405
                     /locus_tag="WNA25_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFA9822885.1"
                     /translation="MEQKRRFAASLLLAAALPLCAHSFPFAEENPIAYGKVKIQSWKA
                     RRDFNIVKQDLDFSCGAASVATLLNNFYGQKLTEEEVLEKLGKEQMRASFEDMRRIMP
                     DLGFEAKGYALSFEQLAQLKIPVIVYLKYRKDDHFSVLRGVDGNTVLLADPSPGHVSM
                     SRAQFLEAWQTREGNLAGKILAVVPKKAEAISNKLFFTHHPKRQTEFAVGQIRQGRAE
                     "
     gene            3533..4828
                     /gene="hisS"
                     /locus_tag="WNA25_000006"
     CDS             3533..4828
                     /gene="hisS"
                     /locus_tag="WNA25_000006"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFA9822886.1"
                     /translation="MAQKIQSVKGMNDLLPVGQKDFKLTAVFWQAFEDTVNRWTRAYG
                     YQQIRTPIVEQTGLFVRSIGEETDVVGKEMYTFSDSNDSLSLSLRPEGTASCLRAVVE
                     HNLLYNSPQKLWYMGPMFRRERPQKGRYRQFHQVGIEALGFEGPDIDAEIIAMSADLW
                     EKLGIREYLTLEINSLGNREERAAHRAALVEYLTRYEAQLDEDSKRRLKTNPLRVLDT
                     KNPDLQEICNAAPRLVDYLGEASQNHYARFKAMLDGLGIQYIENSRLVRGLDYYNQTV
                     FEWTTDKLGAQATVCGGGRYDGLIEELGGKPAPSIGFAMGIERLLLLVSEYGSLEVNA
                     APDVYAMHQGEGADLQVMKYAQALRAQGFNVMQHSGYQSLKAQMKKADNSGARFALIV
                     AQDELADGTVTLKDMNGAHGQQTVSATDLTDTLQQWKNA"
     gene            4829..5458
                     /locus_tag="WNA25_000007"
     CDS             4829..5458
                     /locus_tag="WNA25_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HFA9822887.1"
                     /translation="MAAHLEEQQELDNFKYFWKTTGKWLFALLILAALGYLGYTVYQN
                     RAASQNQEAAAVLANIVEKAQNKAPQSEINAELSKLQQSYPHSISAAQATLMAAATEF
                     DAQRYDVAEGHLKWVLSNQKDSLIQALAAQRLGVVLLQQKKYDAALAALDTPVEADFA
                     PLLMETKGDVYAAQEKSQEALKNYGQALEKMPQDSVGRELLQMKLDSLK"
     gene            5608..7065
                     /gene="der"
                     /locus_tag="WNA25_000008"
     CDS             5608..7065
                     /gene="der"
                     /locus_tag="WNA25_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFA9822888.1"
                     /translation="MKPTIALIGRPNVGKSTLFNRLTRTKDALVHDLPGLTRDRHYGH
                     GKVGSKPYFVIDTGGFEPVVDSGILHEMAKQTLQAVDEADAVVFLVDGRTGLTPQDKI
                     IADRLRQSPRPVYLAVNKGEGGDRAVLAAEFYELALGEPHVISGAHGDGVYYLIEEIL
                     ENFPEPEAEEADAKHPVFAVIGRPNVGKSTLVNAILGEKRVIAFDMAGTTRDSIHIDF
                     EREGKPFTIIDTAGVRRRGKVDEAVEKFSVIKAMQAVEAANVAVLVLDAQQDIADQDA
                     TIAGFALEAGRALVVAVNKWDGISEERREQVKRDISRKLYFLDFAKFHFISALKERGI
                     DGLFESIQAAYNAAMIKMPTPKITRVLQTAVGRQQPPRAGLVRPKMRYAHQGGMNPPV
                     IVVHGNSLHAISDSYTRYLTQTFRKAFNLQGTPLRIQYNVSENPYENAEDKPKKKPLR
                     RVSLSNRIEKREGRKEEKNRFKKKTKVSVKKQFSK"
     gene            7096..7995
                     /gene="rdgC"
                     /locus_tag="WNA25_000009"
     CDS             7096..7995
                     /gene="rdgC"
                     /locus_tag="WNA25_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFA9822889.1"
                     /translation="MWFKQISFYPLNKEKLPEADVLADKLAEAEFTHCQGLDWFSEGF
                     TAPVSFSPELVFPADFTLRVALKKEEKVLPAGVIRDILEEKVAEIQNNEARNVGRKEK
                     QELKEQITDDLLPRAFTRSSRTEAVFNTRHGYLLVNNAASAKAENILTKLREALGGLE
                     ASLPNTKQSPSSLMTGWLLQGHCEGGFELDSDCELKGTGDIVPVVKVSKQDLTADEVV
                     QHVKNGKTVTRLGLVWREQIAFILTQDFTLKRIQYLDVLQEEAESNGDDAAGLAFASQ
                     ILMAESVSTMLEELVSYLGGWQD"
     gene            8047..8436
                     /locus_tag="WNA25_000010"
     CDS             8047..8436
                     /locus_tag="WNA25_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9822890.1"
                     /translation="MLKHLAFLLPAMMFALPAQTAVLSPYQETGCTYEGGIGKDGLPS
                     GKGIWRCRDGRGYTGSFKNGKFDGQGVYTVAAGREVFLEPFNSDSTKFRNMALSGTFK
                     QGLAHGRFAASQNGETLFYYEMRTRHD"
     gene            8531..9097
                     /locus_tag="WNA25_000011"
     CDS             8531..9097
                     /locus_tag="WNA25_000011"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFA9822891.1"
                     /translation="MSIKSDKWIRRMSEEFGMIDPFEPNQIKEADGQRIISYGTSSYG
                     YDIRCANEFKIFTNINSTIVDPKNFDPKNFVTVEDDCCIIPPNSFALARTVEYFRIPR
                     NVLTVCLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYAGEGVAQVLFF
                     ESDEVCETSYKDRNGKYMGQTGVTLPKA"
     gene            complement(9163..9867)
                     /locus_tag="WNA25_000012"
     CDS             complement(9163..9867)
                     /locus_tag="WNA25_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248833.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HFA9822892.1"
                     /translation="MLRSVLAASLLAVSFPAAAEALNYNIVEFSESAGIEVAQDTMSA
                     RFQVAAEGRDKNAVNAEFVKKFNNFTRKSKNGSFKTELVSRSAMPRYQYTNGRRIQTG
                     WEERAEFKAEGRDFDALNRFIADVQTDASLEDTDFSVSRERRNEVIDQVSKDAVLRFK
                     ARAEKLAGVLGASGYKIVKLNFGQIGSHIAGDGAVRAKMLRAMPMAASVNMKGTDSAA
                     PGVEEISISINGTVQF"
     gene            10034..10621
                     /locus_tag="WNA25_000013"
     CDS             10034..10621
                     /locus_tag="WNA25_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFA9822893.1"
                     /translation="MVSTIRTGCGRNIFPKSSTGPPTQRHQNRHPRPHVPLSAERNSK
                     HPDRSLRFCPIPAKRFGHLRMAQHVFLPRFPFIDIDRIRWVRAGLKAFKHYIRENGLP
                     DLIHAHCMNYAGILAQKISEKYGIPYVLTEHSSTITRGLIRHHQWQPMEKAAAPASAR
                     LAVSRHFAHVLQHKYGCEWQYLPNIPGGIFKQTFE"
     gene            10649..11206
                     /locus_tag="WNA25_000014"
     CDS             10649..11206
                     /locus_tag="WNA25_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFA9822894.1"
                     /translation="MFCTVSHLRRLKGHDVLLTAFARALAQCPQLRLNIGGSGQEEQR
                     LKQQAADLGIAHAVTFLGALQPEAILDLMRNSDAFILASRTETFGVVYIEALSQGLPV
                     IAIHCGGAESIVSDGNGYLVSVDDADVLAFLKIYEHHADFSAEQLRTDCLATFGEDAV
                     IGRLIAVFRQAARNTARKRPKNRLK"
     gene            11232..12185
                     /locus_tag="WNA25_000015"
     CDS             11232..12185
                     /locus_tag="WNA25_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFA9822895.1"
                     /translation="MTHTVHLHLEETDNPTLQRLCGSFDSNLDSLAKALDIHISRRFE
                     HFTFNGAFAHAGKRALLKLLETAQTRDLNDSIIRLAAVETQTEDAGHQEKNHEHAYYF
                     RTKRGSIGGRTPRQNGYIRALLDHDIVFGLGPAGTGKTYLAVAAAVDAMEKHQVERII
                     LVRPAVEAGEKLGFLPGDLTQKVDPYLRPLYDALYDLMGFDRVTKLIEKGLIEITPLA
                     YMRGRTLNGAYIILDEAQNTTPEQMKMFLTRIGFGAKAVITGDTSQIDLPKNIKSGLK
                     DAREKLHGVAGLYFHTFTGEDVVRHPLVQKIVEAYESAEHD"
     gene            complement(12290..12613)
                     /locus_tag="WNA25_000016"
     CDS             complement(12290..12613)
                     /locus_tag="WNA25_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9822896.1"
                     /translation="MKKCILGILTACAAMPAFADRISDLEARLAQLEHRVAVLESGGN
                     TVKIDLFGSNSTMYVCSVTPFQKTFEASDRNEGVARQKVRQACNRETSAMFCGDEAIR
                     CRKFD"
     gene            complement(12786..14144)
                     /locus_tag="WNA25_000017"
     CDS             complement(12786..14144)
                     /locus_tag="WNA25_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFA9822897.1"
                     /translation="MLKKWLNKMLPSGRSSKKAESKTVIPAERHNISAEMLSFAAENV
                     IRRLKGAGFQAYVVGGAVRDLLLGIEPKDFDVATDATPEQVHKLFRRSRIIGRRFQIV
                     HVMNGAEIIEVTTFRGGAKVHQNAHGRIMKDNTYGSIEEDAMRRDFTCNALYYDPEKE
                     EILDFHNGIADVAARRLVMIGNAAERYQEDPVRILRAIRLSGKLGFELSEETAAPIAE
                     SICRLKHEPVARLFDEIMKLLFSGHARECLKRLNGFDIPDIHPLLNALRVSDGIAGKM
                     TALALKNTDERLRADKSVSVGFVLAALMWPELDRHWKSNLQQGLKPTPALSDAINTMR
                     ETVERGWGVPQRFSATMREIWMFQPQFENRKGARPHKLFAQARFRAAYDFLLLRAETG
                     NADRALAEWWTAFQTASAEQRTEMTKNEAAARHEKNEGQAKKRRRRRRKPKPKVVGTD
                     WE"
     gene            complement(14502..16202)
                     /gene="recJ"
                     /locus_tag="WNA25_000018"
     CDS             complement(14502..16202)
                     /gene="recJ"
                     /locus_tag="WNA25_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFA9822898.1"
                     /translation="MSVKIQTRPVNTDVFNHLLAAGADPLIARLCASRGVQSPAELDD
                     KLASLLPYQSLTNCEAAAGRLADAVERKEKILIVADYDADGATACAVGLDGLAAMGAK
                     VDFLVPNRFEHGYGLTPELAEIAAAQGVDLLITVDNGIASIAGVARAQALGLDVIVTD
                     HHLPADTVPDCIIVNPNQKGCGFPSKSLAGVGVIFYVLTALRAELRRRNYFSDGIKEP
                     NLGGLLDLVALGTVADVVPLDHNNRILVSQGLKRMRSGKMRPGIRALFEVARRDWRKA
                     QPFDMGFALGPRINAAGRLDDMSVGIACLLARDDSEAQELAARLNNLNIERREIEQSM
                     LRDALNAFPETLPSGQTTLVAYRDDFHQGVVGIVASRLKDRFYRPTIVFAPADNGEVR
                     GSGRSIPNLHLRDALDLVSKRHPDLILKFGGHAMAAGLSILEHNIPAFQTAFEEAVRE
                     MVCEDDLSQTYITDGSLPACDITLEQAQNLACHVWGQGFAPPSFTDEFHVVRQQPLGA
                     EGKHKKAWLQKDGCEFEAMFWRCSEDIPEYIRTVYRPVANEWRDNLELQLYIDYWEAA
                     "
     gene            complement(16271..16984)
                     /locus_tag="WNA25_000019"
     CDS             complement(16271..16984)
                     /locus_tag="WNA25_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9822899.1"
                     /translation="MLFRKTTAAVLAATLILNGCTMMLRGMNNPVSQTITRKHVDKDQ
                     IRAFGVVAEDNAQLEKGSLVMMGGKYWFAVNPEDSAKLTGLLKAGLDKPFQIVEDTPS
                     YARHQALPVKFEAPGSQNFSTGGLCLRYDTGRPDDIAKLKQLEFKAVKLDNRTIYTRC
                     VSAKGKYYATPQKLNADYHFEQSVPADIYYTVTEKHTDKSKLFGNILYTPPLLILDAA
                     AAVLVLPMALIAAANSSDK"
     gene            complement(17031..17621)
                     /locus_tag="WNA25_000020"
     CDS             complement(17031..17621)
                     /locus_tag="WNA25_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HFA9822900.1"
                     /translation="MQGVRCKKPSERGGILLKYAIGKNSRIKMFEQEDEWISKTQMKK
                     QMNGLQDLGMELTRLSNDTLKKIGLDEDLYEAVVTYKKITSNGALKRQAQFIGRLMRD
                     TDPAPIEAFLAKLRGDDAAHNAFLQRVEQARVRLLADEGALTQFMSDFPNADAGRLRT
                     LVRNTKKEQEQDKPPKNFRALFQELKTVMENGGAEI"
     gene            17691..19022
                     /gene="pmbA"
                     /locus_tag="WNA25_000021"
     CDS             17691..19022
                     /gene="pmbA"
                     /locus_tag="WNA25_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFA9822901.1"
                     /translation="MLFNHTASELLDLCRRTLDLAKAAGATAAEADFSESLGQSVSVR
                     LGEIEQIEFQQDKSLDITVYVGKRKGRAGTADFSEQALRDTVRAAADIARHTAEDGCA
                     GLADPELMAQHIGDPDLYHEWDLDTEAAVGLAKQCEQAALDTDSRIENSEGAAVQTGH
                     YQYVYGNTHGFAAHRQSTHHSISCSVVAADENGMQRDYWYDSACRHPDMDSPETIGQT
                     AARRTLRRLGSRSIPTGSYPVLFDTTVSGGLIGHLVGTLSGGALYRQSSFLIDSIGKK
                     VLPDFLNLREEPHIPRSFRSSYFDAEGVATAPRFVIQNGIVEGYFLGSYSARKLGMQT
                     TGNAGGAHNLYLNHTHETQSDLLKEMGTGLLVTELMGQGANTITGDYSRGAAGFWVEN
                     GVIAYPVHEITVAGRLQDMYRDIVGVADDALRRSSNKIGSILIAGMTVAGS"
     gene            complement(19321..19524)
                     /locus_tag="WNA25_000022"
     CDS             complement(19321..19524)
                     /locus_tag="WNA25_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFA9822902.1"
                     /translation="MATGIVKWFNDAKGFGFITPDEGGEDLFAHFSAINMEGFKTLKE
                     GQRVSFDVTTGPKGKQAANIQAA"
     gene            19769..20071
                     /gene="clpS"
                     /locus_tag="WNA25_000023"
     CDS             19769..20071
                     /gene="clpS"
                     /locus_tag="WNA25_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFA9822903.1"
                     /translation="MTAQHQSDTLLHRLNTLPPKRYGVFLLNDDYTTMEFVVEILTEI
                     FMLGQEQAVAVMLSVHHEGKGLCGTYTRDIAQTKQQQVMQRAKAEGHPLQCIVEEI"
     gene            20073..22364
                     /gene="clpA"
                     /locus_tag="WNA25_000024"
     CDS             20073..22364
                     /gene="clpA"
                     /locus_tag="WNA25_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFA9822904.1"
                     /translation="MLAPELEQILQQLYREARKAHYEFISLEHLLLVLIEEDAAVPNV
                     LKLCGADLKAVSEQLAASVAENTPLIPDHLLDTVETRPTLGFQRVIQRAMVHTQSAGK
                     GLAEPLDVLVALMSETDSHAVYFLKLQSVTRFEVLRCIAHGSPDEDEDDGNYFSDGMD
                     DDNENRTKPGKNPLSAYTVNLNAEVKAGRIDPLIGRKHEMERLVQILCRRRKNNPLLV
                     GEAGVGKTALADGLAHQIVNDDIPDALKEAEVYALDMGSLLAGTKYRGDFEARVKSVL
                     KQLEKIPHAILFIDEIHTIIGAGSTGGGTMDASNLLKPALAKGSLRCIGATTYDEYRT
                     IFDKDHALSRRFQKIDVVEPTVAETVQILRGLKPMFEAFHQVRYTQGALEAAAELSAR
                     YINGRFLPDKAIDVMDEAGAAQRILPKSKQKKVIGKAQIETVIAKVARIPEKTVSHDD
                     KQVLQFLGRDLNNMVYGQEDAIDALVSAVKMSRSGLGLPDKPIGSFLFSGPTGVGKTE
                     VAKQLAYSMGVPLQRFDMSEYMERHAVSRLIGAPPGYVGFEQGGLLTEAVNKQPHCVL
                     LLDEIEKAHPDIFNVLLQVMDAGKLTDNNGKSADFRNVILIMTTNAGAESLSRPSLGF
                     TAKRERGDEMQAINKLFTPEFRNRLDAIIPFAPLSEPIIVKVVDKFLLQLEHRLLDKK
                     VEAEFTPALRKYLAEKGFDPQMGARPMHRLIQEKIRKPLADELLFGKLADGGFVRIDW
                     DAAKEEAVLKFKKSKVKIKTASA"
     gene            complement(22404..25502)
                     /locus_tag="WNA25_000025"
     CDS             complement(22404..25502)
                     /locus_tag="WNA25_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFA9822905.1"
                     /translation="MNLETKPIAETPNFIVLDQYEKIEQSGSYQSENRLEAELIADLQ
                     NQGYEYRKDLNSQSRLLENLRAQLQRLNDVAFSDGEWARFLTEYLDRPAENITDKTRK
                     IHDDHIYDFAFDDGRLKNIYLLDKKNLARNHVQLINQFEQTGTHANRYDVTVLVNGLP
                     LVQIELKKRGVAVREAFNQVHRYSKESFNSENSLFKFLQIFVISNGTDTRYFANTTKR
                     DKNSFDFTMNWARSDNHPIKDLKDFTATFLQKSVLLSVLLHYSVFDANDTLLIMRPYQ
                     IAAAERILWKINSSAQAKNWSGPESGGYVWHTTGSGKTLTGFKAARLATESAFIDKVF
                     FVVDRKDLDYQTMKEYQRFSPDSVNGSESTAGLKRNLEKDGNKIIVTTIQKLNNLMKS
                     EDNLPVYHRQVVFIFDECHRSQFGEAQKNLKKKFKKFCQFGFTGTPIFPENALGAETT
                     AGVFGRELHSYVITDAIRDEKVLKFKVDYNDVRPQFKAVEAEQDEKELSAAENRQALL
                     HPERIREITQYILSRFRQKTHRLNAGGKGFNAMFAVSSVDAAKCYYEAFKTQQAGSLH
                     PLKTATIFSFAANEEQNAVGEIVDETFEPEAMDSSAKEFLQAAINDYNACFKTNFGTD
                     SKAFQNYYRDLAKRVKNREVDLLIVVGMFLTGFDAPTLNTLFVDKNLRYHGLMQAFSR
                     TNRIYDATKTFGNIVCFRDLEQATIDAITLFGDKNTKNIVLEKSYEEYMNGYTDSQTG
                     EARRGYLDVAKELHERFPDPDKIETEKDKKDFAKLFGEYLRAENVLQNYDEFAALREL
                     QNVDAADEDAMKAFQEKYYLSDEDVQEMRKVPMPSERAVQDYRSAYNDIRDWLRRQKA
                     GEQKEQSKIDWDDVVFEVDLLKSQEINLDYILQLVFEHHKKIKGKAELVEEIRRIIRA
                     SIGHRAKEGLIVDFINDTDLDKVPDVPAILETFYTYAQEVMRHEAAGLIAAEGLNETA
                     AKRYLTGSLKRGYASENGTELTETLPKMSPLNPQYLTKKQSVFQKIAAFVEKFAGIGA
                     DI"
     gene            complement(25734..26957)
                     /locus_tag="WNA25_000026"
     CDS             complement(25734..26957)
                     /locus_tag="WNA25_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFA9822906.1"
                     /translation="MDMQSKAKKLIEMIQTAPVEWKPLGEVLVRTKGTKITAGQMKEM
                     HKDNAPLKIFAGGKTFALVDFDDVPDKDIHREPSIIVKSRGIIEFEYYDKPFSHKNEM
                     WSYHSVNKHIYIKYVYYFLKTQENYFRNIGSKMQMPQIATPDTDNYKIPIPSLETQQK
                     IVKILDKFTELEATLEATLEAELALRKRQYRYYRDLLLDFDNQIGGGIADGYQCRLKN
                     VVWKTLGEVAEYSKNRICSDKLNEHNYVGVDNLLQNREGKKLSGYVPSEGKMTEYIVN
                     DILIGNIRPYLKKIWQADCTGGTNGDVLVIRVTDEKVNPKYLYQVLADDKFFAFNMKH
                     AKGAKMPRGSKAAIMQYKIPIPPLPEQEKIVAILGKFDTLTHSVSEGLPHEIALRRKQ
                     YEYYREQLLAFPKAA"
     gene            complement(27124..27975)
                     /gene="dinD"
                     /locus_tag="WNA25_000027"
     CDS             complement(27124..27975)
                     /gene="dinD"
                     /locus_tag="WNA25_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFA9822907.1"
                     /translation="MTTENNAFENAKHIDETGNEYWSARTLQQILEYSEWRNFQRAID
                     KAITACETSGNDKNHHFVETNKMIALGKGGQREVADYRLSRYACYLIVQNGDPSKSVI
                     AAGQTYFAVQARRQELQDEAAFRSLGEDKQRLLLRRQLREHNTDLAAAAKDAGVEKPV
                     EYAVFQNHGYRGLYGGLDKQGIHSRKGLKKSQRILDHMNASEPAANLFRATQTEEKLR
                     RKNIQGKTQANRVHFEVGQKVRQTIEELGGIMPENQPVPEKSIKQLENEEQKRLAATE
                     QHQNGKK"
     gene            complement(28091..29635)
                     /locus_tag="WNA25_000028"
     CDS             complement(28091..29635)
                     /locus_tag="WNA25_000028"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFA9822908.1"
                     /translation="MMTEMQQRAQLHRQIWKIADEVRGAVDGWDFKQYVLGTLFYRFI
                     SENFTDYMQAGDSSIDYAAMPDSIITPEIKDDAVKVKGYFIYPGQLFCNIAAEAHQNE
                     ELNTKLKEIFTAIESSASGYPSEQGIKGLFDDFDTTSSRLGSTVADKNKRLAAVLKGV
                     AELDFGNFEDHRIDLFGDAYEYLISNYAANAGKSGGEFFTPQSVSKLIARLAVHGQEK
                     VNKIYDPACGSGSLLLQAKKQFDEHIIEEGFFGQEINHTTYNLARMNMFLHNVNYNKF
                     HIELGDTLTNPKLKDSKPFDAVVSNPPYSIDWIGSDDPTLINDDRFAPAGVLAPKSKA
                     DFAFILHALNYLSGRGRAAIVSFPGIFYRGGAEQKIRQYLVEGNYVETVIALAPNLFY
                     GTCIAVNILVLSKHKDNTDIQFIDAGGFFKKETNNNVLTEEHIAEIVKLFADKADVPH
                     IAQNAAQQTVKDNGYNLAVSSYVEAEDTREVIDIRQLNAEISETVAKIERLRREIDEV
                     IAEIET"
     gene            complement(29710..30714)
                     /gene="rfaD"
                     /locus_tag="WNA25_000029"
     CDS             complement(29710..30714)
                     /gene="rfaD"
                     /locus_tag="WNA25_000029"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFA9822909.1"
                     /translation="MTIIVTGAAGFIGSNIVKALNQRGITDIVAVDNLTKGEKFKNLA
                     ECEIAHYLDKHEFIRQVREHILPYQNIEAVFHQGACSDTMNHDGLYMMENNYQYTLDL
                     LDWCQDERIPFLYASSAAVYGKGEIFREERELEKPLNVYGYSKFLFDQVLRRRMKEGL
                     TAQVVGFRYFNVYGQHEQHKGRMASVAFHHFHQYREHGYVNLFGSNDGYGNGEQTRDF
                     VSVEDVAKINLYFFDHPELSGIYNLGTGRSQQFNELAAATVNACRAAEGKSELSLKEL
                     VEEELIRYIPFPDALKGKYQGFTQADITKLREAGYKEEFFDVKAGVNRYVKWMLENLA
                     "
     gene            complement(30778..31740)
                     /gene="hldA"
                     /locus_tag="WNA25_000030"
     CDS             complement(30778..31740)
                     /gene="hldA"
                     /locus_tag="WNA25_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose synthase"
                     /protein_id="HFA9822910.1"
                     /translation="MPAKFQQETLKSRFAQAKVLVVGDVMLDRYWFGDVSRISPEAPV
                     PVAKIGRIDQRAGGAANVARNIASLGGRAGLLSVTGDDEAAAALDALMVQDGVASYLM
                     RDKQIATTVKLRVVARNQQLIRLDFEEHPNREVLEQIKRKYREILPEYDAIIFSDYGK
                     GGLSHISDMIDWAKHVGKTVLIDPKGDDYEKYVGATLITPNCAELKEVVGSWKNEGDL
                     TEKAQNLRRHLDLTAVLLTRSEEGMTLFSEGEPIYQPTRAQEVYDVSGAGDTVIAGMG
                     LGLAAGCTMPEAMYLANTAAGVVVAKLGTAVCSFAELVEALDGQ"
     gene            complement(31796..32536)
                     /gene="pyrF"
                     /locus_tag="WNA25_000031"
     CDS             complement(31796..32536)
                     /gene="pyrF"
                     /locus_tag="WNA25_000031"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFA9822911.1"
                     /translation="MNPLITDFQTPQQRTPVIVALDFANEKDTLGFVRNLDPALCQIK
                     IGKELFTATGRSLAESLIHQGFKLFLDLKYHDIPHTVAQACKVAADMGVWMVDMHASG
                     GRRMMEAAAEAVAGYGTKPLLIGVTVLTSMEQSDLAEIGLNTAPEEQVIRLAKLAQSS
                     GLDGVVCSAQEAAPLRRELGRDFVLVTPGIRLDVAGNNDDQRRIMTPAEALAAGSTYL
                     VMGRPVTRAADPVAVLREVNRVANLEAN"
     gene            complement(33060..33707)
                     /gene="adk"
                     /locus_tag="WNA25_000032"
     CDS             complement(33060..33707)
                     /gene="adk"
                     /locus_tag="WNA25_000032"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFA9822912.1"
                     /translation="MKALLLGAPGAGKGTQAQFITAAFGIPQISTGDMLRAAIKAGTP
                     LGLEAKKIIDEGGLVRDDIIIGMVKERIAQDDCKNGFLFDGFPRTLAQAEAMVEAGVG
                     LDAVVEIDVSDSVIVDRMSGRRVHLASGRTYHVTYNPPKTEGKDDVTGEDLIQRDDDK
                     EETVKKRLAVYHEQTEVLVDFYSKLEGEHAPKYIKVDGTQAVEAVKAEVLGALGK"
     gene            33922..34761
                     /gene="htpX"
                     /locus_tag="WNA25_000033"
     CDS             33922..34761
                     /gene="htpX"
                     /locus_tag="WNA25_000033"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFA9822913.1"
                     /translation="MKRIFLFLATNIAVLVVINIVLAVLGINSRGGAGSLLAYSAVVG
                     FTGSIISLLMSKFIAKQSVGAEVIDTPRTEEEAWLLNTVEAQARQWNLKTPEVAIYHS
                     PEPNAFATGASRNSSLIAVSTGLLDHMTRDEVEAVLAHEMAHVGNGDMVTLTLIQGVV
                     NTFVVFLSRIIANLIARNNDGSQSQGTYFLVSMVFQILFGFLASLIVMWFSRQREYRA
                     DAGAAKLVGAPKMISALQRLKGNPVDLPEEMNAMGIAGDTRDSLLSTHPSLDNRIARL
                     KSL"
     gene            complement(34997..36295)
                     /locus_tag="WNA25_000034"
     CDS             complement(34997..36295)
                     /locus_tag="WNA25_000034"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFA9822914.1"
                     /translation="MAKNVVVIGAQWGDEGKGKIVDWLAEEAGGVVRFQGGHNAGHTL
                     VVGGKKTILRLIPSGILHEGLDCFIGSGVVVSPEALLGEIDELNAAGVKNVEGRLKIA
                     PTCPLILPYHIALDQAREASRGKGKIGTTGRGIGPAYEDKVARRAIRAADLLHPEKLR
                     EKLDAVLAYYNVQLQYLHNAGPVKAEDVMAVIEKVAPRIAPMIADVSRVLNEKNKNGE
                     KLLFEGAQGALLDIDYGTYPFVTSSNCLAGAASAGAGVGPQMLDYVLGIVKAYTTRVG
                     SGPFPTELFDEVGAGLAERGHEFGSVTGRARRCGWFDAAALKRSIQINGISGMCITKL
                     DVMDGVETINICVGYELPGGGKTDILPCGSDAVETCKPIYETMPGWRESTVGVKSYDA
                     LPANAKAYLKRIEEVCGAPVAIVSTGPDREETIVLHHPFA"
     gene            complement(36400..37551)
                     /locus_tag="WNA25_000035"
     CDS             complement(36400..37551)
                     /locus_tag="WNA25_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HFA9822915.1"
                     /translation="MQTWQLPEHIADVLPTNARQLESAREQLLALFRVHGYELVQPPL
                     MEYAHSLLTHIDAGLSLKTILVTDRLSGRQLGIRADITPQVARIDAHLLSANQGINRL
                     CYAGPVLHAQPDGLPNMREPLQAGAEMYGFADIRGDIELIDLMLKSMKIADMGKVLLS
                     LGHIGIFRALSDAAHLDAGQSATLLALMQDKDTGSVEAQVKAWKLDGMWAKAFSLLPR
                     LYGGREVLSDARGRLPDLSAVGGALDELQAVCDAFPDNEIHIDLSELRVDNYHTGLLY
                     AAYAADFHDAVARGGRYDGLGGYFGRARPATGFSFDLRSFIGRLPAVERQPAVLVDAE
                     DAEAAREAVEALREQGQCVVIDYGIGHNVSEELAGRLKKTDGVWQIVKR"
     gene            37886..39265
                     /locus_tag="WNA25_000036"
     CDS             37886..39265
                     /locus_tag="WNA25_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     NorM"
                     /protein_id="HFA9822916.1"
                     /translation="MLLDLDRFSFSVFLKEIRLLTALALPMLLAQVAQVGIGFVDTVM
                     AGGAGKEDLAAVALGSSAFATVYITFMGIMAALNPMIAQLYGAGKTGEAGETGRQGIW
                     FGLILGIFGMILMWAAITPFRNWLTLSDYVEGTMAQYMLFTSLAMPAAMVHRALHAYA
                     SSLNRPRLIMLVSFAAFVLNVPLNYIFVYGKFGMPALGGAGCGVATMAVFWFSALALW
                     IYIAKEKFFRPFGLTAKFGKPDWAVFKQIWKIGAPIGLSYFLEASAFSFIVFLIAPFG
                     EDYVAAQQVGISLSGILYMIPQSVGSAGTVRIGFSLGRREFSRARYISGVSLVSGWVL
                     AVITVLSLVLFRSPLASMYNDDPAVLSIASTVLLFAGLFQPADFTQCIASYALRGYKV
                     TKVPMFIHAAAFWGCGLLPGYLLAYRFDMGIYGFWTALIASLTIAAVALVWCLEKYSM
                     ELVKSHKAV"
     gene            39309..40349
                     /gene="murB"
                     /locus_tag="WNA25_000037"
     CDS             39309..40349
                     /gene="murB"
                     /locus_tag="WNA25_000037"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFA9822917.1"
                     /translation="MQPIRYRTDLTPYNTFGLHAQARAFIALKHADELRDIVRLPEFD
                     RDTVLWLGGGSNILLMQDYDGLVVHMENKGIREIARSDGMVLIEAQAGEIWHDFVLHT
                     VALGLSGLENLSLIPGTVGASPVQNIGAYGVEAKDVIHSVRCFDLDTETFVTLSNADC
                     RFAYRESLFKQEGKGRYVIVSVVFALKTHFVPNLGYGDLAAKVAELSAGREATAKDVS
                     DAVSAIRNSKLPDPKVLGNVGSFFKNPVVSAEKAATLLQRHPDMPRYPQPDGSVKLAA
                     GWLIDQCRLKGFQIGGAAVHDRQALVLANKNNASSDDIWKLAQHVCNTVFTRFQVELH
                     AEPNWLPASFSL"
     gene            40361..41011
                     /locus_tag="WNA25_000038"
     CDS             40361..41011
                     /locus_tag="WNA25_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFA9822918.1"
                     /translation="MPVTRIAKTNTYTRIIDASLALFNEEGERNISTNHIAAHLGISP
                     GNLYYHFRNKDEIIVQLFKRYSEALLEYLNEAVLPSDVEDSINYMAGIYDVMWEYRFL
                     FSDVNTLLARSAELLGEHNTFTQAKVSPLLVKLLTQLNGLNVINADQTAMNDLAVNMW
                     MVTKYWFDFDSSLRGRTKLTEDSKARGVRRTLSLLRPYLLPEHRAEYDRKISNGNP"
     gene            41066..41842
                     /locus_tag="WNA25_000039"
     CDS             41066..41842
                     /locus_tag="WNA25_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFA9822919.1"
                     /translation="MRAVPPPHISITGLGYLGLPLAQKFYRHGSRVAAIKRSLTSDDI
                     NLPIHLDTFDLNRTDTFQTALWRHHADKPVWFFLLPPSFLAHYADTVKQWAELARACN
                     VQHLIFTSSTSVYGDKARECNETAAPDPQTESARQILAAEQHLLDNGVPNIDILRLGG
                     LYCAERHPVSRLVQKQNIQGGNRPVNIVHRDIAVENLFQTASNPGGRRLKNIIEPRHP
                     TRREFYTEEAAKLGLPAPDFSPDDSVGKIIRTVCDNGLSL"
     gene            41892..42458
                     /locus_tag="WNA25_000040"
     CDS             41892..42458
                     /locus_tag="WNA25_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFA9822920.1"
                     /translation="MSALLPIINRLILQSPDSRSELTSFAGKTLTLNIAGLKLAGRIT
                     EDGLLSAGNGFADTEITFRNSAIRKILQGGEPGAGDIRLEGDLILGIAVLSLLGSLRS
                     RASDELARIFGTQAGIGSRATDIGHGIKQIGRNIAEQIGGFSREPESANTGNEALADC
                     LDEISRLRDGVERLNERLDRLERDIWID"
     gene            42478..43368
                     /locus_tag="WNA25_000041"
     CDS             42478..43368
                     /locus_tag="WNA25_000041"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFA9822921.1"
                     /translation="MNSPFHNIGIVTRPNTPDIQDTAHTLITFLKQHGFTVYLDEVGV
                     RECCIYTQDTDGCHIVNKTELGQYCDLVAVLGGDGTFLSAAREITPRAVPIIGINQGH
                     LGFLTQIPREYMTDKLLPVLEGKYLAEERILIEAALIREGKTAERALALNDAVLSRGG
                     AGQMIEFEVFVNQEFVYTQRSDGLIVSTPTGSTAYSLAAGGPIMQAGLHAFTLVPICP
                     QSMTNRPIAIPDTSEIEILVTQGGDARVHFDGQSFIDVQNLDRIIIRRYHNPLRILHP
                     TDYQYFKTLRQKLHWGEQLV"
     gene            43393..44151
                     /locus_tag="WNA25_000042"
     CDS             43393..44151
                     /locus_tag="WNA25_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HFA9822922.1"
                     /translation="MNSKISSDHTEDTIRLSKRMAQLGLCSRREADGHIEQGWVTVNG
                     KTAVLGQKVSPADRIELNKKAHEQQAARITILLNKPVGYVSAQAEKGYKSAAELITPE
                     NCWEGDTSRISFDPKHKIGLAPAGRLDIDSVGLLVLTQDGRIAKQLIGENSGSEKEYL
                     VRVRGKLDEKGLALLNHGLSLDGDKLRPAKVEWQNEDQLRFVLKQGKKRQIRRMCELV
                     GLRVVGLKRIRMGKVKLGRLPPGKWRYLAPGESF"
     gene            complement(44206..44870)
                     /locus_tag="WNA25_000043"
                     /pseudo
     CDS             complement(44206..44870)
                     /locus_tag="WNA25_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357347.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
     gene            complement(45067..45864)
                     /locus_tag="WNA25_000044"
     CDS             complement(45067..45864)
                     /locus_tag="WNA25_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE2"
                     /protein_id="HFA9822923.1"
                     /translation="MNIKDKQRMNAIADVQSSRDLRNLPINQVGIKDLRFPITLKTAE
                     GTQSTVARLTMTVYLPAEQKGTHMSRFVALMEQHTEVLDFAQLHRLTAEMVALLDSRA
                     GKISVSFPFFRKKTAPVSGIRSLLDYDVSLTGEMKDGAYGHSMKVMIPVTSLCPCSKE
                     ISQYGAHNQRSHVTVSLTSDAEVGIEEVIDYVETQASCQLYGLLKRPDEKYVTEKAYE
                     NPKFVEDMVRDVATSLIADKRIKSFVVESENFESIHNHSAYAYIAYP"
     gene            45901..46047
                     /locus_tag="WNA25_000045"
     CDS             45901..46047
                     /locus_tag="WNA25_000045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9822924.1"
                     /translation="MPSEKTLSAVLPANAGLYHLLLAAALIVSIFGIVIKSRLIHILQ
                     GRNR"
     gene            46044..47201
                     /locus_tag="WNA25_000046"
     CDS             46044..47201
                     /locus_tag="WNA25_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HFA9822925.1"
                     /translation="MKFPTRAIHSGYDCDEHNRALMPPIYQNSMFALHEIGENVPYRY
                     SRLSNPTRQILEDTVADLEHGAAGFAFSSGMAGIDAVWRTFLRPGDTIVAVADIYGGA
                     YDLLVDVYQKWGVNVVFADLGNPDNLDELLKAHNVKLVWLEMPSNPLLRLVDIKALAA
                     KAKAADALVGIDNTFATPYLQQPLDMGCDFAFHSATKYLCGHSDVLMGIVVAKTKELA
                     QPLHDMMVHTGAIAGPTDCWLVLRGIKTLALRMEAHCKNALEIARRLEAHPAIEKVFH
                     PGLPSHEHYALAQAQMPKGIGGVVTVYLKNDTREAANSVIKNMKLVKMTSSLGGVESL
                     VNHCYSQSHSGVPHNVKMEMGIRVGLLRFSIGIEDVDDIWNDISAALDTTL"
     gene            complement(47265..48266)
                     /gene="hemB"
                     /locus_tag="WNA25_000047"
     CDS             complement(47265..48266)
                     /gene="hemB"
                     /locus_tag="WNA25_000047"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFA9822926.1"
                     /translation="MQFPYRNVPASRMRRMRRDDFSRRLMREHMLTADDLIYPVFVLE
                     GAAREEDVPSMPGVKRQSLDRLLFTAEEAVKLGIPMLALFPVVTANKTGRAQEAYNPE
                     GLVPSTVRALRERFPELGIMTDVALDPYTVHGQDGLTDENGYVMNDETVEVLVKQALC
                     HAEAGTQVVAPSDMMDGRIGAIREALEDAGHIHTRIMAYSAKYASAFYGPFRDAVGSS
                     GNLGKADKKTYQMDPANTDEALHEVALDIQEGADMVMVKPGLPYLDVVRRVKDEFGVP
                     TYAYQVSGEYAMLQAAVANGWLDGGKVVLESLLAFKRAGADGILTYYAIEAAKMLKR"
     gene            complement(48450..48734)
                     /gene="yhbY"
                     /locus_tag="WNA25_000048"
     CDS             complement(48450..48734)
                     /gene="yhbY"
                     /locus_tag="WNA25_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFA9822927.1"
                     /translation="MADTKLNTKEILELKARAHHLHPVVMVGQQGLTDAVIKETDAAL
                     TAHELIKVRVFGDDRAERIEICNTLCEAVDAQPVRHIGKLLVLWRKNIEA"
     gene            48844..49464
                     /locus_tag="WNA25_000049"
     CDS             48844..49464
                     /locus_tag="WNA25_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HFA9822928.1"
                     /translation="MAVRSKSSKAWLHEHINDQYVHMAQKDGYRARAAYKLLEINEKD
                     KIIKPGTVLADLGSAPGSWSQVAAKLTGTSGAVFALDILPMEAIGGVSFIQGDFREND
                     VLAQFETLLDNRPLDLVICDMAPNMSGNAVSDQARSFYLCELALDFASQHLKTGGSFL
                     VKVFQGAGYQEYMAAMREIFGTVQTRKPEASRNRSSEIYLLGKNKR"
     gene            49528..51495
                     /gene="ftsH"
                     /locus_tag="WNA25_000050"
     CDS             49528..51495
                     /gene="ftsH"
                     /locus_tag="WNA25_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFA9822929.1"
                     /translation="MGNTFKSILVWVALGIGLMAAFNALDGKKEDNGQIEYSQFIRQV
                     NNGEVSGVNIEGSVVSGYLIKGERTDKSTFFTNAPLDDNLIKTLLDKNVRVKVTPEEK
                     PSALAALFYSLLPVLLLIGAWFYFMRMQTGGGGKGGAFSFGKSRARLLDKDANKVTFA
                     DVAGCDEAKEEVQEIVDYLKAPNRYQSLGGRVPRGILLAGSPGTGKTLLAKAIAGEAG
                     VPFFSISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRQRGAGLGGGN
                     DEREQTLNQLLVEMDGFESNQTVIVIAATNRPDVLDPALQRPGRFDRQVVVPLPDIRG
                     REQILNVHSKKVPLDESVDLLSLARGTPGFSGADLANLVNEAALFAGRRNKVKVDQSD
                     FEDAKDKIYMGPERRSMVMHEDEKRATAYHESGHAIVAESLPFTDPVHKVTIMPRGRA
                     LGLTWQLPERDRISMYKDQMLSQLSILFGGRIAEDIFVGRISTGASNDFERATQMARE
                     MVTRYGMSDKMGVMVYAENEGEVFLGRSVTRSQNISEKTQQDIDAEIRRILDEQYQVA
                     YKILDENRDKMETMCKALMEWETIDRDQVLEIMAGKQPSPPKDYSHNLRENADAAEDN
                     APHAPTREKTEAPAPADTASTESGQQPENKA"
     gene            51717..52148
                     /locus_tag="WNA25_000051"
     CDS             51717..52148
                     /locus_tag="WNA25_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HFA9822930.1"
                     /translation="MKKVEKNILVLHGADKMFELVDKVEDYPHFLPWYSKTEVIGRSG
                     NELKARLFMDYMRVRQSFATHNRNIPGREIRMELLEGPFKTLRGTWKFIDLGDDMCKI
                     EFNLEYDFSNAVLSALISPVFNHLSATLVEAFVKEADRRYA"
     gene            52141..52419
                     /locus_tag="WNA25_000052"
     CDS             52141..52419
                     /locus_tag="WNA25_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFA9822931.1"
                     /translation="MLEIEIVYGLPDRQVLKTMQLAEGTTVRTAALQSGLDGIFENLN
                     LHSAPLGIFGKAVKDDTPLRDGDRIEVYRPLLIDPKEARRKRVQNQEE"
     gene            52472..53050
                     /gene="pth"
                     /locus_tag="WNA25_000053"
     CDS             52472..53050
                     /gene="pth"
                     /locus_tag="WNA25_000053"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFA9822932.1"
                     /translation="MSNTIKMVVGLGNPGKEYEQTRHNAGFWFLDELAWKWKASFKEE
                     KKFFGEVARAALPDGDVWLLKPATFMNRSGQAVAALAQFYKIKPEEILVVHDELDIPC
                     GRIKFKLGGGNGGHNGLKDIQAKLGTADYYRLRLGIGHPGDRNLVVGYVLNKPSAEHR
                     RQIDDAVAKSLQAVPDIISGKWEEATRFLHSK"
     gene            53111..53317
                     /locus_tag="WNA25_000054"
     CDS             53111..53317
                     /locus_tag="WNA25_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HFA9822933.1"
                     /translation="MTELKQLIQTESIPVIEETLDFLLYECSIDDAPSAEEVAQWRDI
                     LAARGGKFLRLSKICQTWLDEEAA"
     gene            53314..53676
                     /locus_tag="WNA25_000055"
     CDS             53314..53676
                     /locus_tag="WNA25_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFA9822934.1"
                     /translation="MKLPRNRFSLLSALWFAGGIYSLLFKAADTAPPPFPHFDKAAHL
                     ALFFAQILLLAKAFKTGKLPIPYRSLIAFAFCFAVGSECAQAWFTATRTGSLGDVLAD
                     LTGAALALFAARSACRPD"
     gene            53905..55320
                     /locus_tag="WNA25_000056"
     CDS             53905..55320
                     /locus_tag="WNA25_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13/DASS family transporter"
                     /protein_id="HFA9822935.1"
                     /translation="MNLHAKDKTQHPENVELLSAQKPITDFKGLLTTIISAVVCFGIY
                     HILPYSPDANKGIALLIFVAALWFTEAVHITITALMVPILAVVLGFPDMDIKKAMADF
                     SNPIIYIFFGGFALATALHMQRLDRKIAVSLLRLSRGNMKVAVLMLFLVTAFLSMWIS
                     NTATAAMMLPLAMGMLSHLDREKEHKTYVFLLLGIAYCASIGGLGTLVGSPPNLIAAK
                     ALNLDFVGWMKLGLPMMLLILPLMLLSLYVILKPNLNERVEIKAESIPWTLHRVIALL
                     IFLATAAAWIFGSKIKTAFGISNPDTVIALSAAVAVVVFGVAQWKEVARNTDWGVLML
                     FGGGISLSTLLKTSGASEALGQQVAATFSGAPAFLVILIVAAFIIFLTEFTSNTASAA
                     LLVPIFSGIAMQMGLPEQVLVFVIGIAASCAFMLPVATPPNAIVFGTGLIKQREMMNV
                     GILLNILCVVLVALWAYAVLM"
     gene            55440..55949
                     /locus_tag="WNA25_000057"
     CDS             55440..55949
                     /locus_tag="WNA25_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFA9822936.1"
                     /translation="MIILHTNKGDIKIELDFDKAPVTAKNFEQYVKDGFYDGVIFHRV
                     IKGFMIQGGGMDENMNEKETRDPIQNEASNGLPNDKYTIAMARTSDPHSAGAQFFINT
                     ADNAFLNFRSKELYGKTVVQDWGYAVFGKVVDGFDVVDAIESVSTKRHGYHDDVPTEP
                     VIIIKAEAV"
     gene            56117..57499
                     /locus_tag="WNA25_000058"
     CDS             56117..57499
                     /locus_tag="WNA25_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFA9822937.1"
                     /translation="MASITRDIFKAYDIRGIVGKTLTDDAAYFIGRAIAAKAAEKGIA
                     RIALGRDGRLSGPELMEHIQRGLTDSGIGVLNVGMVATPMLYFAAVNECGGSGVMITG
                     SHNPPDYNGFKMMLGGDTLAGEAIQELLAIVEKDGFVAADKQGSVTEKDISGAYHDHI
                     VGHVKLKRPINIAIDAGNGVGGAFAGKLYKGLGNEVTELFCEVDGNFPNHHPDPSKPE
                     NLQDLIAALKNGDAEIGLAFDGDADRLGVVTKDGNIIYPDRQLMLFAQDVLNRNPGAK
                     VIFDVKSTRLLAPWIKEHGGEAIMEKTGHSFIKSAMKKTGALVAGEMSGHVFFKERWF
                     GFDDGLYAGARLLEILSASDNPSEVLDNLPQSISTPELNISLPEGSNGHQVIEELAAK
                     AQFEGATEIITIDGLRVEFPDGFGLMRASNTTPILVLRFEADTQAAIERIQNRFKAVI
                     ESNPHLIWPL"
     gene            complement(57555..58310)
                     /locus_tag="WNA25_000059"
     CDS             complement(57555..58310)
                     /locus_tag="WNA25_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFA9822938.1"
                     /translation="MIKIRNIHKTFGENTILRGIDLDVGKGQVVVILGPSGSGKTTFL
                     RCLNALEMPEDGQIEFDNARPLRIDFSKKTSKHDILALRRKSGMVFQQYNLFPHKTVL
                     ENVMEGPVAVQGKPAAQAREEALKLLEKVGLGDKVDLYPYQLSGGQQQRVGIARALAI
                     QPELMLFDEPTSALDPELVQDVLDAMKELAREGWTMVVVTHEIKFTLEVATNVVVMDG
                     GVIVEQGSPKELFDHLKHERTRRFLSQIQSAKI"
     gene            complement(58320..59036)
                     /locus_tag="WNA25_000060"
     CDS             complement(58320..59036)
                     /locus_tag="WNA25_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFA9822939.1"
                     /translation="MFNNFLASLPFMTETRADMLISAFWPMVKAGFTVSLPLAIASFV
                     IGMIIAVAVALVRIMPSGGIFQKCLLKLVEFYISVVRGTPLLVQLVIVFYGLPSVGIY
                     INPIPAAIIGFSLNVGAYASETIRAAILSVPKGQWEAGFSIGMTYMQTFRRIVAPQAF
                     RVAVPPLSNEFIGLFKNTSLAAVVTVTELFRVAQETANRTYDFLPVYIEAALVYWCFC
                     KVLFLIQARLEKRFDRYVAK"
     gene            complement(59026..59853)
                     /locus_tag="WNA25_000061"
     CDS             complement(59026..59853)
                     /locus_tag="WNA25_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFA9822940.1"
                     /translation="MMLKKFVLGGIAALVLAACGGSEGGSGASSAPAQSAISGSLIER
                     INNKGTVTVGTEGTYAPFTYHDKDGKLTGYDVEVTRAVAEKLGVKVEFKETQWDSMMA
                     GLKAGRFDVVANQVGLTSPERQATFDKSEPYSWSGAVLVAHNDSNIKSIADIKGVKTA
                     QSLTSNYGEKAKAAGAQLVPVDGLAQSLTLIEQKRADATLNDELAVLDYLKKNPNAGV
                     KIVWSAPADEKVGSGLIVNKGNDEAVAKFSTAINELKADGTLKKLGEQFFGKDISVQ"
     gene            60138..61046
                     /locus_tag="WNA25_000062"
     CDS             60138..61046
                     /locus_tag="WNA25_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HFA9822941.1"
                     /translation="MNTPQSAIIPDHAQAGIFIEADFAANRLNDIKAACRASLDALSA
                     LKARFPDDILGLTIAFGSKAWATFGHTDEGSEIKPFPEMGNGLAPSTQHDMSIHIQSF
                     RQNAAYALAQSVLGAFGDSICVASEEHGLRLYQDRGLDGFVDGTENPQGDETIREVAI
                     IPEGLPDAGGSYVLLQKYLHDLKKWDAVPVAEQEASVGRSKETDDEFSRDVRLPDSHL
                     GRVNLKENGVGLKIVRRSLPFGKISGEHGLMFTAYCRTLHNIEAQLLSMFGDTDGKTD
                     LLLRHLSAAVSGGYYYAPSVERLQNL"
     gene            61123..64725
                     /gene="recB"
                     /locus_tag="WNA25_000063"
     CDS             61123..64725
                     /gene="recB"
                     /locus_tag="WNA25_000063"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFA9822942.1"
                     /translation="MSAPIRAFDPLTAPISGTNLIEASAGTGKTYGIAALFTRLIVLE
                     QKSVERVLVVTFTKAATAELKTRLRARLDDVLQVLESKEIAELGDDTLSDGIAAYCAE
                     HHEGDTFLPALLEQALQKESRTRLIVRLKAAIGQFDNAAIYTIHGFCQRILRDYAFLC
                     QAPFDVELTEEDGDRLLVPAQDFWRERVSGDPVLAALAFKRKAVPQTVLAQIRAYLSR
                     PYLNFRRPQADLKQAQRDAETSWQTVCRLLPELEAGFWRIHPDLNGNSYRKNSFGNLF
                     KELAQKSAAGQLPFLDKDTHERLLKLSSDKLEAGLKKGKTPDAAVFAELQKLADFGRD
                     LNALEEAEETTMIRLQLDLIEYLNSSLAEMKKSRRERGFDDLLLDVHTALTDNPHAET
                     PARAVAENWEIALIDEFQDTDPLQYEIFQKIFIARNRPLFLVGDPKQAIYSFRGADIY
                     AYLQAAEDARHRYTLATNYRSHAALIGSIGALFRLKERPFVLENIGYSEVGAARAESR
                     LSPKRPAVQLRWLHENDNEKANKDVLRRRAADYCADEIARALNEASGGRLNFKGRPLQ
                     SGDIAVLVRTHNEAVMISAALKKRQVQSVLLSRESVFASPEAAALSALIGFWLEPRRA
                     GTLRFVLTSGIFGYDAQQLHDFNQNESEILHWAESARTALDIWQKYGIFAAMQQFSQT
                     HGIETRLLSRNNGRSLTNYFQLLELLAAEDAQNRNPAALHKWLRDQISLAGNNGGDNR
                     AIRLESDEDLVKIVTMHASKGLQYPLVYCPFAWDAQDTGPSDWQILHQGANRAELLAK
                     AQLSESEQKQYADEEMAERLRLLYVALTRAEEQLNIYAAYSSDTADNPLAYLIEGLPQ
                     DSRETVRRTYACEKDGITMLKRNWRRVADNAPAGTDFAFTEDAPPPAAYRGNADQAAE
                     FAANSIPERGFRFVRHTSFTALSRHTQTPDGGEEDACPSLDAAETSVPAMPSETPTAS
                     DGISIHDFPKGTQAGLCLHEILEDFKFGQAAAEQETLIADKLKKYGFEEIWLPAVAEM
                     AEACRKTPLTGAYGLSDIPPGCRRPEMGFTLHTEDFGLKRLRDWLARDDIRLPEVCRA
                     AAETLDFHTVNGFLNGFIDMVCQDPDGNICIIDYKSNHLSAYTRQAMDEAVAHQHYYL
                     QALIYAVAAARYFKLRGQPPAAVSVRYLFLRGLDGKGGGVWRWDIDAAALEQIK"
     gene            64764..65123
                     /locus_tag="WNA25_000064"
     CDS             64764..65123
                     /locus_tag="WNA25_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFA9822943.1"
                     /translation="MNIKQLITAALIASAAFATQAAPQKPVSAAQTAQHSAVWIDVRS
                     EQEFSEGHLHNAVNIPVDQIVRRIYEAAPDKDTPVNLYCRSGRRAEAALQELKKAGYT
                     NVANHGGYEDLLKKGMK"
     gene            65248..65727
                     /locus_tag="WNA25_000065"
     CDS             65248..65727
                     /locus_tag="WNA25_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HFA9822944.1"
                     /translation="MKHILPLIAASALCISTASAHPADKPPTQNETAMTTHTLTSKYS
                     FDETVSRLETAIKSKGMDIFAVIDHQEAARRNGLTMQPAKVIVFGTPKAGTPLMVKDP
                     AFALQLPLRVLVTETDGKVRTAYTDTRALIVGSRISFDEVANTLANAEKLIQKTVGE"
     gene            complement(66002..67381)
                     /gene="radA"
                     /locus_tag="WNA25_000066"
     CDS             complement(66002..67381)
                     /gene="radA"
                     /locus_tag="WNA25_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFA9822945.1"
                     /translation="MAKTLKTLYQCTECGGTSPKWQGKCPHCGEWNTLQESFAAPEPK
                     NARFQSWAADTSTVQSLSAVTAAEVPRNPTGMGELDRVLGGGLVDGAVILLGGDPGIG
                     KSTLLLQTIAKMAQSRKVLYVSGEESAQQVALRSQRLELHAEGVNLLAEIRMEAIQAA
                     LKQHQPEVVVIDSIQTMYSDQITSAPGSVSQVRECAAQLTRMAKQMGIAMILVGHVTK
                     DGAIAGPRVLEHMVDTVLYFEGDQHSNYRMIRAIKNRFGAANELGVFAMTENGLKGVS
                     NPSAIFLASYRDDTPGSCVLVTQEGSRPLLVEIQALVDDAHGFTPKRLSVGLEQNRLA
                     MLLAVLNRHGGIACFDQDVFLNAVGGVKIGEPAADLAVILAMLSSFRNRPMPEKTVVF
                     GEIGLSGEVRPVARGQERLKEAEKLGFKRAIVPKANMPRNAKEFPNLKIHGVSSLQEA
                     IDICRDSRE"
     gene            67723..68847
                     /locus_tag="WNA25_000067"
     CDS             67723..68847
                     /locus_tag="WNA25_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFA9822946.1"
                     /translation="MLSKQISNLNSSSNKPKILSLFSGCGGLDLGFHQAGCETVWAND
                     FSHWACESFRKNIGDVIVEGDIEQINPNDPTIPDCDIILGGFPCQDFSMIWKQPGLEG
                     ERGNLYKSFLRFVNAKKPKVFVAENVKGLLTANKKKAIQQIITDFENCGYYVQAKLYN
                     FAEFGVPQFRERVLIVGVRLDTGFDFRHPEPTHNETGENGLKPYVTAGQAISNIPQNA
                     SNNELLKISDKTRRMLELIPEGGNFTDIPKDHPLYVKGMISHVYRRMHRNEPSKTIIA
                     AGGGGTWGYHFPEPRAFTNRERARLQSFPDDFEFVGSTTEVRRQIGNAVPPQGVVELA
                     KSILPIFSDNYEKVDLHEKLVEEKEILFHDRLSKIRGGKQ"
     gene            68844..69881
                     /locus_tag="WNA25_000068"
     CDS             68844..69881
                     /locus_tag="WNA25_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoFVII family restriction endonuclease"
                     /protein_id="HFA9822947.1"
                     /translation="MNTVFSNIANAKITEKSLNAVWMDLFKSADEVLMATGYVSNDAV
                     VELHKILELNDHIQKIDLLVGMHYLEGFSHLQYDSLCKLNDFLRHEKRGAVYVSPFVK
                     FHGKMYSFKNYQKINGLIGSANLTCFWDSTERTYETMLHLNGKPAQILQADIQSTIHK
                     LGKNIQEVERPSKFIEHNSHLENCLGVQKIAPEQIRQLFAQTSEYHFSIPAKTEEKSN
                     LNVFFGEGRRDKRGFVKPRPWYEVELIVSKDITSQEGYPVLKSFTVITDDGWQFQCKT
                     SGDYSKNFRSENDLKTLGKWIKGRLESHGCLQNNEKITHETLREYGNDHFELRSTDNP
                     DVWLLSFKGKN"
     gene            69882..71771
                     /locus_tag="WNA25_000069"
     CDS             69882..71771
                     /locus_tag="WNA25_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFA9822948.1"
                     /translation="MLRTYLNQLTPPELADSVKNTVDGFMEKLSQTEPKIAQNVLLLG
                     NVQSGKTAQVLGVLSALADDGDHKVFLYLTTDSVDLQDQTVKRAKANLKNFIVLSEAD
                     DRSFMEVMKAENPILVVIKKNARVLKRWRNLFASQSSLKGYPLVIVDDEADAASLNTN
                     SDKPAKDASTINKLLNDIKNSCCQSLFIQLTATPQSLLLQHEESDWQPEFIHFFEAGE
                     KYIGGNFVFSDPPSYIVRFIDSELDDMKDESGEIAEGAKQALLSFLITCAEFALCDKA
                     NCNFALHPSYKIQDHQAFSKKIQAFLNDLVQAVNNGEDLAGSFKESYLDLQKTKPDIH
                     HFDEIYEKLTALLENKQISTLVVNSQTETDFDLEKGFNIIIGGNVIGRGLTIPKLQTV
                     YYSRTAKKPNADTFWQHSRIFGYDRDKSLLRLYIPFDVYYFFVQLNQANNLIIGQAKN
                     SGGNIQVIYPKNINPTRKNVLKFDSINQIVGGVNYFPLHPNEDNLSEINKILPSILKD
                     EIQSDLYQIDIEDLFLVLDKLGRYVPDDWNKEKFIAGVEALKAQRPSFKTYVLIKTGR
                     KLSRATGTMLSEDDRKLGEKYPNDLFLTLYQVVGNKDKGWQGKDFWLPNIKLPHNGLV
                     YQSAK"
     gene            complement(71834..72898)
                     /locus_tag="WNA25_000070"
     CDS             complement(71834..72898)
                     /locus_tag="WNA25_000070"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFA9822949.1"
                     /translation="MTLLKNDTFLRALLKQPVEYTPIWMMRQAGRYLPEYKATRAKAG
                     SFLDLCKNTGLATEVTIQPLERFDLDAAILFSDILTVPDAMGLGLYFAEGEGPKFKRA
                     LQHESDIAKLHVPDMEKLQYVFDAVTSIRKALDGRVPLIGFSGSPFTLACYMVEGGGS
                     KEFRTIKTMMYSRPDLLYKILDTNAQAVTAYLNAQIDAGAQAVQIFDTWGGVLSDAAF
                     KEFSLKYIRQIVAGLKRESEGRRVPVIVFAKGGGLWLESMAQIGADALGLDWTCNIGE
                     ARRRVGNQVALQGNFDPSALFGTPESIRTEVARILTGYGHGSGHVFNLGHGINQHADP
                     EHAKILVDTVHELSRQYHGG"
     gene            complement(72973..74190)
                     /locus_tag="WNA25_000071"
     CDS             complement(72973..74190)
                     /locus_tag="WNA25_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HFA9822950.1"
                     /translation="MKTVVWIVVLFAAAVGLALASGIYTGDVYIVLGQTMLRINLHAF
                     VLGSLIAVVVWYFLFKFIIGVLNIPEKMQRSGSARKGRKAALALNKAGLAYFEGRFEK
                     AELEASRVLGNKEAGDNRTLALMLGAHAAGQMENIELRDRYLAEIAKLPEKQQLSRYL
                     LLAESALNRRDYEAAEANLHAAAKMNANLTRLVRLQLRYAFDRGDALQVLAKTEKLSK
                     AGALGKSEMERYQNWAYRRQMADAADAAALKTCLKRIPDSLKNGELSVSVAEKYERLG
                     LYADAVKWVKQHYPHNRRPELLEAFVESVRFLGEREQQKAIDFADSWLKEQPDNALLL
                     MYLGRLAYGRKLWGKAKGYLEASIALKPSIPARLVLAKVFDETAQSQKAEAQRNLVLA
                     SVAGENRPSAETR"
     gene            complement(74187..75521)
                     /locus_tag="WNA25_000072"
     CDS             complement(74187..75521)
                     /locus_tag="WNA25_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFA9822951.1"
                     /translation="MGEPENKSSEPVREIQASKEMPSETSSPRKENETEVHIPAAPFI
                     VKQSGSNALAACALVLAALGLGASGFLFVQGQNVLKNQELAFNQKIDKAALGESENAA
                     LLKDNLNRQSAIQSELDRLDSGVKANGEQILMTQKAYRELTKGRADWLVDETETILNL
                     AAQQLVLTGNIQTAVGVLEHIDSRLSRFDQAELLPIKQAVSSDLAELKNRPYVDISGT
                     ALRLDRLETAVSGLPLILDGVLKPGVQARNEAVSASWWQNVWEKSLGTLKGLVEIRRL
                     ENNDAMLISPEQAYFVRENLRLRLLDARTALMQRNGEVYQGDLNNAEAAVRQYFDAKS
                     PATQSWLKELAELKTLDVRMTADDGLKTSLNAVRAYRDGTRMTAAENQEAEQAASEPA
                     NENTASEPAAASDVKAIEAPSLPSERKPEQPAKKQPAPEKAGRSPSAKGERA"
     gene            complement(75533..76297)
                     /locus_tag="WNA25_000073"
     CDS             complement(75533..76297)
                     /locus_tag="WNA25_000073"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFA9822952.1"
                     /translation="METAKPVMLIVRPSGRAKDDVEVCRRAGWQAEVLSPIEIETDEA
                     SLKRLPEMYARADAVFWVSPAAVETAVPYLNLSDGIKAHIAVGQGSRRALARYAGAGV
                     FAPEDGNDSEAVLRLPVWNSLPEGARVLSVRGHGGRDFLMNALQEKGFRTEVAEVYFR
                     RHKPLNFQNFQTENIAAAYITSTELVQSLFAQLPPQFSRFFKSLLYFTHHPRIAEALK
                     REGVCSVETVPTLEAALSYSSISVSDGMVFPGTSNQ"
     gene            complement(76315..76737)
                     /locus_tag="WNA25_000074"
     CDS             complement(76315..76737)
                     /locus_tag="WNA25_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9822953.1"
                     /translation="MKKMFLSAALLLSAAAQTVWADTVFSCKTDNNKYIEVQKINRNL
                     YEYSFGSAAKKEIAIRNSKADLLGRSDRWQGMGSGRRATMKFQNGEFMYTVWTGFDSV
                     THTESSGVVVERRGKEVARVGCTPKTAQANFNDDDFSR"
     gene            complement(77019..77987)
                     /locus_tag="WNA25_000075"
     CDS             complement(77019..77987)
                     /locus_tag="WNA25_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFA9822954.1"
                     /translation="MVELQEIFDVSYGSKLDLNKMSSFNPTINFVGRSGKNNGVTASV
                     DLLKNTKPYPAGLLTVALGGSVLSTFLQNKPFYTAQNVAVLNPKTEMTEQQKLFYCAA
                     IFANAYRFSACGREANRTLRQLFVPSLDEIPSWVESVNLNPSAGVTEPKLKEPLDLPV
                     VRQSKRLDEIFTIQNGIAATKLKEFEQRQKDTVVYIRPASTQARTLRSYIARDSVDEK
                     HIFPCHTLFTSTNGEGSHTYSYVSTCEFVANSDVAVLTPIQSDMPIEVKLYYAKCITA
                     NRYLFSYGRKPKGEKLKSIMLPYFDQQEDFDYICRFIHTLLFSNNL"
     gene            complement(77987..78811)
                     /locus_tag="WNA25_000076"
     CDS             complement(77987..78811)
                     /locus_tag="WNA25_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HFA9822955.1"
                     /translation="MFALAASNMILRGDGKANLHQSSCFMTDFQDLIKNPKPETGLKR
                     PNVGFLNPPYAQSKSDAELHELYFVKEMLDMLAEGGTGIAIIPVSCVIAPSKAKSEIV
                     KYHRLKAVMSMPSELFYPVGTVTCIVVFEAHKPHFQTVVIDPDTQEEISTKKACRKTW
                     FGYWRDDGFEKTKHLGRIDLYDRWQGIKARWLEHYLNNEVHTGESVTAFVTDNDEWVA
                     EAYLETDYSKITRADFEQVVREFALFQLLGAEVGPTENLDNESYEDDDNNDFGDDE"
     gene            complement(78841..79482)
                     /locus_tag="WNA25_000077"
                     /pseudo
     CDS             complement(78841..79482)
                     /locus_tag="WNA25_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711434.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(79651..79971)
                     /locus_tag="WNA25_000078"
     CDS             complement(79651..79971)
                     /locus_tag="WNA25_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HFA9822956.1"
                     /translation="MNFQDYLATFPSIDHLGGLDVQDAEGKTVHHIPAVQGKLGSLKL
                     YNALAERFDGKLGKEAAEQGLIWFAEHVADARAHPGKHPNIDLLENVVQSGETLLLKP
                     LAAQ"
     gene            complement(79994..80620)
                     /gene="upp"
                     /locus_tag="WNA25_000079"
     CDS             complement(79994..80620)
                     /gene="upp"
                     /locus_tag="WNA25_000079"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFA9822957.1"
                     /translation="MNVNVINHPLVRHKLTLMREADCSTYKFRTLTTELARLMAYEAS
                     RDFEIEKYLIDGWCGQIEGDRIKGKTLTVVPILRAGLGMLDGVLDLIPTAKISVVGLQ
                     RDEETLKPISYFEKFVDSMDERPALIIDPMLATGGSMVATIDLLKEKGCRNIKALVLV
                     AAPEGVKAVNDAHPDVTIYTAALDSRLNENGYIIPGLGDAGDKIFGTR"
     gene            complement(80726..81037)
                     /gene="grxD"
                     /locus_tag="WNA25_000080"
     CDS             complement(80726..81037)
                     /gene="grxD"
                     /locus_tag="WNA25_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HFA9822958.1"
                     /translation="MASIHDQIKEVVTTHRVVLFMKGTKQFPQCGFSSRAVQILNAAG
                     CTDYVAVNVLENPEVRQGIKEYSDWPTIPQLYVNGEFVGGSDILMEMYEAGELQELLK
                     A"
     gene            complement(81138..81353)
                     /locus_tag="WNA25_000081"
     CDS             complement(81138..81353)
                     /locus_tag="WNA25_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFA9822959.1"
                     /translation="MKAIHPYACPRCCRLPANTFRTGMANSASKFCIAKGGRREVKKD
                     ESGGGYALCHLPDSRIVEEWEYCRSQH"
     gene            complement(81379..82158)
                     /locus_tag="WNA25_000082"
     CDS             complement(81379..82158)
                     /locus_tag="WNA25_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFA9822960.1"
                     /translation="MHIIDSHCHLNFEGLKERLPEVLSNMEANGVGQALAISVSRESF
                     SEVFAVAEAHEHIYCTIGVHPDSKEAEEFSIAEMAEAAAHPKVVGIGETGLDYYWCKG
                     DLSWQHKRFADHIEAANQTGLPVIVHTRDAAADTLAILKECKTNSGVIHCFSEDVGFA
                     RAAMDLGLYISFSGIVTFKNAPLVQEAAKYVPDDRILVETDAPFLAPVPKRGRQNEPA
                     FVRHTAEHIAKLRNQTLEQVAAYTTENFYRLFKKVPDMRVV"
     gene            complement(82163..82513)
                     /locus_tag="WNA25_000083"
     CDS             complement(82163..82513)
                     /locus_tag="WNA25_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilZ"
                     /protein_id="HFA9822961.1"
                     /translation="MSDGQNIPAKMMSLQLKDMNLLYSSYMPFLEHGGLFVQTDDVFS
                     IGDDILLAVEILNFPKLFLPTKVAWINPASTSSKPKGVGLAFTKHENCLKVKDQIEVE
                     LGSTISGSRPTFTM"
     gene            complement(82517..83494)
                     /locus_tag="WNA25_000084"
     CDS             complement(82517..83494)
                     /locus_tag="WNA25_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFA9822962.1"
                     /translation="MIYPWHQEQWRQIAEHWTSRPNAWLFVGKKGTGKTAFARFAAKA
                     LLCETPAPGCKPCGECMSCHLFGRGSHPDFYEITPLADEPENGRKLLRIKIDAVREII
                     DNVYLTSVRGGLRVILIHPAESMNVQAANSLLKVLEEPPPQVVFLLVSHAADKVLPTI
                     KSRCRKKVLPAPSHGEALAYLRDRGVAEPEERLAFHSGAPLFQEEGELRELRAKLLEI
                     LAEPRLLKILDYAALFDKEKLPLAVFVGWMQKWLVDLGLCLQHMKPVYYPAYEDRLLQ
                     TASGFRPRNVFAAEDMLKQLAPYGFHTLNVKMQIEHLLINYLELKKENR"
     gene            complement(83528..84640)
                     /locus_tag="WNA25_000085"
     CDS             complement(83528..84640)
                     /locus_tag="WNA25_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HFA9822963.1"
                     /translation="MTAKEELFAWLRHMNKNKGSDLFVTTHFPPAMKLDGKITRITDE
                     PLTAEKCMEIAFSIMSAKQAEEFSSTNECNFAISLPDTSRFRVNAMIQRGATALVFRA
                     ITSKIPKFESLNLPPALKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNENSFGHII
                     TIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDRETMDYA
                     IAFAETGHLCMATLHANSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQRLVPRD
                     GGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKSTTLGMQTFDQHLYQLYEKGEI
                     SLQDALKNADSAHDLRLAVQLRSRRAQSSDPDLELL"
     gene            84826..85527
                     /locus_tag="WNA25_000086"
     CDS             84826..85527
                     /locus_tag="WNA25_000086"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFA9822964.1"
                     /translation="MSLKTVAVIGAMEQEIELLREMMENVKAVSFGRFSAYEGELAGK
                     RMVLALSGIGKVNAAVATAWLIRQFAPDCVINTGSAGGLGKGLKVGDVVIGTETEHHD
                     VDVTAFGYARGQVPQLPARFASDGILIETAKRAARTFEGAEVEQGLIVSGDRFVHSSE
                     GVAEIRKHFPEVKAVEMEAAAIAQTCHQLETPFVIIRAVSDSADEKADISFEEFLKTA
                     AASSAKMVAEIVKSL"
     gene            85669..87462
                     /gene="lepA"
                     /locus_tag="WNA25_000087"
     CDS             85669..87462
                     /gene="lepA"
                     /locus_tag="WNA25_000087"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HFA9822965.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLADRFIQYCGGLDLREMSTQVLDSM
                     DIEKERGITIKAQTAALNYKARDGQVYQLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTAIDLGVEVVPVLNKIDLPAADPERVEQEIEDIIGIDAVGAVQ
                     CSAKSGIGVEDVLEEIVAKIPAPTGDENAPLQAVIVDSWFDNYVGVVMLIRVKNGTIK
                     LKDKVRFMSTKAETQVEQLGVFTPKSVQKQELKAGEVGFLITGVKELGQAKVGDTVTL
                     VANPATEPLPGFQEVQSQVFAGLYPVESHDYEALRDALEKLQLNDASLKFEPEVSQAL
                     GFGFRCGFLGLLHLEIVQERLEREFDMDLITTAPTVVYEVVLKSGEKIEVENPSKLPD
                     IGSIETILEPIITATILVPQEYVGNVMTLCNQKRGVQVNMQYMGRQVMLTYDLPMNEV
                     VMDFFDKLKSTSRGYASLDYHFKEFQPSDLIKLDIMVNGEKVDALSLIVHRQSAVHKG
                     RELASKMRELIPRQMFDIAVQAAIGSRIIARENVKALRKNVLAKCYGGDITRKKKLLE
                     KQKAGKRRMKQVGNVEIPQSAFLAILQVSDK"
     gene            87608..88627
                     /gene="lepB"
                     /locus_tag="WNA25_000088"
     CDS             87608..88627
                     /gene="lepB"
                     /locus_tag="WNA25_000088"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFA9822966.1"
                     /translation="MNTMLMSGAAAALLAGIILYFKSDKKRQENGEWSSGLEYAYILT
                     AVGVFAALSLFMSFTAVFLIFVVLCGTAWGVYKYRLKTHPEISESSHFGDYFGSFFPT
                     VLVLFLIRSFIAEPFQIPSSSMRPGLIKGDFILVGKFSYGLRVPVLNNVFIPTGKIER
                     GDVVVFNYPLQPEMTYIKRIVGIPGDIVEYRDKVLTVNGKPASDIPDGTYRYPDDTDP
                     SEIHNTDMFRSGLDGKSFNILKKEGQPAVSLPVLGKYTSDIMSENGYSIDQSGLNHCQ
                     YADDGSGFVCKVPEGRYFAMGDNRDNSADSRYWGFVDDKLVVGKAMFILMNFGDFGRA
                     GTAIR"
     gene            complement(88732..88839)
                     /locus_tag="WNA25_000089"
                     /pseudo
     CDS             complement(88732..88839)
                     /locus_tag="WNA25_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(89063..89212)
                     /locus_tag="WNA25_000090"
     CDS             complement(89063..89212)
                     /locus_tag="WNA25_000090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9822967.1"
                     /translation="MDWQAVKTGKTKHADNGAERKMPSEALSDGIGAGRIKKAASAGM
                     AMQLK"
     gene            complement(89258..90165)
                     /locus_tag="WNA25_000091"
                     /pseudo
     CDS             complement(89258..90165)
                     /locus_tag="WNA25_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273746.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
     gene            complement(90474..91406)
                     /gene="cysK"
                     /locus_tag="WNA25_000092"
     CDS             complement(90474..91406)
                     /gene="cysK"
                     /locus_tag="WNA25_000092"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFA9822968.1"
                     /translation="MKIANSITELIGNTPLVKLNRLTKGLKAEVAVKLEFFNPGSSVK
                     DRIAEAMIEAAEKAGKINKNTVIVEATSGNTGIGLAMVCAARGYKLAITMPESMSKER
                     KMLLRTFGAELILTPAAEGMAGAIAKAQSLVDAHPDTYFMPRQFDNEANPEVHRKTTA
                     EEIWNDTDGKVDVFVAGVGTGGTITGVGEVLKKYKPEVKVVAVEPEASPVLSGGEKGP
                     HPIQGIGAGFIPTVLNTKIYDSIAKVPNEAAFETARAMAEKEGILAGISSGAAVWSAL
                     QLAKQPENEGKLIVVLLPSYGERYLSTPLFADLA"
     gene            complement(91602..92207)
                     /locus_tag="WNA25_000093"
     CDS             complement(91602..92207)
                     /locus_tag="WNA25_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9822969.1"
                     /translation="MILLHLDFLSALLYAAVFLFLIFRAGMLQWFWASIALWLGISVL
                     GVKLMPGMWGMTRAAPLFIPHFYLTLGSIFFFIGYWNRKTDGNGWQADPEHPLLGLFA
                     VSNVSMTLAFVGICALVHYCFSGTVQVFVFAALLKLYALKPVYWFVLQFVLMAVAYVH
                     RCGIDRQPPSTFGGSQLRLGVLAAMLMQVAVTAMLLAEIGR"
     gene            complement(92204..93055)
                     /gene="dapF"
                     /locus_tag="WNA25_000094"
     CDS             complement(92204..93055)
                     /gene="dapF"
                     /locus_tag="WNA25_000094"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFA9822970.1"
                     /translation="MKTLKFTKMHGLGNDFMVIDAVSQDFTPEDVPIAAWADRFRGVG
                     FDQLLVVGCSETEGVDFRYRIFNADGSEVGQCGNGARCFARFVADKGLTDKKEICVET
                     AKGIIFPKLSDNGMVTVNMGKPKFMPSEIPFVPESGEGDDACIYGVHLESGIQPVSCV
                     NMGNPHAVIVVDDVECAPVRETGSRIEPHRQFPERVNVGFMQVVGRTAIRLRVFERGV
                     GETQACGTGACAAVVAGIRLGLLDEGKTVEVVLPGGTLYIEWACGGDVMMTGPAETVF
                     EGELAYS"
     gene            93192..93506
                     /locus_tag="WNA25_000095"
     CDS             93192..93506
                     /locus_tag="WNA25_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9822971.1"
                     /translation="MSRLFLLLFPWATLAACTRNIPASPAVSNCAHLHNRTVLYFDAH
                     GKPADSTAKGGFYRTVEIHDTNRILIQDFNSTGNALVKPYWSDCRKLSVFPDKHLTMS
                     HP"
     gene            93503..94171
                     /locus_tag="WNA25_000096"
     CDS             93503..94171
                     /locus_tag="WNA25_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFA9822972.1"
                     /translation="MNHDITFLTLFLLGFFGGTHCIGMCGGLSSAFALQLPPHINRFW
                     LILLLNTGRISSYTAIGLMLGLIGQLGISLDQTRVLQNILYTASNLLLLFLGLYLSGI
                     SSLAAKIEKIGKPIWRNLNPILNRLLPIKSIPACLAVGILWGWLPCGLVYSASLYALG
                     SGSATTGGLYMLAFALGTLPNLLAIGIFSLQLKKIMQNRYIRLCTGLSVSLWALWKLA
                     VLWL"
     gene            complement(94244..96367)
                     /gene="pnp"
                     /locus_tag="WNA25_000097"
     CDS             complement(94244..96367)
                     /gene="pnp"
                     /locus_tag="WNA25_000097"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFA9822973.1"
                     /translation="MMFNKYVKTFQYGNQTVTLETGEIARQAAAAVKVSMGDTVVFVA
                     VTTNKEVKEGQDFFPLAVDYLERTYAAGKIPGGFFKREGKQSEKEILTSRLIDRPIRP
                     LFPEGFYHDIQIVAMVVSVDPEIDSDIPAMLGASAALVLSGVPFAGPIGAARVGYING
                     VYVLNPTKAELAKSQLDLVVAGTSKAVLMVESEAKILPEDVMLGAVVYGHDQMQVAIN
                     AINEFADEVNPEVWDWKAPETNEELVAKVRGIAGETIKEAFKIRQKQARSAKLDEAWN
                     AVKEALITEETDTLAANEIKGIFKHLEADVVRSQILDGQPRIDGRDTRTVRPLNIQTG
                     VLPRTHGSALFTRGETQALAVATLGTSRDEQIIDALSGEYTDRFMLHYNFPPYSTGEV
                     GRMGAPKRREIGHGRLAKRALLAVLPKPEDFSYTMRVVSEITESNGSSSMASVCGGCL
                     SLLSAGVPLKAHVAGIAMGLILEGNKFAVLTDILGDEDHLGDMDFKVAGTTEGVTALQ
                     MDIKIQGITKEIMQIALAQAKEARLHILDQMKAAVAGPQELSAHAPRLFTMKISQDKI
                     RDVIGKGGETIRSITAETGTEINIAEDGTITIAATTQEAGDAAKKRIEEITAEVEVGK
                     VYEGTVVKILDNNVGAIVSVMPGKDGLVHISQIAHERVRNVGDYLQVGQVVNVKALEV
                     DDRGRVRLSIKALLDAPVREENAAE"
     gene            complement(96601..97464)
                     /locus_tag="WNA25_000098"
     CDS             complement(96601..97464)
                     /locus_tag="WNA25_000098"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFA9822974.1"
                     /translation="MSEIGLVKIYSGKVRDLYEIDDKRMLMVASDRLSAFDVILDAPI
                     PGKGEILTQISNFWFKKLAHIMPNHFTGDTVYDVLPENEAKALEKRAVVAKKLTPVKV
                     EAIVRGYLAGSGWKDYQKTGSVCGIRLPEGMREAQQLPEVIFTPSTKAAVGDHDENIS
                     FEECGRIIGKELAEEVRDKAVRLYTEAAEYAKSRGIIICDTKFEFGLDEKGTLTLMDE
                     VLTPDSSRFWPADQYEVGTNPPSFDKQFVRDWLEQSGWNKKAPAPKVPADVIRKTVEK
                     YQEALTLLTQD"
     gene            97657..98520
                     /gene="rfbD"
                     /locus_tag="WNA25_000099"
     CDS             97657..98520
                     /gene="rfbD"
                     /locus_tag="WNA25_000099"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFA9822975.1"
                     /translation="MRILLTGSKSQLAHCLRDRLPEDWETIATDSASLDITDADAVCN
                     MVKSFQPDAIVNTAAYTAVDKAEGDAAAAFAVNASAVYNLALAAHRAHARFIHISTDY
                     VFDGKGKIPYQESDFTNPSNVYGQSKTAGELLALSANPDSLILRTSWLFSEYGDNFVR
                     TMLNLARERSPLSAVHNQIGCPTYAGDLSAAIIRLLQQSNPVRGIYHYAGGKSVSWYE
                     FARHIFQTALQQDASFPVPELKAVSDEGSAAAAPRPAYSILDCRKIENDFGIKPSDWQ
                     KALAQVVSKLL"
     gene            complement(98892..99125)
                     /locus_tag="WNA25_000100"
     CDS             complement(98892..99125)
                     /locus_tag="WNA25_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HFA9822976.1"
                     /translation="MKLTLMFREYCSLCHKMRDALKPFQDEYGFGLEVVDVDENPVLE
                     EKYNELIPVLLAGDEEICHWFLDEDRLKQFLER"
     gene            complement(99291..99491)
                     /locus_tag="WNA25_000101"
     CDS             complement(99291..99491)
                     /locus_tag="WNA25_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFA9822977.1"
                     /translation="MFVCICNAVTDHQIKETIAAGATTMGDLQSQLGVASCCGCCGEL
                     AASFLTAHNAQPTVTAGINVQA"
     gene            complement(99663..100538)
                     /gene="xerD"
                     /locus_tag="WNA25_000102"
     CDS             complement(99663..100538)
                     /gene="xerD"
                     /locus_tag="WNA25_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFA9822978.1"
                     /translation="MENGLIDRLLETLWLDRRLSRNTLDSYRRDLEKIARRLSLCGRT
                     LKDADEADLAAAVYVDGEQRSSQARALSACKRLYVWMEREGMRADNPTRLLKPPKIDR
                     NIPTLITEQQISRLLAAPDTDTPHGLRDKALLELMYATGLRVSEAVGLNFGNVDLDRG
                     CITTLGKGDKQRMVPMGQESAYWVERYYTEARPLLLKGRSCDALFVSQKKTGISRQLA
                     WMIVKEYAGRAGIGHISPHSLRHAFATHLVRHGLDLRVVQDMLGHADLNTTQIYTHVA
                     NVRLHSVVKEHHSRN"
     gene            complement(100590..101030)
                     /locus_tag="WNA25_000103"
     CDS             complement(100590..101030)
                     /locus_tag="WNA25_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HFA9822979.1"
                     /translation="MDMKYEFTLPSSSGADFHSAEHLPLVVYFYPKDSTPGCTTEGLD
                     FNARLEQFEALGYTVVGISRDGVKAHQNFCAKQGFRFELLSDKDETVCRLFDVIKLKK
                     LYGKESLGIERSTFVLNKDGEIVREWRKVKVAGHAQEVLETLSR"
     gene            101351..102289
                     /locus_tag="WNA25_000104"
     CDS             101351..102289
                     /locus_tag="WNA25_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFA9822980.1"
                     /translation="MSIRTLKRLPSSLLLGLCLSLPSAHLFADNDILGQFLEQNMLTS
                     SDPIEIFAESTIYPADTQAITGGLILSSQSALVVNNKTGQILYQKNADRIMPIASISK
                     LMSAMVVLDANLDMNETVTITPDEIDRLKGTGSRLAIGTALTRKELLHLSLMSSENRA
                     THALGRTYPGGMGAFVAAMNRKAQSLGMYGSRFYEPTGLNFQNVSTAKDLSLMVNAAA
                     QYPQIRTNSTSNYASVQTKNGQQNYKNSNALVREGMWNIELQKTGYIREAGRSMVVKA
                     NIQNQPVTIVLLNSPTSATRVNDARKIESWMLQQRS"
     gene            102418..102711
                     /gene="hfq"
                     /locus_tag="WNA25_000105"
     CDS             102418..102711
                     /gene="hfq"
                     /locus_tag="WNA25_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFA9822981.1"
                     /translation="MTAKGQMLQDPFLNALRKEHVPVSIYLVNGIKLQGQVESFDQYV
                     VLLRNTSVTQMVYKHAISTIVPARSVNLQHENKPQAAPASTLVQVETVQQPAE"
     gene            102796..103362
                     /locus_tag="WNA25_000106"
                     /pseudo
     CDS             102796..103362
                     /locus_tag="WNA25_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237812.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            103416..103790
                     /locus_tag="WNA25_000107"
     CDS             103416..103790
                     /locus_tag="WNA25_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HFA9822982.1"
                     /translation="MQYLIVKYSHQIFVTITILVFNIRFFLLWKNPEKPLVGFWKALP
                     HLNDTMLLFTGLWLMKITHFSPFNAPWLGTKILLLFAYIALGMVMMRARPRSTKFYTV
                     YLLAMCCIACIVYLAKTKVLPF"
     gene            103800..104294
                     /gene="folK"
                     /locus_tag="WNA25_000108"
     CDS             103800..104294
                     /gene="folK"
                     /locus_tag="WNA25_000108"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFA9822983.1"
                     /translation="MNNRHFAVIALGSNLDNPAQQIRGALDALSSHPDIRLEQASSLY
                     MTAPVGYDNQPDFINAVCTVSTTLDGIALLAELNRIEADFGRERSFRNAPRTLDLDII
                     DFDGISSDDPRLTLPHPRAHERSFVIRPLAEILPDFILGKYGKVVELSKRLGNQGIRL
                     LPDR"
     gene            complement(104422..104901)
                     /locus_tag="WNA25_000109"
     CDS             complement(104422..104901)
                     /locus_tag="WNA25_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HFA9822984.1"
                     /translation="MPESIFKQISSDILRLHRDSVYSLLATSGCNCQVHEAAYVNIDG
                     KYYIALSCEPEVGEVETGILLIEDESRNLRLSWVGSARELDRKDNAYKRALSALSRKL
                     GRCKDKLHTAVQPFLLELVPEKGRFSVGDEEVWISRNDLVRALYPVGYSMRQAVLQI"
     gene            complement(105121..105858)
                     /gene="ubiE"
                     /locus_tag="WNA25_000110"
     CDS             complement(105121..105858)
                     /gene="ubiE"
                     /locus_tag="WNA25_000110"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFA9822985.1"
                     /translation="MGGQKTHFGFSTVNEDEKAGKVAEVFHSVAKNYDIMNDVMSAGL
                     HRVWKHFTIHTAHLKKGDKVLDIAGGTGDLSRGWAKRVGKEGEVWLTDINSSMLTVGR
                     DRLLNEGMILPVSLADAEKLPFPDNYFNLVSVAFGLRNMTYKDAALKEMYRVLKPGGT
                     LLVLEFSKIYKPLEGAYDFYSFKLLPVMGRLIAKDADSYQYLAESIRMHPDQETLKQM
                     MLDAGFDSVDYHNMSAGIVALHKGVKF"
     gene            complement(105901..106269)
                     /locus_tag="WNA25_000111"
     CDS             complement(105901..106269)
                     /locus_tag="WNA25_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HFA9822986.1"
                     /translation="MNNIPVEIRLLKNRAVLVLTYGDEPKNLPAEFLRVYSPSAEVRG
                     HGVGQDVLQTGKADVQITDLQPVGQYALKISFSDGHDSGLYDWAYLHRLAYGYDAMWQ
                     EYLDKLAAAGASRFEKQSDC"
     gene            106389..108008
                     /gene="mnmC"
                     /locus_tag="WNA25_000112"
     CDS             106389..108008
                     /gene="mnmC"
                     /locus_tag="WNA25_000112"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFA9822987.1"
                     /translation="MDNLVWDGIPDIRTLDQTIRKHAHPLNLIVCLPDNQIPDFQTAQ
                     DASDSECRLKHRLDQATQCLQFDSINLIEHILPDVRFWLVPPSRTRRLHEHFHHISWQ
                     TEAIPQTESKSDKPWFALPQTSERKKPEHVLVIGAGIAGASTAHALASHGISVTVLEA
                     RKAAQAASGNRQGLLYAKISPHDTGQTELLLAGYGYTKRLLGHILPDSDTWGGNGIIH
                     LNYSRTEQQRNHELGLQKHHNHLYRSITSAEAEKIAGIPLNTPYAEPLCGLYWQHGVW
                     LNPPAFVRTLLSHPLIELYENTTLTGISHDGEKWIASTPNGTFTATHIIYCTGAHSPC
                     LPETNLAALPLRQIRGQTGLTPSTPFSEQLRCAVSGESYISPSWHGLHCYGASFIPNS
                     SNTGWNEAEEASNRQALAHLNPALAESLFAANPNPQKHQGHAAIRCDSPDHLPVVGAL
                     GDIAAMRQTYAKLALDKNYRIDTTCPYLPNAYTNTAHGTRGLATAPICAAAVAAEILG
                     LPHLFSQRLRHALHPNRTVIRAIVRRQNLIP"
BASE COUNT        26404 a        29911 c        26393 g        25648 t
ORIGIN      
        1 aaagacggca aaaatcccgc actcatcgct ttcctcgaaa gcacggttta cgaaaaatcg
       61 cgcaacaaag cctcgttaat caaaaaaagg gggctttgcc ccttttataa cttaagggta
      121 aattatgaat attaaaaatg aaatagttgc tttaatttct aaaaaaattg atgggggggc
      181 aatatagttc tttagaaaat tttgctttat ccgtactaag ggaaaaaatt ataaacaata
      241 ctgtttccag tgatgaaaaa ttattgatta ttaatgattt tttatcaggt tttcttgagt
      301 ttgatcctga gacaggggaa cctgtcgggg agacgcttaa aattgagcaa atgattgatt
      361 tctttttagt ctaaatcata cataccagtg gacaagctag gcgtagatcc ggcttttaaa
      421 tacgattgtc aggtttatac ttgggataaa atcaaaaata aagttgttta aactatagtg
      481 gattaacaaa aatcagggca aggcgacgaa gccgcagaca gtacagatag tacggaaccg
      541 attcactcgg tgcttcagca ccttagggaa tcgttccctt tgagccgggg cggggcaacg
      601 ccgtactggt ttttgttaat tcactatatt gcatggtgca tgcattaaat cagtcttcta
      661 gcgccgcaat gggaaaacat agaagataaa cgaaatggat gatctaatac tatatttttt
      721 atcaggtata ttcggaaatc aagttgctga atatattata aaaaacaacc gcgagataaa
      781 agttcccttt attgcgcttt acgctatatt ttttacgttg atttatactg tggctttgtt
      841 gtttctcagt ctgatctatt gggtcaatgg tgcggagatt gcatggaagg ggataggtat
      901 tttcagtatg tcggtcagtt tttgtatagt cttctgtctt tatttggttg acaaggcagg
      961 aagatgtaag gataagaaac aatagggatc agatgcggca gcaaacgtag attgggtcta
     1021 ggctcaacct gcgtttgctg catctttgga agaaatagtg gattcgaaaa aataggaaga
     1081 attctgatta atggtatgcg aggtgtagca gtgggggctg ttgctggtgg cataactggt
     1141 tatgcaggtt ctacgggtaa gaacactgac attcgccgtt aaataataaa taaaccaact
     1201 tccaaccaaa cctgctatcg gtttggttgg aagtgagagg aggtaaggta gtgtttgctg
     1261 ttattttctt ttcgacattg ggctgtattt tagcatggat aagagatatt cctaaaataa
     1321 aatcaaaaaa aattttggca agatccttat atattatagg aataataaat gtaataatca
     1381 gctatgtatt aattaaaaat atattggttt ctgtttcgga tggaggtgga ataaaatatg
     1441 ttgcaatata tttatcaaat cttttttttg gacagtattg atgtatgctc ttgtgaaaag
     1501 attatctaaa aagccaagct gaaacccagt acacaacaca tcaataaaga ttccttttgc
     1561 acggaacaat gacaatattc aaattgctgg atttgcaatc tgatttacct attgaaattg
     1621 ggtgtgcaac atacgttcta agcaggcttt gatttcccaa agaggctgaa gggtaagaga
     1681 cgcttatatc atagataaat aatggcaaaa caggtaatga attaaaggca ttacctgttt
     1741 ttacagaaat ttttaaaata gatgatttga ttgcagcttt ttaggctatt tatcaggtaa
     1801 atacagtgaa gggaaaataa gcaggttaga aatatcccta acggcaggaa gcattttctt
     1861 tgcagtattc tttattcatg aactgataat ttttattgtt gatggaaata aggaaaatct
     1921 ttggaatttt attactggga taatgaaaga tttattttta ttttcatgtt ctgtttgttt
     1981 attattctat ttcttattat tgattgcaga aaccattgga aaaacaatca ataagattaa
     2041 ataagatatt taagccgcta aaaaaccatt tgagtatttt atcttctatg cggcatccat
     2101 cttggaattg aaatactgca atcgggaggc taaggcttcc gttgtaacgt ttaccatata
     2161 tttcattatt ttaatttcat caaaaaacaa aattcaagat tatacatacc gccaatcgtt
     2221 tcttgattgt tttgtatagt ggagtaaatt taaatcagga caaggcgacg aagccgcaga
     2281 cagtacaaat agtacggaac cgattcactc ggtgcttcag caccttagag aatcgttctc
     2341 tttgagctaa ggcgaggtaa tgccgtaccg gtttaaattt aatccactat atatttatcc
     2401 gatatatcaa catctctcta tcttattccg aattttattg ttgaaacttg cattaaaaac
     2461 catacaagat gatgaagaac aaatatattt taataaaagc agcaatcggc ataaccgcca
     2521 tatccatatt ttatcttctg actcaaggaa gcatcggcaa aactgaagaa ccatcatatt
     2581 tcttgatgtt catgtttctc aactcgcttt ggtttgaaga aaataaaaca gttatggctg
     2641 ccgttacggc aatgattgcc gcccacttta tatttgtcgc actatccgat taataagcaa
     2701 acaaaaaggc tgcttggaaa tttcaggcag cctttcagga ggattttaat ggaacaaaaa
     2761 aggcggtttg ccgcctccct gctcctcgcc gccgccctgc cgctttgtgc acattctttt
     2821 ccctttgcgg aagaaaaccc catagcctac ggcaaagtca aaatacagag ttggaaagcg
     2881 cggcgggatt tcaatattgt aaagcaggat ttggattttt cctgcggggc ggcttcggtg
     2941 gcgacacttt tgaacaattt ttacgggcaa aagctgacgg aagaagaagt gttggaaaaa
     3001 ctgggtaagg aacagatgcg cgcgtcgttt gaggatatgc ggcgcattat gcccgatttg
     3061 ggttttgagg cgaagggcta tgccctgtct ttcgagcagc tcgcgcagtt gaaaatcccc
     3121 gtcatcgtgt atctgaaata ccgcaaagac gaccattttt cggtattgcg cggagtggat
     3181 ggcaatacgg ttttgcttgc cgacccgtcg ccgggtcatg tttcgatgag cagggcgcag
     3241 tttttggagg cttggcaaac ccgtgaggga aatttggcag gcaagatttt ggcggtcgtg
     3301 ccgaaaaaag cggaggcgat ttcaaataaa ttgtttttca cacatcatcc caagcggcag
     3361 acggagtttg cagtcggaca aatcaggcaa gggcgcgcag agtaagtcaa atgccgtctg
     3421 aaaactgttt cagacggcat ttttacccgt ccgcaaacca tctccatcct cacaagccct
     3481 taaaaatccg ctataatcgc ccgcaatttg atttcagcac cccctttttg aaatggcaca
     3541 aaaaatccaa tccgtcaaag gcatgaacga ccttctgcct gtcgggcaaa aagatttcaa
     3601 actcacggct gtgttttggc aggcgtttga ggatacggta aaccgttgga cgcgagctta
     3661 cggttatcag caaatccgta cgccgattgt cgagcaaacc ggtttgtttg tccgctccat
     3721 cggcgaggaa accgatgtag tcggcaagga aatgtacacc ttctccgatt caaacgattc
     3781 tttgagtttg agcctgcgtc ccgaaggtac ggcctcttgt ctgcgtgcgg tggtcgaaca
     3841 caaccttctg tacaacagcc cgcaaaagct gtggtatatg gggccgatgt tccgccgcga
     3901 gcgtccgcaa aaaggacgct accgccaatt tcatcaggtc ggtatcgagg ctttgggttt
     3961 tgaagggccg gatattgatg cggaaatcat cgcaatgtct gccgacttgt gggaaaaatt
     4021 gggtattcgc gaatacctga ctttggaaat caacagcttg ggcaaccgtg aggaacgcgc
     4081 ggcacaccgt gcggcattgg ttgaatatct gacccgctat gaagcgcaac tggacgaaga
     4141 cagcaaacgc cgtctgaaaa ccaatccttt gcgcgttttg gatacgaaaa accctgattt
     4201 gcaggaaatc tgcaatgccg cgccgcgttt ggtagattac ttgggcgagg cttcgcaaaa
     4261 ccactatgca cgcttcaagg cgatgttgga cggtttgggt attcaatata ttgaaaattc
     4321 gcgcttggtt cgcggtttgg attattacaa tcagacggtt tttgagtgga cgaccgacaa
     4381 actcggcgca caggcgactg tgtgcggcgg cggacgttac gacggtttga ttgaagaact
     4441 cggcggcaag cctgcgccgt ccatcggctt tgcgatgggc atcgaacggc tgctgctttt
     4501 ggtgagcgaa tacggttcgc tggaagtgaa cgccgcgcct gatgtctatg caatgcacca
     4561 aggcgaaggg gcggacttgc aggtgatgaa atacgcacaa gccttgcgcg cgcaaggttt
     4621 caatgtaatg cagcattccg gctatcaaag cctgaaggcg caaatgaaaa aagccgacaa
     4681 cagcggcgcg cgctttgccc tgattgtcgc gcaagacgaa ctggcggacg gtaccgttac
     4741 gctcaaagac atgaacggcg cacacggtca gcaaaccgtt tccgccaccg atttgacaga
     4801 tactttacaa caatggaaga acgcataaat ggcagcccat ctcgaagaac aacaagagtt
     4861 agacaacttt aaatattttt ggaaaaccac gggcaaatgg ctgtttgccc tgctgatttt
     4921 ggcggcactc ggctacttgg gatacacggt ttaccaaaac cgtgcggctt cccaaaatca
     4981 ggaagcggcg gcggtgctgg caaacatcgt ggaaaaggcg caaaacaaag ccccgcaaag
     5041 cgaaatcaat gccgaactgt ccaagctcca acaaagctac ccccattcca tttccgccgc
     5101 ccaagccacg ctgatggcgg cggcaaccga atttgacgcg cagcgttacg atgttgccga
     5161 aggtcatttg aaatgggtgt tgtccaacca aaaagacagc ctgattcagg cgttggcggc
     5221 gcagcgtctg ggcgttgtgt tgttgcaaca aaaaaaatac gatgccgcgc ttgccgcact
     5281 cgacacgccg gttgaggcgg acttcgcccc cctgctgatg gaaactaaag gcgatgttta
     5341 tgccgcacag gaaaaaagcc aggaagcctt aaaaaactac ggacaggctt tggaaaaaat
     5401 gcctcaagat tctgtcggtc gcgaattgct tcaaatgaaa ctcgattcgc tgaaataaat
     5461 accgtctgaa atgccgctct ctcttcagac ggcatttcag acaaccccat cctacggcaa
     5521 ttcccgtaaa accatccgcc gtgtcccaac ggacacctgc tcgtgttcag ttctaccgaa
     5581 cggcacttga cacagaaaga cccaatcatg aaaccaacca tcgcgcttat cggccgcccc
     5641 aacgtcggca aatctacctt gttcaaccgt ttgacgcgca ccaaagacgc gctcgtgcat
     5701 gacctgccgg gtctgacccg cgaccgccat tacggacacg gcaaagtcgg cagcaaacct
     5761 tattttgtca tcgataccgg cggtttcgag ccggttgtgg acagcggcat tttgcacgaa
     5821 atggcaaaac aaaccttgca ggctgtcgat gaagccgatg cggttgtgtt tttggtggac
     5881 ggccgtaccg gtttaacacc gcaagacaag attattgccg accgtttgcg ccaaagtccg
     5941 cgtcccgttt atttggccgt gaataaaggc gagggcggcg acagggccgt acttgccgcc
     6001 gagttttacg agctggcatt gggcgagccg cacgttattt ccggcgcgca cggcgacggc
     6061 gtgtattacc tgattgaaga aattttagaa aacttccccg agcctgaagc cgaagaagcc
     6121 gatgcaaaac atcccgtttt tgccgttatc ggtcgtccga acgtcggcaa atctacgctg
     6181 gttaacgcca ttctcggcga aaaacgcgtc atcgccttcg atatggcagg cacaacgcgc
     6241 gacagcatcc acatcgattt cgagcgcgaa ggcaaaccgt ttaccatcat cgataccgca
     6301 ggcgtgcgcc gtcgcggcaa agtggatgaa gcggtggaaa agttctccgt tatcaaagct
     6361 atgcaggcgg ttgaagcggc aaacgttgct gttttggtat tggacgcgca acaggacatt
     6421 gccgaccaag atgcgacgat tgccggtttt gctttggaag cgggacgcgc gctggtagtt
     6481 gccgtcaaca aatgggacgg catcagcgaa gagcggcgcg aacaagtgaa acgcgacatt
     6541 tcccgcaaac tgtatttcct cgattttgcc aagttccact ttatttccgc gttgaaagag
     6601 cgcggtatag acggtttgtt tgaaagcatt caagctgcct acaacgcggc gatgattaag
     6661 atgccgacgc cgaaaatcac gcgcgtattg caaaccgccg tcggacgcca acagccgccg
     6721 cgtgccggct tggtgcgtcc gaaaatgcgt tatgcccacc aaggcggcat gaatccgccg
     6781 gtgattgtgg tacacggcaa ttcgctgcac gcgatttccg acagctatac gcgctatctg
     6841 acccagacct tccgcaaagc cttcaacctg caaggcacgc cgttgcggat tcaatacaat
     6901 gtttcggaaa acccgtatga aaatgcggaa gacaaaccga agaaaaaacc gctgcgccgc
     6961 gtcagcctga gcaaccgtat tgagaaacgc gaaggccgaa aggaagagaa aaaccgcttc
     7021 aagaagaaaa ccaaagtcag tgtgaaaaaa caattcagca aataattccc gacatttcaa
     7081 acaataggaa acattatgtg gttcaagcag attagttttt atccgctcaa caaagaaaag
     7141 ctgcctgagg cggacgtact tgccgataaa cttgctgaag ctgaatttac ccattgccaa
     7201 ggtttggact ggttcagcga aggctttacc gcaccggttt ctttttcgcc cgaactcgtt
     7261 ttccctgccg actttacctt gcgcgtcgcc ctgaaaaaag aggaaaaagt cctgcctgcc
     7321 ggcgtcatcc gcgatatttt ggaagagaag gtagcggaaa tccaaaacaa tgaagcccgc
     7381 aatgtcggtc gcaaagaaaa acaagagctt aaagagcaaa ttacagacga cctgctgccc
     7441 cgagcgttta cccgcagcag ccgtacagaa gcggtgttta acacccgcca cggctacctg
     7501 ctcgtcaata acgcggcttc cgccaaagcg gaaaacatcc tgaccaagct gcgcgaagct
     7561 ttgggaggtt tggaagcctc gctgccgaat accaaacaat cgccctcttc cctgatgacc
     7621 ggctggctgt tgcaagggca ttgcgaaggc ggttttgaat tagacagcga ttgcgaactc
     7681 aaaggtacgg gcgatattgt tcccgtcgtc aaagtatcca aacaagactt aaccgccgac
     7741 gaagtggttc aacacgtcaa aaacggtaaa accgttaccc ggctcggttt ggtttggcgc
     7801 gaacaaatcg cctttatcct cactcaagac ttcacactca agcgcatcca atacctcgac
     7861 gtattgcagg aagaagccga aagcaacggc gacgatgccg ccggccttgc cttcgcctcg
     7921 caaatcctga tggcggaatc cgtcagcacc atgttggaag agctggtttc ctatttgggc
     7981 ggctggcaag attgattttt cagacggctt ttataccgtc agccaaaatc caatggaata
     8041 acccttatgc ttaaacatct cgcattccta ctgcccgcca tgatgttcgc cctccccgcc
     8101 cagaccgccg tcctaagccc ctatcaggaa accggctgca cctacgaagg cgggatcgga
     8161 aaagacgggc ttccttcagg caaaggcata tggcgttgcc gggatgggcg cggttatacc
     8221 ggttcattca aaaacggcaa attcgacggg caaggcgttt ataccgttgc cgccggccgc
     8281 gaagtatttc tcgagccgtt caattccgac agtaccaaat tccgcaatat ggcattgtcg
     8341 ggcacgttca aacaaggctt ggcacacggc aggttcgccg cctcgcaaaa cggcgaaacc
     8401 ctcttttatt atgaaatgcg aacacggcat gattaaagaa gtgaaactgc ccaaaaacaa
     8461 ataacccccc gtttttcaaa cagcatcccc caatgcttta tccgaacacc gcaaaaaaca
     8521 aggaaacccc atgagcatca agtccgacaa atggatacgc cgaatgagcg aagaattcgg
     8581 catgattgat cccttcgagc cgaaccaaat caaagaagcc gacggacagc gcatcatctc
     8641 ctacggtacg tccagctacg gctacgatat ccgctgcgcc aatgaattta aaatttttac
     8701 caacatcaac agcaccatcg tcgatcccaa aaacttcgac ccgaaaaatt tcgttaccgt
     8761 cgaagacgac tgctgcatca tcccgcccaa ttccttcgca ctggcgcgca cggtcgaata
     8821 tttccgcatt ccgcgcaacg tcctgaccgt ctgtttgggc aaatccacct acgcccgctg
     8881 cggcatcatc gtcaacgtta ccccgttcga gccggaatgg gaaggctacg ttaccctcga
     8941 gttttccaac accacccccc tgcccgccaa aatctacgca ggcgaaggcg tggcgcaagt
     9001 cctcttcttc gagagcgacg aagtgtgtga aacttcatac aaagaccgca acggcaaata
     9061 catgggacaa accggcgtaa ccctgcccaa agcctgaaat acaaaatgcc gtctgaaccc
     9121 ttatcgtcgg gtttcagacg gcatctacct atttatccgt ggttagaact gaaccgtccc
     9181 attgatgctg atgctgattt cctccacacc cggtgcggct gaatccgtac ccttcatatt
     9241 gacgcttgcc gccatcggca tcgcgcgcag catttttgcc cgaacagccc catcgcccgc
     9301 aatatggctg ccgatttgcc caaaattcaa tttgacgatt ttataaccgg acgcacccag
     9361 aacgcccgcc agtttttcgg cacgcgcctt gaaacgcaaa acggcatcct tgctgacctg
     9421 atcgatgacc tcgtttcggc gttcgcgcga cacgctgaaa tcggtatctt caagcgaagc
     9481 atccgtctga acatcagcaa taaaacggtt taaagcatca aaatccctgc cctccgcctt
     9541 aaattccgca cgctcctccc agcctgtttg aatgcgtctg ccgttggtat attgatagcg
     9601 cggcatcgca ctgcgcgata ccaattcggt tttaaagcta ccatttttcg attttctggt
     9661 aaaattgttg aattttttaa caaactcggc attgacggca tttttgtccc gtccttccgc
     9721 cgccacctgg aaacgcgcgg acattgtatc ctgagccacc tcgatacccg ccgattcgga
     9781 aaattcgaca atattgtaat tcaatgcctc agccgccgcc ggaaaagata ccgccagcag
     9841 ggaagccgcc aaaacagaac gcagcatatc cgtatcctta cttgaaaaac cgccaatcta
     9901 acatataggt acgctaaatt gcgttagcat aagacaacaa tttaaatatt cgatcggtct
     9961 gccttataat aaccgtcttt ccgatactga atcatattat gtccgaaccg ttgcacgtcc
    10021 tcgtcatccc cttatggtat ccacgatccg aacaggatgt ggacggaata tttttccaaa
    10081 atcaagcaca ggccctccaa cgcaaaggca tcaaaaccgc catcctcgcc cccatgttcc
    10141 gttatctgcg gaaagaaaca gcaagcatcc tgaccggtcc ttacggtttt gcccgatacc
    10201 ggcaaagcgg tttggacatc tacgcatggc gcagcatgta tttcttcccc gctttccgtt
    10261 tatcgacatc gaccgcatcc gctgggtgcg cgccggtttg aaagccttca aacactacat
    10321 ccgcgaaaac ggactgcccg acctgattca cgcccactgc atgaactatg caggcatact
    10381 tgcccaaaaa atttccgaaa aatacggcat tccctacgtt ttaaccgaac acagcagcac
    10441 catcacgcgc ggtctgatcc gtcaccacca atggcagccg atggaaaaag cagcggcgcc
    10501 cgcctccgca cgtctcgccg tcagccgcca tttcgcacac gtcctgcaac acaaatacgg
    10561 ctgcgaatgg cagtacctcc ccaacatacc gggtggaata ttcaagcaaa catttgaata
    10621 aaaagaaaaa aataacaaac cgcatttcgt gttctgcacc gtctcgcacc ttcgccgcct
    10681 caaaggacac gatgtcctgc tcactgcttt tgcccgggcg ttggcacaat gcccgcaact
    10741 gcgcctgaac atcggcggca gcggacagga agaacagcgg ctgaaacagc aggcggcaga
    10801 cttgggcatc gcccatgccg ttacattttt aggcgcactg caacccgaag caatcttgga
    10861 tttgatgagg aacagcgacg ccttcatcct cgccagccgc accgaaacct tcggcgtggt
    10921 ctatatcgaa gccctctcgc aagggttgcc cgtcatcgcc atacattgcg gcggcgcgga
    10981 atccatcgtt tcagacggca acggatacct cgtttccgtt gacgatgccg atgtcctagc
    11041 ctttctcaaa atatatgaac accatgccga tttttccgcc gaacagctca gaaccgactg
    11101 cctcgcaaca ttcggcgaag atgccgttat cggcagactg attgccgtat tccgtcaggc
    11161 ggcgcggaac acggcgcgaa aacgcccgaa aaatagatta aaatgacgca aaacaacctt
    11221 cggattcaaa tatgacccat accgtccacc tgcacttgga agaaaccgac aacccgacgc
    11281 tgcaacgcct gtgcggttct ttcgacagca acctcgattc gctcgccaaa gcactcgaca
    11341 tccatatcag ccgccgtttc gagcatttta ccttcaacgg tgcatttgca cacgccggca
    11401 aacgcgcact gctcaaactc ttggaaacgg cgcagacgcg cgacctaaac gacagcatca
    11461 tccgccttgc cgccgtcgaa acccaaaccg aagatgccgg gcatcaggaa aaaaatcacg
    11521 aacacgccta ttatttccga accaagcgcg gcagcatagg cggcagaacg ccacggcaga
    11581 acggctatat ccgcgccctg ctcgaccacg acatcgtatt cggcctcggg ccggcaggta
    11641 cgggcaaaac ctatcttgcc gtcgccgccg ccgtcgatgc gatggaaaaa caccaagtcg
    11701 aacgcatcat tttagtgcgg cctgccgtcg aagccggtga gaaactgggc ttcctgcccg
    11761 gagacctgac ccaaaaagtc gatccctacc tccgtccgct ttatgatgcc ctctatgacc
    11821 tgatgggctt tgaccgtgta accaagctga ttgaaaaagg cctgattgaa atcaccccgc
    11881 tcgcctatat gcgcggcagg acgctcaacg gcgcatacat catcctcgac gaagcgcaaa
    11941 acaccacgcc cgaacaaatg aaaatgttcc tgacccgcat cggcttcggc gcgaaagccg
    12001 tcattaccgg cgacaccagc caaatcgacc tgcccaaaaa catcaaatcg ggattgaaag
    12061 acgcacgtga gaaactgcac ggcgtggcag gactgtattt ccataccttc accggcgaag
    12121 acgttgtccg gcatcctctg gtgcaaaaaa tcgtcgaagc ctacgaatcg gcagaacacg
    12181 actgacactc caatgccgtc tgaaacccga tgcatggaaa atcgggcaga gaaaaacaga
    12241 gacggaagcc aagctccgta tccgtttctt tatccgtcca accgatacat caatcgaatt
    12301 ttctgcatcg gattgcctca tctccgcaaa acattgccga agtttcgcgg ttgcacgcct
    12361 gacgcacttt ctgccgcgcc acgccttcat tccgatcgct tgcctcaaac gtcttctgaa
    12421 aaggcgtaac gctgcataca tacatggtgg aatttgaacc gaaaaggtcg attttgacgg
    12481 tattgccgcc gctttccaat acggcgacac ggtgttccaa ctgcgccaga cgtgcttcca
    12541 aatcgctgat tctgtcggca aatgcaggca tggcggcaca cgcggtcaaa atgcccaaaa
    12601 tacatttttt catcaatttt tccttgttgc ttgaaccctg cccacccgga catccttttt
    12661 ccgggcggca gggtcagact ttatttgcag ggatgaagcc ccttacaaat cagaataata
    12721 catgaatcgg cactttatgc ttcatcctat gtgttgaaac ttcattgctc cttgtctgtt
    12781 gaccgttatt cccaatccgt tcccacaacc ttcggcttgg gtttgcgcct gcgacggcgg
    12841 cgttttttcg cctgtccttc gtttttttcg tgccgggcgg cggcctcgtt tttggtcatt
    12901 tccgtccgct gttccgccga tgccgtctga aacgccgtcc accactcggc aagtgcgcgg
    12961 tccgcattgc cggtttcggc gcgcaagagc aggaaatcat aggcggcacg gaaacgcgcc
    13021 tgtgcaaaca gtttgtgcgg acgtgcgcct ttgcggtttt caaactgcgg ctggaacatc
    13081 caaatttcac gcatcgtggc ggaaaagcgt tgcggcacgc cccaaccgcg ttcgacggtt
    13141 tcgcgcatcg tattgattgc atcggacagg gcgggcgtag gtttcaaacc ctgttgcaga
    13201 ttgcttttcc aatggcggtc caactctggc cacatcaggg ccgccagtac gaaaccgacc
    13261 gaaaccgatt tgtcggcacg cagccgctca tcggtatttt tcagggcaag cgccgtcatt
    13321 tttccggcga tgccgtctga aacgcgcaaa gcattgagca gcggatggat gtccggtatg
    13381 tcaaatccgt tcaaacgttt cagacactcg cgcgcgtgcc ctgaaaacag cagtttcatg
    13441 atttcgtcga acagccttgc cacaggttcg tgcttcagac ggcatatcga ttcggcaatc
    13501 ggtgcggcgg tttcttccga cagctcaaag cccaatttgc ccgacaggcg gatggcgcgc
    13561 aaaatcctga cagggtcttc ctgatagcgt tcggcggcat taccaatcat aaccagcctg
    13621 cgggcggcaa catcggcaat cccgttgtgg aaatccaaaa tctcttcttt ttcaggatcg
    13681 taatacaagg cattgcaggt aaaatcgcgc cgcatcgcat cttcttcgat gctgccgtag
    13741 gtgttgtcct tcatgattct gccgtgcgcg ttctgatgaa ccttcgcacc gccgcgaaac
    13801 gtcgttactt cgataatctc cgcaccgttc atcacatgga caatctgaaa acgcctgccg
    13861 atgatgcggc tgcggcggaa gagtttgtgc acctgttcgg gcgtggcatc agttgcgaca
    13921 tcgaaatctt tgggttcgat gccgagcagc aggtctctga ccgcaccgcc gaccacataa
    13981 gcctgaaacc ccgccccttt caggcggcgt atgacgtttt cggcggcaaa gctcaacatt
    14041 tcggcactga tgttgtgtct ttcggcagga atgaccgttt tactttccgc ttttttactg
    14101 ctccgaccgg aaggcagcat cttattcaac cattttttca gcataaatat tctgacccga
    14161 agagcggcat ctccacgggc aaccgtgttc agactgcatc aggcggtacg attccacaag
    14221 atgccgaaaa ccataagcaa aaggcgtaga aactacgccg tccgaatcac gccgccctcg
    14281 cggcggcaac gcgtcattat aacagattgc cccttaaaaa atcagaccct gcttttgtgg
    14341 cggagtctga aatttgatgc accttatata ttgcgtccct aagaagggat gattaacaaa
    14401 aattaacgcc ctttactttc ttttccgatt gggaacattc ccgtcgtaca aacaagcaaa
    14461 tgaaggaaca gaaatcacat tcaaacggcg ttccgccgcc tctacgccgc ttcccagtag
    14521 tcgatataaa gctgcaattc gaggttgtcg cgccattcgt tggcaacggg gcggtacacc
    14581 gtgcggatgt attcgggaat gtcttcgctg caacgccaga acatcgcttc aaattcgcag
    14641 ccgtcttttt gcaaccacgc ttttttgtgt ttgccttccg cgcccaaagg ctgctggcga
    14701 acgacgtgga actcgtcggt aaagctcggc ggcgcaaagc cctgccccca aacgtggcag
    14761 gcgaggtttt gcgcctgttc caacgtgatg tcgcaggcgg gcaggctgcc gtcagtaata
    14821 taggtttgcg acaaatcgtc ttcgcacacc atctcacgca cggcttcttc aaaggccgtc
    14881 tgaaacgcgg gaatattgtg ttcaagtatg cttaaacccg ccgccatcgc gtgtccgccg
    14941 aatttcaaaa tcaaatcggg atggcgtttg gacaccaagt ccaaagcatc gcgcaagtgc
    15001 agattgggaa tggagcgtcc cgaaccgcgc acttcgccgt tgtccgccgg cgcaaacacg
    15061 atggtcggac ggtaaaaacg gtctttgagg cggctggcaa caatgccgac cacgccttga
    15121 tggaagtcgt cgcgatacgc caccaaagtc gtctgacctg aaggcagggt ttcgggaaag
    15181 gcgttcaacg cgtctcgcag catagactgc tcgatttcgc ggcgttcgat attgaggttg
    15241 tttaaccgag ccgccagttc ctgcgcttcg gaatcatctc gcgccaacag gcaggcgatg
    15301 ccgaccgaca tatcgtccag ccgtccggcg gcgttgatgc gcgggcccaa cgcaaagccc
    15361 atatcaaacg gctgagcctt gcgccaatcg cgccgcgcca cttcaaacaa ggcgcggatg
    15421 ccggggcgca ttttgcccga acgcatccgt ttcaaacctt gcgacacgag gatgcggttg
    15481 ttgtggtcga gggggacgac atcggcgacg gtaccgagtg cgaccaaatc caaaaggccg
    15541 cccagattcg gctctttgat gccgtctgaa aaataattgc ggcggcgcag ttcggcacgc
    15601 aacgccgtca acacataaaa aatcacgccc acgcccgcca agcttttgct tggaaaaccg
    15661 cagccttttt ggttcggatt gacgatgatg cagtcgggta cggtatcggc tggcaaatgg
    15721 tggtcggtaa cgatgacatc caaacccaga gcctgcgcgc gcgccacgcc tgcaatactg
    15781 gcaatgccgt tatccaccgt aatcagcaaa tccacgcctt gcgcggcggc gatttcggcg
    15841 agttcgggcg ttaagccgta gccgtgctca aagcggttgg gcacgaggaa atccactttc
    15901 gcgcccatcg ccgccaaacc gtccaaaccg acggcacacg cggtcgcgcc gtcggcatca
    15961 tagtcggcga caatcaggat tttttccttg cgttcaaccg catccgccaa acggccggcg
    16021 gcggcttcgc aattcgtcag cgattgataa ggcaggaggg aagcgagttt gtcgtccaac
    16081 tcggcaggac tttgcacacc gcgcgaggca caaagccggg cgattaaagg atcggcgccg
    16141 gcggcgagca aatgattgaa aacgtcggta ttgacggggc gggtttggat tttgactgac
    16201 atagcgcggg gttttacagt gcgggtgtgc ttgaaatgcc gtctgaaggc ttttcagacg
    16261 gcattgccgt tcatttgtct gaggaattcg cggctgcaat cagagccata ggcaagacca
    16321 gcaccgcggc cgccgcatcc aatatcaaca aggggggcgt atataagata tttccaaaca
    16381 gcttggattt gtcggtatgt ttttcagtaa ccgtataata aatatcggcg ggcacacttt
    16441 gctcaaaatg ataatcggcg ttcagttttt gcggcgtggc gtagtatttg cctttggcgg
    16501 atacgcagcg cgtgtaaatg gtccgattgt cgagtttgac cgctttaaac tcaagctgtt
    16561 tcagcttggc gatgtcgtca ggtctgccgg tatcatagcg caggcaaaga cctccggtac
    16621 tgaaattctg gctgccgggc gcttcgaatt tgaccggcag ggcttggtgg cgggcatagc
    16681 tcggggtatc ctcaactatt tggaagggct tgtccaaccc ggccttcaaa aggcccgtca
    16741 gcttcgccga atcttcggga ttgacggcga accagtattt cccgcccatc atcaccaggc
    16801 tgcccttttc caattgggca ttgtcttcgg caaccacacc gaaggcgcgg atttggtctt
    16861 tgtcaacgtg tttgcgggta attgtttggc tgaccgggtt gttcatcccc cgcaacatca
    16921 tcgtacagcc gttcagtatc aaggttgccg ccaaaacggc ggcggtcgtt ttacggaaca
    16981 acattttatc ttccaatcta aataacggcg gatgccgtct gaaaatatgc ctaaatttcc
    17041 gcgcccccgt tttccatcac ggttttcaat tcttgaaaca gggcgcggaa gtttttcggc
    17101 ggtttgtcct gttcctgttc ttttttggtg ttgcggacga gcgtcctcag cctgcccgcg
    17161 tcggcgtttg gaaaatcgga cataaactgc gtcagcgcgc cctcgtctgc caacaaccgt
    17221 acacgcgcct gttccacgcg ttgcaaaaag gcgttgtgcg ccgcatcgtc gccgcgcagc
    17281 ttggcaagga acgcctcgat gggcgcggga tcggtatcgc gcatcagacg tccgataaat
    17341 tgcgcctgac gtttgagcgc gccgttggac gtaatttttt tataggtaac gacggcctcg
    17401 tacaaatctt catccaaacc gatttttttc agcgtgtcgt ttgagagcct ggtcagttcc
    17461 atacccaaat cctgcaaacc gttcatctgc tttttcattt gggttttgct gatccactcg
    17521 tcttcttgtt caaacatctt gattctcgaa ttttttccaa tagcgtattt taacagaatg
    17581 ccgccgcgtt cagacggctt tttgcaccgc acgccctgca agcggcacgc ttttgcccct
    17641 gccgcccgaa acggttaaaa tgccgcaacg ctccaccaag aaagattgtt atgctgttca
    17701 accacaccgc ttccgaactc ctcgacctct gccgccgcac gctcgacttg gcaaaagcag
    17761 cgggcgcgac cgccgccgaa gccgacttca gcgaatcgct cggacaaagc gtcagcgtgc
    17821 gcttgggcga aatcgaacaa atcgagttcc agcaggacaa atcgctggac attaccgttt
    17881 acgtcggcaa acgcaaaggc cgcgccggca ccgccgactt ctccgaacaa gccctgcgcg
    17941 acaccgtccg cgccgccgcc gacatcgccc gccacaccgc cgaagacggt tgcgccggac
    18001 ttgccgaccc cgagttgatg gcgcaacaca tcggcgaccc cgacctttac cacgaatggg
    18061 atttggatac ggaagccgcc gtcggcttgg caaaacaatg cgaacaggca gcgttggaca
    18121 cggacagccg catcgaaaac tccgaaggcg cggcggtgca aaccggacac taccaatacg
    18181 tttacggcaa cacccacggt tttgccgcac accggcaaag cacgcaccac agcatttcct
    18241 gcagcgtcgt tgccgccgac gaaaacggaa tgcagcgcga ctactggtac gattccgcct
    18301 gccgccatcc ggatatggac agccccgaaa ccatcggtca aaccgccgcc cgccgcactt
    18361 tgcgccgact cggcagccgc agcatcccga caggcagcta ccccgtcctc ttcgatacca
    18421 ccgtttcggg cgggctgatc ggacacctcg tcggcacact ctccggcggc gcgctctacc
    18481 gccaaagcag tttcctgatc gacagcatcg gcaaaaaagt cctgcccgat ttcctcaacc
    18541 tgcgcgaaga accgcacatc ccccgctctt tccgcagcag ctattttgat gcggaaggtg
    18601 ttgccaccgc gccgcgcttc gtgattcaaa acggcattgt cgaaggctat ttcctcggca
    18661 gttacagcgc gcgcaaactc ggaatgcaga ccacgggcaa cgccggcggc gcgcacaacc
    18721 tgtatttgaa ccatacgcac gaaacgcaat ccgacctgct gaaagaaatg ggcacgggat
    18781 tgctggtaac cgaattgatg ggacagggtg cgaacaccat caccggcgac tattcgcgcg
    18841 gcgcggcggg tttttgggtg gaaaacggcg tgattgccta ccccgtccac gagattaccg
    18901 ttgccggacg cttgcaggat atgtaccgcg acatcgtcgg cgtggcggat gacgctttgc
    18961 ggcgttcgtc caacaaaatc ggttcgattc tgattgcggg gatgacggtt gccggaagct
    19021 gaacgccctg ccgatgccgt ctgaagacga aggcggcgcg ttttgccccg tgcctgcccg
    19081 tttgccgttt tatgccgcac cgccttttac cggcgacatt gcacattttt ttcatcagca
    19141 ctgattttcc cttatccgtc aaaatactaa gccacttgcc cacaacaggg tacacacaag
    19201 ttcaggataa ggcgggcggg gattggggac ttttccgcca tcggcaaaac ggcagacaaa
    19261 aacggcggga cattgcctgt cccgccgttt tcaaatatac atttgaccgt acatcaggaa
    19321 ttaagctgcc tgaatgttgg cggcctgttt gcctttaggg ccggtggtta cgtcgaaaga
    19381 gacgcgttgg ccttctttca gggttttgaa accttccata ttgatcgctg agaagtgtgc
    19441 gaacaaatct tcgccgcctt cgtcaggagt gatgaaacca aaacctttag cgtcgttaaa
    19501 ccattttacg ataccggttg ccattagaaa cttcctatac ttaaaaatta acaaaatcag
    19561 caaaataagg catacagcaa acttaaacgt tcagcgtttc tacccagctt tatatacttg
    19621 gtcgagtgcc gacttttgct taaccgtctt tttacatttg ttaagacaaa acgtcaagct
    19681 atgttttcaa aaaagtggga aaataggcga aagtaccgaa aatacaacaa aaatgccgtc
    19741 tgaacgcggc tttcaaatgg gacacgctat gaccgcgcaa caccaatccg acacgctttt
    19801 gcaccgcctg aacaccctgc cgccgaaacg ttacggcgta tttttattga acgacgatta
    19861 caccacgatg gaatttgtcg ttgaaatcct gaccgaaatc tttatgctcg ggcaggaaca
    19921 ggcggtagcg gtaatgctct cggttcatca cgaaggcaaa ggcctgtgcg gcacttacac
    19981 gcgcgatatc gcccaaacca aacagcaaca agtcatgcag cgggcaaaag ccgaaggaca
    20041 tccgctgcaa tgtattgtcg aggagattta atatgcttgc ccctgaattg gaacagattt
    20101 tgcagcagct ttaccgcgag gcgcgtaagg ctcattatga atttatcagc ctcgagcatc
    20161 tgcttttggt actcatcgaa gaagatgccg ccgtgccgaa cgtcttaaaa ctctgcggtg
    20221 cggatttaaa agcggtgtcc gaacagctcg ccgccagcgt tgccgaaaac acccccctga
    20281 ttcccgacca ccttttagac acggtcgaaa cccggcccac gctcggcttc caacgcgtca
    20341 tccagcgggc gatggtgcat acccaatcgg caggcaaagg cttggccgag cctttggacg
    20401 ttttggtcgc gctgatgagc gaaaccgaca gccacgccgt ctatttcctc aagctgcaat
    20461 cggttacgcg ttttgaagtt ttgcgctgta ttgcccacgg ctctcccgat gaagatgaag
    20521 acgatggcaa ctatttttca gacggcatgg acgacgataa tgagaaccgc accaaaccgg
    20581 gcaaaaaccc tttatcggcg tacaccgtca acctcaacgc cgaagtcaaa gccggccgta
    20641 tcgatccttt gattggtcgc aaacacgaaa tggaacggct ggtgcaaatc ctgtgccgcc
    20701 gccgcaaaaa caatccgctt ttggtcggcg aagcaggcgt gggcaaaacc gcgctggcgg
    20761 acggtttggc acatcaaatc gtcaacgacg acattcccga cgcgctcaaa gaggccgaag
    20821 tgtacgcgct ggatatgggt tcgcttttgg cgggcacaaa ataccgcggc gactttgaag
    20881 cgcgggtcaa atccgtcttg aaacagctcg aaaaaatccc gcacgccatt ttattcatcg
    20941 acgaaatcca caccatcatc ggcgcgggca gcaccggcgg cggcacaatg gacgcgtcca
    21001 acctgctcaa acccgcattg gcgaaaggtt cattgcgctg tatcggcgca accacttacg
    21061 acgaataccg tactattttt gacaaagacc acgccctaag ccgccgcttc cagaaaatcg
    21121 acgtggtcga acccaccgtt gccgaaaccg ttcaaatcct gcgcggcttg aaaccgatgt
    21181 tcgaagcctt ccaccaagtc cgctacacgc aaggcgcact cgaagccgcc gccgaactct
    21241 ccgcccgcta catcaacggg cgcttcctgc ccgacaaagc catcgacgta atggacgaag
    21301 caggcgcggc gcaacggatt ctgcccaaat ccaaacagaa aaaagtcatc ggcaaagcgc
    21361 aaatcgaaac cgtcatcgcc aaagtcgcgc ggattcccga aaagaccgtg tcgcacgacg
    21421 acaaacaagt gttgcaattc ctcggccgcg atttgaacaa tatggtttac gggcaggaag
    21481 acgccatcga cgcgctggtt tccgccgtca aaatgtcgcg ttcgggcttg gggctgcccg
    21541 acaaaccgat aggcagcttc ctcttctccg gccctaccgg cgtcggcaaa accgaagtcg
    21601 ccaaacagct tgcctactca atgggcgtac cgctgcaacg ctttgatatg tccgaataca
    21661 tggaacgcca cgccgtatcg cgactcatcg gcgcgccacc gggctacgtc ggctttgaac
    21721 aaggcggcct tttgaccgaa gccgtcaaca agcagcctca ttgcgtgttg ctcttggacg
    21781 aaatcgaaaa agcccatcca gatattttca acgtcctcct gcaagtcatg gacgcaggca
    21841 aactgaccga taacaacggt aagagcgccg atttccgcaa cgtcatcctg atcatgacca
    21901 ccaacgcagg cgcagaaagc ctcagccgac ccagcctagg ctttaccgcc aaacgcgagc
    21961 gcggcgacga aatgcaggca atcaacaaac tcttcacgcc cgagttccgc aaccgcttgg
    22021 acgcgatcat cccgtttgca cctttgtccg aacccatcat cgtcaaagtc gtggacaaat
    22081 tcctgctcca gctcgaacac cggctcctcg acaaaaaagt cgaagccgaa ttcacaccgg
    22141 cattgcgtaa atacttggca gaaaaaggtt tcgacccgca aatgggcgcg cgcccgatgc
    22201 accgcctgat tcaggaaaaa atccgcaaac cgctcgccga cgaactcctg ttcggcaaac
    22261 tcgccgacgg cggctttgtg cggatagact gggatgcggc gaaagaagaa gccgtattga
    22321 agtttaagaa aagcaaggtc aaaataaaaa ccgcctccgc ataaaatcaa aaatgccgtc
    22381 tgaaaggttc agacggcatt ttgtcaaata tcggccccta ttccggcaaa cttctccaca
    22441 aacgccgcga ttttttggaa gacgctttgt ttcttcgtca gatattgcgg gttgagcgga
    22501 ctcattttcg gcagggtttc ggtcagttcc gtgccgtttt cgctggcata gccgcgtttg
    22561 agcgagccgg tcaaatagcg tttggcggcg gtttcgttca gaccttcggc ggcaatcaat
    22621 cctgccgctt cgtgccgcat cacctcttgc gcgtaggtgt aaaaggtttc cagtatggcg
    22681 ggaacgtcgg gcactttatc caaatccgta tcgttgatga aatccacaat caggccctct
    22741 ttggcgcggt ggccgatgct ggcgcggatg atgcggcgga tttcttccac cagctccgct
    22801 ttgcctttga tttttttgtg atgctcgaaa accagttgca ggatgtaatc cagattgatt
    22861 tcctgcgatt tgagcaaatc cacctcaaaa accacatcgt cccagtcgat ttttgactgt
    22921 tctttctgtt cgcctgcttt ttggcggcgc agccagtctc ggatgtcgtt ataggcggaa
    22981 cggtagtcct gcaccgccct ttcagacggc atcggcactt tccgcatttc ctgcacgtct
    23041 tcgtcgctca ggtagtattt ttcctgaaac gccttcatcg catcttcgtc cgccgcgtcc
    23101 acattctgca attcgcgcag cgcggcaaat tcatcgtagt tctgcaatac gttttccgcc
    23161 cgcaggtatt cgccgaagag tttggcaaaa tcttttttgt ctttttccgt ttcgattttg
    23221 tcgggatcgg ggaaacgctc gtgcaattct tttgctacat ccagataacc gcgccgtgct
    23281 tcgccggtct ggctgtcggt atagccgttc atgtattctt cgtagctttt ttccagcact
    23341 atatttttgg tgtttttgtc gccgaagagc gtgatcgcat caatggttgc ctgctccaag
    23401 tcgcggaagc agacaatgtt gccgaaggtt ttggtagcat cgtaaatgcg gttggtgcga
    23461 gaaaacgcct gcatcaggcc gtgatagcgc aggtttttat cgacgaacag cgtattcagc
    23521 gtcggtgcgt caaaacccgt caaaaacatg ccgaccacaa tcagcaagtc cacttcccgg
    23581 tttttcaccc gttttgccaa atcgcggtag tagttctgaa aggctttgct gtccgtgccg
    23641 aaattggttt tgaaacaggc gttgtaatcg ttgatggcag cctgcaaaaa ttctttcgcg
    23701 ctgctgtcca tcgcttccgg ttcaaaggtc tcgtcaacaa tttcaccgac ggcgttttgc
    23761 tcttcgttgg cggcaaagga aaaaatggtg gctgttttca acgggtgcag gctgcctgcc
    23821 tgttgtgttt tgaacgcttc gtaatagcac ttcgccgcat ccacgctgct gacggcaaac
    23881 atggcgttga agcctttgcc gcccgcattc aagcggtgcg ttttctgcct gaaccggctc
    23941 aggatatatt gcgtgatttc gcggatgcgt tcggggtgca gcagggcttg gcggttttcg
    24001 gcggcactca gttccttctc gtcctgttcc gcttccacgg ctttgaactg cgggcgcaca
    24061 tcgttgtaat ccactttgaa tttcaatact ttttcatcgc ggatggcatc ggtaatcaca
    24121 taagaatgca gctcccgccc gaacacgccc gccgtggttt ccgcgcccaa agcgttttcg
    24181 ggaaaaatcg gcgtgccggt aaagccgaac tggcagaatt ttttaaattt ctttttcagg
    24241 tttttttgcg cttcgccgaa ttgcgagcgg tggcattcgt cgaaaataaa tacaacctgc
    24301 cggtgataaa ccggcagatt atcttcactt ttcatcaggt tgttcagctt ttggatggtg
    24361 gtaacgatga ttttgttgcc gtctttttcc aaattgcgtt tcaagcccgc cgtactttcc
    24421 gagccgttta cgctgtcggg cgaaaaacgt tggtattcct tcatcgtctg atagtccaaa
    24481 tccttcctgt ccaccacgaa gaaaaccttg tcgataaacg ccgattccgt cgccagacgc
    24541 gccgccttaa agccggtcag cgttttgccg ctgcccgtgg tatgccaaac atagccgccg
    24601 ctttccggcc cgctccaatt cttcgcctgc gccgagctgt tgattttcca caaaatgcgt
    24661 tcggcggcgg caatctggta cggccgcata atcagcagcg tatcattcgc atcgaacacg
    24721 ctgtaatgca gcaaaacgct cagcaatacg cttttctgca ggaacgtggc ggtaaagtct
    24781 ttcaaatcct taatcggatg attgtccgac cgcgcccaat tcatcgtgaa atcgaagctg
    24841 tttttgtcgc gcttggtggt gttggcgaaa tagcgcgtgt ccgtgccgtt ggaaatcacg
    24901 aagatttgca ggaatttgaa cagcgaattt tcgctgttga agctctcttt gctgtaacgg
    24961 tgcacctgat tgaatgcctc gcgcaccgcc acaccgcgct ttttcaattc aatctgcacc
    25021 agcggcaggc cgtttaccaa cacggtaacg tcataacggt ttgcatgcgt gcccgtctgc
    25081 tcaaactggt tgataagctg cacatggttg cgggcaaggt ttttcttgtc cagcagataa
    25141 atgtttttca gacggccgtc atcaaaagcg aaatcgtaaa tatggtcgtc gtggattttg
    25201 cgggttttat cggtaatgtt ttcagccggc ctgtccagat attccgtcaa aaaccgcgcc
    25261 cattcgccgt ctgaaaacgc cacatcgttc agccgctgca actgggcgcg caggttttcc
    25321 agcagcctgc tttggctgtt caaatccttg cggtattcgt acccctgatt ctgcaaatcg
    25381 gcgattaact ccgcttccaa ccggttttcc gattggtagc tgcccgactg ttcgattttt
    25441 tcatattggt cgagcacgat gaaattcggc gtttcggcga tgggtttggt ttcgaggttc
    25501 atgggtcgtc ctttgcggtg tcgggtttta tagtgaatta aatttaaacc ggtactgcgt
    25561 tggctcgcct tagctcaaag agaacgattc cctaaggcgc ccaagcacca ggcgaatcgg
    25621 ttccgtacta tttgtactgt ctgcggctcg ccgccttgtc ctgatttttg ttaattcact
    25681 ataaacggtt tgatgttcgg attgggctgc ctgaaaaagt gcaggctgct ttttcaggca
    25741 gccttgggga aggcaagcag ctgttcgcgg taatattcgt attgtttgcg gcgcagggcg
    25801 atttcgtgcg gcaggccttc gctgacggag tgggtcaggg tatcgaattt gcctaggatg
    25861 gcgacgattt tttcctgttc ggggagcggg gggatgggga ttttatattg cataatcgct
    25921 gctttgctgc cacgcggcat ttttgcacct ttggcgtgct tcatattaaa agcaaaaaat
    25981 ttatcatcgg ccaatacctg atacagatat tttggattaa ccttttcatc tgttacccgg
    26041 ataaccaaaa catcaccgtt tgttccgccc gtgcagtctg cctgccagat ttttttcaaa
    26101 tacggacgga tatttccaat caaaatgtca ttgacaatat attctgtcat ttttccttcg
    26161 cttggaacat aaccggataa ctttttacct tctctgtttt gtaagagatt atccacgcca
    26221 acgtaattat gttcgttcag tttatccgaa caaatacggt tttttgaata ttcggcaacc
    26281 tcccccaacg tcttccaaac cacatttttc agacggcatt gatagccgtc agctatcccc
    26341 cccccgattt gattgtcaaa atctaaaagt aagtcgcggt aataccggta ttggcgtttg
    26401 cgcagggcta attccgcttc cagcgtagct tccagcgtag cttccagctc ggtgaatttg
    26461 tcaagtattt ttacaatttt ttgttgggtt tccaatgatg ggatggggat tttatagtta
    26521 tctgtatctg gtgttgctat ttgtggcatt tgcatttttg atccgatatt acgaaaatag
    26581 ttttcttgtg tttttaagaa ataataaaca tattttatat aaatatgttt attaactgaa
    26641 tgataagacc acatttcatt tttatgcgaa aatggtttat cgtaatattc gaattcaata
    26701 atacctcttg atttaacgat aatagaaggc tctctatgga tatccttatc aggaacatca
    26761 tcaaagtcaa ctaatgcaaa agtttttcct cctgcaaaaa tctttaacgg cgcattatct
    26821 ttatgcattt ctttcatttg tccagcagtt atcttagttc cttttgtacg aactaaaacc
    26881 tcccccaatg gcttccactc caccggtgcc gtctgaatca tctcaatcaa ttttttcgct
    26941 ttgctttgca tatccatggt tgttttccgt ttccttcccg ctgcggcggg atgtggggcg
    27001 gttgcggggc gggtttcggc tcactctgtc cgtttgccgt tgtttctgcg ggctgaaacc
    27061 cgtcctgctt tcaggctgcc tgaaatcgtg tgcaggctgc tttttcaggt agcctgaatg
    27121 cggttatttt ttgccgtttt gatgctgttc ggttgcggca agccttttct gttcttcgtt
    27181 ctcaagctgt ttgatgcttt tctcgggtac gggctggttt tcaggcatga tgccgcccag
    27241 ttcttcaatg gtttggcgca ctttttgtcc gacttcgaaa tgcacccggt ttgcctgtgt
    27301 tttgccttgg atgtttttgc ggcgtagttt ttcttcggtt tgcgtggcgc gaaacagatt
    27361 ggcagccggt tcgctggcgt tcatgtggtc taggattctc tggcttttct tcaagccttt
    27421 gcggctgtgt atgccctgct tgtccagtcc tccgtatagc ccccgatagc cgtggttttg
    27481 gaagacggcg tactcaacgg gtttttctac gcctgcatcc ttggctgcgg cggcaaggtc
    27541 ggtattgtgt tcgcgcagct gcctgcgaag cagcaggcgt tgtttgtctt cgcccaaact
    27601 cctgaatgct gcctcgtctt gcagctcttg gcggcgggct tgcacggcaa agtaggtttg
    27661 ccctgcggca atgacgcttt tggacggatc gccgttttgc acaatcaggt agcaggcata
    27721 gcgggaaagg cggtaatccg ccacttcgcg ctgcccgcct ttcccaaggg ctatcatttt
    27781 gttggtttca acaaaatgat gatttttgtc atttcctgat gtttcgcaag cggttatggc
    27841 tttatcaatg gcccgttgga aattgcgcca ttcggaatat tccaagattt gctgcaaggt
    27901 gcgcgccgac caatattcat tgcctgtttc gtcgatgtgt ttggcgtttt caaaagcgtt
    27961 attttctgtt gtcatgtatt ttccccgaaa atttcttgat ttgaacttgt aaggattgct
    28021 tacaggttgc cgcccgtatt ttcagacggc ctctttccca ttcagttgtt gcaaaatatg
    28081 caacaactgc ctaggtttca atctctgcaa tcacttcgtc aatttcacgc cgcagccgtt
    28141 cgattttggc gacggtttcg ctgatttcgg cgttgagctg tctgatgtcg atgacctcgc
    28201 gggtgtcttc ggcttcgaca tagctgctga cggcgaggtt gtagccgttg tctttgacgg
    28261 tttgctgggc ggcgttttgg gcgatatgcg gcacatcggc tttgtcggcg aagagtttga
    28321 cgatttcggc aatgtgttct tcggttaaga cgttgttgtt ggtttctttt ttaaagaagc
    28381 cgcctgcatc gatgaattgg atgtcggtat tgtctttgtg tttggacaaa accaggatat
    28441 tgacggcgat gcaggtgccg taaaagagat tgggcgcaag ggcaatcacg gtttccacat
    28501 agttgccctc caccagatat tggcggattt tctgctctgc gccgccgcga tagaaaatgc
    28561 cggggaatga gacgatagcg gcgcggcctc tgccggaaag gtagttcagt gcgtgcagga
    28621 tgaaggcaaa atcggctttg gatttcggtg cgagtacgcc tgcgggggca aagcggtcgt
    28681 cgttgatcaa ggtggggtcg tcgctgccta tccagtcgat ggaatagggc ggattggaga
    28741 cgacggcatc aaagggtttg ctgtctttga gtttggggtt ggtcagcgtg tcgcccaatt
    28801 cgatgtggaa tttgttgtaa ttgacgttgt gcagaaacat attcatgcgg gcgaggttgt
    28861 aggtggtgtg gttgatttcc tgcccgaaga agccttcttc gatgatgtgt tcgtcaaact
    28921 gttttttcgc ctgcaagagc aggctgcccg agccgcaggc ggggtcgtag attttgttga
    28981 ctttctcctg cccgtgcacc gccagccgcg caatcagctt ggagacgctt tgcggggtga
    29041 aaaattcgcc gccggatttg cctgcgttgg cggcgtagtt ggaaatcagg tattcgtagg
    29101 catcaccgaa aaggtcgatg cggtggtctt caaaattgcc gaaatcgagt tccgccacgc
    29161 ctttaaggac ggcggcaagg cgtttgtttt tgtcggcaac ggtgctgccg agccggctgc
    29221 tggtggtgtc gaagtcgtca aacaagcctt tgatgccttg ttcggacggg tagccggagg
    29281 cggagctttc aatcgcggta aagatttctt tcagcttggt gttgagctct tcgttttgat
    29341 gggcttcggc ggcaatattg caaaaaagct ggccggggta gatgaaatag cctttgactt
    29401 tgacggcatc gtctttgatt tcgggcgtga tgatgctgtc cggcatagcg gcgtaatcaa
    29461 tgctgctgtc gccggcctgc atatagtcgg tgaagttttc gctgataaag cggtagaaaa
    29521 gtgtgccgag aacgtattgt ttaaagtccc agccatccac cgcgccgcgt acttcgtcgg
    29581 cgattttcca aatttggcgg tgcagttggg cgcgttgttg catttcggtc atcatcgaaa
    29641 tccatatata aagttaaaca aatcaaaatc gcctgatatt ttcagacgat ttttttacgg
    29701 gcattcaaat taagccaaat tttccagcat ccatttgacg tagcggttga cacctgcttt
    29761 gacatcgaaa aattcttcct tatatccggc ttcgcgcaat ttggtgatgt cggcttgggt
    29821 gaagccctgg tatttgcctt tgagcgcgtc gggaaagggg atatagcgga taagttcttc
    29881 ttctaccaac tctttcaagc tcaattcaga tttgccttcg gcggcgcggc aggcgttgac
    29941 ggtggcggcg gcgagttcgt tgaactgttg gctgcggccg gtgccgaggt tgtagatgcc
    30001 ggaaagttcg ggatggtcga agaagtagag gttgattttg gcgacgtctt cgacgctgac
    30061 gaagtcgcgg gtttgttcgc cgttgccgta gccgtcgttg ctgccgaaca ggttgacgta
    30121 accgtgttcg cggtattggt ggaaatggtg gaaggcgacg gatgccatgc ggcctttgtg
    30181 ttgttcgtgt tgtccgtaaa cgttgaagta gcggaagccg acgacttggg cggtgagacc
    30241 ttctttcatg cggcggcgca atacttggtc aaacaggaat ttggagtagc cgtacacatt
    30301 aagcggtttt tcgagttcgc gctcttcgcg gaagatttcg cctttgccgt aaaccgccgc
    30361 actggaggca taaaggaagg ggatgcgttc gtcctgacac cagtccaaca aatccagcgt
    30421 gtactggtag ttgttttcca tcatatacaa accgtcgtgg ttcatcgtat cggaacacgc
    30481 gccttgatgg aaaacggctt cgatgttttg ataaggtaaa atgtgttccc tcacttggcg
    30541 gataaattcg tgtttgtcga ggtagtgggc gatttcgcac tcggcaaggt ttttgaattt
    30601 ttcgcctttt gtcagattat cgacggcgac gatgtcggta atgccgcgtt ggttgagggc
    30661 tttgacgatg ttgctgccga taaagccggc cgcgcctgtt acgatgatgg tcatattcgg
    30721 tttcctttgt tttgagggtt tcagacggca ttggatggtc atgccgtctg aaaagattta
    30781 ttgcccgtcc agtgcttcaa ccaactctgc aaacgagcaa accgccgtac cgagtttcgc
    30841 cacgacaacc ccggccgcag tattggcaag gtacatggct tcgggcatgg tgcagcctgc
    30901 cgccagcccc aagcccattc cggcaatgac ggtgtcgcct gcgccggaca cgtcgtaaac
    30961 ttcttgggcg cgggtgggct ggtaaatggg ttcgccttcg ctgaacaggg tcatgccctc
    31021 ttcgctccgg gtcagcaaaa cggcggtcaa gtcgaggtgg cggcgcaggt tttgcgcttt
    31081 ttcggtcaga tcgccttcgt ttttccaact gccaaccact tctttcaatt cggcgcagtt
    31141 gggcgtaatc agagttgcgc cgacatattt ttcgtaatcg tcgcctttgg ggtcgattaa
    31201 gacggttttg ccgacatgtt tcgcccaatc gatcatatcg gagatatgcg acagaccgcc
    31261 tttgccgtag tccgaaaaaa tgattgcgtc gtattcgggc aagatttcgc ggtatttccg
    31321 cttgatttgt tccaacactt cgcggttggg atgttcttca aaatcaaggc ggataagctg
    31381 ctggttgcgg gcgacgacgc gcagtttgac ggtggtggcg atttgtttgt cgcgcatcag
    31441 ataggaggcg acgccgtcct gcaccatcag cgcgtcgagc gcggcggcgg cttcgtcgtc
    31501 gccggttacg gacaacagcc ctgccctgcc gcccaacgaa gcgatgttgc gcgcaacgtt
    31561 cgccgcgccg cccgcccgtt ggtcgattcg tccgattttt gccaccggca cgggtgcttc
    31621 gggcgaaatt cgggacacgt cgccaaacca atagcggtcg agcatcacgt cgccgacaac
    31681 aagaactttg gcttgtgcga aacgggattt gagggtttct tgttggaact tggcgggcat
    31741 attgcctccg ttgtgttttg ttcagacaac cttaacctgt aagaccgcct gaaaatcagt
    31801 ttgcttcaag gtttgccacg cggttcactt cgcgcaatac ggcgaccgga tcggcggctc
    31861 gggtaacggg gcggcccatc accagataag tcgaacccgc cgccaaggct tcggcgggtg
    31921 tcataatgcg gcgttggtcg tcgttgttgc cggcaacgtc caagcggata cccggtgtaa
    31981 ccaagacaaa atcccgtccc aattcgcggc gcagcggcgc ggcttcttgg gcggaacaga
    32041 ccacgccgtc caagcccgaa ctttgtgcca gttttgccaa acggatgact tgttcttcag
    32101 gggcggtgtt caaaccgatt tccgccaaat cgctttgctc catgctggtc aacacggtta
    32161 cgccgattaa gagcggtttc gtgccgtatc cggcaacggc ttcggcggcg gcttccatca
    32221 tacgtctgcc gcccgacgcg tgcatatcga ccatccacac gcccatatcg gcggcgactt
    32281 tacacgcctg cgcgacggtg tggggaatat cgtggtattt caaatcaaga aagagtttga
    32341 aaccctgatg aatcaggctt tccgccaaac tgcgccccgt agcggtaaac agctctttgc
    32401 cgattttgat ttggcacaac gccggatcaa ggttgcggac gaatccgagc gtgtcttttt
    32461 cgttggcaaa atcaagggca acgatgacgg gggtacgttg ttgcggagtt tggaagtcgg
    32521 tgattaaagg attcatggtg ttttcgtttc gggttttcag acgaccttta tcgtcttttt
    32581 cgcagtatag cggattaaca aaaaccggta cgtcgttgcc ccgccccggc tcaaagggaa
    32641 cgattcccta aggtgtccaa gcaccaggcg aaccggttcc gtactatccg tactgtctgc
    32701 ggcccgccgc cttgtcctga tttttgttaa tccgctataa caacgtaaat tcaaaccgta
    32761 ggccgggcat tgatgtccga cctacgcgga ctcaagcccc cgacaaactg tttcggataa
    32821 atttgaaccg gtacatcagc cgtcattccc gcgcgggcgg gaatccattg ataaaactca
    32881 gtttatcaga tttaaacagc gattgcccaa attcagaaat ggattcccgc ctgcgcggga
    32941 atgacgccga atgattattt ttcggttgca acacgctgtt tatcaaaaac aggtcgtctg
    33001 aaaacctgat tttctgtttt cagagtgcga agcctgcccg tgggtacgac cttttcgatt
    33061 tatttgccca atgcgcccaa tacttcggct ttcacagctt ctactgcttg ggtaccgtcg
    33121 actttgatgt atttcggcgc gtgttcgcct tccagcttgc tgtaaaaatc aaccaaaact
    33181 tcggtttgct cgtggtaaac ggcaagacgt tttttcacgg tttcttcttt gtcgtcgtcg
    33241 cgctgaatca aatcttcgcc ggttacgtcg tctttgcctt cggttttagg cggattgtag
    33301 gtaacgtggt aagtacggcc ggaagccaaa tgcacgcggc ggccgctcat gcggtcgaca
    33361 atcacgctgt cggatacgtc gatttcaacg accgcgtcca aacccacgcc tgcttccacc
    33421 atcgcttcgg cttgcgccaa tgtacgcggg aaaccgtcga acaggaaacc gtttttgcag
    33481 tcgtcttgcg cgatgcgttc tttgaccatg ccgataatga tgtcgtcgcg caccaaaccg
    33541 ccttcatcga tgattttttt cgcttccaaa cccaagggcg tgcctgcctt aatcgcggca
    33601 cggagcatgt cgccggtaga gatttgcgga atgccgaacg cggcggtgat gaattgcgcc
    33661 tgagtgcctt tgcccgcgcc cggtgcgcct aaaagtaatg ctttcatgaa gtgatccttt
    33721 taaatgtgtt tattggaatc aaacagatgg tttacgattc tacacggctt ggcgcaatag
    33781 ttcaaaaacc aactgcacaa actgccattt gcaagcaaat gttttgcata cctttgccga
    33841 gcctgatatt cacaaggcgg caactttttg ggacaatccc caaagtttca aaacatcaca
    33901 ccctaccaaa aaggaacatc cgtgaaacgc atttttctgt ttttggctac caatatcgct
    33961 gttttggtcg taatcaacat tgttttggcg gttctgggca tcaacagccg gggcggcgcg
    34021 ggcagcctgt tggcgtattc cgccgtcgtc ggcttcactg gttcgattat ttcgctgctg
    34081 atgtccaaat ttatcgccaa acaatcggtc ggtgcggaag tcatcgacac gccgcgcacc
    34141 gaagaagaag cctggcttct gaacactgtc gaagcccaag cgcggcaatg gaacctgaaa
    34201 acgccagaag tcgccatcta ccactccccc gaacccaatg cctttgccac gggcgcatcg
    34261 agaaacagct ccctgatcgc cgtcagcacc ggtttgctcg accatatgac gcgcgacgaa
    34321 gtggaagccg tgttggcgca cgaaatggcg cacgtcggca acggcgacat ggttacgctg
    34381 acgctgattc aaggcgtggt caataccttt gtcgtgttcc tgtcgcgcat tattgccaac
    34441 ctgattgccc gaaacaacga cggcagccag tcccagggaa cttatttcct agtcagcatg
    34501 gtattccaaa tcctgttcgg cttccttgcc agcctgattg tcatgtggtt cagccgccaa
    34561 cgcgaatacc gcgccgacgc gggcgcggca aaactggtcg gcgcaccgaa aatgatttcc
    34621 gccctgcaaa ggcttaaagg caacccggtc gatttgcccg aagaaatgaa cgcaatgggc
    34681 atcgccggag atacgcgcga ctccctgctc agcacccacc cttcgctgga caaccgaatc
    34741 gcccgcctca aatcgcttta aaccgatttg aaacgacaaa aaaccgtacg cacaagcagt
    34801 acggtttttt cgtatatgcc gactgaaaac atcaggtttc cataccggca ccaaccgtat
    34861 ccatttccgc accggatacg ccgcattgat atatccccga caaaccgtgt ccggacagaa
    34921 aatatcagat aaaacagcgg taaaaatgcc gtctgaaacc aattagggct tcagacggca
    34981 tttttactgc aaaaccttat gcgaacggat gatgcagcac aatcgtttct tcgcggtcgg
    35041 ggccggtgga gacgatggcg accggcgcgc cgcagacttc ttcaatccgt ttcaaatatg
    35101 ctttggcatt ggcaggcaat gcgtcgtagc ttttcacgcc gacagtcgat tcgcgccagc
    35161 cgggcatggt ttcgtaaatc ggtttgcagg tttccaccgc atccgaaccg caaggcagga
    35221 tgtcggtttt gccgccgcct ggcaattcgt agccgacgca gatattgatg gtttcaacgc
    35281 cgtccattac atcgagtttg gtaatgcaca taccggaaat gccgttgatt tggatggagc
    35341 gtttcagggc ggcggcatca aaccagccgc agcggcgcgc gcggccggtt accgaaccga
    35401 attcgtgtcc gcgctccgcc aaacctgcgc ctacttcgtc gaacaattcg gtcgggaacg
    35461 gacccgaacc gacgcgcgtg gtataggctt tgacgatgcc caaaacataa tccagcattt
    35521 gaggacctac gcccgcgcct gccgaagccg cgccggcgag acagttggac gaggtaacga
    35581 aggggtaagt gccgtagtcg atgtccaaca acgcaccttg cgcgccttca aacagcagtt
    35641 tttcgccgtt tttgtttttt tcgttcaaca cgcgggacac gtcggcaatc atcggcgcaa
    35701 tgcgcggcgc gactttttcg ataaccgcca tcacgtcttc cgctttaacc ggtccggcgt
    35761 tgtgcaggta ttggagttgg acgttgtaat aggcaaggac ggcatccagt ttttcacgca
    35821 gtttttcagg atgcagcaaa tcggcggcgc gaatggcgcg gcgtgccact ttgtcttcgt
    35881 aggcagggcc gatgccgcgg ccggtcgtgc cgattttgcc tttgccgcgc gatgcttcgc
    35941 gggcttggtc gagcgcgatg tggtaaggca ggatcagcgg gcaggtcggc gcgattttca
    36001 gacggccttc gacgtttttc acgcctgccg cgttcaactc gtcaatttcg cccaacaggg
    36061 cttcggggga aacgacaacg cccgaaccga tgaagcaatc caatccttcg tgcaggatac
    36121 cgctcggaat caggcgcaaa atggtttttt tgccgccgac gaccaaggta tggcccgcat
    36181 tgtggccgcc ttggaagcgc acgacgccgc cggcttcttc cgccagccag tcaacgattt
    36241 tacctttacc ctcgtcgccc cactgtgcgc cgattactac aacattttta gccatagcca
    36301 tataacctat cgatattaaa aattattgcg gaaaatcccc aaaccggtgc ggctttgcgc
    36361 cccgccccgg tttgcctttc atccgctatg aacgggtatt tagcgtttca cgatctgcca
    36421 aacgccgtcc gtctttttca gacggcctgc aagctcttcc gaaacattgt gtccgatacc
    36481 gtagtcaatc acgacacact gcccttgttc acgcaaggct tcgaccgctt cgcgcgccgc
    36541 ttctgcatct tccgcatcga ccaacacggc gggctgccgt tcgacggcgg gcaaacgccc
    36601 gataaagctg cgtaagtcga aactgaatcc cgttgccggg cgcgccctgc cgaaatatcc
    36661 gcccaatccg tcataacgcc cgccgcgcgc gaccgcgtcg tggaaatcgg cggcataggc
    36721 ggcatacagc aagcccgtgt ggtaattgtc gacgcgcagc tcggacaagt cgatatggat
    36781 ttcattatcg gggaatgcgt cgcacaccgc ctgcaattcg tccaacgcgc cgccgaccgc
    36841 cgacaaatcc ggcaaccgtc cgcgcgcgtc ggacaacact tcacgcccgc cgtacaggcg
    36901 cggcagcaac gagaatgctt ttgcccacat accgtccagc ttccaagcct tgacctgcgc
    36961 ttcgaccgac ccggtatctt tatcctgcat caaggcaagc agcgttgcgg actgccccgc
    37021 atccaaatgc gccgcatcgg acaaggcgcg gaatatgccg atatgcccca gcgaaagcag
    37081 cactttgccc atatcggcaa ttttcatgct tttcagcata aggtctatca gctcgatgtc
    37141 gccacggatg tcggcaaaac cgtacatttc cgcccctgcc tgcaagggtt cgcgcatatt
    37201 cggcagaccg tcgggctgcg cgtgcaacac cggaccggca taacacaacc ggttaatccc
    37261 ttggttggcg gacaaaagat gggcatcgat acgcgccacc tgcggcgtga tgtcggcgcg
    37321 tatgcccaac tgcctgccgc tgagcctgtc cgttaccaaa atggttttca gtgaaagccc
    37381 cgcatcgata tgcgtcagca gggaatgtgc gtactccatc agcggaggct gtaccagttc
    37441 ataaccgtgt acgcggaaca gtgccaacaa ctgctcgcgc gcgctttcaa gctgccgcgc
    37501 gttcgtgggc agtacgtcgg cgatatgttc cggaagctgc catgtctgca tgagaatctg
    37561 ccttctgttt tatcctgtaa acacaaaaga ctgcccgatc cgcaatcaga cggcatcttt
    37621 gccgcaacgc atcacgccgc tgtcaaaacg gcgatgcttc cttccgattc agaatccgaa
    37681 cagcccctgt ccgacggctc aatgccgtca agtcgttaaa accaaacttt accataaaat
    37741 acacacaatc tgaatatact tttatacccc gtatccgccg tctgacggca cggtattttt
    37801 tactatataa tgtgcaccat tatttctcat cacttcccgc tgccgttttt ttgtttcaga
    37861 cggcattttt attgactgaa cacttatgct gctcgacctc gaccgctttt ccttttccgt
    37921 cttcctgaaa gaaatccgcc tgctgaccgc ccttgccctg cccatgctgt tggcgcaggt
    37981 cgcgcaggtg ggcatcggtt tcgtcgatac cgtgatggcg ggcggtgcgg gcaaggaaga
    38041 tttggcggcg gtggctttgg gcagcagcgc gtttgccacg gtttatatta cctttatggg
    38101 cattatggcg gcgctgaacc cgatgattgc ccagctttac ggcgcgggta aaaccggtga
    38161 agcaggcgaa acggggcggc aggggatttg gttcgggctg attttgggga ttttcggcat
    38221 gattttgatg tgggcggcga ttacgccgtt ccgcaactgg ctgactttga gcgattatgt
    38281 ggaaggcaca atggcgcagt atatgctgtt caccagcttg gcgatgccgg cggcaatggt
    38341 acaccgcgca ctgcacgcct acgcttccag cctgaaccgc ccgcgcctga ttatgttggt
    38401 cagctttgcg gcgtttgtgt tgaacgtgcc gctgaactat attttcgttt acggcaaatt
    38461 cggtatgccc gctttgggtg gcgcaggttg cggcgtggcg acaatggcgg tgttttggtt
    38521 cagcgcgctg gcattgtgga tttatatcgc caaggaaaaa ttcttccgcc cgttcggact
    38581 gacagcgaaa ttcggcaaac cggattgggc ggtgttcaaa cagatttgga aaatcggcgc
    38641 gcccatcggg ctgtcttatt ttttggaagc cagcgcgttt tcgtttatcg tgtttttgat
    38701 tgcgcctttc ggcgaggatt atgtggcggc gcagcaggtc ggcatcagtt tgtcggggat
    38761 tctctatatg attccgcaaa gcgtcggctc ggcagggacg gtgcgcatcg gcttttcgct
    38821 tgggcggcgc gaattttcgc gggcgcgtta tatttcagga gtgtcgctgg tgtcgggctg
    38881 ggtgctcgcc gtgattaccg tgctttcctt ggtattattc cgttcgccgc tggcaagcat
    38941 gtacaacgat gatccggcag ttttaagcat cgcctccacc gtcctgctgt tcgccggcct
    39001 gttccaaccg gcagacttca cccaatgtat cgcgtcctat gccctgcgcg gctacaaagt
    39061 caccaaggtg ccgatgttca tccacgccgc cgccttctgg ggctgcggcc tgctgccggg
    39121 ctatctgctc gcctaccgtt tcgatatggg catttacggc ttctggacgg cattgattgc
    39181 ctcgctcacc atcgcagccg tcgccttggt gtggtgcttg gaaaaataca gtatggagtt
    39241 ggtcaaatca cacaaggccg tctgaaaacg cagcacactt caattcaaat acagttaaag
    39301 ctcaaaccat gcaacccatc cgataccgaa ccgacctcac cccctacaac actttcggcc
    39361 tgcacgccca agcccgagcc tttatcgcgc ttaaacacgc cgacgaattg cgcgacatcg
    39421 tccgactgcc cgagttcgac cgcgatactg ttttatggct gggcggcggc agcaacatcc
    39481 ttttgatgca ggattacgac ggcttggtcg tccatatgga aaacaagggc atacgcgaga
    39541 ttgcgcgctc agacggcatg gttctgattg aagcgcaggc gggcgaaatt tggcacgatt
    39601 tcgtgttgca caccgtcgcg ctgggtttga gcggtttgga aaacctgagc ctgattccgg
    39661 gtacggtcgg cgcgtcgccc gtgcagaaca tcggcgcata cggcgtggag gcgaaagatg
    39721 tgattcacag cgtgcgctgc ttcgatttgg atacggaaac ctttgtaacc ctttccaatg
    39781 ccgactgccg cttcgcctac cgcgaaagcc tgttcaagca ggaaggcaaa gggcgttatg
    39841 tgattgtttc ggtcgtattt gcattaaaaa cgcattttgt gccgaacttg ggctacggcg
    39901 atttggcggc gaaggttgcc gaattgagtg caggtaggga ggcgacggcg aaagacgttt
    39961 ccgatgcggt atccgccatc cgcaacagca aattacccga cccgaaagtt ttgggcaatg
    40021 tcggcagttt ctttaaaaac cccgtcgtca gcgcggaaaa agccgccacc ctgttgcagc
    40081 ggcatcccga tatgccgcgc tatccgcagc ccgacggttc ggtcaaactc gccgccggct
    40141 ggctgatcga ccaatgccgt ctgaagggct tccaaatcgg cggtgcggcg gtacatgacc
    40201 ggcaggctct ggttttggcg aacaaaaaca atgcctcttc agacgacatc tggaaactgg
    40261 cgcaacacgt ctgcaataca gtatttactc gatttcaggt agaattacac gccgaaccca
    40321 attggctgcc cgcttcgttc agcctgtaaa tccaaggagt atgcccgtga cccgcattgc
    40381 aaaaaccaat acttacaccc gcatcatcga cgccagcctt gcgcttttca acgaggaagg
    40441 cgagcgcaac atcagcacca accatattgc cgcccacttg ggcatcagtc cgggcaatct
    40501 ctattaccac ttccgcaaca aagacgaaat catcgtccaa ctgttcaaac gttacagcga
    40561 agccctgctg gaatacctca acgaagccgt gttgccctct gatgtggaag actctatcaa
    40621 ctatatggca ggcatttacg atgtcatgtg ggaataccgc ttcctcttta gcgacgtaaa
    40681 caccctgctt gcgcgcagtg ccgaattgtt gggcgaacac aataccttta cccaagccaa
    40741 agtctcgccg cttctagtca aactgctgac ccagctcaac gggctgaacg tcatcaacgc
    40801 cgaccaaacc gctatgaacg acctcgccgt caatatgtgg atggttacga aatactggtt
    40861 cgacttcgac agttccctgc gcggccgtac caagctgacc gaagactcca aagcacgcgg
    40921 cgtccgtcgt accttaagcc tccttcgccc ctatcttttg cccgaacacc gcgcggaata
    40981 cgaccgcaaa atcagcaacg gcaacccgta aacccataac gtccgaatca gccttcagac
    41041 ggcacttacc cgacggtatc agcgaatgcg cgcagtcccc ccgccccata tctccatcac
    41101 aggcctaggt tacctcggcc tgccgctggc acaaaagttt taccgacacg gcagccgcgt
    41161 cgccgccata aaacgcagcc tgacttcgga cgacatcaac ctgcccatac acctcgacac
    41221 cttcgacctc aaccgtaccg atacctttca gacggcattg tggcggcatc acgccgacaa
    41281 acccgtctgg ttcttcctgc tgccgccctc ctttttggcg cattacgccg acaccgtcaa
    41341 acaatgggca gagcttgccc gggcgtgcaa cgtgcaacac ctgattttca caagcagcac
    41401 aagcgtttac ggcgataaag cgcgagaatg caacgaaacc gccgcacccg atccgcaaac
    41461 cgagtccgcc cgacaaatcc ttgccgccga acaacacctg ctcgacaacg gcgttccgaa
    41521 catcgacatc ctgcggctgg gcgggcttta ttgcgccgaa cgccatcccg tcagccgcct
    41581 cgtgcaaaag caaaacatcc aaggcggcaa ccggcctgtc aatatcgtcc accgcgacat
    41641 cgccgtcgaa aacctgtttc agacggcatc caaccccggc ggcaggcggc tgaaaaacat
    41701 tatcgaaccg cgccacccga cacgccgcga attttatacg gaagaagccg ccaaactcgg
    41761 cctgcccgcg ccggatttct cacccgacga cagcgtgggc aaaatcatcc gtaccgtttg
    41821 cgataacggc ttaagcctgt aaaataagcg gcaacagcaa caacaggcat tctccgcccc
    41881 aatcagaaaa tatgtccgcc ctcctcccca tcatcaaccg cctgattctg caaagcccgg
    41941 acagccgctc ggaacttacc tcctttgcag gcaaaacact gaccctgaac attgccgggc
    42001 tgaaactggc gggacgcatc acagaagacg gtttgctctc ggcgggaaac ggctttgcag
    42061 acaccgaaat taccttccgc aacagcgcga tacggaaaat cctccaaggc ggcgaacccg
    42121 gggctggcga catcaggctc gaaggcgacc tcatcctcgg catcgcggta ctgtccctgc
    42181 tcggcagcct gcgttcccgc gcatcggacg aattggcacg gattttcggc acgcaggcag
    42241 gcatcggcag ccgtgccacc gacatcggac acggcatcaa acaaatcggc aggaacatcg
    42301 ccgaacaaat cggcggattt tcccgcgaac ccgagtccgc aaacaccggc aacgaagccc
    42361 ttgccgactg cctcgacgaa ataagcagac tgcgcgacgg cgtggaacgc ctcaacgaac
    42421 gcctcgacag gctcgaacgc gacatttgga tagactaacc ttcagacggc atccgacatg
    42481 aacagccctt ttcacaacat cggtatcgta acccgcccca acacgcccga catccaagat
    42541 accgcacaca cgctgattac ctttttgaag cagcacggct ttaccgtcta tctcgacgaa
    42601 gtcggcgtaa gggaatgctg catctatacc caagacaccg acggctgcca tatcgtcaac
    42661 aagaccgaac tggggcaata ctgcgacctg gtcgccgtct taggcggaga cggcaccttt
    42721 ctctctgccg cccgcgaaat caccccccgc gccgttccga ttatcggcat caaccaaggt
    42781 catttgggct ttcttaccca aattccccgc gaatatatga cggacaagct attgcccgtt
    42841 ttagaaggaa aataccttgc cgaagagcgc atcctgattg aagccgcact gatccgcgaa
    42901 ggcaaaaccg ccgaacgcgc cctcgccctc aacgatgccg tcctctcccg cggcggtgcc
    42961 ggacagatga ttgaatttga agtcttcgtc aatcaggaat tcgtctatac acaacgttcg
    43021 gacgggctga ttgtctccac ccccaccgga tcgaccgcct attcgcttgc cgccggcggc
    43081 ccaatcatgc aggcaggatt acacgccttc acgctcgtcc ccatctgccc gcaatctatg
    43141 actaaccgcc ccatcgccat tccagacacg tccgaaatcg aaatcctcgt tacccaaggc
    43201 ggcgacgcgc gcgttcattt cgacggacaa tccttcatcg acgtgcaaaa cctcgaccgc
    43261 atcatcatcc gccgctacca caatccctta cgcatcctac acccgaccga ctaccaatat
    43321 ttcaaaaccc tgcgccaaaa actgcactgg ggcgagcaat tagtctaagc cggccttcac
    43381 ccgaaagtac ccatgaattc caaaatttcc tccgaccata cagaagatac catacgcctc
    43441 tccaaacgta tggcacaact ggggctttgt tcgcgccgcg aagccgacgg ccatatcgaa
    43501 cagggctggg taacggtcaa cggcaaaacc gccgtactcg gtcagaaagt ttcaccggca
    43561 gaccgtatcg aactgaacaa gaaagcccac gaacagcagg cggcacgcat taccatcctg
    43621 ctgaacaaac ccgtcggcta tgtcagcgca caagcggaaa aaggctataa atccgccgcc
    43681 gaactgatta cccctgaaaa ttgctgggaa ggcgacacca gccgcatcag tttcgatccg
    43741 aaacacaaaa tcggtcttgc ccccgccggc aggctcgata tcgattcagt cggattgctg
    43801 gtgttgacgc aagacggccg tatcgccaag cagcttatcg gcgaaaacag cggcagtgaa
    43861 aaagaatatt tggtgcgcgt gcgcggcaaa ttggacgaaa aaggacttgc cttactgaat
    43921 cacggattga gtttggacgg tgataaactg cgtcccgcca aagtagaatg gcaaaatgaa
    43981 gaccaactgc gcttcgtgtt gaaacagggt aaaaagcggc aaatccgccg tatgtgcgaa
    44041 ctggtcggac tgcgcgtcgt cgggctgaaa cgcatccgca tgggcaaggt caaactcggc
    44101 aggctgccgc ccggcaaatg gcgttatctc gctcccggcg aatcgtttta aataagcatt
    44161 aaaaaatgcc gtctgaaaat ccttgcgttt tcagacggca ctgccttatg cccaaataac
    44221 ggtttcttcc aagggcctgc gcgctttcgt gacgatttcc tgaacccggt agccgaatat
    44281 cgcggcaacg gacacgcccc attctgccgc atcgaacaaa ccgaactgcc cggacaatac
    44341 gcgttccata tcggcatagt tgaaaccttc caccgggcag gaatcgatac ccgccatcgc
    44401 cgcacccgtc atcatgttgg ctaacgcgat ataggtctgg cggcatcacc agtcaaacaa
    44461 ggcgcgcgaa tcgtccaaaa tcttgatgtc gtcagcttga aacgcctgat acctcgccaa
    44521 agatttttct acggcatccg gttcggtaac gccgcgccgt ttgagacttt ccaacataaa
    44581 cgggctgtcg aagcgggcat ttttcttcgc caaaaacacc accaaatgac tggcggtatc
    44641 caaagcatcc gccatacccc aagaaaacag cttgattgcc tgtcggattt cggggttttg
    44701 aaccacaaca aaccgccaag gctccgaacc gaccgaactg ggcgacaaac gcccgagttc
    44761 taaaataaac tgaaaatcct cggcactgat tttacgtgcg catcgtaatg ccggcacgat
    44821 ttgcggtttt taaatgcgga tagaacctgc tctttgctta atactgtcat cgtgcatcct
    44881 tttattactt tgattgcaaa ttgatttcaa accgatacgg cactgccgca ccctataagc
    44941 cgtccccgcc atcaagcttc acccggattt tacccatctc tctacggctg ggaaaacaaa
    45001 gcggcggcag attcgggaat aactccccaa tgcctttcag acggcatttg gacatcgcaa
    45061 acgcgcctac gggtaggcga tataggcata agccgaatgg ttgtggatag actcgaaatt
    45121 ctcgctctcg acgacgaaac tcttgatgcg tttgtcggca atcagcgaag tagcgacatc
    45181 gcgcaccata tcttccacga atttcgggtt ttcgtaggcc ttttcggtaa cgtatttttc
    45241 atcggggcgt ttgagcaggc cgtagagttg gcagctcgcc tgcgtttcca cataatcgat
    45301 aacttcctcg ataccgactt cggcatcgga agtcaggctg acggtaacgt gcgaacgctg
    45361 attatgcgcg ccatattggg aaatttcttt ggaacacggg caaagcgagg ttacgggaat
    45421 catgaccttc atactgtggc cgtatgcccc gtctttcatt tcacccgtga ggctgacatc
    45481 ataatccagt aaggaccgga taccggatac cggcgcggtt ttcttgcgga aaaacggaaa
    45541 agaaacgctg attttgccgg cgcgggaatc caaaagcgcg accatttcgg cagtcagcct
    45601 atgcaattgt gcgaaatcca agacttcggt atgttgctcc atcaatgcga caaagcgcga
    45661 catatgcgtc cctttctgct cggcgggcag ataaaccgtc atcgtcaggc gagcaaccgt
    45721 ggattgtgtt ccttcggcgg ttttcagggt aatcgggaag cgcaggtctt taatgccgac
    45781 ctgattgatc ggcaggttgc gcaaatctct gctggattgc acgtctgcaa tggcgttcat
    45841 gcgttgtttg tccttaatat tcagataatt attgagatgt gtgtattgta tggcaggcag
    45901 atgccgtctg aaaaaacgct gtcggccgtc ctgcctgcaa atgcgggatt atatcacttg
    45961 cttttggcgg ctgcattgat tgtttctatt ttcgggatag tgataaaatc ccgacttatc
    46021 catatcttac aaggcagaaa cagatgaaat tcccgacccg tgccatccat tccggctacg
    46081 attgcgacga acacaaccgc gcgctgatgc cgccgattta tcaaaacagt atgtttgcgc
    46141 tgcacgaaat cggcgaaaat gtgccttacc gttattcgcg cctgagcaac ccgacccgtc
    46201 agattttgga agacaccgtt gccgatttgg aacacggtgc ggcaggtttt gcgttttcca
    46261 gcggtatggc ggggattgat gccgtgtggc gtaccttcct gcgcccgggc gataccatcg
    46321 tcgcggtcgc cgatatttac ggcggcgcat atgacttatt ggtcgatgtt tatcaaaaat
    46381 ggggggtgaa cgttgttttt gccgatttgg gcaatcccga taatttggac gaattgctca
    46441 aagcgcacaa tgtcaaactg gtttggctgg aaatgccgtc caatccgctt ttacgcttgg
    46501 tagacatcaa agcccttgca gcgaaagcca aagcagccga cgcgctggtc ggcatcgaca
    46561 acacctttgc cacgccgtat ctgcaacagc ccttggatat gggttgcgat tttgcattcc
    46621 attccgctac caaatatttg tgcggccatt ccgacgtatt gatgggcatc gtcgttgcca
    46681 aaaccaaaga gctggctcag cctttgcacg atatgatggt gcataccggc gcgattgcag
    46741 gtccgacgga ctgctggctg gtgttgcgcg gcatcaaaac gcttgccctg cgtatggagg
    46801 cacactgcaa aaacgcactc gaaattgcgc gccgtttgga agcccatcct gccattgaaa
    46861 aagtgttcca tcccggcctg ccgtctcacg aacattacgc actcgcccaa gcgcaaatgc
    46921 ccaaaggcat cggcggcgtg gttacggttt atctcaaaaa cgacacgcgc gaagcggcaa
    46981 acagcgtgat taaaaacatg aaactggtca aaatgacctc cagcctcggc ggcgtggaaa
    47041 gtctggtcaa ccattgctat tcccaatctc acagcggcgt gccgcataat gtgaaaatgg
    47101 aaatgggcat cagggtcgga ctcctgcgct tctccatcgg tattgaagac gtagatgata
    47161 tttggaacga tatttccgcc gcactcgata caactttgta aaccgtaaaa aatgccgtct
    47221 gaaaccatag tttcagacgg catttcaata ttgcgaatca aatatcaacg cttcagcatc
    47281 tttgccgcct caatggcgta ataggtcaaa atcccgtccg cacccgcacg tttgaatgcc
    47341 agcaggcttt ccaaaaccac tttgccgccg tccagccagc cgttggcaac cgccgcctgc
    47401 aacatcgcat attcgcccga aacctgatag gcataagtcg gtacgccgaa ctcgtccttc
    47461 acgcggcgga caacgtccaa atacggcaaa ccgggcttca ccatcaccat atccgcacct
    47521 tcctgaatat cgagcgccac ttcatgcagc gcctcatcgg tatttgcagg atccatctga
    47581 taggtctttt tatctgcctt tcccaaattg cccgaactgc ctaccgcatc acggaaaggg
    47641 ccgtagaatg cagaagcata tttggcggaa tatgccataa tccgcgtatg gatatgtccg
    47701 gcatcctcca aagcctcgcg gatggcgccg atacgcccgt ccatcatatc ggaaggagca
    47761 acgacctgcg tgcccgcctc tgcatgacat aaagcctgtt tcaccaagac ttctacggtt
    47821 tcatcattca tcacgtaacc gttttcgtcc gtcagtccgt cctgaccgtg caccgtataa
    47881 ggatcgagcg cgacatccgt cataatgccc agttcgggaa acctctcgcg caaggctcgg
    47941 acagttgacg gcacgagtcc ttcgggattg tacgcctcct gcgcacgccc ggttttgttt
    48001 gccgtaacca cgggaaagag tgccaacatc ggaataccga gcttcaccgc ctcttccgcc
    48061 gtaaacagca gcctgtccaa actctgacgc ttcacgcccg gcatagaagg cacatcctcc
    48121 tcgcgcgccg ccccctccaa tacgaacacc ggataaatca aatcatcggc ggtcagcata
    48181 tgctcgcgca tcaggcggcg tgaaaaatca tccctgcgca tacggcgcat acgcgaagcc
    48241 ggaacattgc ggtaaggaaa ttgcataagc cctccaatca tcctgtcaac aattcaaatc
    48301 atacggaagc cgccgcaact tgcagacggc atcgagcggt aatcaaaatc cgttttcacc
    48361 ggaaagggtt tcggcacgcc gaaatgccgc gccgaaggac agcgtgccgc ccattgaaag
    48421 aacaatagca aaacaaccac ttcaggctgt caggcctcga tattcttacg ccacaatacc
    48481 aaaagtttcc cgatatgccg aacaggttgc gcgtcaaccg cctcacacaa ggtattgcag
    48541 atttcgatac gctcggcacg gtcgtcgccg aatacgcgca ctttaatcag ctcatgcgcc
    48601 gtcagtgccg catcggtttc cttgatgact gcgtccgtca gaccctgctg accgaccatc
    48661 acaacaggat ggagatggtg cgcgcgcgct ttcaattcca aaatttcttt ggtgttcaat
    48721 ttggtatccg ccattttcct actgtttgaa taaaaaggat aatgcagcat tgtacgcgat
    48781 ttggcacggc aacggcaaaa ttaacgtaca atacaccgtt tacattccga cccgcgaacc
    48841 actatggctg tacgttccaa atcctcaaaa gcgtggctgc acgaacacat caacgaccag
    48901 tacgtccata tggcgcaaaa agacggctac cgcgcccgtg ccgcatacaa acttttggaa
    48961 atcaacgaaa aagacaagat aatcaaaccc ggcacggtac ttgccgactt gggcagcgcg
    49021 ccgggaagct ggtcgcaggt tgccgccaag ctgacgggta cttccggagc ggttttcgcc
    49081 ttggacatcc tgcctatgga agccataggg ggcgtgtcct tcattcaggg cgacttccgc
    49141 gagaacgacg ttttggcgca attcgaaacc ttgttggaca accgcccgct cgaccttgta
    49201 atctgcgata tggcgcccaa tatgtcggga aacgccgtaa gcgatcaggc acgcagcttt
    49261 tatctgtgcg aactggcttt ggacttcgcc tcgcaacacc tgaaaaccgg cggcagcttt
    49321 ttggtcaaag tctttcaggg tgcaggctat caggaataca tggcagccat gcgcgaaatt
    49381 ttcggcactg tgcagacgcg caaacccgaa gcctcgcgca atcgctccag tgagatttat
    49441 ttattgggca aaaataaacg ctgacaatac agacggcgtg ctttacaatc atttccgttt
    49501 tacacctcaa ttatggagcc ttgctaagtg gggaacacct ttaaatcaat ccttgtctgg
    49561 gtcgccttgg gtatcggcct gatggctgcg ttcaacgctt tggacggcaa aaaagaagac
    49621 aacgggcaaa tcgaatattc tcagttcatc cgacaggtca acaacggcga agtatccggc
    49681 gtcaacatcg aaggatccgt cgtcagcggc tacctgatta agggcgagcg caccgacaaa
    49741 agcaccttct ttaccaacgc gcctttggac gacaacctga ttaaaacact gctcgacaaa
    49801 aacgtccgcg taaaagtaac gccggaagaa aaaccgagcg cgctggctgc cctgttttac
    49861 agcctgctgc ccgtcctgct gctgattggc gcgtggttct actttatgcg tatgcagacg
    49921 ggcggcggcg gaaaaggcgg cgcattctca ttcggcaaaa gccgcgcacg cctgctggac
    49981 aaagatgcca acaaagtgac ctttgccgat gtcgccggct gcgacgaagc caaagaagaa
    50041 gtgcaggaaa tcgtcgatta cctcaaagca ccgaaccgct atcaaagctt gggcgggcgc
    50101 gtgccgcgcg gcatcctgct ggcgggcagc ccgggtacgg gtaagacgct tttggcaaaa
    50161 gccattgcag gcgaagccgg cgtgccgttc ttcagcattt ccggttccga ctttgtcgaa
    50221 atgttcgtcg gtgttggtgc gagccgcgtc cgcgatatgt tcgagcaggc gaagaaaaac
    50281 gccccctgca tcatctttat tgatgaaatc gacgcagtcg gccgccaacg cggcgcaggt
    50341 ttgggcggcg gcaatgatga gcgcgagcaa acattaaacc aattattggt tgaaatggac
    50401 ggttttgaga gcaatcagac tgtaattgtg attgcggcaa ccaaccgccc cgacgtactc
    50461 gatcctgcgc tgcaacgccc cggccgcttc gaccgccaag tcgtcgtccc cctgccggac
    50521 atccgggggc gcgaacagat tttgaacgtc cattctaaaa aagtgccttt ggacgaatct
    50581 gtggatttat tgtccctcgc gcgcggcacg cccggttttt ccggcgcgga tttggcgaac
    50641 ctggtcaacg aagccgccct gtttgccggc cgccgcaaca aagtcaaagt cgatcaaagc
    50701 gattttgaag acgccaaaga caaaatctat atgggtccgg aacgccgcag tatggtgatg
    50761 cacgaagacg aaaaacgtgc gacggcgtat catgagtccg gacacgcgat tgttgccgaa
    50821 agcctgccct ttaccgaccc cgtccacaaa gtaaccatta tgccgcgcgg acgtgcgctg
    50881 ggtctgactt ggcagcttcc ggagcgcgac cgcatcagta tgtataaaga tcagatgttg
    50941 agccagctct ccatcctgtt cggcggacgg attgccgaag acatcttcgt cggacgcatc
    51001 tccaccggtg catcaaacga ctttgaacgc gcaacccaaa tggcacgcga aatggtaacg
    51061 cgctacggca tgagcgacaa aatgggcgtg atggtttatg cggaaaacga aggcgaagtc
    51121 ttcttggggc gcagcgtaac ccgttcgcaa aacatttccg agaaaaccca gcaggacatc
    51181 gacgcggaaa tccgccggat tttggacgag cagtatcagg ttgcctacaa aatcctcgat
    51241 gaaaaccgcg acaagatgga aacgatgtgc aaagccctga tggaatggga aaccatcgac
    51301 cgcgatcagg tactggaaat tatggcgggc aaacaaccca gcccgcccaa ggattacagc
    51361 cacaacctgc gcgagaatgc ggacgcggcg gaagataacg cgccgcacgc tccgactcgg
    51421 gaaaaaaccg aagcacctgc cccggcagac accgcctcga cagagtccgg gcagcagcct
    51481 gaaaacaagg cttaacttcc cgaacaaacg gcagcccgca agctgccgtt ttcccatccc
    51541 gttttcagac ggcattttcc gctcaatgcc gtctgaaacg ccaaagccgt atcggttttt
    51601 gcgaatctgc gatatggcgg tttaaattga aatcagaacg gggcaaggct gtaccggttt
    51661 aaaattaaac cgctataata ccgcccttcg aatacacgcc cgcattggtg atgcccgtga
    51721 aaaaagttga aaaaaacatc ttggtgctgc acggcgcgga caaaatgttc gagctggtcg
    51781 acaaggttga ggattacccg cactttctgc cgtggtacag caagaccgag gtcatcgggc
    51841 gtagcggcaa cgaactgaag gcgcggctgt ttatggatta tatgcgcgtt cgccaatcgt
    51901 ttgccacgca caaccgcaac atccccggca gggaaatccg tatggaactg ctcgaaggcc
    51961 cgttcaaaac cttacgtgga acgtggaaat ttatcgattt gggcgacgat atgtgcaaaa
    52021 tcgaattcaa tttggaatac gatttttcca atgccgtttt gtccgcctta atttcccccg
    52081 tcttcaacca cctttccgcc acgctggtcg aagcgttcgt caaagaggca gaccgccgtt
    52141 atgcttgaaa ttgagattgt gtacggtctg cccgaccgac aggttttgaa aaccatgcag
    52201 cttgccgagg gaacaaccgt ccgcaccgcc gcactgcaaa gcggtttgga cggcatattt
    52261 gaaaatttaa acctgcattc cgcgcctttg ggcattttcg gcaaagccgt caaagacgac
    52321 acgccgctgc gcgacggcga ccgcatcgaa gtgtaccgcc cgctgttgat cgaccccaaa
    52381 gaagcgcgcc gcaaacgcgt tcaaaatcaa gaagaataac catgccgtct gaagccttca
    52441 gacggcattc gacagaaaca cggaaaatat catgtcaaac acaatcaaaa tggttgtcgg
    52501 cttgggcaac ccgggcaaag aatacgaaca gacccgccac aatgcgggct tttggttcct
    52561 cgacgaactg gcgtggaaat ggaaggcttc gtttaaagaa gaaaaaaaat tcttcggcga
    52621 agttgcccgc gccgccctgc ccgacggcga tgtttggctg ctcaaaccgg ccacgttcat
    52681 gaaccgttcc ggacaggcgg ttgccgcgct tgcacagttc tacaaaatca aacccgaaga
    52741 aatcctcgtc gtccacgacg aactcgacat cccttgcgga cggatcaaat tcaaactcgg
    52801 cggcggcaac ggcggacaca acggcttgaa agacattcag gcaaaactcg gcacggcaga
    52861 ctattaccgc ctgcgcctcg gcatcggcca ccccggcgac cgcaacctcg tcgtcggcta
    52921 cgtcttgaac aaaccgagcg cggaacaccg ccggcaaatc gacgatgccg tcgccaaatc
    52981 cctgcaggcc gtacccgaca tcatttccgg caaatgggaa gaggcaacgc gcttcctgca
    53041 cagcaaataa tccaatgccg tcttaagccc tttcagacgg cattttcccg atttccgtat
    53101 ccgaacagtc atgaccgaac tcaagcagct tatccaaacc gaatccatcc ccgtcatcga
    53161 agaaaccctc gatttcctgc tgtacgaatg cagcatcgac gacgcaccgt ccgccgaaga
    53221 agtggcacaa tggcgcgaca tacttgccgc acgcggcggc aaattcctgc gcctgtccaa
    53281 aatctgccaa acgtggctgg acgaggaggc ggcatgaagc tgccgcgcaa ccgcttcagc
    53341 ctgctttccg cattgtggtt tgccggcggc atctattcgc tgctcttcaa agctgccgac
    53401 accgcgccgc cgccgtttcc acatttcgac aaagcagcac accttgccct gtttttcgca
    53461 caaatcttgc ttctggccaa agcattcaaa accggaaaac ttcccatccc ctaccgcagc
    53521 ctgattgcgt tcgccttctg ttttgccgtc ggcagcgaat gcgcgcaggc atggtttact
    53581 gcaacgcgaa ccggcagttt gggcgatgtc cttgccgacc tgacgggcgc agcccttgcc
    53641 ctctttgccg cgcgttctgc ctgccgcccg gactaaatcg gtttatttct cccaaacagg
    53701 atgaatcccc ctgatgcatc ctgtttttcc ccttacatat caatcccatc tcataaacaa
    53761 aaccgacaaa tcgtttacaa tatatttaca ctacaccgaa ttacaaatat actcgaagcg
    53821 gttgcagaac cgacggtttc gcaaaccgtt tcggattgca cgacacaaaa gaaaaaccga
    53881 ttttgttgcc ttaaaggagc attcatgaac ctgcatgcaa aggacaaaac ccagcatccc
    53941 gaaaacgtcg agctgctcag tgcgcagaag ccgattaccg actttaaggg tttgctgacc
    54001 accattattt ccgccgtcgt ctgtttcggc atttaccaca tcctgcctta cagccccgat
    54061 gccaataaag gtatcgcgct gctgattttc gttgccgcac tttggtttac cgaagccgtc
    54121 cacattacca taaccgcact gatggtgccg attctcgccg tcgtactcgg tttccccgac
    54181 atggacatca aaaaggcgat ggctgatttt tccaacccga ttatctacat ttttttcggc
    54241 ggcttcgcgc ttgccaccgc cctgcatatg cagcggctgg accgtaaaat cgccgtcagc
    54301 ctgttgcgcc tgtcgcgcgg caatatgaaa gtggcggttt tgatgttgtt cctcgttact
    54361 gcctttctgt ccatgtggat cagcaacacc gccaccgccg cgatgatgct gcctctagca
    54421 atgggtatgc tgagccacct cgaccgggaa aaagaacaca aaacctacgt cttcctcctg
    54481 ctcggcatcg cctattgcgc cagcatcggc ggcttgggca cgctcgtcgg ctcgccgccc
    54541 aacctgattg ccgccaaagc cctaaatctg gacttcgtcg gctggatgaa gctcggcctg
    54601 ccgatgatgc tgttgattct gcccttgatg ctgctctccc tgtacgtcat cctcaaacct
    54661 aatttgaacg aacgcgtgga aatcaaagcc gaatccatcc cttggacgct gcaccgcgtc
    54721 atcgcgctgt tgattttcct tgccacagcc gccgcgtgga tattcggctc caaaatcaaa
    54781 accgccttcg gcatttccaa tcccgacacc gttatcgccc tgagtgccgc cgtcgccgtc
    54841 gtcgtcttcg gcgtggcgca atggaaggaa gtcgcccgca ataccgactg gggcgtgttg
    54901 atgctcttcg gcggcggcat cagcctgagc acgctgttga aaacatccgg cgcgtccgaa
    54961 gccttgggac agcaggttgc cgccaccttt tccggcgcgc ccgcattttt ggtgatactc
    55021 atcgtcgccg ccttcattat ttttctgacc gagttcacca gcaacaccgc ctccgccgcc
    55081 ctgcttgtac cgattttctc cggcatcgct atgcagatgg ggctgcccga acaagtcttg
    55141 gtattcgtca tcggcatcgc cgcatcttgc gccttcatgc tgccggttgc cacgccgcct
    55201 aacgcgattg tgttcggcac gggcttaatc aaacaacgag aaatgatgaa tgtcggcata
    55261 ctgctgaaca tcctatgcgt agtattggtt gctctgtggg cttatgctgt actgatgtaa
    55321 accatcgacc taaacaacaa gaccgtctga aagaatattt ttcagacgac cttgaagttt
    55381 gtcgttcaga cacaatttgt cgaataattc aaaaccggat tctaacgaaa ggaaaaccca
    55441 tgattatcct gcacaccaac aaaggcgaca tcaaaatcga actcgatttc gacaaagccc
    55501 ctgttaccgc caaaaacttc gagcaatacg tcaaagacgg cttctacgac ggcgtaatct
    55561 tccaccgcgt catcaaaggc ttcatgattc aaggcggcgg catggatgaa aacatgaacg
    55621 aaaaagaaac ccgcgatccg attcaaaacg aagcgtccaa cggcctgccc aacgataaat
    55681 acaccatcgc catggcgcgc acttccgacc cccattccgc cggcgcgcaa ttcttcatca
    55741 acactgccga caacgctttc ctaaacttcc gttctaaaga gctgtacggc aaaaccgtcg
    55801 tccaagactg gggctatgcc gtattcggta aagtcgttga cggttttgac gttgtcgatg
    55861 ccatcgaaag cgtatccacc aaacgccacg gctaccacga cgatgtgccg accgaacctg
    55921 tcatcatcat taaagccgaa gcagtataaa ccggcaatcc gaaagcagcc tgcataaaag
    55981 gctgctttta tttcagacgg catgttattt gatgtaaccg ggctatgccg tctgaaaacc
    56041 ggacgcaagc cttcagacgg cataccgcta tgttaaaata cgcccgtttt tcctaaacgg
    56101 acacaaagga aaccttatgg caagcatcac ccgcgacatc ttcaaagcct acgacatccg
    56161 tggcatcgtc ggcaaaaccc tgaccgacga tgccgcttat ttcatcggca gggccatcgc
    56221 cgccaaagcc gccgaaaaag gtatcgcccg catcgcgctc ggacgcgacg gacgcttgag
    56281 cggccccgaa ctgatggagc acatccaacg cggcctgacc gacagcggta tcggcgtact
    56341 caatgtcggc atggttgcca ctcctatgct ctacttcgca gccgtcaacg aatgcggcgg
    56401 cagcggagtg atgattaccg gcagccacaa tccgcccgat tacaacggtt tcaaaatgat
    56461 gctcggcggc gacacgctcg caggcgaagc cattcaagaa cttttagcta ttgttgagaa
    56521 agacggtttt gttgccgccg acaaacaagg cagcgtaacc gaaaaagaca tctccggcgc
    56581 ataccacgac cacatcgtcg gacacgtcaa actcaaacgc ccgataaaca tcgccatcga
    56641 cgcgggcaac ggcgtgggcg gcgcgtttgc cggcaaactc tacaaaggtt tgggcaacga
    56701 agtgaccgaa cttttctgcg aagtggacgg caatttccct aatcaccacc ctgatccttc
    56761 caaaccggaa aacctgcaag atttgattgc cgcgctgaaa aacggcgatg ccgaaatcgg
    56821 cttggcgttt gacggcgatg ccgaccgctt gggcgtggtt accaaagacg gcaacattat
    56881 ttatcccgac cgccaactga tgctgttcgc ccaagacgtt ttgaaccgca atcccggcgc
    56941 gaaagtcatt ttcgatgtca aatccacacg cctgcttgcc ccgtggatta aagaacacgg
    57001 cggagaagcc ataatggaaa aaaccggcca cagcttcatc aaatccgcta tgaaaaaaac
    57061 cggtgcactg gttgccggcg aaatgagcgg acacgttttc tttaaagaac gctggttcgg
    57121 cttcgacgac ggcctgtatg ccggcgcacg cctcttggaa atcctgtccg cctccgacaa
    57181 tccgtccgaa gtgttggaca acctgccgca aagcatttcc acgcccgaac tcaacatctc
    57241 cctgcccgaa ggcagcaacg ggcatcaagt tatcgaagaa ctcgccgcca aagcccaatt
    57301 tgaaggcgca accgaaatca tcaccatcga cggcctgcgc gttgaatttc ccgacggctt
    57361 cggtctgatg cgtgcttcca ataccacgcc gattttggtg ttgcgttttg aagcggatac
    57421 gcaagcagcc atcgagcgca ttcaaaaccg attcaaagcc gtcatcgaaa gcaatccgca
    57481 tttaatctgg cctctgtaaa aatagaaaaa atgccgtctg aaactttcag acggcatttt
    57541 tcacaagtag ccaatcaaat cttggcagat tggatttggc ttaaaaatct ccgcgtccgt
    57601 tcgtgtttga ggtggtcgaa caactctttc gggctgccct gctctacgat aacgccgccg
    57661 tccatcacga cgacgttcgt ggcaacttcc agcgtgaact tgatttcgtg ggtaacgacg
    57721 accatcgtcc aaccttcccg cgccaattcc ttcatggcgt ccaacacgtc ttgcaccaac
    57781 tcggggtcca gcgcggaagt gggttcgtca aacagcatca attcaggctg aatcgccagt
    57841 gcgcgggcga taccgacacg ctgctgctga ccgccggaaa gctggtaggg atacaaatcc
    57901 actttatcgc ccaagccgac tttttccagc agtttcaaag cctcttcgcg cgcttgggcg
    57961 gcaggcttgc cctgtacggc aaccggccct tccatcacgt tttccaacac ggttttatgc
    58021 gggaagaggt tgtattgttg gaataccatt ccggacttgc ggcgcagtgc caaaatatcg
    58081 tgtttgcttg tttttttgga aaaatcaatg cgtaacggcc gcgcgttgtc gaactcgatt
    58141 tgtccgtctt cgggcatttc caacgcgttt aggcagcgca gaaatgttgt tttacccgag
    58201 ccggaaggcc cgaggatgac gaccacctgc cctttgccca catccaaatc gatgccgcgc
    58261 aaaatggtgt tttcgccaaa ggtcttatgg atattgcgga ttttaatcat gacaactcct
    58321 tatttggcga cataacggtc gaaacgtttt tccaaacgcg cctgaatcaa aaacagcact
    58381 ttacagaaac accaataaac caatgcagct tcgatataga caggcaaaaa gtcataagtg
    58441 cggtttgccg tttcctgtgc gacacggaaa agctccgtta ccgttaccac ggcggcaagc
    58501 gaggtgtttt tgaacaagcc gataaattcg ttgctcaacg gcggaacggc gacgcggaat
    58561 gcctgcggtg cgacgatgcg gcggaacgtc tgcatatagg tcataccgat ggagaaacct
    58621 gcttcccact gccctttcgg cacggacaaa atcgccgcgc gtatggtttc ggaagcgtat
    58681 gcgccgacat tgagcgaaaa gccgatgatg gcggcgggaa tcggattgat atagatgccg
    58741 acggacggca gcccgtaaaa cacaatcaca agctgaacca acagcggcgt accgcgaacg
    58801 acggaaatat aaaattccac cagcttcaac aagcattttt ggaaaatacc gccggaaggc
    58861 atgattctta ccaaagcaac ggctacggca ataatcatgc cgataacgaa agaagcgatc
    58921 gccaaaggca aagacactgt aaagccggct ttaaccatgg gccaaaacgc gctgatgagc
    58981 atatcagcgc gtgtttccgt cataaacggc agagaggcaa ggaaattatt gaacactgat
    59041 gtcttttccg aagaattgtt cgcccagttt tttcaacgtg ccgtcggctt tcagctcgtt
    59101 gattgccgtg ctgaatttcg ccacggcctc gtcattgccc ttgttgacaa tcagaccgga
    59161 accgactttt tcatcggcag gcgcggacca cacgattttc acccccgcat tcgggttttt
    59221 cttcagatag tccaaaaccg ccaattcatc gttcaacgtc gcatcggcgc gtttttgttc
    59281 aatcagggtc agcgattgcg ccaaaccgtc caccggcacg agttgcgcac ctgcggcttt
    59341 ggctttttcg ccgtagttgc tggtcaggga ttgcgcggtt ttcacgcctt tgatgtcggc
    59401 tatggattta atgttgctgt cgttatgcgc aaccaaaacc gcaccgctcc agctgtaagg
    59461 ttcggatttg tcaaatgtcg cctggcgttc ggggctggtc aggccgactt ggtttgccac
    59521 cacgtcgaaa cgtccggctt tcaaacccgc catcatcgaa tcccattgcg tttctttaaa
    59581 ctcgactttt acgcccagtt tttccgccac ggcgcgggtt acttccacgt cgtaaccggt
    59641 cagtttgccg tctttgtcgt ggtaggtaaa cggtgcgtaa gtgccttccg tgccgacggt
    59701 aaccgtgcct ttattgttga tgcgctcgat taaagaaccg gaaattgccg attgtgcagg
    59761 cgcggaagat gctccgctgc cgccttccga accgccgcag gccgccaaaa ccaatgcggc
    59821 aatgccgccg agtacgaatt ttttcaacat cattccctcc tgtaaaaagt gtgcaagtgc
    59881 ggcattctaa gtgaaattgc ggcaaacaca aagaatactt tttttatatg gttattccaa
    59941 atcggaatgg gaaagatatg ccgaagccgc cggaagcgga aataaagggg gcgggaaagg
    60001 cggcgggcgg cacgaaaccg aaaagcggca tcaggtatgc aaacggcgga ttcacgcacg
    60061 ccgttccggc aaaaatccgc ctgccgattc ggtgctataa tccacccgaa acagatgaca
    60121 ccaaaaagga acatctcatg aacacgcccc aatccgccat cattcccgac cacgcccaag
    60181 ccggcatctt tatcgaagcc gacttcgccg ccaaccgcct caacgatatt aaagccgcct
    60241 gccgcgcttc gctcgacgcg ttatccgcct tgaaagcccg ctttccggac gatattttgg
    60301 gtctgaccat cgccttcggc agcaaagctt gggcaacatt cggacacacg gacgaaggca
    60361 gtgaaatcaa acccttcccc gaaatgggca acgggcttgc gccgtccacg cagcacgata
    60421 tgtccatcca catccaatcc ttccgccaaa acgcagccta cgcgcttgcc caatccgttt
    60481 tgggcgcgtt cggcgacagc atatgcgtcg cgtccgaaga acacggtttg cgcctgtatc
    60541 aggatcgcgg gctggacggt ttcgtcgacg gcacggaaaa cccgcaaggc gatgaaacaa
    60601 tccgcgaagt cgccatcatc cccgaagggc tgcccgatgc aggcggcagc tatgtcctgc
    60661 tgcaaaaata cctgcacgat ttgaaaaaat gggatgccgt ccccgtcgcc gaacaggagg
    60721 cctcggtcgg acgcagcaag gaaaccgacg acgaattcag ccgcgatgtc cgcctgcccg
    60781 attcgcacct cggccgcgtc aacctgaaag aaaacggagt cggcctgaaa atcgtccgcc
    60841 gcagcctgcc cttcggcaaa atcagcggcg aacacggttt gatgtttacc gcatactgcc
    60901 gcacgctgca taatatcgaa gcgcaattat tgagtatgtt cggcgacacg gacggcaaaa
    60961 ccgacctgct gctccgacac ctctccgccg ccgtttcggg cggatactac tacgcgccgt
    61021 ctgtcgaacg cctgcaaaac ctctaagccg gacgatgccg tctgaaaccc tttttcagac
    61081 ggcattttcc cgttttaccg tccaaccgtt ttcatacacg ctatgtccgc acccatccga
    61141 gcattcgacc cgctgaccgc ccccatttcc ggcaccaacc tgattgaggc ctctgccggc
    61201 accggcaaaa cctacggcat tgccgccctg tttacgcgcc tgatcgtatt ggaacaaaaa
    61261 agcgtcgagc gcgtattggt cgttaccttc accaaagccg ccactgccga gctgaaaaca
    61321 cgcctgcgcg cgcgtttgga cgatgtgttg caggttttgg aaagcaaaga aattgccgaa
    61381 cttggagacg acacgctttc agacggcatt gccgcctact gcgccgaaca ccacgaaggc
    61441 gacaccttcc tgcccgcact cttggaacag gctttgcaaa aagaaagccg gacgcgtctg
    61501 attgtccgcc tcaaggccgc catcgggcaa ttcgacaacg ccgccatcta caccatccac
    61561 ggcttctgcc agcgcatcct gcgcgactac gccttcctgt gccaagcacc gttcgatgtc
    61621 gaactgaccg aagaagacgg cgaccgcctg cttgtcccgg ctcaagattt ttggcgggaa
    61681 cgcgtcagcg gcgacccggt gcttgccgca ttggcgttta aacgcaaagc tgtgccgcaa
    61741 accgtccttg cccaaatccg cgcctacctg tcccgcccgt acctgaattt ccgccgtccg
    61801 caggcggatt tgaaacaggc gcagcgcgac gccgaaacct catggcaaac cgtctgccgc
    61861 ctgctgcccg agctggaagc cggcttttgg cgcatccatc ccgacctcaa cggcaacagt
    61921 taccgcaaaa acagcttcgg caaccttttt aaagaactgg cacaaaaatc cgccgccgga
    61981 caactgccct ttctggacaa agacacgcac gaacggctct tgaaactctc atccgacaaa
    62041 ctcgaagccg gactgaaaaa aggcaaaacg cccgatgcgg cagtatttgc cgaattgcag
    62101 aaactggcag acttcgggcg cgatttgaac gcactcgaag aagcggaaga aacaacaatg
    62161 atccggctgc aactggattt aatcgaatat ctcaacagca gccttgccga aatgaaaaaa
    62221 tcgcgccgcg aacgcggttt cgacgacctg ctgctcgatg tccacaccgc gctgaccgac
    62281 aatccgcacg ccgaaacccc cgcccgcgcc gttgccgaaa actgggaaat cgcgctgatc
    62341 gacgagttcc aagacaccga cccgctgcaa tacgaaatct tccaaaagat tttcatcgcc
    62401 cgaaaccgcc cgctgttcct cgtcggcgac cccaaacagg cgatttacag ctttcgcggt
    62461 gcggacattt acgcctacct tcaggcggcg gaagacgcgc ggcaccgcta cacgctcgcc
    62521 accaactacc gcagccacgc cgcgcttatc ggcagcatcg gcgcgctgtt ccgcctcaaa
    62581 gaacgcccgt tcgttttgga aaacatcggc tattcggaag tcggtgcggc gcgtgccgaa
    62641 agcaggttgt cgcccaaacg tcctgccgta cagctccgtt ggctgcacga gaacgacaat
    62701 gaaaaagcca acaaagacgt tttgcgccgc cgtgccgccg actattgcgc cgacgaaatc
    62761 gcccgcgcgc tcaacgaagc ctccggaggc cgtctgaatt tcaaaggccg cccgttgcag
    62821 tcgggcgata ttgccgttct ggtacgcacg cacaacgagg cggtgatgat ttccgccgca
    62881 ctgaaaaaac ggcaggtgca aagcgtcctg ctttcgcgcg aatccgtgtt cgcctcgccc
    62941 gaagccgccg ccctgtccgc gctcatcggc ttctggctcg aaccgcgccg cgccggaacg
    63001 ctgcgctttg tcctgacaag cggcatattc ggttatgacg cgcagcaatt gcacgacttc
    63061 aaccaaaacg aaagcgagat tttgcattgg gcggaatctg cccgaaccgc gctcgacata
    63121 tggcaaaaat acggcatttt cgccgctatg cagcagtttt cccaaacaca cggcatcgaa
    63181 acgcgcctct taagccggaa caacgggcgc agcctgacca attatttcca actgctcgaa
    63241 ctgcttgccg ccgaagacgc gcaaaaccgc aaccccgccg cactgcacaa atggctgcgc
    63301 gatcaaatca gcctggccgg caacaacggc ggcgacaacc gcgccatccg tttggaaagc
    63361 gacgaagatt tggtcaaaat cgttaccatg cacgcctcga aaggtttgca gtatccgctg
    63421 gtgtactgcc cgtttgcgtg ggacgcgcaa gataccggac cgtccgactg gcaaatcctc
    63481 caccaaggcg caaaccgggc cgaacttttg gcaaaggcgc aactgtcgga aagcgaacag
    63541 aagcaatacg ccgacgaaga aatggcggaa cgcctgcgcc tgctttatgt cgcgctgacg
    63601 cgtgccgagg aacagctcaa catctacgcc gcctattctt ccgataccgc cgacaacccc
    63661 cttgcctacc tgatcgaagg cttgccgcaa gacagccgcg aaaccgtccg ccgtacctat
    63721 gcgtgtgaaa aagacggcat aacgatgctc aaacgcaact ggcggcgcgt ggcggacaat
    63781 gccccggccg gcacggattt cgccttcaca gaagatgcgc cgccccctgc cgcctatcgc
    63841 ggcaacgccg atcaagccgc cgaatttgcc gcaaacagca ttcccgaacg cggattccga
    63901 tttgtccgcc acaccagctt taccgcctta agccgccata cccaaacgcc cgacggcggc
    63961 gaagaagatg cctgcccgtc cttggatgcc gccgaaacct cggtgccggc gatgccgtct
    64021 gaaacgccga cggcttcaga cggcatatcg atacacgact ttccgaaagg cacgcaggcg
    64081 gggctgtgcc tgcacgaaat tcttgaagat ttcaaattcg gacaagcagc cgccgaacag
    64141 gaaaccctca ttgccgacaa gctgaaaaaa tacggttttg aagaaatatg gctgcccgcc
    64201 gttgccgaaa tggcggaagc ctgccgcaaa acgccgctga ctggggcata cggcctgtcc
    64261 gacatcccgc ccgggtgccg ccgtcccgaa atgggcttta ccctccacac cgaagacttc
    64321 ggcctcaaac ggctgcgcga ctggcttgcc cgcgacgaca tcaggctgcc cgaagtctgc
    64381 cgtgccgccg ccgagacgct cgacttccac accgtcaacg gctttttaaa cggctttatc
    64441 gatatggtct gccaagaccc cgacggcaat atctgcatca tcgactacaa atccaaccac
    64501 ctatccgcct acacgcggca ggcgatggac gaagccgtcg cgcaccagca ctattacctt
    64561 caggcactga tttacgccgt tgccgccgcg cgctacttca aactgcgcgg acaaccgccc
    64621 gccgccgttt ccgtccgcta cctgtttttg cgcggattgg atggcaaagg cggcggcgtt
    64681 tggcgttggg acatagatgc cgccgctttg gaacagataa aataacttcc cgtcccttaa
    64741 gacgacaacc gcaggagaac cctatgaata tcaaacaatt gattaccgcc gcactcattg
    64801 cctcagccgc ctttgccacg caggcagccc cgcaaaaacc cgtatccgcc gcccaaaccg
    64861 cgcaacattc agccgtttgg atcgatgtcc gttccgaaca ggaatttagc gaaggtcatt
    64921 tgcacaacgc ggtcaacatc cccgtcgacc aaatcgtccg ccgcatatac gaagccgcgc
    64981 ccgacaaaga cacgccggtc aacctctact gccgcagcgg acggcgtgcc gaagccgccc
    65041 ttcaagagct gaaaaaagca ggttatacaa atgttgccaa tcacggcggt tatgaagacc
    65101 tgctcaaaaa agggatgaaa tgacgcaatg ccgtctgaaa ggacaggctt tcagacggca
    65161 tacggcgttc cggcacgcgg cacggcatca ggcgcggcgc gtgccgcccc gtccgaagcc
    65221 cgataaacaa accgataagg aaatacgatg aaacacatac tccccctgat cgccgcatcc
    65281 gcactctgca tttcaaccgc ttcggcacac cctgccgaca aaccgcccac ccaaaacgaa
    65341 accgctatga ccacgcacac cctcacctcg aaatacagtt ttgacgaaac cgtcagccgc
    65401 cttgaaaccg ccataaaaag caaagggatg gacatttttg ccgtcatcga ccatcaggaa
    65461 gcggcacgcc gaaacggcct gaccatgcag ccggcaaaag tcatcgtctt cggcacgccc
    65521 aaggccggta cgccgctgat ggtcaaagac cccgccttcg ccctgcaact gcccctgcgc
    65581 gtcctcgtta ccgaaacgga cggcaaagta cgcaccgcct ataccgatac gcgcgccctc
    65641 atcgtcggca gccgcatcag tttcgacgaa gtggcaaaca ctttggcaaa cgccgaaaaa
    65701 ctgatacaaa aaaccgtagg cgaataagcc cgtcgccgac atatggcgga tggaaatcaa
    65761 atgcacacgc ccctcattcc cccgaagtcg ggaatccaag cggttgggtt accgtgattt
    65821 ccaatcattg ccgaaacgga cgattccgga ttcccgcctt cgcagggatg acgggcgtat
    65881 gcgttttgcc atatcacagg caactttgcc gcaaatctta tctgtcgccg gtatgccttc
    65941 ttgtttcaat ccgccacaaa ccaaatgccg tctgaaacct gatttcagac ggcatttccg
    66001 tttattccct gctgtcgcgg caaatatcga tggcttcctg caaacttgaa acgccgtgga
    66061 ttttcaggtt cggaaactct ttggcattgc gcggcatatt ggctttgggg acgatggcgc
    66121 gtttgaagcc gagcttttcc gcttctttga gccgctcttg tccgcgtgcg acggggcgga
    66181 cttcgccgct taggccgatt tcaccgaaga ccacggtttt ttcaggcata gggcggttgc
    66241 ggaagctgga aagcatcgcg aggatgaccg ccaaatccgc cgccggttcg ccgattttca
    66301 cgccgccgac ggcgtttaaa aaaacatcct gatcgaaaca ggcgatgccg ccgtgccggt
    66361 ttaacacggc aagcagcatc gcaaggcggt tttgttccaa tccgacgctg aggcgtttgg
    66421 gtgtgaagcc gtgcgcgtca tcgaccaatg cctgaatttc gaccaaaagc gggcggctgc
    66481 cttcctgtgt aaccaaaacg cacgaaccgg gcgtgtcgtc gcggtagctg gcgaggaaga
    66541 tggcggacgg gttggatacg cctttcaaac cgttttcggt catcgcgaac acgcccaatt
    66601 cgtttgccgc gccgaaacgg tttttgatgg cgcgtatcat gcggtagttg gaatgttggt
    66661 cgccctcgaa atacagcacg gtatcgacca tatgttccag cacgcgcggg ccggcaatcg
    66721 ctccgtcttt ggtaacgtgt ccgaccagta tcatggcgat acccatttgt ttcgccatac
    66781 gcgtcagttg ggcggcacat tcgcgcacct gcgacacgga accgggggcg gacgtgattt
    66841 ggtcggaata catggtttgg atggaatcga taacgacgac ttcgggctga tgctgtttca
    66901 aggccgcctg aatcgcttcc atgcggattt cggcaagcag gtttacgcct tcggcgtgca
    66961 gttccaaacg ttgcgagcgc agggcgacct gttgggcgga ttcttcgccg gaaacgtaca
    67021 gcactttgcg gctttgcgcc attttggcga tggtttgcaa caacagcgtg gatttgccga
    67081 tgccgggatc gccgccgagc aggatgaccg cgccatcgac caaaccgccg cccaatacgc
    67141 ggtcgagttc gcccataccg gtcggattgc gcggcacttc ggcagcggta acggcggaga
    67201 gggattggac ggtcgaagtg tccgccgccc aagattggaa tcgggcgttt ttcggctcgg
    67261 gcgcggcaaa gctttcctga agcgtgttcc actcgccgca atgcggacat ttgccctgcc
    67321 atttcgggga agtgccgccg cattcggtgc attggtaaag ggttttaagc gtttttgcca
    67381 tgatgttttc cctatgggat ttttgaagtg taaaaaaatc gtctgaaact tcttttctaa
    67441 aaaatttttt attattagtt tgttaatttt gattacaaga ccatatcacg atacaaacaa
    67501 gggtggcttt ctgttatttt gcggcataat gacggaaaat acaaaacagg aatcccaaac
    67561 aatgactttc ccattaaata taatggtaga attgcgttgc gttgcgttgc gttgcgttgc
    67621 gttgcgttgc gttgcgttgc gttgcgttgc gttcagagca tagttcacct ttaaaatcca
    67681 tacaaggatt tttatcaaat ttatgcatag gagtgaaatc ctatgctatc taaacaaatc
    67741 tcaaatctta attcttctag taacaaacca aaaatcctat ctctattttc aggatgtggc
    67801 ggtttggatt tgggctttca tcaagctggt tgtgaaactg tttgggcgaa cgatttctcc
    67861 cattgggctt gcgaaagttt ccgtaaaaat atcggcgatg tcatcgtaga aggtgatatt
    67921 gaacaaatta atccgaatga tccaactatt cccgattgcg acatcatttt aggcggattc
    67981 ccttgtcaag atttttccat gatttggaaa cagccgggct tagagggtga gcgcggcaat
    68041 ctttataaaa gctttttacg ttttgtaaat gcaaaaaaac cgaaagtttt tgttgctgag
    68101 aatgtgaaag gtttattgac tgccaacaag aaaaaagcca tccagcaaat tattaccgac
    68161 tttgaaaatt gcggttatta cgttcaggcg aagctgtata actttgcaga atttggcgta
    68221 cctcaatttc gtgaacgtgt gctgattgtc ggagtacgtt tggatacagg atttgatttt
    68281 cgccatccgg aaccgacgca caatgaaact ggcgaaaacg gcttaaaacc atatgtaaca
    68341 gcaggtcagg ccatatccaa tattccacaa aatgccagta ataatgaatt actgaaaatc
    68401 agcgataaaa cacgccgtat gttggaatta attcctgaag gtggaaattt taccgatatt
    68461 cctaaagatc atcctttata tgtgaaaggt atgattagcc acgtttatcg tcgtatgcat
    68521 cggaacgagc catcaaaaac aattattgca gcaggtggcg gtggtacttg gggctatcac
    68581 ttccctgaac cgcgtgcttt tactaataga gaacgagcaa ggcttcaaag ttttcctgat
    68641 gattttgagt ttgtcggatc aacaactgaa gtacgtcgcc agattggtaa tgctgttcct
    68701 cctcagggcg tggttgaact ggcaaaaagc attttaccga ttttttcaga caactatgag
    68761 aaagtagatt tgcatgagaa attagtcgaa gaaaaagaaa ttttattcca tgaccgacta
    68821 agcaaaattc gagggggaaa acaatgaata cagttttttc caatatcgcc aatgccaaaa
    68881 tcactgaaaa atctcttaat gcggtttgga tggatttatt taaatcagcg gatgaagtgt
    68941 tgatggcgac aggttatgta tctaatgacg cagtggttga gttacataaa attttggagc
    69001 taaacgatca tattcaaaaa atagatttat tggtcggaat gcattatttg gaaggtttta
    69061 gccatttaca atatgacagc ttatgtaaac taaatgattt tttgcgacat gagaaaagag
    69121 gagccgtcta tgtttccccc tttgtgaaat ttcacggcaa aatgtattcc tttaaaaatt
    69181 atcaaaaaat caatggatta attggttctg ctaacttaac gtgtttttgg gatagcacgg
    69241 aacgtactta tgaaactatg ttgcatttga acggtaagcc tgcacagatt ttgcaggcag
    69301 atattcaaag tactattcat aagctaggca aaaatatcca agaagttgaa agaccaagta
    69361 aatttattga acataatagc catttagaaa attgtttggg tgttcagaaa attgcaccag
    69421 aacagattcg gcaattattt gcccaaacgt cagaatatca tttctcaatt ccagctaaaa
    69481 ccgaagaaaa aagtaatttg aatgtatttt ttggagaagg tcgacgagat aaacgagggt
    69541 ttgtcaaacc acgaccttgg tatgaagtag agttgattgt atccaaagac atcacttcac
    69601 aagaagggta tccggttctg aagtctttta cagttattac cgatgatggt tggcaatttc
    69661 aatgcaagac atcgggggat tactccaaaa acttccgctc agaaaatgat ttaaagacac
    69721 ttggtaaatg gattaaaggt cgtttggaaa gtcatggctg cctgcaaaat aacgagaaga
    69781 ttacgcatga aaccctacgc gaatacggta acgatcattt tgagttacgt tcaaccgata
    69841 atcctgatgt ttggctgctc tcatttaagg ggaaaaacta aatgttgcga acctacttga
    69901 accaattgac ccctcccgaa ttagctgatt ctgttaaaaa taccgttgat ggttttatgg
    69961 aaaaattgtc acaaaccgag ccgaaaatcg cccaaaatgt tttattgctg ggcaatgttc
    70021 aaagtggcaa aacggcacag gttttgggtg tattaagtgc gctggctgat gatggcgacc
    70081 acaaagtttt tttgtatctt actactgaca gcgtagattt acaggatcaa acagtcaaac
    70141 gagccaaagc caatctgaaa aactttatcg tattgtctga agctgacgac cgaagtttta
    70201 tggaagtaat gaaggcagag aaccccatct tggtcgtgat taaaaagaat gcccgtgtat
    70261 tgaaacgttg gcgtaatttg tttgccagtc aaagtagcct gaaaggctat cctttggtta
    70321 ttgtggacga tgaagccgat gctgccagcc taaatactaa ttctgataag ccggctaaag
    70381 atgccagtac tatcaataaa ctgctaaacg acatcaaaaa ttcctgttgt caaagcctgt
    70441 ttatccagtt gactgccacg cctcaatcgc ttttattaca acatgaagaa tctgattggc
    70501 aacctgaatt tatccacttc tttgaagcag gcgaaaaata catcggcggc aactttgtct
    70561 tttctgatcc accaagctat atagttcgtt ttattgatag cgaattggat gatatgaaag
    70621 acgaaagtgg tgaaattgcc gaaggagcaa aacaggcatt gcttagtttt ctaataacct
    70681 gtgcagaatt cgcgctatgt gataaggcta actgtaattt tgcactacat ccaagctata
    70741 aaattcaaga tcatcaagcc ttttcaaaga agatccaagc ctttttaaat gacttagtgc
    70801 aagcagtcaa taatggggaa gaccttgcag gtagctttaa agaaagttat ttggatttgc
    70861 aaaagaccaa gcccgatatt catcactttg atgaaatata cgaaaaattg acagcacttt
    70921 tagagaataa acaaatctct actcttgtcg ttaactcgca gacagaaaca gattttgact
    70981 tagaaaaagg tttcaacatc attattggtg gtaatgtgat tggtcgtggc ttgactattc
    71041 cgaaactaca aacggtttat tatagtcgca ctgccaaaaa accgaatgca gatactttct
    71101 ggcaacattc gcgtattttt ggatatgacc gcgataaatc attattacgg ctttatattc
    71161 cgttcgatgt ctattacttc tttgtacaac tcaatcaagc aaataatctg attattgggc
    71221 aggcaaaaaa ttcaggcggc aatattcagg ttatttatcc gaaaaatatc aatccaactc
    71281 gaaaaaatgt attgaaattc gatagtatca atcaaattgt gggtggtgtg aattattttc
    71341 cactccaccc aaacgaagat aatttatcgg agattaacaa aattttgcct tctattttga
    71401 aagatgaaat tcaatctgat ttatatcaaa tagatataga agatttattc ttggttctag
    71461 ataaattggg tcgttatgtt ccagatgact ggaataagga aaaatttatt gctggtgtag
    71521 aagcattaaa agcacaacgt ccaagtttta aaacctatgt tttgatcaaa accggacgta
    71581 aactttctcg agcaaccggc acaatgcttt cggaagacga ccgtaaatta ggagaaaaat
    71641 atcccaatga tttattcctt acgctttatc aagtagtagg aaataaagac aaaggttggc
    71701 agggaaaaga tttttggtta cccaatatca agctaccaca caatggcttg gtgtaccaaa
    71761 gtgcaaaatg aagcatatgc cgtctgaaaa cttccgattc ttcagacggc atatcgggcg
    71821 gtttcctgcc ggcttacccg ccgtgatact gccgcgacag ctcgtgtacg gtgtcgacta
    71881 agattttggc gtgttcgggg tcggcgtgtt ggttgatgcc gtgtccgagg ttgaagacat
    71941 ggccgctgcc gtgtccgtag ccggtcagga tacgtgcgac ctcggtgcgg atggattcgg
    72001 gcgtaccgaa gagggcggac gggtcgaagt tgccttgcag ggcgacttgg ttgccgacgc
    72061 ggcggcgtgc ttcgccgatg ttgcacgtcc agtccaagcc caatgcgtct gcgccgattt
    72121 gggccatgct ttccagccac agcccgccgc ctttggcaaa cacgataaca ggcacgcggc
    72181 gaccttcgct ttcacgtttg agtccggcaa cgatttggcg gatgtatttg aggctgaact
    72241 ctttaaacgc cgcgtcgctc aacacgccgc cccaagtgtc gaaaatctgc accgcctgcg
    72301 cgcccgcgtc gatttgggcg ttgaggtagg cggtaacggc ttgggcgttg gtatcgagga
    72361 ttttgtacag caaatcgggg cgcgagtaca tcatggtttt gatggtgcgg aattctttgc
    72421 tgccgccgcc ttcgaccata taacaggcga gcgtgaacgg gctgccggag aagccgataa
    72481 gcggtacgcg gccgtccaat gctttacgga tggaagttac cgcgtcgaaa acgtattgca
    72541 gtttttccat gtcgggaacg tgcagcttgg cgatgtcgga ttcgtgttgc aaggcgcgtt
    72601 tgaatttcgg gccttcgcct tcggcaaaat acagccccaa gcccattgca tcgggaacgg
    72661 tcaagatgtc ggaaaacaaa atcgccgcgt ccaaatcgaa gcgttccaaa ggctggatgg
    72721 tgacttcggt cgccaatccg gtgtttttgc acaaatcgag gaagctgccc gctttcgcgc
    72781 gtgtggcttt gtattcgggc agataacgcc ccgcctggcg catcatccaa atcggtgtgt
    72841 attcgacggg ctgtttgagc agggcgcgga ggaaggtatc gttttttaaa agagtcatac
    72901 gggttttctg tcagtctgtt tgtcaagtga aatggttttc agacggcatt tcaacgatgc
    72961 cgtctgaagg tttcaacggg tttcggcgga agggcggttt tccccggcaa cgcttgccaa
    73021 aaccaagttg cgctgtgctt cggctttttg cgactgtgcg gtttcgtcaa aaacctttgc
    73081 caacaccaaa cgcgccggaa tactcggctt cagtgcaata ctcgcttcaa ggtagccttt
    73141 tgccttaccc caaagtttgc ggccgtaggc gagccggccg agatacatca gcagaagcgc
    73201 gttatcgggc tgttctttca gccaagaatc ggcaaaatcg atggctttct gctgttcgcg
    73261 ctcgcccaaa aagcgcacgc tttcgacaaa ggcttccaaa agctcggggc ggcggttgtg
    73321 cggataatgc tgtttgaccc atttgaccgc atcggcatac agtcccaaac gttcgtactt
    73381 ttccgcaacc gatacgctca attccccgtt tttgaggctg tcgggaatcc gcttcaggca
    73441 ggttttcaaa gcggcggcat cggcagcatc cgccatctgg cggcggtatg cccaattttg
    73501 ataccgttcc atttccgatt tgcccaacgc gcccgccttg gaaagttttt cggtttttgc
    73561 cagaacctgc aacgcatcgc cccgatcgaa ggcgtaacga agttgcagac gcacgaggcg
    73621 cgtaaggttg gcattcatct tcgccgccgc atgaagattg gcttccgccg cttcgtaatc
    73681 gcgccggttc agtgccgatt ccgccagcag aagatagcgg gaaagctgct gtttttccgg
    73741 cagtttggcg atttccgcaa gataacggtc gcgcagctcg atattttcca tctgtcctgc
    73801 cgcgtgcgcg cccagcatca atgccaaagt ccgattgtct ccggcctctt tgttgcccaa
    73861 cactcgagag gcttcgagtt ccgccttttc aaaacgccct tcgaaatacg ccaaacctgc
    73921 cttattcaag gcaagcgcgg ccttgcggcc tttccgcgcc gaaccggaac gctgcatctt
    73981 ttcggggata ttaagtacgc cgatgatgaa tttaaacagg aaataccaca ccacgacggc
    74041 aatcagcgaa cctaacacaa aagcgtgcag gttgattctg agcatggtct gtccgagtac
    74101 gatatacacg tcgccggtgt aaatgcccga agccagcgcc agtccgacgg cggcggcaaa
    74161 caggacgaca atccagacta ccgttttcat gcgcgttctc ctttagcgga cggcgaacgc
    74221 cctgcctttt ccggtgcggg ctgttttttt gcaggctgtt ccggtttgcg ttccgaaggc
    74281 agggacggtg cttctatggc cttcacatcc gatgcggcag ccggttcgga agctgtgttt
    74341 tcgtttgccg gttcggaagc cgcctgttcc gcttcttgat tttccgccgc cgtcatgcgc
    74401 gtaccgtcgc gataggcgcg gacggcattt aaactggtct tcaagccgtc atccgccgtc
    74461 atgcgtacat ccagtgtttt caattccgcc agttctttca gccacgactg cgtggcgggc
    74521 gacttggcat cgaaatactg tctgacggcg gcttcggcat tgttcaaatc gccctgatag
    74581 acttcgccgt tgcgctgcat caatgcggtg cgcgcatcca acaggcgcag acgcaggttt
    74641 tcacgcacaa aatatgcctg ttcgggagaa atcagcatgg catcgttgtt ttccaaacgg
    74701 cggatttcga ccagcccctt caatgtgccg agggattttt cccatacgtt ctgccaccat
    74761 gaagcggaaa cggcttcgtt gcgggcctgt acgcccggtt tcaatacgcc gtccaaaatc
    74821 agcggcagtc cggatacggc ggtttccaac cggtcgaggc gcaatgccgt gccggaaata
    74881 tcgacatagg gacggttttt cagttccgcc aaatcgctgc tgaccgcctg tttgatcggc
    74941 agaagctctg cctgatcgaa acgggacagg cggctgtcga tatgctccaa cacgccgact
    75001 gccgtttgga tattgccggt caacaccagc tgttgcgccg ccagattgag tatggtctcg
    75061 gtttcgtcca ccagccaatc ggcacgccct ttggtcagct cgcgataggc tttttgcgtc
    75121 atcaggattt gttcgccgtt ggctttgaca ccgctgtcca aacggtcgag ttcggactgt
    75181 atggcacttt ggcggttgag gttgtctttg agcagggcgg cgttttccga ctcgcccaag
    75241 gcggctttgt cgattttctg gttgaatgcc agctcttggt ttttcaagac attctgtcct
    75301 tggacaaaca aaaaaccact tgcacccaaa cccaatgccg ccaataccaa ggcgcaggcc
    75361 gccaaagcgt tgctgccgga ctgtttgacg ataaaaggag cggcgggaat gtgtacttct
    75421 gtttcgtttt ctttgcgtgg ggaagaggtt tcagacggca tttcttttga tgcctgtatc
    75481 tcgcgtacgg gttcggatga tttgttttca ggttcgccca ccgttttgct ccttattggt
    75541 ttgaggttcc gggaaagacc atgccgtctg aaacggaaat ggaagaatag gaaagcgcgg
    75601 cttccagcgt agggacggtt tcgaccgaac acacgccttc gcgcttcaat gcctccgcaa
    75661 tgcgcggatg atgggtaaag tatagcaagg atttgaagaa tcgggaaaat tgcggcggaa
    75721 gctgtgcaaa cagcgactgc acaagttctg tggacgtaat ataggcggcg gcaatatttt
    75781 cggtttggaa attttgaaag ttcaaaggtt tatgccgtct gaaatagact tctgccacct
    75841 ccgtccgaaa acctttctcc tgcaaggcat tcatcagaaa atcccgcccg ccgtgtccgc
    75901 gcacagacaa tacgcgcgca ccttcgggca gactgttcca aaccggcagg cgcaaaaccg
    75961 cctcgctgtc gttgccgtct tcaggtgcaa acacgcccgc gcccgcataa cgcgccaagg
    76021 cacggcggct gccttgccct acggcaatgt gcgcctttat gccgtctgaa aggttaaggt
    76081 acgggacggc ggtttcaacg gcggccggac tgacccaaaa cacggcatcc gcacgggcat
    76141 acatttccgg cagccgtttc aggcttgctt cgtcggtttc gatttcaatc ggactcaaca
    76201 cttccgcctg ccatccggca cgacggcaga cttccacatc gtccttcgcc ctgcccgacg
    76261 ggcggacaat cagcatgacg ggcttcgctg tttccatcgc cttatccgcc ccgattaccg
    76321 ggaaaaatcg tcatcgttga aattcgcctg cgcggttttc ggcgtacagc ctacccgtgc
    76381 gacttccttg cccctacgct ccacaacgac accgctgctt tccgtatgag tcacggaatc
    76441 gaagcctgtc catacggtgt acataaattc gccgttttgg aatttcatcg ttgcccggcg
    76501 tccgctgccc atgccttgcc acctgtcgga acgccccaac aggtcagctt tgctgttgcg
    76561 tatggcaatt tctttttttg ccgcactgcc gaacgaatat tcgtaaagat tgcggttgat
    76621 tttttgaact tctatgtatt tgttgttgtc cgttttacag gaaaataccg tatccgccca
    76681 cacggtttgg gcggcagccg acagaagcaa tgcggcagaa aggaacattt ttttcatcat
    76741 aatttcctta atggttgaaa ccccgccatt cggacatccg tccttcgtgg cagcagcatc
    76801 agattttatt tgaaaggggt gtaacccctt tcaaattagg gcaacacata gggcggcgct
    76861 ttatgtgtcg tcctgtgtgt tgaaacatca ttgcccgtat cctgaaatag aaggctgtgt
    76921 tttaacaatc cgttgattgc ttaagatgaa ttcacactaa aaaacagttc tctatttcat
    76981 ctattacggc gggttgtttt gtttataagg gtggtaatct acaaattgtt actaaatagc
    77041 aaggtgtgaa tgaaacggca gatataatca aaatcttctt gttggtcaaa atacggtaac
    77101 ataatgcttt ttaatttttc gcctttgggt ttacgtccat aagaaaacag atagcggttt
    77161 gctgtaatac atttagcgta atacagtttt acttctattg gcatatcgga ctgtatgggt
    77221 gttaatacgg cgacatcact atttgctaca aattcacaag ttgaaacata gctataagta
    77281 tggctacctt cgccattggt agaagtgaaa agcgtatggc atggaaaaat atgtttttca
    77341 tcaacgctat cacgagcgat ataacttcgt agcgttcttg cttgcgtact ggcaggacgg
    77401 atatagacaa ccgtgtcttt ttgccgttgt tcaaattctt ttaatttggt tgcggcaatg
    77461 ccgttttgaa tagtaaaaat ttcatcaagt cgttttgatt gtcgaaccac aggcaaatca
    77521 agcggttctt ttaatttcgg ttcggttaca cccgcagatg gatttaaatt cacgctttca
    77581 acccagcttg ggatctcatc caaagatggc acaaacagtt gtcgtaacgt gcggttagct
    77641 tccctaccgc aagcagaaaa acggtaggca ttggcaaaaa tagcagcaca ataaaagagt
    77701 ttttgttgtt ctgtcatttc tgttttggga tttaaaacag caacattttg agctgtataa
    77761 aatggtttgt tttgtaaaaa agtagataaa acagaaccac ctaatgcaac agttaataat
    77821 cctgctggat aaggttttgt atttttcaat aaatccacag atgctgttac accattattt
    77881 ttgcctgacc tgcctacaaa gttgattgtt ggattgaagc tgctcatttt attcaaatct
    77941 aattttgaac cgtaactcac atcaaaaatc tcttgcaatt caaccattat tcatcgtctc
    78001 cgaagtcgtt attgtcatcg tcttcatagc tttcattatc caaattttca gtcggcccta
    78061 cttccgctcc cagtagttga aataaagcaa attcacgcac gacttgctca aaatctgctc
    78121 gggtaatttt ggaataatca gtttccaaat aggcttcggc aacccattca tcgttatcag
    78181 ttacaaatgc tgttaccgat tctcctgtgt gaacttcgtt gtttaaataa tgttccaacc
    78241 agcgcgcttt aatgccctgc cagcggtcgt ataaatcaat gcgtcccaag tgtttggttt
    78301 tttcaaaacc gtcatcacgc cagtagccaa accacgtttt gcgacaggct tttttcgtgc
    78361 tgatttcttc ttgtgtgtcc gggtcaatca cgactgtctg aaaatgcggt ttatgggctt
    78421 caaatacgac aatgcacgtt accgtgccaa ctgggtaaaa cagttcgctc ggcatagaca
    78481 tcacggcttt taagcgatga tatttcacaa tttcgctttt ggctttgctt ggtgcaatca
    78541 cgcaactgac tggaataatg gcaatgcctg tgccaccttc tgccaacata tccaacattt
    78601 ctttgacaaa ataaagttca tgcaattcgg catcgctttt ggattgggcg taaggtggat
    78661 tgagaaagcc aacatttggg cgttttaagc ctgtttcagg tttgggattt ttgattaaat
    78721 cctgaaaatc agtcatgaaa caactgcttt ggtgtaaatt ggctttaccg tctccgcgta
    78781 aaatcatatt actggcggca agagcaaaca tttttgggtt gttttcaatg ccaatcagtt
    78841 gatatttttt agagatggct tacccaaatt gctaattcta gcaacagatt taccgtctaa
    78901 attgatacca caaggaacac aaacttgttc tttggaaatg ccacgtttcg ttgctctcgt
    78961 tcctcgtttg tgagcaggac gtggcagatt aaaatttttg tggtgccctt tgtaagaaat
    79021 cgtcgaataa gtttcatcag cttgcacaat gccgtctaac tcaacttcat tcatcatgtt
    79081 ttgaagtgcg tccaaaattt tgtgccgcca agtaaacgca gtagcaagat tgattttgca
    79141 aatttcagca catttgcgaa gtgggtattt ttcaatcata caatgaatgt atttttccca
    79201 aacttcaatg tctttttgcg tgttgtagag aatagtgcct gtctgctcaa caaaagactt
    79261 tttacaatca cgacacaaaa aacgttggtt gccacaatct ttgccatttt tgacgaaatg
    79321 tgtagattgg caatgtgggc acttcgtaac ttttcttggt tcaatgactt ttttaacctg
    79381 ttcttttgag ctgacagaag tcaaaaaagc ctgtttatcc gtatcggaca aattttcaaa
    79441 aagttgtttt aaaatgtcta attcttttgt tacgcccgac attataaaat cccttatagt
    79501 tcaatttgtt ataagggatt ttatcaaaaa ttagtgcaaa aatcaacgga ttgttaaaac
    79561 acagccaaat agaaaagaaa atgcccgttt tacggtcttc tttccgacaa aatgccgtct
    79621 gaaacgaatt tcagacggca tggtcgaaaa ttattgcgcg gcaagcggtt tgagcaggag
    79681 ggtttcgccg ctttgcacga cattttccag caaatcgatg ttcggatgct tccccggatg
    79741 ggcgcgcgcg tcggcaacat gttcggcaaa ccatatcaaa ccctgttctg ccgcttcttt
    79801 acccaatttt ccatcaaaac gttccgccaa ggcattgtac agcttgagcg aaccgagctt
    79861 gccctgaacg gcgggaatgt ggtgaaccgt tttgccttcg gcatcctgaa catccaaacc
    79921 gcccagatgg tcgattgaag gaaatgtggc gagatagtct tggaaattca tatcaaatcc
    79981 gaaaatcagt cagttagcgc gtgccgaaaa tcttgtcgcc cgcatcgccc agaccgggaa
    80041 taatatagcc gttttcgttc aaacggctat ccagcgcggc ggtataaatc gtaacatcgg
    80101 gatgcgcatc attaacagcc ttcacgccct caggggcggc aaccaagact aaggctttga
    80161 tgttgcggca acctttttct ttcaacaggt cgatggtggc caccatcgaa ccgcctgtcg
    80221 ccagcatagg atcgataatc aaagccggac gctcgtccat actgtccaca aatttctcaa
    80281 aataggaaat aggcttcagc gtttcctcgt cgcgctgcaa accgaccacg ctgattttgg
    80341 cagtcggaat caggtcgagc acgccgtcga gcatacccaa accggcgcgc aggatgggga
    80401 cgacggtcag ggttttgcct ttgatgcggt cgccttcaat ctgtccgcac catccgtcga
    80461 taaggtattt ttcaatttca aaatcacggc ttgcctcgta tgccatcagg cgcgccaact
    80521 cggtggtcag cgtacggaat ttgtaggtgc tgcaatccgc ctccctcatc agggtcagtt
    80581 tgtggcggac gagcggatgg ttgataacgt taacgttcat cgcggtgttc cagtgtgatt
    80641 ttgagacgcg tcattatact tttttttaat ttgccggaaa agaaaatgcc gtctgaaaca
    80701 cgttcagacg gcattgccga atccatcagg ctttcagcag ctcttgcagc tcgcctgcct
    80761 catacatttc catcaggatg tccgaaccgc cgacaaactc gccgttcaca taaagttggg
    80821 ggatggtcgg ccagtcgctg tattccttaa tgccttggcg tacttcggga ttttccaata
    80881 cgttgacagc aacgtaatcg gtgcagcctg ccgcgttcag gatttgcacg gcgcgggaag
    80941 agaaaccgca ttgcggaaac tgcttcgtac ctttcataaa caatacgacg cggtgtgtcg
    81001 ttactacttc tttaatttgg tcgtggatgg aagccatcgc ttattccttt tgataaagtt
    81061 gacaaaattt gtcaggtagg gcgcattata cgcaaaagcg gaaacttccc acaatatttg
    81121 ccgtatatcg cgcagtatca atgttgcgaa cggcaatatt cccactcctc gacaatcctg
    81181 ctgtccggca aatggcacag ggcatatccg ccgccgcttt cgtctttttt tacttcccgt
    81241 ctgccgcctt tggcaatgca gaatttggaa gcggaatttg ccatgcctgt ccgaaacgtg
    81301 ttggcaggca gccggcagca gcgcggacat gcatacggat gaattgcctt catggttttc
    81361 cttatcgtcg gcacagggtc agacgacccg catatcgggt acttttttaa acagccggta
    81421 aaagttttcc gtcgtatatg ccgcaacctg ttccaatgtt tgattccgca atttggcaat
    81481 atgttcggca gtatgacgca caaaagccgg ttcgttctgc ctgcctcgtt tgggaacggg
    81541 ggcaaggaac ggcgcatcgg tttccactaa aatgcggtcg tccggaacgt atttcgccgc
    81601 ctcctgaacc aagggggcgt ttttaaaggt aacgattccc gagaaagaaa tataaagccc
    81661 caaatccatg gcagcccggg caaaaccgac atcctcggaa aaacagtgaa tcacacccga
    81721 attggtttta cattctttta aaatcgccaa ggtgtccgcc gccgcatcgc gcgtatggac
    81781 gataacgggc agtccggttt gattggctgc ttcgatgtgg tctgcaaagc gtttgtgctg
    81841 ccaagacaaa tcgcctttgc accagtaata atccaaaccc gtctcgccga tgccgaccac
    81901 tttcggatgg gcggcggctt cggccatttc cgcaatggaa aattcttcgg cttccttgct
    81961 gtcgggatgt acgcctatgg tgcaatagat gtgttcgtgc gcttcggcga cggcaaagac
    82021 ttcggagaag ctttccctac tgacgctgat ggcgagtgcc tgccccacgc cgtttgcttc
    82081 catgttggac aaaacttcgg gcaggcgttc tttcaaacct tcaaaattga ggtggcagtg
    82141 cgaatcgatg atatgcatag cgttacatgg taaacgtagg tctgctgccg ctgattgtgc
    82201 tgcccagttc gacttcgatc tggtctttga ctttcaggca gttttcgtgt tttgtgaatg
    82261 ccagccccac ccctttgggt ttggaggaag tactcgcagg attaatccag gcgaccttgg
    82321 tcggcaggaa cagtttgggg aagttgagga tttctacggc aagcagaata tcgtccccga
    82381 tggaaaatac gtcgtcggtc tgcacaaaca gaccgccgtg ttccaaaaac ggcatatagg
    82441 agctgtacag cagattcatg tctttcagct gcaacgacat catttttgcc ggaatatttt
    82501 gtccgtctga cataattcac ctgttctctt tcttcaattc caaatagttg atgagcagat
    82561 gctcgatctg cattttgaca tttaaagtat gaaacccgta aggggcaagc tgtttgagca
    82621 tatcctccgc cgcaaataca ttgcgcggac ggaaacccga tgccgtctga agcagcctgt
    82681 cttcataagc gggataatag acgggtttca tgtgttgcag gcacaagcct aaatcgacca
    82741 gccatttctg catccacccg acaaatacgg cgagcggaag tttttcctta tcgaaaagcg
    82801 cggcgtaatc caaaatcttc aacaatctcg gctctgccaa tatctccagc agctttgccc
    82861 tcaattcccg aagctcgcct tcctcctgaa acagcggcgc acccgaatgg aaagccagac
    82921 gttcctcagg ctccgccaca cccctgtcgc gcagatacgc caatgcttcc ccatgcgaag
    82981 gagcgggcaa aaccttcttc cggcagcggc ttttaatggt cggtaaaacc ttgtccgccg
    83041 cgtggcttac cagcaaaaag accacttgtg gcggcggctc ttccaacact ttcaacaaac
    83101 tgttggcggc ttggacattc atactttccg caggatgaat caggatcacg cgcaaaccgc
    83161 cccgtaccga agtcaggtac acattatcga tgatttccct gacggcatcg attttgatcc
    83221 gcaacagttt gcgtccgttt tcgggttcgt ccgccagagg cgtgatttcg taaaaatcgg
    83281 gatggcttcc ccgtccaaac agatggcagg acatacattc gccacagggt ttgcagcccg
    83341 gtgcaggggt ttcgcacaac agcgccttcg ccgcaaagcg ggcaaacgca gtcttccccg
    83401 tccccttttt gccaacaaac agccacgcat taggacggct cgtccaatgc tcggcaatct
    83461 gccgccattg ttcctgatgc cacggataaa tcatgtcaaa ctgcccgtcc ggaaatccat
    83521 accgccatca gagcagttcc aaatcggggt cggaactttg tgccctgcgg ctgcgcaact
    83581 gtaccgccaa acgcaaatca tgtgcggaat cggcattttt caaggcatcc tgcaaggaaa
    83641 tctcgccttt ttcatacaat tggtaaaggt gttggtcgaa ggtctgcata cccagggtag
    83701 tggatttttt catcacttct ttgatttcat ggatgttgcc gttgtgaatc aactccgaaa
    83761 tcaggggcga attgagcagc acctcgactg ccgccaccct gcccttgccg ccgtctcgcg
    83821 gaacgaggcg ttgcgaaata aacgcctgaa ggttgagcga caaatccgtc agcaattgtt
    83881 cgcgccgctc ctcggggaag aagttgatga tgcggtcgag cgcctgattg gtgctgttgg
    83941 cgtgcagcgt cgccatacac aaatgccccg tttcggcaaa ggcgatggcg tagtccattg
    84001 tttcacggtc gcggatttcg ccgataagga tcacatccgg cgcctgacgc agcgtatttt
    84061 tcaacgccgc catccagttt tccgtgtcca cgccgacctc gcgctgggta atgatgcagt
    84121 ttttgtgttc gtggacaaac tcgatcggat cttcgatggt gatgatgtgt ccgaacgaat
    84181 tttcattgcg gtagtcgata agcgaggcga gcgaagtcga tttgcccgag ccggtgccgc
    84241 cgacaaaaat aaccagcccg cgttttttca gcgcaacatc cttcaaggcc ggcggcaggt
    84301 tcaggctttc aaacttggga atcttgctgg taatcgcgcg gaataccaac gccgtcgcac
    84361 cgcgctgtat catcgcattg acgcggaagc ggctggtgtc cggcaggctg atggcgaagt
    84421 tgcactcgtt ggtcgatgaa aattcttccg cctgcttcgc actcataatc gaaaaggcga
    84481 tttccataca tttttccgcc gtcagcggtt cgtccgtgat gcgggtgatt ttgccgtcca
    84541 gcttcatagc gggcgggaaa tgggtcgtca caaacaggtc ggaacctttg tttttgttca
    84601 tatggcgcag ccatgcgaac agttcttcct ttgcagtcat attttcatcg gtaaacatcc
    84661 tcgtccccca atcttcaaac aatctgtacc ttcccattct accaaaaatc cgccgtgctg
    84721 tctgaaaccg tttcagacgg cacggacaaa ccgcctccac tcaatcctgc cgtgttagcg
    84781 tgtataattc ggcgttctat ccgaaattca cagcaggtta gcaatatgtc tttgaaaaca
    84841 gtagccgtta tcggcgcgat ggaacaggaa atcgagcttt tgcgcgagat gatggaaaat
    84901 gtcaaagccg tctctttcgg cagattttcc gcctatgaag gcgaattggc gggaaaacgc
    84961 atggtgcttg cattgagcgg catcggcaag gtcaacgcgg cggttgcaac ggcttggctt
    85021 atccgtcaat tcgcaccgga ctgcgtcatc aacaccggca gcgcaggcgg tttgggcaag
    85081 ggtttgaaag tcggcgacgt ggtgattggc acggaaaccg agcaccacga tgtcgatgta
    85141 accgcattcg gctatgcccg ggggcaagtg ccgcaactgc cggcaaggtt cgcttcagac
    85201 ggcatattga ttgaaacggc aaaacgggcg gcgcggacgt ttgaaggcgc ggaggtagaa
    85261 caaggcctga ttgtcagcgg cgaccgcttt gtccacagca gcgaaggcgt ggcggaaatc
    85321 cgcaagcact tccccgaagt caaagcagtg gaaatggaag cggcggcgat tgcccaaacc
    85381 tgtcatcagt tggaaacgcc gttcgtcatc atccgcgctg tttccgattc ggcggacgaa
    85441 aaggcagaca tcagctttga agagtttttg aaaacggcgg cggcaagttc tgcaaaaatg
    85501 gtggcagaaa tcgtcaaatc tttataaaac ctttatcaaa tcatgccaaa acagcggttt
    85561 tccgttagaa tatcgggcta tttccacgtt gcgcccgata ttcggacgta ccgctgtttg
    85621 ttcagacggc atttctaaaa cctaccgttc agcattaaaa acaactcaat gaaaaatatc
    85681 cgaaatttct ccatcatcgc ccacatcgac cacggcaaat cgacgcttgc cgaccgcttc
    85741 atccaatatt gcggcggttt ggatttgcgc gaaatgagta cgcaggtgct cgattccatg
    85801 gacatcgaaa aagagcgcgg catcaccatc aaagcgcaaa ccgccgcact caactacaaa
    85861 gcacgcgacg ggcaggtgta tcagcttaat ttgattgaca cgccgggaca cgtcgacttc
    85921 tcttacgaag tttcccgttc gctgtcggct tgcgaaggcg cgcttttggt cgttgacgcg
    85981 tcgcagggcg tggaagcgca aaccgtggcg aactgctata ccgcgattga tttgggcgtg
    86041 gaagtcgtgc ccgttttgaa caaaatcgac ctgcccgccg ccgaccccga gcgtgtggaa
    86101 caggaaatcg aagacatcat cggcatcgat gccgtcggcg cggtgcagtg ttcagccaaa
    86161 agcggcatcg gcgtggaaga cgttttggaa gaaatcgttg ccaaaattcc tgcgccgacc
    86221 ggcgacgaaa acgcgccgct gcaagccgtt atcgtcgatt cgtggtttga caattacgtc
    86281 ggcgtggtca tgctgattcg agtgaaaaac ggcaccatca aactgaaaga caaagtgcgc
    86341 tttatgagca ccaaggcgga aacgcaggtc gagcagctgg gcgtattcac accgaaatcg
    86401 gttcaaaaac aagaactaaa agccggcgaa gtgggttttt tgattaccgg cgtgaaagaa
    86461 ttgggacagg cgaaagtcgg cgatacggtt actttggttg ccaaccctgc cactgagccg
    86521 ctgcccggtt tccaagaagt acaaagccaa gtatttgcgg gtctctatcc tgtggaaagc
    86581 cacgattacg aagccttgcg cgacgctttg gaaaaattgc agctgaacga tgcttcgttg
    86641 aaattcgagc ctgaagtttc tcaagcattg ggcttcggct tccgctgcgg cttcttaggc
    86701 ctgctgcatt tagaaatcgt gcaggaacgc ttggaacgcg agttcgacat ggatttgatt
    86761 accaccgcgc cgacggtggt ttacgaagtc gtgttgaaaa gcggcgaaaa aatcgaagtt
    86821 gaaaacccgt ccaaactgcc cgacatcggc agcatcgaaa ccattctcga gccgattatt
    86881 accgcgacca tccttgtgcc gcaggaatac gtcggcaacg tcatgacttt gtgtaaccaa
    86941 aagcgcggcg tgcaggtcaa tatgcagtat atgggccgcc aagtgatgct gacttacgac
    87001 ttgccgatga acgaagtcgt aatggacttt ttcgacaaac tcaaatccac ttcgcgcggt
    87061 tatgcttcgt tggactacca tttcaaagaa ttccaaccgt ctgatttgat taagctcgac
    87121 attatggtta acggcgaaaa agtcgatgcc ttaagcctga ttgtacaccg tcaaagcgcg
    87181 gttcacaaag gccgcgagct ggcatcgaaa atgcgcgaac tgattccgcg ccaaatgttc
    87241 gacatcgccg tccaagccgc catcggcagc cggattatcg cccgagaaaa cgttaaagcc
    87301 ctgcgtaaaa acgttttggc gaaatgttac ggcggcgata ttacgcgtaa gaaaaaactg
    87361 cttgaaaaac aaaaagcagg caaacgccgt atgaaacaag tgggcaacgt ggaaattccg
    87421 caaagcgcgt tcttggcgat tttgcaggtg agcgacaaat aatatttcgg cacatagggc
    87481 gggcgcgtcg ggaacacctc tcaaaccgcc ctgatttcag cggcacgcgc ccgcaaacaa
    87541 attttgcaat ttgccgcctt aaaacggttg cacggccggg cgggttttta accgttaagg
    87601 aacaacaatg aacacaatgc taatgtcggg cgcggctgcc gcgctgcttg ccggcatcat
    87661 cctttatttc aaaagcgaca agaaacggca ggaaaacggt gaatggagtt ccggcctcga
    87721 atacgcctat atcctgacgg cggtcggtgt gtttgccgct ttgtccctgt ttatgagctt
    87781 taccgccgtt ttcctgattt tcgttgtatt gtgcggtacg gcttgggggg tatataaata
    87841 ccgcctgaag actcatcccg aaatctcgga aagcagccac ttcggcgatt atttcggcag
    87901 tttcttccct accgttttgg tattgttcct catccggtcg tttattgccg aaccgttcca
    87961 aatcccgtcc agctcgatgc gcccgggatt aatcaagggc gatttcattt tggtcggcaa
    88021 attttcctac ggcctgcgcg tacccgtttt aaacaatgta ttcatcccca cgggcaaaat
    88081 cgaacggggc gatgtcgttg tttttaacta tcctttgcag ccggagatga cctacatcaa
    88141 gcgtattgtc ggcatcccgg gcgacattgt cgaatatcga gataaagttt tgacggtaaa
    88201 cggaaaaccg gcttccgaca ttcctgacgg cacataccgt tatcctgacg ataccgaccc
    88261 atccgaaatc cacaacacgg atatgttccg cagcggtctg gacggcaaat ccttcaatat
    88321 tctgaaaaaa gaaggacagc ctgccgtttc cctgcccgta ttgggcaaat atacctccga
    88381 tattatgtct gaaaacggat attccataga tcaaagcggt ctgaaccatt gccaatatgc
    88441 cgacgacggc agcggtttcg tgtgcaaagt tcccgaagga cgctatttcg ctatgggcga
    88501 caaccgcgac aacagtgccg attcgcgcta ctggggcttt gtggatgaca agctggttgt
    88561 cggcaaggca atgttcattt tgatgaactt cggcgatttc ggcagggccg gcacggcaat
    88621 ccgttaagcc ggaaaattac gccggaacat caaaaatgcc gtccgaaccc tgtttcagac
    88681 ggcattttta tcatcaaact ccttgtttcc ctaccggcag cctcggggat aatatagtgg
    88741 attaaatcta aaccagtaca gcctggcctc gccttgccgt actatttgta ctgtccgcgg
    88801 cttcgtcgcc ttggcctgat ttgaatttaa tccactatat gcaccgcctt gccggcatta
    88861 tcgatatggg cggcacatat ggattgcctg gaaaaatgat gcgttcgggc ggcgtatgat
    88921 ttgcctgtgt ttaaatcatc ggttgatttt atccgtcggc aagtgaaaag ccggttattt
    88981 ccgtttgtgt ggaaacccat cccattgaat aaagggcatt tcgtccgact gacggtgtgg
    89041 gcggcatatg gaaatacgga cattatttta gttgcattgc cattcctgcg gaggcggctt
    89101 ttttgattct gcccgccccg ataccgtctg aaagggcttc agacggcatt ttccgctccg
    89161 cgccgttgtc ggcatgtttg gtcttgccgg ttttgactgc ctgccagtcc aatcgtacgc
    89221 aggtttgcca cggcagaaaa tctgatgccg accggagtca aagctcggac ggctgtttca
    89281 gcgaaccgag gtaaatcgcc gaaagcgtca gcaccacccc tgcccactgc acgccttcaa
    89341 tcgttttgcc caatccgaaa taatcgatga acagggcggc gaccggttcg gacaaaagca
    89401 acagccccgt cagcgacagg gaaagcagcg gaatcgcata ggcaaccatc gcccacgcaa
    89461 aacactgcat caccacgccc cgtacacaag caccaaaccc gaatctttcc aagtggtcgg
    89521 ataaagtgcg ccgccgtcca tcagcaatgc cggaacaatc ggcgatgccg cgccgcccaa
    89581 actcaaaatc atcattgaag ggaaaagcgc cacctgttcg acttcatgag tcttgcggac
    89641 gaacaccata gatagggcaa gcatcagccc ggacaccaaa ccgctggcga atccccaaac
    89701 cgcattaccg ttgtagccga attccgcacc ggcaatcatc gccacgccca caaccgccga
    89761 catcaagctt gccgcctgca gcctgcccaa acgctcgccg aaaaagaaaa caccgattgc
    89821 cgacaagaaa aagatttgca ggctgttgag cagggtggaa atacccggcc cgaccgcgtg
    89881 tatgctttcg tgccaaagtg ccaaatcgaa agcgagaaac acgcccgccg tcagggcata
    89941 tcggacggtt tttctgtttt ttgggaattt ttgtccgaaa aaccgtgcca aaaaccaaaa
    90001 taccaatacc gaaatcagca accgccaaaa tgcgattgca tacgggccga cggggacgga
    90061 tctgacaatc aggctgccca atccgaacac cgcgcaaccc agcaccagca atggtgcggc
    90121 atattttttt ccgttaccga ccacaatatc ccctcccttc cccatatggc tgccgcgccg
    90181 ccggcaaaag tgccgccatt ccgaaaatat gccgtctgaa gcccaaagcc ccggctgcgc
    90241 ccggacaaac ggctgacttt acgggcaggc cggctatttt ccgttggggc aaaatccgtc
    90301 ctgccggata aaacccgaat aaaaaatatt tccctcaagc aatgcggcgg cgccaacacc
    90361 tgattaaaac acaaccggcg ttttcggtat atatgcaaaa acgcattttc cggcacggcg
    90421 ttacaccgat accgaaaaat gcgtctgaat gtttcgacaa tccgattaaa gcattatgcc
    90481 aaatctgcaa aaagtggcgt agagagatag cgttcgccat aagaaggcag cagcacgact
    90541 atcagcttgc cttcgttttc aggctgtttg gcaagctgca acgcgctcca aaccgccgca
    90601 ccggaagaaa tgcccgccaa aatgccttct ttttccgcca ttgcacgggc ggtttcaaaa
    90661 gccgcttcgt tcggcacttt ggcaatgctg tcgtagattt tggtattcaa aacggtcgga
    90721 ataaaacctg cgccgatacc ttgaatcggg tgcggacctt tttcgccgcc gctcaatacg
    90781 ggggaagcct caggctcgac ggcaaccact ttaacttcgg gtttgtattt tttcaatact
    90841 tcgcccacgc cggtaatcgt accgcccgtg ccgacgccgg caacgaagac atcgacttta
    90901 ccgtcggtat cgttccaaat ttcctcggcg gttgttttgc ggtggacttc gggatttgcc
    90961 tcattgtcga actggcgcgg cataaaataa gtgtctggat gagcgtccac caaggattgc
    91021 gctttggcaa tcgcgcccgc cataccttcg gcggcggggg ttagaatcag ttccgcgcca
    91081 aacgtgcgca acagcatttt gcgctctttg ctcatgcttt ccggcatggt aatcgccagt
    91141 ttgtagccgc gtgcggcaca taccattgcc aaaccgatac ccgtattgcc gctggttgct
    91201 tcgacaatga cggtgttttt gttgattttt cccgcttttt cggcggcctc gatcattgct
    91261 tcggcaatgc ggtctttgac gctgctgccc ggattaaaaa attccagttt cacggcaacc
    91321 tctgccttca aacctttggt caaacggttc agtttgacca aaggcgtgtt gccgatcaat
    91381 tcggtgatgc tgtttgcaat tttcatcttc tgctcctgaa taataaggtt tcgacagtaa
    91441 ggcgcaatcc ccatcggtat cctgtccgat tgcaggggat ttgcgccgaa ccataaacaa
    91501 atttcggata tggctttatc ctaaacctat gccgcctaaa aaaccaatac ccaaaattta
    91561 tttctttata actttttgtt ccgatacaga cggcatcaca atcatctgcc gatttcggca
    91621 agcagcatcg ccgttaccgc aacctgcatc aacatcgccg ccaacacgcc gagtcgcagc
    91681 tgcgaaccgc cgaacgttga cggcggctgc cggtctatac cgcagcggtg gacataggca
    91741 accgccatca atacaaactg caacacgaac caataaaccg gcttcagcgc ataaagtttg
    91801 agcaatgccg caaacacaaa cacttgaacc gttcccgaaa agcaataatg caccaacgca
    91861 catattccga caaaagcaag cgtcatcgat acattactga cggcaaaaag cccgagcagc
    91921 ggatgttcgg ggtctgcctg ccatccgttt ccatctgttt tccggttcca atacccgatg
    91981 aaaaaaaata tgctgcccaa agtcaggtaa aaatggggga tgaacaaagg cgcggcgcgg
    92041 gtcattcccc acatccccgg catcagcttt acccctaaaa ccgagatgcc gagccacaac
    92101 gcaatactcg cccaaaacca ttgcaacatt cctgcgcgga atatcagaaa cagaaaaacc
    92161 gccgcataca gtaaggcaga caaaaaatcc aaatgcagca aaatcatgaa tacgccaact
    92221 cgccttcaaa cacggtttcc gcagggccgg tcatcatcac atcgccgccg caggcccatt
    92281 cgatatataa agtcccgccc ggcaaaacca cctctaccgt tttcccttca tccaacagcc
    92341 ccagacggat acccgccacc acagccgcac acgcgcccgt accgcacgct tgggtttcgc
    92401 ccacgccgcg ctcgaacacg cgcaaacgga ttgccgtccg tccgacaacc tgcataaagc
    92461 cgacgttgac gcgttcggga aactgcctgt gcggttcgat acgcgaaccg gtttcgcgca
    92521 ccggcgcgca ttccacgtca tcgactacaa tcacagcatg aggattgccc atattgacgc
    92581 agctgacagg ctgaatgccg gattcgagat gcaccccgta aatacaggca tcatccccct
    92641 cgcccgattc ggggacaaac ggtatttcag acggcataaa cttcggtttg cccatattga
    92701 ccgtaaccat accgttatcg gacaatttcg gaaaaataat gcccttggcc gtttcaacac
    92761 aaatttcttt cttatcggtc aaacccttgt ctgcaacaaa acgggcaaaa caacgcgctc
    92821 cgttgccgca ttgcccgacc tcgctaccgt cggcattgaa aatacggtaa cggaaatcca
    92881 cgccttcggt ttccgaacac ccgaccacca aaagctggtc gaagcccacg ccccggaagc
    92941 ggtccgccca tgccgcaatc ggcacgtcct cgggggtaaa atcctgactg accgcgtcaa
    93001 tcaccataaa atcgttgccc aaaccgtgca ttttggtaaa tttcaaagtt ttcatattgt
    93061 tccgaaatcc cgtattgtct gttgcttccg aatccgaaat ccccgagccc gaagggaaat
    93121 gcttccatct cccgccgcct gaggattaaa atgagccatt atactgtttg ttacccctta
    93181 caagaaacac aatgtcccgc ctgttcctgc tgcttttccc ttgggcaacc cttgccgcct
    93241 gcacccgaaa cataccggca agccccgccg tttcaaattg cgcgcatctt cacaaccgca
    93301 ccgtcctgta ttttgacgca cacggcaaac ccgccgacag cacagcaaag ggcggatttt
    93361 acaggaccgt cgaaattcat gacacaaacc gcatccttat tcaagacttt aacagcacgg
    93421 ggaatgccct cgtcaaaccc tattggtcgg actgccggaa attgtccgtt ttccccgaca
    93481 aacatctgac catgagccat ccatgaacca cgacatcact ttcctcaccc tgttcctgct
    93541 cggtttcttc ggcggaactc actgcatcgg tatgtgcggc ggattaagca gcgcgtttgc
    93601 gctccaactc cccccgcata tcaaccgctt ttggctgatt ctgctgctta acacaggacg
    93661 gataagcagc tatacggcaa tcggcctgat gctcggatta atcggacaac tcggcatttc
    93721 actcgaccaa acccgcgtcc tgcaaaatat tttatacaca gcctccaacc tcctgctgct
    93781 ctttttaggc ttatacttga gcggtatttc ttccttggcg gcaaaaatcg agaaaatcgg
    93841 caaaccgata tggcgcaacc tgaacccgat actcaaccgg ctgctgccca taaaatccat
    93901 acccgcctgc cttgctgtcg gaatattatg gggctggctg ccgtgcggac tggtttacag
    93961 cgcatcactt tacgcgctgg gaagcggtag tgcgacaacc ggcggactgt atatgcttgc
    94021 ctttgcactg ggtacgctgc ccaatctttt ggcaatcggc attttttccc tgcaactgaa
    94081 aaaaatcatg caaaaccgat atatccgcct gtgtacagga ttatccgtat cattatgggc
    94141 attatggaag cttgccgtcc tgtggctgta agccccattc attcccgata cccgtaaaaa
    94201 ataccgtctg aaacctgttc agacggtatt ttcaccctaa gcgttactcg gcggcatttt
    94261 cttcacggac aggcgcatcc agcagggctt tgatggacag acggacacga cctctgtcat
    94321 ccacttccaa tgctttcacg ttcaccacct gaccgacttg caggtaatcg ccgacattgc
    94381 gtacgcgctc gtgtgcgatt tggctgatgt gtaccaaacc gtctttgccc ggcatcacgc
    94441 tgacaatcgc gccgacgttg ttgtcgagga ttttaaccac agtaccttcg tacactttgc
    94501 ccacttccac ttcggcagtg atttcttcga tgcgtttttt cgctgcatcg cccgcttctt
    94561 gagtagtcgc agcgatagtg atggtaccgt cttcagcgat attgatttca gtaccggttt
    94621 cagcggtaat cgaacgaatg gtttcgccgc ctttaccaat cacatcacgg attttgtctt
    94681 ggctgatttt catggtgaac aaacgcggcg cgtgtgcgga cagctcttgc ggacccgcaa
    94741 cggcagcttt catctgatcc aagatgtgca aacgcgcttc tttggcctgt gccaaagcaa
    94801 tttgcataat ctctttggta atgccttgga ttttgatgtc catttgcagc gcggtaacgc
    94861 cttcggtcgt accggccact ttaaagtcca tatcgcccaa gtggtcttcg tcgcccaaaa
    94921 tgtcggtcag gacggcaaac ttattgcctt ccaaaatcag acccatcgcg ataccggcaa
    94981 cgtgtgcttt caaaggcacg ccggcagaca gcaggctcaa acagccgccg cagacagaag
    95041 ccatagagga agagccgttg gattcggtaa tttcggagac cacgcgcatg gtgtagctga
    95101 aatcttcagg tttcggcaat acggccaaca acgcacgttt agctaaacgg ccgtgaccga
    95161 tttcacggcg tttcggcgcg cccatgcggc ccacttcgcc ggtagagtac ggcggaaagt
    95221 tgtagtgcag cataaagcgg tcggtatatt cgccggacaa cgcgtcgatg atttgttcgt
    95281 cgcgcgaagt acccaaagtc gctacggcaa gggcttgggt ttcgccacgg gtaaacagtg
    95341 cggaaccgtg cgtgcgcggc aatacgccgg tttggatgtt cagcggacgg acggtgcggg
    95401 tgtcgcggcc gtcgatgcgc ggttgtccgt ccaaaatttg gctgcggacg acatcggctt
    95461 ccaagtgttt gaaaatacct ttgatttcgt tggctgccag cgtgtcggtt tcttcggtaa
    95521 tcaaggcttc ttttaccgca ttccaagcct cgtccaattt ggcggaacgc gcttgttttt
    95581 gacggatttt gaacgcttct ttaatggttt cgccggcaat cccgcgtact ttggcaacca
    95641 gttcttcatt ggtttcaggt gctttccaat cccaaacttc cggattcact tcgtcggcaa
    95701 attcattgat tgcattgatg gcaacctgca tttgatcgtg gccgtaaacc accgcgccca
    95761 acatcacgtc ttcgggcagg attttggctt cggattccac catcaacacg gctttggaag
    95821 taccggcgac caccaagtcc aattgtgatt tcgccaactc ggctttagtc gggttcaaaa
    95881 cgtacacgcc gtttatataa cccacgcgcg ccgcgccaat cgggccggcg aacggtacgc
    95941 cgctcaacac cagcgcggcg gacgcgccca acattgcagg aatgtcggaa tcgatttcgg
    96001 gatcgacgga cacaaccatc gctacgattt ggatgtcgtg gtagaaacct tcggggaaca
    96061 gcggacgaat cgggcggtcg atcagacggc tggtcaggat ttctttttcg ctttgtttgc
    96121 cttcgcgttt gaagaagccg ccggggattt tgcctgcggc ataagtgcgt tccaaataat
    96181 cgacggccaa cgggaagaag tcctgacctt ctttgacctc tttgttggtg gtaacggcaa
    96241 caaaaacaac agtgtcgccc atcgatactt taacggcagc ggcagcttgg cgggcaattt
    96301 cgccggtttc caaagtaacg gtttgattac cgtattggaa ggtcttaacg tatttattga
    96361 acatcattgt tcctttcaaa ataccgcact gctaaaacac taataatgca cactaaaatc
    96421 cgaatgtgca tagttagggt ttcagaccgt gcggcaggtt gtaaacaagc tttcagacgg
    96481 catttcggac gctgaaaaca attgcggatt ataaaggcaa ccatccttaa aatccagtat
    96541 tgatacaata aaaaggccgt ctgaaacata tccttttcag acggccttca gtcttcaaaa
    96601 tcaatcttgt gtcagtaaag tcaatgcttc ctgatatttc tcgaccgttt tccgaattac
    96661 atcggcaggc actttaggcg caggggcttt tttgttccaa ccgctttgtt ccagccagtc
    96721 gcggacgaat tgtttgtcaa aagacggcgg attggtgccg acttcatatt gatccgccgg
    96781 ccagaagcgg ctcgaatcgg gagtcaatac ctcatccatc agcgtcagcg tacctttttc
    96841 atccaaaccg aattcaaatt tggtatcgca aatgatgatg ccgcgcgatt tggcatattc
    96901 cgccgcttcg gtgtaaagcc gaaccgcttt gtcgcgcact tcttccgcca attctttacc
    96961 gataatgcgt ccgcattctt caaagctgat gttttcatcg tgatcgccga ctgcggcttt
    97021 agttgagggc gtaaaaatca cttcgggtag ttgttgcgct tcccgcatac cttcgggcag
    97081 ccgaataccg caaaccgagc cggttttttg ataatctttc caaccgctgc ctgccagata
    97141 accgcgcaca atggcctcca ctttcaccgg agtgagtttt ttagctacaa cggcgcgttt
    97201 ttccaaagct ttggcttcgt tttcaggcaa aacatcgtac acggtatcgc cggtgaaatg
    97261 gttgggcata atatgcgcca gttttttaaa ccaaaaattg gaaatctgcg tcaggatttc
    97321 gcccttaccc ggaatcgggg cgtccaaaat cacatcaaac gcggacaggc ggtcggaagc
    97381 gaccatcagc atacgtttat cgtcgatttc atataaatcg cgcacttttc cggaataaat
    97441 ctttaccaaa ccaatctcac tcatttcgca cccccctgaa aatatcttga aaataccgac
    97501 ccgacaggtt tgaatcgcaa accgatattc tagccgaagt cggcgcaaaa caatacccat
    97561 ggcacaaaaa gccaacccgt caaccgtcgg caaagttttg gcactataat accgacagca
    97621 agtcctacaa tacactttca ccaaaggaaa tacctcatga gaatcctatt gacaggctcg
    97681 aaaagccaac tggcacactg cctgcgcgac cgtcttcctg aagactggga aaccattgcg
    97741 acggattccg catccctaga cattaccgat gccgatgccg tctgcaacat ggtcaaaagt
    97801 ttccaacccg acgctattgt caacacggct gcctatactg ccgtcgacaa agcggaaggc
    97861 gatgcggcag cggcatttgc cgtcaatgct tccgccgttt acaaccttgc cttggcagca
    97921 catcgcgccc atgcccgatt catccacatc tcaaccgact atgtctttga cggaaaaggg
    97981 aaaataccct atcaggaaag cgactttacc aacccttcca atgtatacgg acaatccaaa
    98041 accgccggcg aactgctcgc actgtctgcc aatcccgaca gccttatcct gcggacttct
    98101 tggctgttta gcgaatacgg ggacaacttt gtccgcacga tgctgaacct tgcgcgggaa
    98161 cgttccccgc tgtccgccgt ccacaaccaa atcggctgcc cgacctatgc cggcgacttg
    98221 tccgccgcca tcatccgcct gttgcagcag tccaatcccg ttcgcggcat ttaccactac
    98281 gccggcggca aatccgtatc ctggtacgaa tttgcccggc acatctttca gacggcattg
    98341 caacaggatg cctcttttcc cgttcccgaa ctgaaagccg tttcggatga aggctctgcg
    98401 gctgccgccc ccaggcccgc atacagcatt ttggactgcc gcaaaatcga aaacgacttc
    98461 ggcatcaaac cgtcagactg gcaaaaagcc cttgcacagg tcgtttccaa gctgctctga
    98521 tgccgcccgc ccctccgttt cctccgccaa gcaccgcctt ggcggttttc ttataaaatc
    98581 tactgattac acaaacacat atcatcttta cacaatcatg cttccatcaa cagcaaaaca
    98641 tgatatgatt gccaacaatg acatctgaca ataaattttc taatttttat tgaaaaaatc
    98701 aataaattaa gaattctccc aaccgacaaa taaaataaag aaagggtcaa tatgcaacac
    98761 cgtagaagat tggcaattta ccaggcatcc aaacgtgctt cctttaccgg caggtcatca
    98821 gccccacaaa aacgtaaaga acgttgattt gaaaaaaatg ccgtctgaag tcctgcttca
    98881 gacggcattt tttaccgttc gagaaactgt ttcaacctgt cctcatccaa aaaccagtgg
    98941 caaatttcct catctcccgc caacaaaacg ggaatcagct cattgtattt ttcttccaaa
    99001 acaggatttt catccacatc gaccacttcc agcccgaacc cgtattcatc ctgaaaaggt
    99061 ttgagtgcat cgcgcatttt gtggcacaag ctgcaatatt cacgaaacat caaggtcaat
    99121 ttcatccgcg tttccttatc tgtcaatttg cacacgccaa agccttagac gcagcagaat
    99181 catggtctat ttgggaaaaa acaatgtctt cgaggaagat gatactcaag tcctgccaaa
    99241 aacagcaaaa atgccgtctg aacagttcag acggcatttc caaaaccgtt ttacgcttga
    99301 acgttgatac ccgccgtaac cgtcggttgc gcattgtgcg ccgtcagaaa cgaagcggca
    99361 agctccccgc agcagccgca gcagctcgcc acgcccaatt gcgactgcaa atcgcccatt
    99421 gtggtcgcgc cggcagcgat ggtttccttg atttgatggt cggtaacggc attgcagatg
    99481 cagacaaaca ttttgtgctc cgtgtgttct caaactatcg taccgatagc ggcttttatt
    99541 atcgtatgca aatataaata aaaacggttc gcattgcaag gtcggcatac acggtttgtc
    99601 ttggtaattt tttatcaagt ttgcattttc gattgatttc ttattaaacc gaagcagaag
    99661 cctcagtttc gggaatggtg ttccttaacc acgctatgca gccgcacatt ggcaacatgg
    99721 gtataaatct gagtcgtgtt caaatcggcg tgtcccaaca tatcctgaac tacgcgcaaa
    99781 tccaatccgt gccgcaccag atgcgtggca aaggcgtggc gcaggctgtg cgggctgatg
    99841 tgcccgatgc ctgccctgcc ggcatattct ttgacaatca tccacgccaa ctgccgggaa
    99901 atgcccgttt ttttctgact gacaaacaat gcgtcgcaac tcctgccttt cagcagaagc
    99961 gggcgtgcct ccgtataata gcgttccacc caatacgctg actcctgtcc catcgggacc
   100021 atcctctgct tgtcgccctt gcccagcgtg gtaatacaac ccctgtccaa atccacattg
   100081 ccgaaattca gcccgaccgc ctcgctgacg cgcaagccgg tcgcgtacat caattcgagc
   100141 agggccttat cccgcaaacc gtgcggcgtg tcggtatccg gtgcggcaag cagtcgggaa
   100201 atctgctgtt ctgtaatcag tgtcgggatg tttctgtcga tttttggcgg tttcagcagg
   100261 cgggtgggat tgtccgccct catcccttcc cgttccatcc acacatacag gcgtttgcac
   100321 gccgacagtg cgcgtgcctg cgaactccgt tgctccccgt caacataaac cgccgccgcc
   100381 aaatctgctt cgtccgcatc cttcagcgtt ctgccgcaca aagacagccg gcgggcgatc
   100441 ttttccaaat cccgccggta gctgtccaaa gtattccgac tgagccgccg atccaaccac
   100501 aaagtttcca gcagcctgtc aatcaaaccg ttttccataa cgttccaaac caatgccgtc
   100561 tgaatcttct tcagacggca tggttcacat catcgggaaa gcgtttccaa cacttcctgt
   100621 gcatgacccg ccactttgac tttccgccac tcccggacaa tttcgccgtc cttgttcaga
   100681 acgaacgtac tgcgctcgat gcctaacgac tctttcccat acagtttttt caatttgatg
   100741 acatcaaaca ggcggcacac cgtttcatcc ttgtcgctca acagctcgaa ccggaaaccc
   100801 tgcttggcgc aaaaattctg atgcgccttt acgccgtcgc gggaaatacc gaccacggta
   100861 taacccaatg cctcaaactg ttccaaacgc gcattaaaat ccaagccttc cgtcgtacag
   100921 cccggcgtac tgtctttcgg ataaaaatac acgaccaaag gcagatgttc tgccgaatga
   100981 aaatccgcac cgctgctcga aggcagggta aattcatatt tcatatccat agtcctactc
   101041 ccgatattcc cattattcaa aacggcacgc agacgcaccg ccgcaattgc caaaccaacc
   101101 ccgattctac cgccccaaag gacaaggatt caaccgccgg aaacatccaa accgacacac
   101161 gacggcatga aaaatatcca tgtcaaacca caaaatatgt tccgatttaa aaacagaatg
   101221 ttataaaacc caactccaaa aacacaacaa gaccgcccgc ctacgggcag tctcctgtca
   101281 gacgacatac tttacagatg gctgtttttt caacaaaata acgccaatac tcaaaaatac
   101341 ggaaccaaaa atgtccatcc gcaccctgaa acgcctgccc tcatcgctgc tgctcggcct
   101401 ctgcctttcc ctgccgtcag cccacctttt tgccgacaac gacattttag ggcaattttt
   101461 agaacagaac atgcttacct cctccgatcc gatagaaata ttcgccgaaa gcacgatata
   101521 tcccgccgat acccaggcca ttacgggcgg gctgattctg tcctcccaat ccgcactggt
   101581 cgtcaacaac aaaacagggc aaatactgta tcagaaaaac gccgacagga ttatgcccat
   101641 tgcctccatt tccaagctca tgagcgcgat ggtcgttttg gatgcaaact tggacatgaa
   101701 cgaaaccgtt accatcacac ccgacgaaat cgaccgcctc aaagggaccg gcagccgtct
   101761 tgccataggt acggcactta cacgtaaaga actgctgcac cttagcctga tgagcagcga
   101821 aaaccgcgcc acccatgcat tgggcagaac ctaccccggc ggcatgggcg catttgtcgc
   101881 cgccatgaac cgcaaagccc aaagcctggg catgtacggc agccgctttt acgaaccgac
   101941 cggactcaac ttccaaaacg tttccaccgc caaagacctg agcctcatgg tcaacgccgc
   102001 cgcccaatat ccgcaaatcc gcaccaactc gacttccaac tacgcctcgg tacaaaccaa
   102061 aaacgggcag caaaactata aaaactccaa tgccctggtc agggaaggca tgtggaacat
   102121 cgaattgcag aaaaccggct acatccgcga ggcaggcagg tctatggttg tcaaagccaa
   102181 cattcaaaac caacccgtta ccatcgtatt gctgaactcg cccacatccg ccacacgcgt
   102241 caacgacgcc cgcaaaatcg aatcgtggat gctgcagcaa cgctcctgac atacaaatgc
   102301 ccggcggaaa accgcccaaa cacaaaaaaa ttggtaattt ttcctattcc aggctacaat
   102361 tcgacacgca cagggcatct gcccgatttc acaatcataa tagcggagtt aaaaaatatg
   102421 acagctaaag gacaaatgtt gcaagatccc ttcctgaacg cattgcgtaa agagcatgtt
   102481 ccggtttcga tttacttagt taacggtatc aaattgcaag gtcaggtcga atctttcgac
   102541 caatacgtcg ttcttctgag aaacacttcc gtaacccaaa tggtttacaa acacgccatt
   102601 tccaccatcg taccggctcg ctccgtcaac ctccaacatg aaaacaaacc ccaagccgca
   102661 ccggcttcga cccttgtcca agtggaaacc gtccagcagc ctgccgaata atccgcacga
   102721 agcatgacgt gtcatatctt tcaatacctt accggacaac ggtaaggtat ttttattttc
   102781 aggcggcatt taaaaatatt attgaaaaac atccttccat tcgcccattg ccttttgcgg
   102841 gaggcacttc ccgaaggcgg caatgcgctg gacggcaccg ccggcaacgg acacgacacc
   102901 cttttcctcg cacaaaccgc aggcaacagg gggaaagtgt gggcattcga catccagccg
   102961 caagccctga acaacacccg atgccgtctg caggaagcag gttacagcaa tgtacggctc
   103021 atcttggacg gacatgaaaa cctgaagcaa tatattccgg agccgctgga tgcagccgtt
   103081 ttcaatttcg gctggttgcc cggaggcgac aagaatctga ccaccgacaa ggaaaccagc
   103141 attcccgccc tttgagcaac cctctccctg ctgaaagaag acggtatgct tattgccgtc
   103201 ctctatccgg gacacgaaaa cggcaaacag gaggcagaag ccgtcggaca atgggcaaaa
   103261 aacctgcctc aagaacagtt tgccgtttta cgttacggct ttaccaaccg gaagaatagc
   103321 ccaccctatc ttttggcatt tgaaaaactg cgtcaaaaat aactgtttgc ggtaaaataa
   103381 gccgattcta acccttattt tgatacgagc cgaccatgca gtatctgatt gtcaaataca
   103441 gccatcaaat cttcgttacc atcaccattt tggtattcaa catccgtttt ttcctacttt
   103501 ggaaaaatcc agaaaagccc ttggtcggct tttggaaagc actgccccac ctcaacgaca
   103561 cgatgctgct gtttacggga ttgtggctga tgaagattac ccatttctcc ccgttcaacg
   103621 cgccttggct cggcacaaaa atcctgctcc tgttcgccta catcgcactg ggcatggtaa
   103681 tgatgcgcgc ccgtccgcgt tcgaccaagt tctacaccgt ttacctgctc gctatgtgtt
   103741 gcatcgcctg catcgtttac cttgccaaaa ccaaagtcct gccattctga aacactgcca
   103801 tgaacaacag acattttgcc gtcatcgcct tgggcagcaa ccttgacaac cccgcacaac
   103861 aaatacgcgg cgcattagac gcgctctcgt cccatcctga catccggctt gaacaggctt
   103921 cctcactgta tatgaccgca cccgtcggtt acgacaatca gcccgatttc atcaatgccg
   103981 tctgcaccgt ttccaccacc ttggacggca ttgccctgct tgccgaactc aaccgtatcg
   104041 aagccgattt cggacgcgaa cgcagtttcc gcaatgcacc gcgcacattg gatttggaca
   104101 ttatcgactt tgacggcatc tccagcgacg acccccgcct taccctgccg catccgcgcg
   104161 cgcacgaacg cagtttcgtc atacgccctt tggcagaaat cctccctgat tttattttgg
   104221 gaaaatacgg aaaggttgtc gaattgtcaa aacggctggg caatcaaggc atccgtcttt
   104281 taccggacag gtaattccgc acgcggatgc cgtccgaaca ccggtttcag acggcatttt
   104341 tcctttgccg ccaacacgcg tgcaaaaaaa atcgcccctt gagaaagggg cgcaaaagga
   104401 acacaaacca ctaccaaaac tttaaatctg caacactgcc tgccgcatac tgtacccgac
   104461 aggatataag gccctcacta aatcgtttcg agaaatccaa acttcttcat caccgacaga
   104521 aaatctgcct ttctccggca ccagctccaa caaaaacggc tgaaccgccg tatgcagctt
   104581 gtccttacac cgccccagct ttctggacaa cgcggacagg gcgcgtttgt aggcattatc
   104641 cttgcggtcg agctccctcg cactaccgac ccagctcaaa cgaaggttgc ggctttcatc
   104701 ttcaatcaac aaaatacctg tttcaacttc ccccacctcg ggttcgcacg aaagcgcaat
   104761 ataatatttg ccgtcaatat tgacatatgc cgcttcatgc acctgacagt tgcaacccga
   104821 ggttgcgagc agggaataaa cagaatcacg gtgcagcctc aaaatatcgg aggaaatctg
   104881 tttgaaaata ctttccggca tttttccctc ctttcgtctg ctgcgttgtt tactggattg
   104941 actgcattat acagacatct cccaatttta caaatgataa ttgttcctat tttttaaaaa
   105001 gtttataaat tattgataaa tatatgaatt aatttttaag atttaaaacc tttgtctcaa
   105061 aaaatacaaa aatgccgtct gaaacgtgtt cagacggcat tggcttcaca ggcagtccgt
   105121 ttaaaatttc acgcccttat gcagcgcgac gatgcccgca ctcatattgt gataatccac
   105181 gctgtcgaag cctgcatcca gcatcatctg tttcaaagtt tcctgatcgg ggtgcatacg
   105241 gatggattcg gcgagatact ggtagctgtc cgcatctttg gcaatcagcc tgcccatcac
   105301 cggcaacagc ttgaaagaat aaaaatcgta tgcgccttcg agcggcttgt agattttgga
   105361 aaactccaac accagcaacg taccgcccgg tttcaaaaca cggtacattt ccttaagcgc
   105421 ggcatcttta tacgtcatgt tccgcaagcc gaacgccacg gagaccaagt tgaaataatt
   105481 gtcggggaaa ggcagttttt ccgcatcggc aagcgacacg ggtaaaatca tgccttcgtt
   105541 caacagacgg tcgcgcccga cggtcagcat agaagaatta atatcggtca gccaaacctc
   105601 gccttccttg ccgacccgtt tcgcccaacc gcgcgacaaa tcgcccgtac cgcccgcaat
   105661 gtccaacact ttatcgcctt ttttcaggtg cgccgtatgg atggtaaaat gcttccatac
   105721 gcggtgcagc cctgccgaca tcacatcatt cataatgtcg tagtttttgg cgacggagtg
   105781 aaacacttcc gccacttttc cggctttctc atcttcgttg accgtgctga acccgaaatg
   105841 cgttttttgt ccgcccatat tctaaccacc tccctgatac cgccctacgg catcccaaac
   105901 ttaacaatct gattgctttt caaaacgcga cgcgcccgcc gccgccaatt tgtccaaata
   105961 ttcctgccac atcgcatcgt atccgtatgc cagtctgtgc agatacgccc aatcgtaaag
   106021 accgctgtcg tgcccgtccg aaaaactgat tttcagcgca tactgtccga caggctgcaa
   106081 atccgtaatc tgcacatccg ccttaccggt ctgcaaaaca tcctgtccca cgccgtgtcc
   106141 gcgcacttcc gcactcggcg aatagacgcg taaaaattcg gcaggcagat ttttaggttc
   106201 gtccccataa gtcaaaacca acacggcacg gtttttcaac aaacggattt caacaggaat
   106261 attattcaaa aaacacctcg gttcggcata caatatccgg caggcgtacg cgtgccttta
   106321 aacggcgcgt attttaacag ctttatcccg ataccgatat tcaaacccac acttcagacg
   106381 gcattgatat ggacaatctc gtatgggacg gcattcccga catccgcaca ctcgaccaaa
   106441 ccatccgcaa acacgcacac ccgctcaacc tgattgtctg cctccccgat aatcagattc
   106501 ccgattttca aaccgcacaa gatgcttcgg actcggaatg ccgtctgaag caccgtttgg
   106561 atcaggcaac ccagtgcctc cagttcgaca gcatcaacct catcgaacac atcctgcccg
   106621 atgtccgctt ctggctggtt cccccttcac gcacccgccg cctgcacgaa cacttccacc
   106681 acatttcctg gcagaccgaa gccatcccgc aaaccgaaag caagtccgac aaaccctggt
   106741 ttgcacttcc acaaacatcc gaacggaaaa aaccggaaca cgtcctcgtc atcggtgcag
   106801 gcattgccgg cgcatcgacc gcccacgcct tagcatcaca cggcatttcc gttaccgtat
   106861 tggaagcccg aaaagccgct caagccgcca gcggcaaccg gcaagggctg ctttacgcca
   106921 aaatctcgcc gcacgacacc ggacagaccg aactgctgct tgccggctac ggctacacca
   106981 aacgcctgct cggacacatc ctgcccgact ccgacacttg gggcggcaac ggcatcatcc
   107041 acctcaatta cagccgcacc gaacaacaac gcaatcacga attgggtttg caaaaacacc
   107101 ataaccacct ctaccgcagc atcacgtctg cagaagccga aaaaatcgcc ggcatcccgc
   107161 tgaacacgcc ctacgccgaa ccattatgcg gactctactg gcaacacggc gtatggctca
   107221 atccgcccgc attcgtccgc accctcctca gccatccgct gatcgaacta tatgaaaaca
   107281 caacgttaac cggcatttcc cacgacggag aaaagtggat tgcaagcacg ccaaacggca
   107341 catttaccgc cacacacatc atctactgca ccggcgcgca cagcccctgc ctgcccgaaa
   107401 ccaacctcgc cgccctaccc ctcaggcaaa tacgcggaca aaccggactc acaccgtcca
   107461 ccccgttttc cgaacaactg cgttgcgccg tttcaggcga aagctacatc agcccgtcgt
   107521 ggcacggact gcactgctac ggcgcgagtt ttattcccaa cagcagcaat accggatgga
   107581 acgaagccga agaagcctca aaccgccaag cattggcaca ccttaacccc gcccttgccg
   107641 aatcattgtt tgccgccaac ccaaaccccc aaaaacacca agggcacgcc gccatacgct
   107701 gcgacagccc cgaccacctc cccgtcgtcg gcgcactcgg cgacattgcc gccatgcggc
   107761 agacttacgc caaacttgca ctggacaaaa actaccgcat cgacaccaca tgcccatatc
   107821 tgcccaatgc ctacaccaat accgcccacg gcacacgcgg gcttgccacc gcccccatct
   107881 gcgccgccgc cgttgccgcc gaaatcctag gcttgcccca tctcttttca caacgcctgc
   107941 gccacgccct acaccccaac cgcaccgtca tccgcgccat cgtcagaagg cagaacctga
   108001 ttccttaagc ctttgccgca tgccgtcccg accacaaaaa atcatattca aataaacata
   108061 ccaatcctga cggtaaaata tcgaaaacac ccataacgtt ttcttcccaa aataaggagt
   108121 aaggaatgcc taacaaattg cctttgctgt ccaagatgaa ttttccgtaa cattcgaaga
   108181 cttccttaac cctgccagcc gcttcggttt ctgtaccccc tgttagataa ggaagggatg
   108241 aaaaagccct gttacttgta tgaagcaaag ggcgttaatt tttgttaatc ttcccttctc
   108301 aggaactcaa tatataagga tgttaatttt tatttccctt ctcgaggact caatag
//
LOCUS       DAWTQS010000002        72881 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.002, whole genome shotgun sequence.
ACCESSION   DAWTQS010000002 DAWTQS010000000
VERSION     DAWTQS010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 72881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72881
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.002"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(<1..2343)
                     /gene="rnr"
                     /locus_tag="WNA25_000113"
     CDS             complement(<1..2343)
                     /gene="rnr"
                     /locus_tag="WNA25_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFA9822988.1"
                     /translation="MNKNIKSLNLREKDPFLSREKQRYEHPLPSREWIIELLERKGVP
                     SKIESLARELSITEDEYVFFERRLKAMARDGQVLINRRGAVCAADKLDLVKCRVEAHK
                     DGFGFAVPLMPMDEGDFVLYERQMRGVMHGDTVTVRPAGIDRRGRREGTVLDIVERAQ
                     SKVVGRFYMDRGVAILEPEDKRLNQSIVLEPDGVARFKPESGQVIVGKIEVYPEQNRP
                     AVAKIIEVLGDYADSGMEIEIAVRKHHLPHRFSEACAKSAKKIPDHVRKSDLKGRVDL
                     CDLPLVTIDGETARDFDDAVFAEKVGRNYRLVVAIADVSHYVRPDDAIDADAQERSTS
                     VYFPRRMIPMLPENLSNGICSLNPDVERLCMVCDMVVTYAGNIKEYRFYPAVMRSHAR
                     LTYNQVWKWLSDGIGNPHKAQIDTLYKLFKILQKKRLARGAVEFESVETQMLFDGNGK
                     IEKIVPVVRNDAHKLIEECMLAANVCAADFLLKNKHTALFRNHLGPTSEKLATLREQL
                     GLLGLQLGGGDNPSPKDYAALAEQFKGRPDAELLQVMMLRSMQQAVYEPHCEGHFGLA
                     YEAYAHFTSPIRRYPDLTVHRAIKAVLNRKTYTPNKSWQALGVHTSFCERRADDAGRD
                     VENWLKTYYMRDKVGEIFEGKISGVANFGIFVTLDDIHIDGLVHISDLGEDYFNFRPE
                     IMAIEGERSGIRFNMGDRVAVRVARADLDDGKIDFVLIAGGSGRRRKVKLSASAKPAG
                     AAGKGKSKTTAEKKTARCGKVRGRGVPAVAESGKKAKKPVP"
     gene            2432..2518
                     /locus_tag="WNA25_000114"
     tRNA            2432..2518
                     /locus_tag="WNA25_000114"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2466..2468,aa:Leu,seq:cag)
     gene            2557..2647
                     /locus_tag="WNA25_000115"
     tRNA            2557..2647
                     /locus_tag="WNA25_000115"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2591..2593,aa:Ser,seq:gga)
     gene            complement(3044..4855)
                     /gene="typA"
                     /locus_tag="WNA25_000116"
     CDS             complement(3044..4855)
                     /gene="typA"
                     /locus_tag="WNA25_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFA9822989.1"
                     /translation="MKQIRNIAIIAHVDHGKTTLVDQLLRQSGTFRANQQVDERVMDS
                     NDLEKERGITILAKNTAIDYEGCHINIVDTPGHADFGGEVERVLGMVDCVVLLVDAQE
                     GPMPQTRFVTKKALALGLKPIVVINKIDKPSARPSWVIDQTFELFDNLGATDEQLDFP
                     IVYASGLSGFAKLEETDESSDMRPLFDTILKYTPAPSGSADEPLQLQISQLDYDNYTG
                     RLGIGRILNGRIKPGQTVAVMNHEQQIAQGRINQLLGFKGLERVPLEEAEAGDIVIIS
                     GIEDIGIGVTITDKDNPKGLPMLRVDEPTLTMDFMVNTSPLAGTEGKFVTSRQIRDRL
                     QKELLTNVALRVEDTADADVFRVSGRGELHLTILLENMRREGYELAVGKPRVVYRDID
                     GQKCEPYENLTVDVPDDNQGAVMEELGRRRGELTNMESDGNGRTRLEYHIPARGLIGF
                     QGEFMTLTRGVGLMSHVFDDYAPVKPDMPGRHNGVLVSQEQGEAVAYALWNLEDRGRM
                     FVSPNDKIYEGMIIGIHSRDNDLVVNPLKGKKLTNIRASGTDEAVRLTTPIKLTLEGA
                     VEFIDDDELVEITPQSIRLRKRYLSELERRRHFKKLD"
     gene            complement(4972..5862)
                     /locus_tag="WNA25_000117"
     CDS             complement(4972..5862)
                     /locus_tag="WNA25_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFA9822990.1"
                     /translation="MFPVFHLSGESRRRMLQTALRFPHVFKARAEDSHKGTFGTLAVV
                     GGSAGMSGAPVLAASAAMYLGCGKVRAGFNQDTLPFAVIAGFPEIMLDTADGLTKRQG
                     INAWTAGCGLGTDAAAVETVAAVLARNRDEAVVLDADALNILSTDAETRNLARGCKNL
                     ILTPHPAEAARLLGTTVAQVQADRTAAVRKIGAILGATVVLKGHKTLVAASDTEIYVN
                     ESGNAGLATAGSGDVLGGIIGSLLAQGVPVFEAACAGAWLHGAAADVIKESAGIAAGL
                     SAGEIAPAARWLRNWITESM"
     gene            5927..6583
                     /locus_tag="WNA25_000118"
     CDS             5927..6583
                     /locus_tag="WNA25_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HFA9822991.1"
                     /translation="MKNKTKVWDFPTRLFHWLLAASLPFMWYSAKAGGDMLQWHTRVG
                     LLVLFLLVFRLCWGIWGSDTARFSRFVRGWAGIRGYLKNGIPEHIQPGHNPLGALMVV
                     ALLAAVSFQVGTGLFAADENTFSTNGYLNHLVSEHTGSLIRKIHLNFFKLLAVFSAVH
                     IAAVAAYRIFKKKNLVRPMITGFKYIEGKTSIRFAGKAALAAALSVAALAAAAILLLS
                     "
     gene            7012..8094
                     /locus_tag="WNA25_000119"
     CDS             7012..8094
                     /locus_tag="WNA25_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylate transferase"
                     /protein_id="HFA9822992.1"
                     /translation="MNYFPIFANLSGRPVLVVGGGAVAARKISLLPKAGAEVGVAAKH
                     LNAELSALAAENKILWLAKEFRAEHIRTVFLIIAASSDQALNRRVFHLAESCQKPVNV
                     VGDRDHCSFIFPSVIDRDPVQIAVSSSGSAPVLARLLRERLEALLPPSLGDMAEISGR
                     WRDAGFYFPVQLVVRQDADKADGFRGYQGRIERGSVTVGQTVRIEPNGLTAEVSEIIA
                     PKGEVAQAFAGEAATIRLDRDIDVSRGDLFVDKNFPLAPQKHPEATLCWFDERPLNTA
                     RKYLLKHGTQTVSAKVGEIESVLDVRTLEQETGAESLKMNDIAKVRINLQKPVTATPY
                     AENTAAGSFILIDEATYGTVAAGMIL"
     gene            8162..8845
                     /locus_tag="WNA25_000120"
     CDS             8162..8845
                     /locus_tag="WNA25_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HFA9822993.1"
                     /translation="MSGLSGYAWAKAGNGASAGLPALQTALPAAEPFSVTVLSASQTG
                     NAKSVADKAADSLKAAGIQVRRAELKGYKAKNIAGERRLLLVTSTQGEGEPPEEAVVP
                     HKLLNGKKAPKLDKLQFAVLGLGDSSYPNFCRAGKDFDKRFEELGAKRLLERVDADLD
                     FAAAADGWTGRIVARLKEEAAKNRATPAPQATSPRRPSDGIGGQVLQGRPLSRRPAGQ
                     SENHRPPIR"
     gene            8850..9884
                     /locus_tag="WNA25_000121"
     CDS             8850..9884
                     /locus_tag="WNA25_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit alpha"
                     /protein_id="HFA9822994.1"
                     /translation="MRHIEIDLSGSDLHYLPGDALGVWFDNDPALVGEILDLLGIDPA
                     TEIQAGGKTLPVASALLSHFELTQNTPAFVKGYATFADDDELDRIAADNAVLQGFVQS
                     TPIAGVLHRFPAKLTAEQFAGLLRPLAPRLYSISSSQAEAGDEVHLTVGAVRFEHERR
                     ARSGGASGFFADRLEEDGTVRVFAERNDGFRLPEDSRKPIVMIGSGTGVAPFRAFVQQ
                     RAAENAEGRNWLIFGNPHFAADFLYQTEWQQFAKDGFLHRYDFAWSRDQEEKIYVQDK
                     IREQAEGLWQWLQEGAHIYVCGDAAKMAKEVEAALLDVIIGAGHSDEDGAEGYLDMLR
                     EEKRYQRDVY"
     gene            9911..11676
                     /gene="cysI"
                     /locus_tag="WNA25_000122"
                     /pseudo
     CDS             9911..11676
                     /gene="cysI"
                     /locus_tag="WNA25_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251317.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
     gene            11849..13708
                     /gene="ilvD"
                     /locus_tag="WNA25_000123"
     CDS             11849..13708
                     /gene="ilvD"
                     /locus_tag="WNA25_000123"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFA9822995.1"
                     /translation="MPEYRSKTSTHGRNMAGARALWRATGVMETDFGKPIIAVANSFT
                     QFVPGHVHLHNMGQLVAREIEKAGAIAKEFNTIAIDDGIAMGHSGMLYSLPSRDLIAD
                     SIEYMVNAHCADALVCISNCDKITPGMLIAAMRLNIPTIFVSGGPMEAGKVIGVANIQ
                     PERRLDLIDAMIESADDNVSNRQVEEVEQNACPTCGSCSGMFTANSMNCLTEALGLSL
                     PGNGSYLATHAGRKELFLEAGRMIVEITKRYYEQDDETVLPRSIATKKAFENAMTMDI
                     AMGGSTNTILHLLAVANEAGVDFKMADIDRLSRVVPCICKTAPNNHDYYMEDVHRAGG
                     IFAILKELDKAGKLHTDVYTIHAPTLKDAIEKWDVTNPENTRAIERFKAAPGGVRTTQ
                     AFSQNRIWKTLDLDREKGCIRDVAHAYSQDGGLAVLFGNIAERGCVVKTAGVDESILK
                     FTGRARVFESQEAAVEDILGNQIVAGNIVIIRYEGPKGGPGMQEMLYPTSYLKSKGLG
                     KACALLTDGRFSGGTSGLSIGHASPEAAEGGAIGLVHEGDTIEIDIPKRSIRLVISDE
                     ELAARRAEMEARGSKAWKPENRDRYVSAALRAYGAMATSADKGAVRDVSQIER"
     gene            13750..14052
                     /locus_tag="WNA25_000124"
     CDS             13750..14052
                     /locus_tag="WNA25_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9822996.1"
                     /translation="MIINKFIWAMAVFSAILALVIGSGFTALEYVKEPPAAPYAVSAP
                     KTAGVKPRRILPERNPPCCRLTVQTPRPFQTPAMRTDRIRLTERQQIPFPSSGSAA"
     gene            14063..14863
                     /locus_tag="WNA25_000125"
     CDS             14063..14863
                     /locus_tag="WNA25_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HFA9822997.1"
                     /translation="MTKAVRRKNVQTTKICYAFRTPSAYGQYLADAGFDYLSLANNHS
                     NGFGAQGITATAGNLDELNIKYSGIENRFETAILKKNGVRYGFVSFAPNLAAVKLNDY
                     AKFKKLIRKTKQKTDIVIVMFHGGAEGKQAEHLPFDTEIFYGENRGNVVEFARLAVDS
                     GADVVFGQGAHVTRAVELYHDRFISYSGGNFATYGISGIAPIFKIITDKQGRFVSGNI
                     IPITQVGDKIPKIDPEKTVIERIIYLNHSDFPNGNGLDVSPGGDITRR"
     gene            complement(14959..16011)
                     /gene="bioB"
                     /locus_tag="WNA25_000126"
     CDS             complement(14959..16011)
                     /gene="bioB"
                     /locus_tag="WNA25_000126"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFA9822998.1"
                     /translation="MTVSPVALRRKTECKPHPTARYWKKCDVEALFGLPFLELVYQAA
                     EVHRQNFNPREIQLSTLLSIKTGGCPEDCAYCPQSAHHNTNLGKEQMMDVDEIVEKAK
                     IAKSRGASRFCMGAAWRGPKPKDVETVSAIIKAVKGLGMETCGTFGMLEEGMAEDLKE
                     AGLDYYNHNLDTDPDRYNDIIHTRRHEDRMDTLGKVRNAGLKVCCGGIVGMNETRAER
                     AGLIASLANLDPQPESVPINRLVKVEGTPLADAEDLDWTEFVRTVSVARITMPQSYVR
                     LSAGRSNMPEAMQAMCFMAGANSIFYGDKLLTTGNPDEDGDRILMEKLNLYPLQFEPE
                     GEVAEVEKASGIKADY"
     gene            complement(16218..17594)
                     /gene="mpl"
                     /locus_tag="WNA25_000127"
     CDS             complement(16218..17594)
                     /gene="mpl"
                     /locus_tag="WNA25_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFA9822999.1"
                     /translation="MKHIHIIGIGGTFMGGIAAIAKEAGFKVSGCDAKMYPPMSTQLE
                     ALGIGVHEGFDAAQLEEFQADIYVIGNVARRGMDVVEAILNRGLPYISGPQWLAENVL
                     HHHWVLGVAGTHGKTTTASMLAWVLEYAGLAPGFLIGGVPENFGVSARLPQTPRQDPN
                     SKSPFFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFADLGAIQTQFHHL
                     VRTVPSEGLIVCNGQQQSLQDTLDKGCWTPVEKFGTGHGWQIGEVNADGSFDVLLDGK
                     KAGHVAWDLMGGHNRMNALAVIAAARHAGVDVQTACEALGAFKNVKRRMEIKGTANGI
                     TVYDDFAHHPTAIETTIQGLRQRVGGARILAVLEPRSNTMKLGTMKSALPASLKEADQ
                     VFCYAGGADWDVAEALAPLGCRLRVGKDFDTFVAEIVKNARTGDHILVMSNGGFGGIH
                     TKLLDALR"
     gene            complement(17656..17961)
                     /locus_tag="WNA25_000128"
     CDS             complement(17656..17961)
                     /locus_tag="WNA25_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HFA9823000.1"
                     /translation="MRVSKMIGSILLVAAVQTVFSANVYECRHNGKTSYSQTPGKHCT
                     NAGLGRDRVYSSVRPAVKDRAEDAGVGDYSDTVRDEAVQNPKGNAQKDGSDAGIRPH"
     gene            complement(17973..18326)
                     /locus_tag="WNA25_000129"
     CDS             complement(17973..18326)
                     /locus_tag="WNA25_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823001.1"
                     /translation="MKNCEFFYDPTRAIYDSGADYLTREKYRLVVIANSAWGLLLNLP
                     CYYDEVLEKRKIPFGKQEIDDDMDKVSALKRKFKDISEIKVGDGWEYPFNYEQGMKEL
                     DEVLLKYIPFFEEKQ"
     gene            complement(18588..18785)
                     /locus_tag="WNA25_000130"
     CDS             complement(18588..18785)
                     /locus_tag="WNA25_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823002.1"
                     /translation="MPSEKIYYGVLIFLCIASMLLSPFFYAGALKPKKAALRKDGQWK
                     LILSSNAVAAAVLFWIWWKWF"
     gene            complement(18795..19580)
                     /locus_tag="WNA25_000131"
     CDS             complement(18795..19580)
                     /locus_tag="WNA25_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HFA9823003.1"
                     /translation="MKHISSTNNEHIRHLHRLLSQGKFRRQYAQTVLEGVHLLQVFLQ
                     SGRKPVGVYIPEAKMPSEEVLKLTAVLPEDGIFSVSDGILKKISSLSCADDILTLIDI
                     PLGGTLPDKGDCAVLDGVQDPGNVGTVLRSAAAAGVGTVVLGRGCADAWSPKVLRAGM
                     GAHFLLDIYSQADLEIWLAHYEDRVFATALREEKQAVLYGEDLCEPTAWVFGNEGAGV
                     GKAVLDRADKCVRIPMHDATESLNVAMAATICLFEQMRQRAAY"
     gene            complement(19654..20964)
                     /gene="tilS"
                     /locus_tag="WNA25_000132"
     CDS             complement(19654..20964)
                     /gene="tilS"
                     /locus_tag="WNA25_000132"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFA9823004.1"
                     /translation="MLTLDAFEQCLKDCFPQGLNGKKTAVALSGGLDSVVLLHLLVCA
                     GKRAGFVPEALHIHHGLSPRADDWADFCRNYCDMLGVGLETVKVCVEKNGLGIEAAAR
                     QKRYAEFAEKGFDVLALAHHRDDQIETFMLAVARGGGLRALAAMPAVRPLGENGIIWR
                     PLLPFSRQDIWDYARKHGLPNIEDESNTDTAYLRNRFRHRILPELSAQIPHFGRHVLN
                     NVRALQEDLALLEEVVVQDCRWVCGAGYFDTARWLTFSPRRKTHILRNFLKENGIPVP
                     NQNALADIARVLTEAKTGRWNLQGFELHHYAGRLFVFASEQLAKPAFLKDGTISGNLK
                     KILTEHRFVLKRHPFGLPEAMLEQDGILRTVAASDTLAVGGIHKNVKKILQGKRVLPF
                     LRPIWPLVADSGNRPLALANCCADFQISVSDGILPVHPDFPILF"
     gene            complement(21032..21991)
                     /locus_tag="WNA25_000133"
     CDS             complement(21032..21991)
                     /locus_tag="WNA25_000133"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HFA9823005.1"
                     /translation="MKPVFLDFEQPIAELTNKIDELRFVQDESAVDISDEIHRLQKKS
                     NDLTKSIFSKLTPAQISQVSRHPQRPYTLDYIDALFTDFEELHGDRHFADDHAIVGGL
                     ARFNGQSVVVVGHQKGRDTKEKIRRNFGMPRPEGYRKALRLMKTAEKFGLPVMTFIDT
                     PGAYPGIGAEERGQSEAIGKNLYELTRLRVPVLCTVIGEGGSGGALAVAVGDYVNMLQ
                     YSTYSVISPEGCASILWKTAEKAADAAQALGITADRLQKLDLVDTVIKEPLGGAHRDF
                     GQTMKNVKAVLEKQLHEAQSIPLADLLSRRFDRIMAYGKFSEQ"
     gene            22203..22604
                     /locus_tag="WNA25_000134"
     CDS             22203..22604
                     /locus_tag="WNA25_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFA9823006.1"
                     /translation="MKDKHDSSAMRLDKWLWAARFFKTRSLAQKHIELGRVQVNGSKV
                     KNSKTIDIGDIIDLTLNSLPHKIKVKGLNHQRRPAPEARLLYEEDAKTAALREECKQL
                     DQFSRITSAHPDGRPTKRDRRQLDRLKKGDW"
     gene            complement(22672..22869)
                     /gene="iscX"
                     /locus_tag="WNA25_000135"
     CDS             complement(22672..22869)
                     /gene="iscX"
                     /locus_tag="WNA25_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFA9823007.1"
                     /translation="MKWTDTRRIAEELYDLHGEAIDPKTVRFTQLRDLIMALPEFDDD
                     PARCGERILEAVQQAWIDEAE"
     gene            23082..23622
                     /locus_tag="WNA25_000136"
                     /pseudo
     CDS             23082..23622
                     /locus_tag="WNA25_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628869.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(23682..24023)
                     /gene="fdx"
                     /locus_tag="WNA25_000137"
     CDS             complement(23682..24023)
                     /gene="fdx"
                     /locus_tag="WNA25_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFA9823008.1"
                     /translation="MPKITVLPHATLCPEGAVIDNAPEGKTVLDVLLDHDIEVDHACE
                     KSCACTTCHVIIRKGFDSLEEPAELEEDLLDQAWGLEADSRLSCQAVVAGEDLIVEIP
                     KYTINHAREEH"
     gene            complement(24079..24840)
                     /locus_tag="WNA25_000138"
     CDS             complement(24079..24840)
                     /locus_tag="WNA25_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2314 domain-containing protein"
                     /protein_id="HFA9823009.1"
                     /translation="MMGDSVIYYVEQADEPVNRAGERARKTFKYFWRELFWERRRIIS
                     ALDFAMVKVPFFQDGEDGEICEHMWIDDIYFDGLYIYGVLNNEPGGLTNVEQGESVCV
                     PVGDISDWMFVCNGIPYGGFTVQAMRGQMTEEERTEHDTAWGIDFGDPGQVLPVYEEK
                     EHPENLEEHPMCRNCIDDFRQQLSQNPDFLHEQDEDGYTPLHHEAMAGNALMVQAMLE
                     YGANPASKTSEGYTALDFARLTGWQNVADLLEPRH"
     gene            complement(24876..25412)
                     /locus_tag="WNA25_000139"
     CDS             complement(24876..25412)
                     /locus_tag="WNA25_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFA9823010.1"
                     /translation="MRTLPGWNASSPSKPTITKKKNLLHEIFSKSRIGDTELFAVDEN
                     LVKRLFLSLRGEIVFPKNETAESEFEKSVHERRQEGNAGSGRKQLLDLVRRGHREYPY
                     ALPRLLADAASYKPKKSKIRLFKEAYFGKSGTRLTDEIADGIHIYTCFSRADLEKAYS
                     EYLELFKSESDAGGRKPR"
     gene            complement(25710..27572)
                     /gene="hscA"
                     /locus_tag="WNA25_000140"
     CDS             complement(25710..27572)
                     /gene="hscA"
                     /locus_tag="WNA25_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFA9823011.1"
                     /translation="MALLQISEPGMSAAPHRHRLAAGIDLGTTNSLVATVRSGSAACL
                     PDADGRVTLPSVVRYLENGGIEVGKTALSAQKTDPLNTVSSAKRLIGRTLADLHQNTH
                     YLPYRFGDNQRFIELHTRQGVKTPVEVSAEILKTLKLRAEETLGGDLVGVVITVPAYF
                     DDAQRQATKDAARLAGLNVLRLLNEPTAAAIAYGLDNASEGTFVVYDLGGGTFDVSVL
                     QLTKGLFEVKATGGNSALGGDDFDHRLFCYLLEQNRLSQLNEQDSQLLLSLVRAAKEQ
                     LTTQTEARIQATFSDGMAIDTSISRAEFHNLTQHLVMKTFEPVKQALKDAGVGKNEVK
                     GVVMVGGSTRMPHVQQAVATFFGQTPLNNLNPDEVVALGAAIQANVLAGNKADGEWLL
                     LDVTPLSLGLETYGGLAEKIIPRNSTIPTARAQDFTTFKDGQTAMTIHVVQGERELVS
                     DCRSLAKFTLRGIPPMAAGAARIRVTFQIDADGLLSVSAQEQSTGVQAQIEVKPSYGL
                     DDDTITQMLKDSMGNAAEDMAARARAEAVVEAESLTDAVNAALELDSDLLDAEEFAQI
                     QRDIADLQGRLKDGKAEDIRAAVAKLSRSTDNFAAKRMNRNIQRALTGQSVDNI"
     gene            27743..28615
                     /gene="hpnD"
                     /locus_tag="WNA25_000141"
     CDS             27743..28615
                     /gene="hpnD"
                     /locus_tag="WNA25_000141"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HFA9823012.1"
                     /translation="MKGLDYCRQKAEESRSSFLSGFRFLTQEKQDAVTVLYVFCRELD
                     DVVDECSNPDVAQATLNWWRGDLDNAFGGAMPEHPVNQALRQVKETFKLPKYELEALI
                     DGMQMDLVQARYGSFEELKLYCRRVAGVVGCLIARILGFSDGKTLEYADKMGLALQLT
                     NIIRDVGEDARRGRIYLPMEEMQRFDVPASVILQCSPTGNFAELMAFQIKRARETYRE
                     AVSLLPDADKKAQKVGLVMAAVYYALLNEIDRDGAQNVLKYKIALPSPRKKRIALKTW
                     LFGFKPRPGTPERA"
     gene            28690..30003
                     /locus_tag="WNA25_000142"
     CDS             28690..30003
                     /locus_tag="WNA25_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFA9823013.1"
                     /translation="MMNTPHPRPKIAVIGAGWAGLSAAVTLARHADVTLFEAGRQAGG
                     RARTLAGNTDGFGFLDNGQHILLGAYRGVLRLMKTIGSDPRAAFLRVPLHWHMHGGLQ
                     FRALPLPAPLHILGGVLLARRVPSAFKAKLLADMSDLQKSARLGQPDTTVAQWLKQRN
                     VPRAAVMQFWQPLVWGALNTPLETASLRVLCNVLSDGVLTKKSGSDYLLPKQDLGAIV
                     AEPALAELQRLGADIRLETRVCRLNTLPDGKVLVNGEAFDAAILATAPYHAAALLPEG
                     TPEHVQTAYQNLRYHAITTVYLRYAEPVRLPAPLTGIADGTAQWLLCRGRLGLPENEV
                     SAVISVSDRVGAFANRAWADKAHADLRRILPHLGEPEAVRVITEKRATTATDAPPPDL
                     SWLHRHRIFPAGDYLHPHYPATLEAAVQSGFASAEACLQSLNDAV"
     gene            30085..30804
                     /locus_tag="WNA25_000143"
     CDS             30085..30804
                     /locus_tag="WNA25_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFA9823014.1"
                     /translation="MPTLTDKTILVTGASQGLGEQVAKAYAAEGATVILVARHQKKLE
                     KAYDAIVEAGHPEPFAIRFDLMSAEEKEFERFAATIAEATQGKLDGIVHCAGYFYALS
                     PLDFQTVAEWVNQYRINTVAPMGLTRALFPLLKQSPDASVIFVGESHGETPKAYWGGF
                     GASKAALNYLCKVAADEWERFGNLRANVLVPGPINSPQRIKSHPGEARSERKSYGDVL
                     PAFVWWASAESKGRSGEIVYL"
     gene            30970..31641
                     /locus_tag="WNA25_000144"
     CDS             30970..31641
                     /locus_tag="WNA25_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HFA9823015.1"
                     /translation="MKTVSAAIAFAAAAVSLTGCATESSRSLEVAKVASCNTQYHGVR
                     TPISVGTFDNRSSFQKGIFSDSEDRLGSQAKTILVTHLQQTNRFNVLNRTNLSALKQE
                     SGISGKAQNLKGADYIVTGDVTEFGRRDVGDHQLFGILGRGKSQIAYAKVALNIVNVN
                     TSEIVYSTQGAGEYALSNREIIGFGGTSGYDATLNGKVLDLAIREAVDNLVQAVDNGA
                     WQSNR"
     gene            31651..32017
                     /locus_tag="WNA25_000145"
                     /pseudo
     CDS             31651..32017
                     /locus_tag="WNA25_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234874.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4810 domain-containing protein"
     gene            32014..32661
                     /locus_tag="WNA25_000146"
     CDS             32014..32661
                     /locus_tag="WNA25_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 domain-containing protein"
                     /protein_id="HFA9823016.1"
                     /translation="MKPLILGLAAVLALSACQVRKAPDLDYTSFKESKPASILVVPPL
                     NESPDVNGTWGMLASTAAPISEAGYYVFPAAVVEETFKENGLTNAADIHAVRPEKLHQ
                     IFGNDAVLYITVTEYGTSYQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSN
                     SGLLGALVGAVVNQIANSLTDRGYQVSKTAAYNLLSPYSRNGILKGPRFVEEQPK"
     gene            33054..34235
                     /locus_tag="WNA25_000147"
                     /pseudo
     CDS             33054..34235
                     /locus_tag="WNA25_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246189.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            34272..34820
                     /locus_tag="WNA25_000148"
                     /pseudo
     CDS             34272..34820
                     /locus_tag="WNA25_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225253.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            34924..35592
                     /gene="hda"
                     /locus_tag="WNA25_000149"
     CDS             34924..35592
                     /gene="hda"
                     /locus_tag="WNA25_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HFA9823017.1"
                     /translation="MNQLIFDFAAHDYPSFDKFLGTENAELVYVLQHKHDPFIYVWGE
                     EGAGKSHLLQAWVAQALEAGKNAAYIDAASMPLTDAAFEAEYLAVDQVEKLGNEEQAL
                     LFSIFNRFRNSGKGFLLLGSEYTPQQLVIREDLRTRMAYCLVYEVKPLTDQEKIDALA
                     NMAAARQVTVDSEIFEYLLKHWRRDMDSLMMMLDTLDNYAVTMGKRITLPLLRQLLKQ
                     QETQ"
     gene            35589..36257
                     /locus_tag="WNA25_000150"
     CDS             35589..36257
                     /locus_tag="WNA25_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFA9823018.1"
                     /translation="MKNLAIFDLDNTLINTDSDHAWPQYLIKKGLVDAAETEAQNEKF
                     YRDYQNGCLDIDAFLKLHLAPLARYSKEELAEFHREFMAEYIIPHISPMQRMLVQSHQ
                     MAGDETLVISSTNEFIITPVCRLFGIANMIGTQLETGPDGRYTGNYIGTPSLKEGKIT
                     RLNQWLAERGETLESYGKTYFYSDSKNDLPLLRLVSEPVAVNPDAELEKEAKEKGWPV
                     LNFK"
     gene            complement(36848..37744)
                     /gene="argB"
                     /locus_tag="WNA25_000151"
     CDS             complement(36848..37744)
                     /gene="argB"
                     /locus_tag="WNA25_000151"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFA9823019.1"
                     /translation="MEFENIISAADKARILAEALPYIRRFSGSVAVIKYGGNAMTEPA
                     LKEGFARDVVLLKLVGIHPVIVHGGGPQINAMLEKVGKKGEFVQGMRVTDKETMDIVE
                     MVLGGHVNKEIVSMINTYGGHAVGVSGRDDHFIKAKKLLVDTPEQNSVDIGQVGTVES
                     IDTGLVKGLIERGCIPVVAPVGVGEKGEAFNINADLVAGKLAEELNAEKLLMMTNIAG
                     VMDKTGNLLTKLTPKRIDGLIADGTLYGGMLPKIASAVEAAANGVKATHIIDGRLPNA
                     LLLEIFTDAGIGSMILGRGEDA"
     gene            37914..39044
                     /locus_tag="WNA25_000152"
     CDS             37914..39044
                     /locus_tag="WNA25_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase family protein"
                     /protein_id="HFA9823020.1"
                     /translation="MIKISTRFDAGSVVVKDLTDPSNIRLALRPDNASDFAQWFYFRL
                     QGAAYQNCIMHFENAAEAAYPKGWEGYQACASYDRRNWFRVPTSYENGVLTVNHTPLS
                     NSVYYAYFEPYSEEQHLNLLGDAQGSGLCRIDDLGSTVQGRDINLLTIGNQVESDMKI
                     WITARQHPGETMAEWFVEGLLGRLLDSQDPTARTLLDRATFYIVPNMNPDGSALGNLR
                     TNAAGANLNREWENPTLEKSPEVFFVRGKMLETGVDLFLDIHGDEGLPFVFVAGTEGV
                     PNYNPRISALEAQFKTALLNASPDFQDEYGYEKDAPGKANMTLATNWVGNRFNCLAYT
                     LEMPFKDNANLPDDDFGWNGQRSLRLGEAVLSAILNVAGDLR"
     gene            39118..39969
                     /locus_tag="WNA25_000153"
     CDS             39118..39969
                     /locus_tag="WNA25_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFA9823021.1"
                     /translation="MIIHHQFDPVLISIGPLAVRWYALSYILGFILFTFLGRRRIAQG
                     LSVFTKESLDDFLTWGILGVILGGRLGYVLFYKFSDYLAHPLDIFKVWEGGMSFHGGF
                     LGVVIAIWLFSRKHGIGFLKLMDTVAPLVPLGLASGRIGNFINGELWGRITDINAFWA
                     MGFPQAHYEDAEAAAHNPLWAEWLQQYGMLPRHPSQLYQFALEGICLFAVVWLFSKKP
                     RPTGQTAALFLGGYGVFRFIAEFARQPDDYLGLLTLGLSMGQWLSVPMIVLGIVGFVR
                     FGMKKQH"
     gene            complement(40054..40716)
                     /locus_tag="WNA25_000154"
     CDS             complement(40054..40716)
                     /locus_tag="WNA25_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecA family protein"
                     /protein_id="HFA9823022.1"
                     /translation="MDSRKFTEASKRRLGELLDAKSEQGNTMRCDEVQGFMTALLSGP
                     DKLAPLDWLPEVLGDESQFTAAERSEIERLVLAMAMETTAAMSDKKLPDLWLYDDGEG
                     GSDFYTWCNAYLYGLDIVPTDWFEAADDEAFEELFYPVMALGGIYDEEENGAIRLQFT
                     EGELAELASELPYALADIYRYWQAVINKPQTVRREGEKTGRNDPCPCGSGRKYKACCG
                     KN"
     gene            40750..40856
                     /locus_tag="WNA25_000155"
                     /pseudo
     CDS             40750..40856
                     /locus_tag="WNA25_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(40915..42708)
                     /locus_tag="WNA25_000156"
     CDS             complement(40915..42708)
                     /locus_tag="WNA25_000156"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFA9823023.1"
                     /translation="MQLDIDRLVAYFGGVNALAEALKRHDPENAATTAAIYKWRTRGS
                     LPLAQLQKLTALAESQGRPLDLNAFLQKNESLERTEMTQANRVIIFDTTLRDGEQSPG
                     AAMTKEEKIRVARQLEKLGADIIEAGFAAASPGDFEAVNAIAKTITKSTVCSLSRAIE
                     RDIRQAGKAVAPAPKKRIHTFIATSPIHMEYKLKMKPKQVIEAAVKAVKIAREYTDDV
                     EFSCEDALRSQIDFLAEICGAVIEAGATTINIPDTVGYSIPYKTEEFFRELIAKTPNG
                     GKVVWSAHCHNDLGLAVANSLAALKGGARQVECTVNGLGERAGNASVEEIVMALKVRH
                     DLFGLETGIDTTQIVPSSKLVSTITGYPVQPNKAIVGANAFSHESGIHQDGVLKHRET
                     YEIMSAESVGWSANRLSLGKLSGRNAFKTKLADLGIELESEEALNAAFARFKELADKK
                     REIFDEDLHALVSDEMGNMNAESYKFISQKISTETGEEPRADIVFGIKGEEKRASATG
                     SGPVDAIFKAIESVAQSGATLQIYSVNAVTQGTESQGETSVRLARGNRVANGQGADTD
                     ILAATAKAYLSALSKLEFSAAKPKAQGSGTI"
     gene            42954..44063
                     /gene="proB"
                     /locus_tag="WNA25_000157"
     CDS             42954..44063
                     /gene="proB"
                     /locus_tag="WNA25_000157"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFA9823024.1"
                     /translation="MKYKRIVFKVGTSSITHSDGSLSRGKIQTITRQLAALHHAGHEL
                     VLVSSGAVAAGFGALGFKKRPVKIADKQASAAVGQGLLMEEYTANLSSDGIVSAQILL
                     SRADFADKRRYQNAGGALSVLLQRRAIPIINENDTVSVEELKIGDNDTLSAQVAAMIQ
                     ADLLVLLTDIDGLYTGNPNSNPDAVRLDKIEHINHEIIEMAGGSGSANGTGGMLTKIK
                     AATIAAESGVPVYICSSLKPDSLAEAAEHQADGSFFVPRAKGLRTQKQWLAFYSESRG
                     SVYVDEGAEHALSEQGKSLLMSGIAGIEGHFSRMDTVTVYSKATKQPLGKGRVLFGSA
                     AAEDLLKSRKAKGVFIHRDDWISITPEIRLLLTEF"
     gene            44098..45339
                     /locus_tag="WNA25_000158"
     CDS             44098..45339
                     /locus_tag="WNA25_000158"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003683179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFA9823025.1"
                     /translation="MPLPKRQKKSVNTADAEEKNRALLAMADSLEAAAEDILAANRLD
                     LKAAAGKIPDSMTDRLLLDGKRICAMADGIRAVAALPDPVGEILETSTLPNGLEIVKK
                     RVAMGVIGIIYESRPNVTSDAAALALKSGSAVVLRSGKDAFQSARAIVAALKTGLAQT
                     RIDPEAVQLIEDTGREGSYEMMRAKDYLDLLIPRGGAGLIRAVVENAVVPVIETGTGI
                     VHIYIDKDADWDKALRIVYNAKTGRPSVCNSMEVLLVHEGIAADFLPKLERLLVRGRI
                     EAGLPPVRFRLDPQAARYIGGEAAGADDFDTEFLDYILAVKTVASVEEAVGHIEARGT
                     HHSDGIVTENRHAADYFTTHIDSAAVYINASTRFTDGGEFGLGCEMGISTQKLHARGP
                     MGLKELTSYKYIVQGTGQVRE"
     gene            complement(45515..48559)
                     /locus_tag="WNA25_000159"
     CDS             complement(45515..48559)
                     /locus_tag="WNA25_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFA9823026.1"
                     /translation="MFWIVLIVILLLALAGLFFVRAQSEREWMREVSAWQEKKGEKQA
                     ELPEIKDGMPDFPEFSLMLFHAVKTAVYWLFVGAVRFCRNYLAHESEPDRPVPPASAN
                     RADVPTASDGYSDSGNGTEEAETEAAEAAEEEAADTEDIATAVIDNRRIPFDRSIAEG
                     LMQSESKTSPVRPVFKEITLEEATRALSSAALRETKKRYIDAFEKNGTAVPKVRVSDT
                     PMEGLQIIGLDDPVLQRTYSRMFDADKEAFSESADYGFEPYFEKQHPSAFSAVKAENA
                     RNAPFRRHAGQEKGQAEAKSPDVSQGQSVSDGTAVRDARRRVSVNLKEPNKATVSAEA
                     RISRLIPESRTVVGKRDVEMPSETENVFTETVSSVGYGGPVYDEAADIHIEEPAAPDA
                     WVVEPPEVPEVAVPEIDILPPPPVSEIYNRTYEPPAGFEQAQRSRIAETDHLAADVLN
                     GGWQEETAAIADDGSEGAAERSSGQYLSETEAFGHDSQAVCPFEDVPSERPSCRVSDT
                     EADEGAFQSEETGAVSEHLPTTDLLLPPLFNPEATQTEEELLENSITIEEKLAEFKVK
                     VKVVDSYSGPVITRYEIEPDVGVRGNSVLNLEKDLARSLGVASIRVVETIPGKTCMGL
                     ELPNPKRQMIRLSEIFNSPEFAESKSKLTLALGQDITGQPVVTDLGKAPHLLVAGTTG
                     SGKSVGVNAMILSMLFKAAPEDVRMIMIDPKMLELSIYEGITHLLAPVVTDMKLAANA
                     LNWCVNEMEKRYRLMSFMGVRNLAGFNQKIAEAAARGEKIGNPFSLTPDDPEPLEKLP
                     FIVVVVDEFADLMMTAGKKIEELIARLAQKARAAGIHLILATQRPSVDVITGLIKANI
                     PTRIAFQVSSKIDSRTILDQMGAENLLGQGDMLFLPPGTAYPQRVHGAFASDEEVHRV
                     VEYLKQFGEPDYVDDILSGGGSEELPGIGRSGDGETDPMYDEAVSVVLKTRKASISGV
                     QRALRIGYNRAARLIDQMEAEGIVSAPEHNGNRTILVPLDNA"
     gene            48724..49404
                     /locus_tag="WNA25_000160"
     CDS             48724..49404
                     /locus_tag="WNA25_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823027.1"
                     /translation="MIFQTVWFSDVVLSVSWIVLILILAASAPSAFRSLARYRSALPL
                     CTVIFSAAWCLNASVGGGQLAQMNYHLLAVNLVTLMVDTSAALWLAALLMLPYCLLFA
                     GSAGAYPPNALVLILPALVVNRLSRMLVNRLPPNIFIFIFVNGFLASAAGILLTGLVL
                     IGILDAANAFPSEILWTTALPVFILLAWAEAFLSGISTAIFVALKPHWINTFDDNRYL
                     KSERGIWL"
     gene            49433..49792
                     /locus_tag="WNA25_000161"
     CDS             49433..49792
                     /locus_tag="WNA25_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HFA9823028.1"
                     /translation="MLSNILPLSIGAIFGTTARWLLNLAVPASLSPATGNLFANWTGA
                     LLIGIFAETVSHPQWKLLLITGFFGSLTTLSGFSLETVTLLQSNRPASALANIFLHTA
                     GSLLLTWLGLKIGTAVK"
     gene            49870..49976
                     /locus_tag="WNA25_000162"
                     /pseudo
     CDS             49870..49976
                     /locus_tag="WNA25_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(50397..50933)
                     /locus_tag="WNA25_000163"
     CDS             complement(50397..50933)
                     /locus_tag="WNA25_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFA9823029.1"
                     /translation="MDLREVKLGGETIYEGGFVSISRDKVRLPNGNEGQRIVIRHPGA
                     ACVLAVTDDEKVVLVRQWRYAANQATLELPAGKLDVAGEDMAACALRELAEETPYVAD
                     SVRLLYSFYTAVGFCNEKMYLFEAEGVRLGSTLSNDEDEITETVLMSKDEVRQALAND
                     EIKDGKTLIGLQYWLMKD"
     gene            complement(50967..51323)
                     /gene="folB"
                     /locus_tag="WNA25_000164"
     CDS             complement(50967..51323)
                     /gene="folB"
                     /locus_tag="WNA25_000164"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HFA9823030.1"
                     /translation="MDKIFLHGMKADTLIGVYGWERERLQTLIVDLDIGVPEKAGSDD
                     DIANTVHYAEVCETLRRHLKEQDFLLLEALAEYIADLVLGYFGAVWVHVKIVKPGILE
                     GVREVGVEIERGKRED"
     gene            51379..51981
                     /gene="plsY"
                     /locus_tag="WNA25_000165"
     CDS             51379..51981
                     /gene="plsY"
                     /locus_tag="WNA25_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFA9823031.1"
                     /translation="MFNIPAVAVSYLIGSLSFAVIVSKYYGMDDPRTYGSGNPGATNV
                     LRSGKKKAAALTLLGDAAKGLVAVLLARVLQEPLGLSDSAIAAVALAALVGHMWPVFF
                     GFKGGKGVATALGVLLALSPATALVCALIWLVMAFGFKVSSLAALVATTAAPLAALFF
                     MPHTSWIFATLAIAILVLLRHKSNILNLIKGKESKIGEKR"
     gene            52073..52918
                     /locus_tag="WNA25_000166"
     CDS             52073..52918
                     /locus_tag="WNA25_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HFA9823032.1"
                     /translation="MLSYRHAFHAGNHADMLKHFTLFLVLQYFNRKDKPYWYIDTHGG
                     AGLYNLEGSEAQKVGEYRQGIALLRQAQNLPAELSDFAAHIQKILPSPELYCGSPWLA
                     QSLIRIGDKLRLFELHPADFVHLQNNMGEAGLGKRGQVLREDGYKGLISLLPPPPRRA
                     VILIDPPYEEKQDYRRVTETLKAALKRFESGCYLIWYPCLSREESRKLPEELKKLLPD
                     NYLYAELHVHAPKTDGFGMHGSGMFVINPPYLLAEQLAANLPALTRLLAQDEGARYLL
                     DNKIR"
     gene            complement(53027..53359)
                     /locus_tag="WNA25_000167"
     CDS             complement(53027..53359)
                     /locus_tag="WNA25_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823033.1"
                     /translation="MKNLKNISVVAVCAVLLAACASENSVANYAIGDDSAVIKAGRNR
                     AEARISRAELAQHRRQRKNVSEELALEREKRANKHDAIRQGMGTAAGGLMLLNGVVGT
                     VGVMKSVF"
     gene            complement(53409..53657)
                     /locus_tag="WNA25_000168"
     CDS             complement(53409..53657)
                     /locus_tag="WNA25_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823034.1"
                     /translation="MKMKKLILLSVAAMLVTACTYADRRFVTQESAAEIQAKSRAIQI
                     SERAERAEYRKERREEMMDAARAIKKANENSPNIYFIR"
     gene            complement(53714..53875)
                     /locus_tag="WNA25_000169"
     CDS             complement(53714..53875)
                     /locus_tag="WNA25_000169"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823035.1"
                     /translation="MPSERRQIAAGRSFGAFRRISRGIVVEKNGAGQVGTRQADREVQ
                     DAKFIFKKY"
     gene            53900..54874
                     /locus_tag="WNA25_000170"
     CDS             53900..54874
                     /locus_tag="WNA25_000170"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFA9823036.1"
                     /translation="MDTLTRFLPEHLQQNQLPEALGGVLLSVVSACTEINAKVRLGAL
                     AGVLGMAGTGNIQGEDQKKLDVIANNIMIDTLKANPAVAGLASEEEDTFVSAGENGRY
                     LVLFDPLDGSSNIDVNISVGTIFSILAKPEGALATESFLQTGRQQLAAGYVLYGPQTQ
                     LVFTFGHGVYVFTLNAENEFVLTKENPKVPESTKEFAINMSNRRHWLPPVQQYVDELL
                     AGETGTRGKNYNMRWVASMVAEIHRILMRGGVFMYLQDKRDPSKPGKLRLMYEANPMA
                     LILEQAGASASNAYQAMLDIQPESLHQRVAVIMGSSEEVDYLNRLHSK"
     gene            complement(55033..55251)
                     /locus_tag="WNA25_000171"
     CDS             complement(55033..55251)
                     /locus_tag="WNA25_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFA9823037.1"
                     /translation="MFPEYRDLISKLKQENSRFARLFDEHNELDDKITGLANNPVTSG
                     AETIDELKKAKLKLKDELYAILQKAAGK"
     gene            55445..55708
                     /locus_tag="WNA25_000172"
     CDS             55445..55708
                     /locus_tag="WNA25_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HFA9823038.1"
                     /translation="MKGTPQFPQCGFSARAVEVPTQIGRPFAFVNILENPEIRSTLPL
                     IANWPTFPRLWVNGELIGGSDIILQMYQSGELKPLIEEHSPKV"
     gene            55876..57644
                     /gene="ggt"
                     /locus_tag="WNA25_000173"
                     /pseudo
     CDS             55876..57644
                     /gene="ggt"
                     /locus_tag="WNA25_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217281.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            57708..57872
                     /locus_tag="WNA25_000174"
     CDS             57708..57872
                     /locus_tag="WNA25_000174"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823039.1"
                     /translation="MSNQTKIQAIKQASEQILTICETPNTALQAIHLILRHGGAGRIV
                     LASGVSAGYG"
     gene            57817..58059
                     /locus_tag="WNA25_000175"
     CDS             57817..58059
                     /locus_tag="WNA25_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823040.1"
                     /translation="MAGQEELSWQVVYQRVMADKDVVGAGYLIDFAQTAENLPFDVLP
                     LISLVLNKGDETLKTGMLNKLPDNAKENLRIMGYLP"
     gene            58126..59376
                     /locus_tag="WNA25_000176"
     CDS             58126..59376
                     /locus_tag="WNA25_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFA9823041.1"
                     /translation="MFSKSVTLAQYDPDLAAAIAQEDRRQQDHVELIASENYVSCAVM
                     EAQGSQLTNKYAEGYPAKRYYGGCEYVDIVEQLAIDRVKELFGAAYANVQPHSGSQAN
                     QAVYASVLKPGDTILGMSLAHGGHLTHGASVNISGKLYNAVTYGLDENEVLDYAEVER
                     LALEHKPKMIVAGASAYALQIDWAKFREIADKVGAYLFVDMAHYAGLVAGGEYPNPVP
                     FCDFVTTTTHKTLRGPRGGVILCRDNTHEKALNSSIFPSLQGGPLMHVIAAKAVAFKE
                     ALQPEFKQYAKQVKINAAVMAEELVKRGLRIVSGRTESHVFLVDLQPMKITGKAAEAA
                     LGKAHITVNKNAIPNDPEKPFVTSGIRIGSAAMTTRGFNETDARVLSNLVADVLANPE
                     DEANLAKVRGQVTALCDKYPVYGN"
     gene            complement(59449..59838)
                     /locus_tag="WNA25_000177"
     CDS             complement(59449..59838)
                     /locus_tag="WNA25_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFA9823042.1"
                     /translation="MEVHDKIRTLREVNQWTQEEMAEKLEMSVNGYSKIERGKSGINL
                     DKLRQIAQIFNIDVVELLAEQNRSFFFSIGDNTNNHHNIIGSDEMLVFENEKLRSLLD
                     AKDELIRQKDSEIAVLKKLVILLEEKK"
     gene            60778..61569
                     /locus_tag="WNA25_000178"
     CDS             60778..61569
                     /locus_tag="WNA25_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HFA9823043.1"
                     /translation="MKKALLALTIAAISGTAMAQLPDFLGKGEYTVRTDISKQTLKNA
                     DLKEKHKVQKNIGFRADMPFDDIHHGMRFEVSHSRDKKDMYVVTESTTKPFGKDVKEK
                     RTDVYAGYTYTQPLSEATKLRAGLGLGYEKYKDAVANEKGTVSTEREAFYTKAHADLT
                     SDLGGGWYLNPWAEVKVDLDAKLKHNATVAGASADINAKTRGWGVGVGANIGKQITDT
                     VGIEAGPFYKHRHFKASGSFVLDGGNIRVDPTKINEYGVRVGVKF"
     gene            61720..62364
                     /locus_tag="WNA25_000179"
     CDS             61720..62364
                     /locus_tag="WNA25_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFA9823044.1"
                     /translation="MLASVIDFILHIDQHLLSLSAQYGVWIYAILFLIVFCETGLIVT
                     PFLPGDSLLFAAGGIAALGGMDIHLMVALLSLAAILGDALNFTVGKYFGGRLFANPDS
                     KIFRCEYLGKTRRFYEKHGGKTIIIARFVPIVRTFAPFVAGMGKMHYAKFIRYNIIGG
                     LLWVILFSYAGYFFANFPVVKNNLGLVMGGIIIVSVLPGIIEIARAKLAAKSER"
     gene            62383..64293
                     /locus_tag="WNA25_000180"
     CDS             62383..64293
                     /locus_tag="WNA25_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFA9823045.1"
                     /translation="MNILSVENASFAVGHVALLDKTSFQLDSGEKVGLIGRNGAGKSS
                     FLKILTGVQKLDDGQIIVQNNLKIVYVPQESFFDKDATVFDTVAEGLGEICDLLRRYH
                     HVSHELENGSSELLLKELNELQLEIEAKDGWKLDAAVKQTLGELGLPENEKIGNLSGG
                     QKKRVALAQAWVQKPDVLLLDEPTNHLDIDAIIWLENLLKAFEGSLVVITHDRRFLDN
                     IATRIVELDRGILRSYPGSFSKYSEKKAQELAVEAEHNRLFDKFHAQEEAWIRKGIEA
                     RRTRNEGRVRRLEELRRQRAERRNVQGQVNFKLDSGKKSGKIIAELEHASFAYDDKVI
                     MDKFSAILQRGDKIGLIGPNGIGKTTFLKLILGELQPTYGRIRIGSKQEVAYFDQFRS
                     ALNENDTVFYTLGQGNDYVEVGGKKKHVMSYLEDFLFPPARAQSPVSSLSGGERNRLL
                     LAKLFTRPANILVLDEPTNDLDIDTQELLEDLLRDYQGTVFLVSHDRMFLDNVITQSI
                     VFEGQGRLKEYIGGYQDYIDAKSRENKIQTASAPKASDVEPAKEKPKANRTVKLSYKE
                     QRELDALPDEIAALETEQAEINAQLSDPGIFKDYEKAGALQNRAEEIEMLLLEKLERW
                     ELLETKQNGNAV"
     gene            64315..64681
                     /locus_tag="WNA25_000181"
                     /pseudo
     CDS             64315..64681
                     /locus_tag="WNA25_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213667.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            64793..66268
                     /locus_tag="WNA25_000182"
     CDS             64793..66268
                     /locus_tag="WNA25_000182"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFA9823046.1"
                     /translation="MISKQEYQAQAAQGYNRIPLVQELLADLDTPLSLYLKLANRPYT
                     YLLESVVGGERFGRYSFIGLPCSHYLKAGGKHVDVYQNGEIVEQYDGNPLPFIEAFHN
                     RFKTPEIPSLPRFTGGLVGYFGYETVYNFEHFAHRLKNTAKANPLGTPDILLMLSQEL
                     AVIDNLSGKIHLIVYADPSQPDSYERARERLEDIRTQLRQSCAIPLSLGSKQTQAVSE
                     FGEEPFKACVDKIKDYIFAGDCMQVVPSQRMSMEFTDNPLALYRALRTLNPSPYLFYY
                     DFGDFHIVGSSPEILVRRERDDVIVRPIAGTRLRGKTPAEDLANEQDLLSDAKEIAEH
                     VMLIDLGRNDVGRISKTGEVKVTDKMVIEKYSHVMHIVSNVEGCLKEGVTNMDILAAT
                     FPAGTLSGAPKVRAMEIIEEIEPEKRGIYGGAVGVWGFNNDMDLAIAIRTAVIKNNTL
                     FIQSGAGVVADSDPTSEWQETQNKARAVVRAAQMVQEGLDK"
     gene            66352..67290
                     /locus_tag="WNA25_000183"
     CDS             66352..67290
                     /locus_tag="WNA25_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFA9823047.1"
                     /translation="MQFTSLEICAGAGGQALGLERAGFSHVALIEIEPSACQTLRLNR
                     PDWNVIEGDVRLFQGEGYDGIDLLAGGVPCPPFSKAGKQLGKDDERDLFPEAIRLAKE
                     TDPKAIMLENVRGLLDPKFENYRNHITEQFAKLGYLGQWKLLYAADYGVSQLRPRVLF
                     VALKNEYTNFFKWPEPNSEQPKTVGELLFDLMSENNWQGAHNWRLKAAQIAPTLVGGS
                     KKHGGADLGPTRSKRAWAELGVDGSGLWDSAPPEDFSGMPRLTVRMTARIQGFPDNWQ
                     FFGKKTPMYRQIGNAFPPPVAEAVGRQIIKALKKEN"
     gene            67291..68151
                     /locus_tag="WNA25_000184"
     CDS             67291..68151
                     /locus_tag="WNA25_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-2 restriction enzyme NgoMIV"
                     /protein_id="HFA9823048.1"
                     /translation="MNPLFTQERRIFHKKLLDGNILATNNRGVVSNADGSNTRSFNIA
                     KGIADLLHSETVSERLPGQTSGNAFEAICSEFVQSAFEKLQHIRPGDWNVKQVGSRNR
                     LEIARYQQYAHLTALAKAAEENPELAAALGSDYTITPDIIVTRNLIADAEINRNEFLV
                     DENIATYASLRAGNGNMPLLHASISCKWTIRSDRAQNARSEGLNLVRNRKGRLPHIVV
                     VTAEPTPSRISSIALGTGEIDCVYHFALYELEQILQSLNYEDALDLFYIMVNGKRLKD
                     ISDLPLDLAV"
     gene            68168..69304
                     /locus_tag="WNA25_000185"
     CDS             68168..69304
                     /locus_tag="WNA25_000185"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003683536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFA9823049.1"
                     /translation="MNTPPILPPAMLGILGGGQLGRMFAVAAKTMGYKVTVLDPDPNA
                     PAAEFADRHLCAPFDDRAALDELAKCAAVTTEFENVNADAMRSLAKHTNVSPSGDCVS
                     IAQNRIQEKARIRKAGLQTAPYQAVCKAEDITEASAQFLPGILKTATLGYDGKGQIRV
                     KTLDELKAAFAEHGGVDCVLEKMVDLRGEISVIVCRLNDENVQTFDPAENIHENGILA
                     YSIVPARLSADVQQQARQTAQRLADELDYVGVLAVEMFVVGDTHELLVNETAPRTHNS
                     GHHTIDACAADQFQQQVRIMCNLPPADTKLLSPCCMANILGDVWQEDGGEPDWLPLQS
                     RPNAHLHLYGKKTAQKGRKMGHFTVLTTDSDTAFQEAKKLHQSL"
     gene            69351..69833
                     /locus_tag="WNA25_000186"
     CDS             69351..69833
                     /locus_tag="WNA25_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFA9823050.1"
                     /translation="MSLLTLLRPATVQDCKDIFKVHLHSVQYTCILSYNEHALKVWEG
                     LLNTESYLPTISDPDKALWVAEYKGNIQGFFQIDCQEAQLDALYVHPLFHNLGLGTAL
                     LRQAETIAHKSGLSFLKLYASLNSVPFYLLNRYESLGSAVLQLDPSIKIKCELMRKHL
                     "
     gene            69986..70987
                     /locus_tag="WNA25_000187"
     CDS             69986..70987
                     /locus_tag="WNA25_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFA9823051.1"
                     /translation="MQPRSRFKPSVRTKCPGQYRIRRKKHYPAQCLIRCDTVFLQRIQ
                     PLIYQNIPIRTPRHIRQHPTIPRRLQQTGIIRSQRPSSRCRNHEPIFRHRPARKKGLV
                     EKGWQQALPDHAAPYTSTMVFLVRKNNPKQIRDWNDLAKDGANIVIAKTSGNGRYAFL
                     GAYGYGLKANNGNEQEAQKLVASILKNTPVFENGGRAAATTFTQRNIGDVLITFENEA
                     NYVSKKLTQGQFEIVYPSYTISAESPVAVVNSVVAKKGTQKTARAYLEYLWSEPAQEL
                     AASLYLRPRNPEVLARHKADFPDLDTFPPEEKFGGWDNIMKTYFADGGVFDRLTAQK"
     gene            71567..72172
                     /locus_tag="WNA25_000188"
     CDS             71567..72172
                     /locus_tag="WNA25_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HFA9823052.1"
                     /translation="MNTDFQKILKQLGKQAKKEAAEKQEAEKNKQKQEYDLDFSQAVG
                     QVSPLKNRQQYYAQSDKTPIKARPKDNRTDEENYFYIGSTYNDPPASFCKNGQGKNDI
                     QRLKNGYYPVVTDVDLHGYTQEEAQKVLNEFIAFTQKRGVCGEIIHGSGLGSKGYKPV
                     LKNMTRNWLMQHPDVLAYVEPREGNDGCVRILLKRKLRQQD"
     gene            72235..72597
                     /gene="ssrA"
                     /locus_tag="WNA25_000189"
     tmRNA           72235..72597
                     /gene="ssrA"
                     /locus_tag="WNA25_000189"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT        17642 a        20648 c        17979 g        16612 t
ORIGIN      
        1 cggaaccggt ttctttgcct ttttccccga ttcggcaacg gcaggcacgc cccttcccct
       61 tactttgccg catcgggctg tttttttctc ggcggtggtt ttcgatttcc ctttccccgc
      121 cgcccctgcc ggtttggcag atgcggataa tttgaccttc cgccgcctgc cgcttcctcc
      181 ggcaattagg acaaagtcga tttttccatc atccaaatcg gcacgcgcga cccggacggc
      241 aaccctgtcc cccatattga aacggatgcc gctgcgttcg ccttcgattg ccatgatttc
      301 ggggcggaag ttgaaataat cttcgcccaa atcgctgata tgtaccagac cgtcgatatg
      361 gatatcgtcc aaagtgacaa atattccaaa atttgccacc ccggagattt tgccttcaaa
      421 tatttcaccg accttatcgc gcatataata agttttcagc cagttttcca catcgcggcc
      481 agcatcgtcg gcacggcgtt cgcaaaacga agtatgcacg cccaaagcct gccagctttt
      541 gtttggcgtg taggttttcc ggttcaatac ggctttgatg gcacggtgga cggtcaggtc
      601 gggatagcgg cggatgggcg aggtaaagtg ggcgtatgct tcataagcca aaccgaaatg
      661 cccttcgcaa tgcggttcgt aaaccgcctg ctgcatggag cgcaacatca tgacttgcag
      721 caattcggca tccggcctgc ctttgaattg ttcggcaagc gcggcatagt ctttcggcga
      781 cgggttgtcg ccgccgccaa gttgaagccc caacagaccg agctgctcgc gcagggtggc
      841 gagtttttcg gacgtggggc ccaaatggtt gcggaacaat gcggtatgct tgtttttcaa
      901 cagaaaatcc gccgcgcaaa cattcgccgc caacatacat tcttcaatca gcttatgggc
      961 atcgttgcgg acaacgggca cgattttttc gattttgccg ttgccgtcga aaagcatttg
     1021 cgtttcgacg ctttcaaact cgaccgcgcc gcgcgccaga cgttttttct gcaaaatctt
     1081 aaacagcttg taaagcgtgt cgatttgggc tttgtgcgga ttcccgatgc cgtctgaaag
     1141 ccatttccaa acttggttgt aggtcaggcg ggcatgagag cgcatcacgg cgggatagaa
     1201 gcggtattct ttgatattgc ccgcataggt aacgaccata tcgcacacca tacacaaacg
     1261 ctcgacatcg ggattgagcg agcagatgcc gttggacagg ttttccggca gcatcggaat
     1321 catacggcgc gggaaataca cgctggtact gcgttcttga gcatctgcat caatcgcatc
     1381 gtcagggcgg acataatggc tgacatccgc aatcgccacg accagacggt aattgcgtcc
     1441 gactttttcg gcaaacaccg cgtcgtcgaa atcgcgcgcc gtttcgccgt ctatcgttac
     1501 caaaggaagg tcgcacaaat cgacgcggcc tttcaaatcg cttttgcgta catggtcggg
     1561 aatttttttc gcggatttgg cacacgcttc actgaatcgg tgcggcaaat gatgcttgcg
     1621 cacggcaatt tcgatttcca tcccgctgtc ggcataatcg cccaaaactt caatgatttt
     1681 tgccactgca ggccggtttt gctcgggata aacctcaatt ttgccgacga taacctgacc
     1741 ggattcgggt ttgaaacgcg ccacgccgtc cggttccaac acgatgcttt ggttcagacg
     1801 cttgtcttcc ggctccaaaa tcgccacgcc cctatccata tagaaacggc cgacaacttt
     1861 gctttgcgcg cgttcgacaa tatccagaac cgtcccttcg cggcggcccc tgcggtcgat
     1921 acccgcagga cggacggtaa cggtgtcgcc gtgcatgaca ccacgcatct ggcgttcgta
     1981 taaaacgaaa tccccttcgt ccatcggcat gagcggcacg gcgaagccga aaccgtcctt
     2041 atgcgcctcg acgcggcatt tgaccaaatc cagcttgtcc gccgcgcaaa ctgcgcctcg
     2101 gcggttgatt aaaacctgac cgtcccgcgc catcgccttc agacggcgtt caaaaaagac
     2161 atactcgtct tccgtaatcg acagctcgcg tgcaagcgat tcgatttttg aaggcacacc
     2221 tttgcgctcc aacaattcga ttatccattc ccgactgggc aaaggatgtt cataacgctg
     2281 tttttcacga cttaaaaacg ggtctttttc ccgtaaattt aaagatttaa tatttttatt
     2341 cattttgact gttgacattc tctttatgaa ctatataata gcgacttctt tgcggcagga
     2401 cactgtaccg caaaacagca aataaagcaa agcccgggtg gcggaattgg tagacgcgcc
     2461 agcttcaggt gctggtatcc tcacgggtgt ggaagttcga gtcttctccc gggcaccaaa
     2521 taacaaatta tcccgattag gataatttcc gccattagag aggtggatga gtggtttaag
     2581 tcgcacgcct ggaaagcgtg tatacgtgaa tagcgtatcg agggttcgaa tcccttcctc
     2641 tctgccaagt acaaatccca aacttttaca gtttgggatt ttttgttact tacgtattga
     2701 ttttattgcg taaaatcagc cacaacctat taccctacgt taccattgat tactatccgt
     2761 taccgttttt ttatggtatg ttttgcggta tgttttatcc tcttcctaag cctgtaagga
     2821 ctcaaaaggc gtatcccttc aaacgatatt gttcgacatg ctgccgggaa accgaacccg
     2881 ataccgaaac gcgacggggc gatatgccgc tggaaaaatg gcggcagttt cttgattgga
     2941 taggaaaaac gccgtctgaa acagcttcaa gaagccgcaa agcccgtccg aataaaaaat
     3001 accgtctgaa atttttcaga cggcatcgcg tatcatttaa acattaatcc agctttttaa
     3061 aatgacggcg gcgttccaat tcgctcaggt aacgcttgcg caggcggatg gattgcggcg
     3121 tgatttccac cagctcgtca tcgtcgataa actcgaccgc gccttccagc gtcagtttga
     3181 tcggcgtggt caggcgcacc gcttcgtcgg taccgctggc acggatattg gtgagttttt
     3241 tgcctttgag cgggttgacc accaaatcgt tgtcgcggct gtggatgccg ataatcatac
     3301 cttcgtagat tttgtcgttg ggcgatacga acatacggcc gcggtcttca agattccaca
     3361 aggcgtaagc aaccgcctcg ccctgctctt gggacaccag tacgccgttg tggcggccgg
     3421 gcatatcggg tttgacgggc gcgtagtcgt cgaacacgtg gctcatcagc ccgacgccgc
     3481 gcgtcagggt catgaattcg ccttggaaac cgatcaagcc gcgcgctgga atatggtatt
     3541 cgaggcgggt gcgtccgttg ccgtcgcttt ccatattggt cagttcgcca cggcggcggc
     3601 cgagttcttc cattaccgcg ccttggttgt cgtcgggtac gtccacagtc aggttttcat
     3661 aaggttcgca tttttgaccg tcgatgtctc ggtacacgac gcgcggcttg ccgacggcga
     3721 gttcgtagcc ttcgcggcgc atattttcca gcaaaatcgt caggtgcagt tcgccgcgcc
     3781 cggatacgcg gaacacgtcg gcatcggcgg tgtcttccac gcgcagggca acgttggtca
     3841 gcaattcttt ttgcaggcgg tcgcggattt ggcggctggt cacgaatttg ccttctgtac
     3901 ctgcgagcgg gctggtgttt accataaagt ccatcgtcag cgtcggttcg tccactctca
     3961 acatcggcag gcctttgggg ttgtctttgt cggtgatggt tacgccgatg ccgatgtctt
     4021 cgataccgga aataatcacg atgtcgccgg cttcggcttc ttcaagcggc acgcgttcca
     4081 agcctttgaa acccaaaagc tggttgatgc ggccttgggc gatttgctgc tcgtggttca
     4141 tcacggcaac ggtttgaccg ggtttgatgc gtccgttcaa gatacgaccg ataccgaggc
     4201 ggccggtgta gttgtcgtag tcgagttggg aaatttgcag ttgcagcggc tcgtccgcgc
     4261 tgccgctcgg tgcaggcgtg tattttagga tggtgtcgaa cagcgggcgc atatcgctgc
     4321 tctcgtcggt ttcttccagc ttggcaaagc cgctcaaacc tgaagcgtaa acaatcggga
     4381 aatccaactg ctcgtcggtc gcacccaagt tgtcgaacaa ctcgaaagtc tggtcgataa
     4441 cccagctcgg acgggcggac ggtttgtcga ttttgttgat gacgacaatc ggtttcagcc
     4501 ccaaagccaa ggcttttttg gtcacgaaac gggtttgcgg catcggacct tcctgtgcgt
     4561 ccaccaacaa gacgacgcaa tccaccatcc ccaaaacgcg ctccacttcg ccgccgaagt
     4621 cggcgtgtcc cggcgtgtcg acgatattga tgtggcagcc ttcgtaatcg atggcggtgt
     4681 ttttggcgag gatggtgatg ccgcgttctt tttcaaggtc gttgctgtcc atcacgcgct
     4741 cgtcaacctg ctggttggcg cggaatgtgc cggattggcg cagcagttgg tcgaccaatg
     4801 tggttttgcc gtggtcgacg tgtgcgatga tggcgatgtt gcggatttgt ttcataatta
     4861 atgtttttca aaaactgtaa gagataactg cgtattatac cacgatttac cggacagtat
     4921 ttggaaaatg aagatgccgt ctgaagccct tgcctttcag acggcatctt cttacatact
     4981 ttcagttatc cagttgcgca gccaccttgc cgccggggcg atttcccctg ccgacagccc
     5041 agccgcaatg cctgccgatt cctttataac atccgccgcc gcgccgtgca gccacgcgcc
     5101 cgcgcaggcg gcttcaaaaa ccggcacgcc ctgtgcgagc agactgccga tgatgccgcc
     5161 caatacgtcg ccactgcccg ccgttgccaa tcccgcattg ccgctttcgt tgacatagat
     5221 ttccgtatcg gacgcggcaa ccaatgtttt gtgccccttt aaaaccacgg ttgcaccgag
     5281 aattgcccct atcttcctta ctgccgccgt ccgatccgcc tgaacctgtg caactgtcgt
     5341 tccaagcagg cgcgcggctt cggcggggtg cggcgttaaa atcaggtttt tacacccgcg
     5401 cgccagattt cgggtttcgg catcggttga taatatgttc agcgcgtccg catccaaaac
     5461 gacggcttcg tctcgattcc gcgccaacac tgccgccacc gtttccactg ccgccgcatc
     5521 tgtacccaag ccgcaacccg ccgtccaagc gtttatgcct tgacgcttgg tcaaaccgtc
     5581 tgccgtgtcc aacataatct cgggaaaacc ggcaatgacg gcaaaaggca gcgtatcctg
     5641 attgaaacct gcccgcacct taccgcagcc gagatacatt gccgccgatg ccgccaatac
     5701 gggcgcgccg ctcatccctg ccgatccgcc gactacggcg agcgtgccga aagtcccttt
     5761 gtgcgaatct tccgcacggg ctttgaaaac atggggaaaa cgcaatgccg tctgaagcat
     5821 ccggcggcgg ctttcgcctg aaagatggaa aacgggaaac atttcccctc caatcggtta
     5881 aaattaaggc tcgaaaacaa accgattcta ccgccggaaa ccgattatga aaaacaaaac
     5941 caaagtctgg gacttcccca cccgcctttt ccactggctg cttgccgcat ccctgccctt
     6001 tatgtggtat agcgcaaaag ccggcggcga tatgctgcaa tggcacacgc gcgtcgggct
     6061 gctcgtcctt ttcctgctcg tattccgcct ctgctggggc atttggggca gcgataccgc
     6121 ccgtttctcc cgtttcgtcc gaggttgggc aggtatacgc ggctatctga aaaacggcat
     6181 tcccgaacat atccagcccg gacacaaccc cttgggcgca ctgatggtcg ttgcgctttt
     6241 ggccgccgtc tcatttcaag tcggcacggg gctttttgcc gccgatgaaa acaccttcag
     6301 caccaacggc tacctcaacc atttggtttc cgaacatacg ggcagcctta tacggaaaat
     6361 ccacctcaac tttttcaagc tgctcgccgt tttttccgca gtccacatcg ccgccgtcgc
     6421 cgcataccgc atattcaaaa agaaaaacct cgtccgcccg atgataaccg gcttcaaata
     6481 catcgaaggc aaaacctcaa tccgctttgc cggcaaagcc gcgcttgccg ccgcattatc
     6541 ggttgccgcg cttgccgcag ccgccatcct gctcctgtcc tgaaacccga catcatgccg
     6601 tctgaagccc ttttctgcct ttctgacggc attttcccca ccgcacaaac ggtctgaatg
     6661 cccgaaacgg cttcaggccg tttttccgtc caaaaccgtt atatagcgga ttaacaaaaa
     6721 tcaggacaag gcggcgggcc gcaggcagta cgaatggtac ggaaccggtt cgcccggtgt
     6781 ttgggcgcct tagggaaccg ttccctttga gccggggcgg ggcaacgccg taccggtttt
     6841 tgttaatccg ctataaaacg gaaaaccgcc gcatccgcag ccgcccccca tatagctttc
     6901 agcttctaac aaatgccttt ttattattta aaatgacccg cattccgaca aaaatgccgc
     6961 gacgcaccgt atcggaaaca aggtgcgggc aaacagcaaa ggagtcagcc agtgaattat
     7021 tttcccattt tcgccaacct ctccggccgc cccgtattgg tcgtaggcgg cggcgcggtc
     7081 gccgcacgca aaatcagcct gctgccgaag gcgggcgcag aggtcggggt tgccgcaaaa
     7141 cacctgaatg ccgaactctc cgcgttggcg gcggaaaaca aaatcctgtg gcttgccaaa
     7201 gaatttcgtg ccgaacacat ccgcacggtt ttcctcatca ttgcggcaag cagcgaccaa
     7261 gccctcaacc ggcgcgtttt ccatcttgcc gaaagctgcc aaaagccggt caacgtggtg
     7321 ggcgaccgtg accattgcag cttcatcttc ccgtcggtta tcgaccgcga ccctgttcag
     7381 attgccgttt ccagctccgg cagcgcgccc gtcctcgccc gcctgctgcg tgaaaggctg
     7441 gaagccttgc tgccgccgtc tttgggcgat atggcggaaa tttcaggaag gtggcgcgat
     7501 gccggttttt atttccccgt ccaactggtc gtccgccaag atgccgacaa ggccgacggt
     7561 ttccgaggct atcaggggcg catcgaacgc ggttcggtca ccgtcgggca aaccgtccgc
     7621 atcgaaccga acgggctgac cgccgaagtg tccgaaatca tcgcgccgaa aggggaagtg
     7681 gcgcaggctt ttgccggcga agcggcaacc atccggctgg accgcgacat cgatgtttcg
     7741 cgcggcgatc tttttgtcga taaaaatttc ccgctcgccc cgcaaaaaca tccggaagcc
     7801 acgctttgct ggtttgacga acgcccgctc aacaccgcgc gcaaatacct gctcaagcac
     7861 ggcacgcaaa ccgtgtcggc aaaagtcggg gaaattgaaa gcgttttgga tgtccgcacg
     7921 ctggaacaag agaccggcgc ggaatcctta aagatgaacg acatcgccaa agtccgcatc
     7981 aaccttcaaa aacccgttac ggcaacgcct tatgcggaaa acaccgccgc cggttcgttt
     8041 atcctgatag acgaagcgac atacggtact gttgcggcag gtatgatttt atgagtgaac
     8101 acgatatgca gaacacaaat ccgccattac cgcctatgcc gcccgaaatc acgcagctcc
     8161 tgtgtcgggg ctgtccggct acgcttgggc aaaagcagga aacggggcat ccgcagggct
     8221 gcccgcgctt cagacggcat tgccggcggc agaacctttt tccgtaaccg tcctttccgc
     8281 ctcgcaaacc ggcaatgcga aatccgttgc cgacaaagcg gcggacagcc tgaaagccgc
     8341 cggcatccaa gtcaggcgcg ccgaactgaa aggctacaag gcgaaaaaca tcgccggcga
     8401 acgccgcctg ctgctggtta cgtccaccca gggcgaaggc gaaccgccgg aagaagccgt
     8461 cgtgccgcac aaactgctga acggcaaaaa agccccgaaa ttggacaaac tccaatttgc
     8521 cgtactgggt ttgggcgaca gctcctatcc gaatttctgc cgggcgggca aagatttcga
     8581 caaacgtttt gaagaattgg gcgcaaaacg cctgctcgaa cgcgttgatg cggatttgga
     8641 ctttgccgcc gccgcagacg gatggacagg ccgcattgtc gcgcgcttaa aagaagaagc
     8701 cgcaaaaaac cgggcaacgc ccgcgccgca ggcaacgtcc ccccgccggc cttcagacgg
     8761 catcggaggg caggtactgc aaggcagacc cctttcccgc cgccctgctg gccaatcaga
     8821 aaatcaccgc ccgccaatcc gataaagacg tgcgccacat cgaaatcgat ttgagcggtt
     8881 cggatttgca ctacctcccg ggcgacgcgc tcggcgtttg gtttgacaac gatccggcac
     8941 tggtcgggga aatcctagac ctgctcggca tcgatccggc aacggaaata caggcgggcg
     9001 gaaaaaccct gccggttgcc tccgcactgt tatcccattt cgaactcacg caaaacaccc
     9061 ccgcctttgt caaaggctat gccacgttcg ccgatgatga cgaactcgac cgtattgctg
     9121 ccgacaacgc cgttttgcaa ggctttgtgc aaagcacgcc gattgccggt gtgctgcacc
     9181 gcttcccggc aaaactgacg gcggaacaat tcgccggcct gctgcgcccg cttgcgccgc
     9241 gcctgtattc gatttcctcg tcgcaggcgg aagcggggga cgaagtgcac ctgaccgtcg
     9301 gcgcagtgcg tttcgaacac gaaaggcgcg ccaggtcggg cggcgcatcg ggtttctttg
     9361 ccgaccggct ggaagaggac ggcacggtgc gcgtgtttgc ggaacgcaac gacggcttca
     9421 ggctgcccga agacagccgc aagccgattg tgatgatcgg ctccggtacc ggcgtcgcac
     9481 cgttccgcgc cttcgtccaa caacgtgccg cagaaaatgc ggaaggcaga aactggctga
     9541 ttttcggcaa tccgcatttt gccgccgact tcctctatca gaccgaatgg cagcagtttg
     9601 ccaaagacgg cttcctgcac agatatgact tcgcctggtc gcgcgatcag gaagaaaaaa
     9661 tctatgtgca ggacaaaatc cgcgaacagg cggaaggact ttggcaatgg ctgcaggaag
     9721 gcgcgcatat ctatgtgtgc ggcgatgcgg caaaaatggc aaaagaagtg gaagccgcct
     9781 tgctggatgt gattatcggg gcagggcatt cggacgaaga cggcgcagaa ggatatttgg
     9841 atatgctgcg cgaagaaaaa cgctatcagc gtgatgttta ttgattaaat ataatcggga
     9901 ggaacacaaa atgaccgtac aggccaagac aaaaggtttg gcgtggcaag aaaagccgct
     9961 atccgacaac gaacgtctga aaaccgaaag caatttttta cgcggcacga ttttggacga
    10021 tttgaaagac ccgctcacgg gcggcttcaa aggcgacaac ttccaactca tccgcttcca
    10081 cagtatgtat gagcaggatg gccgcgacat ccgcaccgag gcaaaactcg agcccttgaa
    10141 atttatgctt ttgcgctgcc ggctgccggc gggatcatca aaccgtccca gtggatagaa
    10201 ctggacaaat tcgcccggga aaagagtcat taccgctcca tccggctaac caaccggcaa
    10261 accttccaat ttcacggcgt gccgaaaacc aagttgcaga cgataccccg cctcttgcac
    10321 agcctgggtt tggattccat cgctacggcg gcggatatga accgcaatgt gctttgcacc
    10381 tccaacccga tcgagtccga gctgcaccgg caggcttacg aatacgcgaa aaagatttcc
    10441 gaacacctgc tgccgcgcac gcgcggttat ctggatatgt gggtggacgg caaaaaagtt
    10501 caaagttccg acgacttcct tcaagaaggc gagccgattt tgggcaaaac ctatctgccg
    10561 cgaaaattca aaaccgcagt cgtcatcccg cccttgaacg atgtggactg ctacggtaac
    10621 gatttggatt tcgtcgccgt ttcagacggc aacggacagc ttgccggctt caatgttttg
    10681 gcgggcggcg ggctttcgac ggaacacggc aacggcaaca ccaaaaccta tccgaacatt
    10741 tcactggaac tgggtttcgt gcctccggaa cacgcactga aggccgccga agcggtggta
    10801 accacgcagc gcaacttcgg caaccgcagc caccgcaaaa acgaccgcac ccgctacacc
    10861 attcaaaata tgggcttgga caacttccgc acggaagtcg aacgccgtat gggtatgccg
    10921 ttcaaaccca tacggccgtt caagtttacc gggcgcggcg accgcatcgg ccgggtgaaa
    10981 ggcatagacg gcaactggca tttgaccctt ttcatcgaaa gcgggcgttt ggttgacgaa
    11041 ggcgggaaac agcttctgac cggcgtgttg gaaatcgcca aaatccacaa aggcggcttc
    11101 cgcatcaccg ccaaccaaaa cctcatcgtg gcaaatgtgc ccgaaggagg caaagcccgg
    11161 atcgaacagc tcgcccgctc atacgggctg atacgcgacg gtgtcagcaa gctgcgcgaa
    11221 aatgcgatgt cgtgcgcttc cttcccgacc tgcccgctgg caatggcgga agccgaacgc
    11281 gtgttgccgg acttcatcgg cgagctggat aagattatgg cggaacacgg cgcgtcggac
    11341 gactacatcg ttacccgcat taccggctgc ccgaacggct gcggacgggc gatgttggcg
    11401 gaaatcggac tggtcggcaa agccgtggga cgctacaacc tccacatcgg cggcgaccgc
    11461 gaaggcgtac gcatcccccg cctctacaaa gaaaacatca ccctgccgga aatcctttcc
    11521 gaattggaag acctgatcgg caaatgggcg gcgggacgcg ataccgacga aggtttcggc
    11581 gatttcgcca tacggacggg catcgtcaaa cccgtgttgg atgcgcccgt cgatttttgg
    11641 gacgcatcca aagccgtccc gattgcgcgc gcctgaaccc gtcccccgca ttcctgcggg
    11701 gatgttcttt tttgccgcac acgtttccat tccgcaaaat tccgatactt ggtataaaat
    11761 cccccctttt cccactctaa aaaaccgtac ccgatgccgt ctgaaacagc cctgcctttc
    11821 agacggtata ggcacaagga aacacactat gccagaatac cgctccaaaa cctccaccca
    11881 cggccgcaat atggcgggcg cgcgcgcatt gtggcgcgcc accggcgtga tggaaaccga
    11941 cttcggcaag cccatcatcg ccgttgccaa ctccttcacc caattcgtgc ccggccatgt
    12001 ccacctgcac aatatgggcc agctggttgc ccgcgagatt gaaaaagcgg gtgcaattgc
    12061 caaagaattc aacaccatcg ccatcgacga cggcatcgcc atggggcaca gcggtatgtt
    12121 gtactccctg cccagccgcg atttgattgc cgactccatc gaatatatgg tcaacgccca
    12181 ctgcgccgac gcgctggtgt gcatttccaa ctgcgacaaa atcaccccgg gaatgctgat
    12241 tgccgccatg cgcctgaaca tccccaccat cttcgtctcc ggcggcccga tggaagcggg
    12301 caaggtcatc ggcgtggcaa acatccaacc cgaacgccgt ttggacttga ttgacgccat
    12361 gatcgaatcg gcggacgaca atgtcagcaa ccggcaagtc gaagaagtcg aacaaaacgc
    12421 ctgcccgacc tgcggctcgt gttcgggtat gtttacggca aactcgatga actgcctgac
    12481 cgaagcgctc ggtctgtccc tgcccggcaa cggttcgtat ttggcgaccc acgcaggccg
    12541 caaagaattg ttcctcgaag cagggcgtat gattgtcgaa atcaccaaac gctattacga
    12601 gcaagacgac gaaaccgtgc tgccgcgcag cattgccacc aaaaaagcgt ttgaaaacgc
    12661 catgacgatg gatattgcga tgggaggcag caccaatacc atcctgcact tgctcgccgt
    12721 cgccaacgaa gcgggcgtgg atttcaaaat ggcagacatc gaccgcttaa gccgcgtcgt
    12781 gccctgcatc tgcaaaaccg cgcccaacaa ccacgactac tatatggaag acgtgcaccg
    12841 cgccggcggc atcttcgcca ttctgaaaga acttgacaaa gcgggcaaac tgcacaccga
    12901 cgtgtacacg atccacgcgc cgacactgaa agacgcgatt gaaaaatggg acgtcaccaa
    12961 tcccgaaaac acccgcgcca tcgaacgttt caaagcggct cccggcggcg tgcgcaccac
    13021 tcaggcgttc tcgcaaaacc gtatttggaa aaccctcgac cttgaccgcg aaaaaggctg
    13081 tatccgcgac gtggcacacg cctactcgca agacggcggt ttggcggtct tattcggcaa
    13141 catcgccgag cgcggctgcg tggtaaaaac cgcaggcgtg gacgagagca tcctcaaatt
    13201 caccggccgc gcgcgcgtgt ttgaaagcca ggaagccgcc gtcgaagata ttttgggcaa
    13261 ccaaatcgtc gccggcaaca tcgtcatcat ccgctacgaa ggcccgaaag gcggcccggg
    13321 tatgcaggaa atgctatacc cgacctccta cctgaaatcc aaaggcttgg gcaaagcctg
    13381 cgccctctta accgacggac gcttctccgg cggcacatca ggtttgtcca tcggacacgc
    13441 ctcgcccgaa gcggcggaag gcggcgcaat cggtttggta cacgaaggcg atacgattga
    13501 aatcgacatc cccaaacgca gcatccgcct tgtcatttcc gatgaagagc ttgccgcacg
    13561 ccgtgccgaa atggaagctc gcggcagcaa agcgtggaaa ccggaaaacc gcgaccgcta
    13621 cgtctccgcc gcattaaggg cttacggcgc gatggcgact tccgccgaca aaggcgcggt
    13681 gcgcgacgta tcgcaaatcg agagataagc cccaaaacgt gcggcaaatg ccgtctgaac
    13741 agtaaaggta tgattattaa caaatttatc tgggcgatgg cggtattttc cgcaattttg
    13801 gccctcgtta tcggcagcgg ctttaccgcg ctcgagtatg taaaagagcc gcctgccgcc
    13861 ccttatgctg tttccgcgcc gaaaaccgcc ggggtcaagc ctcggcgcat cctgccggaa
    13921 cggaatcccc cgtgctgccg attgacggtt caaaccccgc gcccgtttca aacaccggcg
    13981 atgcggacgg acaggatacg cctgacggaa aggcagcaga taccgtttcc atcatcgggg
    14041 tcggcggcat aaggtacgct gtttgacgaa ggcggtacgc cgaaaaaatg tgcaaaccac
    14101 caaaatatgc tatgcattcc gaacgccctc cgcatacggg caataccttg ccgacgcggg
    14161 attcgactac ctcagcctcg caaacaacca cagcaacggc ttcggcgcgc aaggcatcac
    14221 ggcaacggca ggcaaccttg acgaattgaa catcaaatat tcgggcatcg aaaacaggtt
    14281 tgaaaccgcc atcctgaaga aaaacggcgt gagatacggc ttcgtctcct tcgcccccaa
    14341 ccttgccgcc gtcaaactga acgattacgc caaattcaaa aaactgattc ggaaaaccaa
    14401 acaaaaaacc gacatcgtca ttgtgatgtt ccacggcggc gcggaaggga aacaggctga
    14461 acacctgccg ttcgataccg aaatcttcta tggggaaaac aggggcaacg tcgtcgagtt
    14521 tgcgcggctc gccgtcgatt ccggcgcgga tgtcgtattc gggcaggggg cgcacgttac
    14581 tcgcgccgtc gaactttacc acgaccgctt catctcttac agcggcggca actttgccac
    14641 ctacggcatc agcggcattg cgccgatttt caaaattatc accgacaaac aggggcggtt
    14701 cgtttccggc aacattatcc ccatcactca agtcggcgat aaaatcccca aaatcgaccc
    14761 tgaaaaaact gttattgagc ggattattta tctgaaccat agcgacttcc ccaacgggaa
    14821 cggactggat gtctcgcccg gcggcgacat cacgcgccgg taaaaagata ttggaaattc
    14881 cgtccgaaca aatgccgtct gaaagccttt tttcaggttt cagacggcat tcaagtttca
    14941 gacggcattt ttcaatcgtc aataatccgc tttaatcccg gaggcttttt ccacttcggc
    15001 gacctcgcct tccggctcaa actgcaaggg atacaggtta agcttttcca tcaggattct
    15061 atcgccgtcc tcatcaggat tgcctgtggt caacagcttg tcgccgtaaa aaatcgagtt
    15121 cgcacccgcc atgaagcaca tcgcctgcat cgcttcaggc atattgctgc gccctgccga
    15181 gaggcggaca taactttgcg gcatcgtaat ccgcgctacg gaaaccgtac ggacaaattc
    15241 ggtccaatcc aaatcttcgg catcggcaag cggcgtgcct tccactttga ccaaccggtt
    15301 aatcggcacg ctttcgggct gcgggtcgag gttggcgagg ctggcaatca gcccggcgcg
    15361 ttcggcgcgg gtttcgttca tcccgacgat gccgccgcag cagactttca aaccggcgtt
    15421 gcggactttg cccaaggtat ccatacggtc ttcgtgccgg cgtgtgtgga tgatgtcgtt
    15481 gtagcggtcg gggtcggtgt cgaggttgtg gttgtaataa tccaagcccg cctctttcaa
    15541 gtcttccgcc ataccttctt cgagcatacc gaacgtgccg caggtttcca tacctaagcc
    15601 cttgacggct ttgatgattg cggaaaccgt ctccacgtct ttgggtttgg ggccgcgcca
    15661 tgccgcgccc atacaaaacc ggcttgcgcc gcgcgatttg gcgattttgg ctttttcgac
    15721 gatttcatcc acatccatca tctgctcttt gcccaaattg gtgttgtggt gcgctgattg
    15781 cggacagtag gcgcaatctt cgggacaacc gccggttttg atggacaaca gcgtggaaag
    15841 ctggatttcg cgcgggttga aattttggcg gtggacttct gccgcctgat agacaagttc
    15901 taaaaacggc agtccgaaaa gtgcctcgac atcgcatttt ttccaatagc gcgcggtggg
    15961 atgaggcttg cactcggtct tacggcgcaa ggcgacgggg gatacggtca taatgtgttc
    16021 tttcgtattt acagcggcgc aatgacgctg acaggcaaac cgccggcgca atgacggcgg
    16081 ttgcgggtaa tcaaatggct gaaaacagcg gcggcaagtg taacgcgttt gaaaatacgg
    16141 ggcaaacggt ttttccgcct ttgtgccgcg cagccgggca atgccgtcta aagggctttc
    16201 agacggcatt gcccgggcta tctcaaagcg tccagcagtt tggtgtgtat tccgccgaaa
    16261 ccgccgttgc tcatcaccaa aatatggtcg ccggttcggg cgtttttcac aatttcggca
    16321 acgaaggtat cgaaatcttt accgacgcgc agcctgcagc ccaaaggcgc gagggcttcg
    16381 gcaacgtccc agtccgcgcc gccggcgtag cagaacactt ggtcggcttc tttgaggctt
    16441 gcgggcaggg cggacttcat cgtgccgagt ttcatggtgt tggaacgcgg ctcgaggacg
    16501 gcgaggatgc gcgcgccgcc gacacgttgg cgcaaacctt gaatcgtggt ttcgatggcg
    16561 gtcgggtggt gggcgaaatc gtcgtaaacg gtgatgccgt ttgccgtgcc tttgatttcc
    16621 atgcggcgtt tgacgttttt aaacgcaccc aaggcttcgc aggccgtctg aacatcgact
    16681 ccggcatggc gtgcggcagc gatgacggcg agcgcgttca tgcggttgtg tccgcccatc
    16741 aaatcccatg cgacgtgtcc ggcttttttg ccgtcaagca atacgtcgaa cgagccgtcg
    16801 gcattgactt caccaatctg ccagccgtgt ccggtgccga atttttccac cggcgtccag
    16861 cagcctttgt ccaaagtatc ttgcaggctt tgctgctgtc cgttgcagac gatgaggcct
    16921 tcggatggta cggtgcgcac gaggtggtgg aactgggtct gtatcgcgcc caagtcggcg
    16981 aagatgtcgg cgtggtcgaa ttccagattg ttcaacacgg cggtacgcgg gcgataatgc
    17041 acgaatttgg agcgtttgtc gaaaaaggcg gtgtcgtatt cgtcggcttc gatgacgaaa
    17101 aacggcgatt tgctgttcgg gtcttgacgc ggcgtttgcg gtaggcgggc ggaaacgccg
    17161 aaattttccg gtacaccgcc gatgaggaag cccggcgcga gtccggcata ttccaagacc
    17221 caggcgagca tggacgcggt ggtcgttttg ccgtgcgtcc ctgccacgcc gagtacccaa
    17281 tgatggtgca gcacgttttc agccagccat tgcgggccgg aaatataagg cagcccacgg
    17341 ttcaaaatcg cctcgaccac atccatcccg cgcctggcga cattgccgat gacgtaaata
    17401 tcggcttgaa attcctccaa ctgcgcggca tcgaagccct cgtgtacgcc tatgcccaag
    17461 gcttcgagct gggtgctcat cggcggatac atcttcgcgt cgcaaccgct gactttgaac
    17521 ccggcttctt tggcaatggc ggcaatcccg cccataaacg tgccgccgat accgataatg
    17581 tggatgtgtt tcatcagagt tccctaatcg ttcaaattct attgttcaac accgtaatgg
    17641 ttgattcggt ttcaatcaat gcggtctgat gccggcatcc gaaccgtctt tctgtgcatt
    17701 ccctttcgga ttttggacgg cttcgtccct caccgtgtcc gaataatcgc caactcctgc
    17761 gtcttccgcc ctgtctttca cggcaggcct gaccgaactg tacacccgat cccgccccaa
    17821 acccgcgttg gtacaatgtt ttcccggagt ttggctgtaa ctggttttac cattatggcg
    17881 gcactcgtaa acatttgccg aaaatacggt ctgaaccgct gcaacaagca atatacttcc
    17941 aatcatttta gatacgcgca tattaaactc ctttattgtt tctcttcaaa aaaaggaatg
    18001 tatttcaata gaacttcatc taattctttc attccttgct cataattgaa cgggtattcc
    18061 caaccatccc ctactttgat ttcagaaatg tctttaaact tccgcttaag ggcggacact
    18121 ttgtccatat cgtcatcaat ttcctgtttg ccgaacggta ttttccgctt ttccaaaacc
    18181 tcgtcataat aacaaggtaa attcagcaat agcccccaag cgctatttgc gattacgact
    18241 aatctatatt tttccctagt taagtaatct gcaccgctat catagattgc ccttgtcgga
    18301 tcataaaaaa attcgcaatt tttcatagcg tccccacact ttaaccgatt cgcaatttta
    18361 tatccgcgat tttgcaagct gcccataaaa accgattgcc tgccaagccg acactacttg
    18421 ttaaagatgc ccgataatgc cgcaactctt ccgtatttac cgacaaacgc cctatttgct
    18481 tagagctgtt ttgttttcca caaaacttta atccgttgat ttaacaaatt ttttaccaaa
    18541 ttctttattt gttcggattc cattagcacg attttgagca atatctgtca aaaccatttc
    18601 caccatatcc aaaacaaaac cgccgccgcc acggcattgg acgataagat gagtttccac
    18661 tgcccgtcct tccgcaatgc cgccttcttg ggcttcaaag cacccgcata aaaaaacggt
    18721 gacagcagca tagaagcgat acataagaaa atcaatacgc cgtaatagat tttttcagac
    18781 ggcatttctc ttcctcaata cgccgcccgt tggcgcatct gttcaaacag gcagattgtc
    18841 gccgccatcg cgacatttaa agactcggtt gcatcgtgca tcggtatcct gacacacttg
    18901 tccgccctgt ctaaaactgc tttaccgaca cccgcacctt cgttgccgaa cacccaagct
    18961 gtcggttcgc acaaatcttc gccgtacaaa accgcctgct tttcctcgcg caacgcggtg
    19021 gcaaacacac ggtcttcata gtgcgccaac catatttcca aatccgcctg cgaataaatg
    19081 tccaacaaga aatgcgcgcc catacccgcg cgtaatacct tgggcgacca cgcgtccgca
    19141 caacccctgc ccaaaacgac cgtacctacc cctgccgccg ccgcactccg taagacggtg
    19201 ccgacattgc ccggatcttg cacgccgtcc aaaaccgcac agtcgccttt gtccggcaag
    19261 gttccgccca atgggatatc aatcagtgta agtatatcgt ctgcacaact caagctgctg
    19321 atttttttca atatgccgtc tgaaacggaa aaaatcccgt cttccggcaa aaccgctgtc
    19381 aatttaagga cttcttcaga cggcatttta gcttcgggaa tatatacccc gaccggcttt
    19441 ctgccggatt gcaggaaaac ctgaagcagg tgcacgccct ccaaaacggt ttgggcatac
    19501 tgccgtctga actttccttg cgacaacagg cggtgcaggt gtctgatatg ttcattattg
    19561 gtcgaactga tgtgtttcac ttcacaagcc ttttagaaat atgcaaccgg acaatacccg
    19621 actgaacgcc gccgaaatct gtttgcgata ttatcaaaat aaaatgggaa agtcaggatg
    19681 tacgggcaaa atgccgtctg aaaccgagat ttgaaaatcc gcacaacagt ttgccaacgc
    19741 caatggacgg tttccgctgt cggcaacgag cggccaaatt gggcgcagga aaggcagaac
    19801 ccgtttcccc tgaaggattt ttttcacatt tttatggatg ccgccgacag ccaacgtatc
    19861 cgatgccgct accgtcctca aaatcccgtc ctgttccaac atggcttcag gaagtccgaa
    19921 cggatgccgc ttcaacacaa atctatgttc tgtcagtatt tttttgagat ttccgcttat
    19981 cgtcccatct ttcagaaaag ccggttttgc caactgttcc gacgcaaaca caaacagcct
    20041 gcctgcataa tgatgcaatt caaagccttg caagttccaa cgtccggttt ttgcctccgt
    20101 caaaacgcgg gcaatgtcgg caagtgcgtt ctgattcggc acggggatgc cgttttcctt
    20161 tagaaaattc cgcaaaatat gggttttccg gcgcggggaa aacgtcagcc accgcgccgt
    20221 atcgaaataa ccggccccgc aaacccaacg gcagtcttga acgacgacct cttccaacaa
    20281 agccaaatct tcctgcaaag cgcggacatt gttcagcaca tgccgcccga aatggggaat
    20341 ctgcgccgaa agttcgggca aaatacggtg ccggaagcgg tttcgcaaat aagccgtatc
    20401 ggtattgctt tcatcctcga tattcggcaa accgtgtttt cgggcataat cccatatgtc
    20461 ttggcgcgaa aaaggcagca aaggccgcca gataatgccg ttttccccca aagggcggac
    20521 ggcgggcata gccgccaaag cgcgcaaacc tccgccgcgc gcgactgcca gcataaaggt
    20581 ttcgatttga tcgtccctgt ggtgcgccaa cgccaaaacg tcaaagcctt tttcggcaaa
    20641 ctcggcataa cgcttttgcc ttgccgccgc ctcgatgccc aaaccgtttt tttccacgca
    20701 gaccttaacc gtttccagcc ccacccctag catatcgcaa taattccggc agaaatccgc
    20761 ccaatcgtcg gctcggggac tcaagccgtg atggatgtgc aatgcctccg gcacaaatcc
    20821 tgcccgcttc ccggcacaaa ccagcagatg cagcaaaacg acggaatcca agccgccgct
    20881 taatgccact gctgtttttt ttccattcag accttgagga aaacaatcct tcaagcattg
    20941 ctcaaacgca tctaaagtca gcacggcttc atcccgataa agaaaaagca agcagtttca
    21001 gacaaactgc ttgctgcttg ttctacccga attattgttc cgaaaatttg ccgtaagcca
    21061 taatgcggtc gaaacggcgc gaaagcaaat cggcaagcgg gatgctttgc gcttcgtgca
    21121 gttgtttttc caaaacggct tttacgtttt tcatggtttg cccgaaatcc ctatgcgcac
    21181 cacccaaagg ctctttaatg acggtatcga ccaagtccag cttttgcagg cggtcggcag
    21241 taatacccaa agcctgtgcc gcatcagccg ccttttcggc ggttttccac aaaatcgaag
    21301 cacagccttc gggggaaata acggaatagg tcgaatattg cagcatattg acgtaatcgc
    21361 ctacggcgac cgccaacgca ccgcccgaac cgccttcgcc gatgacggta cacaaaacgg
    21421 gaacgcgcag tcgggtcagt tcgtacaggt ttttgccgat ggcttccgac tgcccgcgtt
    21481 cttccgcgcc gatgccggga tacgcgcccg gcgtatcgat aaaggtcatg acgggcaagc
    21541 cgaatttttc cgccgttttc atcaggcgca gggctttgcg gtagccttca ggacggggca
    21601 taccgaagtt gcggcggatt ttttctttgg tgtcgcgccc tttctgatgc ccgacgacca
    21661 caacgctttg tccgttgaaa cgcgccaatc cgccgacaat cgcatgatcg tcggcaaagt
    21721 ggcggtcgcc gtgcagctct tcaaaatcgg taaacagcgc gtcaatgtaa tccaaagtat
    21781 agggacgctg cggatgccgg gaaacctgtg aaatttgagc gggtgtgagt ttgctgaaaa
    21841 tcgatttggt caaatcgttg ctttttttct gcaaacggtg tatttcgtcc gaaatatcga
    21901 cggcagactc gtcttggacg aaacgcagct catcgatttt gttggtcagt tcggctatgg
    21961 gttgttcaaa atccaaaaaa acaggtttca taaaatgaag ctctcatcaa cagattctgc
    22021 cgctatcata cgctatttgt gcatattccg cagttaattt attgtccaaa acggcttttc
    22081 cacaccctct ttttgtgttc ggacagcatg gaaaacagcg gattagtgca tatatactag
    22141 ttataaggcg atgtatctta tgctaaactc gaaacaaatt caattatttc aaacaaaatc
    22201 gtatgaaaga caagcacgat tcttccgcca tgcggctgga caaatggctt tgggcggcac
    22261 gttttttcaa gacccgttcc cttgcgcaaa agcatatcga actgggtagg gttcaagtaa
    22321 acggctcgaa agtcaaaaac agtaaaacca tagacatcgg cgatattatc gacctgacgc
    22381 tcaattccct tccccataaa atcaaggtta aaggtttgaa ccaccaacgc cgcccggcac
    22441 ccgaggcgcg gcttctgtat gaagaagacg cgaaaacggc agcattgagg gaagagtgca
    22501 aacagctcga ccaattcagc cgcatcactt ccgcccatcc cgacggcaga ccgaccaagc
    22561 gcgaccgccg ccaactggac aggctgaaaa aaggagactg gtaaaaccat tcccgctgca
    22621 aacagaagca cgaaccgcgt accgccgcat ttcagacggc attcctgaaa cttattccgc
    22681 ctcgtctatc catgcctgct gcacggcttc aaggatgcgt tcgccgcaac gggcgggatc
    22741 gtcgtcaaat tcgggcaatg ccataatcag gtcgcgcagt tgggtaaagc gcacggtttt
    22801 gggatcgatg gcttcgccgt gcaggtcgta gagttcttcg gcgatgcgcc gggtatcggt
    22861 ccatttcata taaatttcct tttatcgtcc ggcattgaag ccgtgtagaa tatgtttcgg
    22921 caaaactgcc gccccttgag gatggctgaa tgtttcaact cgccggacgg cacatcaagc
    22981 accaccttat gtgtcgtcct gatccgggag gagttacccc tcccaaacaa atctgattct
    23041 accgccctga agagcggggt ttcaaccgat aaggaagatt gatgaacaat atgtttgccg
    23101 caaaattgtc cgaactggtt tataccgctt ccgaccatcc cgactccctg tcggagatgg
    23161 aggagtttga ccgcctgatt ctgctgatac gcaaactgta tcaaatattg gacgggcaac
    23221 ataccctcta cagagtaacg gtttgcctta actaccggag caggcgcggt ctgattgctt
    23281 tggataaagc ggctgtcggt tgcgatgcgg caatgttgcg cgcccggcgt tggcgcatct
    23341 gcatgcagat tgcggaacgc ctttccgagt cggtcgggcg gcttcacggt tacggcagaa
    23401 agcgtgtccg ccgcctgccc cgagcttgaa ggacgctatc tcgaccttgt ccgccgcgcc
    23461 gccgtctctt tcggtttcgc gcaaggacag gcaaatccgg caggcgggca ggaagaaacg
    23521 caagccgcct cagatgccgt atcgggtcgt tccgtcaaaa aaccgataaa aatcaatata
    23581 gaatcgaaag ttccgtcttc acaaacgtct gtttttcggt aattgcgcct tatccgcgca
    23641 acaatgccgt ctgaagcgtg ccggcttcag acggcctgtt ttcagtgttc ttcgcgcgcg
    23701 tggttgatgg tgtatttagg gatttccaca atcaaatcct cgccggcaac aaccgcctga
    23761 caactcaggc gcgaatcggc ttccaaaccc caagcctgat cgagcaggtc ttcttccaat
    23821 tcggccggct cttccaaact gtcgaaacct ttgcggataa tcacgtggca ggtggtgcag
    23881 gcgcaggatt tttcgcaggc gtgatcgact tcgatgtcgt ggtcgagcag cacgtcgagg
    23941 acggttttgc cttcgggcgc gttatcgatg actgcaccct cggggcataa tgttgcgtgt
    24001 ggaagtacgg tgattttggg catttttatt gtctttctgt aaaaagtatt tgtgtccgtt
    24061 tttaggaaaa ctgtctgcct aatgtcgcgg ttcgagcagg tcggcaacat tttgccagcc
    24121 cgtcaggcgg gcaaaatcga gggcggtata gccttccgat gtctttgagg caggatttgc
    24181 gccgtattca agcatggctt gaaccataag tgcatttcct gccatggctt catgatgaag
    24241 cggcgtatag ccgtcttcgt cctgctcatg cagaaaatcc ggattttggg acaactgttg
    24301 ccgaaaatcg tcaatacagt tccggcacat cggatgctct tccaaatttt cgggatgttc
    24361 tttttcttca tacaccggca atacctgccc gggatcgccg aaatcgattc cccaggcggt
    24421 atcgtgttcg gtacgctcct cttccgtcat ctgtccgcgc attgcctgta cggtaaagcc
    24481 gccgtagggg atgccgttgc acacgaacat ccagtcgctg atgtcgccaa ccggaacgca
    24541 aacgctttcg ccttgttcga cattggtcag tccgccgggt tcattgttca gcacaccgta
    24601 aatataaaga ccgtcaaaat aaatatcgtc gatccacata tgttcgcaaa tttcgccgtc
    24661 ttcgccgtct tggaaaaaag ggactttgac catggcaaaa tccaaggcgg aaataatgcg
    24721 gcggcgttcc caaaaaagct cgcgccaaaa gtatttgaat gttttacggg cgcgttcgcc
    24781 ggcacggttt accggttcgt ctgcctgttc tacataataa atgacggaat cgcccatcat
    24841 actgctcctc aacgtgtacg gtatctgttt gcaccttacc gcggttttct acctccggca
    24901 tccgattcgg atttgaaaag ttccaaatat tcggaatagg ctttttccaa atccgcccgc
    24961 gaaaaacagg tgtaaatatg gatgccgtct gcaatttcgt cggtcagcct cgtgccgctt
    25021 ttgccgaaat atgcttcttt aaaaaggcgg attttcgatt ttttcggctt gtaggatgcc
    25081 gcgtccgcca aaagccgggg cagcgcgtaa gggtattccc ggtgtccgcg ccgtaccaaa
    25141 tcaagcagtt gtttgcggcc ggacccggca ttcccttcct gccggcgttc gtggacgctt
    25201 ttttcaaatt ccgattccgc cgtttcgttt ttcgggaaca cgatttcgcc gcgaagcgat
    25261 aaaaacaaac gtttcacaag gttttcgtcc acggcgaaca attcggtatc gcctatcctg
    25321 cttttgctga aaatttcatg gagcaggttt tttttctttg taattgtcgg ttttgacggc
    25381 gaggaggcgt tccaacccgg caacgttcgc atagccgttg ttttccaaat ggcgcattct
    25441 gccttcaaaa ttgcttacgt ccgaaatgcc gattttatat agtgaattaa caaaaatcag
    25501 gacaaggcgg cgagccgcag acagtacaaa tagtacggca aggcgagcca acgccgtacc
    25561 ggtttaaatt taattcacta tacacgccct tgatgacggt tttcatcgaa tagacaatgc
    25621 ctgacttttc catatcgatg tttttcaagt gttttcgatc cttcagacgg catcaggtta
    25681 tatctatgcc gtctgaaacc gtttaagtat caaatattat cgacactctg acctgtcagc
    25741 gcacgttgga tgttgcggtt catgcgcttg gcggcgaaat tgtcggtgct gcggctgagt
    25801 ttggcgacag ctgcacggat gtcttcagct ttaccgtctt tcagacggcc ttgcaaatcg
    25861 gcgatgtctc gctggatttg tgcaaactct tcggcatcca gcaaatcgct gtccaactcg
    25921 agggcggcgt tgacggcatc ggtcaggctt tcggcttcga ccacggcttc ggcgcgggcg
    25981 cgtgccgcca tatcttcggc ggcgttgccc atgctgtctt tgagcatttg ggtgatggtg
    26041 tcatcgtcca aaccgtagga gggtttgact tcgatttgcg cctgtacgcc ggtgctttgt
    26101 tcttgggcgg aaacggacag caaaccgtcg gcatcgattt ggaaggtaac gcggatacgc
    26161 gccgcacccg ccgccatagg cggaatgccg cgcagggtaa atttggcaag gctgcggcag
    26221 tcggaaacga gctcgcgttc gccttgcacg acgtgtatcg tcatcgcggt ctgaccgtct
    26281 ttgaaggtgg taaagtcctg cgcgcgcgcg gtggggatgg tggaattgcg cgggatgatt
    26341 ttttccgcca agccgccgta ggtttccaaa ccgagcgaca agggggtaac gtccaacagc
    26401 agccattcgc cgtcggcttt gttgcctgcg aggacgtttg cctgtatggc tgcgccgagc
    26461 gcgacgactt cgtcgggatt gaggttgttc agcggggttt gtccgaaaaa ggtggcgact
    26521 gcctgttgga cgtgcggcat acgggtcgaa ccgccgacca taaccacgcc tttgacttcg
    26581 tttttaccga caccggcatc tttcaacgcc tgtttgaccg gctcgaacgt tttcatcacc
    26641 aaatgctgcg tcaggttgtg gaactcggcg cgactgatgc ttgtgtcgat tgccataccg
    26701 tctgaaaacg tcgcctgaat gcgcgcttcg gtttgcgtgg tcaattgttc tttggcggcg
    26761 cggacgagcg agagcaggag ttggctgtct tgttcgttga gttgggagag tctgttttgt
    26821 tcgagcaggt agcagaacaa acgatggtcg aagtcatcgc cgcccaacgc gctgttgccg
    26881 ccggtggctt tgacttcaaa cagtcctttg gtcagttgca acacggatac gtcgaatgtg
    26941 ccgccgccca aatcgtacac gacaaacgtg ccttccgagg cgttgtccag cccgtaggcg
    27001 attgcggcgg cggtaggttc gttgagcagg cgcaatacgt tcaaaccggc cagacgcgcg
    27061 gcatctttgg tggcctggcg ttgcgcgtcg tcgaaatagg cggggacggt aatcaccacg
    27121 ccgaccaaat cgccgcccaa ggtttcttcg gcacgcaatt tgagggtttt gaggatttcc
    27181 gccgacactt cgacaggcgt tttcaccccc tgccgcgtat gcagttcgat aaagcgttga
    27241 ttgtcgccga aacggtaagg caggtaatgc gtattttgat gcagatcggc aagagtccgc
    27301 ccgataaggc gtttggcgga gctgacggtg ttcagcgggt cggttttttg ggcggacagg
    27361 gcggttttgc cgacttcaat gccgccgttt tccagatagc ggacgacgga aggcagggta
    27421 acgcgcccgt cggcatcggg caggcaggcg gcactgccgc tgcggacggt ggcgaccaag
    27481 ctgttggtcg tacccaaatc gatgcctgcc gccaaacggt gccggtgcgg ggcggcggac
    27541 atacccggtt ctgaaatctg caaaagagcc ataaattttg cctgtcggtg cggtgtgtaa
    27601 aggaaggaag gcggcatttt aacagaattt gcgccgatag ttgagcaaac cgctaaggat
    27661 tgggccgtgc cgccgaaatt gccctctacc gccaaatacc gataaaatcc gcctttcccg
    27721 cccatcatta ggaatataaa tcgtgaaagg actcgactat tgccgccaaa aagcagagga
    27781 aagccgctcc agttttttgt cgggcttccg tttcctgact caggaaaaac aggatgcggt
    27841 aacggttctg tatgtctttt gccgcgaatt ggacgatgtg gttgacgaat gttccaaccc
    27901 cgatgttgca caggcaacat tgaactggtg gcgcggcgat ttggacaatg cgttcggcgg
    27961 cgcgatgccg gaacaccccg tcaatcaggc cttgcggcag gttaaggaaa ccttcaagct
    28021 gccgaaatat gaactggaag ccttaatcga cgggatgcag atggatttgg ttcaggcgcg
    28081 ttacggcagt tttgaggaat tgaaactgta ttgccgccgc gtcgcgggcg tggtcggctg
    28141 cctgattgcg cggattttgg ggttttcaga cggcaaaacg ctggaatacg cggataaaat
    28201 gggtcttgcg ctgcaactga ccaacatcat ccgcgatgtc ggcgaagatg cgcgcagggg
    28261 acggatttac ctgccgatgg aggaaatgca gcggtttgac gtacccgcaa gcgtgatttt
    28321 gcaatgcagc ccgacgggca attttgccga attgatggcg ttccaaatca aacgcgcccg
    28381 tgaaacctac cgcgaagccg tatcgctgct gcctgatgcc gataaaaaag cccaaaaagt
    28441 cggactggtt atggcggcgg tttattacgc gctgttgaac gaaatcgacc gagacggcgc
    28501 gcaaaacgtc ctcaaataca aaatcgccct cccttcgccg cgcaaaaaac gcattgccct
    28561 gaaaacctgg ttattcggat tcaaaccgcg ccccggcacg ccggaacggg cataaggcgc
    28621 atactgccct tcaggaaaac ccaacccccg ccgccccgaa aaacggggtt tcaaccattg
    28681 gaggaataaa tgatgaacac gccgcatccg cgcccgaaaa tcgccgtcat cggcgcaggc
    28741 tgggccggct tgtccgccgc cgtcaccttg gcgcggcacg ccgacgttac cctgtttgaa
    28801 gccggccggc aggcgggcgg aagggcgcgc acactggccg gaaataccga cggtttcggt
    28861 tttttggaca acgggcagca cattttgctc ggcgcatacc ggggcgtgtt gcgcctgatg
    28921 aaaaccatcg gttcagaccc ccgtgccgcc tttttgcgcg taccgctgca ctggcatatg
    28981 cacggcggtt tgcagttccg cgccctcccc ctgcccgcgc cgctgcatat tttgggcggc
    29041 gtgctgcttg cccggcgcgt accgtccgca ttcaaagcca aactgcttgc cgatatgtcc
    29101 gatttgcaga agtccgcacg cctcggacag cccgacacga cagttgcaca atggctgaaa
    29161 cagcggaacg tgccgcgtgc cgccgtaatg cagttttggc agcccttggt ctggggcgcg
    29221 ctcaacacgc ctttggaaac cgcaagcctg cgcgtgttgt gcaacgtttt gtccgacggc
    29281 gtgctgacga aaaaatccgg cagcgactat ctcctaccca aacaggattt gggcgcaatc
    29341 gtcgccgaac ccgccttggc ggagcttcaa cggctcggcg cggacatccg cctcgaaacg
    29401 cgcgtatgcc gtctgaacac cctcccggac ggaaaagtcc tcgtcaacgg cgaagccttc
    29461 gatgccgcca tacttgccac cgcgccctac cacgccgccg cgctcctgcc cgaaggcacg
    29521 cccgaacacg ttcagacggc atatcaaaac cttcgctacc acgccattac caccgtctat
    29581 ctgcgctacg ccgaacccgt ccgcctgccc gccccgctga ccggcattgc cgacggcacg
    29641 gcacaatggc tgctttgccg gggcaggctc ggactgcccg aaaacgaagt ctccgccgtc
    29701 attagcgttt ccgaccgcgt cggcgcgttt gcaaaccggg cgtgggcgga caaagcccac
    29761 gccgacctca gacgcatcct tccgcatttg ggcgaacccg aagccgtgcg cgtcatcacc
    29821 gaaaaacgcg ccacaaccgc aaccgatgcc ccgccgccgg atttgtcgtg gctgcaccgg
    29881 caccgtatct tccccgccgg cgactacctc cacccgcact accccgccac gctcgaagcc
    29941 gccgtacaat caggtttcgc gtcggcggaa gcctgcctgc aaagcctgaa cgatgccgtc
    30001 tgaaaacgcc ggccggcatc gggacgcttt acaaggtgcg gcaaaacgct aaaatacatt
    30061 aaccgtcaaa cccaaaggac aaccatgccg acactgaccg acaaaaccat cttagttacc
    30121 ggcgcatcgc aaggcttggg cgaacaggtc gccaaagcct acgccgccga aggggcgacc
    30181 gtaattctgg tggcacgcca tcagaaaaaa ctggaaaaag cctatgacgc gattgtcgaa
    30241 gccggacacc ccgaaccctt cgccatccgt ttcgacctga tgagtgcgga agaaaaagaa
    30301 ttcgaacggt ttgccgccac cattgccgaa gccacgcaag gcaaactgga cggcatcgtc
    30361 cactgcgccg gctattttta cgccctctcg ccgctggatt tccaaaccgt cgccgaatgg
    30421 gtcaaccaat accgcatcaa caccgtcgcc ccgatggggc tgacccgcgc cctgttcccg
    30481 ctgctgaagc agtcgcccga cgcgtccgtc atcttcgtcg gcgaaagcca cggcgaaaca
    30541 cccaaagcct actggggcgg cttcggcgcg tccaaagccg cgttgaacta cctgtgcaaa
    30601 gtcgccgccg acgaatggga acgcttcggc aacctgcgcg ccaacgtcct cgtccccggc
    30661 cccatcaatt ccccgcaacg catcaaatcc catccgggcg aagccagaag cgaacgcaaa
    30721 agctacgggg acgtactgcc cgcatttgtc tggtgggcaa gtgccgaaag caaagggcgg
    30781 agcggcgaaa tcgtttacct ctaaatccgg aaaaggcagg catcccgccc gcgccgcgct
    30841 tccgttttga aaaatatccg taccttccgc gcgcatccgt cccgcacaga caaacacctt
    30901 gacttaaatt tacataagga caataatgat aacccgttat ttccctaact ggaccttagg
    30961 gtaaatttta tgaaaaccgt ttccgccgcc atcgcttttg ccgccgctgc cgtttcactg
    31021 accggctgtg cgaccgagtc ctcacgcagc ctcgaggttg caaaagtcgc ctcctgcaat
    31081 acgcaatatc acggtgttcg caccccgatt tccgtcggaa cattcgacaa ccgctccagc
    31141 ttccaaaaag gcattttctc cgacagtgaa gaccgtctgg gcagccaggc aaaaaccatc
    31201 ctggtaacac acctgcaaca aaccaaccgc ttcaacgtac tgaaccgcac caaccttagc
    31261 gcattgaaac aggaatccgg catttccggc aaagcgcaga acctgaaagg cgcagattat
    31321 atcgttaccg gcgatgtaac cgaattcgga cgcagagatg tcggcgatca tcagctcttc
    31381 ggcattttgg gtcgcggcaa atcgcaaatc gcctatgcaa aagtggctct gaatatcgtc
    31441 aacgtcaata cttccgaaat cgtctattcc acacagggcg cgggcgaata cgcactttcc
    31501 aaccgcgaaa tcatcggttt cggcggcact tccggctacg atgcgacttt gaacggcaaa
    31561 gttttagact tggcaatccg cgaagccgtc gacaacttgg ttcaggctgt cgacaacggc
    31621 gcatggcaat ccaaccgtta aggagaaatg atgaatccga aaaccttggg ccgtttgtcg
    31681 ctgtgtgcgg cggtcttggc tctgaccgcc tgcggcggcg ggcataaaaa cctgtattat
    31741 tacggcggtt atcccgatac cgtctatgaa ggtttgaaaa acgacgacac ttcgttgggc
    31801 aagcagaccg aaaagatgga aaaatacttt gcggaagccg ccaacaaaaa aaatgaatgc
    31861 cgccccgggt gcgcacgccc atttgggact gctgctttcc cgttcgggag acaaagaggg
    31921 cgcgttccgc caatttgaag aagagaaaag gctgtttccc gaatcgggcg tatttatgga
    31981 cttcctgatg aaaaccggta aaggaggcaa gcgatgaaac ctttgatttt agggcttgcc
    32041 gccgtgttgg ctctgtctgc ctgccaagtt cgaaaagctc ccgacctcga ctacacgtca
    32101 ttcaaagaaa gcaaaccggc ttcaattttg gtggttccgc cgctgaacga gtcgcctgat
    32161 gtcaacggca cttgggggat gctggcttcg accgccgcgc cgatttccga agccggctat
    32221 tacgtcttcc ccgccgcagt cgtggaggaa accttcaaag aaaacggctt gaccaatgcc
    32281 gccgatattc acgccgtccg gccggaaaaa ctgcatcaaa ttttcggcaa tgatgcggtt
    32341 ttgtacatta cggttaccga atacggcact tcatatcaaa ttttagacag cgtgacgacc
    32401 gtatccgcca aagcacggct ggtcgattcc cgcaacggga aagagttgtg gtcgggttcg
    32461 gccagcatcc gcgaaggcag caacaacagc aacagcggcc tgttgggggc tttggtcggc
    32521 gcagtggtca atcagattgc caacagcctg accgaccgcg gttatcaggt ttccaaaacc
    32581 gccgcataca acctactgtc gccctattcc cgcaacggta tcttgaaagg tccgagattc
    32641 gtcgaagagc agcccaaata agcatatgcc gtctgaacct ctccgagctt cagacggcat
    32701 atgcgaatct tgatacttgc aagccgcacc gcccaaaatc aggcgaccgg tttcaatatc
    32761 cgaacaaggt tcgggttctg tattttgcag aacccgaacc ttttattcaa atttaaactt
    32821 aattccggcg tggttgtatg gtaaattata gtggattaaa tttaaaccag tacggcgttg
    32881 cctcgccttg ccgtactatc tgtactgtct gcggcttcgt cgccttgtcc tgatttttgt
    32941 taatccacta taacaagcaa tatgccctga ccggcaaaac ttccccaaac ttcatcaaaa
    33001 aatcaactgt acccgtcttt ttcagacggc attgataccg taagagattt cccatgcaaa
    33061 accggcaaac cgagctttat tccacgccgt cttggctgtt gcagacctta ttgatgatta
    33121 ccgccgcctc ggcggtgatt ctgtttttcg cgcgcaacac gcgcttgggg cgggagtttg
    33181 cctatatcct gcgcctttgc ctgacaccga aaagcacggt caaagtattg ctgctgatta
    33241 cggcaatggt tacgctgctg ttaaccgaag tgcggttgaa tgtgttgagt accttcatgt
    33301 ccaaaggact ttacgattcg atgcaggatt tgaatgcttc ggcattttgg atgtttgcag
    33361 cgatgaacgc cggcgtggta ctgatacggg cgtttaacaa cgtcgttaac gactttctcg
    33421 attaaggctt ggcgattaaa tggtcggaac ggctcaatga agttttgact tcgcgctggc
    33481 ttgccgacaa aaactactac cgcctgcaaa tgcgccgcca cgcgccggac aacatcgacc
    33541 agcgtatcca acaggatgcg caggaattca tcgcttcgac catcgaattt gtgcgcggca
    33601 tggtcaattc ggtcgttact tctctggaat ttgccgttgt tttgtggggc ttggcaggca
    33661 tcctgaccgt gttcggcttc gacattccgc acggcattgt ttggtttgtc tttattttca
    33721 tgattttggc gatctttatc gccatgtgga taggcaaccc tttgattcgt tacaattatg
    33781 aaaacaaaaa actcaacggc gactaccgtt attccctcat cctcgtacgc gaccacgccg
    33841 aaagcgtggc gttttacagc ggcgaacaac acgaacacgg ccggctttcc gaccgcttta
    33901 aagccattat ccgcaaccgt tggcgcatcg cgcggcaaag cgtctgctta agcggcttta
    33961 acgatatgtt caccaacggc atcaaactct tcctgattat tttgcaagct ccgcgcctgt
    34021 ttgccggaca aatcaaaatc ggcgacatcc agcagaccgt ccaagccttc gcgcgactgc
    34081 aaaacgcgct gtctttcttc cgaatgttct acaacaaatt caccgcctgc cacgcccgac
    34141 tggagcgtct gtacgtcttt ttgctgagta ccgaagaaca acacggcgcg cagcaacccg
    34201 agattagcga agtttcagac ggcattgcac tggaaaacgt cgccctgttc cgccacaacg
    34261 gcgaagtttt attggacggc atcaacatca aactcaaaag cggcgattcc ctgctgatac
    34321 gcggcccgag cggtttaggc aaaacctcgc tgctgcgcgc gctggcgggg ctttggccgt
    34381 tcggcagcag cggcaaagtc agccgtccgc cgcatcaagg catcctcttc ctgccgcaac
    34441 gcccgtacac ggcacaaggc agcctgcgcg acgcggtttg ttaccccgat atcgacaaac
    34501 agcatcccga gttagccgaa gccatgaaca cctgccgctt gggttatctg attgacaaat
    34561 tggataaaac agacggctgg cagcacaaac tctcccccgg cgaactgcaa cgcgtcgcct
    34621 tcgtccgcgc cctgctctcc aagcccaaaa tcgtcctgct cgacgaagcc gccgccgcct
    34681 tggacgaacc ggccgaagcc tagctctacc gcgccttaaa gcaaaaactg cccgacagca
    34741 tcatcagtat cggacaccga agtacgctca acgcgttccg caatatgcac ctcgatgtcg
    34801 gcaacgctgc ctgcggctga acgatgccgt ctgaagccgt tttcagacgg catcgttcaa
    34861 attggaaaga cacgcgaaaa caacgtacaa taaacccgat tccaacccat ccggtatgcc
    34921 tttgtgaacc agcttatttt cgactttgcc gcacacgact atccgagttt cgacaaattc
    34981 ctcggcacgg aaaatgccga actggtctat gtgttacagc ataaacacga cccgtttatc
    35041 tacgtctggg gcgaagaagg cgcgggcaaa agccatctgt tgcaggcgtg ggtcgcacag
    35101 gcgctcgaag ccggcaaaaa cgccgcctat atcgatgccg cctccatgcc gctgaccgat
    35161 gccgcgtttg aggcagaata cctcgccgtc gatcaagtcg aaaaactggg caacgaagaa
    35221 caagccctgc ttttttccat cttcaaccgc ttccgcaaca gcggcaaagg ctttttgctg
    35281 ctcggttcgg aatacacgcc ccagcagctc gtcatccgcg aagatttgcg gacgcgtatg
    35341 gcatactgcc tcgtttacga agtcaagcca ctaaccgacc aagaaaaaat cgacgcgctc
    35401 gccaatatgg cggcggcacg ccaagtaacc gtcgattccg aaattttcga atacctgctc
    35461 aaacactggc ggcgcgatat ggacagcctg atgatgatgc tcgacacgct ggacaactac
    35521 gccgtaacca tgggcaaacg catcaccctg ccgctgcttc gccagctttt gaaacaacag
    35581 gaaacccaat gaaaaacctc gccatcttcg acctcgacaa caccctcatc aacaccgatt
    35641 ccgaccacgc ctggccgcaa tacctcatca aaaaagggtt ggttgacgct gccgaaaccg
    35701 aagcgcaaaa cgaaaaattc taccgcgact accaaaacgg ctgcctcgac atcgacgcat
    35761 tcctcaaact ccacctcgcc ccgctcgccc gttacagcaa agaagagctg gcggagtttc
    35821 accgcgaatt tatggcggaa tacatcatcc cccacatctc gcctatgcag cgtatgctgg
    35881 tgcagagcca ccaaatggca ggcgacgaaa ccctcgtgat ttcctcaacc aacgagttca
    35941 tcatcacccc cgtctgccgc cttttcggca tcgccaacat gatcggcaca caactcgaaa
    36001 ccggtcccga cggccgctac accggcaatt acatcggcac gcccagcctc aaagaaggca
    36061 aaatcacccg tctgaaccaa tggctcgccg aacgcggcga aacgctcgaa agctacggca
    36121 aaacctattt ttacagcgac tccaaaaacg acctgccgct gctgcgcctc gtcagcgaac
    36181 ccgtcgccgt caaccccgat gccgaactgg aaaaagaagc caaagaaaaa ggctggccgg
    36241 ttttgaattt caaatgacgc aatgccgtct gaagcccgat tcggcgttca gacggcattg
    36301 aggctgaaat cccaaaaacc caaagccaca ggaattcatc cggaaaaaca aaaaacacag
    36361 gaattcatcg ggaaaaacaa caatctttcc accgtcattc ccacgaaagt gggaatcttg
    36421 atccgtccgc acggaaactt atatttcgtc attccaccaa tcccacacca cctttaccgg
    36481 ttcaaatccg cacaaaaatc cacatcccgt cattcccacg aaagtgggaa tctaggacgt
    36541 aaaatctaaa gaaacctttt tttcccgata agtttccgtg ccgacaggtc tggattcccg
    36601 cctgcgcggg aatgacgaaa tttcaaagtt atggcgttat cggaaaaaca gaaaatcaaa
    36661 gccggagaat ttatccaaaa caaccggatt tcaaaaaaac caaattcccg gcgggaatga
    36721 cggattttag gtttctgttg tggtttctgt ttgctcggga ataacaactt agaaattgcc
    36781 cgaaacccca aaaacagaaa ccggacaaac aggttttccg ccaaagccgg cattttccga
    36841 ctttgcttca ggcatcttcc cctctgccta aaatcatcga cccgataccg gcatcggtaa
    36901 agatttccag caaaagcgcg ttgggcaacc tgccgtcgat gatgtgcgtg gctttcacac
    36961 cgttggcggc ggcttcgacc gcagaagcga ttttcggcag cataccgcca tacagcgtgc
    37021 cgtcggcaat cagcccatca atccgtttcg gcgtgagttt ggtcagcaga ttgcccgttt
    37081 tgtccatcac accggcgata ttcgtcatca tcaagagttt ttcggcgttc aattcttccg
    37141 ccaatttccc tgccaccaaa tcggcgttga tgttgaacgc ttcgcctttt tcacctacgc
    37201 cgacgggggc gacgacggga atgcagccgc gttctatcag ccctttaacc aaaccggtat
    37261 cgatgctttc caccgtaccg acctgtccga tgtccacgct attctgttcg ggcgtatcga
    37321 ccaaaagttt cttcgcctta atgaaatggt cgtcgcgccc gctcacgccg accgcgtgcc
    37381 ctccatatgt gttaatcatc gacacgattt ccttgttgac gtgcccgccc aataccattt
    37441 cgacaatatc catcgtctct ttgtcggtaa cgcgcattcc ttggacaaat tcgccctttt
    37501 tgccgacttt ttcaagcatc gcattgatct gcggcccgcc gccgtgaacg atgacgggat
    37561 gaatgccgac cagcttcagc agcacgacat cgcgggcaaa cccttctttc aaggcaggtt
    37621 cggtcatcgc gttgccgcca tacttgatga cggcgaccga accggaaaac cggcggatgt
    37681 aaggcagtgc ttcggcaagg atacgcgcct tgtcggcggc ggaaataatg ttttcaaact
    37741 ccataacggc tccatattgt tgacaatttc ggcttatgtt aacgccaaat gccgtctgaa
    37801 acaatcttca gacggcgttt ggcacacctt cgggcaggtt gaagccttgc gatacgggcg
    37861 gtaaaatcgc ggtttatccg gttcgagaaa ccggcgacag caaggacttt cccatgatta
    37921 aaatcagcac ccgcttcgat gccggctcgg tcgtcgtcaa agacctgacc gacccttcca
    37981 acatccgcct cgccctgcgt cccgacaacg cctccgattt cgcacaatgg ttctacttcc
    38041 ggctgcaagg cgcggcctat caaaactgca tcatgcattt tgaaaacgcg gcagaagccg
    38101 cctacccgaa aggctgggaa ggctatcagg cgtgtgcctc atacgaccgc cgcaactggt
    38161 tccgcgtacc gacttcctac gaaaacggcg tactgaccgt caatcatacg ccgctgtcca
    38221 acagcgtgta ttacgcctat ttcgaaccct attccgaaga gcagcacctc aacctcctcg
    38281 gcgacgcaca aggcagcggc ctgtgccgca tcgacgactt gggcagcacc gtgcaaggcc
    38341 gcgacatcaa tctgctgacc ataggcaacc aagtcgaaag cgacatgaaa atctggatta
    38401 ccgcccgcca gcacccggga gaaaccatgg cggaatggtt tgtcgaaggg ctgctcggca
    38461 ggctgctcga ttcgcaagac ccgacagcac gcaccctgct cgaccgcgcc acgttctaca
    38521 tcgtccccaa tatgaatccc gacggttcgg cactgggcaa ccttcgtacc aacgccgccg
    38581 gtgcgaacct caaccgcgaa tgggaaaacc cgactttgga aaaaagcccc gaagtgttct
    38641 tcgtgcgcgg aaaaatgctg gaaaccggcg tggatttgtt tttggacatc cacggcgatg
    38701 aaggtctgcc ctttgtcttt gttgcaggaa cggaaggtgt gccgaactac aatccgcgca
    38761 tttccgcgtt ggaagcgcag ttcaaaaccg cccttttgaa cgccagcccc gatttccaag
    38821 acgaatacgg ttacgaaaaa gacgcgccgg gcaaagcgaa tatgaccttg gcgaccaact
    38881 gggtcggcaa ccgtttcaac tgccttgcct acacgctgga aatgccgttc aaagacaacg
    38941 ccaacctgcc cgacgacgac ttcggctgga acggccagcg ttctttgcgg cttggggaag
    39001 cggtgctttc cgccattttg aacgtcgccg gcgatttgcg ctgacactcc ccactgaaaa
    39061 tgccgtctga aagccctttt gcgtctttca gacggcattt gaaaacgaaa gcccgttatg
    39121 attatccatc accaattcga tcccgtcctc atcagtatcg gcccgcttgc cgtgcgctgg
    39181 tatgccttaa gctacatcct cggatttatt ctttttacct ttctcggcag aaggcgcatc
    39241 gcgcaaggct tgtccgtttt taccaaagaa tcgctcgacg acttcctgac atggggcatt
    39301 ttgggcgtga ttttgggcgg acgcttgggc tatgtcctgt tttacaaatt ctccgactac
    39361 ctcgcccatc cgcttgatat tttcaaggta tgggaaggcg gaatgtcgtt ccacggcggc
    39421 tttttgggtg tagttattgc catatggttg ttcagccgca agcacggcat cggcttcctc
    39481 aaactgatgg acacggtcgc gccgctcgtt ccgctgggtc tcgcttcggg acgtatcggc
    39541 aactttatca acggcgaact ttggggacgc attaccgaca ttaacgcatt ttgggcaatg
    39601 ggcttcccgc aagcgcatta cgaagatgcc gaagccgccg cgcacaatcc gctttgggca
    39661 gaatggctgc aacaatacgg tatgctgccg cgtcatccct cgcagcttta tcagtttgcc
    39721 cttgaaggca tctgcctgtt cgccgtcgtt tggctgtttt ccaaaaaacc gcgcccgacc
    39781 gggcagactg ccgcgctttt tctcggcggc tacggcgtgt tccgctttat tgccgaattt
    39841 gcgcgccaac ccgacgacta tctcgggctg ctgaccttgg ggctgtcgat ggggcaatgg
    39901 ttgagcgtcc cgatgattgt tttgggtatc gtcggctttg tccggttcgg catgaaaaaa
    39961 cagcactgac cgcctgtgaa aaataccgtc tgaaacatgg aaatccggtt tcagacggca
    40021 tttttacgtt tggttcagat gaaaacaaac gcttcaattc ttaccgcaac acgccttgta
    40081 tttcctgccg ctgccgcacg gacagggatc gttcctgccg gttttttccc cttccctgcg
    40141 gacggtttgc ggtttgttga tgaccgcctg ccagtagcgg tagatgtctg ccagcgcgta
    40201 aggcagttcg gacgcaagtt ccgccagctc gccttcggtg aattgcaggc ggatagcgcc
    40261 gttttcctct tcgtcgtaaa tgccgcccaa tgccatcacg gggtaaaaca gctcttcaaa
    40321 cgcttcatca tcggcggctt caaaccaatc ggtcggcaca atgtccaaac cgtaaagata
    40381 ggcgttgcac caagtgtaaa aatcgctgcc gccctcgccg tcgtcgtaga gccacaaatc
    40441 gggcagcttt ttatccgaca tcgcggcggt tgtttccatc gccattgcca aaaccagccg
    40501 ttcgatttcg gaacgttcgg cggcggtaaa ttgcgattcg tcgcccaaca cttcgggcag
    40561 ccagtcgagc ggtgccaatt tgtccggccc gctcaacagc gccgtcataa aaccttgaac
    40621 ctcgtcgcaa cgcatcgtgt tgccttgttc gcttttggca tccaacaatt cgcccaaccg
    40681 ccgtttggat gcttcggtaa attttcggga atccatcatt ttccttttca aatgggtttt
    40741 gcgccctatt atagtggatt aaatttaaat caggacaagg cggcgagccg caggcagtac
    40801 aaatagtacg gcaaggcgag gcaacgccgt accggtttaa atttaatcca ctatatcgcc
    40861 gcaatgccgt ctgaagcctt tttatgcttc agacggcatc gtctgttttc acgctcaaat
    40921 cgtaccgctg ccctgcgcct tcggtttggc ggcactaaat tccagcttgc tcaaagcgga
    40981 aaggtaagct ttggcggtcg ccgccaaaat gtcggtatcc gcgccctgac cgttggcgac
    41041 gcggttgccg cgcgccaaac ggacgctggt ttcgccttgg ctttccgtac cttgcgtgac
    41101 ggcgttgacg gaataaatct gcaaagtcgc gccgctttgc gccacgctct caatcgcttt
    41161 gaaaattgca tcaacagggc cggagccggt tgcggaagcg cgtttttctt cgcctttgat
    41221 gccgaacacg atgtcggcgc gcggctcttc gccggtttcg gtgctgattt tttgagaaat
    41281 gaatttatag ctttcggcat tcatattgcc catttcgtcc gacaccagcg cgtgcaggtc
    41341 ttcatcgaag atttcgcgtt ttttgtcggc gagttctttg aagcgtgcaa atgccgcgtt
    41401 cagtgcctct tcgctttcca actcgatgcc caaatccgcc agcttggttt tgaaggcgtt
    41461 gcggccggac aatttgccca agctcaatcg gtttgccgac caaccgaccg attcggcaga
    41521 cataatctcg taagtttcgc ggtgtttcag caccccgtcc tgatggatgc ccgattcgtg
    41581 cgaaaaggca ttcgcgccga caatggcttt attgggctgc acgggatagc ccgtaatggt
    41641 ggacaccagt ttggacgaag gcacgatttg cgtggtatcg atgccggttt ccaagccgaa
    41701 caagtcgtgg cgcaccttca acgccatcac gatttcttca accgaagcat tccccgcgcg
    41761 ttcgcccaaa ccgttaacgg tacattccac ctgacgcgcg ccgcctttta atgcggcaag
    41821 cgaattggca accgccaagc ccaaatcgtt gtggcagtgt gccgaccaga cgactttgcc
    41881 gccgttgggc gttttggcaa tcagttcgcg gaaaaattct tcggttttgt acgggatgga
    41941 atagccgacg gtatcgggaa tattgatggt ggtcgcgccc gcttcaatca ccgcgccgca
    42001 gatttcggca aggaaatcga tttgcgaacg caacgcgtct tcgcaggaaa attccacatc
    42061 gtcggtgtat tcacgagcga tttttaccgc tttgaccgcc gcctcaatca cctgcttcgg
    42121 cttcatcttc agcttgtact ccatatggat ggggctggtg gcgatgaagg tgtggatgcg
    42181 ttttttcggc gcgggcgcaa cggccttacc cgcctgacgg atgtcccgct cgatggcgcg
    42241 ggacaatgaa cagaccgttg atttggtaat ggttttcgca atcgcattga ccgcctcgaa
    42301 atcgcccggg ctggcggcgg caaaacccgc ttcgatgatg tccgcgccca atttttccaa
    42361 ctggcgggca acgcggattt tttcctcttt ggtcatagcg gcgccgggcg attgttcgcc
    42421 gtcgcgcagg gtggtgtcga aaataataac gcggttggcc tgtgtcattt ctgttctctc
    42481 cagagattcg tttttttgta aaaaagcatt caaatccagc ggtcttcctt gcgattcggc
    42541 aagcgcggtc aatttttgca gttgcgccaa aggcagcgag ccgcgtgtgc gccatttgta
    42601 gatggcggcg gtcgtcgcgg cattttcagg atcgtgccgt ttcaacgctt cggcaagcgc
    42661 gttcacgccg ccgaaatagg cgaccagacg gtctatatct aattgcatgg tagtccttgt
    42721 ctaaaataaa tcagcccgca gcagaaatat ggacaggatt atacgcattt tggacaaaac
    42781 ggcaatatta atttgaaaaa atctcaataa gttgctataa attttattaa ttttatacat
    42841 tttgtctatt ttattgcaga tgcggcgtga ttgttgacaa ttgcgacaga acagggcaaa
    42901 ttcggcggta caaggcggca acgagacagg acaaaccaac aaggacggaa acaatgaaat
    42961 acaaaagaat cgtatttaaa gtcggcacat cttcgattac ccattcggac ggcagcctct
    43021 cgcgcggcaa aatccaaacc atcacccgcc agcttgccgc attgcatcat gcgggacacg
    43081 agctggtctt ggtgtcttcc ggcgcggttg ctgcagggtt cggcgcgctg ggtttcaaaa
    43141 aacgtccggt caaaatcgcc gacaaacagg cttccgccgc cgtcgggcag gggctgctga
    43201 tggaagaata tacggcaaac ctgtcttcag acggcatcgt gtccgcacaa atcctgctca
    43261 gccgtgccga ctttgccgac aaacgccgct accaaaatgc cggcggcgca ctttccgtgc
    43321 tgctgcaacg ccgcgcgatt cccatcatca atgaaaacga cacggtttcg gttgaggagt
    43381 tgaaaatcgg cgacaacgac acattgagtg cgcaagtggc ggcgatgata caggcagacc
    43441 tcttggtgct gctgaccgac atagacggtc tttacaccgg caacccgaac agcaatcccg
    43501 atgccgtacg gctggacaaa atcgaacaca tcaaccatga aatcatcgaa atggcgggcg
    43561 gctcgggttc ggcaaacggc acgggcggta tgctgaccaa aatcaaagcg gcaaccatcg
    43621 ccgccgaatc cggcgtaccg gtgtatatct gttcctcact caaacccgat tcattggccg
    43681 aagccgccga acatcaggcg gacggctcgt ttttcgtccc ccgtgccaaa ggtttgcgga
    43741 cacagaagca atggctggcg ttctattccg aaagccgggg cagcgtttat gtggacgaag
    43801 gtgcggaaca cgctttgtcc gaacaaggga aaagcctgct gatgtcgggc attgccggaa
    43861 tcgaagggca tttttcccgt atggacaccg taaccgtgta cagcaaggca accaaacagc
    43921 ccctgggcaa agggcgcgtc cttttcggct ccgccgccgc cgaagacctg ctcaaatcgc
    43981 gtaaggcgaa aggcgtgttc atccatcggg acgattggat ttccatcacg cccgaaatac
    44041 gcctgcttct gaccgaattt tagaaaagga aacccatgtc aaacacacaa aaacagcttg
    44101 cccttgccaa agcggcaaaa aaaatccgtc aacacggcgg atgcggaaga aaaaaaccgc
    44161 gcgctgcttg ctatggcgga cagtctggaa gcggcggcgg aagatatttt ggcggcaaac
    44221 cgtttggatt tgaaagcggc ggcaggcaaa attcccgaca gcatgaccga ccgccttttg
    44281 ttggacggca aacgcatttg cgcgatggcg gacggcatca gggcggttgc cgcgctgccc
    44341 gaccccgtgg gcgaaatact ggaaacctcg actctgccca acggcttgga aatcgtcaaa
    44401 aagcgcgtgg cgatgggcgt tatcggcatt atttacgaaa gccgcccgaa cgttacttcc
    44461 gatgcggcgg ctttggcact gaaaagcggc agcgcggtcg tactccgcag cggcaaagat
    44521 gcatttcaat ccgcacgcgc catcgttgcc gccctgaaaa cggggttggc acaaacccgc
    44581 atcgaccccg aagccgtaca gctcatcgaa gacacagggc gtgagggcag ttacgaaatg
    44641 atgagggcga aagattatct agacctgctg attccgcgcg gcggggcagg gctgatacgg
    44701 gcggtggttg aaaatgccgt cgtgccggtc attgaaacgg ggacgggcat cgtccacatt
    44761 tatatcgaca aggacgcgga ttgggacaag gcactccgta tcgtgtacaa cgccaaaacc
    44821 ggccgcccgt ccgtgtgcaa ctcgatggaa gtgctgctgg tgcatgaagg cattgctgcc
    44881 gacttcctgc ccaagctcga acggctgttg gttcgcggcc gtatagaagc cggactgccg
    44941 cccgtccgct tccgtttgga tccgcaggcg gcgcggtata tcggcggcga agcggcgggt
    45001 gcagacgatt tcgataccga gtttttagac tacatcctcg ccgtgaaaac cgtcgcttcg
    45061 gttgaagagg cggtcgggca catcgaagcc cgcggcacgc accattcaga cggcatcgtt
    45121 accgaaaacc gccacgctgc ggactatttc acgacccata tcgattctgc cgccgtgtat
    45181 atcaacgcgt ccacgcgctt caccgacggc ggcgaattcg gcttgggttg cgaaatgggc
    45241 atctccacgc aaaaactcca cgcccggggc ccgatgggtt tgaaagagct gacgagttac
    45301 aaatacatcg tacaaggcac gggacaggtt agggaataat ccgaattcaa acgtctgata
    45361 tagtggatta aatttaaacc ggtacggtgt tgcctcgcct tgccgtacta tttgtactgt
    45421 ctgcggcttc gccgccttgt cctgattttt gttaatccac tataaaaatg ccgtctgaaa
    45481 cagtcttcag acggcatttc tatttgcaaa aaaatcaagc attgtccaag gggacgagaa
    45541 tcgtacggtt gccgttgtgt tccggtgcgg acacaatgcc ttccgcctcc atctggtcaa
    45601 tcagacgcgc ggcgcggttg tagccgatgc gcaaggcgcg ctgtacgccc gaaatgctgg
    45661 ctttgcgcgt tttcaggaca acggatacag cctcgtcgta catcggatcg gtttcgccgt
    45721 cgccgctgcg cccgataccg ggcagctctt cgctgccgcc gccgctcaaa atatcgtcaa
    45781 catagtccgg ctcgccaaac tgtttcaaat attcgaccac gcggtgcacc tcttcatccg
    45841 aggcaaacgc gccgtgaacg cgttgcggat aggcagtacc cggcggcagg aacagcatat
    45901 cgccctgacc gagcaggttt tccgcgccca tttggtcgag aatcgtgcgg ctgtcgattt
    45961 tgctggacac ttggaacgcg atacgcgtcg ggatgttcgc cttaatcaga cccgtgatga
    46021 catcgacgct ggggcgttgt gtggcaagga taaggtggat gcctgccgcg cgggcttttt
    46081 gggcgaggcg cgcaatcagt tcttcgattt tcttgcctgc cgtcatcatc aaatcggcaa
    46141 actcatcgac cacgaccacg ataaacggca gtttttccaa aggttcggga tcgtcgggcg
    46201 tgaggctgaa cggattgccg attttttctc cccttgctgc ggcttcggcg attttttggt
    46261 tgaagcccgc aagattgcgc acgcccataa agctcatcag gcggtagcgt ttttccattt
    46321 cgttaacaca ccagttcagc gcgtttgccg ccagcttcat atcggtaacg acaggggcga
    46381 gcaggtgcgt gatgccttcg taaatgctca attccagcat tttcggatcg atcataatca
    46441 tacgcacgtc ttccggcgcg gctttgaaaa gcatggacag aatcatcgcg ttgacaccca
    46501 ccgatttgcc cgaaccggtc gtgccggcaa ccagcaaatg cggtgctttg cccaagtcgg
    46561 ttacgacggg ctgtccggtg atgtcctgac cgagcgcgag cgtcagcttg gatttggatt
    46621 cggcaaactc gggcgaattg aaaatttcgc tcaggcgtat catttggcgt ttcgggttcg
    46681 gaagttccaa acccatgcag gttttgccgg ggatggtttc gacaacgcgg atggaagcca
    46741 cgccgagcga acgcgccaag tctttttcca aattcagaac ggaattgccg cgcacgccga
    46801 catcgggttc gatttcataa cgcgtaatca cggggccgga ataagaatcg acaaccttga
    46861 ccttgacttt gaactccgcc aatttttctt cgatggtgat gctgttttcc aacagttctt
    46921 cttcggtttg cgtcgcctcg ggattgaaca gcggaggcag aagcaggtcg gttgtcggca
    46981 ggtgttcgga taccgcaccg gtctcttccg attggaacgc cccttcatcc gcttccgtat
    47041 ccgatacccg gcaggacggg cgttcagacg gcacatcttc aaacggacaa accgcctgac
    47101 tgtcatgccc gaacgcttcg gtttccgaca gatattgccc gcttgaccgc tctgccgcac
    47161 cctcactgcc gtcatctgca atagcggcgg tttcctcctg ccaacctcca ttcaaaacat
    47221 cagcggcaag atggtcggtt tcggcaatgc ggctgcgttg cgcctgctcg aatcctgccg
    47281 gcggctcata ggtacggttg tagatttccg atacgggagg cggcggcaga atatcgattt
    47341 cgggtacggc tacctccggc acttcgggtg gttcgaccac ccaagcatcg ggcgcggcag
    47401 gctcttcaat atggatatcg gcagcttcat cataaaccgg accgccgtat cccacagacg
    47461 aaacggtttc cgtgaaaaca ttttcggttt cagacggcat ttcgacatcc cgtttcccga
    47521 caaccgtccg actttccgga atcaggcgcg aaatccgcgc ctccgcagaa accgttgcct
    47581 tgttcggttc tttcaaattg acggaaacgc ggcggcgggc atcgcggacg gctgtgccgt
    47641 ctgaaacgga ctgcccttgg gaaacatccg gggattttgc ctccgcctgc cctttctcct
    47701 gccctgcatg acggcggaac ggcgcattcc gtgcattttc ggctttgact gcagaaaagg
    47761 cagacggatg ctgcttctca aaatacggct caaatccgta atccgcagac tcggaaaacg
    47821 cttctttgtc cgcatcaaac atacgggaat acgtgcgttg aagcacaggg tcgtccaaac
    47881 cgataatctg cagcccttcc atcggggtat cggacacgcg tactttgggg acggctgttc
    47941 cgtttttctc aaatgcatcg atatagcgtt ttttcgtttc ccttaaagcc gcgctgctta
    48001 aagcacgcgt tgcttcttcc aaagtgattt ccttaaaaac cggacggacg ggcgaagttt
    48061 tgctttcaga ctgcatcaac ccttcagcaa tactccggtc gaatgggatg cggcggttgt
    48121 cgattacggc agttgcaatg tcttccgtat cggcagcctc ttcctccgca gcttctgctg
    48181 cttccgtttc cgcttcttcc gtcccgtttc cactgtctga atacccgtcg gatgcggtcg
    48241 gaacatccgc acggtttgca gaagcaggcg gaacgggcct gtccggttcg gattcgtgcg
    48301 ccagatagtt tcggcagaaa cggacggcac cgacaaacag ccaatacact gccgttttga
    48361 cggcatggaa aagcatcagg gaaaactcgg gaaaatcggg cataccgtct ttgatttcag
    48421 gcagctccgc ctgtttttcc cctttctttt cctgccacgc agaaacctcg cgcatccact
    48481 cgcgttcgga ttgtgcgcgg acaaaaaaca ggccggcaag cgcaagcaac aaaataacga
    48541 tcaaaactat ccaaaacata tgccgtttcc cgcaagacaa acgattaacc gagtttattt
    48601 taacgtgttt ccagcccaaa aaaaacaacc ggccggcaat tctgccgaaa ccgattcggc
    48661 tttcggtttg cccccgccga cagtataata gccgcccctt cccttaatct ttgcggcatt
    48721 accatgattt tccaaacagt ctggttttca gatgtggtac tgtcggtttc gtggattgtc
    48781 cttatcctga tactggcagc ttccgcgccg tcggcattcc gttcgcttgc ccgctaccgt
    48841 tccgcccttc ccctgtgtac cgtaattttt tccgcagcat ggtgtctgaa cgcctctgtc
    48901 ggcggcggac agcttgccca gatgaactac cacctgcttg ccgtcaatct ggttacactg
    48961 atggtggata cctccgccgc cctttggctt gccgcgctgc tgatgctgcc ctactgcctg
    49021 ctgtttgccg gttctgccgg cgcatatccg ccgaacgctt tggtgctgat actgcccgcg
    49081 cttgtcgtca accgcctgtc gcgtatgctg gtcaaccgtc tgccgcccaa tattttcatc
    49141 tttattttcg tcaacggctt tctcgcttcc gccgccggca ttctgctgac cgggctggtg
    49201 ctgatcggta ttttggatgc cgcaaacgct tttccgtccg aaatattgtg gacgaccgcc
    49261 ctgcccgtct ttattctgct ggcgtgggcg gaagccttcc tcagcggcat ttcaactgcc
    49321 attttcgtgg cactgaagcc ccattggatc aacactttcg acgacaaccg ctatctgaag
    49381 tccgagcgcg gcatatggct gtagccttca gacggcatag gcagacaaaa ccatgctttc
    49441 caacatcctc cccctttcca tcggtgcaat attcggcaca acggcgcgtt ggctgctcaa
    49501 cctcgccgtc cccgcatcgt tgtctcccgc cacaggcaac ctgtttgcca actggacggg
    49561 cgcgctgctc atcggcatct ttgccgaaac cgtcagccat ccgcaatgga agctgctgct
    49621 gattaccggt tttttcggca gcctgaccac gctttccgga ttttcactgg aaaccgtaac
    49681 cctgctccaa tcgaaccgtc ccgcttcggc acttgccaat attttcctac acacggcagg
    49741 ttcgctgctg ctgacttggc tcgggctgaa gatagggacg gcagtcaaat aaccgcaaaa
    49801 aattgcttcc tttggttttg acataatcta taaattggct tcgcttgttt aaatactgcc
    49861 tgtgccaatt atagtggatt aaatttaaat caggacaagg cggcgagccg cagacagtac
    49921 agatagtacg gcaaggcgag gcaacgccgt accggtttaa atttaatcca ctataccgtt
    49981 ctcaaattca aattgggctt ttaggtctat aacaaagaaa aagccttggg aggataaacc
    50041 taccaaggct ttttcttaaa aaacgctcta tcactgtgcc taaaaatgca aaaaagtaac
    50101 cgtcataatc cttttgccaa aatcaatatc atcatccgat gaatgaacgg acggtatata
    50161 agaaagcgtt aataaactca taaaccagta agaaaaatca gtaacgaaga tattcattat
    50221 tacccattct taaaaattat cacgttcgtt tgggtaatta tcataccctt ttccctctta
    50281 ttactaaagc acgaattact ttaaagtacg cacccagaga caagccgaaa aggcatatcc
    50341 gatccgccaa acaaaatgcc cgccgactta ctccggcggg cggttccaac atcctgtcaa
    50401 tccttcatca gccagtattg caaaccgatt aatgttttac cgtctttaat ttcatcgttt
    50461 gccaatgcct gacggacttc atctttcgac atcagcacgg tttccgttat ttcatcttca
    50521 tcattagaaa gcgtactgcc caagcgcacg ccttccgcct cgaacagata cattttttca
    50581 ttgcaaaaac cgaccgccgt ataaaaactg tacagcaggc gcacgctgtc ggcaacataa
    50641 ggcgtttcct ccgccaattc tcgcagcgca cacgctgcca tatcctcgcc cgccacatcc
    50701 agcttgcccg caggaagttc caatgtcgcc tgatttgccg cataacgcca ctgccgcacc
    50761 aaaacgacct tttcgtcatc cgttaccgcc aacacgcacg ccgcgcccgg atgccggatg
    50821 acgatgcgct gcccttcatt gccgttgggc agcctgacct tatccctgct gatactgacg
    50881 aaaccgccct cgtaaatggt ttcgccgcct aattttactt ccctcaaatc cacttcatat
    50941 ctcctttccg tttcctattc tgccgttcaa tcttcacgct tgccgcgctc gatttccact
    51001 ccaacctcgc gcacgccttc caaaataccc ggcttgacga ttttcacgtg cacccacacc
    51061 gcgccgaaat atcccaaaac caaatcggca atatattccg ccaacgcttc caaaagcagg
    51121 aaatcctgtt ctttcagatg tcggcgcagc gtttcgcata cctcggcata atgcaccgta
    51181 ttggcaatat cgtcgtccga acccgctttc tcgggaacgc cgatgtccaa atcgacaatc
    51241 agggtctgca accgttcgcg ttcccagccg tacacgccga taagcgtatc cgccttcatg
    51301 ccgtgcaaaa agattttatc cataatccgc cccgtttttt gctaaaataa ccgcaccatt
    51361 ctaagtaaag taccgcaaat gttcaacata ccggccgtcg ccgtttccta tttaatcggt
    51421 tcactttctt tcgccgtcat cgtttccaag tattacggga tggacgaccc gcgcacctac
    51481 ggatcgggca atcccggcgc gaccaatgtt ttacgcagcg gcaaaaaaaa ggcggccgcg
    51541 ctgacgctct tgggcgatgc cgccaaaggt ttggttgccg ttttgcttgc acgcgtgctt
    51601 caagaaccgc tcggtttatc cgacagcgca atcgccgccg tcgcactcgc cgcgctggtc
    51661 gggcatatgt ggccggtgtt tttcggattt aagggcggca aaggcgtggc aacggcattg
    51721 ggcgtgcttc tggcactctc tcctgcaact gccttggtct gcgcgttgat ttggcttgtg
    51781 atggcattcg gcttcaaagt atcctccctt gccgcgctgg tcgccacaac cgccgccccc
    51841 cttgccgcac tgttttttat gccgcatact tcttggattt tcgcaaccct cgcaatcgcc
    51901 atattggtgt tgctccgcca taagagcaac atcctcaacc tgattaaagg caaagaaagc
    51961 aaaatcggcg aaaaacgctg attcggcatt tgttttccat tacgtcatgc cgtctgaaac
    52021 catgttcccg cgttcagacg gcatttccgc acaaacggct ttcatacata ccatgctcag
    52081 ttacagacac gcatttcacg ccggcaacca cgccgatatg ctcaaacact tcaccttgtt
    52141 tttagtgctg caatatttca accgcaaaga caagccctac tggtacatcg acacgcacgg
    52201 cggcgcgggt ttgtacaatc tcgaaggcag cgaggcgcag aaagtcggcg aataccggca
    52261 aggcatcgcc ctgctccgac aggcgcaaaa cctgcctgcc gaactctctg actttgccgc
    52321 gcacatacaa aaaatcctac cctcgcccga actttactgc ggctctccgt ggctggcgca
    52381 atcgctgatc cgcatcggcg acaaattgcg cctgtttgag ctgcaccctg ccgactttgt
    52441 ccatctgcaa aacaatatgg gcgaagcggg attgggcaaa cgcggacaag tgttgcgcga
    52501 agacggttac aaagggctga tttccctgct gccgcccccg ccgcgacgcg ctgttattct
    52561 catcgacccg ccctatgagg aaaaacagga ctaccggcgt gtaacggaaa cgctgaaagc
    52621 cgctttaaaa cggtttgagt ccggctgtta tctcatttgg tatccctgcc tcagccgcga
    52681 agaaagccgc aagctgccgg aagaattgaa aaaactcctg cccgacaact acctttacgc
    52741 cgaactgcac gtccacgcgc cgaaaaccga cggtttcggt atgcacggca gcggaatgtt
    52801 cgtgattaat cccccttacc ttttagccga acaattggcg gcaaacctcc ccgccctgac
    52861 acggctgctg gcgcaagacg aaggcgcgcg ctacctgttg gacaacaaaa tccgctgacc
    52921 ccgatgccgt ctgaaaccct tcagacggca tcttccgtca aacaaacaaa accgggcgat
    52981 acacactcca tatcgtccgg ttctgcataa aaccagtatt caaaaatcag aacacgcttt
    53041 tcattacacc cacggtacca accacaccgt tcagcagcat cagaccaccg gcagccgtac
    53101 ccataccttg acggattgcg tcatgcttat tggcacgttt ttcacgttcc aaagccaact
    53161 cctcagaaac atttttacgt tgacggcggt gttgcgccag ttccgcacgg ctaattcgcg
    53221 cttcggcacg gttacgacca gccttgatta cggccgaatc gtcgccgata gcatagttgg
    53281 ctacagaatt ttcagaagcg caggcagcaa gcaaaacggc acacacagca acaacggaaa
    53341 tatttttcaa gtttttcata cctgtcctat tatctcaaaa taaagaaaaa actaatccaa
    53401 ctggagtttt atcggataaa ataaatattg ggtgaatttt cgtttgcctt tttgatggcg
    53461 cgtgccgcat ccatcatttc ttcccggcgt tctttacggt attcggcacg ctcggcacgc
    53521 tcgcttatct gaatggcacg acttttcgcc tgtatttccg ctgcagattc ttgagttaca
    53581 aaacggcggt ctgcgtaagt acatgccgtc accaacatcg cagccaccga caacaaaatc
    53641 agtttcttca ttttcatcta cctatattta agtttatttg taaaatatgt tatcactata
    53701 tatatatata atgtcaatat tttttaaaaa taaatttggc atcttgtact tctcgatccg
    53761 cctgccgcgt tccgacttgc cccgcgccgt ttttttccac tacaatccca cgcgatatcc
    53821 ggcgaaacgc accaaaggaa cgccccgcag caatctgccg cctttcagac ggcacaaacc
    53881 tttccgaaac gagcaaacca tggacacact gacccgattc ctccccgaac acctgcaaca
    53941 aaaccaactg cccgaagcac tcggcggcgt tttgctgtcc gtcgtttccg cctgtaccga
    54001 aatcaacgcc aaagtccgcc tcggcgcact tgccggcgta ttgggtatgg cgggcacggg
    54061 caatattcag ggtgaagacc agaaaaaact ggatgttatc gccaacaaca ttatgattga
    54121 cacactcaaa gccaaccctg ccgttgccgg tttggcaagc gaggaagaag acactttcgt
    54181 aagtgccggt gaaaacggac gctatctcgt cctattcgac cctttagacg gatcgtccaa
    54241 tattgatgtc aacatttccg tcggtacgat tttctccatt ctcgccaaac ccgaaggcgc
    54301 attggcaacc gaatcattcc ttcaaacggg cagacagcag cttgccgcag gctatgtcct
    54361 ctacgggcct caaacccagc tcgtattcac attcggacac ggcgtgtacg tatttacact
    54421 caatgccgaa aacgaatttg tgctgaccaa agaaaacccg aaagtacccg aaagtaccaa
    54481 agaattcgcc atcaatatgt ctaaccgccg ccactggcta ccccccgttc aacaatacgt
    54541 cgacgagctc ttggcgggcg aaaccggtac gcgcggcaaa aactacaata tgcgctgggt
    54601 agccagtatg gttgccgaaa tccaccgcat cctgatgcgc ggcggcgtgt tcatgtatct
    54661 gcaagacaaa cgcgacccgt ccaaacccgg caaactgcgc ctgatgtacg aagccaaccc
    54721 tatggccctg atactcgaac aggcgggcgc atcggcaagc aacgcatatc aagccatgct
    54781 cgatatacag cccgaaagcc tgcaccaacg cgtcgccgtc attatgggca gcagcgaaga
    54841 agtggattac ctcaaccgtc tgcattcaaa ataggcagag accgtactga taaagaccgg
    54901 cggcagcgtg aatccgatac tgcgccgata tttcaagcat accctccgga aaaacagaaa
    54961 actgcctttc cgaacgataa aaataccgtc tgaaaaattt tcagacggta ttttaaaaat
    55021 taaagctcaa atttatttgc ccgccgcttt ttgcaggatg gcgtacaact cgtctttcag
    55081 tttcaatttg gcttttttca gctcatcgat ggtttccgca ccgctggtaa ccggattgtt
    55141 ggccagaccg gtaattttat cgtccagctc gttatgctcg tcgaacagac gggcgaagcg
    55201 ggaattttcc tgtttcaatt tggaaatcaa atcacggtat tctgggaaca taatcgcttt
    55261 cctctcttgg tttacggtta aaaagtcaaa ttgaatcttt acattaatta taacaaagat
    55321 aatattttta tccagtgttt tgttatacac ggcaacaccg tccgcagaaa accgccccac
    55381 cgtccgataa aatcgttgac aatcttccgc tttacgcata aattttcagc ccttcctact
    55441 gcatatgaaa ggcacgcccc agtttcccca atgtggattt tcagcgcgcg cagtagaggt
    55501 gccgacccaa attggcagac cctttgcttt tgttaatatc ctagaaaatc cagaaattcg
    55561 cagtactcta ccactcatcg ccaattggcc aacctttcca cggctttggg ttaatggtga
    55621 gttaatcggt ggtagtgata ttatcctaca aatgtaccaa tcaggtgagc taaaaccact
    55681 cattgaagag catagcccaa aggtctgatt acttaatatc ttaaaataaa tatcaaaaaa
    55741 gactgctgat gacattagcg gtcttttttt gatggtcaag attgatagtt tggattgaat
    55801 tatttagcca tattacatac aataatcagc atgctttgta tgaattatca atcaaactca
    55861 ggcgaaggag tgcttgtggc taaaacatat ttattgactg cattgataat gtctatggta
    55921 atctccggat gtcaagtcat ccatgccaat caaggtaagg ttaatactaa ttctgctgtc
    55981 atcgcaggtg cagacgctca cacgcctgaa catgtaacgg gactgaccga acaaaagcag
    56041 gtgattgcaa gtgattttat agtagcgtca gccaatccat tagcaacaca agctggctat
    56101 gatatcttaa agcaaggcgg tagcgctgca gatgcgatgg tggcggtgca gacgacacta
    56161 agcttggtag agccacagtc gtcaggcttg ggcggtggtg catttgtgtt gtattgggac
    56221 aataccgcca aaacattgac cacatttgat gggcgtgaga cggcaccgat gcgtgcgacg
    56281 ccagaattat ttttggataa agatggttaa ccattgaaat ttatggaagc ggtggtcgct
    56341 cggtaggtac gcctgctatc cctaaactga cggagacaat acatcagcga tacggtgtat
    56401 tgccttgggg aaaattattt gatacgccga tccacttggc aaaacaaggc tttgaggtgt
    56461 cgccaaggct tgccatctcg gttgagcaaa atcagcagca tttggcacgc tatccaaaaa
    56521 cagccgctta ttttttgccg aatggtgtgc cgctacaagc aggcagcttg ctgaaaaatt
    56581 tagaatttgc tgacagtgtt caggcgttag cagctcaagg tgcaaaagct ctgcatactg
    56641 gtaaatatgc ccaaaatatt gtttcagttg tccaaaatgc taaggataac cccggtcaat
    56701 tatccttgca aaatttatct gattatcaag tggtggaacg cccgcctgtt tgtgtgactt
    56761 atcgtattta tgaagtatgc ggtatgggtg caccaagctc aggtgggatt gctgtgggtc
    56821 agattttggg gattttaaat gaattttcac ccaatcgggt ggggtatgat gctgaaggtt
    56881 tgcgtctctt gggcgatgct tctaggcttg cgtttgcgga tcgtgatgta tatttgggcg
    56941 accctgattt tgtaccagta cccattcgcc agttgatttc taaagactac ctaaaacatc
    57001 gcagccagct gcttgagcag tcggataagg cattgcctag cgtatctgca ggcgatttta
    57061 ttcatgagtg ggcgtcctca caagcgattg agttaccttc taccagtcat atatcaattg
    57121 tggataaggc gggcaatgtg ctatcaatga cgacttccat tgaaaatgcg tttggctcaa
    57181 ctttgatggc aaatggctac ctgctgaata atgagctgac agactttagt tttgagccaa
    57241 taaagcaggg caaacaggtc gccaatcgtg tcgagcctgg caagagacca cgctcctcaa
    57301 tgacaccaac catcgtattt aaagcaggca agccttatat ggcaatcggc tctccaggtg
    57361 gtagccgtat cattggctat gtcgctaaga cgattgtggc acatagtgac tggaatatgg
    57421 atatccaaga tgccatcagt gcacccaatt tattaaatcg ctttggtagc tatgaactag
    57481 aaacgggtac aaccgccgtc cagtggcaac aaacattaaa tgatttgggc tataagactg
    57541 atgtgcgtga gctaaattca ggtgttcaag ccattattat cgagccaagc aggctggttg
    57601 gtggtgtcga tccacgccga gaaggtcgag tgacgggtga ttagaggtga agcttgtatt
    57661 ttgtgaattt gaccgcatta agttaattat taaaataggg ggaaattgtg agtaatcaaa
    57721 caaaaataca agccatcaag caagcttctg agcagatctt gacaatttgt gagacgccaa
    57781 acacagcatt acaagccatt catctgattt taagacatgg cggggcagga agaattgtct
    57841 tggcaagtgg tgtatcagcg ggttatggct gataaagatg tggttggtgc tggttatttg
    57901 attgattttg cacaaactgc cgaaaattta ccatttgatg tattgccttt gattagcctt
    57961 gtgctaaaca aaggtgatga gacgctaaaa accggcatgt taaataaatt acctgataat
    58021 gccaaagaaa atttacgcat tatgggctat ctgccttagg ttttgaagta tttttttata
    58081 cgaaaaacgc tcatgatacc ctgattacac aaaggagccg agaatatgtt ttcaaaaagc
    58141 gttaccctcg cacaatatga ccccgatttg gcagcagcca ttgcccaaga agaccgacgc
    58201 cagcaagacc acgtcgagct gattgcctct gaaaactacg tcagctgcgc cgtgatggag
    58261 gcacaaggtt cgcaattgac caacaaatac gccgaaggct atcccgcaaa acgctactac
    58321 ggcggctgcg aatacgtcga tattgtcgaa caattggcaa tcgaccgcgt aaaagaactg
    58381 tttggcgcag cctatgccaa cgttcaaccg cactccggtt cccaagccaa ccaagccgta
    58441 tatgcttccg ttttaaaacc gggcgacacc attttgggta tgtctctggc gcacggcgga
    58501 cacctgaccc acggcgcgag cgttaatatt tccggcaaac tctacaatgc cgttacttat
    58561 ggcttggatg aaaacgaagt cctcgattat gccgaagtcg aacgcctcgc gctcgaacac
    58621 aaacccaaaa tgattgtggc gggcgcgtct gcctacgcgt tgcaaatcga ctgggcaaaa
    58681 ttccgcgaaa tcgccgataa agtcggcgca tacctctttg tcgacatggc gcactatgcc
    58741 ggactggttg ccggcggcga atatcctaac cccgtgccat tctgcgactt cgtaaccact
    58801 accacccaca aaaccctacg cggccctcgc ggcggtgtga ttttgtgccg tgacaatacc
    58861 cacgaaaaag cgctgaactc ttccatcttc ccaagcctgc aaggcggtcc gttgatgcac
    58921 gttatcgccg ccaaagccgt agcgtttaaa gaagcgttgc aacccgagtt caaacaatac
    58981 gcaaaacaag tgaaaatcaa tgctgccgtc atggcggaag agttggttaa acgcggtttg
    59041 cgcatcgttt ccggccgcac cgaaagccac gttttcctcg tcgacctgca accgatgaaa
    59101 atcaccggca aagccgccga agccgctttg ggcaaagcgc acatcaccgt caacaaaaac
    59161 gccatcccga acgatccgga aaaaccgttc gttacctccg gcatccgcat cggttccgcc
    59221 gccatgacca ctcgcggctt taacgaaacc gacgctcgcg tattgtcgaa cttggttgcc
    59281 gatgtattgg ctaatcccga agacgaagcc aacctcgcca aagtccgcgg acaagtgact
    59341 gccttgtgcg acaaataccc cgtttacgga aactaaacgc ccgatgtttc catacaaaat
    59401 gccgtctgaa cattttccag acggcatttt ttgttttcgg taacattgct attttttctc
    59461 ttccagcaaa atgaccaact ttttcaatac tgcgatttca ctatctttct gcctgatcaa
    59521 ttcatccttt gcatccagca gcgacctcaa tttttcattt tcaaacacca gcatttcatc
    59581 agagccgata atattatgat ggttattggt attgtcgccg atagagaaga aaaacgatcg
    59641 gttctgctcc gccagcagct caaccacatc gatattgaaa atctgggcaa tctggcgcag
    59701 cttgtcgaga ttgatgccgc ttttcccgcg ttctattttc gaatacccgt taaccgacat
    59761 ttccaatttt tccgccatct cttcctgcgt ccactgattg acttccctta acgtccgaat
    59821 cttgtcatgc acttccatcc tgccctccaa ggttaatttt caccactcaa acaaccgtaa
    59881 ccggtatctt cagttgatgg tcggatattt taaaaataaa taatataaca caatagtatt
    59941 ttgataaaaa tttacctaac gaggtacaga catgtattca gaaaacaagc ctaaacgcac
    60001 cgtaaaaatg aaaaaatact ggcagcctgc accctttccc tgtccctttc tgcctgtact
    60061 tcgactcaag taacttcttt tgtcgaggga ctctccgagg gactcgtaga tgtcagcgaa
    60121 gcctttatca caacaaaggc agaatcatat tctgccccgt ctcttcagga taagcaaatt
    60181 ttcataaatc gcgtctcaga acgagcacaa aaaaattaca aagaaagaag gaaaaagaaa
    60241 gaagcagact tactaaattc agcatcgtca ttaaatgaat caacatcaga agccctatct
    60301 gcccttaaaa acgctatcgt aattcctaaa cgccctaaac gctccttggg caaaaaatat
    60361 aaaaacggtt ttcatcctgt aaatacgtgt gcggatgtgg aagctcggtg ggcaaacgga
    60421 gcaatgagag atgttaggga catagaaagg ctaataagcc gaaacgtccc tttacgtcgt
    60481 atcggagacg ccattcaaaa catgtgcatg gaaaccaatg tgtccattag gatgtatcgc
    60541 caggagtgcc gttcagaatc gtcagatttt tatgacgaaa tgcttgagca tcacgagaat
    60601 ttcataggat cctccaaatc attcgcatca tcacttcttc cccttagcgt tacaaacttt
    60661 acaacagaca tggcgtgtac tcccgaattt taatctaatc acactccaca actccctatc
    60721 aatataactg ctacttctct gagtagtatc aaccaaccct taacaaagga ctaaaaaatg
    60781 aaaaaagcac tgcttgcact gactattgcc gccatctccg gtactgctat ggcccagttg
    60841 cccgactttc tgggtaaagg cgaatatacc gtccgtacag acatctccaa acaaacgctg
    60901 aaaaatgccg atttgaaaga aaaacacaaa gtacaaaaaa acatcggttt ccgtgccgat
    60961 atgccgtttg acgatattca ccacggcatg cgtttcgaag tatcacacag ccgagacaaa
    61021 aaagacatgt acgttgtgac cgaaagtact actaaaccat tcggcaaaga cgttaaagaa
    61081 aaacgcaccg acgtgtatgc cggttacacc tacacccaac cgctcagtga agccaccaaa
    61141 ctgcgtgccg gtttaggttt gggctatgaa aaatacaaag atgcggtagc taatgagaaa
    61201 ggaacagtca gcaccgagcg cgaagccttc tataccaaag cgcacgccga cctgacttcc
    61261 gatttgggcg gcggctggta tctgaatcct tgggcagagg ttaaggttga cctggacgcc
    61321 aaattgaaac acaacgcaac tgtagccggt gcctcagccg acattaacgc aaaaacacgc
    61381 ggatggggcg tgggcgtagg tgcgaatatc ggcaaacaaa ttaccgatac cgtcggcatc
    61441 gaagccggcc cgttctacaa acaccgtcac ttcaaagcat ccggcagctt cgttttagac
    61501 ggcgggaaca tccgagtcga tcctaccaaa atcaacgaat acggtgttcg tgtcggcgtg
    61561 aaattctaat cgcggcgata tgccccagac acaaaaccgc aagccttttg gtttgcggtt
    61621 tttgatgttg tagcagaccc taataccgtc tgaaactttt cagacggcat ttttccaatc
    61681 ggttaaaata ccgcctcgac attgtgtttt ggaaaacccg tgcttgcttc tgtgattgat
    61741 tttatcctgc atatcgacca acacctgctc agcctgtcgg cgcaatacgg tgtgtggatt
    61801 tatgcgattc tgtttttgat tgttttttgc gaaaccggtc tgattgttac gcccttcctg
    61861 cccggcgatt cgctcttgtt tgccgccggc gggattgccg cgctgggcgg tatggatatt
    61921 catttgatgg ttgcattatt aagccttgcc gccatactcg gcgacgcgct caattttacc
    61981 gtcggcaaat atttcggcgg caggctgttc gccaaccccg attctaaaat cttccggtgc
    62041 gaatacctcg gcaaaacccg ccgtttttac gaaaaacacg gcggcaaaac gattatcatc
    62101 gcgcgcttcg tgcccatcgt ccgcaccttc gcccccttcg tcgccggtat gggaaaaatg
    62161 cactatgcca aatttatccg ctacaacatc atcggcggct tgttgtgggt tatcttgttt
    62221 tcttacgcgg gctatttctt tgccaacttc cccgtcgtaa aaaacaatct gggcttggtg
    62281 atgggcggca tcattattgt ttccgtcctg cccggcatca tcgaaatcgc ccgcgccaaa
    62341 cttgcggcaa aatccgaacg ttaattgttg gaaattgaca ttatgaatat attatccgta
    62401 gaaaacgctt cctttgccgt cggacatgtc gccctgctcg acaaaacgtc cttccaactc
    62461 gacagcggcg aaaaagtcgg cttgatcgga cgcaacggcg caggcaagtc ttcgttttta
    62521 aaaatcctca ccggcgtgca aaaactcgac gacgggcaga ttattgttca aaacaatctc
    62581 aaaatcgttt atgtgccgca ggagtccttt tttgataagg acgcaaccgt atttgatact
    62641 gttgccgaag gtttgggcga aatttgcgat ttattgcgcc gttatcatca tgtcagccat
    62701 gagttggaaa atggttcgag tgagctctta ttgaaagagc ttaacgaatt gcaacttgaa
    62761 atcgaagcga aggacggttg gaaactggat gcggcagtca agcagacttt gggggaactc
    62821 ggtttgccgg aaaacgaaaa aatcggcaac ctttccggcg gtcagaaaaa acgcgtcgcc
    62881 ttagcgcagg cttgggtaca gaagcccgat gtattgttac tggacgaacc gaccaaccat
    62941 ttggatatcg acgcgattat ttggttggaa aacctgctca aagcgtttga aggcagcttg
    63001 gtcgtgatta cccacgaccg ccgttttttg gacaatatcg ccacgcgcat tgtcgaactc
    63061 gatcgcggta ttttgcgctc ctatcccggc tcgttctcca aatacagcga gaaaaaagcg
    63121 caagagttgg cggttgaagc cgaacacaac cgcctcttcg acaaattcca cgcgcaggaa
    63181 gaggcgtgga tacgcaaagg catcgaagcg cgccgtaccc gcaacgaagg ccgcgtgcgc
    63241 cgtttggaag aactgcgccg ccagcgtgcc gaacgccgca atgtacaagg acaggtcaac
    63301 ttcaaactcg acagcggcaa gaaaagcggc aaaatcatcg ccgaactgga acacgcttcg
    63361 tttgcctatg acgacaaagt tatcatggat aaattttccg ccatcttgca gcgcggcgac
    63421 aaaatcggct taatcggccc aaacggtatc ggcaaaacca ccttccttaa gctgattttg
    63481 ggcgaattgc agccgaccta cggcagaatc cgtatcggca gcaagcagga agtcgcctat
    63541 ttcgaccagt tccgcagcgc cttgaatgaa aacgatactg tgttttacac gctcggacag
    63601 ggcaacgatt acgtcgaagt cggcggcaag aaaaagcatg tgatgagcta tttggaagat
    63661 ttcctgttcc cccctgcccg cgcgcaaagt cctgtttctt cactctccgg cggcgaacgc
    63721 aaccgccttt tgctggcaaa actctttact cgtcccgcca atattttggt tttggacgaa
    63781 ccgaccaacg acttggacat cgacacccaa gaattgcttg aagatctgtt gcgcgattac
    63841 caaggcacgg tcttcctcgt ctcgcacgac cgtatgttcc tcgacaatgt aattacccaa
    63901 agcattgttt tcgaaggaca aggccgtctg aaagaataca tcggcggcta tcaggactat
    63961 atcgacgcaa aatcgcggga aaataaaatt cagacggcat ccgcacccaa agcatccgat
    64021 gtcgaacccg ccaaagaaaa acccaaagcc aaccgtacgg tcaaactttc ctacaaagaa
    64081 cagcgcgaac tcgacgccct gcccgacgaa atcgccgcct tggaaaccga acaggcagaa
    64141 atcaacgcgc agctttccga tcccggaatt ttcaaagatt acgaaaaagc aggagcattg
    64201 caaaaccgcg ccgaagaaat cgaaatgctg cttttggaaa agctggaacg ctgggagctg
    64261 ctggaaacca aacagaacgg caatgccgtc tgaaatcaaa caggaagaaa aaaaatgttc
    64321 aaacgacctg aagaaatcat cgtcctgata cttgccgtcc tctggattgc cggtacgtat
    64381 tttctcgccg ccctgtttgg agcggatgct tataccgtgt taaaaatcac tgcccttacc
    64441 ctgctttggt ctgccgcatc attcctcctt tggcaaaaaa aacctcagcc ggcatatctg
    64501 gccgctgctg ctcggctgcc tgactacctg ttggtggccg tatctgaatc aatcggtcgc
    64561 acccggttct ttaccttggc atgcatcatg gacgttcaaa accatcttag ccctgattcc
    64621 cgcaaccgca ggttatctgt ttaaatggaa acaataccgg cagcgcaaaa caaagccgta
    64681 agcccgtctt ttttcagacg gcatctccgg tcagactgtc aacaattccg ataaaatgtt
    64741 atactcgcgc ccgttgccgc gccgtccgaa accgcctttt gggaaagcag aaatgatcag
    64801 caaacaagaa taccaagccc aagccgccca aggctacaac cgcatcccgc tcgtgcaaga
    64861 actccttgcc gacttggata cgccgctttc cctctatctc aaactcgcca accgccccta
    64921 tacctacctg ctcgaatccg ttgtcggcgg cgaacgtttc ggccgctatt cctttatcgg
    64981 cctgccttgc agccactatc tcaaagccgg cggcaaacac gtcgatgttt atcaaaacgg
    65041 cgaaatcgtc gaacaatacg acggcaatcc gctacccttt atcgaagctt tccacaaccg
    65101 tttcaaaaca cccgaaatcc caagcctgcc gcgctttacc ggcggcttgg tcggctactt
    65161 cggttacgaa accgtctaca atttcgaaca cttcgcccac cgcctgaaaa acaccgccaa
    65221 agccaacccg ctcggcacgc ccgatatttt gctgatgctg tcgcaagagt tggcggtaat
    65281 cgacaatttg agcggcaaaa tccacctcat cgtttatgct gatccctcgc agcccgacag
    65341 ctacgaacgc gcccgcgaac gcctcgaaga catccgcacc cagttgcgcc aaagctgcgc
    65401 catcccactt tccctcggca gcaaacaaac ccaagcagtc agcgagttcg gcgaagaacc
    65461 tttcaaagcc tgcgtcgata aaatcaaaga ctacattttc gcaggcgact gtatgcaggt
    65521 cgtccccagc caacgcatga gcatggaatt taccgacaat ccgcttgccc tctaccgcgc
    65581 cctgcgcacg ctcaaccctt cgccttacct cttttactac gatttcggcg atttccacat
    65641 cgtcggttcc tcgcccgaaa tcctcgtccg ccgcgaacgc gacgacgtca tcgtccgccc
    65701 catcgccggc acgcgcctgc gcggcaaaac ccccgccgaa gaccttgcca acgaacaaga
    65761 tttgttaagc gacgccaaag aaatcgccga acacgttatg cttatcgatt tggggcgcaa
    65821 cgatgtcgga cgcatcagca aaacaggcga agtcaaagtc accgacaaaa tggtgattga
    65881 aaaatactcc catgtgatgc acatcgtttc caacgtcgaa ggctgtctga aagagggcgt
    65941 taccaatatg gacatcctcg ccgccacctt ccctgccggc acactctccg gcgcgccaaa
    66001 agtccgcgcg atggaaatca tcgaagaaat cgaaccggag aaacgcggca tctacggcgg
    66061 cgccgtcggc gtatggggct tcaacaacga tatggatttg gcaatcgcca tccgcaccgc
    66121 cgtgattaaa aacaataccc tcttcatcca aagcggcgcg ggcgtggttg ccgactccga
    66181 cccaacttcc gaatggcagg aaactcaaaa caaagcccgc gcagtggttc gggcggcgca
    66241 gatggtgcag gaagggttgg ataaataaat ttcagacaac cttcgcagat gaaaagaagg
    66301 tcgtctgaaa atcacaaaac aacatcagag atacttacat aaaaaatcac aatgcaattc
    66361 acatcattag aaatttgtgc cggagcaggc ggacaggctt tgggcttaga aagggcaggc
    66421 ttttcccatg ttgcgttaat cgaaatcgaa ccgtcggctt gtcaaaccct acgtttaaac
    66481 cgtcctgatt ggaacgttat tgaaggagat gtccgcttgt ttcagggcga aggatatgac
    66541 ggtattgatt tattggccgg cggcgtgcct tgccctcctt tttccaaagc tggaaaacaa
    66601 ctgggaaaag atgatgaacg cgatttattc cctgaggcta ttcgtctagc taaggaaact
    66661 gaccctaaag ccatcatgtt agaaaatgtg cgggggttac ttgacccaaa atttgaaaat
    66721 tatcgcaacc atattacaga acaatttgcc aagttgggtt accttggaca atggaaacta
    66781 ctttatgctg ccgattacgg agtatcgcaa ctcagaccca gggttttatt tgttgcgttg
    66841 aaaaacgaat acaccaattt tttcaaatgg cctgaaccaa attcggaaca accgaaaacc
    66901 gttggagaac tgctttttga cttaatgtca gaaaataact ggcagggggc gcataactgg
    66961 cgtttgaagg ccgcgcagat tgcaccgacc ttggtcggcg gttcaaaaaa acacggcggt
    67021 gctgacttgg ggcctacacg atccaaacgc gcatgggcgg agttgggtgt ggatggttca
    67081 ggtttgtggg atagtgcgcc gcctgaagac ttttcgggaa tgcctagatt gactgtccga
    67141 atgacggcgc gtatccaagg atttcctgat aactggcaat tttttggtaa aaaaacgcct
    67201 atgtaccgcc aaatcggcaa cgcttttccc ccgccggtag ccgaagcggt gggcaggcag
    67261 attatcaaag ccttaaaaaa ggaaaattga atgaatccgc tttttaccca agaacgcagg
    67321 attttccata agaaattatt ggatggcaat attcttgcaa ccaacaatag aggcgtagtc
    67381 agcaatgcgg atgggagcaa tacccgaagt ttcaatatag ccaaggggat tgccgactta
    67441 ctgcattcgg aaacggtttc ggaaagatta cccggtcaga catcgggaaa tgcttttgaa
    67501 gcaatatgca gtgagtttgt ccaatctgct tttgagaaat tgcagcatat cagacccggc
    67561 gactggaatg ttaagcaggt cggttctcgc aaccgtctgg aaatcgcacg ttatcagcaa
    67621 tacgcacatc tgacagcctt agccaaggct gccgaagaaa accccgaatt ggctgccgcc
    67681 ttgggcagtg attacaccat tactcccgat attatcgtta ccagaaattt gattgcagat
    67741 gcagaaatca accgtaatga atttttagtt gatgaaaata ttgccacata tgccagtttg
    67801 cgtgcgggta atggcaatat gccgctgctc cacgccagta tctcttgtaa atggacgatt
    67861 cgcagcgaca gggctcaaaa tgcccgttct gaaggattga atttggttag aaacagaaaa
    67921 ggcagactgc cgcacattgt tgtcgtaacg gcagaaccaa cgccaagccg catttcatct
    67981 attgccttgg gtacagggga aatagattgc gtttaccatt ttgccttata tgagctggaa
    68041 caaattttac aatcattgaa ttatgaagac gctttggatt tgttttacat catggtcaat
    68101 ggtaaacgat taaaagatat ttccgacctt cccttagatt tggcagttta aacagaatta
    68161 ttccactatg aacacacccc ccatccttcc tcccgccatg ctcggcatcc tcggcggcgg
    68221 acaattaggc agaatgtttg ccgttgccgc taaaaccatg ggctacaaag taaccgttct
    68281 cgatcccgac ccgaatgcgc cggcggcgga atttgccgac cgccatttgt gcgcgccgtt
    68341 tgacgaccgg gccgcgttgg acgaattggc aaaatgcgcg gcggttacga ccgaatttga
    68401 aaacgtcaat gccgacgcga tgcgctctct ggcaaagcat accaacgttt cccccagcgg
    68461 cgactgcgtg tccattgcac aaaaccgcat tcaggaaaaa gcgcggatac gcaaagcagg
    68521 cttgcaaacc gcgccgtatc aggcggtttg caaggccgaa gacattactg aagcaagcgc
    68581 gcaatttttg cccggcatcc tgaaaacggc tacgttgggc tacgacggca aaggtcaaat
    68641 ccgcgtcaaa acgttggacg aactcaaagc cgcgtttgcc gaacacggcg gcgtggattg
    68701 cgttttggaa aaaatggtgg acttgcgcgg cgagatttcc gtgatcgtat gccgtctgaa
    68761 cgatgaaaac gtgcaaacct tcgaccccgc cgaaaacatc cacgaaaacg gcatcttggc
    68821 ttattccatc gtccccgcgc ggctgagtgc cgacgtgcag caacaggcgc ggcagacggc
    68881 gcaacgcttg gcggacgaat tggattatgt cggcgtattg gcggtagaaa tgtttgttgt
    68941 cggcgacaca catgaattgc tcgtcaatga aaccgccccg cgcacgcaca attccggcca
    69001 ccatacgata gatgcctgcg ccgcagacca gttccaacag caggtacgca ttatgtgcaa
    69061 cctgccgccc gccgacacca aattattatc cccttgctgt atggcgaata ttttgggcga
    69121 cgtttggcag gaagatggcg gcgaaccgga ttggctgccg ttgcaaagcc ggccgaatgc
    69181 acacctgcac ctatacggaa aaaaaaccgc acagaaaggt cggaaaatgg gacactttac
    69241 cgttttgacc accgattcgg acaccgcatt tcaagaagca aaaaaactgc atcagtccct
    69301 ataatccgac caacggcaca gatgccgtct gaagccgccg acataccgcc atgagcctgc
    69361 tgacactgtt acgccccgcc accgtgcaag actgcaaaga catcttcaaa gtccacctgc
    69421 actccgtcca atacacctgc atcctcagct acaacgaaca cgccctaaaa gtatgggaag
    69481 gcttgttaaa cacagaaagc tacctgccga ccatttccga tcccgacaaa gcactttggg
    69541 ttgccgaata caaaggcaac atacagggat ttttccaaat cgactgccaa gaagcacaac
    69601 tcgatgcgct ttacgtccac cccctgttcc acaatctcgg actgggcact gccctgctcc
    69661 gtcaggcaga aaccatcgcc cacaaatccg gtttgagctt tctgaaactc tacgcatctc
    69721 tcaactccgt cccgttttac cttctcaacc gctacgaatc gctcggttcg gcagtattgc
    69781 agcttgaccc cagcatcaaa atcaaatgcg aactgatgcg caaacacctc taatcccgcc
    69841 ttataaccaa aactgataat cttcaaatca aacattattt ccacaagcgc agacagccgt
    69901 ataaagttgc cccatctacc aaccgacagg acaagcatat gaaaacctac gcacaggcac
    69961 tctataccgc agccctgctc accgcctgca gccccgcagc cgattcaaac catccgtccg
    70021 gacaaaatgc cccggccaat accgaatccg acggaaaaaa cattaccctg ctcaatgcct
    70081 catacgatgt gacacggtat ttttacaaag aatacaacca cttatttatc aaaacatacc
    70141 aatccgaaca ccccggcaca tccgtcagca tccaacaatc ccacggcggc ttcagcaaac
    70201 aggcattatc cgtagccaac ggccttcaag ccgatgtcgt aaccatgaac caatcttccg
    70261 acatcgacct gctcgaaaaa aaggactggt agaaaaaggc tggcaacaag ccctccccga
    70321 tcacgccgca ccctacacca gcactatggt tttccttgtc cgaaaaaaca accccaaaca
    70381 gatccgcgat tggaacgacc ttgccaaaga cggcgctaac atcgtcatcg ccaagacctc
    70441 gggcaacgga cgctacgcct tcctcggcgc atacggttac ggtctgaaag ccaacaacgg
    70501 caacgagcag gaagcccaaa aactcgtcgc atccatcctc aaaaacacac ccgtttttga
    70561 aaacggcgga cgcgccgccg ccaccacctt cacacaacgc aacatcggcg acgtactcat
    70621 cacttttgaa aacgaagcca actacgtcag caaaaaactg acccaaggac agtttgaaat
    70681 cgtctatccg agctacacca tttccgccga aagccccgtt gccgtcgtca acagcgtcgt
    70741 cgcgaaaaaa ggcacgcaaa aaaccgcccg cgcctatctc gaataccttt ggagcgaacc
    70801 ggcgcaagaa ctcgccgcct cactctacct gcgtccccgc aaccccgaag tattggcacg
    70861 acacaaagcc gacttccccg acttagacac cttccctccc gaagaaaaat tcggcggatg
    70921 ggacaacatc atgaaaacct acttcgccga cggcggcgta ttcgaccgcc tgaccgcaca
    70981 aaaatgacca ttatgccgtc tgaaccgcct ttcagacggc atttcacaca tcaaaacaga
    71041 aaaccgcacc caaaatcatc atcacaagac acaacggcaa accggataca aaacccaaca
    71101 ccgccgccaa aatgccgtct gaagccgtcg gcgcaaccat ccttatctgg ggcgcgcctc
    71161 atcaaagggg cgcataactt tccttaacca tcccttttga cagccaaata aacagtgctt
    71221 ttgcctgtca gaaacaaaac caatattttg cggttcgtat cggaacattt ataaaacatc
    71281 agatttccgt gtttcgcgtt tccggcaatt caagattagc aaaaccatta acctgcttcg
    71341 caaggcaggt ttttaaaact ttatcaggcg ttcgagcata tcggttcgac cgacccgcat
    71401 ttccggcaat caacactgcc gacggttgcc gcatttcttt ttgatataca atcacacttt
    71461 caaaaaacat acgacggcaa caaaatcatc ctccccccgc ttggcaaaca tccggcaaat
    71521 taaaaccccg cccgaaacag ccatcggaca aataacagga aaaaatatga ataccgactt
    71581 ccaaaagatc ctcaaacaac tgggcaaaca ggccaaaaaa gaagcggcag aaaaacagga
    71641 agccgaaaaa aacaagcaaa aacaagaata tgatttggat ttttcacaag cagtcggaca
    71701 agtctcccca ttaaaaaacc ggcagcaata ttatgcgcag tccgacaaaa cccccatcaa
    71761 agcccgtccc aaagacaacc gcaccgacga agaaaactac ttctacatag gcagcacata
    71821 caacgaccca cccgccagct tctgcaaaaa cggacaggga aaaaacgaca tccaacgcct
    71881 gaaaaacgga tactatcccg tcgttaccga tgtcgacctg cacggctaca cccaagaaga
    71941 agcacaaaaa gtcctcaacg aattcatcgc attcacccaa aaacgcggcg tatgcggcga
    72001 aatcatccac ggcagcggat taggttccaa aggctacaaa cccgttctga aaaacatgac
    72061 ccgaaactgg ctgatgcaac accccgacgt actcgcctac gtcgaacccc gggaaggcaa
    72121 cgacggctgc gtccgcatcc tgctcaaacg caaattaagg cagcaagact gacaaatttc
    72181 tccgacagac ttggcagcac cgcacaatat ccgtataatt tgcaaaccat ttctgggggc
    72241 gaccttggtt tcgacggggg ttgcgaagca gatgcgggca taccggggtc tcagattccc
    72301 gtaaaacact gaattcaaat agtcgcaaac gacgaaactt acgctttagc cgcttaaggc
    72361 tagccgttgc agcagtcggt caatgggctg tgtggcgaaa gccaccgcaa cgtcatctta
    72421 cattgactgg tttccagccg ggttacttgg caggaaataa gacttaaggt aactggtttc
    72481 caaaaggcct gttggtcggc atgatggaaa taagattttc aaatagacac aactaagtat
    72541 gtagaacgct ttgtagagga ctttcggacg ggggttcgat tccccccgcc tccaccatgc
    72601 cctattttca gcagtatcct gcaacacaaa aaatccttga acttcaaaag tttcaaggct
    72661 tttttattgc cttgaatatg atggcgtatg cttgagtttt cgcaatttat ggggtaactt
    72721 ttggggtaac ttttcaggaa tcaccctaaa aaatatctta aaaatacaaa agccatcacg
    72781 cattttttaa aaaggttacc caaaacttaa atgaagcctt tatttataag gcttgcagct
    72841 ttccaaacaa gttaccccca aacggataaa aaatgccgaa g
//
LOCUS       DAWTQS010000003        63717 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.003, whole genome shotgun sequence.
ACCESSION   DAWTQS010000003 DAWTQS010000000
VERSION     DAWTQS010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 63717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63717
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.003"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(200..1039)
                     /locus_tag="WNA25_000190"
     CDS             complement(200..1039)
                     /locus_tag="WNA25_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HFA9823053.1"
                     /translation="MKQLRDNKAQGSALFTLVSGIVIVIAVLYFLIKLAGSGSFGDVD
                     ATTEAATQTRIQPVGQLTMGDGIPVGERQGEQIFGKICIQCHAADSNVPNAPKLEHNG
                     DWAPRIAQGFDTLFQHALNGFNAMPAKGGAADLTDQELKRAITYMANKSGGSFPNPDE
                     AAPADNAASGTASAPADSAAPAEAKAEDKGAAAPAVGVDGKKVFEATCQVCHGGSIPG
                     IPGIGKKDDWAPRIKKGKETLHKHALEGFNAMPAKGGNAGLSDDEVKAAVDYMANQSG
                     AKF"
     gene            1324..1399
                     /locus_tag="WNA25_000191"
     tRNA            1324..1399
                     /locus_tag="WNA25_000191"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1357..1359,aa:Arg,seq:ccg)
     gene            complement(1464..2657)
                     /locus_tag="WNA25_000192"
     CDS             complement(1464..2657)
                     /locus_tag="WNA25_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HFA9823054.1"
                     /translation="MYRHIEYYPGDPILSLVETFKNDPRPEKVNLSIGIYFDDEGRMP
                     VLESVSRAETARTAVPAPSPYLPMEGLDVYRSAVQHLLFGKGNPALAQGRIVTVQTLG
                     GSGALKVGADFLHRWFPEARAYVSDPTWDNHRGIFEGAGFEVGTYPYYDPATVGVKFD
                     EMTAFFNTLPENSVLILHPCCHNPTGVDMSERQWDEVLQIIKTRKLIPFMDIAYQGFG
                     GDLDSDAYAVRKAVEMDLPLFVSNSFSKNLSLYGERVGGLSVVCPNKEEADLVFGQLK
                     FTVRRIYSSPPAHGAYIAADVMNSSELYALWQNEVYMMRDRIRAMRQKLYGVLTARIP
                     DRDFTYFIKQRGMFGYTGLSVGQVRRLRDEFAVYLLDSGRMCVAGLNTSNITYVADAL
                     AEVLK"
     gene            complement(2677..3765)
                     /gene="trmA"
                     /locus_tag="WNA25_000193"
     CDS             complement(2677..3765)
                     /gene="trmA"
                     /locus_tag="WNA25_000193"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFA9823055.1"
                     /translation="MNDYTQQLQGKKDYLKTLFAGLDVPEWEVYESPDKHYRMRAEFR
                     IWHEGGEMFYAMFEKGQKASGASLIRCDRFDAASEAVNCLMPELIAVAAQSAELRNHW
                     YAVEFLSTLSGEMLVTMIYHKRLDDEWMKAAQALQQQLDISVIGRSRGQKIVLKQDYV
                     TETLRVGDRDFHYRQIEGSFTQPNAAVCRKMLEWACRAAEGLGGDLLELYCGNGNFTL
                     PLSRYFRQVLATEISKTSVSAAQWNIEANWIGNIKIARLSAEEFTEAYTGKREFTRLK
                     ESGIVLTDYAFSTIFVDPPRAGIDEETLKLVSQFDNIIYISCNPETLRANLDTLTETH
                     TVGRAALFDQFPFTHHIESGVLLKKKIL"
     gene            3912..5012
                     /gene="aroC"
                     /locus_tag="WNA25_000194"
     CDS             3912..5012
                     /gene="aroC"
                     /locus_tag="WNA25_000194"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFA9823056.1"
                     /translation="MAGNTFGQIFTVTTFGESHGAGLGCIIDGCPPGLELSEADIQFD
                     LDRRKPGTSRHVTQRREADQVEILSGVFEGKTTGTPIALLIRNTDQRSKDYGNIATAF
                     RPGHADYTYWHKYGTRDYRGGGRSSARETAARVAAGAVAKKWLKEKFGTEITAYVTQV
                     GEKKIRFEGSEHISQNPFFAANQSQIAELEHYMDGVRKSLDSVGAKLHIEAANVPVGL
                     GEPVFDRLDAEIAYAMMGINAVKGVEIGAGFDSVTQRGSEHGDELTPQGFLSNHSGGI
                     LGGISTGQDICVNIAIKPTSSIATPRRSIDIHGNPVELATRGRHDPCVGLRAAPIAEA
                     MLALVLIDHALRHRAQNADVAADTPDISRSDK"
     gene            5103..5528
                     /locus_tag="WNA25_000195"
     CDS             5103..5528
                     /locus_tag="WNA25_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant transport activator ProQ"
                     /protein_id="HFA9823057.1"
                     /translation="MTQETALGAALKSAVQTMSKKKQTEMIADHIYGKYDVFKRFKPL
                     ALGIDQDLIAALPQYDSALIARVLANHCRRPRYLKALARGGKRFDLNNRFKGEVTPEE
                     QAIAQNHPFVQQALQQQSAQAAAETPSVEAEAAESSAAE"
     gene            complement(5602..7587)
                     /gene="parE"
                     /locus_tag="WNA25_000196"
     CDS             complement(5602..7587)
                     /gene="parE"
                     /locus_tag="WNA25_000196"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFA9823058.1"
                     /translation="MAKNNQYSESSITVLKGLEPVKERPGMYTRTDSPTHICQEVIDN
                     AADEALGGFATEIDVQIHEDGSLSVRDNGRGIPVGLHPEEGVPVVELVFTRLHAGGKF
                     NKKDGGSAYAFSGGLHGVGVSVTNALSTRLEVSVKRDGKVHRIVFAGGDVVEPLAQVG
                     KCAVKDSGTEVRVWPDGKYFESPNYSIPELERLLRAKAVLLPGVRVSLTRPVKGEDEA
                     HTQTWHYPDGLKSYLTDLIADAQEAVPLFSCENYISDGHNGDFSIGEGAAFALTWLEE
                     GSCANESYVNLIPTSLGGTHEAGLKQAVFNAVNNFINLHNLLPRGVKVQSDDVFGKTA
                     FVLSARVLDPQFQGQTKDKLTNRDALKLVAAVSGDPLELWLNQNVDFGKKIAELAIRQ
                     AQARIRSVKKIEKKKGSGVAILPGKLTDCESEDIRENELFLVEGDSAGGSAKLARDKA
                     TQAILPLRGKVLNSFEVHPDQLFGNAEIHDISVAIGVDPHAINDHPDLSGLRYGKIAI
                     LSDADVDGSHIQVLLLTLFYRHFPKLVADGHIYVAQPPLFRVDVNAQGKSKPARKFYA
                     LDQNELDGILERLQKEGVKETAYSISRFKGLGEMNPDQLKDTTMHPDTRRLLQVQIPE
                     GADDETRDIFVKLMGKGEAAARRAWMEREGDTAQLDI"
     gene            complement(7654..8178)
                     /locus_tag="WNA25_000197"
     CDS             complement(7654..8178)
                     /locus_tag="WNA25_000197"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFA9823059.1"
                     /translation="MLDREGYRPNVGIILINERNEVFWGKRVREHSWQFPQGGIKPGE
                     SPETAMYRELYEEVGLLPQHVKIVGRTRDWLRYDVPNNWVRREWRGSYRGQKQIWYLL
                     RLTGRDCDVNLRATRHPEFDGWRWHQYWAPVDEVIDFKRDVYLGALKELSSRFLRGME
                     SYEDFAARQPSGNR"
     gene            8344..9639
                     /gene="serS"
                     /locus_tag="WNA25_000198"
     CDS             8344..9639
                     /gene="serS"
                     /locus_tag="WNA25_000198"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFA9823060.1"
                     /translation="MLDIQLLRSNTAAVAERLARRGYDFDTARFDALEERRKSVQVKT
                     EELQASRNSISKQIGALKGQGKHEEAQAAMDQVAQIKTDLEQAAADLDAVQKELDAWL
                     LSIPNLPHESVPPGKDETENVEVRKVGTPREFDFEIKDHVDLGEPLGLDFEGGAKLSG
                     ARFTVMRGQIARLHRALAQFMLDTHTLQHGYTEHYTPYIVDDTTLQGTGQLPKFAEDL
                     FHVTRGGDETKTTQYLIPTAEVTLTNTVAGSIIPSEQLPLKLTAHSPCFRSEAGSYGK
                     DTRGLIRQHQFDKVEMVQIVHPEKSYGALEEMVGHAENILKALELPYRVITLCTGDMG
                     FSAAKTYDLEVWVPAQNTYREISSCSNCEDFQARRMKARFKDENGKNRLVHTLNGSGL
                     AVGRTLVAVLENHQNADGSINIPAALQPYMGGVTKLEVK"
     gene            complement(9712..10710)
                     /locus_tag="WNA25_000199"
     CDS             complement(9712..10710)
                     /locus_tag="WNA25_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFA9823061.1"
                     /translation="MKIAIYGTKSYDREHFTRANRHFGLELEFFDFMLDAKTAKMAEG
                     AEAACIFVNDDASRPVLEKLAQIGVKTVALRCAGFNNVDLKAAEELGLKVVRVPAYSP
                     ESVAEHTVGLMLTLNRRIHKAYQRTRDANFSLEGLTGFNMYGKTAGVIGTGKIGIATM
                     RILKGFGMNLLAYDPFCNPEVEKLGGRYVDLDELYARSDIITLHCPATPENHYMLNEA
                     AFDKMKDGVMIINTSRGGLIDSAAAIEALKCRKIGALGMDVYENERELFFEDKSNDVI
                     TDDVFRRLSSCHNVLFTGHQAFLTEEALGNISEVTLSNIREVGQTGDCGNAVRADG"
     gene            complement(10835..11911)
                     /gene="prfA"
                     /locus_tag="WNA25_000200"
     CDS             complement(10835..11911)
                     /gene="prfA"
                     /locus_tag="WNA25_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFA9823062.1"
                     /translation="MKPSILEKLQQLGDRLEEVTHLLGQPEATSDMDNYRKLTREHAE
                     LTPVVEVFQNYRLAQSDLADAEEMLSDPEMKDFAAEEIEAAKAKIDELDTELQKLLLP
                     KDADDDKNIFIEIRAGTGGGEAALFAGDLLRMYSRYAERNRWQVEIVSANESELGGYK
                     EVIARIVGLGAYSRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPEADELEDIELN
                     PADLRTDTFRASGAGGQHINKTDSAVRITHLPTGMVVECQDGRSQHANKAQAMKVLAA
                     RLNDAQKREVQAKEAAERKSLIGSGDRSERIRTYNYPQGRVTDHRINLTLHKLDFVMD
                     GDLAEITDALIAEHQAELLAAMGD"
     gene            complement(12001..12771)
                     /locus_tag="WNA25_000201"
     CDS             complement(12001..12771)
                     /locus_tag="WNA25_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFA9823063.1"
                     /translation="MRLTDTHCHLADSVLRGNLAQVLAEARQAGVWRFIVPATCPQDW
                     QDVAELSEMPSEHGQIRIALGIHPWFSDGIAEQDFSGLEAVLVRYPRAWVGEIGLDFY
                     DKTQTPPQRERQIQVFSRQLAIAQTLRRRVIIHNLKATADIAAAVKQTGFTQGGIVHA
                     FSGSAEEARVLTKLGFKIGIGSLLLNPNARKVRDTLKALNDGDFVLETDSPFMLENTV
                     NTPANILRIAEIAAEIRGTGAAEIAAITERNADSLLRP"
     gene            complement(12774..13766)
                     /locus_tag="WNA25_000202"
     CDS             complement(12774..13766)
                     /locus_tag="WNA25_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFA9823064.1"
                     /translation="MKQKIFVLYTGGTIGMTQSSAGLRPDTALVGQALAPFSDGMDFD
                     WHVCEPLIDSSAVQLQNWCAWLDIIAAALPQYDGVLVLHGTDTLAYTANMFALALQGL
                     DKPIVLTGSQWPYNAEGSDAPRNLVTAVAAFPLGLKQTLIAFDGKLYPAVGSSKISTE
                     TAAGFGNAHFGALAEWDETRGWHNVRIPNQDAADVSDGLEIRYPDPQAKIAVRTLIPG
                     FAVQELADGLGQLPAQALILQSYGHGNTSAGEGFIRAVQDFTQQGKLLLNISQVPQGC
                     AAAVYAQGDALRRAGVVNGGKCNLETATALMTLAVSEGWGADGVKHELQRLGLV"
     gene            complement(13833..14516)
                     /locus_tag="WNA25_000203"
     CDS             complement(13833..14516)
                     /locus_tag="WNA25_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFA9823065.1"
                     /translation="MFALLEAFFVEYGYAAVFFVLVICGFGVPIPEDLTLVTGGVISG
                     MGYTNPHIMFAVGMLGVLAGDGVMFAAGRIWGQKILKFKPIARIMTPKRYAQVQEKFD
                     KYGNWVLFVARFLPGLRTAVFVTAGISRKVSYLRFLIMDGLAALISVPVWIYLGEYGA
                     HNIDWLMAKMHSLQSGIFIALGVLAAALAWFWWRKRRHYQLYRAQLSEKRAKRKAEKA
                     AKKAAQKQQ"
     gene            complement(14705..16039)
                     /gene="glmM"
                     /locus_tag="WNA25_000204"
     CDS             complement(14705..16039)
                     /gene="glmM"
                     /locus_tag="WNA25_000204"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFA9823066.1"
                     /translation="MAKKYFGTDGVRGEVGQFPITPDFVLKLGYAAGQVLVQHDTDQK
                     PTVLIGKDTRISGYMLEAALVAGFTAAGVNVVQTGPLPTPGVAYLTRALRLSAGVMIS
                     ASHNTYSDNGIKFFAEGGVKLSDEVELEIEAKIDEEMKTQPSARLGRARRISGADDRY
                     IEFCKSTFPGHSDLRGLKLVIDTANGAGYGVAPKVFHELGAQVVSIGNEPNGYNINEK
                     CGATHTKTLQAAVLQNEADYGIALDGDGDRLMMVDKNRQVYDGDSLIYVIAKARAREG
                     INIGGVVGTVMTNMAMEIALKEQGVDFCRAKVGDRYVLEQLNQRGWLIGGEASGHILC
                     MDKHNTGDGIISALQVLAALQILNQDLATVCADWQPYPQTMINVRIQKGQKWQEASKD
                     VLAEVEKELEGKGRVVLRASGTEPVVRVMVEARQADWARDGAERIAAAIGGI"
     gene            complement(16169..17020)
                     /gene="folP"
                     /locus_tag="WNA25_000205"
     CDS             complement(16169..17020)
                     /gene="folP"
                     /locus_tag="WNA25_000205"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFA9823067.1"
                     /translation="MARHVWRAGRFEIGLDKPKIMGIVNLTPDSFSDGGAYSQNAQTA
                     LAHAERLLKEGADILDIGGESTRSGADFVPPEEEWARVEPVLAEAAGWGVPVSLDTRR
                     TVVMEKALALGGIDIINDVAALTDEGAVELLARQADTGICLMHMRGLPETMQDNPKYQ
                     DVVGEVARYLKTRSETCVAAGIAPQRITLDPGFGFGKNLQHNIALMRHLPELMAETGL
                     PLLIGVSRKSMIGKLTGEADAAARVHGSVAAALASVARGAQIVRVHDVKATADALKVW
                     EALGVNR"
     gene            complement(17160..18053)
                     /locus_tag="WNA25_000206"
     CDS             complement(17160..18053)
                     /locus_tag="WNA25_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFA9823068.1"
                     /translation="MIIVMSRRAAEADIAGVVAFIRSRGLREHISHGDERTVIGAIGD
                     DRVLSVREVQTLPEVEKAVRILDTWKTVSRENRAEDSRVAAKGVAFGGGETVRIAAEP
                     SVWSNADAVFLDPFFTSANLYDTLSADEGRGRCRRLAEQAASAHGAGKPVLVRVRNVR
                     HVEAALNAGADILYLGGGLMSDLAVLNEAGSLNIPLVLCKDKHHSAEDWLNAAEYVVS
                     RGNRHLILGESGVLGHTKGHPYRLDVESIVKVRQISHLPVIANITGLWSRDMPQEILY
                     GLAKAAGACGIVGTCFEKAGG"
     gene            18144..20246
                     /locus_tag="WNA25_000207"
     CDS             18144..20246
                     /locus_tag="WNA25_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFA9823069.1"
                     /translation="MDLLSVFHKYRLKYAVAVLTMLLLAAVGLHASVYRTFTPENIRS
                     RLQQSIAHTHRKISFDADIRRRLLPRPTVILKNLTITEPDGGRVAVSVKETKIGLSWK
                     NLWSDRIQVEKWVVSGADLALTRDRNGAWNIQDLFDGAKHSASVNRIIVENSTVRLNF
                     LQQQLILKEISLNLQSPDSSGQQFESSGILVWRKLSVPWKSRGLFLSDGIGTPEISPF
                     HFEASTSLDGHGITISTTGSPSVRFNAGGADAAGLGLRADTSFRNLHLTAQIPALALK
                     NNSIKTGTVNGTFTAGGEYARWDGSFKLDKANLHSGIANIGNAEISGSFKTPRLQTNF
                     SLGSPLVWSRDNGLDAPRLHISTLQDTVDRLPQPRFISRLDGSLSIPNLQNWNAELNG
                     TFDRQPVAAKFKYTREGAPHLEAAAALQKLNLAPYLDEFRQQNGKIFPDILGRLSGNV
                     EAHLKIGSIQLPGLQLDDMETYLHADKDHIALSRFKSGLYGGHTEGGISIANTRPATY
                     RLQQNASNIQIQPLLQDLFGFHSFSGNGDAVIDLTASGENRKQLIRSLQGSLSLNISN
                     GAWHGIDMDSILKNGLSGKISGSTPFYRFTLNSEISDGISRHIDTELFSDSLYVTSNG
                     YTNLDTQELSEDVLIRNAVHPKNKPIPLKITGTVDKPSITVDYGRLTGGINSRKEKQK
                     ILEDTLLEQWQWLKPKEP"
     gene            complement(20310..21788)
                     /gene="ubiD"
                     /locus_tag="WNA25_000208"
     CDS             complement(20310..21788)
                     /gene="ubiD"
                     /locus_tag="WNA25_000208"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFA9823070.1"
                     /translation="MKYKDLRDFIAMLEQEGKLKRVAHPVSPHLEMTEIADRVLRAEG
                     PALLFENPIKPDGTRYDYPVLANLFGTPERVAMGMGADSVSKLREIGQTLAYLKEPEP
                     PKGIKDAFSKLPLLKDIWSMAPNVVKNAPCQEIVWEGEDVDLYQLPIQHCWPEDVAPL
                     VTWGLTVTRGPHKKRQNLGIYRQQLIGKNKLVMRWLSHRGGALDYQEFRKLNPDTPYP
                     VAVVLGCDPSTILGAVTPVPDTLSEYQFAGLLRGSRTELVKCIGSDLQVPARAEIVLE
                     GVIHPNETALEGPYGDHTGYYNEQGHFPVFTVERITMRENPIYHSTYTGKPPDEPAVL
                     GVALNEVFVPLLQKQFSEITDFYLPPEGCSYRMAVVSMKKQYAGHAKRVMTGCWSFLR
                     QFMYTKFIIVVDDDVNVRDWKEVIWAVTTRMDPVRDTVLVENTPIDYLDFASPVSGLG
                     GKMGLDATSKWPGETDREWGRVIKKDPAVTVKIDGIWGKLGL"
     gene            complement(21808..22323)
                     /locus_tag="WNA25_000209"
     CDS             complement(21808..22323)
                     /locus_tag="WNA25_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2199 domain-containing protein"
                     /protein_id="HFA9823071.1"
                     /translation="MHTCTSCGEKHEDMPATGFTAPYPYNQLSEEERIAYQAECDSDF
                     CIIRYSGQTDRFIRAALPIPIIGHQETLEYGVWVSVSEKSFNDYQSRFYDNPENAVYF
                     GMICNRLPPYETDTFGLHCNVVTQSDNQRPLLQLHQSGGHPPVRDFYHGMGYAEAQAR
                     IGAVSESDCSG"
     gene            complement(22401..22698)
                     /locus_tag="WNA25_000210"
                     /pseudo
     CDS             complement(22401..22698)
                     /locus_tag="WNA25_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249704.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            22695..22802
                     /locus_tag="WNA25_000211"
                     /pseudo
     CDS             22695..22802
                     /locus_tag="WNA25_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(22960..23772)
                     /locus_tag="WNA25_000212"
                     /pseudo
     CDS             complement(22960..23772)
                     /locus_tag="WNA25_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
     gene            23972..24190
                     /locus_tag="WNA25_000213"
                     /pseudo
     CDS             23972..24190
                     /locus_tag="WNA25_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702943.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24502..25167
                     /gene="fabG"
                     /locus_tag="WNA25_000214"
     CDS             24502..25167
                     /gene="fabG"
                     /locus_tag="WNA25_000214"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFA9823072.1"
                     /translation="MADDGFDIAVHCRSRRDEAEAVAEEIRALGRNARVLQFDVSDRE
                     ACREILTADIEANGAYYGVVLNAGLTRDNAFPAFSDDDWDVVPRTNLDGFYNVLHPLV
                     MPMIRRRKAGRIVCMASVSGLTGNRGQVNYSASKAGIIGAAKALAVELAKRKITVNCV
                     APGLIDTEIVDENVPVGEILKAVPAARMGLPEEVAHAVHFLMDEKAAYITRQVIAVNG
                     GLC"
     gene            25161..25979
                     /locus_tag="WNA25_000215"
                     /pseudo
     CDS             25161..25979
                     /locus_tag="WNA25_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714702.1"
                     /note="FabB; beta-ketoacyl-ACP synthase I, KASI; catalyzes
                     a condensation reaction in fatty acid biosynthesis:
                     addition of an acyl acceptor of two carbons from
                     malonyl-ACP; required for the elongation of short-chain
                     unsaturated acyl-ACP; frameshifted; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
     gene            25956..26506
                     /locus_tag="WNA25_000216"
                     /pseudo
     CDS             25956..26506
                     /locus_tag="WNA25_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706948.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
     gene            26560..26635
                     /locus_tag="WNA25_000217"
     tRNA            26560..26635
                     /locus_tag="WNA25_000217"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26593..26595,aa:Ile,seq:cat)
     gene            26710..27468
                     /locus_tag="WNA25_000218"
     CDS             26710..27468
                     /locus_tag="WNA25_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFA9823073.1"
                     /translation="MKKVSVLIVAKNEANHIRECIESCRFDKEVIVIDDHSADNTAEI
                     AEGLGAKVFRRHLNGDFGAQKTFAIEQAGGEWVFLIDADERCTPELSDEISKIVRTGD
                     YAAYFVERRNLFPNHPATHGAMRPDSVCRLMPKKGGSVQGKVHETVQTPYPERRLKHF
                     MYHYTYDNWEQYFNKFNKYTSISAEKYREQGKPVSFVRDIILRPIWGFFKIYILNKGF
                     LDGKMGWIMSVNHSYYTMIKYVKLYYLYKSGGKF"
     gene            27469..28533
                     /locus_tag="WNA25_000219"
     CDS             27469..28533
                     /locus_tag="WNA25_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFA9823074.1"
                     /translation="MEKEFRILNIVSAKIWGGGEQYVYDVSKALGLRGCTMFTAVNKN
                     DELMRRRFSEVSSVFTTRLHTFNGLFSLYALTRFIRENHISHLMIHTGKIAALSVLLK
                     KLTGVRLIFVKHNVVANKTDFYHRLIQKNTDRFICVSRLVYDVQTADNPFKEKYRIVH
                     NGIDTGRFPPSQEKPDSRFFTVAYAGRISPEKGLENLIEACVILHRKYPQIRLKLAGH
                     GHPDYMCRLKRGVSASGAEPFVSFEGFTEKLASFYRQSDVVVLPSLVPEAFGLSLCEA
                     MYCRTAVISNTLGAQKEIIEHHQSGILLDRLTPESLADEIERLVLNPEAKNALATAGH
                     QCVAARFTINHTADKLLDAI"
     gene            28767..30104
                     /locus_tag="WNA25_000220"
     CDS             28767..30104
                     /locus_tag="WNA25_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HFA9823075.1"
                     /translation="MSNHTSWSSKIGFVLAAAGSAIGLGAIWKFPYTAGTNGGAVFFL
                     LFLIFTVLVALPVQLAEFYIGRTGGKNAVDSFRVLRPGTQWLWVGRMGVAACFILLSF
                     YSVVGGWVLNYVVHSFTGAIHAGADFEALFGTTISNPAGSLSYQALFMLITVWVVKGG
                     ISDGIEKANRYLMPGLFILFIALAVRSLTLPDAMEGVSFLLKPNWSYFKADTMITALG
                     QAFFALSIGVSAMITYASYLGKDQDMFRSGHTIMWMNLLVSLLAGLVIFPAVFAFGFE
                     PNQGPGLIFIVLPAVFMKMPFGTVLFAVFMLLVVFATLTSAFSMLETVIASTIRQDER
                     KRKKHTWLIGTAIFIVGIPSALSFGAWGEFKVFGKTIFDLWDYVISAVIMPIGALSVS
                     IFTAWIQDKQSVLKDAGAGSTVPRAVLLLWLNTLRYLAPIAIIIVFVNSLGIL"
     gene            30171..30275
                     /locus_tag="WNA25_000221"
                     /pseudo
     CDS             30171..30275
                     /locus_tag="WNA25_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(30279..30878)
                     /locus_tag="WNA25_000222"
     CDS             complement(30279..30878)
                     /locus_tag="WNA25_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFA9823076.1"
                     /translation="MWKLYADYFAGRPDAETPPENSVKETLKRVGFGKVSFDDREIRF
                     AEKGMGLSLNGIAQGYITDKVVALLKANGVPAALVDMGEIRGFDTNGRRMWNVGIRNP
                     DDEEGVLANITMKDKAFATSGGYGTVMDKAGRLTHLFDPRTGVSTPRYKSMSVMADDA
                     AVADALSTAFSVMDLPLIRSVAESRRLKVRLAMPDNIVD"
     gene            complement(30911..31291)
                     /locus_tag="WNA25_000223"
     CDS             complement(30911..31291)
                     /locus_tag="WNA25_000223"
                     /inference="COORDINATES: protein motif:HMM:NF014477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFA9823077.1"
                     /translation="MGRHFGRRRFLTAAAVAVAGAAVSFLPNPFAAGGEKRNMDKKRD
                     ENVFFWKGVALGSGAELRLFGVDDRQAADLVNKVLAEVARLEKMFSLYREDSLISRLN
                     RDGYLTSPPADFLELLSLAAIFTR"
     gene            complement(31300..32094)
                     /locus_tag="WNA25_000224"
     CDS             complement(31300..32094)
                     /locus_tag="WNA25_000224"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFA9823078.1"
                     /translation="MKAYLDLMRHVLDNGTDKSDRTGTGTRSVFGYQMRFDLGKGFPL
                     LTTKKLHLRSIIHELLWFLKGDTNIKYLKDNNVSIWDEWADENGDLGPVYGYQWRSWP
                     APDGRHIDQIANVVEQIKKNPDSRRLIVSAWNPALVDEMALPPCHALFQFYVADGKLS
                     CQLYQRSADIFLGVPFNIASYALLTMMMAQVCGLEAGEFVHTFGDAHLYRNHFEQAAL
                     QLEREPRALPVMKINPEVKDLFAFKFEDFELEGYDPHPHIKAVVSV"
     gene            32518..33852
                     /gene="gdhA"
                     /locus_tag="WNA25_000225"
     CDS             32518..33852
                     /gene="gdhA"
                     /locus_tag="WNA25_000225"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFA9823079.1"
                     /translation="MTDLNTLFANLKQRNPNQEPFHQAVEEVFMSLDPFLAKNPKYTQ
                     QSLLERIVEPERVVMFRVTWQDDKGQVQVNRGYRVQMSSAIGPYKGGLRFHPTVDLGV
                     LKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMTELYRHIGADTD
                     VPAGDIGVGGREIGYLFGQYKKIRNEFTSVLTGKGLEWGGSLIRPEATGYGCVYFAQA
                     MLQTRNDSFEGKRVLISGSGNVAQYAAEKAIQLGAKVLTVSDSDGFVLFPDIGMTEAQ
                     LAALIELKEVRRERVATYAKEQGLQYFENQKPWGVAAEIALPCATQNELDEEAAKTLL
                     ANGCHVVAEGANMPSTLGAVEQFIKAGILYAPGKASNAGGVATSGLEMSQNAIRLSWA
                     REEVDSRLFGIMQSIHESCLKYGKVGDKVNYVNGANIAGFVKVADAMLAQGF"
     gene            33947..34053
                     /locus_tag="WNA25_000226"
                     /pseudo
     CDS             33947..34053
                     /locus_tag="WNA25_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(34091..34870)
                     /locus_tag="WNA25_000227"
     CDS             complement(34091..34870)
                     /locus_tag="WNA25_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HFA9823080.1"
                     /translation="MKLVRPQKISDQVLSVLEGRIVEGVYAEGGKIPPERVLAEEFGV
                     SRPSVRSALNILVARQILEARQGDGYYVSVKPQQDFLQSWQELLGKHSNWEQDVFDFS
                     CHIEGCMAALAAERRTDADLKRIRFWLEKFEEACGSGNLEHQGEADVSFHQTIADAAH
                     NLLFSHLSGGLLKMLYRQTRSSLIYLNQEEDPRPKLMAQHRVLYEAISNRRPGEASEA
                     AKAHLNYVASSILKDREYQSRNRHADTLAQNDLKRVQDWEV"
     gene            35148..35447
                     /locus_tag="WNA25_000228"
     CDS             35148..35447
                     /locus_tag="WNA25_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFA9823081.1"
                     /translation="MQRCFNWFDHYCRRLEKYFRRCSIMETWVQNYTAIGGSLYLTAA
                     AALLPIVFFFAALTVLKLKGYQAGLYTLLIALAVAVFGFGMPTGMAVSSLSPQPD"
     gene            35432..35621
                     /locus_tag="WNA25_000229"
                     /pseudo
     CDS             35432..35621
                     /locus_tag="WNA25_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003757319.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(35733..37136)
                     /gene="mtrE"
                     /locus_tag="WNA25_000230"
     CDS             complement(35733..37136)
                     /gene="mtrE"
                     /locus_tag="WNA25_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MtrE"
                     /protein_id="HFA9823082.1"
                     /translation="MDTILKTTLTSVAAAFALSACTMIPQYEQPKVEVAETFKNDTAD
                     SGIRAVDLGWHDYFADPRLQKLIDIALERNTSLRTAVLNSEIYRKQYMIERNNLLPTL
                     AANANGSRQGSLSGGNVSSSYNVGLGAASYELDLFGRVRSSSEAALQGYFASTANRDA
                     AHLSLIATVAKAYFNERYAEEAMSLAQRVLKTREETYKLSELRYKAGVISAVALRQQE
                     ALIESAKADYAHAARSREQARNALATLINQPIPDDLPAGLPLDKQFFVEKLPAGLSSE
                     VLLDRPDIRAAEHALKQANANIGAARAAFFPSIRLTGSVGTGSAELGGLFKSGTGVWS
                     FAPSIALPIFTWGTNKANLDAAKLRQQAQIVAYESAVQSAFQDVANALAAREQLDKAY
                     AALSKQSRASKEALRLVGLRYKHGVSGALDLLDAERSSYSAEGAALSAQLTRAENLAD
                     LYKALGGGLKRDTQTGK"
     gene            complement(37192..40395)
                     /gene="mtrD"
                     /locus_tag="WNA25_000231"
     CDS             complement(37192..40395)
                     /gene="mtrD"
                     /locus_tag="WNA25_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MtrD"
                     /protein_id="HFA9823083.1"
                     /translation="MAKFFIDRPIFAWVISIFIIAAGIFGIKSLPVSQYPSVAAPNIT
                     LRATYPGASAQVMEDSVLSVIERNMNGVEGLDYMSTSADSSGSGSVSLTFTPETDEDL
                     AQVEVQNKLSEVLSMLPATVQQYGVTVSKARSNFLMIVMLSSDVQSTEEMNDYAQRNI
                     VPELQRIEGVGQVRLFGAQRAMRIWVDPKKLQNYNLSFADIGSALSAQNIQISAGSIG
                     SLPAVQGQTVTATVTAQGQLSTAEEFGNVILRANTDGSNVYLKDVAKVGLGMEDYSSS
                     TRLNGVNTTGMAVMLSNSGNAMATAKAVKERMATLEKYFPQGMSWKTPYDTSKFVEIS
                     IEKVIHTLLEAMVLVFVVMYLFLQNIRYTLIPTIVVPISLLGGFAFISYMGMSINVLT
                     MFAMVLVIGIVVDDAIVVVENVERIMAGEGLPPKAATKKAMGQISGAVIGITAVLMSV
                     FVPLAMFSGATGNIYKQFALTMAASIAFSAFLALTLTPALCATMLKTIPKGHHEEKKG
                     FFGWFNKKFDNWTHGYEGWVAKVLRKTLRMMVVYVGLAVVGVFLFMRLPTSFLPTEDQ
                     GFVMVSVQLPAGATKERTDATLAQVTQLAKSIPEIENIITVSGFSFSGSGQNMAMGFA
                     MLKDWNERKTPGSDAESVAGKLTGMMMGTLKDGFGIAIVPPPIMELGNGSGLSINLQD
                     RNNTGHAALLAKRNELIQKMRTSGLFNPSTVRAGGLEDAPQLKIDINRAAAAAQGVSF
                     ADIRTALASALSSSYVSDFPNQGRLQRVMVQADGDARMQPADILNLTVPNSSGIAVPL
                     SSIATVSWQMGTEQSVRFNGYPAMELSGSPATGVSTGQAMEAVQKMVDELGSGYSLEW
                     GGQSREEAKGGSQTIALYALAAVAVFLVLAALYESWSIPLAVLLVMPLGLAGAAAGVT
                     GRNLFEGLLGSVPSFANDIYFQVGFVTVMGLSAKNAILIIEFAKDLQAQGKSAVEAAL
                     EAARLRFRPIIMTSFAFILGVVPLYIAGGASSASQRAIGTTVFWGMLIGTLLSVFLVP
                     LFYVVVRKFFKETAHEHEMAVKHAAEAGITGSDDSQH"
     gene            complement(40407..41645)
                     /gene="mtrC"
                     /locus_tag="WNA25_000232"
     CDS             complement(40407..41645)
                     /gene="mtrC"
                     /locus_tag="WNA25_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit MtrC"
                     /protein_id="HFA9823084.1"
                     /translation="MAFYASKAMRAAALAASVALALSSCGKADEAAQGGQPAGREAPA
                     PVVGVVTVHPQTVALTVELPGRLESLRTADVRAQVGGIIQKRLFQEGSYVRAGQPLYQ
                     IDSSTYEASLESARAQLATAQATLAKADADLARYKPLVAADAISKQEYDAAVTAKRSA
                     EAGVKAAQAAIKSAGISLNRSRITAPISGFIGQSKVSEGTLLNAGDTTVLATIRQTNP
                     MYVNVTQSASEVMKLRRQIAEGKLLAADGVIAVGIKFDDGTVYPEKGRLLFADPTVDE
                     STGQITLRAAVPNDQNILMPGLYVRVLMDQVAVDNAFIVPQQAVTRGTKDTVMIVNAQ
                     GGMEPREVTIAQQQGANWIVTSGLKDGDKVVVEGISIAGMTGAKKVTPKEWMPSENQA
                     AAPQSGVQTASEAKPASEAE"
     gene            41893..42525
                     /gene="mtrR"
                     /locus_tag="WNA25_000233"
     CDS             41893..42525
                     /gene="mtrR"
                     /locus_tag="WNA25_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux system transcriptional
                     repressor MtrR"
                     /protein_id="HFA9823085.1"
                     /translation="MRKTKTEALKTKEHLMLAALETFYRKGIARTSLNEIAQAAGVTR
                     GALYWHFKNKEDLFDALFQRICDDIENCIAQDAANAEGGSWTVFRHTLLHFFERLQSN
                     DIHYKFHNILFLKCEHTEQNAAVIAIARKHQAIWREKITAVLTEAVENQDLADDLDKE
                     TAVIFIKSTLDGLIWRWFSSGENFDLGKTAPRIIGIIMDNLENHPCLRRK"
     gene            complement(42618..42830)
                     /locus_tag="WNA25_000234"
     CDS             complement(42618..42830)
                     /locus_tag="WNA25_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823086.1"
                     /translation="MKNEIQKIMDKYDPWHEDDFESYEDIAKDVSLMTDKTFIEHYLL
                     EVYSEENGHFDQDNVHAMIGEIKNAI"
     gene            43162..44730
                     /gene="mtrF"
                     /locus_tag="WNA25_000235"
     CDS             43162..44730
                     /gene="mtrF"
                     /locus_tag="WNA25_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041421321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family antimetabolite efflux transporter
                     MtrF"
                     /protein_id="HFA9823087.1"
                     /translation="MSQTDARRSGRFLRTVEWLGNMLPHPVTLFIIFIVLLLIASAVG
                     AYFGLSVPDPRPVGAKGRADDGLIHVVSLLDADGLIKILTHTVKNFTGFAPLGTVLVS
                     LLGVGIAEKSGLISALMRLLLTKSPRKLTTFMVVFTGILSNTASELGYVVLIPLSAVI
                     FHSLGRHPLAGLAAAFAGVSGGYSANLFLGTLDPLLAGITQQAAQIIHPDYVVGPEAN
                     WFFMVTSTFVIALIGYFVTEKIVEPQLGPYQSDLSQEEKDIRHSNEITPLEYKGLIWA
                     GVVFVALSALLAWSIVPADGILRHPETGLVAGSPFLKSIVVFIFLLFALPGIVYGRIT
                     RSLRGEREVVNAMAESMSTLGLYLVIIFFAAQFVAFFNWTNIGQYIAVKGAVFLKEVG
                     LAGSVLFIGFILICAFINLMIGSASAQWAVTAPIFVPMLMLAGYAPEVIQAAYRIGDS
                     VTNIITPMMSYFGLIMATVMKYKKDAGVGTLISMMLPYSAFFLIAWIALFCIWVFVLG
                     LPVGPGAPTLYPAP"
     gene            complement(44800..48009)
                     /gene="recC"
                     /locus_tag="WNA25_000236"
     CDS             complement(44800..48009)
                     /gene="recC"
                     /locus_tag="WNA25_000236"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFA9823088.1"
                     /translation="MFYLYQSNRLESLAALFARIQKVKPLKCALQPEQIVVQSQGMRR
                     YLNTCLARDLGVAANLAFSLPAGLTWKLMKKLIPGIPELSPFAPEVMRWRLLDLFRSA
                     EFQNGAEFEDVRNVLQDYLGSGESADYQLAGQLADIFDQYLVYRPQWIDAWQQGRILG
                     LGDDEIWQSKLWRYLDDGRQSAPHRVALWEKLLAALDKDKLPERYFVFGISTMAPMYL
                     QLLHKLSEHCDVFVFALNPSGMHWGNVIEAAQILKGGGDPDLTQAGHPLLASLGKQGR
                     DFFDFLNEMEIEGETPVFEEGGRDTLLHALQTDIQNLKMPSENVGSVNTGDGSIRIVS
                     AHSPLRELQILKDKLLKILHEHPDWQPHDIAVLTPNIEPYTPFIEAVFGQAQPGAQAL
                     PYSVSDVKISRRQPFFYALECVSDLLESRFEVGKVLALLETAPVLRRFGLTGDDLPLL
                     HDMVADLNVRWGLDGEMRGGTDQLFTWKQAADRMILGWMLPEGGNPMWQDVSAWYADV
                     NQTALFGRFAAFLETLSDIARIWRQPATVGEWVARCRDLLETLFQAGPDDQKAVQNLE
                     NEWVKWQEESTLAGFFGQLPQQTVIRHIRRFLDSESEAGFLRGGITFCSMVPMRSLPF
                     KVICLLGLNDGDFPRNTKAAVFDLVAKHPAKGDRARRDDDRYLFLEALISAREILYLS
                     YIGRDIRKDEELAPSSLLGELIDTVAAMTGTNSRQLAQNWIEQHPLQAFSRRYFQEGG
                     RSDGIFGTRTDYAAALGQTPEPPRPFFDQPVENAEPVAEIGQDEFIRFWRNPVKVWLQ
                     QQLAWSEPHIGEAWEPAEPFDPQHADQIAETYIEARREGQDFAQTAARIGAESLLPSG
                     ELGRLWQQDFQTAAKQIDTAVLNSPKLPPFPYAIPSDGQILKGSLGNLYRCGQVFYAY
                     GKPNAPQRVAFLLEHLIFCAVMPSEAETRQTFIVQSGETEVLAEIAQDRALQLLSEWM
                     AFFNIGQNRPLPFFAKTSLAAAEAFAQKQDWEAALKKAQTAYYGSKVSKGQKDYTEVA
                     LVFGNASQNPLEQPLFENLARLLADTLAAAEKREGTGEA"
     gene            complement(48110..49519)
                     /locus_tag="WNA25_000237"
     CDS             complement(48110..49519)
                     /locus_tag="WNA25_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFA9823089.1"
                     /translation="MDTQIKTEAGQQNNRHYLSVWRWHFYAGLLVMPFLTLLAVTGLG
                     MLLFANITGKEGERIHVFPQAVVQPLSVQAEAARGAINPETSSVVQYIAPRADDMVAV
                     FRVNNEGKATMVAVDPYTAKVVNTMPRNQGWYHTMDEIHGDMMLGAAGDYLLETAASL
                     TIIMVVSGLYLWWAKQRGIKAMLLPPKGRARSWWRNLHGAFGTWVSLILLLFCLSGIA
                     WAGIWGGKFVQAWSQFPAGKWGVEPNPVSVVPTHGEVLNDGKVKEVPWILELTPMPVS
                     GTTVGENGINPSEPMTLETVDRFAREIGFKGRYQLNLPKGEDGVWTLSQDSMSYDMIS
                     PFADRTVHIDQYSGKILADIRFDDYNPFGKFMAASIALHMGTLGWWSVLANVVFCLAV
                     IFIGISGCVMWWKRRPSGVAGIVPPAQKIKLPVWWAMALPLLLIALLFPTALLAIAVI
                     WLLDTLLLSRIPVLRKWFK"
     gene            complement(49863..51182)
                     /gene="ccoP"
                     /locus_tag="WNA25_000238"
     CDS             complement(49863..51182)
                     /gene="ccoP"
                     /locus_tag="WNA25_000238"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HFA9823090.1"
                     /translation="MNATSQFTSNFWNIYIAVIVLLSFIALAWLLLSQNVVKRPKKGE
                     EVQTTGHEWDGIAEYDNPLPRWWFWLCVLTWLFGIGYLVMYPGVGDYKGLLKWTSHNQ
                     YEKEVKKADEQYGKLYAKFADMPIEKVAKDPQAKQIAQNLFNTYCIQCHGSDAKGSKG
                     FPNLTDSDWLWGGDPDKIHETIEKGRVATMPAWGPALGEEGVKDVAHYVMSLSKPKGQ
                     YDEERAARGQALFSGPPANCFTCHGDKGQGIQGLGPNLTDDVWLWGGTQKSIIETITN
                     GRSSQMPAWGHFLDKDKLHIMTAYVWGLSNKDGKAPVKKAEPAPAAEPAPSAPAEAAQ
                     AASEAKPAAAEPKAEEKAAPAAKADGKQVYETVCAACHGNAIPGIPHVGTKADWADRI
                     KKGKDTLHKHAIEGFNTMPAKGGRGDLSDDEVKAAVDYMVNQSGGKF"
     gene            complement(51206..51376)
                     /locus_tag="WNA25_000239"
     CDS             complement(51206..51376)
                     /locus_tag="WNA25_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HFA9823091.1"
                     /translation="MDINGIRALFTVWIFICFLLVLYIVFNRRNKKNYDDAANSIFAE
                     NQDAQDKKSENR"
     gene            complement(51381..51992)
                     /gene="ccoO"
                     /locus_tag="WNA25_000240"
     CDS             complement(51381..51992)
                     /gene="ccoO"
                     /locus_tag="WNA25_000240"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HFA9823092.1"
                     /translation="MKLQQLAEEKIGVLIVFTLLVVSVGLLIEVVPLAFTKAATQPAP
                     GVKPYNALQVAGRDIYIREGCYNCHSQMIRPFRAETERYGHYSVAGESVYDHPFQWGS
                     KRTGPDLARVGGRYSDEWHRIHLLNPRDVVPESNMPAFPWLARNKVDVDATVANMKAL
                     RKVGTPYSDEEIAKAPEALANKSELDAVVAYLQGLGLALKNVR"
     gene            complement(52019..53452)
                     /gene="ccoN"
                     /locus_tag="WNA25_000241"
     CDS             complement(52019..53452)
                     /gene="ccoN"
                     /locus_tag="WNA25_000241"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HFA9823093.1"
                     /translation="MDTQTYNYKVVRQFAIMTVVWGIVGMLVGVIVAAQLFAPALDLS
                     NIGPWFHFGRLRPLHTNAVIFAFGGCGLIGTSYYVVQRTCNTRLFGGWLPAFTFWGWQ
                     AVIVAAVVSFPMGWTQGKEYAELEWPIDILITLVWVAYAIVFFGTIAKRKVKHIYVAN
                     WFYGGFILAVALLHIVNNISIPAGLMKSYPVYSGAIDAMVQWWYGHNAVGFFLTAGFL
                     GMMYYFVPKQAARPVYSYRLSVVHFWALIFTYMWAGPHHLHYTALPDWTQSLGMVLSL
                     ILFAPSWGGMINGIMTLSGAWDKLRTDPILKFLIVSLSFYGMSTFEGPMMSIKTVNAL
                     SHYTDWTVAHVHAGALGWVGFVTIGSVYYMIPRLFGKEQMHSTKLVEAHFWIATIGVV
                     LYIAAMWIAGVMQGLMWSSLNDDGTLTYSFVESVKRTMPYYMIRFAGGLLYLSGMCIM
                     AYNVYRTAIGGKAVDAEIPAVSQTQHH"
     gene            53778..55526
                     /locus_tag="WNA25_000242"
     CDS             53778..55526
                     /locus_tag="WNA25_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HFA9823094.1"
                     /translation="MIHYKIAPSPLDHEWHILLKFTQDNDFPIEISLPNWVPGSYLIR
                     DFSRHITSIHASCNGTSMPLEQIAKNRWHTAAVRGEWQIRYTVYAFDLSVRGSFLTTE
                     RGFFDGSCLFLKVEGTETLPHRLELTGIPPEWRIATTLPETGRLVFQTASYDELIDRP
                     VEMGLIEFLDFEAAGIPHTIALNGIYPDFDRDRLVSDIKKICETELAMFSSPAPFEKY
                     LFLLHVGDHIYGGLEHTDSTALLADRHSLPPYGMTDADDAYTTLLGLFSHEYFHAWNV
                     KSIKPAAFAPYDLDKENYTEQLWAFEGITSYYDDLFLARSRTISPEFYLNLLAQGITR
                     VQQTRGRLRQTLAESSFTAWNKFYKPDENSPNAIVSYYQKGALAALCLDLIIRNRSNG
                     RHSLDTVMDKLYREWRDTHSGIPEKHWQIRCQEITGLDLTDFFQTALYSTEDLPLAEC
                     LATTGVKLTFLPLPRQHGGGYAEHIFPIPPTGDFGARFKQNADHIVLTHVFNGGSAES
                     AALCPQDKIIALDGYACTDFAAQWARYHVRAKINIHFFRAGILRQTVLTVQATAADTA
                     ILHITDRNLLENWLFG"
     gene            55591..56019
                     /locus_tag="WNA25_000243"
     CDS             55591..56019
                     /locus_tag="WNA25_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family phosphatase"
                     /protein_id="HFA9823095.1"
                     /translation="MAILKLDEHLYISPQLTKADAEQIAQLGIKTVICNRPDREEESQ
                     PDFAQIKQWLEQAGVTGFHHQPVTARDIQKHDVETFRQLIGQAEYPVLAYCRTGTRCS
                     LLWGFRRAAEGMPVDEIIRRAQAAGVNLENFRERLDNARV"
     gene            complement(56520..56954)
                     /locus_tag="WNA25_000244"
     CDS             complement(56520..56954)
                     /locus_tag="WNA25_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HFA9823096.1"
                     /translation="MAFGSMNSSDDSPMSDINVTPLVDVMLVLLIVFMITMPVLTHSI
                     PLELPTASEQANKQDKQPKDPLRLTIDANGGYYVGGDSASKVEIGEVESRLKAAKEQN
                     ENVIVAIAADKAVEYDYVNKALEAARQAGITKIGFVTETKAQ"
     gene            complement(56957..57619)
                     /gene="exbB"
                     /locus_tag="WNA25_000245"
     CDS             complement(56957..57619)
                     /gene="exbB"
                     /locus_tag="WNA25_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HFA9823097.1"
                     /translation="MNLKLVFESGDPVLIGVFVLMLLMSTVTWCLVVLRCIKLYRARK
                     GNAAVKRHMRDTLSLNDAVEKVRAVDAPLSKLAQEALQSYRNYRRNEASELAQALPLN
                     EYLVIQIRNSMAQIMRRFDYGMTALASIGATAPFIGLFGTVWGIYHALINIGQSGQMS
                     IAAVAGPIGEALVATAAGLFVAIPAVLAYNFLNRGTKILTQDLDAMAHDLHVRLLNQK
                     DS"
     gene            complement(57685..58536)
                     /locus_tag="WNA25_000246"
     CDS             complement(57685..58536)
                     /locus_tag="WNA25_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HFA9823098.1"
                     /translation="MDKERILTPAVVFSVALLHLAIVALLWQAHKLPVIESGNVIEFV
                     DLGDFGGGGGAPEGAGAPAAPEPQPAPDPPKPVEPPKPVLKPAVTKKADADIQQPKEK
                     PKPEEKPKPEPEPEAKPAPKPAEKPAEKPSEKPAEHSGNASAKAGSEQGNGEGKGTGT
                     KGDGTGRGEGSGKGSGGAKGEHGEGAGGSGGGTGVGSSKGNPLRANGSIPRPAYPALS
                     MENDEQGMVVLSVLVSPGGHVESVKVVKSSGFSRLDNAARKAAQNGHFQANAWTEFKV
                     PVKFELN"
     gene            58792..59430
                     /locus_tag="WNA25_000247"
     CDS             58792..59430
                     /locus_tag="WNA25_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HFA9823099.1"
                     /translation="MFQNFDLGVFLLAVLPVLLAITVREVARGYTARYWGDNTAEQYG
                     RLTLNPLPHIDLVGTIIVPLLTLMFTPFLFGWARPIPIDSRNFRNPRLAWRCIAASGP
                     LSNLAMAVLWGVVLVLTPYAGGAYQMPLAQMANYGILINAILFALNIIPILPWDGGIF
                     IDTFLPAKYSQAFRKIEPYGTWIILLLMLTGVLGAFIAPIVRLVIAFVQMFV"
     gene            complement(59502..60143)
                     /locus_tag="WNA25_000248"
     CDS             complement(59502..60143)
                     /locus_tag="WNA25_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrxB family glutaredoxin"
                     /protein_id="HFA9823100.1"
                     /translation="MKLYIYDHCPFCVRARMAAGLFGADVEEVVLANDDEATPIGMIG
                     AKQVPVLQKEDGSFMGESLDIVCHFDREGRLKDEVRPEIQAWLDKVGGYNNKLVQPRL
                     IKIGLPEFVTPEAVKYFTDKKEKSIGSFSANLNKTAQYLERINADLQELENLMDGTSD
                     GINGGIGMEDILVFPVLRNLTVVRGIAFPRKTMDYLIGMSEKSGVPLYFDRAL"
     gene            60286..62499
                     /locus_tag="WNA25_000249"
     CDS             60286..62499
                     /locus_tag="WNA25_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFA9823101.1"
                     /translation="MTAISPIQDTQSATLQELREWFDSYCAALPDNDKNLIGTAWSLA
                     QEHYPADAATPYGEPLPDHFLGAAQMVDELDLLPDAVAATLLADIGRYVPDWNLLVSE
                     RCNSTVAELVKGVDEVQKLTHFARVDSLATSEERAQQAETMRKMLLAMVTDIRVVLIK
                     LAMRTRTLQFLSNAPDSPEKRAVAKETLDIFAPLANRLGVWQLKWQLEDLGFRHQEPE
                     KYREIALLLDEKRTERLEYIENFLDILRTELKKYNIHFEVAGRPKHIYSIYKKMVKKK
                     LSFDGLFDIRAVRILVDTVPECYTTLGIVHSLWQPIPGEFDDYIANPKGNGYKSLHTV
                     IVGPEDKGVEVQIRTFDMHQFNEFGVAAHWRYKEGGKGDSAYEQKIAWLRQLLDWREN
                     MAESGKEDLAAAFKTELFNDTIYVLTPHGKVLSLPTGATPIDFAYALHSSIGDRCRGA
                     KVEGQIVPLSTPLENGQRVEIITAKEGHPSVNWLYEGWVKSGKAIGKIRAYIRQQNAD
                     TVREEGRVQLDKQLAKLTPKPNLQELAENLGYKKPEDLYTAVGQGEISNRAIQKACGT
                     LNEPPPVPVSATTIVKQSKIKKGGKTGVLIDGEDGLMTTLAKCCKPAPPDDIAGFVTR
                     ERGISVHRKTCPSFRHLAEHAPEKVLDASWAALQEGQVFAVDIEIRAQDRSGLLRDVS
                     DALARHKLNVTAVQTQSRDLEASMRFTLEVKQVNDLPRVLAGLGDVKGVLSVTRL"
     gene            complement(62563..62940)
                     /locus_tag="WNA25_000250"
     CDS             complement(62563..62940)
                     /locus_tag="WNA25_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HFA9823102.1"
                     /translation="MCEFKDFRRNIPCFEEYDENSFIGKWYDDGVWDDEEYWKLENDL
                     IEVRRKYPYPMDIPRDIVIGIGTIIEFLMVPNWKLFTIKSSPWLPDSVGIHERYERFT
                     TMLRYIFTEKDIVNVRFDYYNKK"
     gene            complement(62946..63002)
                     /locus_tag="WNA25_000251"
                     /pseudo
     CDS             complement(62946..63002)
                     /locus_tag="WNA25_000251"
                     /inference="COORDINATES: protein motif:HMM:NF026864.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(63009..63464)
                     /locus_tag="WNA25_000252"
     CDS             complement(63009..63464)
                     /locus_tag="WNA25_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823103.1"
                     /translation="MVDAVVKTPEFLPFTSVGIFRFGADITQYKNILETFMYEPPDEF
                     GTEYYESPDSNLLISVKKNKIISIFCYQELYFMGVNIIGLNFEDFKQLFHHPKSYGVD
                     KYYLSNESYPTYVYEFDEIGVQAWEAKGKIVTIIAGGKDNYSTEPYYDE"
     gene            complement(63445..>63717)
                     /locus_tag="WNA25_000253"
     CDS             complement(63445..>63717)
                     /locus_tag="WNA25_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pretoxin"
                     /protein_id="HFA9823104.1"
                     /translation="YFVKGNQAETEGVWVHNSCPPKRTGSSKNEKHGDGGRSQISAES
                     KIAELTNKIIPGMSKNERLKIKQKIKNIAKNANRKTKGEEHGRRGR"
BASE COUNT        15014 a        17944 c        15970 g        14789 t
ORIGIN      
        1 caaatgggtt aaaccagtat ccatacatca gcttttttat catcctactt tttattcatc
       61 cgatcgggca gacagatttc aaagatgaaa agcctattct attcacaccc atttgatgtc
      121 atttccacac ggacgaacaa ataaattccc ataaaaaaac ggagcagata cctgccccgt
      181 tttttaattt aatccgaatt tagaattttg caccggattg gtttgccata tagtcaacag
      241 ccgctttgac ttcgtcatcg ctcaaacctg cattgccgcc tttggccggc atcgcgttaa
      301 agccttcaag ggcatgtttg tgcaaggttt ctttgccttt tttgatacgc ggtgcccaat
      361 cgtctttttt gcctatgccg ggaataccgg gaatcgaacc gccgtggcac acctgacagg
      421 ttgcttcgaa gactttttta ccgtcaacgc cgaccgcagg ggctgccgca cccttgtctt
      481 ctgccttcgc ttctgccgga gctgcactat cggcaggagc agaagctgtt cctgaagcgg
      541 cattgtcggc aggcgcagcc tcatcaggat tcgggaaaga accgccgctt ttattcgcca
      601 tgtaggtaat cgcccgtttg agttcctgat cggtcaggtc tgccgcaccg cctttggcag
      661 gcatggcgtt aaagccgttc agcgcgtgtt ggaacaaggt atcgaagcct tgcgcgatac
      721 gcggcgccca gtcgccgttg tgttccagtt tcggagcgtt cggcacattg ctgtccgccg
      781 cgtggcattg gatacagatt ttgccgaaaa tctgttcgcc ttggcgttcg ccgacgggga
      841 tgccgtcacc catcgtcaat tgtccgacag gctggatgcg ggtctgcgtt gccgcttccg
      901 tagtggcatc gacatcgccg aacgatccac tgcccgccag cttaatcagg aaataaagga
      961 ctgcaataac aataacgata ccgctcacaa gggtaaacag tgcagagcct tgggctttgt
     1021 tgtcgcggag ttgtttcatt tggtaggcct cgccgtcagg ttaggttgtg ctgtagatta
     1081 tagtttggcg tgttaaacac agttaacaat attttgctgg attatactga attcacatgg
     1141 tctttccaat cgctatcatt gaaaatatga aaaaatttgc caatggtatc tgtacaaaac
     1201 aaataatccg ttgaaaatac cggattatct ccaagaaaac tccccttgtg ccgccatacg
     1261 ccgcctgccg gcgcaagata acctttgcca atttgcagaa tttacgttaa ccttacgttt
     1321 tccgcaccca tagctcagtt ggaagagtgt cagtttccga agctggaggt cacaggttcg
     1381 atccctgttg ggtgcgccaa ttataaagag gccgtctgaa agataaatat ttttcagacg
     1441 gccttttgac ttacttcaaa ttcttatttc aacacttcgg caagcgcatc ggcgacataa
     1501 gtgatatttg atgtattcag cccggcgacg cacatcctgc cggaatccag caggtaaacg
     1561 gcaaattcgt cgcgcaacct gcggacttgc cccacgctca accccgtgta gccgaacatt
     1621 ccgcgctgtt tgatgaaata agtgaaatcg cggtcgggga ttcgggcggt caatacgccg
     1681 taaagtttct gccgcatcgc acggatgcgg tcgcgcatca tataaacttc gttttgccac
     1741 aaggcgtaaa gttcggagct gttcatcacg tcggcggcga tatacgcgcc gtgcgcgggc
     1801 gggctggagt agatgcggcg gacggtgaat ttgagttgtc cgaacaccaa atccgcttct
     1861 tctttattgg ggcaaaccac gcttaagccg ccgacgcgtt cgccgtagag cgacaggttt
     1921 tttgaaaacg aattgctgac aaacagcggc aaatccattt ccaccgcttt gcggacggcg
     1981 taggcatcgc tgtccaaatc gccgccgaag ccttggtagg cgatgtccat aaacggaatc
     2041 agtttgcgcg ttttgatgat ctgcaacact tcgtcccatt gccgttccga catatccacg
     2101 ccggtcgggt tgtggcagca gggatggagg atgaggacgc tgttttcggg cagggtgttg
     2161 aaaaacgcgg tcatttcgtc gaatttcacg ccgacagtgg cagggtcgta atatgggtaa
     2221 gtgccgacct cgaaacctgc gccttcaaaa atgccgcgat ggttgtccca agtcggatcg
     2281 ctgacgtagg cgcgcgcttc gggaaaccag cggtgcagga aatccgcccc gactttgagc
     2341 gcgcccgagc cgcccagagt ctgtacggta acgatgcgtc cctgcgcgag cgcggggttg
     2401 cctttgccga acaacaaatg ctgcaccgcg ctgcggtaaa cgtccaaacc ttccatcggc
     2461 aggtagggcg acggcgcagg cacagcggtg cgggcggttt cggcacggct tacggattcc
     2521 aatacgggca ttctgccttc gtcgtcgaaa taaatgccta tgctcaaatt gactttttcg
     2581 gggcgcgggt cgtttttgaa ggtttcgacc aaactcaaaa tcgggtcgcc gggatagtat
     2641 tcgatgtgtc ggtacatagt ccttaccttt tgcttttcaa aggattttct ttttcaacag
     2701 tacgccgctt tcgatatggt gcgtaaacgg gaattggtcg aacagggcgg cacgtccgac
     2761 cgtatgggtt tccgtcaagg tgtccaaatt ggcgcgcagc gtttcgggat tgcaggaaat
     2821 gtagatgatg ttgtcgaact gcgacaccag cttcaaagtt tcctcatcga tgccggcacg
     2881 cggcggatcg acgaaaatag tggaaaatgc gtaatccgtc aaaacaatac cgctctcctt
     2941 aaggcgtgta aactcacgtt ttccggtata ggcttcggta aactcctcag cagacaggcg
     3001 ggcgattttg atgttgccta tccagtttgc ctcaatattc cattgcgccg cactgacgga
     3061 ggttttggag atttcggttg ccaaaacctg tcggaaatat cgggacagcg gcagggtgaa
     3121 attgccgttg ccgcaataca gttcgagcag gtcgccgccc aagccttccg ccgcgcggca
     3181 cgcccattcg agcattttcc ggcatacagc ggcattcggc tgggtaaaac tgccttcgat
     3241 ttggcggtag tggaaatcgc ggtcgccgac gcgcagggtt tccgtcacat agtcctgttt
     3301 taagactatt ttctgccccc tgctccgccc gataacggaa atatccaact gttgctgtaa
     3361 cgcttgcgcc gccttcatcc actcatcatc aagccttttg tggtaaatca tggtaaccag
     3421 catttccccg ctgagcgtgg acagaaattc gacggcatac caatggtttc tcagttcggc
     3481 agattgggcg gcaacggcaa tcaattcggg catcaggcag ttgactgcct ctgaagcagc
     3541 atcgaaacgg tcgcaacgga tcaggcttgc cccgctagcc ttctgccctt tttcaaacat
     3601 ggcgtaaaac atttcaccgc cttcgtgcca aatgcggaat tcggcgcgca tgcggtagtg
     3661 tttgtccgga gattcgtaca cttcccactc aggaacatcc aaacctgcaa aaagggtttt
     3721 aaggtagtct tttttacctt gaagctgctg cgtgtagtca ttcatatcgc tatcctgaaa
     3781 aatcaggccg gattataagc cgattttcct cgccgcacgc aaactctttg actgccgccc
     3841 cttcaatgac ggacgggctt ttgtgctaaa atccgccatc tttccacact ataccgataa
     3901 agggaaaaat catggcaggc aacactttcg gacaaatctt caccgtgacc accttcggcg
     3961 aaagccacgg cgcgggtttg ggctgcatca tcgacggctg cccgcccggc ttggaattaa
     4021 gcgaagcgga tatccaattt gacctcgacc ggcgcaaacc cggcaccagc cgccacgtta
     4081 cccaacgccg cgaagccgac caagtcgaaa tcctctccgg cgtattcgaa ggcaaaacca
     4141 ccggcacacc catcgccctc ctaatccgca acaccgacca gcgcagcaaa gactacggca
     4201 acatcgccac cgccttccgc cccggacacg ccgactatac ctattggcac aaatacggta
     4261 cgcgcgacta ccggggcggc ggcaggagtt ccgcccgcga aaccgcagcc cgcgttgccg
     4321 ccggagccgt tgccaaaaaa tggctgaaag aaaaattcgg cacggaaatc accgcctacg
     4381 ttacccaagt cggcgaaaaa aaaatccggt ttgaaggcag cgaacacatt tcccaaaatc
     4441 ctttttttgc cgccaaccaa agccaaatcg ccgaactgga acactatatg gacggtgtgc
     4501 gcaaatcctt ggattccgtc ggcgcgaaac tgcatatcga agccgccaac gtccccgtcg
     4561 gtttgggcga acccgttttc gaccgcctcg atgccgaaat cgcctacgcg atgatgggca
     4621 tcaacgccgt caaaggcgtg gaaatcggcg caggttttga cagcgtaacg caacgcggca
     4681 gcgaacacgg cgacgaactg accccgcaag gcttcctgtc caaccattcg ggcggcatcc
     4741 tcggcggcat cagcaccggg caagacatct gcgtcaatat cgccatcaaa cccaccagct
     4801 ccatcgccac accgcgccga agcatcgaca ttcacggcaa ccccgtcgaa ctcgccaccc
     4861 gcggcaggca cgatccctgc gtcggcttgc gcgccgcgcc gattgccgaa gctatgctcg
     4921 ccttggtttt aatcgaccac gccctgcgcc atcgtgcgca aaatgcggat gttgccgcgg
     4981 atacgcccga tatttcccgc tcggacaagt aaaaagattt agccaaaaca cgggctttat
     5041 actaaaatac cgaccattgc cgtgccgtct gaacgcacga ttcttagagg aatacaaccg
     5101 aaatgacaca agaaaccgct ttgggcgcgg cactgaaatc cgccgtccaa actatgagca
     5161 aaaagaaaca gaccgaaatg atcgccgacc acatctacgg caaatacgat gtattcaaac
     5221 gcttcaaacc gttggcgctc ggcatcgatc aggatttgat tgccgctttg ccgcaatacg
     5281 attccgcact gattgcacgc gtcctcgcca accactgccg ccgtccgcgc tatctgaaag
     5341 ccttggcgcg cggcggcaaa cgtttcgatt tgaacaaccg tttcaaaggc gaagttaccc
     5401 ccgaagaaca ggcgattgcg caaaaccatc ctttcgtgca gcaggctttg caacagcagt
     5461 ccgcccaagc tgccgccgaa acgccgtctg ttgaagccga agcagccgaa tcttccgcag
     5521 cagaataaat ccccaaacga aatgccgtct gaaaaccgat ttggtttcag acggcatttt
     5581 ttcgtatgcg gcaatcacgg ctcaaatatc gagttgcgcc gtatcgcctt cgcgttccat
     5641 ccaagcgcgg cgggcggcgg cttcgccttt gcccatcagt ttgacgaaga tgtcgcgcgt
     5701 ctcgtcatcc gcgccttcgg ggatttgcac ctgcaacagg cggcgggtgt cggggtgcat
     5761 ggtggtgtct ttgagctggt cggggttcat ctcgcccaag cctttgaaac ggctgatgga
     5821 ataggcggtt tctttaacgc cttctttttg cagccgctct aaaatgccgt caagttcgtt
     5881 ttggtcgagg gcgtagaatt tgcgggcggg tttgcttttg ccttgtgcgt tgacatcgac
     5941 gcggaacagc ggcggctggg cgacgtagat gtgtccgtcg gcgaccagtt tggggaagtg
     6001 gcggtagaac agggtcaaca gcaaaacctg aatatgcgaa ccgtccacgt cggcatcgga
     6061 caagatggct attttgccgt agcgcaggcc gcttaagtcg ggatggtcgt tgatggcgtg
     6121 cggatcgaca ccgatggcga cggaaatgtc gtggatttcg gcgttgccga aaagctggtc
     6181 ggggtggact tcaaagctat tgagcacttt gccgcgcagg ggcaggatgg cttgtgtggc
     6241 tttgtcgcgg gcgagtttgg cggagccgcc ggcggaatcg ccttcgacga ggaagagttc
     6301 gttttcgcgg atgtcttcgc tttcacagtc ggtcagctta ccgggcagga tggcgacgcc
     6361 gctgcctttt ttcttttcga tttttttaac cgaacgtatc cgtgcctgtg cctgacggat
     6421 ggcgagttcg gcgatttttt tgccgaagtc cacgttttgg ttcagccaca attccaaagg
     6481 atcgcccgat acggcggcga caagtttcag cgcgtcgcgg ttggtcagct tgtctttggt
     6541 ctgaccttgg aattgcgggt cgaggacgcg ggcggagagg acgaaggcgg tttttccgaa
     6601 cacgtcgtcg ctttgcactt taacgccgcg cggtaagagg ttgtgcaggt tgatgaaatt
     6661 gttgacggcg ttgaacacgg cttgtttcaa gcctgcttcg tgcgttccgc ccagcgaggt
     6721 cgggataagg ttgacgtagc tttcgttggc gcacgagcct tcttccagcc aagtcaaagc
     6781 aaacgcggca ccttcgccaa tgctgaaatc gccgttgtgg ccgtctgaaa tgtagttttc
     6841 gcaggagaac agcggcacgg cttcctgtgc gtcggcaatc aggtcggtca gatagctttt
     6901 cagaccgtcg gggtaatgcc aggtttgggt gtgcgcttcg tcttcgcctt tgaccggacg
     6961 ggtcagggaa acgcgcacgc ccggcagcag cacggctttg gcgcgcaata gacgctcgag
     7021 ttcgggaatg ctgtaattcg ggctttcaaa atatttgccg tccggccaca cgcgcacttc
     7081 ggtgccgctg tctttgacgg cgcatttgcc cacttgtgcc aacggttcga ccacatcgcc
     7141 gccggcaaac acgatgcggt ggactttgcc gtctcgtttg acactgactt caagtcgggt
     7201 ggaaagcgcg ttggtaacgg atacgcccac accgtgcagg ccgcctgaaa aggcatacgc
     7261 actgccgccg tcttttttgt tgaacttgcc gcccgcgtgc aggcgggtaa acacgagttc
     7321 gactacgggt acgccttctt cagggtgcag gccgacggga atgccgcgcc cgttgtcgcg
     7381 cacggaaagc gaaccgtctt catgaatttg cacgtcgatt tcagtggcga aaccgcccaa
     7441 cgcctcgtcc gccgcgttgt cgatgacttc ttggcagata tgtgtcgggc tgtcggtgcg
     7501 ggtgtacatg ccgggacgtt ctttgaccgg ctccaagcct ttgaggacgg tgatgctgga
     7561 ttcgctgtat tggttgtttt tagccatagg aataatctga aagtaagaaa aacaacgctt
     7621 tcagacggcc tgaaagcgtt gcgttccgtt gttttaccgg ttgccggaag gttggcgggc
     7681 ggcaaagtct tcataacttt ccataccgcg caggaagcgg gaagagagtt ctttcaacgc
     7741 ccccaaataa acgtcgcgtt tgaaatcaat cacttcgtca acgggcgccc aatattgatg
     7801 ccaacgccag ccgtcaaatt cggggtggcg ggtggcgcgc aggttgacat cgcaatcccg
     7861 gccggtcagg cgcaggagat accaaatctg cttctgcccg cgataagagc cgcgccattc
     7921 gcggcgcacc cagttgttcg gcacgtcgta acgcagccag tcgcgcgtcc gcccgacgat
     7981 tttgacgtgt tgcggcagaa gtccgacttc ttcgtaaagt tcgcggtaca tcgcggtttc
     8041 ggggctttcg ccgggcttga tgccgccttg aggaaactgc catgaatgtt cgcgcacgcg
     8101 cttaccccaa aagacttcgt tacgttcgtt aattaggata ataccgacat tggggcgata
     8161 gccttccctg tccaacacgg tgtcgccctc cgttaaattc aatcttggga ttttcccaca
     8221 aatcggacgg ttttgacaaa tcggacggta tggttcaagc atgtcgaaac acggacggat
     8281 tcgggaaaat atcttaaatt tggtttacaa taatacgttt caaattaatt ccggaatcag
     8341 actatgttag atatccaatt gctccgcagc aacaccgccg ccgttgccga acggcttgca
     8401 cggcgcggtt atgactttga taccgcgcgt tttgacgcac tggaagaacg acgcaagtcc
     8461 gttcaggtga aaaccgaaga actacaagcc tcgcgcaaca gcatttccaa acaaatcggc
     8521 gcactgaaag gtcagggcaa acatgaagag gcgcaggcgg ccatggatca agtcgcccaa
     8581 atcaaaaccg atttggaaca ggctgccgcc gatttggatg ccgttcaaaa agaattggac
     8641 gcatggttgt tgagcatacc taacctgccg cacgaaagcg tacctcccgg taaagacgaa
     8701 accgaaaacg tcgaagtccg caaagtcggt acgccgcgcg aatttgattt tgaaatcaaa
     8761 gatcatgtcg atttgggcga gcctttgggc ttggatttcg aaggcggcgc gaaactctcc
     8821 ggcgcacgat ttaccgtgat gcgcggacaa atcgcccgtc tgcaccgcgc cttggcgcaa
     8881 ttcatgctcg atacgcacac gctgcaacac ggctacaccg agcattacac gccttatatc
     8941 gttgacgata cgacgctgca aggcacaggt cagctgccca aatttgcaga agatttgttc
     9001 cacgttaccc gcggcggcga tgaaaccaaa accacgcaat atctgattcc gacagccgaa
     9061 gttaccctga ccaataccgt tgccggcagc attatcccgt ccgaacaact gccgctgaag
     9121 ctgaccgcgc attcgccctg tttccgcagt gaagcgggat catacggcaa agacacgcgc
     9181 ggcctaatcc gccagcacca gttcgacaaa gtagaaatgg tacaaatcgt tcatcccgaa
     9241 aaatcatacg gcgcgctgga agaaatggtc ggccatgccg aaaacatcct gaaggctttg
     9301 gaactgccct accgcgtgat taccctgtgt accggcgaca tgggtttcag cgcggccaaa
     9361 acgtatgact tggaagtttg ggttcccgca caaaatacct accgcgaaat ctcaagctgc
     9421 tccaactgcg aagatttcca agcacgccgc atgaaggcgc gtttcaaaga cgaaaacggc
     9481 aaaaaccgct tggtacatac cttaaacggc tccggtttgg cagtcggtcg cactttggtc
     9541 gcggttttgg aaaaccatca aaacgccgac ggcagcatca atatccccgc cgcactgcaa
     9601 ccgtatatgg gcggtgttac caagttggaa gtgaaataag ccttaaccct gaacataaat
     9661 gccgtctgaa acctgtttca gacggcattt cctttaaact cttaaaacac atcaaccgtc
     9721 ggcacgaacc gcattgccgc aatcgccggt ctgtccgacc tcgcggatat tggacagcgt
     9781 aacttccgaa atattaccca acgcctcttc cgtcaaaaat gcctgatgtc cggtaaacag
     9841 tacgttatga caagacgaca ggcggcggaa cacgtcgtcg gtaatcacat cgttggattt
     9901 gtcttcaaaa aacagctcgc gctcgttctc gtacacatcc atgcccaatg caccgatttt
     9961 ccggcatttc aacgcctcga tggcggcggc gctgtcaatc agcccgcccc ggctggtgtt
    10021 gataatcatc acgccgtctt tcattttgtc gaacgccgct tcgttcagca tatagtggtt
    10081 ttccggcgtg gcgggacaat gcagcgtgat gatgtccgat cgggcataca gctcgtccaa
    10141 atccacatac ctgccgccga gtttttccac ttcggggttg caaaacggat cgtaagccag
    10201 caggttcata ccgaaaccct ttaaaatccg catcgttgcg ataccgattt tccccgtgcc
    10261 gataacgccc gccgttttgc cgtacatatt gaagccggtc agaccttcca gcgaaaaatt
    10321 cgcatcgcgg gtacgctggt aggctttgtg gatacggcgg ttcaaagtca gcatcagacc
    10381 gaccgtatgt tccgcaaccg attcgggcga ataggcaggc acgcgcacga ctttcaagcc
    10441 caactcttca gccgccttca aatccacatt attgaagccg gcacaacgca acgccacagt
    10501 tttcacgcca atttgcgcca atttttccaa cacgggccgg ctggcgtcgt cgtttacaaa
    10561 aatacaggcc gcttccgcgc cttccgccat tttcgccgtt ttcgcatcca gcatgaaatc
    10621 aaaaaactcc agctcgaggc cgaaatgccg gttggcgcgg gtaaaatgtt cgcggtcata
    10681 gcttttcgta ccgtaaatcg caatcttcat caatatgtcc agttgtcgtc tatggttgag
    10741 aaacggcata atacaccgaa ttcaaacaaa tcagtagaat atggcggatt aaaattgatt
    10801 tcatgcacgg catttccatt tcaaaacaca aaactcaatc gcccatcgcc gccagaagct
    10861 ccgcctgatg ttcggcaatc agggcatcgg tgatttctgc caaatcgccg tccatcacaa
    10921 aatccagctt gtgcagggtg aggttgatgc ggtggtctgt taccctgcct tggggatagt
    10981 tgtaggtgcg gatgcgttcg ctgcggtcgc cgctgccgat gagggatttg cgttcggcgg
    11041 cttctttggc ttggacttcg cgtttttgcg cgtcgttcag gcgggcggcg aggactttca
    11101 tcgcctgcgc tttgttggcg tgttggctgc ggccgtcttg gcattcgacc accatgcctg
    11161 tcggcaagtg ggtaatgcgg acggcggagt cggttttgtt aatgtgctga ccgcccgcgc
    11221 cggatgcgcg gaaggtatcg gtgcgcaggt cggcggggtt cagttcgatg tcttcgagct
    11281 cgtccgcttc gggcatgacg gcaacggtac aggcggaggt gtggatgcgg ccttggcttt
    11341 cggtggcggg gacgcgctgc acgcggtgtc cgcccgattc aaatttcaga cggctgtacg
    11401 cgccgagtcc gacaatacgg gcgatgactt ctttatagcc gcccaattcg ctttcgttgg
    11461 cggacacgat ttcaacctgc cagcggttgc gttcggcgta gcggctgtac atacgcagca
    11521 aatcgccggc aaacagcgcg gcttcgccgc cgcccgtgcc ggcgcgtatt tcgataaaga
    11581 tgtttttgtc gtcgtcggca tctttgggca gcagcagttt ttgcaattcg gtatcgagtt
    11641 cgtcgatttt ggctttcgcc gcttcgattt cttcggcggc aaagtctttc atttcggggt
    11701 cggacaacat ttcttcggca tccgccaagt cgctttgggc aagccgatag ttttggaata
    11761 cttcgacgac gggggtcagt tcggcgtgtt cgcgcgtgag cttgcggtag ttgtccatat
    11821 cggatgtggc ttcgggttgc cctaaaaggt gggtaacttc ttccaaccgg tcgccgagtt
    11881 gttgcagttt ttctaagata gacggcttca taattcttcc ataacaaacg ccgcctgaat
    11941 ttcagacggc atcaaaacgc ggtcattata atatattttc tgaatattca aaaagataat
    12001 ctagggacgg agcagactgt cggcattgcg ttccgttatc gcggcgattt ccgccgcgcc
    12061 ggtaccgcgt atttccgcag caatttcggc aatccgaaga atgttggcag gcgtattgac
    12121 ggtgttttcc aacatgaacg ggctatccgt ttccaatacg aaatccccgt cgttcaaggc
    12181 tttaagcgta tcgcgcactt tacgcgcgtt cggattgagc agcagcgaac cgatgccgat
    12241 tttgaacccc agttttgtca acacgcgcgc ttcttccgcg ctgcctgaga aagcgtgaac
    12301 gatgccgcct tgagtaaagc ctgtctgttt gacggcggcg gcgatgtcgg cggtggcttt
    12361 gagattatgg ataatcacgc ggcggcgcag ggtttgcgcg atggcaagct ggcggctaaa
    12421 aacttgaatt tgccgttcac gctgcggcgg cgtttgggtt ttgtcgtaaa aatccaagcc
    12481 gatttcgccg acccacgctc gggggtaacg caccaatacg gcttccaaac cggaaaaatc
    12541 ctgttccgca atgccgtctg aaaaccaagg atggatgccc agcgcaatac ggatttgacc
    12601 gtgttcggac ggcatttccg acagttccgc cacgtcctgc caatcctgcg gacaggtagc
    12661 gggaacgata aaccgccaca cgcccgcctg tcgcgcttcg gcaagcacct gcgccagatt
    12721 gccgcgcaaa acggaatcgg caagatggca atgtgtgtcg gtcagcctca tcttcacacc
    12781 agccccaacc gctgcaactc gtgtttcacc ccgtccgcgc cccatccttc ggaaacggca
    12841 agcgtcatca atgcggttgc ggtttccaaa ttgcatttgc cgccgttgac cacgcccgcc
    12901 cgacgcagcg cgtcgccctg tgcgtaaacg gcggcagcgc aaccctgcgg cacttggctg
    12961 atgttaagca acaatttgcc ttgctgcgta aagtcttgca cggcgcggat gaagccttcg
    13021 ccggcggagg tattgccgtg tccgtagctt tgcaggataa gggcttgggc gggaagctgt
    13081 ccgagcccgt cggcaagttc ttggacggca aagccgggga taagcgtgcg gacggcgatt
    13141 tttgcctgcg ggtcgggata acggatttcg aggccgtctg aaacatctgc cgcgtcctga
    13201 ttcgggatgc ggacattgtg ccagccccgg gtttcgtccc actcggcaag tgcgccgaaa
    13261 tgggcattgc cgaaaccggc ggcggtttcc gtgctgattt tgctgctgcc gacggcggga
    13321 tacagtttgc cgtcaaaggc aataagcgtc tgcttcaatc cgagtgggaa tgcggcaacg
    13381 gcagtcacca gattgcgcgg agcgtcgctg ccctctgcgt tgtacggcca ttgcgaaccg
    13441 gtcaggacga tcggtttgtc caaaccttgc agggcgaggg cgaacatatt ggcggtatag
    13501 gcgagtgtgt ccgtgccgtg cagcaccaaa acaccgtcgt actgcggcag cgcggcggca
    13561 atgatgtcca gccaagcgca ccagttttgc agttggacgg cggaagaatc aatcagcggc
    13621 tcacaaacgt gccaatcgaa atccatgccg tctgaaaacg gcgcgagtgc ctgcccgacc
    13681 agcgcggtat cggggcgcaa gcccgcactg ctttgcgtca tgccgatggt gccgccggta
    13741 taaaggacaa aaattttctg tttcatggct cattctgaaa atgcaaagac cgcctgaaac
    13801 gcgaaatgcg tccggacggt cgaaaatccg atttactgct gcttctgtgc cgcttttttc
    13861 gctgcctttt ccgccttgcg tttggcgcgt ttttcgctca attgtgcgcg gtaaagctga
    13921 taatgtcggc gtttgcgcca ccagaaccac gccagcgccg ccgccagcac gcccaatgcg
    13981 atgaagatgc ccgattgcag gctgtgcatt ttcgccatca gccaatcgat gttgtgcgcg
    14041 ccgtactcgc ccaagtaaat ccaaacgggc acggaaatca gcgcggccag cccgtccata
    14101 atcagaaagc gcagatacga tactttgcgg ctgatgccgg cggtaacgaa aacggcagtc
    14161 cgcaaacccg gcaggaaacg ggcgacaaac agaacccagt tgccgtattt gtcgaatttt
    14221 tcctgaacct gcgcgtaacg tttcggcgtc atgatgcgcg caatcggttt gaacttgagg
    14281 attttctgcc cccagatgcg tccggcggca aacatcacgc cgtcgcccgc caacacgccg
    14341 agcataccga ccgcaaacat aatatgcgga ttggtataac ccatacccga aatcacgccg
    14401 cccgttacca aggtcaaatc ttcgggaatc ggcacgccga aaccgcagat gaccaaaacg
    14461 aaaaacacgg ccgcatagcc gtattcgaca aaaaaggctt ccaaaagggc aaacatagcg
    14521 gatattccat tgtcggagat aaaaagtcag aacaagccga aacattttct gcgcgaaacg
    14581 gcattctatc aaagattatg ccgtctgaaa gcggcagggg gggcagattc gcgccccgcc
    14641 ggacgttcag acggcattta aacaggaaaa ccgccgctgt tgaaacggtt ttgcctgatt
    14701 ttgcctaaat gccgccgatg gcggcggcga tacgctccgc cccgtcgcgc gcccaatcgg
    14761 cctgacgcgc ctcgaccatc acgcgcacga cgggttcggt tcccgaagcg cgcaacacga
    14821 cacgcccttt gccttcgagt tctttttcca cttcggccaa cacgtctttc gaagcctcct
    14881 gccatttctg acccttttgg atgcgcacgt taatcatcgt ttgcggatac ggctgccagt
    14941 cggcgcaaac ggtggcgagg tcttggttca ggatttgcag cgccgccaag acttgcagcg
    15001 cggaaataat gccgtcgccg gtgttatgtt tgtccatgca caaaatatgg ccgctggctt
    15061 cgccgccgat gagccagccg cgttggttca gctgttccaa cacatagcgg tcgccgactt
    15121 tggcgcggca gaaatcgacg ccctgctctt tcagggcgat ttccatcgcc atattggtca
    15181 tgaccgttcc gaccacgccg ccgatgttga taccttctcg ggcgcgggct ttggcgatga
    15241 cgtaaatcag gctgtcgccg tcgtaaacct gcctgttttt atcgaccatc atcaggcggt
    15301 cgccatcgcc gtccaaggca ataccgtaat cggcttcgtt ttgcaacacg gcggcttgga
    15361 gggttttggt atgggtcgcg ccgcattttt cgttgatgtt gtagccgttg ggttcgttgc
    15421 cgatgctgac gacctgtgcg ccgagttcgt ggaacacttt gggtgcaacg ccgtatcctg
    15481 caccgttggc ggtatcgata accaacttca aaccccgaag gtcggaatgg ccggggaagg
    15541 tggatttgca aaactcgata tagcggtcgt ccgcaccgct gatgcggcgg gcgcgtccga
    15601 gacgtgcgga cggttgggtt ttcatttctt catcgatttt ggcttcgatt tccaactcga
    15661 cttcatcgga aagtttcacg ccgccttcgg caaagaattt gatgccgttg tcggaatagg
    15721 tgttgtgcga cgcggaaatc atcacgccgg cggacaggcg caatgcgcgg gtcagataag
    15781 ccacaccggg cgtgggcaac ggcccggtct gtaccacatt cacacccgcc gccgtaaagc
    15841 ccgctaccag cgcggcttcc agcatatagc ctgaaatgcg cgtgtctttg ccgatgagga
    15901 cggtcggttt ctgatcggta tcgtgctgca ccagcacctg ccccgccgca tagccgagtt
    15961 tcaatacaaa atcgggcgta atcggaaact gaccgacttc gccgcgcacg ccgtccgtgc
    16021 cgaaatattt ttttgccatc atttgctccg tgaatgtgaa ccgttgtccg agattataca
    16081 gtcagtttgc gccctgctgc ctgcaccgtt gatgccgtct gaagccgtcc cgcccttttc
    16141 agacggcatt cggcgcaaaa accgccgttt accggttcac gcccaacgct tcccacacct
    16201 tcaacgcgtc cgccgtcgcc ttcacatcgt gcacgcgcac gatttgcgct ccgcgtgcca
    16261 cggaagccaa agccgctgcc acgctgccgt gtacgcgcgc cgccgcgtcc gcctcaccgg
    16321 tcagcttacc tatcatgctt ttgcgcgaca caccgatcag cagcggcaaa ccggtttccg
    16381 ccatcaattc gggcaaatgc cgcatcagtg cgatattgtg ttgcaggttc ttgccgaagc
    16441 cgaaacccgg gtcgagcgta atccgttgcg gcgcgatgcc tgccgcgaca caggtttctg
    16501 accgtgtttt cagataacgt gccacttcgc cgaccacatc ctgatatttc ggattgtcct
    16561 gcatggtttc gggcaagccc cgcatatgca tcaggcaaat gcccgtgtcc gcctggcgcg
    16621 ccagcaattc gaccgcacct tcgtcggtca acgccgccac atcattgata atatcgatgc
    16681 cgccgagtgc caacgccttt tccataacca ccgtgcggcg cgtgtccaaa ctgacgggaa
    16741 cgccccaccc cgccgcttcc gccaatacag gctcaacccg cgcccattct tcttcgggcg
    16801 gaacgaaatc cgcacccgac cgcgtcgatt cgccgccgat gtcgagaatg tccgcaccct
    16861 ctttcaaaag ccgttcggca tgcgccaaag ctgtttgggc gttttgcgaa tacgcgccgc
    16921 cgtcggaaaa agaatcgggc gtgagattca cgatgcccat gattttcggt ttgtccaaac
    16981 cgatttcaaa ccgtcctgcc cgccaaacgt gtcgtgccat ctgaactcct cccgaaataa
    17041 aaaacagatt atatgccgtc tgaaaccgtc ttgtgcgctt cggacggcac cgctattcgg
    17101 gcggcagacg gcatgttgtc ccaatgtctg cgccgccttt gaatctgccg gcatgcctgc
    17161 tatccgcccg ccttttcaaa acaggttccg acgattccgc acgcgcctgc cgcctttgcc
    17221 aagccgtaca ggatttcctg cggcatatcg cggctccata atcccgtaat attcgcaatc
    17281 acgggcagat ggctgatttg gcggactttc acgatggatt cgacatccaa acggtaggga
    17341 tggcctttgg tatggcccaa cacgcccgac tcgcccagaa tcaggtggcg gttgccgcgc
    17401 gagacgacat attctgcggc attcaaccaa tcttcggcag aatggtgctt gtctttacac
    17461 agaaccaagg gaatgttcag gcttccggct tcattcaata cggcaagatc ggacatcagc
    17521 ccgccgccca aatacaggat gtccgctccc gcattcaaag ccgcttcgac atggcggacg
    17581 ttgcgaacgc gcaccaatac gggtttccct gcgccatgcg ccgatgcggc ctgttccgcc
    17641 aaccgtctgc accgtccccg cccttcatcc gcacttaaag tgtcgtataa gtttgccgaa
    17701 gtgaaaaacg gatccagaaa cactgcatcc gcattgctcc atactgacgg ttctgcagcg
    17761 atacggacgg tttccccgcc gccgaaagcc acgcctttgg cggcaacgcg gctgtcttcc
    17821 gcccgatttt cccgactgac ggttttccat gtgtccaaaa tgcggacggc tttctcgacc
    17881 tccggcagcg tctgtacctc cctgacgctc aaaaccctat cgtcgccgat tgcgccgatg
    17941 acggtacgct cgtcgccgtg agaaatgtgt tctcgcagac ctctgctgcg gatgaaggcg
    18001 acaacgccgg caatgtccgc ttcggcggca cgcctgctca tgacaataat catatttcct
    18061 cctgacacaa gagacggccc gcccaaaata ggatttttgc aagccgtgtt atactgtggc
    18121 gtgttttaca gattgttcgg gctatggatt tattatcggt tttccacaaa taccgtctga
    18181 aatatgcggt ggcggtgctg acgatgctgc ttttggcggc agtcgggctg cacgcttccg
    18241 tatatcgcac cttcacgccc gaaaacatcc gcagccgcct ccaacaaagc attgcccata
    18301 cccaccggaa aatctcgttt gatgcggata tacggcgcag gcttctgccc cgcccgaccg
    18361 tcatcctgaa aaacctgacc attaccgaac ccgacggcgg ccgggtcgcc gtttccgtca
    18421 aagaaaccaa aatcggattg agctggaaaa acctgtggtc ggatcggata caggttgaaa
    18481 aatgggtggt ttcgggtgcg gatcttgccc tgacgcgcga cagaaacggc gcttggaaca
    18541 tccaagacct gttcgacggc gcgaaacact ccgcctcagt caaccgcatt atcgtcgaaa
    18601 acagcaccgt ccgcctcaat ttcctgcagc aacagcttat cctgaaggaa atcagcctca
    18661 acctgcaatc ccccgattcg tcggggcagc agtttgaaag ttcgggcata ctggtttgga
    18721 gaaagctgtc cgtcccgtgg aaaagcaggg ggctgttcct ttcagacggc atcggcacgc
    18781 ccgaaatctc accgttccat tttgaagctt ccacttcgct ggacggacac ggcatcacca
    18841 tttccaccac cggcagccct tctgtccgct tcaacgccgg cggagcggat gccgccggcc
    18901 tcggcctgcg tgcagacact tccttccgca acctccacct gaccgcgcaa atccccgcac
    18961 tggcactcaa aaacaacagc atcaaaaccg gcacggtcaa cggcacgttt accgccggcg
    19021 gcgaatatgc ccgatgggac ggttcgttca aactcgacaa agccaacctg cactccggca
    19081 tcgccaacat cggcaacgcc gaaatctccg gcagcttcaa aacaccgcgc cttcaaacca
    19141 atttctccct cggctcgccg ttggtttgga gtcgggacaa cgggctggac gccccgcgcc
    19201 tgcacatatc gacccttcag gataccgtcg accgcctgcc gcaaccccgt ttcatcagcc
    19261 ggctcgacgg ttcgctgtcc ataccgaatc tgcaaaattg gaatgccgaa ttaaacggca
    19321 cattcgaccg ccaacccgtt gccgcaaaat tcaaatatac gcgggaaggc gcaccgcacc
    19381 tggaagccgc cgccgcgctg caaaaattaa acctcgcccc ctatcttgac gaatttcggc
    19441 aacaaaacgg caaaatattc cccgacatcc tcggcaggct gtccggcaac gtcgaggcac
    19501 acctcaaaat cggcagcatc caactccccg gcttgcaact ggacgatatg gaaacctacc
    19561 tccacgccga caaagaccat atcgcgctca gccgtttcaa gtcagggctt tacggcggcc
    19621 ataccgaagg cggcatcagc atcgccaaca cccgtcccgc cacttaccgc ctgcaacaga
    19681 atgcaagcaa catccaaatc caaccgctgc tgcaagacct gttcggcttc cacagcttca
    19741 gcggcaacgg cgatgcggtc atcgacctga ccgcaagcgg cgaaaaccgc aaacagctta
    19801 tccgctcgct gcaaggcagc ctgtcgctga atatttccaa cggcgcgtgg cacggcatcg
    19861 atatggacag cattttaaaa aacggccttt ccggaaaaat ctcgggcagc acacccttct
    19921 accgattcac gctcaacagc gaaatttcag acggcatcag ccgccacatc gataccgaac
    19981 tcttctccga cagcctctat gttaccagca acggctatac caatctggat acgcaggaat
    20041 tgtctgaaga tgtccttatc cgcaacgccg tccatccgaa aaacaaaccg attcccctga
    20101 aaatcaccgg tacggtggac aagccgtcca ttaccgtcga ttacggcagg ctgaccggcg
    20161 gcatcaattc gcgcaaagag aaacagaaaa tcctcgaaga caccctgctg gaacaatggc
    20221 agtggctcaa acctaaagaa ccgtaaatac cctgcgtaca aaaatgccgt ctgaaacaca
    20281 ttaatcttca gacggcatga ccgtaaaacc tacaacccca atttccccca aatcccatca
    20341 attttaaccg taaccgcagg gtcttttttg attacccgtc cccattcgcg gtcggtttct
    20401 cccggccatt tgctggtcgc atccaaaccc attttgccgc cgagtccgct gacggggctg
    20461 gcgaagtcga ggtagtcgat gggcgtgttt tccaccaaaa cggtgtcgcg gacggggtcc
    20521 atgcgcgtgg ttaccgccca gatgacttct ttccagtcgc gcacgtttac atcgtcatcc
    20581 accacgatga tgaatttggt gtacataaac tggcgcagga acgaccagca gcccgtcatc
    20641 acgcgcttgg cgtgtccggc gtactgtttt ttcatgctga ccaccgccat gcggtaggaa
    20701 cagccttcgg gcggcaggta gaaatcggtg atttcggaga actgcttttg caaaagcggt
    20761 acgaacactt cgttcaacgc cacgcccaaa acggcaggtt cgtcgggcgg tttgcctgtg
    20821 taggtagagt ggtaaatcgg gttttcgcgc atggtgatgc gttcgaccgt aaacacaggg
    20881 aaatggccct gctcgttgta atagcccgtg tggtcgccgt atgggccttc caacgcggtt
    20941 tcgtttggat gaatcacgcc ttccaataca atttcagcac gggcaggcac ttgcaaatcg
    21001 ctgccgatac atttcaccag ctccgtccgc gaaccgcgca gcagtccggc aaactggtat
    21061 tcgctcaaag tatcgggaac gggcgttacc gcgcccaaaa tggtggaggg gtcgcaaccg
    21121 agtacgacgg cgacgggata cggcgtatcg ggattgagtt tgcggaattc ctgataatcc
    21181 aacgcgccgc cgcgatgcga cagccagcgc ataaccagct tgtttttgcc gatgagttgt
    21241 tggcggtaaa tgccgagatt ttggcgtttt ttgtgcggcc cgcgcgtgac ggtcaagccc
    21301 cacgttacca gcggcgcaac gtcttccggc cagcaatgtt gaatcggaag ctgatacaaa
    21361 tcaacgtctt ctccttccca cacgatttcc tgacacggcg cgtttttcac cacgttcggc
    21421 gccatgctcc aaatatcttt caacagcggc agtttggaaa acgcgtcttt gatgcctttg
    21481 ggcggttcgg gttctttcaa atacgccagc gtctgcccga tttcgcgcag cttggacacg
    21541 ctgtccgcgc ccatgcccat cgccacacgt tcgggggtgc cgaacaggtt cgccaacacg
    21601 ggataatcat agcgcgtacc gtcgggctta atcgggtttt caaacaacaa cgccggccct
    21661 tcggcgcgca acacgcggtc ggcaatttcg gtcatttcca aatgcgggga aacggggtgg
    21721 gcgacgcgct tgagcttgcc ctcctgctcg agcatagcga tgaagtcgcg caggtctttg
    21781 tatttcatgt ttatcctttt tgtcctttta tcctgaacaa tccgattcgg ataccgcccc
    21841 tatccttgcc tgcgcttcgg catatcccat gccgtgataa aagtcgcgta ccggcggatg
    21901 tccgccgctt tgatgaagtt gcagcaaagg gcgttggttg tcggactggg taacgacatt
    21961 gcagtgcaga ccgaaggtgt cggtttcata agggggcagc cgattgcaaa tcataccgaa
    22021 ataaacggcg ttttcagggt tgtcgtaaaa gcggctttga tagtcgttaa aactcttttc
    22081 gctgacggac acccacacgc cgtattccag cgtttcctga tgcccgataa tcggaatagg
    22141 cagcgccgcg cggataaagc ggtcggtttg gccggaatag cggatgatgc agaaatcaga
    22201 atcgcattcc gcttgataag caatgcgttc ttcttcactg agttgattat agggataggg
    22261 ggcggtaaag ccggttgcgg gcatatcttc atgtttttcg ccgcaggaag tgcaagtgtg
    22321 cataaggttt cagactttca acacaagttt gcggtaaagc cattcgccgg caaacagcat
    22381 ccccatcaat acataggcaa tcacgcctgt ataaaccgcc caccaatcat atcgccccaa
    22441 ccgggccaac aaagcggcaa gcgtcccgtt ggcaataaag aatacgcacc aaacctgcgt
    22501 tacccggcgg gtatagcgca cggctttttc aggcaggtcg ggctgttgca gccgcgcaag
    22561 tttttcaatc accgtctgcc cggcaaacag gctgctgccg aacaccgcca acatcatcaa
    22621 attaacgagg acaggatacc aatacattga atcacgccgc ccgaacacca atactgcggc
    22681 aaaaaacagt gtaatatagt ggattaaatt taaatcagga caaggcggcg aagccgcaga
    22741 cagtacaaat agtacggcaa ggcgaggcaa cgccgtaccg gtttaaattt aatccactat
    22801 accacaaagc gggataggca atgcttaata cggtcaaaaa aatatgtccg aaaaaaccgg
    22861 gtttcatttt gaatccgcac aaacgttttc agacggcatc cgataaaaac atgccgtctg
    22921 aaaaataatt agaaataccc gattagccgg cctgaatctt caataccgcc tgtaccacgt
    22981 cgttgacggt gcggacattg cggaaatctt cagcctgcag cttgcgggct gccacaacca
    23041 ttcacgattg cgatataccc ggcggcattc ccgactgccg ttcagcagaa aacgcaccca
    23101 ttccaaaccg ctccaatatg cctcgggcag cataccggct tcagacggca tatcgtccga
    23161 agcagacaaa gtcaggctgt aacgcgtccc tttggtcaga accatcgcca aagcataagc
    23221 aaacggcgcg cgcgttgccg atacggcata tccttccggc agcggatcgt ccgccgccaa
    23281 aaccaaaacc gacctgcatc cctcttccaa cagggacgcc gcttccgcca atgccgtttc
    23341 cacaccgtcg gcacacacgg ccaatgccgt ctgctcgctc atatcccgcc tcaatatcga
    23401 ccattgcccc gccgtcgcat tgtgcaccga caaaccgaac gaagtcggcg acacggtatg
    23461 cgatttcaac agttccaacc acaaatcgaa actacgcgcc atttccccgt cgtgcgaggc
    23521 ataaactacc ggactgccgg gatgggcgga ggcaatgtcc caagccgcgt cgcataccaa
    23581 acgcgccgcc ttactcaaac ggcggcgccg catagcgggc aggaacggca attcccgcct
    23641 gacatcgggc aaaccgtcgg caaaatccgg acattccgcc cactgcctct actgggccat
    23701 atcgcgcatt ttgccgcccg aaacccgcca gtcggcgatg tcgaagtgga accggcaaat
    23761 aatcggcggc atagtttctt tcaaaaattt acactgtgcc gcattctaac caaagcccat
    23821 ccccctgaca atgccgaaat tcaaacgcat ccacgccatt ttttccgaca acgccgcccc
    23881 tcggcaaacc gcccgaatta gcctgaattt acatttatca ttataatgcc cgattaccga
    23941 caccgcctcc ctgctgccgc acagcgggcg tatggttctg atagaccgca ttacccgata
    24001 cggcgatgat tttgtcgaag caggggcgca ggtaaaaccc gaccacatcc tgctggccgg
    24061 cggcatgctg ccctatacgg catttatcga actgatggcg taggctgtcg gcgcatatgc
    24121 cggcctccaa gcccgaaaaa aggcgcaacc ggtccggctc ggttttcctg cccggcacgc
    24181 gcaaacttga aatttttacc caatccgtcc ctgtcggaac acatttgccg gcaacggcgc
    24241 atatgtctat ccaagatgcc ggggtatggg cgtgtccgac tgcgaactgc gttggacgga
    24301 cgcgccggaa accttgtctg aaacactcct ttcagacggc attttggcgc gcgcctcact
    24361 caacgtgtac agccccgaac accctgtcga aacaacttaa tgccgtctga acaggcacgc
    24421 acatacggag aacaaccatg actgaaaccg tcctgattac cggctcaaac agaggcatag
    24481 gcaaagccgc cgcactcggt tttggcggac gacggctttg atatcgccgt ccactgccgc
    24541 agccgccgcg acgaagccga agccgtggcg gaagaaatcc gcgctttggg cagaaatgcg
    24601 cgcgtgttgc agtttgacgt gtccgaccgc gaagcctgcc gcgagattct gaccgccgac
    24661 atcgaagcaa acggcgcgta ttacggcgtg gtgttgaacg ccggcctgac gcgcgacaat
    24721 gccttccccg cgttttcaga cgacgattgg gatgtggtgc cgcggactaa tttggacggt
    24781 ttttacaatg tattgcatcc gctggttatg ccgatgatac gccgccgcaa agccggacgg
    24841 attgtgtgta tggcatcggt gtccggcctg acgggcaacc ggggacaggt caattacagc
    24901 gcgtcaaaag cgggcattat cggcgcggca aaagccttgg cggtcgaact ggcgaaacgc
    24961 aaaatcaccg tcaactgcgt ggcgccgggt ttgatcgata cggagattgt cgatgaaaac
    25021 gtacccgtcg gagaaatttt gaaagccgtc cccgccgcgc gtatgggact gccggaagaa
    25081 gtggcgcacg cagtgcactt cctgatggat gaaaaagcgg cgtatatcac gcgccaggtg
    25141 attgcggtga acggaggttt gtgttgaata ccagaagggt cgcagtaacg ggcataggcg
    25201 gcattaccgc cttcggccgg gattggcaaa gcatacaggc agcattcaaa gccgaaaaaa
    25261 acgccgtcaa atatatggat tggcgcgaac gtttccccga attggaagcg caactgggtg
    25321 cgccgattga ggattacgcg ccgccgaaac attggacgcg caagcggctc agaagtatgg
    25381 ggcgcgtgtc gtacctgtgc gtcgatgcgg cggagcaggc tttgatggat gcaggtttgc
    25441 tcggggacga aagcattacc gacggacgga tgggcgttgc ctgcggctct tccggcggcg
    25501 gcaccaaaga catcggcgat gtgggcgaat tgttgctgac cggcacgtcg cgcaacttcg
    25561 gcgccaatac ctatgtgcgt atgatgccgc acaccgccga cgccaatatc ggcatctttt
    25621 tcggattaat aaggacgcat catcccgaca tcgagtgcgt gttcttcggg cagccaaggc
    25681 ataggttatg cttacgaggc catcaaatac ggtctgaccg atataatgct ggcgggcggc
    25741 ggcgaagaat tttgcccgtc cgaagtgtat gttttcgact cgctctatgc cgccagccgc
    25801 cgcaacggcg aaccggaaga gaccccgcgt ccatacgacg cgaaccgcga cgggctggtc
    25861 atcggagaaa aggcgcgggg attttcgtat tggaagaatt ggaacacgcc aaacggcgcg
    25921 gtgcgaaaat ttacgccgaa ctcgtcggct acggcgccaa cagcgacggc atcaatatcg
    25981 gcgaaggcgc agccgtattc atcatgacgc gcgatgcgga tttttccggc ggtatgcagc
    26041 ttctgggtta cggcgcaagc agcgatgcct accatatttc cacgccccgc cccgacgcaa
    26101 aggcgcaatc ctcgcctttc agacggcatt gcagcacgcc ggccttgcgc ccgaagacat
    26161 cggccggatt aatctgcacg gcaccgggac gcaccacaac gacagtatgg aaagccgcgc
    26221 cgttgcagcg gttttcggca acaatacgcc ctgcacgtcc gccaagccgc aaaccggaca
    26281 cacgctgggc gcggcgggcg caatcgaagc cgcgttcgcg tggggcattg ccgaccggca
    26341 aagcaatccc gaagggaaac ttccgccccg gctttgggac gggcagaacg accccgacct
    26401 gcccgccatt aacctgaccg gcgacggctg ggaaaccgaa aaacgcattg ccgccagctc
    26461 gtcgtttgcc ttcgggggaa gcaactgcgt cttaatcatc ggatgaaata agtttgtcaa
    26521 tcccaccgct atgctataca atacgcgcct actcttgacg ggtctgtagc tcaggggtta
    26581 gagcagggga ctcataatcc cttggtcgtg ggttcgaacc ccaccggacc caccaattcc
    26641 caagcccgga cgtatgtttg ggcttttttg ccgccctgtg aaaccaaaat gctttgagaa
    26701 accttgataa tgaaaaaagt cagcgtattg attgttgcca aaaacgaagc aaaccacatt
    26761 cgggaatgta ttgaaagttg ccgtttcgat aaagaagtta tcgttatcga cgaccatagc
    26821 gccgacaaca ctgccgaaat tgccgagggt ttgggtgcaa aagtcttcag acggcatttg
    26881 aacggggatt tcggagcgca aaaaacattt gccatcgaac aggcaggcgg agaatgggtt
    26941 ttcctgattg atgcagacga acgctgcacg ccggaactat ctgatgaaat ctcaaaaatt
    27001 gtccgaaccg gcgattatgc cgcctatttt gtcgaacgcc gcaacctttt ccccaaccat
    27061 cccgccacac acggcgcgat gcgtcccgac agcgtatgcc gtctgatgcc gaaaaaaggc
    27121 ggttcggtgc aaggcaaagt acacgaaacc gtacagaccc cctaccccga acgccgtctg
    27181 aaacatttta tgtaccatta cacgtacgac aactgggaac aatatttcaa caagttcaac
    27241 aaatatactt ccatctcggc cgagaaatac cgggagcagg gaaagcccgt gagtttcgtt
    27301 agggacatta tcctccgtcc gatttggggt tttttcaaaa tttatatcct gaacaaaggg
    27361 tttcttgatg gaaaaatggg ttggattatg tccgtcaacc acagctatta cacgatgatt
    27421 aaatatgtca aactatatta tctgtacaaa tccggcggaa aattttaaat ggaaaaagaa
    27481 ttcaggatat taaatatcgt atcggccaag atttggggtg ggggcgaaca atatgtctat
    27541 gatgtttcaa aagcattggg acttcggggc tgcaccatgt ttaccgccgt caataaaaat
    27601 gatgaattga tgcgcagacg cttttccgaa gtttcttccg ttttcacaac gcgccttcac
    27661 acgttcaacg ggctgttttc gctctacgcg cttacccgct ttatccggga aaaccacatt
    27721 tcccacctga tgatacacac cggcaaaatt gccgccttat ccgtactttt gaaaaaactg
    27781 accggggtgc gcctgatatt tgtcaaacat aatgtcgtcg ccaacaaaac cgatttttat
    27841 caccgcctga tacagaaaaa caccgaccgc tttatttgcg tttcccgtct ggtttacgat
    27901 gtgcaaaccg ccgacaatcc ctttaaagaa aaataccgga ttgttcataa cggtatcgat
    27961 accggccggt tccccccgtc tcaagaaaaa cccgacagcc gtttttttac cgtcgcctac
    28021 gccggcagga tcagtccgga aaaaggattg gaaaacctga ttgaagcctg tgtgatactg
    28081 catcggaaat atcctcaaat caggctcaaa ttggcagggc acggacatcc ggattatatg
    28141 tgccgcctga agcggggcgt atctgcttcg ggagcagaac catttgtttc ttttgaaggg
    28201 tttaccgaaa aacttgcttc gttttaccgc caaagcgatg tcgtggtttt gcccagcctc
    28261 gtcccggagg cattcggttt gtcattatgc gaggcgatgt actgccgaac ggcggtgatt
    28321 tccaatactt tgggtgcgca aaaggaaatt atcgaacatc atcaatcggg gattctgctg
    28381 gacaggctta cgcctgaatc tttggcggac gaaatcgaac gcctcgtctt aaaccccgaa
    28441 gcaaaaaacg cactggcaac ggcaggacat caatgcgtcg ccgcccgttt caccatcaac
    28501 cataccgccg acaaattatt ggatgcaata taagccgctt tcagacggcc tatgccgtct
    28561 gaaagccttt gatgcaacaa accgccaaat tatattcgtt cattggaaaa aaacaccccg
    28621 aattcatcct tcaaaataag aaaatcccaa tatcccccga tatcgcgcag cctattggca
    28681 aagttttgca gcgtcttccc cggcttgtgc cgccgcgtca agtgctttgt tacaatgcac
    28741 taccttcaca tttcttaata aattttatga gtaaccatac ttcttggtcg tccaaaatcg
    28801 gtttcgtact tgctgcggca ggttcggcca tcggtttggg cgcgatttgg aaatttcctt
    28861 atacggcggg caccaacggc ggcgcggtgt ttttcctgct gtttttgata tttaccgtct
    28921 tggtcgccct gcccgtccag cttgccgaat tttatatcgg gcgcacgggc ggtaaaaatg
    28981 ccgtcgattc cttcagggtt ctgcgtccgg gtacgcaatg gctttgggtc gggcgcatgg
    29041 gcgttgccgc ctgctttatt ttgctgtcgt tttacagcgt ggtcggcgga tgggtattga
    29101 attatgtcgt ccacagtttt acaggggcaa tccatgcggg agcggacttt gaagccctgt
    29161 tcggcacaac gatttccaat ccggcaggtt cgctgtccta tcaggcactg tttatgctga
    29221 ttacggtttg ggtggtcaaa ggcggcattt cagacggcat tgaaaaggca aaccgttatc
    29281 tgatgccggg gctgtttatc ctctttattg cgctggctgt ccgttcgctg acgctgccgg
    29341 atgcaatgga gggcgtgtct ttcctgctca aacccaattg gtcgtacttt aaagccgata
    29401 cgatgattac ggctttgggc caggcgtttt ttgccctgag catcggcgtt tccgccatga
    29461 ttacctatgc ttcgtatttg ggaaaagatc aggatatgtt ccgttccggc catacgatta
    29521 tgtggatgaa cctcttggtt tccctgcttg ccggcctggt gattttcccg gcggtattcg
    29581 ccttcggttt tgaaccgaac caggggccgg gattgatttt tatcgtattg cccgcagtgt
    29641 tcatgaaaat gccgttcggt acggttttat ttgcagtatt catgcttctg gtcgttttcg
    29701 ccacgctgac ttcggcattt tcgatgttgg aaacggtcat tgcctcaacc atccgccaag
    29761 acgagcgcaa acgcaaaaaa catacttggc ttatcggcac ggctattttc attgtcggca
    29821 tcccgtccgc gctgtctttc ggcgcatggg gtgagtttaa ggttttcggc aaaaccattt
    29881 ttgatttgtg ggactatgtt atttccgccg tcattatgcc gattggtgct ttgagtgttt
    29941 ccatctttac cgcctggatt caggacaagc agtctgtgtt aaaagatgcc ggcgcgggca
    30001 gcaccgtacc acgggcagtg ctgctgctgt ggctgaatac cttgcgctac cttgccccga
    30061 ttgccattat tattgttttc gtcaattctt taggcatcct ttaaacatac cgcccgacgg
    30121 caaaatgccg tctgaaagcc tttcagacgg cattttgctt cagattcaac ctatttcgtt
    30181 caaagtatag tggattaaat ttaaatcagg acaaggcgac gaagtcgcag acagtacaga
    30241 tagtacggca aggcgaggca acgccgtacc ggtttttgtt aatccactat attgtccggc
    30301 atggccagcc taactttaag ccgcctgctt tcggcaaccg accgtatcag cggcaaatcc
    30361 atcacggaaa acgccgtcga caaggcatcg gcaaccgccg catcatccgc cattacgctc
    30421 atgcttttat aacgcggcgt actcacgccg gttcgcggat cgaacaaatg ggtaagcctg
    30481 cccgctttgt ccatcaccgt cccatagccg cccgaagtgg caaacgcctt gtctttcatc
    30541 gtgatatttg ccaacacgcc ttcttcgtcg tccggattgc ggatgccgac attccacatc
    30601 cgcctgccgt ttgtatcaaa ccccctgatt tcgcccatat cgaccagcgc ggcaggcact
    30661 ccgtttgctt taagcagcgc aacaaccttg tccgtgatat agccttgcgc gatgccgttc
    30721 aaggacaaac ccataccctt ttcggcaaaa cggatttcac ggtcgtcaaa cgagactttg
    30781 ccgaagccga cccgtttcag ggtttccttc acgctattct ccggcggcgt ttccgcatcg
    30841 ggacggccgg caaaataatc ggcatacagt ttccacaacg cctgaaccgt cggatcgaac
    30901 gcgccttccg tcagcgcgtg aatatcgcgg ccaggctcaa cagttccaaa aaatccgccg
    30961 gaggcgaagt cagataaccg tcgcggttca gacggctgat caggctgtct tcacggtaaa
    31021 ggctgaacat tttttccaaa cgcgccactt ccgccaaaac cttattgacc aaatccgccg
    31081 cctgtctgtc gtccacgccg aacaggcgca gctccgcgcc ggaacccagc gcgacacctt
    31141 tccagaaaaa cacattttca tcgcgttttt tatccatgtt gcgtttttcg ccgccggcgg
    31201 caaaaggatt cggcaaaaaa gaaaccgccg caccggccac agcaacggcg gcagccgtca
    31261 gaaaacgtct gcgcccgaaa tgcctgccca taccgcctcc taaaccgaca ctaccgcctt
    31321 gatgtgcgga tgcggatcgt agccttccaa ctcgaaatct tcaaacttga aggcaaacaa
    31381 atccttgact tcaggattga ttttcatcac aggcaaggcg cgcggttcgc gttccaactg
    31441 caatgcggcc tgttcgaaat ggttacggta caaatgcgcg tcgccaaacg tatggacaaa
    31501 ctcgcccgcc tccagcccgc acacttgcgc catcatcatc gtcaacagcg cgtagctggc
    31561 aatattaaac ggcacaccaa ggaaaatatc tgcactacgc tggtaaagct ggcaggacag
    31621 tttgccgtcg gcaacgtaaa actgaaacag cgcgtggcag ggcggcaagg ccatctcatc
    31681 gaccaaagcc ggattccacg ccgacacaat caggcggcgc gagtcggggt ttttcttaat
    31741 ttgttccacc acattggcga tttggtcgat atgcctgccg tcgggcgcgg gccagctgcg
    31801 ccattggtag ccgtaaaccg gacccaagtc gccgttttcg tccgcccact cgtcccaaat
    31861 ggaaacattg ttatccttca ggtatttgat attggtatcg cctttgagga accaaagcag
    31921 ctcgtggatt atcgagcgca aatgcagctt tttggtcgtc agcagcggaa aacctttgcc
    31981 caagtcaaaa cgcatctgat aaccgaatac ggagcgcgta cccgtaccgg tgcggtctga
    32041 tttgtccgta ccgttgtcga ggacgtggcg catcaagtcc aaataggcct tcatcatcat
    32101 ctttcatcaa attaaacggc acatattgta acatttccgg ccaacacccg agtccggaca
    32161 aacgcgcagg ccgccccgcc ggacgctcaa aacgcggatg aaggcaggca ggattgttgg
    32221 caatttcagc cctttttcac agcaaaagca ggcgtgaaaa caaaattatc ttgattggaa
    32281 tcaaaaaatc tagtttaact acttagaata aaattccaat aatatttttt atttgagaaa
    32341 ttaatttatg attattggtt gattatcggc aataaacatc atatatcgaa aatatgggaa
    32401 aaataatgtc aacaatttgt gcaaaattag gcttggcatc agaaaaaaat aggtttatat
    32461 tgccaactac aaatttgttt tcccattagt acaatatcaa ccaaaaggag tatccgaatg
    32521 actgacctga acaccctgtt tgccaacctc aaacaacgca accccaatca ggagccgttc
    32581 catcaggcgg ttgaagaagt cttcatgagc cttgatccgt ttttggcaaa aaatccgaaa
    32641 tacacccagc aaagcctgct ggaacgcatc gtcgaacccg aacgcgtcgt gatgttccgc
    32701 gtaacctggc aggacgacaa aggacaagtc caagtcaacc gcggctaccg cgtgcaaatg
    32761 agttccgcca tcggccctta caaaggcggc ctgcgcttcc acccgaccgt cgatttgggc
    32821 gtgttgaaat tcctcgcttt tgaacaagtg ttcaaaaacg ccttgaccac cctgcctatg
    32881 ggcggcggca aaggcggttc cgacttcgac cccaaaggca aatccgatgc cgaagtaatg
    32941 cgcttctgcc aagcctttat gaccgaactc taccgccaca tcggcgcgga caccgatgtt
    33001 ccggccggcg acatcggcgt aggcggacgc gaaatcggct acctgttcgg acaatacaaa
    33061 aaaatccgca atgaattcac ttccgtcctg accggcaaag gtttggaatg gggtggcagc
    33121 ctcatccgcc ccgaggcgac cggctacggc tgcgtctatt tcgcccaagc gatgctgcaa
    33181 acccgcaacg atagttttga aggcaaacgc gttctgattt ccggttccgg caacgtggcg
    33241 caatacgccg ctgaaaaagc catccaactg ggtgcgaaag tgctgaccgt ctccgactcc
    33301 gacggcttcg tcctcttccc cgacatcggt atgaccgaag cgcaactcgc cgccttgatc
    33361 gaattgaaag aagtccgccg cgaacgcgtt gccacctacg ccaaagagca aggtttgcaa
    33421 tactttgaaa accaaaaacc gtggggcgtt gccgccgaaa tcgccctgcc ctgcgcgacc
    33481 cagaacgaat tggacgaaga agccgccaaa accctgttgg caaacggctg ccacgtcgtt
    33541 gccgaaggcg cgaatatgcc gtcgactttg ggcgcggtcg agcaatttat caaagccggc
    33601 atcctctacg ctccgggcaa agcctccaac gccggcggcg tggcaacttc gggcttggaa
    33661 atgagccaaa acgccatccg cctgtcttgg gcgcgcgaag aagtggacag ccgactgttc
    33721 ggcatcatgc aaagcatcca cgagtcctgc ctgaaatacg gcaaagtcgg cgacaaagtg
    33781 aactacgtca acggtgcgaa catcgccggc ttcgtcaaag ttgccgatgc gatgctggcg
    33841 caaggcttct aagcaaacgc ctccgccccg caaacaaaat gccgtccgaa ccgcaaatgc
    33901 tgttcagacg gcatttcatt atccgcccgt tcgaatcggg taatactata gtgaattaaa
    33961 tttaaatcag gacaaggcgg cgagccgcag acagtacaaa tagtacggca aggcgaggca
    34021 acgccgtact ggtttaaatt tagttcacta taccgataca tccgaatatg ccagaccgtc
    34081 tgaacggtat tcatacctcc caatcctgca cgcgcttcaa atcgttttgc gccaaagtat
    34141 ccgcgtggcg gttacggctc tgatattccc tgtctttcaa tatgctgctc gccacataat
    34201 tcaaatgagc ctttgccgcc tccgaagcct cgcccggccg gcggttcgat atcgcctcat
    34261 acagcacgcg gtgctgcgcc atcagtttcg gacgcggatc ttcttcctga ttcagataaa
    34321 taaggctgct gcgcgtctgc cggtacagca ttttcaacaa accgcccgac aaatggctga
    34381 acaacaaatt gtgcgccgca tccgcaatcg tctgatggaa gctgacatcg gcttcgccct
    34441 gatgttccag attgccgctt ccgcacgctt cctcaaactt ttcaagccaa aaccgaatcc
    34501 gcttcaaatc ggcatccgtc cggcgttctg ccgccaatgc cgccatacag ccctcgatgt
    34561 ggcagctgaa atcaaaaaca tcctgttccc aattggaatg tttgcccaaa agctcctgcc
    34621 aactttgcaa aaaatcctgc tgcggcttga ccgaaacata ataaccatcg ccctgcctcg
    34681 cttccaaaat ctgacgggca accaaaatat tcaatgccga ccggaccgac ggacgcgaaa
    34741 cgccgaactc ttccgccaaa acgcgttcgg gcggaatctt gcccccttcc gcgtaaaccc
    34801 cttccacgat acggccttcc aataccgaca atacctgatc gctgattttc tgaggcctta
    34861 ccagtttcat cactcctcct ttataaagat tccctgcaga acccttccga aatacatcaa
    34921 acatcaaatt ggactggcca atcagggcgg attctaatga cacgccgttc ccgccgtcaa
    34981 cggcacttac ctcgcaccgc ccccgaaaca caggaaaaaa ctacaccaaa ctacaatttt
    35041 tgttcatgca aatatttgtt ttaacgggat ttaaatgaaa gctccgattc aaatctgccg
    35101 aaccgcccaa aaatatattg acctgaatat taaagtttcg taaagtaatg caacgttgct
    35161 ttaattggtt tgaccactat tgccgacgat tagaaaaata ttttcggaga tgttcaatta
    35221 tggaaacttg ggttcaaaac tacacggcaa tcggcggcag cctgtatctg actgccgccg
    35281 ccgcactctt acccatcgtc tttttctttg ccgcgctgac cgtcctgaag ctgaaaggct
    35341 atcaggcggg gctttatacg ctgctgattg cgcttgccgt tgccgtattc ggcttcggga
    35401 tgccgacggg tatggcggtt tcttccctgt cgccgcagcc ggattgaccc aacgccccat
    35461 acgctacact ggacttgacc gcgcattaca aaatcggtaa atccacccgc atcggtttgg
    35521 actttgaaaa cgtgttcaac aaacgctacc gcacgatgcc ggatattcat gtttacggca
    35581 cgccgcgcag cctgaccgca accgtcaaac ataccttcta aacagatacg gcctgccgtc
    35641 ttaaaaggca gaccggcacc ggaaaccgtt ttaaagaaag aaatgccgtc tgaaacctta
    35701 tcgtttcaga cggcatttga ttgccctggc ggttatttgc cggtttgggt atcccgtttc
    35761 aatccgccgc cgagcgcctt gtacaaatcg gcaaggtttt cggcacgggt cagttgtgcc
    35821 gacaaagccg caccctccgc cgaatagctg ctgcgttccg catcgagcaa gtcgagcgcg
    35881 ccggatacgc cgtgtttgta acgcaggccg accaagcgca acgcttcttt agaggcgcgg
    35941 ctttgtttgc ttaaagcggc ataggcttta tccagctgct cgcgcgccgc caatgcgttt
    36001 gccacgtctt gaaaggcgga ttgtacggcg gattcatagg caacgatttg tgcctgttgg
    36061 cgcagcttgg ctgcatcaag gttggctttg ttcgtacccc aggtaaaaat cggcagggca
    36121 atagacggcg caaacgacca aacgcccgtg ccgcttttga acagcccgcc caattcggca
    36181 gaacccgtac cgacgcttcc ggtcaggcgg atggatggga aaaaggcggc gcgtgccgca
    36241 ccgatattgg cgtttgcctg tttgagcgcg tgttcggcgg cgcgtatgtc gggacggtcg
    36301 agcaatactt cggaactcaa accggccggc agcttctcaa caaaaaactg cttgtccaac
    36361 ggcaaaccgg cgggcaggtc gtcgggtatc ggttggttaa tcagggttgc caaggcattg
    36421 cgcgcctgct cgcggctgcg cgcggcatgg gcataatcgg ctttggcgga ttcgatcagg
    36481 gcttcctgct gacgcagggc gacggcggaa attacgcctg ccttataccg caattcggac
    36541 agcttgtagg tttcctcgcg cgttttcaaa acacgctgcg ccaaagacat cgcttcttcg
    36601 gcataacgct cgttaaaata ggctttggca acggtggcaa tcaggctcaa atgtgccgca
    36661 tcgcggttgg cggtgctggc aaaatagcct tgcagtgccg cctcgctgct gctgcggacg
    36721 cgtccgaaca ggtcgagttc gtaagatgcc gcacccagtc cgacattgta gctgctgctg
    36781 acattgccgc cgctcaaact gccttggcgc gagccgttcg cattggcggc aagcgtgggc
    36841 agaaggttgt tgcgctcgat catgtattgt ttgcggtaga tttcgctgtt caatacggct
    36901 gtacgcaaac tggtattgcg ttcgagtgcg atgtcgatca gcttttgcag gcgcgggtcg
    36961 gcaaaatagt cgtgccaacc caaatcaacc gcgcggatgc cgctgtcggc ggtatcgttt
    37021 ttaaacgttt ccgcaacttc gactttgggc tgctcgtatt ggggaatcat ggtgcaggca
    37081 gacaatgcaa aagctgctgc aacagaagtc aaggtagttt tcaatatagt atccataaaa
    37141 aaagtcctga tgccgtctga aaacccgtgg gcgttcagac ggcatggttg cttaatgttg
    37201 gctgtcgtcc gaaccggtga tgcccgcttc ggcggcgtgt tttactgcca tttcgtgctc
    37261 gtgcgcggtt tctttgaaga atttgcgcac caccacatag aaaagcggaa caaggaacac
    37321 ggataaaagc gtgccgatga gcatccccca gaatacggtc gtgccgatgg cgcgctggct
    37381 ggcagaactt gcaccaccgg caatatacag gggaaccacg cccaaaataa aggcaaacga
    37441 agtcataata atcggacgga aacgcaggcg ggccgcttcc aaagcggctt cgaccgcgct
    37501 tttcccttgc gcttgaaggt ctttggcaaa ttcaataatc aaaatcgcgt ttttcgcact
    37561 caaacccatc acggtaacga aaccgacttg gaagtagatg tcgttggcaa acgagggaac
    37621 gctgcccaac aatccttcaa acaggttacg cccggttacg cccgcagccg cgcccgccaa
    37681 gcccaaaggc atcaccagca gaacggccag cggaatagac cagctttcat aaagtgctgc
    37741 aagcaccaag aagacggcaa cggcggcaag cgcgtaaagg gcaatggttt gcgaaccgcc
    37801 ttttgcctct tcgcgcgact gtccgcccca ctccaggctg taaccgctgc ccaattcgtc
    37861 aaccattttt tgaactgcct ccatggcctg accggtggaa acaccggttg cgggcgaacc
    37921 ggacagttcc atagcgggat aaccgttgaa acgaacgctc tgttctgtac ccatctgcca
    37981 agaaacggtg gcaatcgaag aaagcggaac ggcaatgccc gagctattcg gcacagtcag
    38041 attcaagatg tcggcaggct gcatacgggc atccccgtct gcctgcacca tcacgcgctg
    38101 cagacggcct tggttcggga agtcgctgac ataagacgaa ctcagcgcgc ttgccaatgc
    38161 ggtgcggatg tcggcaaacg aaacgccttg cgccgccgcc gcggcacggt tgatgtcgat
    38221 tttcaactgc ggcgcgtctt ccaaaccgcc ggcacggacg gtacttgggt taaacaaacc
    38281 gctggtacgc attttctgaa tcaactcgtt gcgtttggcc agcaatgcgg catgaccggt
    38341 attgttgcgg tcttgcaggt tgatgctcaa accggaaccg ttgcccaact ccataatcgg
    38401 aggcggaacg atggcgatgc cgaaaccgtc tttaagcgtc cccatcatca taccggtcaa
    38461 tttgcctgca accgattccg catcgctgcc cggggtttta cgctcgttcc agtctttgag
    38521 catggcaaaa cccattgcca tattctgacc gctgcccgaa aagctgaagc cggaaacggt
    38581 aatgatgttt tcaatttcag gaatactttt tgccaactgc gttacttgcg ccagcgtcgc
    38641 atcggtgcgc tctttggtcg cacccgcagg cagttgcacg ctgaccataa cgaagccttg
    38701 gtcttcggtc ggcaggaacg aagtcggcag gcgcataaac aggaatacgc ccacaaccgc
    38761 caaaccaacg taaacaacca tcatgcgcaa agtcttgcgc aatactttgg caacccagcc
    38821 ttcgtagccg tgtgtccagt tgtcgaattt tttgttaaac caaccgaaga aacctttttt
    38881 ctcttcgtga tgccctttcg ggatggtctt caacattgtg gcacacaaag caggtgtgag
    38941 tgtcagcgca aggaaggcag agaatgcgat tgatgccgcc atagtcagtg caaactgttt
    39001 atagatattg cccgttgccc cgctgaacat agccagagga acgaatacag acatcaaaac
    39061 agcagtaata ccgatgaccg caccggaaat ctgacccatt gcttttttgg ttgcagcctt
    39121 aggcggcagg ccttcacccg ccataatgcg ctcgacgttt tcaaccacca caatcgcgtc
    39181 atcgaccacg ataccgatga ccaaaaccat cgcaaacatc gttaatacgt taatcgacat
    39241 gcccatataa gagatgaagg cgaaaccgcc caacagggaa atcggtacga cgatggtcgg
    39301 aatcagcgta taacggatgt tttgcaggaa gaggtacatc acgacaaaaa ccagcaccat
    39361 cgcctctaac aaggtatgga tgactttttc aatcgaaatt tcgacgaatt tggaagtatc
    39421 gtaaggggtt ttccagctca taccctgagg aaagtatttt tccaacgtcg ccatgcgttc
    39481 tttaaccgcc tttgccgtcg ccatcgcatt accgctgttg gacagcatca ccgccatacc
    39541 ggtggtattt accccgttca gacgggttga ggaagaatag tcttccatac ccaatccgac
    39601 tttcgccaca tccttcaggt aaacattaga accatcagta ttggcgcgga ggatgacgtt
    39661 gccgaattct tctgccgtac tcaactgccc ttgcgccgtt acggtagccg taacagtctg
    39721 cccctgaaca gcgggaagcg aaccgatgga acctgcggaa atctggatat tctgcgccga
    39781 cagcgcgctg ccgatatcgg caaacgacaa attgtagttc tgcagtttct taggatcgac
    39841 ccaaatccgc atcgcgcgtt gcgcgccgaa caggcgtacc tgccccacgc cttcgatacg
    39901 ctgcaactcg ggaacgatat tacgctgcgc gtagtcgttc atctcctcgg ttgactgtac
    39961 gtctgacgac agcatcacaa tcatcaaaaa attggaacgc gctttggata cggtaacgcc
    40021 gtattgctgg acggtagcag gcaacatgct caatacttcg gaaagcttgt tttgcacctc
    40081 cacctgagcc aaatcttcgt cggtttcagg cgtaaaggtc aggctcacgc tgccgctgcc
    40141 gctcgaatcg gcagaagtgg acatataatc caaaccttcc acgccgttca tattccgctc
    40201 gataacggaa agtacgctgt cttccatcac ttgtgcggat gcacccggat aagtagccct
    40261 cagggtaata ttcggtgcgg caacagacgg atattgcgaa acaggcaggc ttttaatgcc
    40321 gaaaataccc gccgcaatga tgaaaatcga aataacccac gcgaaaatgg ggcggtcgat
    40381 aaaaaattta gccatcgatg ccttccttat tccgcttcag aagcaggttt ggcttcagat
    40441 gccgtctgaa cgccggattg aggcgcagca gcttggtttt cagacggcat ccattcttta
    40501 ggcgttacct ttttcgcacc cgtcatgccg gcgatactga tgccttccac aaccaccttg
    40561 tccccgtcct tcagacctga cgtaacaatc caattcgcac cctgctgttg tgcaatcgtt
    40621 acctcgcgtg gttccatacc gccttgggcg ttcacaatca tcacggtatc tttcgtaccg
    40681 cgcgttaccg cctgctgtgg aacgatgaat gcattatcca ccgccacttg atccatcagc
    40741 acgcgcacat acaggccggg catcaggata ttctgatcgt tcggtacggc ggcgcgcagg
    40801 gtaatctgac cggtcgattc gtcaacggtc gggtcggcaa acagcaggcg gcctttttca
    40861 ggataaaccg tgccgtcgtc aaatttgatg ccgaccgcaa tcacaccatc cgccgccagc
    40921 agcttgccct cggctatctg acggcgcagt ttcatcactt cggatgcaga ctgggtaacg
    40981 ttcacataca tcggattggt ttggcggatg gtggctaaaa cagttgtatc gcccgcattc
    41041 aacagcgtac cttcggaaac tttggactgg ccgataaagc cggaaatcgg cgcggtaatg
    41101 cgcgaacggt tcaggctgat gccggcagat ttgatcgccg cctgcgccgc tttaacgcct
    41161 gcttcggcag aacgtttcgc cgttaccgcc gcatcgtact cttgtttact gatggcatcg
    41221 gcggcaacca gcggtttgta acgcgccaaa tccgcatccg ctttggcaag cgttgcctgt
    41281 gccgttgcca gttgcgcgcg cgcgctttcc agacttgctt cataagtgga actgtcgatc
    41341 tgatacagcg gctgtccggc acggacataa ctgccttctt ggaacaggcg tttttggata
    41401 atgccgccaa cttgcgcacg gacatcggcg gtacgcagcg attccaaacg tcccggcaac
    41461 tcgacggtca acgcaacggt ttgcggatgg acggtaacaa cgccgacgac gggagccgga
    41521 gcttcccgac cagcaggctg cccgccctgt gccgcttcat ctgctttgcc gcaagatgac
    41581 agtgccaacg cgacagaggc agccaacgcg gccgcacgca tcgccttaga agcataaaaa
    41641 gccattatct atcctatctg tatgatttta ttaaaggatt ttagatttaa cagaaattcc
    41701 cgtttcaaaa tacaaaaccg cctgcttcgg catccctgta ttcaaacggg ctgcaaagca
    41761 ggtgggttcg gcacttcggc actgggaaac tgtctcaatt tcaggggcgt tattatacat
    41821 acacgattac acggatacaa agtctttttt ataatcccaa ccgtcaaacc gcgacggaac
    41881 gaaaacgcca ttatgagaaa aaccaaaacc gaagccttga aaaccaaaga acacctgatg
    41941 cttgccgcct tggaaacctt ttaccgcaaa gggattgcgc gcacatcgct caacgaaatc
    42001 gcccaagccg ccggcgtaac gcgcggcgcg ctctattggc attttaaaaa taaggaagac
    42061 ttgttcgacg cgctgttcca acgtatctgc gacgacatcg aaaactgcat cgcgcaagat
    42121 gccgcaaatg ccgaaggagg gtcttggacg gtattccgcc acacgctgct gcactttttc
    42181 gagcggctgc aaagcaacga catccactac aaattccaca acatcctgtt tttaaaatgc
    42241 gaacacacgg agcaaaacgc cgccgttatc gccattgccc gcaagcatca ggcaatctgg
    42301 cgcgagaaaa ttaccgccgt tttgaccgaa gcggtggaaa atcaggattt ggcagacgat
    42361 ttggacaaag aaacggcggt catcttcatc aagtcgacct tggacgggct gatttggcgt
    42421 tggttctctt ccggtgaaaa tttcgatttg ggcaaaactg ccccacgcat catcgggata
    42481 ataatggaca acttggaaaa ccatccctgc ctgcgccgga aataatcaag ccttggtaac
    42541 aatgccgtct gaaacaaaca aaccctttca aacggcatca aaatgacaca aagcattctt
    42601 ctaaaaatac atattcacta aattgcattt ttaatttccc ctatcatcgc atgaacattg
    42661 tcttggtcaa aatgtccgtt ttcttctgaa taaacttcta acaaataatg ttcaatgaac
    42721 gttttatctg tcatcaacga tacatctttg gcaatgtctt catacgactc aaaatcatct
    42781 tcatgccatg gatcatattt atccatgatt ttttgaattt catttttcat atcatttacc
    42841 ttccaatatt tatttacaat taataacaat accattcgaa tgtaaactgc atttttctcc
    42901 ggcattcttg caaacaaaaa ccgaaaatcc cgtcattccc gcgcaggcgg gaattcgttt
    42961 ttttgagttc cagtcattcc tgataaggct ttaacgtcaa gttttcggat tcccgccttt
    43021 atgaaaataa cgatgtgggc attttccgtt ttaatctatt acggttatat acatatacga
    43081 ttattttagt ttgcttacaa aacacttcat gttaaattca aaaatttaat gcactcaata
    43141 tattttttta aggagaagca gatgagtcaa accgacgcgc gtcgtagcgg acgattttta
    43201 cgcacagtcg aatggctggg caatatgttg ccgcacccgg ttacgctttt tattattttc
    43261 attgtgttat tgctgattgc ctctgccgtc ggtgcgtatt tcggactatc cgtccccgat
    43321 ccgcgtcctg ttggggcgaa aggacgtgcc gatgacggtt tgattcacgt tgtcagcctg
    43381 ctcgatgccg acggtttgat caaaatcctg acgcataccg ttaaaaattt caccggtttc
    43441 gcgccgttgg gaacggtgtt ggtttcttta ttgggcgtgg ggattgcgga aaaatcgggc
    43501 ttgatttccg cattaatgcg cttattgctc acaaaatccc cacgcaaact cactactttt
    43561 atggttgttt ttacagggat tttatccaat acggcttctg aattgggcta tgtcgtccta
    43621 atccctttgt ccgccgtcat ctttcattcg ctcggccgcc atcctctcgc cggtctggct
    43681 gcggctttcg ccggcgtttc cggcggttat tcggccaatc tgttcttagg cacactcgat
    43741 ccgctcttgg caggcattac ccaacaggcg gcgcaaatca tccatcccga ctacgtcgta
    43801 ggccctgaag ccaactggtt ttttatggta accagtacgt ttgtgattgc tttgattggt
    43861 tattttgtta ctgaaaaaat cgtcgaaccg caattgggcc cttatcagtc agatttgtca
    43921 caagaagaaa aagacatccg gcattccaat gaaatcacgc ctttggaata taaaggatta
    43981 atttgggcag gcgtggtgtt tgttgcttta tccgccctat tggcttggag catcgtccct
    44041 gccgacggta ttttgcgtca tcctgaaaca ggattggttg ccggttcgcc gtttttaaaa
    44101 tcgattgttg tttttatttt cttgttgttt gcgctgccgg gcattgttta tggccggata
    44161 acccgaagtt tgcgcggcga acgggaagtc gttaatgcga tggccgaatc gatgagtact
    44221 ttgggacttt atttggtcat catctttttt gccgcacagt ttgtcgcatt ttttaattgg
    44281 acgaatattg ggcaatatat tgccgttaaa ggggcggtgt tcttaaaaga agtcggcttg
    44341 gccggcagcg tgttgtttat cggttttatt ttaatttgtg cttttatcaa tctgatgata
    44401 ggctccgctt ccgcgcaatg ggcggtaacc gcaccgattt ttgtccctat gctgatgttg
    44461 gccggttacg cgcccgaagt cattcaagcc gcttaccgca tcggtgattc cgttaccaat
    44521 attattacgc cgatgatgag ttatttcggg ctgattatgg cgacggtgat gaaatacaaa
    44581 aaagatgcgg gcgtaggcac gctgatttct atgatgttgc cgtattccgc cttctttttg
    44641 attgcatgga tcgccttatt ctgcatttgg gtatttgttt tgggcctgcc cgtcggcccc
    44701 ggcgcgccca ctttgtatcc cgcaccttaa acacgataaa caaaataccg tctgaaaacc
    44761 ttttttctgt tttcagacgg catttgcctt tccatcccgt caggcttctc cggtcccttc
    44821 cctcttttcc gctgcggcaa gcgtgtcggc gagcaggcgg gcgaggtttt caaacagggg
    44881 ctgttcgagc gggttttggc ttgcgttgcc gaacacgagg gcgacttcgg tatagtcttt
    44941 ctgccctttg ctgactttgc tgccgtaata ggcggtttgg gcttttttca gggcggcttc
    45001 ccaatcttgt ttttgggcaa acgcttcggc ggcggcaagc gaggttttgg caaaaaacgg
    45061 cagcgggcgg ttttgcccga tattgaaaaa cgccatccat tccgacaaca gctgcaatgc
    45121 cctgtcttgc gcgatttccg ccaatacttc tgtttctccg gattggacga taaaggtttg
    45181 ccgcgtttcg gcttcagacg gcataacggc gcaaaatatc aggtgttcca acaaaaaagc
    45241 cacacgttgc ggcgcattgg gtttgccgta ggcgtaaaac acctgaccgc agcggtacag
    45301 attgcccaag ctgcctttca ggatttgccc gtccgacggt atggcgtatg ggaacggagg
    45361 aagttttggg ctgtttaaaa ccgccgtgtc gatttgtttg gcggcggttt ggaagtcctg
    45421 ctgccaaagt ctgcccaact ctcccgacgg caggaggctt tccgccccga tgcgggcggc
    45481 ggtttgggca aaatcttgtc cttcgcgccg tgcttcgatg taggtttcgg cgatttgatc
    45541 ggcgtgttgc ggatcgaagg gttcggcagg ttcccaggct tcgccgatat ggggttcgct
    45601 ccacgcaagc tgctgctgaa gccatacttt gacggggttg cgccagaagc ggataaattc
    45661 gtcctgtccg atttcggcaa cgggttcggc gttttctacg ggttggtcga aaaaagggcg
    45721 cggcggttcg ggcgtttgtc cgagcgcggc ggcgtagtcg gtacgcgtgc cgaatatgcc
    45781 gtctgaacgt ccgccttctt ggaaatatcg gcgcgagaag gcttgcagcg gatgctgttc
    45841 tatccaattt tgtgcaagtt ggcggctgtt tgtgccggtc atggcggcaa ctgtgtcgat
    45901 gagttcgccc aacagggaag acggggcaag ctcttcgtct ttgcggatgt cgcgcccgat
    45961 gtaggacaaa tacaggattt cgcgcgcgct gatgagggct tcgaggaaca ggtagcggtc
    46021 gtcgtcgcgg cgggcgcggt cgcctttggc gggatgtttg gcaaccaggt cgaatacggc
    46081 ggctttggta ttgcggggaa aatctccgtc gttcaaaccc aacaggcaga tgactttgaa
    46141 cggcaggctc cgcatcggca ccatactgca aaaggtgatg ccgccgcgta aaaagcctgc
    46201 ctcgctttcg ctgtcgagga agcggcggat atggcggatg acggtttgtt gcggcaactg
    46261 tccgaaaaac ccagccaaag tgctctcttc ctgccatttg acccattcgt tttcgaggtt
    46321 ttggactgcc ttttggtcat cgggcccggc ttgaaacaat gtttcaagca aatcccggca
    46381 acgcgccacc cattcgccga ccgtcgcagg ttgccgccat atccgggcaa tatccgacag
    46441 ggtttcgagg aaggcggcaa aacgtccgaa cagggcggtt tggttcacgt cggcatacca
    46501 cgcgctgaca tcctgccaca tcgggttgcc gccttcgggc agcatccagc ccaatatcat
    46561 acggtctgcc gcctgcttcc aggtaaacag ctgatccgtg ccgccgcgca tttctccgtc
    46621 caaaccccag cggacgttca aatcggcaac catgtcgtgc aaaagcggta aatcgtcccc
    46681 ggtcagtccg aaacggcgca acacgggcgc ggtttccaaa agcgcgagca ctttgccgac
    46741 ttcaaaacgg ctttccagca agtcggacac gcactccaac gcataaaaaa acggttgccg
    46801 gcggctgatt ttcacgtccg aaacggaata cggcaatgcc tgcgcgccgg gctgcgcctg
    46861 tccgaacacg gcttcgataa aaggcgtata gggttcgatg ttcggggtta atacggcgat
    46921 atcatgcggc tgccaatcgg gatgttcatg cagaattttc aacagcttgt ctttgagtat
    46981 ctgcaattcg cgcaaagggc tgtgtgcgga gacgatgcgt atcgagccgt cgcccgtgtt
    47041 gacgcttccc acattttcag acggcatttt caggttttga atatcggttt gcagggcgtg
    47101 caaaagcgta tcgcgtccgc cttcttcaaa taccggcgtt tcgccttcta tttccatttc
    47161 gttcaaaaag tcgaaaaagt cgcgcccctg cttgcccaat gaggcaagca gcggatgccc
    47221 tgcctgcgtc agatcgggat cgccgccgcc tttgaggatt tgcgccgctt cgatgacgtt
    47281 gccccaatgc atcccgctcg gattgagtgc gaacacgaac acgtcgcaat gttcggacag
    47341 cttgtgcaaa agctgcaaat acatcggcgc catcgtggaa atgccgaaca cgaaataacg
    47401 ctcgggcagc ttatccttgt ccaacgcggc aagcagtttt tcccacaacg cgacacggtg
    47461 cggcgcgctc tgcctgccgt cgtcgaggta acgccacagt ttggactgcc agatttcgtc
    47521 gtcgcccaaa ccgagtatcc tgccctgctg ccaagcgtct atccactgag gacggtacac
    47581 gaggtattgg tcgaatatgt ctgcaagctg tcccgcaagc tggtaatccg ccgattctcc
    47641 gctgcccaga tagtcttgca gcacattcct cacatcttca aattccgctc cgttttgaaa
    47701 ttctgcgctg cggaacaaat ccagcagccg ccagcgcatg acttcgggtg caaacgggct
    47761 gagttccgga ataccgggaa tcagtttttt catcaacttc cacgtcaggc cggcgggcag
    47821 gctgaacgcc aaattcgccg ccacgcccaa atcgcgggcg aggcaggtgt tgaggtagcg
    47881 gcgcatcccc tggctctgca caacaatctg ttcgggctgc aaggcacatt tcagcggttt
    47941 gactttttga atgcgggcaa acagtgcagc gagcgattcg aggcggttgg attggtacag
    48001 gtaaaacatg gcttgctttt tcaaaatgac agtgatgcgg aattatatag cttctcgggc
    48061 aagccgcccg caaacaaaat gccgtctgaa cgcttcagac ggcatcgggt catttaaacc
    48121 atttcctcaa cacaggaatc cgcgacagca gcaaggtatc caacagccaa atcacggcaa
    48181 tggcaagcag cgcggtcggg aaaagcagtg caatcaacag cagcggcaat gccatcgccc
    48241 accagacggg cagtttgatt ttttgcgccg gaggaacaat gcccgccacg ccggacggac
    48301 ggcgtttcca ccacatcacg cagccgctga tgccgataaa aatcacggca aggcagaaca
    48361 cgacgttcgc caacacgctc caccagccca aagtccccat atgcagcgca atgcttgccg
    48421 ccataaattt gccgaacggg ttgtaatcgt caaaacggat gtcggcaaga atcttgccgc
    48481 tgtactggtc gatatgcacc gtgcggtcgg caaacgggct gatcatgtca taactcatag
    48541 aatcctgcga caaagtccat accccgtcct cgcctttggg caaattcaac tgataacgcc
    48601 ctttgaaacc gatttcccgc gcgaaacggt cgacggtttc caatgtcatc ggctcgctgg
    48661 ggttaatgcc gttttcaccc acagtcgtcc ctgagacagg cataggcgta agctccaaaa
    48721 tccacggcac ttccttaacc ttgccgtcat tcaatacctc gccgtgggtc ggcacgactg
    48781 aaacggggtt cggttcgaca ccccatttgc cggccgggaa ctgactccaa gcctgcacga
    48841 acttgccgcc ccaaatacct gcccaagcaa tacccgacag gcagaacaac agtaaaatca
    48901 acgataccca agttccaaac gcgccgtgca gattccgcca ccaagaacgc gccctgcctt
    48961 ttggcggcag cagcatcgct ttaatgccgc gctgtttcgc ccaccaaagg tacaagccgc
    49021 tgacaaccat aataatggtc agtgaagctg ccgtttccaa aagataatcg cctgccgcac
    49081 cgagcatcat atcgccgtgg atttcatcca tagtgtgata ccaaccctga ttgcgcggca
    49141 tggtgttgac cacttttgcc gtataaggat cgaccgcaac catggttgcc ttaccctcat
    49201 tgttgacacg gaacacagca accatatcat cggcacgcgg tgcaatatat tggacgacgg
    49261 acgaagtctc cggattaatg gcaccgcgtg ccgcttctgc ctgaacagag aggggttgca
    49321 ccactgcctg agggaaaacg tgaatacgct ctccctcttt accggtaata ttggcaaaca
    49381 acagcatacc caaacctgta acggcaagca gggtcaagaa gggcataacc aataaaccgg
    49441 cataaaaatg ccacctccaa acgctcagat aatgccggtt attctgctga ccggcttcag
    49501 ttttgatttg tgtatccatt aatcgtcctt ttgaaattaa ggctatcgtg atgatgcgcg
    49561 attataaaca ataaagacta attctttatg actaaaatca atttttttgc aacatatgaa
    49621 aaaagattca attttgaaga aacaaaccta tatgccgtct gaaagcatca aacaatgctg
    49681 tccgacaccc aactgcacca ttctttaaac catacaaaaa tatcagtcgg tctgactgct
    49741 taagcatcaa aatccccact ttcagacggc ataaatcaaa aacaggattt ccgttcaaaa
    49801 taaaaaatcc ggattcgata tgaatccgga tttctgcatc cgacaaagtc ggaaaacaac
    49861 ggttagaact taccgcctga ctggttgacc atatagtcaa ccgcagcttt gacctcatca
    49921 tcgctcaaat cgccgcgacc tccttttgcg ggcatcgtat taaaaccttc gatcgcgtgt
    49981 ttgtgcaacg tgtccttacc ttttttgatg cggtcggccc agtcggcttt ggtgcctaca
    50041 tggggaatac ctggaattgc attgccatgg caggcggcac aaacggtttc ataaacctgt
    50101 ttgccatccg ctttggcggc aggtgcggct ttttcctcgg ctttaggttc tgctgcggca
    50161 ggtttggctt cagaagcggc ttgtgctgct tctgcaggag cagatggtgc gggttctgcc
    50221 gccggtgcag gctcggcttt tttcaccgga gctttaccgt ctttattgga aagaccccat
    50281 acataagcag tcatgatatg cagtttgtct ttatccaaga aatgtcccca agcgggcatt
    50341 tggctgctgc gaccgttggt aatggtttcg ataatggatt tttgcgtacc gccccacaac
    50401 cacacgtcat cagtcaggtt cggacccaaa ccttggatac cttgtccctt atcgccgtgg
    50461 caagtgaaac agttggcagg cggaccgctg aacaaggctt gtccgcgcgc ggcacgttcc
    50521 tcatcatact gacctttggg ttttgaaagg gacatcacat aatgggcaac gtctttcacg
    50581 ccttcttcgc ccaaagcagg accccaggca ggcatagtcg caacacggcc tttttcgatg
    50641 gtctcgtgga ttttatcggg atcgccgccc cacaaccaat cgctatcggt cagattcgga
    50701 aaacctttag aacctttggc atcagagccg tggcactgga tacaataagt gttaaacagg
    50761 ttttgggcga tttgcttggc ctgtgggtct tttgccactt tttcaatcgg catatccgca
    50821 aacttggcat acagtttgcc gtattgctca tcggcttttt tgacctcttt ttcatattgg
    50881 ttatggctgg tccatttcag cagacctttg tagtcgccga cacccggata cataaccaga
    50941 taaccgatac cgaacagcca cgtcaaaaca cacagccaaa accaccagcg gggcagcgga
    51001 ttgtcgtatt cggcaatgcc gtcccactcg tgacccgtag tttgtacttc ttcgcccttc
    51061 ttcggacgtt tgacaacatt ttgagacagc agcagccaag ccaaagcgat aaagctcagt
    51121 aagacaataa ctgcaatata tatattccag aaattactgg taaattggga tgttgcgttc
    51181 attgttttgc tccgttatca caatattaac ggttttcgct tttcttatct tgcgcatctt
    51241 ggttttcagc aaaaatgctg tttgcggcat catcgtagtt tttcttattc cgcctgttga
    51301 agacgatata gagtactaac aggaaacaga taaagatcca taccgtgaaa agagcgcgaa
    51361 taccgttaat atccatgatg ttaccttacg tttttcaaag ccagacccaa tccttgcaga
    51421 taggcgacta cagcatccag ctcggatttg tttgccaaag cctcaggcgc tttcgcaatt
    51481 tcctcatcac tgtaaggagt acctacttta cgcaaagcct tcatgttggc aacggttgca
    51541 tcgacatcga ctttattgcg tgcaagccac gggaatgccg gcatattgga ctcaggcacg
    51601 acatcacggg gattcagcag gtggatgcgg tgccattcgt cggaatagcg gccgcccaca
    51661 cgtgccaaat caggaccggt acgtttggaa ccccattgga acggatggtc gtaaaccgac
    51721 tctccagcaa cagagtaatg accgtaacgc tcagtttccg cacggaacgg acgaatcatt
    51781 tgagagtggc agttgtaaca gccctcacgg atgtaaatat cgcgtccggc aacctgcagg
    51841 gcattgtaag gcttcacgcc cggcgccggc tgtgttgccg ccttggtaaa ggccaagggc
    51901 acaacttcaa tcaacagacc gacactgact acaagcagcg tgaacacaat cagaacgccg
    51961 attttttctt cagccaattg ttgtaatttc attttggtag cctatctttc ttagtatttt
    52021 agtggtgctg tgtttgggaa accgcaggga tttcggcatc gactgcttta ccaccgatgg
    52081 ctgtgcggta cacgttgtac gccataatgc acatgccact cagatacaat aaaccacctg
    52141 caaaacggat catgtagtaa ggcatggtgc gttttacgga ttcgacaaac gagtaggtca
    52201 gcgtaccgtc atcgttcaga gaactccaca tcaaaccctg catcacacct gcaatccaca
    52261 tcgcggcgat atacagaacc acaccgatag tcgcaatcca gaaatgcgcc tctaccagct
    52321 tggtgctgtg catctgctct ttgccgaaca gacggggaat catgtaatag acggaaccga
    52381 tggttacaaa gcctacccag cccaacgcac ccgcatgaac gtgcgcgacg gtccagtccg
    52441 tatagtggct caatgcattg accgttttaa tcgacatcat cgggccttca aaggtagaca
    52501 taccgtagaa ggacaaggat acaatcagga atttaagaat cgggtctgta cgcagtttgt
    52561 cccacgcgcc ggacaaggtc ataatgccgt taatcatacc gccccaagag ggtgcgaaca
    52621 aaatcaagga cagaaccata cccaaagatt gcgtccagtc aggcagcgca gtgtagtgaa
    52681 gatggtgcgg acccgcccac atataggtga aaatcaacgc ccagaagtga acgacggaca
    52741 ggcggtagga gtaaacgggg cgggctgctt gtttgggtac gaaatagtac atcataccca
    52801 agaagccggc agtcaggaag aagcccacgg cgttatgccc gtaccaccat tgaaccatag
    52861 catcaatcgc accggaatag acggggtatg acttcatcaa accggccggg atgctgatat
    52921 tgttgacgat gtgcaaaagt gcgaccgcca aaataaagcc gccgtagaac cagttggcaa
    52981 cgtaaatgtg ttttaccttg cgtttggcaa tcgtaccgaa gaatacgatg gcgtaagcca
    53041 cccaaaccaa agtaatcaga atgtcaatcg gccattccag ttcggcatat tccttacctt
    53101 gggtccaacc catagggaag ctgacgacgg cggcaacgat taccgcctgc caaccccaaa
    53161 aggtaaatgc cggcagccaa ccgccgaaaa gacgggtatt acaagtacgt tggacaacgt
    53221 agtatgatgt gccgatcaga ccgcaaccgc caaatgcgaa aataaccgca ttggtgtgca
    53281 gcggacgcag gcggccgaag tggaaccaag gtccgatatt agacaagtcg agggcaggag
    53341 caaaaagctg ggcggcgacg ataacgccga ccaacatgcc cacaatcccc caaactacag
    53401 tcatgatggc gaactggcgc accactttgt agttgtaagt ttgtgtgtcc atgagagtct
    53461 ccatgaatta tgggaataaa gatttttatc ctgccgcttc cgcagcctgt ttaaggtgca
    53521 atccgggcaa gcgtaatttt ttctaaattt aacatatctg ccttattacg ccaagcggaa
    53581 ttacattcgc gccgccggcg aaccctttgc ttaatctgtt ttttattaca tataaatcat
    53641 attgttataa taaattacaa cccggccgcc attgcttttg tttccaattt tccctttttg
    53701 tggcacttta ttgatgtagg ttaagctgca ttttaaaggc atttaatcca tcccgtttaa
    53761 cgatatattt gatagttatg attcattata aaatagcccc gtcccctcta gaccatgaat
    53821 ggcacatcct gctgaaattc acacaagata atgattttcc tatagaaata agccttccga
    53881 attgggtgcc gggcagctat ctgattcggg atttttcccg ccacatcact tctatccatg
    53941 catcctgcaa cggcacgtcc atgccgctcg aacaaattgc aaaaaaccgc tggcacactg
    54001 ccgccgtacg cggcgagtgg caaatccgct acaccgtata tgcattcgat ttgtcggttc
    54061 gaggttcttt cctgacgaca gaacgcggtt tttttgacgg atcgtgcctg tttttgaaag
    54121 tcgaaggaac ggaaacgctg ccgcaccgct tggaattgac aggcattcca cccgaatggc
    54181 gtattgccac aacgctgccg gaaacaggga ggcttgtctt tcagacggca tcttatgacg
    54241 aattgattga ccgacctgtc gagatgggct tgattgaatt tttagatttt gaggcggcag
    54301 gcattccgca cacgattgcc ttaaacggca tatatcccga tttcgaccgc gacaggctgg
    54361 tttcggacat caaaaaaatc tgcgaaacag aactggcgat gttctcctcc cctgccccat
    54421 ttgagaaata tttgttcctg ctccacgtcg gcgaccatat ttacggcggt ttggaacaca
    54481 ccgacagcac cgcactgctc gccgatcgcc acagccttcc gccgtacggt atgaccgatg
    54541 ccgacgatgc ctacaccaca ttgctcggac ttttctccca cgaatatttt cacgcgtgga
    54601 acgtcaaatc catcaaacct gccgcattcg ccccttatga cctcgacaaa gaaaactata
    54661 ccgaacaact atgggcattc gaaggcatta catcctatta cgacgatttg tttttggcac
    54721 gcagccgcac catctcgccc gaattttatt taaacctgct agcacaaggc attacgcgcg
    54781 tacaacaaac ccgcggccgt ttgaggcaga ccttggcgga atcgagtttt accgcgtgga
    54841 acaaatttta caaaccggat gaaaacagcc ccaacgccat cgtcagctac taccaaaaag
    54901 gcgcacttgc cgcattgtgc cttgatctga taatacgcaa ccgaagcaac ggcagacatt
    54961 cccttgatac ggtaatggac aaactctatc gggagtggag ggacacacac tcgggtattc
    55021 cggaaaaaca ctggcaaatc cgttgtcagg aaattaccgg cttggatttg acagattttt
    55081 ttcagacggc attgtacagc accgaagatt tgccgcttgc cgaatgcctg gcaaccacag
    55141 gcgtgaaact gaccttcctg ccgcttcccc gacaacacgg cggcggatat gcggaacaca
    55201 ttttccccat cccgccgacg ggcgatttcg gcgcacgctt caaacaaaac gccgaccata
    55261 tcgtcctgac ccatgtcttc aacggcggaa gtgcggaatc tgcggcactg tgcccgcaag
    55321 acaaaatcat tgctttagac ggttatgcct gcaccgactt tgccgcacaa tgggcccgat
    55381 accacgtcag ggcaaaaatc aatatccact tcttccgtgc aggcatattg cgtcaaaccg
    55441 tcttgacggt tcaggcaacg gcggcggata ctgccatcct acacatcaca gatcgcaacc
    55501 tgttggaaaa ctggttgttc ggttaaactt tcagacggca ttgcacacaa aatgccgtct
    55561 gaacaccaac cgcaaagcaa aggaaacaaa atggcgattc tcaaacttga cgaacatctc
    55621 tacatctccc cgcaactgac caaagccgat gcggaacaaa tcgcgcaact tggcatcaaa
    55681 accgtcatct gcaaccgccc cgaccgcgaa gaagaatcgc aacccgactt cgcccaaatc
    55741 aaacagtggc tggaacaagc aggcgttacc ggattccatc accaacccgt taccgcacgc
    55801 gacatccaaa aacacgatgt cgaaaccttc cgccaactca tcggacaagc cgaatatccc
    55861 gtccttgcct attgccggac cggtacgcgc tgctccctcc tgtggggctt ccgccgggcg
    55921 gcagaaggta tgccggttga cgaaatcatc cgccgcgccc aagcggcagg tgtgaatctg
    55981 gaaaacttca gagagcggct ggacaacgcc cgcgtctgat tacaagccga aacgtttaaa
    56041 ccacaccttc aagcggcatt ccaccgcaac ttgaaaaaga ggacggcaaa ccttactgcc
    56101 gtcctctgtc cttctccgtt tttacagtgg gtatagcgga ttaacaaaaa ccagtacggc
    56161 gttgcctcgc cttaactcaa agagaacgat tctctaaggt gctgaagcac caagtgaatc
    56221 ggttccgtac tatttgtact gtctacggct tcgttgcctt gtcctgattt ttgttaatcc
    56281 actataaaaa ttagaaatgc acattttcat tattctcgcg caggcaggac tccagactta
    56341 cccatttcag taatgtttga aaataaaaga aaaatcagat gtttgtattc ccgcctgcgc
    56401 agaaatggag acggtgctct gtcgtctcat ttttgtttta atcaactata tataactgat
    56461 taaacataag aaatgccgtc tgaaagactt tcaggcggca ttcgttcaag cgtcgaactt
    56521 tattgcgcct tggtttcggt tacaaaaccg attttggtaa ttcctgcctg acgggcggct
    56581 tctaaagctt tgtttacata atcgtattcc accgccttgt ctgccgcaat cgccacaatc
    56641 acgttttcat tctgctcctt ggcggctttc agacggcttt ccacttcccc gatttccact
    56701 ttgcttgcag aatccccgcc gacataatag ccgccgttcg catcaatcgt caggcgcagg
    56761 gggtctttag gctgtttgtc ctgcttgttt gcctgctcgg acgcggtcgg cagttccaaa
    56821 gggatggaat gcgtcagcac cggcatagta atcataaaca caatcagcaa caccagcatc
    56881 acgtccacca acggcgtaac gttgatgtcg gacatcggag aatcgtcgct ggaattcatc
    56941 gaaccaaatg ccataatcag ctatcctttt gattaagcag gcggacgtgc aaatcgtgcg
    57001 ccatcgcatc caaatcctgg gtcagtattt ttgtgccgcg attgaggaag ttgtatgcca
    57061 acaccgccgg aatcgccacg aacaaacccg ccgccgtcgc caccagtgcc tcgccaatcg
    57121 ggccggcaac cgccgcaata ctcatctgcc cgctttgccc gatattgatc agggcgtggt
    57181 aaatccccca aaccgtgccg aacaacccga taaacggcgc ggtcgcgccg atggaggcaa
    57241 gcgcggtcat cccgtaatca aaccggcgca taatctgcgc catactgttg cggatttgaa
    57301 tgaccaaata ctcgttcaac ggcaaagcct gcgccagttc ggacgcttcg tttcggcggt
    57361 agttgcggta agactgcaat gcctcttgcg ccagtttgga caaaggcgca tcgacggcgc
    57421 gcactttttc gaccgcgtcg ttcagcgaca aagtatcgcg catatgccgt ttgacggcgg
    57481 cattcccttt gcgcgcccga tacagcttga tgcagcgcaa gacaaccaga caccacgtta
    57541 cggtactcat caacagcatc aacacaaaca caccgatcag gacgggatcg cccgattcaa
    57601 acactaattt caaattcata atgattccaa cactgaaaaa accaatcaaa catccaagct
    57661 gccgcaaact gccgcgacaa ccgcctaatt caattcaaac ttgacgggga ctttaaactc
    57721 cgtccaggca ttggcttgaa aatgcccgtt ttgcgccgct ttgcgtgccg cattgtccaa
    57781 acgggaaaaa ccgctgcttt tcaccacttt gacggactcg acatgaccgc ccggagaaac
    57841 caaaacgctc aaaacaacca taccctgctc gtcattttcc atagaaagtg cgggataggc
    57901 cgggcgcggg atgctgccgt tggcgcgtaa aggattgcct ttgctgctgc cgacaccggt
    57961 tccgccgccg ctgccgccgg ctccttcccc gtgttcgcct ttggcaccgc cgcttccttt
    58021 accgctgcct tctccgcgcc ccgttccgtc gcctttggta ccggttccct taccttcccc
    58081 attaccctgc tcgctgccgg ctttggcaga agcattaccg gaatgttcgg caggtttttc
    58141 agacggcttc tctgccggtt tttccgccgg tttcggggca ggcttcgctt ccggttcggg
    58201 ttcgggctta ggtttttctt cgggtttcgg cttttcctta ggttgctgaa tatccgcatc
    58261 cgcctttttt gtaaccgccg gcttcaaaac cggcttgggc ggttcgacag gtttgggcgg
    58321 atcgggcgcg ggttgcggtt cgggcgcggc aggcgcgcct gcgccttcgg gtgcgccgcc
    58381 ccctccgcca aaatcgccca aatcgacaaa ttcgataaca tttcctgact ctatcacggg
    58441 cagcttgtgc gcctgccaga gcaatgccac tattgccaaa tgcagcagtg cgacggaaaa
    58501 cacgactgcg ggggttaaaa ttcgttcttt atccataatt cgggcataat aatagcaaca
    58561 attcctattt gcaacctatt ttttataatt tgttgtcatg tgaacgcctg ctccattcat
    58621 aggcaaaccg tgtttaaacg cggtattaca gcaaatcatc agataacggg ccggcagaaa
    58681 aaatgattcc gtctgatttc ttattccaat aaaatcaggt tagatgatat attgccgctt
    58741 ctgtctgtca gccgtttcgg gctgcacacc acatctgttc aaaggaaaac catgtttcaa
    58801 aattttgact tgggcgtgtt tctgcttgct gtcctgcccg tgctgctcgc cattaccgtc
    58861 agggaggtgg cgcgcggcta tacggcgcgc tactggggag acaacactgc cgaacaatac
    58921 ggcaggctga cactgaaccc cctgccccat atcgatttgg tcggcacaat catcgtaccg
    58981 ctgcttactt tgatgttcac gcccttcctg ttcggctggg cgcgtccgat tcctatcgat
    59041 tcgcgcaact tccgcaaccc gcgccttgca tggcgttgca ttgccgcgtc cggcccgctg
    59101 tcgaatctgg cgatggctgt tctgtggggc gtggttttgg tgctaactcc gtatgccggc
    59161 ggggcgtatc agatgccgct ggctcaaatg gcaaactacg gtattctgat caatgcgatt
    59221 ttgttcgcgc tcaacatcat ccccatcctg ccttgggacg gcggcatttt catcgacacc
    59281 ttcctgccgg cgaaatattc gcaagcgttc cgcaaaatcg aaccttatgg gacgtggatt
    59341 atcctgctgc ttatgctgac cggggttttg ggtgcgttta ttgcaccgat tgtgcggctg
    59401 gtgattgcgt ttgtgcagat gttcgtctga ctggctctca gacggcataa acgttccaaa
    59461 aaacgcggaa ggacatattg ccctgccgct tttttttggt tttacaatgc cctgtcgaaa
    59521 taaagcggca cgcctgattt ttcactcata ccgattaaat aatccatcgt cttccgaggg
    59581 aacgcgatgc cgcgcaccac ggtcagattc ctcaaaacgg ggaaaaccaa aatatcctcc
    59641 ataccgattc caccgttgat gccgtctgaa gtaccgtcca tcaaattctc caactcttgc
    59701 aaatctgcgt ttatccgttc aaggtattgg gcggttttat tcaaattggc ggaaaagctg
    59761 ccgatgcttt tctctttttt gtctgtgaaa tatttcaccg cttccggcgt tacaaattcg
    59821 ggcagcccga ttttgatcaa gcgtggctga accaatttgt tgttgtatcc gcctaccttg
    59881 tccagccacg cctgtatctc ggggcggact tcgtctttca gacggccttc tcggtcgaaa
    59941 tggcacacaa tgtccaaact ctcgcccata aacgaaccgt cttctttttg caggacgggc
    60001 acttgtttcg caccgatcat accgatcggc gttgcctcgt cgtcgtttgc cagcacgact
    60061 tcttcaacgt cagcaccaaa cagcccggca gccatccgcg cacgcacgca aaacgggcaa
    60121 tggtcgtaaa tatacagttt catcaaaata ttcctcgtca acctgtcggt accggctacc
    60181 ttaacacccg gcgccgcccg aaacaagttt atcttcccgc ctatgcaccg taaataaata
    60241 agctgttaca atgaactcgt ttttatcgga acggaagatc ccatcatgac cgccatcagc
    60301 ccgattcaag acacgcaaag cgcgaccctg caagaattgc gcgaatggtt cgacagctac
    60361 tgcgccgctc tgccggacaa cgataaaaac ctcatcggta ccgcatggtc gctggcgcag
    60421 gaacattatc ctgccgatgc cgccacgccg tatggcgagc cgctgcccga ccacttcctc
    60481 ggcgcggcgc aaatggtcga cgaactcgac ctgctgcccg atgccgtcgc cgccaccctg
    60541 cttgccgaca tcggacgcta cgtccccgat tggaacctat tggtttccga gcgctgcaac
    60601 agcaccgtcg ccgagctggt caaaggtgtg gacgaagtgc agaagctcac ccacttcgcc
    60661 cgggtggaca gcctcgccac ttccgaagag cgcgcgcagc aagccgaaac catgcggaaa
    60721 atgctgctgg cgatggttac cgacatccgc gtcgtattaa tcaaactggc gatgcgtacg
    60781 cgcaccctgc aatttttaag caacgccccc gacagccccg aaaaacgcgc cgtcgccaaa
    60841 gaaaccctcg acatcttcgc cccgctcgcc aaccgcttgg gcgtgtggca gctcaaatgg
    60901 cagctcgaag atttgggctt ccgccatcaa gaacccgaaa aataccgcga aatcgccctg
    60961 cttttggacg aaaaacgcac cgaacgcctc gaatacatcg aaaacttcct cgatatcctg
    61021 cgtacggaac tcaaaaaata caatatccac tttgaagtcg ccggccgtcc gaaacacatc
    61081 tactccattt acaaaaaaat ggtgaagaaa aaactcagct tcgacggcct gttcgacatc
    61141 cgcgccgtgc ggattctggt cgataccgtc cccgagtgtt acaccacgct gggcatcgtc
    61201 cacagcctct ggcagcccat tcccggcgag ttcgacgact acatcgccaa ccccaaaggc
    61261 aacggttata aaagtttgca caccgtcatc gtcggcccgg aagacaaagg tgtggaagtg
    61321 caaatccgca ccttcgatat gcaccaattc aacgaattcg gtgtcgccgc ccactggcgt
    61381 tacaaagaag gcggcaaagg cgattccgcc tacgaacaaa aaatcgcctg gttgcgccaa
    61441 ctcttggact ggcgcgaaaa tatggcggaa agcggcaagg aagacctcgc cgccgccttc
    61501 aaaaccgagc ttttcaacga cacgatttat gttttgaccc cgcacggcaa agtcctctct
    61561 ctgccaacgg gcgcaacccc catcgacttc gcctacgccc tgcacagcag catcggcgac
    61621 cgctgccggg gcgcgaaagt cgaagggcag attgtgccgc tgtccacccc gctcgaaaac
    61681 ggacagcgcg tcgaaatcat taccgccaaa gaagggcatc cttccgtcaa ctggctttac
    61741 gaaggctggg tcaaatccgg caaggccatc ggcaaaatcc gcgcctacat ccgccagcaa
    61801 aacgccgaca ccgtgcgcga agaaggccgt gtccaactcg acaagcagct tgccaaactc
    61861 acgcccaaac ccaacctgca agagcttgcc gaaaatctcg gctacaaaaa gccagaagac
    61921 ctctacaccg ccgtcggaca aggcgaaatt tccaaccgcg ccatccaaaa agcctgcggc
    61981 acgctgaacg aaccgccgcc cgtgcccgtc agcgcaacca ccatcgtcaa acagtccaaa
    62041 atcaaaaaag gtggcaaaac cggcgtgctc atcgacggcg aagacggctt gatgaccacg
    62101 cttgccaaat gctgcaaacc cgcgccgccc gacgatattg ccggcttcgt tacccgcgag
    62161 cgcggcattt ccgtccaccg caaaacctgc ccctctttcc gacaccttgc cgaacacgcg
    62221 cccgaaaaag tactggacgc aagttgggcg gcgttgcagg aagggcaagt gttcgccgtc
    62281 gatatcgaaa tccgcgccca agaccgctcc gggcttttgc gcgacgtatc cgacgcgctc
    62341 gcccgccaca aactcaacgt taccgccgta caaacccagt cccgcgactt ggaagccagc
    62401 atgaggttca cgctcgaagt caaacaagtc aacgacctcc cgcgcgtcct cgccggcctc
    62461 ggcgatgtca aaggcgtatt gagcgttacc cggctttaaa tacaaaaatg ccgtctgaaa
    62521 gccggataac gcttcagacg gcattttgat tgccgggttt tgctattttt tgttgtaata
    62581 atcaaatcgc acgttgacta tgtctttctc ggtaaaaata taacggagca tcgttgtgaa
    62641 tctttcataa cgttcatgaa ttcccacact atcaggcaac caaggggaag atttaattgt
    62701 aaaaagtttc caatttggaa ccattaaaaa ctcaataatg gtaccgattc caatcacgat
    62761 atcccttggt atatccatcg gataaggata ttttctccta acctcgatta aatcattctc
    62821 cagcttccaa tattcttcat catcccacac cccgtcatca taccatttac caataaatga
    62881 attttcgtca tactcttcaa aacaagggat gtttcttcta aaatccttga actcgcacat
    62941 aataattaat ctccaatacg atttaggttt ttatcaaatg taccgtttct tgtttctttt
    63001 ctgtaatgtt attcatcgta gtaaggttct gtcgaataat tgtctttgcc cccggcaatg
    63061 atagtaacaa ttttcccttt tgcttcccat gcttgtactc ctatttcatc aaactcatag
    63121 acatatgtcg gataagattc atttgataaa taatatttat caacaccgta tgatttaggg
    63181 tgatggaaaa gctgtttaaa atcttcaaaa ttcagaccta ttatattaac gcccataaaa
    63241 tatagctcct gataacaaaa tatcgaaata attttgtttt ttttgacgga aatgagtaaa
    63301 tttgagtcgg gagattcata atattctgtt ccaaactcat caggaggttc atacataaaa
    63361 gtttccagta tgtttttgta ctgtgttata tcagcaccaa aacggaatat tcctacagaa
    63421 gtaaaaggta aaaattcggg agttttaacg accgcgtcga ccatgctctt ctccttttgt
    63481 ttttcgattg gcatttttgg caatattttt gattttttgc ttaatcttta agcgttcatt
    63541 tttggacatt ccgggaataa ttttatttgt taattcagca atttttgatt ccgctgatat
    63601 ttgacttcga ccgccgtctc catgtttttc attcttggag cttcctgttc ttttaggcgg
    63661 acaagaatta tgaacccaaa ccccttccgt ttccgcctga ttacccttga cgaagta
//
LOCUS       DAWTQS010000004        58704 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.004, whole genome shotgun sequence.
ACCESSION   DAWTQS010000004 DAWTQS010000000
VERSION     DAWTQS010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 58704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58704
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.004"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            288..1754
                     /locus_tag="WNA25_000254"
     CDS             288..1754
                     /locus_tag="WNA25_000254"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HFA9823105.1"
                     /translation="MAEATDVVLVGGGIMSATLGVLLKELEPSWEITLIERLEDVALE
                     SSNAWNNAGTGHSALCELNYAPLGANGIIDPARALNIAEQFHVSRQFWATLVAEGKLE
                     DNSFINAVPHMSLVMNEDHCSYLQKRYDAFKTQKLFENMEFSTDRNKISDWAPLMMRG
                     RDENQPVAANYSAEGTDVDFGRLTRQMVKYLQGKGVKTEFNRHVEDIKRESDGAWVLK
                     TADTRNPDGQLTLRTRFLFLGAGGGALTLLQKSGIPEGKGYGGFPVSGLFFRNSNPET
                     AEQHNAKVYGQASVGAPPMSVPHLDTRNVDGKRHLMFGPYAGFRSNFLKQGSLMDLPL
                     SIHMDNLYPMLRAGWANMPLTKYLLGELRKTKEERFASLLEYYPEANPDDWELITAGQ
                     RVQIIKKDSEKGGVLQFGTEIVAHADGSLAALLGASPGASTAVPLMIRLMHQCFPERA
                     PSWEGRLKELVPGYGIKLNENPERADEIIAYTAKVLDI"
     gene            complement(1941..2315)
                     /locus_tag="WNA25_000255"
     CDS             complement(1941..2315)
                     /locus_tag="WNA25_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HFA9823106.1"
                     /translation="MKLLTTAILSSAIALSSMAAAAGTNNPTVAKKTVSYVCQQGKKV
                     KVTYGFNKQGLTTYASAVINGKRVQMPINLDKSDNMDTFYGKEGGYVLSTGAMDSKSY
                     RKQPIMITAPDNQIVFKDCSPR"
     gene            complement(2553..2852)
                     /locus_tag="WNA25_000256"
     CDS             complement(2553..2852)
                     /locus_tag="WNA25_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFA9823107.1"
                     /translation="MKIFENIEDVKAIRKKTGLNQIDFWGKVGVTQSGGSRYETGRKM
                     PKPVRELLRLVHIECIDLAKVNKKDMEIAALLKKHHPDLYAELSKQTKSERKKQS"
     gene            complement(2951..3730)
                     /gene="map"
                     /locus_tag="WNA25_000257"
     CDS             complement(2951..3730)
                     /gene="map"
                     /locus_tag="WNA25_000257"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFA9823108.1"
                     /translation="MNGIIIKTPEEIEKMRELGKLVAEALDYIGQFVKPGITTDEIDK
                     LVYDYHVNVQGGYPAPLHYGNPPYPKSCCTSVNHVICHGIPDDKPLKEGDIINIDLTI
                     KKDGFHGDSSRMFTVGKVSPIAQRLIDVTHASMMAGIEAVKPGATLGGVGYACQQVAE
                     NAGYSVVQEFCGHGIGRGFHEAPQVLHYGKKGQGPVLKPGMIFTVEPMINQGKRHLRI
                     LNDGWTVVTKDRSLSAQWEHEVLVTETGYEILTVSPATGKP"
     gene            complement(3770..4420)
                     /locus_tag="WNA25_000258"
     CDS             complement(3770..4420)
                     /locus_tag="WNA25_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823109.1"
                     /translation="MPSEPPSDGIARHPKSTIKMAKKPNKPFRPTPKLLIRAVLLICI
                     TAIGALAVGIVSTFNPNGDKTLQTEPQHTDSPRETEFWLPNGAVGQDAAQPEHHHAAS
                     SEPAQPDGTEESGSGLPSPAAPKKNRVKPRPSDAARAADSLTGTGTQAENTLKETPVL
                     PTNAPHPEPRKETPEKQAQPKETPKEKETPKENHTKPDTPKNTPAKPHKEILDNLF"
     gene            complement(4428..5036)
                     /locus_tag="WNA25_000259"
     CDS             complement(4428..5036)
                     /locus_tag="WNA25_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HFA9823110.1"
                     /translation="MKPKPHTVRTLIAAVLSLALGGCFSAVVGGAAVGAKSVIDRRTT
                     GAQTDDNVMALRIETTARSYLRQNNQTKGYTPQISVVGYNRHLLLLGQVATEGEKQFV
                     GQIARSEQAAEGVYNYITVASLPRTAGDIAGDTWNTSKVRATLLGISPATQARVKIIT
                     YGNVTYVMGILTPEEQAQITQKVSTTVGVQKVITLYQNYVQR"
     gene            complement(5100..5693)
                     /locus_tag="WNA25_000260"
     CDS             complement(5100..5693)
                     /locus_tag="WNA25_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HFA9823111.1"
                     /translation="MTTLQERVAAHFAESIRAKQEAEKILVEPTVQAAELMLQCLMND
                     GKILACGNGGSAADAQHFAAEMTGRFEKERMELAAVALTTDTSALTAIGNDYGFDHVF
                     SKQVRALGRAGDVLVGISTSGNSANVIEAVKAAHERDMHVIALTGRDGGKIAAMLKDT
                     DVLLNVPHPRTARIQENHILLIHAMCDCIDSVLLEGM"
     gene            complement(5698..6045)
                     /locus_tag="WNA25_000261"
     CDS             complement(5698..6045)
                     /locus_tag="WNA25_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFA9823112.1"
                     /translation="MRLNHKQGTAGEDAALAFLQSQGCTLLARNWHCAYGEIDLIVKN
                     GGMILFVEVKYRKNQRFGGAAYSISPSKLLKLQRSVEYYLQQNRLTNVPCRLDAVLIE
                     GNRPPEWIKNITG"
     gene            6092..6967
                     /gene="rsmI"
                     /locus_tag="WNA25_000262"
     CDS             6092..6967
                     /gene="rsmI"
                     /locus_tag="WNA25_000262"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFA9823113.1"
                     /translation="MFQKHLQKAPDSVVGGTLYVVATPIGNLADITLRALAVLQKADI
                     ICAEDTRVTAQLLSAYGIQGRLVSVREHNERQMADKVIGFLSDGLVVAQVSDAGTPAV
                     CDPGAKLARRVREAGFKVVPVVGASAVMAALSVAGVAESDFYFNGFVPPKSGERRKLF
                     AKWVRAAFPVVMFETPHRIGATLADMAELFPERRLMLAREITKTFETFLSGTVGEIQT
                     ALAADGNQSRGEMVLVLYPAQDEKHEGLSESAQNAMKILAAELPTKQAAELAAKITGE
                     GKKALYDLALSWKNK"
     gene            complement(7002..7532)
                     /locus_tag="WNA25_000263"
     CDS             complement(7002..7532)
                     /locus_tag="WNA25_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFA9823114.1"
                     /translation="MNTNQPAVYDPLTRALHWLTVAGFIGILTTIVLWTIYEEAEWAG
                     SLFGLHKSFGFLMLTVIALRIVWAVANRAKRPQSDSKAAAAGHGILYLLMLAVPVIGM
                     IRQYGGGRGPLKVFGVEVMQGSPEKIEWMANLGNTFHGNLGWLLFAAVVGHVAMVVVH
                     RVQGKDVLYRMTGRVR"
     gene            7811..8965
                     /gene="obgE"
                     /locus_tag="WNA25_000264"
     CDS             7811..8965
                     /gene="obgE"
                     /locus_tag="WNA25_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFA9823115.1"
                     /translation="MKFIDEAKIEVAAGKGGNGATSFRREKFVPRGGPDGGDGGKGGS
                     VWAEADENTNTLVEYRFVKRYQAKNGEKGHGSDRYGAGADDIVLKMPVGTLIRDLDTD
                     EIVADLTYHGQRVCLAKGGKGGLGNIHFKSSVNRAPKQSTPGEEGETRSLQLELKVLA
                     DVGLLGMPNAGKSTLITAVSAARPKIANYPFTTLHPNLGVVRIDENHSFVMADIPGLI
                     EGAAEGAGLGHRFLKHLSRTGLLLHVVDLAPFDETVNPAEEALAIINELRKYDEELYG
                     KPRWLVLNKLDMLDEEEARARTAAFLEAVGWDYPEPDDRFQFDMETPRLFQISALTHQ
                     GTQELVHQINQYLAEKKRIEAEKAEAEKAAANVEIIEQQPKTDTGVFKPE"
     gene            9019..9966
                     /locus_tag="WNA25_000265"
     CDS             9019..9966
                     /locus_tag="WNA25_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFA9823116.1"
                     /translation="MYKTIDLFSGIGGIRLGFEKYGCTNVFSSEWDKYARQVYEANFG
                     EKPFGDINGIDPSDIPDHDILLAGFPCQPFSIAGKGLGFEDTRGTLFFNIAEILKTKQ
                     PKAFLLENVKRLTTHDSGRTFRIVLETLKQLGYTVYFKVLNTLDFGLPQKRERIYIVG
                     FSDNIPFYFPEPINQYRPLGELLENDRDVEPSYFLSDTLKQKRLAALKKAPPTPSIWH
                     ENIGGNVSALPYSCALRAGGSYNYLVVNGVRRLTGREMLRLQGFPDDFEINIPYSQVR
                     KVAGNSVSVPVIEAIAENMLASLSGKVEQKGQLDLLEAG"
     gene            9963..11015
                     /locus_tag="WNA25_000266"
     CDS             9963..11015
                     /locus_tag="WNA25_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HaeII family restriction endonuclease"
                     /protein_id="HFA9823117.1"
                     /translation="MTLEEQQAKEALDGIIKKSRVHLYKPIQIAEILYHDRCIKQLDF
                     LNLDTYRNQSKRWRDEICRRFLGRISTSSAKFQDNLFEKNAIPPEKLAVLGTLNRQSD
                     GGVESYIYKQFFNRFSQMSEALAYVGNTDRYSFQLSEFLNLFWLEPGLKRSIDKIYEI
                     VVYALFDALVSELGITVSIDFPKENLFLWEEYQDFAEKIITMPKNEHLKLPAKIHRVG
                     VTNAADRGLDMWSNFGLAIQVKHLSLDEELAEDIVSSISADRIVIVCKKAEQSVIVSL
                     LTQIGWKSRIQNIVTEDDLISWYEKALRGQYPIAEALLENIKTEIMREFPAVNEANEF
                     LDFAQNRGYDITVTHF"
     gene            11031..12452
                     /locus_tag="WNA25_000267"
     CDS             11031..12452
                     /locus_tag="WNA25_000267"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFA9823118.1"
                     /translation="MTAIYNTLTRQKEPFAPIDPENVRMYVCGMTVYDYCHLGHARVM
                     VVFDMIARWLRECGYPLTYVRNITDIDDKIIARAAENGETIGELNARFIQAMHEDADA
                     LGVLRPDIEPKATENIPQMIAMIETLIQNGKAYPAANGDVYYAVREFAAYGQLSGKSL
                     DDLRAGERVEVDGFKRDPLDFVLWKAAKAGEPAWESPWGNGRPGWHIECSAMSENLFG
                     DTFDIHGGGADLQFPHHENEIAQSVGASGHTCGHDHAQTHHGQSIASHVKYWLHNGFI
                     RVDGEKMSKSLGNFFTIREVLKQYDPEVVRFFILRAHYRSPLNYSDAHLDDAKGALTR
                     LYTTLKNTPAAEFDLSENANGYTRRFYAAMNDDFGTVEAVAVLFELAGEVNKTNDAHL
                     AGCLKALGGIIGLLQRNPIEFLQGGAVSDGLSNEEIEDLIARRKQARADKNWAESDRI
                     RDLLNEHKIILEDSAGGTTWRRG"
     gene            12623..13402
                     /gene="xth"
                     /locus_tag="WNA25_000268"
     CDS             12623..13402
                     /gene="xth"
                     /locus_tag="WNA25_000268"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFA9823119.1"
                     /translation="MLKIISANVNGIRSAYKKGFYEYIAASGADIVCVQELKAQEADL
                     SADMKNPHGMHGYWHCAEKRGYSGVAVYSKRKPDNVQIGMGIEEFDREGRFVRCDFGR
                     LGVISLYLPSGSSAEERQQVKYRFLDAFYPMLEAMKNEGRDIVVCGDWNIAHQNIDLK
                     NWKGNQKNSGFLPEEREWIGKVMHTLGWTDMWRTLYPDVPGYTWWSNRGQAYAKDVGW
                     RIDYQMVTPELAAKAVSAHVYKDEKFSDHAPLVVEYDYAAE"
     gene            13389..13733
                     /locus_tag="WNA25_000269"
     CDS             13389..13733
                     /locus_tag="WNA25_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823120.1"
                     /translation="MLPNKVLGKYDWNVDGKTGIGAAWVVAAFILPILVWAVFMLSRM
                     QGWLAPTKANPIWALVWLLICLPCLLIAAKCLGWKGWRRVVNIFVCLTVCAILSVPAS
                     LLIAFTLRDLLK"
     gene            13743..14273
                     /locus_tag="WNA25_000270"
     CDS             13743..14273
                     /locus_tag="WNA25_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFA9823121.1"
                     /translation="MESFELEDLTLWLIRDADEAEMWIDRWAVSYPVVQMSEASANGS
                     TEAWQSALQTAFERIQGRHIAVVAHGAGAAAFLAWLYRADILTQKKLAGIILVSPRPD
                     IFPDDAEHTFQRVRCPCRAALVVSEHGGVPHGWAQKQADLWNARLLVSPHSGSLNGML
                     GGWQWGMKLMQEMLLA"
     gene            complement(14366..15481)
                     /gene="asd"
                     /locus_tag="WNA25_000271"
     CDS             complement(14366..15481)
                     /gene="asd"
                     /locus_tag="WNA25_000271"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFA9823122.1"
                     /translation="MKVGFVGWRGMVGSVLMQRMKEENDFAHIPEAFFFTSSNVGGAA
                     PDFGQAAKTLLDANDVAELAKMDIIVTCQGGDYTKSVFQALRDSGWNGYWIDAASSLR
                     MKDDAIIALDPVNRNVIDNGLKNGVKNYIGGNCTVSLMLMALGGLFQNDLVEWATSMT
                     YQAASGAGAKNMRELISGMGAIHAQVADELADPSSAILDIDRKVSDFLRSEDYPKANF
                     GVPLAGSLIPWIDVDLGNGQSKEEWKGGVETNKILGRSGNPTVIDGLCVRIGAMRCHS
                     QAITLKLKKDLPVSEIEAILAGANDWVKVVPNEKEASIRELTPAKVTGTLSVPVGRIR
                     KLGMGGEYISAFTVGDQLLWGAAEPMRRVLRIVLGSL"
     gene            15656..16237
                     /locus_tag="WNA25_000272"
     CDS             15656..16237
                     /locus_tag="WNA25_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFA9823123.1"
                     /translation="MEEKNDYTDAVSDNRNGQEIEVGIAGAGDRILAALLNQLFTFLI
                     LLVPFVGLIAFAVKNEGRIGGREEIFGLLLGMTSFWVGLAGILAYTVIQIYYMSRDGQ
                     SLGKKIMRIRVLKTDGRNPGFVGTVLVREIAWSVLVAIIAAVIGLAVGENGENAINLL
                     AFLANFVLLFMVKRDRRTLYDILADTVVVKLPK"
     gene            complement(16299..17153)
                     /gene="folD"
                     /locus_tag="WNA25_000273"
     CDS             complement(16299..17153)
                     /gene="folD"
                     /locus_tag="WNA25_000273"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFA9823124.1"
                     /translation="MSAQLINGKEVSQKHLQAIAEAVAQRQQDNLHTPCLAVVLVGGD
                     PAGAVYVRNKKTACQKCGIKSLSYELPESTSQEELLALVDRLNADSEVDGILVQLPLP
                     KHLDSQAILERISPDKDVDGFHPYNVGRLAVKMPLMRPCTPKGVMTLLEAYGIDPKGK
                     KAVVVGASNIVGRPQALELLLARATVTVCHSATENLADEVAAADILVVGVGIPNFVKG
                     GWIKPGAVVIDVGINRLDDGSLCGDVEFETAKERAAMITPVPGGVGPMTIATLMENTL
                     HAASLHDA"
     gene            17376..17453
                     /locus_tag="WNA25_000274"
     tRNA            17376..17453
                     /locus_tag="WNA25_000274"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17411..17413,aa:Pro,seq:tgg)
     gene            17478..17554
                     /locus_tag="WNA25_000275"
     tRNA            17478..17554
                     /locus_tag="WNA25_000275"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17512..17514,aa:Arg,seq:tct)
     gene            17582..17657
                     /locus_tag="WNA25_000276"
     tRNA            17582..17657
                     /locus_tag="WNA25_000276"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17615..17617,aa:His,seq:gtg)
     gene            17869..18375
                     /gene="rfaE2"
                     /locus_tag="WNA25_000277"
     CDS             17869..18375
                     /gene="rfaE2"
                     /locus_tag="WNA25_000277"
                     /inference="COORDINATES: protein motif:HMM:NF013621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HFA9823125.1"
                     /translation="MVDAWSVPDFESKICPPEALAARLALLPRPLVFTNGCFDILHRG
                     HVTYLAQARSAGAALVLALNTDASVRRLGKGGDRPVNSLENRAAVAAALESVDLVTWF
                     DEDTPAALIEAVKPEVLVKGGDWVVDKIVGAAETLARGGQVFSIPFLHQTSTTKTLAK
                     IRAAEGGK"
     gene            18372..20150
                     /locus_tag="WNA25_000278"
     CDS             18372..20150
                     /locus_tag="WNA25_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/type III pantothenate kinase"
                     /protein_id="HFA9823126.1"
                     /translation="MTVLKPSHWRVLAELADGLPQHVSQLAREADMKPQQLNGFWQQM
                     PAHIRGLLRQHDGYWRLVRPLAVFDAEGLRDLGERSGFQTALKHECASSNDEILELAR
                     IAPDKAHKTICVTHLQSKGRGRQGRKWSHRLGECLMFSFGWAFDRPQYELGSLSPVAA
                     LACRRALGCLGLETQIKWPNDLVVGRDKLGGILIETVRAGGKTVAVVGIGINFVLPKE
                     VENAASVQSLFQTASRRGNADAAVLLETLLAELGAVLEQYAEEGFAPFLNEYETANRD
                     HGKAVLLLRDGETVCEGTVKGVDGRGVLHLETAEGEQTVVSGEISLRPDDRPVSVPKR
                     RDSERFLLLDGGNSRLKWAWVENGTFATVGSAPYRDLSPLGAEWAEKADGNVRIVGCA
                     VCGESKKAQVKEQLARKIEWLPSSAQALGIRNHYRHPEEHGSDRWFNALGSRRFSRNA
                     CVVVSCGTAVTVDALTDDGHYLGGTIMPGFHLMKESLAVRTANLNRPAGKRYPFPTTT
                     GNAVASGMMDAVCGSIMMMHGRLKEKNGAGKPVDVIITGGGAAKVAEALPPAFLAENT
                     VRVADNLVIHGLLNLIAAEGGESEHA"
     gene            20263..21087
                     /locus_tag="WNA25_000279"
     CDS             20263..21087
                     /locus_tag="WNA25_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HFA9823127.1"
                     /translation="MKWLFTLLAVLNIAVFGGTVGYKLAVKAAGGVPENRAVENTPPA
                     TPAAGNAAASVEDTAALLKPGDILSEEQAEQLRLKKEAEQKKLREKKQREEKARREKL
                     AAEKAQAERENGAADALCAAQASLTMDEDDYHRIKGLLGKWSHVASRSVEKRTAKAKP
                     ADKTYRVVLPVSADAENQAAELSAKGFNPIPFDGALSLGVGNSRENAQALQNRLADAG
                     FGGAHIVEHFAEADRQDDSLSVSRMTVLFTGVNAADADEIRKITSLYGKLNLKSCK"
     gene            complement(21593..21841)
                     /locus_tag="WNA25_000280"
     CDS             complement(21593..21841)
                     /locus_tag="WNA25_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HFA9823128.1"
                     /translation="MAFGGILKKAAKMPSEGFRRHRGPKRANRHSPASRLCREEQGNR
                     PENLAARPDTATNETPGLPGAQAAAQALSTAIEGCFVV"
     gene            complement(21820..22608)
                     /locus_tag="WNA25_000281"
     CDS             complement(21820..22608)
                     /locus_tag="WNA25_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFA9823129.1"
                     /translation="MLTLYGETFPSRLLLGTAAYPTPEILKQSVRTARPAMITVSLRR
                     TGCGGEAHGQGFWSLLQETGVPVLPNTAGCQSVQEAVTTAQMAREVFETDWIKLELIG
                     DDDTLQPDVFQLVEAAEILIKDGFKVLPYCTEDLIACRRLLDAGCQALMPWAAPIGTG
                     LGAVHAYALKILRERLPDTPLIIDAGLGLPSQAAQVMEWGFDGVLLNTAVSRSGDPVN
                     MARAFALAVESGRLAFEAGPVEARTKAQASTPTVGQPFWHSAEY"
     gene            complement(22687..22881)
                     /gene="thiS"
                     /locus_tag="WNA25_000282"
     CDS             complement(22687..22881)
                     /gene="thiS"
                     /locus_tag="WNA25_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFA9823130.1"
                     /translation="MNIILNGGPAELHGTSVADLIAQTAPQKPFAVAVNTVFIPKGAY
                     TETVLHENDKIDIVRPVVGG"
     gene            complement(23303..23920)
                     /gene="thiE"
                     /locus_tag="WNA25_000283"
     CDS             complement(23303..23920)
                     /gene="thiE"
                     /locus_tag="WNA25_000283"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFA9823131.1"
                     /translation="MTFPPLKSLLKFYAVVPTADWVGRMVKAGADTVQLRCKTLHGNE
                     LKREIARCVAACQGSRTQLFINDHWREAIEAGAYGVHLGQEDMDTADLAAIAAAGLRL
                     GLSTHSVAELDRALSVHPGYIASGAIFQTTTKQMPTAPQGLDKLREYVEQARGTPVVA
                     IGGIDLNNARAVLATGVSSLAAVRAVTEAANPEAVVKAFQALWDG"
     gene            complement(23942..25042)
                     /locus_tag="WNA25_000284"
     CDS             complement(23942..25042)
                     /locus_tag="WNA25_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFA9823132.1"
                     /translation="MTRIAVLGGGLSGRLTALQLAEQGYQIELFDKGTRQGEHAAAYV
                     AAAMLAPAAEAVEATPEVIRLGRQSIPLWRGIRCRLNTHTMMQENGSLIVWHGQDKPL
                     SSEFVRHLKRGGVADDEIVRWRADEIAEREPQLGGRFSDGIYLPTEGQLDGRQILSAL
                     ADALDELNVPCHWEHECAPQDLQAQYDWVIDCRGYGAKTAWNQSPEHTSTLRGIRGEV
                     ARVYTPEITLNRPVRLLHPRYPLYIAPKENHVFVIGATQIESESQAPASVRSGLELLS
                     ALYAVHPAFGEADILEIAAGLRPTLNHHNPEIRYSRERRLIEINGLFRHGFMISPAVT
                     AAAVRLAVALFDGKDAPERDEESGLAYIGRQD"
     gene            complement(25039..26265)
                     /gene="cytX"
                     /locus_tag="WNA25_000285"
     CDS             complement(25039..26265)
                     /gene="cytX"
                     /locus_tag="WNA25_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HFA9823133.1"
                     /translation="MSGNASSPSSSAAIGLIWFGAAVSIAEISTGTLLAPLGWQRGLA
                     ALLLGHAVGGALFFAAAYIGALTGRSSMESVRLSFGKCGSVLFSVANMLQLAGWTAVM
                     IYVGATVSSALGKVLWDGESFVWWALANGALIVLWLVFGARRTGGLKTVSMLLMLLAV
                     LWLSVEVFASSGTNAAPAVSDGMTFGTAVELSAVMPLSWLPLAADYTRQARRPFAATL
                     TATLAYTLTGCWMYALGLAAALFTGETDVAKILLGAGLGITGILAVVLSTVTTTFLDT
                     YSAGASANNISARFAETPVAVGVTLIGTVLAVMLPVTEYENFLLLIGSVFAPMAAVLI
                     ADFFVLKRREEIEGFDFAGLVLWLAGFILYRFLLSSGWESSIGLTAPVMSAVAIATVS
                     VRLFFKKTQSLQRNPS"
     regulatory      complement(26331..26435)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            26510..27994
                     /gene="tldD"
                     /locus_tag="WNA25_000286"
     CDS             26510..27994
                     /gene="tldD"
                     /locus_tag="WNA25_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFA9823134.1"
                     /translation="MPYNQAPDFLCADTMHPTYSAVQARLLEANRLCPELLAKSLCII
                     GAHHVDYADIYCQRTAYESWHLEEGIVKSGSFQIDQGVGVRAVSGDKTAFAYADSLCI
                     DSINRSARAVRAIGAAGGKVSAKMPSETRGKPVCSASDPIAGLDSAAKVALLNKVEAI
                     AKAADPRIVQVMAGLTCEYDMVYLARLDGKHAADIRPMVRLNVTVIAKQGERREQGGA
                     GGGGRYDLAYFDETLVRQFVDAAVKQALTNLESRPAPAGEMTVVLGNGWPGVLLHEAV
                     GHGLEGDFNRKGTSVFSGRIGERVAAKGVTVVDQGDIAGRRGSLNIDDEGNETRRTVL
                     IEDGILVGYMQDETNARLTGTQSTGNGRRQSYASVPMPRMTNTFMENGSYEPEEIIAS
                     IDKGIYAVNFGGGQVDITSGKFVFGASEAWWVEGGRLQYPVKGATIIGNGPEVLKHVS
                     MIGNDTALDSGVGVCGKEGQSVPVGVGQPTLRIDAGLTVGGSAI"
     gene            28138..28797
                     /locus_tag="WNA25_000287"
     CDS             28138..28797
                     /locus_tag="WNA25_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFA9823135.1"
                     /translation="MNWPYLIDAVPKFADAAKLTLELSVYGVVLSLLFDLPVAVVTAY
                     RIRPFYALARAYIELSRNTPLLIQLFFLYYGLPKMGIKWDGFTCGVIALVFLGASYMA
                     EAVRAGILAVPKGQVGAGKAIGLSRFQVFRYVELPQVWAVAVPAIGANILFLMKETSV
                     VSTVGIAELLFVTKDVIGMDYKTNEALFLLFAAYLIILLPVSLLARRIENRVRSAKYG
                     V"
     gene            28787..29455
                     /locus_tag="WNA25_000288"
     CDS             28787..29455
                     /locus_tag="WNA25_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFA9823136.1"
                     /translation="MAFEWLFEGKNAARLGEGLLLTAQISLISVAASCVLGTLFGLVL
                     RSRNRLVRFVGRFYLETIRIVPILVWLFGLYFGLSVWTGIHIGGFWVCVWVFSLWGVA
                     EMGDLVRGALESIEKHQVESGLAPGLSRGQVFRCIELPQSIRRVLPGAVNLFTRMIKT
                     SSLAWLIGVIEVVKVGQQIIENSLLTQPNASFWVYGLIFMLYFFCCWPLSLLAAKLEQ
                     KWEH"
     gene            29457..30188
                     /locus_tag="WNA25_000289"
     CDS             29457..30188
                     /locus_tag="WNA25_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFA9823137.1"
                     /translation="MALLSIRKLHKQYGSVTAIQSLDLDLEKGEVIVLLGPSGCGKST
                     LLRCVNGLEPHQGGSIVMDGVGEFGKDVSWQTARQKVGMVFQSYELFAHMTVIENILL
                     GPVKVQNRDRAEAEAQAGKLLERVGLLDRKNAYPRELSGGQKQRIAIVRALCLNPEVI
                     LLDEITAALDPEMVREVLEVVLELAREGMSMLIVTHEMGFARKVADRIVFMDKGGIVE
                     SSDPETFFSAPKSERARQFLAGMDY"
     gene            30301..31155
                     /locus_tag="WNA25_000290"
     CDS             30301..31155
                     /locus_tag="WNA25_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFA9823138.1"
                     /translation="MKLNAKLKALLASAAIAVGLTACGGGSGDAQSSQSSGAATVAAI
                     KEKGVIRIGVFGDKPPFGYVDANGKNQGFDVEIAKDLAKDLLGSPDKVEFVLTEAANR
                     VEYVRSGKVDLILANFTQTPERAEAVDFADPYMKVALGVVSPKNKPITDMAQLKDQTL
                     LVNKGTTADAFFTKSHPEVKLLKFDQNTETFDALKDGRGVALAHDNALLWAWAKENPN
                     FEVAIGNLGPAEFIAPAVQKGNADLLNWVNGEIAAMKKDGRLKAAYEKTLLPVYGEKV
                     KPEALLAE"
     gene            31572..32393
                     /gene="prmC"
                     /locus_tag="WNA25_000291"
     CDS             31572..32393
                     /gene="prmC"
                     /locus_tag="WNA25_000291"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFA9823139.1"
                     /translation="MTFDEWLGLSKLPKIEARMLLQYVSEYTRVQLLTRGGEEMPDEI
                     RQRADRLAQRRLNGEPVAYILGVREFYGRRFTVNPNVLIPRPETEHLVEAVLARLPEN
                     GRVWDLGTGSGAVAVTVALERPDAFVRASDISTPALETARKNAADLGARVEFAHGSWF
                     DTDMPSERQWDIIVSNPPYIENGDKHLSQGDLRFEPQIALTDFSDGLSCIRTLAQGAP
                     DRLAEGGFLLLEHGFDQGAAVRGVLAENGFSGVEILPDLAGLDRVTLGKYMKHLK"
     gene            32479..33867
                     /locus_tag="WNA25_000292"
     CDS             32479..33867
                     /locus_tag="WNA25_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HFA9823140.1"
                     /translation="MNAVVVAVIVMLVLSLSRVHVVLSLTVGAFVGGAVAGMPLQNIA
                     DAAGQVSQAGIIPVFNKGLEGGAKIALSYAMLGAFAMAITHSGLPQQLAGAVVRKLNR
                     GGMPDSVRSGEGAVKWLLLSIILVMGIMSQNVIPIHIAFIPMIVPPLLLVFNRLKIDR
                     RLIACVITFGLVTTYMFLPYGFGAIFLNEILLGNIHSAAPQLDVKNINVMAAMAIPAL
                     GMLAGLLLAFVHYRKPRLYQSNNADTAGNADAANRPQPSAYRSLVAAAAIAVCFAIQL
                     MYEDSLVLGAMLGFAVFMMLGVINRDKANDVFGEGIKMMAMVGFIMIAAQGFAAVMNA
                     TGHIQPLVESSMAIFGNSKGMAALAMLVVGLLVTMGIGSSFSTLPIIAAIYVPLCTGL
                     GFSPLATAAIVGTAGALGDAGSPASDSTLGPTMGLNADGRHDHIRDSVIPTFIHYNIP
                     LLIAGWIAAMVL"
     gene            33868..34092
                     /locus_tag="WNA25_000293"
     CDS             33868..34092
                     /locus_tag="WNA25_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFA9823141.1"
                     /translation="MDAVQELERRIVELEIQTALQEDVISGLNAMVAELRQTLDLQQA
                     QLRLLYQKMQDRNPDAQEPYSLRDEIPPHY"
     gene            complement(34492..35385)
                     /locus_tag="WNA25_000294"
     CDS             complement(34492..35385)
                     /locus_tag="WNA25_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HFA9823142.1"
                     /translation="MPHPCQTVHTLTTHVPKCRLKLNAARRRRYNRPQLFFPNIIMTA
                     TEHDNDDALLLRYSRHILLDEIGIEGQQKLSAAHILVVGCGGLGAAALPYLAASGVGT
                     LTIADSDTVELHNLQRQVAFDEGDVGKPKAETLAGRLKRINHTVDVRAVNEKLDGCRL
                     TGLVQTADIVLDCCDNYATRQAVNRACVQTKTPLVSGAAVRFEGQLAAYRPDLPDSPC
                     YACLFDGGSASDGICSLFGVLSPLVGIVGCTQAAEALKILLEAGEPSHGRLAVYRALE
                     GGWQYFDLPRNPECPVCGAAR"
     gene            35428..38130
                     /gene="ppc"
                     /locus_tag="WNA25_000295"
     CDS             35428..38130
                     /gene="ppc"
                     /locus_tag="WNA25_000295"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFA9823143.1"
                     /translation="MQLHILNNPKDAALAADAEFLKQSLFNLLHEEASPLVVETVKLL
                     STSDDSAALIEKVLPQLDERQTYDLTLACGLFAQILNIAEDVHHERRRQIHEDAGHNA
                     AEGSLTETVRRLKAGKADGKSVQRQLDNTSVTAVLTAHPTEVQRQTVLNFNRRIRALL
                     PQRERCTNADALARLRREIDTVLLGLWQTSETRRHKLSVNDEINNGVSIFPMSFFEAL
                     PKLYRKMEHDFQTAYPDVRVPNILKIGGWIGGDRDGNPFVSGETLRFAFRRHADAVFR
                     FYRSELDKLYRELPLSIRRVKVNDDVMALAALSPDEEIARTEEPYRRAIAYIMARAMG
                     KARSLGLGMGCKFGFLEPYASAQKFLDDLKKLQRSLIDNGSRLLAEGRLADLIRSVSV
                     FGFHMMPLDLRQHAGKHADVVAELFQHAGLEDYNSLNEEQKQAALLRELGHQRPLYSP
                     FITYSDHTRRELAIFNEARKIKDEFGEDAVTQSIISNCEQPGDLLALALLLKESGLLA
                     VENGKPHSRINIVPLFETIEALENACPVMETMFRLDWYDALLESRGNIQEIMLGYSDS
                     NKDGGYVTSSWCLHQAELGLVELFKKYDVRMRLFHGRGGSVGRGGGPSYQAILAQPAG
                     SVAGQIRITEQGEVITAKYADPGNAQRNLETLVAATLEASILPDKKDPDAKLMQALSD
                     VSFKYYRELITHPDFIDYFLQTSPIQEIATLNLGSRPASRKTLARIQDLRAIPWVFSW
                     MQNRLMLPAWYGFGSAVETLCEGSPETLAALRGHAQNNPFFQAMLSNMEQVMAKTDIT
                     LAENYAGLSESPEKAKVIFGMIKEEYRRSRKALLDLLQTEELLRDNRSLARSLALRIP
                     YLNALNGLQVAMLKRLRKEPDNPHALLMVHLTINGVAQGLRNTG"
     gene            38253..39242
                     /locus_tag="WNA25_000296"
     CDS             38253..39242
                     /locus_tag="WNA25_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFA9823144.1"
                     /translation="MKITVIGAGSWGTALALHFSQHGNRVSLWTRNADQVRQMQEARE
                     NKRGLPGFSFPETLEVCADLAEALKDSGLVLIVTSVAGLRSSAELLKQYGAGHLPVLA
                     ACKGFEQDTGLLTFQVLKEVLPDNKKIGVLSGPSFAQELAKQLPCAVVLASENQEWIE
                     ELVPQLNTTVMRLYGSTDVIGVAVGGSVKNVMAIATGLSDGLEYGLNARAALVTRGLA
                     EITRLASAMGAQPKTMMGLAGIGDLILTCTGALSRNRRVGLGLAEGKELHQVLVEIGH
                     VSEGVSTIEEVFNTACKYQIDMPITQTLLQLIRKEMTPQQVVERLMERSARFE"
     gene            39296..39625
                     /locus_tag="WNA25_000297"
     CDS             39296..39625
                     /locus_tag="WNA25_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823145.1"
                     /translation="MKQNIEKLESSVYTLVQKFETLVSENRRLKETVAELERAHERQK
                     LEHETAVDELSEALLVQVGKLKEDLQNKIDSLTEENARYRALLEQSREKISALAARLP
                     QRQETQQ"
     gene            39636..39941
                     /locus_tag="WNA25_000298"
     CDS             39636..39941
                     /locus_tag="WNA25_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFA9823146.1"
                     /translation="MNIEQVYIEVMHARLTVNTPAEEKDTLLQAVGMLNGKAEAIREG
                     GRVADSEKIVIMAALNVVHDLLKTSLNGGDLAIGDFARKITDMDNACQKALSRLGQE"
     gene            39947..40126
                     /gene="ssrS"
                     /locus_tag="WNA25_000299"
     ncRNA           39947..40126
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="WNA25_000299"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            40304..40735
                     /gene="rplM"
                     /locus_tag="WNA25_000300"
     CDS             40304..40735
                     /gene="rplM"
                     /locus_tag="WNA25_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFA9823147.1"
                     /translation="MKTFSAKPHEVKREWFVIDAQDKVLGRVATEVASRLRGKHKPEY
                     TPHVDTGDYIIVINADKLRVTGAKFEDKKYFRHSGFPGGIYERTFREMQDQFPDRALE
                     QAVKGMLPKGPLGYAMIKKLKVYAGAEHAHAAQQPKVLELK"
     gene            40748..41140
                     /gene="rpsI"
                     /locus_tag="WNA25_000301"
     CDS             40748..41140
                     /gene="rpsI"
                     /locus_tag="WNA25_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFA9823148.1"
                     /translation="MNGKYYYGTGRRKSSVARVFLTKGTGQIIVNGRPVDEFFARETS
                     RMVVRQPLVLTENAESLDIKVNVVGGGETGQSGAIRHGITRALIDFDAALKPALSQAG
                     FVTRDAREVERKKPGLRKARRAKQFSKR"
     gene            complement(41521..42015)
                     /locus_tag="WNA25_000302"
     CDS             complement(41521..42015)
                     /locus_tag="WNA25_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823149.1"
                     /translation="MRLTKYPYNSIRELEDYLINTYQKYIILQEGGKEIYRCFILSFY
                     KEFNIGIGLAVSCISIPPKVLMLDDKNIFIGFDSVVFCISIQNSKVNILNIDGIVFDI
                     YLLDNQKICIIHELGAIITDKNLIRENSVSTDIISDWEIDKVNKLIILKGLDSEKIIS
                     LNYD"
     gene            complement(42208..43137)
                     /locus_tag="WNA25_000303"
     CDS             complement(42208..43137)
                     /locus_tag="WNA25_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFA9823150.1"
                     /translation="MDSIIELRHLKTLLALEETGSVSLAAKRVFLTQSALSHQIRMLE
                     NHYGTPLFERKSTPLRFTPVGERLLRLAHELIPQVAVAERDLARITEGEAGELRIAVE
                     CHTCFDWLMPAMGEFRPMWPQVELDIVSGFQADPVGLLLQHRADLAIVSEAEKQSSIS
                     FHPLFAYEMVGICAPDHPLAAKNVWTAEDFIGETLITYPVPDEMLDLPKKILIPKNIN
                     PPRRHSELTIAIIQLVASRRGIAALPYWTVMPYLEKGYVVHRQITADGLQSKLYAAIR
                     TEDTDKSYLNNFCQIIRERGFADLPGLSELEPV"
     gene            43266..44015
                     /locus_tag="WNA25_000304"
     CDS             43266..44015
                     /locus_tag="WNA25_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFA9823151.1"
                     /translation="MVLRRDFLAWCDETLQTASFKDYAPNGLQVEGREYIGKIVTSVT
                     ASRAAIDFAVEQKADLLLVHHGMFWKNELPTVTGWKKERIAALLRHDINMAGYHLPLD
                     VHPILGNNAQLADRLGFATEKRFGEQNLLNSGSLKQAKTLGALAAHIETVLQRKPVAI
                     GNPEREIRRVAWCTGGAQGFFQTAIDEGVDLYLTGEISEAQYHLANETGTAFISAGHH
                     ATERYGVRALAESAAEVFGLEVCHFDENNPA"
     gene            44138..44719
                     /gene="petA"
                     /locus_tag="WNA25_000305"
     CDS             44138..44719
                     /gene="petA"
                     /locus_tag="WNA25_000305"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HFA9823152.1"
                     /translation="MDNQEINNGRRRFLTLATCGAGGVAALGVATPFVASFFPSEKAK
                     AAGAAVEVDVSKIEAGQLLTAEWQGKPIWVLNRTDQQLKDLKGLNGELTDPNSDAEQQ
                     PEYAKNETRSIKPNILVAIGICTHLGCSPTFRPDIAPADLGADWKGGFFCPCHGSKFD
                     LAGRVYKGVPAPTNLVVPPYKYLSDTTILVGED"
     gene            44738..46087
                     /locus_tag="WNA25_000306"
     CDS             44738..46087
                     /locus_tag="WNA25_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HFA9823153.1"
                     /translation="MANQTNSKAKALLGWMDARFPLSKMWNEHLAQYYAPKNFNFWYY
                     FGSLALLVLVIQIVSGIFLTMNYKPDGNLNAYHLPAAFTAVEYIMRDVSGGWIIRYMH
                     STGASFFFIVVYLHMFRGLIYGSYKKPRELVWIFGSLIFLALMAEAFMGYLLPWGQMS
                     FWGAQVIINLFSAIPVIGPDLSTWIRGDFNVSDVTLNRFFALHVIAVPLVLLGLVVAH
                     IIALHEVGSNNPDGVEIKKNKDENGIPRDGIPFHPYYTVKDILGVVVFLIVFCAVLFF
                     APEGGGYFLEAPNFDAANALKTPPHIAPVWYFTPFYAILRAIPSFLGTQVWGVIGMGA
                     AVVLIALLPWLDRGEVKSVRYRGPIFKTALVLFIISFIGLGILGAMVATDTRTLVARI
                     LSFVYFAFFLGMPFYTKLDKNKPVPERVTMSTAKQKIMFFVYVGITAVGAYLFATNI"
     gene            46102..46890
                     /locus_tag="WNA25_000307"
     CDS             46102..46890
                     /locus_tag="WNA25_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HFA9823154.1"
                     /translation="MKQAMKNWFAALLSAVPMGAAFASGGHAHYEKVDIDLRDQVSLQ
                     RGAQIFTNYCLSCHSASGMRFNRLKDIGLTDEEIKKNLMFTTDNVGDVMHSAMNPKDA
                     AKWFGAAPPDLTLIARSKGADYLYAYMRGFYKDPTRPSGWNNTVFDKVGMPHPLWEQQ
                     GVQAVELDAKGQPVMVKDEHGEMKPKLYWESTGLHSRRLPNGKVIQKEYDAYVRDLVN
                     YLVYMGEPAQLQRKRIGYVVMIFLFAVMLPLAYFLKKEYWKDVH"
     gene            47222..49012
                     /gene="rmuC"
                     /locus_tag="WNA25_000308"
     CDS             47222..49012
                     /gene="rmuC"
                     /locus_tag="WNA25_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFA9823155.1"
                     /translation="MELMTVLLPLAALVSGVLFTWLLMKGRFQGEFAGLNAQLAEKAA
                     RCDFIEQAHAEIASELAALDGKYEHLQDENYALGNRFSAAEKQIAHLQEKEAESVRLK
                     QSYIDLQEKAQGLAVENERLATQLGQERKAFAEQYALERQIRQRVETDLEESRQTVRD
                     VQNDLSDVGNRFAAAEKQIAYLQEKEAEAERLRQSHTELQEKAQGLAVENERLATQIE
                     QERLASEEKLSLLGEARKSLSDQFQNLANTILEEKSRRFTEQNREQLHQVLNPLNERI
                     HGFGELVRQTYDKESRERLTLENELKRLQGLNAQLHSEAKALTNALTGTQNKVQGNWG
                     EMILETVLENSGLQKGREYVVQAASVRKEEDGGTRRLQPDVLVNLPDNKQIVIDSKVS
                     LTAYVRYTQAADADEAARELAAYIASIRAHMKGLSLKDYTDLEGVNTLDFVFMFIPVE
                     PAYLLALQNDAGLFQECFDKRIMLVGPSTLLATLRTVANIWRNEQQNQNALAIADEGG
                     KLYDKFVGFVQTLESVGKGIDQAQNSFQTAFKQLAEGRGNLVGRAEKLRLLGVKAGKQ
                     LQRDLVERANEQTAFALGKPSEQEAADEAE"
     gene            complement(49079..49711)
                     /locus_tag="WNA25_000309"
     CDS             complement(49079..49711)
                     /locus_tag="WNA25_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFA9823156.1"
                     /translation="MTLRYEILPVTPFRQNCTLIWDDESGEAVLTDVGGDVPFLLQAL
                     ANRKLTLTAIWLTHGHLDHAGGVVEMLETHKVPVLGPHREDEFLLQSLPQTTAQYGFP
                     VSPAFAPNRWLEEGETLTVGRYAFQVLHIPGHTPGHVVFYCAEAELLIAGDVLFYETI
                     GRTDFPRGNHADLINNIRNKLFAFPETVQVVAGHGRMTSIGHEKRHNPFF"
     gene            49804..50628
                     /locus_tag="WNA25_000310"
     CDS             49804..50628
                     /locus_tag="WNA25_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HFA9823157.1"
                     /translation="MIKKIIGGIIPIFTAVFIPASAGAADLMLAQEYKGQDIAGWAMS
                     EKLDGVRAYWDGKHLISRQGYAFTPPKGFTAQFPPYPLDGELYSGRGQFEQISATVRS
                     VSSDWRGIRLHVFDVPKAQGNLYQRLAVATQWLKTHPNAPITIIPQIKVRDRRHAMDF
                     LKQIEAQGGEGVMLRQPESRYSGGRSSQLLKLKSQYDDECTVTRHYEGKGRNAGRLGA
                     VGCKNRHGEFRIGSGFKDKDRDNLPKIGTLITYRYRGFTRKGTPKFATFVRVRTDR"
     gene            50697..51260
                     /locus_tag="WNA25_000311"
     CDS             50697..51260
                     /locus_tag="WNA25_000311"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HFA9823158.1"
                     /translation="MTDLETKRLETQAMLENADLLFDQGQCRAALQKVAYEITRDLGD
                     KYPLLLPVMGGAVVFTGQLLPLLRFPLDFDYVHVSRYGDKLEGGAFNWKRMPDAEQIR
                     GRHVVVLDDILDEGHTMSAIQAKLLEMGAASCRAAVFANKLIDKPKPIRADYVGLDVP
                     NRYVFGYGMDAAGCWRNLGEIYALDGK"
     gene            51343..51780
                     /locus_tag="WNA25_000312"
     CDS             51343..51780
                     /locus_tag="WNA25_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFA9823159.1"
                     /translation="MIGLLIITHETIGEAYRKLAHHFFPGGLPENVRILGVQPTEGQD
                     DIINNAISALQEFPENHGVLIMTDIFGATPCNAARRLVRENKSAILTGLNAPMMIKAV
                     QYSPAAEDFAAFTECVREAAVKGIFAITSAPEDLVCRRSGDAV"
     gene            52001..52270
                     /locus_tag="WNA25_000313"
     CDS             52001..52270
                     /locus_tag="WNA25_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFA9823160.1"
                     /translation="MLKQSIEIINKLGLHARASSKFTQTASQFKSEVWVTKNGSRVNG
                     KSIMGLMMLAAAKGTVIELETDGLDEAAAMKALTDLINDYFGEGE"
     gene            52270..54045
                     /gene="ptsP"
                     /locus_tag="WNA25_000314"
     CDS             52270..54045
                     /gene="ptsP"
                     /locus_tag="WNA25_000314"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFA9823161.1"
                     /translation="MSIVLHGVAAGKGIAVGCAHLIARGTEEVPQYDVAQADTDAEAE
                     RFDAAVKATRKELEQLRSAIPENAPTELGAFISLHLMLLTDVTLSREPVDILREQKIN
                     AEWALKQQSDKLAAQFDNMDDAYLRERKQDMLQVVRRIHNNLIGQGNELEVADNLFDE
                     TVLIANDLSPADTVLFKEQRIAAFVTDAGGPTGHTAILGRSLDIPSVVGLHNARKLIT
                     EGETVIVDGINGVLIISPDESVLNEYRRRAREYRSHKRDLNKLKKTAAATADGVCIEL
                     VGNIESAEDVKPLHNLGADGIGLFRSEFLYLNRDTMPSEDEQYEVYSAIVKKMKGKSV
                     TIRTVDLGVDKNPRWFGKNSTPNGSLNPALGMTGIRLCLAEPVMFRTQMRAILRAAVH
                     GPVRMMWPMITSVSEVRQCLIHLDTAQRQLAERGDAFGEVGIGCMIEIPSAALTVGSI
                     LKLVDFISVGTNDLIQYILSVDRGDDSVSHLYQPGHPSVLKMLQHVIRTANRMDKDVS
                     VCGEMAGDTAFTRVLLGMGLRRFSMNPNNILPVKNIILHSNAGQLEGDIAKVIRCEDE
                     EKSEKLIKQINSVSVAEEAEVKGRK"
     gene            complement(54172..55113)
                     /locus_tag="WNA25_000315"
     CDS             complement(54172..55113)
                     /locus_tag="WNA25_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFA9823162.1"
                     /translation="MLELNGLCKCFGGKTVADNICLTVGRGKILAVLGRSGCGKSTLL
                     NMIAGIVRPDGGEIRLNGENITCMPPEKRRISLMFQDYALFPHMSALENTAFGLKMQK
                     MPKAEAERLALSALAEVGLENEAHRKPEKLSGGEKQRLALARALVVRPSLLLLDESFS
                     SLDTHLRDRLRRMTAERIRKGGIPAVLVTHSPEEACTAADEIAVMHEGKILQCGTPET
                     LIQTPAGVQVARLMGLPNTDDDRHIPQNAVCLDNHGTECRLLSLVRLPDSLRLSAVHP
                     EHGELTLNLTVGQHTDGISGNGTVRIRVDEGRIVRFR"
     gene            complement(55122..55997)
                     /locus_tag="WNA25_000316"
     CDS             complement(55122..55997)
                     /locus_tag="WNA25_000316"
                     /inference="COORDINATES: protein motif:HMM:NF012514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFA9823163.1"
                     /translation="MPTVRFTESVSKQDLDALFERAKASYGAESCWKTLYLNRLPLGN
                     LSPEWAERIKKDWEAGCSESSDGIFLNADGWPDMGGRLQHLARTWNKAGLLHGWRNEC
                     FDLTDGGGNPLFTLERAAFRPFGLLSRAVHLNGLVESNGRWHFWIGRRSPHKAVDPGK
                     LDNIAGGGVSGGEMPSEAVCRESSEEAGLDKTLFPLIRPVSRLHSLRPVSRGVHNEIL
                     YVFDAVLPETFLPENQDGEVAGFEKMDIGGLLDAMLSKNMMHDAQLVTLDAFYRYGLI
                     DAAHPLSEWLDGIRL"
     gene            complement(56180..56256)
                     /locus_tag="WNA25_000317"
     tRNA            complement(56180..56256)
                     /locus_tag="WNA25_000317"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56220..56222),aa:Met,seq:cat)
     regulatory      56405..56504
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            56595..58496
                     /gene="thiC"
                     /locus_tag="WNA25_000318"
     CDS             56595..58496
                     /gene="thiC"
                     /locus_tag="WNA25_000318"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFA9823164.1"
                     /translation="MTTPKKTAKISGNEARELSDLSEDIGIRFKYPNSERVYLQGSRD
                     DIRVPLREIRQDDTYTAQGTEANPPIPVYDTSGAYGDPAAHIDLKQGLPHIRTAWLDE
                     RGDTEILPKLSSEYGIECAHDPKTAHLRFNQITRPRRAKAGRNVTQLHYARQGIITPE
                     MEFAAIRERMKLDELFRRPEYAKLLKQHTGQSFGANIPTRPDQITPEFVRQEIAAGRA
                     IIPANINHPELEPMIIGRNFRVKINGNLGNSAVTSSLTEEVEKMVWSLRWGADTIMDL
                     STGAHIHETREWIIRNAPVPIGTVPIYQTLEKTGGIAEDLTWDLFRDTLIEQAEQGVD
                     YFTIHAGVLLRYVPMTANRLTGIVSRGGSIMAKWCLAHHRENFLYTHFDEICEIMKAY
                     DVSFSLGDGLRPGCIADANDESQFAELHTLGELTDKAWKHDVQVMIEGPGHVPLQRVK
                     ENMTEELQHCFEAPFYTLGPLVTDIAPGYDHITSGIGAANIGWYGTAMLCYVTPKEHL
                     GLPDKEDVRTGIITYKLAAHAADLAKGWPGAQLRDNALSKARFEFRWRDQFRLSLDPE
                     RAESFHDETLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYADKQKAQRQGMEEKAVEF
                     VKKGAKIYS"
BASE COUNT        13503 a        15511 c        16522 g        13168 t
ORIGIN      
        1 ttgatatata tagataaata actattttta ttttttgttt cgcagttccc cggcttggcg
       61 ggcgacagaa aaactttgca tttcccagta aaatccacta cattcacgat aacatattac
      121 aagcccctga agcggtgtcg gaagccatac ttgcaacgca ttccaacccc gaaacagcgt
      181 gctcggacac acaccattgc cggtttcccc tgcttttgcg attcaggcgt ttgtagtacg
      241 gcaaataacc ataagggcag aagatgcctg ttttttagga gtaaaccatg gctgaagcga
      301 cagacgttgt cttggtgggc ggcggcatta tgagcgcgac tttgggcgtt ttgctcaaag
      361 aactcgaacc gtcttgggaa atcaccctga ttgaacgctt ggaagatgtg gcgttggaat
      421 cgtcaaacgc gtggaacaac gccggcacgg ggcattccgc gctgtgtgaa ttgaactatg
      481 cgccgttggg tgcaaatggg attatcgatc cggcgcgcgc cctcaatatt gccgaacagt
      541 ttcatgtcag ccgccagttt tgggcgacgc tggtcgcgga aggcaagttg gaagacaatt
      601 ccttcatcaa tgccgtgccg catatgtctt tggtgatgaa tgaagaccat tgttcttatc
      661 ttcaaaaacg ttatgacgcg tttaaaaccc aaaaactttt tgaaaatatg gaattttcca
      721 ccgatcggaa caaaatttcc gattgggctc cgctgatgat gcgcggccgg gacgaaaacc
      781 aacccgtcgc cgccaactac tccgccgaag gtacggatgt cgatttcgga cggctgacgc
      841 gccagatggt gaaatatttg cagggcaagg gcgtaaaaac cgagttcaac cgccacgtcg
      901 aagacatcaa acgcgaatcc gacggcgcgt gggtgctcaa aaccgccgat acccgcaacc
      961 ccgacgggca gctcaccctc cgtacccgct tcctcttcct cggcgcgggc ggcggcgcgc
     1021 tgaccctgct gcaaaaatcc ggcatccccg aaggcaaagg ctacggcggc ttccccgtgt
     1081 ccggcctgtt cttccgcaac agcaaccccg aaaccgccga acaacacaac gccaaagtgt
     1141 acgggcaggc ttccgtcggc gcgccgccga tgtccgtccc gcacctcgac acacgcaacg
     1201 tggacggcaa acgccacctt atgttcggcc cttacgcagg cttccgttcc aacttcctca
     1261 agcaaggctc gcttatggat ttgccgctgt ccatccatat ggacaatctc tatcctatgc
     1321 tgcgcgccgg ctgggcgaat atgccgctga ccaaatacct gctgggcgaa ttgcgtaaaa
     1381 ccaaagaaga acgcttcgcc tccctgctgg aatactaccc cgaggcaaac cccgacgact
     1441 gggaactcat caccgcaggg caacgcgtcc aaatcattaa aaaagactcc gaaaaaggcg
     1501 gcgtgctcca gtttggtacg gagattgtcg cccacgccga cggctcactc gccgcattgc
     1561 tgggcgcgtc gccgggcgca tcgaccgctg tgccgctgat gatccggctg atgcaccaat
     1621 gcttccccga gcgcgccccg tcttgggaag gtcgtctgaa agagctggta ccgggttacg
     1681 gcatcaagtt gaacgaaaac cctgaaaggg cggatgaaat tatcgcctat accgcgaaag
     1741 tgttggatat ttaagccgaa tctttcaaga aaagccgtac ccccatatgg tgttgatgcc
     1801 gtccctgccg gttatgccct tcatccggca gggcaggttt tgatacacgg ggcaggtaat
     1861 cgttttttcc cttttgcgaa taatggaacg gtcaaaaaac agcgttttca gaaatgaaaa
     1921 cgctgttttt tgttgcctga ttaacgtggg gaacagtctt tgaagacgat ttggttgtca
     1981 ggtgcggtaa tcataatagg ctgtttgcgg taggatttgc tgtccattgc gccggtgctc
     2041 agcacataac cgccttcttt gccgtagaac gtgtccatat tgtcggattt atccaaattg
     2101 atgggcattt gcacacgttt gccgttgatg acggcggagg cgtatgtggt cagaccctgt
     2161 ttgttgaagc cgtaggttac tttgactttt ttaccttgct ggcagacgta gctgacggtt
     2221 tttttggcaa cggtggggtt gttcgtgccg gcagcagcag ccatactgct gagcgcgatt
     2281 gcggaagaca ggattgcggt ggtcagaagt ttcattttta ttcctttgga tggatgtgga
     2341 gtttgcccgt ttcgagctgt ttgacattct aatgtcggcg tatcctgaaa taaacaggaa
     2401 attggataaa gtttgtaaaa aggcgggttc gggcggttcg gcggtttgag gcaggggcgg
     2461 caggcgggat acggcggcct gtccggtgtt tcagacggca ttgtttgcgt ttttcggaaa
     2521 tgaaaaacct tcgggcatcc ggaggttgcg gtttaacttt gtttttttct ttcggacttg
     2581 gtctgtttgg acaattcggc atacaggtcg ggatggtgtt ttttcaacag ggcggcgatt
     2641 tccatatctt ttttgttgac tttcgccaaa tcgatgcatt cgatatggac gaggcggagc
     2701 agttcgcgta cgggtttggg cattttgcgg ccggtttcgt agcgcgagcc tccagactgg
     2761 gtaacgccga ccttgcccca gaagtctatc tggttcagcc cggtcttttt acggatggct
     2821 ttaacatctt ctatattttc aaatattttc ataaaacttc ctttaagttt gtctggttgc
     2881 ggggcaaatt ccgatttgct ttgttttcgt aatgtgccgg gtcaagcctg tttttatatt
     2941 gcatacagct tcagggtttg ccggtggcgg ggctgacggt gaggatttcg tagccggttt
     3001 cggtcaccaa gacttcgtgt tcccattggg cggagaggga gcggtctttg gtaaccaccg
     3061 tccagccgtc gttgaggata cgcaggtggc gtttgccttg gttgatcatc ggttcgacgg
     3121 taaaaatcat acccggtttt agaacgggac cctgcccttt ttttccgtag tgcaacactt
     3181 gcggggcttc gtggaaaccg cgcccgatgc cgtgtccgca gaattcttgt acaaccgaat
     3241 aacctgcatt ttcggcaacc tgctggcagg cgtaacctac gccgcccagc gtcgcgccag
     3301 gtttgacggc ttctatgccc gccatcatag aggcgtgggt tacgtcgatc aggcgttggg
     3361 cgatggggga gactttgccg acggtaaaca tacggctgga gtcgccgtgg aaaccgtctt
     3421 ttttgatggt gaggtcgatg ttgataatgt caccttcttt gagcggcttg tcgtcgggaa
     3481 tgccgtggca gatgacgtgg ttgacggagg tgcagcagga tttggggtag ggcgggttgc
     3541 cgtagtggag gggggcggga tagccgcctt ggacgttgac gtggtagtcg taaacaagtt
     3601 tgtcgatttc gtcggtggtg atgccgggtt tgacgaattg tccgatgtag tcgagggctt
     3661 cggcgacgag tttgcccagc tcgcgcattt tttcgatttc ttcgggggtt ttgatgatga
     3721 tgccgttcat aatgcttcct tggattgcgg gtgtgcctgc cgtgccgggt caaaagaggt
     3781 tgtcgagaat ctctttatgg ggtttggccg gcgtgttttt cggggtgtcc ggtttggtat
     3841 ggttttcttt gggcgtttct ttttctttgg gtgtttcttt gggctgcgcc tgtttttcgg
     3901 gtgtttcttt tcggggttcg ggatgggggg cgtttgtggg cagtacgggg gtttctttga
     3961 gtgtgttttc agcttgtgtt ccggtgccgg ttaacgaatc ggctgcccgg gccgcatccg
     4021 aagggcgcgg tttgacccgg tttttcttgg gtgcggcagg ggacggcagt ccgctgccgc
     4081 tttcttctgt gccgtccggc tgtgcgggtt cggatgaggc ggcgtggtgg tgttcgggtt
     4141 gggcggcatc ttgtccgacg gcgccgtttg gcagccagaa ttcggtttcg cgggggctgt
     4201 cggtgtgttg cggttcggtt tggagggttt tgtcgccgtt cgggttgaat gtgctgacga
     4261 tgcctactgc caatgcgccg atggcggtga tacagatgag caatacggcg cgtatcagga
     4321 gtttgggggt cggcctgaag ggtttgttcg gtttttttgc cattttgatt gtgcttttgg
     4381 ggtgtcgggc aatgccgtct gaaggcggtt cagacggcat tgccgagtca gcgttggacg
     4441 tagttttggt agagggtaat gactttttgt acgccgacgg tggtgctgac tttttgggta
     4501 atctgcgcct gttcttcggg ggtgaggatg cccataacgt aggttacatt gccgtaggta
     4561 atgattttga cgcgcgcctg tgtagcgggg ctgatgccca gcagcgtggc gcggactttg
     4621 gacgtgttcc aagtgtcgcc ggcgatgtcg cccgcagtgc gcggcaggga ggcgacggta
     4681 atgtagttgt atacgccttc ggcggcctgt tcggaacgtg caatctgacc gacgaactgt
     4741 ttttcgcctt cggtggcgac ttgtccgagc agcagcaggt ggcggttgta gccgacgacg
     4801 gagatttggg gcgtgtagcc tttggtttgg ttgttttggc gcaggtagga acgggcggtg
     4861 gtttcgatac gcaacgccat aacgttgtca tcggtttgcg cgccggtggt tcggcggtcg
     4921 atgacggatt tcgcgccgac ggcggccccg ccgacgactg cgctgaagca gccgccgagg
     4981 gcaaggctga ggacggcggc aatcagggtg cggacggtgt gtggtttggg tttcatcggg
     5041 gacttccttt cttgggcgtt tcagacggca ttgctttgcg ccatgccgtc tgaaaagggt
     5101 tacattcctt ccagcagtac ggagtcgata cagtcgcaca tggcgtgtat cagcaggatg
     5161 tggttttcct gaatgcgggc ggtgcgcgga tgggggacgt tgagcagaac gtcggtgtct
     5221 ttgagcatgg cggcgatttt gccgccgtcg cggccggtca gggcgatgac gtgcatatcg
     5281 cgttcgtgcg cggctttgac ggcttcgatg acgttggcgg aattgccgga ggtggaaatg
     5341 ccgacgagga cgtcgcctgc acgtccgagc gcgcgcactt gtttgctgaa tacgtggtcg
     5401 aaaccgtagt cgttgccgat ggcggtcagc gcggaggtgt ctgtggtcag ggcgacggcg
     5461 gcgagttcca tgcgttcttt ctcaaaacgc ccggtcattt cggcggcgaa gtgttgcgcg
     5521 tcggcagccg aaccgccgtt gccgcaggcc aggattttgc cgtcgttcat caggcattgc
     5581 agcatcagct cggcagcctg tacggtcggc tcgaccagta ttttttcggc ttcctgtttg
     5641 gcacggatgc tttcggcaaa atgtgcggca acgcgttctt gtaatgtcgt catatcgtca
     5701 acctgtaata ttctttatcc actcgggcgg gcggtttcct tcgataagta ccgcatcgag
     5761 gcggcacggt acgtttgtca gcctgttctg ttgcagataa tactctacac ttcgttgcag
     5821 tttcaataat ttggacggag aaatgctgta tgccgcgccg ccgaaccgtt ggtttttgcg
     5881 gtattttact tcaacaaaca gaatcatgcc gccgtttttg acaatcaggt cgatttcgcc
     5941 gtaggcgcag tgccagttgc gggcaagcag cgtgcagcct tgggattgga ggaaggcaag
     6001 tgcggcatct tcccctgccg taccctgttt gtggtttaag cgcataatcg gtttaacctt
     6061 tataatgtct gtttttcaga cggcattttt tatgtttcag aaacacttgc agaaagcccc
     6121 cgacagcgtc gtcggaggga cattatacgt ggttgccacg cccatcggca atttggcaga
     6181 cattaccctg cgcgctttgg cggtattgca aaaggcggac atcatttgtg ccgaagacac
     6241 gcgcgttact gcgcagcttt tgagcgcgta cggcattcag ggcaggttgg tcagtgtgcg
     6301 cgaacacaac gagcggcaga tggcggacaa ggtaatcggt ttcctttcag acggcctggt
     6361 tgtggcgcag gtttccgatg cgggtacgcc ggccgtgtgc gacccgggcg cgaaactcgc
     6421 ccgccgcgtg cgcgaagcag ggttcaaagt cgttcccgtc gtgggcgcaa gcgcggtaat
     6481 ggcggcgttg agtgtggccg gtgtggcgga atccgatttt tatttcaacg gttttgtacc
     6541 gccgaaatcg ggcgaacgta ggaaattgtt tgccaaatgg gtgcgggcgg catttcctgt
     6601 cgtcatgttt gaaacgccgc accgaatcgg ggcaacgctt gccgatatgg cggaattgtt
     6661 ccccgaacgc cgtctgatgc tggcgcgcga aatcacgaaa acgtttgaaa cgttcttaag
     6721 cggcacggtt ggggaaattc agacggcatt ggcggcggac ggcaaccaat cgcgcggcga
     6781 gatggtgttg gtgctttatc cggcgcagga tgaaaaacac gaaggcttgt ccgagtctgc
     6841 gcaaaatgcg atgaaaatcc ttgcggccga gctgccgacc aagcaggcgg cggagcttgc
     6901 cgccaagatt acaggtgagg gcaaaaaggc tttgtacgat ttggcactgt cgtggaaaaa
     6961 caaatgatgc cggatggacc ggcaccatag tgcgaacgga atcaacggac acgccccgtc
     7021 atgcggtaca gcacgtcttt gccttgaaca cgatggacga cgaccatggc gacgtgtccg
     7081 acgacggcgg caaacagcag ccagcccaaa ttgccgtgga acgtgttgcc caagtttgcc
     7141 atccactcga ttttttccgg cgaaccctgc attacttcaa cgccgaacac tttcaacggg
     7201 ccgcgtccgc caccgtattg gcggatcatg ccgataacgg gaacggcaag catgagcaga
     7261 tacagaatgc cgtgtcctgc cgctgccgcc ttgctgtcgc tttgcggacg cttggcgcgg
     7321 ttggcaaccg cccacacgat gcgcaatgca atcaccgtca gcatgaggaa accgaaagat
     7381 ttgtgcaggc cgaacaggct gcccgcccat tccgcctctt cgtaaattgt ccacaggaca
     7441 atggtggtca gaatgccgat gaagccggca acggtcagcc agtgcagcgc gcgtgtcaac
     7501 gggtcgtaaa cggcaggttg gtttgtgttc ataaaaaaat ccttatcata tgtaacggtt
     7561 tgaacggaca ttccgtcggg tcggaatgtc aaaaaggcgc gattgtacca aagaagttgg
     7621 acatattttg tttgcaggct gaagatttgc ttaaaaattc attaagatgg gcagaacaaa
     7681 tagcttctcc gcggtatatt gaaataccgc ttatacctta taatgtcggc agaaaatctg
     7741 ttcagacggc atcaaaacat atggcaatcc gcctgaaacc ctatttaaaa ccaacaaaca
     7801 agagcattta atgaaattca tcgacgaagc aaaaatcgaa gtcgccgcag gcaaaggcgg
     7861 taatggcgca accagtttcc gccgcgaaaa attcgtaccc cgcggcggcc cggacggtgg
     7921 cgacggcggc aaaggcggca gcgtctgggc agaagccgac gaaaacacca acaccctcgt
     7981 cgaataccgc ttcgtcaaac gctaccaagc caaaaacggc gaaaaaggcc acggttccga
     8041 ccgctacggc gcaggtgcgg acgacattgt cctcaaaatg cccgtcggca cccttatccg
     8101 cgacctcgac accgacgaaa tcgttgccga cctcacctac cacggacagc gcgtctgcct
     8161 cgccaaaggc ggcaaaggcg gcttgggcaa catccacttc aaatcctccg tcaaccgcgc
     8221 cccaaaacaa tccacgcccg gcgaagaagg cgaaacccgt tccctgcaac tcgaactcaa
     8281 agtcctcgcc gatgtcggct tgttaggcat gcccaacgcc ggcaaatcca ccctgattac
     8341 cgccgtctcc gccgcacgcc ccaaaatcgc caactacccc ttcaccaccc tgcatccgaa
     8401 cttgggcgtg gtacgcatcg acgaaaacca cagcttcgtg atggccgaca tccccggcct
     8461 gattgaaggc gcggcagaag gcgcaggcct cggccaccgc ttcctcaaac acctatcacg
     8521 caccggtctg ctgttacacg ttgtcgattt ggcacccttc gacgaaaccg tcaatcccgc
     8581 cgaagaagca ctcgccatca tcaacgaatt acgcaaatac gacgaagaac tttacggcaa
     8641 accgcgctgg ctggtgctga acaaactcga tatgctcgac gaagaagaag cccgggcgcg
     8701 aacagccgcc ttcctcgaag ccgtcggctg ggactacccc gaacctgatg accgtttcca
     8761 attcgacatg gaaaccccgc gcctcttcca aatcagcgcg ctgacacatc aaggcacgca
     8821 ggagttggta caccaaatca accaatacct ggccgagaaa aaacgtatcg aggctgaaaa
     8881 agccgaagcg gagaaagcag cggcaaatgt tgaaattatc gaacagcagc ctaaaacgga
     8941 tactggcgtg tttaagccgg agtaaaagga caaggccgtc tgaaagtttt caggcggctc
     9001 attctgcaga gatgtgctat gtacaaaaca atagatttat tttcaggcat tggcggaata
     9061 cgcctaggct ttgaaaaata cgggtgcacc aatgtattct catcggaatg ggacaaatat
     9121 gcccgacaag tgtatgaagc aaactttgga gaaaaacctt ttggcgacat taacggcatc
     9181 gatccttcag atattcccga tcatgatatt ctgcttgcgg gatttccgtg ccaacctttc
     9241 agtatcgcag gtaagggatt gggatttgaa gatacgcgcg gaacattgtt tttcaatatt
     9301 gccgaaatcc tgaaaaccaa acagccgaag gcttttcttc tggaaaacgt taagcggctg
     9361 actacgcacg attcgggcag gactttccgt attgttttag aaacccttaa acagcttggt
     9421 tatacggttt attttaaggt tttaaatact ttggatttcg gattgccgca aaaacgcgag
     9481 agaatttata ttgtgggttt ttcagacaac atcccttttt actttcccga acccataaac
     9541 caatatcggc cattgggcga actgctggaa aacgatcgag atgtcgaacc aagctatttt
     9601 ttatcagata ccttgaaaca aaaacgtttg gcagccttaa aaaaagctcc gcccacccca
     9661 tcaatttggc atgaaaatat tggcgggaat gtttccgcac ttccatattc ctgtgcctta
     9721 agggccgggg gaagttacaa ttatttggtg gtaaacggag taagaaggct aacaggaagg
     9781 gaaatgttaa gattacaagg ttttcccgat gattttgaaa tcaatatccc ctactcgcaa
     9841 gtcaggaaag tcgccggaaa ttctgtatcc gtccctgtaa tagaagccat cgcggaaaat
     9901 atgctcgctt ctctttccgg caaggtcgaa caaaaagggc aattggattt attggaggca
     9961 ggatgacgct tgaagaacaa caagccaaag aagcattgga cggcattatc aaaaaatccc
    10021 gtgtccacct ttataaaccc attcaaattg cagaaatttt atatcatgac cgttgtataa
    10081 aacagttgga ttttttaaat ttagatactt accgcaacca atctaaacgc tggagggatg
    10141 aaatctgccg tcggttttta gggcggattt ctacttcatc tgcaaaattc caagataatc
    10201 tgtttgaaaa aaatgcaata ccgcctgaaa aactagcggt attgggaaca ttaaacagac
    10261 agtcggacgg cggggtagaa tcgtacattt ataaacagtt tttcaatcgt ttttcccaaa
    10321 tgagcgaagc attggcttat gtcggcaata cagacagata ctccttccaa ctgtctgaat
    10381 ttctaaattt attttggctc gagccgggat tgaaaagaag tatagacaag atatatgaaa
    10441 tcgttgttta tgcgttgttt gatgcattgg tttcagaatt aggcataacg gtttcaattg
    10501 attttcctaa ggaaaacctg tttttatggg aggaatatca agattttgcc gaaaaaatca
    10561 tcactatgcc gaaaaatgag catttaaaac ttcctgcaaa aatccatcgt gtaggcgtaa
    10621 ccaatgctgc cgatagaggg ttggatatgt ggtctaactt cggattggcc atacaggtca
    10681 aacatctctc tttagacgaa gagttggcag aagatattgt atcctccatt agcgcagata
    10741 gaattgtcat tgtctgcaaa aaggcagaac aatcggtgat tgtttcatta ctgacgcaaa
    10801 taggctggaa aagccgaatt caaaacatcg taactgagga tgatttgata agttggtacg
    10861 aaaaagcatt gagaggccaa tatccgattg cagaagcgtt gttggaaaat attaaaactg
    10921 aaattatgcg tgaatttcct gccgttaatg aagccaatga atttttagat ttcgcccaaa
    10981 atcgcggata tgacattact gttactcatt tctaaactta cttgatgaat atgaccgcca
    11041 tctacaacac ccttacccgt caaaaagaac ccttcgcccc catcgaccct gaaaacgtgc
    11101 gtatgtacgt ctgcggcatg accgtttacg actattgcca cttaggccat gcccgtgtga
    11161 tggtggtgtt cgacatgatt gcccgctggt tgcgcgagtg cggttatccg ctcacttatg
    11221 tgcgcaacat caccgacatc gacgacaaaa tcatcgcccg tgcggctgaa aacggcgaaa
    11281 ccatcggcga actgaacgcg cgtttcattc aggcgatgca cgaagatgcc gatgctttgg
    11341 gcgtgttgcg tcccgacatc gagccgaagg cgacggaaaa catcccgcaa atgattgcca
    11401 tgattgagac cctgattcaa aacggcaagg catatcctgc cgcaaacggc gacgtttact
    11461 acgccgtgcg cgagtttgcc gcttacggac aattatcggg caaatcgttg gacgacctgc
    11521 gtgcgggcga acgcgtggaa gtggacggtt tcaaacgcga tccgcttgat tttgtattgt
    11581 ggaaagcggc gaaagcaggc gaaccggcgt gggaaagccc gtggggcaac ggccgtccgg
    11641 gttggcacat cgaatgttcc gcgatgagtg aaaacctgtt cggcgatacg ttcgacattc
    11701 acggcggcgg cgcggatttg cagttcccgc accatgaaaa tgaaatcgcc caaagcgtcg
    11761 gtgccagcgg tcacacctgc ggtcatgacc acgcgcaaac ccaccacggt caaagtattg
    11821 ccagccacgt caaatactgg ctgcacaacg gcttcatccg tgtggacggc gaaaaaatgt
    11881 ccaaatcgct gggcaacttc tttactatcc gcgaagtatt gaaacaatac gaccccgaag
    11941 tcgtgcgttt cttcatcctg cgcgcccact accgcagccc gttgaactac tccgacgcgc
    12001 atttggacga cgcgaaaggc gcgttgaccc gtctgtacac caccttgaaa aacacaccgg
    12061 cggctgagtt tgatttgtct gaaaacgcca acggctacac ccgccgtttc tacgccgcca
    12121 tgaacgacga tttcggtacg gtcgaagcgg ttgccgtgtt gtttgagctg gcaggcgaag
    12181 tgaacaaaac caatgacgca cacctcgccg gctgcctgaa agctttgggc ggcatcatcg
    12241 gcctgctgca acgcaacccg attgagttcc tccaaggcgg cgcagtttca gacggcctct
    12301 ccaacgaaga aatcgaagac ttgattgccc gacgcaaaca ggctcgcgcg gataaaaact
    12361 gggcggagtc cgaccgcatc cgcgaccttc tgaacgaaca caaaatcatt ttggaagaca
    12421 gcgccggcgg cacgacttgg cggcgcggtt gaactgtatc ggaaagggca aaagtaccgc
    12481 cttttgccct tttttcgttt tcagacggca tttcccgctt gaagccccgc cgttttcagc
    12541 aggcaggacc gaatgggaaa taccgggcgg cgcaccgggt gccgctttgt gtatgggaac
    12601 attgtgagaa cttattttta ttatgcttaa aatcatttcc gccaacgtca acggcatccg
    12661 ctccgcctac aaaaaaggtt tttacgaata catcgccgca tcgggcgcag acattgtctg
    12721 cgtgcaggaa ctcaaagcgc aggaggctga tttgtctgcc gatatgaaaa atccgcacgg
    12781 gatgcacggc tattggcatt gcgccgagaa gcgcggttac agcggtgtgg cggtgtacag
    12841 caaacgcaaa cccgacaatg tgcaaatcgg catgggcatt gaggaattcg accgcgaagg
    12901 gcggtttgtg cgttgcgatt tcggcaggtt gggcgtcatt tcgctttatt tgcccagcgg
    12961 cagcagcgcg gaagaacgcc agcaggtgaa ataccgtttc ctcgatgcgt tttaccctat
    13021 gctcgaagcg atgaaaaacg aagggcgcga catcgtcgtc tgcggcgact ggaacatcgc
    13081 ccaccaaaac atcgacctga aaaattggaa aggcaatcag aaaaattcgg gcttcctgcc
    13141 cgaagagcgc gagtggatag gcaaagtcat gcatacgctg ggctggacgg acatgtggcg
    13201 cacgctctat cccgatgtgc cgggttatac atggtggagc aaccgcgggc aggcgtatgc
    13261 gaaagatgtc gggtggcgca tcgattatca gatggttacg cccgaacttg ccgccaaagc
    13321 cgtgtccgca cacgtttata aagatgaaaa attctcggat cacgcccctt tagttgtgga
    13381 gtatgactat gctgccgaat aaggttttgg gtaaatatga ttggaatgtg gacggaaaaa
    13441 caggaatagg cgcggcatgg gttgtcgctg cgtttatcct gcccatattg gtgtgggcgg
    13501 tgtttatgtt gtcgcggatg caaggctggc ttgcgccgac caaggcaaac ccgatatggg
    13561 cgttggtgtg gctgctgatt tgtctgccct gcctgctgat tgcggccaaa tgtttgggtt
    13621 ggaaaggctg gcggcgggtg gtgaatattt ttgtctgcct gaccgtctgc gccatattga
    13681 gcgtacccgc ttcgctgctc atcgccttta ccctccggga cctgctcaaa tagtcgcgga
    13741 caatggaaag tttcgagctt gaggatttga cgctgtggct gatacgcgat gcggatgaag
    13801 cggaaatgtg gatagaccgt tgggcggtca gttatcccgt tgtgcagatg tccgaagcgt
    13861 ccgcaaacgg cagcacggaa gcgtggcaaa gcgcgcttca gacggcattc gaacggatac
    13921 agggcaggca tatcgccgtt gtcgcacacg gcgcgggcgc ggccgcattc ttggcgtggc
    13981 tgtaccgcgc cgacatcctg acgcagaaga aactcgccgg tatcattctc gtatctccgc
    14041 gtcccgatat ttttcccgac gatgcggaac acactttcca acgcgtccgc tgtccttgcc
    14101 gtgccgcatt ggtggtgtcc gaacacggcg gcgtgccgca cggttgggcg caaaaacagg
    14161 cggatttgtg gaacgcccgc ctgttggttt ccccgcattc gggcagtttg aacggtatgc
    14221 tcggcggctg gcagtggggt atgaagctga tgcaggaaat gttgctggcg tgaacggtcg
    14281 ctttatcgga atttcggtta accggggttg caaggaaaat accgtctgaa acaggcttca
    14341 gacggcattt ccattcaaac agggcttaca ggctgcccaa cacgatacgc aacacgcggc
    14401 gcatcggttc ggctgcaccc cacaaaagtt ggtcgccgac ggtgaacgcg ctgatgtatt
    14461 cgccgcccat gcccagtttg cggatgcgtc cgacagggac ggacagcgtg ccggtaactt
    14521 tggcgggcgt cagctcgcgg atgctggctt ctttttcgtt ggggacgact ttcacccagt
    14581 cgttcgcgcc tgccaaaatc gcttcgattt cggaaacagg caggtctttt ttcaacttca
    14641 gagtgatggc ttggctgtgg cagcgcatcg cgccgatgcg gacgcacagg ccgtcgatga
    14701 cggtcggatt gccgctgcgt ccgaggattt tgttggtttc cacgccgccc ttccattctt
    14761 ctttggactg gccgttgccc aaatccacgt caatccacgg aatcaggctg ccggcgagcg
    14821 gtacgccgaa gttggctttc ggatagtctt cgctgcggag gaaatcggac actttgcggt
    14881 cgatatcgag aatcgcgctg gaaggatcgg caagctcgtc cgccacttgg gcgtgaatcg
    14941 cgcccatgcc gctgatgagt tcgcgcatat ttttcgcgcc cgcgccggaa gcggcttggt
    15001 aggtcatgct ggtcgcccat tcgaccaaat cgttttggaa caggccgccc aaagccatca
    15061 gcatcaggga gacggtacag ttgccgccga tgtagttttt cacgccgttt ttgagaccgt
    15121 tgtcgatgac gttgcggttg acggggtcga gggcgataat cgcgtcgtct ttcatgcgca
    15181 gtgaggacgc cgcgtcaatc cagtagccgt tccagccgct gtcgcgcagg gcttggaaga
    15241 cggatttggt gtaatcgccg ccttggcagg tgacgataat gtccattttc gccagctcgg
    15301 caacgtcgtt cgcgtccaat aatgttttag ccgcctgacc gaaatcaggt gccgcgccgc
    15361 cgacgttgga agaggtaaag aaaaacgctt cgggaatgtg ggcgaagtcg ttttcttctt
    15421 tcatacgctg catcaaaacc gaaccgacca taccgcgcca gccgacgaaa cctactttca
    15481 tgtattgctc cttgaggaaa atcgttaata aaattatgta tggcgtaaag aaagcgtttt
    15541 aacgccgtat tgggaaaatg taaagttctt tatcgaattt tctttttggg attggcgttt
    15601 gcagcagtag ggcggtagaa ttccatctgt ttaagaacaa ttatcaggaa cggatatgga
    15661 agaaaaaaac gactacaccg acgcggtatc cgacaaccgc aacgggcagg aaattgaagt
    15721 cggaattgcc ggcgcaggcg accggatact tgccgcgctg ctgaaccaat tgtttacctt
    15781 tttgattttg ttggtgccgt ttgtcggact gattgccttc gccgtcaaaa atgaaggcag
    15841 gattggcggt cgtgaggaaa tattcgggct gcttttgggc atgacttcct tttgggtcgg
    15901 actggcgggc attttggcgt acaccgtcat ccaaatctac tatatgagca gggacgggca
    15961 gtcattgggt aagaaaatca tgagaatccg cgtgttgaaa accgacggcc gcaatccggg
    16021 ttttgtcggc acggttttgg tacgcgaaat cgcgtggtcg gttttggttg ccattattgc
    16081 cgccgttatc ggtcttgcag taggtgaaaa cggggaaaac gccatcaacc tgctggcatt
    16141 ccttgccaac tttgtcctgc tctttatggt caaacgcgac cgccgcacgc tttacgacat
    16201 attggcggat acggttgtcg tcaagctgcc caaataaacg gacgcggcaa aatgccgtct
    16261 gaaaggcttt cagacggcat ttttatcttc agaaccgctc aagcatcgtg cagcgaagct
    16321 gcgtgcaaag tgttttccat taatgtggca atcgtcatcg gacccacgcc gccgggaacg
    16381 ggcgtaatca tcgccgcccg ttcttttgcc gtttcaaatt ccacgtcgcc gcacaggctg
    16441 ccgtcgtcca aacggttgat gcccacatcg ataacgaccg cgccgggttt gatccacccg
    16501 cctttgacaa agttcggaat gcccacgccg accaccaaaa tatcggcggc ggcaacttca
    16561 tcggcaagat tttcggtcgc actgtggcag accgttaccg ttgcgcgcgc cagcagcaat
    16621 tccaacgcct gcgggcggcc gacaatattc gacgcgccga ccacgaccgc ttttttcccc
    16681 ttcggatcaa tgccgtaagc ctccaaaagc gtcatcacgc ccttgggcgt acacgggcgc
    16741 atcagcggca ttttgaccgc cagcctgccg acattgtaag gatggaagcc gtccacgtcc
    16801 ttatccggcg aaatacgttc caagatcgct tggctgtcga ggtgcttcgg cagtggaagc
    16861 tgaaccagaa taccgtccac ttcggaatcg gcattcagac ggtcgaccaa tgccagcagt
    16921 tcttcctgcg atgttgattc gggcagttcg tacgacagcg acttgatgcc gcatttttgg
    16981 caggcggttt tcttgttgcg gacataaact gcgccggcag ggtcgcctcc gaccaaaacc
    17041 acggccaggc aaggcgtgtg cagattgtcc tgttggcgtt gcgccaccgc ttcggcaatc
    17101 gcctgcaggt gtttttgcga aacttcttta ccgttgatca gttgtgccga catatccgtc
    17161 cttttgttaa acaggtagaa aatgctgaaa attatcgcat tttccgtttg tttttacagc
    17221 agaatgccga aaataaattt gccccgaaat aaaatacggc tgtttggacg gattaaatat
    17281 tttttcaaat caaaaaacta tatttttcat cttggtgttt tgaaaataaa tgttgacgca
    17341 tctttccctg aactgtatat tccgcttctt caagtcgggg cgtagcgcag cccggttagc
    17401 gcatctgctt tgggagcaga gggtcgtgag ttcgaatccc accgccccga ccaatcaatc
    17461 agcacgaatc ggcatgagcg cccgtagctc aaccggatag agcaccgacc ttctaagtcg
    17521 ggggttacag gttcgattcc tgtcgggcgt gccaagaatt tgattgaaaa ccgtttttat
    17581 ggtggctgta gctcagttgg tagagccccg gattgtgatt ccggttgtcg tgggttcgag
    17641 ccccatcagc caccccagat aaaggcccgt acttttcggt acgggtcttt tcctgtttgc
    17701 gcgtactgtc cgcgccgtcg taccggtgcg gcggtatgcc gtctgaaagc cgggccggtc
    17761 atacggaaac cgcttcatac ggcatttgcg ccgccggctt tgcctttcgc atatgcttct
    17821 gtatcatgtg ggtttttctg tcttttatgg tttttgtcgg aggctttgat ggttgacgct
    17881 tggtctgtcc ctgattttga atcgaaaatc tgtccgcccg aggcgttggc ggcgcgtttg
    17941 gcgttgttgc cgcgcccgct ggtgtttacc aacggctgtt tcgacatcct ccacaggggg
    18001 cacgttactt atctggcgca ggcgcgttcg gcaggggcgg cgttggtgct ggcgttgaat
    18061 accgatgctt cggtgcggcg tttgggcaag ggcggcgacc gcccggttaa ttctttggag
    18121 aaccgtgccg ccgttgccgc cgcgttggaa agtgtggatt tggttacttg gtttgacgag
    18181 gatacgccgg cggcgttgat tgaggcggtc aagccggagg tgttggtcaa gggcggcgat
    18241 tgggtcgtgg ataagattgt cggtgcggcg gaaacgctgg cacgcggcgg tcaggtgttt
    18301 tcaattccgt ttctgcatca gacttcgaca acgaagactt tggcaaaaat ccgtgcggca
    18361 gagggcggaa aatgacggtt ttgaagcctt cgcactggcg ggtgttggcg gagcttgccg
    18421 acggtttgcc gcaacacgta tcgcaattgg cgcgtgaggc ggacatgaag ccgcagcagc
    18481 tcaacggttt ttggcagcag atgccggcgc atatacgcgg gctgttgcgc caacacgacg
    18541 gctattggcg gctggtgcgc cccttggcgg ttttcgatgc cgaaggtttg cgcgatctgg
    18601 gggaaaggtc gggttttcag acggcattga agcacgagtg cgcgtccagc aacgacgaga
    18661 tactggaatt ggcgcggatt gcgccggaca aggcgcacaa aaccatatgc gtgacccacc
    18721 tgcaaagtaa gggcaggggg cggcaggggc ggaagtggtc gcaccgtttg ggcgagtgcc
    18781 tgatgttcag tttcggctgg gcgtttgacc ggccgcagta tgagttgggt tcgctgtcgc
    18841 ctgttgcggc acttgcgtgc cggcgcgctt tggggtgttt gggtttggaa acgcaaatca
    18901 agtggccaaa cgatttggtc gtcggacgcg acaaattggg cggcattctg attgaaacag
    18961 tcagggcggg cggtaaaacg gttgccgtgg tcggtatcgg catcaatttc gtgctgccca
    19021 aggaagtgga aaacgccgct tccgtgcagt cgctgtttca gacggcatcg cggcggggca
    19081 atgccgatgc cgccgtattg ctggaaacat tgcttgcgga actgggcgcg gtgttggaac
    19141 aatatgcgga agaagggttc gcgccatttt taaatgagta tgaaacggcc aaccgcgacc
    19201 acggcaaggc ggtattgctg ttgcgcgacg gcgaaaccgt gtgcgaaggc acggttaaag
    19261 gcgtggacgg acgaggcgtt ctgcacttgg aaacggcaga gggcgaacag acggtcgtca
    19321 gcggcgaaat cagcctgcgg cccgacgaca gaccggtttc cgtgccgaag cggcgggatt
    19381 cggaacgttt tttgctgttg gacggcggga acagccggct caagtgggcg tgggtggaaa
    19441 acggcacgtt cgcaaccgtc ggcagcgcgc cgtaccgcga tttgtcgcct ttgggcgcgg
    19501 agtgggcgga aaaggcggat ggaaatgtcc gcatcgtcgg ttgcgccgtg tgcggagaat
    19561 ccaaaaaggc acaagtgaag gaacagctcg cccgaaaaat cgagtggctg ccgtcttccg
    19621 cacaggcttt gggcatacgc aaccactacc gccaccccga agaacacggt tccgaccgtt
    19681 ggttcaacgc cttgggcagc cgccgcttca gccgcaacgc ctgcgtcgtc gtcagttgcg
    19741 gcacggcggt aacggttgac gcgctcaccg atgacggaca ttatctcggc ggaaccatca
    19801 tgcccggctt ccacctgatg aaagaatcgc tcgccgtccg aaccgccaac ctcaaccgcc
    19861 ccgccggcaa acgttaccct ttcccgacca caacgggcaa cgccgtcgca agcggcatga
    19921 tggacgcggt ttgcggctcg ataatgatga tgcacggccg tttgaaagaa aaaaacggcg
    19981 cgggcaagcc tgtcgatgtc atcattaccg gcggcggcgc ggcgaaagtc gccgaagccc
    20041 tgccgcctgc atttttggcg gaaaataccg tgcgcgtggc ggacaacctc gtcatccacg
    20101 ggctgctgaa cctgattgcc gccgaaggcg gggaatcgga acacgcttaa aaaatatgcc
    20161 ttttttcaac acggggcggc atccgtccga atctgtccgg attctgccgc ccgacagact
    20221 gccgcccggg cgggcggggt ttcaaacatt gaggaaattc taatgaaatg gctcttcacc
    20281 cttttggcgg tactcaatat tgccgtattc ggcggcacgg tgggctataa attggccgtt
    20341 aaagcggcgg gcggcgtgcc ggaaaaccgg gccgtcgaaa acacgccccc cgcaacgccc
    20401 gccgccggca atgcggcggc atccgtggag gatacggcgg cactgctcaa accgggcgac
    20461 atcctgagcg aagaacaggc ggagcagttg cgcttgaaaa aagaagcgga acagaaaaaa
    20521 ctgagggaga aaaaacagcg tgaagaaaaa gcccgccgcg aaaaactcgc cgccgaaaag
    20581 gcgcaggcgg aacgtgaaaa cggcgcggcg gatgccttat gcgccgcgca ggcaagcctc
    20641 acgatggacg aagacgacta ccaccgcatc aaagggcttt tgggcaaatg gtcgcacgtt
    20701 gccagcagga gcgtcgaaaa acgcaccgcc aaagccaaac ctgccgacaa aacctaccgc
    20761 gtcgtcctgc ccgtttccgc cgatgccgaa aatcaggcgg cggagctgtc tgccaaaggt
    20821 ttcaacccca taccgtttga cggcgcattg agtttgggtg tcggcaacag ccgggaaaac
    20881 gcccaagccc tgcaaaaccg acttgccgat gccggattcg gcggggcgca tattgtcgaa
    20941 cactttgccg aagccgacag gcaggacgat tctttgagcg tgtcgcgtat gacggttttg
    21001 tttacgggcg tgaatgccgc cgatgcggac gaaatccgta aaatcacgtc cctatacggc
    21061 aaactgaacc tcaagtcttg caaataagtg cagaccaaaa acgccaaacc caccgctagg
    21121 gtgggtttgg cgtttcggtt ttgagtatct gattaaaaag ggaatgtatg tctcgtatat
    21181 gccgactcca agtgtgaaag tgaattgtga gggaatacag tgtttaatgg ttcggaacgg
    21241 acggcttccg agtggttttg attatacgct aataataata attatcaata aaaatttaaa
    21301 aataaatgcg gattttttgt aatgcgatga aaaataaaca aattaacctg tccgccgatg
    21361 tttgtgattt gggtcggcaa actttcattg cgccggacaa atcggggtcg gcaggcatgg
    21421 acgggggctt cgtgcctgtc ggaaagaagc ctgccgtgtt tgaacaaccg tccgatttgg
    21481 tgcggacagg gttgccattc gggggtgttt gagcgcggat tcggggatgc aaaagcggtg
    21541 aaaccgttgc ggatgaaggc ttacggatgc cccgaggtat ttttaaatgt attcaaacga
    21601 caaaacaccc ttctattgcc gtacttaggg cttgcgcagc agcctgcgcg ccggggagtc
    21661 cgggtgtttc gttggttgcg gtgtcggggc gggcggcaag gttttccggc cggtttccct
    21721 gttcctcacg acaaagccgt gatgccgggg aatggcggtt tgcccgtttg gggccgcgat
    21781 gccgtctgaa gccttcagac ggcatttttg ccgccttttt caatattccg ccgaatgcca
    21841 aaacggttgc ccgactgtcg gcgtgctggc ttgggctttg gttcgcgctt cgaccggccc
    21901 ggcttcaaat gccagccgtc cggattcgac ggcgagtgcg aaggcgcgcg ccatgttgac
    21961 ggggtcgccg ctgcgggaaa cggcggtgtt taacaatacg ccgtcaaaac cccattccat
    22021 cacttgtgcc gcttgggaag gcaaacccaa gcccgcgtcg ataatcagcg gcgtgtcggg
    22081 caggcgttcg cgcaggattt tgagcgcata ggcgtgaacc gcccccaaac ccgtgccgat
    22141 gggagccgcc cacggcatca acgcctgaca gcccgcatcg agcaggcggc ggcaggcaat
    22201 caggtcttcg gtgcaataag gcaacacttt gaagccgtct ttaatcagga tttccgccgc
    22261 ttcgacgagt tggaacacgt ccggctgcaa ggtgtcgtcg tcgccgatga gttccaattt
    22321 tatccaatcg gtttcaaaca cttcgcgcgc catttgcgcc gtcgttaccg cttcctgcac
    22381 gctttggcag cctgccgtgt tcggcaggac gggaacgccg gtttcttgaa gcagcgacca
    22441 aaacccctga ccgtgcgcct cgccgccgca tcccgtgcgg cgcagcgaga cggtaatcat
    22501 cgcgggccgg gcggttcgga cggattgttt gaggatttca ggggtcgggt aggcggctgt
    22561 gccgagcagc agccgcgaag ggaaagtttc gccgtacagg gtgagcatga tggattcttt
    22621 ggtatggttg tcggacgcga tgccgtctga aagccggcgg cgtgcctttg gggtttcaga
    22681 cggcattcag ccgccgacga cggggcggac gatgtcgatt ttgtcgtttt cgtgtaaaac
    22741 ggtttccgta tacgcgcctt tggggatgaa aacggtgttg accgccacgg caaagggttt
    22801 ttgcggcgcg gtttgggcga tgaggtcggc aacgcttgtg ccgtgaagtt cggcgggtcc
    22861 gccgtttaag atgatgttca tggtttctcg atgtctctgt atttatagcg gattaacaaa
    22921 aaccggtacg gcgttgcccc gccccggctc aaagggaacg attccctaag gtgcccaagc
    22981 accgggcgaa ccggttccgt actatttgta ctgtctgcgg cttcgccgcc ttgtcctgat
    23041 ttttgttaat ccactatatc tagccgaatt actttatttt ttgatacgca accggccggt
    23101 tgccgtcatt cccgcgcagg cgggaatcca tttttgaaat tcggaaactg tttttcaaat
    23161 gatggtttct caagttttac ggcggattcc cgcctgagcg ggaatgacgg aatttgatga
    23221 tatgccacat ttaaagttaa gcatatttgc gataacgggc taagttttgc ctacacggca
    23281 attgaatttt cttctttcgg ttttatccat cccacaaagc ctgaaacgct ttaaccaccg
    23341 cttcgggatt tgccgcttcg gttacggcgc ggacggcggc gagtgaggaa actccggtgg
    23401 ccagcacggc tcgggcgttg ttcaaatcga tgccgccgat ggcgacgacg ggcgtgccgc
    23461 gagcctgttc cacatattcg cgcaatttgt ccaagccttg cggggcggtg ggcatttgtt
    23521 tggtcgtggt ctggaaaatc gcgccgctgg cgatgtagcc ggggtgtacg gacagggcgc
    23581 ggtcgagttc ggcgacggag tgcgtactca aacccaaacg caaaccggcg gcggcgatgg
    23641 cggcaaggtc ggcggtgtcc atgtcttcct gcccgagatg tacgccgtat gcgcccgctt
    23701 cgattgcctc gcgccagtgg tcgttgatga aaagctgtgt gcggctgcct tgacaggcgg
    23761 cgacgcagcg ggcgatttcg cgtttcaatt cgttgccgtg caaggtttta cagcgcagtt
    23821 gcaccgtgtc ggcacctgct ttgaccatgc gccccaccca atcggcggtg ggaacgacgg
    23881 cgtagaattt aagtagggat tttaggggcg ggaaggtcat agggtgtcct ttcgggcggc
    23941 tttaatcttg tcttccgata tacgccaaac cgctttcttc atcacgttcg ggcgcgtctt
    24001 ttccgtcaaa cagtgccact gccaatctga cggcggcggc ggttacggcg ggggaaatca
    24061 taaagccgtg ccggaaaagg ccgttgattt cgatgaggcg gcgttcgcgg ctgtagcgga
    24121 tttcggggtt gtggtggttg agcgtggggc gcaggccggc ggcgatttcg aggatgtcgg
    24181 cttcgccgaa ggcggggtgg acggcataga gcgcggataa gagttccagc ccggaacgta
    24241 cgctggcggg ggcttggctt tcgctttcga tttgggtcgc gccgatgacg aagacgtggt
    24301 tttctttcgg ggcgatgtag agcggatagc gcgggtgcag caggcgcacg gggcggttga
    24361 gcgtgatttc gggcgtgtaa acccgcgcca cttcgccgcg tatgccgcgc aaggtgctgg
    24421 tgtgctcggg ggattggttc cacgcggttt tcgcgccgta gccccggcag tcgattaccc
    24481 agtcgtattg ggcttgcagg tcttgggggg cgcattcgtg ttcccaatgg caagggacgt
    24541 tcagttcgtc caaagcgtcg gcaagtgcag acaatatttg ccgcccgtcg agctggcctt
    24601 cggtcggcag gtagatgccg tctgaaaaac gtccgccgag ttgcggttcg cgttcggcga
    24661 tttcatcggc gcgccaacgg acgatttcgt catccgctac gccgccgcgt ttgagatggc
    24721 ggacgaactc gctggataat ggcttgtcct gcccgtgcca cacaatcagg ctgccgtttt
    24781 cctgcatcat cgtgtgcgtg ttcagacggc atcggatgcc gcgccaaagc ggaatgctct
    24841 gcctgcccag cctgatgact tcgggcgttg cctcgaccgc ttccgccgca ggcgcgagca
    24901 tcgcggcggc aacataggcg gcggcgtgtt cgccttggcg ggtgcccttg tcgaaaagtt
    24961 caatctgata accttgttct gcaagctgca atgcggtcag ccttccggaa aggccgcctc
    25021 cgaggacggc gatacgggtc atgacgggtt cctttgtaaa gattgggttt ttttaaagaa
    25081 aaggcgtacc gatacggtgg caatggcaac ggcagacatt acgggggcgg tcagaccgat
    25141 gctgctttcc caaccggacg agagcaggaa gcggtagagg atgaagcctg ccagccacag
    25201 aaccagtccg gcaaagtcaa agccttcaat ctcctcacgc cgttttaaga cgaaaaagtc
    25261 ggcaatcaaa accgccgcca tcggcgcaaa taccgagccg ataagcagca ggaagttttc
    25321 atattcggta acgggcagca tgacggcaag caccgtgccg atcagggtaa cgccgacagc
    25381 gacgggtgtt tccgcaaaac gcgcggaaat gttgttcgca ctcgcgccgg cggaataggt
    25441 atcgagaaac gttgtggtaa cggtggagag gacgactgcc agaatgcccg ttatgcccaa
    25501 gcccgcgccc aacaggattt tcgccacgtc ggtttctccg gtaaacagag ccgccgccaa
    25561 acccaaggca tacatccagc agcccgtcag cgtataggcg agcgttgccg tcagggttgc
    25621 cgcaaacggg cggcgtgctt ggcgcgtgta gtcggcggcc agcggcagcc aggaaagcgg
    25681 catgacggcg gacagttcga ctgccgttcc gaaggtcatg ccgtctgaaa cggcgggcgc
    25741 ggcgtttgtg ccggacgaag cgaacacttc gacgctcaac cacaacacgg caagcagcat
    25801 cagcagcatc gaaacggttt tcagcccgcc cgttctgcgt gcgccgaaaa ccagccacag
    25861 cacgatcagt gcgccgtttg ccaatgccca ccagacaaag gattcgccgt cccacaacac
    25921 tttgcccaaa gcggagctga ccgttgcgcc gacgtaaatc atcaccgccg tccagccggc
    25981 cagttgcagc atattcgcca cggaaaacag cactgaaccg catttgccga acgacaggcg
    26041 cacactttcc atcgagctgc gtccggtcag tgcgccgata tacgctgccg caaaaaacag
    26101 cgcgccgccg acggcatgac ccaaaagcag ggccgccaga ccgcgctgcc agcccaaggg
    26161 ggcgagcagc gtacccgtgc tgatttcggc aatcgatacc gccgcgccga accaaatcag
    26221 cccgatggcg gcggaagatg aaggagagga ggcattgccc gacatatgac gtcctttaaa
    26281 aagaaggaag gtgggcgggg aagtgcggaa aagggaacgg aagcccgaaa gggcggggag
    26341 gttttgtttc ctacgcaggc attaccccga caggttctac ggattttgct ttcgcaatct
    26401 cagccgcccg gcgggcggca ctccgacaag aaggctttaa gtttacacaa agcaacgtgc
    26461 gttggcaaaa ttcttacatt ttcgacggca cggcgcattt ccataacaaa tgccgtataa
    26521 tcaagcccct gattttttat gcgccgacac tatgcatccg acctattccg ccgtacaggc
    26581 gcggctgctc gaagccaacc gcctttgccc cgaactgctc gccaaaagcc tgtgcatcat
    26641 cggcgcgcac cacgtcgatt atgccgatat ctactgccag cgcaccgctt atgaaagctg
    26701 gcatttggaa gagggcatcg tcaaatcggg cagcttccaa atcgatcagg gtgtgggcgt
    26761 gcgcgccgtt tcgggcgaca aaaccgcctt tgcctacgcc gacagcctgt gcatcgattc
    26821 gataaaccgt tccgcccgcg ccgtccgcgc gattggggcg gcaggcggca aggtgtccgc
    26881 caaaatgccg tctgaaacgc gcggcaagcc ggtttgttcc gcatccgacc ccattgccgg
    26941 cctcgattcc gccgccaaag tcgcgctgtt gaacaaagtg gaagcaatcg ccaaagccgc
    27001 cgatccgcgc atcgtgcaag tgatggccgg tttgacctgc gaatacgata tggtttacct
    27061 cgcccgtctg gacggcaaac acgccgccga catccgcccg atggtgcgcc tgaacgttac
    27121 cgtcatcgcc aaacagggcg aacgccgcga acagggcggc gcgggcggcg gaggacgcta
    27181 cgacttggct tatttcgatg aaaccttggt gcggcagttt gtcgatgccg ccgtcaaaca
    27241 agccctgacc aatctcgaat cccgtcctgc gcctgccggc gagatgaccg tcgttttggg
    27301 caacggttgg ccgggcgtgt tgctgcacga agcggtcgga cacggtttgg aaggcgattt
    27361 caaccgcaag gggaccagcg tcttttccgg ccggatcggc gaacgcgtcg ccgccaaagg
    27421 cgttaccgtc gtcgatcagg gcgatattgc cggccggcgc ggttcgctca acatcgacga
    27481 cgaaggcaac gaaacacgcc gcaccgtatt gattgaagac ggcatcttgg tcggctatat
    27541 gcaggacgaa accaacgccc gcctgacggg tacgcaatcg accggcaacg gccgccgcca
    27601 aagctacgcc tccgtcccga tgccgcgtat gaccaacacc tttatggaaa acggcagcta
    27661 cgagccggaa gaaatcatcg cgtccatcga caagggcatt tacgccgtca acttcggcgg
    27721 cggacaggtg gacatcacca gcgggaaatt tgtcttcggc gcgtccgaag cgtggtgggt
    27781 ggaaggcggc aggctgcaat accccgtcaa aggcgcgacc attatcggca acggccccga
    27841 agtgctgaaa cacgtttcga tgataggcaa cgataccgcg ctcgacagcg gtgtcggcgt
    27901 gtgcggcaaa gaagggcaga gcgtccccgt cggcgtgggg cagccgaccc tgcgtatcga
    27961 tgccgggctg accgtcggcg gcagcgcaat ctgacgcgga atccggcacg atgccgtctg
    28021 aaaggttttc agacggcatc gccttattct tttttccgat gtttgatatt cctaagcatt
    28081 ctttaacatt atcttacaag ttcattaaaa taccgccttt cgttaagctg atttctgatg
    28141 aactggcctt atttaatcga tgccgtaccc aaattcgccg atgcggcaaa gctgacgctg
    28201 gaattgtccg tttacggcgt tgttttgtcg ttgctgttcg acctgcccgt cgccgtggta
    28261 acggcatacc gcatccgccc cttctacgca ttggcgcgcg cctatatcga gctgtcgcgc
    28321 aatacgcccc tgctgatcca attgtttttc ctatattacg gtctgccgaa aatgggcatc
    28381 aaatgggacg gtttcacctg cggcgtaatc gcgctggttt tcttgggcgc aagctatatg
    28441 gcggaagctg tccgcgccgg catcctcgcc gtccccaaag ggcaggtcgg agcgggcaag
    28501 gcaatcggtt tgagccgctt tcaagtgttc cgctatgtcg aattgccgca ggtttgggcg
    28561 gtcgccgttc ccgccatcgg cgcaaacata ttgtttttaa tgaaagaaac atccgtcgtc
    28621 agcacggtcg gcattgcgga attgttattt gttaccaaag acgtcatcgg tatggactac
    28681 aaaaccaatg aagccttgtt cctgctgttt gccgcttatt tgattatcct gctgcctgtt
    28741 tctttgttgg cgcggcggat tgaaaaccgc gtacggagtg cgaaatatgg cgtttgaatg
    28801 gctgtttgaa ggaaaaaatg cggcacggtt gggcgaaggc ctgctcttaa cggcacaaat
    28861 ttctttaatc tctgttgcgg cttcttgcgt attgggcacg ctgttcggct tggttttgcg
    28921 ttcgcgcaac cggcttgtcc gctttgtcgg acggttttat ctcgaaacca tccgaatcgt
    28981 gccgattttg gtgtggctgt tcggcctgta tttcggccta tccgtctgga caggcatcca
    29041 tatcggcgga ttttgggtct gcgtctgggt attttccctg tggggcgttg ccgaaatggg
    29101 cgacttggtg cgcggcgcac tggaatcgat tgaaaaacac caggtcgaat cgggtcttgc
    29161 gcccggcttg agccgggggc aggtattccg ctgcatcgag ctgccgcaaa gcatccgccg
    29221 cgtattgccc ggcgcggtca acctgttcac gcgcatgatc aaaaccagct cgctcgcctg
    29281 gcttatcggc gtgatcgaag tcgtcaaagt cgggcagcaa attattgaaa actcgttgct
    29341 gacgcagccc aatgcttcat tttgggttta cggcctgatt tttatgctgt atttcttctg
    29401 ttgctggccg ctatctctac tggcggcaaa acttgaacaa aaatgggaac actgacatgg
    29461 ctttactgag catccgcaag ctgcacaaac aatacggcag cgtaaccgcc atccaatcct
    29521 tagacttgga cttggaaaaa ggcgaagtca tcgtactgct gggcccgtcc ggctgcggca
    29581 aatccaccct cctgcgctgc gtcaacggtt tggagccgca ccaaggcggc agcatcgtga
    29641 tggacggtgt cggcgaattc ggcaaagacg tttcctggca aaccgcccgg caaaaagtcg
    29701 gtatggtctt tcaaagctac gaactgtttg cccacatgac cgtcatcgaa aacatcctct
    29761 taggcccggt aaaggtacaa aaccgcgacc gtgccgaagc agaggcgcaa gccggcaaac
    29821 tgttggaacg cgtcggactg ctagaccgca aaaacgccta tccgcgcgaa ctttccggcg
    29881 gtcagaaaca gcgcatcgcc attgtccgcg ccctgtgcct gaatccggaa gtcatcctgc
    29941 tggacgaaat caccgccgca cttgaccccg aaatggtgcg cgaagtcttg gaagtggttt
    30001 tggaactcgc ccgcgaaggg atgagtatgc tcatcgtaac ccacgaaatg gggttcgcac
    30061 gcaaagttgc cgaccgcatc gtctttatgg acaaaggcgg catcgtcgaa tcgtccgacc
    30121 ccgaaacctt tttttccgca ccaaaaagcg aacgcgcccg ccaatttctg gcaggtatgg
    30181 actactgatt taccgcaaac cgtccgaacc cttttcagac ggcatttcat agggcagtgc
    30241 tttatgtgtc gtcctgtgtg ttgaaacatc ccaagcaaac attcaaaaag gagatttcca
    30301 atgaaattga acgccaaact caaagccctt ttggcttccg ccgccatcgc cgtcggtctg
    30361 accgcctgcg ggggcggctc cggcgatgcc caatcttcac aaagcagcgg tgcggcaacc
    30421 gttgccgcca tcaaagaaaa aggcgttatc cgcatcggcg tattcggcga caaacccccg
    30481 ttcggctatg ttgacgcaaa cggcaaaaac caaggctttg acgttgaaat cgccaaagac
    30541 ctggccaaag acctgctcgg cagccccgac aaagtcgaat tcgtcctgac cgaggccgca
    30601 aaccgcgtcg aatacgtccg ttccggcaaa gtcgacctca tcctcgccaa ctttacccaa
    30661 acccccgaac gcgccgaagc cgtcgatttc gccgatcctt acatgaaagt ggcgttgggc
    30721 gtggtttccc ccaaaaacaa accgattacc gacatggcgc aattgaaaga ccaaaccctg
    30781 ctggtcaaca aaggcaccac cgccgacgct ttcttcacca aaagccatcc cgaagtcaag
    30841 ctgttgaaat tcgaccaaaa cactgaaacc ttcgacgcgc tgaaagacgg tcgcggcgta
    30901 gcactcgcgc acgacaacgc cctgctgtgg gcgtgggcga aagaaaaccc gaactttgaa
    30961 gtcgccatcg gcaatctcgg tcctgccgaa tttatcgccc ccgccgttca aaaaggcaac
    31021 gccgacctcc tgaactgggt caacggcgaa atcgccgcca tgaaaaaaga cggccgtctg
    31081 aaagccgcct atgaaaaaac cctcttgccc gtgtatggcg agaaagtcaa accggaagca
    31141 ttgttggccg aataagtttg tttgacaaga aaatccgtat ccgcatttga aggcgggtgc
    31201 ggattttttt tcagccgccg aaaatattgc aaattcaaag gaaaaatcaa ctatctcatc
    31261 gctttattta ttttattatt cttgcgcgtg cggaaatcaa gttcgtccgg ttttgcctgt
    31321 ttttgaattt cgggaaactt ccaaatcgtc attccagata aaacgcttca gccttatgtt
    31381 tccggatgac cgttttcgcg ggatgacggc ggagcgtttc tgttattcgg ataaattcct
    31441 acggtattga aacggcgata aggtgttttc ctttattata taatttcaat gcaaaatgat
    31501 attttttgaa caagcgtgcc gcaaagccgg aaaaaatgtt caagccgcat cggcggcaga
    31561 cgggacgaaa aatgacgttt gacgaatggt tgggcttgtc aaaactgcct aaaattgaag
    31621 caaggatgct gctgcaatat gtttcggaat atacgcgcgt gcagttgttg acgcggggcg
    31681 gggaagaaat gccggacgaa atccgacagc gggcggacag gctggcgcaa cgccgtctga
    31741 acggcgagcc ggttgcctat attttaggtg tgcgcgaatt ttatggcaga cgctttacag
    31801 tcaacccgaa cgtgctgatt ccgcgccccg aaaccgaaca cttggtcgaa gccgtattgg
    31861 cgcgcctgcc cgaaaacggg cgcgtgtggg atttggggac gggcagcggc gcagttgccg
    31921 taaccgtcgc gctcgaacgc cccgatgcgt ttgtgcgcgc atccgacatc agcacgcccg
    31981 cccttgaaac ggcgcggaaa aatgcggcgg atttgggcgc gcgggtcgaa tttgcacacg
    32041 gttcgtggtt cgacaccgat atgccgtctg aaaggcaatg ggacatcatc gtgtccaacc
    32101 cgccctatat cgaaaacggc gataaacatt tgtcgcaagg cgatttgcgg tttgagccgc
    32161 aaatcgcgct gaccgacttt tcagacggcc taagctgcat ccgcaccttg gcgcaaggcg
    32221 cgcccgaccg tttggcggaa ggcggttttt tgttgttgga acacggtttc gatcagggcg
    32281 cggcggtgcg cggcgtgttg gcggagaatg gtttttcggg agtggaaatc ctgccggatt
    32341 tggccggttt ggacagggtt acgctgggga agtatatgaa gcatttgaaa taattgtttg
    32401 caaaattggc ggattttccg ttattgggtt atgatgtgtt ttgatatgta aacatttata
    32461 ggtttggagc gataaacaat gaatgcagtc gtagttgccg taatcgttat gctggtgctg
    32521 tcgctgtcgc gcgtgcacgt ggtattgagc ctgacggtcg gcgcgtttgt cggcggcgcg
    32581 gtggcgggta tgccgctgca aaacattgcc gatgcggcgg gacaggtcag tcaggcgggg
    32641 attatccccg tgttcaacaa aggtttggaa ggcggcgcga agattgcgct ttcttatgcg
    32701 atgcttggtg cgtttgcgat ggcgattacc cattccggcc tgccgcagca gcttgccggc
    32761 gcggtcgtcc gcaagctgaa ccggggcggt atgcccgaca gcgtgcgttc gggcgagggc
    32821 gcggtcaaat ggctgctgct ttccatcatc cttgtgatgg gcattatgag tcagaacgtc
    32881 atacccatcc acatcgcctt tatcccgatg attgttccgc cgctgctttt ggtgtttaac
    32941 cgcctgaaaa tcgaccgccg cctgattgcg tgcgtcatca ctttcgggct ggttacgact
    33001 tatatgttcc tgccttacgg cttcggcgcg atttttttga acgaaatcct gttgggcaac
    33061 atccattccg ccgcgccgca gcttgatgtg aaaaacatca acgtgatggc ggcaatggcg
    33121 attcccgcat tgggaatgct ggccggactc ctgctggcgt ttgtccacta ccgcaaaccg
    33181 cgactgtacc aaagcaacaa tgccgatacg gcgggcaacg ccgatgcggc aaaccgtccg
    33241 cagccgtccg cctaccgcag cctggttgcc gccgccgcca tcgccgtatg ctttgccata
    33301 cagttgatgt atgaagattc gctggtgttg ggcgcgatgc tcggtttcgc cgtatttatg
    33361 atgttggggg tcattaaccg cgacaaggca aacgacgtat tcggcgaagg catcaagatg
    33421 atggcgatgg tcggcttcat tatgattgcc gcgcagggtt ttgccgccgt gatgaatgcg
    33481 accgggcata ttcagccgct ggtggaaagc agtatggcga tattcggcaa cagcaaaggt
    33541 atggcggcgt tggcgatgct ggtggtgggg cttttggtaa cgatgggcat cggttcgtcc
    33601 ttttccactt tgccgattat tgccgcgatt tatgtgccgc tgtgtaccgg tttgggcttt
    33661 tcgccgcttg ccaccgccgc cattgtcggc acggcggggg cgttgggcga tgccggttcg
    33721 cccgcgtccg attccacgct gggcccgacg atggggctga acgccgacgg gcggcacgac
    33781 catatccgcg attccgtcat cccgaccttc atccactaca acatcccgct tctgattgcc
    33841 ggctggattg ccgcgatggt gctgtaaatg gacgcggttc aagagttgga acgccgtatt
    33901 gtcgaactgg aaatccaaac cgcgcttcag gaggacgtaa tctccggtct gaacgcgatg
    33961 gtggcggaat tgcggcagac gttggatttg cagcaggctc agttgaggct gctgtatcaa
    34021 aaaatgcagg acaggaatcc cgacgcgcaa gagccgtatt ccctgcgcga cgagattccg
    34081 ccgcattatt gatgcgccgc cgtatccgga tttccttaaa taaaggctgt gtttgaatat
    34141 tccgccggtg caaccctaag atgtaacctt aatcagtcgg acaaaatgcc ttttatccga
    34201 tgaaaccggt tcttgtccga acggcgatga gcggcttccc ccgctgcaaa cagataaaac
    34261 caaccgggta ttcaaacaca gccaaaaaaa gccgtccgaa cccaaagggg acagacggcc
    34321 aaacacatta cggggaaaac gtcttactca atgagtctgc gaaacagaca ttgctaaaac
    34381 aactgcaatt ataaggtaag tttatttttt tgcaaagaaa tgtaaatttt taaattttta
    34441 aaaccgttcc gccgcatccg ttcggatgcc gtctgaaacg gcggcagggt tttaccgtgc
    34501 cgcgccgcaa accgggcatt cggggttgcg cggcaggtcg aaatattgcc agcccccttc
    34561 caaggcacgg taaaccgcca gcctgccgtg cgacggttcg cccgcttcca gcaggatttt
    34621 cagagcctcc gccgcctgtg tgcagccgac gatgccgacc agcggcgaga gcacgccgaa
    34681 gagggaacag atgccgtctg aagccgatcc gccgtcgaac aggcaggcgt aacacggcga
    34741 gtcgggcaag tcgggacggt atgcggcaag ctgtccttca aaacgtaccg ccgcccctga
    34801 aaccagcggt gttttcgttt gcacgcaggc acggttgacc gcctgccgcg tggcgtagtt
    34861 gtcgcagcag tctaaaacga tgtcggcggt ttgaaccaaa ccggtcaggc ggcagccgtc
    34921 gagtttttcg ttgacggcgc ggacatcgac agtatggttg atgcgtttca ggcggcctgc
    34981 caaggtttcg gctttaggct tgccgacatc gccctcgtca aatgcgactt ggcgttgcag
    35041 gttgtgcagc tcgaccgtgt cggaatcggc tatggtcagc gtgccgacgc ccgaggcggc
    35101 gagatagggc agggcggcgg cgcccaatcc gccgcagccg acgaccaaaa tatgcgcggc
    35161 ggaaagtttc tgctgccctt cgatgccgat ttcgtccaag aggatgtggc ggctgtaccg
    35221 cagcaggagt gcgtcgtcgt tgtcgtgttc ggtcgcggtc atgatgatgt tcggaaaaaa
    35281 cagttgcggg cgattgtaac gccgccgtcg ggcggcgttc aacttcagac ggcatttcgg
    35341 gacatgagtg gttaaagtgt gaacggtttg gcacggatgc ggcatttgag gtacatttac
    35401 agtatttacg gcaatgagag gaaaatcatg caactgcata tcctgaacaa tccgaaggac
    35461 gcggctttgg cggcggacgc ggaatttttg aaacagtccc tgttcaacct cctgcacgaa
    35521 gaagcctcgc cgttggttgt cgaaacggtc aaactcttgt ccacttccga cgacagcgcg
    35581 gcattgattg aaaaagtatt gccgcaattg gacgaacggc agacctacga tctgacgctt
    35641 gcctgcgggc tgttcgccca gattctgaat atcgccgaag acgtgcacca cgaacgccgc
    35701 cgccaaatcc acgaagatgc cggccacaac gcagcagaag gcagcctgac ggaaaccgtc
    35761 cgcaggctca aagcggggaa agccgacggc aaatcggtgc agcggcagtt ggacaatacg
    35821 tccgttaccg ccgttttgac cgcgcacccg accgaagtgc aacgccaaac cgttttgaat
    35881 ttcaaccgcc gcatccgcgc gctgctgccg caacgcgaac gctgcaccaa tgccgacgcg
    35941 ctggcacggc tgcgccgcga aatcgacacc gtgcttctgg gcttgtggca gaccagcgaa
    36001 acgcgccgcc acaaactcag cgtcaacgac gaaatcaaca acggcgtgtc catcttcccg
    36061 atgagctttt ttgaagccct gcccaaactc taccgcaaga tggaacacga ctttcagacg
    36121 gcctatcccg atgtccgcgt tccgaacatc cttaaaatcg gcggctggat cggcggcgac
    36181 cgcgacggca atccgtttgt ttctggcgaa accctgcgct ttgccttccg ccgccacgcc
    36241 gatgccgtgt tccgttttta ccgcagcgaa ctcgacaaac tttaccgcga actgccgctt
    36301 tccatccgcc gcgtcaaagt caacgacgac gtaatggcac tggcagccct ctcgcctgac
    36361 gaagaaatcg cccgcaccga agaaccttac cgccgcgcca ttgcctacat tatggcgcgc
    36421 gctatgggca aagcgcgctc gctcggtttg ggcatgggct gcaaattcgg ctttctcgag
    36481 ccttatgctt cggcccaaaa atttctcgat gatttgaaaa aattgcaacg ttcccttatc
    36541 gacaacggca gccgcctgct tgccgaaggc cgtttggcag acctcatccg ttccgtgtcc
    36601 gtgttcggct tccacatgat gccgctcgac ctgcgccaac acgcaggcaa acacgccgat
    36661 gtggttgccg agcttttcca acacgcaggc ttggaagact acaacagcct gaacgaagag
    36721 caaaaacaag ccgccttgtt gcgcgaattg ggccaccaac gtccgctgta cagcccgttc
    36781 atcacataca gcgaccatac ccgccgcgaa ttggcgattt tcaacgaagc gcgcaaaatc
    36841 aaagacgaat ttggcgaaga tgccgtaaca caaagcatta tttccaactg cgaacaaccc
    36901 ggcgacctgc tcgccttggc attgctgctg aaagaaagcg gcctgttggc ggtggaaaac
    36961 ggcaaaccgc acagccgcat caacatcgtg cctctgtttg aaaccatcga agcgctcgaa
    37021 aatgcctgtc cggtcatgga aaccatgttc cgcctcgact ggtatgatgc cctgctcgaa
    37081 agccgcggca acatccaaga aatcatgctc ggctattccg actccaacaa agacggcggc
    37141 tacgttacca gctcatggtg tctgcatcag gcggaattgg gcttggtcga actcttcaaa
    37201 aaatacgatg tccgcatgcg cttgttccac ggtcgaggcg gcagcgtcgg acgcggcggc
    37261 ggcccgtctt accaagccat cttggcgcaa cccgcaggca gcgttgccgg acaaatccgc
    37321 attaccgagc aaggcgaagt catcaccgcc aaatacgccg accccggcaa cgcccaacgc
    37381 aacttggaaa ccttggttgc cgcgactttg gaagccagca tcctgccgga taaaaaagac
    37441 cctgatgcca aactgatgca ggcattgtcg gacgtatcgt tcaaatacta ccgcgaactg
    37501 attacccacc ccgacttcat cgactacttc ctgcaaacca gcccgattca ggaaatcgcc
    37561 accctcaacc tcggcagccg tcccgccagc cgcaaaaccc tggcgcgaat tcaggattta
    37621 cgcgcgattc cgtgggtatt ctcctggatg caaaaccgcc tcatgctgcc ggcttggtac
    37681 ggtttcggca gcgcggtgga aaccttgtgc gaaggcagcc ccgaaaccct cgccgccctg
    37741 cgcggacacg cccaaaacaa ccccttcttc caagcgatgc tctccaatat ggaacaagtg
    37801 atggcgaaaa ccgacatcac cttggcggaa aactacgccg gtttgagcga atcacccgaa
    37861 aaggcaaaag tcatcttcgg catgattaag gaagaatacc gacgcagccg caaagccctg
    37921 ctcgacctgc tgcaaaccga agaacttttg cgcgacaacc gcagcctcgc ccgttcgctc
    37981 gctttgagga ttccatacct gaacgcgctc aacggtttgc aagtcgccat gctcaaacgc
    38041 ctgcgcaaag aacccgacaa tccgcacgcc ctgctgatgg tgcacctgac catcaacggc
    38101 gtggcgcaag gtttgcgcaa taccggctga tagtgccgca tcggggcaaa atgccgtctg
    38161 aacgcctttc agacggcatt tacctgaccg cacttgcaga gaaacaccga ttgttttaaa
    38221 gtgaacggca gtgatatgtt gaaagacgac caatgaaaat taccgttatc ggcgcaggtt
    38281 cgtggggtac ggcgctcgcc ctgcattttt cccaacacgg caaccgcgta tccctgtgga
    38341 cgcgcaacgc agaccaagtc cgccaaatgc aggaagcgcg tgaaaacaaa cgcggactgc
    38401 ccggcttttc ctttcccgaa accttggaag tgtgtgcgga tttggcagag gcgctcaaag
    38461 acagcggact tgtccttatc gtaacctccg ttgccggatt gagaagcagc gcagagctgc
    38521 tcaaacagta cggcgcggga cacctccccg tcctcgccgc ctgcaaaggc ttcgagcagg
    38581 atacagggct gctgaccttc caagtcttga aagaagtatt gcccgacaat aagaaaatcg
    38641 gcgtactttc cggtccgagt tttgcacagg aactcgccaa acaactgccc tgcgccgtcg
    38701 tcctcgcctc cgaaaaccaa gagtggattg aagaactcgt accgcagctc aacacgaccg
    38761 tcatgaggct ttacggcagt accgacgtta tcggcgtggc agtaggcggt tcagtcaaaa
    38821 acgtcatggc aatcgctacc ggactctccg acggactcga atacggactc aacgcccgtg
    38881 ccgcactggt tacgcgcgga ctggccgaaa tcacccgcct tgcctccgca atgggcgcgc
    38941 agcccaaaac catgatgggg cttgccggca tcggcgatct catcttgacc tgtaccggtg
    39001 cactttcgcg caaccgccgc gtcggcttgg gtttggcaga aggcaaggaa ctgcatcagg
    39061 tgctggtcga aatcgggcac gtttccgaag gggtcagcac catagaagaa gtcttcaata
    39121 ccgcctgcaa ataccaaatc gatatgccga ttacccaaac cctgctgcaa ctcatccgca
    39181 aagaaatgac ccctcagcag gtcgtcgaaa ggctgatgga acgcagcgcg cgtttcgaat
    39241 aaacaacaaa cagatgccgt ctgaaccccc agacggcata cagacaggta aggtcatgaa
    39301 acaaaatatc gaaaaactcg aaagcagcgt ttatacgttg gtacaaaaat tcgaaaccct
    39361 cgtcagcgaa aaccgccgcc tcaaagaaac cgtcgccgaa ctcgaacggg cgcacgagcg
    39421 gcaaaaactc gaacacgaga ccgccgtcga cgaactcagc gaagccctgc tcgtccaagt
    39481 cggcaaactc aaagaagacc tgcaaaacaa aatcgacagc ctgacggaag aaaatgcacg
    39541 ataccgcgcc ctgctcgaac agagcaggga gaaaatcagc gcactggcag cgcgcctccc
    39601 ccaacggcag gaaacgcagc aataaggatt aaaggatgaa catcgaacaa gtctacatcg
    39661 aagtcatgca cgcccggctg accgtcaaca cgccggcaga agaaaaagac acactgttgc
    39721 aggcagtcgg aatgctcaac ggcaaagccg aagccatccg cgaaggcggc cgcgtcgcgg
    39781 acagcgaaaa aatcgtcatt atggccgcgc tcaacgtcgt ccacgaccta ttgaaaacct
    39841 ccctgaacgg cggcgatttg gcaatcggcg attttgcgcg taaaataacc gatatggaca
    39901 acgcctgcca aaaggcacta tcccgcttgg ggcaggaata accccttttt ccctgcggtg
    39961 tccgcgaagg catatattcc tttgaaccaa taagttcgct taaggttgcc gggagcagta
    40021 gtgggcgcga gcgtcctttt gcggacgcac ccgaaactac ccggggcagc cgccttgtca
    40081 gcaaggttca agcggattcg gccgaaacgg ctctcgcggg gattcccatt caaaaccgca
    40141 tcgcaacgat gcggtttttc acattggccg ccgccccgcc cgaaaaagca gtatgcagtc
    40201 tgccgaaatc cggaaaatcc ttccgtaacg cggtaatgcg attgacaaaa tcaaatcctg
    40261 tctttaaaat ccataacttt ctatatctat ctggatttcc actatgaaaa ccttttcagc
    40321 gaaaccccac gaggtgaagc gcgaatggtt cgtcatcgat gcccaagaca aagtcttggg
    40381 tcgcgttgca accgaagtcg ccagccgtct gcgtggcaaa cacaaacctg aatacacccc
    40441 ccacgtcgat accggcgatt acatcatcgt catcaatgcg gacaaactgc gtgtaaccgg
    40501 tgccaaattc gaagataaaa aatacttccg ccattccggt tttccaggcg gcatctacga
    40561 gcgcactttc cgcgaaatgc aagatcaatt cccggaccgc gctttggagc aggctgtaaa
    40621 aggtatgctg cccaaaggtc cgctgggtta cgccatgatt aaaaaactga aagtgtacgc
    40681 tggtgcggag catgcccatg ctgcgcaaca acccaaagtt ttggaactga aataataagg
    40741 acacgacatg aacggtaaat actactacgg cacaggccgc cgcaaaagtt cagtggctcg
    40801 tgtattcctg actaaaggta cgggtcaaat catcgtaaac ggccgtcccg ttgacgaatt
    40861 cttcgcacgc gaaaccagcc gtatggttgt ccgccaacct ctggttctga ctgaaaacgc
    40921 cgaatctctc gacatcaaag taaatgttgt cggcggcggc gaaaccggcc agtccggcgc
    40981 aatccgccac ggtattaccc gtgccctgat tgattttgac gctgctttga aacctgcctt
    41041 gtctcaagcc ggtttcgtta cccgcgatgc gcgcgaagtc gaacgtaaaa aaccgggtct
    41101 gcgcaaagca cgccgtgcaa aacaattctc caaacgttga ttttggaaat tcaaaaaacc
    41161 ctgcttatcg cagggttttt tatttgtaac aggcggtttc ccattggtaa tctaaagatt
    41221 acggattggg caaaaatcaa aaacagcacc agtatcgact acacggacga actgaccgtc
    41281 agggatggct gttactggag aggacatcgt atttctactt tgtaaaataa cttttaattt
    41341 atttagagtt attgaaaaaa tagaacggct atttcaacta gcctataaaa atggcaatct
    41401 attagggtta gcataggtgt taccgccgcc gttcaagccg atcagtaaag gaacagtttc
    41461 ctagttttta gctaacctgt attatataat ggggagaata tcttaaataa ccaagcatcc
    41521 ttaatcataa tttagagaaa ttattttttc agagtctaac ccttttaata tgattaactt
    41581 attaacttta tctatttccc agtctgatat tatatcggta gatactgaat tctctcttat
    41641 taaattttta tctgtaataa ttgcacccaa ctcatgaata atacaaattt tttgattatc
    41701 caataaataa atatcaaaaa ctattccatc tatatttagt atgttaactt ttgaattttg
    41761 tatagaaata caaaaaacga ctgaatcaaa tccgataaaa atatttttat catcaagcat
    41821 taatactttt gggggaatac ttatgcaaga aacagctaat ccaataccta tattgaattc
    41881 tttataaaaa gaaagaataa aacatcgata aatctctttt cctccctctt gtagaataat
    41941 atatttttga taggtgttta ttaaatagtc ttctaactct cttatggaat tataaggata
    42001 tttcgttaat ctcattattt tgttccatgt ctaaaacgtc ctgttgatcc atatatttca
    42061 cccgttatga gtctgttccc atctcatata gtttcaagtt taacaacccc ttgaggctat
    42121 gttaaaagag tttttctaat aactattccg ttttcttaaa tataaaaaaa tagaaaaata
    42181 gcccgggtat agtttgaaca agggaaatca gaccggttcc aactcactta atccgggcaa
    42241 gtccgcgaag ccgcgttcac gtatgatttg gcaaaagttg ttcagataac ttttatctgt
    42301 gtcttcagtg cggatggcgg catacagttt gctttgcagt ccgtcggcag taatttggcg
    42361 gtggacgaca tagccttttt caaggtaggg catgactgtc caatagggaa gggcggcaat
    42421 gccacgtctg ctggcaacca gttggataat ggcgatggtc agctcgctgt gccggcgcgg
    42481 cgggttgatg tttttcggaa tcaggatttt tttgggtaaa tccagcattt catcgggaac
    42541 gggataggta atcagggttt ccccgataaa gtcttccgcc gtccaaacgt ttttggcggc
    42601 aagcggatgg tccggggcgc aaatgccgac catttcgtag gcaaacagtg ggtggaagct
    42661 gatgctgctt tgtttttcgg cttcggagac aatggcgagg tcggcgcggt gttgcagcag
    42721 cagcccgacg ggatccgctt ggaatcctga tacgatatcc agttcgactt ggggccacat
    42781 cgggcggaat tcgcccatgg cgggcatcag ccagtcgaaa caggtatggc attcgacggc
    42841 aatccgcagc tctcccgcct ccccttccgt gattcgcgcc aaatctcgtt ctgcaacagc
    42901 aacttgaggt atcagttcgt gggcgaggcg cagcagcctt tcgcccaccg gggtaaagcg
    42961 caggggcgtg gatttgcgtt cgaacagcgg cgtgccgtag tggttttcga gcatacggat
    43021 ctggtgggaa agggcggatt gggtaaggaa aacccgtttg gcggcaaggg agacgctgcc
    43081 ggtttcttca agtgccagca gggttttgag gtggcgcaat tcgataatgg aatccatggg
    43141 gcttcagacg gcatattgaa ccggcgcata ttaaaataat tcatatgcgg gtgcaaatcg
    43201 gaaaaatggg cgtttcgggc atttgcggtt agaatgcccg cttgtttgga aatgacagga
    43261 aaaatatggt tttgcgcaga gattttttag cctggtgcga cgaaacgttg cagacggcat
    43321 cgtttaaaga ttacgccccc aatggtttgc aggttgaagg gagggaatat atcgggaaaa
    43381 tcgttacgtc ggtaacggca agcagggcag cgattgattt tgctgtggag cagaaggcag
    43441 atttgctttt ggtacatcac ggtatgttct ggaaaaacga gctgccgacc gttaccggtt
    43501 ggaaaaaaga acggattgcc gcactgttac ggcacgacat caatatggca ggctaccatc
    43561 tgcccctgga tgtgcatccc atactgggca acaatgccca actcgccgac agattgggtt
    43621 ttgcgacaga aaaacggttc ggcgaacaaa acctgctcaa ctcgggcagc ctgaaacaag
    43681 ccaagacact cggcgcattg gcggcgcata ttgaaacagt tttgcaacgt aagcctgtcg
    43741 ccatcggcaa tcccgaacgc gaaatccgac gggttgcatg gtgtaccggc ggtgcacagg
    43801 gcttttttca gacggcaata gatgaaggcg tcgatctgta tctgacgggt gaaatttccg
    43861 aagcccaata ccacctcgcc aatgaaacgg gtacggcttt catttcggca gggcatcacg
    43921 cgacggaacg ttacggcgta cgcgcgctgg cagaatcggc ggcagaggtt ttcgggttgg
    43981 aagtgtgcca ttttgacgaa aacaacccgg cttgagtctt gagaaatatc ataaaacttt
    44041 accttatttt aaataatgct ttgagtatca ctgcaaactg ggtaaaattg caatgtttaa
    44101 tggctcggca ttcccaaaat attaggacga ctgaataatg gataatcaag aaatcaacaa
    44161 cggccgccgc cgtttcctga cacttgcgac ctgcggcgca ggcggagtgg cagcattggg
    44221 tgtggcaacg ccgtttgtgg ccagtttttt cccttcggaa aaagccaagg ccgccggtgc
    44281 tgccgtcgaa gtggatgtca gtaaaatcga agcgggtcag ctgctgaccg ccgagtggca
    44341 aggcaaaccg atttgggtgc tcaaccgtac agatcagcag cttaaagacc tgaaaggcct
    44401 gaacggcgaa cttaccgatc ccaattccga tgcggaacag cagccggagt atgctaaaaa
    44461 cgagacccgt tcgattaagc cgaacatcct tgtcgccatc ggtatctgca cccatttggg
    44521 ctgctcgccc accttccgtc ccgacattgc ccccgccgat ttgggcgcag actggaaggg
    44581 cggcttcttc tgcccgtgcc acggttcgaa attcgacttg gccggccgcg tatataaagg
    44641 tgttcctgcc ccgaccaacc tggttgtccc gccatataaa tacttgagcg acacaactat
    44701 cttggtgggc gaagactaag aataaggaac gataattatg gcaaaccaaa ccaatagcaa
    44761 agcaaaagca ttgttaggct ggatggacgc tcgcttcccc ctgtctaaaa tgtggaatga
    44821 gcatttggcg caatactatg cgccgaaaaa ctttaatttt tggtactatt tcggctcttt
    44881 agccctgctc gtcctcgtga ttcaaatcgt cagcggtatt ttcctgacga tgaactacaa
    44941 accggacggc aacctgaatg cctaccatct gcctgctgcc tttaccgcag tagagtacat
    45001 catgcgcgac gtgtccggcg gttggattat ccgctacatg cattccaccg gcgcatcttt
    45061 cttcttcatt gttgtttatc tgcacatgtt ccgtggtttg atttacggtt catataaaaa
    45121 accgcgcgaa ttggtgtgga ttttcggttc tttgatcttc ttggcattga tggcggaagc
    45181 ctttatgggc tacctgctgc cttggggtca gatgtccttt tggggtgcgc aggtgattat
    45241 caacctgttc tccgccattc ccgtcatcgg tcctgatttg tccacttgga ttcgcggcga
    45301 cttcaacgtt tccgacgtta ccctgaaccg cttcttcgcc ctgcacgtta tcgccgtacc
    45361 gctggtattg ctcggtttag tcgtggcaca catcatcgca ctgcacgaag tgggctctaa
    45421 caaccccgac ggtgtggaaa tcaagaaaaa caaagatgaa aacggcattc cgcgcgacgg
    45481 catcccgttc cacccttact acactgttaa agacatcctg ggcgtcgttg tattcctgat
    45541 tgtcttctgt gccgtattgt tctttgcccc tgaaggcggc ggctacttcc tggaagcgcc
    45601 gaacttcgat gcggcaaatg cgctgaaaac acccccgcac attgcgccgg tatggtattt
    45661 cacaccgttc tacgccattt tgcgtgcgat tccttccttc ttgggaacgc aggtttgggg
    45721 tgtaatcggt atgggcgcgg cggtcgtgtt gattgccctg ctgccttggc tggacagggg
    45781 cgaggtgaaa tccgtccgtt accgcggtcc gattttcaaa accgcattgg tcttgttcat
    45841 catttccttt atcggtttgg gtattttggg cgcgatggtg gcaaccgata cgcgtacttt
    45901 ggtcgcgcgc atcctgtcct tcgtctattt cgcattcttc ctgggtatgc cgttctatac
    45961 caagctggat aaaaacaaac cggttcccga acgcgtaacc atgagcaccg ccaaacaaaa
    46021 aatcatgttc tttgtttacg tcggcattac cgccgtgggt gcttacctgt ttgcaaccaa
    46081 catctgatga gggcagtcaa aatgaaacaa gctatgaaaa attggtttgc tgccttgctg
    46141 tcggcagtac ccatgggtgc ggcatttgct tcgggcggtc atgcacacta tgagaaagtc
    46201 gatatcgatt tgcgcgacca agtcagcctg cagcgcggcg cacaaatctt taccaactac
    46261 tgtttgtcct gccactcggc aagcggtatg cgtttcaacc gtttgaaaga catcggtttg
    46321 actgacgaag aaatcaagaa aaacctgatg tttaccaccg ataatgtcgg cgatgtcatg
    46381 cattcggcga tgaacccgaa agatgcggca aaatggtttg gtgctgctcc gcccgatttg
    46441 acattgattg cgcgttccaa aggtgcagac tacctttacg cttatatgcg cggcttctat
    46501 aaagatccga cccgtccgag cggctggaac aatactgtat tcgataaagt cggtatgccc
    46561 cacccgttgt gggaacagca aggcgttcaa gccgttgagt tggatgccaa aggtcagccg
    46621 gttatggtaa aagacgaaca cggcgagatg aagcctaagc tgtattggga atctaccggt
    46681 ttgcacagcc gccgtctgcc taacggcaaa gtgatccaga aagagtacga cgcatatgta
    46741 cgcgatttgg tcaattacct tgtgtacatg ggcgaacctg cacaactgca acgcaaacgt
    46801 ataggctatg tcgtgatgat tttcctattt gcggttatgc tgcctttggc gtatttcctg
    46861 aaaaaagaat attggaaaga cgtacactaa gcgtttggaa caaaagggca aatcctttag
    46921 ggtttgccct tttttcattt tgcctgccgt ttgaaaagcc tgaatccgta tgccgtctga
    46981 acatagctgc aacatttcag acggcatcat tctaaaaatg tcaggcatcg ggaacttaat
    47041 cagggtttgt ggcagattga tattgaatta ggaaaaggtc tgaaaccgca agcagcatgg
    47101 taaggcgatg tcgaacaggt ttaaagccaa tccgctatat tttttggagc tgcaccgtgc
    47161 aaacaggtag aatggcggcg gttttgtccg gtgtgaaaat gtttgaatat taggatgatt
    47221 gatggagctg atgactgttt tgctgccttt ggcggcgttg gtgtcgggcg tgttgtttac
    47281 atggttgctg atgaagggca ggtttcaggg cgagtttgcc ggtttgaacg cgcaattggc
    47341 ggaaaaggcg gcaagatgtg attttattga acaggcacac gcagaaattg catcagaatt
    47401 ggccgctttg gatgggaaat acgagcattt gcaggacgaa aattatgctt tgggcaaccg
    47461 tttttccgca gccgaaaagc agattgccca tttgcaggaa aaagaggcgg aatcggttcg
    47521 gttgaagcag tcgtatatcg atttgcagga aaaggcgcag ggtttggcgg ttgaaaacga
    47581 acgtttggca acgcagctcg ggcaggaacg gaaggcgttt gccgagcaat atgcgctgga
    47641 acgccaaatc cgccaaagag tcgaaaccga tttggaagaa agccgccaaa ctgtccgcga
    47701 cgtgcaaaac gacctttccg atgtgggcaa ccgttttgcc gccgccgaaa aacagattgc
    47761 ctatttgcag gaaaaagagg cggaagcgga gcggttgagg cagtcgcata ccgagttgca
    47821 ggaaaaggcg cagggtttgg cggttgaaaa cgaacgtttg gcaacgcaaa tcgaacagga
    47881 acgccttgct tctgaagaaa agctgtcctt gctgggcgag gcgcgcaaaa gcttgagcga
    47941 tcagtttcaa aatcttgcca acacgatttt ggaagaaaaa agccgccgtt ttaccgagca
    48001 gaaccgcgag cagctccatc aggttttgaa cccgttgaac gaacgcatcc acggtttcgg
    48061 cgagttggtc aggcaaactt atgataaaga atcgcgcgag cggctgactt tggaaaacga
    48121 attgaaacgg ctccaagggt tgaatgcgca gctgcacagc gaggcaaagg ccctgaccaa
    48181 cgcgctgacc ggcacgcaga acaaggttca gggcaattgg ggcgagatga ttctggaaac
    48241 ggttttggaa aattccggcc ttcagaaagg gcgggaatat gtggttcagg cggcatccgt
    48301 ccgaaaagag gaagacggcg gcacgcgccg cctccagccc gacgttttgg tcaacctgcc
    48361 cgacaacaag cagattgtca ttgattccaa ggtttcgctg acggcttatg tgcgctacac
    48421 gcaggcggcg gatgcggatg aggcggcacg cgaactggcg gcgtatatcg ccagcatacg
    48481 cgcgcatatg aaaggcttgt cgctgaagga ttacaccgat ttggaaggtg tgaacacatt
    48541 ggatttcgtc tttatgttta tccctgtcga accggcctac ctgctggcgt tgcagaatga
    48601 cgcgggcttg ttccaagagt gtttcgacaa acggattatg ctggtcggcc ccagtacgct
    48661 gctggcgact ttgcggacgg tggcgaatat ttggcgcaac gagcagcaaa accaaaacgc
    48721 gctggcgatt gcggacgaag gcggcaagct gtacgacaag tttgtcggct tcgtacagac
    48781 gctcgaaagc gtcggcaaag gcatcgatca ggcgcaaaac agctttcaga cagcattcaa
    48841 gcaacttgcc gagggacgcg ggaatctggt cggacgcgcc gagaaactgc gtctgttggg
    48901 cgtgaaggca ggcaaacagc ttcaacggga tttggtcgag cgtgccaacg aacagacggc
    48961 atttgctttg ggcaaacctt cggaacagga agcggctgac gaagccgaat aggaaaggcg
    49021 gatgcgtttc agacggcatc agtgcagatg atgccgtctg aagactttgg gaagtcggtt
    49081 agaaaaacgg attgtgccgt ttttcgtgtc cgatggaagt catacgcccg tgtccggcga
    49141 caacttgcac ggtttcgggg aaggcgaata atttgttgcg gatattgttg attaagtcgg
    49201 cgtggttgcc gcgcggaaaa tcggtcctgc ctatggtttc gtaaaacagc acgtcgcccg
    49261 caatcagcag ctccgcttcg gcacaataga aaaccacgtg tccgggcgtg tgccccggaa
    49321 tatgcagcac ttggaaggca tagcgtccga ccgtgagcgt ttcgccttct tcgagccaac
    49381 ggttcggcgc aaaggcgggc gagacgggga atccgtattg cgcggtggtt tgcggcagcg
    49441 attggagcag gaattcatct tcgcggtgcg ggccgaggac ggggacttta tgcgtttcca
    49501 gcatctcgac cacgccgccc gcgtgatcga gatgaccgtg cgtcagccag attgccgtga
    49561 gcgtgagttt gcggtttgcc aacgcttgca gcaggaacgg cacgtcgccg ccgacatcgg
    49621 tcaggacggc ttcgccgctt tcgtcgtccc aaatcagggt gcagttttgg cggaaggggg
    49681 taacggggag aatttcgtaa cgtagggtca tcggcgtgtt cctaaaacgg tttttcagac
    49741 ggcatcgggt ttgccgtttg ttttatggcg gtttgccgcc tgttttgata ttgggggaat
    49801 caggtgatta agaagataat cggcggcatc ataccgattt ttacggcggt tttcatccct
    49861 gcatcggcag gcgcggcgga tttgatgctg gcgcaggaat acaaagggca ggacattgcc
    49921 ggctgggcga tgagcgagaa gctcgacggc gtgcgcgcct attgggacgg aaagcacctg
    49981 ataagccgtc agggctacgc gtttacgccc cccaaaggct ttaccgcgca gtttccgcct
    50041 tatcctttgg acggcgaatt gtatagcggg cgcggtcagt tcgagcagat ttccgctacc
    50101 gtgcgttctg tttcttcaga ctggcgcggc atccgcctgc acgttttcga cgtacccaag
    50161 gcgcagggca acctctacca acgtttggca gtcgcaacgc agtggctgaa aacgcatccg
    50221 aacgcgccga ttaccatcat cccgcaaatc aaagtgcgcg accggcggca cgcgatggac
    50281 tttttaaaac aaatcgaagc gcagggcggc gaaggcgtga tgctgcgcca gcccgaatcc
    50341 cgttacagcg gcggcaggag cagccaatta ttgaagctga aaagccaata cgacgacgaa
    50401 tgcacggtaa cgcggcacta tgagggcaaa gggcgaaacg ccggacggct gggcgcggtc
    50461 ggctgcaaaa accgacacgg cgaattccgc atcggcagcg gtttcaagga caaagaccgc
    50521 gacaacctgc ccaaaatcgg cacgctgatt acctaccgtt accgtggctt tacgcggaaa
    50581 ggcacgccga aatttgccac atttgtgcgc gtgcgtaccg accgctgagc aggacagttc
    50641 agacggcatc cgatactttg gtttataatt ttccttttac cgaccgattc cgacatatga
    50701 ccgatttaga aaccaaacgc cttgaaacac aggcgatgct tgaaaacgcc gatcttttgt
    50761 tcgaccaagg ccaatgccgt gccgcactgc aaaaagtggc gtacgagatt acgcgcgatt
    50821 tgggggataa atatccgttg ctcctgcccg tgatgggcgg ggcggtggtg tttacggggc
    50881 agttgctgcc gctgttgcgt tttcccttag attttgatta tgttcacgtt tcccgttacg
    50941 gcgacaagct ggagggcggc gcgttcaact ggaagcgtat gcccgatgcg gaacaaatcc
    51001 ggggcaggca cgtcgtcgtg ctggacgata ttttggacga agggcatacg atgtccgcca
    51061 ttcaagccaa acttttggaa atgggtgcgg caagctgccg tgcggcggtg ttcgccaaca
    51121 agctgattga caaacccaag cctatccgag ccgattatgt cggactggat gtgccgaacc
    51181 gttatgtttt cggttacggc atggatgcgg cgggctgctg gcgtaatttg ggcgagattt
    51241 acgcattgga cggaaaataa ggacgcgatg ccgtctgaag gcctttcaga cggcatcgcg
    51301 cccatacgcc ggcaggatta atgaggaaca ggacgcatca atatgatagg gcttttaatc
    51361 atcacacacg aaaccatagg cgaagcctac cgcaagctgg cgcatcattt ttttccgggc
    51421 gggctgcctg aaaacgtccg catactcggc gtgcagccga cggaaggcca agacgacatt
    51481 atcaacaacg ccatttccgc gctgcaagag tttcctgaaa accacggtgt attgattatg
    51541 accgacatct tcggcgcgac cccctgcaat gccgcccgcc ggctcgtgcg cgaaaacaaa
    51601 tcggcgattt tgaccgggct gaacgcgccg atgatgatta aggccgtcca atattcgccg
    51661 gcggcggaag actttgccgc ctttaccgaa tgcgtcaggg aggcggcggt aaaaggcatt
    51721 ttcgccatca cgtccgcgcc cgaagatttg gtgtgccggc gcagcggcga tgccgtctga
    51781 agaagcggca gggcaggaaa acattttaaa cggcgtctgc tgccgatata tagcggatta
    51841 acaaaaatca ggacaaggcg gcgggccgca gacagtacaa atggtacgga accggttcgc
    51901 ctggtgcttc agcaccttag ggaatcgttc cctttgagcc aaggcggggc aacgccgtac
    51961 tggtttttgt taatccacta tacataacac gggaaccgaa atgctcaaac aatccatcga
    52021 aatcatcaac aaactcggac tccacgcccg cgcgtccagc aagttcaccc aaaccgcgtc
    52081 ccaattcaaa agcgaagtct gggttacgaa aaacggcagc cgcgtcaacg gcaaaagcat
    52141 tatggggctg atgatgctcg cagccgccaa gggtacggtc atcgaactgg aaaccgacgg
    52201 cttggacgaa gccgcagcca tgaaggcatt gaccgactta atcaacgact acttcggcga
    52261 gggcgaataa tgagtatcgt gctgcacggc gtggcggcgg gcaaaggcat tgccgtcggt
    52321 tgcgcccacc tgattgcgcg cggtacggag gaagtgccgc agtatgatgt tgctcaggcg
    52381 gacaccgatg ccgaagccga acgtttcgat gccgccgtca aagccacgcg caaagagttg
    52441 gaacagctcc gcagcgcgat tcccgaaaac gccccgaccg agttgggcgc gttcatctcg
    52501 ctgcacctga tgctcttgac cgatgttacc ttgtcgcgcg aacccgtcga tattttaagg
    52561 gaacaaaaaa tcaacgccga gtgggcattg aagcagcaga gcgacaaact cgccgcccaa
    52621 ttcgacaata tggacgatgc ctatttgcgc gaacgcaagc aggatatgct gcaagtcgtc
    52681 cgccgcatcc acaacaacct gatcgggcag ggcaacgagt tggaggttgc cgacaacctg
    52741 tttgacgaaa ccgttctgat tgcaaacgac ctttcacccg ccgacacggt tttgtttaaa
    52801 gagcagcgca ttgccgcctt cgttaccgat gccggcggcc ccaccgggca tacggcgatt
    52861 ttgggcagga gcttggacat cccgtccgtc gtcgggctgc acaacgcgcg caaactgatt
    52921 accgaaggcg aaacggtcat cgtggacggc atcaacggcg tgttgattat ttcgccggat
    52981 gagtcggtgt tgaacgaata ccgccgccgc gcccgcgaat accgcagcca caaacgcgat
    53041 ttgaacaagc tcaaaaaaac cgccgccgcc accgccgacg gggtctgcat cgagcttgtg
    53101 ggcaatatag aatccgccga agacgtgaaa ccgctgcaca acctcggcgc agacggcatc
    53161 gggctgttcc gcagcgagtt tctttacctg aaccgcgata cgatgccgtc tgaagacgag
    53221 cagtacgaag tgtacagcgc gattgtcaaa aaaatgaagg gcaaaagcgt aacgatacgg
    53281 acagtcgatt tgggtgtgga caaaaatccg cgctggttcg ggaaaaacag cacgcccaac
    53341 ggcagcctca accccgcgct gggcatgacc ggcatccgcc tgtgccttgc cgaaccggtc
    53401 atgttccgca cgcagatgcg cgccatcctc cgtgcggcgg tacacggtcc cgtccgcatg
    53461 atgtggccga tgattacctc cgtatccgaa gtgcgccagt gcctcatcca cctcgacacc
    53521 gcgcaacgcc agcttgccga acgcggcgat gccttcggcg aagtcggcat cggctgcatg
    53581 attgaaattc cgtctgccgc gctgaccgtc ggcagtattt tgaaactggt cgatttcatc
    53641 tccgtcggta ccaacgattt gatccaatat attctgtccg tcgatcgcgg cgacgacagc
    53701 gtcagccatc tctaccaacc cggacatcct tccgtgctga aaatgctgca acacgtcatc
    53761 cgtaccgcca accgcatgga caaagacgtg tccgtatgcg gcgagatggc gggcgatacc
    53821 gcgtttaccc gcgttttatt gggtatgggg ctgcgccgtt tttccatgaa tccgaacaac
    53881 atcctgcccg tcaaaaacat cattctgcac agcaatgccg gacagctcga aggcgatatt
    53941 gcgaaagtca tccgctgcga agacgaagag aagtcggaaa agctgatcaa acagatcaac
    54001 agcgtgtctg tcgcggaaga agccgaagtc aagggacgga aataaatatt gttgacaatg
    54061 cgagcgggtt gcagtcaaat aacgccctta aaaaatacag ctgctttggt caagaaatgc
    54121 cgtctgaaaa attttcagac ggcattttgt ttggcaggca tttctgccgc atcatcggaa
    54181 acggacgata cgcccttcat cgacgcggat gcggaccgta ccgtttccgg aaataccgtc
    54241 cgtatgttgt ccgacagtca ggtttaaggt cagctcgccg tgttcgggat ggacggcgga
    54301 aagccggagc gagtcgggca ggcggacgag ggacagcagg cggcattccg ttccatgatt
    54361 gtccaagcac acggcatttt gcggaatatg gcggtcatcg tcggtattgg gcagccccat
    54421 cagacgggcg acctgcacgc cggcaggcgt ttgaatcaag gtttcgggcg taccgcattg
    54481 aaggattttc ccctcgtgca tgacggcgat ttcgtccgcc gccgtgcagg cctcttcggg
    54541 cgaatgcgtt accaaaacgg cagggatgcc gcccttgcgg atgcgttcgg cggtcatacg
    54601 gcgcagccgg tcgcgcaaat gcgtgtccaa actggaaaac gattcatcca gcaacagcag
    54661 ggaagggcgg acaaccaaag cgcgcgccag tgccaaccgt tgcttctcgc ctccggaaag
    54721 tttttcaggc ttgcggtgcg cctcgttttc cagcccgact tcggcaagtg ccgacaaggc
    54781 gaggcgttcg gcttcggctt tcggcatttt ttgcattttc aaaccgaatg ccgtattttc
    54841 cagcgcactc atatggggaa acagcgcgta atcttgaaac atcagcgaga tacggcgttt
    54901 ttcgggcggc atacaggtaa tgttttcccc gttcagccga atttcgccgc cgtccggccg
    54961 gacgatgccc gcaatcatat tcagcagggt ggatttgccg cagcccgacc gccccagtac
    55021 ggcgagtatt ttgccgcgcc cgacagtcag gcagatgttg tcggcgaccg ttttgccgcc
    55081 gaagcatttg cagagtccgt tcagttcaag catggcgcat cctataaacg tatgccgtcc
    55141 agccactcgg acagcggatg ggcggcatca atcagaccgt aacggtaaaa cgcgtccagc
    55201 gtaaccagtt gcgcgtcgtg catcatgttt ttcgacaaca tggcatccaa taggccgcca
    55261 atgtccatct tttcaaaacc cgctacctcg ccatcctgat tttcaggcag gaaggtttcg
    55321 ggcaggacgg catcgaacac atacaggatt tcattgtgca cacctcggct gacggggcga
    55381 aggctgtgca gccgcgatac tgggcggatg agcggaaaca gcgttttatc caaaccggct
    55441 tcttcgctgc tttcgcggca cacggcttca gacggcattt cgccgccgga aacaccgccg
    55501 ccggcaatat tgtcgagctt gccgggatcg actgctttgt gcggactgcg cctgcctatc
    55561 caaaaatgcc atctgccgtt cgattcgacc aaaccgttga gatggacggc gcggctgagt
    55621 agtccgaacg gacggaaagc ggcgcgttcg agcgtgaaca aggggttgcc gccgccgtcg
    55681 gtcaggtcga aacactcgtt gcgccatccg tgaagcagcc ccgccttgtt ccatgtgcgg
    55741 gcgaggtgct gcaagcgtcc gcccatatcc ggccagccgt ccgcattcag aaaaatgccg
    55801 tctgaagact cggagcagcc tgcctcccag tcttttttga tgcgctcagc ccattccggc
    55861 gacagattgc ccaaaggaag acggttcaga tacagcgttt tccagcaact ttcggcaccg
    55921 taacttgctt ttgcccgctc gaacagggca tcaaggtctt gtttgctgac ggattcggta
    55981 aaacggacgg tcggcatagc cggctttctt gaacacaatg cggaagattg tagccaaacc
    56041 gcctgaattt gcaaccgcaa acagacggcg gcctgccgtt ccgatattgc ggcaggaagg
    56101 aaaatccggc aaaaaacgga ggcggcagga aacaggaaaa acaaaacccc ctcggcattg
    56161 aaccgagggg tttgatattt ggttgcggga gcaggattcg aacctacgac cttcgggtta
    56221 tgagcccgac gagctaccat gctgctccat cccgcgtcag aagatgaaac tatacggcag
    56281 attttttatg ttgtcaaaca ttatttccgt gaaaaatcat aaagttttcc gtccggcggc
    56341 ttatgcctga tttgaaatat cattttcttt acaaaagttc atgttcgtga tttaattttg
    56401 gttaacatcg aaacaggggt gctgcctgat gttcaggcgg ctgagaaata ccctttacac
    56461 ccgatcggga taatacctgc gtggggagtt ttcacggatt ctgtctttca gacggcattg
    56521 gttttcaaat gccgtctgaa aacgcaaaac gctcctgttt ctttattcta aacgagaaaa
    56581 caggagcatt ttttatgact acgccaaaaa aaaccgccaa aatttccggc aacgaagcgc
    56641 gcgaactttc cgacttgagc gaagacatcg gcatccgctt caaatatccg aactccgaac
    56701 gcgtgtatct gcaaggcagc cgcgacgaca tccgcgtacc tttgcgcgaa atccgtcagg
    56761 acgacaccta cacggcgcaa ggtacggaag ccaacccgcc tattccggtg tacgacacca
    56821 gcggcgcata cggcgatccg gcggcgcaca tcgacctgaa acaaggcctg ccgcacatcc
    56881 gcacggcgtg gctggacgaa cgcggcgata ccgaaatcct gcccaaactc tccagcgaat
    56941 acggcatcga atgcgcacac gatccgaaaa ccgcccatct gcgcttcaac caaatcaccc
    57001 gcccgcgccg tgccaaagcc ggccgcaatg taacccagct ccattacgcc cgccaaggca
    57061 ttatcacgcc cgaaatggag ttcgccgcca tacgcgaacg catgaagctg gacgagcttt
    57121 tcagacggcc tgaatacgcc aagctcttga aacagcacac agggcaaagt ttcggtgcga
    57181 acatcccgac ccgtcccgac caaatcaccc ccgaattcgt gcgccaagaa atcgccgccg
    57241 gacgcgcgat tatccccgcc aacatcaacc accccgaact cgaaccgatg attatcggcc
    57301 gcaacttccg cgtcaaaatc aacggcaact tgggcaattc cgccgtaacc tccagcctga
    57361 ccgaagaagt cgaaaaaatg gtgtggtcgc tgcgttgggg cgcggacacg attatggact
    57421 tatccacagg cgcgcacatc cacgaaacgc gcgaatggat tatccgcaac gcgcccgtcc
    57481 ccatcggcac agtgccgatt taccaaactt tggaaaaaac cggcggcatc gccgaagatt
    57541 tgacttggga tttgttccgc gacaccttaa tcgagcaggc ggaacaaggc gtggactatt
    57601 tcaccataca cgcgggcgtg ttgctgcgtt atgtgccgat gaccgccaac cgcctcaccg
    57661 gcatcgtatc gcgcggcggt tcgattatgg cgaaatggtg tttggcacat catcgggaaa
    57721 acttcctcta cacgcatttc gacgaaatct gcgaaatcat gaaggcgtat gacgtatcgt
    57781 tcagcttggg cgacggcctg cgccccggct gcattgccga tgccaacgac gaatcccaat
    57841 tcgccgaatt gcacaccttg ggcgaattga ccgataaagc gtggaaacac gacgtacaag
    57901 tcatgatcga aggccccggc catgtgccgc tgcaacgcgt caaagaaaat atgaccgaag
    57961 agttgcaaca ctgctttgaa gcaccgtttt acacactcgg cccgctcgtt accgacatcg
    58021 cccccggcta cgaccacatc acttcgggca taggcgcggc caatatcggc tggtacggca
    58081 cggcgatgct ttgttacgtt acccccaaag aacatttggg gctgcccgac aaagaagacg
    58141 tgcgcaccgg catcatcacc tacaaactcg ccgcccacgc cgccgacctc gccaaaggct
    58201 ggccgggcgc gcaattacgc gacaacgccc tgagcaaggc gcgtttcgaa ttccgctggc
    58261 gcgaccaatt ccgcttaagc ctcgaccccg aacgcgccga gagcttccac gacgaaaccc
    58321 tgcccgccga aggcgcgaaa atcgcccatt tttgctcaat gtgcggcccc aaattttgct
    58381 cgatgaaaat cacgcaggaa gtgcgcgact acgccgacaa gcaaaaagcc caacggcagg
    58441 gtatggagga aaaagcggtc gagttcgtca aaaaaggcgc gaagatttac agttgaaacg
    58501 tcaagcaaaa aatgccgtct gaagcccgga aaaaaggctt cagacggcat tttttcgctt
    58561 gtgaaaatga ttaaacaacc gatcattaaa gtaattttta aaatttttat attcacatag
    58621 atgggaatga tggatttagg gttctgattt tgttttgaga gaatgaagga atttgagatt
    58681 gtgggcgatt atcgggaaaa atag
//
LOCUS       DAWTQS010000005        49124 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.005, whole genome shotgun sequence.
ACCESSION   DAWTQS010000005 DAWTQS010000000
VERSION     DAWTQS010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 49124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49124
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.005"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            <1..574
                     /locus_tag="WNA25_000319"
     CDS             <1..574
                     /locus_tag="WNA25_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFA9823165.1"
                     /translation="DLSTAVDLSRALVEGGIPTLEITLRTPVGLEAIRLIAKEMPNAI
                     IGAGTVTNPEQLKAVEDAGAVFAISPGLHESLARAGRNSGIPLIPGVATPGEVQLALE
                     HGIDTLKLFPAEVVGGKAMLKALYGPYADVRFCPTGGISLATAPDYLALPNVLCVGGS
                     WLTPKEAVKNKDWDTITRLAKEAAALKPKA"
     gene            complement(763..948)
                     /locus_tag="WNA25_000320"
     CDS             complement(763..948)
                     /locus_tag="WNA25_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823166.1"
                     /translation="MGYFGGNGGYLKRKQPKNLWLDVGCLKKRNFAKVSERYFLTTVN
                     LLVSAAHHGILPIGRFL"
     gene            complement(959..1071)
                     /locus_tag="WNA25_000321"
                     /pseudo
     CDS             complement(959..1071)
                     /locus_tag="WNA25_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(1169..2209)
                     /locus_tag="WNA25_000322"
     CDS             complement(1169..2209)
                     /locus_tag="WNA25_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HFA9823167.1"
                     /translation="MKAMVYHGANDIRFEEKPRPQIIDPTDAVVKIVKTTICGTDLGI
                     WKGKNPEVADGRILGHEGIGIVEEVGEAVKNIKVGDKVIISCVSKCCTCDNCKIQLYS
                     HCRNGGWILGYMIDGTQAEYVRTPYADNSLVPPHDNVNEEIALLLSDALPTAHEIGVQ
                     YGDVKPGDTVFIAGAGPVGMSALLTAQLYSPAAIIVCDMDENRLKPAKELGATHTISP
                     ASGDVSKQVFAIVGEDGVDCAIEAVGIPATWNMCQDIVKPGGHIAVVGVHGQSVDFKL
                     EKLWIKNLAITTGLVNANTTEILMKAIFSSSVDYTKMLTHRFKFSELEKAYDVFKHAA
                     ENQAMKVVLEAD"
     gene            2372..3412
                     /gene="mutY"
                     /locus_tag="WNA25_000323"
     CDS             2372..3412
                     /gene="mutY"
                     /locus_tag="WNA25_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFA9823168.1"
                     /translation="MNTPIPFSERLIRWQKQHGRHHLPWQVKNPYCVWLSEIMLQQTQ
                     VAAVLDYYPRFLEKFPTVQTLAAAPQDEVLSLWAGLGYYGRARNLHKAAQQIVGQFGG
                     TFPSERKDLETLCGVGRSTAAAISAFAFNRRETILDGNVKRVLCRVFAQDGNPQDKKF
                     ENSLWTLAESLMPSENADMPTYTQGLMDLGATVCKRTKPLCRQCPMADICEAKKQNRT
                     AELPRKKTALEVQTLPLYWLIVRNRDGAILLEKRTAKGIWGGLYCVPCFESLNGLSDF
                     AAKLSLTMADMDEQTALTHRLTHRLLMITPFEGQMPSEHHSDGIWIKPGHLKDYGLPK
                     PLEIYLNGNRLE"
     gene            complement(3788..4156)
                     /locus_tag="WNA25_000324"
     CDS             complement(3788..4156)
                     /locus_tag="WNA25_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823169.1"
                     /translation="MYDKIHKSCRFFAGSFCFMFGAVPDNRVKFPLAHYFKIEILYFI
                     SLLLKRCSHFFIGFIIHHLIYGFWRQGLTAFAAVIDFWNRGNAFKESKAIRPDADGGA
                     IIHLLEFVAIGADSCDDYVE"
     gene            complement(4174..6030)
                     /locus_tag="WNA25_000325"
                     /pseudo
     CDS             complement(4174..6030)
                     /locus_tag="WNA25_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009119804.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
     gene            complement(6068..6301)
                     /locus_tag="WNA25_000326"
     CDS             complement(6068..6301)
                     /locus_tag="WNA25_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
                     /protein_id="HFA9823170.1"
                     /translation="MGAGGSEAAAPIIGKWLYGKGDGGSLNAEEKETVSAITRMLGTA
                     AGAAEGNSSADAVWGCFQTASDFASSFSYPINM"
     gene            complement(6728..7421)
                     /locus_tag="WNA25_000327"
                     /pseudo
     CDS             complement(6728..7421)
                     /locus_tag="WNA25_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
     gene            complement(7936..12208)
                     /locus_tag="WNA25_000328"
                     /pseudo
     CDS             complement(7936..12208)
                     /locus_tag="WNA25_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714280.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
     gene            12409..12696
                     /locus_tag="WNA25_000329"
                     /pseudo
     CDS             12409..12696
                     /locus_tag="WNA25_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009117427.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            13244..14581
                     /gene="ribB"
                     /locus_tag="WNA25_000330"
     CDS             13244..14581
                     /gene="ribB"
                     /locus_tag="WNA25_000330"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFA9823171.1"
                     /translation="MTDTAGLRRRNLRQWIAEHYGGLQNRFAEAVALNTGELSALLKN
                     KSFGEKKARKIEQAAKMPAFWLDTEHTGRPPGHTGKHTMSHISPIPEILADIKAGKMV
                     IITDAEDRENEGDLLMAAQFVTPEAVNFMIKHARGLVCLPMEGAMVEKLGLPMMTQKN
                     GAQYGTNFTVSIEAAHGITTGISAADRALTIQTAVSPTAKPADIVQPGHIFPLRAQKG
                     GVLVRAGHTEAGVDLAQMNGLIPAAVICEIINDDGTMARMPELMKFAEEHKLKIGTIA
                     DLIEYRSRTESLLEDMGDAPVQTPWGEFQQHVYVDKLSGETHLALVKGTPSADTETLV
                     RVHEPFSVMDFIQANPRHSWSLPKALERVQQAESGVVILLHHTEDGASLLDRTLPKGA
                     NQAYKWDSKSYGIGAQILAGLHVKKLRVLGQPSSFTGLTGFGLEVVGFEEAEK"
     gene            complement(14770..16371)
                     /locus_tag="WNA25_000331"
     CDS             complement(14770..16371)
                     /locus_tag="WNA25_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HFA9823172.1"
                     /translation="MTEQHFTEQIKSLIDSLKTICANYGLGNDGNEFKIISQAFLYKF
                     LNDKYDFEVKKIRKEKPDEPIEFVNMDIDGKTAVLKPEHSIKYLSERQNGADFAKLFD
                     DTLTDIAACNADLFSVKTEGGAKIVLFERISQYITDEGRRDDFCRALISKLAGFSFEA
                     IFAQKFDFFATIFEYLIKDYNSNSGGKYAEYYTPHAVARIMADILVPEEVRGQIRSVD
                     VYDPSAGSGTLLMNVAHVIGEDKCMIYTQDISQKSSNLLRLNLSLNNLVHSLNNVVQG
                     NTILSPAHKDASGCLKKFDFIVSNPPFKLDFSDFRDRLESDENHERFFAGIPKIKPTK
                     KEKMEIYQLFIQHILFSLKENGKAAIVLPTGFITAKSGIDKKIREYLVENKMLAGVVS
                     MPSNIFATTGTNVSILFIDKVNKDKVVLIDASGLGEKISINDNQKTVLSHEEEQKICH
                     TFTHKQAVEDFSVVVGYDEIKAKNHSLSAGQYFEVKIDYVDISADEFVQKIAGFSADL
                     DKLFAESAELEKEIKDRLAMLKFNS"
     gene            complement(16438..17277)
                     /locus_tag="WNA25_000332"
     CDS             complement(16438..17277)
                     /locus_tag="WNA25_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823173.1"
                     /translation="MNFVLAKITCKKDLVKILSDDHIFPDFSYENLNFITYNYDYNLD
                     DDTWFQIENLKNQDFCPKFLDNSNLFDSKMFSEIKKEEINIEKLKYLVSCTNDALFFQ
                     KITSSLLLKKKHLLTICGNGAKLCEPQDLLVIKDIPDAVYIIKDDKLIFRTLSSISNI
                     FKGIEDLYREATNTEVQQFLESDFIDLKEDFLSEKVSIPNRKRIALVQDRLNNMTLDQ
                     RQELLNYLAEYNNILKFNADGSRVEISTDVQLKHLLYGIDERYYTTALGKEKRLANSV
                     QPI"
     gene            complement(17288..17848)
                     /locus_tag="WNA25_000333"
     CDS             complement(17288..17848)
                     /locus_tag="WNA25_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823174.1"
                     /translation="MDKLFKILLNISSTSLLLSIYFIKSQYYILDLTWFYRVISNHWI
                     EPISKLSLLFYFLIPFIATATVLWLSKYLGKDEFKQGEVKELEYVNDNFLPSYLGYFF
                     VALSIPDNNLFLLFVMYGIIFLLVSCSKSFYFNPVFFLFGYRFYQAKTESGLLLVLIS
                     KQEFRTPQSVSSIAVYRINNFTFLEK"
     gene            complement(17841..19043)
                     /locus_tag="WNA25_000334"
     CDS             complement(17841..19043)
                     /locus_tag="WNA25_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFA9823175.1"
                     /translation="MNHFKKQQIQNIADFNPREQLAKGALAKSVPMAMLKEFQRQITG
                     YEIKAFNGGAKFRNGDTLLAKITPCLENGKTAFVDILDDGEVAFGSTEFIVLRAKNET
                     NPEFLYYFAISPDFRKRAIECMEGTSGRQRVNENALKTLELPIPEPQIQQSIAAVLSA
                     LDKKIALNKQINTRLEEMAKTLYDYWFVQFDFPDANGKPYKSSGGDMVFDETLKREIP
                     KGWGSIELQSCLAKIPNTTKILNKDIKDFGKYPVVDQSQDFICGFTNDEKSILNPQDA
                     HIIFGDHTRIVKLVNFQYARGADGTQVILSNNERMPNYLFYQIINQIDLSSYGYARHF
                     KFLKEFKIILPSKDISQKYNEIANTFFVKVRNNLKQNHHLTQLRDFLLPMLMNGQVSV
                     RCSGARDG"
     gene            complement(19093..19323)
                     /locus_tag="WNA25_000335"
                     /pseudo
     CDS             complement(19093..19323)
                     /locus_tag="WNA25_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004143539.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
     gene            complement(19372..22377)
                     /locus_tag="WNA25_000336"
     CDS             complement(19372..22377)
                     /locus_tag="WNA25_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFA9823176.1"
                     /translation="MSQTHNENSRVKIPAVLHLMRLGYDYLSLKNANWDRQTNIFPEI
                     FVDSLCRINPDLPPDDARRLLADIRLELDNEDLGQKFYERLTNQSGGKKLIDFQNFDN
                     NSFHVVTELPCINGDEAFRPDIALLVNGMPLVFIEVKKPNNKGGIGEERERMGKRAKN
                     PKFRRFINITQFMIFSNNMEYDDGATEPAQGAFYASSACGKPVFNYFREEHKLNLTEL
                     LDTLSDDLENNVLQDNNLPVIKHSPEFISNKSPDTPTNRILTSLLCRERLAFLLQHGL
                     TYVKASQGSVQKHIMRYPQLFATLAIEKHLSNGGKKGVIWHTQGSGKTAPAYYNTRYL
                     THYYAKQGIVPKFYFIVDRLDLLKQAQREFTARDLIVHTIDSREAFAADIKSAQTLHN
                     HAGKPEITVVNIQKFQDDPDVVARNDYDLAIQRVYFLDEVHRSYNPKGSFLANLNQSD
                     VNAVKIGLTGTPLIGVTAGNVNTRELFGDYIHKYYYNASIADGYTLRLIREEIGSRYK
                     AQLQEALAQLEIEKGSFDRKEIYAHPHFVHPMLDYILDDFAKFRKTNQDESLGAMVVC
                     DSAEQARQLFEHFQTASDHNFTAALILHDVGTKEERDQWVKDFKAGKTDILFVYNMLL
                     TGFDAPRLKKLYLGRLIKAHNLLQTLTRVNRTYKSYRYGYVVDFADIEREFDKTNRAY
                     WDELSNELGDEIGSYSQLFKTAEEIEQEIADIKNALFDFDTENAEEFCSQISQIEDKK
                     QLLALKKALQTAKELYNILRLQGSHEFLAHLDFDKLNLLYRETAARLDTLNLAEKLQQ
                     GDTAHLLNEALEDVYFQFVKIGEAELKLADDLKDIMRKVREGLAGNFDQDDLEYISLR
                     EELERIFKKKNLAEVGQEEMQANIATLQTVYTKIKEPNRQNDLLRHKYGGDAKYARIH
                     KRLMENAALYGDKLKVFNALNGVKTDADQRVLDMEQILDNQNYFEKQMQGIVLKRFRT
                     EQQFPVQPADIQTINRLLVREYLKESGRI"
     gene            complement(22497..23807)
                     /locus_tag="WNA25_000337"
     CDS             complement(22497..23807)
                     /locus_tag="WNA25_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFA9823177.1"
                     /translation="MTDLFVREPDAPLAERLRPHTLDDVIGQQHLIGEGKPLRVAVEG
                     GKPHSMLLWGPPGVGKTTLARILAQSFNAQFLPVSAVFSGVKDIRGAIDKAEIALQQG
                     RATILFVDEVHRFNKAQQDAFLPHVESGLLTFIGATTENPSFEVNPALLSRAQVYVLQ
                     SLSSDDLKKLIAKVLALPEYRDFTIEADVQELLVNTADGDARRLLNLLEQLLRAADTR
                     RLKILTTEFLADSLGAQIRRFDKGGESFYNQISALHKSVRGSHPNAALYWFCRMLDGG
                     TDPRYLARRIVRIAWEDIGLADPRAFQIANDAAATFERLGSPEGELALAQAVLYLAAA
                     AKSNAGYKAYNQMRRFVKENASDEVPVHLRNAPTKLMKELGYGREYRYAHDEPNAYAA
                     GESYMPDGLDEPDFYQPVPRGLEIKIGEKLAWLKSLDEEALKAK"
     gene            24078..25055
                     /locus_tag="WNA25_000338"
     CDS             24078..25055
                     /locus_tag="WNA25_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823178.1"
                     /translation="MELITTGEGADGDLLVNGSYGIRSFADLKLEYKPELSVRSNHDI
                     IFNNDFQSVDVQPYSGNIHLDAKGKIQFNLNVADKVTDPTTHQEKLVNDNALVRYVNN
                     ITPSDENDKGSISFHSKTGNLFNMNVNVHKGTHHFGIHNLRNTNVNLTAEDGDNVVAI
                     KGKLAEPMPAGKVESTSFLRGIETAFKSNTVLTGNNNKISITTEGDGLGLGVNGLQSN
                     SPYSGHTTGTFINNQPSNTTQPSLDGTSTIELKAVKGNNEVDLNVKNHASVKGIITSH
                     SAKATLEAEEDNIVRVKNPDTKTALINAWKEKYPNNSPEPYRVGGSIHD"
     gene            24985..25638
                     /locus_tag="WNA25_000339"
     CDS             24985..25638
                     /locus_tag="WNA25_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823179.1"
                     /translation="MPGKKNTPIIRQNHTGWGVQSTIDSDVVLSAANNIVEVGGSPMN
                     QEGITAHGNATITLKAKENNKITVENAAYSSDGISTLINRTGARPGTRDDGNKIILEA
                     GGDNIVTMKSGDADADYVNNSKVLTETPYYKSKRGSNGIFAYGDKSLVKLIGENNIVK
                     SEISEKSKALNGGFRHIGIYSWQNAKVELSAKSDNIVQGGIWGLYSNNSSISLKGKK"
     gene            25756..27360
                     /locus_tag="WNA25_000340"
     CDS             25756..27360
                     /locus_tag="WNA25_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFA9823180.1"
                     /translation="MNYKDDTRITGATVSDKGLVAIKPLNNTNIVADTIHYKGDVLAV
                     NKGKVELDFTPNIRLVGRLDNFSGLTDSKHKNLFENYVANLDSKSAGEINFNLAKDAL
                     WTMTGQSWLDKLEGQGTIDFNNDAKTSGRALHIGELAGANKFLMHLNKDGIHSDMLYV
                     KKGTSTPQEVVVKNLSEVLDSMNYGERLRFATVTNSKNEFVNGKKYIDDTHLMEDALT
                     VEYSAHNGDKNNKDDYNKSFNGSEMTAEKAGDDYVNKTYTDNRQNVYLVKQATGNPSR
                     NVKNINDMFDSTAHYAFTLDTYAKREGERAFSTLDKKEGDWIRLTHTRVIQSNAFRFH
                     NNDFEIGYDRFSLNEQEKKRKWGISLDYGHGRTSLWNTFGKDKIRKYELALYNTTQYI
                     DKEGDETGYIDNVLKIGKLRNRVIARNHMGQLWGKGKYSNTLFSISTEYGRRKFLDDD
                     KLWRITPQVQLQYSYLRGTGYRIDNGINVNLSHANSLIGRLGLDVVRKFDGGKKLFYI
                     KGNIFHEFLGSRSFKAFEGKSHYAQK"
     gene            27391..27862
                     /locus_tag="WNA25_000341"
                     /pseudo
     CDS             27391..27862
                     /locus_tag="WNA25_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691253.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(27888..28804)
                     /locus_tag="WNA25_000342"
                     /pseudo
     CDS             complement(27888..28804)
                     /locus_tag="WNA25_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255891.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            29059..30522
                     /locus_tag="WNA25_000343"
                     /pseudo
     CDS             29059..30522
                     /locus_tag="WNA25_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217121.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3360 family protein"
     gene            30773..31141
                     /locus_tag="WNA25_000344"
     CDS             30773..31141
                     /locus_tag="WNA25_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823181.1"
                     /translation="MNKTLSILPAAILLGGCAAGGNTFGSLDGGTGMGGSIVKMTVES
                     QCRAELDRRSEWRLTALAMSAEKQAEWENKICGCATEEAPNQLTGNDVMQMLNQSTRN
                     QALAALTVKTVSACFKRLYR"
     gene            31214..32623
                     /locus_tag="WNA25_000345"
     CDS             31214..32623
                     /locus_tag="WNA25_000345"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFA9823182.1"
                     /translation="MRYISTRGETAHKPFSEVLLMGLAPDGGLMLPEHYPQIGRETLD
                     KWRGLAYPELAFEIMCLFVTDIPEDDLRDILNRTYTEAAFGTKEITPVRTLSDGIKIQ
                     ALSNGPTLAFKDMAMQFLGNAFEYVLNKEGKKLNILGATSGDTGSAAEYALRGKKGVN
                     VFMLSPDGKMSAFQRAQMYSLQDGNIHNIAVKGMFDDCQDIVKAVQNDAAFKEKYHIG
                     TVNSINWGRIVAQVVYYFAGYFNATSSNDETVSFCVPSGNFGNVCAGHIAKQMGLPVR
                     CLIVATNENDVLDEFFKTGAYRPRNSAHTYVTSSPSMDISKASNFERFVFDLMDRDPQ
                     EINTLWAEVAAGKGFDLRFALDKVGGKYGFTSGKSTHADRLATIRQVYEQDKELIDPH
                     TANGVKVAREVREAGETVVCLETALAAKFDATIREAVGDVAIPRPAALEGLENLPQRV
                     QTVPNSADAVKGIIEQTLA"
     gene            32670..33464
                     /locus_tag="WNA25_000346"
     CDS             32670..33464
                     /locus_tag="WNA25_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823183.1"
                     /translation="MTLPMQETRFSILLDELAAKQEATIAPYLLADGTKVWVRKAGRH
                     NARWRYALLGMVARYLKLGVLKPVPSLGGEPAIATESKRLYELRSAGIAVPELLAVRK
                     NALMFGNLEGIPLDTQIRQEAEAGKADAWLAGLEAIARVHKKRQFLSQAFARNMMWDG
                     KNISFLDFEDDPSEVLTIAQCQARDWLCYIHSTALILKNGGLLEAAAEKWGGVLSDQP
                     AEIQKLIAGTVKPILPIRRLEHPRWGRDALRLAASISLISLADMPP"
     gene            33652..34428
                     /locus_tag="WNA25_000347"
     CDS             33652..34428
                     /locus_tag="WNA25_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HFA9823184.1"
                     /translation="MAAFNTQKVLSVHHWTDAYFTFTCIRDESLRFENGQFVMVGLMA
                     DGKPLMRAYSVASANWEEHLEFFSIKVQDGPLTSRLQHLKVGDEVLISKKPTGTLVAC
                     DLNPGKHLYLLSTGTGIAPFLSITKDPEIYEQFEKIILVHGVRYKKDLAYYDRFTKEL
                     PEHEYLGDLVKEKLIYYPIVSREEFEHRGRLTDLMVSGKLFEDIGLPKINPQDDRAML
                     CGSPAMLKDTCKVLDDFGLTVSPKTGVRGDYLIERAFVDQ"
     gene            complement(35087..37558)
                     /locus_tag="WNA25_000348"
     CDS             complement(35087..37558)
                     /locus_tag="WNA25_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFA9823185.1"
                     /translation="MKKTCFHCGLDVPENLHLTVRYEGEDRETCCVGCQAVAQSIIDS
                     GLGSYYKRRTADAKKTELPPQEILDQIRLYDLPEVQSDFVETHNGTHEAVLMLSGITC
                     AACVWLIEQQLLRTDGIVRIDLNYSTHRCRVVWDDGKIRLSDILLKIRQTGYTAAPYD
                     AQKIEAANQKERKQYIVRLAVAGLGMMQTMMFALPTYLYGGDIEPDFLQILHWGGFLM
                     VLPVVFYCAVPFYQGALRDLKNRRAGMDTPIAAAIIMTFIAGIYSLATNAGQGMYFES
                     IAMLLFFLLGGRFMEHIARRKAGDAAERLVKLIPAFCHRMPGYPAVQDVRESAVVKLQ
                     AGDIVMVKPGETIPVDGTVLEGNSAVNESMLTGESLPVAKMPSEKVTAGTLNTQSPLI
                     IRTDRTGGGTRLSHIVRLLDRALAQKPRTAELAEQYASSFIFGELLLAVPVFIGWTLY
                     ADAHTALWITVALLVITCPCALSLATPTALAASTGTLAREGILIGGKQAIETLSQTTD
                     IIFDKTGTLTQGNPAVRRIELLGSMTEAQVLAVAQSLEQQSEHPLARAILNCRISGGS
                     VPEIQVGQRLNRIGEGVGAQLTVNGETQVWALGRASYVAEISGKEPQTEGGGSAVYLG
                     SQSGFQAVFYLQDPLKDSAAEAVRQLAGKNLTLHILSGDREEAVAETARALGIAHYRA
                     QAMPEDKLEYVEALQKEGKKVLMIGDGINDAPVLAQADVSAAAAGGTDIARDGADIVL
                     LNEDLRTVAHLLDQARRTRHIIRQNLIWAGAYNIIAVPLAVLGYVQPWIAALGMSFSS
                     LAVLGNALRLHKRGKMPSEKILSEQ"
     gene            complement(37555..38733)
                     /gene="hflX"
                     /locus_tag="WNA25_000349"
     CDS             complement(37555..38733)
                     /gene="hflX"
                     /locus_tag="WNA25_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFA9823186.1"
                     /translation="MSGRTGRNSATQAQPERVMLVGVMLDKDDTGSNAARLNGFQTAL
                     AEAVELVKAAGGDSVRVETAKRDRPHTALFVGTGKAAELSEAVAADGIDLVVFNHELT
                     PTQERNLEKILQCRVLDRVGLILAIFARRARTQEGRLQVELAQLSHLAGRLIRGYGHL
                     QSQRGGIGMKGPGETKLETDRRLTAHRINALKKQLANLKKQRALRRKSRESGRIKTFA
                     LVGYTNVGKSSLFNRLTKSGIYAKDQLFATLDTTARRLYISPACSIILTDTVGFVSDL
                     PHKLISAFSATLEETAQADVLLHVVDAAAPNSGQQIEDVENVLQEIHAHDIPCIKVYN
                     KTDLLPSEEQNTGIWRDAAGKIAAVRISVAENTGIDALREAIAEYCAAAPNTDETEMP
                     "
     gene            complement(38739..40019)
                     /locus_tag="WNA25_000350"
     CDS             complement(38739..40019)
                     /locus_tag="WNA25_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HFA9823187.1"
                     /translation="MKSLFIWLLLLGSAAGVFYHTQNQSLPAGELVYPSAPQIRDGGD
                     ALHYLNRIRTQIGLHALAHAPVLENSARRHARYLTLNPEDGHGEHHPDNPHYTAQKLT
                     ERTRLAGYLYNGVHENISTEEEAAESSDSDIRTQQRQVDALMSAVYHRLSLLDRHTDE
                     AGAAFVRENGKTVLIFNQGNGSFERACAKGRRQPEAGRKYYRNACHNGAAVYADEAMP
                     VTELLYTAYPVGGGALPYFYGERPDPVPEYEITGNPASIDFSEAAGKIAMKSFKLYQG
                     KNEIRPVRVLTAGNDPNGRLTAHQFALFPLKPLEYGTLYTAVFDYVRNGRRAQAKWQF
                     RTRKPDYPYFEVNGGETLAVRKGEKYFIHWRGRWCLEACTRYTYRRQFGNSLSILRHE
                     AGGIVFSVSGMAGSRIRLTPEDSPERGVTLYLQD"
     gene            complement(40171..40896)
                     /locus_tag="WNA25_000351"
     CDS             complement(40171..40896)
                     /locus_tag="WNA25_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFA9823188.1"
                     /translation="METQNKPTVTDIDRPILVPPGGHKKVLLHSCCAPCSGEVMEAML
                     ASGIDYTIYFYNPNIHPHKEYMLRKEENMRFAEKFGIPFIDKDDDYENDRKEWFAKAK
                     GMEFEPERGIRCTMCFDMRFEKAAQYAHEHGFPVFTSSLGISRWKNMAQINDCGHRAA
                     APYDDVAYWDFNWRKGGGGARMIEISKRENFYQQEYCGCAYSLRDSNAHRKSQGRIPV
                     KLGVLYYGDESTQYEPAPVRVDK"
     gene            complement(40969..41646)
                     /gene="radC"
                     /locus_tag="WNA25_000352"
     CDS             complement(40969..41646)
                     /gene="radC"
                     /locus_tag="WNA25_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFA9823189.1"
                     /translation="MSIKQWPEGERPREKLLERGAAALSDAELLAILLRVGTRGMSAV
                     DLARYLLQEFGSLGRLMSAEVGKLSAYKGMGTASFTQFAVVREIGRRILEEELQEEIT
                     LSDPDTVADYLRFHLGQEKVEVSVALLLNRQNQLIAVRELSRGTVAENTIYIREIVKL
                     ALDEYADSLIIAHNHPGGSPEPSQEDIMFTRRLAQAMSLVDVSLLDHFIVTSQTVRSF
                     RQLGLMP"
     gene            complement(41769..43118)
                     /gene="gshA"
                     /locus_tag="WNA25_000353"
     CDS             complement(41769..43118)
                     /gene="gshA"
                     /locus_tag="WNA25_000353"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFA9823190.1"
                     /translation="MKLPVMSPEHSAQLQAFEAKILSNHAKIEAWFRTQWNAHRPPFY
                     GSVDIRNAGYKISSIDMNLFPGGFNNLNPNFIPLAAVAAQDAVQRACETEKSVLIIPE
                     NHTRNTFYLQNVYALGEIFRSAGCEVRLGSLNPEVTEPAEFETALGDKILLEPLLRTR
                     DRVHLADGFSPCVVLLNNDLSAGIPDILKGIGQTVLPPLHGGWTTRRKTNHFGAYNQV
                     AAEFAKLIDIDEWQINPYFEKIGGLDFQGREGEDALAEAVERVLAKIQAKYDESGIAD
                     KPFVIVKADAGTYGMGVMSVKSSDEVRGLNRKNRNKMAKVKEGLEVSEVIVQEGVYTY
                     ETLNGAVCEPVVYMMDRFVIGGFFRVHEGRGADENLNAGGMVFVPLSNSIPTGNGDNS
                     QEAPEACKRVFEQWDSLGMPRSEKDCDVDNEHNRLYVYGVMARLSLLAASIELEETA"
     gene            43370..44779
                     /gene="leuC"
                     /locus_tag="WNA25_000354"
     CDS             43370..44779
                     /gene="leuC"
                     /locus_tag="WNA25_000354"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFA9823191.1"
                     /translation="MTAQTLYDKLWNSHVVREEGDGTVLLYIDRHLVHEVTSPQAFEG
                     LKMAGRKLWRIDSVVSTADHNTPTGDWDKGIQDPISKLQVDTLDQNIKEFGALAYFPF
                     MDKGQGIVHVMGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHTMATQCI
                     TAKKSKSMLIAVDGKLKAGVTAKDVALYIIGQIGTAGGTGYAVEFGGEAIRSLSMEGR
                     MTLCNMAIEAGARSGMVAVDQTTIDYVKGKPFAPEGEAWDKAVEYWRTLVSDEGAVFD
                     KEYRFNAEDIEPQVTWGTSPEMVLNIGGKVPNPAEETDPVKRSGIERALEYMGLKAGT
                     PLNEIPVDIVFIGSCTNSRIEDLREAAAIAKGHKKAGNVQRVLIVPGSGLVKEQAEKE
                     GLDKIFIEAGFEWREPGCSMCLAMNADRLAPRQRCASTSNRNFEGRQGNGGRTHLVSP
                     AMAAAAAVTGHFTDIRTMA"
     gene            44874..45128
                     /locus_tag="WNA25_000355"
     CDS             44874..45128
                     /locus_tag="WNA25_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823192.1"
                     /translation="MNKLFVTALSALALSACAGTWQGAKQDTARNLDKTQAAAERAAE
                     QTGNAVEKGWDKTKEAVKKGGNAVGRGISHLGKKIENATE"
     gene            45190..45831
                     /gene="leuD"
                     /locus_tag="WNA25_000356"
     CDS             45190..45831
                     /gene="leuD"
                     /locus_tag="WNA25_000356"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFA9823193.1"
                     /translation="MKAFTKITAIVAPLDRSNVDTDAIIPKQFLKSIKRSGFGPNAFD
                     EWRYLDHGEPGMDNGKRPLNPDFSLNQPRYQGAQILLTRKNFGCGSSREHAPWALDDY
                     GFRAIIAPSFADIFFNNCYKNGLLPIVLTEEQVDRLFKEVEANEGYRLSIDLAEQTLT
                     TPGGETFTFDITEHRKHCLLNGLDEIGLTLQHADKIKAFEEKRRQSQPWLFNG"
     gene            45998..47269
                     /locus_tag="WNA25_000357"
     CDS             45998..47269
                     /locus_tag="WNA25_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFA9823194.1"
                     /translation="MQQIKFIDLFSGMSGIRKGFEQACRKQSVACECVFTSEIKPAAL
                     EVLKQNYPDEVPYGDITKIETGDIPDFDILLAGFPCQAFSFAGKRLGFEDTRGTLFFD
                     VARILKAKKPKGFILENVEGLVTHDRKDSTQKIGRTLTVILETLEALGYYVSWKVLNA
                     KDFGIPQNRKRIYLTGSLKSKPDLSFETSPSPKLKNILESGLPTESSPFIKKLLKKFP
                     PSELYGKSVKDKRGGKNNIHSWDIELKGAVTEEEKQLLNILLKERRKKKWASEIGIDW
                     MDGMPLTKAQISTFYKHPDLQNILDSLTDKGYLVLEHPKQKIGGQRIKDESLPKGYNI
                     VSGKKSFEINKILDPNDVAPTLVAMDMEHLFVVDNGGLRTLTGKEGLRLFGYPDDYSF
                     DIPKKDRCDLLGNTVAVPVIKAVSERLLHTL"
     gene            47285..48016
                     /locus_tag="WNA25_000358"
     CDS             47285..48016
                     /locus_tag="WNA25_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoBV family restriction endonuclease"
                     /protein_id="HFA9823195.1"
                     /translation="MIKLTAQQIFDKLLDEEKILSANGQIRFFLGDVDIIVKQKDVVG
                     NIIQEWLGGWLRKREIEFDVSTNTQMPPDFFLNKKDRSRELLEVKAFNRNACPGFDIA
                     DFKMYSDEIIHKPYMLDVDYLIFGYDMDDNGNVTIKDLWLKKVWQITRSMDGWAINLQ
                     VKKGVVHKIRPGVWYSINKKNMPMFECLEDFVSAIEETVYQNPATRHNASLWKKKFEE
                     AYKKHYNRSISIPRWHEIAHKYKKK"
     gene            48050..49120
                     /gene="leuB"
                     /locus_tag="WNA25_000359"
     CDS             48050..49120
                     /gene="leuB"
                     /locus_tag="WNA25_000359"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFA9823196.1"
                     /translation="MTKHIAILRGDGIGPEIVAETVRVLDKFIAQGLDADYEYAPLGG
                     EAYDEYGHPYPEFTQNLCRKADAVLLGAVGSPQYDNLDRPLRPERGLLAIRKDLNLFA
                     NLRPAVLYPELANASTLKPEIVAGLDILIVRELTGDIYFGEPRGIRVLENGEHEGYNT
                     MKYSESEIRRIAHVAFQSAQKRSKKVCSVGKANVLETTELWREIFEEIGKQYPDVELS
                     HMYVDNAAMQLVRAPKQFDVIATGNIFGDILSDEASMLTGSIGMLPSASLDENGKGLY
                     EPSHGSAPDIAGQNKANPLATILSLAMLLRYSLNDEARAQQVENAVQKVLQQGLRTGD
                     IYEEGTKLVSCSEMGDAVLAAL"
BASE COUNT        12170 a        12050 c        12042 g        12862 t
ORIGIN      
        1 cgacttaagc accgccgtcg atttgtcccg cgcccttgtc gaaggcggca tccccaccct
       61 cgaaattacc ttgcgcaccc ccgtcggact cgaggccatc cgcctgattg ccaaagaaat
      121 gcccaacgcc atcatcggcg caggtacggt aaccaatccc gaacagctca aagccgtcga
      181 agacgcaggc gcggttttcg ccatcagccc gggtttgcac gaatccctcg ccagagccgg
      241 ccgcaacagc ggcattcccc tgattcccgg cgttgccact ccgggcgaag tccaactggc
      301 tttggaacac ggcatcgaca cactcaaact cttccctgcc gaagtcgttg gcggcaaagc
      361 gatgctcaaa gccctctacg gaccttacgc cgacgtacgc ttctgcccga ccggcggcat
      421 cagcctcgcc actgcgcccg attacttggc attgcccaac gtcttatgcg tcggcggctc
      481 ttggctgaca ccgaaagaag ctgtaaaaaa caaagactgg gacaccatta cccgcctcgc
      541 caaagaagcg gcggcattga aacccaaagc ctgaaccgca tcgtaaaaat gccgtctgaa
      601 gccctttcca gtctcagacg gcattttgcc gcttggcgac tcagtcggca tacacagcag
      661 caccgcccct aaaatgccca tccatctcca gaacaatgcg aatgacgcgc gatatgcgga
      721 gacctatgac gaaaaaataa ttataatata tttatcaatt agtcataaaa atcgccctat
      781 cggtaaaatg ccgtgatgag cggctgaaac taacaagtta accgtcgtta aaaaataacg
      841 ctcagagacc tttgcaaaat tccttttctt caaacagccg acatccaacc acaggttttt
      901 cggctgtttt cgtttcaaat aaccgccgtt cccaccaaaa tatcccatta atccccctta
      961 aatatagtgg attaaattta aaccagtacg gcgttacctc gccttgccgt actattttac
     1021 tgtctgcggc ttcgttgcct tgtcctgatt taaatttaat ccactatacc tgattgatca
     1081 ggaacagccc gttttacacg gctgaaaaaa tgccgtctga aacacgttca gacggcatcc
     1141 gcttaaaaaa caactgattc aacggcgatt aatccgcttc caaaaccact ttcatcgctt
     1201 ggttttcggc ggcgtgtttg aacacgtcgt aggctttttc caattcgctg aatttgaaac
     1261 ggtgggtcaa cattttggtg taatccactg agctgctgaa aattgccttc atcagtattt
     1321 cggtggtgtt ggcattgacc aaaccggtag tgatggcaag atttttaatc cacagttttt
     1381 ccagtttgaa atcaacggat tgaccgtgta cgccgacgac ggcgatatga ccgccgggtt
     1441 tcacgatgtc ttggcacata ttccaagttg cagggatacc gaccgcttcg atggcacaat
     1501 ctacgccgtc ttcgccgacg atggcgaaaa cttgtttgga gacgtcgccg gaagcggggc
     1561 tgatggtatg ggtcgcgccc aactctttcg ccggtttcaa acggttttcg tccatatcgc
     1621 acacgataat ggcggcaggg ctatacagtt gggcggtcaa caaggcggac ataccgacag
     1681 ggccggcacc tgcgatgaat acggtatcgc cgggtttcac atcgccgtat tgcacgccga
     1741 tttcgtgggc ggtcggtaaa gcgtcgctca acagcagggc gatttcttcg ttgacgttgt
     1801 cgtgcggcgg cacgaggctg ttgtcggcat aaggcgtgcg gacgtattcg gcctgcgtgc
     1861 cgtcaatcat atagcccaaa atccagccgc cgttgcggca gtgcgaataa agttggattt
     1921 tgcagttgtc gcaagtgcag catttgctga cgcatgaaat aatgacttta tcgccgactt
     1981 tgatgttttt tacagcctcg ccgacttctt ctacaatacc gatgccttca tgaccgagga
     2041 tacgaccgtc ggcgacttcg gggtttttgc ctttccaaat acccaagtcg gtaccgcaaa
     2101 tcgtggtttt gacgattttc accaccgcat cggtcggatc gataatctgc gggcggggtt
     2161 tttcttcaaa acggatgtcg tttgcgccgt gataaaccat tgctttcatg ctgatactcc
     2221 ttgcttgttg ataaataatt tcaataccac aataaagttt ctttatatga gttatatgcc
     2281 cctacaaaaa ataagtcaat aagaattatt ttcacaattt tatacaataa catactgttt
     2341 taaatataaa taaaaccacc gattgatatt aatgaacaca cccatcccct tctccgaacg
     2401 gctcatccgc tggcaaaaac aacacggtcg ccaccacctc ccttggcagg tcaaaaaccc
     2461 ttattgcgtc tggctttccg aaatcatgct ccagcaaacg caggtcgccg ccgtgttgga
     2521 ctactatccg cgtttcttgg aaaaattccc gaccgttcag acgcttgccg ccgcgccgca
     2581 agacgaagtg ttgtcgttgt gggcgggctt gggctattac ggccgcgcgc gcaatctgca
     2641 caaagccgcg caacaaatcg tcggacaatt cggcggtacg tttccatcgg agcgcaaaga
     2701 cttggaaacg ctctgcggcg taggcagaag caccgccgcc gccatttctg cctttgcttt
     2761 caaccgacga gaaaccatct tggacggcaa cgtcaaacgc gttctctgcc gtgtttttgc
     2821 ccaagacggc aatccgcaag ataaaaaatt tgaaaactcg ctctggacac ttgccgaaag
     2881 cctgatgccg tctgaaaacg ccgatatgcc tacttacacg caaggtttga tggatttggg
     2941 cgcgaccgtg tgcaaacgga cgaaaccctt gtgccgtcaa tgccctatgg cggacatctg
     3001 cgaagcaaaa aagcaaaacc gcaccgccga gctgccgcgt aaaaaaactg ccctcgaagt
     3061 gcaaaccctg ccgctttact ggctgattgt ccgcaaccgg gacggcgcaa tcttgctgga
     3121 aaaacgcacc gccaaaggca tttggggcgg actgtattgc gtgccgtgtt ttgaaagttt
     3181 gaacgggctt tccgactttg ccgccaaact ctccctgacg atggcggata tggacgaaca
     3241 aaccgccctg acccaccgcc tgacgcaccg gctgctgatg attacgccgt ttgaagggca
     3301 aatgccgtcc gaacaccatt cagacggcat ttggataaag ccgggccatt tgaaagatta
     3361 cggcctgccc aagcctttgg aaatttattt aaacggtaat aggttagaat aaacaaaata
     3421 aacccgttga actgttgtta gcaggtatcg cagcaagaac aaccgatgaa tttgggtcgt
     3481 attttaggca gcgggataat gttcaaatgg gacatttgga acggaaaaag tcggcaattt
     3541 aaaaaggatt ttagaaacaa agagggtcaa aaacatgaac acaaacttaa atgacaaaga
     3601 caaagccatg gataccgcaa tcaggtttca gaaaaggatg aggattccga aatttttctt
     3661 tttaattctc ggaatcacag tggttttggc atttatccaa gacgtgataa cgggttctaa
     3721 ttttctgcaa ataacaatta tgtaaataat atttgtgata tatttcagtt tcggaggtat
     3781 cataatttta ttctacataa tcatcgcagg aatctgcccc tattgccacg aattccaaaa
     3841 gatgaattat ggcacctccg tcggcatcgg gtcggatagc tttactttct ttaaaggcat
     3901 ttcccctgtt ccaaaaatcg attactgcag caaatgcggt aagcccctgt ctccagaagc
     3961 cgtaaataag gtgatgaatg ataaaaccga tgaagaaatg tgaacagcgt tttaacaaca
     4021 gtgaaataaa atataaaatt tcaattttaa aataatgagc taatggaaat ttgacccggt
     4081 tgtccggtac ggccccaaac ataaaacaaa agctgcctgc aaaaaaccgg cagcttttgt
     4141 gtatcttgtc atacattatt cacttcaacc gcatcaaaac gaataaccca cctgaaaccc
     4201 cgttacccat ttcttcgtct gaaaatattc gggctttttc aatgcacggc cggtaaatat
     4261 atcgtaatgc aggttgccgc caagctttat ctgcccgcgt atcccaattg ctgtgccggc
     4321 tagagtttgg cccgataacc atttggcgga ttgtcctgaa acatgtccta catcagcccc
     4381 aagataaagc tgatggcctg gtttaaattg ccagctcaaa tcgttgcgcc aataccatcc
     4441 ccgctcggca ggcaaactca tttcaccgtc gaagccacgt acggtgtggt gtccgccgat
     4501 agccagtttg tcttgcgatg ttagcggggt tttgttccat tgtgcatgaa cggatgtgtc
     4561 ataggcaaat agctgtttac cgatttgaaa aggagtattt acatcagccg atgccgtcca
     4621 aattttcata cgtgacgtgc cttcgccaaa ggcttcttca ggcgcgcgca gagcatcttt
     4681 catgccggtg ccgtgtttat atttcaactt aaaatctgcc gtactgcgac cgatatatcc
     4741 tttgtgggaa agttctgcca accaacctgt ggttttacgc cgttgtacag tcagttcggc
     4801 atcatcaatg taactttttg tttccctcgt ccacagtttt acactgagat aggttttgcg
     4861 tttggcatca cgatacaaca ggcggttgaa gccgaaatca gtgttgtaac tttttccatt
     4921 atagtcatag acttccgata atccggaaac cgcctgatgg taacggtagc cattgtgatt
     4981 gaatgcccat gtccatttac cgaaaggggc tgaataatgt acggcgtaat tgtttgatcc
     5041 gccttctttg cgatggccgt caaaattttc ctcatcgggc gtaccgccaa ttgaacgtcc
     5101 ataatttaca tagaacatat cactcagtcc aaaaggattg tcggcagaga aagtgatatt
     5161 tccttggtat tttcctgtcg cctcactacc cgaattatcc atccccacac tcacacagta
     5221 gggcagcaga cgttaccgcc attgcaccac gacatcactt tggtttggtt ctctctctac
     5281 gggaacgatt tggagatcgg cttccgcagt cgggagacat ttgagatttt ccagtccttg
     5341 ttccaaatca cgcagattca acagatcgtt cgagcgggtg ggaaatttgt tttggaatgc
     5401 tgcaatacgt cctgcatggg tttgatcatc gttggaccga tcgattcgta tggagcgcag
     5461 atagcccggc atcagggtta attgaagctt gccactattc aaatcctgtg gcgcagccaa
     5521 gatacgggtc gtggtatatc ccctgccgat caaagcattt tgtgctaagg acatgatttg
     5581 attaatgtcg cccgcatgca gacacttgcc ggaaacaaaa tgtgtttggc acaaggcatg
     5641 gttgagcgca aaccggaatt tagccgcttc ttcacccacc aattccacct cagaaatagg
     5701 aaagcagggg cttgcctcat ccgaatttgc accgcctacc tgtgttgcca tcttctctat
     5761 gcccgtatcg gtaccatcca aacgaacatc ttgttccggc tgcatctgtt cacgcaattg
     5821 cttctcacgt tgacgttgga gaatatcctg ttgttgtgaa acagcttcag gtgtgggggc
     5881 ggctgatgtt agggtgggga ggcagccatc aatgatagga ctaacagtga taagggatag
     5941 ggatgaaata catttttgga ggaaaagaaa ggtttcatag gattggtcta agtaatgtaa
     6001 gcgggcaatt atagcaatgc acaaaaacaa acaaaaagat aaaaaaactc cataaatgaa
     6061 aatttaatca catgtttata ggatatgaaa aagaggaagc gaaatctgaa gccgtctgaa
     6121 aacaacccca cacagcatcg gcggacgagt ttccctcagc tgctccggca gccgtaccca
     6181 gcatccttgt aatcgccgaa acggtctctt tttcctccgc attcaggctg ccgccgtctc
     6241 ctttgccgta cagccatttg ccgatgattg gggcggccgc ttccgacccg cccgcaccca
     6301 atgctcctgc cggggcattg ttgcctcatg ctgcggcaac cgctgctcct aataccgcgt
     6361 gggcaagaac gtgagcggtt tcttgactgg cggttagttt gccattcgcg ttttgaccgg
     6421 ctaaatcttt aaagtgctgt ccaatcgcat acgatacgtc tggcgatgcg gtagccgcag
     6481 cgatgcccgc tccgctttgg gtcggctcag ctaaacctga ggctaacatg ttgagaatga
     6541 ctttgccttg ttgccaatta tctgcttttg ctgccgcatc ttgagcttca tgggctttgc
     6601 gtttggcagt ttccatatcg ccattggcta atgcctcggc tgctgctgtt tcggctgctt
     6661 ctttgtctgc tttgagtttg tctaaatgtt ggttaatctc ggtattggtt tgttgaacat
     6721 ttttactaaa atcttggctg acggttcttt gtaaatccag ttcactttgc actctatctt
     6781 tgtcaaatat gtttttcagg ctgcccgaat gtcgttcggc ggtgtctgtg gttacgtttg
     6841 tatcaatatc ggctttggtt tgtgccgcta ttttgcctgt cagcctgatt tgtgcggctt
     6901 cgtctgtgat ttgaatgttt ttgggtattg atgccgcttt ttgtgatgct gctttgactg
     6961 tcgctgtcgc tgccgtaccc tactgatgaa cttgcgccgt ttttatcggc tacgcttgtc
     7021 aggtgtttgt cttgaggttt attttttgcg ccctgtccca gtgttttgcc gcttacggca
     7081 acgctggcac ccaatccaaa actttttcct tcgtattggc tgtaattttg aatatcactg
     7141 ccggcgagtg tgcctgtgct gaatcggttt ttacccttgt cttttgcgct ttggctgctg
     7201 gtgatgatgc cgcctttgag gcctgtatgg tttccgacct tgatttgata gccgtcttct
     7261 ccggcataaa taccgctttg ctcggttacc gaagcatggt cggctcggat tttgctttgg
     7321 ctgtaatcgc cactggcact gaagccataa cctacggtaa cttgtgcacc ggcgttttgt
     7381 tgtttgcttt gataagtttc tctatcttgt acactttgaa tgcttaggtt tttggcattg
     7441 acttgtacgc ctttgccgcg tacttgcgcg cctttgatga tggtatcgcc accactttgg
     7501 ataagggttt ggctgccttt gtcgccaata tggctatggc ggtgggttac gctatcgcca
     7561 ttgccgtagc ctttgccgac attgccgcct gcggcaacgc ctaatgacca gcctccttgt
     7621 ccgaatgata cggcagcacc tgcgttccag cctgctgatt tgttttggct gcgttcggta
     7681 ttgctttgct cggctgattg cagatggatt tgcttatcgg caatcaggcc ggttcctgct
     7741 ctgcctgaaa cacctgagcc tgtgatgttg atattggatt gttcgccgca ccttcggcaa
     7801 taagggtagt tttgccgcct gcttgaattt gactcgcttg ggcttgattg gcttgaactt
     7861 gggtggtttg tcggttttgc tgttcgccgt aggttatgga gatgctgact tgtttggcat
     7921 tggttgtacc attggctaag ttttgtgcgc ctttgcctgt ttgataggcc tgccaacctg
     7981 cattggcagc cgccatggca ttaacgcggt cgtttttgct ttgtccgact tgtttgctgc
     8041 tttgtgtgct acggcaatcg cttgttgtgc caaatcggta acgggcgaac tgaatgccac
     8101 cgttaagcct ttttgttcgt aggtttgggt ggttttgctg tttaattggt tttgtgctgc
     8161 gccaatatcc atactttgcg tgctgataag gttgttgccc tcagggctgg aaacgttgct
     8221 gccggtttgt tcgtagtgtt tgcttgcaac aatggtggta tcgcctttca ggctgcctac
     8281 ggtactgcct gtatgttcgt tgctttggga ttggttttct tgtgtgtttg tcttgctgcc
     8341 aatagtgaag ccgatacctg cactcatcaa tcctgatttt tgggtttgat gataggtttc
     8401 gctttggctt tgagtttggg ttgtaccaat gcgaacatga ttgcctgctt gaatccgggt
     8461 gccattatcg gaaataacat tactgccaag gatgttggca tcgtttcctg cctgcaatac
     8521 aacttgcttg ccttcaaagg tgctgctttg agcagtttcg tgatgacttt gggctttatc
     8581 ggtaatgact aatttattac cgccgccgct tctgcctgta tgtttggacg catcatcaac
     8641 ttggccggca tggatgcctg agctgatagt aatgtcattt ttagcataca cggcaagtgt
     8701 gccttttgcg ctgccgactt cggcagcttt ggcattgaga ttattccctg acaatagggt
     8761 aacatcgcct tttgtttgaa tgctgctgcc gacttcgttc gttgaacctc ggatggtatg
     8821 gttatcggca tcaaaatgga tttcttgata tttgccggtt tgtaccgtat ccaggttaat
     8881 gtcgcgtcct gcctgcagcc gggtttgccc ttgatctgat tgattgctga tttgaccggc
     8941 aatgatgttg atgtctttgc ctgcctgcgc tgctaaaaca cctttttctt tgcctgtgat
     9001 ataaatacct gccattcggt ctaggtaggt gctgctacct tgtgcatttt gactgctctt
     9061 ggccgtgctt tggttgttga tgttgttacc cgcattgagc aataatgtct gttcggcaga
     9121 aagaatgccg ccaatattat tgatgtcttg tgtggccgta accgctgatt tttgcgcatg
     9181 aatacgccca ccgatattgt ctagcgtatc ggtattgata ataagcgcat tgcgccctgc
     9241 aatcgtgcct gagtttttca ggctgcctga aacattgatt tgtgtattgc tgcctgacaa
     9301 caatgcacct ttaccgtcta tgccgccatt tttaacgcgt acataaacct gtggcatcaa
     9361 tacggtttgt gtgccgccat caggaagttt aacttctttt tgtaccaacc aaacaatatc
     9421 gctggtcagt tgcgctgctt gctcggcact taatgcaatg ccaacgctga gattcatcga
     9481 acgtgccgca gtcgcgccat tatccattaa ggctttaaat tgttcttcgt cgttttgata
     9541 accgtctaaa cgacgatgcc ctgtcagctc tgcgatttgt tcattgatta aacgttgctc
     9601 gtaataacca tcacccaaac gtttatgtaa attgtttggg tctagtttga ggctgcccag
     9661 catatagtca ctacccaacc attgacggta gttggcaaag cgtggatcgg tttcaacaag
     9721 atagccttta ttggcaggat tgataatgta taagctgctg ccgggtaatg gggtaaaaga
     9781 attgggcgta tagggtagcg aaataccgtt gcttttcgca gtacggatat tatcccggtt
     9841 actttgtggc aactcagtgc cttggctggg cgcatgacgg cttaatgctt tgctatgcga
     9901 ttcataggca aatgaaccca gtgaaatgtc gcgtgtgatt tcctccggca aagtataatt
     9961 ttgttcacga tgccctgttt catcatgtcc tttacgacgc gcacgccagt agttgtgcaa
    10021 cttaccattt tcgctgaaga ctttcttctc gccaaaggtt tgctcgttat gcaaaccgtc
    10081 tttttctgtt tgcacaagca aattgccgcc ggcgatgatt cggctgtcgc tgttgaagac
    10141 tgctttgcta tcaataatca aatcgctacc tgcaatgatt ttagcaggcg cagttccggt
    10201 tacttgagtt tcttgcgtta ctttttcata atcgtattta tgccaatttt catgcgccac
    10261 tccatcaggg gtgcgtaagt ggtctgattc attgttgtag acaaaccagc ctaattcatg
    10321 ttgcgtgcct tctcgcaata attcgtgtcg tccaaatgct tcgtaatcaa caatacgctc
    10381 gcgccctgtt tctaccaact gcgttttcaa atgctcattg gtattgtgca gcttttctac
    10441 acctaaacgc attttgcctg cagcttcaat gattgcgccg gcattgtgta tcctttgggc
    10501 tttgcctgtg gcttggtcat tggtatctaa tgcgccgcca accgccatat cgttaccgct
    10561 gtaaatcaga ctgttttcac ggttatttaa ttgttcaatg cctaaattca ggttttcgcg
    10621 tgccgcaatg gcggcacctg taccgttttc atcttgattg tctaagcggg tagccgcaat
    10681 agcgatattg tcgccataaa tccgacctgt accgatatta ttcatttgcc cggcttggat
    10741 tttggtttgt tgtccgtcaa tcaagcctct attggttaaa ttgtgctgcg tgccaatgtc
    10801 tgtcgtaccg ccggattgaa tgttgccttg tactgcatta tcaaggttat ttgctttaat
    10861 ccgaatgcgt tttcctgctt gcaaggtatg tgaatttttc aggctgcctc gtgtactgag
    10921 cgacaattca ttgcccgcca cgatcttgcg ttctacataa aaatcatctt gtaacgcaat
    10981 atccagttta ttatcagcgg caagtgtgcc gttgttggat aacgattttg cctgaatggc
    11041 aacatcacgg cctgattgta tcgtgccatt cgtattatca atgacaacgg tagattgctg
    11101 accatcgtga ataatcagtt gttgattggt cgctatttcg ccattttgat tgttcaggct
    11161 gcctgaaacg gctaaatcct ctgttcctgc cgacaataat ttgccagtgt tttgaatcga
    11221 ttgactgtga atttgagtgc cttgttgcga taccgccgta ccgctgtttt caaaggcttg
    11281 actgcggata ttgactttgt gttccgctgt attattcata tcttgcacat tggcggcagc
    11341 catcgtgcca ctattgacca aacggccatt tgcatcaatc gccacattac cggaagaagc
    11401 aaacaactgc ccttgattac gaatgcctgc ttgttcggcc gtactgatca aggtgatttt
    11461 gttggcatac aagaatacgt gtgaaatcgg tatcacgggc atccaaaccg tgtccggcga
    11521 ttacaacatt gccttgcctt atcttaaagc cgctaaagtc tcctgcttga tattgcggtt
    11581 ggcctgtcgt caaagtggca cgggaagcat tgataaaacc accaccattg actgcaatcc
    11641 ctgccggatt ggcaataacg acttctgcac gtcgtccacc cacttcaata tagccattca
    11701 gttgtgaagg atggctgctg ttgatttggt ttacaaccac acgtgcttcg ccccttgtca
    11761 accaaggatt gccttgaatc caaccgccta gctgtgtttg ggtgttgctg cgactgttgt
    11821 ttaaaatcgc cccgcgatta cccacatcaa actgggcata ttgattaaca gaaacccctg
    11881 ccgaagtagg ggtttgaata ttgacttgcg gtatgccgtt acctgtttgc agaatcgtgg
    11941 cttgttgggt tttaggagca gctttatcag taataatgcc gtcagcaaaa gcaatattga
    12001 ccgtacccaa agccaaacat aaagaaaagc ctaatgcaga aaaacaaaag gctttggaat
    12061 gagtaggaat gaaagaaacg gatttcacat aaacgctgcc cgaaccacta tcggcacagc
    12121 ttttaccttc gcgcttggtg gtttcggcaa cagctaccac agcaccgcgt ttgcggttga
    12181 aaatcacacg atagagggtt ttgttcatga ttttagttat ttgattttta tagagttatt
    12241 acaaacattg gacagtctgg gcattctagg acaaagattt tggtaagtca attaccgcca
    12301 tttttttatt aaattagggg acgtaccgga taacggctaa aaatcccatt taggttagaa
    12361 taagccaatg agaaaagccg tctaagccgg cataaacaaa ataaactcat tgggctattt
    12421 gtcgcaggcg taactgcaag aacagcggca gagttggtag gcgttaataa aaataccgca
    12481 gcctatgatt ttcatcgttt acgatgactg atttatcaaa acggtccgca tttagaaatg
    12541 tttgatggcg aagtagaagc agatgaaagt tattttggcg gacaacgcaa aggtaaacgc
    12601 ggtcgcggtg ctgccggaaa agtcgcagta ttcaggcttc tcaaacgaaa tggcaaggtt
    12661 tcctttgccg tccgttatgt acgtttcgta acattcgaag acttcctgaa ccctgcccgc
    12721 cgtttcggtt tctgtacccc ctgttagata aggggggaag attcgaagcg gttgccggct
    12781 tcctgccgtc cgccggcgcg tccgtcatca cgccggcaac cgcctttttc atcccttgct
    12841 tatcttccat ttgcgaatcc tcaaaaacgg gcaaaaaaaa gccctgttac ttgtagaaag
    12901 taaaaggcga tccgtccggt ttttatcggg aaggcggcat tcatcatagg attgccggta
    12961 ttgcgcctgc tcgtaccgcc tatgccgtac ccggcaatca aggcttttta tcagaatttt
    13021 tttcggaata accctccgga aaccccataa tcagaagcgt gcgggaagaa acagccgccc
    13081 cgccggcggg gttgcggcaa tgccgtctga agccacgaat ccggcttcaa acggcatctg
    13141 tttaccaaaa ggcaaataat ccgatttggc gaaaaacaga tttgcttttt ggtaaatacg
    13201 cgcttacaat ccgctacatc cgatttctac aaaggataaa acgatgaccg acacagccgg
    13261 cttgcgccgc cgcaacctgc ggcaatggat agcggaacac tacggcggac tgcaaaaccg
    13321 ttttgccgaa gccgttgccc tcaacacagg cgaactctcc gcccttttga aaaacaaatc
    13381 cttcggcgag aaaaaagccc gcaaaatcga acaggcggca aaaatgcccg ccttctggct
    13441 cgataccgaa cacaccggcc gaccgcccgg acacacagga aaacacacca tgtcccatat
    13501 ttcccccatc cccgaaatcc tagccgacat caaagccggt aaaatggtca tcatcaccga
    13561 tgccgaagac cgcgaaaacg aaggcgacct gctgatggcg gcgcaattcg ttacccccga
    13621 agccgtcaac tttatgatca aacacgcgcg cggcttagtc tgcctgccta tggaaggtgc
    13681 aatggtcgaa aaactcggtc tgccgatgat gacccaaaaa aacggcgcgc aatacggtac
    13741 caactttacc gtttccatcg aagccgcgca cggcattacc accggcattt ccgccgccga
    13801 ccgcgccctg actattcaaa ccgccgtttc cccgaccgcc aaacccgcag acatcgtcca
    13861 acccggtcat atcttcccgc ttcgcgccca aaaaggcggc gtactcgtcc gtgccggaca
    13921 caccgaagcc ggcgtcgact tggcgcaaat gaacgggctg attcccgccg ccgtcatctg
    13981 cgaaatcatc aacgacgacg gcacgatggc gcgtatgccc gaactgatga aattcgccga
    14041 agaacacaag ctcaaaatcg gcacgattgc cgacctcatc gaataccgca gccgcaccga
    14101 aagcctgctc gaagatatgg gcgacgcgcc cgtacaaact ccgtggggcg agttccaaca
    14161 acacgtttac gtcgacaaac tctccggcga aacccacctc gccctcgtca aaggcacacc
    14221 ctccgccgac accgaaaccc tcgtccgcgt ccacgaaccc ttcagcgtga tggacttcat
    14281 ccaagccaat ccgcgccatt cctggtcgct gcccaaagcc cttgagcgcg tccaacaggc
    14341 cgaaagcggc gtcgtcatcc tcctgcacca caccgaagac ggcgcatccc tgctcgaccg
    14401 caccctaccc aaaggcgcaa accaagccta caaatgggac agcaaaagct acggcatcgg
    14461 cgcgcaaatc ctcgccggcc tccacgtcaa aaaactgcgc gtcctcggcc aaccgtcatc
    14521 tttcaccggc ctcaccggct tcggcctcga agtcgtcggc tttgaagaag cggaaaaata
    14581 atatagtaaa ttcaaatgct ttatatttgc ttcatttatt gcattatttc cgtacaaacg
    14641 aaaacccggt ctgttgggtt ggattttgct tttttcggat ttcgataagt attttccaaa
    14701 ccaaacaaaa tgccgtctga aaggctttca gacggcattt taagtttgac cggtttcatt
    14761 catcggtatt tatgaattga atttcaacat cgccaatcta tccttaatct ccttttccaa
    14821 ttcggcagat tcggcgaaaa gtttatccaa atccgctgaa aatcccgcta ttttttgcac
    14881 aaattcgtcg gcggatatat ccacataatc aatttttacc tcaaaatact gccccgccga
    14941 caagctgtga ttcttcgctt tgatttcatc atagccgacc accacgctga aatcttctac
    15001 tgcctgtttg tgcgtgaagg tatggcagat tttttgttct tcttcgtggg aaagtacagt
    15061 tttttggttg tcattaatac taattttttc acctaaaccg gaagcatcga ttaatacaac
    15121 tttatcctta ttgactttat caataaacaa gatagaaaca ttggttcccg tcgtagcgaa
    15181 aatattggaa ggcatagaca ccacgccggc gagcattttg ttttccacca gatattcgcg
    15241 aattttttta tcgatgcccg attttgccgt gataaagccg gtcggtaata caatcgctgc
    15301 cttgccgttt tctttcaggc tgaacagaat atgctggata aaaagctgat agatttccat
    15361 cttctctttc tttgttggct tgattttcgg gataccagca aaaaagcgtt cgtggttttc
    15421 atcgctttcc agccgatcgc ggaaatcgct gaaatccaat ttaaacggcg gattggaaac
    15481 aatgaaatca aattttttca gacagcctga agcgtctttg tgggcgggcg acaaaatcgt
    15541 attgccctga accacattat tcagcgaatg caccagattg ttcaaactca gattcagccg
    15601 caacaaattg gacgatttct gcgaaatatc ctgagtataa atcatgcatt tatcttcgcc
    15661 gatgacatgg gcgacattca tcaacagcgt tcccgaaccg gccgacgggt cgtaaacgtc
    15721 cacgctgcgg atttgcccgc gcacctcctc cggcaccaaa atatccgcca taatccgcgc
    15781 aaccgcgtgc ggcgtgtagt attcggcata tttgccgccg gaattactgt tgtagtcttt
    15841 aatcaggtat tcaaaaatcg tggcgaaaaa gtcgaatttt tgcgcaaaaa tcgcctcgaa
    15901 gctgaaaccc gccagcttgg aaatcagcgc gcggcaaaaa tcgtcgcgtc ggccttcgtc
    15961 ggtaatgtac tgactgatac gctcgaacaa cacgattttc gccccgcctt cggttttcac
    16021 cgaaaacaaa tccgcattac aggcggcaat atcggtgagc gtatcgtcaa agagtttggc
    16081 aaaatccgcg ccgttttgcc gctctgataa atacttgatg gaatgttcgg gctttaaaac
    16141 cgctgttttt ccatcaatat ccatattgac gaactcgatc ggctcgtcgg gcttttcttt
    16201 gcgaattttt ttaacttcaa aatcgtattt gtcgtttagg aatttataca aaaacgcctg
    16261 actgatgatt ttaaattcgt tgccgtcatt gcccaaacca tagttggcgc agatggtttt
    16321 taagctgtcg attagggatt tgatttgttc ggtaaagtgt tgttcggtca tggaatgtct
    16381 cggtttgttg ctagcgtcgc ttaaattttg tttagcatta gttggtttgc tggcagatta
    16441 aataggttga acactatttg ccaacctttt ctcttttcct aatgctgttg tgtaataacg
    16501 ttcatcaata ccatataaca aatgtttcag ctgtacatca gtagaaattt ctacacgaga
    16561 gccatctgcg ttaaatttca agatattatt atattcagca aggtaattta atagttcttg
    16621 tctttgatct agcgtcatat tattcaatct atcttgaact aaagctattc ttttcctatt
    16681 gggaatagat actttttcac ttaaaaaatc ttcttttaaa tcaataaaat cgctttctaa
    16741 gaattgctgt acttcagtat ttgtagcttc tcgatacaaa tcttcaatcc ctttaaatat
    16801 atttgaaata gaagaaaggg ttctaaatat taatttatca tcctttatta tataaacagc
    16861 atcaggaata tctttaataa ccaataagtc ttgtggttcg catagtttcg caccatttcc
    16921 gcatatagtt agcaagtgct tctttttgag taataatgaa cttgtgattt tctggaagaa
    16981 tagtgcatca ttagtacaag atactaaata ctttagtttc tctatattta tttcttcctt
    17041 ttttatttca ctaaacattt ttgaatcaaa taaatttgag ttatccaaaa acttaggaca
    17101 aaaatcttga ttctttaaat tttcaatttg aaaccaagta tcatcatcaa gattgtaatc
    17161 atagttatat gttataaaat ttaaattctc gtaagaaaag tcagggaaaa tatgatcatc
    17221 agataatatt tttactaaat ctttcttgca agtaatttta gctaaaacaa aattcattat
    17281 tgaactccta tttttctaag aaagtaaaat tattaattcg atatacagca atcgaactta
    17341 cactttgtgg cgttctaaat tcctgtttac taatcagcac taataataac cctgattcag
    17401 tttttgcttg ataaaatcga tagccaaata agaaaaaaac agggttaaaa taaaatgact
    17461 tagagcatga aactagtaag aaaataattc catacataac aaatagcaaa aaaagattat
    17521 tatcgggtat gcttagagca acaaaaaaat aaccaagata gctaggtaag aaattatcgt
    17581 taacatattc taattctttc acctctcctt gcttgaattc atccttcccc aagtatttgc
    17641 ttagccataa gacagtagcg gttgcgataa atggtattag aaaataaaaa agtagactta
    17701 acttagaaat aggttctatc caatgatttg aaattacacg ataaaaccaa gtaaggtcta
    17761 atatataata ttgcgattta ataaaatata ttgataacag caaagatgta ctacttatat
    17821 ttaataatat tttaaatagt ttatccatct cttgctccgc tacatcttac ggatacctgc
    17881 ccgttcatca gcatgggtag taggaaatcg cgtagttggg tcaggtggtg gttttgtttt
    17941 agattatttc gaactttaac aaagaaagta tttgctattt cattatattt ttgagaaata
    18001 tctttactag gcaaaattat tttaaattct tttaagaatt taaaatgtct agcataacca
    18061 taacttgata aatctatttg atttatgatt tggtaaaaca aatagtttgg cattcgttca
    18121 ttattgctta atattacttg tgtaccatct gctccccgcg catattgaaa attaactaac
    18181 ttaacaattc ttgtatgatc accaaaaata atatgagcat cttgtgggtt taatatagat
    18241 ttttcatcat tggtaaatcc acaaataaaa tcttgacttt ggtcaacaac aggatatttt
    18301 ccaaaatcct taatatcttt atttaatatt tttgtagtat ttgggatttt tgctaagcaa
    18361 gattgtaatt ctatacttcc ccaccccttc ggaatttccc gtttcaaggt ttcgtcaaac
    18421 accatgtcgc cgcctgagga tttgtagggc ttgccgttgg cgtcggggaa atcgaactgc
    18481 acaaaccagt agtcgtacag ggttttcgcc atctcttcca ggcgcgtgtt gatttgtttg
    18541 ttcagggcga tttttttgtc caaagcggat aggacggcgg cgatggattg ttggatttga
    18601 ggttctggaa taggcaactc cagtgttttt aatgcatttt cattgacacg ttgcctgccg
    18661 gaagttcctt ccatacattc aatcgctcgt tttctaaaat cgggactgat tgcaaaataa
    18721 tagagaaatt cgggattggt ttcatttttt gctcttaaca cgataaattc ggtagaaccg
    18781 aaagccactt ctccatcatc taaaatatct acaaaagcgg ttttcccatt ttccaaacac
    18841 ggggtaatct tagctaataa ggtatcacca ttgcgaaact tagctccacc gttaaatgcc
    18901 ttgatttcgt aacccgtaat ttgtcgttga aattctttca acattgccat cggaacactt
    18961 tttgccaatg ctcccttggc tagttgctct ctcggattaa aatcagcaat attttgaatt
    19021 tgttgtttct taaaatgatt catgatgttc cgttacaaaa ctcttcaaag gccgtctgaa
    19081 aaaaacattt catcggtagt tcttgccgtc tgcggcagtt gtaaactgaa agtttacaac
    19141 tgaatcgtcc agttcttgtt ctgcaaatat ggctttgatg tgtttaccga tgttttgttt
    19201 gctggtttgg tagagttcgg caatttcttt ttgggcaagc catagttcgc cgtcaagctc
    19261 gcgcagggta aattcggata gtccgtcttc cgtggtgtag atgatgatgt cgttgttgtg
    19321 catagctttc ctgtatctat aaatggttaa ggctacctga aaatctactt ctcaaatccg
    19381 cccgctttct tttaaatatt cccgtaccaa caggcggttg atggtctgga tgtctgcggg
    19441 ttgaacagga aattgctgtt ccgttctaaa gcgttttaat acgatgccct gcatttgttt
    19501 ttcaaagtaa ttctggttgt ccaaaatctg ctccatatcc agcactcttt ggtcggcgtc
    19561 ggttttgacg ccgtttagcg cgttgaagac tttgagtttg tctccgtaga gggcggcgtt
    19621 ttccatcaac cgcttgtgga tacgtgcgta tttggcgtcg ccgccgtatt tgtgccgcaa
    19681 gaggtcgttt tgtcggttcg gctctttgat tttggtatag acggtttgga gtgtggcaat
    19741 gtttgcctgc atttcttcct gcccgacttc ggcgaggttc tttttcttga agatgcgttc
    19801 cagctcttcg cgcaggctga tgtattcaag gtcgtcttgg tcgaagttgc ccgccagccc
    19861 ttcgcgcact ttgcgcatga tgtcttttaa atcgtcggca agtttgagtt ccgcctcgcc
    19921 gattttgacg aattgaaagt aaacgtcttc cagcgcttca ttgagcaaat gggcggtgtc
    19981 gccttgctgt aatttttcgg caaggtttaa ggtatccaag cgggcggcgg tttcgcggta
    20041 caaaagattg agcttgtcaa aatccagatg cgcaagaaat tcgtggctgc cttgcaggcg
    20101 caggatgttg tacaactctt tggcggtttg cagggctttt tttaaggcca gaagctgttt
    20161 tttgtcttct atttggctaa tttggctgca aaattcttcg gcgttttcgg tatcgaaatc
    20221 aaacagggcg tttttaatgt ctgcaatttc ctgttcgatt tcttcggcgg ttttgaatag
    20281 ctggctgtaa ctgccgattt cgtcgcccaa ttcgttggaa agttcgtccc aataggcgcg
    20341 gttggttttg tcaaattcgc gttcgatatc ggcaaaatcg acgacatagc cgtagcggta
    20401 agatttgtag gtgcggttga cgcgggtcag ggtttgtaaa aggttatggg cttttatcag
    20461 tctgcccaaa tagagctttt tcaggcgcgg cgcgtcaaag ccggtcaaaa gcatgttgta
    20521 cacaaacaag atgtcggttt tgcccgcttt gaaatcttta acccattggt cgcgttcttc
    20581 cttagtgccg acgtcgtgca atatcaacgc ggcggtgaaa ttgtggtctg aggccgtctg
    20641 aaaatgctca aaaagctggc gtgcctgttc tgcgctgtcg caaacgacca tggcaccgag
    20701 gctctcgtct tggttggttt ttctgaattt ggcgaagtcg tccaagatgt agtcaagcat
    20761 cgggtggacg aagtgcggat gggcgtagat ttctttgcgg tcaaagctgc ctttttcgat
    20821 ttcaagttgc gccagtgctt cttgtaattg cgccttgtat cggctgccga tttcttcgcg
    20881 tatcaggcgc agggtgtagc cgtcggcaat ggaggcgttg taatagtatt tgtggatgta
    20941 gtcgccgaag agttcgcggg tgttgacgtt gcctgcggtt acgccgataa gcggcgtgcc
    21001 ggtcagcccg attttgacgg cgtttacgtc ggactgatta aggttggcaa gaaatgagcc
    21061 tttggggttg tagctgcggt gtacttcgtc gagaaaatag acgcgctgaa tggcgaggtc
    21121 gtagtcgtta cgggcgacga catcggggtc gtcttggaat ttttggatgt tgacgacggt
    21181 gatttccggt ttgcctgcgt ggttgtgcag ggtttgggcg gatttgatgt cggcagcgaa
    21241 ggcttcgcgg ctgtcgatgg tatggacgat taagtcgcgg gcggtaaatt cccgctgcgc
    21301 ctgtttcaat aaatcgagcc tgtccacgat gaaatagaat ttcggcacga taccctgttt
    21361 ggcgtaatag tgggtcaggt aacgggtgtt gtaataggca ggcgcggttt tccccgatcc
    21421 ttgggtatgc caaatcacgc cttttttacc gccgttcgat aaatgttttt caatggcaag
    21481 ggtggcaaac agttgcggat aacgcatgat gtgtttttgc accgaacctt gacttgcttt
    21541 gacgtaagtc agcccgtgtt gcaggagaaa ggcaagacgt tcgcggcaaa gcagcgaagt
    21601 caggatgcgg ttggtcggcg tatcgggcga tttattgctg ataaattcgg ggctgtgttt
    21661 gataacgggc agattattgt cttgtagaac gttgttttca aggtcgtctg aaagcgtgtc
    21721 caataattcg gtaaggttta atttatgctc ttcgcggaag tagttgaata cgggcttgcc
    21781 gcaagcagac gaggcgtaaa acgcgccttg tgccggctcg gttgcgccgt cgtcgtattc
    21841 catgttgttg gaaaaaatca tgaattgggt gatgttgata aaacggcgga atttgggatt
    21901 tttcgcacgt ttgcccatgc gctcccgctc ttcgccgatg ccgcctttat tgttgggctt
    21961 tttgacttcg ataaagacca aaggcatgcc gtttaccagc agggcaatgt cggggcggaa
    22021 tgcttcgtcg ccgttgatac aaggcaactc agttacaacg tggaaactgt tgttgtcaaa
    22081 attttggaaa tcgatcagct tcttaccgcc ggattgattg gtgaggcgtt cgtaaaattt
    22141 ttgccccaaa tcttcgttat ccagctctaa gcgtatgtcg gcaagcaggc ggcgtgcatc
    22201 atccggcggc aaatcgggat tgatgcggca gaggctgtct acaaagattt cgggaaagat
    22261 attggtttgt ctgtcccaat ttgcattttt tagcgaaaga taatcatagc ccaagcgcat
    22321 caggtgcaaa acggcgggga ttttgacgcg ggaattttcg ttgtgggttt gggacataaa
    22381 cgtaacgtaa tgggtcatca aaatttattg aaacggatta taacacctga aaaaagccga
    22441 tacgcctttt cggtgtaccg gctttgccat actgttctgc ttcagacgac atcgcttcat
    22501 tttgccttta atgcctcttc gtccagcgat ttcaaccatg ccagcttttc gccgattttg
    22561 atttccagcc cgcgcgggac gggttggtaa aagtccggtt cgtccaagcc gtcgggcata
    22621 taactttcgc cggcggcgta ggcgttcggt tcgtcgtggg cgtagcggta ttcgcgtccg
    22681 tagcccaatt ccttcatcag cttggtcggg gcgttgcgca ggtggacggg cacttcgtcg
    22741 ctggcgtttt ctttgacgaa gcggcgcatt tggttgtatg ccttgtagcc cgcgttggat
    22801 ttggcggcgg cggcaaggta cagcacggct tgcgccagcg cgagttcgcc ttcgggcgag
    22861 cctaagcgtt caaaggtggc ggcggcatcg ttggcgattt ggaaggcgcg cgggtcggca
    22921 agcccgatgt cttcccaagc gatacgcacg atgcggcggg cgagatagcg cgggtcggtg
    22981 ccgccgtcga gcatacggca aaaccaatac agcgcggcgt tcggatgcga accgcgcacg
    23041 gatttgtgca gggcggagat ttggttgtag aaactctcgc cgcctttgtc gaaacggcgg
    23101 atttgcgcgc ccaagctgtc ggcgagaaat tcggtggtca ggattttcag acggcgcgtg
    23161 tcggcggcac gtaaaagttg ttccaacaaa ttcaataatc tgcgcgcatc accgtcggcg
    23221 gtattgacga gcagctcttg cacatcggct tcaatcgtga aatcccggta ttcgggcaaa
    23281 gccaacactt tggcaatcag tttcttgagg tcgtctgaag acaaggattg caaaacatat
    23341 acctgcgcgc ggctcagcag cgcgggattg acttcaaacg acggattttc cgtcgttgcg
    23401 ccgataaagg tcagcaaacc gctttcgaca tgcggcaaaa acgcgtcctg ctgcgccttg
    23461 ttgaagcggt ggacttcatc gacaaacaaa atcgtcgcgc gtccctgctg caaagcgatt
    23521 tcagccttat cgattgcccc gcggatgtcc ttcacgccgg aaaacacggc ggaaacaggc
    23581 aaaaactggg cattgaaact ctgcgccaaa atccgcgcca acgttgtctt gcccacgccc
    23641 ggcggccccc acagcaacat agaatgcggc ttcccgcctt ccaccgccac gcgcagcggc
    23701 ttgccttcgc cgattaaatg ctgctgcccg ataacatcat caagcgtatg cgggcgcaga
    23761 cgttcggcaa gcggtgcgtc gggttctcgg acaaacaaat cggtcataac ggctccgtca
    23821 acaggttttc agacaatatg attatacggc agggaaaggc ggcgtgccgc atacggatac
    23881 cgccccaccg tttgtactaa gccgatctta ggcgtacaat tgggaaattt caggaaattt
    23941 atacgctatg ccatccatgc agctttgcaa ccggacccgc aaagaccagg tcccgtttta
    24001 accatgcggg gaagttaaga ttacagtaaa caaaactatt ttgccttatt ggaagaaagg
    24061 ggaacagtta tgagaaattg gaacttatta ccaccgggga aggggcagat ggagacttac
    24121 tggtcaacgg ttcttacggc atccgttctt ttgcagacct aaaattggaa tacaaacccg
    24181 aattatcggt gagaagcaat catgacatta ttttcaacaa tgattttcaa tctgttgacg
    24241 tacaacccta ttccggaaat atccatttgg atgccaaagg aaaaattcaa tttaatttga
    24301 atgttgccga taaggtaaca gatccaacca cacatcaaga aaagttagtg aacgataacg
    24361 ctttagtgcg gtatgtcaac aatatcaccc cctccgatga aaatgacaaa ggcagcatat
    24421 ccttccattc aaaaacaggc aatttgttca atatgaacgt taacgttcat aaaggcacac
    24481 accatttcgg tattcacaac ctccgcaata ccaatgttaa tctgactgcc gaagacggcg
    24541 ataatgtggt tgcgattaaa gggaagcttg ccgagcctat gccggcagga aaagttgaga
    24601 gtacaagttt tttaaggggt attgaaacgg cttttaaatc caataccgta ttaacgggga
    24661 ataacaataa aatatccatc accactgagg gcgacggctt gggattgggc gtaaatggcc
    24721 tccagtccaa ctccccctat tccggacaca cgacgggtac tttcatcaat aatcaaccaa
    24781 gcaatactac ccaaccttcc cttgacggca ccagcaccat cgaactcaaa gccgtcaagg
    24841 ggaataatga agttgattta aatgtaaaaa accacgcctc cgtaaaaggc attatcacgt
    24901 cgcattctgc aaaagcaacg cttgaagccg aggaggataa tattgtccgg gttaaaaatc
    24961 cggacaccaa gacggcttta atcaatgcct ggaaagaaaa ataccccaat aattcgccag
    25021 aaccatacag ggtggggggt tcaatccacg attgacagcg atgtagtttt gagtgcggca
    25081 aacaatatcg ttgaagtagg aggcagcccg atgaaccaag aagggattac cgctcacgga
    25141 aatgccacga ttaccctcaa ggcgaaagaa aacaataaaa ttaccgtgga aaacgccgca
    25201 tacagcagcg acggcatttc gactctgatt aacagaacgg gggcaagacc cggaacaagg
    25261 gatgatggaa ataaaatcat actggaagcc ggcggcgata atattgttac catgaaatcc
    25321 ggcgatgcgg atgcggatta tgtaaacaat tccaaagtat taacggagac accatattat
    25381 aaaagcaaac gaggttccaa cggcattttt gcctatggcg acaaatcgct ggtcaaactg
    25441 attggcgaga ataatatcgt taagagtgaa atcagtgaaa aatctaaggc attaaatggg
    25501 ggatttcgcc atatcggcat ttattcatgg caaaacgcga aagtcgaatt gtctgcgaag
    25561 agcgacaata tcgtacaagg cggaatttgg ggcttatact ccaacaactc ctcaatttcc
    25621 ctcaagggga aaaaataatg tgatttcaaa cccgaaatat aatgttttcg cctacaaaaa
    25681 ggcaaaggtg gatttggtca aattaccatt gactccaacg ttgccaatct tgcaaggcaa
    25741 gatggttcaa ttcatttgaa ttataaagac gatacccgta tcacaggggc aaccgtatct
    25801 gataagggtt tggtagccat caaacctttg aataacacga atattgttgc cgacactatt
    25861 cactataaag gcgatgtctt ggcggtaaat aagggtaaag tggaattaga tttcacgccg
    25921 aacatccgtt tagtgggacg tttggataat tttagcggct taaccgattc caaacataaa
    25981 aatttattcg aaaactatgt tgcaaattta gacagcaaaa gtgcgggcga aattaacttt
    26041 aatttagcca aagacgcatt atggacgatg acaggtcaaa gctggctgga taaattggaa
    26101 ggacaaggca ctatcgattt taataatgat gctaaaacaa gtggacgcgc cttacatatc
    26161 ggtgaattgg cgggtgccaa taaattcttg atgcatctga ataaagacgg cattcacagc
    26221 gatatgctct atgtgaaaaa aggcacttcg acaccgcaag aagtcgtcgt caaaaatctg
    26281 tccgaagtgc tcgacagtat gaattacggc gaacgtttgc gtttcgctac agtaacaaac
    26341 tcaaaaaatg aatttgtgaa cggtaaaaaa tatattgacg atacgcacct tatggaggat
    26401 gccctgactg tcgaatactc cgcacataac ggcgataaaa acaacaagga tgactataat
    26461 aaatccttta acggctctga aatgacggcg gaaaaagctg gagacgatta tgtcaataaa
    26521 acctataccg acaacaggca aaatgtctat ttggtcaaac aggctaccgg caatccgagc
    26581 cgaaatgtca aaaatatcaa tgatatgttc gattcaaccg cacattatgc gttcactttg
    26641 gatacttatg ccaaacgcga aggggagcgg gctttttcaa cgttggataa aaaagaaggc
    26701 gattggataa ggctgacgca tacccgtgtg attcaatcca atgcgtttag gtttcataac
    26761 aacgattttg aaatcggata tgaccgattc agcctcaacg agcaggagaa aaaacgcaaa
    26821 tggggcataa gtctcgacta cggccacggc aggacatcat tatggaatac gtttggcaag
    26881 gacaaaatca ggaaatatga attggctctg tacaatacta cccaatacat agataaagaa
    26941 ggagacgaaa cagggtatat cgacaatgta ttaaaaatag gaaaactgcg taaccgtgtg
    27001 attgcacgaa atcatatggg gcaattatgg ggcaagggaa aatatagcaa caccctattc
    27061 tctatcagca ccgaatacgg ccgccgtaaa tttttggatg acgataaatt gtggcggatt
    27121 acaccgcaag tacagttgca atattcctat ttgagaggta ccggctatcg gatcgataac
    27181 ggcataaacg tcaatttaag ccacgcaaac agcctgatag gccgcttggg tttggatgtc
    27241 gtgagaaaat ttgacggagg caaaaaactt ttctatatca aaggcaatat ctttcatgaa
    27301 tttttgggca gtcgttcctt taaggcattt gagggcaaaa gtcattatgc tcaaaaatga
    27361 aatacgaggg ggacttggta ttccgccgga ttgggctaca atgcgcggac aggcaaaaaa
    27421 aacgaatgtc tttgccgatg cggaaaaaag agttttccgg cgggaaaaaa ggatcgtaca
    27481 acatccgctt atccgtttcc catcagtttg actgaatgat atcggggccg cgccattatt
    27541 cggcaggccc cattttattg ccacacattg cctgcccgat tcccgtttca acagcaaacc
    27601 cttccaatat tttgttcaaa cttggatcgc ggcagatggg ggcgtgcaag ccccgcaagg
    27661 tttttttggc ttttacggaa cagcacgcat ttcgccaaac ggcaaaattg cattaacgga
    27721 attgaaaact tttggaattg tgcaaaacgg catttgcgtc agtttaacgg catttccaaa
    27781 gggcatttta agccgtattc gagagaatgc ggatggcgtt ttcagtgttt tcagcgtgat
    27841 aaaattcaaa tttctattct aaaacaatca gcaaagcagg atttatttca gttgtccgag
    27901 acaacgggcg gctgcaccgc ctgcactccc catacttttt caacgcactc gagcaggagt
    27961 gagacgtggg gattatcctc gtattcttcc gcataaatga aattgagcgg catactgtgg
    28021 cggcacgatt cgataatagc cacgcccgcg ccttcttttg ccgccgcttc ggctttgttt
    28081 ccctgcacca ttgccacacc tataccgcct gcaaccaaat cgataagggt ttgggggtag
    28141 tcgcacaata tctgtttttt tgggtgagag ccggttgctg cgccaaaact ggtgcaggtg
    28201 cttcctactt ccggacacgc ccgacatttc tatccatacg cattcttgca ggctctgcgg
    28261 aagggagcgg gtcagccggg aatattggct ttgcgggcaa atcagcgcgt aggtcaggtt
    28321 ttgcaggaat acgctgcgga tgccgccttg ggcggcgttg ccgagtataa agccgccgtg
    28381 cagggttttg tgttggatgc gcgagaggat ttcgccgctc attccgtatt ggatgtgcag
    28441 gcgcgttttg gggggctgtt tgaccgatat tgtccgtcag cgcgacgagt tttgccgaat
    28501 cgatggggtg gataatgccc aaactggtct cttccgaata atcgcctgcc agcgttttgg
    28561 caaaatactc caacttgtgt ttgtattgca gcagggattc cgcttcgggc aacagtattt
    28621 cgcccgcccg cgtcaatacc atgcctttcc ccgtgctcct gaacagcggc gtgccgacat
    28681 attcttcaag ggctttaatt tgggcggaaa cggcgggctg ggaaaggaaa agtcgtttgg
    28741 cggtttgggt aaggttgccc tcgtgcgcga cggcgacaaa tgattttaat tgtacggcat
    28801 ccatgtatcc ctccttgtgc ggatgttttc tatatttgtg caatcgaaat cttttaggcg
    28861 gattgttgct gaaaattaac tttttaatca agtggtttgt aaattgtatc agttttccgg
    28921 atgatgatta ttaaaaaaaa attggtttta ttgcctgttt gggctttaaa tggggttacg
    28981 gcttccgaac gcagcccgta tcaaaaagaa aagtcatgcg ccccctttta cgaggcgcga
    29041 tatataagga ggaaggttat ggaaaaacat aatgggactt atcgggattt acaccgtccg
    29101 gcttcggaat ttgcgacgcg ggacgaatat ttggaacatg aattgcagat tatgcaacca
    29161 aaacgctggc ggcccaacct gccctttcgc gattaccgct tcgagtggga ggatttgatt
    29221 cctgcgatgg cgggaacgat tggaaaagtg gtgatggtgg gggggcggcg gcgtttgccg
    29281 cacctttggg gctgcctgac agctttgtac tggaaaatgt gcgctatgag cttttaatcg
    29341 ccgccgcgtt tatcttattg gtatcgggct tttttctgcc cggcgccaac ctgcccggta
    29401 cgcacgggcc gctgattccg atgattccca tcgttgtgtc ggcaggcggg catcctttgg
    29461 cgttcggcat ttcgattgcg gttttaggtc tgctgatggc tttatttcgc ggcggcagta
    29521 ttatggcgaa gctgacaagc aacggcgtat gcggcggatt attactctat ttgggcttta
    29581 tcggcacgac ggggcaggta aaaaaattgt tttcgtgggc aggcggtttt aatatgccct
    29641 acatcgcttt taccgtcatt attgtaacga ttgtgatgta cgctttgttg gagcattgga
    29701 aaaaacgctg gttggccgtg cctttgggat gcttgattgc cggtgtggcg gcatttgcat
    29761 tgggtgcgcc gtttgagttt cacaccgccc cggcctgcct ccaatgagtc ctgcttattg
    29821 gtagggtgaa aacagcggct ggcatctggg gttgccgacg gcagaaagtt ttttggttgt
    29881 ctttccattt gcggtattgg ctgttgcaat gtggtcgccc gattttttag gacatcaagt
    29941 gttccaaaaa atcagctatc cggaaaaaac cgataaggta ttgatgaata tagacgacac
    30001 catgacaagt tgttctgtcc gtcaagcagt gggttctatt ttagggggtg caaattttac
    30061 ctcttcttgg ggaacttata tcgtgccggc atcgattgcc aaacgcccca ttccgggcgg
    30121 tgcggtttta acggcggttt tatgtattat cgccgggttg tggggctatc cgatggactt
    30181 ggcgatttgg cagccggtat tgagcgtagc cttgatcgta ggcgtatact taccgctttt
    30241 ggaagcgggc atggaaatga cgcgcaaagg caaaaccacc caatccgccg ccatcgtggt
    30301 gttctcttcc gtctggtcaa tccggttttc ggctgggcgt tgacgatgct gttggataat
    30361 ttgggcttaa tcggctgcaa agaacgcagc gcgcaattag gttttgtcgg acgagtattg
    30421 atacccgcag taggtttctt aatcttgtgt gtggcgatgg gtgcggtcgg gatgctgccc
    30481 ggtatccctc cgtttttgga gcagttcaaa tctttgggct aggctgaaat cgcaaatgcc
    30541 gtctgaaccg gtttcagacg gcatttttgc aaacaggcaa aatgacggcg gcgggatttt
    30601 ttattttccc gattgaagta taatgttgcc gggcttcaac cggatattca aacggtttgc
    30661 tccaacactc ggaacggcgc ataaaacgcc gcccttcgcg ttatcccgaa cggggcggct
    30721 aatcagatcc tatcgccata aaaggcgggg tttcaaccga aaaggaattg agatgaataa
    30781 aaccttgtct attttgccgg cggcaatctt actcggcggg tgcgccgccg gcggcaacac
    30841 attcggcagc ttagacggcg gcacgggtat gggtggcagc atcgtcaaaa tgacggtaga
    30901 aagccaatgc cgtgcggaat tggacaggcg cagcgaatgg cgtttgaccg cgctggcgat
    30961 gagtgccgaa aaacaggcgg aatgggaaaa caagatttgc ggctgcgcta ccgaagaagc
    31021 acctaaccag ctgaccggca acgatgtgat gcagatgctg aaccagtcca cgcgcaatca
    31081 ggcacttgcc gccctgaccg tcaaaacggt ttccgcctgc ttcaaacgcc tgtaccgcta
    31141 accttcttca gacggcatga ggcagatgcc gtctgaatcc gttttcctgc aatttcgaca
    31201 aacgagaaaa atcatgagat acatcagcac gcgcggcgaa accgcacaca aaccgttcag
    31261 cgaagtttta ttgatggggc ttgcacccga cggcggactg atgctgccgg aacattatcc
    31321 gcaaatcggg cgcgaaacct tggacaaatg gcgcggtttg gcttatcccg aattggcgtt
    31381 tgaaatcatg tgcctgttcg ttaccgatat tccggaggac gatttgcgcg acattctgaa
    31441 ccgtacttac acggaagcgg cgttcggtac taaggaaatc acccccgtcc gcacgctttc
    31501 agacggcatc aaaatccaag ccttgtccaa cggcccgacg ctggcgttca aagatatggc
    31561 gatgcagttt ttgggcaatg cgtttgaata tgttttaaac aaagaaggca aaaaactcaa
    31621 tatcttgggc gcaaccagcg gcgatacggg ttcggctgcg gaatatgcct tgcgcggcaa
    31681 aaaaggcgtg aacgtattta tgttgtcgcc cgacggtaaa atgagcgcgt tccaacgcgc
    31741 gcagatgtac agcctgcaag acgggaatat ccacaatatc gccgtgaagg ggatgtttga
    31801 cgactgccag gacattgtga aggcagtgca gaacgatgcc gcgttcaagg aaaaatacca
    31861 tatcggtacg gtcaattcga tcaactgggg gcgcatcgtc gcgcaagtgg tttattactt
    31921 tgcaggctat ttcaatgcca cttcaagcaa tgacgaaacc gtcagctttt gcgtaccgag
    31981 cggcaacttc ggcaacgttt gcgcgggaca catcgccaaa caaatgggcc tgcctgtccg
    32041 ctgcctgatt gtcgcgacca atgaaaacga tgtgctggac gagtttttca aaaccggcgc
    32101 ataccgcccg cgcaacagcg cgcatactta tgtaacctcc agcccgtcta tggacatttc
    32161 caaagcgtcc aacttcgagc gtttcgtgtt cgacctgatg gatcgcgatc ctcaggaaat
    32221 caatacgctg tgggcggaag tcgcggcagg caaaggcttt gacctgcggt ttgccttgga
    32281 caaagtcggc ggcaaatacg gctttacctc cggcaaatct acccacgccg accgcctcgc
    32341 caccatcaga caggtttacg agcaagacaa agaactcatc gacccgcata ctgccaacgg
    32401 cgtaaaagtc gcccgcgaag tgcgcgaagc gggggaaacg gtggtttgtt tggaaaccgc
    32461 gttggcggcg aaattcgatg cgaccatacg cgaagccgtc ggcgatgtcg ccattccgcg
    32521 ccccgccgcg ctggaaggtt tggaaaacct gccgcagcgc gtgcaaaccg tgccgaacag
    32581 tgcggatgcg gtaaaaggca tcatcgaaca aacccttgcc tgatgttccg tatgggcggc
    32641 attccatccg aacttaagat tgaaagaaaa tgaccttacc tatgcaggaa acccgttttt
    32701 ctatactgct ggacgaattg gcggcaaaac aggaggcaac catcgccccc tacctgcttg
    32761 ccgacgggac caaggtatgg gtacgcaaag caggcaggca caatgcgcga tggcgttacg
    32821 cgctgctcgg tatggttgcc cgatatttga aactgggggt gttgaagccg gttcccagcc
    32881 tcggcggcga gcctgccatt gcaaccgaat caaaacgcct gtacgaattg cgttcggcag
    32941 ggatagccgt tcccgaattg ctcgccgtcc ggaaaaatgc ccttatgttc ggcaatttgg
    33001 aaggcatccc gctcgatacg caaatccgcc aagaagccga agccggaaag gcggacgctt
    33061 ggcttgccgg tttggaagcc attgcacgcg tgcataaaaa acggcagttc ctcagtcagg
    33121 cgtttgcgcg gaatatgatg tgggacggaa aaaacatcag ctttttggat tttgaagacg
    33181 acccttccga ggtcctgacc atagcgcaat gccaagcccg cgattggctc tgctacatcc
    33241 attcgaccgc gctgatattg aaaaacggcg gactgctgga agcggctgcg gaaaaatggg
    33301 gcggcgtatt gtcggatcag cctgccgaaa tacaaaagct gattgccggc accgtcaaac
    33361 cgatcctccc gatacgcagg ctggaacacc cccgctgggg acgggacgcg ctcaggctgg
    33421 cagcctcgat ttccttaatt tccctcgccg atatgccgcc gtaagccacc gcccgcccca
    33481 cgatgccgtc cgaagccctt cagacggcat ataaccaacg cgcatcaggc attgtcgcaa
    33541 ccatccgttt cgtttagaat tttgacttta tcccgaatcg ggcagacatt tccactctgc
    33601 cgcacgtccc gaacaagcac tatccatttc ttttactgac aggaaaccca aatggcagca
    33661 ttcaataccc aaaaagtatt gtccgtacac cactggacag acgcatattt tacctttacc
    33721 tgcatccgcg acgaatcgtt gcgctttgaa aacggacagt tcgtcatggt cgggctgatg
    33781 gcggacggca agccgctgat gcgcgcatac agcgtcgcct ccgccaactg ggaagaacac
    33841 ctcgaatttt tcagcattaa agtccaagac ggcccgctga ccagccgcct gcaacacctc
    33901 aaagtcggcg acgaagtgtt aatcagcaaa aaaccgaccg gaactctagt tgcctgcgat
    33961 ctgaatccgg gcaaacacct ttacctgttg agtaccggta ccggcatcgc ccctttcttg
    34021 agcatcacca aagaccccga aatttacgaa cagtttgaaa aaatcatcct tgtacacggc
    34081 gtgcgctaca aaaaggattt ggcatactac gaccgcttta ccaaagaatt gcccgaacac
    34141 gaatacctcg gcgacttggt taaagaaaaa ctgatttact acccgattgt ttcccgcgaa
    34201 gaattcgaac accgcggccg cctgaccgac ctgatggtaa gcggcaaact gtttgaagac
    34261 atcggcctgc ccaaaatcaa cccccaagac gaccgtgcca tgctgtgcgg cagccctgcg
    34321 atgctgaaag acacctgcaa agttttggac gatttcggtc tgacagtctc cccgaaaacc
    34381 ggcgtgcgcg gcgactacct gattgagcgc gcgtttgtgg atcaataagc cgcttgaaaa
    34441 tgccgtctga acggcatcag cgataaaaaa acccgaatcc tgccaatcag gattcggggt
    34501 ttttatagtg ggtaaaaatt agaaatgcac atttccgtca ttccaacaca ggtgggaatc
    34561 cggacttacc tgtttcatta tcgtttgaaa ataaaagaga aaccaaacat atagattccc
    34621 gcttgcgtgg gaatgacgac ggtgttttgc cgtagccaaa gcaggaaaac atccttattg
    34681 tcatttcatc atgataggac agtaaggatg ttaaaaatta attatcatta tatgattgat
    34741 atataataaa aattatccca ataagaaatg ttatgcagaa aataaaatga aaaggatggt
    34801 tttcattgaa aatggttgtt ctgccaccgt catacacaga accggtattt ggaaaaaaaa
    34861 ataatccata ttgacatcat tattccattt atcgcataaa tccacttttt aatttttttc
    34921 ctcattaata taatctctta tttaataaaa tcaaatacct agcaattcga tctcaaattt
    34981 taaacgccaa cgtgtttgag cggtaacaat gcatacttta agtttgtaat ccttcatcgt
    35041 ctcatttttg ttttaatcga ctatacgtct aaagcaacgc cgcccgtcat tgttcggaca
    35101 gtattttttc agacggcatt ttcccccgtt tgtgaaggcg caaggcattg cccaaaaccg
    35161 ccagcgaact gaaactcata cccagtgcgg ctatccacgg ttggacatag cccaaaacgg
    35221 caagcggtac ggcgatgatg ttgtacgcgc ccgcccatat caggttttgc cggataatat
    35281 ggcgggtgcg ccgcgcctga tcgagcaggt gggcgacggt acgcaaatct tcgttcaata
    35341 acacaatgtc cgcgccgtcc ctcgcaatat ccgtcccgcc cgctgcggcg gcggatacgt
    35401 ctgcctgcgc caaaacgggc gcgtcgttga tgccgtcgcc tatcatcagc acttttttcc
    35461 cttctttttg caaggcttcg acgtattcca gcttgtcttc cggcattgct tgggcgcggt
    35521 agtgcgcgat acccaaggca cgggcggttt cagcaactgc ttcttcacgg tcgccactga
    35581 ggatgtgcag cgtcaggttt ttgcctgcca actgccgcac tgcctccgcc gcgctgtctt
    35641 tgagcgggtc ttgcaggtag aacacggctt ggaaaccgct ttgactgccg aggtaaaccg
    35701 cgctgccgcc gccttctgtt tgcggttctt tacctgaaat ttcggcaaca taggatgccc
    35761 tccccaatgc ccaaacctgt gtttcgccgt tgacggtcag ttgcgcaccc acgccttccc
    35821 cgatgcggtt gaggcgttgt ccgacttgga tttcggggac gctgccgcct gaaatgcggc
    35881 agttgagaat ggcgcgcgcg agcgggtgtt cggactgctg ttccaaactt tgcgccactg
    35941 ccagcacttg ggcttcggtc atgctgccga gcagttcaat ccggcggacg gcgggattgc
    36001 cttgggtcag cgtgccggtt ttgtcgaaaa tgatgtcggt ggtttgggag agggtttcga
    36061 ttgcctgctt tccgccgatt aaaatacctt cgcgcgccag cgtaccggta gaagctgcca
    36121 gcgcggtcgg cgtggcaagc gacagggcgc aggggcaggt aatgaccagc agggcgacgg
    36181 taatccacaa tgcggtgtgc gcatcggcgt acagcgtcca gccgatgaag acggggacgg
    36241 caagcaggag ctcgccgaat atgaaagatg aggcgtattg ttccgccaac tcggcagtac
    36301 gcggtttttg cgccaaggcg cggtcgagca ggcggacgat gtgcgacagg cgcgtgccgc
    36361 cgccggtgcg gtcggtgcgt ataatcagag ggctttgcgt gttgagtgta cctgcggtta
    36421 ctttttcaga cggcattttg gcgacgggca ggctctcgcc ggtcagcata gattcgttga
    36481 cggcactgtt tccttccagc accgtgccgt caacggggat ggtttcgccg ggtttgacca
    36541 tcacaatatc gcctgcttgg agtttgacga cggcggattc gcggacatcc tgtacggcag
    36601 gatagccggg catccggtgg cagaaggcgg gtatcagttt caccagcctt tcggcggcat
    36661 cgcctgcctt gcgtcgggca atgtgttcca taaagcgtcc gcccagcagg aaaaacagca
    36721 gcatcgcgat ggattcgaaa tacatcccct gccctgcgtt ggtggcgagg ctgtaaatgc
    36781 cggcgatgaa ggtcatgatg atggcggcgg caatcggcgt gtccataccg gcgcggcggt
    36841 ttttcaggtc gcgcaacgcg ccttgataaa acgggacggc gcaataaaat acgacgggca
    36901 gcaccatcag gaagccgccc caatggagga tctgcaggaa atcgggttcg atgtcgccgc
    36961 cgtaaaggta ggtcggcagc gcgaacatca tcgtctgcat catacccagt ccggcaacgg
    37021 caaggcggac gatgtattgt ttgcgttctt tttggttggc ggcttcgatt ttttgcgcgt
    37081 catagggtgc ggcggtgtag cctgtctgcc ggattttcaa cagaatgtcg gagaggcgga
    37141 ttttgccgtc gtcccagacg acgcggcagc ggtgcgtgct gtaattgagg tcgatgcgga
    37201 cgatgccgtc tgtgcgcaaa agctgctgtt cgatcagcca gacgcaggcg gcgcaggtaa
    37261 tgccgctgag catcagcact gcttcgtgcg tgccattatg ggtttccaca aagtcggatt
    37321 ggacttcggg caggtcgtac aggcggattt ggtcgaggat ttcttggggc ggcagttcgg
    37381 tttttttcgc gtcggcggtg cgtcgtttgt aataactgcc caagccggaa tcgatgatgc
    37441 tttgtgcgac tgcctgacag ccgacgcagc aggtttcgcg gtcttcgcct tcgtagcgga
    37501 cggtcagatg caggttttcg ggaacgtcca gcccgcagtg gaaacaggtt tttttcatgg
    37561 catttcggtt tcgtctgtgt ttggtgcggc ggcacaatac tcggcaatgg cttcgcgcag
    37621 tgcgtctata ccggtatttt cagcaacgga aatgcggacg gcggcaattt ttcccgcagc
    37681 gtcgcgccat atgcccgtgt tttgttcttc agacggcagc aggtcggttt tgttgtacac
    37741 cttgatgcac ggaatatcgt gggcatggat ttcttgcagt acgttttcca cgtcttcaat
    37801 ctgctgtccg ctgttcgggg cagcggcatc gacgacgtgc agcagcacat cggcttgcgc
    37861 ggtttcttcc agcgtggcgg aaaatgccga aatcagtttg tgcggcagat cgctgacgaa
    37921 tccgacggta tcggtcagga taatgctgca tgcgggactg atgtacagcc gccgcgccgt
    37981 cgtgtcgaga gtggcgaaaa gctggtcttt cgcatatatg cccgacttgg tcagccggtt
    38041 gaacaggctg gatttgccga cattggtata gccgaccagc gcaaacgttt tgattctgcc
    38101 cgactcgcgg gacttgcggc gcagggcgcg ctgttttttg aggttggcaa gctgtttttt
    38161 caaggcgttg atccgatggg cggttaatcg gcggtcggtt tccagtttgg tttcgcccgg
    38221 ccctttcatg ccgataccgc cgcgctggct ttgcaaatgt ccgtaaccgc gtatcaagcg
    38281 tcccgccaaa tggctcaatt gcgccaactc gacttgcagc ctgccttcct gcgtgcgggc
    38341 gcggcgggcg aaaatcgcca gaatcagccc cactctgtcc aatacgcggc attggaggat
    38401 tttttccaaa ttgcgttcct gcgtgggagt aagttcgtgg ttgaatacga ccaaatcaat
    38461 gccgtctgcg gcaactgctt ccgacagctc cgccgccttg cccgtgccga caaacagcgc
    38521 agtgtgcggg cggtcgcgtt tggcagtctc cacgcgtacg gaatcgccgc ccgccgcttt
    38581 gaccagctcg acggcttccg ccaatgccgt ctgaaaaccg ttcagacggg cggcattgct
    38641 gcccgtatca tctttatcca acattacgcc caccagcatg acgcgttccg gttgcgcctg
    38701 agtggcactg ttccgtcctg ttctacctga cataactgtc aatcctgcaa ataaagggtt
    38761 acaccgcgtt ccgggctgtc ttccggagta agcctgatgc ggcttcccgc cattccgctg
    38821 acgctgaaga caatgccgcc cgcttcgtgc cggagtatgg acaggctgtt gccgaactgc
    38881 cgccggtagg tataacgggt acacgcttcc agacaccagc gtccgcgcca gtggatgaaa
    38941 tatttttcgc cttttctaac cgcaagtgtc tcgccgccgt ttacctcaaa ataagggtaa
    39001 tcgggttttc gggttctaaa ctgccatttc gcctgcgcgc gccgtccgtt gcggacatag
    39061 tcgaataccg ccgtataaag cgtgccgtat tccaaaggtt tgagcgggaa aagggcgaat
    39121 tggtgcgcgg tcagcctgcc gttagggtcg ttgccggcgg ttaaaaccct gacggggcgg
    39181 atttcgtttt taccctgata cagcttgaaa cttttcatcg caattttgcc tgccgcctcg
    39241 gaaaaatcaa tgctggcagg attgcctgtg atttcatatt ccggcacggg gtcgggacgt
    39301 tccccgtaaa aataaggcag cgcgccgccg ccaaccggat aggcggtata aagcaattcc
    39361 gttacgggca tggcttcgtc agcataaacg gccgcaccgt tgtggcaagc gttgcggtaa
    39421 tatttccgtc ctgcttccgg ctgccgcctt ccttttgcac aggcgcgctc gaagctgccg
    39481 ttgccctgat tgaatatgag gacggttttg ccgttttcgc gcacaaatgc cgcacctgct
    39541 tcgtcggtat ggcggtcaag cagcgaaagg cggtgataga cagcactcat caaagcgtcc
    39601 acttggcgtt gctgcgtgcg gatgtcgctg tcggacgatt cggcggcttc ctcttccgtg
    39661 ctgatgtttt catgcacgcc gttgtagaga tacccggcaa ggcgtgtgcg ttcggtcagc
    39721 ttttgtgcgg tgtagtgcgg attgtcggga tggtgttcgc cgtgtccgtc ttcgggattg
    39781 agcgtgagat agcgtgcgtg cctgcgggcg gaattttcca aaaccggcgc gtgtgccagc
    39841 gcgtgcaaac cgatttgtgt gcggatgcgg ttgaggtagt gcagcgcatc gccgccgtcc
    39901 ctgatttgcg gtgcggacgg atagacaagt tcgcccgcgg gcagggattg gttttgggta
    39961 tggtagaaaa cgcctgccgc cgagcccaat agaagcagcc aaataaaaag ggatttcata
    40021 atgccgccct cccgacgcgg caacggggtg ttttatcaaa acggcaaact tgccaatgcg
    40081 gtttccgctt gccgattgcc aaaccggcaa acctttcccg tgccggtgcc gaacccgatg
    40141 ccgtctgaac cttcagacgg catcgggtgt ttatttgtcc actcggacgg gtgcaggttc
    40201 gtattgtgtc gattcgtcgc cgtaatacag cacgccgagt ttgacgggga tgcgtccctg
    40261 cgatttgcgg tgggcgttgg aatcgcgcaa ggaataggcg cagccgcagt attcctgctg
    40321 gtagaagttt tcgcgtttgc tgatttcaat catacgcgcg ccgccgccgc ctttgcgcca
    40381 gttgaagtcc caataggcca catcatcgta aggcgcggcg gcacggtgtc cgcagtcgtt
    40441 gatttgcgcc atatttttcc agcgtgaaat gcccagcgaa ctggtaaaga cgggaaaacc
    40501 gtgttcgtgc gcgtattgcg ccgctttttc aaaacgcata tcgaaacaca tggtgcagcg
    40561 gatgccgcgt tccggctcaa actccatgcc tttggctttg gcaaaccatt ctttgcggtc
    40621 gttttcgtag tcgtcgtctt tatcgatgaa agggatgccg aacttttccg caaagcgcat
    40681 gttttcctct tttcggagca tatactcttt gtgcggatgg atattgggat tgtaaaaata
    40741 aatggtgtag tcgatgccgg aggcaagcat ggcttccatc acttcgccgc tgcacggggc
    40801 gcagcaggaa tgcagcaaga cttttttatg tccgccgggc gggacgagta tggggcggtc
    40861 aatgtcggta acggtaggtt tgttttgtgt ttccattaac ttaacaatat ctgtcgaata
    40921 cggtcaaagc ggctatttta ccagagccgc cgaatgtaaa acagggtgtc agggcatcag
    40981 cccgagctgc ctgaacgaac ggacggtttg cgaggtaacg ataaaatggt cgagcagcga
    41041 aacatcaacc agcgacatgg cctgtgccag ccgccttgtg aacatgatgt cttcctgcga
    41101 aggttcgggc gagccgcccg gatggttgtg cgcgataatc aggctgtcgg catattcgtc
    41161 caatgccagt ttgacgattt cgcggatgta aatcgtgttt tccgcaaccg taccgcgcga
    41221 cagctctctg accgcaatca gttggttttg gcggttcagc agcagcgcga cgctgacttc
    41281 gactttttcc tgccccaaat gaaagcgtaa ataatcggca acggtatcgg gatcggataa
    41341 ggtaatttct tcctgcaatt cttcctccaa tatccgccgc ccgatttccc tgaccacggc
    41401 gaactgtgtg aaacttgccg tccccatccc tttgtatgcc gacagtttgc cgacctccgc
    41461 gctcatcagc ctccccaagc tgccgaactc ctgcaatagg tagcgcgcca aatcgaccgc
    41521 gctcatcccg cgcgtcccga cgcgtaaaag gattgccaaa agttcggcat cgctcaaagc
    41581 cgccgccccg cgttccaaca gcttttccct gggcctttcg ccttccggcc attgcttgat
    41641 gctcatgttt tccctttttt aaatagtctg agatgccgtc tgaaacagat gttcccacac
    41701 gccaaatatc cgaaatgccg tctgaaccgg atttccgctt cagacggcat ctgtatttca
    41761 aaacagtctt acgccgtttc ttccaactcg attgaagccg ccaaaagcga cagacgtgcc
    41821 attacgccgt aaacgtagag gcggttatgt tcgttgtcca cgtcgcagtc tttttcagaa
    41881 cgcggcatac cgagcgaatc ccattgttcg aatacgcgtt tgcaggcttc tggagcttct
    41941 tgcgaattat cgccgttacc ggtaggaatg ctgttggaca gcggcacaaa caccataccg
    42001 ccggcgttga ggttttcgtc cgcaccgcgc ccttcgtgta cgcggaaaaa gccgccgatg
    42061 acgaaacggt ccatcatata gacgacgggt tcgcacaccg cgccgttcaa ggtttcatag
    42121 gtataaaccc cttcttggac aatcacttcg ctgacttcca agccctcttt caccttcgcc
    42181 attttgttgc ggtttttgcg gttcaagccg cgcacttcgt cggaggattt gacgctcatc
    42241 acacccatgc cgtaagtgcc ggcatcggct ttgacgatga cgaaaggttt gtcggcaatg
    42301 cccgattcgt cgtatttggc ttgaattttc gccagcacgc gttctaccgc ttccgccagc
    42361 gcatcttcac cttcgcgccc ttggaagtcc aaaccgccga ttttttcaaa atacgggttg
    42421 atctgccatt catcgatgtc aatcagcttg gcaaattcgg cggcaacttg gttgtacgcg
    42481 ccgaaatgat ttgttttgcg gcgcgtcgtc caaccgccgt gcagcggcgg caaaacggtt
    42541 tggccgatgc ctttgagaat gtcgggaatg ccggcggaca aatcgttgtt caacaaaacc
    42601 acgcaaggcg aaaagccgtc ggcaaggtgg acgcggtcgc gggttcgcag caaaggctca
    42661 agcaggattt tgtcgcccaa tgcagtttca aattcggccg gttcggttac ttccggattc
    42721 aggctgccca agcgcacttc acaccctgcc gaacggaaaa tctcgccgag ggcgtaaacg
    42781 ttttgcaggt aaaacgtatt gcgcgtgtgg ttttcgggaa taatcaatac ggatttctcc
    42841 gtttcgcagg cgcgttgcac cgcatcttgc gcggcaaccg ccgccagcgg gatgaaattc
    42901 ggattcagat tattgaagcc gcccggaaac aaattcatat cgatagacga aattttgtaa
    42961 ccggcgttgc gtatatcgac cgaaccgtaa aacggcgggc ggtgcgcgtt ccactgcgtg
    43021 cggaaccacg cctcgatttt ggcgtgattg gacaggattt ttgcctcaaa cgcctgaagt
    43081 tgcgccgaat gttcgggcga cataaccggt aatttcatct ttcgtctgcc tctgtgtgtt
    43141 ttttgacaag gtacagataa taatgctttt gaaagaatga cgacaagtat tctttcgtat
    43201 cgaatcattc tttagatata acgaaatgaa caatatgtct aattttttcg ttaaaattgt
    43261 ttacaaaata acaccgactc aaaaattaga caaaatctgt tgcgcagtat aaagaatacg
    43321 tctaaaatcc gcccaatccg catcaccatt tatccaaaag aacaacatca tgacggcaca
    43381 aaccctctac gacaaactct ggaacagcca cgtcgtccgc gaagaaggag acggcaccgt
    43441 cctgctttac atcgaccgcc atctggttca cgaagtaacc agcccgcagg cgtttgaagg
    43501 tctgaaaatg gcggggcgca agctgtggcg catcgacagc gtggtctcca ccgccgacca
    43561 caacaccccg accggcgatt gggacaaagg catccaagac ccgatttcca agctgcaagt
    43621 cgatacgttg gatcaaaaca tcaaagagtt tggcgcactc gcctacttcc cgtttatgga
    43681 caaaggccag ggcatcgtac acgttatggg gcccgaacaa ggcgcgaccc tgcccgggat
    43741 gaccgtcgtc tgcggcgatt cgcacacttc cacccacggc gcattcggcg cactggcaca
    43801 cggcatcggc acttccgaag tcgaacacac gatggcgacc cagtgcatta ccgcgaaaaa
    43861 atccaaatct atgctgattg ccgttgacgg caaattaaaa gcgggcgtta ccgccaaaga
    43921 cgtggcgctc tacatcatcg ggcaaatcgg cacggcgggc ggcacgggtt acgccgtcga
    43981 gttcggcggc gaagccatcc gcagcctttc gatggaaggc aggatgaccc tgtgcaatat
    44041 ggcgattgag gcaggcgcgc gttccggtat ggttgccgtc gatcaaacca ccatcgacta
    44101 tgtcaaaggc aagcccttcg cacccgaagg cgaagcttgg gacaaagccg tcgagtactg
    44161 gcgcacgctg gtgtctgacg aaggcgcggt ttttgataaa gaataccgtt tcaacgccga
    44221 agacatcgaa ccgcaagtta cttggggcac gtcgcccgaa atggttttaa acatcggcgg
    44281 caaagtgccg aatcccgccg aagaaaccga tccggtcaaa cgcagcggca tagagcgcgc
    44341 actcgaatat atgggtttga aggccggtac gccgctgaac gaaatccctg tcgatatcgt
    44401 ctttatcggc tcttgcacca acagccgcat cgaagacttg cgcgaagccg ctgctatcgc
    44461 caaaggccat aaaaaagccg gcaacgtaca gcgcgtgtta atcgtccccg gctccggttt
    44521 ggttaaagaa caagccgaaa aagaaggctt ggacaaaatt ttcatcgaag ccggttttga
    44581 atggcgcgaa ccgggctgtt cgatgtgcct tgcgatgaat gccgaccgcc tcgcgccgag
    44641 gcaacgctgc gcctccacgt ccaaccgcaa ttttgaaggc cgccaaggca acggcgggcg
    44701 cacccacctc gtcagccccg ctatggccgc cgccgccgcc gttaccggac actttaccga
    44761 catccgcacg atggcgtaag tattttcagg cagtctttaa gaatttgacg aggccgtctg
    44821 aaatatgtcc cgtaaggcag aaacacctaa tattcaaacc aaggagttta accatgaaca
    44881 aacttttcgt taccgccctg tccgccctcg ccttgtccgc ctgcgccggc acttggcagg
    44941 gcgcgaaaca agacaccgcc cgcaaccttg acaaaacaca ggccgccgcc gaacgcgccg
    45001 ccgaacaaac aggcaacgcc gtcgaaaaag gttgggacaa aaccaaagaa gccgtcaaaa
    45061 aaggcggcaa tgccgtcgga cgcggcattt cccatctcgg caaaaaaatc gaaaacgcca
    45121 ccgaataacc gatttgcgat gccgtctgaa accgtttcag acggcatcgt cagcacaaga
    45181 cgcaaaccca tgaaagcctt taccaaaatc accgccatcg tcgccccgct cgaccgcagc
    45241 aacgtcgata ccgatgccat catccccaaa caatttctga aatccatcaa acgcagcggc
    45301 ttcggcccca acgcctttga cgaatggcgt tacctcgacc acggcgaacc gggcatggac
    45361 aacggcaaac gcccgttgaa ccccgatttt tccctgaacc agccgcgtta ccaaggcgcg
    45421 caaatcctgt tgacgcgtaa aaacttcggt tgcggctctt cacgcgaaca tgccccttgg
    45481 gcattggacg actacggctt ccgcgccatt atcgccccca gcttcgccga catcttcttt
    45541 aacaactgct acaaaaacgg ccttttgccc atcgtgttga ccgaagaaca agtcgaccgg
    45601 cttttcaaag aagtcgaagc caacgaaggc tatcggctct ccatcgacct tgccgagcaa
    45661 accctgacca ccccgggcgg cgaaacattc acattcgaca ttaccgaaca ccgcaaacac
    45721 tgcctcttaa acggcttgga cgaaatcgga ctgaccctgc aacacgccga caaaattaaa
    45781 gcctttgaag aaaaacgccg ccaaagccag ccttggctgt ttaacggtta aaacctatgc
    45841 cgtctgaagg ctgttcagac ggcattcctg ataacatata attgctgcgg ctaatgtaaa
    45901 cagaggggcg ataaattgtt ttcatctttt aagcgggctt agcaacactc taaattaatg
    45961 caactctcca tttcaaataa atttttagat ttttaatatg caacaaatta aatttattga
    46021 cttattttcc ggaatgagtg gcatcaggaa aggattcgaa caagcctgtc ggaaacagtc
    46081 ggttgcttgc gaatgtgttt ttacttcaga aatcaaaccg gcagctttgg aagtattgaa
    46141 gcaaaactac cctgatgaag tgccgtatgg agatataacg aaaattgaaa caggggatat
    46201 tcccgatttt gacatcctgt tggcaggatt cccttgtcag gctttttctt tcgccggaaa
    46261 aagattaggc tttgaagata cacggggaac gcttttcttt gatgtggcaa ggattttaaa
    46321 ggcaaaaaaa ccaaaaggtt ttattttgga aaatgtggaa ggattggtga cacacgatag
    46381 aaaagattcg acacaaaaaa tagggcgcac cctgaccgtt attttggaaa ccttggaagc
    46441 attgggctac tatgtttctt ggaaagtttt aaatgcaaaa gatttcggca ttccccaaaa
    46501 caggaagcgc atttatctga caggcagttt gaaatccaaa cccgacttat ctttcgaaac
    46561 aagcccaagt ccgaaattaa aaaatatttt ggaatcggga ctgcctactg aaagcagccc
    46621 tttcatcaaa aaattgctaa aaaaatttcc cccgtccgaa ctgtacggaa aatcagtaaa
    46681 agacaaacgg ggagggaaaa acaatattca cagttgggat attgaattaa aaggcgcagt
    46741 aaccgaagaa gagaagcaat tgttaaatat ccttctaaaa gaacggagga aaaaaaaatg
    46801 ggcttcagaa atcggcatag attggatgga tgggatgcct ttgacaaaag cgcaaatttc
    46861 aactttctat aaacaccccg atctccaaaa tattttggac agcctgacag acaaaggcta
    46921 tttggtttta gagcatccga aacaaaaaat tggcgggcaa agaatcaaag atgaatccct
    46981 gcccaaaggc tacaatattg tttcaggtaa aaaatctttt gaaatcaata aaatattaga
    47041 tccaaacgat gttgcgccaa ccttggttgc aatggatatg gagcaccttt tcgtcgttga
    47101 caacggcggt ttgcgtacac ttaccggaaa agaagggtta cgcttattcg gctatccgga
    47161 cgattattcg tttgatattc ccaaaaaaga cagatgcgat ttattgggta ataccgttgc
    47221 cgtccctgtg attaaggcgg tatctgaaag acttctgcat actttataaa ccgacaggga
    47281 taaaatgata aaacttactg cacaacaaat atttgataag ttgctggatg aagaaaaaat
    47341 cttatcggcc aatggtcaaa ttagattttt cttaggagat gtggatatta tcgtcaaaca
    47401 aaaagatgtt gtcggcaaca tcattcagga atggctcggc ggatggttga gaaaaaggga
    47461 aattgaattt gatgtttcaa ccaataccca aatgcctccc gatttctttt taaataaaaa
    47521 agaccgcagc agagaattgt tggaagtaaa ggcgttcaac cgaaatgcct gcccgggttt
    47581 tgatattgca gattttaaaa tgtattctga tgaaatcatt cataagccct atatgctgga
    47641 tgtagattat ttaatattcg gttacgatat ggacgacaac ggcaatgtaa ccatcaagga
    47701 tttatggctt aaaaaagtat ggcaaattac cagaagcatg gatgggtggg caattaatct
    47761 tcaagtcaaa aaaggcgtgg tgcataaaat ccgcccgggt gtttggtaca gcataaataa
    47821 aaagaatatg cctatgtttg aatgcttgga agattttgtt tccgcaattg aagaaaccgt
    47881 ttatcaaaac ccggcaacac ggcataacgc atccttatgg aaaaagaaat ttgaggaagc
    47941 ttataaaaaa cactacaacc gatcgatttc cataccccgt tggcacgaaa ttgcacacaa
    48001 atacaaaaag aaataaccct tcaaccatca acccataagg aaacttgaaa tgaccaaaca
    48061 tatcgccatc ctccgaggcg acggcatcgg tcccgaaatc gtcgccgaaa ccgtccgcgt
    48121 actcgacaaa tttatcgccc aaggcttgga tgccgactac gaatacgcgc ctttgggcgg
    48181 cgaagcctac gacgaatacg gccatcctta tccagaattt acgcaaaacc tctgccgcaa
    48241 agccgatgcc gtcctgcttg gtgcagtcgg ttcgccccaa tacgacaatc tcgaccgtcc
    48301 gttgcgcccc gagcgcggct tgttagcaat tcgtaaggat ttgaacctgt ttgccaattt
    48361 gcgtcctgcc gttttatatc ccgaattagc caacgcctcc acgctcaagc ctgaaatcgt
    48421 tgccggtttg gatattttga ttgtgcgcga acttaccggc gatatttatt tcggcgaacc
    48481 gcgcggcatc cgtgttttgg aaaacggcga acacgaaggc tacaacacca tgaaatacag
    48541 cgaaagcgaa atccgccgca tcgcccacgt ggccttccaa tccgcccaaa aacgcagcaa
    48601 aaaagtctgc tccgtaggca aagccaacgt tttggaaacc accgaactgt ggcgcgaaat
    48661 ctttgaagaa atcggcaaac aatatcccga tgtcgagctt tcccatatgt atgtggacaa
    48721 tgccgccatg caactcgtgc gcgcgcccaa acagtttgac gtgattgcca ccggcaacat
    48781 cttcggcgac atcctctccg acgaagcctc catgctgacc ggctccatcg gtatgctgcc
    48841 ttccgcttct ttggacgaaa acggcaaagg cctgtacgaa ccgtctcacg gctccgcccc
    48901 cgacatcgcc ggacaaaaca aagccaaccc gctggccacc atcctctcgc ttgccatgtt
    48961 gctgcgttac agcctgaacg acgaagcgcg cgcgcaacaa gtcgaaaacg ccgtccaaaa
    49021 agtgctgcaa caaggcttgc gtaccggcga tatttacgaa gaaggcacaa aactcgtttc
    49081 ctgctccgaa atgggcgacg cggtactcgc cgccttgtaa aaaa
//
LOCUS       DAWTQS010000006        44110 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.006, whole genome shotgun sequence.
ACCESSION   DAWTQS010000006 DAWTQS010000000
VERSION     DAWTQS010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 44110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44110
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.006"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(45..121)
                     /locus_tag="WNA25_000360"
     tRNA            complement(45..121)
                     /locus_tag="WNA25_000360"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(85..87),aa:Arg,seq:acg)
     gene            complement(232..1905)
                     /gene="recN"
                     /locus_tag="WNA25_000361"
     CDS             complement(232..1905)
                     /gene="recN"
                     /locus_tag="WNA25_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFA9823197.1"
                     /translation="MLLTLSLRDFVIVENLNLDFQSGFTVLTGETGAGKSITLDAIGL
                     LLGDKADYSQVRSGAKEAQLSALFDISHLPALKAELREQGLLDEGGEELSIRRIIDAK
                     GKSRSFINNQAATLAQLKAVGGQLIDIHGQNDHHSLNQEAAQRELLDAFVGGRVQAET
                     VRQLYQNWANAKKALQEAQEHADAVIIERERLEWQFNELNQLDIKQGEWEALSQSHDS
                     LAHSAELLQAAEEVGSKIDGDNGIQRHIYQCQKLLANLQNIEPRFAESLNMLASIEAE
                     LGEISANMRDVAGHSDINPNELAAQEQRMGELMGMARKYRIEPEELPAKLAEIEERLQ
                     SLQAAADLDALEHNVAHNFAEYQEAAHILSAMRHQAAGRLSSETTEHMQHLAMKGARF
                     DIVLLPSSPTAHGLEQVQFQVAANKGNPPRPLNKVASGGELARISLALQVVASQYTQV
                     PTLIFDEVDTGIGGGVAEMVGKALRALGRKHQVLAVTHLPQVASCGENHWRVRKHSEG
                     EQTVSEISILDEIQRIEEVARMLGGEVITDTTRQHAAELLQLASKNSLF"
     gene            complement(2089..2691)
                     /locus_tag="WNA25_000362"
     CDS             complement(2089..2691)
                     /locus_tag="WNA25_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFA9823198.1"
                     /translation="MTDKISPDALIEAALLTQTEPLTEKSMRELCVPPLSQDKLIDVL
                     AQLKTRWQDRALQLVHTQEGWRFQIVQTAFERLGSLQEQRAPRYSRAVMETLAIIAYQ
                     QPVTRGDIEGIRGVAVSQNVMQTLQDRGWIEVIGHRDTLGKPALWATTATFLSDLRLD
                     GLEELPPLTELGELVLPDLIEMPPTDEEEPETVPSDTLPN"
     gene            complement(2792..3733)
                     /gene="ylqF"
                     /locus_tag="WNA25_000363"
     CDS             complement(2792..3733)
                     /gene="ylqF"
                     /locus_tag="WNA25_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFA9823199.1"
                     /translation="MAIQWFPGHMNKARKAIAERAKSVDMVIEMLDARMPASSENPLL
                     AQLSKGKPKLKILNKQDLADPERTKVWLDHYNSRPDTRAIALDSSETGAHGKITQACR
                     AMIPHRQGIDKPLRVLICGIPNVGKSTLINGMIGKKSAKTGNEPGITKAEQRLFLADD
                     FWLYDTPGMLWPKIIVEESGYNLAAGGAVGRNALDEEEVALELLDYLRRHYLPMLQER
                     YQADKDPSSHWDDNSWLEWIAKKRGAVLSGGRVNYQKAAENILTDFREGKIGRITLET
                     PNQWETWLKKARQKEAELKAIREARKAERKGQKPSEA"
     gene            complement(3814..4668)
                     /gene="rapZ"
                     /locus_tag="WNA25_000364"
     CDS             complement(3814..4668)
                     /gene="rapZ"
                     /locus_tag="WNA25_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFA9823200.1"
                     /translation="MKIVLISGLSGSGKSVALRQMEDLGYFCVDNLPLEMLPSLVSYH
                     IERADETELAVSVDVRSGIDIAQAREQIAYLRGLGHRVEVLFVEAEEAVLVRRFSETR
                     RGHPLSNQDMTLLESLKKEREWLFPLKEIAYCIDTSKMNAQQLRHAVRQWLKVERTGL
                     LVVLESFGFKYGVPNNADFMFDMRSLPNPYYDPELRPYTGMDKPVWDYLDGQPLAQEM
                     VDGIERFVTRWLPRLEDESRSYVTVAIGCTGGQHRSVYIVEKLARRLKGRYELLIRHR
                     QAQNLSGR"
     gene            complement(4649..5611)
                     /locus_tag="WNA25_000365"
     CDS             complement(4649..5611)
                     /locus_tag="WNA25_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HFA9823201.1"
                     /translation="MPSISVRRLFDDNQYKLQLAWAAGNSGADNRIGVEADKPVLALV
                     GHLNFIHPNQIQVVGLAESEYLNRLESGETGYQFGDLFDISMSLVIVANDLPVSPGLR
                     DYCHKNDIPLLTSKLESPYLMDVLRIYLQRTLAASSVKHGVFLDVFEIGVLITGHSGL
                     GKSELALELISRGHSLIADDAVELFRIGPETLEGRCSPMLRDFLEVRGLGILNIRHIF
                     GETSIRPKKILQLIINLVEADDEYMKQLDRLSIRTETESILNVNVRSVTLPVAVGRNL
                     AVLVEAAVRNYILQLRGKDSTREFLERHQTQLKENEQNHENRPD"
     gene            complement(5615..6064)
                     /gene="ptsN"
                     /locus_tag="WNA25_000366"
     CDS             complement(5615..6064)
                     /gene="ptsN"
                     /locus_tag="WNA25_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFA9823202.1"
                     /translation="MSLIGEILPLSHIVLDMEVGSKKRLFEEAGLLLERESSLSHADV
                     FECLFAREKLGSTGLGQGVAIPHGRHASVKQATGAFIRTREPVGFDAPDGKPVSLVFI
                     LLVPENATGEHLEVLSKLAGKFSQKSIRESLMTVASAEEARAILTEE"
     gene            complement(6249..7139)
                     /gene="ubiA"
                     /locus_tag="WNA25_000367"
     CDS             complement(6249..7139)
                     /gene="ubiA"
                     /locus_tag="WNA25_000367"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFA9823203.1"
                     /translation="MNLKSPLFLRLSDRLDVYIRLMRADKPIGTLLLLWPTYWALWLA
                     SDGIPDLAVLAAFTIGTFLMRSAGCVINDFADRDFDGAVERTKNRPFAQGRVKKKEAL
                     LLTAFLCLLAALCLIPLNHLTWLMSLPALFLALTYPFTKRFFPIPQFYLGLAFSFGIP
                     MAFAAVGNSVPVEAWILFAANVLWTLAYDTVYAMADKEDDLKIGIKTSAVTFGRYDIA
                     AVMLCHGGFTLLMAVLGAVIGAAWAYWTAIPIVLLLQYRQYAAIKSRVRQICFETFLA
                     NNRIGWVWFAAIFAHTFFAK"
     gene            complement(7129..7536)
                     /locus_tag="WNA25_000368"
     CDS             complement(7129..7536)
                     /locus_tag="WNA25_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823204.1"
                     /translation="MNIIEIISRNRFLKQIYPSGIMDISLVSFSTDLSNCILTIRTST
                     KPSVEIEKWGLWLKDYDTVEIELRNSFIKGMKCQNWSHNNRNICQVEIKNQEDGLKII
                     RFYDNNSNWLLELEVYGLVFQGCKTYMKEGYES"
     gene            complement(7545..7640)
                     /locus_tag="WNA25_000369"
     CDS             complement(7545..7640)
                     /locus_tag="WNA25_000369"
                     /inference="COORDINATES: protein motif:HMM:NF026993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823205.1"
                     /translation="MRQPASKPHFNIRDSNLDTGHVDGTHGHYNF"
     gene            complement(7660..8136)
                     /locus_tag="WNA25_000370"
     CDS             complement(7660..8136)
                     /locus_tag="WNA25_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFA9823206.1"
                     /translation="MEHLFGKWLPDLPAPVSDGIDLPMSRLLKARSLTAALCALPHTF
                     SVELLKLGEAETEYGRRRVRDVLLKLDGTAVVQARSACSVGSAFWQNILDCGTRPLGE
                     RLFQADLEGARSAFEFAVSSEGCGRYFAARRSRFSHQGEEMLLTEYFLPELKRFIG"
     gene            complement(8151..8798)
                     /gene="bioD"
                     /locus_tag="WNA25_000371"
     CDS             complement(8151..8798)
                     /gene="bioD"
                     /locus_tag="WNA25_000371"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HFA9823207.1"
                     /translation="MKGVYFVSGIDTDIGKTVATGMLAKQLLQQGKSVITQKPVQTGC
                     QDIAEDIAVHRKIMGIPMQEADEQRLTMPEIFSHPASPHLAARLDGRGLDLDKIRTAT
                     QELAAQYEVVLVEGAGGLMVPLTEKLLTIDHIQQQAYPVILVTSGRLGSINHTLLSFV
                     VLKQYGIRLHSLVFNHIHDSRDAHVAQDSLNYLQCRLKADFPEAEWMELAKTGAV"
     gene            complement(8795..10096)
                     /gene="bioA"
                     /locus_tag="WNA25_000372"
     CDS             complement(8795..10096)
                     /gene="bioA"
                     /locus_tag="WNA25_000372"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFA9823208.1"
                     /translation="MPSEHQHTSSLLNFDRTHLLHPYTSMTDPLPVYPVKRAEGVFIE
                     LADGTRLIDGMSSWWCAIHGYNHPVLNQAVENQMKQMAHVMFGGLTHEPAVELGKLLV
                     GILPQGLDRIFYADSGSVSVEVALKMAVQYQQARGLTAKQNIATVRRGYHGDTWNAMS
                     VCDPETGMHHIFGSALPQRYFVDNPKNRFDDEWDGADLQPVRALFEAHHVDIAAFILE
                     PVVQGAGGMYFYHPQYLRGLRDLCDEFDIVLIFDEIATGFGRTGKMFACEHAEVVPDI
                     MCIGKGLSGGYMTLAAAITSQKVTETISRGEAGVFMHGPTFMANPLACAVACASVKLL
                     LSQDWQANIRRIESILKGRLKAAWDIRGVKDVRVLGAIGVIELEKGVDMARFQADCVA
                     QGIWVRPFGRLVYLMPPYIISDGILTKLADKTVQILKEHSK"
     gene            complement(10730..11215)
                     /gene="fxsA"
                     /locus_tag="WNA25_000373"
     CDS             complement(10730..11215)
                     /gene="fxsA"
                     /locus_tag="WNA25_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HFA9823209.1"
                     /translation="MRFFGIGFLVLLFLEIMSIVWVADWLGGGWTLFLMAATFAAGVL
                     MLRHTGLSGLLLAGAAVKSSGKVSVYQMLWPIRYTVAAVCLMSPGFVSSVLAVLLLLP
                     FKGGAVLQAGGAENFFNMNQSGRKEGFFHDDDIIEGEYTVEEPYGGNRSRNAIEHKKD
                     E"
     gene            complement(11391..11699)
                     /locus_tag="WNA25_000374"
     CDS             complement(11391..11699)
                     /locus_tag="WNA25_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823210.1"
                     /translation="MTNNVDLTIPAKRYFTLDELCGLLQISPYGFAQWQHDHGVVVGY
                     GGERYTRLDVVKLLKLKSTFAPYAEGAESGSDGNRPVTLQEIGDGLKDLLEDLDKELC
                     "
     gene            complement(11683..11985)
                     /locus_tag="WNA25_000375"
     CDS             complement(11683..11985)
                     /locus_tag="WNA25_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFA9823211.1"
                     /translation="MTLTKAELADILVDKVSNVTKNDAKEIVELFFEEIRSTLASGEE
                     IKISGFGNFQLRDKPQRPGRNPKTGEEVPITARRVVTFHASQKLKGMVEHYYDKQR"
     gene            complement(12059..14422)
                     /locus_tag="WNA25_000376"
     CDS             complement(12059..14422)
                     /locus_tag="WNA25_000376"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFA9823212.1"
                     /translation="MQFSYSWLKTQANPDLSADKLEHLLTMAGLEVEEIDTAAPAFSG
                     VVVAEVKSVEKHPDADRLNVTQVDAGTGELVQIVCGAPNVKPGIKVPCSLPVAVLPGN
                     FKIKPTKMRGVPSNGMLCSTNELGLPDDGVDGLHILPEDAPVGTNIREYLDLDDMLFT
                     LKITPNRADCLSVKGIAREVSALTQCAFTPVEIQTAPIGSEKKQAVRIDAPADCGRFI
                     SRVIENVNAKAATPDWMKQRLERSGIRSISALVDIGNYVMLEIGQPMHVFDADKLSGS
                     LIVRRAQNGETLACLNEKTVTLADNTLVVADEKGALSLAGLMGGAASAVSDGTQNIVL
                     EAAWFEPEIIVGKSRQYGFGSDSSFRFERGVDYRLQADAIERATELVLQICGGAAGEM
                     VEAQGKLPEAKQVGLRLGRLKTVLGVDIPAEQVETILQHLGLQPEKTAEGFRITAPSF
                     RFDIEIEADLIEEIGRVYGYENIPDDYTSGRLKMSELPETRRPRFAVYNEMAARGYRE
                     VISYAFVDEQWELDFAANAAPIRLQNPLAAQYAVMRSTLIGGLVEILQNNLNRKQNRV
                     RVFEIACVFGKGSDGRFVQNERIGGLWYGAAMPEQWGEKTRNADFYDIKADVENLLKN
                     KAVEFVKTGHPALHPGRAANIVSDGKVIGFVGELHPKWLQKYDLPQAPLVFEIDMAAV
                     LECGKTRYRAVSKFQPVRRDLAFVMPEVMNHDDLLLVLKGAANKLVQEISVFDVYRGT
                     GLPEGMKSVAVKVILQDMENTLTDEAVEPLIGKLIGVATEAGARLRS"
     gene            complement(14513..15262)
                     /locus_tag="WNA25_000377"
     CDS             complement(14513..15262)
                     /locus_tag="WNA25_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HFA9823213.1"
                     /translation="MKKWTRDETLVALYLYYIIPFQKVSKDNPVIQEYAEILGRTPSA
                     LGMKIGNLGRLDPTLKVKNISGLSNGSKMDVVVWNEFSGDWEQLNKEFEGVISQYQSN
                     DENSNIEIESPEIPKGRERFARISVRVNQGFFRSSVLAAYNNQCCITGLKQPELLVAS
                     HIKPWGEDKDNRLNPRNGLCLNALHDKAFDRGLLGIDENFKIIFSPLLAKTEGFDDLF
                     KPYENRMIRLPERLNPSLEFLKFHRENIFQS"
     gene            complement(15268..15513)
                     /locus_tag="WNA25_000378"
     CDS             complement(15268..15513)
                     /locus_tag="WNA25_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823214.1"
                     /translation="MRLSDYILNNKININNLSDKQVQEIIDYFSSEIDDWLLASEKEL
                     KDYDVYLYKLVLDGQQYVQGIRKSVLYDFLMENQKDS"
     gene            complement(15527..15865)
                     /locus_tag="WNA25_000379"
     CDS             complement(15527..15865)
                     /locus_tag="WNA25_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFA9823215.1"
                     /translation="MIYKNKIKEDGQDERLKEKAAQYRLFENNDTFQTALKPKFTFID
                     LFAGIGGFRIAMQNLGGEYVFSSEWDEKAKLTYEANFGEVPFGDITLEEIKQYIPKQF
                     DVLCGFTLSK"
     gene            complement(15867..16280)
                     /locus_tag="WNA25_000380"
     CDS             complement(15867..16280)
                     /locus_tag="WNA25_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HFA9823216.1"
                     /translation="MDKLTPEQRKKCMQSNKSTGTKPELVLAKAMWALGLRYRKNSGS
                     IFGKPDFSFKKYKVAVFVDGEFWHGKDWEQKKAVIKGNREFWIAKIERNIQRDIEVTG
                     RLKAEGWTVLRFWSNDVVKNTTCCAEKVKEIIQTR"
     gene            complement(16297..17286)
                     /gene="pheS"
                     /locus_tag="WNA25_000381"
     CDS             complement(16297..17286)
                     /gene="pheS"
                     /locus_tag="WNA25_000381"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFA9823217.1"
                     /translation="MENVNRIVAEGIAAVEAAQDFNALEQIKARYLGKTGELTGLLKT
                     LGQMSPEERKTIGAHINECKNRFQTAFNAKRDALNEAKLQARLAAEALDITLPGRAQE
                     GGSLHPVTLTLQRVVELFHGMGFEVADGPEIEDDFHNFQALNIPANHPARAMQDTFYV
                     ENGDVLRTHTSPIQIRYMLDKKEPPIRIIAPGRVYRVDSDATHSPMFHQAEGLWVEEG
                     VTFADLKAVFTDFIRRFFERDDLQVRFRPSFFPFTEPSAEIDIMGENGKWLEVGGCGM
                     VHPNVLKNVNIDPEKYTGFAFGIGLDRFAMLRYNVNDLRLFFDNDLNFLKQFE"
     gene            complement(17527..17886)
                     /gene="rplT"
                     /locus_tag="WNA25_000382"
     CDS             complement(17527..17886)
                     /gene="rplT"
                     /locus_tag="WNA25_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFA9823218.1"
                     /translation="MPRVKRGVTARARHQKIFALAKGYRGRRKNVYRVAKQAVMKAGQ
                     YAYRDRRQRKRQFRQLWIVRINAGARENGLSYSKFMNGLKRASIEIDRKVLADLAVFD
                     KAAFAQLVEKAKAALAA"
     gene            complement(17899..18096)
                     /gene="rpmI"
                     /locus_tag="WNA25_000383"
     CDS             complement(17899..18096)
                     /gene="rpmI"
                     /locus_tag="WNA25_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFA9823219.1"
                     /translation="MPKMKTKSSAKKRFKVLGNGGVKRAHAFKRHILTKKTTKNKRQL
                     RGTSMVNDRDLASVAKMLPYA"
     gene            complement(18243..18764)
                     /gene="infC"
                     /locus_tag="WNA25_000384"
     CDS             complement(18243..18764)
                     /gene="infC"
                     /locus_tag="WNA25_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFA9823220.1"
                     /translation="MIAQEREARINGEITAKEVRLISESGEQLGVVSVREALAMAEGQ
                     DVDLVEISPTAKPPVCKLMDYGKYKYQQAKKRDEAKKNQKQVQIKEIKFRPGTDEGDY
                     QIKMRNINRFLADGDKVKVTLRFRGREMAHQQLGAQLLERVKEDLAEVAQIESFPKME
                     GRQMVMMIAPKKK"
     gene            complement(18782..20695)
                     /gene="thrS"
                     /locus_tag="WNA25_000385"
     CDS             complement(18782..20695)
                     /gene="thrS"
                     /locus_tag="WNA25_000385"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFA9823221.1"
                     /translation="MLNITLPDCSVRQYESPVTVAQIAASIGAGLAKAAVAGKVNGKL
                     VDACDPIVEDSAVQIITPKDQEGIEIIRHSCAHLVGHAVKQLYPNAKMVIGPVIEEGF
                     YYDIATEKPFTPEDVAAIEARMKELIAQDYDVVKIMTPRAEAIKIFQERGEEYKLRLI
                     EDMPEVEAMGMYHHQEYVDMCRGPHVPNIRFLKNFKLTKLAGAYWRGDSNNEMLQRIY
                     GTAWATKDELKDYIQRIEEAEKRDHRKLGKQLDLFHLQDEAPGMVFWHPKGWALWRTI
                     EQHMRKELNAAGYKEVKTPQIMDKTFWEKSGHWDNYKDNMFVTSSEKREYAVKPMNCP
                     GHVQIFNNGLRSYRDLPMRLAEFGSCHRNEPSGALHGLMRVRGFVQDDAHIFCTEDQI
                     VSEARAFNELLVRIYKQFGFHDVSVRLSLRPEKRAGSDDVWDKAEQGLREALTACGVE
                     WGELPGEGAFYGPKIEYHVKDALGRSWQCGTLQLDFVLPERLDAEYVTENNDRARPVM
                     LHRAILGSLERFVGILIENHAGSFPLWLAPVQMVIMNITENQADYCREVAAKLQAAGF
                     RAELDLRNEKIGYKIRDNSQYRFPYQIVIGDKEKQENKVAVRRKAEDLGSLDLDDFIA
                     QLQQEITDALVNH"
     gene            complement(20748..20824)
                     /locus_tag="WNA25_000386"
     tRNA            complement(20748..20824)
                     /locus_tag="WNA25_000386"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20788..20790),aa:Val,seq:gac)
     gene            21003..22118
                     /gene="tgt"
                     /locus_tag="WNA25_000387"
     CDS             21003..22118
                     /gene="tgt"
                     /locus_tag="WNA25_000387"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFA9823222.1"
                     /translation="MLKFTLHKKDGLARRGTLELNHGKIETPVFMPVGTYGSVKAMNP
                     QNLHDIKAQIILGNTYHLWLRPGLEVVEQFGGLHGFIGWDKPILTDSGGFQVFSLSDM
                     RKLTEEGCTFKSPINGDKLFLSPEISMKIQTVLNSDIAMQLDECTPGETTREQARKSL
                     QMSLRWAERSKKAFEDLKNPNALFGIVQGAMYEDLREESLRGLEEFDFPGLAVGGLSV
                     GEPKPEMYRMLHAVGPMLPEHKPHYLMGVGTPEDLVYGVAHGIDMFDCVMPTRNARNG
                     WLFTRFGDLKIKNAKHKPDKRPIDESCTCYACQNFSRAYLHHLHRAGEILGAQLNTIH
                     NLHFYQVIMAEMRDAVEQGKFADWQAQFHENRARGVD"
     gene            22128..23210
                     /locus_tag="WNA25_000388"
     CDS             22128..23210
                     /locus_tag="WNA25_000388"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFA9823223.1"
                     /translation="MPSEAASDSILPLYPTCTIFQKPIMLPFLPEPSLSYTQQNRTAV
                     LLLNLGTPDAPTAQAVRPYLKSFLTDRRIVELPKWLWYPILHGLVLTFRPKKSAHAYE
                     KIWFKEGSPLEVYTARQAAALAERMPDLIVRHAMTYGNPSIADVLAELKSQGVGRLLA
                     IPLYPQYTASSSGAAVDKVCEQLLLQRNQMSVRTISRFYDDAGYIDAMKNHILRYWAE
                     HGRGKKLMLSFHGVPQKHYDLGDPYPDECRHTAKLLAEALELTEDEYTVSFQSQFGRA
                     KWVTPSTQDLFGKLPKQGVTELDVFCPGFLADCLETMEEIALTGREQFYEAGGKNYRY
                     IPCLNDNPDWIDALVALAEENLGGWR"
     gene            23401..23868
                     /locus_tag="WNA25_000389"
     CDS             23401..23868
                     /locus_tag="WNA25_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c2"
                     /protein_id="HFA9823224.1"
                     /translation="MNTTRLPTAFILCCLCAAASAADNSIMTKGQKVYESNCIACHGK
                     KGEGRGTMFPPLYRSDFIMKKPQVLLHSMVKGINGTIKVNGKTYNGFMPATAISDADI
                     AAVATYIMNAFDNGGGSVTEKDVKQAKGKKNQTDKMPSETGNPASDGIQTKPF"
     gene            complement(23865..25595)
                     /locus_tag="WNA25_000390"
     CDS             complement(23865..25595)
                     /locus_tag="WNA25_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFA9823225.1"
                     /translation="MDWMNSLFLLGGALLFLSVVSTTLSARLGMPLLLVFLAVGMLAG
                     EEGVGGIAFNNVVMANFISQLALAVILLDGGLRTQLSSFRIALKPASVLASWGVFATV
                     LPLGLFATFYLGLDWKFGVLMAAIVGSTDAGAVFSLLRNSGVRLNERVQATLEIESGA
                     NDPMAVFLVTALITMIMQPAESGAAAFVRMLALQIGFGLLTGWAGGKILAKLVRRLNL
                     AEGLYALMIVSGGLLVFAFTNTIGGSGFLAVYLAGIIVGNQRNRATEHVLRVMDGLAW
                     LAQATLFVMLGLLVSPAGVLDRAAEALAIAAFLMLVARPLAVFGGLWKFNYSLREKAY
                     ISWLGLRGAVPISLAMMPLVMGVPNSHLLFDVAFAVVVLSLLIQGTTIPVMARLLKVA
                     MPNKPEPKDTHDIWLAEKEIVRMSAFKVVAESEAEGHHPDTVEPISDSFDARCFALIR
                     NGSRIEMQSDTVLQAEDLAWYILPDGKVDKMAKYFTETGIGVRENFDFFGEFVVSPAA
                     RSGDLALAYGLKLEAGEEGLSLAELFDKRSDSQEPVEGDRIDIGGFMLTAKEVDGDGN
                     IGSMGLKVPR"
     gene            25790..26530
                     /locus_tag="WNA25_000391"
                     /pseudo
     CDS             25790..26530
                     /locus_tag="WNA25_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688345.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
     gene            26599..28137
                     /gene="lnt"
                     /locus_tag="WNA25_000392"
     CDS             26599..28137
                     /gene="lnt"
                     /locus_tag="WNA25_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFA9823226.1"
                     /translation="MFSKLDKYWQHPALYWPLLILFAAATPFAFAPYYHFWLMPLIFG
                     AFVRLIELRPRFAASSAYLFGLTAYTAQFYWIHTALHDVSGLPDLYAVPLTFLLPAYL
                     ALYPALCFWLWKKFTLPQGIKTGLVLPILWTLTEFARERFLTGFGWGAIGYSQITPDS
                     PLAGFAPLGGIHMVTLATAFLGVWLVLAIDNTARSGKRLLPAILIAALLAAGYTAQQT
                     DFTRPDGSRSTVALLQGNIAQTLKWREDQVIPTIQKYYEQVGKTTADIVILPETAIPI
                     MRQNLPENILAQFAEQAQNNGSALAVGISQYTSDGNGYENAVINLTGYQENNQDGIPY
                     YAKNHLVPFGEYKPLPLLTTPLYKMMDMPLSDFRKGGGKQSAMMMKNQKIAFNICYED
                     GFGDELIAAAKDATLLANVSNMAWYGKSNAMYQHLQQSQARAMELGRYMVRATNTGAT
                     AIISPKGNIIAQAQPDTETVLEGHIKGYIGETPYMKTGSSWWLMGILTLAALILFIFR
                     NKEH"
     gene            28401..29264
                     /gene="rpoH"
                     /locus_tag="WNA25_000393"
     CDS             28401..29264
                     /gene="rpoH"
                     /locus_tag="WNA25_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFA9823227.1"
                     /translation="MNNAFALPAIQSGNGSLEQYIHTVNSIPMLSQEEETRLAERRIK
                     GDLNAAKQLILSHLRVVVSIARGYDGYGLNQADLIQEGNIGLMKAVKRYEPGRGARLF
                     SFAVHWIKAEIHEFILRNWRLVRVATTKPQRKLFFNLRSMRKNLNVLSPKEAQDIADD
                     LGVKLSEVLEMEQRMTGHDIAIMADNSDDEDSFAPIDWLADHDSEPSRQLSKQAHYAL
                     QTEGLQNALAQLDDRSRRIVESRWLQDDGGLTLHQLAAEYGVSAERIRQIEAKAMQKL
                     RGFLTEEAEAV"
     gene            complement(29421..29897)
                     /locus_tag="WNA25_000394"
     CDS             complement(29421..29897)
                     /locus_tag="WNA25_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFA9823228.1"
                     /translation="MESRAFVAARKGNVSSKISKTRTGYGFFAKMIFASRWLQVPIYA
                     GLTVVRAICAYKFLKSLKYLVMNLDVSDENAIMLAVLNLIDVVMIANLLTMVQIGGYE
                     SFVSRLRIDDHPDRPEWLSHVNAPVLKVRLSMSIIGIHPSIYSKHLSIPPICRKSS"
     gene            29997..30881
                     /locus_tag="WNA25_000395"
     CDS             29997..30881
                     /locus_tag="WNA25_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HFA9823229.1"
                     /translation="MSSGNIRIHSMTGFANAAAECGSKRINLDIRAVNHRFLDIQIRM
                     PDDLRHLENSIREQISSHLARGKVECKIQIQDAENGNQSLELNRVLVGQLAEINKNLR
                     KHYDLAKLGVADILRFPGVLASQKENTEALAKTIVELADKALKDFTAARRREGKKLGE
                     HLLQRIKNMEEIIDALSEIFPALVEAHKEKIRTRLAEAVGSIDNDRLQQEFALFIQKS
                     DIDEEFSRLRTHIAEVRRIVTEHKGSSGKRLDFLMQELNREANTLGSKSIATECTQAS
                     VELKVLIEQMREQVQNIE"
     gene            complement(30972..31487)
                     /locus_tag="WNA25_000396"
     CDS             complement(30972..31487)
                     /locus_tag="WNA25_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFA9823230.1"
                     /translation="MMILKTAPLKRRFAAILYEMLLVGAATCLAALLAGIAAIFLNPV
                     SIAVSALVTSILIMGAWWLYFRANWHGQGQTLAMRTWKIGLCDLNGIQPSLHLLRLRF
                     IWACIFIVFIPMLAYAGLRHFLGIPPKGAAGAALVWLILPWGFALLNPDRQFLYDFLA
                     GTRLVDVKEQS"
     gene            complement(31563..31982)
                     /locus_tag="WNA25_000397"
     CDS             complement(31563..31982)
                     /locus_tag="WNA25_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823231.1"
                     /translation="MDNKTKLRLGGLILLTTAVLSLIIVLIVDSWPLAILLAAVIVAA
                     AAGGFVWTSRRQQRQFIERLKKFDIDPEKGRINEANLRRMYHSGGQHQKDAITLICLS
                     QKCSVDEAHAMFKKRPTRQEINQMAAKQSRGQKRPHR"
     gene            complement(32103..33101)
                     /locus_tag="WNA25_000398"
     CDS             complement(32103..33101)
                     /locus_tag="WNA25_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFA9823232.1"
                     /translation="MAAHIGRIDTDAPLKPLYVIHGEEELLRIEAVDALRAAAKKQGY
                     LNREAYTADASFDWNELLQTAGSAGLFADLKLLELHIPNGKPGKNGGEALQDFAARLP
                     EDTVTLVLLPKLEKTRLQSKWFAALAAKGEVWEAKPVGAAALPQWIRGRLDKIGLGIE
                     ADALALFAERVEGNLLAARQEIDKLALLYPKGHAVNIDEAQTAVANVARFDAFQLAGA
                     WMKGDVPRVCRLLDGLEEEGEEPVLLLWAVAEDVRTLIRLAAALKQGQSIQSVRNSLR
                     LWGDKQTLAPLAVKRISVVRLLDALKTCAQIDRIIKGAEDGDAWTVFKQLVVSLAE"
     gene            complement(33103..33582)
                     /locus_tag="WNA25_000399"
     CDS             complement(33103..33582)
                     /locus_tag="WNA25_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823233.1"
                     /translation="MNKIFLTAAALVLGACGFHLKGADGISPPLTYRSWHIEGGQALQ
                     FPLETALYQASGRVDDAAGAQMTLRIDSVSQNKETYTVTRAAVINEYLLILTVEAQVL
                     KRGEPVGKPMTVSVRRILDYADNEILGKQEEEETLWAEMRQDVAEQIVRRLTFLKAE"
     gene            complement(33634..34413)
                     /gene="pgeF"
                     /locus_tag="WNA25_000400"
     CDS             complement(33634..34413)
                     /gene="pgeF"
                     /locus_tag="WNA25_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HFA9823234.1"
                     /translation="MKTITETLNLAPKGKNFLTADWPAPANVKTLITTRNGGVSQGAY
                     QSLNLGTHVGDDPETVRRNREIVQQQVGLPVAYLNQIHSTIVVNAAEALDGTPDADAS
                     VDDTGKAVCAVMTADCLPVLFCDRAGTAVAAAHAGWSGLAGGVLQNTIAAMKVPPVEI
                     MAYLGPAISADAFEVGQDVFDAFCTSMPEAAAAFEPIGGGKFLADLYALARLILRREG
                     VGGVYGGTHCTVLERDIFFSYRRDGATGRMASLIWLDGNAV"
     gene            34598..34789
                     /locus_tag="WNA25_000401"
     CDS             34598..34789
                     /locus_tag="WNA25_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823235.1"
                     /translation="MLQQGTAHQAPHCVLPERGCPPIRFYRYKQTGFNRKGMGIKSIS
                     DTSRAMPSENQSPLSDGIV"
     gene            35007..35813
                     /locus_tag="WNA25_000402"
     CDS             35007..35813
                     /locus_tag="WNA25_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFA9823236.1"
                     /translation="MFGMGLTLKPSDFDILFKHPKAVIIGVIAQFAIMPATAWLPSKL
                     LNLPAEIAVGVILVGCCPGGTASNVMTYLARGNVALSVAVTSVSTLISPLLTPAIFLM
                     LAGEMLEIQAAGMLMSIVKMVLLPIVLGLIVHKVLGSKTEKPTDALPLVSVAAIVLII
                     GAVVGASKGKIMESGLLIFAVVVLRNGIGHLLGFFAAKWTGLPYDAQKTLAIEVGMQN
                     SGLAAALAAAHFAAAPVVAVPGALFSVWHNISGSLLATYWAAKAGKHKKP"
     gene            complement(35966..37090)
                     /gene="rluD"
                     /locus_tag="WNA25_000403"
     CDS             complement(35966..37090)
                     /gene="rluD"
                     /locus_tag="WNA25_000403"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFA9823237.1"
                     /translation="MQNTSFDNESDYSDDSDFASASGTENRIGLTVPLELAGGRLDAV
                     LAKLLPDYSRSRLTSWIKEGAVIVNDKPSQPKDKMIGGEQICVTVRPSEENLAFVPES
                     MDLDIVYEDDTVIVVNKPTGLVVHPAAGNWTGTLLNGLLAHCPELSQIPRAGIVHRLD
                     KETSGLMVVAKTLPAQNSLVRQLQERTVKRIYRAVANGIVPFDGKIETQIGRDPHNRL
                     KMAAVKFGGKPAVTHVKVLERYLAHSYIECSLGTGRTHQIRVHMREANHPLAGDPVYG
                     NPRHPCGDTVKEAVKSLGARQALHAYRLSFTHPESGETVSFEAPIPDDIYHLLSVLRL
                     EAGLDSSLSNEEEWQDKFGADDDDDWNEDDYDVEVVYVRE"
     gene            37089..37892
                     /locus_tag="WNA25_000404"
     CDS             37089..37892
                     /locus_tag="WNA25_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFA9823238.1"
                     /translation="MKKILLTVSLGLALSACATQGTADKDAQITQDWSVEKLYAEAQD
                     ELNSSNYTRAVKLYEILESRFPTSRHARQSQLDTAYAYYKDDEKDKALAAIERFRRLH
                     PQHPNMDYALYLRGLVLFNEDQSFLNKLASQDWSDRDPKANREAYQAFAELVQRFPNS
                     KYAADATARMVKLVDALGGNEMSVARYYMKRGAYIAAANRAKKIIGSYQNTRYVEESL
                     AILELAYKKLDKPQLAADTRRVLETNFPKSPFLTHAWQPDDMPWWRYWH"
     gene            complement(37962..40181)
                     /locus_tag="WNA25_000405"
     CDS             complement(37962..40181)
                     /locus_tag="WNA25_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFA9823239.1"
                     /translation="MFRRWFLPCWVVGVAVSFALSVVPHWPFWLAAFAVLAVLARRFA
                     FAGLMLCVLAGAAYGVFRTEAALSSQWRAEAVSGVPLTVEVTDMPRSDGRRVQFAAKA
                     VDSGGRTFDLLLSDYKRREWAVGSRWRITARVHPVVGELNLRGLNREAWALSNGVGGV
                     GTVGADRVLLHGGSGWGIAVWRSRISRNWRQADADGGLSDGIGLMRALSVGEQSALRP
                     GLWQAFRPLGLTHLVSISGLHVTMVAVLFAWLAKRLLACSPRLPARPRAWVLAAGCAG
                     ALFYALLAGFSVPTQRSVLMLAAFAWAWRRGRLSAWATWWQALAAVLLFDPLAVLGVG
                     TWLSFGLVAALIWACAGRLYEGKRQTAVRGQWAASVLSLVLLGYLFASLPLVSPLVNA
                     VSIPWFSWVLTPLALLGSVVPFAPLQQAGAFLAEYTLRFLVWLADVSPEFAVAAAPLP
                     LLVLAVCAALLLLLPRGLGLRPWAVLLLAGFVSYRPEAVPENEAAVTVWDAGQGLSVL
                     VRTANRHLLFDTGTVAAAQTGIVPSLNAAGVRRLDKLVLSHHDSDHDGGFQAVGKIPN
                     GGIYAGQPEFYEGARHCAEQRWQWDGVDFEFLRPSERKNIDDNGKSCVLRVVAGGAAL
                     LVTGDLDTKGEESLVGKYGGNLYSQVLVLGHHGSNTSSSGVFLNAVSPEYAVASSGYA
                     NAYKHPTEAVQNRVRAHGIKLLRTDLSGALQFGLGRGGVKAQRLRVYKFYWQKKPFE"
     gene            40213..40488
                     /locus_tag="WNA25_000406"
     CDS             40213..40488
                     /locus_tag="WNA25_000406"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823240.1"
                     /translation="MIKQSHKTVITRFENDKNANEKPQYYPRQRQTFHRHSRKSGNPK
                     TKSRRNLSERTEPQKPASHLRLNNRTKKAEIKTSGFFFKLAIKHQNE"
     gene            40567..>44110
                     /locus_tag="WNA25_000407"
     CDS             40567..>44110
                     /locus_tag="WNA25_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFA9823241.1"
                     /translation="MKAKRFKINAISLSIFLAYALTPYSEAALVRDDVDYQIFRDFAE
                     NKGKFFVGATDLSVKNKQGQNIGNALSNVPMIDFSVADVNKRIATVVDPQYAVSVKHA
                     KAEVHTFYYGQYNGHNDVADKENEYRVVEQNNYEPHKAWSASNLGRLEDYNMARFNKF
                     VTEVAPIAPTDAGGGLDTYKDKNRFSSFVRIGAGRQLVYEKGVYHQEGNEKGYDLRDL
                     SQAYRYAIAGTPYKDINIDQTMNTEGLIGFGNHNKQYSAEELKQALSQDALTNYGVLG
                     DSGSPLFAFDKQKNQWVFLGTYDYWAGYGKKSWQEWNIYKKEFADKIKQHDNAGTVKG
                     NGEHHWKTTGTNSHIGSTAVRLANNEGDANNGQNVTFEDNGTLVLDQNINQGAGGLFF
                     KGDYTVKGANNDITWLGAGIDVADGKKVVWQVKNPNGDRLAKIGKGTLEINGTGVNQG
                     QLKVGDGTVILNQKADADKKVQAFSQVGIVSGRGTLVLNSSNQINPDNLYFGFRGGRL
                     DANGNDLTFEHIRNVDEGARIVNHNTDHASTITLTGKSLITNPNSLSVHSIQNDYDED
                     DYSYYYRPRRPIPQGKDLYYKNYRYYALKSGGNVNAPMPENGVAENNDWVFMGYTQEE
                     AKKNAMNHKNNQRISGFSGFFGEENEKGHNGALNLNFNGKSAQNRFLLTGGANLNGKI
                     SVTQGNVLLSGRPTPHARDFVNKSSARKDAHFSKNNEVVFEDDWINRTFKAAEIAVNQ
                     SASFSSGRNVSDITANITATDNAKVNLGYKNGDEVCVRSDYTGYVTCNTGNLSDKALN
                     SFDATRINGNVNLSQNAALVLGKAALWGQIQGQGNSRVSLNQHSKWHLTGDSQVHNLS
                     LADSHIHLNNASDAQSANKYHTIKINHLSGNGHFHYLTDLAKNLGDKVLVKESASGHY
                     QLHVQNKTGEPNQEGLDLFDASSVQDRSRLFVSLANHYVDLGALRYTIKTENGITRLY
                     NPYAGNRRPVKPAPSPAANTASQAQKATQTDGAQIAKPQDIVVAPPSPQANQAEEAKR
                     QQAKAEQVKRQQAEAGRKSAELSAKQRAGEEERRQTAQSQPQRRKRRAAPQDYMAVSQ
                     DRPKRRGHRSVQQNNVEIAQAQAELARRQQEERKAAELLAKQRAEAEREAQALAARRK
                     AEAEEAKHQAAELAHRQEAKRKAAESAKRKAEEEE"
BASE COUNT        11103 a        12336 c        10393 g        10278 t
ORIGIN      
        1 atgtcctaac cgctagacga tgggcgcata ttgtattttc ttactggcgc acccggagcg
       61 attcgaacgc ccgaccctct ggttcgtagc cagatactct atccaactga gctacgggtg
      121 cggcacaatc acttgttcaa gtgattaaga aagcgtgata atacggattt atctgagtcc
      181 tgtctattat tttagacttc tattttcagt tatttttgta actgattgat tttaaaataa
      241 actatttttc gacgcaagtt gcagcaattc tgccgcatgt tgccgcgtcg tatcggtaat
      301 aacttctccg cccaacatac gggcaacctc ttcgatccgt tgtatttcat ccaatatact
      361 gatttcgctg acggtttgct ctccctcgct gtgcttgcgc acccgccagt ggttttctcc
      421 gcaggatgcg acttgcggaa gatgggtaac ggcaagcacc tgatgttttc tgcccaacgc
      481 acgtaatgcc ttgccgacca tttcagccac tccccctcca ataccggtat cgacctcatc
      541 aaaaatcagg gtgggaactt gggtatattg gctggcaaca acctgtaagg caaggctgat
      601 acgcgccaat tcgccgccgg aggcgacttt gttcagcggg cggggcggat tgcctttgtt
      661 agcggcaact tgaaattgaa cctgctccaa accgtgtgcc gtcggcgacg aaggcaacag
      721 gacgatgtcg aaacgcgcgc ctttcatggc aaggtgttgc atatgctcgg tcgtttcgct
      781 actcaaacgc cctgccgcct gatggcgcat ggcagaaagg atgtgggcag cttcctgata
      841 ttcggcaaaa ttgtgggcaa cattatgctc gagcgcgtcc aaatcagcgg cagcttgaag
      901 actttgcagg cgttcttcga tttctgccaa cttggcaggc aactcttcgg gttcaatccg
      961 gtatttccgc gccatcccca tcagttcgcc catacgctgt tcctgtgccg ccaattcatt
     1021 ggggttgatg tcactgtgtc ctgccacatc gcgcatattg gcactgattt cgcccaattc
     1081 ggcctcgatg cttgccaaca tattcaggct ctcggcaaag cgcggctcga tgttttgcag
     1141 attggccaat agtttttgac attgatagat atggcgttgg atgccgttgt cgccgtcaat
     1201 cttgcttccg acttcttcgg cggcctgcaa caattcggca gaatgggcaa ggctgtcgtg
     1261 gctttggctg agagcttccc attcgccttg tttaatgtcc aactgattca gttcgttaaa
     1321 ctgccattcc agacgctccc gctcgataat gacggcatcg gcgtgttcct gcgcctcttg
     1381 gagggctttt ttcgcattgg cccagttttg ataaagctgc ctgacggttt ccgcctgcac
     1441 cctgccgccc acaaatgcgt ccaacaattc gcgctgggca gcttcctgat taagcgaatg
     1501 atggtcgttt tgcccgtgga tgtcgataag ctgcccgccg acggctttga gttgcgccaa
     1561 ggtagccgcc tgattgttga taaaactgcg gcttttgcct ttggcatcga taatgcggcg
     1621 gatactgagt tcttccccgc cctcatccaa cagcccttgt tcacgcaatt ctgcttttaa
     1681 agcaggcaaa tgggaaatat cgaacaatgc cgacaactgc gcttcttttg cgccgctgcg
     1741 gacttggctg taatcggcct tatcgcccaa cagcagacca atcgcatcca aagtaatgga
     1801 cttgcccgcg ccggtctcgc cggtcaaaac ggtaaaaccg ctttgaaaat ccagattcag
     1861 attttcgaca atgacaaaat cacgcaaaga aagtgttaga agcattgtga ggattcctta
     1921 gcaattctac cgacaaaagc aatatcaact ttagatatac gaattatatc taagattttt
     1981 taaattaaaa ataagttttt ccttaatttt gcttagtgtt tgtttttatc tgcttatttg
     2041 ttgcaatgcc gtctgaagca atgtgcgttt cagacggcat ttggaatttc agttgggcag
     2101 ggtatcggac ggtacggttt ccggctcttc ttcatccgta ggcggcattt ctatcaaatc
     2161 gggcaaaacc agttcgccca gttcggtcag cggcggcagt tcttccaaac cgtccaagcg
     2221 caaatcgctg agaaaagttg ccgttgttgc ccacaatgcg ggttttccca atgtgtcccg
     2281 atgtccgatg acttcaatcc acccccgatc ctgcaaggtc tgcatcacgt tctgcgacac
     2341 cgccacgccg cgtatacctt cgatgtcgcc gcgcgttacg ggctgctggt aggcgataat
     2401 cgccagtgtt tccatcacgg cgcgggagta gcgcggcgca cgctgttctt gcaggctgcc
     2461 cagccgctcg aatgccgtct gaacaatctg aaaacgccag ccctcctgcg tatgcaccag
     2521 ttgcaacgcc ctatcctgcc aacgcgtttt caactgcgcc aacacatcaa tcagtttgtc
     2581 ttgcgacaac ggcggcacac acagttcgcg catagatttt tcggtcagcg gttcggtttg
     2641 ggtcagcagt gcggcttcaa tcagcgcgtc gggagaaatt ttgtcggtca tacgggtatc
     2701 cgtgctgaaa cggcatgggt ttggatatgc cgtctgaaat cggttggagt agagagaagc
     2761 tgcctgaaaa atatttttca gacggcattc tttatgcttc cgaaggcttc tgccctttgc
     2821 gctctgcttt tctggcttcg cgaatggctt tgagttcggc ttctttctga cgtgcttttt
     2881 taagccaagt ttcccattgg ttcggtgttt caagggtgat tctgccgatt ttgccttcgc
     2941 ggaagtccgt gaggatgttt tcggcggctt tttggtagtt gacccgtccg ccgctgagga
     3001 ctgcgccgcg ttttttggct atccattcga gccacgagtt gtcgtcccag tggctgctgg
     3061 ggtctttgtc ggcttggtag cgttcttgca gcatagggag gtagtggcgg cggaggtagt
     3121 ctaaaagttc gagggcgact tcttcttcgt ccaatgcgtt gcgtccgact gcgccgccgg
     3181 cggcgaggtt gtagccgctt tcttcgacga tgattttcgg ccacagcatt cccggggtgt
     3241 cgtacagcca gaagtcgtcg gcaaggaaaa ggcgttgttc ggctttggta atgccgggtt
     3301 cgttgccggt tttggcggat tttttgccta tcattccatt gatgagggtg gatttgccga
     3361 cgttgggaat gccgcaaatc aaaacgcgca ggggtttgtc tatgccttgc cggtggggaa
     3421 tcatcgcgcg gcaggcttgg gtgattttgc cgtgtgcgcc ggtttcggag gaatcgaggg
     3481 cgatggcccg ggtgtcgggg cggctgttgt agtggtcaag ccagaccttt gtgcgttcag
     3541 ggtcggcaag gtcttgcttg tttaaaattt tgagtttggg cttacctttg gaaagctgcg
     3601 ccaacagtgg attttcgctg gaggcgggca tacgcgcgtc cagcatttca atcaccatat
     3661 caacgctttt ggcacgctcg gcgatagctt ttcttgcctt gttcatatgg ccgggaaacc
     3721 attggattgc catgtctgtt ctttcttctc aatatttgaa atgccgtctg aaacggcgga
     3781 cggggtttca gacggcataa tggtttgacg ggattagcgg cccgacaggt tttgcgcctg
     3841 cctgtgccgt atcagcaatt cataacgccc tttcaacctt cgggcgagtt tttcgacaat
     3901 atagaccgaa cgatgctgcc cgccggtaca gccgatggcg acggtaacgt agctcctgct
     3961 ttcatcctcc aaacgcggca accaacgcgt aacaaacctt tcgatgccgt caaccatttc
     4021 ctgcgccaac ggctgtccgt ccaaataatc ccaaacgggc ttgtccatac cggtgtaagg
     4081 cctcaactcg ggatcgtaat acgggttggg cagactgcgc atatcgaaca taaaatcggc
     4141 gttgttcggc acaccgtatt tgaacccgaa ggactccaaa accaccagca gtccggtacg
     4201 ctcaaccttc agccactgcc ggactgcatg gcggagctgt tgggcattca tcttggaagt
     4261 gtcgatacag taggcgattt ccttaagcgg gaacagccat tcgcgctctt ttttcaggct
     4321 ttccaacagc gtcatatcct gattgctcaa aggatgtccg cgccgggttt cggaaaagcg
     4381 gcggaccagc acggcctctt ccgcctcgac aaacaaaact tccaccctgt gtccgagtcc
     4441 gcgcagatag gcaatctgct cgcgcgcttg ggcaatgtct atgccggaac gcacatcgac
     4501 gctgaccgcc aattcggttt cgtccgcacg ttcgatatgg tacgacacca gcgacggcag
     4561 catttccaag ggcaggttgt ccacgcaaaa atagcccaaa tcttccattt ggcgcagtgc
     4621 aacggatttg cccgagccgg aaagcccgct aatcaggacg attttcatga ttctgttcgt
     4681 tttctttaag ttgcgtctga tggcgttcca aaaattcgcg cgtactgtct ttgccgcgca
     4741 actgcaaaat gtaattgcgt accgccgcct caaccaaaac ggcaaggttg cgtccgacgg
     4801 cgacgggcag cgtaaccgaa cggacgttga cgttgaggat ggattcggtt tcggtgcgga
     4861 tgctcaaccg gtcgagctgc ttcatatact cgtcgtccgc ctcgaccaaa ttgataatga
     4921 gttgcaggat ttttttgggg cggatggaag tttcgccgaa aatatggcgg atattgagta
     4981 tccccaagcc gcgcacttcc aaaaaatcgc gcagcatagg cgaacaacgc ccttccaatg
     5041 tttccggacc gatgcggaac agctcgaccg catcgtcggc aatcaggcta tggccgcgcg
     5101 aaatcagttc caatgccaat tcgctcttac ccaaaccaga atgtccggta atcagcacgc
     5161 cgatttcaaa cacatcgaga aatacgccgt gtttgacgga cgatgctgcc aaggtgcgtt
     5221 gcaggtaaat ccgcaacaca tccatcagat aagggctttc gagtttggaa gtcaacaggg
     5281 ggatgtcgtt tttgtggcag tagtcgcgca gccccgggga aaccggcaaa tcgtttgcca
     5341 caatgaccaa agacatggaa atatcgaaca ggtctccgaa ctgataaccc gtctcccccg
     5401 attcgaggcg gttcagatat tccgactccg ccaaaccgac cacttggatt tggttgggat
     5461 ggatgaaatt caggtgtccg accagggcga ggacgggctt gtccgcctct acgccgatac
     5521 ggttgtccgc acccgaattg ccggcggccc aggcaagttg caacttgtat tggttgtcgt
     5581 caaacaggcg gcggacggag atactgggca tattttattc ttcggtcagg atggcgcggg
     5641 cttcttccgc agaagcaacc gtcatcagcg attctctgat gcttttttgg gaaaacttgc
     5701 cggccagttt ggataagact tccaaatgct cgcccgttgc gttttccggc accagcaaga
     5761 taaaaaccag tgaaaccggt ttgccgtcag gcgcgtcgaa tccgacgggt tcgcgcgtac
     5821 ggatgaacgc gcccgtcgcc tgcttcacgc tcgcgtggcg gccgtggggg atggcgacac
     5881 cctgccccaa accggtcgaa ccgagttttt cacgggcaaa aagacattcg aaaacatcag
     5941 catgggacaa tgaggattcg cgttccaaaa gcaggcccgc ttcctcaaac agcctttttt
     6001 tactgcccac ctccatatcc aaaacaatat gggacaaagg caaaatttcg ccgataaggc
     6061 tcataagctt ctcttttcag acatcgcaaa acagaaggat tgtaccgact gccggggcaa
     6121 acctcaatcc cgcatacggt acgggctgac ataacacagc gtttcaaaaa catattttaa
     6181 cgcttttcgg cacagataga aatgccgtcc aaagcagttt acggttcttc agacggcatt
     6241 gccctgcctt atttcgcgaa aaacgtatgg gcaaaaatag cggcaaacca cacccaaccg
     6301 attctgttgt ttgccaaaaa cgtttcaaaa cagatttgcc ggacgcggct tttgatggcg
     6361 gcatattggc ggtattgcag cagcaggacg atggggattg ccgtccaata tgcccatgcc
     6421 gcaccgataa ccgcacccaa tactgccatc agcagggtaa agccgccatg acacagcata
     6481 acggcggcga tgtcgtaacg cccgaacgtg acggcggagg ttttgatgcc gattttcaaa
     6541 tcgtcctctt tgtccgccat tgcatagacg gtatcgtaag ccagtgtcca taaaacattg
     6601 gcggcaaaga gtatccacgc ttcaacggga acgctgttgc ccactgcggc aaacgccatc
     6661 gggatgccga aggaaaaggc aagtcccaga taaaattggg gaatcggaaa aaagcgtttg
     6721 gtaaacgggt aagtcagcgc aaggaacagc gcgggcaggc tcatcagcca tgtcagatga
     6781 ttcagcggaa tcaggcacaa tgcggcaagc agacacaaaa atgccgtcag cagcagggct
     6841 tcttttttct tgaccctgcc ctgtgcgaac ggacggtttt tggtgcgctc gacagcaccg
     6901 tcaaaatcgc ggtcggcaaa gtcgttgatg acgcagccgg cactgcgcat taaaaacgtg
     6961 ccgattgtaa acgccgccaa taccgccaaa tcgggaatgc cgtctgaagc cagccacaat
     7021 gcccagtagg tcggccacag taaaagcagc gtcccaatgg gcttgtccgc cctcatcagt
     7081 ctgatataaa cgtccaaacg atcggacagt cgtaaaaata aaggggattt aagattcata
     7141 accctctttc atataagtct tacacccttg gaaaactaat ccataaactt ctagttccaa
     7201 taaccaattt gaattattgt cgtaaaatct tattattttt agaccatctt cttggttctt
     7261 tatttctact tggcatatat ttctattgtt atgcgaccaa ttttgacatt tcattccttt
     7321 aataaagcta tttcttaatt caatttcaac tgtatcataa tcttttagcc acagccccca
     7381 tttttcgatt tctacagaag gctttgtact tgttcggata gttaaaatac aattagacaa
     7441 gtcagttgaa aaagagacta gtgaaatatc cattatgcca ctaggatata tttgttttag
     7501 aaaacgattc ctacttatta tttctattat attcatattt cttactaaaa attatagtgt
     7561 ccgtgagtac catcgacatg tcctgtatct aaattggagt ctcttatatt aaaatgtggt
     7621 ttagatgcag gttgccgcac agcttgaaaa aacggtattt tatccgataa agcgtttcag
     7681 ttcgggcaga aaatactcgg tcagcagcat ttcctcgcct tgatgggaaa accgagaacg
     7741 ccgcgcggca aagtaccgtc cgcatccttc actggaaacg gcaaactcaa acgccgaacg
     7801 cgccccttcc aaatcggctt gaaacagacg ctcgcccaaa gggcgtgtgc cgcagtccaa
     7861 aatgttttgc caaaacgccg aaccgacgct gcacgcgctt cgtgcctgca caacagcagt
     7921 gccgtccagt ttcagaagca catcgcgcac cctccgccgt ccgtattccg tttccgcttc
     7981 gcccagtttc agcagttcca ccgaaaatgt atgcggcaag gcgcacaatg cggcggtcag
     8041 cgaccgggct ttcaataacc ggctcatcgg caggtcgatg ccgtctgaaa cgggggcggg
     8101 caagtcgggc agccatttcc caaataggtg ttccatatct ttcccaatct ttataccgcg
     8161 cctgtttttg ccaactccat ccattccgct tcgggaaaat cggctttcag acggcattgc
     8221 agatagttca ggctgtcttg ggcgacgtgt gcgtcgcggc tgtcgtggat gtggttaaac
     8281 acgaggctgt gcaggcgaat gccgtattgt ttgagcacga cgaaactgag cagggtgtga
     8341 ttaatgcttc ccagccgtcc gctggtaaca aggatgacgg gataagcctg ttgctgaata
     8401 tgatcaatgg ttaacagctt ttccgtcagt ggtaccatca acccgcccgc gccttcgacc
     8461 aaaacgactt cgtactgcgc cgccaattct tgtgtggcgg tgcggatttt gtccaagtcc
     8521 aaacccctgc cgtccagtcg ggcggcaagg tggggcgagg cgggatggct gaagatttca
     8581 ggcatagtca gacgctgttc atcagcttcc tgcatgggta tgcccatgat tttacggtgg
     8641 acggcgatgt cttcggcgat gtcttggcaa ccggtttgca cgggcttttg cgtaatcacg
     8701 cttttgccct gctgcaacaa ttgttttgcc aacataccgg tggcgacggt tttgccgatg
     8761 tccgtgtcta taccgctgac gaagtaaacg cctttcattt gctgtgttcc ttcaagattt
     8821 gcacggtttt atcggcaagt ttggtcaaaa tgccgtctga aatgatatag ggcggcatca
     8881 gatacaccag cctgccgaac gggcgtaccc aaatgccctg cgccacacaa tccgcttgaa
     8941 aacgcgccat atccacgcct ttttccaact ctatcacgcc gatagcaccc aaaacgcgca
     9001 cgtctttcac gccgcgaatg tcccacgcgg ctttcagacg gccttttaag atgctttcga
     9061 tacggcggat atttgcctgc cagtcttggg ataaaagcag cttgaccgaa gcacaggcaa
     9121 cggcacacgc cagcgggttc gccataaatg tcgggccgtg cataaacacg cccgcttcgc
     9181 cgcgtgaaat cgtttcggta actttttgcg aagtgattgc cgccgccagc gtcatatagc
     9241 cgccgctcaa acccttgccg atacacataa tatccggcac gacctccgcg tgttcgcagg
     9301 caaacatctt gcccgtgcgc ccgaatccgg tggcgatttc gtcaaaaatc aacacgatat
     9361 caaattcgtc gcacaaatcg cgcaagccgc gaagatactg cggatgataa aaatacatgc
     9421 cgcccgcgcc ttgcacgacc ggttctaaaa taaaggcggc aatatccaca tgatgcgctt
     9481 caaacaaggc gcggacaggc tgcaaatccg ccccgtccca ttcatcgtcg aaacggtttt
     9541 tcggattatc gacaaaataa cgctgcggca acgcgctgcc gaaaatatgg tgcatccccg
     9601 tttccggatc acaaacggac atcgcgttcc aagtatcgcc gtgatacccg cgccgtaccg
     9661 tcgcaatatt ctgcttcgcc gtcagacccc gcgcctgctg gtattgcacc gccattttca
     9721 acgcgacttc taccgaaacc gaacccgaat ccgcataaaa aatacggtcc agcccctgcg
     9781 gcaaaatccc gaccaacaac ttgcccagct ccaccgccgg ctcgtgcgtc aggccgccga
     9841 acatcacgtg cgccatttgt ttcatctgat tctcaaccgc ctgattcaaa acgggatgat
     9901 tgtaaccgtg tatcgcacac caccaagacg acatcccgtc aatcagccgc gtgccgtccg
     9961 ccaattcgat aaacacccct tctgcacgtt tgacaggata aacgggaagc ggatcggtca
    10021 tggaagtata gggatggagc agatgggtac ggtcgaaatt aagtaatgat gatgtgtgtt
    10081 gatgttcaga cggcataagt ttctctcttt tcttattact gcattcaaac gcaaaaagcg
    10141 tattctactc cgacagaccg cttcccacac ctttccatcc gtttcgggcg aaaaaccgcg
    10201 aaacaaatcg tccgcaacat aagcgcacac cgtttcgcat tcctcaagcc tgattggaat
    10261 cagacgaccc aacgcccaat accgtttcac acagcctccg cccaacgctt caatcaatca
    10321 tagataaata cgatacagat catagcaaca gccatcgcaa cagcgttggc aaaatcaggg
    10381 gactccggca taggcgcata gcatctgccg atgcacggct cctcattcgg ctctatgaat
    10441 accataccca tcacaaaatc cactgctaaa accaggcacg gcttcttata cttatgatag
    10501 atttccacca tcctgtccca tatatcccaa acactcatac cgtatatccc gcaggcaaca
    10561 aattccgatt gaaggttaca gccctatttt atagtggatt aaatttatag tggattaaca
    10621 aaaaccagta cggcgttgcc tcgccttgcc gtactggttt ttgttaatcc actatatttt
    10681 caaaccggaa aaatgccgtc tgaaccttca gacggcattc cattcatatt tattcgtctt
    10741 ttttgtgttc gatggcgttt cgggaacgat tgccgccgta aggctcttca accgtatatt
    10801 ctccctcgat aatatcgtca tcgtggaaaa atccctcttt tctgcccgat tggttcatgt
    10861 tgaaaaaatt ttccgcacct cctgcctgca acactgcccc tcccttaaac ggcagcagca
    10921 gcaataccgc caacaccgag gatacgaatc ccggactcat cagacacacc gccgccaccg
    10981 tataacggat aggccacaac atctgataaa cagatacctt cccactactt tttaccgccg
    11041 cgccagccaa taaaagaccg gacagccccg tatgcctgag catcagcaca ccggcggcaa
    11101 aggttgccgc cattagaaac agcgtccaac cgccgcccag ccaatcggca acccacacaa
    11161 tcgacataat ttccaaaaac agcagcacca aaaaaccgat accgaaaaat ctcattgacc
    11221 gtcatcctta tatttaagta aacagcaaac cgcccgaaca ggattccaag cgagctgcct
    11281 gtaaatgatt acaaaaccat gtgcttcaag ccgaaacaat gtgaaatcac gcaataatta
    11341 tttttagcgt gtaaaacaaa accggctgca tacctgcaac cggtctcaaa tcagcacaac
    11401 tccttatcca aatcctccaa caggtctttc agaccgtctc cgatttcctg aagcgtaacc
    11461 ggacggttgc cgtccgaacc cgattccgca ccttctgcat acggtgcaaa cgtgctcttc
    11521 agtttcaaca gtttcaccac atccaaacgg gtgtagcgtt ctccgccgta accgacaacc
    11581 acaccgtgat catgttgcca ttgcgcaaaa ccataggggc tgatttgcaa cagtccgcac
    11641 aactcgtcca gcgtgaaata gcgttttgca ggaatcgtca aatcaacgtt gtttgtcata
    11701 gtagtgttcc accatgcctt tgagtttctg gctggcatgg aaagttacca cgcggcgggc
    11761 ggtaatcggc acttcctcgc cggttttcgg attacggccc gggcgttgcg gcttgtcgcg
    11821 caactggaaa tttccgaaac cggaaatttt gatttcttcg ccgcttgcca aagtgctgcg
    11881 gatttcttca aaaaagagtt cgacaatttc tttggcatcg tttttggtga cgttgctgac
    11941 tttatccacc aaaatatcgg ccagttctgc tttagttagt gtcatatgat taccttctgt
    12001 tgtaacagtt gtggattatt accaattttt atttaaaatt caagcgaata tttatttttt
    12061 aactgcgaag ccgcgcccct gcctccgttg ccacgccaat cagttttcca ataagcggct
    12121 cgaccgcctc atccgtcagc gtgttttcca tatcctgcaa aatcacttta accgccacgc
    12181 tcttcatccc ttcgggcaga cccgtgccgc gatacacgtc aaacacgctg atttcctgta
    12241 ccaacttgtt tgctgcgcct ttcaagacaa gcagtaaatc atcatgattc ataacttccg
    12301 gcatcacaaa cgccaaatcg cggcgcaccg gctggaattt cgatacggcc cgatagcgcg
    12361 ttttcccgca ttccaacaca gccgccatat cgatttcaaa taccagcggc gcttgcggca
    12421 ggtcgtattt ttgcagccat ttcggatgca gttcgccgac aaagccgatg actttgccgt
    12481 ctgaaacgat attggcggca cgtccgggat gcagggcggg atgtccggtt ttaacgaact
    12541 cgaccgcttt gtttttcaac agattttcca cgtccgcctt gatgtcgtaa aaatccgcat
    12601 tgcgcgtttt ctcaccccat tgttcgggca tcgccgcgcc gtaccacaga ccgccgatgc
    12661 gttcgttttg gacaaaccgg ccgtctgaac ctttgccgaa cacgcaggcg atttcaaaca
    12721 cgcgcacgcg gttttgcttg cggttcaggt tgttttgcaa aatttccact aagccgccaa
    12781 tcaacgtaga acgcatcacg gcatattgcg ccgccagcgg gttttgcagg cggatggggg
    12841 cggcgttggc ggcaaaatcg agttcccact gctcgtcaac gaaggcatag ctgatcactt
    12901 cgcggtagcc gcgcgccgcc atttcgttgt aaacggcaaa acgcggacgg cgtgtttcgg
    12961 gcagttccga cattttcaga cggcctgacg tgtaatcgtc ggggatgttt tcatagccgt
    13021 aaacgcgtcc gatttcttca atcaaatcag cctcaatttc gatgtcgaaa cggaagctcg
    13081 gcgcggtaat gcggaagcct tccgccgttt tctcaggctg cagacccaag tgttgcaaaa
    13141 tggtttccac ctgttcggca ggaatgtcca cgcccaacac ggttttcaga cgacccaaac
    13201 gcaatccgac ctgcttggct tcgggcaatt taccttgtgc ttccaccatc tcgcctgccg
    13261 caccgccgca aatctgcaaa accaattcgg tggcgcgttc aatggcatcc gcctgcaaac
    13321 ggtaatccac gccgcgctcg aagcggaacg acgaatccga accgaaaccg tattggcgcg
    13381 atttgccgac gatgatttcg ggctcaaacc aagccgcttc cagcacgata ttttgcgtgc
    13441 cgtctgaaac cgcgctcgcc gcgccaccca tcaagccggc caagctcaac gcaccttttt
    13501 catcagcgac caccagtgta ttgtcagcca aagtaaccgt cttctcattc agacacgcca
    13561 aagtctcgcc attttgagca cggcggacaa tcaaactgcc cgacagctta tcagcatcga
    13621 aaacatgcat aggctgaccg atttccagca tcacatagtt gccaatgtcc accaatgcgg
    13681 aaatgctgcg gataccgctg cgttccaaac gttgtttcat ccaatcagga gtagccgctt
    13741 tcgcgttgac attttcaata acgcggctga taaaacggcc gcagtcagcc ggtgcatcaa
    13801 tacggacagc ctgttttttc tcactgccga taggggccgt ctgaatttca acaggcgtaa
    13861 acgcacattg agtcaacgca gaaacctcac gcgcaatgcc cttaacactc aagcaatcag
    13921 cgcggttagg cgtaattttc aacgtaaaca gcatatcgtc caaatccaag tattcgcgga
    13981 tgttggtacc gacaggcgca tcttcaggca gaatgtgcag gccgtctaca ccatcatcag
    14041 gcaaacccag ttcattggtc gaacacagca taccgtttga tggtacgccg cgcattttag
    14101 tcggcttaat tttaaaattg cccggcaaaa cggcaaccgg caacgaacac ggcactttaa
    14161 tgcccggctt gacattcggc gcaccacaaa caatctgaac caactcgccc gtaccggcat
    14221 caacttgagt aacgttcaaa cggtctgcat caggatgttt ttcaacagat tttacttcgg
    14281 caacaaccac gccactgaaa gcaggggcgg cagtatcgat ttcttccact tccaaaccgg
    14341 ccatggtcaa aagatgttcc agtttatcgg cagaaagatc aggattggct tgggttttca
    14401 gccatgagta ggagaattgc atgttatgtt ctcaatcaat taattaactt ttgttttatc
    14461 aattttcgtt cagacagtct gttttagaat tctggttaat gttgatattt actcaactct
    14521 gaaaaatatt ttctctgtga aattttaaaa attccagact tgggtttagt ctttcaggca
    14581 gtcttatcat ccgattttca tatggtttaa ataaatcatc aaatccttct gtttttgcca
    14641 gtagtggaga aaatataatt ttgaaatttt catctattcc taacaacccc ctatcaaaag
    14701 ctttgtcatg taacgcattc aaacatagcc cattccttgg gtttaaacgg ttatccttat
    14761 cttcccccca gggtttgata tgactagcaa ctaacaattc aggttgtttt aatccagtaa
    14821 tacaacattg attattgtat gctgccaaaa cactggaacg gaaaaagccc tgattgacgc
    14881 gtactgatat tcttgcaaac ctttcccttc cctttggaat ctcaggtgat tctatttcaa
    14941 tattactatt ctcatcattt gattggtatt gggaaatgac tccctcaaat tctttattaa
    15001 gttgctccca atctcctgaa aactcattcc atacgacaac atccatttta ctgccattac
    15061 ttaatcccga aatatttttg actttcagcg taggatctaa acgacccaaa tttcctatct
    15121 tcataccgag tgcggacggt gtcctgccca gtatttcagc atattcttga ataacaggat
    15181 tgtccttact aaccttttga aaaggaatga tgtaataaag ataaagggca accagggttt
    15241 catctctagt ccattttttc atttttatca gctatctttc tgattttcca taaggaaatc
    15301 gtacaataca gattttctta ttccctgaac atattgctgt ccatctagta ctaatttgta
    15361 taggtataca tcataatctt ttaactcctt ctcgcttgcc aataaccagt cgtctatttc
    15421 tgaagaaaaa tagtcaataa tttcttgtac ctgtttatca gataaattat ttatatttat
    15481 tttattattc aaaatataat cagaaagcct catatttcta aaatctttat tttgataagg
    15541 taaacccaca cagcacatca aattgcttcg gaatgtattg tttgatttct tccaaagtaa
    15601 tatcgccaaa cggcacttct ccgaaattgg cttcgtacgt cagtttggct ttttcgtccc
    15661 actcgctcga aaacacgtat tccccgccca agttctgcat cgcgatgcgg aagcccccga
    15721 tacctgcgaa caagtcgata aaggtaaatt tgggtttcaa ggccgtctga aaagtatcgt
    15781 tgttttcaaa aaggcggtat tgtgctgctt tttcttttaa ccgttcatcc tgcccatctt
    15841 ccttaatctt gtttttgtaa atcaattcat cttgtttgga tgatttcttt gactttttcg
    15901 gcacagcagg ttgtattttt gacgacatcg ttactccaaa aacgtaaaac cgtccagccc
    15961 tcggctttca gacggcctgt tacttctata tccctttgga tattgcgctc aattttggca
    16021 atccaaaact cacgatttcc ctttattacc gcttttttct gttcccaatc cttaccgtgc
    16081 cagaattccc catcgacaaa tacggcaact ttatattttt taaatgaaaa atcaggtttt
    16141 ccaaaaatgc ttccgctatt tttccgatac ctgagcccca aagcccacat tgccttcgcc
    16201 aacacaagct cgggtttcgt ccctgtactt ttgttggact gcatacattt tttgcgctgc
    16261 tccggggtta atttatccat tgggtttatt tgagatttat tcaaactgct tcaagaagtt
    16321 caaatcgtta tcgaagaaca agcgcaggtc attgacgttg tagcgcaaca tggcaaagcg
    16381 gtcgagacca ataccgaaag cgaaaccggt atatttttca gggtcgatat tgacattttt
    16441 caacacatta ggatgtacca taccgcaacc gcctacttcc aaccatttgc cgttttctcc
    16501 catgatgtcg atttcggcag aaggctcggt aaacgggaag aaagacggac ggaaacgcac
    16561 ttgtaggtca tcgcgttcaa agaagcgacg gataaagtca gtgaacactg cttttaagtc
    16621 ggcaaaagtt acgccctctt ctacccacaa accctcggcc tgatggaaca taggcgagtg
    16681 cgtggcatcg ctgtccacac ggtaaacgcg gccgggggcg ataatgcgga tgggcggctc
    16741 ttttttatcg agcatatagc ggatttgaat cggggaagtg tgcgtacgca aaacatcgcc
    16801 gttttcaacg taaaacgtat cctgcatcgc acgggcagga tggtttgcag ggatgttcag
    16861 ggcttggaaa ttgtgaaaat cgtcttcgat ttcaggcccg tccgccactt cgaaacccat
    16921 tccgtgaaag agttcgacca cacgttgcaa ggtcagggtt acgggatgca ggctgccgcc
    16981 ttcctgagcg cgtccgggca gggtaatgtc gagggcttcg gcggcaagtc gggcttgcag
    17041 cttggcttcg ttgagggcat cgcgtttggc attaaaagcc gtctgaaacc ggtttttgca
    17101 ttcattgata tgcgcaccta tggttttgcg ctcttcaggc gacatttgcc ccaaagtttt
    17161 cagaagtccg gtcaactcgc cggttttgcc aagataacgg gctttgattt gttctagagc
    17221 gttgaagtct tgcgcagctt ctactgcggc aatgccttct gcaacgatac ggtttacatt
    17281 ttccataata tcaagcctgt caattaatga tatgccacct gaaacaacga ggccgcctgt
    17341 atagcctgac atatcagact cttcagacga ccttattttc ttcacggcca atccattgga
    17401 taaattttct gtattttaac gcaaatttat ttaaaaaaat agactttaaa gaaagaaaat
    17461 caatcacata gaatttccgc aaacaaagaa aaaaggaagc cgcagcttcc ttttcaattt
    17521 tttggattaa gcagccaaag cagctttggc tttttcaacc aattgtgcaa aagcggcttt
    17581 atcgaacacg gccaaatcag ccaatacttt gcggtcgatt tcgatagagg cgcgtttcag
    17641 accgttcata aatttgctgt aagacaaccc gttttcacgc gcacctgcat tgatacgaac
    17701 aatccacaat tgacggaatt ggcgtttgcg ttggcggcgg tcacggtatg cgtattgacc
    17761 agctttcatt accgcctgct tggcaacgcg gtaaacgttt ttacgacgac cacgatagcc
    17821 tttggctaac gcgaagattt tttggtgacg ggcacgggcg gtaacaccgc gttttacgcg
    17881 tggcatattc taaactcctt aagcgtaggg taacatttta gcaacagaag ccaaatcgcg
    17941 atcatttacc atagaggtac cgcgcagttg gcgtttgttt ttggtggtct ttttagtcaa
    18001 gatgtggcgt ttgaacgcat gagcgcgttt cacaccgccg ttacccagta ctttaaagcg
    18061 ttttttcgcg ctagacttgg ttttcatttt aggcatggga aaactccatt cgttatcgga
    18121 taaggcatta gggggtttta aaacatcggt ttcaaacact tgaacccaca atgccacggt
    18181 tttttgccgc aattgaaaac ttactccgaa gcggcaatcg gagtaagcgg aaaattatag
    18241 ctttattttt tcttcggcgc aatcatcatc accatttgac ggccttccat tttgggaaag
    18301 gactcgattt gcgccacttc agccaaatct tcttttacac gttccaaaag ttgcgcgccg
    18361 agttgctggt gagccatttc acggccgcgg aaacgcaatg tcactttgac tttatcgccg
    18421 tcggcaagga agcggttgat gttgcgcatc ttgatttgat aatcgccctc atctgtaccc
    18481 ggacggaatt tgatttcctt gatttgcacc tgcttttggt ttttcttggc ttcgtcgcgc
    18541 ttcttggctt gttggtattt gtatttaccg taatccatca gtttgcacac aggaggttta
    18601 gcagttgggg aaatctctac caaatcgaca tcctgccctt cggccatagc caaagcttca
    18661 cgaactgaaa cgacaccaag ctgttcgcct gactcactga ttaaacgcac ttctttggcg
    18721 gtaatttcac cgttgattcg tgcttcgcgt tcttgagcga tgatgaatac tcctataaaa
    18781 attaatgatt gacgagggca tcagtgattt cttgctgcaa ttgcgcaatg aaatcatcca
    18841 aatccaaaga acccaagtct tccgctttgc ggcgtaccgc aactttgttt tcttgtttct
    18901 ctttatcgcc gataacgatt tgataaggga aacggtattg gctgttgtcg cggattttgt
    18961 aaccgatttt ttcattacgc aaatccaact cggcgcggaa gcctgccgcc tgcaatttgg
    19021 cagcaacttc ccgacaataa tctgcctgat tttcggtgat gttcataatc accatttgaa
    19081 ccggtgcaag ccataacggg aaagagcctg catggttctc aatcagaatg ccgacaaacc
    19141 gctccaaaga acctaaaatg gcgcgatgca acataacagg ccgcgctcgg tcgttgtttt
    19201 ctgttacata ttctgcatcc aagcgttccg gcaagacgaa atccagttgt aatgtaccgc
    19261 attgccaaga acgacccaag gcatctttga cgtgatattc gattttaggc ccgtaaaacg
    19321 caccctcgcc cggcaattcg ccccattcca cgccgcaggc agtcaatgcc tcgcgcaaac
    19381 cctgctctgc cttatcccac acgtcgtctg aacctgcgcg tttttcaggg cgaagggaaa
    19441 gcctgacgga tacatcatgg aaaccgaact gtttgtagat gcgaaccaac aattcattga
    19501 acgcacgagc ctcgctgacg atttgatctt cggtacaaaa aatatgcgca tcatcctgca
    19561 caaaaccgcg aaccctcatc agaccgtgca gtgcgccgct cggctcgttg cggtggcaag
    19621 aaccgaattc cgccaaacgc atcggcaaat ctcgatatga acgcaaaccg ttgttgaaaa
    19681 tttgaacatg acccggacag ttcatcggtt taaccgcata ttcgcgtttt tccgaactgg
    19741 ttacgaacat attgtctttg tagttgtccc agtggccgga tttttcccaa aaggttttat
    19801 ccataatttg aggcgttttg acctctttat aaccggcggc gttcagctct ttacgcatat
    19861 gctgctcgat agtccgccac aaagcccaac ctttgggatg ccaaaacacc atacccggcg
    19921 cttcgtcctg caagtggaac aaatccagtt gcttgcccaa tttgcggtgg tcacgttttt
    19981 ctgcttcttc aatgcgttgg atatagtctt ttaattcgtc tttcgtcgcc caagcagtgc
    20041 cataaatacg ttgcagcatt tcattattgc tgtcgccgcg ccaatatgcg cctgccagct
    20101 tggtcagctt gaagtttttc aggaagcgga tgttcggaac gtgcggaccg cggcacatat
    20161 cgacatattc ctgatgatga tacataccca ttgcttcaac ttcgggcata tcttcaatca
    20221 ggcgcagttt gtattcttcg ccgcgctctt gaaaaatttt aatcgcctcc gcacgcggag
    20281 tcatgatttt gaccacatca taatcttggg caatcaattc tttcatacgc gcttcaatgg
    20341 cggcaacatc ttccggtgta aacggttttt ccgtggcgat gtcgtaataa aagccctctt
    20401 caatgacggg gccgataacc atttttgcat taggatagag ttgcttgacg gcatgcccga
    20461 caagatgtgc gcaggaatgg cggatgattt cgatgccttc ctgatctttc ggagtaatga
    20521 tttgaacagc agaatcctca acaatcgggt cgcacgcatc gaccaatttg ccgtttacct
    20581 tgcctgccac cgccgccttc gccaaaccgg caccgataga cgcagcaatt tgagccacgg
    20641 taacggggga ttcgtattgg cggactgaac agtccggcaa ggtaatattc aacatcaaac
    20701 gctccgaaag attaaaaaat aacggcataa atgccgttat gggaatttgg taggcacgat
    20761 tggattcgaa ccaacgaccc ccaccatgtc aaggtggtgc tctaaccaac tgagccacgt
    20821 gccttcaaag aatttttgta ttttatcggg tcgttttaat ttttgcaaga gagatgtgca
    20881 tttctttatg caacattgtg gcaagatttg gggataccgt ctgaaaaacc gtgggaattc
    20941 atttataatg ccgtctttta aaatatattt cgacccctgt cgcccaactt acattcaatc
    21001 ttatgcttaa atttacttta cacaaaaaag acggtctcgc ccgacgcggc acgctggagt
    21061 tgaaccacgg aaaaatcgaa acgccggttt tcatgccggt cggtacttac ggttcggtca
    21121 aagcgatgaa cccgcaaaac ctgcacgaca tcaaggcgca aattattttg ggcaacactt
    21181 atcatttgtg gctgcgtccg ggtttggagg tcgtcgaaca gtttggaggg cttcacggtt
    21241 ttatcggctg ggacaaaccg attctgaccg actcgggcgg ttttcaggtt ttttctttgt
    21301 cggatatgcg caagctgacc gaagaaggct gtacgtttaa aagcccgatt aacggcgaca
    21361 agctgttttt atcacctgaa atttccatga aaatccaaac ggttttgaac tcggatatcg
    21421 cgatgcagtt ggacgaatgc acgccgggcg aaacaacgcg cgaacaggcg cgaaaatccc
    21481 tgcaaatgag cttgcgctgg gcggaacgga gcaaaaaagc ctttgaagat ttgaaaaacc
    21541 cgaacgcgct gttcggcatc gtgcaaggcg cgatgtatga ggatttgcgc gaagaatctt
    21601 tgcgcggttt ggaggaattt gattttcccg gccttgccgt cggcggtttg tccgtcggcg
    21661 aacccaagcc cgaaatgtac cgtatgctgc acgccgtcgg cccgatgctg cccgaacaca
    21721 aaccgcatta cctgatgggc gtaggcacgc cggaagacct cgtgtacggc gtggcgcacg
    21781 gcatcgatat gttcgactgc gtgatgccca cccgcaatgc gcgcaacggc tggctgttta
    21841 cccgtttcgg cgatttgaaa atcaaaaacg ccaaacacaa gcccgacaag cgtccgatag
    21901 acgaaagctg cacctgctac gcctgccaaa acttcagccg cgcctacctg caccatctgc
    21961 accgcgccgg cgaaatcttg ggcgcgcaat tgaacaccat ccacaacctg catttctacc
    22021 aagtcattat ggcggaaatg cgcgatgccg tcgaacaagg caaatttgcc gactggcagg
    22081 cgcaattcca cgaaaaccgc gcgcgcggcg tggactgatt cccggcaatg ccgtctgaag
    22141 ccgcttcaga cagcattttg ccgctttacc ctacctgcac tatttttcag aaaccgatca
    22201 tgcttccatt ccttccagaa ccctcccttt cctatacgca acaaaaccgt accgccgttt
    22261 tgctgttgaa cctcggcaca cccgacgcac cgaccgcaca ggcggtacgc ccttatctga
    22321 aatcctttct gaccgaccgg cgcatcgtcg aactgcccaa atggctgtgg tatcccattc
    22381 tgcatggcct ggtactgacg ttccgcccca aaaaaagcgc gcacgcctat gaaaaaatct
    22441 ggtttaagga aggttcgccc ctagaagttt acacagcacg ccaagctgcc gcgcttgccg
    22501 aacgtatgcc cgacctcatc gtccgccacg ccatgactta cggcaatcct tcgatagcgg
    22561 atgtattggc ggagctgaaa tcccaaggtg taggcagatt gcttgcgatt cccctatatc
    22621 cgcaatatac cgcttccagc agcggagcgg cggtagataa agtgtgcgaa caactgctgc
    22681 tgcaacgcaa ccaaatgagc gtccgcacca tttcgcgctt ttatgatgat gccggctaca
    22741 ttgatgcgat gaaaaaccac atcctccgat attgggcgga acacgggcgt gggaaaaaac
    22801 tgatgttgag ttttcacggc gtaccgcaga agcactacga cctcggcgac ccctatcccg
    22861 acgagtgccg ccacaccgcc aaactgcttg ccgaagcact ggaactgacc gaagacgaat
    22921 ataccgtatc gttccaaagc caattcggca gggcaaaatg ggttacaccg agcacgcagg
    22981 atttgttcgg caaactgccc aaacaggggg taaccgagct ggacgtattc tgcccgggct
    23041 ttttggcaga ctgcttggaa accatggaag aaatcgccct gacggggcgg gaacagtttt
    23101 atgaagcggg cggaaaaaac taccgctaca tcccctgcct caacgacaac cccgactgga
    23161 tagacgcact cgtcgcactt gccgaagaaa accttggcgg ctggcgttga aactgtttga
    23221 gccatgtcta aaatgccgtc tgaacgccgt gttcagacgg cattttcccg ctactttagc
    23281 gtatgatggc atctatgccg gacagcacat caagtgcaac cttactaccg attcggcaaa
    23341 aaaacctttc acccgctacg caaaatcaac aacaaggttt caaacattaa ggaaacgcca
    23401 atgaacacaa cccgactgcc gaccgccttc atcttgtgct gcctctgcgc cgccgcttct
    23461 gccgccgaca acagcatcat gacaaaaggg caaaaagtgt acgaatccaa ctgcatcgcc
    23521 tgccacggca aaaagggcga aggccgcgga accatgtttc cgccgctcta ccgctccgac
    23581 ttcatcatga aaaaaccgca ggtgctgctg cacagcatgg tcaaaggcat caacggtaca
    23641 atcaaagtca acggcaaaac ctacaacgga ttcatgcccg caaccgccat cagcgatgcg
    23701 gacattgccg ccgtcgccac ttatatcatg aacgcctttg acaacggcgg cggaagcgtt
    23761 accgaaaaag acgtaaaaca ggcaaaaggc aaaaaaaacc aaacagacaa aatgccgtcc
    23821 gaaaccggca atccggcttc agacggcatt caaaccaaac ctttctaacg cggtactttc
    23881 agacccatag acccgatatt gccgtccccg tcaacctcct tcgcggtcag cataaagccg
    23941 ccgatgtcaa tacggtcgcc ctcgaccggc tcctgactgt cggaacgctt atcgaaaagc
    24001 tcggcaaggc tcaaaccctc ttcgcccgct tccagcttca aaccgtaagc aagtgccaaa
    24061 tcgcccgaac gtgctgccgg cgaaacgaca aactcaccga agaaatcaaa attttcacgg
    24121 acgccgatac ccgtctcggt aaagtatttc gccattttat cgaccttgcc gtcgggcagg
    24181 atgtaccacg ccaaatcctc cgcctgaagc acggtgtcgg actgcatttc gattcggctg
    24241 ccgttgcgta tcaatgcgaa acagcgtgcg tcaaacgaat cggaaatcgg ttcgaccgta
    24301 tcgggatggt gtccctccgc ttcagattcg gcaaccactt taaacgccga cattctgaca
    24361 atttcctttt ccgccagcca aatatcatgc gtatctttcg gttcgggctt gttgggcatg
    24421 gcaaccttca acagccgcgc catcacagga atcgtcgttc cctgaatcag cagcgacagc
    24481 accaccacgg caaacgccac atcaaacagc aggtgcgaat tgggaacgcc catcaccagc
    24541 ggcatcatcg ccagcgaaat cggtacggct cctcgcaagc ccaaccaact gatatacgcc
    24601 ttttcacgca ggctgtaatt gaatttccac aaaccgccga acactgccag cggacgcgcg
    24661 accagcatca ggaacgccgc aatcgccaag gcttccgccg ccctgtccaa cacgccggcg
    24721 ggagaaacca gcagaccgag catgacgaac aaagttgcct gcgccagcca agccaaaccg
    24781 tccatcacac gcaaaacgtg ttccgtcgca cggttgcgct ggttaccgac aatgatgccg
    24841 gcaaggtaaa ccgccaaaaa gccgctgccg cctatggtat tggtaaacgc aaacacaagc
    24901 agcccgcccg acacaatcat cagcgcgtac agaccttccg caagattcag acggcgtacc
    24961 agctttgcca atatctttcc gcccgcccaa cccgtcagca gaccgaaacc gatttgcagc
    25021 gcaagcatcc ggacaaacgc tgccgcaccc gattccgccg gctgcataat catggtaatc
    25081 agtgccgtaa ccaaaaaaac cgccatcggg tcgttcgcac ccgattcgat ttccaaagtc
    25141 gcctgcaccc gttcgttcaa acgcacgccg ctgttgcgca aaaggctgaa taccgcgccg
    25201 gcatcggtcg aaccgacaat cgccgccatc agcacgccga acttccaatc caaaccgaga
    25261 taaaaagttg caaacagtcc cagcggaagc acggtggcaa acacgcccca cgaagcgagt
    25321 accgacgcgg gcttcaacgc aatccgaaaa ctggaaagct gcgtccgcaa accgccgtcg
    25381 agcagaataa ccgccaaagc aagctggctg atgaaattcg ccatcacgac attattgaag
    25441 gcaatgccgc caacgccttc ctcgcccgcc agcatgccga cggcaagaaa caccagcagc
    25501 aaaggcattc ccaaacgtgc ggacaaagtg gtcgaaacca cgctcaggaa caacagtgcg
    25561 ccacccagca gaaacaggct gttcatccaa tccacgccaa cggctccccc ttaaaattca
    25621 tgaaattaga atcgaaagat tgtaacacgg cattttcttc tcacgaaact ttatttcatg
    25681 tccgccaccg ccttcagacg gcattttcct gtttcatgcc gcctgatacc gcccaaaccc
    25741 gcgttctcct atataatgtc ggactttgcg ccgcatttca ggatttttta tggcatttgc
    25801 ctcactattt actctgccgg actacatcac cgccgttttg gacgatgttg ccctgatgac
    25861 caaagccgcc gcaaagaaaa ccgccggcgt ggccggcgac gatttggcac ccaatgccaa
    25921 ccgggttaca ggcgtatcgg cagaacgcga gttgccgatt gtttggccgg tggcgaaagg
    25981 ttcttttgcc aacaagccgg ttttggtgtc tgcggctctg ctgctttccg ccttcctgcc
    26041 gcaactcatt acgccgcttt tgacggcagg cgggatttat ttgtgctttg agggcgtgga
    26101 aaagctgctg cacaagtttc tgcaccgaca tgacgcgcac gatgacggcg gcgaaacggc
    26161 ggttaacgag caggctaaaa tcaaaggtgc aatccgtacc gactttatcc tgtccgcaga
    26221 aatcatcatc atcgcgctgt gcgtggtcga acaatacagc ctgatgacgc gctcgctcgt
    26281 gatggcggca atcggcatcg gcatgactgt tttggtgtac ggaatcgtcg ccgtcatcgt
    26341 caaactcgac gacttgggca tgctgctgat gcgccgccca caacctcggc cttttgcacg
    26401 atttcctgca cgcgcaacac tggaacgctg gctggacaga gtattccgcc aacctcgttg
    26461 tcgggccgct ttccggttcg attgcctgcg ccccgccttg tcgctgatga atcgtttcgg
    26521 caggcattga ttcctttttc aaataccgat gccgtctgaa agacgttcag acggcatatt
    26581 ccgaacagac agacgaatat gttttccaaa ctggacaaat actggcagca ccccgccctc
    26641 tactggcctt tgctcatcct ttttgccgcc gccaccccct tcgccttcgc accctactac
    26701 cacttttggc tgatgccctt gattttcggc gccttcgtcc gcctcatcga actgcgtccg
    26761 cgttttgccg cctcttccgc ctacctgttc ggtctgaccg catacacggc acagttctac
    26821 tggatacaca ctgccctgca cgacgtttcc ggcctgcccg acctctatgc cgtaccgctg
    26881 accttcctgc tgcccgccta ccttgccctt tatccggcac tgtgtttctg gctgtggaaa
    26941 aaatttaccc tgccccaggg cataaagacc ggtctggtac tgcccatcct gtggacgctg
    27001 accgagtttg cccgcgagcg tttcctgacc ggattcggct ggggcgcaat cggctactcc
    27061 caaatcaccc cggacagccc gctggcgggt ttcgccccct tgggcggcat ccacatggtt
    27121 acactggcaa ccgcctttct cggtgtctgg ctggttttgg cgatcgacaa caccgcacgt
    27181 tcgggcaaac gcctgcttcc ggccattctg attgccgccc tgcttgccgc aggctacacc
    27241 gcccaacaaa ccgacttcac ccgccccgac ggcagccgca gcaccgtcgc cctgcttcag
    27301 ggcaacatcg cccaaaccct caaatggcgt gaagaccaag tcatcccgac catacagaaa
    27361 tattacgaac aggtcggcaa aaccactgcc gacatcgtca tcctgcccga aaccgccatt
    27421 cccatcatgc gccaaaacct gccggaaaac atactggcgc aattcgccga acaggcgcaa
    27481 aacaacggca gcgcgctcgc cgtcggcatc agccaataca cttcggacgg caacggttac
    27541 gaaaacgccg tcatcaacct gaccggctat caggaaaaca atcaggacgg catcccttac
    27601 tacgccaaaa accacctcgt ccccttcggc gaatacaaac cgctgcccct cctgaccaca
    27661 ccgctttaca aaatgatgga catgcccctt tccgacttcc gcaaaggcgg cggcaagcaa
    27721 tccgccatga tgatgaaaaa ccaaaaaatc gccttcaaca tctgctacga agacggattc
    27781 ggcgacgaac tgattgccgc cgccaaagac gccacactgc ttgccaatgt cagcaatatg
    27841 gcgtggtatg gaaaatccaa cgccatgtac cagcacctcc aacaatcgca ggcgcgggct
    27901 atggaactcg gacgctatat ggtccgcgcc accaacaccg gcgcaaccgc catcatctcc
    27961 cccaaaggca acatcatcgc ccaagcccaa cccgatacgg aaaccgtatt agaaggacac
    28021 atcaaaggct acatcggcga aacgccctat atgaaaaccg gcagttcatg gtggttgatg
    28081 ggcatattga ccctagccgc actgattctt ttcatcttcc gaaacaaaga acactgatac
    28141 ctgcacagtc cgtccggacg ggctgtcttt tttcaaaccg gccgtttaca tacaaccacg
    28201 tatgtaatat aattctaaaa tcgaattgag ctatcaggtt tacaaaatca atcaaacttg
    28261 ccttacaaaa ttttcctgtc tttcaaacct tagcgtatat ctcctgccaa acatacaaaa
    28321 tacaagccaa gcagtgatat actgcatccc aacacaccaa accgccgata cgcagtttca
    28381 gccgaaagga aatgccccaa atgaataacg ctttcgcatt acccgccatc caaagcggca
    28441 acggcagcct cgaacaatac atccacaccg tcaacagcat cccgatgctg tcccaggaag
    28501 aagaaacccg cctcgccgaa cgccggataa agggcgacct caacgccgcc aaacaactca
    28561 tcctgtccca cctgcgcgtc gtcgtttcca tcgcgcgcgg ctatgacggc tacgggctga
    28621 atcaggccga cctgattcag gaaggaaata tcggtctgat gaaggcggtc aaacgctacg
    28681 agcccggtcg aggtgcacgc ctgttttcat ttgccgtgca ttggattaaa gcggaaattc
    28741 acgaattcat cctgaggaac tggcgccttg tccgcgttgc caccaccaaa ccgcaacgca
    28801 agctgttttt caacctgcgc agcatgcgta aaaaccttaa tgtcctgtct cccaaagagg
    28861 cacaagacat cgccgacgat ttgggtgtca aactttccga agttctggaa atggaacagc
    28921 gcatgacggg acacgacatc gccatcatgg cagacaacag cgacgacgag gacagtttcg
    28981 cccccatcga ctggcttgcc gaccacgatt ccgaaccgag ccgacaacta tcgaaacagg
    29041 cgcattacgc cctgcaaacc gaaggcctgc aaaacgcctt ggcgcaattg gacgacagga
    29101 gccgccgcat tgtggaaagc cgctggcttc aagacgacgg cggactgacg ctgcaccagc
    29161 ttgccgccga atacggcgta tctgccgagc gcatccgcca gattgaagcg aaagccatgc
    29221 agaaactgcg cggtttcctg accgaagaag ccgaagcggt ttgaaatata cacaataaaa
    29281 tacccgacaa aacaatttgt cgggtatttt atttggtaaa catcaatgcg gtttgtgcgt
    29341 cgtgccgtac acgattttat ccgcccaagc catcgcaatc gccaaaatca aaaagcagac
    29401 atgcaccaga cactgccaca tcagctgctt ttccgacaga ttggcggaat tgataaatgt
    29461 ttggagtaaa tggatggatg aataccgata atcgacatcg acagccttac cttcaatacc
    29521 ggtgcattca catggctcaa ccactcgggc cggtcaggat ggtcgtcgat acgcaaccgg
    29581 gatacgaacg actcataccc gccaatctgc accatggtca gcaaattcgc aatcataacc
    29641 acatcaatca gatttaaaac agcgagcatg atggcgtttt cgtccgacac atccaaattc
    29701 atgaccagat acttcaacga tttcaaaaac ttataggcac aaatcgcccg tacaacagtc
    29761 agccccgcat aaatcggcac ttgcagccaa cggcttgcaa aaatcatttt ggcaaaaaac
    29821 ccatagcctg ttcgggtttt ggagattttg cttgatacat ttcccttcct tgcagcaaca
    29881 aaagcgcgtg attccaatct accgctgcga aatcttcaat ttttcacacc cgaaaacgct
    29941 tgccgccata cagatgtgat atattgattc cattttaacg aacttcggag aacgacatga
    30001 gcagcggaaa tatccgcatc cacagcatga ccggctttgc caacgcggcg gcagagtgcg
    30061 gcagcaaacg catcaacctc gacatccgcg ccgtcaacca ccgctttttg gacattcaaa
    30121 tcaggatgcc cgacgatttg cgccatttgg aaaacagcat ccgcgagcag atttcatccc
    30181 atctggcgcg cggcaaagtc gaatgcaaaa tccaaattca agatgcggaa aacggcaatc
    30241 agtctttgga actgaatcgc gttctggtcg gacagcttgc cgaaatcaac aaaaacctcc
    30301 gcaaacatta tgatttggca aaactgggcg ttgctgatat tttgcgcttt ccgggcgtat
    30361 tggcaagcca aaaggaaaat acggaagcat tggcgaaaac cattgtcgaa ctggccgaca
    30421 aagcactaaa agactttacc gccgcacgtc ggcgtgaagg gaaaaaattg ggcgaacacc
    30481 tgttacaacg cattaaaaac atggaagaaa tcatcgatgc tttgagcgaa atcttccccg
    30541 cgctggtgga agcgcataaa gaaaaaatcc gcacccgcct tgccgaagcc gtcggcagca
    30601 tcgacaacga caggctgcaa caggaatttg ccctctttat ccaaaaatcc gacatcgacg
    30661 aagaattcag ccgcctgcgc acccacattg ccgaagtgcg ccgtatcgtt accgaacaca
    30721 aaggcagcag cggcaaacgg ttggacttcc tgatgcagga actgaaccgc gaagccaaca
    30781 ctttgggcag caaatccatc gctaccgaat gcacccaagc ctcggtcgag ctgaaagtcc
    30841 tgatcgaaca gatgcgcgaa caggtgcaga atatcgaata gccctgcctt tctctccaaa
    30901 catgacaatg ccgtctgaaa cttttttcag acggcattct tgatcggctg aaaacctctg
    30961 ttgcaagcaa ttcaagattg ttctttcaca tccaccaacc tcgttccggc aaggaagtca
    31021 tacagaaact gccggtcggg attcagcagg gcgaaccccc acggcaaaat cagccaaacc
    31081 aatgccgcgc cggccgcgcc cttgggcgga atgccgagga agtggcgcaa tccggcataa
    31141 gcaagcatag gaatgaagac gatgaatatg cacgcccaaa taaagcgcag gcgcagcagg
    31201 tgcaaagacg gctgtatgcc gttaaggtcg cacaagccga ttttccatgt cctcatcgcc
    31261 aaggtctgcc cctgaccgtg ccagttggcg cggaaataaa gccaccatgc tcccattatc
    31321 aggatactcg ttaccaatgc agaaaccgcg atagaaacgg gattcagaaa aatggcggca
    31381 ataccggcaa gcaatgctgc caaacaggtc gccgcaccga ccagcagcat ctcatacagt
    31441 atggcggcaa agcggcgttt gagcggggcg gttttcagta tcatcatctt ttcaatcgga
    31501 atatgccgtc tgaaacgcca acagacttca gacggcattt gtgcaaagat gccttgcggc
    31561 ggttaacggt gcggacgttt ctgaccgcgc gactgttttg ccgccatttg attgatttcc
    31621 tgacgtgtcg ggcgtttttt gaacatagcg tgcgcctcgt ccaccgaaca tttttgcgac
    31681 aggcagatca gggtaatcgc atctttctgg tgttgtccgc cgctgtggta catacggcgc
    31741 aggtttgcct cgttgattct gcctttttcg ggatcgatgt cgaatttttt cagacgttcg
    31801 ataaactggc gttgctgtcg gcgggatgtc caaacaaagc cgcccgcagc ggcggcgacg
    31861 atgacggcgg caagcaggat ggcaagcggc caggaatcga caatcaatac gataatgagg
    31921 cttaaaacgg cggtggtcag taaaatcagg ccgcccaagc gcagtttggt cttattgtcc
    31981 atagggttga tgttccttta ttgaggtggt cgtgccgtct gaaatgtttt cggacggatt
    32041 ggtgaatact tttcaggatt aacggtattt tacagtattt tcggattttg gggattaccg
    32101 ctttattccg ccagcgacac gacaagctgt ttgaataccg tccatgcgtc gccgtcttcc
    32161 gcgcctttga tgattcggtc gatttgggcg caggttttga gcgcgtcaag caggcggacg
    32221 acggaaatcc gcttgaccgc aagcggtgcg agcgtctgtt tgtcgcccca aagcctgagg
    32281 ctgttgcgga cggattggat gctctgcccc tgtttcaggg cagcggcaag ccgaatcagc
    32341 gtccgcacgt cttcggcaac cgcccacaga agcagcaccg gttcttcgcc ttcttcctcc
    32401 aacccgtcca acagcctgca tacgcgcggg acatcgccct tcatccacgc gcctgccagt
    32461 tggaacgcgt cgaagcgggc aacattggca acggcggttt gcgcctcatc gatattgacg
    32521 gcatgccctt tcggatacag cagcgcgagc ttgtcgattt cctgacgtgc cgccaacaga
    32581 ttgccttcca cgcgctcggc aaacagtgcc aatgcgtctg cctcgatacc caaaccgatt
    32641 ttgtccagcc gtccgcgtat ccattggggc aaagccgccg caccgaccgg tttggcttcc
    32701 cacacttccc cctttgccgc caatgcggca aaccatttgg actggagccg ggttttctcc
    32761 agtttgggca gcaaaaccag cgttaccgta tcttccggca gtcgggcggc aaaatcctgc
    32821 aatgcctcgc cgccgttttt gccgggtttt ccgttgggga tatgcaattc caacagtttc
    32881 aaatcggcaa acagacccgc actgcctgcg gtttgcagca gttcgttcca atcgaaagac
    32941 gcgtcggcgg tataggcttc gcggttgaga taaccctgct tcttcgccgc cgccctcaat
    33001 gcgtccactg cctcgatacg cagcagttct tcctcgccgt ggatgacgta cagaggtttc
    33061 aaaggcgcgt ccgtatcgat gcgtccgata tgtgccgcca cgtcattccg ccttcagaaa
    33121 ggtcaggcgg cggacaatct gttcggcaac atcctgccgc atttccgccc acagggtttc
    33181 ttcttcttcc tgtttgccca aaatttcgtt gtcggcataa tccaaaatgc ggcggacgga
    33241 cacggtcatc ggtttgccga ccggctcgcc gcgtttcaat acctgcgctt caaccgtcaa
    33301 tatcaaaaga tattcgttga tgactgccgc acgggtaacg gtataggttt ccttgttttg
    33361 ggaaacgctg tctatacgca gggtcatctg cgcgccggca gcatcgtcca ccctgcccga
    33421 agcctgatac agcgcggttt ccaaaggaaa ctgcaatgcc tgtccgcctt cgatgtgcca
    33481 gctccggtag gtcagcggcg gagaaatgcc gtctgcacct ttcaggtgga aaccgcacgc
    33541 gcccagcacc aaggctgcgg cagtaaggaa tattttgttc atcgcttgcc tttctcaaaa
    33601 gcataaagtc ggtagattat atcagcggcg tgttcagacg gcattgccgt ccagccaaat
    33661 caggctcgcc atacgccccg tcgctccgtc gcggcggtag gaaaagaaaa tatcccgttc
    33721 caaaaccgta caatgcgtgc cgccgtacac gccgcccacg ccttcgcgcc tcagaatcag
    33781 gcgggcgagc gcgtagaggt cggcaaggaa tttgccgccg ccgatgggtt caaatgcagc
    33841 agcggcttcg ggcatggacg tgcagaacgc atcaaacacg tcctgcccga cttcaaacgc
    33901 atccgcactg atggcagggc cgagatacgc cataatttcg acaggcggaa ccttcatcgc
    33961 ggcaatggtg ttttgcaata cgccgcccgc caaaccgctc cagcccgcgt gtgcggcggc
    34021 aaccgccgta cctgccctgt cgcaaaatag aacgggcaga cagtctgcag tcatcacggc
    34081 acagacggct ttacccgtgt cgtccacgga agcgtcggca tcgggcgtgc cgtccaatgc
    34141 ttcggcagca ttgacgacaa tggtgctgtg gatttgattg agataggcaa cgggcaatcc
    34201 gacctgctgt tgcacgattt cgcggttgcg gcgcacggtt tcagggtcgt cgccgacgtg
    34261 cgtaccgagg ttcaaactct gatacgcacc ttggctcacg ccgccgttgc gcgtggtaat
    34321 cagggttttc acattggcgg gcgcgggcca atcggcggtc aggaagtttt tgcctttcgg
    34381 ggcgagattt agggtttctg tgatggtttt cattggatat tggaataggt gtgcggcgga
    34441 aagggttgat taactgcctt cggggcaaat ggatgcttgt tttttcgtca ttcccgcaca
    34501 ggcgggaatt cggagattcg ggattgtttt caaacgttcg ggtcggattc ttgccggtgc
    34561 gggaatgatg cccttatata ctttctccga gctttatatg cttcaacagg ggacggcaca
    34621 tcaagcaccg cactgcgtgt tgcccgaacg aggctgccct ccgattagat tctatcgcta
    34681 taaacagacg ggtttcaacc gaaaaggaat ggggataaag tccatttccg acacctctcg
    34741 ggcgatgccg tctgaaaacc aatctccact ttcagacggc attgtttagg cgcggtcatt
    34801 ttgcaaatta aggttaaggc gtgtatgctt gccttccctt aaaaacaaac acgcaacgca
    34861 ttattatgaa tatcctcagt aaaatcagca gctttatcgg aaaaacattt tccctctgaa
    34921 cctcgctctt tgccgccgcc gcttttttcg cgcccgacac cttcaaatgg gcggggcctt
    34981 atattccttg gctgttgggc attattatgt tcggtatggg tttgacgctc aaaccttccg
    35041 acttcgatat tttgttcaaa caccccaaag ccgtcatcat cggcgtaatc gcacaattcg
    35101 ccattatgcc ggcaaccgcc tggctgccgt ccaaactgtt gaacctgcct gccgaaatcg
    35161 cggtcggcgt gattttggtc ggctgctgcc cgggcggtac ggcttccaat gtgatgacct
    35221 atctggcgcg cggcaatgtg gctttgtcgg ttgccgttac gtctgtttcc accctgattt
    35281 ccccattgct gactcccgcc atcttcctga tgcttgccgg tgaaatgctg gaaatccaag
    35341 cggccggtat gttgatgtcc atcgtcaaaa tggttttgct ccccattgtt ttgggtttga
    35401 ttgtccataa ggttttgggc agtaaaaccg aaaagccgac cgatgcgctg ccgctggttt
    35461 ccgttgccgc catcgtgctg attatcggcg cggtagtcgg ggcaagcaaa ggcaagatta
    35521 tggaaagcgg cctgctgatt tttgcggttg tcgtactccg caacggcatc ggccacctgc
    35581 tcggcttctt tgccgccaaa tggaccggcc tgccttatga tgcacaaaaa acgctggcca
    35641 tcgaagtcgg tatgcaaaac tcgggcctgg ccgccgcgct tgccgccgca cactttgccg
    35701 ccgcgccggt cgttgccgtt ccgggcgcat tgttcagcgt gtggcacaat atctccggct
    35761 cgctgctggc aacttattgg gcggccaaag ccggtaaaca taaaaaaccc taaacgcctt
    35821 tcatcataat gccgtctgaa cggggcaacc gaaaccggcg gtttctgccc ggccccttca
    35881 gacggccttt gtttgaaatc ggcggggacg attgcccggc tgctccggtt cggctgcctg
    35941 cgttcgcccc gcctttcaag ccgcctcact cccttacata aaccacttcg acatcgtagt
    36001 cgtcttcgtt ccaatcgtca tcgtcgtccg cgccgaattt gtcctgccat tcctcttcat
    36061 tgctcaaaga cgaatccaaa ccggcttcaa gacggaggac ggacaacaaa tgatatatgt
    36121 cgtccggaat cggtgcctca aacgaaacgg tttcgccgct ttccggatgg gtaaaactca
    36181 agcggtaggc gtgcaacgcc tgacgcgcgc ccaaactttt aacggcttct ttcaccgtgt
    36241 cgccgcacgg atggcgcggg ttgccgtaaa ccgggtcgcc ggcaagcgga tggttggcct
    36301 cgcgcatatg gacgcggatt tggtgcgtcc tgcccgttcc gagcgagcat tcgatgtagc
    36361 tgtgggcaag atagcgttcc aacactttga cgtgggttac ggcaggcttg ccgccgaatt
    36421 tgacggctgc cattttcagg cggttgtgcg gatcgcgtcc gatttgggtt tcgattttac
    36481 cgtcaaaggg gacgatgccg ttggcgacgg cgcggtagat gcgtttgacc gtgcgttctt
    36541 gaagctgcct cacgagggaa ttttgcgccg gcagggtttt ggcaaccacc atcagcccgc
    36601 tggtttcttt gtccaaacgg tgtacgatgc ccgcgcgcgg aatttgggac agctcggggc
    36661 aatgcgccaa caggccgttg agcagcgtcc ccgtccagtt gcccgccgcc ggatgcacca
    36721 ccagtccggt cggtttgttg acgacgatga cggtatcgtc ttcgtaaaca atatccaaat
    36781 ccatagactc cggaacaaac gccagatttt cctcactcgg acggacggtt acacaaattt
    36841 gctcgccgcc tatcattttg tctttgggtt gcgaaggttt atcgtttaca ataaccgcgc
    36901 cttctttaat ccatgatgtc aggcggctgc gcgaatagtc gggcagaagt ttcgccaaca
    36961 ccgcatccaa ccgcccgccg gcaagctcga gcggaacggt cagaccgata cggttttcag
    37021 ttccggaagc tgacgcaaag tctgaatcgt cgctataatc ggattcatta tcaaaggaag
    37081 tattctgcat gaaaaaaatt cttttaacgg tttcattagg tttggcactg agtgcctgtg
    37141 ccactcaagg tacggccgat aaagatgccc agattaccca agattggagt gtggagaagc
    37201 tctatgccga agcccaggac gaattgaaca gcagcaatta tacgcgggct gtcaagttat
    37261 acgaaatctt ggaatcgcgc ttccccacca gccgccatgc ccggcaatcc caactggata
    37321 ccgcatacgc ctattataaa gacgatgaaa aagacaaggc tctggcggca atcgaacgct
    37381 tccgccgcct ccatccgcag catccgaaca tggattacgc gctgtacctg cgcggcttgg
    37441 tgctgtttaa cgaagatcag tccttcctga acaaactggc ctcgcaagac tggtccgacc
    37501 gcgacccgaa agccaaccgc gaagcgtacc aggcgtttgc ggaactcgtc caacgcttcc
    37561 cgaacagcaa atacgccgcc gatgcaaccg cacgcatggt caaactggtc gatgctttgg
    37621 gcggcaatga aatgtcggtg gcgcgttact acatgaaacg cggcgcatat atcgccgccg
    37681 ccaaccgcgc caaaaaaatt atcggcagct atcaaaatac acgctatgtc gaagaatcac
    37741 tggccatatt ggaacttgcc tacaagaagt tggacaagcc ccagcttgcc gccgatacgc
    37801 gccgcgtatt ggaaaccaac ttcccgaaaa gcccgttttt gacgcacgct tggcagcccg
    37861 acgatatgcc ctggtggcgt tactggcatt aagcccatcc tcgaaatgcc gtctgaagcc
    37921 ctttcagacg gcattttccg ttccggcacc agccttcccg ttcactcaaa cggttttttc
    37981 tgccaataga atttatacac tctcaaacgt tgagccttta cgcctccgcg tcccaagccg
    38041 aattgcagcg cgcccgacaa atccgtccgc aacaacttga tgccgtgtgc gcggacacgg
    38101 ttctgcaccg cttcggtcgg atgcttgtag gcgttggcat aaccgcttga agcaacggca
    38161 tattcgggcg aaacggcatt gaggaacacg cccgacgagg acgtattgct gccgtgatgc
    38221 cccaacacca acacctggct gtacaggttg cctccatact tgccgaccag gctttcctcg
    38281 cccttcgtat ccaaatcgcc cgttaccagc agtgccgcac cgcccgccac aacacgcaaa
    38341 acacaacttt tcccattatc atcgatattt ttgcgttcag acggcctcaa aaactcgaaa
    38401 tccacgccgt cccattgcca acgctgttcc gcacaatgcc gcgccccctc ataaaattcc
    38461 ggctgtccgg cataaatccc gccgttcggt atcttgccga ctgcctgaaa gccgccgtcg
    38521 tggtcgctgt cgtgatgcga cagaaccagc ttgtccaaac ggcggacacc cgccgcattc
    38581 aaactcggca cgatacccgt ttgtgccgcc gctaccgttc ccgtatcgaa aagcagatgg
    38641 cggtttgccg tccgcacaag caccgacaag ccctgccccg catcccaaac cgtaaccgca
    38701 gcttcgtttt caggtactgc ttcaggacgg taggatacaa accctgccaa cagcaacacc
    38761 gcccacggac gcaaacccaa gccgcgcggc agcagcaaca gcaaagcggc acacaccgcc
    38821 aacaccagca gcggcaaagg tgcggcggca acggcaaact caggcgacac atcggcaagc
    38881 cacaccaaaa accgcaaagt gtattccgcc aaaaatgccc ccgcctgctg aagcggcgca
    38941 aacggcacga ccgaacccag caacgccagc ggggtcaaca cccaagaaaa ccacggtatc
    39001 gacaccgcat tgaccaaagg gctgaccaaa ggcagcgagg caaacagata accgagcaaa
    39061 accagagaca acaccgaagc cgcccactgc ccgcgcacgg cggtttgccg tttcccctcg
    39121 tacaaacgcc ctgcacacgc ccatatcagg gccgccacca aaccgaaaga cagccaagta
    39181 ccgacaccca aaaccgccaa agggtcgaac agcagcaccg ccgccaacgc ctgccaccac
    39241 gtcgcccacg ccgacagtct tcccctgcgc cacgcccacg cgaacgccgc caacatcaaa
    39301 acactgcgct gcgtcggcac ggaaaaaccg gcaagcagcg cgtaaaacag cgcgcccgca
    39361 caccccgccg ccaaaaccca cgcgcgcggc cgggcgggaa ggcgcgggga acaggcaagc
    39421 agccgcttcg ccagccacgc aaacagcacc gccaccatcg taacgtgcaa acccgagata
    39481 ctgaccaaat gcgtcagccc caacggccgg aacgcctgcc acaatccggg gcgcaatgcc
    39541 gactgttcgc ccacgctcaa cgcgcgcatc agcccgatgc cgtctgaaag cccgccgtcc
    39601 gcatccgcct gccgccaatt gcggctgatg cggctgcgcc aaaccgcaat cccccaaccg
    39661 cttccgccat gcagcaaaac cctgtccgca ccgaccgtcc ccacgccgcc taccccgttg
    39721 gataatgccc acgcctcacg gttcaaaccg cgcaggttca actccccgac gacggggtgc
    39781 acgcgtgccg ttatccgcca tctgctcccg accgcccatt cgcgccgttt gtagtccgac
    39841 agcagcaaat caaacgtccg accgccgctg tccacagcct ttgccgcaaa ctgcacgcgc
    39901 cgcccgtccg acctcggcat atccgtcact tccaccgtca aaggcacgcc tgaaaccgcc
    39961 tccgcccgcc attgcgaaga cagtgccgct tccgttctga atacgccgta agccgcgccc
    40021 gccaacacgc acagcatcag accggcaaac gcaaaacgcc ttgcaagcac agccaaaacc
    40081 gcaaaagccg ccaaccaaaa cggccaatgc ggtacaaccg acagtgcgaa cgaaactgcc
    40141 acgccgacaa cccaacacgg caaaaaccaa cgacggaaca cgtctcccca ctcccgatat
    40201 aaaaaacaaa cgatgataaa gcaaagccat aaaaccgtca taacaagatt tgaaaatgat
    40261 aagaatgcaa acgaaaagcc tcagtattat ccgagacaac ggcaaacttt ccatcgtcat
    40321 tcccgcaaaa gcgggaatcc aaagacaaaa agccgcagaa atttatcgga aagaaccgaa
    40381 ccacaaaaac cggcttccca ccttcgcttg aataacagaa caaagaaagc ggaaataaaa
    40441 acatcaggat tcttttttaa attggcaata aaacaccaaa atgaatgagt tacacaaaaa
    40501 aatactcaac accacccaac cggcgtaaaa tgcaaaacat tatcgctatt aaaacggtaa
    40561 aaccttatga aagccaaacg ttttaaaatt aacgccatat ccttatccat ctttcttgcc
    40621 tatgccctta cgccatactc agaagcggca ttggtgagag acgatgtcga ttatcaaata
    40681 ttccgtgact ttgcagaaaa caaaggcaaa ttttttgtcg gcgcaaccga tttatcagtg
    40741 aaaaacaaac aaggtcaaaa catcggcaac gcgttatcca acgtaccgat gattgatttt
    40801 agcgttgcag atgtcaacaa acgcatagca acggtagtcg atccccaata tgccgtcagc
    40861 gtcaaacacg ccaaagcaga agtccatact ttttattacg gccaatacaa cggtcataat
    40921 gatgtagccg acaaagaaaa tgaataccgc gttgtcgaac aaaataacta tgaaccccat
    40981 aaagcttgga gtgcgagcaa cttaggccgc ctcgaagatt ataatatggc gcgttttaat
    41041 aagtttgtaa ccgaagtggc accgattgca ccaactgatg ccggcggcgg attagatact
    41101 tacaaagata aaaaccgttt ttcctctttt gtcagaatcg gtgcgggcag gcaattagtt
    41161 tatgaaaaag gggtttatca tcaagaagga aatgaaaaag gctacgattt gcgcgatctt
    41221 tcacaagcct atcgttatgc cattgcaggt acgccttata aagatattaa tattgaccaa
    41281 acaatgaata ccgaaggttt gatcggcttt ggtaatcata ataaacagta ttcggcagaa
    41341 gaacttaaac aagcactttc gcaagatgcg ttaacaaatt acggcgtgtt gggcgatagc
    41401 ggctctccgt tatttgcttt tgacaaacaa aaaaatcaat gggtcttttt gggaacttac
    41461 gattattggg caggttacgg aaaaaaatca tggcaagaat ggaatatcta taaaaaagaa
    41521 tttgcagata aaatcaaaca acacgataac gccggcaccg tcaaaggtaa tggagaacat
    41581 cattggaaaa ccacgggtac aaacagccat atcggttcga cagcggtaag gcttgccaac
    41641 aatgaaggag atgcgaacaa cggacaaaat gttacctttg aagacaacgg cactttggta
    41701 ttggatcaaa acatcaacca aggcgcgggc ggcctgtttt tcaaaggcga ttacacagtc
    41761 aaaggcgcaa ataatgacat cacttggtta ggtgcgggga ttgatgttgc cgacggcaaa
    41821 aaagtcgttt ggcaagtcaa aaatccgaat ggcgacagat tggcaaaaat cggcaaaggc
    41881 actttggaaa taaacggcac aggcgttaac caagggcaat taaaggtcgg cgacggtacg
    41941 gttattctga atcaaaaagc cgatgccgac aaaaaagttc aggctttctc ccaagtcggc
    42001 attgtcagcg gacgcggtac attggtatta aatagttcaa atcagattaa tcccgataac
    42061 ctatatttcg gtttccgtgg cggtcgtttg gatgccaatg gcaatgactt gacttttgaa
    42121 cacatccgca acgtggatga aggcgcgcgc attgtcaacc acaacacaga ccacgcctcc
    42181 acaatcacac taacgggtaa atctttaatt accaatccga acagcctttc ggtacatagc
    42241 atacaaaatg actatgatga agatgattat agttattatt accgaccaag gcgacccatc
    42301 ccacaaggca aagatcttta ttacaaaaac tatcgttatt acgccctaaa atccggcggc
    42361 aacgtgaacg caccgatgcc cgagaacggt gtagcagaaa ataacgactg ggtctttatg
    42421 ggatatacgc aagaagaagc caagaaaaac gcgatgaacc acaaaaacaa tcagcgtatt
    42481 agcggtttta gcggtttctt tggcgaagaa aacgaaaaag gacataacgg ggcattaaac
    42541 cttaatttca atggcaaaag cgcgcaaaac cgtttcttgt taacaggcgg cgcgaattta
    42601 aacggaaaaa taagcgtaac tcaaggcaat gtcttgttat caggtcgtcc aacaccacac
    42661 gcaagagatt ttgtgaacaa atcttcagcc cgaaaagacg cacatttctc caaaaacaat
    42721 gaagtcgtat ttgaagacga ctggataaac cgcacattca aagccgcaga aattgcggtt
    42781 aaccaatccg catcattctc ttccggaaga aatgtatccg acatcaccgc caacatcacc
    42841 gcaacagaca acgccaaagt aaatttgggt tacaaaaacg gcgatgaggt ttgcgtgcgc
    42901 tcggactata ccggttacgt tacctgcaac acaggcaact tatccgataa ggctttaaac
    42961 agctttgatg cgacacggat taacggcaat gtgaatttga gtcagaatgc ggcattggtc
    43021 ttgggcaagg ctgcattatg ggggcaaatt caagggcaag gaaacagccg tgtcagccta
    43081 aaccaacata gcaaatggca tttgaccggc gacagccaag tacacaatct gtcattggcg
    43141 gatagccata ttcatttgaa caacgcttcc gatgcgcaaa gtgcaaataa ataccacacg
    43201 atcaaaatca atcatttatc cggtaacggg cattttcatt atctgacgga cttggcgaaa
    43261 aatcttgggg ataaagtgct tgtgaaggaa tccgcatccg gccattatca gctccatgtt
    43321 caaaataaaa caggcgaacc taatcaggaa gggctggatc tctttgatgc atcatccgta
    43381 caagaccgct cccgcctttt tgtttccttg gcaaatcatt acgtcgattt aggcgcattg
    43441 cgttatacaa tcaaaacaga aaacggtatt acccggttgt acaatcctta tgccgggaac
    43501 cgccgcccag tcaagccggc cccgtctcct gccgcaaaca cggcttctca agcacaaaag
    43561 gcaacacaaa cggacggtgc acaaattgcc aaacctcaag atattgtcgt cgcaccgccc
    43621 agcccgcagg caaatcaagc cgaagaagca aaacgccaac aagcaaaagc ggagcaagtg
    43681 aagcgtcagc aagcagaagc agggcgtaaa tcggcagagt tgtcggcaaa acaaagggcc
    43741 ggggaagagg aacgccgtca aacagcccaa tcccagccgc aacgccgcaa acgccgtgcc
    43801 gcaccgcagg attatatggc agtttcccaa gaccgtccga aacgccgcgg acaccgttcg
    43861 gtacagcaaa acaatgtgga aattgcccaa gcccaagcag aacttgcgcg ccgacagcag
    43921 gaagaacgca aagcggcaga gttgttggca aaacaaaggg cagaagccga acgtgaggct
    43981 caagcattgg ctgcgcgccg aaaagccgaa gccgaagagg caaaacacca agctgccgaa
    44041 cttgcacatc gccaggaagc aaaacgcaaa gcggcagaat ccgcgaaacg aaaagctgag
    44101 gaagaagagc
//
LOCUS       DAWTQS010000007        43692 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.007, whole genome shotgun sequence.
ACCESSION   DAWTQS010000007 DAWTQS010000000
VERSION     DAWTQS010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 43692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43692
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.007"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(277..1062)
                     /locus_tag="WNA25_000408"
     CDS             complement(277..1062)
                     /locus_tag="WNA25_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HFA9823242.1"
                     /translation="MNPFLNTAFKAARRAGQMMIRAAGNLDAVKTDSKAFNDFVSDVD
                     RNSEIILVEALKEAYPHHKITCEESGFHGKATAEYEWIIDPLDGTTNFLHGHPQYAIS
                     MALLHKGVLQEALVYSPERNDVYMASRGKGALLNDRRIRVSNRIELNRCLIGTGFPVV
                     DQSMMDKYLAILKDFLAKTAGGRREGAASLDLCAVATGRFDGFFEFNLKPWDIAAGAL
                     IVQEAGGIVTDMSGEDAWLESGDIVAANPKVLAQMLKIISAHV"
     gene            1306..2121
                     /locus_tag="WNA25_000409"
     CDS             1306..2121
                     /locus_tag="WNA25_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HFA9823243.1"
                     /translation="MTALKPALPDYLGNIRIILTRTSHPANIGSAARAMKTMGLHRLT
                     IVTPNLMATPMTENPPVFNPDDVQSFALPEESFILASGAADVLHNAEIVATLDEALAD
                     TTIACALTSRRREITAPLQTPRDLVPELLQAANRGEKVALVFGNETFGLSIEEVRACN
                     RLMTINGNPDYFSLNLAQAVQVVCYEIFSQTDSPMTHLQQEDHAATHEQIKGMLAHME
                     SVMDDIGFFNRRNGERLMRRMQSLFGRANTQTEDIDILRGFFNTVSHRIHKKD"
     gene            2162..3031
                     /locus_tag="WNA25_000410"
     CDS             2162..3031
                     /locus_tag="WNA25_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1853 family protein"
                     /protein_id="HFA9823244.1"
                     /translation="MTDIRISMNYALDALWWKLTSQPVRDLASLLTAPPLWQSGCELS
                     VRELLGERGFRYLLALDADPAPLTDYLAQRAPFDHRLGIYAEELLAFWFTNAPHTKLH
                     AYNLTVFSDGQTLGAADFVVSLNQQPYHIELACKYYGGDQVQNLRGLNPKDTLTDKAA
                     KLVQQFQLVHTSQGKATLAAQDLPENPLPASIVRGIGFFPQGFHAFEPPLNPYGWRGI
                     YIQDWAEYGFERQEARYHLLDRMVYLAPARVAETETLNATEIRRIDQGLIAVLECRPD
                     GFWHEIERIMKAV"
     gene            3067..4323
                     /locus_tag="WNA25_000411"
     CDS             3067..4323
                     /locus_tag="WNA25_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HFA9823245.1"
                     /translation="MNAAQLDHTAKVLAEMLTFKQPADAVLSAYFRKHKKLGRQDRHE
                     IAETAFAALRHYQKISTALRRPHAQPRKAALAALVLGRSTNISQIKDLLDEEETEFFG
                     NLKARKTEFSDGLNTAAELPQWLVEQLQQHWSEEEILAFGRSINQAAPLDIRVNTLKG
                     KRDKVLPLLQAESPDAEATPYSPWGIRLKNKIALNKHELFLDGTLEVQGEGSQLLALL
                     VGAKRGEIIVDFCAGAGGKTLAVGAQMANKGRIYAFDIAEKRLANLKPRMTRAGLTNI
                     HPERIGSEHDTRIARLAGKADRVLVDAPCSGLGTLRRNPDLKYRQSAETVAKLLEQQH
                     SILDAASKLVKPQGCLVYATCSVLPEENELQIKRFLSEHPEFEPVNCAELLQNLKVDL
                     DTGKYLRLDSARHQTDGFFAAVLQRK"
     gene            4669..5097
                     /locus_tag="WNA25_000412"
     CDS             4669..5097
                     /locus_tag="WNA25_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HFA9823246.1"
                     /translation="MNFALSVIAFTLASFLPVPPAGAAVFTWKDGGGNSYSDVPKQLH
                     PDQSQILNLRTLQTKPAVKPKPAVDTNADSAKENEKDIAEKNGQLEEEKKKIAETERQ
                     NKEENCRISKMNLKAVGNSNAKNKDDLIRKYNNAVNKYCR"
     gene            complement(5146..6489)
                     /locus_tag="WNA25_000413"
     CDS             complement(5146..6489)
                     /locus_tag="WNA25_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFA9823247.1"
                     /translation="MFHSVNVFTGETLYRRPDQDYAEFERRLADLKMHGRAFAQLGVT
                     ERAARLQKFAGRLEAEKERFAEMVCEEVGRCLHECRAEIVKSIELIRYYARLAPELLA
                     HKTIATQASLSQVRFEPLGVVFAVMPWNYPVWQVLRFAVPAMCAGNACAVKPAPSVAR
                     VSRALFDLASDGIPLAGAWLDEADTLKAVEDTDAMAFTGSTHTGRILAAHAGANLKKT
                     VLELGGSNAFIVMPDADLERAAAEACYSRFRDAGQSCNAAKRIIVTEAAADRFIQLFL
                     AECAKLKMGDPKHPDTTLAPLHREDLRDRVHGQVEDAVSNGAVCLTGGKVPQGSGWFY
                     PATVLDRVNPACRVWREEVFGPVALILRAENEEHAICLANDSPFGLGACIYTADTERA
                     WRFAEKIQAGSVFINRHTSSDLRLPFGGVKDSGYGRELSEFGLYEFVNVKTYWQK"
     gene            complement(6494..7147)
                     /locus_tag="WNA25_000414"
     CDS             complement(6494..7147)
                     /locus_tag="WNA25_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HFA9823248.1"
                     /translation="MGLGMEIGKLIVAFLVLINPFSALSLYLDLTNGHSTKERRKVAR
                     TAAVAVFAVIAVFALIGGALLKVLGISVGSFQVGGGILVLLIAISMMNGNDNPAKQNL
                     GAQPETGQARPARNAGAIAVVPIAIPITIGPGGISTVIIYASAAKTYSDIALIIAAGL
                     VVSAICYAILIVAGKVSRLLGATGLTILNRIMGMMLAAVSVEIIVSGLKTIFPQLAG"
     gene            7297..7587
                     /gene="gatC"
                     /locus_tag="WNA25_000415"
     CDS             7297..7587
                     /gene="gatC"
                     /locus_tag="WNA25_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFA9823249.1"
                     /translation="MALTLADVDKIARLSRLQLTAAEKEKSLQELNDIFAMVEQMQTI
                     NTDGIEPMTHPHEAALRLREDEVTETDRAVEYQAVAPEVRNRLYIVPQVIEE"
     gene            7650..9095
                     /gene="gatA"
                     /locus_tag="WNA25_000416"
     CDS             7650..9095
                     /gene="gatA"
                     /locus_tag="WNA25_000416"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFA9823250.1"
                     /translation="MTQYTLKQASVLLQSKQISAVELASAYLAAIAEKNPALNGYITI
                     DQDKTLAEARAADERIAQGNASALTGVPVAYKDIFCQTGWRSACSSKMLDNFVSPYTA
                     TVVQNLLDEGMVTLGRTNMDEFAMGSTNENSFYGAAKNPWNLEHVPGGSSGGSAAVVA
                     ARLAPAALGSDTGGSIRQPASHCGITGIKPTYGTVSRFGMVAYASSFDQAGPMAQTAE
                     DCAILLNAMAGFDPKDSTSLEREKEDYTRDLNQPLKGMKIGLPKEYFSEGNSTDVQTA
                     LQHTIDLLKAQGAELVEVSLPQTKLSIPAYYVLASAEASTNLSRYDGVRYGHRAAQFG
                     DLEEMYGKTRAEGFGSEVKRRIMIGTYVLSHGYYDAYYLKAQKLRRLVADDFQTAFAR
                     CDLILAPTAPSAAPKIGADASPVETYLSDIYTIAVNLAGLPALTLPAGFSDGGLPIGV
                     QLIGNYFAEAKILGAAHQIQLNSDWHGKRPE"
     gene            9092..10024
                     /locus_tag="WNA25_000417"
     CDS             9092..10024
                     /locus_tag="WNA25_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFA9823251.1"
                     /translation="MKQNRTFTFPDFRTVYSYAPLYRLQHLKYTLRKFFGKKEIYAFE
                     QFVNASPIRQGLFLHCPQDAYPLLREFVDRRFNCKRRLDAMTADFLMAEKLFGTDILH
                     QMEDYRFHLVLAHLSDGISLWLNRNDNCVEEGAWSLSLRDEAGNRLYMATFAFVGTHL
                     LAASVQGPSGEEAKDTVRRITKQLHGLRPQQLMVTALQYFAAALKLDGAIGIAQKHQV
                     KLRWKLKKRVKMNYDTFWQEYGASLERDGYWHLPQTPARKDLADIESKKRSMYRKRYE
                     MLDNMVAEMKDSLKTEARGISDGIQTEKPLRRTA"
     gene            10067..11497
                     /gene="gatB"
                     /locus_tag="WNA25_000418"
     CDS             10067..11497
                     /gene="gatB"
                     /locus_tag="WNA25_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFA9823252.1"
                     /translation="MTWETVIGLEIHVQLNTKSKIFSGASAAFGSEPNAHASVVECAL
                     PGVLPVMNREVVEKAIKLGLALDAKINQKNVFDRKNYFYPDLPKGYQISQLDLPIVEH
                     GKLEIVVGDDVKTINVTRAHMEEDAGKSVHEGLNGATGIDLNRAGTPLLEVVSEPEMR
                     SAAEAVAYAKALHSLVTWLDICDGNMAEGSFRVDANVSVRPKGQEDFGTRREIKNLNS
                     FRFLDQAINYEAEAQIEILEDGGTVQQATMLFDPEKGETRVMRLKEDAHDYGYFPDPD
                     LLPVIISDAQMQKAKAEMPELPKEMAARFVADYGVSEYDARLLTASRVQAAYFEEAAK
                     ESGQGKPTANWMNGELAATLNKEGMELADSPITAPRLAALVGKIADGTLSGKLAKKAF
                     EAMWAEPETSIAEIIEKHSLQQMTDTGAVEAMVDEVLANNAKAVEQFKSGNEKALNAI
                     VGQVMKTSKGKANPAQVQELIKAKLA"
     gene            11589..12599
                     /locus_tag="WNA25_000419"
     CDS             11589..12599
                     /locus_tag="WNA25_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HFA9823253.1"
                     /translation="MNHTVTLPDQTTFAAGDGETVLSAAARQNLNLPHSCKNGVCGQC
                     KAELASGDIQMGGHSEQALSEAEKAQGKILMCRTTAQSDININIPGCKADALPVRTLP
                     ARIESMVFKHDVALLKLALPKAPPFAFYAGQYIDLLLPGNVSRSYSIANSPDQEGILE
                     LHIRRRENGVCSEMIFGSEPKVKEKGIVRVKGPLGSFTLQEDSGKPVILPATDTGYAP
                     IRSILLDLIRQNSSRAAHFYWGARHQDDLYALEEAQGLACRLKNTCFTPVLSRPGEGW
                     QGRKGHVQDIAAQDHPDLSEYEVFACGSPAMTEQAKNLFVQQHKLPENLFFSDAFTPS
                     AS"
     gene            complement(12729..13361)
                     /gene="pdxH"
                     /locus_tag="WNA25_000420"
     CDS             complement(12729..13361)
                     /gene="pdxH"
                     /locus_tag="WNA25_000420"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFA9823254.1"
                     /translation="MDLHNIREDYSKRELSEADCADNPIEQFERWLDEAVRAEVNEPT
                     AVNVAAVDGRGRPNSRMVLLKEVNSEGFVFFTNYHSRKGRSLELNPFAAMTFFWPELE
                     RQVRVEGRVGRLAEKLSDEYFESRPYQSRLGAWASAQSEVIPNKAVLVAKAAAVGLKH
                     PLHVPRPPHWGGYIVIPDLIEFWQGRPSRLHDRIQYRLLDGGWIRERLSP"
     gene            13421..13573
                     /locus_tag="WNA25_000421"
     CDS             13421..13573
                     /locus_tag="WNA25_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020424398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823255.1"
                     /translation="MHKLCPQSRSDDFIRKTAASGTISNRPTEDGILSTRPAAHAAEE
                     PPYQAS"
     gene            13582..14631
                     /locus_tag="WNA25_000422"
     CDS             13582..14631
                     /locus_tag="WNA25_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HFA9823256.1"
                     /translation="MSKQPTSKRQWRDGAAPSAKKTAKPFKSKARPKDETRKTAAQAY
                     GQKASDGIKLQNAPKQRAAKAKKLVVRNPNQKIMEHARDLKERRSDLSRMEPERLQKV
                     LAASGVGSRREMEEWINNGWVTVNGKTAQLGDKVTPDDHVTVKGSIIKLKWADRLPRI
                     ILYYKQEGEIVSRDDPQGRVSIFDRLPQAASSRWVAIGRLDINTSGLLILTTSGELVQ
                     RFAHPSFEVEREYAVRVLGGLTTEQMRSLTEEGVMLEDGLAKVERIYEQGGEGANKWY
                     NIVIKEGRNREVRRIFESQGLTVSRLVRVAFGPIGLPNRLKRGQFYELNPAEVANILK
                     WADMLLPGERRRKKA"
     gene            complement(14690..16231)
                     /locus_tag="WNA25_000423"
     CDS             complement(14690..16231)
                     /locus_tag="WNA25_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HFA9823257.1"
                     /translation="MKFLDREASIAKPGFNRWLVPPAALAVHLAIGQIYAYSVFNAPL
                     TKLIGITESAAGDWKLTTVGWIFSIALAMLGASAALFGTWMERVGPRKAIFAAACCFS
                     LGFFVSAFGVRTHNLFLLYLGNGVIGGVGLGLGYIGPVSTLMKWFPDKPGMATGLAIM
                     GFGGGAMLASPLSVSLMNAFSNAASVGVAETFAVLGLFYLALMMFGAFTIRVPADGWK
                     PEGYTVPKTQNKPVSSNHVNVSQAMKTPQFWLLFWVLCLNVTAGIGVLGQASVMIQEL
                     FSETSAGRQAAVGAGAAAGFVSLLSLFNMGGRFLWSSVSDKIGRKNTYTIFFVLGSLL
                     YFAVPSIGEGGSKALFIIGFCVIISMYGGGFAAIPAYLKDLFGTYQVGAIHGRILLAW
                     SIAAVIGPVLVNYIRQSQIDSGIPAAQAYSVTMYIMAGLLIIGLLCNLAVKSVHEKHH
                     EKDIKTAARSGNPDDETAISDAYLVGEKVSGDGISVWWRWALAVIPLAYGVVMVFVKA
                     LDLFS"
     gene            complement(16490..17845)
                     /locus_tag="WNA25_000424"
     CDS             complement(16490..17845)
                     /locus_tag="WNA25_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFA9823258.1"
                     /translation="MSDFFHSDVLSVSELNAFAKSILENHLAGLWIAGEVSNLTRAAS
                     GHYYFSLKDSRAQVRCAMFKGAAARLAQPLKEGDHIEVAGKISIYEARGEFQITVNEV
                     RLKGLGQLYEAYERLKAQLQAEGAFAAERKKPLPVRPQCIGIVTSLAAAALRDVVTTL
                     KRRAPEIPVIVYPAAVQGAGSGFQIAQAIKTASQRAECDVLIVCRGGGSIEDLRAFNE
                     EPVVRAIEACTIPVVSGVGHETDFTLADFVADVRAPTPTGAAELVSPNRQESLHRLVQ
                     AQGRLKTVLEQRYFDASQKLDWLARQIRHPRQKLDEQRASIGKLAQTLSYSMTQNLRA
                     HTARFERQTQALQHCRPDVSVYRQDIVRLQTALPAAFSRLLARRRQSLTAQAALLEAV
                     SPQHILERGFSVVKNTRGQVIRNADVLKQGQKLHITFSDGETDVRVSKEQGQQDLFDC
                     I"
     gene            18042..18863
                     /gene="nadE"
                     /locus_tag="WNA25_000425"
     CDS             18042..18863
                     /gene="nadE"
                     /locus_tag="WNA25_000425"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HFA9823259.1"
                     /translation="MMRIFSTGGKMDTQAVITHIVRWLDEYAAQANAKGFVVGVSGGI
                     DSAVVSTLAARTGRTTLLLDMPIRQHPGQLERARRHIRNLQGQYANVSAQTANLTDTF
                     QTFEQTVGVHRTAFANQPLSLANARSRLRMLTLYYYGQIHGLLVTGTGNKVEDFGVGF
                     FTKYGDGGVDISPIADLTKTQVYRLAEALGVDEAIQKAPPTDGLWDTERTDEEQMGAS
                     YPELEWAMGVYGTRKPEDFEGRRREVLEIYTRLHRAMQHKINPIPVCRIPPELLG"
     gene            complement(18934..19407)
                     /locus_tag="WNA25_000426"
     CDS             complement(18934..19407)
                     /locus_tag="WNA25_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein DnrN"
                     /protein_id="HFA9823260.1"
                     /translation="MTDFSVWEAAPFGATVDHILQRYHNVHRAQFEELVPLAQKVAQV
                     HADTFPAEIAGLLADMRDELLMHMMKEERMLFPMINQGVGRGAAMPISVMMHEHEEHD
                     RAIARLKELTGNFHAPEGACGSWTRLYALAKEMADDLNDHIHLENDILFARVLDS"
     gene            19705..20037
                     /gene="trxA"
                     /locus_tag="WNA25_000427"
     CDS             19705..20037
                     /gene="trxA"
                     /locus_tag="WNA25_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFA9823261.1"
                     /translation="MSSELIVHTGDAAFEQDVLKSDLPVLLDFWAPWCGPCKMIAPIL
                     DDIAAEFEGRLKVVKINIDDNEATPSRFGVRGIPTLMVFKNGEVVATKVGALAKGQLT
                     AFVEASIA"
     gene            complement(20089..21036)
                     /locus_tag="WNA25_000428"
     CDS             complement(20089..21036)
                     /locus_tag="WNA25_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFA9823262.1"
                     /translation="MTDITPFANRLGKNIKHLMKWAKRNGIEAWRIYDRDIPQFPFAA
                     DVYGDRIHLQEYDTGWLMRPGEYEAWLAEVLEAVAFVTGFEPEQIRLKRRERQKGLQQ
                     YEKTGKAGDDFVIAENGRKFWVNLDKYLDTGFFLDHRNTRKKVGETAAGKRFLNLFSY
                     TGSFTVYAATGGAASSETVDLSNTYLDWARRNFELNGIDMERHKIVRADVFQYLQTAY
                     GEGRQFDLIVMDPPSFSNSKKMPGILDIQRDHQKLIDGAVNLLASDGILYFSNNLRSF
                     VLDDSVSEQYAVKDISKQSVPEDFRNKKIHRCWEIRHKS"
     gene            21364..22752
                     /locus_tag="WNA25_000429"
     CDS             21364..22752
                     /locus_tag="WNA25_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFA9823263.1"
                     /translation="MSIKFADLNLDKNILSAVSSEGYESPTPIQAQAIPFALEGCDIM
                     ASAQTGSGKTAAFLLPTLQRLTKRSEKPGKGPRALVLAPTRELAAQVEKNALAYAKNM
                     RWFRTVSIVGGTSFGYQTRALSKPVDLIVATPGRLTDLMQSGKVDFERLEVLILDEAD
                     RMLDMGFIDDIETIVEATPADRQTLLFSATWDGAVGKLARKLTKDSEIIEVERVDGQG
                     KIEEQLLYCDDMRHKNRLLDHILRDANIDQCVIFTSTKAMTEVIADELYEKGFAANCL
                     HGDMPQGWRNRTLTDLRKGRCKILVATDVAARGIDVPTITHVINYDLPKQAEDYVHRI
                     GRTGRAGRTGIAITFAEVNEYVKVHKIEKYINRKLPELTIEGMEPARKRKSAGGKPKG
                     KGGWGDRKSGGRRGDHKPGKEGFGGKTRGEGFKKEGFKKDSFKKTGEGFKGKRKAGDS
                     FAGKSERRYKDR"
     gene            complement(22866..22972)
                     /locus_tag="WNA25_000430"
                     /pseudo
     CDS             complement(22866..22972)
                     /locus_tag="WNA25_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(23103..23303)
                     /locus_tag="WNA25_000431"
     CDS             complement(23103..23303)
                     /locus_tag="WNA25_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009348581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823264.1"
                     /translation="MPSEGLSDGIFVFVRGIVMLPFDFQTAFCFQAFDVGMAILIYVR
                     WQGGIVIPPLGFQTASYRTVKP"
     gene            complement(23317..23871)
                     /locus_tag="WNA25_000432"
     CDS             complement(23317..23871)
                     /locus_tag="WNA25_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823265.1"
                     /translation="MKKIIASALIATFALTACQDDTQARLERQQKQIEALQQQLAQQA
                     DDTVYQLTPEAVKDTIPAQAQANGNNGQPVTGKDGQQYIYDQSTGSWLLQSLIGAAAG
                     AFIGNALANKFTRAGNQDSPVARRARAAYHQSARPNARTSRDLNTRSLRAKQQAAQAQ
                     RYRPTTRPPVNYRRPAMRGFGRRR"
     gene            complement(23956..24456)
                     /locus_tag="WNA25_000433"
     CDS             complement(23956..24456)
                     /locus_tag="WNA25_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823266.1"
                     /translation="MDAMPQTAVQNENLHTCSIIVDDAQDFSPMLLDAGRDDTLISES
                     MIPQIRTVAALIAAERHDFSRSSPAEFTDAADFFAARILVLGVRRFHLDVSLLQILKT
                     ANKRACRFAEKHRLFFTPAQAELSLNKHKNRRLLTIETEHEVENKGNPVANSLAFVRK
                     LHTLPL"
     gene            24859..26052
                     /locus_tag="WNA25_000434"
     CDS             24859..26052
                     /locus_tag="WNA25_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HFA9823267.1"
                     /translation="MQNYLTPNFAFAPMIPERASGSRVWDTEGREYIDFSGGIAVNAL
                     GHCHPALVDALNAQMHKLWHISNIYTTRPAQELAQKLVKHSFADKVFFCNSGAEANEA
                     ALKLARKYARDRFGGGKSEIVACINSFHGRTLFTVSVGGQPKYSKDYAPLPQGITHVP
                     FNDIAALEAAVGEQTCAVIIEPIQGESGILPATAEYLQAARRLCDRHNALLILDEVQT
                     GMGHTGRLFAYEHYGVVPDILSSAKALGCGFPIGAMLATEKIAAAFQPGTHGSTFGGN
                     PMACAVGSRAFDIINAPETLHNVRSQGQKLQTALLDLGRKTGLFSQVRGMGLLLGCAL
                     DTPYRGRSSEIAATSLKHGVMILVAGADVLRFAPSLLLNDEDIAEGLRRLEHVLTEFA
                     AANRP"
     gene            complement(26159..27403)
                     /gene="clpX"
                     /locus_tag="WNA25_000435"
     CDS             complement(26159..27403)
                     /gene="clpX"
                     /locus_tag="WNA25_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFA9823268.1"
                     /translation="MSNENRTCSFCGKSKSHAKHLIEGKNAYICDECVANCLEILYEG
                     DNGGTPPENAGGEPEESGKLPTPAEIVANLDDYVIGQGQAKKALAVAVYNHYKRLRHP
                     KADGGVELSKSNILLIGPTGSGKTLLAQSLARKLDVPFVMADATTLTEAGYVGEDVEQ
                     IITKLLGKCDFDVEKAQHGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VASVPPQGGRKHPNQEFINVDTANILFICGGAFAGLEKVIRQRTEKGGIGFGASVHSK
                     DENAGITKLFGIVEPEDLIKFGLIPELIGRLPVIATLEELDEDALINILTEPKNALVK
                     QYQALFGIENVGLEFEEGALRSIARQAMERKTGARGLRSIVERCLLDTMYRLPDLQGL
                     KKVVVGKAVIEEGREPELVFES"
     gene            27586..27957
                     /gene="rbfA"
                     /locus_tag="WNA25_000436"
     CDS             27586..27957
                     /gene="rbfA"
                     /locus_tag="WNA25_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFA9823269.1"
                     /translation="MRKPQRGYARQDRVKEQIMRELAELVRTGLKDPRAGFITINEVE
                     VTRDYSHATVFYTVLNQDTREITEEVLEHARGHLRSELSKRIKLFKIPELHFKYDESL
                     ERGMSLSALIDQVAAEKPVED"
     gene            27968..28492
                     /locus_tag="WNA25_000437"
     CDS             27968..28492
                     /locus_tag="WNA25_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823270.1"
                     /translation="MNNLIPEHLAAYAHSDNLQIEGGHRCFSLSCQGRDTFHIRYYGE
                     PFDGLITDTDKAPVKIVAVEAVSGDEIVLFDGAEHGYNAMFCDKYSQNQKQNRTLTDL
                     DEYTYRVPIHLYYNIDYEDEYEDFVNSEGQVPLIDGRIISFDSLKRNGFDAISIDLID
                     EKHSVRELLNEELS"
     gene            28550..29470
                     /gene="truB"
                     /locus_tag="WNA25_000438"
     CDS             28550..29470
                     /gene="truB"
                     /locus_tag="WNA25_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFA9823271.1"
                     /translation="MTNKPAKRPVNGVLLLDKPEGLSSNTALQKARRLFHAEKAGHTG
                     VLDPLATGLLPVCFGEAAKFAQYLIDADKAYTATLKLGEASSTGDAEGEIIAAARADI
                     SLAEFQTACQALTGNIRQVPPMFSALKHEGKPLYEYARKGIVIERKPRDITVYSIDIA
                     EFDAPKAVISVRCSKGTYIRTLSEGIAKHIGTFAHLTALRRTETAGFTIAQSHTLEAL
                     ANLNETERDGLLLPCDVLVSHFPQTVLNDYAVHMLQCGQRPRFEEDLPSDTPVRVYTE
                     NGRFVGLAEYQKEICRMKALRLMNTAASSA"
     gene            29688..30188
                     /locus_tag="WNA25_000439"
     CDS             29688..30188
                     /locus_tag="WNA25_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823272.1"
                     /translation="MKTGIQTELAQALLSHEKIWANEEKTILAKNILLDLVEKTDPSI
                     IGLLLNNDELKRHFFVEVNGVLVFKLQDFRFFLDKHSINNSYTKYANRIGLTDGNRFL
                     KDNSDIVLDFPFKDCVLNGGQSTEEGEEIYFKRNNSQPASQPASQPASQPIIHQINQK
                     KTRNLF"
     gene            30394..31854
                     /locus_tag="WNA25_000440"
     CDS             30394..31854
                     /locus_tag="WNA25_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006996595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFA9823273.1"
                     /translation="MIYIDPPYNTETDSFAYNDKFSHSTWLTFMKNRLEIAKELLKDD
                     GLIFVQCDDKEQAYLKVLLDETFTRENFINCIAVKMSEPSGNKMAHTSHRLPKIKEYI
                     LIYKNKNIKLNPIREQKSEWDNEYNIFLENFTQEDKKFIDLIVNSQTENKEINGNTLK
                     EIDILLKKISPISVNQKLAQLNIKDNEVIKWKLDNAYRIVRTAASSSVKKLADEKKEI
                     CQQQFFSVISKRDKLLYIVKSDYSKDAKAPRVQVLFAEDYLSISLCDLWTNINTTGLE
                     AEGNVELKNGKKPESLIETIIKLATNENDIVLDYHLGSGTTAAVAHKMNRQYIGIEQM
                     DYIETLAVERMKKVIDGEQGGISKAVNWQGGGEFVYAELSPFNETAKQQILACENSDG
                     IKTLFEGLCERHFLKYNVSVNEFSQIIEEPEFQSLALDEQKQMMLEMLDLNQMYISLS
                     EMDDEQFAGCLNDDDKALSRAFYQSVKHQAEKKDGE"
     gene            31844..34618
                     /locus_tag="WNA25_000441"
     CDS             31844..34618
                     /locus_tag="WNA25_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFA9823274.1"
                     /translation="MANNKTLFDWVEDRKSMLEEMEQTDFFALPEFVSNNLKYPFFEW
                     QKSALENFVIFDRTSKLKDFPDIKNRPTHLLFNMATGAGKTMMIAALILYYFEKGYRH
                     FLFFVNKNNIVYKTENNFIDPTHPKFLFTEKILQGDTVIPIRKVETFSQYSDGIEIKF
                     TSIQKLYNDIHTERENQTTLADLHELNLVMLGDEAHHLNAQTKGKKQGELDLEKEMNE
                     RTSDAEFERKGWEHMVLELLLNKNGNPGQNVLLEFTATLPENTEVQQKYADKIITKFG
                     LKEFLQKGYTKEINLVSGTLNKKERVLHALLFAWYRHQIALKYGIANFKPVMLFRSKT
                     IDESKADYSAFLNWVENVQADDFSFLTTFSTSLNDSNNANEQGKTRTEQALKFMQENK
                     FEFVHLANWIKQNYQKHNVIITNSETNKNKTEKTDSETEKLLNNLEAADNPIRAIFTV
                     DRLTEGWDVLNLFDIVRLYEGQNGGGSNKKSGKTAAATVSEKQLIGRGVRYFPFAFEG
                     KQPNKRKFDNDMQHELRILEELFYYTHDEQSRYITELKNELRKDGYLPEKDDDKVLAT
                     FKLKSEFADNKDFRELLIWANKKIPNPNAKSNNADSLQANPQTLSFQVHGNQLLQETQ
                     FTADENDETARQIGTQNNFTQTIKMSEMERHIFNKALHIKGKNSQSLFHFDRLQSKLD
                     IQNRNELQNKLLKDWQIEFLGLEQDKQVRPDDKLAGCLKILEMVEKHLNESDIPFIGT
                     KEFTPKKLWEIFGTPKQKWVKKDDIKTAIATQNDWYVMDNFAGTGLEEALIQFISARL
                     GDLKSQYDVHLIRNEEVFKLNNFADGEGFMPDFVLLLKDKQKSSSDSVDDFLHYQIFI
                     EPKGGHLVENDSWKEAFLKSITVEYGRDKILQKNTPHYRLIGLPFFTDHQKNGQFTEL
                     FPLGAASLEK"
     gene            complement(34767..35939)
                     /locus_tag="WNA25_000442"
     CDS             complement(34767..35939)
                     /locus_tag="WNA25_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFA9823275.1"
                     /translation="MKRDLSKMTCIEDLRRVAKRKMPRMFYDYIDSGSWTETTYRENT
                     SDFKDIRFRQKVLVNMEGRSLETKMIGGDVKMPVAIAPTGFTGMAHADGEILAARAAE
                     KFGIPFTLSTMSICSIEDVAENTSAPFWFQLYVMRDREFMENLIKRAKDAKCSALVLT
                     ADLQVLGQRHKDIKNGLSAPPKPTIANLINLATKPEWCMKMLNTERRTFRNIVGHAKN
                     VGDLSSLSSWTAEQFDPRLSWDDVARIKDLWGGKLIIKGIMEPEDAEKAAKSGADALV
                     VSNHGGRQLDDTVSAIKALPDIVSAVGSDIEVWMDSGIRSGQDILKAWALGAKGTMIG
                     RAFLYGLGAYGEEGVTRALEILYKEMDVSMAFTGHRDIQDVDASILRSKDWGRETV"
     gene            complement(36060..36290)
                     /locus_tag="WNA25_000443"
     CDS             complement(36060..36290)
                     /locus_tag="WNA25_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823276.1"
                     /translation="MVVVARVGFFIVGGIVQYLSVSVKYGGAVADCLFKWRYSLILLY
                     LFGFLNQLVMFFEERRLGRFAGKRFQNDLIYF"
     gene            36289..36735
                     /gene="iscR"
                     /locus_tag="WNA25_000444"
     CDS             36289..36735
                     /gene="iscR"
                     /locus_tag="WNA25_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFA9823277.1"
                     /translation="MRLTTKGRFAVTAMLDLAMNAQTGAVKLSAISERQNISLSYLEQ
                     LFGKLRRAGLVESLRGPGGGYILAAPPAQINIAQIISAAEDRLDATQCGSKANCHHGA
                     PCLTHDLWENLNKTINDYLGSVTLQSIIEQKNNGDGSRVVQFTHIH"
     gene            36764..37978
                     /locus_tag="WNA25_000445"
     CDS             36764..37978
                     /locus_tag="WNA25_000445"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HFA9823278.1"
                     /translation="MTVKTPVYLDYAATTPVDKRVAEKMIPYLTETFGNPASNSHAFG
                     WTAEEAVEKARADIAALINADPKEIVFTSGATESDNLAIKGAANFYKTKGKHLITVKT
                     EHKAVLDTMRELERQGFEVTYLGVQENGLIDLEELKAAIRDDTILISIMWVNNEIGVV
                     QNIPAIGEICRERKIAFHVDAAQACGKVPVDVEAAKIDLLSMSAHKVYGPKGIGALYV
                     RRKPRVRLEAQMHGGGHERGFRSGTLPTHQIVGMGEAFRIAKEELAQDTAHYLKLRDI
                     FLKGIEGIEEVYINGDLEHRAPNNLNVSFNFVEGESLIMAVKELAVSSGSACTSASLE
                     PSYVLRALGRNDELAHSSLRITFGRMTTEEEVQFAAELIKSKIGKLRELSPLWEMFKD
                     GIDLNSIEWAAH"
     gene            37963..38274
                     /locus_tag="WNA25_000446"
     CDS             37963..38274
                     /locus_tag="WNA25_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823279.1"
                     /translation="MGGALRLMSNKGCLRKNTMKVKIINLPNGYKRIIYGKYFEQFDL
                     DYEQDLDVLKKDIEFALSVIEYNRSIFKKFSSLFENKIIFVYQGGHHLDIIDRDKGSL
                     K"
     gene            38607..39074
                     /locus_tag="WNA25_000447"
     CDS             38607..39074
                     /locus_tag="WNA25_000447"
                     /inference="COORDINATES: protein motif:HMM:NF014691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFA9823280.1"
                     /translation="MAHLQTIHRELFGNVYDWAGKIRRVDISKGNTRFANFAFIENES
                     RKLLEKLKNENYLRVLDKDKFAERAAYYLDELNVLHPFREGNGRTLRLFMTQLAIKNG
                     FQIHWQNISAEQMIQACIQAYHADSSLLARLIIRQFRTIVFFRRLKHQQTTIF"
     gene            39087..39473
                     /gene="iscU"
                     /locus_tag="WNA25_000448"
     CDS             39087..39473
                     /gene="iscU"
                     /locus_tag="WNA25_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFA9823281.1"
                     /translation="MAYSDKVIDHYENPRNVGTFDKNDESVGTGMVGAPACGDVMRLQ
                     IKVNDEGIIEDAKFKTYGCGSAIASSSLITEWVKGKSLDDALAIKNSEIAEELELPPV
                     KIHCSILAEDAVKAAVADYRKRQENK"
     gene            39560..39880
                     /gene="iscA"
                     /locus_tag="WNA25_000449"
     CDS             39560..39880
                     /gene="iscA"
                     /locus_tag="WNA25_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFA9823282.1"
                     /translation="MITLTENAAKHINDYLAKRGKGLGVRLGVKTSGCSGMAYNLEFV
                     DEANGDDLIFEGHGARIYIDPKSLVYLDGTQVDYTKEDLQEGFKFENPNVKDSCGCGE
                     SFHV"
     gene            complement(39840..40061)
                     /locus_tag="WNA25_000450"
     CDS             complement(39840..40061)
                     /locus_tag="WNA25_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor A"
                     /protein_id="HFA9823283.1"
                     /translation="MGGKAQHNKGKIRDNALKALVKSDLFRHKVERKRKGKGSYNRQE
                     AKKRRDGFDTVPPFLCLKRGSSRRSRRSL"
     gene            40143..40643
                     /gene="hscB"
                     /locus_tag="WNA25_000451"
     CDS             40143..40643
                     /gene="hscB"
                     /locus_tag="WNA25_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HFA9823284.1"
                     /translation="MSQYFTLFRIEPAFDIGTENLEQTYRALAARFHPDKFASASAFE
                     QKQAVMMSSTINDAYRTLKNPIDRAAYLLKTSGIDADAPEHTSFAPDFLMQQMEWRET
                     LMEARAGNNLESLKNLDNEIRAEQEKLFCGLKQSFARQDCDTAAQQVRQGRFLDKLRH
                     EISSAL"
     gene            40741..43491
                     /gene="gyrA"
                     /locus_tag="WNA25_000452"
     CDS             40741..43491
                     /gene="gyrA"
                     /locus_tag="WNA25_000452"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFA9823285.1"
                     /translation="MTDATIRHDHKFALETLPVSLEDEMRKSYLDYAMSVIVGRALPD
                     VRDGLKPVHRRVLYAMHELKNNWNAAYKKSARIVGDVIGKYHPHGDFAVYATIVRMAQ
                     NFAMRYVLIDGQGNFGSVDGLAAAAMRYTEIRMAKISHEMLADIEEETVNFGPNYDGS
                     EHEPLVLPTRFPTLLVNGSSGIAVGMATNIPPHNLTDTINACLRLLDEPKTEIDELID
                     IIQAPDFPTGATIYGLGGVREGYKTGRGRVVMRGKTHIEPIGKNGEREAIVIDEIPYQ
                     VNKAKLVEKIGDLVREKTLEGISELRDESDKSGMRVVIELKRNENAEVVLNQLYKLTP
                     LQDSFGINMVVLVDGQPRLLNLKQILSEFLRHRREVVTRRTLFRLKKARHEGHIAEGK
                     AVALSNIDEIIKLIKESPNAAEAKEKLLARPWRSSLVEEMLTRSGLDLEMMRPEGLAA
                     NIGLKKQGYYLSEIQADAILRMSLRNLTGLDQKEIIESYKNLMGKIIDFVDILSKPER
                     ITQIIRDELEEIKTNYGDERRSEINPFGGDIADEDLIPQREMVVTLTHGGYIKTQPTT
                     DYQAQRRGGRGKQAAATKDEDFIETLFVANTHDYLMCFTNLGKCHWIKVYKLPEGGRN
                     SRGRPINNVIQLEEGEKVSAILAVREFPEDQYVFFATAQGMVKKVQLSAFKNVRAQGI
                     KAIALKEGDYLVGAAQTGGADDIMLFSNLGKAIRFNEYWEKSGNDEAEDADIETEISD
                     DLEDETADNENTLPSGKNGVRPSGRGSGGLRGMRLPADGKIVSLITFAPETEESGLQV
                     LTATANGYGKRTPIADYSRKNKGGQGSIAINTGERNGDLVAATLVGETDDLMLITSGG
                     VLIRTKVEQIRETGRAAAGVKLINLDEGETLVSLERVAEDESELSGASVISNVTEPEA
                     EN"
BASE COUNT        11626 a        11953 c        10594 g         9519 t
ORIGIN      
        1 gtacagaccc ttgtttttta ttccccggtg tctgttttct gccacagtcg atttagattc
       61 cagcaaaccg cctttaaaca attgaaagaa aagggacaaa ttgcacaggg ttctccgttt
      121 aaccagatac ggatttccat tcccggcaat ttcgggcatt tgaccgtttc cgccttcctg
      181 ccccgccgcc aataaaaatg ccgtctgaaa tatcggcacg atacttcaga cggcataccc
      241 gcccgttttc ccgcatttcc gggagctggc tgaaatttaa acgtgtgcgg aaatgatttt
      301 taacatttgc gccagtactt tgggattggc agccacaata tcgccgcttt ccaaccaagc
      361 gtcctcaccc gacatatcgg taacgatgcc gcctgcttct tggacaatca atgcaccggc
      421 ggcaatgtcc cacggtttga ggttgaactc gaaaaagccg tcaaaacgtc ctgttgctac
      481 ggcgcacaaa tccaaagaag ccgcgccttc gcggcggcct ccggcggttt ttgccaaaaa
      541 gtctttcaaa atcgccagat atttgtccat catgctttga tcgacaacag ggaaaccggt
      601 accgatcagg cagcggttca attcgatgcg gttggagaca cggatgcggc ggtcgttaag
      661 caacgcgcct ttaccgcgcg aagccatgta cacgtcgtta cgttcggggg agtacaccaa
      721 agcttcctgc aacacgcctt tgtgcagcag tgccatagag atggcgtatt ggggatggcc
      781 gtgaaggaaa ttggtcgtcc cgtcgagcgg atcgataatc cattcgtact cggctgtagc
      841 tttgccgtgg aagccgcttt cttcgcaagt gattttgtga tgcggatagg cttctttcaa
      901 agcctcaacc aggatgattt cggaattgcg gtcaacatcg gaaacaaaat cgttgaaggc
      961 tttgctgtcg gttttgacgg catcgaggtt acctgcggcg cgaatcatca tctgaccggc
     1021 acggcgggcg gctttaaagg ctgtattcaa aaacggattc atcagatttc cttaagggtg
     1081 gcataccgcc ggttcggacg tgcagtcctt cggagcggca aaatcggagt ttatctggtt
     1141 ggggcaaacc ctgccaaatc gggtaaaata ccgcctgacg cgtgtctgct tcaggcgcaa
     1201 cgttaaattt ccgacgttgt taaagaacag ttcaaacggc atttcaccgt ccgaacgaaa
     1261 aagacggcgc attataccct attccattcc gaccgaaaac cgaacatgac tgctctcaaa
     1321 cccgccctgc ccgactacct cggcaacatc cgcatcatcc tcacacgcac cagccacccc
     1381 gccaacatcg gctctgccgc acgcgcgatg aaaacgatgg gtctgcacag gctgaccatc
     1441 gtcaccccca atctgatggc aacgccgatg acggaaaatc cgcccgtttt taatcccgat
     1501 gacgtgcaaa gttttgcact gcctgaagaa agtttcatcc tcgcttccgg cgcggcggac
     1561 gtattgcaca acgccgaaat cgtcgccaca ctggacgaag cccttgccga caccaccatc
     1621 gcctgcgccc tgaccagccg ccgccgcgaa attaccgcgc cgctgcaaac cccgcgcgat
     1681 ttggtgcccg aattactgca ggccgccaac cgcggcgaga aagtggcgct ggtcttcggc
     1741 aacgagactt tcggcttgag catcgaagaa gtccgagcgt gcaaccgact gatgaccatc
     1801 aacggcaacc ccgactattt ctcgctcaac ctcgcccaag ccgtgcaggt cgtgtgctac
     1861 gaaattttca gccaaaccga ttcgcccatg acccatcttc aacaggaaga ccacgccgca
     1921 acccacgagc aaatcaaagg catgctcgcc cacatggaaa gcgtgatgga cgacatcggc
     1981 tttttcaacc gccgcaacgg cgagcgtctg atgcgccgta tgcagagcct gttcggacgc
     2041 gccaacacgc aaaccgaaga catcgacatc ctgcgcggtt ttttcaatac cgtcagccac
     2101 cgtatccata aaaaagactg attaaggccg tctgaaaaca ttacaagctt tttcagacgg
     2161 catgactgat attcggataa gcatgaatta cgccctagac gcattatggt ggaaactcac
     2221 cagccaaccc gtccgcgacc tcgcctcgct gctgactgcg ccgcctttgt ggcaaagcgg
     2281 ttgcgaattg agcgtgcgcg aactactggg ggaacgcggt ttccgctacc ttttagcatt
     2341 ggatgccgat cccgcgccgc tgacggatta ccttgcacaa cgcgccccgt tcgaccaccg
     2401 tctcggcatt tatgccgaag agctgctggc tttttggttt accaacgcac cgcacaccaa
     2461 actgcacgca tataatctga ccgttttttc agacggccaa actttaggcg ccgcggattt
     2521 tgtcgtttcc ctcaaccaac agccctatca tatcgagctg gcgtgtaaat actacggcgg
     2581 agaccaagtg cagaacctgc gcggtctcaa tcccaaagac acgctgacgg acaaagccgc
     2641 caaattggtg cagcaattcc agctggtgca tacgtcgcaa ggcaaagcaa ccttagccgc
     2701 acaagacctg ccggaaaatc cacttcctgc ttccatcgtg cgcggcatcg gattttttcc
     2761 acaaggtttc catgcttttg agccgccgct taatccatac ggctggcgcg gcatctatat
     2821 tcaagattgg gcggaatacg ggtttgaacg ccaagaagcg cgctatcacc tgctcgaccg
     2881 catggtctat ctcgcgcctg cgcgtgtcgc cgaaaccgaa acattaaacg caaccgaaat
     2941 ccgccgcatc gaccaaggct tgattgccgt tttggaatgt cggccggacg gcttttggca
     3001 cgaaatcgaa cgtattatga aggccgtctg aaacccttta ccaactttaa gagaacccat
     3061 tacattatga acgccgcaca actcgaccat accgccaaag ttttggctga aatgctgact
     3121 ttcaaacagc ctgccgatgc cgtcctctcc gcctatttcc gcaaacacaa aaagctcggc
     3181 cgccaagacc gccacgaaat cgccgaaacc gcctttgccg cgttgcgaca ctatcaaaaa
     3241 atcagtaccg ccctgcgccg tccgcacgcg cagccgcgca aagccgcact cgccgcgctg
     3301 gttctcggca gaagtaccaa catcagccaa atcaaagacc tgcttgatga agaagaaaca
     3361 gagtttttcg gcaatttgaa agcccgtaaa accgagtttt cagacggcct gaataccgcc
     3421 gcagaattgc cgcaatggct ggtggaacaa ctgcaacagc attggagcga agaagaaatc
     3481 ctcgctttcg gccgcagcat caaccaagct gcgccgctcg acatccgcgt caataccttg
     3541 aaaggcaaac gcgacaaagt attgccattg ttgcaagccg aaagccccga tgcagaggca
     3601 acgccttatt ctccttgggg catccgcctg aaaaacaaaa tcgcgcttaa caaacacgaa
     3661 ctgtttttgg acggcacgct ggaagtccaa ggcgaaggca gccagctgct tgccttattg
     3721 gtgggcgcaa aacgcggcga aatcattgtg gatttctgtg ccggagccgg cggtaaaacc
     3781 ttggctgtcg gcgcccaaat ggccaacaaa ggcagaatct acgccttcga catcgccgaa
     3841 aaacgccttg ccaacctcaa accgcgcatg acccgtgccg gactgaccaa catccacccc
     3901 gaacgtatcg gcagcgaaca cgatacccgt atcgcacgac tggcaggcaa agccgaccgc
     3961 gtgttggtcg atgcgccctg ctccggctta ggcacactgc gccgcaatcc cgacctcaaa
     4021 taccgccaat ccgccgagac cgtcgccaaa cttttggaac agcaacacag tatcctcgat
     4081 gccgcctcca aactggtgaa accgcaaggc tgtttggttt acgccacctg cagcgtgttg
     4141 ccggaagaaa acgaactgca aatcaaacgt ttcctgtccg aacatcccga atttgaaccc
     4201 gtcaactgcg ccgaactgct gcaaaacctg aaggtcgatt tggataccgg caaatacctg
     4261 cgcctcgatt ccgcccgaca ccaaaccgac ggcttcttcg ccgccgtatt gcaacgcaaa
     4321 taaaccggtt tgaacaaaat gccgtcagaa cccttttcaa agcgttcgga cggcatttca
     4381 tcaattaaaa atatcaaaaa ccgcagattc aaccgattca acaccaaagg cagaatcacg
     4441 cgcctcatcg gattttatac gcgcctgcct gcaattttct ttttctttgc caaaatatac
     4501 ggtagcataa ccatattcca atgatatttt tgggaatctg ttttacccca atatataaag
     4561 caccatatta aggcggagtg tcttccctgc tttgacccga acccggaaaa gacaccgccc
     4621 gaaccaatcc cgattctgcc cgggcagtca gccatcaagg aaaccctaat gaactttgct
     4681 ttatccgtca tcgcatttac cctcgcctct ttcctgcccg tcccgcctgc cggagccgcc
     4741 gtctttactt ggaaagacgg cggcggcaac agctattcgg atgtgccgaa acagcttcat
     4801 cccgaccaga gccaaatcct caacctgcgg acgctccaaa ccaaaccggc ggtcaagccc
     4861 aaacctgccg tcgatacgaa tgcggacagt gcgaaggaaa acgaaaagga tatcgccgag
     4921 aaaaacgggc agcttgagga agaaaagaaa aaaattgccg aaaccgaacg gcagaacaaa
     4981 gaagaaaact gccggatttc aaaaatgaac ctgaaggcgg tgggaaactc aaatgcgaaa
     5041 aacaaggatg atttgatccg taaatacaat aacgccgtaa acaaatactg ccgttaatcg
     5101 gctctagcgc aaacccgatg ccgcctgaag cggcacgggg tttgtttatt tctgccagta
     5161 ggttttgacg ttgacgaact cgtacaaccc gaattcggac agttcgcgcc cgtaaccgga
     5221 atctttgact ccgccgaaag gcaggcgcaa atcgctgctg gtatggcggt tgataaacac
     5281 cgatccggcc tgtatttttt cggcaaaccg ccaggcgcgt tcagtatcgg cggtataaat
     5341 gcaggcaccg agcccgaacg gggaatcatt ggcaaggcag atggcgtgtt cttcgttttc
     5401 ggcgcgcaaa atcagggcga cggggccgaa tacttcttct ctccagacgc ggcaggcagg
     5461 atttaccctg tctaaaaccg tcgcagggta aaaccacccc gacccctgtg gaactttgcc
     5521 tccggtcagg cacaccgcgc cgtttgaaac ggcatcttca acctgcccgt gaacccggtc
     5581 ccgcaaatct tcgcggtgca gcggtgcaag cgtggtatcg ggatgtttcg ggtcgcccat
     5641 tttcaatttg gcgcattcgg cgagaaacag ctgaataaag cggtcggctg cggcttcggt
     5701 tacgatgatg cgcttggcgg cgttacacga ttgccccgca tcgcggaaac gggaataaca
     5761 ggcttctgcg gcggcgcgtt ccaaatcggc atcgggcatc acgataaagg cgttgctgcc
     5821 gccgagctcc aacacggttt tcttgaggtt tgcgcccgca tgtgccgcaa gaatgcgccc
     5881 cgtatgcgtc gaaccggtaa acgccatcgc atcggtatct tcgactgcct tgagcgtgtc
     5941 cgcctcatcc agccacgcgc ctgccagagg aataccgtct gaagccaaat cgaacaacgc
     6001 ccgactgacg cgtgccacgc tgggcgcggg tttgacggcg cacgcgttgc ccgcgcacat
     6061 agcgggaacg gcaaaacgca atacctgcca gacgggatag ttccaaggca tgacggcaaa
     6121 caccacgccc aaaggctcga agcgcacctg actcaaactc gcctgcgtcg caatggtttt
     6181 gtgggcgagc agttcggggg cgaggcgggc gtaatagcgt atcagttcga tagacttgac
     6241 gatttcggca cggcattcgt gcaggcagcg tccgacttct tcgcacacca tttccgcaaa
     6301 acgctctttt tccgcctcca aacggccggc aaatttttgc agacgcgcgg cacgttcggt
     6361 tacgcccaat tgcgcgaacg ccctgccgtg cattttcaaa tccgccagcc gccgttcaaa
     6421 ctccgcataa tcttgatcgg ggcggcggta aagcgtttcg cccgtaaata cattgacact
     6481 gtgaaacatc gaatcaacct gccagttgcg ggaatatcgt tttcagtccc gacacaataa
     6541 tctccaccga taccgccgcc agcatcatac ccataatgcg gtttaaaatc gtcagccccg
     6601 tcgcgcccag caggcggctg accttcccgg caacgattaa aatggcataa caaatcgcac
     6661 tgaccaccaa accggccgcg ataatcagcg cgatatcgct gtacgttttg gctgccgaag
     6721 cgtaaataat cacggtcgaa ataccgcccg gaccgatggt gatcggtatg gcgatgggca
     6781 cgacggcaat cgcccctgca ttgcgggcgg ggcgcgcttg ccccgtttcc ggctgcgcgc
     6841 cgagattctg cttggcggga ttgtcgttgc cgttcatcat cgaaatggcg atcagcagca
     6901 ccaaaatccc gccgccgacc tgaaacgaac cgacgctgat gcccaaaacc ttcaatagcg
     6961 caccgccgat cagcgcaaat accgcaatca cggcaaacac ggcgacggcg gccgtccgcg
     7021 cgaccttcct gcgctccttc gtgctgtgtc cgttggtcag gtcaaggtaa agcgacaacg
     7081 cgctaaacgg attgatcagc accaaaaaag ccacaatcag cttgccgatt tccataccca
     7141 atcccattat ttcccctctt tcaaacccgt gcggcaggca tccgatgctg caaattgccg
     7201 ccgcaacgga tttttccgtt ataattaaaa atccaagcaa tacgccccat catacccgaa
     7261 cgacggtatc tttaccatca gacaaggatg cttttcatgg cactgacact tgccgacgta
     7321 gacaaaatcg cccgactctc ccgactgcag ctgactgcgg cagaaaaaga aaaatcgctt
     7381 caagaattaa acgacatttt cgctatggtc gaacagatgc aaaccattaa cacagacggc
     7441 atcgaaccga tgactcaccc gcacgaggcc gccctgcgcc tgcgcgaaga cgaagtaacc
     7501 gaaaccgacc gcgccgtcga atatcaggcg gtcgcaccgg aagtacgcaa ccgtctgtac
     7561 atcgtaccgc aagttatcga agaataatcc gaatatgctt cagacggcat cagcaatacc
     7621 gcctgaaacc ctttagggat ggaagattta tgacacaata cacattgaaa caggcaagcg
     7681 tcctgttgca gtccaaacag atttccgccg tcgaactagc aagcgcatac cttgccgcca
     7741 tcgccgaaaa aaatcctgcc ctcaacggct acatcaccat cgaccaagat aaaaccctcg
     7801 cagaagcccg tgccgccgat gaacgcatcg cgcagggcaa cgcctccgcg cttaccggcg
     7861 ttcccgtcgc ctacaaggat attttctgcc aaaccggctg gcgcagcgcg tgcagctcca
     7921 aaatgctgga caacttcgtc tccccctaca cagccaccgt cgtccaaaac ctgctcgacg
     7981 aaggcatggt aacgctcggc cgcaccaata tggacgagtt cgctatgggt tcgaccaatg
     8041 aaaactcatt ctacggcgca gccaaaaacc cgtggaatct tgagcacgta cccggcggtt
     8101 cgtccggcgg ttccgccgcc gtcgttgccg cgcgcctcgc ccctgccgcg ctcggttcgg
     8161 ataccggcgg ctctatccgc caacccgcgt cgcactgcgg cattaccggc atcaaaccta
     8221 cctacggcac ggtttcccgc ttcggcatgg tcgcctacgc ctccagcttc gaccaggccg
     8281 gcccgatggc gcaaactgcc gaagactgcg cgattctgtt aaacgcgatg gcaggtttcg
     8341 accccaaaga ctccaccagc ctcgagcgcg aaaaagaaga ctacacccgc gatttgaacc
     8401 aaccgcttaa aggcatgaaa atcggcctgc ccaaagaata cttcagcgaa ggcaacagca
     8461 ccgatgttca gacggcattg caacatacca ttgacctcct caaagcccaa ggcgcggaat
     8521 tggtcgaagt ttccctgccg caaaccaagc tgtccatccc cgcctactac gtcctcgcct
     8581 ccgcagaagc cagtaccaac ctttcacgtt acgacggcgt acgttacgga caccgtgccg
     8641 cccaattcgg cgatttggaa gaaatgtacg gcaaaacccg cgccgaaggt ttcggcagcg
     8701 aagtcaaacg ccgcatcatg atcggcactt acgtactatc gcacggctac tacgatgcct
     8761 actatctcaa agcccaaaaa ctgcgccgcc tcgttgccga tgactttcag acggcatttg
     8821 cacggtgcga cctcatcctc gcgccgaccg caccctccgc tgcacccaaa atcggagcgg
     8881 atgcttcgcc ggttgaaacc tacttgagcg acatttacac catcgccgtc aacctcgccg
     8941 gactgcccgc attgaccctg cccgcaggct tcagcgacgg cggactaccc atcggtgtac
     9001 agcttatcgg caactacttc gccgaagcca aaatcctcgg tgcggcgcat caaatccaac
     9061 tcaatagcga ttggcacggc aaacgacccg aatgaagcag aaccgcacct ttaccttccc
     9121 cgattttcgc accgtttaca gctatgcccc tttatatcgg ctgcaacatt taaaatacac
     9181 attgcgaaaa tttttcggaa aaaaagaaat ttacgccttc gagcagtttg tcaacgcctc
     9241 ccctatccgt caggggctgt tcctccactg cccgcaggat gcctatccgc tgctgcgcga
     9301 atttgttgac aggcgtttta actgcaaacg ccgtttagat gcgatgacgg cagattttct
     9361 tatggcggaa aaactgttcg gcacagacat tctgcaccaa atggaagact accgcttcca
     9421 tctggtcttg gcgcaccttt cagacggcat cagcttgtgg ctcaaccgca acgacaactg
     9481 cgtcgaagaa ggcgcgtggt ctttatcttt gcgcgatgaa gcaggcaacc ggctgtatat
     9541 ggcgaccttc gcctttgtcg gcacacacct gctggcagcc tccgtacaag ggccttcggg
     9601 cgaagaggcc aaagacaccg tccgccgcat caccaaacaa cttcacggcc tgcgtcccca
     9661 acaactgatg gtaaccgcac tgcaatattt cgccgccgca ttaaaattag acggcgcgat
     9721 aggcattgca caaaaacatc aggtcaaact gcgttggaaa cttaaaaagc gcgtcaaaat
     9781 gaattacgac acattctggc aggaatacgg cgcaagtttg gaacgggacg gctactggca
     9841 tctccctcaa actcccgccc gcaaagacct tgccgacatc gaaagcaaaa aacgctcgat
     9901 gtaccgcaag cgttatgaaa tgctggacaa catggttgca gagatgaagg acagtctgaa
     9961 aacagaagca cgcggcattt cagacggcat ccaaacggaa aaaccgctcc gccggacagc
    10021 ctgacgagaa gactatcgaa ttgatatttt agagaaagaa cctcctatga cctgggaaac
    10081 cgtaatcggc ttggaaatcc acgtccaatt gaacaccaaa tccaaaatct tcagcggcgc
    10141 atcggccgca ttcggctcag aacccaacgc gcacgccagc gtagtggagt gcgcgctgcc
    10201 gggcgtactg ccggtaatga accgggaagt cgttgaaaaa gccatcaaat tgggtttggc
    10261 cttagatgcg aaaatcaatc agaaaaacgt gttcgaccgc aaaaactact tctatcccga
    10321 cttgcccaaa ggctatcaaa tcagtcagtt ggacttaccg attgtcgaac acggcaaatt
    10381 ggaaatcgta gtcggcgacg atgtgaaaac catcaacgta acccgtgccc atatggaaga
    10441 agacgcaggc aagtccgtgc atgaaggctt gaacggcgcg accggtatcg acctgaaccg
    10501 cgccggcacg ccgctgttgg aagtggtatc cgaacctgaa atgcgctccg ccgccgaagc
    10561 cgttgcctac gccaaagcct tacacagctt ggtaacctgg ctagacattt gcgacggcaa
    10621 tatggcggaa ggttcattcc gcgtcgatgc caacgtatcc gtgcgcccga aaggtcaaga
    10681 agatttcggc acgcgccgcg agattaaaaa cctcaattcc ttccgtttct tggatcaggc
    10741 gattaattac gaagccgaag cgcaaatcga gattttggaa gacggcggca cagttcagca
    10801 ggcgacgatg ctgttcgacc cggaaaaagg cgaaacccgc gtgatgcgcc tgaaagaaga
    10861 cgcgcacgac tacggctact tccccgatcc cgatttgctg cccgtcatca tttcagatgc
    10921 ccaaatgcaa aaagccaaag cagaaatgcc cgagctgccg aaagaaatgg cggcgcgttt
    10981 cgtggcggat tacggcgtgt ccgaatacga cgcgcgcctg ctcaccgcca gccgcgtgca
    11041 ggctgcctat tttgaagaag ccgccaaaga gagcggacaa ggcaagccga ctgccaactg
    11101 gatgaacggc gaacttgccg ctacgctgaa caaagaaggc atggaacttg ccgacagccc
    11161 gattaccgcc ccacgccttg ccgcgctggt gggcaaaatt gccgatggca cattaagcgg
    11221 caaactggcg aaaaaagcct ttgaagccat gtgggcggaa ccggaaacca gcattgccga
    11281 aatcatcgaa aaacacagtt tgcaacagat gaccgatacc ggcgcggttg aagcgatggt
    11341 ggacgaagtg ctggcaaaca acgccaaagc cgtggaacag tttaaatccg gcaacgaaaa
    11401 agccctgaat gcgattgtgg ggcaagtgat gaagaccagc aaaggcaaag ccaatcccgc
    11461 gcaggtgcag gagttgatta aagccaaact tgcctgataa gcaagatgcc gtctgaaacc
    11521 caaatccgac gttcagacgg cattttcaca gtctgaccac attaaaacag cataaaaaac
    11581 aatattccat gaaccacacc gttaccctgc ccgaccaaac cacctttgcc gccggcgacg
    11641 gcgaaaccgt tttgtccgct gccgcccgtc aaaacctcaa tctgccccat tcctgcaaaa
    11701 acggtgtctg cggacaatgc aaagccgaac tggccagcgg cgatattcaa atgggcggac
    11761 actcggaaca ggctttatcc gaagcagaaa aagcgcaagg caagattttg atgtgccgca
    11821 ccaccgcgca aagcgatatc aacatcaaca tccccggctg caaagccgat gccctacccg
    11881 tccgcaccct gcccgcacgc atcgaaagta tggttttcaa acacgatgtc gccctcctga
    11941 aacttgccct gcccaaagcc ccgccgtttg ccttctacgc cgggcaatac attgatttac
    12001 tgctgccggg caacgtcagc cgcagctact ccatcgccaa ttcgcccgac caagaaggca
    12061 ttttggaact acacatccgc aggcgcgaaa acggtgtctg ctcggaaatg attttcggca
    12121 gcgaacccaa agtcaaagaa aaaggcatcg tccgcgttaa aggcccgctc ggttcgttta
    12181 ccttgcagga agacagcggc aaacccgtca tcctgccggc aaccgacaca ggctacgccc
    12241 ccatccgcag catcctgctc gaccttatcc gccaaaacag cagccgcgct gcccatttct
    12301 actggggcgc gcgtcatcaa gatgatttgt atgccctcga agaagcacaa gggttggcat
    12361 gccgtctgaa aaacacctgc ttcacccccg tattgtcccg tcccggagag ggctggcagg
    12421 gaagaaaagg ccacgtacaa gacatcgcgg cacaagacca ccccgacctg tcggaatacg
    12481 aagtgtttgc ctgcggctct ccggctatga ccgaacaagc aaaaaatctg tttgtgcaac
    12541 agcataagct gccggaaaac ttgtttttct ccgacgcatt cacgccgtcc gcatcataat
    12601 tcctcggtat aaagaggatt cgggctttcc gttcggaaca caaaaaactt cccgtccgta
    12661 ttttccccgt gaaaaaatgc cgtctgaaac ctgattccgg ttttcagacg gcatatgttt
    12721 tttcctgttc aaggcgacaa ccgctcgcgt atccagccgc catccagcaa acggtattgg
    12781 atgcggtcgt gcagcctgct cggcctgccc tgccaaaact caatcaaatc gggaatcaca
    12841 atatagccgc cccaatgcgg cggacgcggc acgtgcaaag gatgtttgag tccgaccgcc
    12901 gccgcctttg ccaccaatac cgccttgttc ggaatcacct cgctctgcgc gcttgcccac
    12961 gcacccaaac ggctctgata cgggcgactc tcaaaatatt cgtccgacaa cttctccgcc
    13021 agccttccga cacgcccttc cacgcgcacc tgacgctcca gctccggcca aaaaaacgtc
    13081 atcgccgcaa acggattgag ttccagcgaa cgccccttgc ggctgtggta attcgtaaag
    13141 aaaacaaaac cttctgaatt aacctccttc aaaagcacca tacggctgtt gggcctcccg
    13201 cgcccgtcga ccgccgccac attgaccgcc gtcggctcgt tgacctcggc gcgtaccgcc
    13261 tcgtccaacc accgctcgaa ctgctcgatc ggattatcgg cgcaatcggc ttccgacaat
    13321 tcccgtttgc tgtaatcttc ccgaatattg tgcaaatcca tttaccgctc ctcttatcat
    13381 ttgaaagatt ctactcccgc acgcaaaccg atttcaaccg ttgcacaaac tttgtcccca
    13441 aagccgcagc gacgatttca tccgcaaaac cgccgcatca ggtacaatat cgaaccgtcc
    13501 gaccgaggac ggcattttat caacccgtcc tgccgcacac gccgcagaag aaccgcctta
    13561 tcaggcgagt taggaaaaat gatgtccaag caacccacca gcaaacgcca atggcgcgac
    13621 ggcgcagccc cgtctgccaa gaaaaccgcc aaacctttca aaagcaaagc ccgtcccaaa
    13681 gacgaaacgc gcaaaaccgc cgcccaagct tacggacaaa aagcttcaga cggcatcaag
    13741 ctgcaaaacg cccccaaaca gcgcgccgcc aaagccaaaa aactcgtcgt ccgcaatccc
    13801 aaccaaaaaa ttatggaaca cgcgcgcgat ttgaaagaac gccgcagcga cctgtcgcgt
    13861 atggaacccg aacgcctgca aaaagtgctt gcagcctccg gcgtcggctc gcgccgcgaa
    13921 atggaagaat ggatcaacaa cggctgggta acggtcaacg gcaaaaccgc gcaactgggc
    13981 gacaaagtta cccccgacga ccacgttacc gtcaaaggca gcatcatcaa actcaaatgg
    14041 gccgaccgcc tgccgcgcat catcctgtat tacaaacaag aaggcgaaat cgtttcccgc
    14101 gacgacccgc aaggccgcgt cagcatattc gaccgcctgc cgcaggccgc cagcagccgc
    14161 tgggtcgcca tcggacgctt ggacatcaac accagcggac ttctgattct cactacctcc
    14221 ggcgaactcg tccaacgttt cgcccacccc agcttcgaag tcgaacgcga atacgccgtg
    14281 cgcgtactgg gcgggctgac taccgaacaa atgcgcagtc tcaccgaaga aggcgtgatg
    14341 ctcgaagacg gcttggcaaa agtcgaacgc atctacgaac aaggcggcga aggcgcaaac
    14401 aaatggtaca acatcgtgat taaagaaggc cgcaaccgcg aagtgcgccg catattcgaa
    14461 agccaaggcc tcacagtcag ccgcctcgtg cgcgtcgcct tcggccccat cggactgccc
    14521 aaccgcctca aacgcggaca gttctacgaa ctcaaccccg ccgaagtcgc caacatcctc
    14581 aaatgggcgg atatgctgct gccgggcgaa cgccgccgca aaaaagcctg aaagtaaaaa
    14641 tgccgtctga aacacctgct gtttcagacg gcattttatt cgggcgtttt caggagaaaa
    14701 ggtcgagtgc tttgacaaag accatcacca cgccgtaggc gagcggtatg acggccaatg
    14761 cccaacgcca ccagaccgat atgccgtcgc cggaaacttt ttcgcccaca aggtaagcgt
    14821 cggatatggc ggtttcgtcg tcggggttgc cgctgcgtgc ggcggttttg atgtcttttt
    14881 cgtggtgttt ttcgtgtacg gatttgacgg cgaggttgca caacaaaccg ataatcagca
    14941 ggcccgccat aatgtacatg gttacgctgt atgcctgtgc cgccggtatg ccgctgtcga
    15001 tttggctttg gcgtatgtaa ttgaccagta ccgggccgat gacggcggcg attgaccagg
    15061 ccagcaggat gcgcccgtga atcgcgccga cctgataggt gccgaacagg tctttcaggt
    15121 aggcgggaat ggcggcaaat ccgccgccgt acatggaaat aatcacgcaa aagccgatga
    15181 tgaacagggc tttgctgccg ccttcgccga tggagggaac ggcgaaatac agcagcgaac
    15241 cgagtacgaa gaagatggtg taggtgtttt tgcgtccgat tttgtcggaa acgctcgacc
    15301 acaaaaagcg tccgcccata ttaaacaggc tcaggaggct gacgaagcct gccgccgcac
    15361 ctgcgccgac tgccgcctgc ctgcctgcgg aggtttcgga aaagagttcc tgaatcatca
    15421 cggatgcctg gcccaatacg ccgatgccgg cggttacgtt caggcacaat acccagaaca
    15481 acagccaaaa ctgcggcgtt ttcatggctt gggacacgtt gacatgattg ctgctgaccg
    15541 gcttgttttg cgttttcggc acggtatagc cttcaggttt ccagccgtcg gcaggtacgc
    15601 ggatggtaaa cgcgccgaac atcatcagtg cgaggtaaaa caggcccaat acggcaaagg
    15661 tttcggcaac cccgaccgaa gcggcgtttg aaaaggcgtt catcagtgat acggaaagcg
    15721 gcgaggccag cattgcgccg ccgccgaaac ccataatcgc caaaccggtc gccatacccg
    15781 gcttgtcggg aaaccatttc atcagtgtgg aaaccggccc gatgtagccc aaacccaagc
    15841 ctacgccgcc gatgacgccg ttgcccaaat agagcaggaa gaggttgtgc gtacgcacgc
    15901 cgaatgcgga tacgaagaag cccaggctga aacagcaggc ggcggcaaat atggctttgc
    15961 gcggccctac ccgttccatc catgtgccga acagggcggc cgacgcgccc agcatcgcga
    16021 gtgcgatact gaaaatccaa cctacggtcg tcagcttcca atctccggcc gccgattcgg
    16081 ttatgccgat aagtttggtc agcggcgcgt tgaatacgga ataggcgtaa atctgcccga
    16141 tggcaaggtg caccgctaat gctgcgggcg gtacgagcca acggttgaaa cccggcttgg
    16201 caatgcttgc ctcacggtct aaaaacttca taacatcctc tttctgtcag ttgaaaaata
    16261 aaatttcatt tgcccaatgg aaacttatta aaaattataa aaaaatatcg ggttgggttt
    16321 ttatccgccc caagatgcgc cgtctgaaac atttcgggtg tgcggagagg tttctgtttt
    16381 ttccgacaaa ttcctgcggc tttccgcttc cggattcccg ctttttaggg actggattcc
    16441 cgttttcgcg ggaatgacgg cggaggggga gcggtttttc cgattgggtt taaatgcaat
    16501 cgaacaaatc ctgctgccct tgttctttgc ttacgcgcac gtcggtttcg ccgtcggaga
    16561 aggtaatgtg cagcttctgc ccctgcttca aaacatcggc gttacggatg acttgtccgc
    16621 gtgtattttt gacaacggag aagccgcgtt ccagaatgtg ctgcggcgaa acggcttcga
    16681 gcaatgcggc ttgggcggtc aggctttgac ggcgacgggc gagcagccgg ctgaaggcgg
    16741 cgggcaatgc cgtctgaagg cggacgatat cttgccggta aacggaaaca tcaggacggc
    16801 aatgttgcag ggcttgggtt tggcgttcaa aacgggcggt gtgggcgcgg aggttttgcg
    16861 tcatcgagta agacagcgtt tgcgccagct tgccgattga agcgcgctgc tcgtcaagtt
    16921 tttggcgcgg gtggcggatt tgccgcgcga gccagtcgag tttttggctg gcatcgaaat
    16981 agcgttgctc caaaacggtt ttcagacggc cttgggcttg gacgaggcgg tgcagtgatt
    17041 cttggcggtt ggggctgacc agttccgccg cgccggtcgg cgtgggcgcg cgcacgtcgg
    17101 caacgaaatc ggcgagcgtg aaatcggttt cgtgtcccac gccgctgacg acgggaatcg
    17161 tgcaggcttc gatggcgcgc acgaccggtt cttcgttaaa cgcccgcaag tcttcgatgc
    17221 tgccgccgcc gcgacagaca atcagcacat cgcattcggc gcgttgcgat gcggttttaa
    17281 tcgcttgggc aatctgaaac ccgctgcccg cgccctgaac ggcggccgga taaacgatga
    17341 cggggatttc gggcgcgcgg cgtttcaagg tggttacgac atcgcgcaaa gccgccgccg
    17401 ccagactggt aacgatgccg atacattgcg gacggacggg caaaggtttt ttgcgttccg
    17461 ccgcaaacgc gccttccgcc tgcaactgcg ccttcaaccg ctcgtaggct tcgtaaagct
    17521 gccccaaacc tttgagccgt acttcgttta cggtaatctg aaattcgccc cgcgcctcgt
    17581 aaatactgat tttgcccgcc acttcgatat ggtcgccttc tttcaaaggt tgcgccaaac
    17641 gcgccgccgc acccttgaac atcgcgcaac gcacctgcgc gcggctgtct ttgagtgaga
    17701 aataataatg cccgctggcg gcgcgggtca gattggacac ttcgcccgca atccacaggc
    17761 cggcaaggtg gttttccaaa atacttttgg caaatgcgtt caactcggaa acggacaaca
    17821 cgtcagaatg aaaaaaatca gacatcgaat caatcaaata ataaaaaata tgaatatgtt
    17881 ttgaagccca aggcggcatc gtgccgtcta aattgtcaac aatattataa cacgcgccat
    17941 cttgccgcca gccttttcct atatgacttt tttaagcggg gaatgggaaa aatattcatc
    18001 aacccgccct caatctattc aaattgcacc gccggcaggc tatgatgcgg atattttcga
    18061 caggaggaaa aatggatacg caggcagtta tcacacatat tgtccgatgg ttggacgaat
    18121 acgccgctca ggcaaatgca aaagggttcg tcgtaggcgt ttccggcggc atcgattccg
    18181 ccgtcgtctc cacgctcgcc gcccgcaccg gccgcaccac gctgcttctg gatatgccga
    18241 tacgccaaca ccccggccag ctcgagcggg caaggcggca catccgcaat ctgcaagggc
    18301 aatatgccaa tgtaagcgcg caaacggcca atctgaccga caccttccaa acctttgagc
    18361 aaaccgtcgg cgttcatcgg acggcatttg ccaatcagcc gctttcgctc gccaacgcca
    18421 gaagccgcct gcgtatgctg accctgtact actacgggca gatacacgga ctgctggtta
    18481 cggggacagg taataaggtt gaagatttcg gcgtgggttt ttttaccaaa tacggcgacg
    18541 gcggcgtgga catcagcccg attgccgacc tgaccaaaac gcaggtttac cggcttgccg
    18601 aagcattggg cgtggacgag gcgattcaaa aagccccgcc gaccgacggc ctgtgggata
    18661 cggaacgcac cgacgaggaa cagatgggcg caagctatcc cgaactggag tgggcaatgg
    18721 gcgtgtacgg cacgcgcaaa cccgaagatt ttgaaggacg gcggcgcgaa gttctggaaa
    18781 tctatacgcg gcttcaccgc gcgatgcagc acaaaatcaa cccgattcct gtgtgccgca
    18841 ttccgcccga attgctgggc tgaaacatga aaatgccgtc tgaaacggaa aaaccgtatt
    18901 tcagacggca ttgaaatatc cgactcctat cccttaagaa tcgagtacgc gggcaaacag
    18961 aatatcgttt tccaaatgaa tgtggtcgtt caaatcgtcc gccatttctt ttgccagcgc
    19021 gtaaaggcgc gtccaacttc cgcaagcccc ttcgggtgcg tggaaattac cggtcagctc
    19081 tttgagccgt gcgatggcgc ggtcgtgttc ttcatgttcg tgcatcatca cgctgatggg
    19141 catcgccgca ccgcgtccga caccctgatt aatcatcgga aacagcatcc gctcttcctt
    19201 catcatatgc atcagcagct cgtcccgcat atcggcaagc agcccggcaa tttccgccgg
    19261 aaaggtatcg gcatggactt gggccacttt ctgcgccagc ggcaccaatt cttcaaattg
    19321 tgcgcggtgg acattgtggt agcgttgcag gatatgatca acggttgcgc caaagggagc
    19381 agcttcccaa acggaaaaat cagtcattgc agtgttcctt ttatagggtt tcgggtttgg
    19441 ttttgaacat tcatacttta agaatctatt taaacggagc atacaccgcc cgcgcgtttc
    19501 tgtacagcct caaaaacatt ccttatactt tgataataaa agtaattttc aaaaataaaa
    19561 tactgtccga accgtttttt agaatttgca aaggcgattg gggcggtaca gaaaaactat
    19621 tatcccgccc gcccacttga aatttttatg cccaagccct atcctgcacg ctatcgtgcg
    19681 aatcccaacc gaaaaggaaa aataatgagc agcgaattaa tcgtacacac cggcgatgcc
    19741 gccttcgagc aagatgtttt gaaatccgac ctccccgtcc tgctggactt ttgggctccg
    19801 tggtgcggcc cctgcaaaat gattgccccg attttggacg acattgccgc cgaatttgaa
    19861 ggccgtctga aagtagtcaa aatcaacatc gacgacaacg aagccacccc gtcccgtttc
    19921 ggcgtgcgcg gcattccgac cctgatggtg ttcaaaaacg gcgaagtcgt cgccaccaaa
    19981 gtcggcgcat tggcaaaagg tcagctgacc gcctttgtcg aagcctccat cgcctgataa
    20041 agcgcaatcg aaaagccgcc ggaagattcc ggcggctttt tcgcaccctt aagatttgtg
    20101 gcggatttcc caacaccggt ggattttttt gttgcggaaa tcttcgggaa cggattgttt
    20161 ggaaatgtct ttcacggcgt attgttccga taccgaatcg tccaaaacga agctgcgcag
    20221 gttgttggaa aaatacaaaa tgccgtctga agcgagcagg ttcaccgcgc cgtctatgag
    20281 tttttgatgg tcgcgctgga tgtcgaggat gccgggcatt ttcttgctgt tggaaaaact
    20341 gggcgggtcc atcacaatga ggtcgaactg cctgccttcc ccatatgccg tctgaaggta
    20401 ttggaacacg tcggcgcgga cgattttgtg ccgttccata tcgatgccgt tcaattcaaa
    20461 attgcgcctc gcccaatcga gataagtgtt ggacaaatcg acggtttcgc tggatgccgc
    20521 gccgccggtg gcggcataga cggtgaagct gccggtgtag gaaaacaggt tcaaaaaacg
    20581 tttgcccgcc gccgtttcgc cgactttttt gcgcgtgttg cggtgatcga ggaagaagcc
    20641 ggtgtcgagg tatttgtcga ggttgaccca aaacttgcgg ccgttttcgg caatgacgaa
    20701 atcgtcgccc gccttgccgg ttttctcgta ctgctgcaaa cctttttggc gttcgcggcg
    20761 tttgagacgg atttgttcgg gttcaaaacc ggtaacgaaa gcgacggctt ccaatacttc
    20821 ggcaagccac gcttcgtatt ctccgggccg catcagccag ccggtatcgt attcctgaag
    20881 gtggattcga tcgccgtaaa catcggcggc aaaagggaat tgggggatgt cgcggtcgta
    20941 aatgcgccag gcttcgatgc cgttgcgttt cgcccatttc ataaggtgtt tgatgttttt
    21001 gcccaagcgg ttggcaaacg gtgtgatgtc ggtcattgat ttcaggcgga ataaagtgga
    21061 aaacggaaat tttactgtaa ttaacgcccg attgcttgac cttttcgggc aaaccctata
    21121 ccatccgtcg cttatcttgt catacgaagc catcgccttc caacctaaac cgcccttacg
    21181 ggcgcgtttc ttctgttgct ttgattttgc aaagcatatc tgtgcaggtt gccgtcgatg
    21241 taaaccacaa gcaagccgct tgcgacaacc ctgtaacttc acattccccg tatcgttacc
    21301 cttccctgct tcaggccgtc tgaacctttc ggacgcgggc gttgttgtct tccaaggata
    21361 gccatgtcta ttaaatttgc cgatttgaac cttgataaaa acattttgtc cgccgtcagc
    21421 agcgagggtt acgaaagccc gacgccgatt caggcgcagg cgattccgtt tgctttggaa
    21481 ggctgcgaca tcatggcttc ggcgcaaacc ggctccggaa aaaccgcagc ctttctgtta
    21541 ccgactttgc agcgactgac caaacgcagc gaaaaaccgg gcaaaggccc gcgcgcgctg
    21601 gtgttggcac cgacccgcga actggcggcg caagtcgaga aaaacgcgct ggcgtatgcc
    21661 aaaaatatgc gctggttccg caccgtcagc atcgtcggcg gcacatcgtt cggctaccaa
    21721 acccgcgccc tgagcaaacc ggtcgatttg attgtcgcca ctccgggccg tctgacggac
    21781 ctgatgcaaa gcggcaaagt tgattttgaa cgtttggaag tgctgatttt ggacgaagcc
    21841 gaccgtatgt tggatatggg ctttatcgac gacatcgaaa ccatcgtgga agccaccccg
    21901 gccgaccgtc agactttatt gttctccgcc acttgggacg gcgcagtcgg caaactggcg
    21961 cgcaaactga ccaaagactc tgaaattatc gaagtcgaac gcgtggacgg ccaaggcaaa
    22021 atcgaagagc agttgttgta ctgcgacgat atgcgccaca aaaaccgcct gctcgaccac
    22081 atcctgcgcg atgccaacat cgaccaatgt gtgattttca cgtccaccaa agcgatgacc
    22141 gaagtcatcg cggatgaatt gtacgaaaaa ggtttcgccg ccaactgcct gcacggcgat
    22201 atgccgcagg gctggcgcaa ccgcacgctg acggatttgc gtaaaggccg ctgcaaaatt
    22261 ttggttgcca ccgacgttgc cgcacgcggt attgacgtac cgaccattac ccacgtcata
    22321 aactacgacc tgccgaaaca ggcggaagac tacgtccacc gcatcgggcg caccggccgc
    22381 gcaggccgca cgggtattgc gattacgttt gccgaagtga acgaatacgt caaagtccac
    22441 aaaatcgaaa aatacattaa ccgcaaactg cccgaactga ccatcgaagg catggagccg
    22501 gcccgcaaac gcaaatccgc aggcggcaag ccgaaaggca aaggcggctg gggcgatagg
    22561 aaatccggcg gccggcgcgg cgatcataaa ccgggcaaag aaggcttcgg cggcaaaacg
    22621 cgcggcgaag gtttcaagaa agaaggcttt aagaaagaca gtttcaaaaa aaccggcgaa
    22681 ggcttcaaag gcaaacgcaa agccggcgac tcttttgcag gcaaaagcga acgccgttac
    22741 aaagaccgct aagccccaac ctgccgcgta aaccaatgcc gtctgaaacc gatttcgagt
    22801 ttcagacggc atttttgtaa tgtttcagca ccgcccggct ttgataccca aaggattaat
    22861 ctgcaatata gtggattaaa tttaaaccgg taccgcgttg cctcgccttg ccgtactatc
    22921 tgtactgtct gcggcttcgt cgccttgtcc tgatttttgt taatccacta taaaaaccct
    22981 tttccgcttt ggcaacgatt gaaaatttcc gtaaattcaa atatctagat tccttcctgc
    23041 atgggaatga cgcagaaggg tttcagatgc aggatggaca ttcctgccta cccaatctcg
    23101 ccctacggct tgacggttcg atatgatgcc gtctgaaaac ccaacggcgg tatgacaatg
    23161 ccgccctgcc aacgcacgta gatcagaatt gccatcccga catcaaacgc ttggaaacaa
    23221 aatgccgtct gaaaatcaaa cggcaacata acaatgcccc taacaaatac aaaaatgccg
    23281 tctgaaagcc cttcagacgg cattgacgcg ccgggtttac cgccttctgc cgaaaccgcg
    23341 catagcggga cggcggtaat tgacgggcgg gcgcgttgtc gggcggtaac gctgcgcctg
    23401 cgccgcctgt tgttttgcac ggaggctgcg cgtgttcaaa tccctgctgg tgcgcgcatt
    23461 ggggcgtgcg gactgatggt aggcagcacg cgcgcgacgg gcgacggggc tgtcttggtt
    23521 gcccgcccgt gtgaatttgt ttgccagcgc gttgccgata aacgcgcctg ccgccgcgcc
    23581 aatcaggctt tgcagcagcc agcttcctgt cgattggtcg taaatatact gctgcccgtc
    23641 tttgccggta acgggctgac cgttgttgcc gtttgcctgc gcctgggcag gaatggtgtc
    23701 tttgactgct tcgggagtca gttggtaaac cgtatcgtct gcctgctgtg cgagctgctg
    23761 ttgcagggct tcaatctgtt tctgctgccg ttcgagccgc gcctgcgtgt cgtcttggca
    23821 ggcggtgagt gcgaatgttg cgataagcgc ggaggcgatg atttttttca tgtgtgtcct
    23881 gttcgggtgg aaaatcggtt gtattgtatc gccgtcggga attttggcaa gcattctgcc
    23941 ggcaaatcgt gatgtttaca ggggtagggt gtgcagtttg cggacaaatg cgaggctgtt
    24001 ggcgacaggg ttgcctttgt tttcgacttc gtgttcggtt tctatggtca gcaggcggcg
    24061 gtttttgtgt ttgttcaggc tcaattcggc ttgtgcgggt gtgaaaaaca ggcggtgttt
    24121 ttcggcaaag cggcaggcgc gcttgtttgc ggttttcaaa atctgaagca gcgatacgtc
    24181 cagatggaag cgtcgcacgc ccaatacgag aatccgtgcg gcaaaaaaat cggcggcatc
    24241 ggtaaactcg gcggggctgc tgcggctgaa gtcgtgccgt tcggcggcta tcagggcggc
    24301 aacggtgcgg atttgcggaa tcatcgattc gctgatgagc gtgtcgtccc gccctgcatc
    24361 gagaagcatg ggggaaaaat cctgcgcatc atcgacaata atgctgcaag tgtgcaggtt
    24421 ttcgttttgt acggcggttt ggggcattgc atccatggtc attttcctga ttctgtcgtg
    24481 tgttgccgaa tcgggcgacc tgtgtgaagg taacaaaaaa gccgccccgt tttcgagcgg
    24541 cctgttttgc gtatgggatg gatttcaagc aagcgcaaaa aagcaccgca cgtctgtgtg
    24601 ataccaatag cagtaagcgg ttgtaaatgt tttgccttgc atgatgaaat gccgtccgaa
    24661 gataaaaata ttggggagat tctaaatcaa aacgctgccg cgcctcaagc attatatcga
    24721 acttttttgt tttttcagct atccggttga aaatatttag gtttattttt accactgccc
    24781 gatattgtcg gcaatttccc tttatccgcc ttgaaaaacg gtgcataatc ccgagcaaaa
    24841 ccgcaatcag gagcaattat gcaaaactat ctgaccccca atttcgcctt tgccccgatg
    24901 attcccgaac gcgcttcagg cagccgcgtt tgggatacgg aagggcgtga atatattgat
    24961 ttttcaggcg gcatcgccgt taacgctctg gggcactgcc accctgccct tgtcgatgct
    25021 ttaaacgcgc agatgcacaa gctgtggcac atttccaata tctatacgac gcggccggcg
    25081 caggaattgg cgcaaaaatt ggtgaaacac agttttgccg acaaggtttt tttctgcaac
    25141 tcgggcgcgg aagcgaatga ggcggcgtta aagctggcaa ggaaatatgc gcgcgaccgt
    25201 ttcggcggag gcaaaagcga aatcgtcgcc tgtatcaaca gtttccacgg acgcacgctg
    25261 tttaccgtgt ccgtcggcgg acagccgaaa tacagtaagg attatgcacc gctgccgcaa
    25321 ggcattacgc acgttccgtt caacgatatt gccgcgctgg aagctgccgt cggcgaacag
    25381 acctgcgcgg tcatcatcga gccgatacag ggcgaaagcg gcatcctgcc cgccactgcg
    25441 gaatatttgc aggcggcgcg ccgtctgtgc gaccggcaca atgcgttgtt gattttggac
    25501 gaagttcaaa ccgggatggg gcatacgggc aggctgtttg cctatgaaca ttacggcgtt
    25561 gttcccgata ttttgagttc ggcaaaagcc ttgggctgcg gctttccgat cggcgcgatg
    25621 ctggcgacag aaaagattgc cgccgccttc caaccgggca cgcacggctc gactttcggc
    25681 ggcaacccga tggcgtgtgc ggtcggcagc cgcgccttcg acatcatcaa cgcgccggaa
    25741 accttgcaca acgtccgcag tcaggggcag aaacttcaga cggcattgct ggatttgggc
    25801 aggaaaacag gcttgttctc acaggttcgc gggatggggc tgctgctcgg ctgcgcgttg
    25861 gacacgcctt atcgcggacg ctcatccgaa atcgccgcta cctccttgaa acacggcgtg
    25921 atgatcttag ttgcgggtgc ggacgtattg cgtttcgcgc cttcgctgct gttgaacgat
    25981 gaggatattg cggaaggttt gcggcgtttg gaacacgtgc tgacggaatt tgccgccgca
    26041 aaccgcccct aggcagaaag cagatgccgt ctgaagacgg gaaggcctca gacggcatca
    26101 gaaacaaaaa accgcttcgg aaacgtgatc caacgttccg aagcggtttt gtttgccatc
    26161 aggactcgaa caccaattcc ggttccctgc cctcttcgat gactgccttg ccgaccacca
    26221 cttttttcaa gccttgcaaa tcgggcaggc ggtacatcgt atcgagcagg cagcgttcga
    26281 cgatggaacg caggccgcgc gcgccggttt tgcgttccat tgcctgccgc gcgatggaac
    26341 gcaatgcgcc ttcttcaaat tccaacccga cgttttctat gccgaacaag gcttgatact
    26401 gcttgaccaa agcgtttttc ggctcggtta aaatattaat cagcgcgtcc tcatccagtt
    26461 cttctaaagt tgcaatcacg ggcaaacgtc cgattaattc cggaatcagg ccgaatttga
    26521 ttaaatcttc cggttcgacg atgccgaaca gcttggtaat gccggcattt tcgtccttgc
    26581 tgtgaacgga cgcgccgaaa ccgataccac ctttctcggt gcgctggcga atcacttttt
    26641 ccaagcctgc aaacgcgccg ccgcagataa acaggatgtt ggcggtatca acgttgataa
    26701 attcctgatt cggatgcttg cgtccgcctt ggggcggaac gcttgccacc gtaccttcaa
    26761 tcagtttcag caaggcttgc tgcacgcctt cgccggacac gtcgcgcgtg atggacgggt
    26821 tgtcgctttt acgcgaaatt ttgtcaattt cgtcaatata gacaatgccg tgctgggctt
    26881 tttcgacatc gaaatcgcat ttgcccaaca gtttggtaat gatttgttcg acatcttcgc
    26941 cgacatagcc ggcttcggtc agcgtggtcg catccgccat cacgaacggc acgtccagtt
    27001 tgcgtgccaa agattgcgcc aacagcgttt tacccgatcc ggtcgggccg ataagcagga
    27061 tgttggattt cgacaattcg acaccgccgt cggctttcgg gtggcgcagg cgtttgtaat
    27121 ggttgtaaac cgcaaccgcc agcgcctttt tcgcctgccc ctgcccgatg acataatcgt
    27181 cgaggttggc aacgatttcg gcgggcgtgg gcagtttgcc ggattcttcc ggctcccctc
    27241 cggcattttc cggaggcgtg ccgccgttgt cgccttcgta caatatttcg aggcagtttg
    27301 cgacgcattc gtcgcagata taggcgtttt tgccctcaat caggtgtttg gcgtgtgatt
    27361 tggattttcc gcaaaaggaa caggtacggt tttcgttgga catggctttc tttacaatgt
    27421 gtgcgttgca gaaacggcat gcgccgttcg ggttgccaag tataataact atatccgtgc
    27481 ttatcaacgt attaccttaa aatcccgccg attcggctat aatacgccct ttcgcaaccg
    27541 ccccggcggc agaaatgccg tctgacacca aatctgagga tattcatgag aaaaccccaa
    27601 cgcggctacg cccgccaaga ccgtgtcaaa gaacaaatta tgcgcgagct tgccgaactc
    27661 gtccgtaccg gactgaaaga cccgcgcgcc ggcttcatca ctatcaacga agtcgaagtt
    27721 acccgcgatt acagccacgc caccgtgttc tacaccgttt tgaaccaaga tacgcgcgaa
    27781 attacggaag aagtgctgga acacgcgcgc ggacacctcc gcagcgaatt gtccaaacgc
    27841 atcaagctgt tcaaaatccc cgaactgcat ttcaaatacg acgaatcttt ggaacgcggt
    27901 atgagcctgt ccgcccttat cgatcaagtg gcggcggaaa aaccggttga agactgatac
    27961 tgccagaatg aacaatctga ttcccgaaca tttagccgcc tatgcacata gtgataacct
    28021 gcaaattgaa ggcgggcatc gttgcttttc attatcctgc caaggtagag atactttcca
    28081 catccgttac tatggagagc cttttgatgg attgattacc gatactgata aggcgccggt
    28141 aaaaattgtg gcggtagaag ctgtaagcgg cgatgaaatc gtattgtttg atggggcgga
    28201 acatggctat aacgctatgt tttgcgacaa atatagccaa aatcaaaagc aaaacagaac
    28261 gttaactgat ttggatgaat atacttaccg agttccgatt catctttatt acaatataga
    28321 ctacgaagat gaatatgaag atttcgtcaa ttctgaagga caagtcccct taattgatgg
    28381 ccgcatcatt agttttgact cattaaaacg aaatggcttt gatgcaatca gcattgatct
    28441 aattgatgaa aaacactctg ttcgtgaatt attgaatgaa gaattaagct gacacgcagg
    28501 ccgtccgaac cctttcagac ggtctgaaca ttaatattcc ctaattaata tgaccaataa
    28561 acccgccaaa cgcccggtca acggcgttct tcttctcgac aaacccgaag gcctttccag
    28621 caacaccgcc ctgcaaaaag cgcggcgttt gttccatgcc gaaaaagccg gacataccgg
    28681 cgtgctcgat cctttggcaa ccggactttt gcccgtctgc ttcggcgaag cggccaagtt
    28741 cgcccaatat ctgattgatg ccgataaagc ctacaccgcc acgctgaaac tcggcgaagc
    28801 cagcagcacg ggcgatgccg aaggagaaat cattgccgcc gcccgcgccg atatttcctt
    28861 agccgaattt cagacggcct gccaagcact gacaggcaac atccgccaag tgccgccgat
    28921 gttttccgcc ctcaagcacg aaggcaaacc gttgtacgaa tacgcgcgca aaggcattgt
    28981 tatcgaacgc aaaccgcgcg acattaccgt ttactctatc gatattgccg agtttgacgc
    29041 gcccaaagcc gtgataagcg tgcgttgcag caaaggcacc tacatccgca ccctcagcga
    29101 aggcatcgcc aaacacatcg gcacattcgc ccacctgacc gccctacgcc gcaccgaaac
    29161 cgccggcttt accatcgccc aaagccacac gctcgaagct ttggcaaatt taaacgaaac
    29221 agaacgcgac ggtctgctgc tgccctgcga cgtattggtt tcacactttc cccaaaccgt
    29281 tttaaacgat tatgccgtcc atatgctcca atgcgggcaa cggccgcgtt tcgaggaaga
    29341 cctgccttcc gatacgccgg tacgcgttta cacggaaaac ggccgctttg tcggtttggc
    29401 ggaatatcaa aaagaaatat gccgtatgaa agccttgcgc ctgatgaaca cggcggcatc
    29461 ctccgcctga gcggcggtta aaaatacggg ctgcgtttga ataatgtgtt gatatttccg
    29521 taaaatcacg acacactcgg acacccgccc cgcttatcgc aactttgcga acttcccccg
    29581 gaaacagcaa agacatcaaa gaattgattt tattataatt tatttgtaaa gccatttgcc
    29641 gttacacaag aatggcacat taaaataact gatgaggatt tataacgatg aagacaggca
    29701 ttcaaaccga attagcccaa gccctgctat cacacgaaaa aatatgggcg aacgaagaaa
    29761 aaaccatttt ggccaaaaac atcctgttgg atttggtgga aaaaaccgac ccgtccatta
    29821 tcggtttgtt attgaataat gatgagttaa aacgccattt ctttgtggaa gtgaatggtg
    29881 ttttggtgtt taaattgcag gatttccgtt ttttcttgga caaacacagc atcaataatt
    29941 cctacacaaa atacgccaac cgcattggtt tgacggacgg caaccgcttt ttgaaagaca
    30001 attcggatat tgtgttggat tttccgttta aagattgcgt attgaatggc ggacaaagca
    30061 ccgaagaagg cgaagaaatt tattttaaac gcaataatag ccagccagcc agccagccag
    30121 ccagccagcc agccagccag ccaattatac accaaattaa ccagaaaaag acaagaaatc
    30181 ttttttaatc aaacgcttgc ttttgatgaa attgaccggc tttttgacgc aaaagcattc
    30241 tcaaaattct cccgccacac cgcagacggc aaacaacccg ttggcgaaat caaacgccgt
    30301 tcagacggta cgccagccga aaatcttatt atcaaaggca ataatctgat tgccctgcat
    30361 tcgcttgcca agcagtttaa aggcaaagta aagttgattt atattgatcc accgtataat
    30421 acagaaaccg atagctttgc atataacgat aaattttcac attccacttg gctaacattt
    30481 atgaaaaatc gcttggaaat tgcgaaagag ttattaaaag atgatgggct aatctttgtt
    30541 caatgcgatg ataaggaaca ggcttattta aaagttttat tagatgaaac ttttacaaga
    30601 gaaaatttta taaactgcat tgctgtaaaa atgtcagagc catcaggtaa caaaatggca
    30661 catacatcgc atagactacc aaagataaag gaatatattc ttatctataa gaataaaaat
    30721 ataaaattaa accctattag agagcaaaaa tcagaatggg acaatgagta caatattttt
    30781 ttagaaaatt ttacacaaga agataaaaaa tttattgatt taatagtaaa tagccaaaca
    30841 gaaaataaag aaataaatgg taatacttta aaagaaattg atattctttt aaagaaaata
    30901 tctcctattt cagttaatca aaaattagcc caattaaata ttaaagataa tgaagttata
    30961 aaatggaaac tagataatgc gtatagaatt gtaagaacag cagctagtag tagcgtaaaa
    31021 aaattagcag atgaaaaaaa agaaatttgc caacaacaat ttttttcagt tatttctaaa
    31081 cgagataaat tattgtatat cgtcaagtca gattactcaa aagatgcaaa agcaccacga
    31141 gtgcaggttt tgtttgcaga agattatttg agtattagtt tatgcgattt atggacaaat
    31201 ataaatacca caggactaga agcagaagga aatgtagagt taaaaaatgg taaaaagcca
    31261 gaaagtttaa ttgaaaccat cattaaacta gcaaccaacg aaaacgacat cgttctagac
    31321 taccacttgg gcagtggcac aaccgccgcc gtcgcccata aaatgaaccg ccaatacatc
    31381 ggcattgaac aaatggatta tattgaaacg cttgccgttg aacgcatgaa aaaagtaatt
    31441 gacggcgagc aaggcggtat ctcaaaagct gtgaattggc aaggtggcgg cgagtttgtt
    31501 tatgccgaac tttccccatt taacgaaacc gcaaaacaac aaattttggc gtgcgaaaat
    31561 tcagacggca tcaaaacact gtttgaaggt ttatgcgaac gccatttctt gaaatacaac
    31621 gtcagcgtaa atgaatttag ccaaattatt gaagagcctg aatttcaatc cttggcatta
    31681 gacgaacaaa aacaaatgat gcttgaaatg ttggatttaa atcaaatgta tatttcatta
    31741 tccgaaatgg acgatgaaca atttgcaggc tgcctgaatg atgatgataa agcgttaagc
    31801 cgtgcgttct atcaatcagt aaaacatcaa gcggagaaaa aagatggcga ataataaaac
    31861 gttgtttgat tgggtggaag accgcaaatc aatgctggaa gaaatggaac agacggattt
    31921 tttcgcgctg cctgaatttg tttccaataa tttaaaatat ccgttttttg aatggcaaaa
    31981 atcggcttta gaaaattttg tgatttttga ccgcacttca aaattaaagg atttccccga
    32041 catcaaaaac cgccctaccc atttgctgtt caatatggca acaggtgcag gcaaaacgat
    32101 gatgatagcg gcattgattc tgtattattt tgagaaaggg tatcggcatt ttttattttt
    32161 cgtcaataaa aacaatatag tatataaaac ggaaaataat tttatcgacc cgacgcatcc
    32221 aaaattttta tttaccgaga aaattttgca aggtgatacg gtaattccca ttcgcaaagt
    32281 ggagacattt agccaatatt cagacggcat tgaaattaaa tttaccagca ttcaaaaact
    32341 atataacgat attcacaccg agcgggaaaa tcaaaccaca ttggcggatt tgcacgaatt
    32401 aaatcttgtg atgctgggcg atgaagcaca ccatttaaac gcgcaaacca aaggcaaaaa
    32461 acaaggcgaa ttagatttag aaaaggaaat gaacgagcgt accagcgatg ccgaatttga
    32521 acgcaaaggc tgggagcata tggttttgga gttgttactc aataaaaatg gcaatcccgg
    32581 ccaaaatgtg ctgttggaat ttaccgccac gctgcctgaa aatactgaag tgcaacaaaa
    32641 atatgctgat aaaatcatca caaaatttgg tttaaaagaa tttttgcaaa aaggttatac
    32701 caaagaaatc aatttggtat ccggtacgct gaataagaaa gagcgggtgt tgcacgcttt
    32761 attgtttgcc tggtaccgcc atcaaatcgc gttgaaatac ggcattgcca atttcaagcc
    32821 tgtgatgttg tttagaagta agacgattga tgaatcaaaa gcggattatt cggcgttttt
    32881 aaattgggta gaaaatgtgc aagcggatga tttttcgttt ttaactacat tttcaacaag
    32941 cttaaacgat agcaataacg ccaacgaaca aggcaaaacc cgtaccgaac aagccctgaa
    33001 atttatgcag gaaaataaat ttgaatttgt gcatttggca aattggatta aacaaaatta
    33061 tcaaaaacac aatgtcatta ttaccaactc cgaaaccaac aaaaacaaaa ctgaaaaaac
    33121 cgacagcgaa accgaaaaat tgctgaacaa tctggaagcg gctgataatc cgatccgtgc
    33181 catttttacg gtggatagat taaccgaagg ttgggacgtt ttaaatttgt ttgatattgt
    33241 gcgtttgtat gaagggcaaa acggtggcgg ttcgaataaa aaatcaggca aaacggctgc
    33301 cgccaccgta tcggaaaagc agttgattgg tcgcggcgtg cgttattttc catttgcgtt
    33361 tgaaggtaaa cagccgaata aacgcaaatt tgacaacgat atgcaacatg aattgcgtat
    33421 cttggaagaa ttgttttatt acacgcacga tgagcaatcc cgctatatta cagaactgaa
    33481 aaacgagtta cgaaaagacg gttatttgcc tgaaaaagac gatgataagg tattggcaac
    33541 atttaagctc aaatctgaat ttgcagataa taaggatttt agagagttgt taatttgggc
    33601 gaataaaaaa atccccaacc caaacgccaa aagcaataat gcagacagcc tgcaagccaa
    33661 tccgcaaacg ctttcattcc aagttcacgg taatcaactg ttgcaggaaa cgcaatttac
    33721 agccgatgaa aatgatgaaa cagcccgaca aatcggcaca caaaataatt ttactcaaac
    33781 cataaaaatg agtgaaatgg agcggcatat tttcaataaa gcattacata tcaaagggaa
    33841 aaatagtcaa tctttattcc attttgaccg cctgcaaagc aaactcgaca ttcaaaatcg
    33901 caatgaattg caaaataaat tgttgaaaga ctggcagatt gaatttttgg gattagagca
    33961 agataaacag gttcgcccgg atgataaact tgcaggctgc ctgaaaatct tggaaatggt
    34021 tgaaaaacat ctgaatgaaa gtgatatacc gtttatcggt acaaaagaat ttacacctaa
    34081 aaaattgtgg gaaattttcg gcacgccaaa acaaaaatgg gtcaaaaaag atgatataaa
    34141 aactgctatt gccacgcaaa atgattggta tgtgatggat aattttgccg gaacgggttt
    34201 ggaagaagcg ttaattcaat ttatttcagc gcgcttgggc gatttgaaat ctcaatatga
    34261 cgttcattta atccgcaatg aagaagtgtt taaattgaat aactttgccg atggcgaagg
    34321 atttatgccg gactttgttt tattgctgaa agataaacaa aaatcttctt ccgacagcgt
    34381 ggacgacttt ttgcattacc aaattttcat tgaaccgaaa ggcgggcatt tagtggaaaa
    34441 tgattcgtgg aaagaagctt ttttaaaatc aatcacagtg gaatatggga gggataagat
    34501 cctgcaaaaa aatacaccgc attatcgttt gatcggtttg ccgtttttta ctgatcatca
    34561 gaaaaatgga caatttacag agttattccc tttaggtgcg gcatcgcttg aaaaatagag
    34621 tagtgcattg caggcagccc cgttcggcaa aacttccttt acaaaagggc gttttgtcag
    34681 atatttaatc aacacattat taaaatacaa ccaaatttta atgccgtccg aaccatgtat
    34741 tcagacggca tcgtattttt cagtatctaa accgtttccc tgccccaatc cttactcctc
    34801 aaaatcgagg catcgacatc ttgaatatcg cggtgtcccg taaacgccat cgacacatcc
    34861 atttccttgt acaaaatctc cagcgcgcgc gttacgcctt cttcgccata cgcacccaaa
    34921 ccatacagga acgcccgacc tatcatcgtg cctttcgcac ccaaagccca cgccttcaaa
    34981 atatcctggc cgctgcggat accgctgtcc atccaaactt cgatgtcact gccgactgcg
    35041 gaaacaatat cgggcaaggc cttgatggcg gacacagtat cgtcgagctg gcggccgccg
    35101 tggttggaaa cgaccaatgc atccgcaccg cttttcgccg ccttttccgc gtcttcgggt
    35161 tccataatgc ctttgataat cagcttgccg ccccacaaat ctttaatccg cgcaacgtcg
    35221 tcccagctta agcgcgggtc gaactgttcc gccgtccacg aagaaagtga agacaaatcg
    35281 ccgacgtttt tggcgtgtcc gacgatattg cggaacgtgc ggcgttccgt gttcagcatt
    35341 ttcatgcacc attcgggctt ggtcgccaga ttgattaaat tggcgatggt cggtttcggc
    35401 ggcgcggaca ggccgttttt gatgtctttg tggcgttgac ccaaaacctg caaatcggca
    35461 gtcaatacca atgccgaaca tttggcatct ttcgcacgct taatcaggtt ttccataaat
    35521 tcgcggtcgc gcatcacata aagctgaaac caaaacggcg cgctggtgtt ctcggcaacg
    35581 tcttcaatcg agcagatgga catggtggac aacgtaaacg gaataccgaa cttctccgcc
    35641 gcacgcgccg ccaaaatttc gccgtctgcg tgtgccatgc cggtaaaacc cgtcggcgca
    35701 atcgccaccg gcattttcac atcccccccg atcattttgg tttcgaggct gcgtccctcc
    35761 atattgacca ataccttttg tcggaagcgg atatctttga aatccgaagt attctcccta
    35821 taagtcgttt ccgtccacga acccgaatcg atgtaatcgt aaaacatacg cggcattttg
    35881 cgcttggcaa cgcggcgcaa gtcttcgatg caggtcattt tgctcaaatc acgtttcatc
    35941 ttgtttttcc ctgtttaaca gcctgatgga atatgccgtc tgaaactgct tgagccttca
    36001 aacggcattt cgggcaatac ccaaataaat cactaagatt tagataatct atatcaatat
    36061 taaaaataaa tcaaatcgtt ttgaaaccgc ttgccggcaa aacgccccaa ccgccgctcc
    36121 tcaaaaaaca taactaactg attcaaaaaa ccaaacaaat aaagcaaaat cagactatac
    36181 cgccatttaa acaaacaatc ggcaacagct cccccatact tgactgaaac actcagatat
    36241 tggacaattc cgcccactat aaaaaagccg acacgggcaa ccaccaccat gagactgacc
    36301 accaaagggc gtttcgccgt taccgctatg ctggatttgg cgatgaacgc gcaaaccggc
    36361 gccgtcaaac tcagtgccat cagcgaacgc caaaatatat ccctctccta tctcgagcaa
    36421 ttgttcggca aactccgccg cgccggactg gttgaaagcc tgcgcgggcc cggcggcggc
    36481 tacatcctcg ccgcccctcc cgcacaaatc aacatcgccc aaatcatttc cgccgccgaa
    36541 gaccgtctgg acgcgaccca atgcggcagc aaagccaact gccaccacgg cgcgccctgc
    36601 ctgacgcacg acctttggga gaatttaaac aaaaccatca acgactatct cggcagcgtt
    36661 accctgcaaa gcatcatcga acagaaaaac aacggcgacg gcagccgcgt cgtccaattt
    36721 acacacatcc attaaataac acccgaaaaa gaaagagcaa accatgaccg tcaaaacccc
    36781 cgtttacctc gactacgccg ccaccacacc cgttgacaaa cgcgttgccg aaaaaatgat
    36841 tccctatctg actgaaacct tcggcaaccc cgcctccaac agccacgcat tcggctggac
    36901 ggcagaagaa gccgtcgaaa aagcccgcgc cgacatcgcc gccctgatta acgccgaccc
    36961 caaagaaatc gtcttcacca gcggtgcgac cgagtccgac aacctcgcca tcaaaggtgc
    37021 ggcaaacttc tacaaaacca aaggcaaaca cctcatcacc gtcaaaaccg aacacaaagc
    37081 cgtgctcgac acgatgcgcg aactcgaacg ccaaggcttt gaagttacct acctcggcgt
    37141 gcaggaaaac ggtttgattg atttggaaga actcaaagcc gccatccgcg acgacaccat
    37201 cctgatttcc ataatgtggg tgaacaacga aatcggcgtg gtgcaaaaca ttcccgccat
    37261 cggcgaaatc tgccgcgaac gcaaaatcgc cttccacgtc gatgccgccc aagcctgcgg
    37321 caaagtgcct gtcgatgtcg aagccgccaa aatcgacttg ctctcgatgt ccgcgcacaa
    37381 agtgtacggc cccaaaggca tcggcgcgct gtacgtccgc cgcaaaccgc gcgtccgcct
    37441 cgaagcccaa atgcacggtg gcggtcacga gcgcggtttc cgttccggca cattgccgac
    37501 ccatcaaatc gtcggcatgg gcgaggcctt ccgcatcgcc aaagaagaat tggcacaaga
    37561 cacagcgcac tacctgaaac tgcgcgacat cttcctcaaa ggcatcgaag gcatcgaaga
    37621 agtctatatc aacggcgacc tcgaacaccg cgccccgaac aacctgaacg tcagcttcaa
    37681 cttcgtcgaa ggcgaaagcc tgattatggc ggtgaaagaa ctcgccgtat ccagcggatc
    37741 cgcctgcacc tccgcctcgc tcgaacccag ctacgtcctg cgcgcgcttg gccgcaacga
    37801 cgaactggcg cactcatccc tgcgcatcac cttcggccgc atgaccaccg aagaagaagt
    37861 gcaattcgcg gcagaactga tcaaatccaa aatcggcaaa ctgcgcgaac tgtcgccgct
    37921 gtgggaaatg ttcaaagacg gtattgatct gaactcgatt gaatgggcgg cgcattaaga
    37981 ttgatgtcaa acaaaggctg tctgagaaag aataccatga aagttaaaat tattaatttg
    38041 ccaaatggtt ataaacgtat tatttatgga aaatattttg aacaatttga cttagattat
    38101 gaacaagatt tagacgtatt aaaaaaagat attgaatttg ctttatctgt tattgaatac
    38161 aatcgatcta tattcaaaaa attctcttcc ttatttgaga ataaaatcat ttttgtttat
    38221 caaggaggac atcatcttga tattattgat cgtgataaag gaagtttgaa atagttaact
    38281 attgaagatc attggacaaa agatgttgat gatatcactg gttcatggaa aatggagaat
    38341 ttagaaatta gccgacaaac tcaatatgca atgcaacttc taaagattgg taaaacaaat
    38401 attgagaaag aaataagaaa aattaaacaa aggttctaaa aaaatgagta aatacggtgg
    38461 tgaagacgta atctatatga atgatggtgt atttagaaat aaattagcca ttcaagatcc
    38521 acttaaattt aaaaaaggtc gaaagagaca tttctgctat ttccgcccaa aatctgcttt
    38581 tgcaacccat tacaggcaat ttcgatttgg cgcatttaca gaccattcat cgcgaactgt
    38641 ttggcaatgt ttatgattgg gcaggaaaaa tccggagggt tgatatttct aaaggtaata
    38701 cccgatttgc caattttgct tttatcgaaa acgaaagccg taaacttttg gagaagctga
    38761 aaaacgaaaa ctatttgcgg gtgttggata aagataaatt cgccgagcgt gcagcctatt
    38821 atttggacga gttgaacgtc ctacatcctt ttcgtgaagg aaacggcaga acattgcgcc
    38881 tgtttatgac acaattggca ataaaaaacg gttttcagat acattggcaa aatatctctg
    38941 ccgaacaaat gattcaagcc tgtattcagg cataccatgc cgatagcagc ttattggcac
    39001 gcctaattat tagacaattt agaacaatcg tctttttcag acgactaaaa catcaacaaa
    39061 ccacgatatt ttaaggaaac cacatcatgg catacagcga taaagtaatc gaccactacg
    39121 aaaatccccg caacgtcggc actttcgaca aaaacgacga gtccgtcggc accggcatgg
    39181 tcggcgcgcc cgcctgcggc gacgtgatgc gcctgcaaat caaagtgaac gatgaaggca
    39241 tcatcgaaga tgcgaaattc aaaacttacg gctgcggttc cgccatcgct tcgtccagcc
    39301 tgattaccga gtgggtcaaa ggcaaaagtc tggatgacgc gctggcaatc aaaaacagcg
    39361 aaatcgccga agaactggaa ttgccgccgg taaaaatcca ctgctccatc ttggctgaag
    39421 atgcggtaaa agcggccgtt gccgactacc gcaaacgtca ggaaaacaaa taaagccctt
    39481 cagacggcat cgtcccacaa tgccgtccga accgcccgcc gcttcggatc cgtccggggc
    39541 ggtacaacaa ggaagaaata tgattaccct taccgagaat gccgcaaaac acatcaatga
    39601 ctatctcgcc aaacgcggca aaggcttggg cgtacgcttg ggtgtaaaaa ccagcggctg
    39661 ctcggggatg gcgtacaacc ttgaatttgt cgatgaagcc aacggcgacg acctgatttt
    39721 cgaaggacac ggcgcgcgca tttatatcga cccgaaaagc ttggtttatc tggacggcac
    39781 acaagtcgat tacaccaaag aagatttgca ggaaggtttc aaatttgaaa accccaatgt
    39841 caaagactcc tgcggctgcg gcgagagctt ccacgtttaa ggcataaaaa cggcgggaca
    39901 gtatcaaaac cgtcccgccg ttttttcgct tcctgcctgt tatagctgcc tttgcctttc
    39961 cttttccgtt ccaccttgtg ccggaacaaa tcggatttta ctaaggcttt taaagcattg
    40021 tcgcgtattt tgcctttatt gtgctgcgct ttgccgccca tattcagtcc tttcgtttaa
    40081 gaagccgcag attataaggc aaaaacagtt ttctgccaaa atcttacatt tatcatccta
    40141 ctatgtccca atatttcacc ctcttccgga ttgaacccgc tttcgatatc ggcaccgaaa
    40201 acttggaaca aacctaccgc gccttggccg cccgtttcca tcccgacaaa ttcgcttccg
    40261 cttccgcttt cgagcagaaa caggcggtta tgatgtcttc caccatcaac gatgcctacc
    40321 gcaccttgaa aaatcccatc gaccgcgccg cgtacctgct gaaaacatcg ggtatcgatg
    40381 ccgacgcgcc ggaacatacc tctttcgccc ccgacttcct catgcagcaa atggaatggc
    40441 gcgaaacgct gatggaggca cgggcaggca acaaccttga atccttgaaa aatctcgata
    40501 acgaaatccg cgccgaacaa gaaaaactgt tctgcggtct gaaacagtca ttcgcgcgcc
    40561 aagactgcga caccgccgca caacaagtcc gccaaggcag gtttctcgac aaactccgcc
    40621 acgaaatttc ctccgcatta taatccgcac cgtgtttcag acggcataac cgccgcaccg
    40681 tttcatgtcg aaatatgcta aaataagcaa caattttttg ccatacgaaa cattgaaacc
    40741 atgaccgacg caaccatccg ccacgaccac aaattcgccc tcgaaaccct gcccgtcagc
    40801 cttgaagacg aaatgcgcaa aagctatctc gactacgcca tgagcgtcat tgtcgggcgc
    40861 gcgctgccgg acgttcgcga cggcctaaag ccggtgcacc ggcgcgtact gtacgcgatg
    40921 cacgagctga aaaataactg gaatgccgcc tacaaaaaat cggcgcgcat cgtcggcgac
    40981 gtcatcggta aataccaccc ccacggcgat ttcgcagttt acgccaccat cgtccgtatg
    41041 gcgcaaaatt tcgctatgcg ttatgtgctg atagacggac agggcaactt cggatcggtg
    41101 gacgggcttg ccgccgcagc catgcgctat accgaaatcc gcatggcgaa aatctcacat
    41161 gaaatgctgg cagacattga ggaagaaacc gttaatttcg gcccgaacta cgacggtagc
    41221 gaacacgagc cgcttgtact gccgacccgt ttccccacac tgctcgtcaa cggctcgtcc
    41281 ggtatcgccg tcggtatggc gaccaacatc ccgccgcaca acctcaccga caccatcaac
    41341 gcctgtctgc gtcttttgga cgaacccaaa accgaaatcg acgaactgat cgacattatc
    41401 caagcccccg acttcccgac cggggcaacc atctacggct tgggcggcgt gcgcgaaggc
    41461 tataaaacag gccgcggccg cgttgttatg cgcggtaaga cccatatcga acccataggc
    41521 aaaaacggcg aacgcgaagc catcgttatc gacgaaatcc cctatcaggt caacaaagcc
    41581 aagttggtcg agaaaatcgg cgatttggtt cgggaaaaaa cactggaagg catttccgag
    41641 ctccgcgacg aatccgacaa atccggtatg cgcgtcgtta tcgagctgaa acgcaacgaa
    41701 aatgccgaag tcgtcttaaa ccaactctac aaactgactc cgctgcaaga cagtttcggc
    41761 atcaatatgg tggttttggt cgacggacaa ccgcgcctgt taaacctgaa acagattctc
    41821 tccgaattcc tgcgccaccg ccgcgaagtc gttacccgac gtacgctttt ccggctgaag
    41881 aaggcacgcc atgaagggca tatcgccgaa ggcaaagccg tcgcactgtc caatatcgat
    41941 gaaatcatca agctcatcaa agaatcgccc aacgcggccg aggccaaaga aaaactgctt
    42001 gcgcgccctt ggcgcagcag cctcgttgaa gaaatgctga cgcgttccgg tctggatttg
    42061 gaaatgatgc gtccggaagg attggctgca aacattggtc tgaaaaaaca aggttattac
    42121 ctgagcgaga ttcaggcaga tgctatttta cgcatgagcc tgcgaaacct gaccggcctc
    42181 gatcagaaag aaattatcga aagctacaaa aacctgatgg gtaaaatcat cgactttgtg
    42241 gatatcctct ccaaacccga acgcattacc caaatcatcc gtgacgaact ggaagaaatc
    42301 aaaaccaact atggcgacga acgccgcagc gaaatcaacc cgttcggcgg cgacattgcc
    42361 gatgaagacc tgattccgca acgcgaaatg gtcgtgaccc tgacccacgg cggctatata
    42421 aaaacccagc cgaccaccga ctatcaggct cagcgtcgcg gcgggcgcgg caaacaggcg
    42481 gctgccacca aagacgaaga ctttatcgaa accctgtttg ttgccaacac gcatgactat
    42541 ttgatgtgtt ttaccaacct cggcaagtgc cactggatta aggtttacaa actgcccgaa
    42601 ggcggacgca acagccgcgg ccgtccgatt aacaacgtca tccagctgga agaaggcgaa
    42661 aaagtcagcg cgattctggc agtacgcgag tttcccgaag accaatacgt cttcttcgcc
    42721 accgcgcagg gaatggtgaa aaaagtccaa ctttccgcct ttaaaaacgt ccgcgcccaa
    42781 ggcattaaag ccatcgcact caaagaaggc gactacctcg tcggcgctgc gcaaacaggc
    42841 ggtgcggacg acattatgtt gttctccaac ttgggcaaag ccatccgctt caacgaatac
    42901 tgggaaaaat ccggcaacga cgaagcggaa gatgccgaca tcgaaaccga gatttcagac
    42961 gacctcgaag acgaaaccgc cgacaacgaa aacaccctgc caagcggcaa aaacggcgtg
    43021 cgtccgtccg gtcgcggcag cggcggtttg cgcggtatgc gcctgcctgc cgacggcaaa
    43081 atcgtcagcc tgattacctt cgcccctgaa accgaagaaa gcggtttgca agttttaacc
    43141 gccaccgcca acggatacgg aaaacgcacc ccgattgccg attacagccg caaaaacaaa
    43201 ggcgggcaag gcagtattgc cattaacacc ggcgagcgca acggcgattt ggtcgccgca
    43261 accttggtcg gcgaaaccga cgatttgatg ctgattacca gcggcggcgt gcttatccgt
    43321 accaaagtcg aacaaatccg cgaaaccggc cgcgccgcag caggcgtgaa actgattaac
    43381 ttggacgaag gcgaaacctt ggtatcgctg gaacgtgttg ccgaagacga atccgaactc
    43441 tccggcgctt ctgtaatttc caatgtaacc gaaccggaag ccgagaactg aaaatcatct
    43501 cccgatgccg tctgaagatt cagacggcat ttatttttcc ctcatccgtc atccagcttc
    43561 tcacaatata gcggattaac aaaaatcagg ataaggcgac gaagccgcag acagtacaaa
    43621 tatagtggat taaatttaaa ctagtacagc gttgcctcgc cttgccgtac tatagtcaat
    43681 taaaaacaag gg
//
LOCUS       DAWTQS010000008        41563 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.008, whole genome shotgun sequence.
ACCESSION   DAWTQS010000008 DAWTQS010000000
VERSION     DAWTQS010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 41563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41563
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.008"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            <1..796
                     /gene="pglE"
                     /locus_tag="WNA25_000453"
     CDS             <1..796
                     /gene="pglE"
                     /locus_tag="WNA25_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="(galactosaminyl)2-N,
                     N'-diacetylbacillosaminyl-alpha1-diphospho-di-trans,
                     octa-cis-undecaprenol synthase PglE"
                     /protein_id="HFA9823286.1"
                     /translation="PNTKYIYQDNQGLSEARNTGIKNSNGKYIAFIDSDDFINCQVLL
                     DFLGKDDSDMPDVVFLNAVKYDKGRVSYFGEDYQPEKILNQSKVEVLKGLCRFRKFPG
                     SAWNKIIKRELIIREKLFFEKGIYSEDIEWSMRLFNAATTFSYLDGCYYYYRQGRKDS
                     ITGTVSEKGIKSLLYILEKNAKMEFNRDISSYLYSFLSYEYLVLLFIMTSKNIACDSD
                     IKRRAYHLRFMLLKSNKLIYKLIFPIITLFGVDITGRILKAIRGNI"
     gene            837..1367
                     /locus_tag="WNA25_000454"
     CDS             837..1367
                     /locus_tag="WNA25_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFA9823287.1"
                     /translation="MNAIRTFQNRMPEIHETCMIDEACVVIGEVSLAEDVSVWPCAVL
                     RGDVNSITVGARSNIQDGSVLHVSHKTAAKPEGSPLVIGEDVTVGHKVMLHGCRIGNR
                     VLVGMGSTVLDDAVIEDDVMIGAGSLVPPRKRLEGGYLYIGSPVRQVRLLTDEEKAFL
                     KYSAAHYVKLSKQYGM"
     gene            complement(1535..3130)
                     /locus_tag="WNA25_000455"
     CDS             complement(1535..3130)
                     /locus_tag="WNA25_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFA9823288.1"
                     /translation="MSQEILDQVRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQSAGT
                     VKGKKTGKFATSDWMDIEKQRGISVASSVMQFDYKDHTVNLLDTPGHQDFSEDTYRVL
                     TAVDSALMVIDAAKGVEAQTIKLLNVCRLRDTPIVTFMNKYDREVRDSLELLDEVEDI
                     LQIRCAPVTWPIGMGKNFKGVYHILNDEIYLFEAGGERLPHEFGIIKGINNPELEQRF
                     PLEIQQLRDEIELVQAASNEFNLDEFLAGELTPVFFGSAINNFGIQEILNSLIDWAPA
                     PKPRDATMRMVGPDEPKFSGFIFKIQANMDPKHRDRIAFLRVCSGKFERGMKMKHLRI
                     NREIAASSVVTFMSHNRELAEEAYAGDIIGIPNHGNIQIGDSFSEGEQLAFTGIPFFA
                     PELFRSVRIKNPLKIKQLQKGLQQLGEEGAVQVFKPMSGADLILGAVGVLQFEVVTSR
                     LANEYGVEAVFDSASIWSARWVSCDDKKKLAEFEKANAGNLAIDAGGNLAYLAPNRVN
                     LGLTQERWPDIVFHETREHSVKL"
     gene            complement(3237..3632)
                     /gene="hisI"
                     /locus_tag="WNA25_000456"
     CDS             complement(3237..3632)
                     /gene="hisI"
                     /locus_tag="WNA25_000456"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HFA9823289.1"
                     /translation="MDKNLLEAVKFDEKGLVCAIAQDAETKRVLMVAWMNAEALQKTV
                     ETGFAHYYSRSRQKQWMKGEESGHTQKVRELRLDCDGDTIVMLIAQNGGIACHTGRES
                     CFYKKWNGGAWETADAVLKDEKEIYGSTH"
     gene            complement(3663..4430)
                     /gene="hisF"
                     /locus_tag="WNA25_000457"
     CDS             complement(3663..4430)
                     /gene="hisF"
                     /locus_tag="WNA25_000457"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFA9823290.1"
                     /translation="MALAKRIIPCLDVKDGRVVKGVNFIGLRDAGDPVEAAKRYNGEG
                     ADELTFLDITASSDNRDTILHIIEEVAGQVFIPLTVGGGVRTVADIRRLLNAGADKVS
                     INTAAVTRPDLINEAAGFFGSQAIVAAVDAKAVNPENTRWEIFTHGGRNPTGLDAVEW
                     AVEMQKRGAGEILLTGMDRDGTKQGFNLPLTRAVAEAVDIPVIASGGVGNVRHLIEGI
                     TEGKADAVLAAGIFHFGEIAIREAKRTMREAGIEVRL"
     gene            complement(4443..5180)
                     /gene="hisA"
                     /locus_tag="WNA25_000458"
     CDS             complement(4443..5180)
                     /gene="hisA"
                     /locus_tag="WNA25_000458"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFA9823291.1"
                     /translation="MLLIPAIDLKEGRCVRLKQGLMEEATVFSDSPADTALHWFEQGA
                     RRLHLVDLNGAFAGVPQNLPAIKDILAAVAKDIPVQLGGGMRDLKTIGQYLDLGLNDV
                     IIGTAAVKNPDLVREACKAFPGRIIVGLDAKDGMAAIDGWATVTGHHVIDLAKRFEDD
                     GVNSIIYTDIGRDGMMSGVNIDATVKLAQSVRIPVIASGGLTGLDDIPALCAAEKHGV
                     AGAITGRAIYEGSIDFAQAQQLADSLD"
     gene            complement(5213..5851)
                     /gene="hisH"
                     /locus_tag="WNA25_000459"
     CDS             complement(5213..5851)
                     /gene="hisH"
                     /locus_tag="WNA25_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFA9823292.1"
                     /translation="MQTAIIDYGMGNLHSVLKSVRTAGQLAGKNTKIFLSGDPDRVSR
                     ADKVIFPGQGAMPDCMAALTRGGLDEAVKDALKNKPFFGICVGAQLLFDHSEEGNTDG
                     LGWFGGKVRRFARDLRDPQGCRLKVPHMGWNTVRQTQNHPLFQGIPQNTRFYFVHSYY
                     FAPENPETILGESDYPSPFACIVGKDNVFATQFHTEKSHDAGLTMLKNFLNW"
     gene            complement(5981..7483)
                     /gene="pta"
                     /locus_tag="WNA25_000460"
     CDS             complement(5981..7483)
                     /gene="pta"
                     /locus_tag="WNA25_000460"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFA9823293.1"
                     /translation="MAKVLVVPVSAGLNTSAAAQAFAKALDAQVFQAVDATAETLLAQ
                     GKSDDWFDALVGKVAALDAANLVIEGIAPDADKIYLAGKNVELALSLDAAAVFAVRSD
                     NTDADALAHQLNLAKQFFAAAPGVLEGFVVDGAAASVAEAAAEKTGLTFFGSSDALKD
                     VSVLAGREAKRLSPAQFRYNLIDFARQAGKRIVLPEGAEPRTVQAAAICHEKGIARCV
                     LLAKREEVEAVAKERGISLPDSLEIIDPASLVEQYVEPMCELRKSKGLTPEDARKQLQ
                     DTVVLGTMMMAQNDVDGLVSGAVHTTANTIRPALQLIKTAPGASLVSSVFFMLLPNQV
                     LVFGDCAVNPNPTAQQLADIAIQSADSAKAFGIDPKVAMISYSTVNSGSGPDVDTVIE
                     ATKLAREKRPDLAIDGPLQYDAATVPGVGKSKAPGSPVAGQATVLVFPDLNTGNCTYK
                     AVQRSANVLSVGPLLQGLRKPVNDLSRGALVEDIVFTIALTAVQAKQMEG"
     gene            complement(7668..8726)
                     /gene="fbpC"
                     /locus_tag="WNA25_000461"
     CDS             complement(7668..8726)
                     /gene="fbpC"
                     /locus_tag="WNA25_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein FbpC"
                     /protein_id="HFA9823294.1"
                     /translation="MTAALHIGHLSKSFQNTPVLNDISLSLDPGEILFIIGASGCGKT
                     TLLRCLAGFEQPDSGEISLSGKTIFSKNTNLPVRERRLGYLVQEGVLFPHLTVYRNIA
                     YGLGNGKGRTAQERQRIEAMLELTGISELAGRYPHELSGGQQQRVALARALAPDPELI
                     LLDEPFSALDEQLRRQIREDMIAALRANGKSAVFVSHDREEALQYADRIAVMKQGRIL
                     QTASPHELYRQPADLDAALFIGEGIVFPAALNADGTADCRLGRLPVQSGAPAGTRGTL
                     LIRPEQFSLHPHSAPAASIHAVVLKTTPKARHTEISLRAGQTVLTLNLPSAPTLSDGI
                     SAVLHLDGPALFFPGNTL"
     gene            complement(8747..10264)
                     /gene="fbpB"
                     /locus_tag="WNA25_000462"
     CDS             complement(8747..10264)
                     /gene="fbpB"
                     /locus_tag="WNA25_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease FbpB"
                     /protein_id="HFA9823295.1"
                     /translation="MSPKKIPIWLTGLILLIALPLTLPFLYVAMRSWQVGINRAVELL
                     FRPRMWDLLSNTLTMMAGVTLISIVLGIACALLFQRYRFFGKTFFQTAITLPLCIPAF
                     VSCFTWISLTFRVEGFWGTVMIMSLSSFPLAYLPVEAALKRISLSYEEVSLSLGKSRL
                     QTFFSAILPQLKPAIGSSVLLIALHMLVEFGAVSILNYPTFTTAIFQEYEMSYNNNTA
                     ALLSAVLTAVCGIVVFGESIFRGKAKIYHSGKGVARPYPVKTLKLPGQIGAIVFLSSL
                     LILGIIIPFGVLIHWMMVGTSGTFALVSVFDAFIRSLSVSALGAILTILCALPLVWAS
                     VRYRNFLTVWIDRLPFLLHAVPGLVIALSLVYFSINYTPAVYQTFIVVILAYFMLYLP
                     MAQTTLRTSLEQLPKGMEQVGATLGRGHFFIFRTLVLPSILPGITAAFALVFLKLMKE
                     LTATLLLTADDVHTLSTAVWEYTSDAQYAAAAPYALMLVLFSGIPVFLLKKYAFK"
     gene            complement(10332..11327)
                     /gene="fbpA"
                     /locus_tag="WNA25_000463"
     CDS             complement(10332..11327)
                     /gene="fbpA"
                     /locus_tag="WNA25_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     FbpA"
                     /protein_id="HFA9823296.1"
                     /translation="MKTSIRYALLAAALTAATPALADITVYNGQHKEAAQAVADAFTR
                     ATGIKVKLNSAKGDQLAGQIKEEGSRSPADVFYSEQIPALATLSAANLLEPLPASTIN
                     ETRGKGVPVAAKKDWVALSGRSRVVVYDTRKLSEKDLEKSVLNYATPKWKNRIGYVPT
                     SGAFLEQIVAIVKLKGEAAALKWLKGLKEYGKPYAKNSVALQAVENGEIDAALINNYY
                     WHAFAREKGVQNVHTRLNFVRHRDPGALVTYSGAAVLKSSQNKDEAKKFVAFLAGKEG
                     QRALTAVRAEYPLNPHVVSTFNLEPIAKLEAPQVSATTVSEKEHATRLLEQAGMK"
     gene            complement(11718..12059)
                     /locus_tag="WNA25_000464"
     CDS             complement(11718..12059)
                     /locus_tag="WNA25_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4375 domain-containing protein"
                     /protein_id="HFA9823297.1"
                     /translation="MTDTDTQADRFEQMMRQAVDKLFEQHDGKLESMDGREQELVLIW
                     RAEADIGNGGILQFVCNWGFPAAEKTCSVLKKIGAVHSAMLIHRAADALGKEIRHLQS
                     EGKNLKEMWDI"
     gene            complement(12117..13493)
                     /gene="argH"
                     /locus_tag="WNA25_000465"
     CDS             complement(12117..13493)
                     /gene="argH"
                     /locus_tag="WNA25_000465"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFA9823298.1"
                     /translation="MHDKTWSGRFNEPVSELVKQYTASIGFDQRLAEWDIQGSLAHAQ
                     MLTRSGVLSENDLTDIRRGMSEILEEIRSGKIEWPLDLEDVHMNIERRLTDKIGDAGK
                     RLHTGRSRNDQVATDIRLWLRDQITVIQNLIQNLQTALLDLAEQNAETVMPGFTHLQV
                     AQPVSFGHHMLAYVEMLGRDFERMADCRKRVNRMPLGAAALAGTTYPIQREITAELLG
                     FEQICQNSLDAVSDRDFAIEFTAAASLVMVHLSRLSEELILWMSPRFGFIDIADRFCT
                     GSSIMPQKKNPDVPELVRGKSGRVIGHLIGLITLMKSQPLAYNKDNQEDKEPLFDTAD
                     TLIDTLRIYADMMRGVTVKPGNMRAAVMQGFATATDLADYLVKKGMPFRDAHEVVAQA
                     VRHADEAGVDLSELPLEALQGFSKLISDDVYGVLTPEGSLNARNHLGGTAPEQVRLQV
                     KRWREMSA"
     gene            complement(13512..14381)
                     /gene="galU"
                     /locus_tag="WNA25_000466"
     CDS             complement(13512..14381)
                     /gene="galU"
                     /locus_tag="WNA25_000466"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFA9823299.1"
                     /translation="MKPIKKAVFPVAGMGTRFLPATKANPKEMLPIVDKPLIQYAVEE
                     AVEAGCTEMVFVTGRNKRSIEDHFDKAYELETKLEMRHKDKLLEHVRNILPPNITCLY
                     IRQAEALGLGHAVLCARAAIGDEPFAVILADDLIDAPKGALKQMVEVYGRSGNSILGV
                     ETVEPSQTGSYGIVETEQLKQFQRITGIVEKPKPEDAPSNLAVVGRYILTPRIFDLLT
                     GLPRGAGNEIQLTDGIAKLLDHEFVLAHPFEGTRYDCGSKLGYLEATVAYGLKHPETG
                     EPFRRLLEKYRTE"
     gene            complement(14409..15008)
                     /gene="rdgB"
                     /locus_tag="WNA25_000467"
     CDS             complement(14409..15008)
                     /gene="rdgB"
                     /locus_tag="WNA25_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HFA9823300.1"
                     /translation="MSEKPEKIVLASGNAGKLKEFGNLFKPYGITVLPQSPFGIPECP
                     EPYPTFVENALAKARHAAGHSGLPALADDSGICAAALNGAPGIHSARYAGSNPKSDTA
                     NNLKLAAELAGKADKSCSYVCVLVFVRHKDDPRPIIAEGIWHGQWHDTPLGQNGFGYD
                     PYFYLPEHGKTAAELDSEVKNRESHRAQALADLIRKLAL"
     gene            complement(15001..15234)
                     /locus_tag="WNA25_000468"
     CDS             complement(15001..15234)
                     /locus_tag="WNA25_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823301.1"
                     /translation="MNRRKIYLLSVALFTLAFMLPVLLGAYLLTAGSKAFAVASFLFA
                     FGALFGQIGALALYLRHKSLRAAPSSSQGNRYV"
     gene            complement(15342..15875)
                     /locus_tag="WNA25_000469"
     CDS             complement(15342..15875)
                     /locus_tag="WNA25_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFA9823302.1"
                     /translation="MADFNQILTPGDVDGGIINVVNEIPAGSNHKIEWNRKLAAFQLD
                     RIEPAIFAKPTNYGFIPQTLDEDGDELDVLLVTEQPLATGVFLEARVIGVMKFVDDGE
                     VDDKIVCVPADDRNNGNAYKTLSDLPQQLIKQIEFHFNHYKDLKKAGTTKVESWGGAE
                     EAKKVIKESIERWNKQA"
     gene            complement(15977..16435)
                     /gene="nudB"
                     /locus_tag="WNA25_000470"
     CDS             complement(15977..16435)
                     /gene="nudB"
                     /locus_tag="WNA25_000470"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFA9823303.1"
                     /translation="MPMAKPLKYPVSALVVLYSGDGGILLIERTHPKGFWQSVTGSLE
                     PGETVAQTARREVWEETGILLEDGQLQDRHDSTVYEIYHHWRHRYPKGVFENREHVFR
                     AEIPRDTPVVLQPEEHVSYGWFGLEEAAEKVFSPSNRRAILELGRFLGKR"
     gene            16646..16722
                     /locus_tag="WNA25_000471"
     tRNA            16646..16722
                     /locus_tag="WNA25_000471"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16680..16682,aa:Pro,seq:ggg)
     gene            16985..18292
                     /gene="mafB"
                     /locus_tag="WNA25_000472"
                     /pseudo
     CDS             16985..18292
                     /gene="mafB"
                     /locus_tag="WNA25_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 2"
     gene            18334..18870
                     /locus_tag="WNA25_000473"
     CDS             18334..18870
                     /locus_tag="WNA25_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EndoU domain-containing protein"
                     /protein_id="HFA9823304.1"
                     /translation="MFDAKPRWEVDRKLNKLTTREQVEKNVQETRRRSQSSQFKAHAQ
                     REWENKTGLDFNHFIGGDINKKGTVTGGHSLTRGDVRVIQQTSAPDKHGVYQATVEIK
                     KPDGSWEVKTKKGGKVMTKHTMFPKDWDEARIRAEVTSAWESRIMLKDNKWQGTSKSG
                     IKIEGFTEPNRTAYPIYE"
     gene            18901..19320
                     /locus_tag="WNA25_000474"
     CDS             18901..19320
                     /locus_tag="WNA25_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823305.1"
                     /translation="MKRIKCFCDKFPSGDTFRMCIILDDYDNRVDYYVGIYDYITSTL
                     MSDIYYRSTIDEHFKIIELIENNPNEIYDDGGGQQFCLEFHHDKVIFYHNEFDEEDGY
                     PVLSCSLHTFKTALIAWNAFLQLPKSIHSVVETVIEE"
     gene            19398..19481
                     /locus_tag="WNA25_000475"
                     /pseudo
     CDS             19398..19481
                     /locus_tag="WNA25_000475"
                     /inference="COORDINATES: protein motif:HMM:NF026862.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            19478..19720
                     /locus_tag="WNA25_000476"
     CDS             19478..19720
                     /locus_tag="WNA25_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein 31"
                     /protein_id="HFA9823306.1"
                     /translation="MTNLKLDFYSEVIIKDSCPNDLLENGETIKGKKGVVLGISEEDG
                     IIYGYTILLFDIKYCIYIDKKYIIPTGKKFSRDDFY"
     gene            19798..20043
                     /locus_tag="WNA25_000477"
     CDS             19798..20043
                     /locus_tag="WNA25_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFA9823307.1"
                     /translation="MKDSTGQMRLATKDLAEAIKRGEVRSSAFTTKQLKAIEKGKDKI
                     PSYTWHHHQDTGRMQLVPEWEHSKTGHIGGTAMGKGK"
     gene            20046..20432
                     /locus_tag="WNA25_000478"
     CDS             20046..20432
                     /locus_tag="WNA25_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823308.1"
                     /translation="MWKIYKENSTDLNFAIGSIYCQAINLTEFKMWVEKIIREVDLDE
                     IPNYFFDLIDFQSLYDLIDIIGFVPENNLSKNQDNALTGIAFLRGIDVYDPPISKEKA
                     LKALEKHPEIYQKFQHFFPFVELPPL"
     gene            20507..20650
                     /locus_tag="WNA25_000479"
     CDS             20507..20650
                     /locus_tag="WNA25_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFA9823309.1"
                     /translation="MFDGKGTSEIPNYTWHHHQDTGRMQLIREDSHHDTDHIGWRAMS
                     KGK"
     gene            20653..21043
                     /locus_tag="WNA25_000480"
                     /pseudo
     CDS             20653..21043
                     /locus_tag="WNA25_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21083..22540)
                     /locus_tag="WNA25_000481"
     CDS             complement(21083..22540)
                     /locus_tag="WNA25_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFA9823310.1"
                     /translation="MHKILPIAHVLSRLGMLFSFILLIPAALSYAFSDGAYTAFATTA
                     TVTLSGSCIVRLATLRFRRELRPRDGFTLVLMLWLAFAAMAAMPMYLYFPNMGFTDAF
                     FESMSGLTTTGATVIPHVDGLAPSVNFWRHMLNWLGGMGIIVLAVAILPMLGVGGTQL
                     FKAEIPGIDKESKMSPRISQVAKKLWFGYTLITILAAACLHFAGMGWFDAVCHALATL
                     SLGGFSTHDASIGYYNSPLIEAVIIVFTIFGGINFASHFAALNSRSLKTYWKDEECRT
                     MLLLLSGSILAAALYLWHTGHYAGFGESLRYTAFNFVSIGLANGLSNTDFAQWPLLIS
                     LWMFFLANILASSGSTGGGIKTIRALVLFKFSLREMMVLLHPKAVRTVKISGKAIPDR
                     LALTVMSFIFIYFMTVVLFSFLLMASGMEFTTAFTAVIACITNAGPGLGEVGPAGNYA
                     GLDVMQKWICVTAMLLGRLEIFTVFILFTSAYWKK"
     gene            complement(22555..23385)
                     /locus_tag="WNA25_000482"
     CDS             complement(22555..23385)
                     /locus_tag="WNA25_000482"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HFA9823311.1"
                     /translation="MAHYAIGDIQGCFDELTALLGKIGFNHGTDTLWLTGDIVNRGPK
                     SLETLQFCIRHENSVQIVLGNHDLYLLAVGCGEGALKRSDTIEPILKHPDGGKMLDWL
                     RAQPLLIREGGRVMIHAGILPQWRIAKAESLAGEAEAELRGKKYVKFFSKMYGNKPAA
                     WDEGLEGYARLRFIVNAFTRMRALTFKNELDFDYKSTVKKMPPYLRPWFKAPDRQNLD
                     HTIIFGHWSSLGYTNADNVISLDTGALWGGQLTAVNLETEEITQVQAAGGIDWKSFAK
                     "
     gene            complement(23462..23851)
                     /locus_tag="WNA25_000483"
     CDS             complement(23462..23851)
                     /locus_tag="WNA25_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFA9823312.1"
                     /translation="MSKTVIHTDKAPAAIGAYSQAVRAGGTVYMSGQIPLDPATMTVV
                     GNGDFHTEARQVFQNLQAVAEAAGGSLDDIVKLNAYLTDLGNFAVFNEVMAEFITEPF
                     PARAAVGVASLPKGVQVEAEAVLVLNA"
     gene            complement(23998..24525)
                     /gene="nspA"
                     /locus_tag="WNA25_000484"
     CDS             complement(23998..24525)
                     /gene="nspA"
                     /locus_tag="WNA25_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein NspA"
                     /protein_id="HFA9823313.1"
                     /translation="MKKALAALIALALPAAALAEGASGFYVQADAAHAKASSSLGSAK
                     GFSPRISAGYRINDLRFAVDYTRYKNYKQAPSTDFKLYSIGASVIYDFDTQSPVKPYF
                     GARLSLNRASAHLGGSDSFSKTSAGLGVLAGVSYAVTPNVDLDAGYRYNYIGKVNNVK
                     NVRSGELSAGVRVKF"
     gene            complement(24909..26084)
                     /locus_tag="WNA25_000485"
     CDS             complement(24909..26084)
                     /locus_tag="WNA25_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase-like protein"
                     /protein_id="HFA9823314.1"
                     /translation="MHTISFPNRTRLTALPPLSLYIHIPWCIKKCPYCDFNSHSLKNG
                     LPEAAYIDALLTDLQLELPNIWGRPVETIFFGGGTPSLFQAESIDRLLSGVRSLLRLQ
                     PEAEITLEANPGTFEIEKFQGFKDAGITRLSIGVQSFNDDMLSRLGRVHNGKEALTAI
                     DTALKLFDKVNIDLMYSLPNQTVQTALDDVQTAIATDATHISAYHLTMEPNTPFGHTP
                     PKGLPQDEAALDIEDAVHGTLEGAGFIHYETSAFAKPTMQCRHNLNYWQFGDYLGIGA
                     GAHGKISYPDRIERTVRRRHPNDYLALMQSQPGEAVERKTVAAEDLPFEFMMNALRLT
                     DGVPAAMLQERTGVPAAKIMVQIETARQKGLLETDPTVFRPTEKGRLFLNDLLQCFL"
     gene            complement(26141..28612)
                     /gene="ligA"
                     /locus_tag="WNA25_000486"
     CDS             complement(26141..28612)
                     /gene="ligA"
                     /locus_tag="WNA25_000486"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFA9823315.1"
                     /translation="MNPTAQRIHELTDLLNRYAYEYYTLDAPSIPDAEYDRLFRELEA
                     LERNHPELKLPDSPTQRVGGEPLAGFAEVRHEVPMLSLTNAFSPQDENGVFDHAEMYA
                     FDQRVRDGLDGGNPEYVIEPKFDGLAISLLYRDGVLVQAATRGDGTTGEDVTRNVKTV
                     SNIPLRLHGENVPELIEVRGEVLMLKADFAGLNKRQAENGQKPFANPRNAAAGSLRQL
                     DSRITAQRKLHFFPYSIARQQGGFEAEEHIQELAYFQELGFSLPNGNFGCFKNIGEVL
                     AFYEHMQQKRPELPYEIDGMVVKVNSLAQQRELGFISRAPRWAVAHKFPAEEALTIVE
                     AIDVQIGRTGAVTPVARLQPVFVGGVTVTNATLHNQDEVSRKDVRVGDTVVVRRAGDV
                     IPEVVRVIFERRPMQETAVAVSDGIGHQQDDLFAETPSAKQTESVPLHKPYRLPARCP
                     ICRSEIEREEGEAVARCSGGMLCQAQRAQGLIHFASRKAMDIDGLGEKQIEQLVAQDL
                     VRHFADLYRIDIPTLQKMKETADKGSSENENGDAETVSGDLSKYNTQNGKKQPTKWAQ
                     NILAGIESGKTPELARFLFALGIRHVGERTAKTLAQAFGTLERVRRAPEPVLACLPDI
                     GTVVARSIAHFFAQAEQQAMIDELLAAGVAPQAQAVSLPAAQYAGPQRWITRLPGFKI
                     SENKAQALWELAGQSIEGLQNDKALPADWQAWRSKAQNTALLENLKTFFAQMPSEDEA
                     AQGSDGINKAVAGKTFVLTGTLPTFKRDQAQALIEAAGGKVSGSVSKKTDYVVAGEAA
                     GSKLEKANALGVSVLSEAELLTLLC"
     gene            complement(28746..30032)
                     /locus_tag="WNA25_000487"
     CDS             complement(28746..30032)
                     /locus_tag="WNA25_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFA9823316.1"
                     /translation="MIYIVLFLAAVLAVVAYNMYQENQYRKKVRDQFGHSDKDALLNS
                     KTSHVRDGKPSGGPVMMPKPQPAVKKPAKPQDSAMRNLQEQDAVYIAKQKQAKASPFK
                     TEIETALEEIGIIGNSAHTVSEPQTGHSAPKPADAPAKPVPVPQTPAKPLITLKELSK
                     VELPWFDVRFDFISYIALTEAKELHALPRLSNRCRYQIVGCTMDDHFQIAEPIPGIRY
                     QAFIVGIQAVSRNGLASQEELSAFNRQADAFAQSMGGQTLHTDLAAFIEVASALDAFC
                     ARVDQTIAIHLVSPTSISGVELRSAVTGVGFVLEDDGAFHYTDTSGSTMFSICSLNNE
                     PFTNALLDNQSYKGFSMLLDIPHSPAGEKTFDDLFMDLAVRLSGQLNLNLVNDKMEEV
                     STQWLKDVRTYVLARQSEMLKVGIEPGGKTALRLFS"
     gene            complement(30226..30798)
                     /gene="ampD"
                     /locus_tag="WNA25_000488"
     CDS             complement(30226..30798)
                     /gene="ampD"
                     /locus_tag="WNA25_000488"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFA9823317.1"
                     /translation="MDNHAGAHWQNGWLQSIRHTPSPNFSPRETGETVSLIVLHNISL
                     PPFEYGTDAVEKLFANRLDPDGHPFFSLIHTLHVSSHFLIARDGETVQFVSCGDMAYH
                     AGASSFRGREKCNAFSIGIELEGCDFEPFAEAQYRSLETLLAALCRRYPITAVTGHQD
                     IAPGRKTDPGHFFDWRRIREKGFPVDRNAV"
     gene            30882..31877
                     /gene="mltG"
                     /locus_tag="WNA25_000489"
     CDS             30882..31877
                     /gene="mltG"
                     /locus_tag="WNA25_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFA9823318.1"
                     /translation="MLKKMLKWTAVFLTVSAAVFAALLFVPKDNGRAYRIKIAKNQGI
                     SSVGRKLAEDRIVFSRHVLTAAAYVLGVHNRLHTGTYRLPSEVSAWDILQKMRGGRPD
                     SVTVQIIEGSRFSHMRKVIDATPDIGHDTKGWSNEKLMAEVAPDAFSGNPEGQFFPDS
                     YEIDAGGSDLQIYQTAYKAMQRRLNEAWAGRQDGLPYKNPYEMLIMASLIEKETGHEA
                     DRDHVASVFVNRLKIGMRLQTDPSVIYGMGAAYKGKIRKADLRRDTPYNTYTRGGLPP
                     TPIALPGKAALDAAAHPSGEKYLYFVSKMDGTGLSQFSHDLTEHNAAVRKYILKK"
     gene            31937..32557
                     /locus_tag="WNA25_000490"
     CDS             31937..32557
                     /locus_tag="WNA25_000490"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFA9823319.1"
                     /translation="MKPQFITLDGIDGAGKSTNLAVIKAWFERRGLPVLFTREPGGTP
                     VGEALREILLNPETKAGLRAETLMMFAARMQHIEEVILPALSDGIHVVSDRFTDATFA
                     YQGGGRGMPSEDIEILEHWVQGGLRPDLTLLLDVPLEVSMARIGQAREKDRFEQEQAD
                     FFMRVRGVYLDRAAACPERYAVIDSNRSLDEVRNSIEKVLDGHFGC"
     gene            32779..34059
                     /locus_tag="WNA25_000491"
     CDS             32779..34059
                     /locus_tag="WNA25_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme"
                     /protein_id="HFA9823320.1"
                     /translation="MENSLKEAALKFHELPVPGKISVTPTKSLATDKDLALAYSPGVA
                     APCMEIHADPQNAYKYTGKGNLVAVISNGTAVLGLGDIGALAGKPVMEGKGVLFKKFA
                     GVDVFDIEIDEKDPQKLVDIIAALEPTFGGINLEDIKAPECFYIERELRKRCKIPVFH
                     DDQHGTAIITAAAVLNALRYTGRKIEEATLVCSGAGAAAIACLNQLLDLGLKRENVTV
                     CDSKGVIYQTREDKDRMDESKKFYAVEDNGRRVLADAVKGKDIFLGLSGANLLTPEML
                     NTMNEKPIVFAMANPNPEILPPLAKETRPDVVIGTGRSDFPNQVNNVLCFPFIFRGAL
                     DVGATTINEEMKRACVYALADLAMEEVTEEVVAAYGKKFEFGAEYLIPTPFDSRLLPR
                     VATAAAKAAMESGVATRPIADLEAYAAKLGEWKL"
     gene            34466..35497
                     /locus_tag="WNA25_000492"
     CDS             34466..35497
                     /locus_tag="WNA25_000492"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025462052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFA9823321.1"
                     /translation="MPNFFKFHTVIERHWQKPYPVLSFLLKPLSGLFAKIAAKWRADF
                     LSGKRQSEKLSVPVVVVGNIHAGGTGKTPIAAALVSGLQEKGVKVGIISRGYGRKSKA
                     VYVLNAASRAEDAGDEPLLLFRKTGAPTAVGSSRVEAGRALLAAHPELELIVADDGLQ
                     HYALQRDVEIAVFPAADTGRTDLDLLPNGNLREPLSRLESVDAVVVGGRAADGFMPSE
                     HLFGSRIEAGAVYRLNRPSEKLDISTLSGKRVAAVAGIARPQRFFDTLTHMGIRLDQT
                     VALPDHADIFNRDLPPADVVLVTEKDAVKFSDGICTDNVWVLPVCAIIEPDLAEFVLE
                     RLEGVPKAV"
     gene            35703..36236
                     /locus_tag="WNA25_000493"
     CDS             35703..36236
                     /locus_tag="WNA25_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HFA9823322.1"
                     /translation="MKLKTLLLPFAALALCANAFAAPPGDASLARWLDTQNFDRDIEK
                     NMIEGFNAGFKPYADKALAEMPEAKKDQAAEAFNRYRENVLKDLITPEVKQAVRNTLL
                     KNAREIYTQEEIDGMIAFYGSPVGQSVVAKNPRLIKKSMSEIAVSWTALSGKIARHHL
                     PEFTEELRRIICGGIVD"
     gene            36221..36328
                     /locus_tag="WNA25_000494"
                     /pseudo
     CDS             36221..36328
                     /locus_tag="WNA25_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            36458..36640
                     /locus_tag="WNA25_000495"
     CDS             36458..36640
                     /locus_tag="WNA25_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HFA9823323.1"
                     /translation="MEKKFLDILVCPVTKGRLEYHQDKQELWSRQAKLAYPIKDGIPY
                     MLENEARALSEEELKA"
     gene            36637..37398
                     /gene="kdsB"
                     /locus_tag="WNA25_000496"
     CDS             36637..37398
                     /gene="kdsB"
                     /locus_tag="WNA25_000496"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFA9823324.1"
                     /translation="MTEFVVLIPARLDSSRLPGKALADIHGKPMVVRVAEQAAKSKAA
                     RVVVATDHPDIQTACQAHGIEVVMTSNRHESGTTRLAEAAAALKLPPHLIVVNVQGDE
                     PLIAPELIDRTAEVLVENNVQMATAGHELHDFDELMNPNAVKVVLDKNGNAIYFSRAP
                     IPYPRDAMRAGKREMPSETAVLRHIGIYAYRVGFLQRYAEMSVSPLETIESLEQLRVL
                     WHGYPIAVETAKEAPAAGVDTQEDLDRVRAVFQTV"
     gene            37421..37717
                     /locus_tag="WNA25_000497"
     CDS             37421..37717
                     /locus_tag="WNA25_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFA9823325.1"
                     /translation="MQQHIEKWQHLSREEQKILAEVWGLVQNDDQEVHYEMLKLNAPD
                     EVSGEFWFRMAETLSTLPPNRSLDLRMNGGRLSTAVSILSVMIEDNPDIPQLWA"
     gene            37739..37936
                     /locus_tag="WNA25_000498"
     CDS             37739..37936
                     /locus_tag="WNA25_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823326.1"
                     /translation="MVHGHKARADGLVQQPDKAAEANEEEYLTKALSQNLLSTLDAAL
                     ARFPEDAWFQEIKQDAQKHFA"
     gene            38093..38185
                     /locus_tag="WNA25_000499"
     tRNA            38093..38185
                     /locus_tag="WNA25_000499"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:38127..38129,aa:Ser,seq:gct)
     gene            38459..39244
                     /locus_tag="WNA25_000500"
     CDS             38459..39244
                     /locus_tag="WNA25_000500"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFA9823327.1"
                     /translation="MSRIRQAFAALDGGKALIPYIAVGDPDIRTTLALMHGMVASGAD
                     ILELGVPFSDPMADGPVIQRAAERALANGISLRDVLDVVRKFRETDTQTPVVLMGYLN
                     PIHKMGYREFAQEAAKAGVDGVLTVDSPIETIDSLYRELKDNEVDCIFLIAPTTTEDR
                     IKTIAELAGGFVYYVSLKGVTGAASLDTDEVSRKIEYLRQYIDIPIGVGFGISNAESA
                     RKIGRVAAAIIVGSRIVKEIENNAGNEAAAVGALVKELKDAVR"
     gene            39281..40153
                     /locus_tag="WNA25_000501"
     CDS             39281..40153
                     /locus_tag="WNA25_000501"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HFA9823328.1"
                     /translation="MSWLDKILPPKIKNRGKDGSSNVPEGLWRKCPSCSATVYSTELQ
                     QNNQVCPKCNHHNPLSARQRLNLLLDEEGREEVAGNVKPTDPLKFKDSKKYPDRLSAA
                     RKLTGEDDALVVMKGMMNGLPVVVAAFEFRFIGGSMGSVVGERFVQGVRRAVADNCPF
                     VCVAASGGARMQEGVNSLMQMTKTSAALHLLTEKRLPFISVLTDPTMGGVSASFAFLG
                     DVVLAEPNALIGFAGPRVIEQTVRETLPEGFQRAEFLLEKGAIDQIVDRRDMKRRISD
                     LITLLCRQDKVSAT"
     gene            complement(40213..40824)
                     /locus_tag="WNA25_000502"
     CDS             complement(40213..40824)
                     /locus_tag="WNA25_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HFA9823329.1"
                     /translation="MRRAILLILTLTVGTSLAAGFSQKDTPINTRYRETPEEAAAREF
                     KEHTAELPPLPDAHSDGWFDIYVDENYGKQPKILLDSLQIMPAPDGSIRYILNIRSDK
                     GYDNLTAEGIFCALSSIGFGNGKLSSYKVFGYGDTVNSRWIQPRNAEWKPIGGTLGRN
                     DALRAVLYQAFCEGGVPADTQGLVQRLKERAGRYAPSMKPHDK"
     gene            40932..41156
                     /locus_tag="WNA25_000503"
     CDS             40932..41156
                     /locus_tag="WNA25_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HFA9823330.1"
                     /translation="MNSETLDVTGLKCPLPILRAKKALAQMRQGEVLTVLATDGGAPG
                     DFEAFCRQTGHVLLDSSEQDGVFTLVVKHK"
     gene            complement(41190..41528)
                     /locus_tag="WNA25_000504"
     CDS             complement(41190..41528)
                     /locus_tag="WNA25_000504"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823331.1"
                     /translation="MFQKRGTVCPLGFHTATVARQAQIINAAIAFKRPQRLYIGFCNS
                     GRQTGQVFLSDRDKMSDSGKLGNRFETTLSQDAGTPKQIDGYACKILMPYTGLPVLGI
                     TRSKHKCRQQ"
BASE COUNT         9197 a         9899 c        12431 g        10036 t
ORIGIN      
        1 accaaatacc aaatatatat atcaggataa ccaaggattg tcggaggcga gaaataccgg
       61 aataaaaaat tcaaatggaa aatatatagc atttattgat tcggatgatt ttattaattg
      121 tcaggttttg ctggattttc ttggtaaaga tgatagtgat atgccggatg tggttttttt
      181 aaatgcagtt aaatatgata agggaagagt ttcatatttt ggcgaagatt atcagcctga
      241 aaaaatatta aatcaatcca aagtcgaagt tttgaaagga ttgtgccgat ttagaaaatt
      301 tccgggttcg gcgtggaata agattataaa aagagaattg attattagag aaaaactgtt
      361 ttttgaaaag ggaatttatt ctgaagatat tgaatggtca atgaggttgt ttaatgcggc
      421 aacaactttt tcttatttgg acggttgtta ttactattat cggcagggaa gaaaagattc
      481 tattacggga actgtttcgg aaaaaggtat aaagtcatta ttatatattt tggaaaaaaa
      541 tgcgaaaatg gaatttaata gggatatatc gagttatctt tattcttttc tttcctacga
      601 atatctcgtt ttgcttttta taatgacgag taaaaatata gcgtgtgatt ctgatataaa
      661 aagaagggca tatcatttaa ggtttatgct gttaaagtcc aataagctga tatataagct
      721 gatattcccg ataatcacat tattcggggt cgatattaca ggcaggattt taaaagcaat
      781 cagggggaat atttaataaa tcctttaaca atatatacct taccgaagga ggaaaaatga
      841 acgcaatcag aactttccaa aaccgcatgc ccgaaatcca cgaaacctgc atgatagacg
      901 aggcgtgcgt cgtcatcggc gaagtctcgc ttgccgaaga tgtttccgtg tggccgtgcg
      961 ccgtgttgcg cggcgatgtg aacagcatca ccgtcggcgc gcgcagcaat atacaggacg
     1021 gcagcgtcct gcacgtttcc cacaaaaccg ccgccaaacc cgaagggtcg ccgctggtta
     1081 tcggcgaaga cgttaccgtg gggcacaaag tgatgctgca cggctgccga atcggtaacc
     1141 gcgtcttggt cggtatgggc agcaccgttt tggacgatgc cgttattgaa gatgacgtga
     1201 tgatcggcgc gggcagcctc gttccgccgc gcaaacgttt ggagggcggc tatctttata
     1261 tcggttcgcc tgtgaggcag gttcgtctgc tgaccgatga ggaaaaagcc tttttgaaat
     1321 attccgccgc acattatgtg aagctgtcga aacagtacgg gatgtgaaat cacatcggcg
     1381 ttcttgcgtc agtcccaaat tcacgcggtt gggatgcccg ataacggtat ccgatgcgcc
     1441 ttgattttga ccggctgcgt ttgagttgca ggcaaaaatg ccgtctgaaa gccttttttt
     1501 cggcttcgga cggcatttta ttgccgattt ctttttagag tttgaccgaa tgttcgcgcg
     1561 tttcgtggaa cacgatgtcc ggccaacgtt cttgcgtcaa ccccaaattc acgcggttgg
     1621 gggcgaggta ggcgaggttg ccgcctgcgt cgatggcgag gttgcctgcg ttggcttttt
     1681 caaattccgc cagttttttc ttgtcgtcgc acgataccca gcgcgccgac cagatggatg
     1741 cgctgtcgaa cacggcttcc acgccgtatt cgttggcgag gcgtgaggtt acgacttcaa
     1801 actgcaacac gccgaccgca cccaaaatca aatccgcgcc gctcatcggt ttgaatactt
     1861 gaaccgcacc ttcttcgccg agttgttgca aacctttttg cagttgtttg attttcagcg
     1921 ggtttttgat gcggacgctg cggaacagtt cgggtgcgaa gaatgggatg ccggtaaacg
     1981 ccagttgttc gccttcggag aagctgtcgc cgatttggat gttgccgtgg ttcgggatgc
     2041 cgatgatgtc gccggcgtag gcttcttccg ccagttcgcg gttgtgcgac atgaaggtta
     2101 ctacgctgga ggcggcgatt tcgcggttga tacgcaggtg tttcatcttc atgccgcgct
     2161 cgaatttacc ggagcagacg cgcaagaagg cgatacggtc gcggtgtttc gggtccatat
     2221 tggcttggat tttaaagata aatccggaaa atttcggctc gtccggcccg accatgcgca
     2281 tggtcgcgtc gcgcggtttc ggtgcgggtg cccagtcaat caatgaattg aggatttcct
     2341 gaatgccgaa gttgttaatc gcagagccga agaacactgg cgtgagttcg ccggcgagaa
     2401 attcgtcgag attaaattcg ttggaagccg cctgcaccaa ttcgatttcg tcgcgcaact
     2461 gctggatttc caacggaaag cgttgttcca attcgggatt gtttatgcct ttgatgatgc
     2521 cgaactcgtg cggcaggcgt tcgccgcccg cttcaaagag atagatttcg tcgttcagga
     2581 tgtggtacac gcccttgaag tttttgccca taccgatcgg ccaggtaacg ggcgcgcagc
     2641 ggatttgcag gatgtcttcc acttcgtcca agagttccaa agaatcgcgc acttcgcggt
     2701 cgtatttgtt catgaaggta acaatcggcg tatcgcgcag gcggcagacg ttcaagagtt
     2761 tgatggtttg cgcttccacg ccttttgccg cgtcgatgac catcaaggcg ctgtccactg
     2821 cggttaaaac gcggtaggtg tcttcggaga agtcttggtg tcccggcgtg tccaagaggt
     2881 tgacggtgtg gtctttgtag tcgaactgca tcacgcttga tgccacggaa atgccgcgct
     2941 gcttctcgat gtccatccag tcggaggtgg cgaatttgcc ggttttctta cctttcaccg
     3001 tgcctgcgct ttgaatcgcg cccgaaaaca gcagcagttt ttcggtcagc gtggttttac
     3061 ccgcatcggg gtgggagatg atggcaaacg tgcggcggcg gcgcacttgg tcgaggattt
     3121 cttgggacat ggttttcttt gcaaaaaggt tcaggccgct tttcagacgg cccggacagt
     3181 gtttgagacg gcgaaattgt acaaaaaaat gcctgataat tcaatgttgg aggcggtcag
     3241 tgcgtgctgc cgtaaatctc tttttcgtct ttcaggacgg catcggcggt ttcccacgca
     3301 ccgccgttcc attttttgta aaagcagctt tcgcgcccgg tgtggcaggc gatgccgccg
     3361 ttttgggcga tgagcatcac aatggtatcg ccgtcgcagt cgaggcgcag ttcgcggact
     3421 ttttgcgtgt gtcccgactc ttcgcccttc atccattgtt tttggcgcga acggctgtaa
     3481 tagtgggcaa agccggtttc gacggttttt tgcagggctt cggcgttcat ccacgccacc
     3541 attaaaacac gtttggtttc ggcatcttgg gcgatggcgc aaaccaaacc tttttcgtca
     3601 aatttgacgg cttcaagcag gtttttatcc atatttcctt tcagacggca tagtcggggc
     3661 ggtcagaggc gcacttcgat gccggcttcg cgcatagtgc gtttggcttc gcggatggcg
     3721 atttccccga aatggaaaat gccggcggca agtacggcat cggctttgcc ttcggttatg
     3781 ccttcaatca ggtgccggac attgccgacc ccgccggagg cgatgacggg gatgtcgacg
     3841 gcttcggcaa cggcgcgggt cagcggcagg ttgaaaccct gtttcgtccc gtccctgtcc
     3901 ataccggtga gcaggatttc gcccgcgccg cgtttttgca tttcgaccgc ccattccacc
     3961 gcatccaaac cggtcggatt gcgcccgccg tgggtaaaga tttcccagcg tgtgttttcg
     4021 gggttgacgg ctttggcatc gacggcggcg acgatggctt gcgaaccgaa aaatccggcg
     4081 gcttcgttaa ttaaatcggg acgggtaacg gcggcggtgt tgatgctgac tttgtccgcg
     4141 ccggcattga gcaggcggcg gatgtcggca acggtgcgta cgccgccgcc gacggtcagc
     4201 gggatgaaga cttgtccggc aacctcttcg atgatgtgca ggatggtgtc gcggttgtcg
     4261 gatgaggcgg tgatgtcgag gaaggtcaat tcgtccgcgc cttcgccgtt gtagcgtttg
     4321 gcggcttcga cggggtcgcc cgcgtcgcgc aaaccgatga agttcacgcc tttgacgacg
     4381 cgcccgtctt ttacgtcgag acaggggatg atgcgttttg ccagtgccat aatcggatgc
     4441 ctttagtcga gggaatctgc cagttgctgc gcttgggcaa aatcgatgct gccctcgtaa
     4501 atcgcgcggc cggtaatcgc gcctgctacg ccatgttttt cggcggcaca cagggcgggg
     4561 atgtcgtcca agccggtcag tccgccggag gcgatgacgg gaatgcggac ggattgggcg
     4621 agtttgaccg tcgcgtcgat gttcacgccg ctcatcatac cgtcgcgccc gatgtcggtg
     4681 tagatgatgc tgttgacacc gtcgtcttca aagcgttttg ccaaatcaat tacatgatgc
     4741 ccggttacgg ttgcccagcc gtcgatggcg gccataccgt ctttggcatc cagtccgacg
     4801 ataatcctgc cggggaaggc tttgcacgct tcgcgcacca agtcggggtt tttgactgcc
     4861 gccgtgccga taatcacgtc gttcagtccc aaatccaaat attgtccgat ggttttcaaa
     4921 tcgcgcatgc cgccgccgag ctgcacgggg atgtctttgg cgacggcggc gaggatgtct
     4981 ttgatggcgg gcaggttttg cggaacgccg gcaaatgcgc cgttcaaatc taccaggtgc
     5041 aggcggcgcg cgccttgttc gaaccagtgc agcgcggtgt cggcgggcga atcggaaaag
     5101 acggtcgcct cttccatcag cccttgtttc aagcggacgc aacgtccttc tttcaaatcg
     5161 atggcgggta tcagcagcat agtttttctc cttgtgcggg gccgtgtcgg gcttaccagt
     5221 ttaaaaagtt tttcaacatc gtcagcccgg catcgtggct tttttcggtg tgaaattgcg
     5281 tggcgaatac gttgtctttg ccgacgatgc aggcaaacgg ggacgggtag tcgctttcgc
     5341 ccaatatggt ttcgggattt tcgggggcga aatagtagct gtggacgaaa taaaaacgcg
     5401 tgttttgggg gatgccttga aaaagcgggt ggttttgggt ttggcgcacg gtgttccagc
     5461 ccatatgcgg gactttcaga cggcatccct gcgggtcgcg gaggtcgcgg gcaaagcgtc
     5521 tgactttgcc gccgaaccag cccaagccgt cggtgtttcc ttcttcgctg tggtcgaata
     5581 aaagttgcgc accgacgcag attccgaaaa acggtttgtt ttttaaggca tctttgactg
     5641 cctcgtccaa gccgccgcgc gtcagtgccg ccatacagtc gggcatcgcg ccctgcccgg
     5701 gaaaaatgac tttgtcggcg cgggacacgc ggtcggggtc gccgcttaaa aagattttgg
     5761 tattttttcc ggcaagctgc cccgccgtcc ggacggattt caatacggaa tgcaggttgc
     5821 ccataccgta atcgataatg gcggtttgca tggcttcctc ctcttttttg caatatggct
     5881 gcgattttaa caaacaaatg tgccgggctg ataaaaatgc cgtctgaaaa cgggaatctg
     5941 tcttcagacg gcatagggtt caaacccgga aagccgtttg tcagccttcc atttgttttg
     6001 cctgaacggc agtcagggcg atggtgaaca cgatgtcttc caccagcgcg ccgcgagaca
     6061 ggtcgttgac cggtttgcgc aggccttgca gcagcgggcc gacgctcaag acgttggcac
     6121 tgcgttggac ggctttatag gtgcagttgc cggtgttcag gtcggggaag accaaaacgg
     6181 ttgcctgtcc tgccaccggg ctgcccgggg ctttggattt gcctacgccc ggcacggttg
     6241 ccgcgtcata ttgcagcggg ccgtcgatgg cgaggtcggg gcgtttttcc cgggcaagtt
     6301 tggtggcttc gatgacggta tcgacatcgg ggccgctgcc ggagttgacg gtggagtagg
     6361 aaatcatcgc cactttcggg tcgatgccga aggcttttgc ggaatcggca gactggatgg
     6421 cgatgtcggc aagctgttgc gcggtcgggt tcggattaac cgcgcagtcg ccgaacacca
     6481 gaacctgatt tggtagcagc ataaagaata cgctggatac gaggcttgcg cccggtgcgg
     6541 ttttaatcag ttgcaaagcg gggcggatgg tgttggcggt ggtgtgaacc gcaccggata
     6601 ccaaaccgtc cacatcattt tgcgccatca tcatcgtacc gagtaccacg gtgtcttgca
     6661 gttgcttgcg cgcgtcttcg ggtgtcaggc ctttggattt gcgcagttcg cacatcggct
     6721 cgacgtattg ttcgaccaat gaggcgggat cgatgatttc caaagagtcg ggcaggctga
     6781 tgccgcgttc tttggcaacg gcttcgactt cttcgcgttt ggcaagcagg acgcagcggg
     6841 caatgccttt ttcgtggcag atggcggcgg cttggacggt gcggggttct gcgccttcag
     6901 gcaggacgat gcgtttgccg gcttggcggg cgaagtcgat caggttgtag cggaattgcg
     6961 ccggcgacag gcgttttgct tcgcggcctg ccaatacgga tacgtctttc agcgcgtcgc
     7021 tcgaaccgaa gaaggtcagg ccggttttct cggctgccgc ttcggcaacg gaggctgccg
     7081 caccgtccac gacaaaacct tccaatacgc ccggcgcggc ggcgaagaac tgtttggcaa
     7141 ggttcaattg gtgtgccagc gcgtcggcat cggtattgtc ggaacggacg gcgaagacgg
     7201 ctgccgcgtc aagggacaat gccagttcga cgtttttgcc tgcgagatag attttgtcgg
     7261 catcgggcgc gatgccttcg atgacgaggt tggcggcatc gagtgcggca actttgccga
     7321 ccagtgcgtc gaaccagtcg tcgcttttgc cttgcgcgag cagggtttcg gcggttgcgt
     7381 caacggcttg gaaaacttgt gcgtccagtg cttttgcaaa ggcttgtgcg gcggcggagg
     7441 tgttcagtcc ggcagatacg ggtacgacga gtacttttgc cataatatat cctttcgtat
     7501 gctgcggtgt gcggcatatg tggttggaag gggcggcata tgggcagaaa cggctgcctg
     7561 cgtgccgtgc gtgccgtgtt tggcttgggg cgcgcaggtt gaatatagca aacaaattct
     7621 gtttccaaca agataaatat ccgcaggctt gtggatgctg ccgcctttca gagggtattt
     7681 ccggggaaga acagggcggg accgtccaaa tggaggacgg cggaaatgcc gtctgacagg
     7741 gtgggggcgg aagggaggtt gagcgtgagg acggtttgtc cggctctgag gctgatttcg
     7801 gtatgccgcg ctttgggcgt ggttttgaga accacggcgt gaatggaggc ggcgggtgcg
     7861 gaatgggggt gaaggctgaa ctgttccgga cggatgagca gtgtaccgcg cgtgcctgcg
     7921 ggtgcgccgc tttggacggg caggcggccc aatctgcaat cggcggtgcc gtcggcgttg
     7981 agcgcggcgg ggaacacgat gccttcgccg ataaacaggg cggcatcaag gtcggcaggt
     8041 tgtcggtaca attcgtgagg gcttgcggtt tggaggatgc gcccctgttt catcacggca
     8101 atccggtcgg cgtattgcag ggcttcttcg cggtcgtggc tgacaaaaac ggcggatttt
     8161 ccgttggcgc gcagggcggc aatcatgtct tcgcgaatct ggcggcgcaa ctgttcgtcc
     8221 agcgcgctga agggttcgtc caacaaaatc agttcggggt cgggggcgag ggcgcgggcg
     8281 agggcgacgc gctgttgttg tccgcccgaa agttcgtgcg gatagcgtcc ggcaagttcg
     8341 gaaatgccgg tcaattccaa catggcttcg atgcgctgtc gctcttgcgc cgtcctgcct
     8401 ttgccgttgc cgagaccgta ggcgatgttg cggtaaacgg tcaggtgggg gaacagcacg
     8461 ccttcctgta cgaggtaacc caaacggcgt tcgcggacgg gaaggttggt atttttcgag
     8521 aagatggttt tgccggaaag cgaaatttcg ccggaatcgg gttgttcgaa accggcaagg
     8581 cagcgtaaaa gggtggtttt gccgcagccg gacgcgccga tgataaagag aatttcgccc
     8641 gggtcgaggc tgagcgaaat gtcgtttaaa accggggtgt tttgaaaact tttggacagg
     8701 tgtccgatgt gcagggcggc ggtcatagcg gtgcttcctc aagctgttat ttgaaggcgt
     8761 atttcttcag caggaatacg ggtatgccgg aaaataatac cagcatcagc gcgtaagggg
     8821 cggcggcggc gtattgtgcg tccgatgtgt attcccaaac ggcggtggag agcgtgtgga
     8881 catcgtcggc ggtcagcagc agggtggcgg tcaactcttt catcagcttg aggaagacga
     8941 gtgcgaatgc ggcggtaatg ccgggcagga tggacggcag taccaacgtc ctgaaaataa
     9001 agaagtgtcc gcgccccaat gttgcgccga cctgttccat ccctttgggg agttgttcca
     9061 aggaagtcct cagggtggtt tgcgccatcg gcaggtaaag catgaaatag gcaaggatga
     9121 cgacgataaa ggtttggtaa acggcagggg tgtagttgat gctgaaataa accaaggata
     9181 gggcgataac caaaccgggg acggcgtgca gtaaaaacgg cagcctgtct atccaaacgg
     9241 ttaaaaaatt gcgatagcga accgatgccc aaacaagggg caaggcacat aatatagtca
     9301 aaatcgcacc taaagccgat acgcttaagg aacggataaa ggcatcaaat acggatacga
     9361 gcgcgaatgt gccggaagtg ccgaccatca tccaatgtat caatacgcca aaggggataa
     9421 taatgcccaa aatcaacaag ctgcttaaaa aaacaatcgc gccgatctga ccgggcagtt
     9481 tgagggtttt gacgggataa ggacgggcaa cgcctttgcc gctgtggtaa atcttggctt
     9541 tgccgcgaaa tatgctttct ccaaatacga cgatgccgca caccgccgtc aaaacagcgg
     9601 aaagcagggc ggcggtattg ttgttgtagg acatttcgta ttcttggaaa atggcagtgg
     9661 taaaagtggg gtagttcaaa atggataccg cgccaaattc gaccagcata tgcagggcga
     9721 tcagtaacac gctgctgccg atggcgggtt tgagctgtgg gaggatggcg gaaaaaaagg
     9781 tttgcaggcg gcttttgccc aaggacaggc tgacttcttc gtaagacagg ctgatgcgtt
     9841 tgagtgccgc ctcgacgggc aggtaggcga gcggaaacga ggacaggctc ataatcatca
     9901 ctgtccccca aaagccttcg acgcggaagg tcaggctgat ccaggtgaaa cagctgacaa
     9961 atgcggggat gcacaaaggc agggtgattg ccgtctgaaa aaaggttttg ccgaagaagc
    10021 ggtaacgttg gaacaaaagg gcgcaggcaa tgcccaaaac aatggaaatc agggtaacgc
    10081 ccgccatcat cgtcaaggtg ttggagagca aatcccacat acgcgggcgg aacaacagtt
    10141 cgacggcgcg gttgatgccg acctgccacg aacgcatagc gacatataaa aaaggcaggg
    10201 taagcggcag ggcgatcagt aggatgaggc cggtaagcca aatgggtatt tttttaggag
    10261 acatagtgtt tttcatcggc aaaacgggcg gacagtataa atgtccgccc gtttgacaat
    10321 ccgaaaacgg cttatttcat accggcttgc tcaagcagcc gggtggcgtg ttctttttcg
    10381 gaaacagtgg tggcggacac ttggggtgct tccaacttgg cgatgggttc caaattgaag
    10441 gtggatacca cgtgcggatt caaaggatat tcggcacgga cggcggtcag ggcgcgctgt
    10501 ccttccttgc cggcgaggaa ggcgacgaat tttttcgcct catccttgtt ttgggaggat
    10561 tttaacacgg ctgcgccgga ataggtaacg agtgcgccgg gatctctgtg gcggacgaaa
    10621 ttcaggcggg tgtggacatt ttgtacgcct ttttcacgcg cgaaagcgtg ccagtagtag
    10681 ttgttgatga gggcggcatc gatttcgccg ttttcaaccg cttgaagggc gacggagttt
    10741 ttagcgtaag gcttgccgta ttctttcagg cctttgagcc atttcaatgc ggccgcttcg
    10801 cctttcagtt tgacgatggc gacaatctgt tccaagaacg cgccggaagt ggggacgtaa
    10861 ccgatgcggt ttttccattt cggcgtggcg taattcagga cggatttttc caaatctttt
    10921 tcagacagtt tgcgggtgtc gtaaacgacg acgcgcgaac gtccgctcag tgccacccag
    10981 tcttttttgg cggcaaccgg cacgcctttg ccgcgtgttt cgttgatggt ggaggcgggc
    11041 aggggctcta ggaggttggc tgcggaaagg gtggcgagtg ccgggatttg ttcggaatag
    11101 aatacgtcgg cggggcttcg gctgccttct tctttgattt ggccggcaag ctggtcgcct
    11161 ttggcactgt tgagtttgac tttgatgccg gtagcccggg taaaggcatc tgcaacggct
    11221 tgtgccgctt ctttgtgttg gccgttgtac acggtaatgt ctgccagcgc gggggtggcg
    11281 gcggtcaggg ctgcggcaag cagtgcgtat cggatagatg ttttcatatc gattttctcc
    11341 taaatgaatg agggtgtata ccttgttaag acataacggg gtgtagtgta ttccttcttt
    11401 tttataaatg caaataatta ttttttaaat ttgttattat ccgatccggt tattgtttgt
    11461 tctgacttgt attttttccg tgcatcgcgc ccgtaaggcg gaagcggcgg gcaatgcgtg
    11521 gcggaatgtg ggtaaaggcg gcattttgat ttgtcggaat gctggagaac ctctcccttt
    11581 aaaacgccgt ctgaacaagg ttgccggaat agtattgcca tcccggcaga tacagtttgt
    11641 cgggatcttg ccaatattgt tcatccagac tgttcggcag cgaggcggtt ttgttgtcga
    11701 gatgttttgt taatccacta tatatcccac atttctttta ggtttttacc ttccgattgg
    11761 aggtggcgga tttcttttcc caatgcgtct gccgcccgat ggatcagcat tgcgctgtgt
    11821 accgcgccga tttttttcag aacggaacaa gtcttttcgg cggcgggaaa accccagttg
    11881 cagacaaatt gcagtatgcc gccgttgccg atgtcggctt ccgcccgcca aatcaaaacc
    11941 agctcctgtt cccgtccgtc catgctttcg agtttgccgt catgctgttc aaaaagtttg
    12001 tccaccgcct gccgcatcat ctgctcgaag cggtcggctt gggtatccgt atcggtcata
    12061 atattcttgc cttttaaaaa tgccgtctga acatttcttc agacggcatt tgggggttaa
    12121 gctgacattt cccgccagcg tttgacttgg aggcgtacct gttcgggcgc ggtgccgccc
    12181 aagtggttgc gcgcgttgag gctgccttcg ggtgtcagaa cgccgtacac gtcgtctgaa
    12241 atcagcttgc tgaagccttg cagggcttcg agcggcaatt cgctcaaatc aacgccggct
    12301 tcatcggcgt ggcgcacggc ttgggcaacg acttcgtggg catcgcggaa gggcatacct
    12361 tttttgacca gataatccgc caagtcggtg gcggtggcaa aaccctgcat cacggcggcg
    12421 cgcatattgc cgggtttgac ggttacgccg cgcatcatat cggcgtaaat ccgcagcgtg
    12481 tcgatgagcg tgtcggcggt gtcaaacagc ggttctttgt cttcctgatt gtctttgttg
    12541 tatgccaagg gctgcgattt catcagggta atcaggccga taaggtgtcc gatgactcgg
    12601 ccggatttac cgcgcacgag ttcgggtacg tcggggtttt tcttttgcgg catgatggac
    12661 gaacccgtac agaagcggtc ggcgatgtcg ataaagccga aacgcggact catccacaaa
    12721 atcaattctt cagacaggcg gctcaagtga accataacca gcgaggcggc ggcggtgaac
    12781 tcaatggcaa aatcgcggtc ggatacggca tcgagcgagt tctggcagat ttgttcgaaa
    12841 cccaacaatt cggcggtgat ttcgcgctga atcggatagg tcgtaccggc aagggcggca
    12901 gcgccgagcg gcatacggtt gacgcgtttg cgacagtcgg ccatgcgttc gaaatcgcgt
    12961 ccgagcattt cgacgtaggc gagcatgtgg tgtccgaagc tgaccggctg ggcgacttgc
    13021 agatgggtaa aaccgggcat gacggtttcg gcgttttgtt ccgccaaatc caataatgcc
    13081 gtctgaaggt tttgaatcag gttttggata acggtaatct ggtcgcgcag ccaaaggcgg
    13141 atgtcggtgg cgacttggtc gttgcggctg cggccggtgt gcaggcgttt gcccgcgtcg
    13201 ccgattttgt cggtcaggcg gcgttcgatg ttcatgtgga catcttccaa atcgagcggc
    13261 cattcgattt tgccgctgcg gatttcttcg aggatttccg acatgccccg acggatgtcg
    13321 gtcagatcgt tttcactcag cacacccgac cgggtcagca tctgcgcgtg ggcgagcgag
    13381 ccttggatgt cccattcggc aaggcgttgg tcgaaaccga tggaggcggt gtattgtttg
    13441 acgagttcgg aaacgggttc gttgaaacgt ccggaccagg ttttgtcgtg cataaggatt
    13501 ccttgatggg gttattcggt gcggtatttt tccaaaaggc ggcggaaggg ttcgccggtt
    13561 tcggggtgtt ttaaaccgta ggcgacggtg gcttcgagat agccgagttt gctgccgcag
    13621 tcgtagcgcg tgccttcaaa ggggtgcgcc aagacaaatt cgtgatcgag cagcttggcg
    13681 atgccgtctg taagctggat ttcgttgccc gcgccgcgcg gcaggccggt taaaaggtcg
    13741 aaaatgcgcg gggtgaggat gtagcgtccg acaacggcaa ggttggaggg cgcgtcttcg
    13801 ggcttgggtt tttcgacaat gccggtaatg cgttggaact gtttgagctg ttcggtttcg
    13861 acgatgccgt atgagccggt ttgcgacggt tcgacggttt ctacgcccaa aatgctgtta
    13921 ccgctgcgcc cgtacacttc gaccatttgt ttgagcgcgc ctttgggcgc atcgattaag
    13981 tcgtcggcaa ggatgacggc gaagggttcg tcgccgatgg cggcgcgggc gcacaagacg
    14041 gcgtgtccca atcccagtgc ttccgcctga cggatgtaga ggcaggtaat gttcggcggc
    14101 aggatgttgc ggacgtgttc caacaatttg tctttatggc gcatttccaa cttagtttcg
    14161 agttcgtatg ccttgtcgaa atggtcttcg atgctgcgct tgttgcgccc ggtaacaaac
    14221 accatttccg tgcagccggc ttccacggct tcttctacgg cgtattggat cagcggcttg
    14281 tcgacgatgg gcagcatttc tttcgggttg gccttggtgg cgggcaggaa gcgggttccc
    14341 atccctgcaa cggggaagac ggcttttttt atcggtttca ttctttttcc tttgtattgt
    14401 tttgatgttt aaagggcgag tttgcggatt aaatcggcga gtgcctgcgc gcggtggctt
    14461 tcgcggtttt tgacctccga atccaattcg gcggcggttt tgccgtgttc gggcagataa
    14521 aaatacgggt cgtaaccgaa accgttttgc ccgagcggcg tgtcgtgcca ttgtccgtgc
    14581 catatgccct cggcgataat cgggcgcggg tcgtctttat gacggacaaa aaccaatacg
    14641 cagacataag aacaactttt gtcggccttg ccggcaagtt cggcggcaag tttcaggttg
    14701 ttggcggtat cggatttggg attgctgccg gcgtaacgtg cggaatggat gcccggcgcg
    14761 ccgtttaggg cggcggcaca gatgccgctg tcgtcggcga gtgcgggcag cccgctgtgt
    14821 ccggcggcgt gccgggcttt tgccagcgcg ttttcgacaa aggtgggata gggttcgggg
    14881 cattcgggta tgccgaatgg ggattgcggc aatacggtga tgccgtaagg tttgaataag
    14941 ttgccgaatt ctttgagctt gccggcattg ccgcttgcca aaacgatttt ttccggtttt
    15001 tcagacatag cggtttccct gtgatgaaga tggggcggcg cgtagggatt tgtgccgcag
    15061 gtagagggcg agtgcgccga tttgcccgaa cagcgcgccg aatgcgaaaa ggaaggacgc
    15121 gacggcgaag gctttgctgc cggcggtcag cagataagca cccaagagga cgggcagcat
    15181 aaatgccagt gtgaacaagg caacagacaa cagatagatt tttcggcggt tcatggcgtt
    15241 cggtcggaaa cgggatgttc ggattatagc cgattaggac ggcattccct agaggctgga
    15301 aaaatgccgt ctgaagcggc gttcagacgg catggcgggc attatgcctg tttgttccaa
    15361 cgttcgatgg attctttgat gacttttttc gcttcttccg caccgcccca ggattcgact
    15421 ttggtcgtac ccgctttttt caggtctttg tagtggttga agtggaactc gatttgtttg
    15481 atgagctgtt gcggcaaatc ggacaaagtt ttgtaggcgt tgccgttatt gcggtcgtcg
    15541 gcaggtacgc agacgatttt gtcgtccact tcgccgtcgt cgacgaattt catcacgccg
    15601 ataacgcgcg cttccaagaa tacgccggtt gccagaggtt gttcggtaac gagcaacacg
    15661 tccaattcat cgccgtcttc gtccaaagtt tgggggatga agccgtagtt ggtcggtttg
    15721 gcgaagatgg cgggttcgat gcggtcgagt tggaatgcgg cgagtttgcg gttccactcg
    15781 attttgtggt tgctgccggc ggggatttcg ttgacgacgt tgatgatgcc gccgtccacg
    15841 tcgcccgggg tcaggatttg gttgaagtct gccatttggt ttcctttatt tgggaaggtt
    15901 tgaagtttga aagtatagca caaacgtccg gctggaaatg cgtccgatgc cgtctgaaag
    15961 ggaaaccggg cgcgtgttac cgtttgccca aaaacctgcc cagttccaaa atcgcgcgcc
    16021 tgttggacgg ggaaaacact ttttccgccg cttcttccaa gccgaaccag ccgtaggaga
    16081 cgtgttcctc gggttgcagg acgacgggcg tatcgcgcgg gatttcggcc cggaagacgt
    16141 gttcgcggtt ttcaaacacg cctttggggt agcggtgccg ccagtggtgg tagatttcgt
    16201 aaaccgtgct gtcgtgccgg tcttggagct gcccgtcctc cagcaggatg ccggtttctt
    16261 cccaaacttc gcgccttgcc gtttgggcga cggtttcgcc cggttcgagg ctgccggtta
    16321 ccgactgcca aaatcctttc ggatgcgtgc gttcgatgag caggatgccg ccgtccccgc
    16381 tataaaggac gaccagtgcg gaaacggggt atttgagcgg ttttgccatc ggcatctttc
    16441 ggcgggctgc ggtaatgaag gggcggatta tagcaaacgc cgcacgttat ggcgtttatc
    16501 cttttccgta tccttttttc tgcacggatg ggacgcgccg gtgtttgccg gtaaattttc
    16561 ctttgtgtca aaaagataag ggcggttgtg attttaatgc ttgccaaagt gacgggcgga
    16621 aactataatc cgcgacttac cgagtcggag tgtggcgcag tctggtagcg cacttgcatg
    16681 gggtgcaagg ggtcgaaggt tcgaatcctt tcactccgac caaaaattcc gaaagccgct
    16741 ttcaaaagcg gcttttttgc cgtccgtatg attatgatgt agagtacgcg gcgacagaca
    16801 ttcaaatgcc gtccgaaaac cgttcagacg gcatctcttt atcttaatag tggattaaca
    16861 aaaatcagga caaggcgacg aagccgcaga cagtacaaat agtacggcaa ggcgaggcaa
    16921 cgccgtactg gtttaaattt aatccactat agtttcattc cgtaccatct taaggaacat
    16981 caaattgggc atttcccgca aaatatccct tattctgtcc atactggcag tgtgcctgcc
    17041 gatgcatgca cacgcctcag atttggcaaa cgatcccttt atccggcagg ttctcgaccg
    17101 tcagcatttc gaacccgacg ggaaatacca cctattcggc agcagggggg agcttgccga
    17161 gcgcagcggc catatcggat tgggaaacat acaaagccat cagttgggca acctgatgat
    17221 tcaacaggcg gccattaaag gaaatatcgg ctacattgtc cgcttttccg atcacgggca
    17281 cgaagtccat tcccccttcg acaaccatgc ctcacattcc gattctgacg aagccggtag
    17341 tcccgttgac ggattcagcc tttaccgcat ccattgggac ggatacgaac accatcccgc
    17401 cgacggctat gacgggccac agggcggcgg ctatcccgct cccaaaggcg cgagggatat
    17461 atacagctac gacataaaag gcgttgccca aaatatccgc ctcaacctga ccgacaaccg
    17521 cagcaccgga caacggcttg ccgaccgttt ccacaatgcc ggcgctatgc tgacgcaagg
    17581 agtaggcgac ggattcaaac gcgccacccg atacagcccc gagctggaca gatcgggcaa
    17641 tgccgccgaa gctttcaacg gcactgcaga tatcgtcaaa aacatcatcg gcgcggcagg
    17701 agaaattgtc ggcgcaggcg atgccgtgca gggtataagc gaaggctcaa acattgctgt
    17761 catgcacggc ttgggtctgc tttccaccga aaacaagatg gcgcgcatca acgatttggc
    17821 agatatggcg caactcaaag actatgccgc agcagccatc cgcgattggg cagtccaaaa
    17881 tcccaatgcc gcacaaggca tagaagccgt cagcaatatc tttacggcag tcatccccgt
    17941 caaagggatt ggagctgttc ggggaaaata cggcttgggc ggcatcacgg cacatcctgt
    18001 caagcggtcg cagatgggcg cgatcgcatt gccgaaaggg aaatccgccg tcagcgacaa
    18061 ttttgccgat gcggcatacg ccaaataccc gtccccttac cattcccgaa atatccgttc
    18121 aaacttggag cagcgttacg gcaaagaaaa catcacctcc tcaaccgtgc cgccgtcaaa
    18181 cggaaagaat gtcaaactgg cagaccaacg ccacccgaag acaggcgtac cgtttgacgg
    18241 taaagggttt ccgaattttg agaagcacgt gaaatatgat acgaagctcg atattcaaga
    18301 attatcgggg gcggtatacc taaggctaag cctgtgtttg atgcgaaacc gagatgggag
    18361 gttgatagga agcttaataa attgacaact cgtgagcagg tggagaaaaa tgttcaggaa
    18421 acgagaagaa ggagtcagag tagtcagttt aaagcccatg cgcaacgaga atgggaaaat
    18481 aaaacagggt tagattttaa tcattttata ggtggtgata tcaataagaa aggcacagta
    18541 acaggagggc atagtctaac ccgtggtgat gtacgggtga tacaacaaac ctcggcacct
    18601 gataaacatg gggtttatca agcgacagtg gaaattaaaa agcctgatgg aagttgggag
    18661 gtgaaaacga aaaaaggtgg gaaagtgatg accaagcaca ccatgttccc aaaagattgg
    18721 gatgaggcta gaattagggc tgaagttact tcggcttggg aaagtagaat aatgcttaag
    18781 gataataaat ggcagggtac aagtaaatcg ggtattaaaa tagaaggatt taccgaacct
    18841 aatagaacag catatcccat ttatgaatag taatatttat gaaaaattag gagattaatg
    18901 atgaaaagaa ttaagtgctt ttgtgataaa tttccatcag gagatacatt tagaatgtgt
    18961 atcattctgg atgactatga taatagggtt gattattatg taggaatata tgattacatt
    19021 acgtctacct taatgagcga tatttactat cgatccacga ttgatgagca tttcaagatt
    19081 atagaattaa tagaaaataa tccaaatgaa atttatgatg atggcggtgg tcaacaattt
    19141 tgcctagaat ttcatcatga taaggtcatt ttttatcaca atgaatttga tgaagaagat
    19201 ggttatccag tattaagctg ttcgctgcat acttttaaaa ctgctttaat tgcttggaat
    19261 gcttttttgc aattgcctaa aagtattcat tcggtggtgg agactgtgat tgaggaataa
    19321 gcataattag cttaatgaat agaatcagcg atatagattg gactgcaaat ccacgcttat
    19381 acgctgtgcc atgattaaca ggaagagagc aacttggatg ggctagtagg gatggtaagc
    19441 atttaaatat atcaattgat ggaaagatta cacacaaatg actaatttaa aattagattt
    19501 ctactctgaa gttattataa aagattcttg ccctaatgat ttgttagaaa atggtgagac
    19561 tattaaagga aaaaaaggag tggtattagg tataagcgaa gaagatggta taatctatgg
    19621 ctatactatt ttactttttg atataaaata ttgtatatat atagataaga aatatattat
    19681 accgactgga aaaaaattct ctcgcgatga tttttattag ctaatttaag taatttaagc
    19741 aagtttttta gatactgaaa ctggtaatta tgtttatgta agcataagga gttcagattg
    19801 aaggattcta cagggcaaat gaggctggca accaaggatt tggcggaagc cattaaacga
    19861 ggagaagtac gtagttctgc ttttacaaca aagcaactaa aggcaatcga aaaaggcaaa
    19921 gacaaaatcc ctagctacac ttggcatcat catcaagata cagggagaat gcagcttgtg
    19981 cctgaatggg aacattctaa aaccggtcac ataggaggga cggcaatggg gaagggtaaa
    20041 taattatgtg gaaaatttat aaagaaaata gcaccgactt aaattttgcc ataggcagta
    20101 tatattgcca agcaatcaat cttaccgaat ttaaaatgtg ggtcgaaaaa atcataaggg
    20161 aagtggattt agatgaaatt ccaaattatt tttttgattt gatagatttt caatcactat
    20221 acgatctgat tgatattata ggatttgttc ccgaaaataa cttatcaaaa aatcaggata
    20281 atgcattaac cggtattgct tttttaaggg ggatagatgt ctacgatcct cccatttcaa
    20341 aagaaaaagc attaaaagcc ttagaaaaac atcctgaaat ttatcagaag tttcagcatt
    20401 tctttccgtt tgtagagctg cctccgcttt aaacagtcaa aatgccgtct gaaacgatat
    20461 tcggctttca gacggcattt ttgatataaa gcggataacc aaagaaatgt ttgacggcaa
    20521 aggaacatct gaaataccaa attacacttg gcatcatcat caagatacag gaaggatgca
    20581 attgattcgt gaagactcgc atcatgatac cgaccatatc ggttggagag cgatgagtaa
    20641 aggaaagtaa ctatgtggaa aatcataaaa gaggatagtg atgatttagg atttgcaatt
    20701 aaatgcttat tctctcaatc tattgattta aatgaattca agttatggat tgaacaagta
    20761 atacgcgata tgcccatcga ggacatccct ttttatattt ttgacttggc ggatttcaat
    20821 gggggaattg gagacattga caatattgta ggttttgttt cgagttacag cctatcaaaa
    20881 tcgaaaaaaa atgccttgac aggcattgcc ttcttaaggg ggatagatgt ctatagaccc
    20941 tcccgtttca aaagaaaaag cattaaaagc cttagagaaa tatcctgaaa tttatcataa
    21001 atttaaacgg ttctttcctt ttgtagagct tccgcttctt taaaggacaa tatgccgtct
    21061 gaaaagtttt cagacggcat ttttatttct tccagtaggc ggaggtgaag aggatgaaga
    21121 cggtgaagat ttccagcctg cccaagagca ttgcggtaac gcagatccat ttctgcatca
    21181 cgtccaaacc ggcgtaattg ccggcgggcc cgacttcgcc cagtccgggg ccggcgttgg
    21241 taatgcaggc gatgacggcg gtaaaggcgg tggtaaattc cataccgctc gccatcagca
    21301 ggaagctgaa gaggacgacg gtcataaagt agatgaagat gaaggacata acggtcagcg
    21361 cgaggcggtc gggtatggct ttgccgctga ttttgacggt gcggacggct ttggggtgca
    21421 gcagcaccat catttcgcgc aggctgaatt tgaacaggac gagggcgcgt atggttttga
    21481 tgccgccgcc ggtcgagccg gagctggcga ggatgttggc gaggaaaaac atccacaggg
    21541 aaatcaggag cggccattgt gcgaagtcgg tgttggacag cccgtttgcc aatccgatgg
    21601 agacgaagtt gaaggcggtg tagcgcaggg attcgccgaa gccggcgtaa tggccggtgt
    21661 gccacaggta cagggcggcg gcaaggatgc tgccggagag cagcagcagc atcgtccggc
    21721 attcttcgtc tttccaatag gttttgaggc tgcggctgtt gagggcggcg aaatggctgg
    21781 caaaattgat gccgccgaaa atagtgaaaa cgatgatgac tgcttcgatg aggggggagt
    21841 tgtaataacc gatgctggca tcgtgggtgg aaaacccgcc cagcgagagg gttgccagcg
    21901 cgtgacagac ggcatcgaac caacccatcc cggcaaaatg caggcaggct gccgcgagga
    21961 tggtgatcag ggtgtagccg aaccagagtt ttttcgccac ttgggaaatg cgcggcgaca
    22021 ttttgctttc tttgtcaatg ccggggattt cggctttgaa taactgcgtg ccgcctacgc
    22081 cgagcatagg caggatggcg acggcaagga cgatgatgcc catcccgccc agccagttga
    22141 gcatatgccg ccaaaagttg acggaggggg cgagcccgtc gacgtggggg atgacggtcg
    22201 cgccggtggt ggtcagtccc gacatcgatt caaaaaatgc gtcggtaaag cccatattcg
    22261 ggaaatacag gtacatcggc atcgcagcca tagcggcaaa cgccagccac aacatcagga
    22321 cgagggtgaa gccgtcgcgc gggcgcagtt cgcgcctgaa ccggagggtg gcgagccgga
    22381 cgatgcacga gccggaaagg gtaacggtcg cggtggtggc gaatgcggtg tacgcgccgt
    22441 ccgaaaaggc gtaggaaagg gcggcgggta tcagcaggat aaaggaaaac agcataccca
    22501 gtcgggagag gacgtgggcg atgggcagga ttttgtgcat agtggggcgg tccgttattt
    22561 tgcgaagctt ttccagtcta tgccgccggc ggcttggact tgggtaattt cttccgtttc
    22621 gaggttgacg gcggtcagct gcccgcccca cagcgcgccg gtgtccagcg agatgacgtt
    22681 gtcggcattc gtgtagccca gcgaggacca gtgtccgaag atgatggtgt ggtcgaggtt
    22741 ttgccgatcg ggggctttga accacgggcg caggtaaggc ggcatttttt tcactgtgga
    22801 tttgtagtcg aaatccagtt cgtttttaaa ggtcagggcg cgcatccgcg tgaaggcgtt
    22861 gacgatgaag cgcaggcggg catagccttc caaaccttcg tcccatgcgg ccggtttgtt
    22921 gccgtacatt ttggagaaga atttgacgta ttttttgccg cgcagttcgg cttcggcttc
    22981 tccggcgagc gattcggctt tggctatgcg ccattgcggc aggatgccgg cgtgtatcat
    23041 cacgcggccg ccctcgcgta tcaaaagcgg ttgcgcgcgc agccagtcga gcatttttcc
    23101 gccgtcgggg tgtttgagta tgggttcgat tgtgtcgctg cgtttgagcg cgccttcgcc
    23161 gcagccgacg gcgagcaggt acaggtcgtg gttgccgagg acgatttgca cgctgttttc
    23221 gtgccggatg cagaattgca gcgtttcgag ggatttcggg ccgcggttga cgatgtcgcc
    23281 cgtcagccag agggtgtccg tgccgtggtt gaagccgatt ttgccgagca gcgcggtcag
    23341 ttcgtcgaaa cagccttgga tgtcgccgat tgcgtaatgt gccattgcgg gtgttgtgaa
    23401 gtgggaaagt gttgcggttc ggacggcacg gttggaaatc ttatgccgtc cgaacgtgga
    23461 attatgcgtt caaaacgagg acggcttccg cttcgacctg cacgcctttg ggcagcgagg
    23521 caacgccgac ggcggcgcgg gcggggaacg gctcggtgat aaattccgcc atcacttcgt
    23581 tgaagacggc aaaattgccc aagtcggtca ggtaggcgtt gagtttgacg atgtcgtcca
    23641 gcgaaccgcc tgccgcttcg gcgacggcct gcaggttttg gaacacttgg cgcgcttcgg
    23701 tgtggaaatc gccgttgccg accacagtca tcgtggcggg atcgagcgga atctgaccgc
    23761 tcatgtaaac ggtgccgccg gcgcgcacgg cctggctgta tgcgccgatg gcggcggggg
    23821 ctttgtcggt atggatgacg gttttggaca tttcggattc ctcaaaaaat agggcggcag
    23881 aagccgcagc gttcgggatt atcgtacaaa accgccggct tgtgtagttt taggcggtat
    23941 atgtgccgga aacaaaaaac cgccgaaggc tcggcggttt gcggaataac gcgtatatca
    24001 gaatttgacg cgcacgccgg cggacagttc gccggaacgg acgtttttga cattgttgac
    24061 tttgccgatg tagttgtagc ggtagccggc atccagatcg acattgggtg taacggcata
    24121 gcttacgccc gccaatacgc cgaggccggc ggaggttttg ctgaagctgt cgctgccgcc
    24181 caagtgggcg gaagcgcggt tgaggctcaa gcgcgcgccg aaatacggtt tgacgggcga
    24241 ttgggtgtcg aagtcgtaaa tgacggacgc gccgatgctg taaagtttga aatcggtgga
    24301 tggggcttgt ttatagtttt tgtagcgcgt gtaatcgacg gcgaagcgga ggtcgttgat
    24361 gcggtagcct gcggagatgc gcgggctgaa gcctttggca gaacctaaag agcttgaggc
    24421 tttggcgtgt gcggcatcgg cttggacgta aaagccggat gcgccttccg ccagtgcggc
    24481 ggccgggagt gcgagggcaa tcagtgcggc aagtgctttt ttcatatttt ggttccttta
    24541 tggtcaggga gaaaaattgt taataatccg ttaaagaatc ctgccgtatt atactgaaat
    24601 tttctttttg catcgtaata ttttcaatgc ctcaagatac gcggcggcat cacgtggcgg
    24661 atgggtgaaa cttgcaaacg gtttggaaaa aaccgcaaag gcggggcggc ggcatttgtc
    24721 agattgttgc aggcgcaggc gtacggtttt ttgtgtgcgg ggttacctta ggcgtcggac
    24781 atttccggcg gcggctgtgc cgtctgaaac gcccggcggg ggatgcggct gcgtttccca
    24841 tcgataggca tatttgccgg ccgcgttcgg ggcgggtttt acccgggcgg ccgccgattt
    24901 gtttgcgctt ataaaaaaca ctgcaacaaa tcgtttaaaa acaagcgtcc tttttcggtc
    24961 gggcggaata cggtcgggtc ggtttccagc aggccttttt gccttgccgt ttcgatttgc
    25021 accatgattt tggcggcggg tacgcccgtg cgctcctgca acatcgcggc gggtacgccg
    25081 tcggtcaggc gcagggcgtt catcatgaat tcgaacggca aatcttcggc ggcgacggtt
    25141 ttgcgttcga cggcttcgcc cggctgactt tgcattaagg cgaggtagtc gttggggtgg
    25201 cggcggcgga cggtgcgctc gatgcggtcg ggataggaga ttttgccgtg cgcgcccgcg
    25261 cctatgccca aataatcgcc gaactgccag tagttcaaat tgtggcggca ctgcatggtg
    25321 ggtttcgcaa aagccgatgt ttcgtagtgg ataaaacccg cgccttccag tgtgccgtgt
    25381 accgcgtctt cgatgtcgag cgccgcttcg tcttgcggca aacctttcgg cggcgtatga
    25441 ccgaacggcg tgttcggctc catcgtcaga tgatacgcgc tgatgtgggt tgcgtctgtg
    25501 gcgatggcgg tttgcacgtc gtccaatgcc gtctgaacgg tttggttcgg cagggagtac
    25561 atcaagtcga tattgacttt atcaaataat tttaaggcgg tatcgatagc ggttaaggct
    25621 tctttgccat tgtggacgcg ccccagcctt gagagcatat cgtcgttgaa actctgtacg
    25681 ccgatagaaa gccgcgtgat gcctgcgtct ttaaatcctt gaaacttctc gatttcaaat
    25741 gtacccggat tggcttccaa cgtaatttcc gcttcgggct gcaagcgcaa cagcgaacgc
    25801 acgccgctta acaaacggtc aatcgattcc gcctgaaaca ggctgggcgt accgccgccg
    25861 aaaaatatcg tttccaccgg cctgccccaa atattgggca attcaagctg caagtcggtc
    25921 agcagcgcgt cgatataggc ggcttcgggc aatccgtttt tcaggctgtg ggaattgaag
    25981 tcgcaatacg ggcatttttt gatgcaccac gggatgtgga tatagagcga caggggcggc
    26041 agggcggtga gtcgggtgcg gtttggaaag gaaatggtgt gcatggtgcg gttcggaaaa
    26101 gtggacgatg ccgtctgaag gcggttcgga cggcatgggt tcagcagagc agggtaagca
    26161 gttcggcttc gctgaggacg gaaacgccca aggcattggc tttttccagc ttgctgcccg
    26221 cggcttctcc ggcgacgacg tagtcggttt ttttggacac gctgccggac actttgccgc
    26281 ctgcggcttc gattagggct tgggcttggt cgcgtttgaa ggtgggcagg gtgccggtta
    26341 acacaaaggt tttgcctgcc acggctttat tgatgccgtc tgaaccttgc gccgcctcgt
    26401 cttcagacgg catttgcgcg aagaaggttt ttaggttttc gagcagggcg gtgttttgcg
    26461 ctttgctgcg ccacgcctgc cagtcggcag ggagggcttt gtcgttttgc agtccttcta
    26521 tactttgtcc ggcgagttcc cataaggctt gggctttgtt ttcgctgatt ttgaaaccgg
    26581 gcaggcgggt aatccagcgt tgcggcccgg catattgtgc ggcaggcagg ctgacggctt
    26641 gggcttgcgg ggcaacgcct gcggcgagca gctcgtctat catcgcctgc tgttcggctt
    26701 gggcgaagaa gtgggcaatg gaacgcgcca ctaccgtgcc gatgtcgggc aggcaggcga
    26761 ggacgggttc gggggcgcgg cggacgcgtt ccaatgtgcc gaatgcctgc gccagcgttt
    26821 tggcggtgcg ttcgccgacg tggcggatgc cgagcgcgaa caggaagcgg gcaagttcgg
    26881 gcgttttgcc ggattctatg cctgcgagga tgttttgcgc ccacttggtc ggttgttttt
    26941 tgccgttttg ggtattgtat ttagacaggt cgcctgaaac cgtttctgcg tcgccgtttt
    27001 cgttttcaga tgacccttta tccgccgttt ccttcatttt ttgcagggtc ggaatatcga
    27061 tgcggtagag gtcggcgaaa tggcggacga ggtcttgggc gaccagctgt tcgatttgtt
    27121 tttcacccaa gccgtcgatg tccatcgctt tgcgcgaggc gaagtggatt aagccttgcg
    27181 cgcgttgtgc ctgacaaagc ataccgccgc tgcatcgggc gacggcttcg ccttcttcgc
    27241 gttcgatttc gctgcggcag atggggcagc gggccggcag gcggtagggc ttatggagcg
    27301 gaacggattc ggtttgcttg gcggacggtg tttcggcaaa caggtcgtcc tgctgatgcc
    27361 cgatgccgtc tgaaacggca acggcggttt cctgcatcgg gcggcgttcg aaaatcacgc
    27421 gcacgacttc gggaatcacg tctccggcgc ggcgcacgac gacggtatcg ccgacgcgca
    27481 cgtctttgcg cgatacttcg tcctgattgt gcagggtggc gttggtaacg gttacgccgc
    27541 cgacgaatac gggttgcagg cgggcaaccg gcgttaccgc gcccgtcctg ccgatttgca
    27601 cgtcaatcgc ttcgacaatg gtcagggctt cttcggcagg gaatttgtgg gcaaccgccc
    27661 aacgcggcgc gcgggagatg aagccgagtt cgcgctgttg cgccaagctg ttgactttga
    27721 ccaccatgcc gtcgatttcg tagggcagtt cggggcgttt ttgctgcatg tgttcgtaaa
    27781 acgccaatac ttcgccgata tttttgaaac agccgaaatt gccattgggc agactgaagc
    27841 cgagttcttg gaaataggcg agttcttgga tgtgttcttc agcttcgaaa ccgccttgct
    27901 gacgggcaat cgaatagggg aaaaagtgca gtttgcgttg cgcggtgatg cgcgaatcga
    27961 gttggcgcag gctgccggcg gcggcgttgc gcggattggc aaagggtttt tgcccgtttt
    28021 cggcttggcg tttattgagc ccggcaaagt cggctttgag catcagcact tcgccgcgta
    28081 cctcgatgag ttcgggcacg ttttcgccgt gcagccgcag ggggatgttg gatacggttt
    28141 tgacgtttcg ggtaacgtct tcgcccgtcg tgccgtcgcc gcgcgttgcc gcctgcacca
    28201 atacgccgtc gcggtagagc aggctgatgg cgaggccgtc gaatttgggt tcgataacgt
    28261 attcgggatt gccgccgtcc aagccgtcgc gcacgcgttg gtcgaaggcg tacatttcgg
    28321 catggtcgaa cacgccgttt tcatcttgcg gggaaaaggc gttggtcagc gacagcatcg
    28381 gcacttcgtg gcgcacttcg gcaaagcctg ccaaaggctc gccgccgacg cgctgggtcg
    28441 ggctgtcggg cagcttgagc tcgggatggt ttcgttccaa cgcttcgagt tcgcggaaca
    28501 ggcggtcgta ttcggcatcg ggtatgctgg gcgcgtcgag ggtgtagtat tcgtaggcgt
    28561 agcggttgag gaggtcggtg agttcgtgta tgcgttgtgc ggtcgggttc atggattcgt
    28621 acaggttcag acggcatgga caaatgccgt ctgaacggta aaacgtgttg gaaatggtcg
    28681 gattttacct gaaaacaaaa ggcggatgca ccgttgccga tgtatccgcc tttgtgtttg
    28741 cggagttatg aaaacaggcg cagggcggtt ttgccgcccg gttcgatacc gactttgagc
    28801 atctcggact gacgcgccaa tacataagtg cgtacgtctt tgagccattg ggtcgaaact
    28861 tcttccattt tgtcgttgac cagattcagg ttcaactgac cggacaggcg taccgccaaa
    28921 tccataaaca aatcgtcgaa ggttttttcg cctgccggag agtgcgggat gtcgagcagc
    28981 atactgaagc ctttgtagga ctggttgtcc aaaagggcat tggtaaacgg ctcgttgttg
    29041 agcgagcaga tggagaacat ggtcgagccc gacgtgtcgg tatagtggaa cgcgccgtcg
    29101 tcttccaaaa cgaaacccac gcccgttacg gcggaacgca gttctacgcc gctgatgctg
    29161 gtcggcgaaa ccaaatggat ggcgatggtc tggtcgacgc gcgcgcagaa tgcgtccagt
    29221 gcggaagcca cttcgataaa ggcggcaagg tcggtgtgca gcgtctgacc gcccatgctt
    29281 tgtgcgaatg cgtccgcctg gcggttgaat gcggagagtt cttcctgcga ggcaagtccg
    29341 ttgcggctga ctgcctggat acccacgata aatgcctgat agcggatgcc cgggatgggt
    29401 tcggcaatct ggaaatggtc gtccatggtg cagccgacaa tctggtagcg gcagcggttg
    29461 gaaaggcgcg gcagtgcgtg cagttctttg gcttcggtca gcgcgatata ggagatgaag
    29521 tcgaagcgca cgtcaaacca gggcagctcg accttcgaca gctctttgag cgtaatcagc
    29581 ggttttgccg gcgtttgcgg aacgggaacg ggttttgccg gcgcgtcggc aggtttcggt
    29641 gcggaatgtc cggtttgggg ttcggaaacg gtgtgggcgg agttgccgat aatgccgatt
    29701 tcttccaagg cggtttcgat ttcggttttg aacggggagg cttttgcctg tttctgcttg
    29761 gcgatgtaga cggcatcctg ttcttgcagg ttgcgcatgg cggagtcttg gggtttggcc
    29821 ggttttttga ccgccggttg gggtttcggc atcatgactg gcccgccgga cggtttgccg
    29881 tcgcggacat ggctggtttt gctgttgagc agggcatctt tgtcggagtg tccgaactgg
    29941 tcgcgcactt ttttgcggta ttggttttcc tgatacatat tgtaggcgac aacggcgagg
    30001 acggcggcga ggaacagtac gatgtaaatc atggcaatca ctttaaattt cgggatgcag
    30061 gatgcgcaaa gtgcgggtac tgcggttaaa tcgggcttgc gtttccggca gtctgacgga
    30121 acggccggtt ataacgtttg aattataacg aaaattgcag ggtctgacag cagtgtgtcg
    30181 aaataagcgg aaattttccg aaatgccgtc tgaaatctgt ggttttcaga cggcatttct
    30241 gtccacggga aacccttttt cccgtatccg ccgccagtcg aaaaaatggc cggggtcggt
    30301 tttgcggccg ggtgcgatgt cttggtgtcc cgttaccgcc gtgatggggt agcggcggca
    30361 gagggctgcc aacagcgttt cgagcgaacg gtattgcgct tcggcaaacg gttcgaaatc
    30421 gcagccttcc agttcgatgc cgatggaaaa cgcgttgcac ttctcccgcc cccgaaacga
    30481 ggatgcgccg gcgtggtacg ccatgtcgcc gcatgatacg aactgcaccg tttcgccgtc
    30541 gcgtgcaatc aggaaatggc tggacacgtg caaagtgtgt atcaggctga agaacggatg
    30601 tccgtcgggg tcgagccggt tggcaaacag cttttccacc gcatccgtgc cgtattcgaa
    30661 cggcggcagc gaaatgttgt gcagcacgat cagggaaacc gtttctcccg tttccctcgg
    30721 gctgaaattg ggcgacgggg tatggcgtat gctttgaagc cagccgtttt gccagtgtgc
    30781 tccggcgtga ttgtccatga tgttcttcct gtccggcggg caatttgggt tatactgtcg
    30841 cccgaatttt aagacgtatt ccgaatgctg ggaatcctac catgttgaaa aaaatgttga
    30901 aatggactgc cgtttttttg accgtatcgg cagccgtttt cgccgcgctg ctttttgtcc
    30961 ctaaagacaa cggcagggca taccgaatca agattgccaa aaatcagggt atttcgtcgg
    31021 tcggcaggaa acttgccgaa gaccgcatcg tgttcagcag gcatgttttg acggcggcgg
    31081 cctacgtttt gggtgtgcac aacaggctgc atacggggac gtacagattg ccttcggaag
    31141 tgtctgcttg ggatatcttg cagaaaatgc gcggcggcag gccggattcg gttaccgtgc
    31201 agattatcga aggttcgcgt ttttcgcata tgaggaaagt catcgacgca acgcccgaca
    31261 tcggacacga caccaaaggc tggagcaatg aaaaactgat ggcggaagtt gcgcccgatg
    31321 ccttcagcgg caatcctgaa gggcagtttt ttcccgacag ctacgaaatc gatgcgggcg
    31381 gcagcgattt gcagatttac caaaccgcct acaaggcgat gcaacgccgc ctgaacgagg
    31441 catgggcagg caggcaggac gggctgcctt ataaaaaccc ttatgaaatg ctgattatgg
    31501 cgagcctgat cgaaaaggaa acggggcatg aggccgaccg cgaccatgtc gcttccgtct
    31561 tcgtcaaccg cctgaaaatc ggtatgcgcc tgcaaaccga cccgtccgtg atttacggca
    31621 tgggtgcggc atacaagggc aaaatccgta aagccgacct gcgccgcgac acgccgtaca
    31681 acacctatac gcgcggcggc ctgccgccaa ccccgattgc gctgcccggc aaggcggcac
    31741 tcgatgccgc cgcccacccg tccggcgaaa aatacctgta tttcgtgtcc aaaatggacg
    31801 gcacgggctt gagccagttc agccatgatt tgaccgaaca caacgccgcc gtccgcaaat
    31861 atattttgaa aaaataaacc atgccgtctg aaaagtttgt gttttcagac ggcataccct
    31921 taccggaact gcaagcatga aaccgcaatt catcactttg gacggcatag acggtgccgg
    31981 aaaatccacc aaccttgccg tcatcaaggc atggtttgaa cggagggggc tgcccgtgct
    32041 gttcacgcgc gagccgggcg gaacgccggt cggtgaggcc ttgcgcgaaa tcctgctcaa
    32101 ccctgaaacc aaagccggtt tgcgtgcgga aacactgatg atgttcgccg cgcgtatgca
    32161 gcacatcgag gaagtcatcc tgcccgcgct ttcagacggc atccatgtcg tgtccgaccg
    32221 ttttaccgat gcgaccttcg cctatcaggg cggcgggcgg gggatgccgt ctgaagacat
    32281 tgaaattttg gaacattggg tgcagggcgg tttgcgcccc gatttgaccc tgctgttgga
    32341 tgtgccgctg gaagtatcga tggcgcgtat cggacaggcg cgcgagaaag accggttcga
    32401 gcaggagcag gcggatttct ttatgcgtgt gcgcggcgtt tatctcgacc gagccgccgc
    32461 ctgtcccgaa cggtacgccg ttatcgacag taaccgcagc ttggatgaag tcagaaacag
    32521 catagaaaaa gtgttggacg gacatttcgg ctgttgatgc ggcaaatatt gaaacaagcg
    32581 catccgcccg cgccgaaaac caaacggcag tgccgcaggt gaaaatggcg gtatgcgcca
    32641 aactttcggc atgatagaat tacgctcggt tacaaggcag gatgcgtcgg caatattaac
    32701 gaaccgcccg taacatgatg acccgaaagc gtttcggaca gttcgattca aatctttttc
    32761 tcgcaacagg attgacacat ggaaaactca ttgaaagaag ccgccctcaa gttccacgaa
    32821 ttacccgttc cgggcaaaat ttccgttacc ccgaccaaat ctctggcgac cgacaaagat
    32881 ttggcgttgg cgtactctcc gggcgtagcc gccccctgta tggaaatcca tgccgatccg
    32941 caaaatgcct acaaatacac cggcaaaggc aacttggtcg ccgtcatttc caacggtacg
    33001 gccgttttgg gcttgggcga catcggcgcg ctggcgggca aacccgtgat ggaaggcaaa
    33061 ggcgtattgt tcaaaaaatt cgccggtgtg gacgtgttcg acatcgaaat cgacgaaaaa
    33121 gacccgcaaa aactggtgga catcatcgcc gctttggagc cgaccttcgg cggcatcaac
    33181 ctcgaagaca tcaaagcacc cgagtgtttc tacatcgaac gcgaattacg caaacgctgc
    33241 aaaatccccg tatttcatga tgaccagcac ggcacggcca tcattaccgc cgccgccgta
    33301 ttgaacgccc tgcgttatac cggccgtaaa atcgaagaag cgactttggt gtgctccggc
    33361 gcaggtgccg ccgcgattgc ctgcctgaac caactgctgg atttgggctt gaaacgtgaa
    33421 aacgtaaccg tttgcgactc caaaggcgtg atttaccaaa cccgcgaaga caaagaccgc
    33481 atggacgaat ccaaaaaatt ctacgccgtt gaagacaacg gccggcgcgt acttgccgat
    33541 gcggttaaag gcaaagacat cttcttgggc ctctccggcg caaacctgct gacgcctgaa
    33601 atgttgaaca cgatgaacga aaaacccatc gtgttcgcca tggccaaccc gaatccggaa
    33661 atcctgccgc cgctggcgaa agaaacccgt ccggacgtgg ttatcggtac cggccgctcc
    33721 gacttcccga accaagtgaa caacgtattg tgcttcccgt tcatcttccg gggcgcgttg
    33781 gatgtcggcg caaccaccat caacgaagaa atgaaacgcg cctgcgtgta tgctttggca
    33841 gatttggcga tggaagaagt aaccgaagaa gtggttgccg cttacggtaa gaaattcgaa
    33901 ttcggcgcgg aatacctgat tcctactccg ttcgattccc gcctgctgcc tcgcgtcgct
    33961 acggctgccg ccaaagcagc gatggaaagc ggcgtggcaa cccgtccgat tgcagatttg
    34021 gaagcttacg ctgccaagtt gggcgaatgg aagctgtaag ccgtttgtgg ttaaaatgcc
    34081 gtctgaactg ttttcaggcg gcattttgtt gtcagattga taaatgaaag atactggaaa
    34141 atgaaagaga tgaaacctgt ccgttatcat attggcgata tacccgaaac ttcaaaacaa
    34201 accgcccccg gtcatgacga cagggcagtg ggtgttgacg atgattgttt tcatgattcc
    34261 tttggtcaat atcgttatgt tttttgtttg ggcgttcggc agaggcaacc ccaaccgtgc
    34321 caatttctgt aaagcactgt tcttatttac tttaccttgt tggttcgctt atcggtttga
    34381 ttttcatatt gcttataggt gggtctgcat cgggtacgca ttattaatgt gccggctgat
    34441 tctgcttcga agatttgtat cgaatatgcc aaattttttt aaatttcata ccgttatcga
    34501 acggcattgg caaaaacctt atccggtttt gtcttttctg ctcaagccgc tttccgggct
    34561 gtttgccaaa attgcggcaa aatggcgggc ggatttttta tcgggaaaac ggcaaagcga
    34621 aaagctgtcc gtgcctgtgg tcgtggtcgg caacatccac gcgggcggga cgggcaaaac
    34681 gccgattgcc gccgcgctgg tgtcgggctt gcaggaaaaa ggtgtcaagg tcggcatcat
    34741 cagccgaggc tacgggcgca agagcaaggc ggtttatgta ttgaatgccg cgagccgtgc
    34801 ggaagatgcg ggcgacgagc ctttgctgct gttccgtaaa acaggtgcgc cgacggcagt
    34861 gggcagcagc cgtgtggagg caggcagggc gttgctggca gcgcatccgg agcttgaatt
    34921 gattgtggcg gacgacggtt tgcagcatta cgccctgcaa cgcgatgtgg aaatcgcggt
    34981 gtttccggcg gcggatacgg ggcgtacgga tttggacttg ctgcccaacg gcaatttgcg
    35041 cgaacctttg tcgagattgg aaagcgtgga tgcggtggtg gtcggcggca gggcggcgga
    35101 tggttttatg ccgtctgaac atttgttcgg cagccgtatt gaggcgggtg cggtttaccg
    35161 tttgaaccgt ccgtctgaaa aactggatat ttcgacattg tcagggaagc gcgtcgcagc
    35221 ggttgccggt atcgccaggc cgcagcgttt cttcgatact ctgacacaca tgggcatccg
    35281 ccttgaccaa acggttgctc tgcccgacca tgccgatatt ttcaatcggg atttgccgcc
    35341 tgccgatgtg gtgctggtaa ctgaaaaaga tgcggtcaaa ttttcagacg gcatttgcac
    35401 cgataatgtt tgggtgctgc cggtttgtgc gataatcgaa cctgatttgg cggagtttgt
    35461 gctggagcgg ttggagggtg taccgaaggc cgtctgaaag cgcggtttgg acgaagtgat
    35521 aacggactgg aataagaacg ccctacgccg tcattcccgc gcaggcggga atctaagtct
    35581 cgaattttca ggaatgccta ggagacttca gaaatcccaa atctccggat ttccacttgg
    35641 gcaggaatga gaaagccggt tgtatttttt atctgcatta atcattcgtt aaaggagtgg
    35701 atatgaagct gaaaaccttg ttattgccct tcgccgcact ggcattgtgt gccaacgcat
    35761 ttgccgcccc gcccggcgac gcgtcgttgg cacgttggct ggatacgcag aatttcgacc
    35821 gggatataga aaaaaatatg attgaaggct ttaatgccgg atttaaaccg tatgcggaca
    35881 aagcccttgc cgaaatgccg gaagcgaaaa aagatcaggc ggcagaagcc tttaatcgtt
    35941 atcgtgagaa tgttttgaaa gatttgatta cgcccgaagt gaaacaggct gtccgcaata
    36001 ccttattgaa gaatgcccgt gaaatataca cgcaagaaga aattgacggc atgattgcct
    36061 tttacggttc gcctgtcggt cagtccgtgg ttgccaaaaa tccgcgctta atcaagaaat
    36121 cgatgagtga aatagcggta tcttggactg cattgtcagg gaaaatcgcg cgacatcatc
    36181 tgcccgagtt tacggaagag ttacggcgca tcatctgcgg cggtatagtg gattaaattt
    36241 aaatcaggac aaggcggcga agccgcagac cgtacaaata gtacggcaag gcgaggcaac
    36301 gccgtaccgg tttaaattta atccactata aaaatcccga tgcgggctgc aagcaggccg
    36361 gacagcttgg gaaaaggtat cagaagtaaa taatagccgc ctgaaatatt gcggagggca
    36421 tccgattgat taaaccatca aacccgaaag caaccttatg gaaaaaaaat tcttagacat
    36481 cctcgtctgc cccgttacca aaggcaggct ggaatatcat caggacaaac aggaattgtg
    36541 gagccgtcag gcgaagcttg cctatccgat taaagacggc attccctata tgctggaaaa
    36601 cgaagcacga gcgttgagcg aagaggaact caaagcatga ccgaattcgt cgtattgatt
    36661 ccggcgcggc tggattcgtc gcgcctgccc ggaaaagcct tggcggacat tcacggcaaa
    36721 ccgatggtcg tgcgcgttgc cgaacaggcg gcaaaaagca aagccgcgcg cgtcgtcgtc
    36781 gccaccgatc atcccgacat tcagacggcc tgtcaggcgc acggtatcga agtcgtcatg
    36841 acttcaaacc ggcacgaaag cggcacgacg cgccttgccg aagccgccgc cgcgctgaag
    36901 ctgccgccgc atttgattgt tgtgaacgta cagggcgacg agccgctgat tgcccccgaa
    36961 ctcatcgacc gcaccgccga agtactcgtc gaaaacaacg tccaaatggc gaccgccggc
    37021 cacgaattgc acgatttcga cgaattgatg aatcccaatg ccgtcaaagt cgtcctcgac
    37081 aaaaacggca acgccatcta cttcagccgt gccccgattc cctatccgcg cgatgcgatg
    37141 cgtgccggaa aacgcgaaat gccgtctgaa accgccgtcc tacgccatat cggcatttac
    37201 gcctaccgcg tcggcttcct gcaacgctat gccgaaatga gcgtctcgcc gctggaaacc
    37261 atcgaatcgc tggaacaact gcgcgtcctg tggcacggct accccatcgc cgtcgaaacc
    37321 gccaaagaag cccccgccgc cggtgtggat acgcaggaag atttggacag ggttcgcgct
    37381 gtatttcaga ccgtataaaa caggttcaaa gggaaaagat atgcagcaac atattgaaaa
    37441 gtggcaacac ttgagccgtg aagaacagaa aatccttgcc gaagtatggg gtctcgtgca
    37501 aaacgatgat caggaggttc actatgaaat gctcaaattg aacgcacctg atgaagtcag
    37561 cggtgaattt tggttcagaa tggcagaaac actcagcacc ctgccgccca atcgttccct
    37621 cgaccttaga atgaacggcg gcaggctgtc gaccgccgta tccatccttt ccgtcatgat
    37681 tgaagacaat cccgacatac cgcagctttg ggcgtaaaaa attaccgccc tcaattattt
    37741 ggtacacgga cacaaagccc gtgccgacgg tttggtacaa cagcccgaca aagcggcaga
    37801 agccaacgag gaggaatacc tgaccaaagc cctgtcgcaa aacctgctgt caacattgga
    37861 tgccgcactt gcacgttttc ctgaagacgc gtggtttcag gaaatcaaac aggatgcaca
    37921 aaagcatttt gcttgaggac gtggcggtca ggaatatttc cattcaggaa gaaaagaagt
    37981 gcctaattgg gtataatcag ggtaaatctt attttatttc aaaagattaa tatttgtttt
    38041 ccgtttttcc ttgacggtat cggaaaagtt gattatagtt acagcttcct taggagtaat
    38101 ggctgagagg ctgaaggcac ttccctgcta aggaagcatg tgggatcaac ctgcatcgag
    38161 ggttcgaatc cctcttactc cgccagataa aaaaatagac gctgtgtttt acagcgtcta
    38221 tttttttatg caattttata gcgggttggc gcaaacccgg tatggtattg ccctgtcttg
    38281 attctgaatt ttgttaatcc gagatgtttg ccgtttattt ttgcctcgtt caaacggcgg
    38341 ctctgatctg cgcggtttct gtttgccgta ttcgcctacc cgtaccgcaa atgttatact
    38401 gggaaaaatt caccgattgt attttacggc gtatttgccg ataggatgga agagacaaat
    38461 gagcagaatc cggcaggctt ttgccgcttt ggatggcgga aaggcattga ttccctatat
    38521 tgcggtgggc gatcccgata ttcggacaac tttggcattg atgcacggca tggttgcaag
    38581 cggtgcggat attttggagt tgggcgtgcc gttttccgat ccgatggcgg acgggccggt
    38641 tattcagcgt gcggcggagc gggcgttggc aaacgggatt tcgctgcgcg atgtcttgga
    38701 tgtcgtcaga aaattccgtg aaaccgacac gcaaacgccg gttgttttga tgggatattt
    38761 gaatcccata cataagatgg gttatcggga gtttgctcag gaagccgcaa aggcgggggt
    38821 ggacggcgtg ttgacggtgg attcccccat cgaaaccatc gattctttat atcgcgagct
    38881 gaaggataac gaggtcgact gtattttcct gattgcgccg acgacgacgg aagaccgtat
    38941 taaaaccatt gccgagctgg caggcggatt tgtctattat gtttcgctca agggcgtaac
    39001 gggcgcggca agtttggata cggatgaggt ttcgcgtaaa atagagtatt tgcgtcagta
    39061 tatcgatatt cccatcggtg tcggtttcgg catcagcaat gcggaaagcg cgcgcaaaat
    39121 cggccgggtt gccgccgcga tcattgtcgg cagccggatt gtgaaagaga ttgaaaacaa
    39181 tgcgggcaac gaggctgccg ccgtcggtgc tttggtcaaa gagttaaagg atgccgtgcg
    39241 ctgattctgt cgcgcattct aaatgtttta ggagttgtcc atgagctggt tagataaaat
    39301 cctgccaccc aaaatcaaga atcgtgggaa agacggttct tccaatgttc ccgagggttt
    39361 gtggcgcaaa tgcccgtctt gttcggcaac cgtttattcg accgagttgc agcagaacaa
    39421 tcaggtctgc ccgaaatgca accaccacaa tccgttatcg gcgcgccagc gtttgaacct
    39481 gcttttggat gaggaaggca gggaggaagt tgcgggtaat gtcaaaccga ccgatccttt
    39541 gaagtttaaa gacagcaaaa aatatccgga ccgtttgagt gcggcacgca agctgaccgg
    39601 ggaagatgac gcgctggtgg tgatgaaggg gatgatgaac ggtctgcccg tcgtcgttgc
    39661 cgcgtttgaa ttccgcttta tcggcggttc gatgggttcg gttgtgggcg agcgtttcgt
    39721 acagggtgtc cgccgtgcgg ttgccgataa ttgtccgttt gtctgtgtgg cggcttccgg
    39781 cggcgcgcgt atgcaggagg gtgtaaactc gctgatgcag atgacgaaaa ccagtgccgc
    39841 gctgcatttg ctgacggaaa aacgcctgcc gtttatatcg gtgttgaccg atccgactat
    39901 gggcggcgta tctgccagct tcgcattttt aggcgatgtc gtgcttgccg aaccgaacgc
    39961 gctgatcggt tttgccggtc cgcgcgtgat tgagcagacg gtgcgcgaaa cgctgccgga
    40021 aggcttccaa cgcgccgagt ttctgctgga aaaaggcgcg atcgaccaga ttgtcgaccg
    40081 ccgcgatatg aagcggcgca tcagtgattt gattacgctg ttgtgccgtc aggacaaagt
    40141 ttccgccacc tgatggctga tgaatcgaat gccgtctgaa accgatgttt cagacggcat
    40201 ttttgtgtct ggttatttgt cgtgcggctt catcgacggg gcatagcgtc cggcacgttc
    40261 tttcaggcgt tgtaccaaac cttgcgtgtc ggcgggtacg ccgccctcgc agaatgcctg
    40321 atacaggacg gcgcgcaatg cgtcgttgcg cccaagtgtg ccgcctatcg gtttccattc
    40381 ggcgtttcgg ggctgtatcc agcggctgtt gaccgtatcg ccgtatccga atactttgta
    40441 ggaggaaagt ttgccgttgc cgaacccgat ggaggagagg gcgcagaata tgccttcggc
    40501 agtcaggttg tcgtagcctt tgtcggagcg gatattgaga atgtagcgga tgctgccgtc
    40561 gggcgcgggc ataatttgca ggctgtcgag caggattttc ggctgtttgc cgtaattttc
    40621 atccacataa atgtcaaacc agccgtccga gtgcgcatcg ggcagaggcg gcagttcggc
    40681 ggtatgttct ttaaattcgc gggcggcggc ttcttcgggc gtttcgcggt agcgggtgtt
    40741 gatcggcgtg tctttttggc tgaagccggc ggcaagggac gtgccgacag tcagggtcag
    40801 aatcagaagg atggcgcggc gcataagttt ctccaaattg aaaacggctt tattttatgg
    40861 tttggcggga agggctgcaa gcaatcgggg tataatctga cctgattttc attttaaagc
    40921 ggtgtcgaac catgaacagc gaaactttag acgtaaccgg attgaaatgt cccctgccga
    40981 ttttgcgggc aaaaaaggct ttggcgcaaa tgcggcaggg cgaggtattg accgttctgg
    41041 cgaccgacgg cggcgcgccg ggggactttg aggctttttg ccgccaaacc ggtcatgtgc
    41101 tgttggattc ttccgaacag gacggcgtgt tcacactggt cgtcaagcac aaataaatgc
    41161 cgtctgaaat gcggatgtcc tgattgatgt tattgctgtc ggcatttgtg cttgctgcgt
    41221 gtaatgccca aaaccggcaa tccggtgtac ggcatcagga ttttgcacgc gtagccgtcg
    41281 atttgtttcg gagtgcctgc gtcctgactc aaggtagttt cgaaccggtt tccaagtttg
    41341 ccgctgtcgg acattttgtc cctgtcggaa aggaagacct gtcccgtctg ccgcccgctg
    41401 ttgcagaacc cgatgtacag gcgttgtgga cgcttgaacg cgatggcggc atttattatt
    41461 tgagcctgac gcgcgacagt tgcagtgtga aagccgagcg ggcagacagt gccgcgcttt
    41521 tggaacattt ctcagggctt gtccgacagc cgccgaaaaa tgc
//
LOCUS       DAWTQS010000009        40512 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.009, whole genome shotgun sequence.
ACCESSION   DAWTQS010000009 DAWTQS010000000
VERSION     DAWTQS010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 40512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40512
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.009"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            95..475
                     /locus_tag="WNA25_000505"
     CDS             95..475
                     /locus_tag="WNA25_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFA9823332.1"
                     /translation="MPLKFQPRERSVIMCDFRGYEEPEMVKKRPVVVIARNRHNGKPV
                     TVVPLSSTEPVPLADCHHKMSENPLPDKPHIQCRAKCDMTATVGLARLDRYKPKGRDR
                     CIPIISEEDFQAVKTAVAKAFKLY"
     gene            complement(676..1518)
                     /locus_tag="WNA25_000506"
     CDS             complement(676..1518)
                     /locus_tag="WNA25_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823333.1"
                     /translation="MQNTISVFSFKSQNVRTQILGAEPWFCLGDVAEILQIQNARQLP
                     LKDQGIQKSSVATKKGNQELLFINEPNLYRVIFRSRKAEAVKFQDWIFEEVIPQIRKT
                     GGYQITPKTTADDRTGLRRAVAALVGRKRIGYSSAYSMIHQRFNVEAVEGIPADKLPE
                     AVAYVHALTLHTGLAGEVPDREPLPAPQPALPISGNALADIAAMVYYGTRMIELGKDV
                     SAPLKQLGCKQAVTMWTVWHETRSILKRSVAALEVLRGYADKDASGRIAACLEGIYGK
                     AAAR"
     gene            complement(2017..2193)
                     /locus_tag="WNA25_000507"
     CDS             complement(2017..2193)
                     /locus_tag="WNA25_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823334.1"
                     /translation="MNPTKQSKKSYESKRVLKHVSFNTEKEANLLEFSNNLDFSKWVK
                     EKLKHELELEKLKK"
     gene            complement(2521..3012)
                     /locus_tag="WNA25_000508"
     CDS             complement(2521..3012)
                     /locus_tag="WNA25_000508"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HFA9823335.1"
                     /translation="MRAVIQKTVGAKVDVVSEAGTETCGKIDGGFVVLLGVTHSDTEK
                     DARYIADKIAHLRVFEDEAGKLNLSLKDVGGAVLLVSQFTLYADAASGRRPSFSQAAP
                     AEQAQRLYLRTAELLRGHGIHVETGRFRTHMQVSLCNDGPVTILLDSFMTRISPKMKV
                     VPD"
     gene            complement(3086..3844)
                     /locus_tag="WNA25_000509"
     CDS             complement(3086..3844)
                     /locus_tag="WNA25_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFA9823336.1"
                     /translation="MKQKKTAAAVIAAMLAGFAAAKAPEIDPALVDTLVAQIMQQADR
                     HAEQSQRPDGQAIRNDAVRRLQTLEVLKNRALKEGLDKDKDVQNRFKIAEASFYAEEY
                     VRFLERSETVSESALRQFYERQIRMIKLQQVSFATEEEARQAQQLLLKGLSFEGLMKR
                     YPNDEQAFDGFIMAQQLPEPLASQFAGMNRGDVTRNPVKLGERYYLFKLGAVGKNPDA
                     QPFELVRNQLEQGLRQEKARLKIDALLEENGVKP"
     gene            complement(3914..4780)
                     /locus_tag="WNA25_000510"
     CDS             complement(3914..4780)
                     /locus_tag="WNA25_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFA9823337.1"
                     /translation="MKAKILTSVALLACSGSLFAQTLATVNGQKIDSSVIDAQVAAFR
                     AENSRAEDTPQLRQSLLENEVVNTVVAQEVKRLKLDRSAEFKDALAKLRAEAKKSGDD
                     KKPSFKTVWQAVKYGLNGEAYALHIAKTQPVSEQEVKAVYDNISGFYKGTQEVQLGEI
                     LTDKEENAKKAVADLKAKKGFDAVLKQYSLNDRTKRTGAPDGYVPLKDLEQGVPPLYQ
                     AIKDLKKGEFTATPLKNGDFYGVYYVNDSREVKVPSFDEMKGQIAGNLQAERIDRAVG
                     ALLGKANIKPAK"
     gene            complement(4829..5107)
                     /locus_tag="WNA25_000511"
     CDS             complement(4829..5107)
                     /locus_tag="WNA25_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HFA9823338.1"
                     /translation="MPAVDLIRERLQTLDPLVLEIGDESHLHKGHAGNTGGGHYAVLV
                     VSGRFEGLSRLNRQKTVKSLLKDLFSGGMIHALGIRAATPDEYFHTAD"
     gene            complement(5107..5397)
                     /locus_tag="WNA25_000512"
     CDS             complement(5107..5397)
                     /locus_tag="WNA25_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFA9823339.1"
                     /translation="MEYFMLLATDGEDVHEARMAARPEHFKRLETLKSEGRLLTAGPN
                     LLPDNPERVSGSLIVAQFESLDAAQAWAEDDPYVHAGVYSEVLIKPFKAVFK"
     gene            complement(5400..5930)
                     /locus_tag="WNA25_000513"
     CDS             complement(5400..5930)
                     /locus_tag="WNA25_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFA9823340.1"
                     /translation="MKFVSDLLSVILFFATYTVTKNMIAAAAVALVAGVVQAAFLYWK
                     HKRLDTMQWVGLVLIVVFGGATIVLGDSRFIMWKPTVLFWCGALFLLGSHLAGKNGLK
                     ASIGREIQLPDAVWGKLTYMWVGFLIFMGIANWFVFTRFEAQWVNYKMFGSTALMLFF
                     FIIQGIYLSTYLKKED"
     gene            6238..7263
                     /locus_tag="WNA25_000514"
     CDS             6238..7263
                     /locus_tag="WNA25_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFA9823341.1"
                     /translation="MKLIAASIAVAASFQAHARLGGLNIRSNLDEPFSGSITVTGEEA
                     KALLGGGSVTVSEKGLTAKVHKLDDKAVIAVSSAQAVRDPVLVFRIGAGAQVREYTAI
                     LDPVGCSPKTKSALSDGKTHRKTAPKAESQENQNAKALRKTDKKDSANSAVKPAHNGK
                     THTVRKGETLKQIAAAIRPKHLTLEQVADALLKANPNVSAHGRLRAGSVLHIPNLNRI
                     KAAAPKKIKAEQPKPQTAKPKAETASMPSEPSKQATVEKPIEKPVEKPVEKPEAKVAA
                     PEAKAEKPAVRPEPKPAVSETPASATERQPGPVPAANTAASETAAESAPPRSRRFCHR
                     HADRRNR"
     gene            7400..8554
                     /locus_tag="WNA25_000515"
     CDS             7400..8554
                     /locus_tag="WNA25_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV family protein"
                     /protein_id="HFA9823342.1"
                     /translation="MLLAGGGTALIALLLLLRLAQSKRARRTEESVPEEEPDLDDAAD
                     DGIKITFAEVETPATPEPAPKNDVNDTLALGGESEEELSAKQTFDVETDTPSNRIDLD
                     FDSLAAAQNGILSGALTQDEETQKRADADWNAIESTDSVYEPETFNPYNPVEIVIDTP
                     EPESVAQTAENKPETVDTDFYNNLFSNNHIGTEETASAKPAAPSGLAGFLKASSPETI
                     LEKTVAEVQTPEELHDFLKVYETGAVAETAPETPDFNAAADDLSALLQPAEAPAVEEN
                     AAEITLETPDSNTSEADALPDFLKDGEEETVDWSIYLSEENIPNNADTGFPSESVGSD
                     APSEAKYDLAEMHLEIGDRDAAAETVQKLLEEAEGDVLKRAQALAQELGI"
     gene            complement(8727..9143)
                     /gene="gloA"
                     /locus_tag="WNA25_000516"
     CDS             complement(8727..9143)
                     /gene="gloA"
                     /locus_tag="WNA25_000516"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFA9823343.1"
                     /translation="MRLLHTMLRVGNLEKSLDSYQNVLGMKLLRRKDYPEGRFTLAFV
                     GYGDETDSTVLELTHNWDTERYDLGDAYGHIAVEVDDAYEACERVKRQGGNVVREAGL
                     MKHGTTVIAFVEDPDGCKIEFVQKKSGDDSVAYANT"
     gene            complement(9168..10337)
                     /gene="lapB"
                     /locus_tag="WNA25_000517"
     CDS             complement(9168..10337)
                     /gene="lapB"
                     /locus_tag="WNA25_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFA9823344.1"
                     /translation="MDNELWIILLPIILLPVFFTMGWFAARVDMKTVLKQAKSIPSGF
                     YKSLDALVDRNSGRAARELAEVVDGRPQSYDLNLTLGKLYRQRGENDKAINIHRTMLD
                     SPDTVGEKRARVLFELAQNYQSAGLVDRAEQIFLGLQDGEMAREARQHLLNIYQQDRD
                     WEKAVETAQLLSHDEQTYQFEIAQFYCELAQAALFKSNFDAARFNVGKALEANKKCTR
                     ANMILGDIEHRQGNFPAAVEAYAAIEQQNHAYLSMVGEKLYEAYAAQGKPEEGLNRLT
                     GYMQTFPELDLINVVYEKSLLLKGEKEAAQTAVELVRRKPDLNGVYRLLGLKLSDLDP
                     AWKADADMMRSVIGRQLQRSVMYRCRNCHFKSQVFFWHCPACNKWQTFTPNKIEV"
     gene            complement(10348..10662)
                     /locus_tag="WNA25_000518"
     CDS             complement(10348..10662)
                     /locus_tag="WNA25_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HFA9823345.1"
                     /translation="MKLIYTVIKIIILLLFLLLAVINMDAVTFSYLPGQSVNLPLIVV
                     LFGAFVVGIVFGMFALFGRLLSLRGENSRLRAEVKKSARLSGQKLTAPPIQNAAESAK
                     QP"
     gene            10972..11970
                     /gene="ilvE"
                     /locus_tag="WNA25_000519"
     CDS             10972..11970
                     /gene="ilvE"
                     /locus_tag="WNA25_000519"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFA9823346.1"
                     /translation="MSRPVPAVFGSVFHSQMPVLAYREGKWQPTEWQSSQDLTLAPGA
                     HALHYGSECFEGLKAFRQADGKIVLFRPTANIARMRQSADILHLPRPETQAYLDALVE
                     LVKRAADEIPDAPAALYLRPTLIGTDPVIGKAGSPSETALLYILASPVGDYFKVGSPV
                     KILVETEHIRCAPHMGRVKCGGNYASAMHWLLKAKAEYGANQVLFCPNGDVQETGASN
                     FILINGDEIITKPLTDEFLHGVTRDSVLTVAKDLGYTVSERNFTVDELKAAVENGAEA
                     ILTGTAAVISPVTSFVIGGKEIEVKNQERGYAIRKAITDIQYGLAEDKYGWLVEVC"
     gene            complement(12215..13000)
                     /gene="fabI"
                     /locus_tag="WNA25_000520"
     CDS             complement(12215..13000)
                     /gene="fabI"
                     /locus_tag="WNA25_000520"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFA9823347.1"
                     /translation="MGFLQGKKILITGMISERSIAYGIAKACREQGAELAFTYVVDKL
                     EERVRKMAAELDSELVFRCDVASDDEINQVFADLGKHWDGLDGLVHSIGFAPKEALSG
                     DFLDSISREAFNTAHEISAYSLPALAKAARPMMRGRNSAIVALSYLGAVRAIPNYNVM
                     GMAKASLEAGIRFTAACLGKEGIRCNGISAGPIKTLAASGIADFGKLLGHVAAHNPLR
                     RNVTIEEVGNTAAFLLSDLSSGITGEITYVDGGYSINALSTEG"
     gene            complement(13155..13976)
                     /gene="dapD"
                     /locus_tag="WNA25_000521"
     CDS             complement(13155..13976)
                     /gene="dapD"
                     /locus_tag="WNA25_000521"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFA9823348.1"
                     /translation="MSLQNIIETAFENRADITPTTVAPEVKEAVLETIRQLDSGKLRV
                     AERLGVGEWKVNEWAKKAVLLSFRIQDNEVLNDGVNKYFDKVPTKFADWSEDEFKNAG
                     FRAVPGAVARRGSFVAKNAVLMPSYVNIGAYVDEGAMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQAAPTIIEDNCFIGARSEIVEGAIVEEGSVISMGVFIGQSTKIFDRT
                     TGEIYQGRVPAGSVVVSGSLPSKDGSHSLYCAVIVKRVDAQTRAKTSVNELLRGI"
     gene            complement(14186..15829)
                     /gene="pgi"
                     /locus_tag="WNA25_000522"
     CDS             complement(14186..15829)
                     /gene="pgi"
                     /locus_tag="WNA25_000522"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFA9823349.1"
                     /translation="MDAFTRAWYALERHYQDTCHILLRDRFAAEPDRFERMHERLDGM
                     LFDYSKNRFGEDTLQLLCRLAETADLEGKMRALRTGAKVNGSEGRAALHTALRLPDGA
                     DAVYADGRDVLPEIRRELNRALKFAHSLDDGLYQGITGKRIADFVHIGIGGSDLGPAM
                     CVQALEPFRRQISVHFVSNADPACLDEVLCRLNPETTMFCVASKSFKTPETLLNAEAV
                     KAWYRGAGFSESETARHFCAVSADTEAAQSFGIAAERVFAMYDWVGGRYSVWSPVGLP
                     VMVAVGGARFRELLAGAHAMDSHFFHTPPRRNIPVLMALIAVWYNNFQHADGQTAVPY
                     SHNLRLLPAWLNQLDMESLGKSRASDGSPAACKTGGIVFGGEGVNCQHAYFQLLHQGT
                     RLIPCDFIVPMTAQGAEDGRSRFTVANAFAQAEALMKGKTLDEARAELADLPEAERER
                     LAPHKEFPGNRPSNSILLDRLTPCNLGMLMAAYEHKTFVQGAIWNVNPFDQWGVEYGK
                     QLAKTIIGELEGGTSVHDASTEGLMAFYRECRLKGGGAA"
     gene            16339..17571
                     /gene="pilG"
                     /locus_tag="WNA25_000523"
     CDS             16339..17571
                     /gene="pilG"
                     /locus_tag="WNA25_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus assembly protein PilG"
                     /protein_id="HFA9823350.1"
                     /translation="MAKNGGFSLFAKKEKRFIFEGRHSASDKLVNGEVSAFTEEEARK
                     KLAKRGIRPLQITRVKTSSKRKITQEDITVFTRQLSTMIKAGLPLMQAFEIVARGHGN
                     PSMTEMLMEIRGQVEQGSSLSRAFSNHPKYFDRFYCNLVAAGETGGVLESLLDKLAIY
                     KEKTQAIRKKVKTALTYPVSVIAVAIGLVFVMMIFVLPAFKEVYANMGAELPPLTQTV
                     MDMSDFFVSYGWMVLIALGFAIYGFLKLKARSIKIQRRMDAILLRMPIFGDIVRKGTI
                     ARWGRTTATLFAAGVPLVDVLDSTAGAAGNLIYEEATREIRTRVIQGLSMTSGMRATE
                     LFPNMMLQMSSIGEESGSLDDMLNKAAEFYEDEVDNAVGRLSAMMEPIIIVILGLVIG
                     TLLVAMYLPLFNLGNVVA"
     gene            17645..18505
                     /locus_tag="WNA25_000524"
     CDS             17645..18505
                     /locus_tag="WNA25_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFA9823351.1"
                     /translation="MSDLSVLSPFAVPLAAVLGLLVGSFLNVVIYRVPVMMERGWTVF
                     AKEHLNLPLTDDESRTFNLMKPDSCCPKCRVPIRAWQNIPIVSYLLLRGKCASCQTKI
                     SIRYPLIELLTGVLFGLVAWQYGWSWITLGGLILTAFLISLTFIDADTQYLPDSMTLP
                     LIWLGLIFNLDGGFVPLQSAVLGAVAGYSSLWLLCAVYKLLTGKTGMGNGDFKLIAAL
                     GAWLGISALPVLIFVSSLIGLVAAIVMRVAKGRHFAFGPALTVSGWIIFTANDSVWRA
                     VNWWLTHPVR"
     gene            18502..19134
                     /locus_tag="WNA25_000525"
     CDS             18502..19134
                     /locus_tag="WNA25_000525"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFA9823352.1"
                     /translation="MTAWVGLTGGIGSGKSAAAQYFADLGVPRIDADAAAHSLTASDG
                     IALPEIRRLFGDTVFDTQGLLRRDILRKEIFASPSRKALLESVMLPLIFSEIKKQQET
                     FTDAVYGIVEIPLLTEKRQFISLIRRVLTISAPLEKRIGRVMARSGLTRGEVADIISH
                     QASESERLLLADDVLLNDGSLKSLREKTMLLHAFYSGIFASKPTQGKHNG"
     gene            19127..19336
                     /locus_tag="WNA25_000526"
     CDS             19127..19336
                     /locus_tag="WNA25_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFA9823353.1"
                     /translation="MAESRQTRLQVKCPTCQTAVVWKPENAFRPFCSQRCKLIDLGGW
                     ADGKYTVSGQTESLPEISEPDGAYR"
     gene            complement(19422..21098)
                     /gene="pilB"
                     /locus_tag="WNA25_000527"
     CDS             complement(19422..21098)
                     /gene="pilB"
                     /locus_tag="WNA25_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HFA9823354.1"
                     /translation="MSVGLLRILVQNQVVTVEQAEHYYNESQAGKEVLPMLFSDGVIS
                     PKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQMVEFHCVPVFRRGDKVFFAVSD
                     PTQMPQIQKTVSAAGIAVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
                     YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPI
                     AVRGQLASRIKVMSRLDISEKRIPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMR
                     ILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSGKTVSLYTCLNILNT
                     ESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETA
                     DIAIKAAQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSS
                     CKQEVERPSASALKEVGFTDEDLAKDWKLYRAVGCDRCRGQGYKGRAGVYEVMPISEE
                     MQRVIMNNGTEVGILDVAYKEGMVDLRRAGILKIMQGITSLEEVTANTND"
     gene            complement(21344..21415)
                     /locus_tag="WNA25_000528"
     tRNA            complement(21344..21415)
                     /locus_tag="WNA25_000528"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21380..21382),aa:Arg,seq:cct)
     gene            complement(21436..21882)
                     /locus_tag="WNA25_000529"
     CDS             complement(21436..21882)
                     /locus_tag="WNA25_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFA9823355.1"
                     /translation="MNFSFVPLFLVTLILLGVVSNNNSITVSATILLLMQQTALVQFV
                     PLVEKHGLNLGIILLTIGVLSPLVSGKAQVPPVAEFLNFKMISAVFIGIFVAWLAGCG
                     VPLMGRQPVLVTGLLIGTVIGVAFMGGIPVGPLIAADILSFVAGKV"
     gene            complement(21886..22860)
                     /locus_tag="WNA25_000530"
     CDS             complement(21886..22860)
                     /locus_tag="WNA25_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFA9823356.1"
                     /translation="MLENLPYFQRHLPEDLAKVNEVINRAVQSDVALISQIGTYIISA
                     GGKRLRPIMTILAGKAVGYDDEKLYSLAAMVEFIHTSTLLHDDVVDESDLRRGRATAN
                     NLFGNAAAVLVGDFLYTRAFQLMVASGSMRVLEVMADATNIIAEGEVMQLMNIGNTDI
                     TEEQYIRVIQYKTAKLFEAAAQVGAILGKASPGHEQALKDYGMYVGTAFQIIDDVLDY
                     SGETEETGKNVGDDLAEGKPTLPLIYLMRQGSEQVANDVRTALENADRGYFEKIRDYV
                     VRSDALAYSIGEARKAVDCAVAALDALSDSEVKDAMIQLAKESLVRVS"
     gene            complement(22876..23064)
                     /locus_tag="WNA25_000531"
     CDS             complement(22876..23064)
                     /locus_tag="WNA25_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823357.1"
                     /translation="MVNIRTPLCEIGHCIVICLFCQSLRGSGNHMPSEKMYPDGFADI
                     IARYLNQLSSGICRLTET"
     gene            23084..23392
                     /gene="rplU"
                     /locus_tag="WNA25_000532"
     CDS             23084..23392
                     /gene="rplU"
                     /locus_tag="WNA25_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFA9823358.1"
                     /translation="MYAVVKTGGKQYKVSVGEKLKVEQIPAQLDSQIELTEVLMIADG
                     ESVKVGAPFIEGAKVTAKVVAHGRGEKVRIFKMRRRKHYQKRQGHRQNFTQIEIVAIA
                     "
     gene            23417..23689
                     /gene="rpmA"
                     /locus_tag="WNA25_000533"
     CDS             23417..23689
                     /gene="rpmA"
                     /locus_tag="WNA25_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFA9823359.1"
                     /translation="MASKKAGGSTRNGRDSEAKRLGVKAYGNELIPAGSIIVRQRGTK
                     FHAGDNVGMGKDHTLFAKIDGYVEFKTKGALNRKTVSIRPYTGSEE"
     gene            23924..25108
                     /gene="ubiM"
                     /locus_tag="WNA25_000534"
     CDS             23924..25108
                     /gene="ubiM"
                     /locus_tag="WNA25_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-demethoxyubiquinol-8 5-hydroxylase UbiM"
                     /protein_id="HFA9823360.1"
                     /translation="MSLHSDILVVGAGPAGLSFAAELAGSGLKVTLIERSPLTVLQNP
                     PYDGREIALTHFSREIMQRLGMWDKIPENEIYPLRDAKVLNGRSDYQLHFPQPTEARG
                     EPADCLGYLISNHNIRRAAYEIVSQLDNVSILTDTAVKEVKTSDNEAQVFLENGDILT
                     ARLLLAADSRFSQTRRQLGISSDMHDYSRTMFVCRMKHTLSNQHTAYECFHYGRTIAL
                     LPLEKRLTNTVITVDTDKINSVQNLSPEELAASVKEQLKGRLGDMELVSSIHHYPLVG
                     MIAKRFYGKRSALIGDAAVGMHPVTAHGFNLGLSSADILAKLILEAEQRGQDIGAASL
                     LEKYSSKHMLHAHPLYHGTNMMLKLFTNETAPAKLLRGLVLRAGNNFPPLKKLITKQL
                     TG"
     gene            complement(25297..25452)
                     /gene="rpmG"
                     /locus_tag="WNA25_000535"
     CDS             complement(25297..25452)
                     /gene="rpmG"
                     /locus_tag="WNA25_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFA9823361.1"
                     /translation="MRDKIKLESGAGTGHFYTTTKNKRTMPGKLEIKKFDPVARKHVV
                     YKETKLK"
     gene            complement(25484..25717)
                     /gene="rpmB"
                     /locus_tag="WNA25_000536"
     CDS             complement(25484..25717)
                     /gene="rpmB"
                     /locus_tag="WNA25_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFA9823362.1"
                     /translation="MARVCKVTGKRPMSGNNVSHANNKTKRRFLPNLQSRRFWVESEN
                     RWVRLRVSNAALRTIDKVGIDVVLADLRARGEA"
     gene            complement(25967..27490)
                     /gene="farB"
                     /locus_tag="WNA25_000537"
     CDS             complement(25967..27490)
                     /gene="farB"
                     /locus_tag="WNA25_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     permease subunit FarB"
                     /protein_id="HFA9823363.1"
                     /translation="MDYPPLKGAALAWVTLSLGLAVFMEVLDTTIANVAVPVIAGNLG
                     AATTQGTWVITSFSVANAVSVPLTGFLAKRIGEVKLFTAAAAGFVIASWLCGIAPNLQ
                     SLVVFRILQGFIAGPLIPLSQSLLMASYPPAKRMLALALWAMTVVVAPVLGPILGGWI
                     SGNWHWGWIFFINIPIGIISAWITWKHLKHRETATVRTPTDYVGLTLMMVGIGALQMM
                     LDRGKELDWFASGEIITLGVVALVCLSYFIVWELGEKYPIVDLSLFKDRNFTVGVIAT
                     SLGFMVYMGTLTLLPLVLQTNLGYTSAWAGLAAAPVGILPVFLSPLIGRFGNKIDMRL
                     LVTASFLTFAFTFYWRTDFYADMDIGNVIWPQFWQGVGVAMFFLPLTTITLSHMKGGQ
                     IAAAGSLSNFLRVLMGGVGVSVVSTLWERREALHHTRFAEHITPYSATLHETAAHLSQ
                     QGISDGQTLGIINNTITQQGFIIGSNEIFLAGSILFIVLIPIVWLAKPPFHSGGGGH"
     gene            complement(27514..28668)
                     /gene="farA"
                     /locus_tag="WNA25_000538"
     CDS             complement(27514..28668)
                     /gene="farA"
                     /locus_tag="WNA25_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     adaptor subunit FarA"
                     /protein_id="HFA9823364.1"
                     /translation="METHTDETKLQNTQVKRKRRLTALTLLFALSAAAAGSAFFLWWQ
                     HEEETEDAYVAGRVVQVTPQKGGTVRKVLHDDTDAVKKGDVLAVLDDDNDVLAYERAK
                     NELVQAVRQNRRQNAATSQAGAQVALRRADLARAQDDLRRRSALAESGAVSAEELAHA
                     RTAVSQAQAAVKAALAEESSARAALGGDVSLREQPEVQTAIGRLKDAWLNLRRTQVRA
                     PADGQVAKRSVQVGQQVAAGAPLMAVVPLSDVWVDANFKETQLRHMKIGQPAELVSDL
                     YGKQIVYRGRVAGFSAGTGSAFSLIPAQNATGNWIKVVQRVPVRIVLNREDVDRHPLR
                     IGLSMTVKVDTSAAGAPVSKTPGAALPEMESTDWSEVDRTVDEILGQSAP"
     regulatory      28909..28953
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            28957..29430
                     /gene="queF"
                     /locus_tag="WNA25_000539"
     CDS             28957..29430
                     /gene="queF"
                     /locus_tag="WNA25_000539"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFA9823365.1"
                     /translation="MSRNNEELQGISLLGNQKTQYPTGYAPEILEAFDNKHPDNDYFV
                     KFVCPEFTSLCPMTGQPDFATIVIRYIPHIKMVESKSLKLYLFSFRNHGDFHEDCVNI
                     IMKDLIALMDPKYIEVFGEFTPRGGIAVHPFANYGKAGTEFEALARKRLFEHDAQ"
     gene            29505..30191
                     /locus_tag="WNA25_000540"
     CDS             29505..30191
                     /locus_tag="WNA25_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFA9823366.1"
                     /translation="MYALTAAQQQKALFRLVLFHILIIAASNYLVQFPFRIFGIHTTW
                     GAFSFPFIFLATDLTVRIFGSHLARRIIFWVMFPALLLSYVFSVLFHNGSWTGLGALS
                     QFNTFVGRIALASFAAYALGQILDIFVFDKLRRLKAWWIAPAASTVIGNALDTLVFFA
                     VAFYASSDEFMAANWQGIAFVDYLFKLTVCTLFFLPAYGVILNLLTKKLTALQTKQAQ
                     DRPVPSLQNP"
     gene            complement(30541..31833)
                     /locus_tag="WNA25_000541"
     CDS             complement(30541..31833)
                     /locus_tag="WNA25_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HFA9823367.1"
                     /translation="MAVFPLSAKHRKYALRALAVSIILVSAAYIASTEGTERVRPQRV
                     EQKLPPLSWGGSGVQTAYWVQEAVQPGDSLADVLARSGMARDEIARITEKYGGEADLR
                     HLRADQSVHVLVGGDGSAREVQFFTDEDGERNLVALEKKGGIWRRSASDADMKVLPTL
                     RSVVVKTSARGSLARAEVPVEIRESLSGIFAGRFSLDGLKEGDAVRLLYDSLYFHGQQ
                     VAAGDILAAEVVKGDTTHQAFYYRSDKEGGGGGNYYDEDGRVLQEKGGFNIEPLVYTR
                     ISSPFGYRMHPILHTWRLHTGIDYAAPQGTPVRASADGVITFKGRKGGYGNAVMIRHS
                     NGVETLYAHLSAFSQAQGNVRGGEVIGFVGSTGRSTGPHLHYEARINGQPVNPVSVAL
                     PTPELTQADKAAFAAQKQKADALLARLRGIPVTVSQSD"
     gene            31910..32878
                     /locus_tag="WNA25_000542"
     CDS             31910..32878
                     /locus_tag="WNA25_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823368.1"
                     /translation="MKLTLALPSLNLDEDEIRIPLCLPAFNKILQYGSPHRQSCTASA
                     FYARYLWCGRLAQRPAQSLNMPSETVALATPVWQKMGLHQANVLTAEYLDVGTDEAER
                     LCRDLSAFYGDIPWRFVPVLPELWLVSLPRAYRWGAKPVLDLGGLLGADDQPDGEDAL
                     EWLRVQTEIQMWLNAHPVNHNRKKRGLPELNGLWLWDSLHGSAQGGTLFADTVWSRFH
                     PNRRALPDSFRAYAETAAHLPDTHHILFMDDLRLTALTGDRERYAAILQQWEERWFAP
                     LYEAVRTGKIKRLDIATDGQHGGTLTFKPTDRRKFWRCTKTFDGIW"
     gene            complement(33079..34548)
                     /locus_tag="WNA25_000543"
     CDS             complement(33079..34548)
                     /locus_tag="WNA25_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HFA9823369.1"
                     /translation="MVIFYFYFCGKTFMPARNRWMLLPLLASAAYAEETPREPDLRSR
                     PEFRLHEAEVKPIDREKVPGQVREKGKVLQVDGETLLKNPELLSRAMYSAVVSNNIAG
                     IRVILPIYLQQARQDKMLALYAQGILAQAEGRVKEAVSHYRELIAAQPDAPAVRMRLA
                     AALFEDRQNEAAADQFDRLKTEDLPPQLMEQVELYRKALRERDAWKVNGGFSVTREHN
                     INQAPKQQQYGNWTFPKQVDGTAVNYRFGAEKKWSLKNGWYTTAGGDVSGRVYPGNKK
                     FNDMTAGVSGGIGFADRRKDVGLAVFHERRTYGNDAYSYANGARLYFNRWQTPRWQTL
                     SSAEWGRLKNTRRARSDNTHLQISNSLVFYRNARQYWTGGLDFYRERNPADRGDNFNR
                     YGLRFAWGQEWGGSGLSSLFRLGVAKRHYEKPGFFSSFKGERRRDKESDTSLSLWHRA
                     LHFKGITPRLTLSHRETWSNDVFNEYEKNRAFVEFNKTF"
     gene            complement(34790..35828)
                     /locus_tag="WNA25_000544"
                     /pseudo
     CDS             complement(34790..35828)
                     /locus_tag="WNA25_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581764.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(36168..36413)
                     /locus_tag="WNA25_000545"
     CDS             complement(36168..36413)
                     /locus_tag="WNA25_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823370.1"
                     /translation="MLKSIELNSHIRNRLAAYLKGRGLDFQTAMQEEEGNKEIAAIVH
                     SGLPTLVRKLYSEQKMQKFFWEKRDLIADYISRRMQG"
     gene            36525..36953
                     /locus_tag="WNA25_000546"
     CDS             36525..36953
                     /locus_tag="WNA25_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HFA9823371.1"
                     /translation="MMFSWFKLFHLFFVISWFAGLFYLPRIFVNMAMIDAPRGNPEYV
                     RLSGMAVRLYRFMSPLGFGAVVFGAAIPFAAGRWGSGWVHVKLCLGLMLLAYQLYCGV
                     LLRRFQDYSNAFSHRWYRVFNEIPVLLMVAALYLVVFKPF"
     gene            36965..37441
                     /locus_tag="WNA25_000547"
     CDS             36965..37441
                     /locus_tag="WNA25_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFA9823372.1"
                     /translation="MPIEIERRFLIENDKWRQYADEPLLLKQGYLSVEKERTIRIRIA
                     GKRAWLTLKGYISEISRSECEYEIPLADAEKMMETMCPFKMEKRRYPVRWGGSLFEVD
                     VFLGENSPLVVAEIELPAENADFDRPDWLGREITSDGMFTNAYLSKHPFSSWKNAV"
     gene            complement(37471..37959)
                     /locus_tag="WNA25_000548"
     CDS             complement(37471..37959)
                     /locus_tag="WNA25_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFA9823373.1"
                     /translation="MLKITIIAACAENLCIGAGNAMPWHIPEDFAFFKAYTLGKPVIM
                     GRKTWESLPVKPLPGRRNIVISRQADYCAAGAETVASLEAALALCAGAEEAVIMGGAQ
                     IYGQAMPLATDLRITEVDLSVEGDAFFPEIDRTHWKEAERTERRVSSKGVAYTFVHYL
                     KG"
     gene            complement(38027..39082)
                     /gene="aroG"
                     /locus_tag="WNA25_000549"
     CDS             complement(38027..39082)
                     /gene="aroG"
                     /locus_tag="WNA25_000549"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFA9823374.1"
                     /translation="MTHHYPTDDIKIKEVKELLPPIAHLYELPISKEASGLVHRTRQE
                     ISDLVHGRDRRLLVIIGPCSIHDPKAALEYAERLLKLRKRYENELLIVMRVYFEKPRT
                     TVGWKGLINDPHLDGTFDINFGLRQARSLLLSLNNMGMPASTEFLDMITPQYYADLIS
                     WGAIGARTTESQVHRELASGLSCPVGFKNGTDGNLKIAIDAIGAASHSHHFLSVTKAG
                     HSAIVHTGGNPDCHVILRGGKEPNYDAGHVSEAVEQLRAAGVTDKLMIDCSHANSRKD
                     YTRQMEVAQDIADQLEQDGGNIMGVMVESHLVEGRQDKPEVYGKSITDACIGWGATEE
                     LLALLAGANTKRMARAG"
     gene            complement(39244..39471)
                     /locus_tag="WNA25_000550"
     CDS             complement(39244..39471)
                     /locus_tag="WNA25_000550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823375.1"
                     /translation="MGNTRIPIKSFFKQLVLFVFNIFLAYEILPDVQAESAAGCPVPD
                     CRGNGKTDVPCGFARMNGGYCFYQSVSFSLK"
     gene            39407..39657
                     /locus_tag="WNA25_000551"
                     /pseudo
     CDS             39407..39657
                     /locus_tag="WNA25_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703192.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            39699..39995
                     /locus_tag="WNA25_000552"
     CDS             39699..39995
                     /locus_tag="WNA25_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823376.1"
                     /translation="MRFKAEYARSKNDADDKRGNAHIANGAIGKMAAVYAGYTYTQPG
                     GESTRFRGGLERVQVKRYLGEKCCGYACPKSERFTLQADGEIRQNLGEGWFLKL"
     gene            39992..40231
                     /locus_tag="WNA25_000553"
     CDS             39992..40231
                     /locus_tag="WNA25_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823377.1"
                     /translation="MSGVAVYLRNKNKIAIASQDANLNSKGRFVSSGLNVGKQLTGSL
                     GVEFDPYYRHRAIRKSAEFVPNTTKTKTDSEKFNE"
BASE COUNT         9704 a        11406 c         9994 g         9408 t
ORIGIN      
        1 tattggaagg cttttttttg caaccgcctt acacaaagcg gttttttgtg taagaactgc
       61 tataatagcc gcctgtcatc gtcaggagcg gctaatgcct ttaaaattcc aaccaaggga
      121 acgttcagtt atcatgtgcg actttcgcgg ttatgaagaa ccggaaatgg tcaagaaacg
      181 ccctgtcgtc gtcatagcgc gaaacaggca caacggcaaa ccggtaacgg tcgtaccctt
      241 aagcagcaca gagcctgtcc ctttggcgga ctgccaccac aaaatgagtg aaaacccctt
      301 accggacaag ccgcacatcc aatgtcgggc aaaatgcgat atgacggcaa cagtcggatt
      361 ggcacgatta gaccgataca aacccaaagg gcgcgaccgt tgcatcccaa taatcagtga
      421 agaggatttt caggcggtta aaacagccgt tgccaaggca ttcaaactgt actagaataa
      481 aaccgttccc ttaaaggggc ttgcaagact gttccgaaat atgggcagcc gcgcacgggc
      541 gacaggcgat gacaagccgt ccgtgcgtgt gatggggcgc ggaatgcgcc ccttgtcgta
      601 tctgcaaacg cctacaaatc cccaatcatc ccttcaataa aaatgccgtc cgaaccttca
      661 ggcggcattt tccgtttacc ttgccgcagc cttgccgtaa atgccttcaa gacatgcggc
      721 tatgcggccg gaggcgtctt tgtccgcata tccccgcaac acttcgaggg ctgcgacgga
      781 tcttttcagg attgaacggg tttcgtgcca aaccgtccac atcgtaaccg cctgtttgca
      841 gccgagctgc ttcagcggcg cggaaacgtc tttgcccaat tcgatcatcc gtgtgccgta
      901 ataaaccata gcggcaatgt cggccaaagc gttgccgctt atgggcaggg cgggttgcgg
      961 cgcgggcaac ggttcgcggt cggggacttc gccggccaaa cccgtgtgca gtgtcaatgc
     1021 gtggacgtag gcgacggctt cgggcagttt gtcggcgggg atgccttcga cggcttcgac
     1081 gttgaagcgt tggtgtatca tactgtacgc ggaggagtag cctatgcgtt tgcgcccgac
     1141 aagcgcggca accgcccggc gcaatccggt acggtcgtcg gcggtggttt tgggagtaat
     1201 ttggtagccg cctgttttgc ggatttgagg gataacctct tcaaatatcc agtcttggaa
     1261 tttgacggct tcggctttgc gtgaacggaa gatgactcgg tagagattcg gctcgttgat
     1321 gaaaagtaat tcttggttgc ctttttttgt tgcaacgctg gatttttgaa tgccctggtc
     1381 ttttaatggc aactgacggg cattttgaat ttgaagaatt tccgcaacat cgccaaggca
     1441 aaaccaaggt tcagccccca agatttgagt acggacattt tgagatttga aagaaaatac
     1501 agaaatggtg ttttgcattt taagctccac gaaagtttga aagcctctct tcgaccaaga
     1561 agaggtaggc ggacgtttca ggttggtcga ctgctcgtgg aaacagcaca tccggagatg
     1621 tcccgaaagt ccgcccgttg gctttttcag caagggtgta tgtttaacat acacccttca
     1681 gggaactctt atcaaaacaa aagccgcaag cgcgacaata accacgaaag aaacaggcga
     1741 ccaaacctgt gaaccgaact ttactattat tccgctattg cgtcaatatt ttggggggaa
     1801 gcacggacgg agctttgttg aaagtttaaa acttggacag tattcatggt agaatgacct
     1861 ttctatttca tggtagatag acgcggaaag agagttgccg ctctgctttc cacctgccca
     1921 aatcaagtta cagcttggtt tgggcttctt ttttatctgc aatcagcaag tgttgaaatt
     1981 ataatttgta tctacgattc atgcaagcca tttctattat tttttcagtt tttctaattc
     2041 aagttcatgt ttcagctttt cttttaccca tttggagaag tctaaattat tggaaaattc
     2101 caataaatta gcttcttttt cagtgttaaa tgaaacgtgc tttaaaacgc gcttagattc
     2161 gtagcttttt ttggattgtt tggttgggtt cattcttccc ccttgttgaa aataatcgta
     2221 gatacatttt actcgattta aaatcatagg gcaacaaaaa aaccgccaag tttgcggctt
     2281 ggcggcggtc gggctatttt aagaactctt ttcggatttt ttctttaacc cattgggaaa
     2341 aactaatttt atttgcacct tatttgcacc ttttttataa atataattca aatatttgat
     2401 ttatatatta atttaattaa atattacatg accctgcata ggggcgacgg aaaggcggcg
     2461 cgggggaagg gtgtgtgtat tgtcgttcat tgtcgatatt tcatcattgc ggactcaatt
     2521 tcaatccgga acaaccttca tttttgggga aatccgcgtc atgaaagagt ccagcagtat
     2581 ggttaccggc ccgtcgttgc agagcgacac ctgcatatgc gtgcggaaac gccctgtttc
     2641 gacatgaatc ccgtgtccgc gtaacagctc cgccgttcgc aggtaaagcc gttgcgcctg
     2701 ttctgccggc gcggcttggg aaaacgaagg ccgccgcccg cttgccgcgt cggcataaag
     2761 cgtaaactgc gacaccagca gcaccgcgcc gccgacatct ttcaaagaca ggttcagctt
     2821 gcccgcttcg tcttcaaaca cgcgcaaatg tgcgattttg tcggcgatat agcgtgcatc
     2881 tttttctgtg tcgctatgcg ttacaccgag taacacgaca aacccgccgt cgattttgcc
     2941 gcaggtttcc gtgccggctt cggacacgac atccaccttt gcacctaccg ttttctgtat
     3001 gaccgcacgc ataagccgtc ctccttacct gaacgtgcaa tgccgtctga aaaggcttca
     3061 gacggcatcg gtatcggaaa tgccattacg gtttgacacc gttttcttcc aaaagggcat
     3121 cgattttcaa gcgggctttt tcctgcctca aaccttgttc caactggttt ctgaccaact
     3181 cgaaaggctg cgcgtcgggg tttttcccga ccgcgccgag tttgaacagg taatagcgtt
     3241 cgcccaattt gaccggattg cgggtaacgt cgccacggtt cataccggca aactgcgaag
     3301 ccagcggctc gggaagctgc tgcgccataa tgaaaccgtc gaacgcctgc tcgtcgttcg
     3361 gataacgctt catcagccct tcaaaagaca gccctttgag caggagctgc tgcgcctgac
     3421 gcgcctcctc ttcggttgcg aagctgacct gctgcaattt gatcatgcgg atttgccgct
     3481 cataaaactg acgcagtgcg ctttcggaaa ccgtttccga acgttccaga aaacggacgt
     3541 actcctcggc ataaaaagac gcttcggcga ttttaaagcg gttttggaca tccttatcct
     3601 tatccaaacc ttccttcaat gccctgtttt tcaaaacttc caaagtttgc agccggcgga
     3661 cggcatcgtt tcggattgcc tgcccgtccg gtctttggga ctgctccgca tgccggtctg
     3721 cctgctgcat gatctgcgcc accagcgtat ccaccaaagc cgggtcgatt tcgggtgctt
     3781 tggctgccgc aaaacctgcc aacattgcag caataactgc ggcagcggtc tttttctgtt
     3841 tcatggtatt ccctgtcctg cgcggcggca aaacgcctgt atgaacgtct tgccgccgta
     3901 tcccgttttc agattatttt gcaggtttga tgtttgcctt acccaacagc gcaccgacgg
     3961 cacggtcaat ccgttccgcc tgaaggttgc cggcaatctg tcctttcatt tcgtcaaaag
     4021 aaggcacttt cacctcgcgg ctgtcgttga cataataaac gccgtagaaa tcgccgtttt
     4081 tcagcggcgt tgccgtaaat tcgccttttt tcaagtcctt aattgcctga taaagcggcg
     4141 gaacaccctg ttccaaatct ttcagcggca catatccgtc cggcgcgccg gtccgtttgg
     4201 tgcggtcgtt gagcgagtat tgtttcaaaa cggcatcaaa accttttttc gccttcaaat
     4261 cggcaaccgc ttttttcgca ttttcttcct tgtcggtcag gatttcgccc aactggactt
     4321 cctgcgtgcc tttataaaaa ccgctgatat tgtcgtaaac ggcttttact tcctgctcgg
     4381 aaaccggttg ggttttggcg atatgcagtg cgtatgcctc gccgttcaag ccatatttta
     4441 ccgcctgcca aacggttttg aaggacggtt tcttgtcgtc gcccgacttt ttcgcttcgg
     4501 cacgcaattt ggcaagcgca tctttaaact ctgccgaccg gtcgagtttc aggcgtttca
     4561 cttcctgtgc gaccacggtg ttgaccactt cgttttccag cagggattgg cgcagttgcg
     4621 gcgtgtcttc ggcacggctg ttttccgcac ggaatgcggc aacctgcgca tcgatgacgg
     4681 aactgtcgat tttctgaccg ttaacggttg ccagcgtttg ggcaaacagg ctgccggaac
     4741 aggcaagcag cgcaacggaa gtcaggattt ttgctttcat aatcttctct ctttaaattt
     4801 taaattgaaa tattcaggca gacttcattc agtccgccgt atggaaatac tcgtcagggg
     4861 tagccgcccg gatgccgagc gcgtgaatca tgccgcctga aaataaatct ttgagcagcg
     4921 atttgaccgt tttctgacgg ttcagacggc ttaaaccttc aaaacggccg ctgacgacca
     4981 aaacggcata atgtccgccg ccggtattgc ccgcgtgtcc tttgtgcaga tggctctcat
     5041 cgccgatttc caacaccaga ggatcgagcg tctgcaggcg ttcgcggatc aaatcgacag
     5101 ccggcattat ttgaacaccg ctttaaacgg cttgatcagc acttcgctgt acacgccggc
     5161 atgaacatag ggatcgtctt cagcccaagc ctgcgccgca tccaaagact cgaactgtgc
     5221 cacaatcaag ctgcccgaaa cacgttcggg attgtccggc agcaggtttg ggcctgccgt
     5281 cagcagacgg ccttccgatt tcagcgtttc cagccgttta aagtgttcgg gacgtgccgc
     5341 catacgcgct tcatgcacat cctccccgtc tgttgccagc aacataaaat attccacagt
     5401 caatcctcct ttttcagata ggtactcaga taaataccct gaataataaa gaaaaaaagc
     5461 atcagcgcag tcgaaccgaa catcttatag ttaacccatt gcgcttcaaa cctagtaaac
     5521 acaaaccagt tggcaatacc cataaaaatc agaaaaccga cccacatata tgtcaatttt
     5581 ccccatacgg catccggaag ctgaatctcc ctgccgatac tcgctttcaa gccgttttta
     5641 cccgcaaggt ggctgcccag caggaataac gccccgcacc agaacaatac tgtcggcttc
     5701 cacataatga agcggctgtc gcccaaaaca atggttgcgc cgccgaatac gacaatcagc
     5761 accagtccga cccactgcat cgtatccagc cttttatgct tccaatacag gaaagccgcc
     5821 tgaaccacgc ctgcaaccaa ggcaaccgcc gcagcggcaa tcatattttt ggtaacggta
     5881 taagtagcaa aaaacaagat gacggacaaa aggtcgctga caaatttcat atccaaatga
     5941 gaaagcaaat caaataaacg ctaatcatac aggcagaacc ctatctttgc acctgcaaaa
     6001 ccaacaaatt ttgttttgat taatgcaaat atgcacagat gtttttgaaa aaagatggaa
     6061 atatgtcata atttataaaa ttggctttaa aatacagctt cggtcggaac ctctgcctat
     6121 ccgcacccta tcgtccgtat tgcctatgcc tgatgacgaa atttacagac tgtacgcggt
     6181 caaaccgtat tcagccgcca acccacaggg gatacatctt gcaaaaacaa cagacaaatc
     6241 aaactgattg ccgcctccat cgcagttgcc gcatcctttc aggcacatgc gcgactgggc
     6301 ggactgaata tccggtccaa ccttgacgaa cccttttccg gcagcattac cgtaaccggc
     6361 gaagaagcca aagccctgct aggcggcggc agcgttaccg tttccgaaaa aggcctgacc
     6421 gccaaagtcc acaagttgga cgataaagcc gtcattgccg tttcttccgc acaggcagtc
     6481 cgcgatcccg tcttggtgtt ccgcatcggt gcaggcgcgc aggtacgcga atacaccgcc
     6541 atcctcgatc ctgtcggctg ctcgcccaaa accaaatctg cactttcaga cggcaagaca
     6601 caccgcaaaa ccgctccgaa agcagagtcc caagaaaatc aaaacgccaa agccctccgc
     6661 aaaaccgata aaaaagacag cgcaaactca gccgtcaaac cggcgcataa cggcaaaacc
     6721 cataccgtcc gcaaaggcga aacgctcaaa cagattgccg ccgccatccg tccgaaacac
     6781 ctgacgctcg aacaggttgc cgatgcgctg ctgaaggcaa acccaaatgt ttccgcacac
     6841 ggcagactgc gtgcgggcag cgtgcttcac attccgaatc tgaacaggat caaagcggca
     6901 gcacccaaaa aaatcaaagc ggaacaaccc aaaccgcaaa cggcgaaacc caaagccgaa
     6961 actgcatcca tgccgtctga accgtccaaa caggcaacgg tagagaaacc gatagagaaa
     7021 cctgtcgaaa aaccggttga aaaacctgaa gcaaaagttg ccgcgcccga agcaaaagcg
     7081 gaaaaaccgg ccgttcgacc cgaacccaaa cccgcagtgt ccgaaactcc ggcttcggcg
     7141 acagaacgcc aacccggacc tgtacccgct gcaaatactg ccgcatcgga aaccgctgcc
     7201 gaatccgccc ccccaagaag ccgccgcttc tgccatcgac acgccgaccg acgaaaccgg
     7261 taacaccgtt tccgaacctg ttgcggaaac agccagtgaa gttgccgccg aacccgttgc
     7321 cgaacctgtc gaacaggttt ctgccaaaga agaaaccgaa agcggactgt ttgacggtct
     7381 gttcggcggt tcgtacacct tgctgcttgc cggcggaggc acggcattga tcgccctgct
     7441 gctgctttta cgccttgccc aatccaaacg cgcgcgccgt accgaagaat ccgtccctga
     7501 agaagagcct gaccttgacg acgcggcaga cgacggcata aaaatcacct ttgccgaagt
     7561 cgaaactccg gcaacgcccg aacccgctcc gaaaaacgat gtaaacgaca cacttgcctt
     7621 aggtggggaa tctgaagaag agttatcggc aaaacaaacg ttcgatgtcg aaaccgatac
     7681 gccttccaac cgcatcgact tggatttcga cagcctggca gccgcgcaaa acggcatttt
     7741 gtccggcgca cttacgcagg atgaagaaac ccaaaaacgc gcggatgccg attggaacgc
     7801 catcgaatcc acagacagcg tgtacgagcc tgagaccttc aacccgtaca accctgtcga
     7861 aatcgtcatc gacacgcccg aaccggaatc tgtcgcccaa actgccgaaa acaaaccgga
     7921 aaccgtcgat accgatttct acaacaacct gttctcaaac aaccatatcg gcacagaaga
     7981 aacagcttcc gcaaaacctg ccgcaccctc cggactggca ggcttcctga aggcttcctc
     8041 gcccgaaacc atcttggaaa aaacagttgc cgaagtccaa acaccggaag agttgcacga
     8101 tttcctgaaa gtgtacgaaa ccggtgccgt cgcggaaact gcgcctgaaa cgcccgattt
     8161 caacgccgcc gcagacgatt tgtccgcatt gcttcaacct gccgaagcac cggccgttga
     8221 ggaaaatgca gcggaaatca ctttggaaac gcctgattcc aacacctctg aggcagacgc
     8281 tttgcccgac ttcctgaaag acggcgagga ggaaacggta gattggagca tctacctctc
     8341 ggaagaaaat atcccaaata atgcagatac cggtttccct tcggaatctg taggttctga
     8401 cgcgccttcc gaagcgaaat acgaccttgc cgaaatgcat ctcgaaatcg gcgaccgcga
     8461 tgccgctgcc gagacagtgc agaaattgct ggaagaagcg gaaggcgacg tactcaaacg
     8521 tgcccaagca ttggcgcagg aattgggtat ttgattccca actgcccttt cgcagatcaa
     8581 ggatgccgtt tcagacggca tctttttttg ccttatcggt gtaacggata aagtttgaac
     8641 cggcacaggc tcaaacagca ggccgacggc aacaaaatgc cgtccgaaac ccctaaaggc
     8701 ttcagacggc attggcggcg gcggtatcag gtattcgcat aggcaaccga atcgtcgccg
     8761 cttttctttt gaacgaactc gattttgcat ccgtcggggt cttctacgaa ggctatcacg
     8821 gttgtgccgt gtttcatcag gccggcttcg cggacgacgt ttccgccctg ccgcttcacg
     8881 cgttcgcagg cttcgtaggc atcgtccact tcaaccgcga tgtgtccgta ggcgtcgccc
     8941 aagtcgtatc gttccgtatc ccagttgtgc gtcagttcca aaaccgtgct gtcggtttcg
     9001 tcgccgtaac cgacgaaggc aagggtaaat ctgccttcgg gatagtcttt tcggcggagc
     9061 agtttcatac ccaaaacgtt ttggtaggaa tcgagggatt tttcgagatt gcccacgcgg
     9121 agcatagtat ggagtaagcg catttttcgt gttcctctcg gtggtggtta aacttcgatt
     9181 ttattcggcg taaacgtctg ccatttgttg caggcgggac agtgccagaa aaagacttgg
     9241 gatttgaagt ggcagttgcg gcaacggtac atcacgctgc gctggagctg ccgtccgata
     9301 accgaacgca tcatgtcggc atcggctttc caagccggat ccaaatcgct gagtttcaaa
     9361 ccgagcaggc ggtacacgcc gttaaggtcg ggcttgcggc ggacaagctc gacggcggtt
     9421 tgcgcggctt ctttctcgcc cttaagcagc agggatttct cgtacacgac attgatcagg
     9481 tcaagttcgg gaaacgtctg catatatcct gtcagacggt tcaagccttc ttcaggtttt
     9541 ccctgcgcgg cataggcttc gtaaagcttc tcgccgacca tgctcaagta tgcatggttt
     9601 tgctgctcga tggcggcata ggcttcgacg gcggcaggga aattgccttg tcggtgttca
     9661 atgtcgccca aaatcatgtt ggcgcgggtg cattttttgt tggcttcgag tgccttgccg
     9721 acattgaaac gcgcggcatc gaaattggac ttgaacagcg cggcttgggc aagttcgcaa
     9781 taaaactgtg caatctcaaa ctgatatgtc tgttcgtcgt gactaagaag ttgggcggtt
     9841 tcaaccgctt tttcccaatc cctgtcctgc tggtagatat tgagcaggtg ctgtctggct
     9901 tcacgcgcca tttcaccgtc ttgcagcccc aaaaaaatct gttcggcacg atcgaccaaa
     9961 cccgcgcttt ggtagttttg cgccaattca aacaggacgc gcgcgcgctt ttcgccgacc
    10021 gtatcgggag aatcgagcat tgtccggtgt atgttgatgg ctttgtcgtt ttcgccgcgc
    10081 tgacggtaaa gtttgccgag ggtaaggttc aaatcatacg attgcggccg gccgtcgacg
    10141 acttccgcca actcccttgc cgcgcgcccg ctgttgcggt cgaccaaagc gtccaggctt
    10201 ttataaaatc ccgaagggat gctttttgcc tgcttcaata cggttttcat atccacgcgg
    10261 gcggcaaacc agcccatcgt gaagaagacg ggcaaaagga taatcggcag caggataatc
    10321 cacaattcgt tgtccatatc ggctttctta aggctgtttg gcagattcgg cagcattttg
    10381 tatcggcggt gcagtcaatt tctgtccgct caagcgcgca cttttcttca cttccgcacg
    10441 caggcggctg ttttcgccgc gcaaggacag cagccgcccg aacagggcaa acattccgaa
    10501 cacgatgccg acgacaaacg cgccgaacaa tacgacaatc agcggcagat tgacactctg
    10561 ccccggaaga taggaaaagg taacggcatc catattaatg acggcaagca gcaggaagag
    10621 cagcaggata atgattttga tgacggtata gataagtttc atgtttgctc cgtaaataaa
    10681 aacgccgcac ggacagcttc gcactctgaa ccaagattgc gttagtttaa acgatttgca
    10741 cggtttcgga tagaatgcgg cagcgtgttc ggacggcata cggagtatgc gattgccgac
    10801 aattttacca aatacaccgc tcctttccat ttgaaaaata acggattgga caccgcatcg
    10861 acagaaaaac ccgccgcgca cttgtcaaaa ccctgtttgc aggcgtatct ttacaacctt
    10921 caaattcaaa ccgttcattg aaacatatca gaataagaaa ggctttacat catgagcaga
    10981 cccgtacccg ccgtattcgg cagcgttttt cacagtcaaa tgcccgtcct cgcctaccgc
    11041 gaaggcaaat ggcagccgac cgaatggcaa tcttcccaag accttaccct tgcacccggc
    11101 gcgcatgccc tgcattacgg cagcgaatgc tttgaaggtt tgaaggcctt ccgtcaggca
    11161 gacggtaaaa tcgtgctgtt ccgtccgacc gccaatatcg cgcgtatgcg gcaaagtgcg
    11221 gacattttgc acctgccgcg ccccgaaacc caagcttatc tcgatgcttt ggtcgaactg
    11281 gtcaaacgtg ccgccgacga aattcccgat gcgcctgccg cgctgtacct gcgcccgacc
    11341 ttaatcggta ccgatcccgt tatcggcaag gccggttctc cttccgaaac cgccctgctg
    11401 tatattttgg cttcccccgt cggcgactat ttcaaagtcg gctcgcccgt gaaaatcttg
    11461 gtggaaaccg aacacatccg ctgcgccccg catatgggcc gcgtcaaatg cggcggcaac
    11521 tacgcttccg ccatgcactg gttgctgaag gcgaaagccg aatacggcgc aaatcaagtc
    11581 ctgttctgcc cgaacggcga cgttcaggaa accggcgcat ccaactttat cctgattaac
    11641 ggcgatgaaa tcattaccaa accgttgaca gacgaattcc tgcacggcgt tacccgcgat
    11701 tccgtgctga ctgtcgccaa agatttgggc tataccgtca gcgaacgtaa tttcacggtt
    11761 gacgaactga aagctgctgt ggaaaacggt gcggaagcga ttttgaccgg tacggccgcc
    11821 gtcatctcgc ccgttacttc cttcgtcatc ggcggcaaag aaatcgaagt gaaaaaccaa
    11881 gaacgcggct atgccatccg taaggcgatt accgacatcc agtacggttt ggcggaagac
    11941 aaatacggct ggctggtcga agtgtgctga tgctttaaat aaaaaaatgc cgtctgaaac
    12001 ccgtttggcg tttcggacgg cattttcgcg ttcggaccgt ttccgccgca cctgcagcaa
    12061 gtcggtacaa aggcaatcgg ttaaaacaag cgtccgcatt tcccatcccg cctgccgcaa
    12121 gtcgggcatt tccctggaaa tacgcttcaa acggcaaacc gccgcccgaa accgatactc
    12181 ggcacggacg gcgcacggat ttgaaaacgg cggattatcc ctcggtgctc aaggcattga
    12241 tgctgtaacc gccgtcaaca taagtgattt cgccggtaat gccggacgac aggtcggaca
    12301 gcaggaaggc ggcagtattc ccgacttctt cgatggtaac gttgcggcgc agcgggttgt
    12361 gggcggcgac gtgtcccaag agtttgccga aatcggcgat gccggaggcg gcgagcgttt
    12421 tgatcgggcc ggcggaaatg ccgttgcagc ggatgccctc tttacccaga caggcggcgg
    12481 taaagcggat gcctgcctca aggctggctt ttgccatacc catcacgttg taattcggaa
    12541 tcgcgcgtac cgcgcccaag tagctaaggg cgacgatggc ggagtttctg ccgcgcatca
    12601 tcggacgggc ggcttttgcc aatgcgggca ggctgtatgc ggaaatttcg tgtgcggtgt
    12661 tgaacgcttc gcggctgatg ctgtcgagga agtcgccgct caaggcttct ttcggcgcga
    12721 agccgatgga atgcaccaaa ccgtccaagc catcccaatg tttgcctaag tcggcgaaca
    12781 cttggttgat ttcgtcgtcg ctggcgacat cgcagcggaa tacgagttcg gaatctaatt
    12841 ccgccgccat tttgcggacg cgctcttcca gtttgtccac aacgtaggta aacgccagtt
    12901 ccgcgccctg ttcgcggcag gctttggcga tgccgtaagc gatggaacgc tcggaaatca
    12961 tgccggtaat cagaattttt ttgccttgca gaaaacccat tttgtatcct tcaaatagtg
    13021 ttcggttcgt tctaaaccgt tgattatagc aaattgtccc tgtttctgtg ttttcacgtt
    13081 gcagcgtgca gacggcaatg ccgtccgaag cggatttcag acggcattgg acgtttcaaa
    13141 tacggtttaa ggcatcagat gccgcgcaag agttcgttca cgctggtttt ggcgcgggtt
    13201 tgcgcgtcca cgcgtttgac gatgacggca cagtaaaggc tgtggctgcc gtctttggaa
    13261 ggcaggctgc cggacacgac gaccgaacct gccggtacgc ggccttggta gatttcgcct
    13321 gtggtgcggt cgaagatttt ggtggattga ccgatgaaca cgcccatcga aatcacgctg
    13381 ccttcttcga caatcgcgcc ttcgacgatt tcggaacgcg cgccgatgaa gcagttgtct
    13441 tcaatgatgg tgggtgcggc ctgcaggggc tcgagtacgc cgccgatgcc gacaccgccg
    13501 ctcaagtgga cgtttttgcc gatttgcgcg caagagccga cggtcgccca agtatcgacc
    13561 atcgcgcctt cgtcgacgta tgcgccgatg ttgacgtaag acggcatcag cacggcgttt
    13621 ttggcaacaa agctgccgcg tcgggcaacc gcacccggaa ctgcgcggaa gcctgcgttt
    13681 ttgaactcgt cttcagacca gtcggcaaat ttggtcggca ctttgtcgaa gtatttgttc
    13741 acgccgtcgt tgaggacttc gttgtcttgg atgcggaagg acagcaacac ggcttttttc
    13801 gcccattcgt tgactttcca ctcgcccacg cccaagcgtt cggcgacgcg cagtttgcct
    13861 gaatcgagtt ggcggatggt ttccaacacg gcttctttga cttcgggggc aacggtggtc
    13921 ggagtgatgt ccgcgcggtt ttcaaaggcg gtttcgataa tgttttgcaa agacataatg
    13981 tttccttggt tcgagatatt tcgggacgct tgccggtatt gttcagacgg catggcggtt
    14041 ggaacaaacg tgctattgtg ccatacaaac gcccgcccgt cagccgcgcc gcaggcttgc
    14101 cggatgccgg cgtttcggaa tcgggcgggc ggcaggcata tgtctttttc cgcgcccgaa
    14161 tcaatacaga aaggcggcgg tacttttatg ccgcgccgcc gcctttcaga cggcattcgc
    14221 ggtaaaacgc catcagccct tcggtcgagg catcgtgtac ggacgtgccg ccttccagtt
    14281 cgccgatgat ggtttttgcc aactgtttgc cgtactcaac cccccactga tcgaaggggt
    14341 tgacgttcca tatcgcgcct tggacgaagg ttttgtgttc gtaagccgcc atcagcatac
    14401 ccagattgca gggcgtgagg cggtcgagca ggatgctgtt gctggggcgg ttgccgggga
    14461 actctttgtg cggcgcgagg cgttcgcgtt ccgcttcggg caaatctgcc agttcggcgc
    14521 gtgcttcgtc taaggtttta cccttcatca gggcttccgc ttgggcaaag gcgttggcaa
    14581 cggtaaaacg gctgcgtccg tcctccgcgc cctgcgccgt catcgggacg ataaagtcgc
    14641 agggaatcag gcgcgtgcct tggtggagca gttggaaata ggcgtgctgg cagttgaccc
    14701 cttcaccgcc gaacacgatg ccgcccgttt tgcacgcggc gggactgccg tctgaagcgc
    14761 ggcttttgcc caaactctcc atatcgagct ggttcagcca cgccggcagc aggcgcaggt
    14821 tgtggctgta cggaacggcg gtctgcccgt ccgcgtgctg gaaattgtta taccacacgg
    14881 caatcagtgc catcagaacg ggaatgttgc gacgcggggg cgtgtggaaa aaatggctgt
    14941 ccatcgcgtg cgcccccgct agcagttcgc ggaaacgcgc cccgccaacc gcaaccatca
    15001 cgggcaaacc gacgggcgac cagacggaat agcgtccgcc cacccagtcg tacatcgcaa
    15061 acacgcgttc cgccgcaatg ccgaaacttt gcgccgcttc cgtatcggca gacaccgcgc
    15121 aaaaatggcg cgccgtttcg gattccgaga accccgcacc gcgataccac gccttgaccg
    15181 cctctgcatt gagcagggtt tccggtgttt taaaggactt gctggcaacg caaaacattg
    15241 ttgtttcggg gttcagacgg cataagacct catccaggca ggcagggtcg gcattagaaa
    15301 caaaatggac ggaaatctgc cgtctgaacg gctcgagcgc ctgcacgcac attgccggcc
    15361 cgaggtcgga tccgcctatg ccgatgtgga caaaatccgc aatccgtttt ccggttatcc
    15421 cctgatataa accgtcgtcc aaactgtgtg caaacttcaa cgcacgattt aactcgcggc
    15481 ggatttcggg caacacgtcc ctgccgtccg cataaacggc atccgcaccg tcgggcaggc
    15541 gcaaagccgt atgcagcgcg gcacgcccct cgctgccgtt gactttcgca cccgtccgca
    15601 aagcacgcat tttcccttcc aaatccgccg tctccgcaag acggcagagc agttgcagcg
    15661 tatcttcgcc aaaacggttt ttgctgtaat cgaacaacat cccgtccaaa cgctcatgca
    15721 tacgctcaaa acggtcgggt tcggcagcaa agcggtcgcg caaaaggatg tggcacgtat
    15781 cctgataatg gcgttcgagc gcataccatg cacgggtaaa ggcatccatc tgttttcctt
    15841 gatttttcag aaccggatta aaatatagca gaatgtagtc taacaaacgg cagacacttt
    15901 agcgaatctc aggaacaccg cccgccgcgt ccaataacac cttatttcca tatggcgaaa
    15961 atatagtgaa ttaacaaaaa tcaggacaag gcggcgggcc gcaggcagta cggatagtac
    16021 ggaaccggtt cgcttggtgc ttcatcacct tagggaatcg tcctctttga gccggggcgg
    16081 gccaacgccg taccggtttt tgttaattca ctataccctc cggccggcat ttgacccgca
    16141 acaatatcct gcaagattta ttgtgtgcgc ataaatgccg gacagccgcc taaattaagg
    16201 tataatcgca tccgattctg tcccgtttgc cgcccgcagg cgggatgtcg gcggttttca
    16261 tcttaccggg aacacgccga tactgttttt taacgatgtt tcttacattt tattccaatt
    16321 actttacggg gctagaatat ggctaaaaat gggggatttt ctttgttcgc aaagaaagaa
    16381 aaacgcttta tctttgaagg caggcattct gcctccgaca aactggtcaa cggcgaagta
    16441 tccgcgttta ccgaagaaga ggcacgcaaa aaactggcaa aacgcggcat ccgcccgttg
    16501 cagattaccc gtgtgaaaac aagctccaag cgcaaaatca cacaagaaga cattaccgtt
    16561 ttcacccgcc agctttccac gatgattaaa gcgggcctgc cgctgatgca ggcatttgaa
    16621 atcgtggcgc gcggacacgg caacccgtct atgacggaaa tgctgatgga aatccgaggc
    16681 caagtggaac agggcagctc gttgagccgc gcattctcaa accacccgaa atatttcgac
    16741 cgcttctact gcaacctggt tgcggcgggc gaaacgggcg gtgtattgga aagcctgctg
    16801 gacaaattgg caatttacaa ggaaaaaacc caggccatcc gcaaaaaggt aaaaaccgcg
    16861 ctaacctatc cggtatccgt catcgccgtc gccatcggtt tggtattcgt gatgatgatt
    16921 ttcgtactgc ccgcctttaa agaagtttac gccaatatgg gcgcggagct tcccccactg
    16981 acccaaacag tgatggatat gtccgacttt ttcgtctcat acggctggat ggtgctgatc
    17041 gcactgggct ttgccatata cggcttcctt aaattgaagg cgcgttcgat taaaatccaa
    17101 cggcgtatgg atgccatact gctgcgtatg ccgattttcg gagacattgt ccgcaaaggg
    17161 acgattgccc gctggggcag gacgacggca acgctgtttg ccgccggcgt gcctttggtc
    17221 gatgtattgg actccactgc cggcgcggcg ggcaatttaa tctatgaaga agccacccgg
    17281 gaaatccgta cgcgcgtcat ccaaggtctg tctatgactt cggggatgcg tgcgacggaa
    17341 ctgttcccca atatgatgtt gcagatgtcc tccatcggcg aggaatcggg ttctttggac
    17401 gatatgctca acaaagccgc cgaattttac gaagacgagg tggacaatgc ggtcggcagg
    17461 ctgtccgcta tgatggagcc gattattatt gtgattttgg gcttggtcat cggtacgctt
    17521 ctggtcgcta tgtatctgcc gctgttcaac ttgggcaacg tggtcgcctg atttgccgca
    17581 cagatccggc gcggattggt tctgcgccgg tttgtttttg ctttgaatat atcaaggaca
    17641 aaatatgtct gatttgtctg tattgtcgcc gtttgccgtg cctttggcag cagttttggg
    17701 gctgctggtc ggcagcttcc tgaatgtcgt catttaccgc gtacccgtta tgatggaacg
    17761 cggctggacg gtatttgcca aagaacattt aaacctgccg ctgaccgacg atgaaagccg
    17821 taccttcaac ctgatgaagc cggattcctg ctgtcccaaa tgccgtgtgc cgatacgcgc
    17881 gtggcagaac atcccgattg tcagttacct gctcctgcgc ggcaaatgcg cttcctgcca
    17941 aaccaaaatc agcatacgtt atcccttaat cgagctgctg accggcgtat tgttcgggct
    18001 ggtcgcctgg caatacggct ggtcttggat tacgctgggc ggtttgatac tgaccgcgtt
    18061 tctgatttcc ctgaccttta tcgatgcgga cacccaatac ctgcccgact cgatgacatt
    18121 acccttgatc tggctggggc tgatatttaa tttggacggc ggcttcgtgc ctttgcagtc
    18181 tgccgtttta ggtgcggttg ccggctatag ttcattatgg ctcttatgtg cagtgtataa
    18241 actgctcaca ggaaaaaccg gtatgggcaa cggagatttc aaactgattg ccgcattggg
    18301 cgcgtggctc ggcatatccg cattgcccgt gctgattttt gtttcctctc tgatcggttt
    18361 ggtcgcggca atcgttatgc gcgtcgccaa ggggcggcat tttgccttcg gccccgcact
    18421 gacagtttcg ggctggataa tttttacggc aaacgattcc gtatggcggg cggtcaactg
    18481 gtggctgacc catccggtga gatgacagca tgggtcggac tgaccggcgg aatcggcagc
    18541 ggcaaatcgg cagccgcgca atattttgcc gatttgggcg tgccgcgcat cgatgcggac
    18601 gcggcggcgc actcgctgac ggcttcagac ggcatcgccc tgccggaaat caggcggctg
    18661 ttcggcgaca ccgttttcga cacacagggt ttgttgcggc gcgacatatt gcgtaaagaa
    18721 atctttgcct ccccatcgcg caaagccttg ctcgaatccg tgatgttgcc gctgattttc
    18781 tcagaaatca aaaaacagca agaaaccttt accgatgcgg tttacggcat tgtcgaaatt
    18841 ccgctgctga cggagaagcg tcaatttatc agcctgatac ggcgtgtcct gaccataagc
    18901 gcacctttgg aaaaacgtat cggcagggta atggcacgca gcgggctgac gcgcggcgag
    18961 gtcgcggaca tcatcagcca tcaggcatcc gaatccgaac gcctgctgct tgcagacgat
    19021 gtactgctca atgacggcag cctcaaaagc ctacgtgaga aaacaatgct cctgcacgcg
    19081 ttttattcag ggattttcgc ctcaaaacca acacaaggaa aacacaatgg ctgaatcgcg
    19141 gcaaacacgc cttcaagtca aatgtccgac ctgtcaaacg gcagtagtat ggaaaccgga
    19201 aaacgcattc cgcccgttct gttcgcaacg ctgcaaactg atcgacttgg gcggatgggc
    19261 agacgggaaa tatacggttt ccggccaaac ggaaagtttg ccggaaatat ccgaacccga
    19321 cggggcatac cgctgaccgc cccgccttcc cggcaaacac cctgaaaggt caaatgccgt
    19381 ctgaaacaaa cacgcttcag acggcatttt cattctcaaa cctaatcgtt ggtatttgcc
    19441 gttacctctt ccaatgaagt aatgccctgc ataattttca aaataccggc ccggcgcaaa
    19501 tccaccatac cctccttata ggcaacgtcc aaaataccca cttccgtacc gttgttcata
    19561 atcacacgct gcatttcttc gctgatgggc ataacctcat acacgcccgc acgcccctta
    19621 taaccctgcc cccggcaacg gtcgcaaccg acggcgcggt aaagtttcca atcttttgca
    19681 agatcctcat cggtgaaacc gacttccttc aaagcagagg cagacgggcg ttccacttcc
    19741 tgtttgcagc tcgaacacag cctgcgtaaa agacgctgcg ccataatcag gctgaccgaa
    19801 ctggcaatat taaacggcgc gacacccata ttcagcatac gcgacaacgt cgccggcgca
    19861 ttattcgtgt gcagtgtgga aaacaccata tgccctgttt gtgccgcctt aatcgcaata
    19921 tcggcagttt ccaaatcacg aatctcaccg accataatga tgtccgggtc ctgacgcagg
    19981 aaagacttca aagcagcggc aaaagtcaga ccctgcttat cattgacgtt aacctgattg
    20041 atgcccggca ggttaatctc ggcagggtct tccgccgttg caatatttac cgactccgta
    20101 ttcaaaatat tcaaacaggt atagagcgac accgtcttac ccgaacccgt cgggccggtt
    20161 accagcacca tcccgtaagg acggtgaatc gcttccaaca acaatttttt ctggaacggc
    20221 tcaaaaccga gctggtcgat gttcaaagac gcggcatcgg aattcaaaat ccgcatcacg
    20281 accttttcgc caaacagcgt cggcaatgtg ctgacacgga aatcgacagg cttgccgccc
    20341 ttttgaaagg tcagctgcat cctaccgtcc tgcggtatcc gtttttcgga aatgtccaaa
    20401 cgcgacatta ccttaatccg ggaagcaagc tgccccctta ccgcaatggg cggctgaacc
    20461 acctcgcgga gctgcccgtc cacacggaaa cggatacgcg cattgtgttc gtaaaactcg
    20521 aaatggatgt cggatgcccc gctacgcaag gcatccgaca aagttttatg gataaacctc
    20581 ggaacagggc cgtcttctgc ctcctcgttg tcgatataca gggtgtggct ttcctcttcc
    20641 tcttgcccct ccccaagctc ctgaagcagc gatgtcgaac gcgaacccac ccaatcgagc
    20701 aaacccgcca actggtcatc ctcgacaatg accaactcaa ccgcaatccc tgcggcagaa
    20761 acggttttct gaatttgcgg catctgggtc ggatcggaaa ccgcaaaaaa tactttgtcg
    20821 ccccgacgga aaaccggcac acagtggaac tccaccatct gctcctccgt caacaccccc
    20881 atcagcaccc tgtggcgcgg ataatgacgc aaatcaagaa tcgaataact gaacaccctc
    20941 gcaatcaatg ccgcaagcga cttgggcgaa atgacaccgt ctgaaaacag catcggcaac
    21001 acttccttac ccgcctgcga ctcattgtag taatgctcgg cctgctcaac agtaaccacc
    21061 tggttttgaa ccagaatcct cagcaaacct acgctcatac gaccttatcc ccaaatttat
    21121 tcattgttat acctgtacag attttatcat aaaacaacag atttccaagc ctgaacatct
    21181 tttacgaagc ctgaaaatca tttcacgatt ctgccgtcct aaaggtcggt ttttcaagca
    21241 ggaagaaaaa gttttcagat ggcaaaaaag ccctccagca ctgaaaggcc ttatatcgga
    21301 aacttcccgc aacacgggaa acagacaaat gcaaaccgtc aaacctcgcc aacaggaatc
    21361 gaacctgtat tttacgctta ggaggcatac gttctatccg ttgaactatg gcgagccgaa
    21421 atgaaaaggg agattttaaa cctttccggc aacaaaagac aagatgtcgg ccgcaatcag
    21481 cggcccgaca gggataccgc ccataaatgc caccccgatg accgtaccga tcagcaagcc
    21541 cgttaccaat accggctgcc gtcccatcaa aggcacgccg catcccgcca gccaagccac
    21601 gaaaataccg ataaaaacgg cggatatcat tttaaaattc aaaaattcgg caacaggagg
    21661 aacctgcgcc tttcctgaaa ccaacggact caaaacccct atggtcaaaa gaatgatacc
    21721 gaggttcaac ccgtgcttct cgaccaacgg gacaaactgt accaatgccg tctgctgcat
    21781 cagcagcaat atggttgccg agacggtaat cgaattgttg ttgctgacca cccccaacag
    21841 aatcagcgta accagaaaca ggggaacaaa actgaaattc atcggttacg acaccctgac
    21901 caaagattcc ttcgccagct gaatcatagc atccttcact tcgctgtcgg acagggcatc
    21961 caacgcggca acggcacaat cgactgcttt gcgcgcctcg cctatcgaat atgccaaagc
    22021 atccgaacgg acgacataat cgcggatttt ttcaaaatag ccgcgatctg cattttccaa
    22081 agcagtacgc acatcgttcg caacctgttc ggaaccctga cgcatcagat aaatcaaagg
    22141 cagggtcggt tttccttccg ccaaatcgtc gccgacgttt ttacctgttt cttcggtttc
    22201 ccccgaataa tccagcacat cgtcaataat ctggaatgcc gtaccgacgt acataccgta
    22261 gtctttcaag gcctgttcgt gcccggggga agctttgccc aaaattgcgc cgacttgagc
    22321 ggcagcttca aacaattttg ccgttttata ttggatgact cggatatatt gttcttcggt
    22381 aatgtccgta ttgccgatgt tcatcagctg catgacctct ccctcggcaa tgatgttggt
    22441 cgcatccgcc atcacttcca aaacgcgcat actgcccgag gcaaccatca gttgaaatgc
    22501 acgggtatat aaaaagtcgc caaccaacac tgccgccgca ttgccgaaca gattgtttgc
    22561 cgttgcccgc ccacggcgca aatcgctttc atcgacgaca tcgtcgtgca ggagtgtgga
    22621 agtgtggata aactcgacca tcgctgccag cgaatacagt ttctcgtcat cataaccgac
    22681 cgccttaccc gccaaaatcg tcataatcgg acgcaggcgt ttgccgcccg cgctgatgat
    22741 atatgtaccg atttgcgaaa tcagtgcgac atcggattgc accgcacggt tgatgacttc
    22801 attgactttg gcaaggtctt caggcagatg tcgctggaaa tagggcagat tctcgagcat
    22861 ggcagactct caggtttacg tttccgtcaa acggcagata ccgcttgaaa gctgattcaa
    22921 atagcgggca attatatcag caaagccatc agggtacatc ttttcagacg gcatatgatt
    22981 gcccgaaccg cgcaaacttt gacaaaacag gcaaataaca atacaatgcc caatttcgca
    23041 caatggtgtg cggatattaa ccataaccct tatggagttg agtatgtacg cggtcgtaaa
    23101 aaccggcggc aaacagtata aagtttccgt cggcgaaaaa ttgaaagtag aacagatacc
    23161 agcccaactc gacagccaaa tcgaactgac cgaagttttg atgattgctg acggcgaatc
    23221 tgtaaaagtt ggcgcaccct ttatcgaagg tgcaaaagta acggctaaag tagtggcaca
    23281 cggtcgtggc gaaaaagtcc gcatcttcaa aatgcgccgc cgcaaacact accaaaaacg
    23341 ccaaggccac cgccaaaatt tcacccaaat cgaaatcgtg gcaatcgcct aaccttaaaa
    23401 cttaggagca ttacaaatgg caagtaaaaa agcaggcggc agcacccgca acggtcgcga
    23461 ttcagaagcc aaacgcttgg gcgttaaagc ctacggcaac gagctgattc ccgcaggttc
    23521 catcatcgta cgccaacgcg gtaccaaatt ccacgcaggc gacaacgtag gtatgggcaa
    23581 agaccacact ttgttcgcca aaattgacgg ttatgtcgaa ttcaaaacca aaggcgcgct
    23641 gaaccgtaaa actgtcagca tccgtcctta caccggttct gaagaataat tcaaccgata
    23701 cctgaagccg catcttttca cgatgcggct ttttacatac cccatacccg atttgataca
    23761 cattaagctg aaagtaaaaa tccgcataca ccctcccctg catatttctt caacaacggg
    23821 ttttgatata atcgcctatc tgttacagat agttcaaacg gcattcaaac ccttacaaat
    23881 gccgtctgaa atcatcggca tccgcctatg caaaggatat tttatgagtt tacacagcga
    23941 catcctcgtc gtcggcgcag gcccggcggg tttaagtttt gcagccgaac ttgccggcag
    24001 cggtttgaaa gttactctga tcgaacgcag cccactgact gttctgcaaa atcctcccta
    24061 tgacggacgc gaaatcgccc tgacccattt ttcccgcgaa atcatgcagc gtttgggtat
    24121 gtgggacaaa attcccgaaa acgaaatcta ccccttgcgc gatgccaaag tgctgaacgg
    24181 acgttccgac taccagctcc actttcccca accgactgaa gcgcgcggcg agcctgccga
    24241 ctgtttgggc tatctgattt ccaaccacaa tatccgccgc gccgcctatg aaatcgtatc
    24301 acaactcgac aatgtcagca ttctgaccga tacggccgtt aaagaagtca aaacatctga
    24361 taatgaagcg caagtctttt tggaaaatgg agacattcta accgcgcgtc tccttttggc
    24421 agcagacagc cgtttctcac aaacccgccg acaactcggt atttcttcag atatgcacga
    24481 ctacagccga accatgttcg tctgccgcat gaaacatacc ctttccaacc aacataccgc
    24541 ttacgaatgc ttccattacg gacgcaccat cgcactgctg ccactggaaa agcgcctgac
    24601 caataccgtc attacagtcg ataccgataa aatcaacagc gttcaaaacc tttcgcccga
    24661 agaattggcg gcaagcgtaa aagaacagct caagggcagg ctcggcgaca tggaactggt
    24721 cagcagcatc caccattacc cccttgtcgg catgattgcc aaacgtttct acggcaaacg
    24781 cagcgcgctg atcggcgatg cggctgtcgg tatgcacccc gtaacggcac acggcttcaa
    24841 cctcgggctt tccagcgcgg atattctggc aaaactgata ctcgaagccg aacagcgcgg
    24901 tcaagacatc ggcgcggctt ccttgttgga aaaatacagc agcaaacata tgctgcacgc
    24961 ccatccgctt tatcacggca ccaatatgat gctgaaactg tttaccaacg aaaccgcgcc
    25021 ggcaaaactg ttgcgcggtt tggtattacg agccggcaac aacttcccgc cgctgaaaaa
    25081 gctgattacc aaacaactga ccggttgaaa actgccgaaa tgccgtctga aacgtttcag
    25141 acggcatttt tgtattatga accgttttgc ggtttccata tccttggcag acggtttgca
    25201 gcagattcgc atacggcaga tgtttcaagc agaaaggaaa cacaataaaa ataaaaaagc
    25261 ctccgaacag tcggaggctt tgctttcaaa ctgaaattat ttcagtttgg tttctttgta
    25321 cactacgtgt ttgcgggcaa ccggatcgaa ttttttgatt tccagtttgc cgggcatagt
    25381 gcgtttattt ttggtagtgg tgtagaagtg gccagtacct gcaccggatt ccagtttgat
    25441 tttatcgcgc attgcagtaa tccttaatta aatagtgttt aaattaagct tcgccgcgag
    25501 cacgcaaatc agccaatacg acatcaatgc ctactttgtc gatggtacgc aatgcagcgt
    25561 tggaaacgcg caggcgaacc cagcggtttt cactttctac ccaaaaacga cgtgattgca
    25621 agttgggcaa aaaacggcgt ttggttttgt tgttggcgtg cgatacgttg ttgccggaca
    25681 tcgggcgttt accggtcact ttgcaaactc gtgccataat cagtctccaa acttctaaaa
    25741 tagtaaaacg cgatttatac cacgaaaaac aatgccgttt caagtgtttt aaagaaaaac
    25801 agatgttttg cgcgggtaag ccgcccgagt gccgcatctg cggaacgttt gcaggtgcga
    25861 aacagatggg gcgcgattat agcggatttc gggagcttct gcattcaaaa tgccgtccga
    25921 acatatttcc agtattttca gacggcattt caagttttca aatccctcaa tgtccaccgc
    25981 cgccgctgtg gaacggcggt ttcgccagcc agacaatggg aatcaggacg ataaacaaaa
    26041 tgctgccggc gaggaatatt tcgttcgatc cgatgatgaa gccctgttgc gtaatggtat
    26101 tgttgatgat gcctagagtt tgaccgtcgg aaatgccttg ttgggacaaa tgcgccgccg
    26161 tttcgtgcaa tgttgcggaa tagggcgtga tgtgttcggc aaaacgcgtg tggtgcaacg
    26221 cttcgcgccg ttcccacagg gtgctgacga cggatacgcc gacaccgccc atcagcacgc
    26281 gcaggaaatt cgacaggctg ccggcggcgg caatctgtcc gcccttcatg tgcgacagtg
    26341 tgatggtggt cagcggcagg aagaacatgg caacgcctac cccctgccaa aactgcggcc
    26401 agatgacgtt gccgatatcc atgtcggcat aaaaatcagt gcgccaataa aaagtgaagg
    26461 caaaggtcag gaagctggcg gttacgagca ggcgcatatc gattttattg ccgaacctgc
    26521 cgattaacgg agataggaag acggggagga tgccgacggg cgcggcagca agccctgccc
    26581 acgcggaggt atagcccaag ttggtctgca acactaacgg cagcagggtc agcgtcccca
    26641 tatacaccat gaaacccaac gaggtggcaa ttacgccgac ggtaaaattc cgatctttaa
    26701 acagcgataa atcgacaatc ggatattttt ctcccaattc ccaaacaata aaatacgaca
    26761 agcacaccag tgcgactacg cccaaggtaa tgatttctcc agaggcgaac cagtcaagtt
    26821 ccttacccct gtccagcatc atctgtaacg cgccgatacc gaccatcatc agcgtcagcc
    26881 cgacatagtc ggtcggcgtt ctgacggttg ccgtttcgcg gtgtttcaaa tgtttccatg
    26941 taatccatgc cgatatgatc ccgatgggga tattaatgaa gaaaatccaa ccccaatgcc
    27001 agtttccgga aatccaaccg ccgagtatcg gcccgaggac aggggcgaca acgacggtca
    27061 ttgcccacaa tgccagtgcc agcatccgtt ttgcgggcgg ataggatgcc attaacaggc
    27121 tttgcgacag gggaatcaac ggcccggcga taaagccctg caaaatgcgg aaaacaacca
    27181 gcgactgaag gttgggcgcg atgccgcaca gccacgatgc gatgacgaaa ccggcggcgg
    27241 cggcggtaaa caatttgacc tcgccgatac gttttgccaa aaagcccgtc agcggcacgg
    27301 aaacggcgtt tgccacggaa aaagaagtga tgacccacgt cccctgcgtg gttgccgcac
    27361 cgaggttgcc ggcaatgacg gggacggcga cgttggcgat ggtcgtatct aaaacctcca
    27421 taaatacggc aagccccaaa gacagcgtaa cccacgccaa tgccgcaccc ttaagcggtg
    27481 gataatccat tgtgtcctcc gtttcagacg gcatcagggc gcggattgcc cgaggatttc
    27541 atcgaccgtc cgatcgactt ccgaccagtc ggtactttcc atttccggca atgccgcacc
    27601 cggcgttttt gaaacaggcg cgcctgcggc ggaagtatcc actttaaccg tcatcgacaa
    27661 accgatacgc aacggatgcc tgtccacatc ttcgcggttc agcacgatac ggacggggac
    27721 gcgctgcacc actttaatcc agttgcccgt tgcgttttgc gccggaatca gcgaaaacgc
    27781 gctgcccgta cctgccgaaa accctgccac cctgccgcga taaacaattt gtttgccgta
    27841 caaatcggac accagctcgg caggctgtcc gattttcata tgccgcaact gcgtctcttt
    27901 aaaattggca tccacccaca catccgacag cggcaccacc gccatcagcg gcgcgcctgc
    27961 cgccacctgc tgcccgacct gcaccgaacg cttcgccacc tgaccgtccg ccggcgcgcg
    28021 gacttgcgtc cgccgaaggt tcagccacgc atctttcaac ctgccgattg ccgtctgaac
    28081 ctccggctgt tcgcgcaaag aaacatctcc gcccaaagcc gcgcgtgccg aagattcttc
    28141 cgccaaagcc gccttaaccg ccgcctgcgc ctgagacact gccgtacggg cgtgtgccag
    28201 ctcttcggcg gacaccgcac ccgattccgc caaagcggac cggcggcgca aatcatcctg
    28261 cgcgcgcgcc aaatccgcac ggcgcaaggc aacctgcgcc cccgcctgcg aagtggcggc
    28321 attttgccgg cggttttggc gcaccgcctg aaccaactcg ttttttgccc gctcgtaagc
    28381 cagcacatca ttatcgtcat ccaacaccgc cagcacgtcg ccttttttca cggcatccgt
    28441 atcgtcgtgc aaaaccttcc gcaccgtgcc gcccttttgc ggcgtaacct gaaccacgcg
    28501 tccggcaaca taagcgtctt ccgtttcctc ttcgtgctgc caccataaaa aaaacgccga
    28561 tccggcggct gcggcggaaa gcgcgaacag cagcgtcaat gccgtcaggc ggcgtttgcg
    28621 tttgacttgc gtgttttgaa gttttgtttc gtctgtgtgc gtttccataa gattgggttc
    28681 cgaatttcct gatttcatat aaacaatgtt ctttcaatag gaaatagatt ttatatccgc
    28741 gcaataattc aatcaagtca gtattttaga tgcaatattc aaaaaacaac gcttcaggcg
    28801 gcaaaactga aaatatattt cgtttatata tgacatggca aacaccgccg cccgcaccat
    28861 gccgtctgaa gaaaactaca caaataccgc cgcttatatt acaatcgccg ccccgtggtt
    28921 cgaaaacctc ccacactaaa aaactaagga aaccctatgt cccgcaacaa cgaagagctg
    28981 caaggcatct ctcttttggg taatcaaaaa acccaatatc cgaccggcta cgcgcctgaa
    29041 attctcgaag cattcgacaa caaacatccc gacaacgact atttcgtcaa attcgtctgc
    29101 cccgaattca ccagcctctg cccgatgacc gggcagcccg acttcgccac catcgtcatc
    29161 cgctatatcc cgcacatcaa aatggtggaa agcaaatccc tgaaactcta cctcttcagc
    29221 ttccgcaacc acggcgattt tcatgaagac tgcgtcaaca tcatcatgaa agacctcatc
    29281 gccctgatgg atccgaaata catcgaagtg ttcggcgagt tcacaccgcg cggcggcatc
    29341 gccgttcatc cgtttgccaa ctacggcaaa gcaggcacag agtttgaagc attggcacgc
    29401 aaacgcctgt tcgagcacga cgcacaataa accatccttc ccatatttca gacggcattc
    29461 cttcgatacg gtatgccgtc tgaacgcctt tccgaaaggt ttttatgtac gcattgaccg
    29521 ccgcacagca acagaaggca ctcttccggc tggtgctttt ccatatcctc atcatcgccg
    29581 ccagcaacta tctggtgcag ttccccttcc ggattttcgg catccacacc acttggggcg
    29641 cgttttcctt tcccttcatc ttcctcgcca ccgacctgac cgtccgcatt ttcggttcgc
    29701 acttggcgcg gcggattatc ttttgggtga tgttccccgc ccttttgctt tcctacgtct
    29761 tttccgtttt gttccacaac ggcagttgga cgggcttggg cgcgctgtcc caattcaaca
    29821 cctttgtcgg acgcatcgcg ctggcaagtt ttgccgccta cgcgctcgga caaatccttg
    29881 atattttcgt attcgacaaa ttacgccgtc tgaaagcgtg gtggattgcc ccggccgcat
    29941 caaccgtcat cggcaatgca ctggacacgt tagtattttt tgccgttgcc ttttacgcaa
    30001 gcagcgatga atttatggcg gcaaactggc agggcatcgc ttttgtcgat tacctgttca
    30061 aacttaccgt ctgcaccctc ttcttcctgc ccgcctacgg cgtgatactg aatctgctga
    30121 cgaaaaaact gacggccctg caaaccaaac aggcgcaaga ccgccccgtg ccctcgctgc
    30181 aaaatccgta aaacagctct gtagggaatc gggacggttg ggttttcttt tatttgcaaa
    30241 cactgccgga acggataaat ccggattccc acccacttcg tcattcccgc gaaagcggga
    30301 atccggtgcg ttggggttca gtcatttccg ataaactcct gccgcattgt atttctagat
    30361 tcccgctttc gcgggaatga cggcggatgg gtttccgtgc cgtggtgtcc ggattcccgc
    30421 cggtacggga atgacggcag cgcttccttg gcttagtttt attttaagcc actataaatg
    30481 ccgtctgaaa acctgtttgc agattttcag acggcattgt tttcgtcgtc ggttcaaact
    30541 tcaatccgat tgcgacacgg taaccggtat gccgcgcaag cgcgcaagca gcgcgtccgc
    30601 cttctgtttc tgcgcggcaa acgccgcctt gtccgcctgc gtcaattcgg gtgtcggcaa
    30661 tgcgaccgaa acaggattga cgggctgccc gttgatgcgc gcctcgtaat gcaggtgcgg
    30721 cccggtcgaa cgccctgtcg aaccgacaaa accgatgacc tcgccgccgc gcacattgcc
    30781 ttgtgcctgc gaaaacgcgc tcaagtgcgc gtacagcgtt tccacaccgt tggagtggcg
    30841 tatcatcacc gcgttgccgt atccgccctt ccggccttta aaggtaatca cgccgtcggc
    30901 ggaagccctg accggcgttc cctgcggtgc ggcataatcg atgcccgtgt gcagccgcca
    30961 tgtgtgcagg atggggtgca tacggtagcc gaacggcgaa gaaatgcgcg tatagaccag
    31021 cggctcgatg ttgaagccgc ctttttcctg caacaccctg ccgtcttcat cgtaataatt
    31081 gccgccccct ccgccttcct tgtccgaacg gtaatagaac gcctgatggg ttgtgtcgcc
    31141 cttgacaact tccgccgcca aaatatcgcc cgccgccacc tgctgcccgt ggaaatacag
    31201 gctgtcgtaa agcaggcgca cggcatcgcc ttccttcaaa ccgtcaaggc tgaagcggcc
    31261 ggcaaaaatc ccgcttaagg attcgcggat ttcgacgggc acttccgccc gcgccagcga
    31321 accgcgcgcc gacgttttga ccacgaccga acgcagtgtc ggcaaaacct tcatatccgc
    31381 atcagaagcc gaccgccgcc atatgccgcc ttttttttcc aaagcgacca gattgcgctc
    31441 gccgtcttcg tcggtaaaaa actgcacttc gcgcgcactg ccgtcgccgc cgaccaaaac
    31501 atgaaccgac tggtcggcac gcaaatgccg caaatcggct tcgccgccat atttttccgt
    31561 gattcgggca atctcgtccc gcgccatacc cgaacgcgcc agcacgtccg ccagcgagtc
    31621 gcccggctgc accgcctcct gcacccaata tgccgtctga acgccgctgc cgccccaaga
    31681 cagcggcggc agtttttgtt ccacgcgctg cggtctgacg cgctccgtcc cctctgtcga
    31741 agcaatgtat gccgccgaca ccagaataat cgaaacggca agcgcgcgca gcgcgtattt
    31801 ccgatgtttt gccgaaagtg ggaagacagc catattgttc ctttttgacg gcgtttgcgc
    31861 cgaatcctct ataatatccg cccgatcctc atttgtcagg catcaaacga tgaaactcac
    31921 cctcgccctc ccctcgctca atttggatga agacgaaatc cgaatacctc tatgcctgcc
    31981 cgccttcaac aaaatcctgc aatacggctc gccgcaccgc caaagctgca cggcttcggc
    32041 attttacgca cgttatttgt ggtgcggaag actggcacaa cgcccggcgc aaagcctgaa
    32101 tatgccgtct gaaaccgtcg cgctcgccac gcccgtctgg caaaaaatgg ggctgcatca
    32161 ggcaaatgtg ctgacggcgg aatatttgga tgtcggtacg gatgaagcgg aacggctctg
    32221 ccgcgatctg tcggcgtttt acggcgacat tccgtggcgt ttcgttcccg tcctgcccga
    32281 attatggctg gtttcccttc cccgcgcgta ccgctggggc gcgaagcccg ttttggactt
    32341 gggcggactg ttgggcgcgg acgatcagcc ggacggggaa gacgcgttgg aatggctgcg
    32401 cgtccaaacc gaaatccaaa tgtggctgaa cgctcacccc gtcaaccaca accgcaaaaa
    32461 acgcgggctg cccgaattga acggcttatg gctttgggac agtctgcacg gaagcgcaca
    32521 aggcggcacg cttttcgccg atacggtttg gagccgtttt cacccgaacc gccgagcatt
    32581 gcccgacagc ttccgagctt atgcggaaac ggcggcacac ctgcccgata cgcaccacat
    32641 cctgtttatg gacgatttgc gcctgaccgc cctgacaggg gacagggagc ggtatgcggc
    32701 aatattgcag cagtgggaag aacgctggtt cgcaccgctt tacgaagccg tccgcaccgg
    32761 caaaatcaaa cggctggaca tcgccacgga cggacaacac ggcggtacgc tgaccttcaa
    32821 acccacagac aggcggaagt tttggcgctg cacgaaaacc tttgacggaa tctggtaaac
    32881 ccgcccgccg caaccgcgac aatgccgtct gaagcccgaa tcgggtttca gacggcattt
    32941 taccccgcat cgccgttatg ccggaaaaac cggaatacgt ttccgcacac gcggcggcac
    33001 gcaaaacacc gcccgaaagc cgccgatgcc cgccgtgaca ccgccgccgg cagggtttcc
    33061 gccgaaaagg aacagcaatc agaacgtttt gttaaactcg acaaacgccc tgtttttctc
    33121 gtattcgtta aacacatcgt tgctccacgt ttcgcggtgc gacagcgtca ggcgcggcgt
    33181 gatgcctttg aaatgcaatg cccggtgcca aaggctcaag gatgtgtccg attctttatc
    33241 cctgcgcctt tcccctttaa aactgctgaa gaagccgggt ttttcataat gccgtttcgc
    33301 cacgccgagg cggaacagcg aagacaggcc gctgccgccc cattcctgcc cccaggcaaa
    33361 gcgcaggccg taacggttga aattgtcgcc acggtcggcg gggttgcgct cgcggtaaaa
    33421 atccaaaccg cccgtccaat attggcgcgc attccggtaa aacaccagcg aattggaaat
    33481 ttgcaaatgg gtattgtcgg aacgcgcccg gcgcgtattc ttcaaacgcc cccactccgc
    33541 cgaagacagc gtttgccatc tcggggtttg ccaacggttg aaataaaggc gtgcgccgtt
    33601 ggcgtaagaa taagcgtcgt tgccgtaggt gcggcgttcg tggaacactg ccagcccgac
    33661 atctttacgc cggtcggcaa aaccgatgcc gccggaaaca cctgccgtca tatcgttgaa
    33721 tttcttattc cccggataaa ccctgccgga cacgtcgccg cccgccgtcg tgtaccagcc
    33781 gtttttcagc gaccattttt tctccgcgcc gaaccggtaa ttgaccgccg tgccgtccac
    33841 ctgtttcggg aaagtccaat tgccgtactg ctgctgtttc ggggcttggt tgatattgtg
    33901 ttcgcgggta acgctgaaac cgccgtttac cttccacgca tcgcgttcgc gcaatgcctt
    33961 gcggtacagc tcgacctgct ccataagctg cggcggcaga tcttctgttt tcaggcggtc
    34021 gaactggtct gccgccgcct cgttctgcct gtcttcaaac aatgccgccg ccaaacgcat
    34081 acggacggcg ggcgcgtcgg gttgggcggc aatcaattcc cggtaatggg aaaccgcctc
    34141 cttcaccctg ccctctgcct gcgccaaaat cccttgtgca taaagtgcca acatcttatc
    34201 ctgccgcgcc tgttgtaggt aaatcggcaa aatcacgcgg ataccggcaa tattgtttga
    34261 gaccactgcg gaatacatgg cacgcgacaa caattcggga tttttcagca gggtttcgcc
    34321 gtcaacctgc aaaacttttc ctttttcccg cacctgcccc ggtaccttct ccctgtcgat
    34381 cggtttgacc tccgcttcat gaagcctgaa ctcgggacgg cttctcaaat ccggttcgcg
    34441 cggtgtttct tcggcgtatg ccgcgcttgc caataaaggc agcagcatcc atctgtttcg
    34501 tgcaggcata aatgtcttcc cacaaaaata aaaataaaaa ataaccatta tcaaccggcg
    34561 gattgtatca gttttttatg ccgctgttcc aaaccctgcc gcccgatgcc gcgacaatgc
    34621 cgtctgaagc cagaatcggg tttcagacgg cggcaaaaaa ccggcggttt tccgccggtt
    34681 tcatcattat gccccgccct tctgggcggc agggttcgga tcggtttgcg gggcggcgca
    34741 cggggccgtg caggtgtgcc gcctgtgtgt caaaacattt cccggcgcat caaaacaacc
    34801 atttgaccga caatgcggct tctttgtcgc cgtccgtcct tttgccgtat ccgatgcgtg
    34861 cggacatatg gcctttccaa ccggcttcaa tgccaaaccg cccttcgagc gccgtcctgc
    34921 ctgccagcgt ctgtttttcg ccgtccattt ccatgccgaa agattttgac ctgtgcaaaa
    34981 cattaaaagc ggcaaaaggc tgaagattga caccgttacg caaagcaaaa cgggtttttg
    35041 cccgaatgcc ggcgcggatt tgccactgac cgctgccgag cagcccgacc gccgcccctc
    35101 gctgtcggta aagccgccgt ttacgcccaa gtaggtaaac tgcgcctgcg gttgcaggta
    35161 aaactgcata ttactgcctt atatattgcg tccctaagaa ggggcgatta acaaaaatta
    35221 acgccccttc ttagggacgc aatatataag gagtgagtac ctgttcttct attaaagaat
    35281 taatcttttg aggcattttc tgttgaagtt tattaaacac atcttcagaa attccaaact
    35341 gctttattat ctcttcccta acccgttttt tattttcttc ccaagcctcg ggtcttgttt
    35401 tgtataaatc ctgtaattgc tttttaactg cctgctttaa atcagttcgg taattatctg
    35461 taatattgat ttcgtcatac aaggcttcag atgatttttg atcggtttta ccgctggaga
    35521 aataatggaa aacaccgttt gccggcttgt ctgaaaatga aaaatcggca gtatcgccaa
    35581 catttgtagg acgagtatcc gaaccgggct gataactgaa agcaccgaag caatcataaa
    35641 gatatgctgc acgggccgcg ccaaacgtgg gaatttctgt cttccgccgc ttctaaaata
    35701 aatttgtttt taatctcggc ttctttgacg ggatcgtacg catccgaacc ccaagcatat
    35761 ggcgtgcaaa acagcgataa aacagataat gttaatagat taacccgacc gcttgctttc
    35821 agtgtcatga gaaattttcc tttgtcaagt gttaaagtta taatgattat atactatatc
    35881 acactacatc gcaatgaaaa atcgggaaaa acaaaaaacc cctccgccgt cattcccgcg
    35941 aaagcgggaa tccagaattt caacgcctca agaatttatc ggggatggcc ggggtttaaa
    36001 ggtctgggtt tccgcctgcg cgggaatgac ggtttagaaa tcacccgaaa cgagaaaaag
    36061 aaccgaaacc ggacaagtcg gattcccgcg cgggcgggaa tgacgataag cgtaatttca
    36121 atccgccata ttgtcttttc acttgcctga aaacagattt cagccgccta tccctgcatc
    36181 cggcggctga tgtagtcggc aatcaaatcc cgcttttccc aaaaaaactt ctgcattttt
    36241 tgttcggaat acagtttgcg gaccagagtg ggcagaccgc tgtggacgat ggcggcgatt
    36301 tctttgttgc cttcttcttc ctgcattgcc gtctgaaaat ccaaacccct gcctttcaga
    36361 tatgctgcaa ggcggttgcg gatgtgtgaa ttgagttcga tggatttgag cattttgcct
    36421 ccgttttgcg gtaaggattt attcgatttg tttatccgcc tgattttatc acgtttccca
    36481 agcggtttgc ctgtacaata ccgcttttcg gaagggtgtg ggcgatgatg ttttcttggt
    36541 tcaagctgtt tcacttgttt tttgtcattt cgtggtttgc agggctgttt tacctgccga
    36601 ggattttcgt caatatggcg atgattgatg cgccgcgcgg caatcccgag tatgtgcgcc
    36661 tgtcggggat ggcggtgcgg ttgtaccgtt ttatgtcgcc tttgggtttc ggcgcggtcg
    36721 tgttcggcgc ggcgataccg tttgccgccg gccggtgggg cagcggctgg gttcacgtca
    36781 aactgtgttt gggcttgatg ctcttggctt atcagttgta ttgcggcgtg ctgctgcgcc
    36841 gttttcagga ttacagcaat gctttttcac accgctggta ccgcgtgttc aacgaaatcc
    36901 ccgtgctgct gatggttgcc gcgctgtatc tggtcgtgtt caaaccgttt tgacggagca
    36961 tcttatgccg atagaaatcg aacgccgttt tttgattgaa aacgacaaat ggcggcaata
    37021 cgccgatgag ccgctgctgc tgaaacaggg ctacctgtct gttgaaaaag aacgcaccat
    37081 ccgcatccgc attgccggaa aacgggcgtg gctgacgctg aaaggctata tttcggaaat
    37141 cagccgcagc gagtgcgaat acgaaatccc gcttgccgat gcggaaaaga tgatggaaac
    37201 gatgtgcccg tttaagatgg aaaaacggcg ttatccggtc agatggggcg gcagcctgtt
    37261 tgaagtcgat gttttccttg gcgaaaactc gcctttggtc gtcgcggaaa tcgagttgcc
    37321 cgccgaaaat gccgattttg accgtccgga ctggctgggg cgcgaaatca cttcagacgg
    37381 tatgtttacc aatgcctatt tgagcaaaca tccgttctca agctggaaaa atgccgtctg
    37441 aagcggcttc agacggcatc tgccttgcgg ttatcccttg aggtaatgca cgaatgtata
    37501 tgcaacgcct ttgctgctga cacggcgttc cgtccgctct gcttctttcc aatgcgtccg
    37561 gtctatttct gggaaaaatg catctccttc cacagacaaa tccacttcgg ttatccgcaa
    37621 atcggtcgct aatggcatcg cttgtccgta tatctgcgcg ccgcccataa tgacggcttc
    37681 ttccgcgccg gcgcacaatg ccaatgccgc ctccaaactt gccaccgttt ccgcgcctgc
    37741 cgcgcaataa tccgcctgcc ggctgatgac gatgttcctc cgtccgggca ggggtttgac
    37801 gggcagggat tcccacgttt tccgccccat aatgacgggt ttgcccaagg tataggcttt
    37861 gaaaaatgcg aaatcttcgg ggatgtgcca aggcatagca ttgcccgccc cgatgcacag
    37921 gttttccgca cacgccgcaa ttatggttat tttgagcata ttttctccac aaaaatgccg
    37981 tctgaacgct tcagacggca ttttatgaca ttctgcgtca aaaatctcaa cctgcgcgcg
    38041 ccatacgttt tgtgttcgca cccgccaaca atgccaacaa ttcttcagtc gcgccccaac
    38101 cgatacacgc atcggtaatg ctcttgccgt acacttccgg cttgtcctgc ctgccttcga
    38161 ccaaatggct ttccaccatc acgcccatga tattgccgcc gtcctgttcc aattggtcgg
    38221 caatgtcttg tgccacttcc atctgccggg tgtaatcctt gcggctgttg gcgtggctgc
    38281 aatcgatcat cagcttgtcg gttacccctg ccgcacgcag ttgttccacc gcctcgctga
    38341 cgtgtcccgc atcataattc ggctcttttc cgccgcgcaa aatgacatga cagtcgggat
    38401 tgccgccggt atggacaatg gcggaatgcc cggccttggt tacagacaag aaatgatgcg
    38461 aatggctcgc cgcaccgatt gcgtcgatcg caatcttcaa attgccgtcc gtaccgtttt
    38521 taaagccgac ggggcaggac agaccgcttg ccaattcgcg gtggacttgg ctttcggtcg
    38581 tccgcgcgcc gattgccccc caagaaatca ggtcggcata atactggggt gtaatcatat
    38641 ccaaaaattc ggtagaggca ggcatgccca tattattcag ggacaacaac aggctgcgcg
    38701 cctgacgcaa accgaaattg atgtcgaacg taccgtccaa atgcgggtcg ttaatcaaac
    38761 ctttccaacc caccgtcgtt ctcggcttct cgaaataaac gcgcatcacg atcaaaagct
    38821 cgttttcata ccgcttgcgg agtttcaaca gacgttccgc atattccaac gccgctttcg
    38881 gatcgtgaat cgaacacggc ccgataatga ccaacagccg cctgtccctg ccgtgaacca
    38941 aatcggaaat ttcctgacgg gtgcggtgaa ccaagcccga agcctctttg gaaatcggca
    39001 gctcgtaaag atgggcaatc ggcggcaaca actctttaac ttctttaatc ttaatatcgt
    39061 ctgtggggta atggtgtgtc atcttgcatc tttcctgatg ggtttttata cgcggcttag
    39121 gttacatcgt ttaccttgca caaacaagtg caacatatta ctacaaagct attttatttc
    39181 aaaataactg atatatcttt tttcatagat tttaaatttc atatttttaa tattttagga
    39241 attttatttc aaagaaaaag aaacagattg ataaaaacaa tacccgccat tcattcgggc
    39301 aaaaccgcac ggaacatccg ttttcccgtt tccacgacaa tccggtacag gacaccccgc
    39361 tgccgattcc gcctgcacat ccggcaaaat ttcatacgca aggaaaatgt tgaaaacaaa
    39421 aagaactaat tgcttgaaaa aagatttaat tggaatgcgg gtatttccca cactttctcc
    39481 atttatttat cctcattata aaaacatgaa aaaaaacccc tgcttgcact ctcacttgca
    39541 gccgtgtccg ccgttacggt agccgatatt cccgagtcta acggcaaagg cgaatacaaa
    39601 gtctttaccg cttatgttaa aggcaaagcc actgccggca atttgaaaga aaaataacgc
    39661 acaccgtcca tcggttttcg ttcaacgaac gccacgatat gcgttttaag gcagagtacg
    39721 cccgaagcaa aaacgatgca gacgacaaaa gagggaatgc acacatcgct aacggcgcaa
    39781 taggcaaaat ggctgccgtg tatgccggtt atacctacac ccaaccgggc ggcgaatcca
    39841 cacgtttccg tggcggtttg gagcgtgtcc aagttaaacg ttatctggga gaaaaatgct
    39901 gcggctacgc ctgtccaaaa agcgaacgct tcaccctgca agcagatggt gaaatccgtc
    39961 aaaaccttgg cgaaggctgg tttctgaagc tatgagcggg gtggctgtct atctccgcaa
    40021 caaaaacaaa atagcaattg cctcccaaga tgccaaccta aactccaaag gccggttcgt
    40081 cagcagcggt ctgaatgtgg gcaaacaact gaccggcagt ctcggcgtag agtttgaccc
    40141 atactaccgc caccgcgcaa tccgcaagtc tgccgaattt gtcccgaaca ctaccaaaac
    40201 caaaacagac agcgaaaagt ttaacgagta gggcttccgc gtagccgcaa cgttctaatt
    40261 cccttacgta taaaaagcag cataaaaatg ctgccttttc ccatttccaa gggaagtatt
    40321 ccgtagtgca gcttacctca ttgtttcaca caaaccgaaa atgccgtctt aaaaccaatt
    40381 ttcagacggc atttgtccct taaaacacgt tttttcaagc gcgaccatac caaacaccca
    40441 caggttgcct tccacccttc gtacaacctt tgctccatca aacatccact tcctaaatac
    40501 tgatgtatac tg
//
LOCUS       DAWTQS010000010        40268 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.010, whole genome shotgun sequence.
ACCESSION   DAWTQS010000010 DAWTQS010000000
VERSION     DAWTQS010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 40268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40268
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.010"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            338..2393
                     /locus_tag="WNA25_000554"
                     /pseudo
     CDS             338..2393
                     /locus_tag="WNA25_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219143.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
     gene            2518..4068
                     /locus_tag="WNA25_000555"
     CDS             2518..4068
                     /locus_tag="WNA25_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFA9823378.1"
                     /translation="MKKPLISVAAVLLGVALGTPYYLGVKAEESLTQQQKILQKTGFL
                     TVESHQYDRGWFTSTETTVIRLKPELLHNAQKYLPDNLKIVLEQPVTLVNHITHGPFA
                     GGFGTQAHIETEFKYAPETEKVLERFFGKQVPVSLANTVYFNGSGKMEVSVPAFDYEE
                     LSGIRLHWEGLTGETVYQKGFKSYRNSYDAPLFKIKLADKGDAAFEKAHFDSETSDGI
                     NPLALGSSNLTLEKFSLEWKEGVDYNVKLNELVNLVTDLQIGAFINPNGSIAPSKIEV
                     GKLAFSTKTGESGAFIDSEGRFRFDTLVYGDEKYGPLDIHIAAEHLDASALTVLKRKF
                     AQISAKKMTEEQIRNDLIAAVKGDASGLFTHDPVLNIKIFRFTLPQGKIDVGGKIMFK
                     GMKKEDLNQLGLMLKKTEANIRMSIPQKMLEDLAVSQAGNIFSVNAEDEAEARASIAD
                     INETLRLMVDSTVQSMAREKYLTLDGNQIDTVISLKNNALKLNGKTLQNEPDPDFDEG
                     DMVSGQPH"
     gene            complement(4492..6276)
                     /gene="lpdA"
                     /locus_tag="WNA25_000556"
     CDS             complement(4492..6276)
                     /gene="lpdA"
                     /locus_tag="WNA25_000556"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFA9823379.1"
                     /translation="MALVELKVPDIGGHENVDIIAVEVNVGDTIAVDDTLITLETDKA
                     TMDVPAEVAGVIKEVKVKVGDKISEGGLIVVVEAEGAAAAPKAEAAAAPAQEAPKAAA
                     PAPQAAQFGSAADAEYDVVVLGGGPGGYSAAFAAADEGLKVAIVERYKTLGGVCLNVG
                     CIPSKALLHNAAVIDEVRHLAANGIKYPEPELDIDMLRAYKDGVVSRLTGGLAGMAKS
                     RKVDVIQGDGQFLDPHHLEVSLTAGDAYEQAAPTGEKKIVAFKNCIIAAGSRVTKLPF
                     IPEDPRIIDSSGALALKEVPGKLLIIGGGIIGLEMGTVYSTLGSRLDVVEMMDGLMQG
                     ADRDLVKVWQKQNEYRFDNIMVNTKTVAVEPKEDGVYVTFEGANAPKEPQRYDAVLVA
                     AGRAPNGKLISAEKAGVAVTDRGFIEVDKQMRTNVPHIYAIGDIVGQPMLAHKAVHEG
                     HVAAENCAGHKAYFDARVIPGVAYTSPEVAWVGETELSAKASGRKITKANFPWAASGR
                     AIANGCDNGFTKLIFDAETGRIIGGGIVGPNGGDMIGEVCLAIEMGCDAADIGKTIHP
                     HPTLGESIGMAAEVALGVCTDLPPQKKK"
     gene            complement(6294..6728)
                     /locus_tag="WNA25_000557"
     CDS             complement(6294..6728)
                     /locus_tag="WNA25_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823380.1"
                     /translation="MSYLYRGISCQQDEQNNGQLKPKGNKAEVAIRYDGKFKYDGKAT
                     HGPSVKNAVYAHQIETDLYDGCYISTTTDKEIAKKFATSSGIENGYIYVLNRDLFGQY
                     SIFEYEVEHPENPDEKEVTIRAEDCGCIPEEVIIAKELIEIN"
     gene            complement(6829..8391)
                     /gene="aceF"
                     /locus_tag="WNA25_000558"
     CDS             complement(6829..8391)
                     /gene="aceF"
                     /locus_tag="WNA25_000558"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFA9823381.1"
                     /translation="MSIVEIKVPDIGGHESVDIIAVEVKAGDTIAVDDTLITLETDKA
                     TMDVPADAAGVVKEVKVKVGDKISEGGVILTVETGAAAAEAAPAAAAEAQPAPAAAGG
                     ATVQVAVPDIGGHTDVDVIAVEIKVGDTVAEDDTLITLETDKATMDVPCTAAGVVKAV
                     FLKVGDKVSEGSAIIEVETAGSAAAAPAPAAQAAAPAAVPTSASPAAAKIDEAAFAKA
                     HAGPSARKLARELGVDLGQVKGSGLKGRIMGDDIKAFVKSVMQGGAAKPAAAGASLGG
                     GLDLLPWPKVDFSKFGNVEVKELSRIKKISGQNLSRNWVVIPHVTVHEEADMTELEEF
                     RKQLNKEWEREGVKLSPLAFIIKASVSALKAFPEFNASLDGDNLVLKNYFNIGFAADT
                     PNGLVVPVIKDVDQKGLKQISQELTELSKKAREGKLKPQEMQGACFTISSLGGIGGTG
                     FTPIVNAPEVAILGVCKSQIKPVWNGKEFAPRLMCPLSLSFDHRVIDGAAGMRFTVFL
                     ANLLKDFRRITL"
     gene            complement(8541..11204)
                     /gene="aceE"
                     /locus_tag="WNA25_000559"
     CDS             complement(8541..11204)
                     /gene="aceE"
                     /locus_tag="WNA25_000559"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFA9823382.1"
                     /translation="MSTQLHDVDPIETQEWLDALSSVLEYEGGERAQYLLENLLKYCR
                     DKGVRMPHGTTTPYLNTVSVENEKGIPGDQNIEHRIRAFVRWNAAAIVLRAGKKDLEL
                     GGHIASFQSAATMYEVGFNHFWKAKGEGEEGDLVFFQGHVAPGIYARAFVEGRLTEDQ
                     LNNFRQEVDGHGLPSYPHPHLLPDFWQFPTVSMGLGPIMAIYQARFLKYLESRGLAKT
                     KGRKVWCFCGDGEMDEPESQGAIALAAREGLDNLIFVINCNLQRLDGPVRGNGKIIQE
                     LEGNFAGAGWNVVKVIWGRRWDRLLAKDKDGILRQRMEECLDGDYQTYKSKDGAYVRE
                     HFFNTPELKALVADMTDEQLWALNRGGHDPQKVYNAYDRAANHADGKPTVILAKTIKG
                     YGMGASGEGQNVAHQAKKMDKASLKQFRDRFDIPVTDEQIESGDLPYLTFAPDTEEYK
                     YLHARRDALGGYLPQRKPTQEVLEVPELSAFDAQLKSSGEREFSTTMAFVRILSTLLK
                     DKKIGKRVVPIVPDESRTFGMEGMFRQYGIWNPKGQQYTPQDKDQLMFYKESVDGQIL
                     QEGINEPGAMADWIAAATSYANSDFAMIPFYIYYSMFGFQRIGDLAWAAGDMHARGFL
                     LGGTAGRTTLNGEGLQHEDGHSHIQADLIPNCVSYDPTFQYEVAVIVQDGLRRMYANN
                     EDVFYYITLMNENYTHPDMPEGAEQDILKGMYLLKAGGKGDKKVQLMGSGTILQEVIA
                     GAELLKADFGVEADIWSCPSFNLLHRDAIETERFNRLHPLEAEKVPFVTSQLQGHDGP
                     VIAATDYIRSYADRIRAYIPNDYHVLGTDGFGRSDSRANLRRFFEVDRYNVAVAALAA
                     LAEQGKVSKETVQQAIEKYGIKADSAPSWKR"
     gene            11903..13615
                     /locus_tag="WNA25_000560"
     CDS             11903..13615
                     /locus_tag="WNA25_000560"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFA9823383.1"
                     /translation="MKASQFFISTLKEAPAEAAFASHKLMIRAGLIKANASGLYTWMP
                     MGLRVLRKVENVVREEMARAGSVELLMPVVQPAELWQESGRWEFYGKELLRLKDRHER
                     DFCMGPTCEEVIADIVRKEINSYKQLPKNFYHIQTKFRDEVRPRFGVMRAREFVMKDA
                     YSFHADYASLQATYDAMYDAYCRIFTRLGLAFRPVAADTGSIGGTGSHEFQVLAESGE
                     DVIAYSDTSDYAANIELAPTLPLKGERAAAQAVLTKVHTPNVKTIESLVEFLNIPVEQ
                     TLKSIVVEGENEGELVLLLLRGDHEFNDIKAEKLAGVKSPLTMASPAAIVEQFGANGG
                     SLGPVGFTGKVYADFATEKGADWVIGANEDDYHYTGFNFGRDAAEPEFVDLRNVVEGD
                     ESPDGQGRLKLARGIEVGHVFQLRGKYTQAMNVSFLDNNGKSQIMEMGCYGIGITRVV
                     AAAIEQNNDEKGIIWTKAMAPFEVVIVPMNYKKSDTVREAADRIYAELLAAGADVLLD
                     DRDERAGVLLNDSELLGIPHRIVIGDRALKEGNVEYAERRDNEAQAVAIGEIVARVTA
                     SLNA"
     gene            13678..14334
                     /locus_tag="WNA25_000561"
     CDS             13678..14334
                     /locus_tag="WNA25_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFA9823384.1"
                     /translation="MASVFLEGEAVEVGNIFCIGRNYAAHVEELKNEIPSEPVVFMKP
                     SGSILNSGGTILLPEFSRDVQFECELVLLIGRDSDGTGEGEDILGCVAGYGVGLDLTA
                     RDIQCRLKAQGLPWLKAKGFRHSACVSDFAAADRIGNQDKVLFSLKQNGVLKQRGETG
                     LMIYPIREILHKLAADYGLGKGDLVFTGTPSGVGAIGAGDNLALELDGLVCASFTIGC
                     "
     gene            complement(14344..14799)
                     /gene="ruvX"
                     /locus_tag="WNA25_000562"
     CDS             complement(14344..14799)
                     /gene="ruvX"
                     /locus_tag="WNA25_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFA9823385.1"
                     /translation="MHKIPKGTALAFDFGEARIGVAQGDAELGLSHPLATVTGGSNDE
                     KFAAIAKLVQEWQPRYFVVGLPVHADGTEHEMTHLSRKFGRRLNGRFNLPVYWVDERL
                     SSVYAESLLSEAQVLGKKRKSVLDQVAAQAILHGFLEGGPAECFNGREG"
     gene            complement(14792..15340)
                     /locus_tag="WNA25_000563"
     CDS             complement(14792..15340)
                     /locus_tag="WNA25_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFA9823386.1"
                     /translation="MNLSNHFLVAMPDMEDAFFSQSVVYICKHDEDGALGIAINKPSP
                     ITMDMIFSATGKNIPMRMQHDSVMMGGPVQVERGYVVHTPIGNWQSSIGVSDGIALTS
                     SRDVLENISREGAVDKALISIGYSSWSKGQLERELADNAWLTVPADEHILFDIPYEHR
                     YAAAFAKLGIDPLALFSGAGHA"
     gene            complement(15342..15902)
                     /locus_tag="WNA25_000564"
     CDS             complement(15342..15902)
                     /locus_tag="WNA25_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HFA9823387.1"
                     /translation="MNRLLLLSAAVLPTACGSGETDKIGRASTVFNMLGKNDRIEVEG
                     FDDPDVQGVACYISYAKKGGLKEMVNLEEDASDASVSCVQTASSISFDETAVRKPKEV
                     FKRGTGFAFKSRQIVRYYDPKRKAFAYLVYSDKIVQGSPKNSLSAVSCFGSGIPQTDG
                     VQADTSGKLLAGACIISNPIKNPDKR"
     gene            16229..18025
                     /locus_tag="WNA25_000565"
     CDS             16229..18025
                     /locus_tag="WNA25_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HFA9823388.1"
                     /translation="MQYKPLLLALMLVFSAPAVAAHDAAHNRSAEVKKQAKNKKEQPE
                     AAEGKKEKGKNAAVKDKKTGGKEAAKEFKKTAKNRKEAEKEATSRQSARKGREGDKES
                     KAEHKKAHGKPVSGSKEKNAKTQPENKQGKKGAKGQGNPRKGGKAEKDTVSANKKARS
                     DKNGKAVKQDKKYREEKNAKTDSDELKAAVAAATNDVENKKALLKQSEGMLLHVSNSL
                     KQLQEERIRQERIRQERIRQARGNLASVNRKQREAWDKFQKLNTELNRLKTEIAATKA
                     QISRFVSGNYKNSQPNAVALFLKNAEPGQKNRFLRYTRYVNASNREVVKDLEKQQKAL
                     AVQEQKINNELARLKKIQANVQSLLKKQGVTDAAEQTESRRQNAKISKDARKLLEQKG
                     NEQQLNKLLSNLEKKKAEHRIQDAEAKRKLAEARLAAAEKARKEAAQQKAEARRAEMS
                     NLTAEDRNIQAPSVMGIGSADGFSRMQGRLKKPVDGVPTGLFGQNRSGGDVWKGVFYS
                     TAPATVESIAPGTVSYADELDGYGKVVVIDHGENYISIYAGLSEISAGKGYTVAAGSK
                     IGTSGSLPDGEEGLYLQIRYQGQVLNPSGWIR"
     gene            18152..19627
                     /locus_tag="WNA25_000566"
     CDS             18152..19627
                     /locus_tag="WNA25_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HFA9823389.1"
                     /translation="MSKPVFKKIALYTLGAISGVAVSLAVQGFAAEKDGRDNEVLPVQ
                     SIRTMAEVYGQIKANYYHDKPDADLFEGAMKGMVAGLDPHSEYMDKKGYAEIKESTSG
                     EFGGLGMEIGQEDGFVKVVSPIEDTPAERAEVKSGDFIVKIDNVSTRGMTVSEAVKKM
                     RGKPGTKITLTLSRKNADKPIVVNLTRAIIKVKSVRHHLIEPDYGYIRVSQFQERTVE
                     SVNTAAKELVKENKGKPLKGLVLDLRDDPGGLLTGAVGVSAAFLPSEAVVVSTKGRDG
                     KDGMVLKAVPEDYVYGMGGDPLAGIPAELKTIPMAVLVNSGSASASEIVAGALQDHKR
                     AVIVGTQSFGKGSVQTLIPLSNGSAVKLTTALYYTPNDRSIQAQGIVPDVEVKDKERT
                     FESREADLVGHIGNPLGGEDVNSETLAVPLEKDADKPAAKEKGKKKKDEDLSSRRIPN
                     PAKDDQLRKALDLVKSPEQWQKSLGLAAKKPVSNKDKKDKK"
     gene            19735..19836
                     /locus_tag="WNA25_000567"
                     /pseudo
     CDS             19735..19836
                     /locus_tag="WNA25_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            19957..21984
                     /gene="uvrB"
                     /locus_tag="WNA25_000568"
     CDS             19957..21984
                     /gene="uvrB"
                     /locus_tag="WNA25_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HFA9823390.1"
                     /translation="MEVIRYPNSPFKLHQPFPPAGDQPTAIAGLLEGLSDGLAYQTLL
                     GVTGSGKTYTMANVIAQSGRPAIIMAHNKTLAAQLYAEMREFFPENAVEYFVSYYDYY
                     QPEAYVPSRDLFIEKDSAINEHIEQMRLSATKNLMTRDDVIIVATVSAIYGIGDPTEY
                     QQMVLSVKEGDTIEQRDIIATLVSMQYERGDLDFKRGSFRVRGDVIDVYPAESSENAL
                     RISLFDDEIDRLDMFDPLSGSLHQRVGRYTVFPSSHYVTPRDTVLRACESIKEELRER
                     IEFFAREQRPVEQQRIEQRTRFDLEMLYEMGFCKGIENYSRHFSGKKEGEPPPTLMDY
                     LPDNAIMFIDESHVTVTQIGGMYKGDALRKQNLVDYGFRLPSARDNRPLKFHEFEKVM
                     PQTVFVSATPAKYEEEHAGQVVEQVVRPTGLVDPQIIIRPVATQVDDLMSEINDRIQK
                     GERVLVTTLTKRMAEQLTDYYSELGIKVRYLHSDIDTVERVEIIRDLRLGLFDVLVGI
                     NLLREGLDIPEVSLVAILDADKEGFLRSHRSLIQTIGRAARNVNGVAILYADKITDSM
                     KAAVDETERRREKQIKFNEEHGIVPQQIKKQVKDIIDGVYHEEDSGKGRRQGKNKVKV
                     GEIHNEEDAIKEIAKLEKAMQQAARDLQFEEAAVLRDRIRNIKENLLFGAE"
     gene            complement(22162..22920)
                     /locus_tag="WNA25_000569"
     CDS             complement(22162..22920)
                     /locus_tag="WNA25_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFA9823391.1"
                     /translation="MPRFPRTLPRLTAVLLLACTAFSAAAHGNHTHWGYTGHDSPESW
                     GNLSEEFRLCSTGKNQSPVNITETVSGKLPAIKVNYKPSMVDVENNGHTIQVNYPEGG
                     NTLTVNGRTYTLKQFHFHVPSENQIKGRTFPMEAHFVHLDENKQPLVLAVLYEAGKTN
                     GRLSSIWNVMPMTAGKVKLNQPFDASTLLPKRLKYYRFAGSLTTPPCTEGVSWLVLKT
                     YDHIDQAQAEKFTRAVGSENNRPVQPLNARVVIE"
     gene            complement(23329..23676)
                     /locus_tag="WNA25_000570"
     CDS             complement(23329..23676)
                     /locus_tag="WNA25_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823392.1"
                     /translation="MTYLKVIAISIVLYILLLQINLKMLEKRIDFLVENIDKYYQQYG
                     SYPNNFDFISTKTDFTTESYCDFWDKNIAGYGNCYFVKNDKDYTILVMGFSSKILFSS
                     HNKIKEFNSNKYD"
     gene            complement(24000..24716)
                     /gene="trmB"
                     /locus_tag="WNA25_000571"
     CDS             complement(24000..24716)
                     /gene="trmB"
                     /locus_tag="WNA25_000571"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFA9823393.1"
                     /translation="MTDTAENQTPNDRQAGHPRSIRSFVLRQSHMTAAQQRAIDTLWD
                     SFGIDYQATPADLDARFGSSRPKILEIGFGMGMASAEIARRLPETDFLAIDVHGPGVG
                     NLLKLINENHLENIRVMRHDAVEVVKNMLQDGSLDGIHIFFPDPWHKKRHHKRRLIQA
                     PFIAKLLPKLKTGGYIHLATDWEEYAQQMLEVLSSFDSLQNTAADYAPTPDYRPETKF
                     EARGKRLGHGVWDLVFKRIG"
     gene            complement(24838..26242)
                     /locus_tag="WNA25_000572"
                     /pseudo
     CDS             complement(24838..26242)
                     /locus_tag="WNA25_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674669.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
     gene            complement(26359..28245)
                     /gene="uvrC"
                     /locus_tag="WNA25_000573"
     CDS             complement(26359..28245)
                     /gene="uvrC"
                     /locus_tag="WNA25_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFA9823394.1"
                     /translation="MPSEAVFQMIDMNTENRSPEQFDIPLFLKNLPKLPGVYRFFDEG
                     GNVLYVGKAVNLKRRVSGYFQKNDHSPRIALMVKQVRHIETTITRSEAEALILENNFI
                     KALSPKYNILFCDDKSYPYLMLSGHQYPQMAYYRGTLKNPNQYFGPYPNSNAVRDSIQ
                     VLQKVFMLRTCEDSVFEHRDRPCLLYQIKRCTAPCVGHISEEDYCDSVRQAATFLNGK
                     TDELTRTLQHKMQTAAANLQFEEAARYRDQIQALGIIQSNQFIDSKNPNNPNDIDLLA
                     LAVSDGLVCVHWVSIRGGRHVGDKSFFPDTKNDPEPNGQDYAEAFVAQHYLGKSKPDI
                     IISNFPVPDALKEALEGEHGKQMQFVTKTIGERKVWLKMAEQNAQMAITQRHLQQSNQ
                     QHRIDELAKILGMNSDGINRLECFDISHTQGEATIASCVVYDEQNIQPSQYRRYNITT
                     AKPGDDYAAMREVLTRRYGKIQEAEANGESVKWPDVVLIDGGKGQIGIAVSVWEELGL
                     HIPLVGIAKGPERKAGMEELILPFTGELFRLPPNSPALHLLQTVRDESHRFAITGHRK
                     KRDKARVTSSLGDIPGVGSKRRQALLTRFGGLRGVIAASREDLEKVEGISKALAETIY
                     NHLH"
     gene            complement(28300..30477)
                     /locus_tag="WNA25_000574"
     CDS             complement(28300..30477)
                     /locus_tag="WNA25_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HFA9823395.1"
                     /translation="MQQKIRFQIEGMTCQACASRIEKVLNKKDFVESAGVNFASEEAQ
                     VTFDGSKTSVADIAKIIEKTGYGAKEKTEDTLPQPEAEHHIGWRLWLLLTINIPFLIG
                     MVGMMLKGLNWTRHDWMIPPVWQFVLASIVQLWLAIPFYKSAWASIKGGLANMDVLVT
                     IGTVSIYLYSVYMLFFSSHAAHGMAHVYFEAGVMVIGFVSLGKFLEHRTKKSSLNSLG
                     LLLKLTPTQVNVQRNGEWKQLPIDQVQIGDLIRTNHGERIAADGIIESGSGWADESHL
                     TGESNPEEKKAGGKVLAGALMTEGSVVYRAAQLGSQTLLGDMMNALSEAQGSKAPIAR
                     VADKAAAVFVPTVVGIALLTFIVTWLIKGDWTVALMHAVAVLVIACPCALGLATPAAI
                     MVGMGKAVKHGIWFKDAAAMEEAAHVDAVVLDKTGTLTEGRPQVAAVYYVPDSGFDED
                     ALYRIAAAVEQNAAHPLARAIVSAAQARGLEIPAAQNAQTVVGAGITAEVEGVGLVKS
                     GKAEFAELTLPKFSDGVWEIASAVTVSVNGKPIGAFALSDALKADTAEAIGRLKKHNI
                     DVYIMSGDNQSTVEYVAKQLGIAHAFGNMSPCDKAAEVQKLKAAGKTVAMVGDGINDA
                     PALAAANVSFAMKGGADVAEHTASATLMQHSVNQLADALLISQATLENIKQNLFFAFF
                     YNILGIPLAALGFLNPVIAGAAMAASSVSVLGNALRLKWVKID"
     gene            30616..31566
                     /gene="trxB"
                     /locus_tag="WNA25_000575"
     CDS             30616..31566
                     /gene="trxB"
                     /locus_tag="WNA25_000575"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFA9823396.1"
                     /translation="MSQHRKLIILGSGPAGYTAAVYAARANLNPVIITGIAQGGQLMT
                     TTEVDNWPADADGVQGPELMARFLAHAERFGTEIIFDQINAVDLQKRPFALKGDMGEY
                     TCDALIVATGASAKYLGLPSEEAFAGKGVSACATCDGFFYKNQDVAVVGGGNTAVEEA
                     LYLANIAKTVTLIHRRSEFRAEKIMIDKLMKRVEEGKIILKLESNLQEVLGDDRGVNG
                     ALLKNNDGSDQQIAVSGIFIAIGHKPNTDIFKGQLEMDEAGYLKTKGGTADNVGATNI
                     EGVWAAGDVKDHTYRQAITSAASGCQAALDAERWLGSQNI"
     gene            31722..32090
                     /gene="rpsF"
                     /locus_tag="WNA25_000576"
     CDS             31722..32090
                     /gene="rpsF"
                     /locus_tag="WNA25_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFA9823397.1"
                     /translation="MRHYEIVFIVHPDQSEQVPAMVERYKTMITEANGKIHRLEDWGR
                     RQLAYPINKIHKAHYVLMNIETTPEVVGELETAFRFNDAVLRHLTIKTKHAVTEASPM
                     LGGEKAKNLLVGAAEEAAAQ"
     gene            32091..32393
                     /gene="priB"
                     /locus_tag="WNA25_000577"
     CDS             32091..32393
                     /gene="priB"
                     /locus_tag="WNA25_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFA9823398.1"
                     /translation="MGFTNLVSLAALIEKAFPIRYTPAGIPVLDIILKHESWQEENGQ
                     QCLVQLEIPARILGRQAEEWQYRQGVYVHVEGFLAQKSRRSLMPMLRIQNIKEYKG"
     gene            32400..32630
                     /locus_tag="WNA25_000578"
     CDS             32400..32630
                     /locus_tag="WNA25_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFA9823399.1"
                     /translation="MARQSFKRRKFCRFTAEKIQEVDYKQVDLLKDFISENGKIIPAR
                     ITGTKAFYQRQLAVAVKRARFLALLPYTDQHK"
     gene            32647..33099
                     /locus_tag="WNA25_000579"
     CDS             32647..33099
                     /locus_tag="WNA25_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFA9823400.1"
                     /translation="MQIILLEKIGGLGNLGDIVTVKNGYARNFLIPAGKAKRATEANM
                     KEFEARRAELEAKQAEILADARARQEKLDGQTVTVAQKAGVDGRLFGSVTNADIAAAI
                     VAAGIEAVKANVRLPNGPLKAVGEYEVEVALHTDAVAKITVAVIAAAE"
     gene            complement(33722..34528)
                     /locus_tag="WNA25_000580"
     CDS             complement(33722..34528)
                     /locus_tag="WNA25_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFA9823401.1"
                     /translation="MWHWDIILILLAVGSAAGFIAGLFGVGGGTLIVPVVLWVLDLQG
                     LAQHPYAQHLAVGTSFAVMVFTAFSSMLGQHKKQTVDWKTIFAMMPGMIFGVFAGALS
                     AKYIPAFGLQIFFILFLTAVAFKTLHTGRQTASRPLPGLPGLTAVSTLFGAMSSWVGI
                     GGGSLSVPFLIHCGFPAHKAIGTSSGLAWPIALSGAISYLVNGLNIAGLPEGSLGFLY
                     LPAVAVLSAATIAFAPLGVKTAHKLSSAKLKESFGIMLLLIAGKMLYNLL"
     gene            complement(34529..35275)
                     /gene="pssA"
                     /locus_tag="WNA25_000581"
     CDS             complement(34529..35275)
                     /gene="pssA"
                     /locus_tag="WNA25_000581"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFA9823402.1"
                     /translation="METPTNTPQRSLRQNSIYLLPNSFTIAALFSAFYAITQSMHGRY
                     ETAAIAVFISMLLDGMDGRVARLTNSQSAFGEQLDSLADMVSFGVAPALIAYKWQLWQ
                     FGKIGYSVAFIYCACAALRLALFNTLIGKVDKRWFIGVPSPTAAALIVGLIWVNHSVE
                     KFPAVHWWALGITLFAGLSMIVQIPFWSFKEINIRRQVPFVGMLLAVLLLLLVTWEPS
                     LVLFLFFLGYSLSGYIMAARRFWKKYRKAD"
     gene            35858..36340
                     /locus_tag="WNA25_000582"
     CDS             35858..36340
                     /locus_tag="WNA25_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HFA9823403.1"
                     /translation="MMKKILAVSALCLMTAAAQAADTYGYLAVWQNPQDANDVLQVKT
                     TKEDSAKSEAFAELEAFCKGQDTLAGIAEDEPTGCRSVVSLNNTCVSLAYPKALGAMR
                     VENAVVITSPRFTSVHQVALNQCIKKYGAQGQCGLETVYCTSSSYYGGAVRSLIQHLK
                     "
     gene            complement(36387..37598)
                     /locus_tag="WNA25_000583"
     CDS             complement(36387..37598)
                     /locus_tag="WNA25_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease"
                     /protein_id="HFA9823404.1"
                     /translation="MNQLKLAVSGAQILFVAFGAMVLVPLLTGLNPALALLGAGLGTL
                     LFQITTKRKVPIFLGSSFAFIAPIIYSVGEWGLPSTMFGLFAAGFMYFVFAALIRWRG
                     LAAVHKLLPPVVTGPVIMVIGLSVAVAASSMAMGQADGKQVIDYTDSLILSGFTFAVT
                     AIVSVFGSRMMKLIPILIGVASGYVLALLMGLVDTTSIAHAPWFAVPHFETPQVNWQA
                     ALFMLPVAVAPAIEHIGGIMAIGNVTGKDYTKDPGLDKTLAGDGLGVCVAGLIGGPPV
                     TTYGEVTGAVMITKNSNPVIMTWAAVFAVCMAFFGKFNAFLASIPMPVMGGIMLLLFG
                     TIASLGIKTLIDAKVDLMLPKNLVIVSSVLTTGIGGMTLKLGSFSFVGVGLCAVLAIV
                     LNSLLPDPKES"
     gene            37820..40258
                     /locus_tag="WNA25_000584"
     CDS             37820..40258
                     /locus_tag="WNA25_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HFA9823405.1"
                     /translation="MTEKSHKKTAKGRAGSPSPTSARNKKADNGARGNKVSERLKAVK
                     ELQKTEAKKARPEHVVNLIADALWLMGLAATLYLAISLISFDMGDPSWSHSSPVVEDA
                     ANWGGLFGAYVADVGYYLFGWSFWWWIAAACVVLYKNFRLHAKQTENEAYNHKIAAAA
                     LFVLTVFSPVLEYFVLGGKYADSLPVGAGGMVGIRVGAVFAWLLGKSGSLLIILVVLL
                     LSLSLLVQISWLEFLNGAGRAVQNRLSALSGKVMALRKRRPNTKTDGVDTQNTRRMVK
                     EAKNITAKPVALPEGSSSNRKSVAVSVAPPPKIQPSLFEDNEVQQNGEYHKPTLNLLR
                     IPDSEPVSINPAELERTAELIESKLAEFGIGVQVVSATSGPVITRYEIEPAQGVKGSQ
                     IVALSKDLARSMSLQSVRIVETIAGKNTMGIELPNDKRQDVMLSEILSSPVFAEAKSK
                     LTVALGKDIAGTPVVGDLAKMPHLLVAGMTGSGKSVGVNGMIMSMLFKATPEEVRFIM
                     IDPKMLELSIYDGIPHLLCPVVTDMREAGQALNWCVAEMEKRYRLLSHAGVRNLEGFN
                     QKVEQAKAAGKPLLNPFSLNLDEPEPLEKLPMIVVVIDELADLMMTERKAVEQQIARL
                     AQKARAAGIHMIVATQRPSVDVVTGLIKANIPTRMAFTVQSKIDSRTILDQMGADELL
                     KYGDSLFLQPGSAEPTRLQGTFVSDGEVHQVVNYVKSQAPADYIEGLLSGEAALETAN
                     IVNPNADSDELFDQAVAYVLESKKTSISSLQRQLRIGYNRAANLMEALENAGIVSPSD
                     LNGSRKILAHKDHL"
BASE COUNT         9732 a        10018 c        10975 g         9543 t
ORIGIN      
        1 aaaccaatat ttaacagtcg gatagctccg cctgattaag tttttcggta atagttgtga
       61 ttatcaaata taattgccgc ccgaaatatc cgataagttt gtttgtttta tctttaattc
      121 cccaatactt aaaaaagaga gcgttttcat gaacaaaaaa cttgccttaa tgccgctgtt
      181 gattttgagc gcgttttcat ctgccgccga caatactccg cagcatggcg aactcggaca
      241 ggttcatgtc cgcgccgatg ccaagcgcgt caaagccgcg cattcttact ccatcgccag
      301 cgatggcgac ttgcgcgacc gcgtaaattt gggcgtattg ggtaaagcca acgcgtttac
      361 cgcgccgatt accgtcgtca actacgacga acaagccttc aacaataccg aggcgcgtac
      421 tttagtggat gccgtagcca aaaaagacgc ttccgtttga gagtttggcg gcgaaagcaa
      481 cacgctgacc ggtctgtatt tccgtggtta tcagcttgat gcgcgccaat tcagcgtaaa
      541 cggcttggca ggaatgtatg gcacgcaagg cacggcgagc gtgcaagtcg gcttcgcaca
      601 actgattaaa ggcgcgtcca ccgccgtaaa cggcataccc tgaaggcgcg gtatccggtt
      661 ccgtcaacat cgaaaccaaa aaagccgccg acgaaggcaa ccgcaaaatc ggtttggccc
      721 ggttcggcaa caaccgcgcc caaggtaggt tcgacttagg gcaacgcttc ggcgaaaaca
      781 aagcattcgg cgtgcgtgcc aacggcaaac tgcgccacgg cgacaccccg cgccacggtt
      841 acagagaaga caacaaagaa tttgccttaa atgccgatta tcgcggcgaa aaactgcgcg
      901 tgacgttcga ctccatctac gcgaaacgca aaatcaacgg cggccgcgcg cgtatgcagg
      961 atatccaaaa cgccggcgga cgcttgttcg acgcgcccga cggcaaaatc aacctgctgc
     1021 cttcttggaa ctggcaaaat accgtcggcg aaaccaatat gctgacgttt gaatgggatg
     1081 cgtttgacaa tacctaaatt accggcggta tcggctacaa caaagcgcgt tattacggta
     1141 cgctgatttc cccaaccgtt tgcggtacaa gcggtgcgag cagccaaacc aaaacctgca
     1201 tggctgccaa ccaataccac accggcacgg cacgcctgac cgaccaatat ttccgtactt
     1261 tgagtatgaa cctgaccgca cgcggcgaat ttgaaaccgg cccggtcacg cataactgga
     1321 gtacggcttt cgaccgcgtt atccgccagc ggaaaaccat aaacggcagc gggaacggca
     1381 acagcaagat agaagtgaag gcaaacgaaa atattgcgaa tcaattggca tcgttcaaag
     1441 ccgactatcc caattcttgg gcgaactcgg ccaacttgga tgccaatatc aaagtcaaca
     1501 gcttggcttt gtccgatact ttgggcttcg ttgataacaa ataccccctg actttgggcg
     1561 gacgtttcca agcggttgaa tacactgata agaagaaatc acagagcggc gatgccaaac
     1621 gtttcagccc gatgctgatg gcggcgtggg tgccgcaacc tgatttggtc gtgtgcggca
     1681 actatatgga agacttggag cgtgccgata tcaaaaccga tgattccggc gaaacgacaa
     1741 tggcgaagcc gcgcgtcagc cgccagtttg aaatcggcgt gcgtaaaaac tggggcgatt
     1801 ttgtaaccac gttgaatgcg ttccaaatca aacgcccggg ctactggcgc ggcaatacca
     1861 aaaaaggtac tgattttgca gcatataaag cagcaggagg cgcagccggc gacgagcaag
     1921 gtatggaacg caatcgcggt attgagttca atgcctatgc caacctgttg aacaaaaccc
     1981 tgcgcccgac tttcggcgtg atgtatctgc aatcgtccgt gaagaattat ccgaatgcgc
     2041 gcgatatgct ggttaacggc gtacaagtcg ccaacccgcg cgtgattgtc aaaacaggcg
     2101 ttgaacggga tacaccgttt gccaaaggtt tgagcttgaa cggcaatgtt tcgtatttcg
     2161 gcaagtctta tcaagacacg caaaaacaat acgccttccc gtcctatacc ttggttgacg
     2221 taggcgcgcg ctacaaaacc aagctgggca aaaacaccct gaccgtcagc agctcggtag
     2281 aaaacctgtt caacaaaaac tactggcagg tacagcgcgg acaatttgac cgtagtttcg
     2341 ccgtcgtcgg tctgccgcgt acttactggc tgaaggcgga attggatttc taagctgcct
     2401 ccgtttccga aaatgccgtc cgaacaggtt tcagacggca tttcttttaa agaaatatgg
     2461 cgggatatgc ttaaataatg ctattctagt acctttgaaa cctgcttgtt tgaagtgatg
     2521 aaaaaacctt tgatttcagt tgcggcagta ttgctcggcg ttgctttggg tacaccttat
     2581 tatttgggtg tcaaagcaga agaaagtctg acgcagcagc aaaaaatatt gcagaaaacg
     2641 ggctttttga ccgtcgaatc gcaccagtat gatcgaggct ggtttacctc tacggaaacg
     2701 acggtcatcc gtctgaaacc cgagttgctg cataatgcgc agaaatacct gccggataac
     2761 ttgaaaatag tgttggaaca gccggttacg ctggtaaacc atatcacgca cggccctttc
     2821 gccggcggat tcggcacgca ggcgcacatt gaaaccgagt tcaaatacgc gcctgaaacg
     2881 gaaaaagttt tggaacgctt ttttgggaaa caagttccgg tttcccttgc caataccgtt
     2941 tatttcaacg gcagcggtaa aatggaagtc agtgttcccg ctttcgatta tgaagaactg
     3001 tcgggcatca ggctgcactg ggaaggcctg acgggggaaa cggtttatca aaaaggtttc
     3061 aaaagctacc gcaacagcta tgatgcgccc ttgttcaaaa tcaagctggc agacaaaggc
     3121 gatgccgcgt ttgaaaaagc gcatttcgat tcggaaactt cagacggcat caatccgctt
     3181 gctttgggca gcagcaatct gactttggaa aaattttcgc tcgaatggaa agagggtgtc
     3241 gattacaacg tcaaattgaa cgaactggtc aacctcgtta ccgatttgca gatcggcgcg
     3301 tttatcaatc ccaacggcag catcgcacct tccaaaatcg aagtcggcaa gctggctttt
     3361 tcaaccaaga ccggggaatc gggcgcgttt atcgacagcg aagggcggtt ccgtttcgat
     3421 acgttggtgt acggcgatga aaaatacggc ccgctggaca tccatatcgc tgccgaacac
     3481 ctcgatgctt ctgccttaac cgtattgaaa cgcaagtttg cacaaatttc tgccaaaaaa
     3541 atgactgagg aacaaatccg caatgatttg attgcggcag tcaaaggcga tgcttccgga
     3601 ttatttaccc atgacccggt actaaatatc aaaattttcc gtttcaccct gcctcaggga
     3661 aaaattgatg tgggcggaaa aatcatgttt aaaggcatga agaaggaaga tttgaaccaa
     3721 ttgggactga tgttaaagaa aaccgaggca aacatcagaa tgagtattcc tcaaaaaatg
     3781 ttggaagatt tggcggtaag tcaggctgga aatattttca gtgtaaatgc cgaagatgag
     3841 gcggaagcca gagcaagcat tgccgatatt aatgaaacat tgcgcctgat ggtggacagt
     3901 acggtccaaa gtatggcaag ggaaaaatat cttactttag acggtaatca gattgatacg
     3961 gtcatttccc ttaaaaacaa cgccctgaag ttaaacggga aaacgctgca aaatgaaccc
     4021 gatcctgatt ttgacgaggg agatatggtt tccggccagc cgcattaatg ccgtttatgc
     4081 caaatgccgt ctgaaatgtt cagacggcat ttttgtcggt ctcgaaatag gatggatttt
     4141 catgtttcaa aaacaattga ttgtctctaa tccgatatca ggacacgctt ccaatgttta
     4201 gatatggaat tcggacggga taggggaact attgaaatcc agttgaaatc tgttattgga
     4261 aagcatggag gaaaaataaa aggctgtatt ttaatatagt attgataggc atgcttagct
     4321 tcaaggtatt caatagtgta ggtgtctgcc taactggaaa ataatcgtct tttcccttgc
     4381 aaccagttaa aattgacaga tggttaaaac acagccaaat aaaaatgccg cctgaagggt
     4441 tcagacggca tttgttcgga taaaccttat cggctgctta gagcatcagg tttatttttt
     4501 cttttgagga ggcaggtcgg tacatacacc caaagccact tccgcagcca taccgatgga
     4561 ttcgcccaag gtcgggtgcg ggtggatggt tttgccgatg tctgccgcgt cgcagcccat
     4621 ttcgatggca aggcagactt cgccgatcat gtcgccaccg ttcggaccga caatgccgcc
     4681 gccgatgatg cggccggttt cggcatcgaa aatcagcttg gtaaagccgt tgtcgcaacc
     4741 gttggcaatc gcacgaccgg aagccgccca tgggaagttg gctttggtga ttttgcggcc
     4801 tgatgctttg gcagacaatt cggtttcacc gacccatgcc acttcggggg aagtgtaggc
     4861 aacgccggga ataacgcgtg cgtcgaagta ggctttgtgg ccggcacagt tttcagcggc
     4921 aacgtgacct tcgtgaacgg ctttgtgtgc caacatcggt tgaccgacga tatcgccgat
     4981 agcgtagatg tgcggtacgt tggtgcgcat ttgtttgtca acttcgatga agccacgatc
     5041 ggtaacggca acaccggctt tttctgcgct gatgagtttg ccgttaggcg cacggcctgc
     5101 cgcgaccagt acggcatcgt aacgttgcgg ctctttaggt gcatttgcac cttcaaaggt
     5161 aacgtaaacg ccgtcttctt tcggctcgac tgcaacggtt ttggtgttga ccataatgtt
     5221 gtcaaaacgg tattcgtttt gtttttgcca tactttaacc aagtcgcggt ctgcgccttg
     5281 catcaggccg tccatcattt caaccacatc caggcgagaa cccagcgtgc tgtaaaccgt
     5341 acccatctcg aggccgataa tgccgccgcc gataatcagc agtttgcccg gtacttcttt
     5401 cagtgccagt gcgccgctgg aatcgatgat gcgcggatct tcaggaatga aaggcagttt
     5461 ggttacgcgg ctgcctgctg caatgataca gtttttgaag gcaacgattt ttttctcgcc
     5521 ggtaggggct gcctgttcgt acgcgtcgcc ggcagtcagc gacacttcca agtggtgagg
     5581 atccaagaat tgcccgtcgc cttggataac gtccacttta cggcttttcg ccatacctgc
     5641 caaaccaccc gtgaggcggg aaaccacgcc gtctttatag gcgcgaagca tatcgatgtc
     5701 gagttccggt tcggggtatt tgataccgtt ggcagccaag tggcgcactt cgtcgataac
     5761 ggcggcattg tgcaacaagg ctttggaagg gatacagccg acgttcaggc aaacgccgcc
     5821 caaagttttg taacgctcga cgatggcgac tttcaagcct tcatcggcag cggcaaatgc
     5881 agcggagtaa ccgccgggac cgccgcccaa tacgaccacg tcgtactcgg catcggcagc
     5941 actgccgaat tgcgcggctt gcggagcagg agcggcagct ttgggtgctt cttgcgccgg
     6001 ggcggcagcc gcttcggctt taggggcggc agccgcacct tcggcttcga cgacgacaat
     6061 caaaccgcct tcggagattt tgtcgccgac tttgactttc acttctttga tcacgcccgc
     6121 aacttcggca ggcacgtcca tcgtcgcttt gtcggtttcc aaggtaatca gggtgtcgtc
     6181 aacggcgatg gtgtcgccaa cgttcacttc aaccgcgata atatctacat tttcgtgtcc
     6241 gccaatgtcg ggcactttca attcaactaa agccattata ttgacctttc aacttagtta
     6301 atttctatca actctttagc aataatcact tcttcaggaa tacagccaca atcttcagct
     6361 ctgattgtta cttccttctc atctgggttt tctggatgtt caacctcata ttcaaaaata
     6421 gaatattgac caaacaaatc tctatttaaa acatatatat agccattttc gatgccggag
     6481 cttgttgcaa atttcttggc aatttccttg tctgttgtcg tagatatata acatccgtca
     6541 tatagatctg tttcaatttg atgggcgtaa actgcattct tcacacttgg accatgtgta
     6601 gctttaccat catatttaaa cttaccatca taacgaattg caacttcagc tttattacct
     6661 ttaggtttta actgtccatt attttgctca tcttgttggc aactaatgcc tctatataaa
     6721 taagaaattt cccataagtt attaatttaa agtttgaatt taacaccatc actcatcaat
     6781 ctcaagcaat ccaaacatta tcagactgct tgagagggat gttttatttt ataaggtaat
     6841 gcggcggaag tctttcaaca ggttcgccag gaatacggtg aagcgcatac cggcggcacc
     6901 gtcgatgacg cggtggtcga aggacaggct caacgggcac atcaggcgcg gggcaaactc
     6961 tttgccgttc caaaccggtt tgatttggga tttgcacacg cccaagatag cgacttcggg
     7021 agcgttcaca atcggcgtga aacctgtgcc gccgatgccg cccaagctgg aaatggtaaa
     7081 gcacgcgcct tgcatttctt gcggtttgag cttgccttcg cgggcttttt tggacaattc
     7141 ggtcaattct tggctgattt gtttcaagcc tttttgatcc acgtctttga tgacgggaac
     7201 aaccaagccg ttcggcgtat cggctgcgaa accgatgttg aagtagtttt tcagcaccag
     7261 gttgtcgccg tccaaagaag cgttgaattc ggggaaggct ttcagcgcgg aaacggaggc
     7321 tttgatgatg aacgccaacg gggacagttt cacgccttcg cgttcccatt ctttgttcag
     7381 ctgtttgcgg aattcttcca gctcggtcat atccgcttct tcgtgtacgg taacgtgcgg
     7441 aatcaccacc cagttgcggg acaggttttg accggagatt ttcttaatgc gggacaattc
     7501 tttaacttcg acattgccga atttggagaa gtccacttta ggccacggca gcaagtccag
     7561 accgccgccc aaagatgcgc cggctgcggc aggttttgcc gcgccgccct gcattacgga
     7621 tttcacaaag gctttgatgt cgtctcccat gatacggcct ttcaagccgc tacctttgac
     7681 ttggcccaaa tccacgccca attcgcgcgc cagtttgcgt gcggaaggac cggcgtgtgc
     7741 tttggcgaaa gcggcttcat cgattttggc agcggcaggc gaggcagatg taggcacggc
     7801 ggcgggtgcg gcagcttgag cggcaggcgc aggagctgct gcggcagagc cggcggtttc
     7861 cacttcgata atggcagagc cttcggacac tttgtcgccg acttttaaga atacggcttt
     7921 aacgacaccg gcagcggtac aaggtacgtc cattgtcgct ttatcggttt ccaaagtaat
     7981 cagcgtgtcg tcttcggcaa cggtgtcgcc aactttgatt tcaacggcga ttacatctac
     8041 atcggtatgg ccgccgatat cgggaacggc tacttgaacg gttgcaccgc ctgcggcagc
     8101 gggtgcaggt tgtgcttcgg cggcagcagc cggggcggct tcggcagcgg cggcaccggt
     8161 ttcaacggtc aaaattacgc cgccttcgga gattttgtcg ccgactttga cttttacttc
     8221 tttcacgaca ccggccgcat cggcaggcac atccatcgtg gctttgtcgg tttccagtgt
     8281 aatcagggtg tcgtcaacgg cgatggtgtc gcccgcttta acttctacgg cgatgatgtc
     8341 gacgctttcg tgaccgccga tatcggggac tttgatttct acgatactca tttgagttcc
     8401 tttaatcttt ttcagttgga tgccgtctga aagctgcttc aggcggcatc ggtacggttt
     8461 gtctgttcaa accattcggg cattcggttt tcagacggcc tgatgttggc atgaaacaaa
     8521 caggccgtct gaaacatcaa tcagcgtttc cagctagggg ctgaatcggc tttgatgccg
     8581 tatttctcaa tggcttgttg aacggtttct ttgctgactt tgccttgttc cgccaatgcg
     8641 gccaatgctg ccacggcaac gttgtagcgg tccacttcaa agaagcggcg caggttggcg
     8701 cggctgtcgg aacggccgaa accgtcagtg cccaagacat ggtagtcgtt cgggatgtac
     8761 gcgcggatac ggtcggcata gctgcggata tagtcggtag cggcgataac cggaccgtca
     8821 tgaccttgca gttgggaagt aacgaaaggc actttttcag cttccagcgg atgcaggcgg
     8881 ttgaagcgtt cggtttcgat ggcgtcgcgg tgcagcaggt tgaaggacgg gcaagaccag
     8941 atgtctgctt ccacgccgaa gtcggctttc agcagctcgg caccggcaat cacttcttgc
     9001 agaatcgtac cggaacccat caattggact ttcttgtcgc ctttgccgcc ggctttcagc
     9061 aggtacatac ctttgaggat gtcttgttcc gcaccttccg gcatatcggg atgggtgtag
     9121 ttctcgttca tcagggtgat gtagtagaac acgtcttcat tattggcata catacggcgc
     9181 agaccgtctt gtacgatgac ggccacttcg tattggaagg tcgggtcata agatacgcag
     9241 ttcggaatca ggtcggcctg gatgtggctg tggccgtctt cgtgttgcag accttcgccg
     9301 ttcagcgtcg tacggccggc agtaccgccc agcaggaagc cgcgcgcgtg catatcgccc
     9361 gccgcccaag ccaagtcgcc gatacgttgg aaaccgaaca tggagtagta gatgtagaac
     9421 ggaatcatcg cgaagtcgct gttggcgtag ctggtcgcag ccgcaatcca gtcggccatc
     9481 gcgcccggtt cgttaatacc ttcttgcaag atttgaccgt cgacggattc tttgtagaac
     9541 atcagttggt ctttatcttg aggcgtgtat tgttggcctt tgggattcca aataccgtat
     9601 tggcggaaca taccttccat gccgaaagta cggctttcgt cgggaacgat aggtacgacg
     9661 cgtttgccga tttttttgtc tttcagcaaa gtggacagga tgcggacgaa tgccatcgtg
     9721 gtcgagaact cgcgttcacc gctggatttg agttgtgcgt cgaatgccga cagctcgggc
     9781 acttccaata cttcctgcgt cggtttgcgt tgcggcaggt agccgcccaa agcatcgcgg
     9841 cgtgcgtgca ggtatttgta ttcttccgta tcgggggcaa aagtcaggta aggcagatcg
     9901 ccgctttcga tttgttcgtc ggtaaccgga atgtcaaagc ggtcgcggaa ttgtttcagg
     9961 gacgctttgt ccattttttt ggcttggtgg gcaacgttct gaccttcgcc ggatgcgccc
    10021 ataccgtaac ctttaatggt tttcgccaag ataacggtag gtttgccgtc ggcatggttc
    10081 gctgcgcggt cgtaggcgtt gtacactttt tgaggatcgt ggccgccgcg gttcaatgcc
    10141 cagagttgct catcggtcat atcggcaacc aatgctttca gttcgggcgt attgaagaag
    10201 tgttcgcgca catacgcgcc gtctttggat ttgtaagttt ggtagtcgcc gtccaaacat
    10261 tcttccatac gttggcgcag gataccgtct ttgtctttcg ccaagaggcg gtcccaacgg
    10321 cggccccaaa tgactttgac gacattccag ccggcaccgg caaagttgcc ttccaattct
    10381 tggatgattt tgccgttgcc gcgcaccgga ccgtccaagc gttgcaggtt gcagttgatg
    10441 acgaaaatca ggttgtccaa gccttcgcgt gcagccagcg cgattgcacc ttgggattcg
    10501 ggttcgtcca tttcgccgtc gccgcagaaa caccatactt tacggccttt ggtttttgcc
    10561 aagccgcgtg attccaagta tttcaggaaa cgcgcctgat aaatcgccat gatggggccc
    10621 aagcccatgg atacggtcgg gaactgccag aagtcgggca agaggtgggg gtgtggatag
    10681 gaaggcagac cgtgtccgtc cacttcttgg cggaagttgt tcagctggtc ttcggtcaga
    10741 cggccttcga cgaatgcgcg tgcatagatg cccggggcaa cgtgaccttg gaagaagacc
    10801 aaatcgcctt cttcaccttc acctttggct ttccagaagt ggttgaaacc gacttcgtac
    10861 atggtggcgg cagattggaa agatgcgatg tgcccaccca gttccaaatc tttcttgccg
    10921 gcgcgcaata cgatggcggc ggcgttccag cgtacgaatg cgcgaatgcg gtgttcgatg
    10981 ttttggtcgc ccggaatgcc tttttcgttt tcaaccgaaa cggtattcaa atacggggtg
    11041 gtcgtgccgt ggggcatacg tacgcctttg tcgcggcagt atttgagcag gttttccaag
    11101 aggtattgcg cgcgttcgcc gccttcatat tcgaggacgg agcttaacgc gtccagccac
    11161 tcttgggttt cgatagggtc aacatcgtgt aattgggtgg acatagtatc tatcctttat
    11221 gttgagtgtt taatcggaag ggagggtgta gccaacccct ttattttcgt ttgtgaaaaa
    11281 taataagcaa aaacgaaaat ttctaaatca aaccgaaaca aaataattta agtttaatgt
    11341 tttcaaaaaa ttataaaagt cttggaagca agggaaaact tgaaaaaaaa aatgttcggt
    11401 ctgctccgca cgaaatccta tcagcaatcg ggtctgctgg caaataacca tgcttttaaa
    11461 agcaggtttt atactgaagt tggcgtttgg taaggacagg tctttttgat tgcgaaaaca
    11521 ttctgtttaa ttttggttat attttttcgg aattgtggga tatcgaatcg ttatgtctct
    11581 gtcggcaatt ttcctattgc agaaaatcta tacacaagct ttgtggacaa ccgtgttgat
    11641 aagccacggc ttgtgttaaa aaagtcttac gcaacaaatg tacgcttatt ttgattaaaa
    11701 attaggcaaa tttaaatcta taaaaatcat gtaattatgt tcaatatgat gtttctggtc
    11761 atgtttttgc cgtctgttat ggggtttgtc ccgcgtttgg ggcagggggt gaattttgga
    11821 ggttggtgca aaacctgctt ttttcgcgtt acaatctgca cccgatttac ttttgaatct
    11881 gcctgtttgg aaaaatactt ttatgaaagc cagccaattc tttatctcta ctttaaaaga
    11941 agcccctgcc gaagccgcgt ttgccagcca caagctgatg attcgcgccg gtctgattaa
    12001 agccaacgcg tccggtcttt atacttggat gccgatgggg ctgcgcgtgt tacgcaaagt
    12061 cgaaaacgtc gtgcgcgagg aaatggcgcg cgcgggcagc gtggagctgc tgatgccggt
    12121 ggtgcagcct gccgaactgt ggcaggaatc cggccgctgg gagttttacg gtaaagaact
    12181 gctgcgcctg aaagaccgcc acgaacgcga tttctgcatg ggcccgacct gcgaggaagt
    12241 catcgccgac atcgtgcgca aagaaatcaa cagctacaaa caactgccga aaaattttta
    12301 ccacatccaa accaaattcc gcgacgaagt gcgcccgcgt ttcggcgtga tgcgcgcgcg
    12361 cgaatttgtg atgaaagatg cttattcctt ccacgccgac tacgcctcgc ttcaggcgac
    12421 ctatgatgcc atgtatgacg cttactgccg catctttacc cgtctgggtt tggcgttccg
    12481 ccccgtcgcc gcagacaccg gcagcatcgg cggtacgggt tcgcacgagt ttcaagtgtt
    12541 ggcggaaagc ggcgaagatg tgattgcata cagcgacact tccgattacg ccgccaatat
    12601 cgagttggca ccgaccttgc cgcttaaagg tgaacgtgcc gccgctcagg ctgtgttgac
    12661 caaagtacat acaccaaacg tcaaaaccat tgagtctttg gttgaattcc tgaatattcc
    12721 ggttgaacaa accctcaaat ccatcgtggt tgaaggcgaa aacgaaggcg aactcgtcct
    12781 actgctgttg cgtggcgacc atgagtttaa cgacatcaag gcagaaaaac tggcgggcgt
    12841 aaaatcgcca ctgactatgg caagccctgc cgcgattgtt gaacaattcg gcgcaaacgg
    12901 cggttcgctc ggccccgtcg gcttcacagg caaagtctat gccgatttcg ctaccgaaaa
    12961 aggcgcggac tgggttatcg gcgcaaacga agacgactac cactataccg gcttcaactt
    13021 cggccgcgat gccgccgagc ctgaattcgt cgatttgcgt aatgtggtcg aaggcgacga
    13081 aagccccgac gggcaaggcc gtctgaaact ggcgcgcggc atcgaagtcg gacatgtctt
    13141 ccagttgcgc ggcaaatata cccaagctat gaacgtcagc ttcctcgaca ataacggcaa
    13201 atcgcaaatc atggaaatgg gctgctacgg catcggcatc acccgcgtcg ttgccgccgc
    13261 catcgagcag aataacgacg aaaaaggcat catttggacc aaagcgatgg cgccgtttga
    13321 agtcgtcatc gtgccgatga actacaaaaa atccgacact gtgcgtgaag ccgccgacag
    13381 aatctatgcc gaattgctgg cggcaggcgc ggatgtgctg ctggacgacc gcgacgaacg
    13441 cgcaggcgta ttgctgaacg attccgagct tctcggtatc ccgcaccgca tcgtcatcgg
    13501 cgaccgcgcc ttgaaagaag gcaatgtcga atacgccgaa cgccgcgaca acgaagcgca
    13561 ggcagttgca atcggagaaa ttgttgcgcg tgtaacagct tcattaaatg cgtaagtctg
    13621 gtcaaatgcc gtctgaaaat aatttcagac ggcattttta ttttggggga aagtgaagtg
    13681 gcttcagtat ttttagaagg agaagcagtc gaggtcggca atattttctg tatcggcagg
    13741 aactatgccg cacatgtcga agagttaaaa aacgaaatac cttcagaacc tgtggtgttt
    13801 atgaagccgt caggcagcat tttgaacagc ggcggtacaa tcttgctgcc cgagttcagt
    13861 agggatgtgc agtttgagtg cgaactagtg ttgctgattg gccgggattc agacggcacg
    13921 ggcgagggtg aagatatttt gggatgcgtg gcggggtacg gcgtgggttt ggatttgacc
    13981 gcgcgggaca tccaatgccg tctgaaggcg caggggctgc cctggctgaa ggcaaaaggg
    14041 ttcaggcatt ctgcgtgcgt gtcggatttt gcggcggcag acagaatcgg caatcaagat
    14101 aaggtgttgt tttcattaaa acaaaacggc gtattgaaac agcgcgggga gactggtttg
    14161 atgatttacc cgattcggga aattttacac aaactggcgg cggattacgg tttggggaaa
    14221 ggggatttgg tgtttaccgg aacgccctcc ggcgtgggcg caatcggtgc gggggacaat
    14281 ttggcattgg agctggacgg cttggtgtgc gcctctttca cgatagggtg ttaaccgcgc
    14341 cgcttaacct tcacgcccgt tgaaacattc cgccgggccg ccctcgagaa aaccgtgcag
    14401 gatggcttgc gccgccactt ggtcgagcac cgatttgcgt tttttgccca agacctgtgc
    14461 ttccgaaagc aggctttcgg catagacgga cgacagccgt tcgtcaaccc aatagacggg
    14521 gagattgaac ctgccgttca gcctgcgtcc aaacttgcgc gacaggtgcg tcatttcatg
    14581 ttccgtgccg tcggcatgca cgggcagtcc gacgacaaaa taacgcggct gccattcttg
    14641 aaccagcttg gcgattgccg cgaatttttc atcgttgctg ccgccggtaa cggtggccag
    14701 cggatgggac agccccaatt ccgcatcgcc ctgcgccacg ccgatgcgcg cttcgccgaa
    14761 gtcgaatgcc agtgccgttc cttttggaat tttatgcatg gccggctccc gaaaacaggg
    14821 cgagcgggtc gatgccgagt ttggcgaatg cggcggcgta acggtgttcg taggggatgt
    14881 cgaacaggat gtgttcgtcg gcgggaacgg tcagccacgc attgtcggca agttcgcgtt
    14941 cgagctgccc tttgctccag ctcgaatagc ctatgctgat caaggctttg tcaaccgcgc
    15001 cttcgcgtga aatgttttca agcacatcgc gggaagaggt cagcgcgatg ccgtctgaaa
    15061 cgccgatgct gctttgccag ttgccgatcg gagtgtggac gacgtaaccg cgctcgacct
    15121 gcaccggccc gcccatcatc acgctgtcgt gctgcatccg catggggatg tttttgccgg
    15181 tggcggaaaa aatcatgtcc atcgtaatcg gagagggttt gttgatggcg ataccgagtg
    15241 cgccgtcttc atcgtgtttg cagatataga cgaccgattg tgaaaaaaac gcgtcttcca
    15301 tatcgggcat ggcgacgaga aagtggttgg acaggttcat atcagcgttt gtcgggattt
    15361 tttatcgggt tggaaataat gcaggcgccg gcaagcagtt tgccggaagt atcggcttgc
    15421 accccgtcgg tttgcggtat gccgctgccg aaacaggaaa ccgcgcttaa ggaatttttc
    15481 ggcgatcctt ggacgatttt atcgctgtaa accaaatagg cgaaggcttt gcgtttgggg
    15541 tcgtaataac ggacaatctg ccggctcttg aacgcgaagc ccgtaccgcg cttgaaaact
    15601 tctttcggtt tgcgcacggc ggtttcgtca aaagaaatcg aagatgccgt ctgaacgcac
    15661 gaaaccgatg cgtcggacgc gtcctcttcc aaattgacca tttccttcaa gccgcctttt
    15721 tttgcatacg aaatataaca ggcaacccct tgaacgtcgg gatcgtcgaa tccttccact
    15781 tcgatacggt cgtttttgcc caacatgttg aaaacggtac ttgcccgtcc gattttatcg
    15841 gtttcgccgc tgccgcaggc agtcggcagg acggcggcag acagcagtag cagtctgttc
    15901 atcaaaattt ccttatcggt tgaatccccg ttttccggaa ggcggcaggg tttgctttgt
    15961 ttggaggggc gcacgctccc aaatcgggca acatacggta cgcagcttac atgtgctacc
    16021 ctgtgtgtcg gaacacaata tccgttgcag cattgaaaat atgccgcaca atataaggct
    16081 gtttgagccg attgcaagta tccgcgcaaa gaaggtgcaa gattgggcaa acggcatttt
    16141 cagacggcat aactgacagt ataatccgaa catccggccg ctttcgcgcg gccttcagca
    16201 ttatccgatt ttttctgaaa gccgaaccat gcaatacaaa cccctcctgc ttgccctgat
    16261 gcttgtcttt tccgcccccg ccgttgccgc ccacgacgcg gcacacaacc gttccgccga
    16321 agtgaaaaaa caggcgaaga acaaaaaaga acagcccgaa gcggcggaag gcaaaaaaga
    16381 aaaaggcaaa aatgccgcag tgaaagataa aaaaacaggc ggcaaagagg cggcaaaaga
    16441 gttcaaaaaa accgccaaaa accgcaaaga agcagagaag gaggcgacat ccaggcagtc
    16501 tgcgcgcaaa ggacgcgaag gggataagga atcgaaggcg gaacacaaaa aggcacatgg
    16561 caagcccgtg tccggatcca aagaaaaaaa cgcaaaaaca cagcctgaaa acaaacaagg
    16621 caaaaaaggg gcaaaaggac agggcaatcc gcgcaagggc ggcaaggcgg aaaaagacac
    16681 tgtttctgca aataaaaaag cccgttccga caagaacggc aaagcagtga aacaggacaa
    16741 aaaatacagg gaagagaaaa atgccaaaac cgattccgac gaattgaaag ccgccgttgc
    16801 cgctgccact aatgatgtcg aaaacaaaaa agccctgctc aaacaaagcg aaggaatgct
    16861 gcttcatgtc agcaattccc tcaaacagct tcaggaagag cgtatccgcc aagaacgtat
    16921 ccgccaagag cgtatccgtc aggcgcgcgg caaccttgct tccgtcaacc gcaaacagcg
    16981 cgaggcttgg gacaagttcc aaaaactcaa taccgagctg aaccgtttga aaacggaaat
    17041 cgccgctacg aaagcgcaga tttcccgttt cgtatcgggg aactataaaa acagccagcc
    17101 gaatgcggtt gccctgttcc tgaaaaacgc cgaaccgggt cagaaaaacc gctttttgcg
    17161 ttatacgcgt tacgtaaacg cctccaatcg ggaagttgtc aaggatttgg aaaaacagca
    17221 gaaggctttg gcggtacaag agcagaaaat caacaatgag cttgcccgtt tgaagaaaat
    17281 tcaggcaaac gtgcaatccc tgctgaaaaa acagggtgta accgatgcgg cggaacagac
    17341 ggaaagccgc agacagaatg ccaaaatctc caaagatgcc cgaaaactgc tggaacagaa
    17401 agggaacgag cagcagctga acaagctctt gagcaatttg gagaagaaaa aggccgaaca
    17461 ccgcattcag gatgcggaag caaaaagaaa attggctgaa gccagactgg cggcagccga
    17521 aaaagccaga aaagaagcgg cgcagcagaa ggctgaagcg cgacgtgcgg aaatgtccaa
    17581 cctgaccgcc gaagacagga acatccaagc gccttcggtt atgggtatcg gcagtgccga
    17641 cggtttcagc cgcatgcagg gacgtttgaa aaaaccggtt gacggtgtgc cgaccgggct
    17701 tttcgggcag aaccggagcg gcggcgatgt ttggaaaggc gtgttctatt ccactgcgcc
    17761 tgcaacggtt gaaagcattg cgccgggaac ggtaagctat gcggacgagt tggacggcta
    17821 cggcaaagtg gtcgtgatcg atcacggcga gaactacatc agcatctatg ccggtttgag
    17881 cgaaatttcc gccggcaagg gttatacggt cgcggcagga agcaaaatcg gcacgagcgg
    17941 gtcgctgccg gacggggaag aggggcttta cctgcaaata cgttatcaag gtcaggtgtt
    18001 gaacccttcg ggctggatac gttgatcgga ttgccgaaac ggcaggcttt atgcaaatcg
    18061 tgccgatatg gtgcgctgtt ccgtgaagtt tgccgtttgc cgtacctgtg ttttccggtt
    18121 tttggaaatc aagagagaaa gttaaagaag aatgtcgaaa cctgttttta agaaaatcgc
    18181 actttatact ttgggtgcaa tcagcggcgt ggccgtaagt ctggcggtgc agggttttgc
    18241 cgccgagaag gacgggcggg ataacgaagt cctgccggtg caatccatcc gtacgatggc
    18301 ggaggtttac ggtcagatta aggcaaacta ctatcatgac aaacccgatg ccgatttgtt
    18361 tgaaggtgcg atgaagggta tggtggccgg tttggatccg cattccgaat atatggataa
    18421 aaaaggttat gccgagataa aggagtccac cagcggcgaa tttggcggct tggggatgga
    18481 aatcgggcag gaagacggtt ttgtcaaagt ggtttcgccg attgaggaca cgcctgccga
    18541 acgggcggag gtgaaaagcg gcgatttcat tgtgaaaatc gataatgttt cgacgcgcgg
    18601 tatgacggtc agcgaagcgg tgaaaaaaat gcggggcaag ccgggtacga agattacttt
    18661 gacgctgtcg cgcaaaaatg ccgacaagcc gatagtcgtc aacctgaccc gtgccattat
    18721 taaagtgaaa agcgtccgcc atcacctgat cgaacccgat tacggctata tccgcgtgtc
    18781 gcagttccaa gagcggacgg tcgaaagcgt caataccgcc gcaaaagagc tggtaaagga
    18841 aaataaagga aaaccgctca aggggctggt gttggatttg cgcgacgacc ccggcgggct
    18901 tttgaccggc gcggtcggcg tgtcggcggc gtttctgccg tctgaagcgg tcgtcgtcag
    18961 caccaaggga cgcgacggca aagacggcat ggtactgaaa gccgttcccg aggattatgt
    19021 gtacggtatg ggcggcgatc ctttggcggg tattcctgcc gagttgaaaa cgattccgat
    19081 ggcggtattg gtcaattccg gttcggcttc cgcgtcggag attgtcgccg gcgcattgca
    19141 ggaccacaaa cgcgcggtca tcgtcggtac gcagagcttc ggtaaaggtt cggttcagac
    19201 tttgattcct ttgtccaacg gcagcgcggt caagttgacg accgccctgt attacacgcc
    19261 gaacgaccgt tccattcagg cacaggggat tgttcccgat gtcgaagtaa aagataagga
    19321 acgtactttt gaaagccgcg aggcggacct ggtcggacac atcggcaatc ccttgggcgg
    19381 cgaggatgtg aacagtgaaa cccttgccgt accgcttgaa aaagatgcgg ataagcccgc
    19441 tgcaaaagaa aaaggtaaaa agaaaaagga cgaggatttg tcttcaaggc ggattccgaa
    19501 ccctgcgaaa gacgatcagt tgcgtaaggc tttggatttg gtcaagtcgc ccgagcagtg
    19561 gcagaagtct ttggggctgg cggcgaaaaa gccggtttca aataaagata aaaaagataa
    19621 gaagtaggtt ttgacgatgc cgtctgaaag ggtttcagac ggcatattat gtttgaaagg
    19681 acaggttatg agcgattttt taccggaatt tgaaccgtgc ggcatttata gtggattaac
    19741 aaaaatcagg acaaggcgac gaagccgcag acagtacaaa tagtacggca aggcgaggta
    19801 acgccggacc ggtttaaatt taatctacta tacttgggta gcgattgggt ttcagacggc
    19861 atacattcag gatttcaccg ccttataccg tctgaaagac gtttctgacc catccctact
    19921 acacgctttg tccgcaaacg tatcacaggc aagattatgg aagtcatccg atacccaaat
    19981 tcccccttca aactccacca acccttcccg cccgcaggcg accagcccac cgccattgcg
    20041 ggcctgctcg aagggctttc agacggcctt gcctaccaaa ccctgctcgg cgtaaccggt
    20101 tcgggcaaaa cctacacgat ggcgaacgtc atcgcgcaaa gcggcagacc cgccatcatt
    20161 atggcgcaca acaaaaccct tgccgcccag ctttatgccg aaatgcgcga gtttttcccc
    20221 gaaaacgcag tggaatattt cgtgtcgtat tacgactatt accagcccga agcctatgtg
    20281 cccagccgcg atttgttcat cgaaaaagac agcgcgatca acgaacacat cgagcagatg
    20341 cgcctttccg ccaccaaaaa cctgatgacg cgcgacgacg tgattatcgt cgccaccgtg
    20401 tccgccattt acggtatcgg cgacccgacc gagtatcaac aaatggtgct gtccgtaaaa
    20461 gaaggcgaca ccatcgagca gcgcgacatc atcgccacgc tcgtttccat gcagtacgaa
    20521 cgcggcgatt tggacttcaa acgcggcagc ttccgcgtgc gcggcgacgt gattgacgtg
    20581 taccccgccg aaagctccga aaacgccttg cgtatcagcc tgtttgatga cgaaatcgac
    20641 cgcctcgata tgttcgaccc gctttcaggc agcctgcacc agcgtgtcgg acgctacacc
    20701 gtcttcccgt ccagccacta cgttaccccg cgcgacaccg tattgcgcgc ctgcgaatcc
    20761 atcaaagaag aattgcgcga acgcatcgaa tttttcgctc gcgaacaacg ccccgtcgag
    20821 caacaacgca tcgaacagcg cacccgtttc gacctcgaaa tgctctacga aatgggcttc
    20881 tgcaaaggca ttgaaaacta ctcccgccac ttctccggta aaaaagaagg cgaaccgccg
    20941 cccacgctga tggactacct gcccgacaac gccatcatgt tcatcgacga aagccacgtt
    21001 accgttaccc aaatcggcgg catgtacaaa ggcgacgcat tgcgcaagca aaacctcgtg
    21061 gactacggct tccgcctgcc ttccgcccgc gacaaccgcc cgctcaaatt ccacgaattt
    21121 gaaaaagtca tgccgcaaac cgtcttcgtt tccgccaccc ccgcgaaata cgaagaagaa
    21181 cacgccggac aagtcgtcga acaagtcgtc cgccccacag ggctggtcga cccccaaatc
    21241 atcatccgcc ccgtcgccac ccaagtcgac gatttaatga gcgaaatcaa cgaccgtatt
    21301 caaaaaggcg aacgtgtgct cgttaccacc cttaccaaac gcatggcgga gcaactcacc
    21361 gactattaca gcgaactcgg catcaaagtg cgctacctgc acagcgacat cgacaccgtc
    21421 gaacgcgttg aaatcattag agatttgcgg ctcggcctgt ttgacgtact cgttggcatc
    21481 aacctgttgc gcgaaggcct cgacatcccc gaagtctccc tcgtcgccat cctcgatgcc
    21541 gacaaagaag gcttcctgcg ctcccaccgc agcctgattc aaaccatagg ccgcgccgcg
    21601 cgcaacgtga acggcgtcgc catcctgtac gccgacaaaa ttaccgactc catgaaagcc
    21661 gccgtcgacg aaaccgaacg ccgccgcgaa aaacagatta aattcaacga agaacacggt
    21721 atcgtgccgc agcagattaa aaaacaggtc aaagacatca tcgacggcgt gtaccacgaa
    21781 gaagacagtg gcaaaggccg tagacaaggc aaaaacaagg tcaaagtcgg cgaaatccac
    21841 aacgaagaag acgcgattaa agaaatcgcc aaactggaaa aagccatgca gcaggcggct
    21901 agggatttac agtttgaaga agctgctgtg ttgagggata ggattaggaa tattaaagag
    21961 aatttgttgt ttggggcgga gtgattttga aatagtaatc gcagcctgaa tacatagcgt
    22021 gtgcgccact ggcgcgcgct cattgtgtta tcaggaatcc ttgaaatcat gtacgcagct
    22081 tgcatcacac atcctagcta tacataaaca acaaggccgt ctgaattttc agacggcctt
    22141 ttccgtttac aactttacta cttattcaat aactacacgt gcattcagag gctgtacggg
    22201 gcggttgttt tccgaaccga cggcgcgggt gaatttttcc gcttgcgctt ggtcgatgtg
    22261 gtcataagtt ttcaacacca accatgatac gccctctgtg cacggcggcg tggtcagcga
    22321 accggcaaag cggtagtatt tcaaccgttt cggcagtagg gtggatgcgt cgaacggttg
    22381 gttgagtttc acttttcctg cggtcatcgg catgacgttc cagatggaag acaggcggcc
    22441 gttggttttg ccggcttcat acagcacggc taatactaaa ggctgtttgt tttcgtctaa
    22501 gtggacgaag tgagcttcca tcgggaaagt gcggcctttg atttggtttt cgctcggcac
    22561 gtggaagtgg aactgtttca gggtataggt gcggccgttc acggtcaggg tattgccgcc
    22621 ttcgggataa ttgacctgaa tggtgtggcc gttgttttcc acgtcaacca tactcggttt
    22681 gtaattgact ttgatggcgg gcagtttgcc ggaaacggtt tcggtaatgt ttaccggaga
    22741 ttggtttttg ccggtggagc acaaacggaa ttcttctgac agattgcccc agctttcggg
    22801 agagtcgtgt ccggtatagc cccaatgggt gtgattgccg tgtgcggcgg cggagaaagc
    22861 ggtacaggcc aacagcagga cagcggtcag gcggggtaaa gtgcggggga aacgaggcat
    22921 tgtaagatcc tttctattaa ggagtagaaa aaaggttgca gtgtgtatgt aaatatttta
    22981 ttttttatta aatgatatct atattaagtg cttaagtcaa acttcccggt ctgaaaacca
    23041 ttagagcaat gaaattaaag tgaatttatt tttctataag tcctgcttta tttggataac
    23101 catgaggcat ggctaccttt atccggaaaa atattaatag ccgcaacaaa gatatttgaa
    23161 tccgattcaa atatcaccag caagcgtagc tcggatactt gtatccgaca aagcctttaa
    23221 catttccatc gttgcaatcc attgcagcaa ttaccaaaat gtcggattcg agaatccgat
    23281 ctacggaaaa ctaaagattt tatgcgggct acggcttgct tgttgtattt aatcatattt
    23341 atttgaattg aattctttta ttttattgtg agatgaaaat agtattttcg atgagaaacc
    23401 catgactaaa atagtatagt ctttatcatt ctttacgaag taacaattac catatcctgc
    23461 aatattttta tcccaaaaat cacaatatga ttcagtggta aaatcagttt tagttgatat
    23521 aaaatcaaaa ttatttgggt aagatccata ttgctggtaa tatttatcta tattttctac
    23581 taagaaatca atcctttttt ctaacatttt aagattaatt tgcaataata aaatgtataa
    23641 aactatacta attgctatta cttttaaata tgtcatttta ttttccagta aatagaagtt
    23701 tagttgtttt gttaactatg cctgaatgta cacctaagtc ttcttttggt aacgatgaat
    23761 aattagcaag ccgtagcctg catctccgcc aaccgcgtgc cactatctgc gggcggaata
    23821 aactttaaaa ccgatgtgca ggttaaaacc tacaaggatg cgtgcggtat acacgcgttt
    23881 ttgccgttca aaccattgtt tcaaaacttg tattgaaaca tcgcccgctg ctttatcttg
    23941 aaagaatgcc gtctgtaagc aaacatgctt cagacggcat ttttttatcc ggcggtctgt
    24001 tatccgatcc gtttgaatac caagtcccaa acgccgtgtc cgaggcgttt gccgcgcgct
    24061 tcgaatttgg tttcggggcg gtagtccgga gtgggggcgt agtctgccgc cgtattttgc
    24121 aggctgtcga agctgctgag gacttcaagc atctgctgtg catattcttc ccagtctgtc
    24181 gccaggtgga tatagccgcc ggttttgagt ttgggcagta gtttggcgat gaagggggct
    24241 tgtatcagac ggcgtttgtg gtggcgtttt ttgtgccacg ggtcggggaa gaatatgtgg
    24301 atgccgtcga gcgagccgtc ttgcagcata tttttgacaa cttctacggc atcgtgccgc
    24361 atcacgcgga tgttttccaa atggttttcg ttgatgagtt tgagcaggtt gcctacgccg
    24421 gggccgtgta cgtcgatggc gagaaagtcg gtttcgggca ggcggcgggc gatttctgcg
    24481 cttgccattc ccataccgaa gcctatttcg aggattttag gtcggctgct tccgaaacgg
    24541 gcatcaagat cggccggtgt tgcttggtag tcgatgccga agctgtccca taaggtatcg
    24601 atggcgcgtt gctgtgcggc ggtcatgtgg ctttggcgga ggacgaaact gcggatgctg
    24661 cgggggtgtc cggcttgacg gtcgtttggt gtttggtttt cggcggtgtc tgtcataatg
    24721 cttctgtgcg cttgtgcaat caggatgccg tctgaatgtg aaaggcctgt tcggacggcg
    24781 ggaagggaaa ggggcgatta tacctgtttc gtttcggcag ggtaggtgta actctgttca
    24841 gacggcattt tgccactgtt gcagcagttc ttgcaggacg cttttttgcc cgtatccgtt
    24901 gtttatggct tcttgcaggt agcggcaggc tttttctttg tccgcctggc accgtgtccg
    24961 ctgtaatgga tgcaggcgag gttgtagaac gccatactgt ctttttgcgc ggcggcttgt
    25021 tcaaaccatt tgcgggcttt gcagtagtcg gcggctatgc cctgtccgaa gtagtacata
    25081 ctgcccagat tggtttgggc tttgctgtgt ccgagttctg ctgcttctgt gtagagtttg
    25141 agcgcggctg cgtattcggg cgcgcattgt aatccgtaat gcagggcttg ggctttttga
    25201 taaagctgtt ctgccctttg ccgcctaatg gcggaatctt tgatgccgtc atattgctcg
    25261 ggatggttta acgcgctgtc ggatatgagt ttctgatagg cggtaaggct gccggcttcg
    25321 gctgcctgcc ggtaataatg ccgggctttt tccgtatcga gggctatgcc caaaccgtag
    25381 cggcagatgt cgcccggtat ccgcaaacct tcgggatggc gttcggcggc ggctgcttcg
    25441 gcatgatgca gggcaagttt gtggtttctt tccagatact tgccttgcag gtagatgtcg
    25501 gccagtgcgg catgggcatc ggggtcattt tcctgtgccg cctgttggaa ccggtaaacg
    25561 gcttcatcgg gagccgtagg taggatgagt tcggcaagcg gggtgtaggc gggaatgtat
    25621 ccttgttgcg cggcggcgcg caaatgattg acggcctgtg ccgtgtcggc tttcgtccct
    25681 tgtccgaaac ggtattgcag cccgagttgc caatgtgctt ccggcaagcc ttgtgccgcc
    25741 gctttgcggt agagcaggtg cgccgtttca aaatccggcg gcgtgccgtg ccggcagtat
    25801 tgggcgagca ggtattgccc gtggggattg ttgttttttg cgcttgtttt gcccattcca
    25861 gggcttcttc aaaatgtcgg gacgcgtaca gaaggtacat caggcggact tgcgcttcgg
    25921 gcaggcctgc ttctgccaga ggcctgtaaa gcggtatcag ggtttccggg ggcgcgcctt
    25981 ccaattcttg gatatatgcc aaattgtaac gcgcataggg atgcccctgt tcggcggcga
    26041 tgcggagcat ttccatacct tgttcgcggt cttggtgcgg gctgtccgcg ttcatcaggt
    26101 gttttgccaa ctcgaatgtg gcggcgggat gatctgccga ggccgcttct gtcagcagtc
    26161 ggcggacttc ggtaaagtta taatattgtt tttgagacag gcgttccaag gcctgccgat
    26221 agggtaagtc gggattgttc atatcagatc gttttccgtt ttgtcgaatc atcgttattc
    26281 gtcatgaagt taggtttata tcggtatgcc ggcctgctgt atcatatttt tacaggcggt
    26341 ttttgctttg gcggcatact aatgcagatg attataaatg gtttccgcca atgccttgct
    26401 gatgccttcc actttttcca agtcctcgcg gctggcggca atcacgccgc gcagtccgcc
    26461 gaagcgggta agcagggctt ggcggcgttt gctgcctaca ccgggaatgt cgccgagaga
    26521 cgaggtaacg cgtgctttgt cgcgtttttt acggtggcct gtgatggcaa agcggtgtga
    26581 ttcatcgcgt acggtttgca ataaatgcaa ggccgggcta ttaggaggca ggcggaagag
    26641 ttcgccggta aaaggcagta tgagttcttc catgccggct ttgcgttcgg ggcctttggc
    26701 aataccgact aaagggatgt gcagcccgag ttcttcccat acggatacgg ctatgccgat
    26761 ttgcccttta ccgccgtcaa tcaacacgac atccggccat ttgacgcttt cgccgttggc
    26821 ttcggcttcc tgtattttgc cataacggcg cgtcaacact tcgcgcatgg cggcgtagtc
    26881 gtcgccgggt ttggcggtgg tgatgttgta gcggcggtat tgcgagggtt ggatgttttg
    26941 ttcgtcgtac acgacgcagg aggcgatggt ggcttcgcct tgcgtgtggc tgatatcgaa
    27001 acattcaagg cggttgattc cgtctgaatt catgccgagg attttggcaa gttcgtcgat
    27061 gcggtgttgt tggttgcttt gttgcaggtg gcgttgggta atcgccattt gtgcgttttg
    27121 ttccgccatt ttcaaccaaa ctttgcgttc gcctatggtt ttggtaacga actgcatctg
    27181 cttgccgtgt tctccttcca aagcctcttt caacgcatcg ggaacgggaa agttgctgat
    27241 gatgatgtcg ggtttgcttt tgcccagata gtgttgggcg acgaaggctt cggcgtaatc
    27301 ttgaccgttt ggttcggggt cgtttttggt gtcgggaaag aagcttttgt cgccgacgtg
    27361 ccgtccgccg cggatgctga cccagtgtac gcagacaagg ccgtctgaaa cggcgagcgc
    27421 gagcaggtcg atatcgttgg ggttgttcgg gtttttgctg tcgatgaact ggttgctctg
    27481 tataatgccg agtgcttgga tttggtcgcg ataacgggcg gcttcttcaa attgcagatt
    27541 cgcggcggcg gtctgcattt tgtgttgcag ggtgcgggtc agttcgtcgg ttttgccgtt
    27601 gaggaaagtg gcggcttgac gcacgctgtc gcagtagtct tcttcgctga tgtgtccgac
    27661 gcagggcgcg gtgcagcgtt tgatttggta cagtaggcaa gggcggtcgc ggtgttcgaa
    27721 cacgctgtct tcgcaggtac gcagcatgaa gactttttgc aacacttgaa tgctgtcgcg
    27781 cacggcgttg ctgttcggat acgggccgaa gtattggttg ggatttttca gcgtgccgcg
    27841 gtaatacgcc atttgcggat attgatggcc gctgagcatc aaataaggat agcttttgtc
    27901 gtcgcaaaaa aggatattgt atttcggcga caaggctttg atgaagttgt tttcgagaat
    27961 cagggcttcg gcttcggaac gcgtgatggt ggtttcgatg tggcgaacct gtttcaccat
    28021 caacgcgatg cgcggcgaat ggtcgttttt ttggaaatag ccggacacgc gccgcttgag
    28081 gttgaccgct ttgccgacgt ataagacgtt gccgccttcg tcaaaaaaac ggtacacgcc
    28141 cggcagcttg ggcaggtttt tgaggaagag cgggatgtcg aattgttccg gagaacggtt
    28201 ttctgtattc atatcaatca tttgaaaaac ggcttcagac ggcatatcgg cagcggcagc
    28261 cttatccgtt cggaaaagct gtcgggcggt tatatgcggt caatcgattt ttacccattt
    28321 caggcgcagg gcattgccca ataccgaaac cgagcttgcc gccattgccg cgcctgctat
    28381 gacgggattt aaaaagccga gcgcggcgag cggaatgccc aatatattgt agaagaaggc
    28441 gaaaaatagg ttttgcttga tgttttccaa cgttgcctgc gatatcagca gggcatcggc
    28501 gagctgattg accgaatgct gcatcagcgt ggcggaggcg gtgtgttcgg caacgtccgc
    28561 accgcctttc atggcgaagc tgacgttggc ggcggcaagc gcgggcgcgt cgttgatgcc
    28621 gtcgccgacc atcgccacgg ttttgccggc ggctttgagt ttctgcactt cggcggcttt
    28681 gtcgcacgga ctcatattac cgaaggcgtg tgcgatgccc agttgtttgg cgacgtattc
    28741 gaccgtactt tggttatcgc cgctcataat atagacatcg atattgtgtt ttttcagacg
    28801 gcctatggct tcggcggtat cggctttcaa cgcgtcggag agtgcgaatg cgccgatcgg
    28861 tttgccgttt acagatacgg taaccgcact ggcgatttcc caaacgccgt ctgaaaactt
    28921 cggcaaggtc agttcggcaa attcggcttt gcctgatttt accaaaccca cgccttccac
    28981 ttcggcggta atgcctgctc cgacaacggt ttgcgcattt tgtgcagcgg gaatctccaa
    29041 accgcgcgct tgtgcggcgg agacgatggc gcgggcgagc gggtgggcgg cgttttgctc
    29101 gacggcggcg gcgatgcggt acaaagcgtc ttcgtcaaag ccgctgtcgg gaacgtaata
    29161 aacggcggca acctgcggcc tgccttcggt cagcgtaccg gttttgtcca atacgacggc
    29221 atcgacgtgg gctgcttcct ccattgccgc cgcgtcttta aaccaaatgc cgtgtttcac
    29281 cgctttgccc atgccgacca taatcgcggc aggggtcgcc agaccgagcg cgcacgggca
    29341 ggcaatcacc aaaacggcaa cggcgtgcat cagtgcgacc gtccaatcgc ccttaatcag
    29401 ccaagtaacg ataaaagtca gaagcgcgat gcccacgaca gttggcacaa ataccgccgc
    29461 cgctttatcg gccacgcgcg caatcggtgc tttactgcct tgtgcttcag agagcgcgtt
    29521 catcatgtcg ccgagcaggg tttggctgcc gagctgcgcg gcgcggtaca ccacgctgcc
    29581 ttcggtcatc agcgcgcccg ccaacacttt gccgcccgcc tttttctctt cgggattgga
    29641 ttcgccggta aggtggcttt cgtccgccca accgctgccg ctttcgataa tgccgtcggc
    29701 agcgatgcgt tcgccgtggt tggtgcggat aaggtcgccg atttgcactt ggtcgatggg
    29761 cagttgtttc cattcgccgt tgcgttgcac gttgacttgg gtcggcgtga gttttagcag
    29821 taagcccaag ctgttcaggc tggatttttt ggtgcggtgt tccaaaaact tacccagcga
    29881 cacaaaaccg atcaccatca cgcccgcttc aaaatacaca tgcgccatac cgtgcgccgc
    29941 atgcgaactg aaaaacagca tataaacgga atacaggtaa atcgacaccg tgccgatggt
    30001 aacgagtacg tccatattcg ccagcccgcc tttaatgctt gcccacgcgc ttttgtaaaa
    30061 cgggattgcc agccaaagtt gcactatgct tgccagtaca aactgccata caggcggaat
    30121 catccaatcg tgccgtgtcc aattcagccc ttttagcatc atccctacca taccgataag
    30181 gaacgggata ttgatggtca gcaaaagcca caaccgccag ccgatatggt gttctgcttc
    30241 aggttgcggc aatgtatctt ccgttttttc cttcgcgccg taaccggttt tctcaatgat
    30301 tttggcaatg tcggcaaccg aggttttgct gccgtcaaac gtaacctgcg cttcctcact
    30361 ggcaaagttc actcccgccg attcgacaaa atcttttttg ttcaacactt tttcaatgcg
    30421 cgaagcacat gcctgacagg tcatcccctc gatttggaaa cggatttttt gttgcataag
    30481 tttgattccc gttttgatgt tcagacggca gtcaaggctg cgggatgccg tctgaacgcc
    30541 ggtatggtca aaatttctca aacgcgccga taattcgggt agaatccgaa aacttgacac
    30601 agcaaaggaa aaacaatgtc ccaacaccgc aaactgatta ttttgggttc cggccccgcc
    30661 ggatacaccg ccgccgtcta tgccgcacgt gccaatttaa accccgtcat tattacaggt
    30721 atcgcgcaag gcgggcaact gatgacaacg actgaagtgg acaactggcc tgccgatgcc
    30781 gacggcgtgc aagggccgga attgatggcg cggtttctcg cccacgccga acgtttcgga
    30841 acggaaatca tttttgacca aatcaacgcc gtcgatctgc aaaaacgccc gttcgcactc
    30901 aaaggcgata tgggcgagta cacttgcgat gccctgattg tcgccaccgg cgcgtccgcc
    30961 aaataccttg gtttgccgag tgaggaagcg tttgcaggga aaggcgtttc cgcctgcgcc
    31021 acctgcgacg gtttcttcta caaaaaccaa gatgttgccg tagtcggcgg cggcaatacg
    31081 gcagttgagg aggcactcta ccttgccaat atcgccaaaa ccgttacgct gattcaccgc
    31141 cgcagcgagt tccgtgctga aaaaatcatg attgacaaac tgatgaaacg cgtggaagag
    31201 ggcaaaatca tcctcaagct ggaaagcaac ctgcaagaag tactgggcga cgatcggggc
    31261 gtaaatggcg cattattaaa aaacaacgac ggttctgatc aacaaattgc cgtcagcggc
    31321 atctttatcg ccatcgggca caagccgaat accgatattt tcaaagggca gttggaaatg
    31381 gacgaagccg gctacctgaa aacaaaaggc ggcacggcgg acaatgtcgg tgcaaccaat
    31441 atcgaaggcg tatgggcggc gggcgacgta aaagaccata cctaccgtca ggcaattacc
    31501 agcgcggcct ccggctgtca ggcggctttg gacgcggaac gttggctggg cagccaaaac
    31561 atttgaatgc cgtctgaaac cgaaattttc ggcggaatgc aagtttttcg taccaaatcc
    31621 ttgattccgt cggcaaaccc caatataatg cctgctattt gaaccttgcc ttctgctttc
    31681 gcatggcaga aggctgtttt taccatccat aaggagataa catgcgtcat tacgagatcg
    31741 tgtttatcgt tcatcctgat caaagcgagc aagtgcccgc tatggttgaa cgttacaaaa
    31801 ccatgattac cgaggcaaac ggtaagattc accgtttgga agattggggc cgccgccaac
    31861 tggcttaccc gattaacaaa atccataaag cacattatgt tttgatgaac atcgaaacca
    31921 ctcccgaagt tgttggggag ttggaaaccg cgttccgttt caatgatgcg gtattgcgtc
    31981 atctgaccat caaaaccaaa cacgccgtta ccgaagcctc ccctatgctg ggcggcgaaa
    32041 aggctaagaa cctgttggtc ggcgcggctg aagaagcggc tgcccaataa ttgggattca
    32101 ctaatcttgt ttcgcttgcc gcgctgattg aaaaggcttt ccctattcga tatacgcctg
    32161 ccggaatccc tgttttagat attattttaa agcacgaatc gtggcaggag gaaaatgggc
    32221 agcaatgcct tgtccaattg gaaatccccg cgcggatttt gggtaggcag gcggaagagt
    32281 ggcagtatcg gcaaggtgta tatgttcacg tcgaaggttt tttagctcaa aaaagcagac
    32341 gttccctgat gccgatgctc aggatacaaa atattaaaga atataaaggt taaacgacaa
    32401 tggctcgtca atcattcaaa cgtagaaaat tctgccgttt cacggctgaa aaaatccaag
    32461 aagtcgatta caaacaagtt gatttgctga aagactttat ctctgaaaac ggtaaaatca
    32521 ttcctgcccg catcacagga acgaaggcat tctaccaacg ccaattggct gttgccgtaa
    32581 aacgcgcgcg cttcttggca ctcctgcctt ataccgacca acacaaataa ttttggagtt
    32641 taaatcatgc aaattattct gttagaaaaa atcggcggtt tgggtaattt gggcgacatc
    32701 gttaccgtaa aaaacggtta tgcccgcaac ttcttgattc ctgctggtaa ggcaaaacgt
    32761 gctactgaag caaacatgaa agagtttgaa gcacgccgcg ccgaattgga agccaaacag
    32821 gccgaaattt tggcagatgc ccgagcccgt caggaaaaac tggacggtca aaccgttacc
    32881 gttgcacaga aagccggtgt ggacggccgc ctgttcggtt ccgttaccaa tgccgacatt
    32941 gctgctgcaa tcgttgctgc cggcatcgaa gccgtgaaag caaatgtacg tctgccgaac
    33001 ggtcctctga aagccgttgg agagtacgaa gtggaagtgg ctttacacac tgatgccgtt
    33061 gctaaaatta ctgttgccgt cattgccgca gccgagtaat tgaaattatc agatgccgtc
    33121 tgaaaccctg ccgattatca gaatgtggtt tcagacggca tttgttttgt tttccgtata
    33181 ttctgcaaaa atcgggacgg ttgtttttta tttgcctgag gtctttgcag aaatggtttg
    33241 ttaacgaaat ttgatgcata aaatgcgcca aaaaatattg gctgtgtttt aatatagtgt
    33301 tgacaagcgt acctggtttc aatatattca ataggatagg tctcttccca accgaaaaaa
    33361 taattgtctt ttctcttgta aatagttgaa atagattgtc aaaatacatc ctaaaaaatt
    33421 taataactta tatgattgca aaggttgcgg cttgtatgcc ggtcattatg gcggaaatac
    33481 cgtttgggag gcagtcgcat cgcatggagt cggcacggca ggcgatgcgc cgatgccttc
    33541 gggcgtgaag gcagggaaat cggcagtttg gaaaaaggca atggaatccg caggtaggtg
    33601 tcgattgggt ggtgaaaggc aataagtcgg gcgcacgctc agactgccta tccccttgtc
    33661 ggaacgggag gtagggcagc tttgtcctga ctaattgctt gtgcaaacgg taagaaggtt
    33721 tttaaagcag gttgtacagc atttttccgg caatcaaaag caacataatg ccgaaggatt
    33781 ctttgagttt ggcagaagaa agtttgtggg cggttttgac accgagcggg gcaaaggcaa
    33841 tagttgccgc gctgaggacg gcgacggcgg gcaggtaaag gaagcccagc gacccttcgg
    33901 gcaatcctgc aatattcaga ccgttgacca gatacgatat tgcgccggag agtgcaatcg
    33961 gccaggcaag gccggatgat gtgccgatgg ctttatgggc ggggaagccg cagtggatta
    34021 agaaggggac ggaaagtgaa ccgccgccta tgccgaccca gctcgacatt gcgccgaaca
    34081 gtgtggaaac cgcagtcagt ccgggcagcc cgggcagcgg gcgggatgcc gtctgacgac
    34141 cggtatgcag tgttttgaat gcgacggcgg ttaaaaacag gatgaagaaa atttgaagcc
    34201 cgaacgcggg gatatatttt gcggagagtg cgccagcgaa tacgccgaat atcatacccg
    34261 gcatcatcgc aaatatggtt ttccagtcga ccgtctgttt tttgtgctgc cccaacatac
    34321 tggaaaaggc ggtgaagacc atgacggcga aggatgtgcc gacggcgagg tgttgcgcgt
    34381 aaggatgttg tgccaaaccc tgcaaatcaa gcacccataa aacgacaggg acaatcagcg
    34441 taccgccgcc tacaccgaac aggccggcaa taaaacctgc cgcactgcct acggcaagca
    34501 ggattaagat aatgtcccaa tgccacattt aatccgcctt tctgtatttt ttccaaaatc
    34561 ggcgtgccgc cataatgtag ccggacaggc tgtatccgag aaagaacagg aaaaggacga
    34621 gcgacggttc ccaagtgacc agaagcagca gtaagacggc aagcagcatt ccgacaaagg
    34681 ggacttgtct gcggatgttg atttctttaa aactccaaaa agggatttgg acaatcatcg
    34741 acaggccggc aaacagtgtg atgcctaatg cccaccagtg gacggcgggg aatttttcga
    34801 cgctgtggtt gacccaaatc agcccgacaa tcagcgcggc ggcagtcgga ctgggtacgc
    34861 cgataaacca gcgtttgtcc accttgccga tgagcgtgtt gaacagcgcg aggcgtaggg
    34921 cggcgcaggc gcagtagatg aaggcgacgg aataaccgat tttgccgaac tgccaaagct
    34981 gccatttgta ggcaataagc gcgggggcaa cgccgaagct gaccatgtcg gcaaggctgt
    35041 cgagctgctc cccgaacgcg ctttggctgt tggtcagccg cgccacgcgc ccgtccatac
    35101 cgtccagcag catagagatg aataccgcga tggcggcggt ttcataacgt ccgtgcatgg
    35161 attgggtaat ggcgtaaaac gcggaaaaca gcgcggcgat ggtaaaggaa ttgggcagca
    35221 ggtagatact gttttgacgc agggagcgtt gcggggtgtt ggtcggggtt tccatagctg
    35281 aagtgtctca cggacgtgga gaatcgatag gggattataa cccaaaccat tatcgaacaa
    35341 tacgcccttt aggatgaagc cctcggaggc ggtttgtttt tctgatggcg gatgctttgc
    35401 ggggcggttt gactctgcct gccaccgggt gtgttgtttc ggattttgcc ggatggatat
    35461 tgttgccggg catttctgcg cttgccggaa ggtatcggag ggaaggcatc ggaatatgcg
    35521 gcgcgccgtt caaaccgggg cggtatttcc ctattttttg gggggtgcag gtttcaacaa
    35581 attgaaagat attttttgca tttccgaccg tatgctgatt ttgaagcggg ataaattata
    35641 ggacaaggtt tttcgcccgc cgcaaaggtt gcccgcaatg ccgtctgaaa acaacggctt
    35701 gccgctaagg cggcaatatg aggatttgcg ctgccgaaac cggtttgcta caacccggca
    35761 gtgaaatctg cagggaggga taggccgata aggcgggact tatcttgttt tttgtttata
    35821 atgcaacaga atttggagga atcgtcggga atttatcatg atgaaaaaga tactggcagt
    35881 atcggcacta tgcctgatga ctgcggcggc acaggctgcc gatacttacg gctatctcgc
    35941 cgtttggcag aatccgcagg atgcaaacga tgttttgcag gttaaaacca caaaagaaga
    36001 ttcggcgaaa agcgaagcgt ttgccgagtt ggaagccttt tgcaaaggtc aggacacgct
    36061 tgcgggcatt gccgaagacg agccgaccgg atgccggtcg gtcgtgtcgc tgaacaatac
    36121 ctgtgtctcg ctggcatacc cgaaagcctt gggcgcgatg cgcgttgaaa acgccgtcgt
    36181 gattacttct ccgcgtttta cgagcgttca tcaggtcgca ctcaaccagt gcataaaaaa
    36241 atacggcgca cagggacaat gcggcttgga aacagtgtat tgcacgtcat cttcttatta
    36301 cggcggggct gttcgctcct taatccaaca cctgaaataa aaaatgccgt ctgaaggatg
    36361 ttcggacggc atttccatat cgacggtcag gattctttcg gatcgggcag caggctgttc
    36421 aacacaatgg cgagtacggc gcacaagccc acgccgacaa agctgaagct gcccaatttg
    36481 agtgtcatac cgccgatgcc cgtggtcagt accgagctga cgatgaccag gtttttcggc
    36541 agcatcaaat cgactttggc atcaatcagg gttttgatgc ctaaagaagc aatcgtgccg
    36601 aacagcagca gcataatgcc gcccatcacg ggcatcggaa tggaagccaa aaacgcattg
    36661 aatttgccga aaaacgccat gcagacggca aaaaccgccg cccaagtcat gatgacgggg
    36721 ttgctgtttt tggtaatcat caccgcaccc gttacttcgc cgtaggttgt aaccggcggg
    36781 ccgccgatca gacctgcaac gcacacgccc aagccgtcgc ccgcaagggt tttgtccaag
    36841 cccgggtctt ttgtgtagtc ttttcccgtt acattgccga ttgccatgat gccgccgatg
    36901 tgttcgatgg cgggggcgac ggcaacgggc agcataaaca gtgccgcctg ccagttgacc
    36961 tgaggcgttt caaaatgggg aacggcgaac cagggcgcgt gtgcaatgct tgtcgtgtcc
    37021 accagtccca tcagcagtgc caaaacataa cccgaagcga caccgatcaa gatgggaatc
    37081 agcttcatca tcctgctgcc gaaaaccgat acgatggcgg taacggcaaa ggtaaagccg
    37141 gaaagaatca gcgaatcggt atagtcgatg acctgtttgc cgtcagcctg acccattgcc
    37201 atgctgcttg ccgccacggc aacagacaga ccgatgacca tgatgacagg gccggtgacg
    37261 accggcggca gcagtttgtg taccgccgcc agtccgcgcc aacggatcag cgcggcaaac
    37321 acaaaataca taaagccggc ggcaaacagc ccgaacatgg tggaaggcag cccccattcg
    37381 ccgacggagt agataatcgg cgcgataaag gcaaacgaag aaccgagaaa aatcggtact
    37441 ttgcgtttgg ttgtgatttg gaacagcagc gttcccaagc ctgcgcccaa aagcgcaaga
    37501 gccggattca gaccggtcag caggggaacc agcaccattg cgccgaatgc cacaaataaa
    37561 atctgcgcgc cggaaacggc aagtttcagt tggttcatac catcttcttt caatcggttc
    37621 aaacggcacg gattatactg gtttgccgag gggctgcgta aaaatacata cgtcgggcaa
    37681 agatatgccg tctgaaaggc aacgtctgtc tgttgcagga tgaaagcggg agaaatggag
    37741 tataatccga cactttccat attcaatccg caagccgtcc cgaaccctta tttcagacgg
    37801 cattttccgg taatacggca tgacagaaaa atcccataaa aaaacagcaa aaggcagggc
    37861 gggaagcccg tccccaacgt ccgcacgcaa caagaaagcc gacaacggcg cgcggggcaa
    37921 taaagtttcc gagcggctca aggcggtcaa agagctgcag aaaactgaag ccaaaaaggc
    37981 gcgtcccgaa catgtcgtca acctgattgc cgacgcgctg tggctgatgg gtttggcggc
    38041 aaccctgtat ttggcgattt ccctgatcag tttcgatatg ggcgatccgt cttggtcgca
    38101 cagttcaccg gttgtggaag atgccgccaa ttggggcgga ctgttcggcg cgtatgttgc
    38161 cgatgtcggc tattatcttt tcggctggtc gttctggtgg tggatagcgg ctgcctgcgt
    38221 cgtgctgtat aaaaatttcc gtctgcacgc aaaacagacg gaaaacgagg catacaacca
    38281 caaaatcgct gccgccgcgc tgtttgtcct gacggtcttc agccccgtct tggagtattt
    38341 tgtgctgggc ggaaaatatg ccgactccct gcctgtcgga gcaggcggta tggtcggcat
    38401 acgtgtcggc gcagtgtttg cgtggctgtt ggggaaatcg ggcagcctgc tgattatcct
    38461 ggttgttctg ctgttgtcgt tgtccctgct ggtgcagatt tcatggctgg aatttttgaa
    38521 cggtgcgggc agggcggttc aaaaccgcct gagtgcctta tccggcaagg tcatggcttt
    38581 gagaaaacgc cggccgaata ccaaaacaga cggtgtcgat acccaaaata cacggcgcat
    38641 ggtgaaagaa gccaaaaaca tcactgccaa acccgttgcc ttgcccgaag gcagcagcag
    38701 caaccgcaaa tccgtcgcgg tttccgttgc gccgccgcct aaaatccaac cttcgctgtt
    38761 tgaagacaat gaagtacagc agaacggcga ataccacaag cctacattga acctattgcg
    38821 gattcccgac agcgagccgg tcagcatcaa tcccgccgaa ttggagcgca ctgccgaact
    38881 aatcgaatcc aaactggcag aattcggcat cggcgtacaa gtcgtatccg cgacatccgg
    38941 ccccgtcatc acgcgctacg aaatcgaacc cgcgcaaggt gtcaaaggca gccaaattgt
    39001 tgccctgtcg aaagatttgg cacgctctat gtcgctgcaa tccgtgcgta tcgtcgaaac
    39061 cattgcgggc aaaaacacga tgggcatcga gttgcccaac gacaaacgcc aagacgtgat
    39121 gttgagcgaa atcttgtcct cgcccgtgtt tgccgaagcc aaatccaagc tgaccgtcgc
    39181 gctgggcaaa gacatcgccg gtacgcccgt cgtcggcgac ttggcgaaaa tgccgcacct
    39241 tttggtcgcc ggcatgaccg gttcgggcaa atccgtcggc gtgaacggca tgattatgtc
    39301 tatgcttttc aaagctacgc ccgaagaagt ccgcttcatt atgatagacc cgaaaatgct
    39361 cgagttgagc atttacgacg gcattccgca cctactctgt cccgtcgtta ccgatatgcg
    39421 cgaagcaggg caggcgttga actggtgcgt cgccgaaatg gaaaaacgct accgcctgct
    39481 ctcccacgcc ggtgtacgca atctggaagg cttcaaccaa aaagtcgaac aagccaaagc
    39541 ggcaggcaag ccgctgctca atccgttcag cctgaacctc gacgaacctg agccactgga
    39601 aaaactgccg atgatcgtgg tcgttatcga cgaacttgcc gacctgatga tgaccgaacg
    39661 caaagctgtc gagcagcaaa tcgcccgcct cgcccaaaaa gcgcgcgccg ccggcataca
    39721 catgattgtc gccacccaac gcccaagcgt cgatgtcgta accggtctga ttaaagccaa
    39781 catcccgacg cgtatggcgt ttaccgtgca aagcaaaatc gacagccgta ccatcctcga
    39841 ccaaatgggc gcggacgaac tgctcaaata tggcgattcg ctgttcctgc aaccgggcag
    39901 tgccgaaccg acccgcctgc aaggcacgtt tgtttcagac ggcgaagtgc atcaagtcgt
    39961 caactacgtc aaatcgcaag ccccagccga ctatattgaa ggtctgctca gcggcgaagc
    40021 cgcgctggaa actgccaata tcgttaatcc gaatgcagac agcgacgaat tattcgatca
    40081 ggcagtcgcc tatgttttgg aaagcaaaaa aacctccatt tcgtctttgc agcggcagtt
    40141 gcgtatcggc tataaccgcg cggcaaacct gatggaggca ctggaaaatg cgggtatcgt
    40201 ttctccctcc gacctcaacg gcagccgtaa aattttggcg cacaaggacc atttatagcc
    40261 cgtatcgc
//
LOCUS       DAWTQS010000011        38955 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.011, whole genome shotgun sequence.
ACCESSION   DAWTQS010000011 DAWTQS010000000
VERSION     DAWTQS010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 38955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38955
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.011"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            194..2200
                     /locus_tag="WNA25_000585"
     CDS             194..2200
                     /locus_tag="WNA25_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HFA9823406.1"
                     /translation="MADMKKITPQNLRPLLSESLGHTDFVNVLNALIKFLRRGGKKCA
                     GERFDLIIDTFKQDRELLSRFSRCFYIWLAQIHIYPALIKLGIFSRHSFAREMGIRIY
                     ERFNPSYKDFANLGEVFLYLFHSENDDKWLQTLNIRQWLVLYELIRSHAEPSKLQTAG
                     IRLADARLRAIEMLSVWTASEAIEPDLIRIAPRLLEADSSFVALQRETAKLVEHYRNG
                     TAPYDTAHLEVMFDQCFSQIDYLRRKGTGAGSGSSVKVAHLLERLRQTVGRLKLLTDI
                     QTGAGNSNRLTIALMNSLIYAAVEQYSTRHLRRSSIRMLARSITENKSHHGEHYITRN
                     RKEYFKMFYSAAGGGGIIALMALLKIRIGTLDLSPFLASLSAGFNYGIGFMIIHMLHC
                     TVATKQPAMTAASFAEQVDLNEGGKAVDNKLSKLLIDVCRSQSVAVFGNVSIAILLAC
                     AISFGYAHLYRLPILDAHTAAYQFKSIDIINHPTLWYAAIAGLWLFCSGIIAGFFDNR
                     ADYLNLRQRLPFNPLLRKIMRPGPRRVLAAYIHKHYGSLVGNFIFGMLLGMTGYFGHL
                     LGLPLDIRHVAFSSANLGYAAVSGNVGFGTFVLGIFSVLAIGLVNLCVSFSLALFVAL
                     RSRGTKIGSIRNLIKSFWNQIKSNPCILFLPPAKGQGHPPSDKP"
     gene            complement(2323..3414)
                     /gene="mltB"
                     /locus_tag="WNA25_000586"
     CDS             complement(2323..3414)
                     /gene="mltB"
                     /locus_tag="WNA25_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFA9823407.1"
                     /translation="MEKRKILPLAICLAALSACTAMEARTPRANEAQAPRADEMKKES
                     RPAFDAAAVPVSDSGFAANANVRRFVDDEVGKGDFSQAEWQDFFDKAAYKADIVKIMH
                     RPSTSRPWYVFRTGNSGRAKFHGARRFYAENRAVIDDVAQKYGVPAELIVAIIGIETN
                     YGKNTGSFRVADALATLGFDYPRRAGFFQKELVELLKLAKEEGGDVFAFKGSYAGAMG
                     MPQFMPSSYRKWAVDYDGDGHRDIWGNVGDVAASVANYMKQHGWRTGGKMLVSATLAP
                     GADVQAIIGEKTALTRTVADLKAYGIIPGETLADDEKAVLFKLETAPGVFEYYLGLNN
                     FYTVWQYNHSRMYVTAVRDIANSLGGPGL"
     gene            complement(3562..4989)
                     /locus_tag="WNA25_000587"
     CDS             complement(3562..4989)
                     /locus_tag="WNA25_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HFA9823408.1"
                     /translation="MIHTEPSAQPSTMDTAAFLKHIESAFRRIFSDGIDLMRYLPEGK
                     WLALKQAGLLLPFLDKKHGGRKGSQFEIQEVLRIAGHYGVPVTLRTGIEGALVLQPLQ
                     EFGGEAQVAQGLDMIFKGEGGGLGVTEPETSGAAIAREMQSCYEYTDEQTIYVNAAKY
                     WQGNSQSDFLLVAAKERKNGKLAKVIDLLLVPKTYIRCETLASEGLRAVRYAVNRIDA
                     EMPATAVMKLSRGDAAGLRAFQNIFIRSRLQLIGMTHGIMEYILDNLNRYVRNDIRFV
                     DYERREIQRRHQVSEILYRYVCHSVSPVAPVAHQLMEANIVKTLATEYTYAAAQMLQK
                     LLGAKGFERGHPAGNIAIDIRPFTIFEGPNDMLYAEIYDQFVRATAEEKEAGIKLDKN
                     QTLLDRLQTDVRFAAVARDYALPEDIRSFLQEHTLTDACALQKVFIGKIIARLFVFVQ
                     EEHEDTTAFLLNDIRKDILDCRYCG"
     gene            5174..8878
                     /gene="mfd"
                     /locus_tag="WNA25_000588"
     CDS             5174..8878
                     /gene="mfd"
                     /locus_tag="WNA25_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFA9823409.1"
                     /translation="MTCPIPKPREKSRWFNLSQGSLPLALARYLPHKRLKAVLTQDAE
                     QALRLQTAWRFFRPHDTAVFLPDWETLPYERFSPHQDLVSERLSALWQIKSGAADVLF
                     VPVATAMQKLPPVPFLAGRTFWLKTGQTLDIGRLKTDLVDAGYNHVSHVVAAGEFAVR
                     GGIVDLFPMGSETPYRIDLFDDEIDSIKTFDTDTQRTISPVSEIRLLPAHEFPTDSEA
                     QKIFRSRFREEVDGNPNDAAVYKAVSNGHFGAGVEYYLPLFFENELETLFDYIGEDAL
                     FVSLGDVHAEANRFWNDVKSRYAMAQGDETYPPLLPQHLYLSADVFAGRLKNYGQVLP
                     DVSGKAHSLPDLAVNRQSDEPLQALKDFQTAFDGRILLCAESLGRRETMLGFLQQNGL
                     KAKPVSDWQGFLSAHEPLMITVAPLAYGFKLGGLQSSSQQQTVPASEGEGKAVTDQTE
                     FSASATNPLPSPLPQEREQSAAAVSDGLKAAAVSTESSLYLVASDLHGQTRQQSAPSP
                     VGEGWGEGKAVAAQSAIAVITESDLYQYVARSRVHNRRKKHAAVSDGLLRDLAEINIG
                     DPVVHEEHGIGRYTGLVTMDLGGETNEMMLLEYAGEAQLYVPVSQLHLISRYSGQAHE
                     SVALHKLGSGAWNKAKRKAAEKARDTAAELLNLYAQRAAQSGHKFEINESDYQAFADG
                     FGYEETEDQAAAIAAVIKDLTQAKPMDRLVCGDVGFGKTEVALRAAFVAVMGGKQVAV
                     LAPTTLLVEQHAQNFADRFADFPVKVASLSRFNNSKATKATLEGMADGTVDIVIGTHK
                     LVQDDIKFKNLGLLIIDEEHRFGVRQKEQLKRLRANVDILTMTATPIPRTLSMALEGL
                     RDFSLITTAPSRRLAVKTFVKPFSEGSVREAVLRELKRGGQVFFLHNEVDTIENMRER
                     LETLLPEARIGVAHGQLRERELEQVMRDFLQQRFNVLLCSTIIETGIDIPNANTIIIN
                     RADKFGLAQLHQLRGRVGRSHHQAYAYLLTPEYITKDAEKRLDAIAAADELGAGFTLA
                     MQDLEIRGAGEILGEGQSGEMMQVGFTLYTEMLKQAVRDLKKGRQPDLDAPLGITTEI
                     KLHSPALLPEDYCPDIHERLVLYKRLAVCETVQQINAIHEELVDRFGLPEQPVKTLIE
                     SHHLRLAAKELGIDAIDATSEAVTVTFGKHHCIDPTGIILLIQTDKKYRLAGADKLRF
                     AAEMENIEVRINTVKTVLKTLQGKRLPKGN"
     gene            9104..9406
                     /locus_tag="WNA25_000589"
     CDS             9104..9406
                     /locus_tag="WNA25_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2004 domain-containing protein"
                     /protein_id="HFA9823410.1"
                     /translation="MDAFAAFLSELEKADDAARAALTEYLKRDSRYIDFHTEDTQTSR
                     DAAKFVRTMQLIYISLWAKNPAFAVMDYMPANMESDEILAVKLHLDGSIFSIDWES"
     gene            9568..9951
                     /locus_tag="WNA25_000590"
     CDS             9568..9951
                     /locus_tag="WNA25_000590"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFA9823411.1"
                     /translation="MFRTILGGKIHRATVTEADLNYVGSITVDQDLLDAAGICPNEKV
                     AIVNNNNGERFETYTIAGKRGSGVICLNGAAARLVQKGDIVIIMSYIQLSEPEIAAHE
                     PKVVLVDGNNKIRDIISYEPPHTVL"
     gene            9973..10815
                     /gene="kdsA"
                     /locus_tag="WNA25_000591"
     CDS             9973..10815
                     /gene="kdsA"
                     /locus_tag="WNA25_000591"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFA9823412.1"
                     /translation="MDIKINDITLGNNSPFVLFGGINVLEDLDSTLQTCAHYVEVTRK
                     LGIPYIFKASFDKANRSSIHSYRGVGLEEGLKIFEKVKAEFGIPVITDVHEPHQCQPV
                     AEVCDVIQLPAFLARQTDLVAAMAETGNVINIKKPQFLSPSQMKNIVEKFREAGNGKL
                     ILCERGSSFGYDNLVVDMLGFGVMKQTCGNLPVIFDVTHSLQTRDAGSAASGGRRAQA
                     LDLALAGMATRLAGLFLESHPDPKLAKCDGPSALPLHLLENFLIRIKALDDLIKSQPI
                     LTIE"
     gene            10830..11270
                     /locus_tag="WNA25_000592"
     CDS             10830..11270
                     /locus_tag="WNA25_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HFA9823413.1"
                     /translation="MMQTFRKISLYAATLWLGMQIMAGYIAAPVLFKMLPKMQAGEIA
                     GVLFDILSWSGLAVWGTVLAAAFAALTRRQTALLLFLLSALAANQFLVTPVIEALKYG
                     HENWLLSVAGGSFGMWHGISSMTFMATALLSAVLSWRLSGKEAV"
     gene            11317..12603
                     /gene="eno"
                     /locus_tag="WNA25_000593"
     CDS             11317..12603
                     /gene="eno"
                     /locus_tag="WNA25_000593"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFA9823414.1"
                     /translation="MSAIVDIFAREILDSRGNPTVECDVLLESGVMGRAAVPSGASTG
                     QKEALELRDGDKSRYSGKGVLKAVEHVNNQIAQALIGIDANEQSYIDQIMIELDGTEN
                     KGNLGANATLAVSMAVARAAAEDSGLPLYRYLGGAGPMSLPVPMMNVINGGEHANNSL
                     NIQEFMIMPVGAKSFREALRCGAEIFHALKKLCDSKGFPTTVGDEGGFAPNLNSHKEA
                     LQLMVEAAEAAGYKAGEDVLFALDCASSEFYKDGKYHLEAEGRSYTNAEFAEYLEGLV
                     NEFPIISIEDGMDENDWEGWKLLTEKLGKKVQLVGDDLFVTNPKILAEGIEKGVANAL
                     LVKVNQIGTLSETLKAVDLAKCNRYASVMSHRSGETEDSTIADLAVATNCMQIKTGSL
                     SRSDRMAKYNQLLRIEEELAEAAYYPGKAAFYQLGK"
     gene            12771..13049
                     /gene="ftsB"
                     /locus_tag="WNA25_000594"
     CDS             12771..13049
                     /gene="ftsB"
                     /locus_tag="WNA25_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFA9823415.1"
                     /translation="MKWVTVVLSFALVCCQYSLWFGKGSIGRNSSLREQIAVQEEKNQ
                     TLALRNHSLAAEVYDLENGQEAISEIARVELGYIQDGETFYRLIRHNR"
     gene            complement(13090..13380)
                     /locus_tag="WNA25_000595"
     CDS             complement(13090..13380)
                     /locus_tag="WNA25_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S ferredoxin-like protein"
                     /protein_id="HFA9823416.1"
                     /translation="MARIGTNKGLFELLEGETLLEGLERTGHMVEYQCRSGYCGSCRV
                     KILEGSVTYREPPLAFLGRDEILPCCCCVEGDVRLDCGLAGEDEGLSDSLLK"
     gene            complement(13501..14634)
                     /gene="nrdB"
                     /locus_tag="WNA25_000596"
     CDS             complement(13501..14634)
                     /gene="nrdB"
                     /locus_tag="WNA25_000596"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HFA9823417.1"
                     /translation="MSYSTFPKTKNDALKEPMFFGQPVNVARYDQQKYEVFEKLIEKQ
                     LSFFWRPEEIDVSRDRIDYANLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSI
                     PELETWVETWSFSETIHSRSYTHIIRNIVNDPSVVFDDIVENEYITARAEDIACYYDD
                     LIEYTQYYNLLGEGVHNVGGKPVTVSLRGLKKKLYLCLMCVNVLEAIRFYVSFACSFA
                     FAERELMEGNAKIIKLIARDEALHLTGTQHMLNLMRSGVDDPEMAEIAAELQDECFQL
                     FKKAAEQEKEWAAYLFKDGSMIGLNKEILSQYVEYITNLRMQAVGLSAGFEGANQNPI
                     PWINAWLSSDNVQVAPQEVEISSYLIGQIDSEVNTDDLGDFEL"
     gene            complement(14947..17226)
                     /gene="nrdA"
                     /locus_tag="WNA25_000597"
     CDS             complement(14947..17226)
                     /gene="nrdA"
                     /locus_tag="WNA25_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HFA9823418.1"
                     /translation="MNTPTDLKVTKRDGRLEAIDLDKIHRVVTWAAEGLENVSVSQVE
                     LKSHIQFYNGIRTDDIHETIIKAAADLISEDTPDYQYLAARLAIFHLRKIAYGEYEPP
                     HLYNHVKKLTDAGKYDRHILEDYSREEFDELNAYIDHERDMSFSYAAVKQLEGKYLVQ
                     NRVTRQIYETPQFLYVLVAMCLFSKYPKEVRLGYVKRFYDAVSTFKVSLPTPIMSGVR
                     TPTRQFSSCVLIECDDSLDSINATTSAIVKYVSQRAGIGINAGRIRGLDSEIRGGEAR
                     HTGCIPFFKMFQAAVKSCSQGGVRGGAATLFYPLWHIEAESLLVLKNNRGVEDNRIRQ
                     LDYGVQINRLLYTRLIKGGNITLFSPNEVSGLYEAFFADQDEFERLYTKYEQDPNIRK
                     RIIPAADLFSTLMQERAGTGRIYIQNVDHCNTHSPFDPRVAPVHQSNLCMEIALPTKP
                     LDNINDPDGEIALCTLSAFNLGALNSLDELEGLADLTVRALDALLDYQGYPVEAARTS
                     TMDRRSLGIGVINYAYYLAKNGVRYSDGSALGLTHRTFEAIQYYLLKASANLAKEYGA
                     CTLFNQTVYSQGKLPIDTYKKDLDAVCGEPLHYDWESLRADIVKYGLRNSTLTALMPS
                     ETSSQIANATNGIEPPRGLVTVKASKDGILKQVVPEFETLKNAYETLWQLPGNEGYLK
                     LVGVMQKFVDQAISANTAYDPGKFEGNKVSMKQMLKDLLTAYKYGVKTLYYHNTRDGA
                     DDTQTDIQDDGCAGGACKI"
     gene            17757..18068
                     /locus_tag="WNA25_000598"
     CDS             17757..18068
                     /locus_tag="WNA25_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein"
                     /protein_id="HFA9823419.1"
                     /translation="MLAILLSAILGLVSTTAAAGTSEPAHRHTKHISKANKQMLHPEC
                     RKYLERRAAWYRSQGNVQELRENKKARKAFRTLPYAEQKIQCRAAYEAFDDFDGGRFR
                     R"
     gene            complement(18118..18228)
                     /locus_tag="WNA25_000599"
                     /pseudo
     CDS             complement(18118..18228)
                     /locus_tag="WNA25_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(18331..19098)
                     /locus_tag="WNA25_000600"
     CDS             complement(18331..19098)
                     /locus_tag="WNA25_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HFA9823420.1"
                     /translation="MSSNKASFFTRLRRLCRLTVWLFKTGKNLRGIDGGCPKSRNRAV
                     IALGKGALAALDIGLEVGRPAPEHPNGVLVAANHVSWLDIFAMSAVYPSSFIAKQEIK
                     SWPVLGKMGQNAGTVFINRNSRRDIEPINRAVCETLQRGQNVSFFPEARTSSGLGLLP
                     FKAALFQSAIDAGAKVLAVALRYYDETGKRTARPSYADVGLPTCLWRIVSMKKLTIKV
                     DFVCVADAAESEDRYALKDKIEESIRAVVAGDADVAV"
     gene            complement(19186..20013)
                     /gene="mutM"
                     /locus_tag="WNA25_000601"
     CDS             complement(19186..20013)
                     /gene="mutM"
                     /locus_tag="WNA25_000601"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFA9823421.1"
                     /translation="MPELPEVETTLRGIAPHIEGKTVEAVILRQLKLRWQINPDLGEI
                     LSGRQVLSCGRRAKYLIVRFQTGILLIHLGMSGSLRIFTPSDGRIGRPDRHDHVDIVF
                     SDGTVMRYRDPRKFGAILWYEGIEERHPLLEKLGPEPLSEAFCTDYLYAGLKAQKRAV
                     KLALMDNTVVVGVGNIYANESLFRAGISPHRPANRLKKKECAVLVETVKAVLQRAIET
                     GGSTLRDFVDSDGKSGYFQQEYTVYGRHNQPCLRCGGLVVKETLGQRGTFYCTNCQK"
     gene            complement(20066..20731)
                     /locus_tag="WNA25_000602"
     CDS             complement(20066..20731)
                     /locus_tag="WNA25_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFA9823422.1"
                     /translation="MDAWFEDTAMGRYVAKLEQDFFNRYVASYRFSGMCAVQVGGQWL
                     SLSEDIVCVPRDMSMSAENMALADVFADMLLLPHTLECGVPSQILSEAHRILKPSGRL
                     MLTGFNPYSLWGFCRWFDGVRLPEKRFCLPLPELKRQLADAGFDIEFGKFMVYLPPVS
                     SLGQIRFWRFMEKAGDRWWPQCAAVYGLVLVKRAAGVTPLPAWDGYWGGKALAAGAAR
                     VAD"
     gene            20910..22886
                     /locus_tag="WNA25_000603"
     CDS             20910..22886
                     /locus_tag="WNA25_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFA9823423.1"
                     /translation="MSKLKTIALTASGLSVCPGFLYAQNTSSHQVGLAIMRLNSSILD
                     LPPTKQYFQSGSLWDELRQGFRMGEVNPELVRRHESKFIASRSYFDRVVNRSRPYMYH
                     IANEVKKRNMPAEAALLPFIESAFVTKAKSHVGASGLWQFMPATGRHYGLEKTPVYDG
                     RHDVYAATDAALNYLQYLYGLFGDWPLAFAAYNWGEGNVGRAVNRARDQGLEPTYENL
                     RMPNETRNYVPKLLAVRNIIATPQSFGMNISDIDNKPYFQAVEPGRPLDNEAIARLAG
                     ITQSELLALNPAFNVPAFIPKNKRKLLLPVASVQTFQSNYLNAAPDSLFSWEVYTPAA
                     KTSLSDISTATGMSIADIKRLNNLNGNLVNAGRSILVAKNGKTLHTASESVVSIDIDN
                     TPDTYRSNMPAGTVNVSIARIQPAAAQTADITVAPLPQETVRTEPDPLVRIAEPALAT
                     AAAQPQTEKQTAMPSETQTATLAQVVPPNDMQAADELMQLVARNNLRRQAEETISAVI
                     GTPDTVAEHNISSSPQHTVAADGKRRARLETRVAKAADGEAETSPLHASIHRVVEGDT
                     LFNIAKRYNVSVADLIVANNIKGNTIQKGQVLRLAQAAPAQTRIEKVSYTARKGDTFK
                     SIAARFNIHIDDIRRLNPNLNTINPGQRVKLIGS"
     gene            22954..23646
                     /locus_tag="WNA25_000604"
     CDS             22954..23646
                     /locus_tag="WNA25_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFA9823424.1"
                     /translation="MKLIKYLQYQGIGSRKQCQWLIRGGYVSINGTCMDDTDADIDSS
                     YVETLDIDGEAVTVVPEPYFYILLNKPEDYETSHKPKHYRSVFSLFPDNMRNIDMQAV
                     GRLDADTTGVLLITNDGKLNHNLTSPSRKIPKLYEVTLKHPTGETLCETLKNGVPLHD
                     ENETVCAADAVLENPTTLLLTITEGKYHQVKRMVAAAGNRVQHLHRRRFAHLETENLK
                     PGEWKFIECPKF"
     gene            24120..25490
                     /locus_tag="WNA25_000605"
     CDS             24120..25490
                     /locus_tag="WNA25_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HFA9823425.1"
                     /translation="MSSPSNTNRQTWSSRLTYILTVAGATVGFGATWRFPYLVGENGG
                     GAYVFLFCIAMLVIGIPMILVENVIGRRKGVNALDAFGGPMNGKPVAKIWKLVGRMGL
                     LGAFGIMAYYMVLGGWVISYIVNIIGGNLNISSPVDGVVTKGFFTEHIENSPWEIAFY
                     TLLFVAVNQWILVKGVIGGIEKTAKYLMPLLFLFLIAMVVRNVTLPGAMEGVAFYLKP
                     DFSKITAELFVFVLGQVFFALSLGFGVMITLSSYLDKNENLVQTAVITAITNTIIAVL
                     AGFMIFPSLFSFGVAPNSGPTLVFQSLPIVFSHMWAGPVFAVIFFSLLLIAALTTSLT
                     IYEVLITTIQEKTKIRRTAAITIVLSVIFVFGNIPSILSYGPWKDVSVFGKNIFDAFD
                     YISGNILFMLTALGSALFVGFVMKDEAKEELLYKGNHTTVNIWFAYVKYLVPLVILLI
                     FVSNLF"
     gene            25723..26379
                     /locus_tag="WNA25_000606"
     CDS             25723..26379
                     /locus_tag="WNA25_000606"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFA9823426.1"
                     /translation="MNRQKVIAIDGPGASGKGTVAARVAAALGYDYLDTGALYRLTAL
                     YAQKQGVEWHDEENVSALAKKLPAVFSGNRILLDGEDVSDGIRTEAIGMGASAVAQWP
                     KVRAALLQRQRDFLTEKGLVADGRDTGSVVFPQAELKIFLTAESKIRAERRAKQIGIP
                     CEGFTFERILSDIETRDEADRNRKVAPLKQQPDALLLDTSRLTIEETVKKVLDWYRKV
                     "
     gene            26532..28217
                     /gene="rpsA"
                     /locus_tag="WNA25_000607"
     CDS             26532..28217
                     /gene="rpsA"
                     /locus_tag="WNA25_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFA9823427.1"
                     /translation="MSMENFAQLLEESFTLQEMNPGEVITAEVVAIDQNFVTVNAGLK
                     SESLIDVAEFKNAQGEIEVKVGDFVTVTIESVENGFGETKLSREKAKRAADWIALEEA
                     MENGNILSGIINGKVKGGLTVMISSIRAFLPGSLVDVRPVKDTSHFEGKEIEFKVIKL
                     DKKRNNVVVSRRAVLEATLGEERKALLENLQEGSVIKGIVKNITDYGAFVDLGGIDGL
                     LHITDLAWRRVKHPSEVLEVGQEVEAKVLKFDQEKQRVSLGMKQLGEDPWSGLTRRYP
                     QATRLFGKVSNLTDYGAFVEIEQGIEGLVHVSEMDWTNKNVHPSKVVQLGDEVEVMIL
                     EIDEGRRRISLGMKQCQANPWEEFAANHNKGDKISGAVKSITDFGVFVGLPGGIDGLV
                     HLSDLSWTESGEEAVRKYKKGEEVEAVVLAIDVEKERISLGIKQLEGDPFGNFISVND
                     KGSLVKGSVKSVDAKGAVIALSDEVEGYLPASEFAADRVEDLTTKLKEGDEVEAVIVT
                     VDRKNRSIKLSVKAKDAKESREALNSVNAAANANAGTTSLGDLLKAKLSGEQE"
     gene            28228..28524
                     /locus_tag="WNA25_000608"
     CDS             28228..28524
                     /locus_tag="WNA25_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFA9823428.1"
                     /translation="MTKSELMVRLAEVFAAKNGTHLLAKDVEYSVKVLVDTMTRSLAR
                     GQRIEIRGFGSFDLNHRPARIGRNPKTGERVEVPEKHVPHFKPGKELRERVDLA"
     gene            complement(28738..29145)
                     /locus_tag="WNA25_000609"
     CDS             complement(28738..29145)
                     /locus_tag="WNA25_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFA9823429.1"
                     /translation="MTYTTAKAAEKIGISAHTLRFYDKEGLLPNIGRDEYGNRCFTDN
                     DLQWLYLLQCLKNTGMSLKDIKRFAECTVIGDDTIEERLSLFENQIENVKCQIAELKR
                     YLDLLEYKLAFYQKAKALGSVKAVNLPQIPETA"
     gene            29295..30405
                     /locus_tag="WNA25_000610"
                     /pseudo
     CDS             29295..30405
                     /locus_tag="WNA25_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842106.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
     gene            30414..31241
                     /gene="fghA"
                     /locus_tag="WNA25_000611"
     CDS             30414..31241
                     /gene="fghA"
                     /locus_tag="WNA25_000611"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011961786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFA9823430.1"
                     /translation="MKLIEQHQIFGGSQQVWAHHAQTLQCEMKFAVYLPDNRENQPLG
                     VIYWLSGLTCTEQNFITKSSFRRYAAEHQVVVVAPDTGPRGEQVPNGAAYDLGQGAGF
                     YLNATEQPWAANYQMYDYILNELPRLIEEHFPTNGKRSIMGHSMDGHGALVLALRNRE
                     HYQSVSAFSPILSPSLVPWGEKAFTAYLGKDREKWQQYDANSLIQQGYKVQGMRIDQG
                     LEDEFLPTQLRTKDFIETCRAANQPIDVRFHKGYDHSYYFIASFIGEHIAYHAAFLK"
     gene            31771..32922
                     /locus_tag="WNA25_000612"
     CDS             31771..32922
                     /locus_tag="WNA25_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AFG1 family ATPase"
                     /protein_id="HFA9823431.1"
                     /translation="MNNRDQLFKAPPFENHSPLTWYQAASQLPNFIRDDAQAAAIEHL
                     DRLWTELMMFKRKRNRFLGRSLRSPQVPKGLYFYGGVGRGKSFLMDAFFGCLPYRRKR
                     RVHFHAFMAEIHRRLKALKSESNPLKSVAAEITKETRVLCFDEFHVSDIADAMILGRL
                     LENLLNEGVVLVATSNYAPSELYPQGQNRSGFLPTIALIESSLTVLNVDGGEDYRLRT
                     LRPAEIFFTPANEENEAKLAKLFKEMTGITDLNPGISTIHGREIPHKAESGRTIWFDF
                     RALCFSPRSQSDYLYLAEHYEMVFISGLEQLSPQEKAEARRLTWLIDVLYDFRVKLCA
                     TGAVDVNHIYTEGDFAEEFTRTASRMVEMQSEVYLEQPHLTLSPKASGG"
     gene            32981..33406
                     /gene="ndk"
                     /locus_tag="WNA25_000613"
     CDS             32981..33406
                     /gene="ndk"
                     /locus_tag="WNA25_000613"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFA9823432.1"
                     /translation="MAIERTISIIKPDAVGKNVIGKIYSRFEENGLKIVAAKMKQLTL
                     KEAQEFYAVHKDRPFYAGLVEFMTGGPVMIQVLEGENAVLKNRELMGATNPTEAAEGT
                     IRADFATSVSINAVHGSDSVENAALEIAYFFSQTEICPR"
     gene            33550..34644
                     /gene="rlmN"
                     /locus_tag="WNA25_000614"
     CDS             33550..34644
                     /gene="rlmN"
                     /locus_tag="WNA25_000614"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFA9823433.1"
                     /translation="MKTNLLNYDLQGLTRHFADMGEKPFRAKQVMRWMHQSGAQNFDE
                     MTDLAKSLRHKLNEQASIEIPKLMMSQESSDGTRKWLLDVGTGNGVETVFIPESDRGT
                     LCISSQVGCALECTFCSTGRQGFNRNLTAAEIIGQLWWANKAMGVTPKNERVISNVVM
                     MGMGEPMANFDNVVTALSIMLDDHGYGLSRRRVTVSTSGMVPQMDRLRDVMPVALAVS
                     LHASNDEVRNQIVPLNKKYPLKELMAACQRYLVKAPRDFITFEYVMLDGVNDKAQHAY
                     ELIELVKDVPCKFNLIPFNPFPNSGYERSSNENIRIFRDILQQAEFVVTVRKTRGDDI
                     DAACGQLAGQVQDKTRRQQKWQQILIGQQG"
     gene            34647..35408
                     /gene="pilW"
                     /locus_tag="WNA25_000615"
     CDS             34647..35408
                     /gene="pilW"
                     /locus_tag="WNA25_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HFA9823434.1"
                     /translation="MPFKPSKRISLLLVLALGACSTSYRPSRAEKANQVSNIKTQLAM
                     EYMRGQDYRQATASIEDALKSNPKNELAWLVRAEIYQYLKVNDKAQESFRQALSIKPD
                     SAEINNNYGWFLCGRLNRPAESMAYFDKALADPTYPTPYIANLNKGICSAKQGQFGLA
                     EAYLKRSLAAQPQFPPAFKELARTKMLAGQLGDADYYFKKYQSRVEVLQADDLLLGWK
                     IAKALGNVQAAYEYEAQLQANFPYSEELQTVLTGQ"
     gene            35424..36689
                     /gene="ispG"
                     /locus_tag="WNA25_000616"
                     /gene_synonym="gcpE"
     CDS             35424..36689
                     /gene="ispG"
                     /locus_tag="WNA25_000616"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFA9823435.1"
                     /translation="MNTLQRRKTHQVLIDHITVGSEAPVVIQSMTNTDTADAKATALQ
                     IKELSDAGSEMVRITVNSPEAASKVAEIRRRLDDMGYATPLIGDFHFNGERLLAEFPE
                     CGKALSKYRINPGNVGKGVKGDEKFAFMIRTAAENDKAVRIGVNWGSLDQSLAKRMMD
                     ANLVSSAPKPPEEVMKEALIVSALESAEKAVLLGLPEDKIILSCKVSAVHDLIQVYRE
                     LGSRCVYPLHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGSPRTQEV
                     VVGQEILQTMGLRSFTPMVTACPGCGRTTSTVFQELAQDVQNYLRQKMSIWRTLYPGV
                     ESLNVAVMGCVVNGPGESKLADIGISLPGTGETPVAPVYVDGERKVTLKGNNIASEFL
                     AIVEEYVKTNYGKNSSKRNKGKVIPIQSL"
     gene            complement(36743..37357)
                     /gene="clpP"
                     /locus_tag="WNA25_000617"
     CDS             complement(36743..37357)
                     /gene="clpP"
                     /locus_tag="WNA25_000617"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFA9823436.1"
                     /translation="MSFDNHLVPTVIEQSGRGERAFDIYSRLLKERIVFLVGPVTDES
                     ANLVVAQLLFLESENPDKDIFFYINSPGGSVTAGMSIYDTMNFIKPDVSTLCLGQAAS
                     MGAFLLSAGEKGKRFALPNSRIMIHQPLISGGLGGQASDIEIHARELLKIKEKLNRLM
                     AKHCGRDLADLERDTDRDNFMSAEEAKEYGLIDQVLENRASLRL"
     gene            complement(37453..38763)
                     /locus_tag="WNA25_000618"
     CDS             complement(37453..38763)
                     /locus_tag="WNA25_000618"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003742150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFA9823437.1"
                     /translation="MSVTVEILENLERKVVLSLPWSEINAETDKKLKQTQRRAKIDGF
                     RPGKAPLKMIAQMYGASAQNDVINELVQRRFYDVAVAQELKVAGYPRFEGVEEQDDKE
                     SFKVAAIFEVFPEVVIGDLSAQEVEKVTASVGDAEVDQTVEILRKQRTRFNHVDREAR
                     NGDRVIIDFEGKIDGEPFAGGASKNYAFVLGAGQMLPEFEAGVVGMKAGESKDVTVNF
                     PEEYHGKDVAGKTAVFTITLNNVSEPTLPEVDADFAKALGIADGDVAKMREEVKKNVS
                     REVERRVNEQTKESVMNALIKAVELKVPVALVNEEAARLANEMKQNFVNQGMTDAANL
                     DLPLDMFKEQAERRVSLGLILAKLVDENKLEPTEEQIKAVVANFAESYEDPQEVIDWY
                     YADTSRLQAPTSLAVESNVVDFVLGKAKVNKKALSFDEVMGAQA"
BASE COUNT         9852 a        10764 c         9278 g         9061 t
ORIGIN      
        1 gtacagaccc gccgaaattg ttataatcga aaaaattaaa aatcaatacg gattattcta
       61 tcagattgat ttatcgatat ttattatatc attaatatag tttgatttca aaccggcgga
      121 aaatgcgccg cccgccgcag atgcgccctc cccgccatcg gggcgtttaa cgccggattg
      181 ccgatgcggt acaatggcag atatgaagaa aattacccct caaaacctgc gtcccctgct
      241 ttcggaaagc ttgggacata ccgattttgt caacgtcctc aacgcattga ttaaattttt
      301 gcgccgtggc ggcaaaaaat gtgcggggga gcgtttcgac ctgattatcg acacattcaa
      361 acaagacagg gaattactgt cccgcttcag ccggtgtttt tacatctggc tcgcgcaaat
      421 acacatttat ccggcactca tcaaactcgg cattttctcg cgccacagct ttgcccggga
      481 aatgggcata cgcatatacg aacgcttcaa cccgtcatat aaagattttg ccaacttggg
      541 cgaagtcttc ctttatcttt tccattccga aaacgacgac aaatggctgc aaacgctcaa
      601 tatccgccaa tggctggttt tatacgaact catccgtagc cacgccgagc cgtccaaatt
      661 gcagacggcg ggcatccgcc ttgccgatgc gcgtttgcgc gccatcgaaa tgctgtctgt
      721 ctggacggca tccgaagcca tcgaacccga tctcatccgt atcgccccgc gcctgctgga
      781 agccgattct tccttcgtcg ccctccaacg cgaaaccgcc aaactggtcg aacactaccg
      841 caacggcacc gcgccttacg acaccgccca cctcgaagtg atgttcgacc aatgtttcag
      901 ccagattgac tatttgcgtc gcaaagggac gggcgccggc tccggttcgt cggtcaaagt
      961 cgcccacctg ctcgaacgac tccggcagac cgtaggccgt ctgaagctgc tcacagacat
     1021 acaaaccggt gccggcaaca gcaaccgcct gaccatcgcc ctgatgaact ccctcatcta
     1081 cgcggcggtc gaacaataca gcacccgcca cctgcgccgc agcagcatcc gtatgctcgc
     1141 ccgcagcatt accgaaaaca aaagccacca cggcgaacac tacatcaccc gcaaccgcaa
     1201 agaatatttc aaaatgttct actcggcagc aggcggcggc ggcatcatcg ccctgatggc
     1261 gctgctcaaa atccgcatcg gcacactcga cctcagtccc ttcctcgctt ccttgtcggc
     1321 tgggttcaac tacggcatcg gctttatgat catccatatg ctgcactgca ccgtcgccac
     1381 caagcagccc gcgatgactg ccgccagctt tgccgaacag gtcgatctca acgaaggcgg
     1441 caaagcggtg gacaacaaac tctccaagct cctcatcgac gtatgccgct cccaaagcgt
     1501 cgccgtcttc ggcaacgttt ccatcgccat ccttttggcg tgcgccatat cgttcggcta
     1561 tgcccatctg taccggctgc ccatactcga tgcccacacc gccgcctacc agttcaaatc
     1621 catagacatc atcaatcatc ctaccctatg gtatgccgct attgcaggct tgtggctgtt
     1681 ctgctccggc atcatcgcag gttttttcga caaccgcgcc gactacctca acctgcgcca
     1741 acgcctgccc ttcaatccct tgctgcgtaa aatcatgcgc cccgggcccc gccgcgtcct
     1801 cgccgcctac atccacaaac actacggctc gctggtcggc aacttcatct tcggaatgct
     1861 cttgggtatg accggctatt tcggacacct cctcgggctg ccgctggaca tccgccacgt
     1921 cgccttttcc tccgccaacc tcggctatgc cgccgtcagc ggcaacgtcg gctttggtac
     1981 attcgtactc ggcattttca gcgtcctcgc catcggcttg gtcaacctct gcgtcagctt
     2041 cagcctcgcc ctcttcgtcg ccctgcgctc gcgcggcaca aaaatcggca gcatccgcaa
     2101 cctgatcaaa agtttttgga atcagattaa aagcaatcct tgcatacttt tcctcccgcc
     2161 cgccaaagga cagggacatc ctccttcgga caagccttga ccggcaatgc cgtctgaaac
     2221 gggattcgcc ccgaataccg ccctgatgcg ggaaatcccc ataaaaggat gcaaaaatgc
     2281 cgtctgaacc gaaacgtggt tcagacagca ttttaaaaaa cattacaatc ccgggccgcc
     2341 gagcgaattg gcaatgtccc tgaccgccgt tacatacatc cggctgtggt tgtactgcca
     2401 taccgtataa aaattgttca agcccaaata atattcaaac acgccgggtg cggtttccag
     2461 tttgaacaaa accgcctttt catcatctgc gagcgtttcc ccggggatga tgccgtacgc
     2521 cttcaaatcc gccaccgtcc gcgtcagggc ggttttttcg ccaatgattg cctgaacatc
     2581 cgcacccggc gccaacgtcg ccgacaccaa cattttaccg cccgtgcgcc aaccgtgctg
     2641 cttcatataa ttggcaaccg atgccgcgac atcaccgacg ttgccccata tatcccgatg
     2701 tccgtccccg tcataatcca ccgcccattt ccggtagctc gaaggcataa attgcggcat
     2761 acccattgcg cccgcatagc tgcccttaaa ggcgaaaaca tcgccgcctt cttcttttgc
     2821 cagctttaaa agctcgacca attctttttg gaaaaacccg gcgcggcggg gataatcaaa
     2881 gcctaaggtc gccaatgcgt ccgccacacg gaagctgccc gtatttttgc cgtaattcgt
     2941 ttcaatcccg ataatcgcca cgataagctc ggcaggcacg ccgtattttt gcgccacatc
     3001 atcgataacc gcgcggtttt ccgcataaaa cctgcgcgcg ccgtgaaatt tcgccctgcc
     3061 cgaatttccc gtgcggaaca cataccacgg acgcgatgtg gagggtcggt gcataatctt
     3121 gacgatgtcc gccttgtaag ccgctttgtc aaaaaaatcc tgccattccg cctgggaaaa
     3181 atcccctttc ccgacttcat cgtccacaaa acggcggaca tttgcattgg cggcaaaccc
     3241 gctgtcggat accggtacgg ctgccgcgtc aaacgcgggg cggctttctt ttttcatttc
     3301 atccgcgcgg ggggcttggg cttcatttgc ccggggtgtg cgggcctcca tcgccgtaca
     3361 ggcagacaaa gccgccaaac aaattgccag cggcagtatt tttctctttt ccataaacac
     3421 attccgaaca aatagggtaa gtgggaaagc ggcacaaggg tgaggcgcga aatgcggtat
     3481 ccgccgcaat cggcggtttt gcagaatgcc gcgcgttgcc ttttgcgccg cccgaaatct
     3541 gcctgccgcc gtatttgccc gctatccgca atatcggcag tccaatatat ctttgcggat
     3601 gtcgttcagc aggaaggctg tggtgtcttc gtgttcctcc tgtacgaaga caaagagtcg
     3661 ggcgatgatt ttgccgatga agactttttg cagggcgcag gcgtcggtca gggtgtgttc
     3721 ctgcaggaag ctgcggatgt cttcgggcaa agcgtagtcg cgggcaacgg cggcaaagcg
     3781 gacatcggtt tgcaggcggt cgagcagggt ttggtttttg tccaacttaa tgcctgcttc
     3841 tttttcttcg gcggtggcgc ggacgaactg gtcgtaaatt tcggcataaa gcatatcgtt
     3901 cgggccttca aaaatcgtga aggggcggat atcgatggcg atattgccgg ctgggtgtcc
     3961 gcgttcaaaa cccttcgcgc ccaagagttt ttgcaacatt tgcgcggcgg cgtaagtgta
     4021 ttccgtggcg agggttttga cgatgttcgc ctccatcaat tgatgggcga cgggcgcgac
     4081 gggcgaaacg gaatggcaga cgtagcggta aaggatttcg gaaacctgat ggcggcgttg
     4141 gatttcgcgg cgttcgtaat cgacgaatct gatatcgttg cggacatatc ggttcaggtt
     4201 gtcaaggatg tattccataa tgccgtgcgt catgccgatc agttgcaggc ggctgcggat
     4261 aaagatgttt tggaacgcgc gcaaaccggc agcgtcgccc cgggagagtt tcatcacggc
     4321 ggttgcaggc atttcggcat cgatgcggtt gacggcgtaa cggacggcgc gcaggccttc
     4381 ggatgcgagg gtttcgcagc ggatgtatgt tttggggacg agcagcaggt cgatgacttt
     4441 ggcgagtttg ccgtttttgc gctctttggc ggcaacgagg aggaagtcgc tttgcgaatt
     4501 gccctgccag tatttcgcgg cgttgacgta aatggtttgt tcgtcggtat attcgtagca
     4561 ggactgcatt tcgcgtgcaa tcgccgcgcc ggaggtttcg ggttcggtaa cgcccaaacc
     4621 gccgccttcg cctttgaaaa tcatgtccaa accttgtgcg acttgcgctt cgccgccgaa
     4681 ctcttgcaga ggctgcaaca ccagcgcgcc ttcgatgccg gtacgcagcg taacgggcac
     4741 gccgtaatgc cccgcaatcc ttaggacttc ttggatttca aactgactgc ccttgcgccc
     4801 gccgtgtttt ttgtcgagga agggcaacag caaacccgcc tgcttcaagg caagccattt
     4861 gccttcgggc aggtatcgca tcaggtcgat gccgtctgaa aaaatgcggc ggaatgcgga
     4921 ttcgatgtgc tttaaaaaag cagccgtgtc catggttgac ggctgcgcgc tcggttcggt
     4981 gtgtatcatc ggcttcctct gtcggttccc attaatcggc ggccggtcaa accgcctgcc
     5041 acagtttaga gttaattttc taaactttac cacaaagtgc accgggcaac aatccgccgg
     5101 cctttcagac ggcatcgcgc accctcacgt gctaaaatga ccgtttgcat cactgtccgc
     5161 cgattgccgc actatgacct gccccatccc caaaccccgt gaaaaatccc gttggttcaa
     5221 tctttcgcaa ggctcgctgc ccttggcttt ggcgcgttat ttgccgcaca agcggctcaa
     5281 agccgtgctg acccaagatg cggaacaggc gttgcgcctt cagacggcat ggcggttttt
     5341 ccgtccgcac gacacggcgg tgttcctgcc ggactgggaa acgctgcctt acgagcgttt
     5401 ttctccgcat caggatttgg tgtcggagcg gctgtcggcg ttgtggcaga ttaaaagcgg
     5461 cgcggcggac gtgttgttcg taccggttgc cacggcgatg cagaagctgc cgcccgtgcc
     5521 gtttttggcg gggcgcacgt tttggctgaa aacggggcag actttggata taggccgcct
     5581 gaaaaccgat ttggtggatg cgggctacaa tcatgtttcc cacgttgtcg cggcgggcga
     5641 gtttgccgtg cgcggcggca ttgtcgattt gttcccgatg ggcagcgaaa cgccgtaccg
     5701 catcgatttg tttgacgatg aaatcgacag cattaaaacc ttcgataccg acacgcagcg
     5761 caccatttcc cccgtttccg aaatccgcct gctgccggcg cacgagttcc ccaccgacag
     5821 cgaggcgcaa aaaatcttcc gcagccgctt ccgcgaggaa gtcgacggta atccgaacga
     5881 tgccgccgtg tacaaagccg tcagcaacgg ccacttcggc gcgggcgtgg aatattatct
     5941 gccgctgttt tttgaaaacg agctggaaac gctgtttgac tatatcggcg aagatgcgct
     6001 gtttgtctct ttgggcgatg ttcatgccga ggcaaaccgc ttttggaacg acgttaaatc
     6061 acgttacgcc atggcgcagg gcgacgaaac ctatccgcct ttgcttccac agcatttgta
     6121 tctctctgcc gatgtgttcg caggccgtct gaaaaactac ggacaagttc tgcctgatgt
     6181 ttccggcaag gcacactccc tgcccgacct tgccgtcaac cgccaatccg acgagccttt
     6241 gcaggcattg aaggattttc agacggcctt tgacgggcgg attttgctgt gtgccgaaag
     6301 tttgggacgg cgcgaaacca tgctcggttt cttgcagcaa aacggtttga aagccaaacc
     6361 tgtgtccgac tggcagggct tcttgtcggc gcacgagccg ctgatgatta cggtcgcgcc
     6421 gttggcttac ggattcaaac tgggcggact gcaatcttca agccaacagc aaactgttcc
     6481 cgcctccgag ggagaaggca aagcggttac cgaccaaacc gaattttccg catccgcaac
     6541 aaaccctctc cccagccctc tcccgcagga gagggaacaa agtgccgccg ccgtttcaga
     6601 cggcctgaaa gcagccgccg tttcaaccga aagcagcctg tatctcgtcg caagtgatct
     6661 gcacgggcaa acccgacagc aatctgcccc ctcccccgtg ggggagggtt ggggagaggg
     6721 caaagcggtt gccgctcaaa gtgccatcgc cgtcatcacc gaatccgatc tttaccaata
     6781 cgtcgcccgt tcccgcgtcc acaaccgccg taagaaacac gccgctgttt cagacggcct
     6841 gttgcgcgac cttgccgaaa tcaatatcgg cgatcccgtc gtgcacgaag aacacggcat
     6901 cgggcggtat acgggcttgg taacgatgga tttaggcggc gaaaccaacg aaatgatgtt
     6961 gctcgaatac gcgggcgaag cgcagcttta tgtgcctgtt tcgcaactgc atttaatcag
     7021 ccgctactcc ggtcaggcgc atgaaagcgt cgccctgcac aagctcggca gcggcgcgtg
     7081 gaacaaggcg aagcgcaaag ccgccgaaaa agcgcgcgat accgccgccg agttgctcaa
     7141 cctctacgcc caacgcgccg cccaatcggg acacaagttt gaaatcaacg agtcggacta
     7201 tcaggcgttt gccgacggct tcggctacga ggaaaccgaa gaccaggccg ccgccatcgc
     7261 cgccgtgatt aaagatttga cgcaggcgaa gccgatggac cgacttgtgt gcggcgatgt
     7321 cggtttcggc aaaaccgaag tcgccctgcg cgccgcgttt gtggcggtga tgggcggcaa
     7381 acaggtcgcc gtactcgccc cgaccacgct tctggtcgag cagcacgcgc aaaacttcgc
     7441 cgaccgtttc gccgatttcc ccgtaaaagt cgccagcctt tcgcgtttca acaacagcaa
     7501 agccaccaaa gccacgctcg aaggcatggc ggacggtact gtcgatatcg ttatcggtac
     7561 gcacaaattg gtgcaggacg acataaaatt caaaaactta ggtttattga ttatcgatga
     7621 agaacaccgc ttcggcgtgc gacagaaaga gcagctcaaa cgcctgcgcg ccaatgtcga
     7681 catcctaacc atgaccgcca cgccgattcc gcgcaccctc agcatggcgc tcgaaggtct
     7741 gcgcgacttc tcgctgatta ccactgcacc gagccgccgc ctcgccgtga aaacctttgt
     7801 caaaccgttc agcgaaggca gcgtgcgcga agccgtgttg cgcgaactca aacgcggagg
     7861 acaggtattt ttcctgcaca atgaagtaga tacgattgag aatatgcgcg agcggctgga
     7921 aaccctgctg cccgaagccc gtatcggcgt ggcgcacgga caactgcgcg agcgcgagct
     7981 ggaacaagtc atgcgcgact ttttgcagca aagattcaat gtgttgctct gttccaccat
     8041 catcgaaacc ggcatcgaca tccccaacgc caacaccatc atcatcaacc gcgccgacaa
     8101 attcgggctg gcccaactgc accagcttcg cggacgcgtc ggccgcagcc accaccaagc
     8161 ctacgcctac ctgctcacgc ccgaatacat taccaaagac gcagaaaaac gcctcgacgc
     8221 catcgcggcg gcagacgaac tcggcgcagg tttcaccctc gccatgcagg atttggaaat
     8281 ccgaggcgca ggcgaaatcc tcggcgaagg acaatccggc gaaatgatgc aggtcggctt
     8341 cacgctctac accgaaatgc tcaaacaggc cgtgcgcgac ctcaaaaaag gccgccaacc
     8401 cgacctcgac gcaccgttgg gcattaccac cgaaatcaaa ctgcacagcc cggcattact
     8461 gcctgaagac tactgccccg acatccacga gcgcctcgtc ctctacaaac gcctcgccgt
     8521 atgcgaaacc gtgcagcaaa tcaacgccat acacgaagaa ctcgtcgacc gcttcggcct
     8581 gcccgaacaa cccgtcaaaa ccctcattga aagccaccac ctgcggcttg cggcaaaaga
     8641 actgggtatt gatgccatcg atgccacaag tgaagccgta accgttacct tcggcaaaca
     8701 ccactgtatc gacccgacgg ggattatcct gctgattcag acggataaaa aataccggct
     8761 ggcaggggca gacaaattga ggtttgcagc ggaaatggaa aacatcgagg tcaggatcaa
     8821 taccgtcaaa accgtactaa aaaccctgca aggcaaacgc ctgcccaaag gaaattgatg
     8881 taacgatgcc gtctgaagaa caccgttcag acggcataca atcccccaat cctcaccaaa
     8941 aggaacaatc atgtcgattt ttcaccccta tttccaacaa ctgtccaccg aaggttttga
     9001 cggagaagaa gatgtcctct gggaagacga gctgaccgtc aaaggcaatc ttgtcgaggt
     9061 attcctgtag gcagaaaatg aaaacagcct gccaaccgaa acattggatg catttgccgc
     9121 cttcctgtcc gaattggaaa aagccgacga tgctgcacgc gccgcactga cggaatattt
     9181 gaaacgggac agccgctata tcgactttca caccgaagat acccaaacaa gccgtgatgc
     9241 ggcaaaattt gtccgcacta tgcagttgat ctatatcagc ttatgggcaa aaaatccggc
     9301 ttttgccgtt atggattata tgcccgccaa tatggaaagc gacgaaatcc ttgccgtcaa
     9361 actgcatttg gacggcagca ttttctcaat cgattgggag agttgagccg gattgccaat
     9421 cggcaaaaat gaaatgccgt ctgaaaacag cttccctgcc cgaagccttg ccattatgaa
     9481 tttgaaggaa actccactat aatacggcat tcagattttc agacggcatc acgcccgtca
     9541 gaccgcacac aaaccaaaag gaaatacatg ttccgtacta tacttggcgg aaaaatccac
     9601 cgcgccaccg taaccgaagc cgatttaaac tacgtcggca gcattaccgt cgatcaagac
     9661 ctgttagacg cggcaggcat ctgccccaac gaaaaagtcg ccatcgtcaa caacaacaac
     9721 ggcgaacgtt ttgaaaccta taccattgca gggaaacgcg gcagcggcgt gatttgcctg
     9781 aacggtgctg cagccaggct ggtacagaaa ggcgacatcg tcatcattat gtcttatatc
     9841 caactttccg aaccggaaat cgccgcacac gaacccaaag tcgtcttagt ggacggaaac
     9901 aataaaatcc gcgacatcat ctcctacgag ccgccgcaca ccgtactgta atcccgcaaa
     9961 cggacatcga ttatggatat taaaatcaac gacatcaccc tcggcaacaa ttcgcctttc
    10021 gtcctattcg gcggcatcaa cgttttagaa gatttggatt ccaccctcca aacctgtgcg
    10081 cattacgtcg aagttacccg caaactgggc atcccctata tctttaaagc ctctttcgac
    10141 aaggcaaacc gctcgtctat ccattcctat cgcggcgtag gcttggaaga aggcttaaag
    10201 atttttgaaa aagtcaaagc agagttcggc atccccgtca ttaccgacgt acacgaaccc
    10261 catcaatgcc aacccgtcgc cgaagtgtgc gatgtcatcc agcttcccgc ctttcttgcg
    10321 cggcagaccg atttggtggc cgcaatggcg gaaacgggca atgttatcaa catcaaaaaa
    10381 ccccagttcc tcagcccttc gcaaatgaaa aacatcgtgg aaaaattccg cgaagccggc
    10441 aacgggaagc tgattttatg cgaacgcggc agcagcttcg gctacgacaa cctcgttgtc
    10501 gatatgctcg gtttcggcgt gatgaaacaa acctgcggca acctgccggt tattttcgac
    10561 gttacccatt ccctgcaaac ccgcgatgcc ggttctgccg catccggcgg tcgtcgcgca
    10621 caggctttgg atttggcact tgcaggcatg gcaacccgcc ttgccggcct gttcctcgaa
    10681 tcgcaccccg atccgaaact ggcaaaatgc gacggcccca gcgcgctgcc gctacacctt
    10741 ttagaaaatt ttttaatccg catcaaagca ttggacgatt taatcaaatc acaaccgatt
    10801 ttaacaatcg agtaacacgg tttcgcctta tgatgcagac tttccgaaaa atcagcctgt
    10861 atgccgcaac cttgtggctc ggtatgcaga ttatggcagg ttatatcgcc gcaccggtgc
    10921 tgttcaaaat gctgcccaaa atgcaggcgg gcgaaattgc cggcgtattg ttcgacatcc
    10981 tctcttggag cgggcttgcc gtttggggca cggtactggc tgccgccttt gccgccctaa
    11041 cccggcggca aaccgccctg ctgctttttt tattgtccgc ccttgccgcc aaccaatttt
    11101 tggttacacc cgttatcgag gcactgaaat acgggcatga aaattggctg ttgtcggttg
    11161 caggcggatc cttcggaatg tggcacggta tttccagcat gactttcatg gcaaccgccc
    11221 tactttcagc agttttaagt tggcggcttt ccggcaaaga ggccgtctga agccctccca
    11281 ttttttacct cccttcactt cacttggaga acattcatga gcgcaatcgt tgatattttc
    11341 gcccgcgaaa ttttggactc gcgcggcaac cccacagtcg agtgtgatgt attgctcgaa
    11401 tccggcgtaa tgggacgtgc ggccgtaccg agcggcgcat ccaccggtca gaaagaagct
    11461 ttggaacttc gcgacggcga caaatcccgc tattccggca aaggcgtatt gaaggcggtc
    11521 gaacacgtca acaaccaaat cgcccaagcc ctcatcggta tcgatgccaa cgagcaatct
    11581 tatatcgacc aaatcatgat cgaattggac ggtactgaaa acaaaggcaa tttgggtgcg
    11641 aatgcgactt tggcggtctc tatggcggtt gcacgcgccg ctgccgaaga ctcaggcctg
    11701 ccgctttacc gctacttggg cggcgcaggt ccgatgtccc tgcccgtacc gatgatgaac
    11761 gtcatcaacg gcggcgaaca cgccaacaac agcctgaaca tccaagagtt tatgattatg
    11821 cccgtcggcg caaaatcttt ccgcgaagcg ttgcgctgcg gtgcggaaat tttccacgcc
    11881 ttgaaaaaac tgtgcgacag taaaggcttc ccgaccacag tcggcgacga aggcggtttc
    11941 gcccccaacc tgaacagcca caaagaagcc ctgcaactga tggtcgaagc ggccgaagcc
    12001 gccggctaca aggcgggcga agacgtatta ttcgcattgg actgcgcgtc cagcgagttc
    12061 tacaaagacg gcaaatacca cttggaagcc gaaggccgct cctacaccaa cgcggaattt
    12121 gccgaatact tggaaggctt ggttaacgaa ttcccgatta tttccattga agacgggatg
    12181 gacgaaaacg actgggaagg ctggaaactg ctgaccgaaa aattgggcaa aaaagttcaa
    12241 ttggtcggcg acgacttgtt cgtaaccaat ccgaaaattc ttgccgaagg catcgaaaaa
    12301 ggcgtagcaa acgcattgct ggtcaaagtc aaccaaatcg gtactttaag cgaaaccctg
    12361 aaagccgtcg atctggcaaa atgcaaccgc tacgccagcg tgatgagcca ccgctccggc
    12421 gaaaccgaag acagtaccat tgccgacttg gcagtcgcca ccaactgtat gcagattaaa
    12481 accggttctt tgagccgttc cgaccgcatg gcgaaataca accaactgct gcgtatcgag
    12541 gaagaattgg cggaagccgc ctactacccc ggcaaagccg cattctacca actgggcaaa
    12601 taaataaaaa agtggatgta tgtttcaaca cacagggcaa cacatgaagc gtcgccctgt
    12661 gtgttgacct gatttggaag aggttacacc tctcccaaat aaagtctaat tttaccgcca
    12721 cgaaggacgg acatccgagt ggtggggttt caaccagtaa ggaaacgttg atgaagtggg
    12781 taactgtcgt tttatccttc gcacttgtct gttgccaata cagcctgtgg ttcggcaaag
    12841 gcagcatcgg acgcaacagc agtctgagag aacagattgc cgttcaagaa gaaaaaaacc
    12901 agacactcgc cctacgcaat cattcccttg ccgccgaagt ctatgatttg gaaaacggtc
    12961 aagaagccat ttcggaaatc gcccgggtag aactgggtta tatccaagac ggcgaaacct
    13021 tttaccgact catcaggcat aaccggtaat accgtcaaaa agccgtccga accaatgttc
    13081 ggacggcttt tatttcaaca aactgtcaga cagcccctca tcctcccccg ccaaaccgca
    13141 atccagcctg acatccccct cgacgcaaca gcagcacggc agtatctcgt cccgccccaa
    13201 aaaagccaaa ggcggctccc gataagtaac gcttccctcc aaaatcttta cccggcacga
    13261 tccgcaatat ccgcttcggc actgatattc caccatatgc cccgtccgtt ccaagccttc
    13321 caacagggtc tcaccctcca agagctcaaa aaggccctta ttcgtaccaa tgcgcgccat
    13381 ttccgaccaa tcaaaaataa aaggaacaat aaccgatcct acctgctgtt tttcccatca
    13441 tacaacacac aaatgccgtc tgaaacatcc ggcttcagac ggcatttttt caaaaaacat
    13501 ttacaactca aaatcgccca agtcatccgt attcacttca gaatcgattt gaccaatcaa
    13561 ataagaggat atttccactt cctgcggcgc gacctgtacg ttgtcggacg acagccacgc
    13621 attaatccac ggaatcgggt tttgatttgc gccttcaaat ccggccgaca gccccaccgc
    13681 ctgcatacgc agattggtaa tatattcgac gtattgggat aagatttctt tgttcaaacc
    13741 gatcatcgaa ccgtctttaa acaaatatgc cgcccattct ttttcctgtt ccgccgcttt
    13801 tttgaagagt tggaaacatt cgtcctgcaa ctcggcggca atttccgcca tttccggatc
    13861 atcaacaccc gaacgcatca gattaagcat atgctgcgtg ccggtcaggt gcagggcttc
    13921 atcgcgggca atcagtttga tgattttggc gttgccttcc atcaactcac gctcagcaaa
    13981 agcaaacgag caggcaaatg aaacgtagaa acggatggct tccaacacat tgacgcacat
    14041 caggcagaga tagagttttt tcttcaaccc gcgcaaagac acagtaacgg gttttccgcc
    14101 gacattgtgc accccttcgc ccaacaggtt gtaatattgg gtgtattcga ttaaatcatc
    14161 ataatagcag gcaatgtctt cggcgcgggc ggtaatgtat tcgttttcga caatatcatc
    14221 aaacacgacc gacggatcat tcacaatatt gcggatgatg tgggtatagc tgcgcgagtg
    14281 gatggtttcg ctgaagctcc acgtttcaac ccaagtttcc agctcgggaa tcgaaaccaa
    14341 aggcagcaag gcaacattcg gactgcgccc ttggatggaa tcgagcagtg tttgatattt
    14401 cagattgctg atgaaaatat gtttttcgtg ttcgggcagg ttggcgtagt cgatacggtc
    14461 gcgcgacacg tcgatttctt ccggccgcca aaaaaacgac aattgttttt caatcagttt
    14521 ttcaaatacc tcgtatttct gctggtcata acgggcgaca tttaccggct gaccgaaaaa
    14581 catcggctct ttcagcgcgt cgtttttggt tttgggaaag gtgctgtatg acatgactag
    14641 gtgttcgcag gacataacgt ctctcttttt aaaaattagt cgacttttct attaattttt
    14701 aattcaccat ataaaacatg ttgaattcta tcaataaagt gccatcctaa aatagcaaat
    14761 actaaaagaa cactaataaa tatataaaat ttatattcaa ctaagaatga ccgaatgaca
    14821 tccatacttt tccttttctg gaaaataaca acttagcctc atatagtagt aggcactaat
    14881 ttaagaaaat ccttgaagcc tcctactatc tagaagcctt ggatatattt caaaaattaa
    14941 cctgttttaa atcttacaag ccccaccagc gcagccgtca tcttgaatat cggtctgcgt
    15001 gtcgtccgcg ccgtcgcggg tgttgtggta gtacagggtt ttgacgccgt atttgtaggc
    15061 agtcagcagg tctttgagca tttgtttcat agaaactttg ttaccttcga atttgcccgg
    15121 gtcgtaagca gtgttggcgg aaatcgcctg atcgacgaat ttttgcatta cgccgacaag
    15181 tttcaggtag ccttcgttgc ccggaagctg ccacagggtt tcgtaggcat ttttcaaggt
    15241 ttcaaactcc ggcacgactt gtttcaaaat gccgtctttc gatgctttga ccgttaccaa
    15301 tccgcgcggc ggctcgatgc cgttggttgc gttggcgatt tgcgagctgg tttcagacgg
    15361 catgagcgcg gtcagagtag agttgcgcag gccgtatttg acgatgtcgg cacgcaggct
    15421 ttcccagtcg taatgcaaag gctcgccgca gacggcatcc aaatcttttt tgtaggtgtc
    15481 gatgggcagt ttgccttgcg aataaacggt ttggttaaag agcgtgcacg ctccgtattc
    15541 tttggcaagg tttgccgatg ctttgagcag gtaatactgt atggcttcaa aggtacggtg
    15601 ggtcagaccg agcgcggaac cgtcgctgta gcggacaccg tttttcgcca gatagtaggc
    15661 gtagttgatg acaccgatgc cgagcgaacg ccggtccata gtagaggtac gcgcggcttc
    15721 taccggatat ccctgataat ctaaaagtgc atcgagtgcg cgcacggtca aatcggcaag
    15781 cccttccaat tcgtccaagc tgtttaatgc acccaagttg aaggcggaca gtgtacacag
    15841 ggctatttcg ccgtcagggt cgttgatgtt gtccagcggt ttggtcggca gggcgatttc
    15901 catacacaag ttggactgat gaacaggcgc aacgcgcgga tcgaacgggc tgtgcgtatt
    15961 gcagtgatcg acgttttgaa tgtagatgcg cccggtcccg gcacgctcct gcatcagcgt
    16021 ggaaaacagg tcggcagccg gaatgatacg cttgcgtatg ttcgggtctt gctcgtattt
    16081 cgtatagagc cgctcaaatt cgtcttggtc ggcaaaaaac gcttcgtaca accccgaaac
    16141 ctcgttgggc gaaaacagcg taatgttgcc gcccttaatc aggcgggtgt acagcaggcg
    16201 gttgatttgc acgccataat caagctggcg gatacggttg tcttccacgc cccggttgtt
    16261 tttcaacacc agcaggcttt cggcttcaat atgccacaag gggtagaaca aggttgccgc
    16321 gccgccgcgc acgccacctt gcgaacaaga tttgaccgcc gcctgaaaca tcttaaagaa
    16381 gggaatgcag ccggtatgcc gggcttcacc gccccggatt tcgctgtcca gcccgcggat
    16441 acgtccggca ttgatgccga tgcccgcgcg ttgggaaacg tatttcacaa tcgcgctggt
    16501 agtggcattg atggaatcca aactatcgtc gcattcaatc agcacacagc ttgagaactg
    16561 gcgcgtaggc gtacgcacgc cgctcataat cggcgtgggc aacgatactt tgaatgtgga
    16621 aacggcatcg taaaaccgtt tgacgtaacc caagcgcacc tctttcgggt atttgctgaa
    16681 aaggcacatc gccaccaaaa catataaaaa ctgcggcgtt tcgtaaatct ggcgggtaac
    16741 gcggttctgt accagatatt tgccttcgag ctgtttgacg gcggcatagg aaaaggacat
    16801 atcgcgttcg tggtcgatat aggcgttcag ttcgtcaaat tcttcgcggc tgtaatcctc
    16861 aaggatatgc ctgtcgtatt ttccggcatc ggtgagcttt ttaacatggt tgtaaaggtg
    16921 cggcggctcg tattcgccgt aggctatttt acgaagatgg aaaatcgcca aacgtgcggc
    16981 aaggtattgg tagtccgggg tatcttccga aattaaatct gcagcggctt tgatgatggt
    17041 ttcgtggatg tcgtcggtgc ggatgccgtt gtagaactgg atgtgcgatt tcaactcgac
    17101 ctgcgacacg gaaacatttt ccaatccttc cgccgcccaa gtaacgacac ggtgaatctt
    17161 atccaaatca atggcttcca atcttccgtc tcgtttggtt acttttaaat cagtcggtgt
    17221 attcatcgct tcctcttcca ctcttgatat tcaagacacg gtcttttcaa ataaattaag
    17281 gcagacaata tagtggattt ttagcatttt ctccagtctt gacaacgatt gcatttttca
    17341 gtttcaggca atgcgcgata aaacccgcct gtcgtatttt tcctataaca tttgttttat
    17401 ctgataattc tttaccgata aaaaacgggt aaattttttg ccttttgacc ggctccggct
    17461 acaaggcgat gaaataagga ttttccgacg aaaaaggaaa acttccggtt ttccgcccct
    17521 gcgaattgtt aaattttgca aagtatgatt tttgccacgc cgccgccgac aaattccatt
    17581 ttcttaccga ttggaattta ttattgagat taatatgtta tttgaatttg catatcaaac
    17641 ggcaagtttt gtcggctgaa tgagtctgaa actgccgaca gtttgcccgt tccatccttc
    17701 catcctatac tggaaggaat tacaaactga aaggatcgtc atgtccgtca ataaagatgc
    17761 ttgccatact gttgtctgca atactgggac tggtatcaac aactgccgct gccggtacgt
    17821 cagaacccgc ccaccgacat accaaacata tcagcaaggc aaacaagcag atgctgcacc
    17881 ccgaatgcag gaaatatttg gaacgccgtg ccgcgtggta ccgatcgcaa ggcaacgtgc
    17941 aggaattgcg cgaaaacaaa aaggcgcgca aagcattccg caccctgcct tatgcggaac
    18001 agaaaatcca atgccgggcg gcttatgagg ctttcgatga tttcgacggc ggcaggttcc
    18061 gccgttaatc ccatataaaa tatgctgtct gaacacaagt taagatggca ttttccatag
    18121 atatagtgga ttaaatttag accggtacgg cgttgcctcg ccttgccgtg ctatttgtac
    18181 tgtctgcggc ttcgccgcct tgtcctgatt taaatttaat ccactataca acaaactatc
    18241 cttacggcat ttcttttgag gtactcccgt taccgcagcc ttaaggcatc cgcaaaagcg
    18301 aatcataccg ccacattccg acaaccgctt tcagacggca acatccgcat cgcctgcgac
    18361 aacggcacgg atgctttctt cgattttatc ttttaaagca taacggtctt cgctttccgc
    18421 cgcgtccgcc acgcaaacga aatcgacttt gatagtcagt tttttcatag acacgattcg
    18481 ccacaggcaa gtcggcaaac cgacatcggc atacgacgga cgggccgtcc tttttcccgt
    18541 ttcgtcataa taacgcagtg cgaccgccaa aacctttgcc cccgcatcga tggcggattg
    18601 gaacagcgca gccttgaacg gcaggagccc cagtccggag gaagttcgcg cctcagggaa
    18661 aaaactgacg ttttgaccgc gttgcaaggt ttcgcagacg gcgcggttaa tcggttcgat
    18721 gtcgcgccgc gaattgcggt tgatgaacac cgttcccgcg ttctgcccca tcttgcccaa
    18781 taccggccag cttttgattt cctgcttggc gataaagcta ctcggataaa ccgcgctcat
    18841 cgcgaaaata tccagccagg acacgtggtt ggcggcaacc aagacaccgt tcggatgttc
    18901 gggtgcgggt ctgcccacct ccaatccgat atccaaagcc gccaaagcgc ccttgcccaa
    18961 cgctataact gcccgattgc gcgacttggg gcagccgccg tcaataccgc gcaggttttt
    19021 gccggttttg aacagccaga ccgtcaagcg gcacaagcgg cgcagacgtg taaaaaatga
    19081 agctttattt gaagacatgg gcaattcttt cccgataatc ggtgtttgtt gttgaaacgt
    19141 tcggacggca taccgataaa atgccgtcag aaaccgtttt ccgccctatt tctgacagtt
    19201 cgtgcaataa aacgtgccgc gctgccccaa agtttctttc acaaccaaac cgccgcaccg
    19261 gaggcacggc tgattgtgcc gcccgtacac cgtatattcc tgttgaaagt agccgctttt
    19321 gccgtcgctg tccacaaaat ccctcagcgt actgccgccc gtttcaatgg cgcgctgcaa
    19381 caccgctttg acggtttcaa ccaaaaccgc gcactctttc ttttttaggc ggttggcagg
    19441 acggtggggc gaaatgcccg ccctgaacag actctcgttg gcataaatat tgcccacgcc
    19501 gaccacgacg gtattgtcca tcagagcgag tttgaccgcg cgtttctgcg ccttcagccc
    19561 cgcatacaga taatccgtgc aaaatgcctc cgacaaaggc tccggcccca gtttttccaa
    19621 cagcggatga cgttcttcga ttccctcata ccaaagtatc gcgccgaact ttctcggatc
    19681 gcggtaacgc atgaccgtgc cgtctgaaaa cacaatatcg acgtgatcgt gtctgtccgg
    19741 cctgccgata cgtccgtccg acggcgtaaa gatccgcaag ctgcccgaca tccccaagtg
    19801 aatcagcaaa atccccgttt ggaaacggac aatcagatat ttggcacgcc tgccgcagga
    19861 caaaacctgc cggccggaca aaatctcccc caaatcggga ttgatctgcc agcgcagctt
    19921 cagttggcgc aatatcacgg cttccaccgt ttttccttca atatgcggcg cgatgccgcg
    19981 caacgtcgtt tccacttccg gcaattccgg cataacccct cccgacattt cttctgacag
    20041 atgccgtctg aaagacggcc gtcccttaat ccgcaaccct tgccgcaccc gccgcaaggg
    20101 ctttgccgcc ccaatacccg tcccacgcgg gcaggggcgt tacgcccgcc gcccttttga
    20161 ccaaaaccaa accgtacact gcggcacatt gcggccacca acggtcgccc gccttttcca
    20221 taaaccgcca aaagcgtatt tgcccgagcg acgaaacggg aggcagatac accatgaatt
    20281 tcccaaattc aatatcgaaa ccggcatcgg caagctgcct tttcaactcg ggcagcggca
    20341 gacaaaaccg tttttccggc agacggacac cgtcaaacca acggcagaat ccccaaagcg
    20401 aatacggatt gaaacccgtc agcatcaaac gtccggacgg tttcaatatc cggtgcgctt
    20461 ccgacaggat ttgcgaaggt acgccgcatt ccagcgtatg cggcaaaagc agcatatccg
    20521 caaaaacatc cgccaaagcc atattctccg ccgacatcga catatctcgc ggcacacaga
    20581 caatgtcttc agacaggctc agccactgac cgcccacctg aaccgcacac attcccgaaa
    20641 aacggtatga cgccacatac cgattaaaga agtcctgttc caattttgca acataccgcc
    20701 ccatcgccgt atcttcaaac catgcatcca tattgcgtcc gtttcaaaca gattgcctgc
    20761 cgatatttta ttccaaacag gatttctgtc aaaaaacaca tcggccgccc atttccgaat
    20821 ccgcataaag ttcctgtaaa acttgacgct ttttcagtca aacagtacca tcggacgata
    20881 aaatatgttt attccgccga gtataaatca tgtccaaact caaaaccatc gccctgaccg
    20941 catcaggtct gtccgtttgt ccgggtttcc tatatgccca aaacacctca tcacaccaag
    21001 tcggtttagc gattatgcgg ttaaactctt caatactcga cctgccaccg acaaaacaat
    21061 atttccaatc cggcagcctg tgggacgagc tgcgccaagg cttccggatg ggcgaagtca
    21121 atcccgaact ggtacgccgc cacgaaagca aattcatcgc aagccgcagc tatttcgaca
    21181 gggtcgtcaa ccggagccga ccctatatgt accatatcgc caacgaagtc aaaaaacgca
    21241 atatgcccgc cgaagccgcc ctgcttccct tcatcgaaag cgcgttcgtc accaaagcca
    21301 aatcacacgt cggcgcatcg ggcctgtggc agttcatgcc cgctaccggc aggcattacg
    21361 gcttggaaaa aacaccggtt tacgacggca ggcacgacgt ttacgcagcc accgatgccg
    21421 cactcaacta tctgcaatat ctctatggac tgttcggcga ctggccgctc gcctttgccg
    21481 cctacaactg gggtgaaggc aacgtcggac gcgccgtcaa ccgcgcccgc gaccaagggc
    21541 tcgaaccgac ctacgaaaac ctgcgtatgc ccaacgaaac gcgcaactat gtccccaagc
    21601 tgctcgccgt gcgcaacatt attgccaccc cccaatcttt cggcatgaat atcagcgaca
    21661 tagacaacaa accctatttt caggcagtcg aaccgggccg tccgctcgac aacgaagcca
    21721 tcgcccggct tgccggcatc acgcaaagcg agctgctcgc cctgaatcct gcattcaacg
    21781 tccccgcgtt catccccaaa aacaaacgca aactgctgct tcctgtcgcg tccgtccaaa
    21841 ccttccaaag caactacctc aacgccgcac ccgacagcct gttttcatgg gaagtctata
    21901 cgcctgccgc caaaaccagc ctgtccgaca tctcgacggc aaccggcatg agcattgccg
    21961 acatcaaacg cctcaacaac ctgaacggca accttgtcaa cgcaggacgc agcatccttg
    22021 tcgccaagaa cggcaagacc cttcatacgg catcggaatc cgtcgtttcc atcgacatcg
    22081 acaatacgcc cgacacctac cgttccaata tgccggcagg cacggtgaac gtcagcattg
    22141 cccgaatcca acccgccgcc gcacagacag cggacattac cgtcgcacct ttgccgcaag
    22201 aaaccgtccg tacggaaccc gatccccttg tccgcattgc cgaacccgcc cttgcgacag
    22261 ccgcagcgca acctcaaacc gaaaaacaga ctgccatgcc gtctgaaacc caaaccgcaa
    22321 cactcgcgca ggtcgtcccc ccaaacgaca tgcaggcggc agacgaactc atgcagcttg
    22381 ttgcccgaaa caacctgcgc cggcaggctg aagaaaccat ctccgccgtc atcggcacgc
    22441 ctgacacagt tgccgaacac aacatttcct catctccgca acataccgtt gccgccgacg
    22501 gcaaacgccg ggcacgtttg gaaacgcgcg tagccaaagc tgccgacggc gaagccgaaa
    22561 cctccccgct ccatgccagc atccaccgcg ttgtagaagg cgacaccctg ttcaacattg
    22621 ccaaacgcta caacgtcagc gtagccgacc tgattgtcgc caacaacatc aaaggcaaca
    22681 ccatccaaaa aggacaggta ctccgcctgg cgcaggcagc ccctgcccaa acccgtattg
    22741 aaaaagtatc ctacaccgcg cgcaaaggcg acaccttcaa aagtatcgcc gcgcgcttca
    22801 atatccatat cgacgacatc cgccggctca atcccaacct gaacaccatc aatccgggac
    22861 agagggtcaa actgattgga agctgattcg gatacggcac atgacaggac tttctcagtc
    22921 ctgtcttttt catcccacat ttcccatacc atcatgaaac tcatcaaata cctgcaatat
    22981 caaggcatag gaagccgcaa gcagtgccaa tggctgatta ggggcggcta cgtctccatc
    23041 aacggaacct gcatggacga caccgatgca gacatcgatt cctcatacgt cgaaacgttg
    23101 gatattgacg gggaagcagt aaccgtcgtt cccgaaccct atttctacat cctgctcaac
    23161 aagcctgaag attacgaaac ttcgcacaaa ccgaaacact accgcagcgt attcagtctg
    23221 ttccccgaca atatgcggaa catcgatatg caggcggtcg gcaggctgga tgcggatacg
    23281 accggcgtat tgctgattac caacgacggc aaactgaacc acaacctgac ttcgccgagc
    23341 agaaaaattc ccaagctata cgaagtaacg ctcaaacacc ctacaggaga aacgctctgc
    23401 gaaactttga aaaacggcgt gccgctccac gacgaaaacg aaaccgtttg tgccgccgat
    23461 gccgttttgg aaaatccgac caccctgctg ctgaccatta ccgaaggaaa ataccaccaa
    23521 gtcaaacgca tggttgccgc cgccggcaac cgcgtgcaac accttcaccg ccggcgattc
    23581 gcacatttgg aaacagaaaa cctcaaaccc ggggaatgga aatttatcga atgtccgaaa
    23641 ttctgaaaca aaattccata atttccctat attcaaccat atgcttatta aataagcagt
    23701 tttacaaaaa ttaactcaaa aaacagttag tttacaatat aatccgcttg tctaagtaac
    23761 gcctgttcaa gcaggattat gagcaagacg ttttccccgt aatgtgtttt gccatctata
    23821 cttcccccct tgtatagatg gttttctatt tcaaattccc tctctttcat catcattgaa
    23881 atgctatcat taatatgatt aacaaaattt cacttccgta caccgatgta gaaaaataac
    23941 agattaatct gttcataaac tccgattctt cctcatcggt ttatactcat cagaaatatt
    24001 cactgaaata tatgcatttt acatggaaaa tcactaggca gttgaaaaac attcagtcaa
    24061 tttctgttaa gaatcacgtg taaaatttcc gctttatctc aatcggacac ggtttcaata
    24121 tgtcttcccc ttcaaatacc aaccgtcaaa cctggtccag ccgtttgact tatatcctta
    24181 ccgttgccgg cgcgactgtc ggtttcggcg cgacgtggcg tttcccgtat ttggtcggtg
    24241 aaaacggcgg cggcgcgtat gtgtttttat tctgtatcgc gatgctggtt atcggcatcc
    24301 cgatgatttt ggtggaaaac gtcatcggac ggcgcaaagg cgtgaacgcg ctggatgcgt
    24361 tcggcggccc gatgaacggc aaacctgttg ccaaaatttg gaaactggtc ggccggatgg
    24421 ggctgctcgg cgcgttcggc atcatggctt attatatggt actcggtggc tgggtaatca
    24481 gctatatcgt taatattatt ggaggaaatt tgaatatttc cagccccgtc gacggtgtgg
    24541 ttacaaaagg cttctttacc gaacacattg aaaacagccc ttgggaaatt gcgttttata
    24601 cgctgctttt tgtcgccgtg aaccaatgga ttttggtcaa aggcgttatc ggcggcattg
    24661 aaaaaacggc gaaatacctg atgcctctgc tgtttttgtt cctaatcgcg atggtcgtcc
    24721 gcaacgttac ccttccgggc gcaatggaag gggttgcttt ctatctgaaa cctgatttca
    24781 gcaagattac cgccgaactg ttcgtcttcg ttttgggaca ggtatttttt gccctgagcc
    24841 tgggtttcgg cgtgatgatt accttatcca gctatctgga taaaaacgaa aatctggttc
    24901 agacggcagt tatcacggca attaccaata ccatcatcgc tgtacttgcg ggctttatga
    24961 ttttcccgtc gctcttcagc ttcggcgtcg cgcccaattc cggcccgact ttggtgttcc
    25021 aaagtttacc gattgtgttc tcacatatgt gggcaggacc tgtgttcgcc gtcattttct
    25081 tctctctgct cctgattgcc gcgttgacaa cttcgctgac gatttatgaa gtgttgatta
    25141 cgaccattca ggaaaaaacc aaaatccgcc gtaccgccgc aatcacgatt gtattgtccg
    25201 tcatcttcgt tttcggcaac atcccgtcta ttttgagcta tggcccgtgg aaagacgttt
    25261 ccgtgttcgg caaaaatatt ttcgatgcct tcgactacat cagcggcaac atcttgttta
    25321 tgctgaccgc gctcggttcc gcattgtttg tcggttttgt gatgaaggac gaagcgaagg
    25381 aagaattgct ttataaaggc aaccatacga cggtcaatat ttggtttgcc tatgtgaaat
    25441 accttgtgcc gctggtgatt ctgctgattt tcgtcagcaa cctgttctaa tccgcagcaa
    25501 tcaatgccgt ctgaaggtca tacctctttc agacggcatt gcattgatgc ccgccacgcc
    25561 ggaatccgaa ccggcttgcc ggcttctgtc ggacgctttc cgggcaggcg gcgttccgtc
    25621 tgcttagaca atcgtccttt aaaacaggta gaatccgccc caacgggaaa cacacccttc
    25681 agacggcaaa acccataccc caaaccatca ggaatccccc ttatgaacag acaaaaagtc
    25741 atcgccatcg acggtccggg cgcatcgggc aaaggcacgg tcgccgcccg cgttgccgcc
    25801 gcattgggat acgattatct cgataccggc gcgctctacc gcctgaccgc cctatatgca
    25861 caaaaacaag gcgtggaatg gcacgatgaa gaaaacgttt ccgcacttgc caaaaaactg
    25921 cccgccgtat tttcaggcaa ccgcatccta cttgatggcg aagacgtttc agacggcatc
    25981 cggacagaag ccatcggtat gggcgcatcc gcagttgcgc aatggcccaa agtccgcgcc
    26041 gcactgctgc aacgccaacg cgattttctg accgaaaaag gactggttgc cgacggacgg
    26101 gacaccggat cggtcgtctt cccccaagcc gaactcaaaa tttttctgac agccgaatct
    26161 aaaatccgtg ccgaacgccg cgccaaacaa atcggcattc cctgcgaagg tttcacattc
    26221 gagcgcatcc tgtccgacat cgagaccaga gacgaagcag accgaaaccg caaggttgcc
    26281 cccctgaaac aacagcccga tgccctgctt ttggatacga gccgcctaac tatagaagaa
    26341 actgtaaaaa aagtgcttga ttggtatcgt aaagtttaaa ttttcaggta taatcgcccg
    26401 cattgcgctt cagacagcat gaatttccat gtgccgtctg aaaccttatt tacccgcctg
    26461 ccgtgccaag gactgcagac acccaccaac ccaacccgca ccccttggcg gtgtaccgaa
    26521 aagagttata tatgtctatg gaaaattttg ctcagctgtt ggaagaaagc tttaccctgc
    26581 aagaaatgaa cccgggtgag gtgattaccg ctgaagtagt ggcaatcgac caaaacttcg
    26641 ttaccgtaaa cgcaggtctg aaatcagaat ccctgatcga tgtagctgaa ttcaaaaacg
    26701 ctcaaggcga aattgaagtt aaagtcggcg acttcgttac cgttaccatc gaatccgtcg
    26761 aaaacggctt cggcgaaacc aaactgtccc gcgaaaaagc caaacgcgca gccgattgga
    26821 tcgctttgga agaagccatg gaaaacggca acatcctgtc cggcatcatc aacggtaaag
    26881 tcaaaggcgg cctgaccgtt atgatcagca gcatccgcgc attcctgccg ggttctttgg
    26941 tcgacgtacg tcccgttaaa gacacttccc attttgaagg caaagagatc gaattcaaag
    27001 tgatcaaact ggacaaaaaa cgcaacaacg tcgttgtttc ccgccgcgcc gttttggaag
    27061 ccactttggg tgaagaacgc aaagccctgc tggaaaacct gcaagaaggc tccgtcatca
    27121 aaggcatcgt caaaaatatc accgactacg gcgcattcgt tgacctgggc ggcatcgacg
    27181 gcctgctgca catcaccgat ttggcatggc gtcgcgtgaa acacccgagc gaagtcttgg
    27241 aagtcggtca ggaagttgaa gccaaagtat tgaaattcga ccaagaaaaa caacgtgttt
    27301 ccttgggtat gaaacaactg ggcgaagatc cttggagcgg tctgacccgc cgttatccgc
    27361 aagccacccg cctgttcggc aaagtatcca acctgaccga ctacggcgca ttcgtcgaaa
    27421 tcgaacaagg catcgaaggt ttggtacacg tctccgaaat ggactggacc aacaaaaacg
    27481 tacacccgag caaagtcgta caactgggtg acgaagtcga agtcatgatt ttggaaatcg
    27541 acgaaggccg ccgccgtatc tctttgggta tgaaacaatg ccaagccaat ccttgggaag
    27601 aatttgccgc caaccacaac aaaggagaca aaatctccgg tgcggttaaa tccattaccg
    27661 atttcggcgt attcgtcggc ctgcccggcg gcatcgacgg tctggttcac ctgtccgacc
    27721 tgtcttggac cgaatccggc gaagaagccg tacgcaaata caaaaaagga gaagaagtcg
    27781 aagccgtcgt attggcaatc gatgtggaaa aagaacgcat ctccttgggt atcaaacaac
    27841 tggaaggcga tcctttcggc aacttcatca gcgtgaacga caaaggttct ttggttaaag
    27901 gttccgtgaa atctgttgat gccaaaggcg ctgttatcgc cctgtctgac gaagtagaag
    27961 gctacctgcc tgcttccgaa tttgcagccg accgcgttga agacttgacc accaaactga
    28021 aagaaggcga cgaagttgaa gccgtcatcg ttaccgttga ccgcaaaaac cgcagcatca
    28081 aactttccgt taaagccaaa gatgccaaag aaagccgcga agcactgaac tccgtcaatg
    28141 ccgccgccaa tgcgaatgcc ggtaccacca gcttgggcga cctgctgaaa gccaaactct
    28201 ccggcgaaca agaataaggt tgcagacatg acaaagtctg agttaatggt tcgcttggca
    28261 gaagtatttg ccgccaaaaa cggcacgcat cttctggcaa aagacgtaga gtacagcgta
    28321 aaagtcttgg ttgacaccat gacccgatcg cttgcccgag gtcaacgcat cgaaatccgc
    28381 ggtttcggca gcttcgattt gaaccatcgt cctgcccgca tcggtcgcaa tcccaaaacc
    28441 ggcgagcgcg tggaagtacc tgaaaaacat gtaccccact tcaagcctgg taaagaattg
    28501 cgcgagcggg tcgacttggc ttgaaaagaa aatgccaatt aaaccttagc atcaaaacgc
    28561 cgctggtacg cggcgttttt tctttggttt aacttcatcc gctgcttcaa taccttaagc
    28621 caagcaagca acggattaga gcgtggattt tttaccattg cttacaatcc gctttttaaa
    28681 caacaaattg ttgatttcta ttacgaacag gacaaaatcc tgcttattgc actaaaacta
    28741 agccgtttca ggaatttgcg gcaaatttac agcttttacc gagcctaatg ctttcgcttt
    28801 ttggtaaaac gccaatttgt attcaagcaa atctaaataa cgttttaatt cggcaatttg
    28861 acacttcaca ttttctattt gattttcaaa caaggaaagg cgttcttcaa tggtatcgtc
    28921 gccaatgacg gtacattccg caaagcgttt gatgtctttt aagctcattc ccgtattttt
    28981 caagcattgc aataagtaca accattgcaa atcgttatcg gtaaaacaac ggttaccgta
    29041 ttcatcacgt ccgatattag gcaacaagcc ttctttgtcg taaaagcgta aagtgtgggc
    29101 ggagatgcct attttttcgg cagctttggc agtggtataa gtcattttcc ctccctctaa
    29161 acaaaaaacg gtaaaaaaac acttgcctta gagtgaactc taaaatgtaa actgttgcta
    29221 tgttgctcag gcaaggctat tttgttaatg aattaagagg aaagacaatg gaaatgaaac
    29281 aaaccgattc aaccatcaaa tctcgtgcgg cggtggcatt cgcccctaac caacccttac
    29341 aaattgtgga aatcgacgtg gaaatgccgc gtaaagacga ggtgttaatt cgtaacaccc
    29401 acactggcgt gtgccatact gatgcattta ccttatcagg aagcgatcct gaaggcgtat
    29461 tccctgtggt gcttggacac gaaggtgcgg gtgtggtcgt tgctgtgggc gagggtgtgt
    29521 caagcgtaaa accgggtaat cacgtcattc cgctttacac cgcagaatgt ggcaaatgtg
    29581 agttttgcct ctcgggtaaa accaatttat gcgtctcagt gcgtgataca caaggtaaag
    29641 gcttaatgcc agacggcacg acgcgttttt cttatcaagg tcagccgatt tatcactata
    29701 tgggctgttc gactttcagt gaatattctg ttgttgccga agtttcactg gcgaaaatca
    29761 acccgaaagc caaccacgaa caagtatgtt tgctcggctg cggcgttacc actggtattg
    29821 gtgcggtaca taacacggca aaagtacaag aaggcgattc tgttgccgtg tttggcttgg
    29881 gagcgattgg tttggctgtg gtgcaaggtg cgcgtcaagc caaagccggt cgcattatcg
    29941 ccattgatac caatcctgca aaattcgagt tggcaaaaca gtgttggtgc aactgactgt
    30001 ttgagcccga aagattacga taaaccaatc aaagatgtgt tgttagacat caacaaatgg
    30061 ggcattgacc acacctttga atgtatcggc aatgtaaacg taatgcgtca ggcattagaa
    30121 agtgcacacc gaggttgggg acaatccatc atcatcggcg tagcggatgc aggacaagaa
    30181 atttcaacgc gtccgttcca atcggtaaca ggccgtgttt ggaaaggctc ggcatttggt
    30241 ggtgttaaag gccgctccga gcttccgaaa atggtggagg attcaatgaa aggcgatatc
    30301 caattagaac catttgtgac ccatgcaatg acccttgatc aaatcaatga agcctttgag
    30361 ttaatgcacg aaggtaaatc gatccgcgct gttattcatt actaaggtcc acgatgaaac
    30421 tgattgaaca acatcaaatt tttggtggtt cgcaacaagt ttgggcgcat catgcccaaa
    30481 cgcttcaatg cgaaatgaaa tttgccgtct atttgccgga taatcgggaa aatcaaccgc
    30541 ttggtgtgat ttattggctt tccggcttga cgtgtacgga acaaaatttc attaccaaat
    30601 cgagcttccg gcgttatgcg gcagaacatc aagtggttgt ggtcgccccc gataccggcc
    30661 ctcgcggaga gcaagtgccg aacggtgccg cttatgattt aggacagggg gcaggctttt
    30721 atttgaatgc gaccgaacag ccttgggcgg cgaattatca aatgtatgat tacattttga
    30781 acgagttgcc ccgtctgatt gaggagcatt ttcctaccaa cggcaaacgt tccattatgg
    30841 ggcattcgat ggacggacac ggcgcattgg tattggcact acggaatcgg gaacattatc
    30901 aaagtgtttc tgccttttcg cctattttat cgccaagcct cgtaccgtgg ggagaaaaag
    30961 cctttactgc ttatttaggg aaagaccgtg aaaaatggca gcaatatgat gctaactcac
    31021 tcattcaaca aggctataaa gtgcaaggta tgcgtatcga tcagggcttg gaagatgagt
    31081 ttttgccgac acaattgcgt accaaagatt ttatcgaaac ctgtcgtgcg gcaaatcagc
    31141 caatcgatgt gcgtttccat aaaggatacg atcacagcta ttacttcatc gccagtttta
    31201 ttggtgagca tatcgcttat cacgccgcat ttttgaagta agtcaaagag cggtcagttt
    31261 tcaaagcagt ttggaatagc cggcacgagg gcggtaagaa gtgccggcat aaacgtatgc
    31321 cgcctgaacc agaaggagcc ggccctacgg attaccaaac cctgcccttg atttgattag
    31381 tatgattttt aagcttaaac cctgattgat caaacattaa aatcgcccgt atttcctact
    31441 tcactttcgg gcttcgtatt attcgttgcc gaatttgcat aagcaacctg ttcctgttgt
    31501 gttttcgccc cctccaagct gacggtggcg taacagcttc gcgattcacc atctgtttca
    31561 tttgtcctcc ttcaatatta aaacgcagcc tgaaataaaa ggcattttgg ctcatatctg
    31621 caccatatta aaacgccgcc tttgcttata ccccctttgt gcgcgtcatt attcttttcc
    31681 acgggaaacg ccaagtttga aggaaatcat ttataatacc agcggtaagc attttctttc
    31741 ttagccgcaa gaagtataac aaggttaaat atgaataata gagaccaact ttttaaagcc
    31801 ccgccgtttg aaaaccacag cccgctgacc tggtatcagg ctgcctcaca actgcccaac
    31861 ttcatccgcg acgacgcaca ggcggccgcc atcgaacacc ttgaccggct ttggaccgaa
    31921 ttgatgatgt tcaaacgcaa aagaaaccgt tttttaggca ggagtttgcg ttccccgcaa
    31981 gttcccaaag ggctttattt ctatggcgga gtcggacgcg gcaagagctt tctgatggac
    32041 gcttttttcg gctgcctccc ttaccgccgc aaacgccgcg tccactttca tgcctttatg
    32101 gcggaaatcc accggcggct gaaagccctg aaaagcgaaa gcaacccgtt gaaatccgtt
    32161 gccgccgaga ttaccaaaga aacccgagta ttgtgttttg acgaatttca tgtcagcgat
    32221 attgcggatg caatgatttt aggccgtctg ctggaaaacc tgcttaacga gggcgttgtt
    32281 ttggtggcga cttcaaacta cgcgccttcc gaactctacc cgcaaggtca aaaccggagc
    32341 ggttttcttc ccacaatcgc gctcatcgag tccagtctga ccgtcttaaa cgttgacgga
    32401 ggggaagact accggctgcg taccctccgc cccgccgaga ttttctttac gcctgccaat
    32461 gaagaaaatg aggcaaaact ggcaaaactg ttcaaagaaa tgacaggcat taccgatttg
    32521 aaccccggca tcagcaccat ccacggccgg gaaattcccc acaaagccga gtccggtcgt
    32581 accatatggt ttgatttccg cgcactgtgc ttcagtcccc gctcacagtc cgactatctg
    32641 tatttggccg aacattatga aatggttttt atttcaggtt tggaacaact ctcaccgcaa
    32701 gaaaaggcgg aggcgcgacg gctgacttgg ctgattgacg tactctacga tttccgggtc
    32761 aaactgtgtg ccaccggcgc ggtagatgtc aaccatatct acacggaagg cgattttgcc
    32821 gaagaattta cccgcaccgc cagccggatg gtcgaaatgc agtccgaagt ttatttggaa
    32881 cagccgcacc tgaccttatc tcccaaggct tcaggcggat aagttatttt tttggtagaa
    32941 taccgatttg attcttctta agtaaaaata aggatatagc atggcgattg aacgtaccat
    33001 atccatcatc aaacccgatg ccgtcggcaa aaacgttatc ggcaaaatat acagccgctt
    33061 tgaggagaac ggtctgaaaa tcgttgccgc caaaatgaag cagcttaccc tcaaagaagc
    33121 ccaagaattt tatgcggttc ataaagaccg ccccttctat gccggattgg ttgaatttat
    33181 gaccggcggt ccggttatga ttcaagtact ggaaggcgaa aacgccgttc tgaaaaaccg
    33241 cgaactgatg ggggcgacca atcccaccga agccgcagaa ggcacgatac gcgcggactt
    33301 tgccacttcg gtcagcatta acgccgtaca cggttccgac agtgtggaaa atgccgcttt
    33361 ggaaattgcc tactttttca gccaaaccga aatctgcccc cgttgataca atacaccgcc
    33421 caattcctat tcagacggca taaaatatat ccatgccgtc tgaaaactct gttgcaaaag
    33481 acttcaaatc aaacttgcct gccctgcaat tttttatttg aagccttaat ttaagaaaaa
    33541 cacaaataca tgaaaaccaa tctgctcaac tacgaccttc aagggctaac ccgacatttt
    33601 gccgatatgg gtgaaaaacc attccgcgcc aaacaggtta tgcgttggat gcaccaatcc
    33661 ggcgcgcaaa attttgacga aatgaccgat ttggcaaaat cgttgcgcca taaactgaac
    33721 gaacaggcaa gcatcgaaat tcccaagctg atgatgtccc aagaatcttc agacggcact
    33781 cgaaaatggc ttttggatgt cggtacgggg aacggcgtgg aaaccgtctt catccccgaa
    33841 tcggatcgcg gcacgctctg catttcctcg caagtcggct gcgctttgga atgtacattt
    33901 tgttcgaccg gccggcaggg cttcaaccgc aatttaaccg ctgccgaaat catcggacag
    33961 ttgtggtggg caaacaaagc gatgggcgtt acaccgaaaa acgagcgtgt gatttccaac
    34021 gtcgtcatga tgggcatggg cgagccgatg gcgaacttcg acaatgtcgt taccgccttg
    34081 agcatcatgc tggacgacca cggctacggt ttgagccgcc gccgcgtaac cgtttccact
    34141 tcgggtatgg ttccccaaat ggacaggttg cgcgatgtca tgccggtggc cttggcggtt
    34201 tccctccacg cttccaatga cgaagtccgc aaccaaatcg taccgttgaa caaaaaatat
    34261 cccttgaaag aattgatggc cgcatgccaa cgctatctgg tcaaagcgcc cagggatttc
    34321 atcactttcg agtatgtgat gctggacggt gtcaacgata aggcacaaca tgcatacgaa
    34381 cttattgaac tggtcaaaga tgttccctgc aaattcaatt taatcccgtt taatcctttc
    34441 cctaattcgg gatatgaacg ttcaagtaac gaaaacatcc gtattttcag agatatcctg
    34501 caacaagccg aatttgtcgt taccgttcga aaaactcgtg gcgacgatat cgatgccgcc
    34561 tgcggacagt tggcaggtca ggttcaggat aaaacgcgcc gccaacaaaa atggcagcag
    34621 attttaatcg gacaacaggg gtaattatgc cttttaagcc atccaaacga atctctttat
    34681 tacttgttct tgccttgggc gcgtgcagca cttcctaccg cccctcgcgg gcagaaaaag
    34741 ccaatcaggt ttccaatatc aaaacccagt tggcgatgga atatatgcgc ggtcaggact
    34801 accgtcaggc aacggcaagt attgaagatg ccttgaaatc gaaccctaaa aacgagcttg
    34861 cctggctggt ccgtgccgaa atctatcaat acctgaaagt taacgacaag gcgcaggaaa
    34921 gtttccggca agccctctcc atcaaacccg acagtgccga aatcaacaac aactacggct
    34981 ggttcctgtg cggcaggctc aaccgccctg ccgaatctat ggcatatttc gacaaagccc
    35041 tggccgaccc cacctacccg accccttata ttgccaacct gaataaaggt atatgcagcg
    35101 caaaacaggg gcaattcgga ttggcggaag cctatttgaa acgttccctc gccgcccagc
    35161 cgcagttccc acccgcattt aaagaactgg cgcgcaccaa aatgctggcc gggcagttgg
    35221 gcgatgccga ttactacttt aaaaaatacc aaagcagggt agaagtcctt caggccgatg
    35281 atttgctgct aggctggaaa attgccaaag ccctcggcaa cgtgcaggcg gcatacgaat
    35341 atgaagcaca attgcaggca aatttcccct actcggaaga attgcaaacc gtcctcaccg
    35401 gtcaataaac agattcaaac catatgaaca cactccaacg ccgcaagacg catcaagtcc
    35461 tcatcgatca tatcaccgtc ggttcagaag cacccgtcgt tatccaatct atgaccaaca
    35521 ccgacactgc cgatgcaaaa gccaccgcat tgcagattaa ggaattgagc gatgccggct
    35581 cggaaatggt gcggattacc gtcaacagcc ccgaagccgc gtccaaagtt gccgaaatcc
    35641 gccgccgctt ggacgatatg ggctatgcca caccactgat tggcgatttc cattttaacg
    35701 gcgaacgcct gttggcggaa tttcccgaat gcggcaaagc attgtccaaa taccgcatca
    35761 atcccggcaa tgtcggcaaa ggcgtaaaag gcgatgaaaa atttgccttt atgattcgga
    35821 ctgctgctga aaacgataaa gccgtccgta tcggcgtaaa ctggggttct ttggatcaga
    35881 gcctcgccaa acgtatgatg gatgccaacc tcgtttcttc cgcgccgaaa ccgcccgaag
    35941 aagtgatgaa ggaagcactg attgtctccg ctttggaatc tgccgaaaaa gccgttctat
    36001 tgggactgcc cgaagacaaa atcatcctgt cgtgcaaagt cagcgcggtt catgatttga
    36061 ttcaggttta ccgcgaactg ggcagccgtt gcgtctatcc gctgcatttg ggtttgaccg
    36121 aagccggtat gggcagcaaa ggcattgtcg catcaacggc ggcattatct gttttgcttc
    36181 aagaaggaat tggcgacacc atccgcattt cactcactcc ggaaccgggc agcccgcgta
    36241 ctcaggaggt cgtcgtcggg caagagattt tacagactat gggtttgcgc tcgtttacgc
    36301 cgatggttac cgcctgcccg ggatgcgggc gtaccaccag taccgtattt caagagctgg
    36361 cacaagatgt tcaaaattac ctgcgccaaa aaatgtctat atggcgtacc ctttatcccg
    36421 gggttgaatc cctgaacgtt gccgtaatgg gctgcgttgt caatggcccc ggagaaagca
    36481 aattggccga catcggaatc agccttcccg gtacggggga aacacccgtc gcacctgttt
    36541 atgtagatgg ggagcgcaaa gtcacactga aaggtaataa tattgcaagc gaattcctag
    36601 ctattgtaga ggaatatgtt aaaaccaatt atggcaaaaa cagttctaaa cgcaataaag
    36661 gaaaagtcat cccgatacag tctctataaa acaaatgccg tctgaacgtt tcagacggca
    36721 tttgtttctc tcagatttct tattaaagcc gcaaagaagc gcggttttcc aaaacttggt
    36781 cgatcaaacc atattctttt gcttcttcgg cagacatgaa attatcacgg tcggtgtcgc
    36841 gctccaaatc tgccaaatcg cggccgcaat gtttcgccat caggcggttg agtttttctt
    36901 tgattttcaa caactcgcgt gcgtggattt caatgtcgga tgcctgaccg cccaagccgc
    36961 cgctgattaa aggctggtga atcataatcc ggctgttggg cagggcgaaa cgtttgcctt
    37021 tctcgcctgc cgacaataag aacgcgccca tacttgccgc ctgccccaag cacaaagtcg
    37081 atacatcggg cttgatgaaa ttcatcgtgt cgtaaatcga cataccggcc gttaccgagc
    37141 cgccggggga attgatgtag aagaaaatat ccttatccgg attctcactt tccaaaaaca
    37201 acagttgggc gaccaccaga ttagcagact catcggttac cgggccaacc agaaatacga
    37261 tgcgctcttt caaaagccgg gaatagatat cgaatgcacg ctcaccgcga ccgctctgct
    37321 cgataacggt agggacaaga tggttatcaa aagacatttc gtctcctttc atgatggaaa
    37381 agcaccaaag cgggctttaa aaacggcttc ggtgctttca aaaactgcct tcagacagca
    37441 ttttcagaac aatcaggctt gcgcgcccat cacttcgtca aaagacaaag cttttttgtt
    37501 tactttggct ttgcccaaaa cgaaatcaac aacgttgctt tctactgcca aagaagtcgg
    37561 ggcttgcagg cgggaagtat ctgcgtagta ccagtcaatc acttcttgag gatcttcgta
    37621 gctttctgcg aagttggcga caacggcttt gatttgctct tcagtcggtt ccagtttgtt
    37681 ttcgtcaacc agtttggcca aaatcagacc caaagatacg cgacgttcgg cttgttcttt
    37741 gaacatatcc aaaggcaaat ccaagttcgc ggcatcggtc ataccttggt taacgaagtt
    37801 ttgtttcatt tcgtttgcca ggcgggcggc ttcttcattg accaaagcaa ccggaacttt
    37861 caactctacg gctttaatca gcgcgttcat tacggattct ttggtttgtt cgttcacgcg
    37921 gcgttccact tcgcggctta cgtttttctt cacttcttca cgcattttgg caacatcgcc
    37981 atccgcaata cccaaggctt ttgcaaaatc tgcatcgact tcaggcagag tgggctcgga
    38041 aacattgttc agcgtaatgg tgaacacggc agttttaccg gcaacatctt tgccgtggta
    38101 ttcttcaggg aagttgacgg taacgtcttt actttcgccc gctttcatgc cgactacgcc
    38161 ggcttcaaat tcaggcagca tttgacctgc gcccaatacg aaggcgtagt ttttggatgc
    38221 gccgccggca aaaggttcgc cgtcgatttt gccttcaaag tcgatgatga cgcggtcgcc
    38281 gtttcgggct tcgcggtcaa catggttgaa gcgggtacgt tgtttacgca ggatttctac
    38341 agtttggtcg acttcggcat cgccgacgga agcggttact ttttcgacct cttgtgcaga
    38401 caaatcgccg ataacgactt cggggaacac ttcaaaaatg gcggcaactt tgaaagactc
    38461 tttatcgtct tgttcttcaa cgccttcaaa acgtggatag cctgccactt tcaactcttg
    38521 ggcaacggca acatcgtaga agcggcgttg caccagctcg ttgatcacgt cgttttgcgc
    38581 gctcgcaccg tacatttggg caatcatttt taaaggtgct ttacccggac ggaaaccgtc
    38641 gatttttgca cggcgttggg tttgtttcag ttttttatcg gtttctgcgt tgatttcgga
    38701 ccaaggcagg gacaacacta ctttgcgttc cagattttct aaaatttcaa cagttacgct
    38761 catcataagc ccttaaattt gttgtgttga taaaatgata aaccttcttc cctgcatggg
    38821 gaagcaaaca gcgcaacggt acgatatttg aaccgcattg ccgcaaaggg gaaattttag
    38881 ctggcaagta tatcacaatg tttcgcctga aacataatat gccgagacct ttgcaaaatt
    38941 cccctaaaat cccct
//
LOCUS       DAWTQS010000012        38532 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.012, whole genome shotgun sequence.
ACCESSION   DAWTQS010000012 DAWTQS010000000
VERSION     DAWTQS010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 38532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38532
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.012"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            42..2219
                     /locus_tag="WNA25_000619"
     CDS             42..2219
                     /locus_tag="WNA25_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HFA9823438.1"
                     /translation="MTRFKYSLLFAALLPVYAQADVSVSDDPKPQESTELPTITVTAD
                     RTASSNDGYTVSGTHTPFGLPMTLREIPQSVSVITSQQMRDQNIKTLDRALLQATGTS
                     RQIYGSDRAGYNYLFARGSRIANYQINGIPVADALADTGNANTAAYERVEVVRGVAGL
                     PDGTGEPSATVNLVRKHPTRKPLFEVRAEAGNRKHFGLGADVSGSLNAEGTLRGRLVS
                     TFGRGDSWRQLERSRDAELYGILEYDIAPQTRVHAGMDYQQAKETADAPLSYAVYDSQ
                     GYATAFGPKDNPATNWSNSRNRALNLFAGIEHRFNQDWKLKAEYDYTRSRFRQPYGVA
                     GVLSIDHSTAATDLIPGYWHADPRTHSASMSLTGKYRLFGREHDLIAGINGYKYASNK
                     YGERSIIPNAIPNAYEFSRTGAYPQPSSFAQTIPQYDTRRQIGGYLATRFRAADNLSL
                     ILGGRYSRYRAGSYNSRTQGMTYVSANRFTPYTGIVFDLTGNLSLYGSYSSLFVPQLQ
                     KDEHGSYLKPVTGNNLEADIKGEWLEGRLNASAAVYRARKNNLATAAGRDQSGNTYYR
                     AANQAKTHGWEIEVGGRITPEWQIQAGYSQSKPRDQDGSRPNPDSVPERSFKLFTAYH
                     LAPEAPSGRTIGAGVRRQGETHTDPAALRIPNPAAKARAVANSRQKAYAVADIMARYR
                     FNPRTELSLNVDNLFNKHYRTQPDRHSHGALRTVNAAFTYRFK"
     gene            complement(2482..2805)
                     /locus_tag="WNA25_000620"
     CDS             complement(2482..2805)
                     /locus_tag="WNA25_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HFA9823439.1"
                     /translation="MDIVQLPSAALKAWMDEGRMFCLLDVRTDEEAAVCSLPNALHIP
                     MNLIPLRQNELPDDVPLVVYCHHGIRSLHTAMYLAEAGFENLYNLQGGIDAWAVEVDA
                     EMARY"
     gene            complement(2886..3542)
                     /locus_tag="WNA25_000621"
     CDS             complement(2886..3542)
                     /locus_tag="WNA25_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFA9823440.1"
                     /translation="MDFEKARFNMVEQQIRPWDVLDFDVLDALAEIPRELFVDEDLQG
                     LAYADMALPLANGHKMLEPKVVARLAQGLKLTKNDTVLEIGTGSGYATALLAKLAGRV
                     VSDDIDAERQKRAKAVLDGLSLENIDYVQNNGLTELSAGAPFDAVYVGGAVTLVPEVL
                     KEQLKDGGRMAVIVGRRPVQRALLITRRGDVFEEKVLFDTLVAHLDDKDAHPFDSFNF
                     "
     gene            complement(3644..4147)
                     /locus_tag="WNA25_000622"
     CDS             complement(3644..4147)
                     /locus_tag="WNA25_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFA9823441.1"
                     /translation="MHALHFSASDKAALYREVLPQIESVVADETDWVANLANTAAVLK
                     EAFGWFWVGFYLVDTRSDELVLAPFQGPLACTRIPFGRGVCGQAWAKGGTVVVGDVDA
                     HPDHIACSSLSRSEIVVPLFSDGRCIGVLDADSEHLAQFDETDALYLGELAKILEKRF
                     EASRQAA"
     gene            4331..5104
                     /locus_tag="WNA25_000623"
     CDS             4331..5104
                     /locus_tag="WNA25_000623"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFA9823442.1"
                     /translation="MYHQIGMWDQKWVIGNWKMNGRLQNNNSLMHRFRILPTAERVLI
                     GLAAPTVYLLQLHNAMQIVLNNRILTCAQDVSRFPDNGAYTGEVSAEMLADTGTDIVL
                     IGHSERSLYFGEKNEIQRRKMENVLNVGLIPLLCVGESLEEREAGKEHEVIAHQLSIL
                     QGLDTKNIAVAYEPVWAIGTGKVATVEQIADMHAFIYKEILSLCGSDVKIRVLYGGSV
                     KADNAADIFAVPYVDGALVGGASLSYDSFTAIISAAQNA"
     gene            5111..5461
                     /gene="secG"
                     /locus_tag="WNA25_000624"
     CDS             5111..5461
                     /gene="secG"
                     /locus_tag="WNA25_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFA9823443.1"
                     /translation="MEAFKTLIWIVNIISALAVIVLVLLQHGKGADAGATFGSGSGSA
                     QGVFGSAGNANFLSRSTAVAATFFFATCMAMVYIHTHTTKHGLDFSNVQQTQQAPKPV
                     SNTEPSAPVPQQQK"
     gene            5475..5560
                     /locus_tag="WNA25_000625"
     tRNA            5475..5560
                     /locus_tag="WNA25_000625"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5509..5511,aa:Leu,seq:gag)
     gene            6362..6730
                     /locus_tag="WNA25_000626"
     CDS             6362..6730
                     /locus_tag="WNA25_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFA9823444.1"
                     /translation="MRIFKNQWIVKFAKKHKINDSELLEAVERADNGLIDADLGGGVI
                     KQRIARQGQGRSGGYRSLILFKQADRAFFVYAFAKNDRENISDKELDVYRKAAAYYLK
                     YTRAELAALKEDGIITEIEL"
     gene            6727..7038
                     /locus_tag="WNA25_000627"
     CDS             6727..7038
                     /locus_tag="WNA25_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFA9823445.1"
                     /translation="MKYKSEALAAIHEMMEGAYNIGAIDKKTMRGFDKSCLTEIKPLS
                     GGDIKAIREKEALSQAAFAIYLNVGKNHVSAWERGVKKPSGAALKLLTIVKNKGIEAI
                     A"
     gene            complement(7007..7210)
                     /locus_tag="WNA25_000628"
     CDS             complement(7007..7210)
                     /locus_tag="WNA25_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823446.1"
                     /translation="MLSGYAVCTAAVSAWFDAVGHLCGYALIFGSAVLPVLAGIFSSA
                     SIVNLLILPFAKSATQWLRYPCF"
     gene            complement(7808..8363)
                     /locus_tag="WNA25_000629"
                     /pseudo
     CDS             complement(7808..8363)
                     /locus_tag="WNA25_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235198.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8360..9061)
                     /locus_tag="WNA25_000630"
                     /pseudo
     CDS             complement(8360..9061)
                     /locus_tag="WNA25_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950970.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            complement(9298..10089)
                     /locus_tag="WNA25_000631"
     CDS             complement(9298..10089)
                     /locus_tag="WNA25_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFA9823447.1"
                     /translation="MNLFFDTQLGKQQNKATHKIRVMSEAWLEKNGYCPCCGSKPMQR
                     FANNKPVADLFCPNCHEQYELKSKNQKTIGNSVPDGAYRTMLERIRSDTNPNFFFLAY
                     KKADYSIRQLVLVPKHFITPDMIIPRNKGIKNRPHHIMCSINLAPLPESGKIFLIDDS
                     RIIEPETVLKKWQSNLFLRNQNAERKDWLLAVMKCIDQLTEEFTLSQMYEFENKLSIQ
                     FPQNNHIKDKIRQQLQILRDQNMIEFIGRGLYKKIDKLHPTPKAF"
     gene            complement(10318..12948)
                     /locus_tag="WNA25_000632"
     CDS             complement(10318..12948)
                     /locus_tag="WNA25_000632"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFA9823448.1"
                     /translation="MQEHYQPAAIEPAAQKKWDDARISNVSEDASKPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLSRFKLLNGFNVMQPMGWDAFGMPAENAAMKNNVAPAAWTY
                     DNIEYMKTQLKSLGFAVDWEREVATCKPEYYRWEQWLFTKLFEKGIVYRKNGTVNWDP
                     VDQTVLANEQVIDGRGWRSGALIEKREIPMYYFKITDYAEELLNDLDKLEHWPEQVKT
                     MQRNWIGKSRGMTVRFAVSDDSKQGLEGDYAKFLQVYTTRPDTLMGATYVAVAAEHPL
                     ATAAAADKPELQAFIAECKAGSVAEADMATMEKKGVPTGRYVVNPLNGDKLEVWIANY
                     VLWGYGDGAVMAVPAHDERDFEFAAKYNLPKKQVIAVGDNAFDANRWQEWYGDKENGV
                     LVNSGDLDGLDFQTAFDAVAAKLQSQGAGEPKTQYRLRDWGISRQRYWGCPIPIVHCE
                     KCGDVPVPADQLPVVLPENVVPDGMGSPLAKMPEFYETSCPCCGGAAKRETDTMDTFM
                     ESSWYFFRYMSPKFSDGMVSAESAKYWGAVDQYIGGIEHAILHLLYARFFTKLMRDEG
                     LVNVDEPFERLLTQGMVVCETYYRENDKGGKDWINPADVELTFDDKGRPVSAVLKADG
                     LPVVISGTEKMSKSKNNGVDPQELINAYGADTARLFMMFAAPPEQSLEWSDSGVEGAH
                     RFLRRLWRTVYEYLKQGGAVKAFAGNQDGLSKELKDLRHKLHSTTAKVSDDYGRRQQF
                     NTAIAAVMELLNQYDKTDTGSEQGRAVAQEVLEAAVRLLWPIVPHICETLWSELNGAK
                     LWEAGWPTVDEAALVKSEIEVMVQVNGKLRGKITVAADASKADLEAAALANEGAVKFM
                     EGKPAKKIIVVPGRLVNIVV"
     gene            13215..13731
                     /locus_tag="WNA25_000633"
                     /pseudo
     CDS             13215..13731
                     /locus_tag="WNA25_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243014.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
     gene            13738..14181
                     /locus_tag="WNA25_000634"
     CDS             13738..14181
                     /locus_tag="WNA25_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HFA9823449.1"
                     /translation="MPPRLLSGILCCLLTTAPVFAQGQPDAVSAYIQKKKVIVDTSKA
                     ELCFADDRQCHPVLIGTATPKGTFGLTLNSTDKPGYGGEVIGFKQEGDFLFALHRVWN
                     QIPSERRNERIASPSVSDRIMTNGCINVSDAVYEKLRHYFVLEVI"
     gene            14350..16407
                     /gene="ppk1"
                     /locus_tag="WNA25_000635"
     CDS             14350..16407
                     /gene="ppk1"
                     /locus_tag="WNA25_000635"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFA9823450.1"
                     /translation="MTEQNRILCRELSLLAFNRRVLAQAEDKNVPLLERLRFLCIVSS
                     NLDEFFEVRMAWLKRENKLHPRRRPDNGKTPSETIADVTEAARSLIRHQYDLFNNVLQ
                     PELARESIHFYRRRNWTGTQKKWIEDYFDRELLPILTPIGLDPSHPFPRPLNKSLNFA
                     VELDGTDAFGRPSGMAIVQAPRILPRVVPLPSELCGGGHGFVFLSSILHAHVGKLFPG
                     MNVKGCHQFRLTRDSDLTVDEEDVQNLRAAIQNELHDREYGDGVRLEVADTCPAYIRD
                     FLLAQFRLTDAELYQVKGPVNLVRLNAVPDLVNRPDLKFPPHTPGRLKALGKNSPIFD
                     LVRQSPILLHHPYQSFDPVVDMIREAAADPAVLAVKMTIYRTGTRSELVPALMKAALA
                     GKQVTVVVELMARFDEANNVNWAKQLEEAGAHVVYGVFGYKVHAKMALVIRREDGVLK
                     RYAHLGTGNYHQGTSRIYTDFGLITADEQITADVNTLFMEITGLGKPGRLNKLYQSPF
                     TLHKMVIGRIARETEHAKAGKPARITAKMNSLIEPTVIEALYRASAAGVQIDLIVRGM
                     CTLRPGVKGLSENIRVRSIVGRQLEHARVYCFHNNGADDTFISSADWMGRNFFRRIET
                     ATPITAPELKKRVIREGLEMALADNTHAWLMQPDGGYIRAAPAEGESEADLQNDLWDL
                     LRG"
     gene            complement(16556..17659)
                     /locus_tag="WNA25_000636"
     CDS             complement(16556..17659)
                     /locus_tag="WNA25_000636"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFA9823451.1"
                     /translation="MLILQAERDSLLKPLQAVTGIVERRHTLPILSNVLIEGRGGQTK
                     LLATDLEIQIDTAGPEGGAGDFRITTNAKKFQDILRALPAGALVSLDWDDNRLTLKAG
                     KSRFALQTLPAADFPMMNVGEDISATFSLGQERFKTMLSQVQYSMAVQDIRYYLNGLL
                     MQVEGSQLRLVATDGHRLAYAACAIDADLPRAEVILPRKTVLELFKLLNNPDDPIQIE
                     LLDKQVRFQCNGTTIVSKVIDGKFPDFNRVIPLDNDKIFVLSRAELLGALERVSILAN
                     EKFRGARLFLQPGLLSVVCSNNEQEEAREEIEIAYQGGELEVGFNIGYLMDVLRNIHS
                     DDMQLAFGDANRSTLFTVPNNPNFKYIVMPMRI"
     gene            complement(17739..19295)
                     /gene="dnaA"
                     /locus_tag="WNA25_000637"
     CDS             complement(17739..19295)
                     /gene="dnaA"
                     /locus_tag="WNA25_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFA9823452.1"
                     /translation="MTLAEFWPLCLRRLHDMLPHGQFAQWIAPLTVGEEGGVWVVYGK
                     NQFACNMLKSQFAGKIEAVREELAAGRPAFVFKPGEGVRYEMAAVEGAVEPAEPSLHA
                     GSEEMPVQEVLLDELPSEKPVKPAASKTAADILAERMKNLPHEPRQAAGPASRPESAA
                     VAKARTDAQRDAEEARYEQTNLSPDYTFDTLVEGKGNRLAAAAAQAIAENPGQSYNPF
                     FLYGSTGLGKTHLVQAVGNELLKNRPDAKVRYMHSDDYIRSFMKAVRNNTYDVFKQQY
                     KQYDLLIIDDIQFIKGKDRTMEEFFYLYNHFHNEKKQLILTCDVLPAKIEGMDDRLKS
                     RFSWGLTLELEPPELEMRIAILQKKAEAAGISIEDEAALFIANLIRSNVRELEGAFNR
                     VGASSRFMNRPVIDIDLARTALQDIIAEKHKVITADIIIDAVAKYYRIKISDVLGKKR
                     TRNIARPRQVAMSLTKELTTLSLPSIGDSFGGRDHTTVMHGIRAVAKLREEDPELAQD
                     YEKLLILIQN"
     gene            19587..19721
                     /gene="rpmH"
                     /locus_tag="WNA25_000638"
     CDS             19587..19721
                     /gene="rpmH"
                     /locus_tag="WNA25_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFA9823453.1"
                     /translation="MKRTYQPSVTKRKRTHGFLVRSKTRGGRAVLAARRAKGRKRLAV
                     "
     gene            19724..20089
                     /locus_tag="WNA25_000639"
     CDS             19724..20089
                     /locus_tag="WNA25_000639"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFA9823454.1"
                     /translation="MDYRFGRQYRLLKTDDFSSVFAFRNRRSRDLLQVSRSNGNGLDH
                     PRIGLVVGKKTAKRANERNYMKRVIRDWFRLNKNRLPPQDFVVRVRRKFDRATAKQAR
                     AELAQLMFGNPATGCGKQV"
     gene            20154..20375
                     /gene="yidD"
                     /locus_tag="WNA25_000640"
     CDS             20154..20375
                     /gene="yidD"
                     /locus_tag="WNA25_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFA9823455.1"
                     /translation="MNFLLSKLLLGLIRFYQYCISPLIPPRCRYTPTCSQYAVEAVKK
                     YGAFKGLRLAIKRIARCHPFGGHGHDPVP"
     gene            20548..22185
                     /gene="yidC"
                     /locus_tag="WNA25_000641"
     CDS             20548..22185
                     /gene="yidC"
                     /locus_tag="WNA25_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFA9823456.1"
                     /translation="MDFKRLTAFFAIALVIMIGWEKMFPTPKPVPAPQQAAQKQAATA
                     SAEAALAPATPITVTTDTVQAVIDEKSGDLRRLTLLKYKATGDENKPFVLFGDGKEYT
                     YVAQSELLDAQGNNILKGIGFSAPKKQYTLNGDTVEVRLSAPETNGLKIDKVYTFTKD
                     SYLVNVRFDIANGSGQTANLSADYRIVRDHSEPEGQGYFTHSYVGPVVYTPEGNFQKV
                     SFSDLDDDAKSGKSEAEYIRKTPTGWLGMIEHHFMSTWILQPKGGQSVCAAGDCHIDI
                     KRRSDKLYSASVSVPLAAIQAGAKAETAVNLYAGPQTTSVIANIADNLQLAKDYGKVH
                     WFASPLFWLLNQLHNIIGNWGWAIVVLTIIVKAVLYPLTNASYRSMAKMRAAAPKLQT
                     IKEKYGDDRMAQQQAMMQLYKDEKINPLGGCLPMLLQIPVFIGLYWALFASVELRQAP
                     WLGWITDLSRADPYYILPIIMAATMFAQTYLNPPPTDPMQAKMMKIMPLVFSVMFFFF
                     PAGLVLYWVVNNLLTIAQQWHINRSIEKQRAQGEVVS"
     gene            complement(22266..22976)
                     /locus_tag="WNA25_000642"
     CDS             complement(22266..22976)
                     /locus_tag="WNA25_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HFA9823457.1"
                     /translation="MSPVLYLIPTPLGTPDTPCLLQHEQRAVVGLTDFVVEAEKTARA
                     HLKHLGITTPIRELNLQTLNEHTDLKTLPELLKPLQEGRSMGIVSEAGCPAVADPGAN
                     LVALAHKHGFEVRPLVGPSSLLLALMASGANGQNFAFNGYLPSEKNERIQSLNALEQR
                     SRQCGETQIFIETPYRNDALLADAVENLHPETRLCTATDLTLPTQLVVSKTVADWRRM
                     KEMPNLKKRPTIFVMYAG"
     gene            complement(23038..23628)
                     /gene="maf"
                     /locus_tag="WNA25_000643"
     CDS             complement(23038..23628)
                     /gene="maf"
                     /locus_tag="WNA25_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation protein Maf"
                     /protein_id="HFA9823458.1"
                     /translation="MGLELPLVLGTSSVFRREQMERLGIAFQAASPDFDETPMLGESA
                     PQTALRLAEGKARSLTGRFPGALIVGADQVAWCDGRQWGKPMNLANAQKMLMHLSGRE
                     IEFYSAVVLLNTVTGRMHRHIDKTVVVMRQLDELHILRYLEREPDAVYCSCAAKSEGL
                     GALLIERIESTDPNALIGLPVFRLVDFLKNEGVDVL"
     gene            23776..24276
                     /locus_tag="WNA25_000644"
     CDS             23776..24276
                     /locus_tag="WNA25_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFA9823459.1"
                     /translation="MSDPNLIDPEIFAAERQNLQGSFLLEELDERVSLHDYPADRRTK
                     ISFTLTGGRDRLQRLFLDLNVKADMPLICQRCIKPMPFMLDESSRIVLFSDEESLDES
                     MLADEELEGILIEKELDVRALVEDQILMSLPFSPRHGHCGNTLPESANQDKPNPFAVL
                     AGLKSS"
     gene            24310..24489
                     /gene="rpmF"
                     /locus_tag="WNA25_000645"
     CDS             24310..24489
                     /gene="rpmF"
                     /locus_tag="WNA25_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFA9823460.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDALTAPALFVDSTTGEVHRPHHISP
                     NGMYRGRKVVKAKGE"
     gene            24664..25191
                     /locus_tag="WNA25_000646"
     CDS             24664..25191
                     /locus_tag="WNA25_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HFA9823461.1"
                     /translation="MKQKIWYTYDDIHRVIKALAEKIRNAGVKYDAMIAIGGGGFIPA
                     RMLRCFLEIPIYAVTTAYYDSDNEGQVTEEVKKVQWLDPVPEVLRGKNVLVVDEVDDS
                     RVTMEFCLKELLKEDFDTVGVAVLHEKIKAKAGKIPEGIPYFSGITVEDWWINYPWDA
                     LDIDEHNRLAEAGRG"
     gene            25281..26327
                     /gene="plsX"
                     /locus_tag="WNA25_000647"
     CDS             25281..26327
                     /gene="plsX"
                     /locus_tag="WNA25_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFA9823462.1"
                     /translation="MITLAVDAMGGDQGLAVTVPGATAFLQAHPDVRLIMTGDETQLR
                     QALNAAGAPMERIDICHTTQVVGMDESPQSALKNKKYSSMRVAINQVKEGKAQAAVSA
                     GNTGALMATARFVLKTIPGIERPAIAKFLPSDTDHVTLALDLGANVDCTPEQLAQFAV
                     IGSELVHALHPQKGQPRVGLVNVGTEDIKGTDTVKQTYKLLQNSKLNFIGNIESNGIL
                     YGEADVVVADGFVGNVMLKTIEGAVKFMSGAIRREFQSNLFNKLAAVAALPALKGLKN
                     KLDPRKFNGAILLGLRGIVIKSHGGTDKTGFRYALEEAYHEAKSAGLSKIEQGVAEQL
                     AALEAAQNETAASL"
     gene            26465..26716
                     /locus_tag="WNA25_000648"
     CDS             26465..26716
                     /locus_tag="WNA25_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HFA9823463.1"
                     /translation="MKTLEKRMKALDKRMMKFGKSLEGRLDARLIESALDYIHYSERF
                     LAFDILCTYIEDFDIRLTEQESREISFINKEFEIESTSD"
     gene            26743..26961
                     /locus_tag="WNA25_000649"
                     /pseudo
     CDS             26743..26961
                     /locus_tag="WNA25_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218360.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            27115..27381
                     /locus_tag="WNA25_000650"
     CDS             27115..27381
                     /locus_tag="WNA25_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823464.1"
                     /translation="MPIIQTILEGGDIKVKNLKTENYSGKAREILGDSYPIEHISKNI
                     STIQNIISEFIRQKQTPAPNNLMETQQSRCRLKQLGLSDGISFT"
     gene            27333..27536
                     /locus_tag="WNA25_000651"
     CDS             27333..27536
                     /locus_tag="WNA25_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823465.1"
                     /translation="MPSEAAWAFRRHFLHLKQRIGNPAPPVHGNPHLKTICMPNHTPI
                     PVSVICTLPVLGNHRFFAYNSRI"
     gene            27563..28525
                     /locus_tag="WNA25_000652"
     CDS             27563..28525
                     /locus_tag="WNA25_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HFA9823466.1"
                     /translation="MQYAKISGTGSYLPANRVSNDDLAQKVDTSDEWITARTGIKFRH
                     IAAENEKTSDLAAEAARRALADAKLNINDIDLIIVATATPDMQFPSTATIVQQKLGIT
                     NGCPAFDVQAVCAGFMYALTTANAYIKSGMAKNALVIGAETFSRIVDWNDRTTCVLFG
                     DGAGAVVLSASDKPGIIHSKLKADGNYLKLLNVPGQIACGKVSGSPYISMDGPGVFKF
                     AVKMLSKIADDVIEEAGYTAEQIDWIVPHQANRRIIESTAKHLGLSMDKVVLTVQDHG
                     NTSAASIPLALDAGIRSGQIKRGQNLLLEGIGGGFAWGAVLLQY"
     gene            28572..28943
                     /locus_tag="WNA25_000653"
     CDS             28572..28943
                     /locus_tag="WNA25_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFA9823467.1"
                     /translation="MKRQAFFKPMACAAFLSAVSLRLPVLGACYAILSLYAFALYGID
                     KRRAVRGKRRIPEHRLLLPALFGGWTGAYLGSRMFRHKTAKKRFVVLFRLTVSGNVLA
                     TCILIDYFVPPELFVKLGQHL"
     gene            28969..29895
                     /gene="fabD"
                     /locus_tag="WNA25_000654"
     CDS             28969..29895
                     /gene="fabD"
                     /locus_tag="WNA25_000654"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFA9823468.1"
                     /translation="MSFAFFFPGQGSQSLGMMNGFAEHAIVKNTFDEASAILGQDLWA
                     MINGSDAEIIGQTVNTQPIMLAAGVAVYRAYLEVGGKTPAAVAGHSLGEYTALVAAEA
                     LDFADAVKLVRLRAELMQSAVPQGVGAMAAILGLEDEQVRQICAESAQGEVVEAVNFN
                     SPGQVVIAGNAAAVGRTMAAAKEAGAKRALPLPVSVPSHCSLMKPAADKLAEALKTVE
                     IKQPQIRVIHNADVAAYDDAGKIKDALVRQLYSPVRWTETVNALVSDGIAESAECGPG
                     KVLAGLAKRINKAAACSALTDAGQITAFIEAH"
     gene            30034..31899
                     /gene="msbA"
                     /locus_tag="WNA25_000655"
     CDS             30034..31899
                     /gene="msbA"
                     /locus_tag="WNA25_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HFA9823469.1"
                     /translation="MIEKLTFGLFKKEDARSFMRLMAYVRPYKIRIVAALIAIFGVAA
                     TESYLAAFIAPLINHGFSAPAAPPELSAAGIISTLQNWREQFTYMVWGTENKIWTVPL
                     FLIILVVIRGICRFTSTYLMTWVSVMTISKIRKDMFAKMLTLSSRYHQETPSGTVLMN
                     MLNLTEQSVSNASDIFTVLTRDTMIVTGLTIVLLYLNWQLSLIVVLMFPLLSLLSRYY
                     RDRLKHVISDSQKSIGTMNNVIAETHQGHRVVKLFNGQAQAANRFDAVNRTIVRLSKK
                     ITQATAAHSPFSELIASIALAVVIFIALWQSQNGYTTIGEFMAFIVAMLQMYAPIKSL
                     ANISIPMQTMFLAADGVCAFLDTPPEQDKGTLAPQRVEGRISFRNVDVEYRSDGIKAL
                     DNFNLDIRQGERVALVGRSGSGKSTVVNLLPRFVEPSAGNICIDGIDIADIKLDCLRA
                     QFALVSQDVFLFDDTLFENVRYSRPDAGESEVLSALQAANLQSLIDASPLGLHQPIGS
                     NGSNLSGGQRQRVAIARAILKDAPILLLDEATSALDNESERLVQQALERLMENRTGII
                     VAHRLTTVESADRIIVMDGGKIIEQGTHDQLMFQNGYYTMLRNISGKDTAAVQTA"
     gene            31999..33564
                     /gene="guaA"
                     /locus_tag="WNA25_000656"
     CDS             31999..33564
                     /gene="guaA"
                     /locus_tag="WNA25_000656"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFA9823470.1"
                     /translation="MTQDKILILDFGSQVTRLIARRVREAHVYCELHSFDMPLDEIKA
                     FNPKGIILSGGPNSVYESDYQADTGIFDLGIPVLGICYGMQFMAHHLGGEVQPGNQRE
                     FGYAQVKTIDSGLTRGIQDDAPNTLDVWMSHGDKVSKLPDGFAVIGDTPSCPIAMMEN
                     TEKQFYGIQFHPEVTHTKQGRALLNRFVLDICGAQPGWTMPNYIEEAVAKIREQVGSD
                     EVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLNEGKMVMDMFARNLGVKVI
                     HVDAEGQFMAKLAGVTDPEKKRKIIGAEFIEVFDAEEKKLTNAKWLAQGTIYPDVIES
                     AGAKTKKAHAIKSHHNVGGLPENMKLKLLEPLRDLFKDEVRELGVALGLPREMVYRHP
                     FPGPGLGVRILGEVKKEYADLLRQADDIFIQELRNTTDENGTSWYDLTSQAFAVFLPV
                     KSVGVMGDGRTYYYVVALRAVITSDFMTAHWAELPYSLLGRVSNRIINEVKGINRVVY
                     DVSGKPPATIEWE"
     gene            33626..34372
                     /gene="fabG"
                     /locus_tag="WNA25_000657"
     CDS             33626..34372
                     /gene="fabG"
                     /locus_tag="WNA25_000657"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFA9823471.1"
                     /translation="MSTQDLSGKIALVTGASRGIGAAIADTLAAAGAKIIGTATGESG
                     AAAISKRLAQWGGEGRVLNSAEPETVENLIADIEKTFGKLDILVNNAGITRDNLLMRM
                     KEEEWDDIMQVNLKSVFRASKAVLRGMMKQRAGRIINITSVVGVMGNAGQTNYAAAKA
                     GLIGFAKSMAREVGSRGITVNCVAPGFIDTDMTRALPEETRQTFTAQTALGRFGDAQD
                     IADAVLFLASDQAKYITGQTLHVNGGMLMP"
     gene            34437..34922
                     /locus_tag="WNA25_000658"
     CDS             34437..34922
                     /locus_tag="WNA25_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFA9823472.1"
                     /translation="MNTHHHLPCPCGSGTSYAGCCRPLHLRQILPPTAEALMRSRYGA
                     YVLHLIDYIIATTVPAQQTFLDAAELMQWSREAEWLGLNVIAHRNLGKQHAQVEFEAY
                     FQDGGNRTVHHELSAFVKIAEQWYFIDPAVPLPTMKQPCICGSGKKFKACCGKYLQPV
                     A"
     gene            complement(35140..35934)
                     /locus_tag="WNA25_000659"
     CDS             complement(35140..35934)
                     /locus_tag="WNA25_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFA9823473.1"
                     /translation="MLHRLQKVVRHIAQTEIMPRFLNTPSRRKEDGSMLSEADIAAQT
                     AFAAALPLLIDCPMLGEEMSRQEQSALWEQYSGEKGLWIVDPIDGTNNFVNGLPHFAV
                     SVAFVRNGRAELGVIYNPVSGECFYAERGQGAFLNGTRLPLRLVDKKLNEAIAGVEIK
                     YLRSGKLSSRMSTLAPFGTIRSMGSSTLDWCYLACGRYDVYVHGGQKLWDYAAGALIF
                     EEAGGRLTTLEGDGFWSGEHVFKRSVVAALEPKLFERWVGWIRENQ"
     gene            36038..36763
                     /locus_tag="WNA25_000660"
     CDS             36038..36763
                     /locus_tag="WNA25_000660"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFA9823474.1"
                     /translation="MPRFYLPENLSVGQTVDLPDNIVRHLNVLRVRPNENITLFDGKG
                     KAHTARLTVLEKHRAEAEILHEDTTDNESPLNITLIQSISSGDRMDFTLQKSVELGVT
                     AIQPVISERCIVRLDGERAAKRLARWQEIVISACEQSGRNTVPPVLPIIGYREALDKM
                     PSESTRLIMSINRACKLGDIRHPSGAIVFMVGPEGGWTEQEEQQAFEAGFQAVTLGKR
                     ILRTETAPLAAIAAMQTLWGDFT"
     gene            complement(37031..37873)
                     /locus_tag="WNA25_000661"
     CDS             complement(37031..37873)
                     /locus_tag="WNA25_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HFA9823475.1"
                     /translation="MQNHVISLASAAERRAHIADTFGSRGIPFQFFDALMPSERLERA
                     MAELVPGLSAHPYLSGVEKACFMSHAVLWKQALDEGVPYVAVFEDDVLFGKDAEKFLA
                     EDTWLEERFDKDSAFVVRLETMFAKVIVRPDKVLNYENRSFPLLESEHCGTAGYIISR
                     EAMRFFLDRFAVLPAERIKAVDLMMFTYFFDKEGMPVYQVSPALCTQELHYAKFLSQN
                     SMLGSDLEKDREQGRRHRRSLKAMFDLKRALGKFGREKKKRMESQRQAELEKVYGRRV
                     ILFK"
     gene            complement(37892..>38532)
                     /locus_tag="WNA25_000662"
     CDS             complement(37892..>38532)
                     /locus_tag="WNA25_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFA9823476.1"
                     /translation="EVLSEENNKSVLAAIARNGAIWDKPTRHEDIVAVFPFGNPIHNN
                     TMIMRRSVIDGGLRFDPAYIHAEDYKFWYEAGKLGRLAYYPEALVKYRFHQDQTSSKY
                     NLQQRRTAWKIKEEIRAGYWKAAGIAVGADCLNYGLLKSTAYALYEKALSGQDIGCLR
                     LFLYEYFLSLEKYSLTDLLDFLTDRVMRKLFAAPQYRKILKKMLRPWKYRSY"
BASE COUNT         9579 a        11529 c         9240 g         8184 t
ORIGIN      
        1 ctatacaacc ccaccgcaca ccaacacaca aaggaaaccc catgacacgc ttcaaatact
       61 ccctgctttt tgccgccctg ctacccgtgt acgcgcaggc cgatgtttct gtttcagacg
      121 accccaaacc gcaggaaagc accgaattgc cgaccatcac cgttaccgcc gaccgcaccg
      181 cgagttccaa cgacggctac accgtttccg gcacgcacac cccgttcggg ctgcccatga
      241 ccctgcgcga aatcccgcag agcgtcagcg tcatcacatc gcaacaaatg cgcgaccaaa
      301 acatcaaaac gctcgaccgc gccctgttgc aggcgaccgg caccagccgc cagatttacg
      361 gctccgaccg cgcgggctac aactacctgt tcgcgcgcgg cagccgcatc gccaactacc
      421 aaatcaacgg catccccgtt gccgacgcgc tggccgatac gggcaatgcc aacaccgccg
      481 cctatgagcg cgtggaagtc gtgcgcggcg tggcggggct gccggacggc acgggcgagc
      541 cttctgccac cgtcaatctg gtacgcaaac acccgacccg caagccattg tttgaagtcc
      601 gcgccgaagc cggcaaccgc aaacatttcg ggctgggcgc ggacgtatcg ggcagcctga
      661 acgccgaagg cacgctgcgc ggccgcctgg tttccacctt cggacgcggc gactcgtggc
      721 ggcagctcga acgcagccgc gatgccgaac tctacggcat tttggaatac gacatcgcac
      781 cgcaaacccg cgtccacgca ggcatggact accagcaggc gaaagaaacc gcagacgcgc
      841 cgctcagcta cgccgtgtac gacagccaag gttatgccac cgccttcggc ccaaaagaca
      901 accccgccac aaattggtcg aacagccgca accgtgcgct caacctgttc gccggcatag
      961 aacaccgctt caaccaagac tggaaactca aagccgaata cgactacacc cgtagccgct
     1021 tccgccagcc ctacggtgtg gcaggcgtac tttccatcga ccacagcact gccgccaccg
     1081 acctgattcc cggttattgg cacgccgatc cgcgcaccca cagcgccagc atgtcattga
     1141 ccggcaaata ccgcctgttc ggccgcgagc acgatttaat cgcgggtatc aacggctaca
     1201 aatacgccag caacaaatac ggcgaacgca gcatcattcc caacgccatt cccaacgcct
     1261 acgaattttc ccgcacgggc gcctatccgc agccatcatc gtttgcccaa accatcccgc
     1321 aatacgacac caggcggcaa atcggcggct atctcgccac ccgtttccgc gccgccgaca
     1381 acctttcgct gatactcggc ggcagataca gccgctaccg cgcaggcagc tacaacagcc
     1441 gcacacaagg catgacctat gtgtccgcca accgtttcac cccctacaca ggcatcgtgt
     1501 tcgatctgac cggcaacctg tcgctttacg gctcgtacag cagcctgttc gtcccgcaat
     1561 tgcaaaaaga cgaacacggc agctacctga aacccgtaac cggcaacaat ctggaagccg
     1621 acatcaaagg cgaatggctt gaagggcgtc tgaacgcatc cgccgccgtg taccgcgccc
     1681 gtaaaaacaa cctcgccacc gcagcaggac gcgaccagag cggcaacacc tactaccgcg
     1741 ccgccaacca agccaaaacc cacggctggg aaatcgaagt cggcggccgc atcacgcccg
     1801 aatggcagat acaggcaggc tacagccaaa gcaaaccccg cgaccaagac ggcagccgcc
     1861 cgaaccccga cagcgtaccc gaacgcagct tcaaactctt caccgcctac cacttagccc
     1921 ccgaagcccc cagcggccgg accatcggtg cgggtgtgcg ccggcagggc gaaacccaca
     1981 ccgacccagc cgcgctccgc atccccaatc ccgccgccaa agcccgcgcc gtcgccaaca
     2041 gccgccagaa agcctacgcc gtcgccgaca tcatggcgcg ttaccgcttc aatccgcgca
     2101 ccgaactgtc gctgaacgtg gacaacctgt tcaacaaaca ctaccgcacc cagcccgacc
     2161 gccacagcca cggcgcactg cggacagtga acgcggcgtt tacctatcgg tttaaataag
     2221 gtcgtctgaa aacggagttt ctgcgaagct aaaagcagcc tgcacattga aaatgccgcc
     2281 caagcaaacc tgcagtttgc cgccctctcc ctagccttct cccacggggc gagggaattg
     2341 gggtgcaggc tgcctttaag gttcaggcaa attttaactt cgttgatact gcgctttagc
     2401 ttcgcagaag ctgcactttc agacgacctt tatggaacac cagaggtaca cgcatttaag
     2461 gaatgccgtc tgaagccgcc ttcaataccg cgccatttcc gcgtcaacct caaccgccca
     2521 cgcgtcgatg ccgccctgaa ggttgtacag gttttcaaaa cccgcctccg ccaaatacat
     2581 cgccgtatgc aggctgcgga taccgtgatg gcaatacacc acaagcggca catcgtccgg
     2641 caactcgttt tgccgcagcg gaatcagatt catcgggata tgcagcgcat ttggcagcga
     2701 acaaaccgcc gcttcttcat ccgtacgcac gtccaacaaa caaaacatcc gcccttcgtc
     2761 catccacgcc ttcaatgccg ccgacggaag ttgcacaata tccatcacaa accccccgaa
     2821 aaaaaccaag caaaatgccg tctgaagccc caaacccgct ttcagacggc atcacaatcc
     2881 gaacatcaaa aattaaaact gtcgaaagga tgggcatcct tgtcgtccaa atgcgccacc
     2941 aaagtatcga acagcacctt ctcttcaaac acatcgcccc tgcgcgtaat caaaagcgcg
     3001 cgctgcaccg gcctgcgtcc cacaataacc gccatacgcc cgccgtcctt caactgttcc
     3061 tttaacactt caggcacaag ggttaccgcg ccgccgacgt aaaccgcatc aaacggcgca
     3121 cctgcggaaa gttcggtcaa cccgttattt tgcacataat cgatgttttc caaagacaag
     3181 ccgtccaaaa ccgctttggc gcgcttttgc cgttcggcat cgatgtcgtc cgaaaccaca
     3241 cgaccagcca attttgccaa aagcgcggtc gcatagcccg aacccgtgcc gatttccaaa
     3301 accgtatcgt ttttcgtcag cttcaagccc tgcgccagcc gcgccacgac tttcggctcc
     3361 agcatcttat gaccgttggc aagcggcagc gccatatccg catacgccaa accctgcaaa
     3421 tcctcatcga caaaaagctc gcgcggaatc tccgccaaag cgtccaaaac gtcaaaatcc
     3481 aatacatccc acggacggat ttgctgttcg accatattga accgcgcttt ttcaaaatcc
     3541 atttagtgct ccgtcaaata attgtttcaa caatggcagg cattataaaa ccacactccc
     3601 gccgcgccaa cttcggcacg cgccgcccgc ccctttccat cactcaagcc gcctggcgcg
     3661 aagcctcaaa ccgcttctcc aaaatcttcg ccagttcgcc caaatacaaa gcatctgtct
     3721 catcaaactg cgccaaatgt tcgctgtccg cgtccaacac gccgatacag cggccgtctg
     3781 aaaacagcgg cacgacaatc tccgaacgcg acaaagacga acaggcaata tggtcgggat
     3841 gcgcgtccac atccccgacg accaccgtcc cgcccttcgc ccaagcctga ccgcacaccc
     3901 cgcgaccgaa cggaatccgc gtacacgcca aaggcccctg aaacggtgcc aaaaccaatt
     3961 cgtccgaacg cgtatcgacc aaataaaaac ccacccaaaa ccaaccgaac gcctccttca
     4021 aaaccgccgc cgtgttcgcc aaattcgcca cccaatccgt ctcgtccgcc accacagact
     4081 caatctgcgg caacacctcc cgataaagcg cggccttgtc cgaagccgaa aaatgaagcg
     4141 cgtgcatcac atctcctata gttgcataca tatcaggcgg ccattataaa acaacctgcc
     4201 cgaaacaaca ttccaaaccg cccccgcagc cgcccgcccc aagttgcgaa cccgccgcat
     4261 atccactaaa cttcacattg caacgcgcca cacgcggcag acagaaaaaa cacgacacgg
     4321 agcaaaaaag atgtatcacc aaatcggaat gtgggatcaa aaatgggtca tcggcaactg
     4381 gaaaatgaac ggccggctcc agaacaacaa ttcgctcatg caccgcttcc gcatcctccc
     4441 caccgccgaa cgcgtcctca tcggactcgc cgccccgacc gtttacctgc tgcaactgca
     4501 caacgccatg caaatcgtct taaacaaccg catcctcacc tgcgcccaag acgtgagccg
     4561 cttccccgac aacggcgcgt acaccggcga agtgtccgcc gaaatgctcg ccgacaccgg
     4621 cacagacatc gtcctcatcg gacactccga acgcagcctt tatttcggcg aaaaaaacga
     4681 aatccaacgc cgcaaaatgg aaaacgtcct caacgtcgga ctcatcccgt tattgtgcgt
     4741 cggcgaaagc ctcgaagagc gcgaagccgg caaagaacac gaagtcatcg cccatcagct
     4801 ttccatcctg caagggctgg ataccaaaaa catcgccgtc gcctacgaac ccgtctgggc
     4861 gatcggcacc ggcaaagtcg ccaccgtcga acagattgcc gatatgcacg cattcatcta
     4921 caaagaaatc ttgtctttgt gcggaagcga tgttaaaatc cgcgtccttt acggcggaag
     4981 tgtgaaagcg gacaacgcgg ccgacatctt cgcagtacct tatgtggacg gcgcactcgt
     5041 cggcggcgcg tcattgtcgt acgactcctt taccgccatc atcagtgccg cacaaaatgc
     5101 gtagaaaaat atggaagcct tcaaaaccct aatttggatt gttaatataa tttccgcttt
     5161 ggccgtcatc gtgttagtat tgctccaaca cggcaaaggc gcggatgccg gcgcgacttt
     5221 cggatcggga agcggcagcg cgcaaggcgt attcggctct gccggcaacg ctaacttcct
     5281 cagccgctcg accgccgttg cagcaacatt tttctttgca acctgcatgg ctatggtgta
     5341 tattcacacc cacacgacaa aacacggttt ggacttcagc aacgtacaac aaactcagca
     5401 agcacccaaa cccgtaagca ataccgaacc ttctgcccct gttcctcagc agcagaaata
     5461 acagtttttc aaatgccgac atggtgaaat tggtagacac gctatcttga gggggtagtg
     5521 gccgtaggct gtgcgagttc aaatctcgct gtcggcacca acacacaaaa cgcttagaaa
     5581 ttctaagcgt tttttttcgt ttaatcctgt ctaatgccgt caaccaatcc caatcaatac
     5641 aaggatttca ggctgattgt tatcctgccg tcccccttcc tgacagtgca atcccgtcca
     5701 atccgcccta attgaactaa cctgattttt tacagcatcc gaaaataccg cgagaaaata
     5761 ccgtaaaaat aaagctgaac gaccaccaaa tcaggaatgc caagcgggaa atagcttgcg
     5821 gggaatactg acgcaccgta gggaatgagg gggaaaccgt ccaagatgca gagcggtttt
     5881 ttttgggttt ttggaaaaaa cctatactat agtggattaa atttaaaccg gtacggcgtt
     5941 gcctcgcctt gccgtaccgg tttaaattta attcactata aaataagaaa aagataaaaa
     6001 attggtaaca aatgcggtaa caaatggtaa cgaatcggta acaacttttg gggttttccg
     6061 gtttttcacc gtcttggcag tgggagcgta gcggaatgaa aagccaaaac gcacggaacc
     6121 gcgcctattt tgagcaggaa tggcggttaa accgcttggt tatatacggg gcataggaag
     6181 acagcgaaac gcgctaaaaa cgcaatttga gccattaaaa gcgattgatt aaaaaataat
     6241 caggttagcc gccggtgttt caggcggcat aaacggagaa attgcggggc ataaaaaagg
     6301 cagcttgccg tgttgtctgt ctctggtata attccaagta tcactaatca acggctacac
     6361 aatgcggata ttcaaaaacc aatggatagt gaaatttgcc aagaagcaca aaatcaacga
     6421 ttccgagctg ctggaagcgg tagagcgggc ggataacggg ctgatagacg cagatttagg
     6481 cggcggtgtg attaagcagc gcatagcaag gcaaggacaa ggcagaagcg gcggctatcg
     6541 cagcctgata ttgttcaaac aggcagatag ggcatttttc gtttacgcct ttgccaagaa
     6601 cgacagggaa aacatttcgg ataaagaact ggacgtttac cgaaaagccg ccgcatatta
     6661 tctgaaatac acgcgggcag agctggcggc tttgaaagaa gacggcatta tcacggagat
     6721 agaattatga aatacaaaag cgaggcatta gccgccattc atgaaatgat ggaaggggct
     6781 tacaacatcg gcgcaatcga caaaaagact atgcgcggct ttgacaagtc ctgcctgacc
     6841 gaaatcaaac cgttgagcgg cggggacatc aaggcaatca gggagaagga ggcactatcg
     6901 caagccgctt tcgccatcta tctcaacgtg ggaaaaaatc acgtttcggc ttgggagcgg
     6961 ggcgttaaaa agccgagcgg cgcggcgttg aagctgctga ccatcgtcaa aaacaagggt
     7021 atcgaagcca ttgcgtagcc gacttggcaa acggcaaaat cagcaagttc acaatagacg
     7081 cgctgctgaa tatgcctgcc aagacaggca agaccgccga accgaatatc agggcatagc
     7141 cgcacaaatg cccgaccgca tcaaaccaag ccgaaacggc ggcggtgcag acggcatagc
     7201 ccgacagcaa ggcacggcgc agacgggcgc gaaacccgaa acatccccga ccgcgaggca
     7261 cggggatttt ttgcgcccgt tgcaggggga ttagatttaa gcggcgcggg cttgaaggca
     7321 aaacgggcgg ggcgcagaac tgtttaaatg ccgtctgaat ctcaaacgat ttcagacggc
     7381 attttgaaac aacggctcaa attctcgatc cccttccctt aacgccgacg ttttttatta
     7441 acgcgcccct tatttctgac actttgctca taaaccggca taacggtcgg caacaaccgt
     7501 tttagatttt ctatacaggc attgtttaca gcatcagcat aagacaccgt actctggaat
     7561 accgcctaaa ccctgcggat agttttggat gtggtatcga cattgtcggc agacttgttt
     7621 aactcgaccg ttttcaaata atctttgaca gatgctgcat tcagtgtcgc ggaatccgcg
     7681 tattttgaac aaaaggtcgg gctactttat cacaatagca gaaagatgga aaacggcggg
     7741 gattggtgaa aggtcgtctg aaatgatatt cagacgacct tgaacggttg tgttttaaat
     7801 atggtattta ttcatttgtt tcaccatcag gcagtccaaa ccaccttaag ggaatcgtgt
     7861 taaattggta atggcagtta acactgataa tataatctaa aacatcttta atattccggt
     7921 tttgtaaacc aatcattcaa aacatagaca tatccaaccc gccaacctaa agagtgaact
     7981 aactttgtat attgttttct cttaaaatcg caagtttgta atttctcatc tactgaaccg
     8041 ggagtttgtt ggaatttgat ctcaataata aataaggtat cacggatgat gataaataag
     8101 ccattatcgg gcagtaatct tttagacaga tggtctttcc aattaatatt gtattccagt
     8161 ctttccaaaa atcggtaaaa ctcatatttt ttgaaacaat aagccttcaa ttctccatta
     8221 aaccaaactt cataacccgc attatcatct gtttttctta agtcgtaccc gggaatttct
     8281 tcaaacaact ttcgtaagtc tgtacgttct tcgaatctga gtcctgtttg tgtccttgca
     8341 ccgccgattc cgccaataat catttttcta accttttctt tgctaattca aaaaaatctt
     8401 cttccagttc gcaaccgcaa aatctccgac catgttttaa ggcggcaacg cctgttgtgc
     8461 cgctgcccat aaaagggtca aaaattaaat ctccgatatt tgaagccgat agtatgcagc
     8521 gttcaagtaa ggggagtggt ttttgtgtcg gatgtttgcc gaatgttttt tcggttttat
     8581 ttggtggagg aaatgtccaa acacatttca tctgtttgcc atcattttgt gttttcatgg
     8641 tttcataatg aaacgtgtgt ttagcttttt tatttttctt tgcctataag attgtttctg
     8701 tcgaatgggt aaagaaacgg caggacaaat tagggggagg attgggtttt tcccaagtaa
     8761 tattgttcaa aatatggtag ccgacggttt gcatcaggta gccgattaaa tagatattat
     8821 gaaatgtgcc gcaaacccaa attgtgccgt ttggttttaa taaggcgtaa cacagtcgga
     8881 gccattcttc gtaaaattcc aaatctgccg ccattccttt ggatttatcc cagttgccct
     8941 tattgacgga aaccatttgc ccgttttgac aactgaaacc gtcattggaa agaaagtaag
     9001 gcgggtctgc gaaaatcata tcaaaacagc cgtttggatg tttttccaat atcttccgca
     9061 tgacgttaag ggaattttcg ttgtagagga caaggtcgtc tgaaacgatt ttttcaaacg
     9121 actttgataa atggcagctt cttcagccgg tttgtcaaaa tcaatcataa attgccaata
     9181 attctccgac ttttttacga ctgctgccct ttgcggcaat attgcgttgt acttcaaccg
     9241 tttttaaaat ggcagaagaa taaatatttc tcgtaaattc agtatcatga tttgaaatca
     9301 aaacgccttg ggagttgggt gcaatttgtc gatttttttg taaagtccgc gaccaatgaa
     9361 ttcgatcata ttttgatcac gcaaaatttg caactgttgg cggattttgt ctttgatatg
     9421 gttgttttgg ggaaattgga tggatagttt gttttcaaat tcatacattt gcgacaatgt
     9481 gaattcttcg gtgagttggt cgatacattt cataacagcc aaaagccagt ctttgcgctc
     9541 cgcattttgg ttgcgtaaaa acaggttgga ttgccatttt ttcagaacgg tttcgggttc
     9601 gataatgcgg gaatcgtcta ttaagaatat ttttccgctt tcaggcaaag gggcgagatt
     9661 gatggaacac ataatgtggt gcggtcggtt tttaatgcct ttatttctgg gaataatcat
     9721 atccggcgtg atgaaatgtt tgggtacaag caccaattgc cgtatggagt aatccgcttt
     9781 tttatatgca agaaagaaaa agttggggtt ggtatctgac cggatgcgct ccaacatagt
     9841 gcgatatgca ccgtcaggca cgctgttgcc tatggttttt tgatttttac tctttaattc
     9901 atattgctcg tggcaatttg ggcaaaagag gtctgcaaca ggtttgttat tggcaaatct
     9961 ctgcatcggc ttgcttccgc aacaggggca atagccgttt ttttccagcc aagcctcgct
    10021 cattacacgg attttgtggg ttgctttatt ttgttgcttt cccaattggg tatcgaaaaa
    10081 taaattcata ttttggattt tgagattaat ttttagtaga ttaacaaaaa tcaggacaag
    10141 gagacgaagc cgcagacagc agggcaaggc ggggcaacgc cgtaccggtt taaagttaat
    10201 ccgctatatt tgaggtctgt cttgcagaca acccaatctg ccttaaaaag gccgtctgaa
    10261 accctgtttc caagtttcag acggccttta tccgtatggc taaaccttaa aagcggttta
    10321 gacgacgatg ttcaccagtc tgcccggtac gacgatgatt ttcttcgcag gcttgccttc
    10381 catgaatttc actgcgcctt cgttggcgag tgcggcggct tcgaggtcgg ctttggaggc
    10441 gtcggcggcg acggtgattt tgccgcgcag tttgccgttg acttgaacca tcacttcgat
    10501 ttcggatttg accagggcgg cttcgtcgac tgtcggccag cctgcttccc acagtttcgc
    10561 gccgttcaat tcgctccaca gggtttcgca gatgtgcggc acgatgggcc acaacaggcg
    10621 tacggcggct tccaatactt cttgggcgac ggcgcggcct tgttcgctgc cggtgtcggt
    10681 tttgtcgtat tggttgagca gttccatcac ggcggcgatg gcggtgttga actgctggcg
    10741 gcggccgtag tcgtcgctga ctttggcggt ggtggaatgc agtttgtggc gcaggtcttt
    10801 gagttcttta gacaaaccgt cttggttgcc tgcaaatgct ttgaccgcgc cgccttgctt
    10861 caggtattcg taaacggtac gccacagacg gcgcaggaag cggtgtgcac cttcgacgcc
    10921 gctgtcgctc cattcgaggg actgttcggg cggtgcggcg aacatcatga acaggcgggc
    10981 ggtgtccgcg ccgtaggcgt taatcagttc ttgcggatcg acgccgttgt ttttggattt
    11041 ggacattttt tccgtgccgc tgatgacgac gggcagtccg tcggctttga ggacggcgga
    11101 aacggggcgg cctttgtcat cgaaagtcag ctcgacatcg gcggggttga tccagtcttt
    11161 gccgcctttg tcgttttcgc ggtagtaggt ttcgcagacg accatacctt gcgtgagcag
    11221 gcgttcaaac ggttcgtcaa cattgaccaa accttcgtcg cgcatcagtt tggtgaagaa
    11281 gcgcgcgtac aggaggtgca aaatcgcgtg ttcgatgccg ccgatgtatt ggtcgaccgc
    11341 gccccagtat ttcgcggatt ctgccgatac catgccgtct gaaaacttgg gcgacatata
    11401 gcggaagaag taccagctcg actccatgaa ggtgtccatg gtgtcggttt cgcgtttcgc
    11461 cgcgccgccg cagcacgggc aggaagtttc gtaaaactcg ggcatttttg ccagcggcga
    11521 acccataccg tcgggtacga cgttttcagg caggacgacg ggcagttggt cggcagggac
    11581 gggaacgtct ccgcattttt cgcaatggac gatgggaatc gggcagcccc agtagcgttg
    11641 gcgcgaaatg ccccagtcgc gcaggcggta ttgggttttc ggttcgcccg caccttggct
    11701 ttgcagcttg gcggcaacgg catcaaatgc cgtctgaaaa tccaagccgt ccaagtcgcc
    11761 gctgttgacc aatacgccgt tttctttgtc gccgtaccat tcttgccatc ggtttgcgtc
    11821 gaatgcgttg tcgccgacgg caatgacttg ttttttcggc agattgtatt tggcggcaaa
    11881 ctcgaaatcg cgttcgtcgt gcgccggaac agccatcacc gcgccgtcgc cgtagcccca
    11941 caagacatag ttggcaatcc acacttccag cttgtcgccg ttgagcgggt tgacgacgta
    12001 gcggccggtc ggcacgcctt ttttctccat cgtcgccata tcggcttcgg caaccgaacc
    12061 ggctttgcat tcggcgataa atgcctgcaa ttcgggtttg tcggcggctg cggctgttgc
    12121 cagcggatgt tcggcggcaa cagcgacata agtcgcaccc atcagcgtgt cggggcgggt
    12181 ggtataaact tgcaggaatt tcgcgtaatc gccttccaaa ccttgcttgc tgtcgtctga
    12241 aacggcgaag cgcacggtca tgccgcgaga tttgccgatc cagttgcgct gcatggtttt
    12301 gacttgttcc ggccagtgtt ccagcttgtc caaatcgttg agcagctctt cggcgtaatc
    12361 cgtgattttg aagtaataca tcgggatttc gcgtttttcg atcaacgcgc ccgaacgcca
    12421 gccgcgtccg tcgatgactt gctcgttggc aaggacggtt tggtcgaccg ggtcccagtt
    12481 caccgtgccg tttttgcgat agacgatgcc tttttcaaac agcttggtaa acagccattg
    12541 ttcccagcgg tagtattcgg gtttgcaggt ggcgacttcg cgttcccagt caaccgcaaa
    12601 acccaggctt ttgagctggg ttttcatgta ttcgatgttg tcgtaggtcc aagcggcggg
    12661 ggcgacgttg tttttcatcg ccgcattttc cgccggcatg ccgaacgcgt cccaacccat
    12721 aggctgcata acgttgaagc cgtttaaaag tttgaagcgg ctcaatacgt cgccgatggt
    12781 gtagttgcgt acatgcccca tatgcagctt gccgctaggg taagggaaca tcgaaaggca
    12841 ataatatttg ggtttggaag cgtcttcgga gacgttggaa atacgggcgt cgtcccattt
    12901 tttctgcgcc gcaggctcga tggcggcggg ctggtaatgt tcttgcatgg tcattttgtt
    12961 tttcgcttga aaacgttagg aatttaaagg aggaattata acaggttgcc gatgtcggcg
    13021 aatcggcaaa ttgccggcag gatgcgtaaa ttcgcacgcg catcattccg tatgccgtac
    13081 aaatacaccg cgtttactga tacgcacgtt ttttatgcta atattacgaa ccaaaatcaa
    13141 acgtttaaaa ctctcctgat gcggctcttc cgaacaaaag gcagacgggc atcgggtaaa
    13201 aaaggattct gcatatgaaa atcaaacaaa tcgtcaaacc gagcttggca gtattggcgg
    13261 caggcattct gtctgcctgc gcaaccataa agcaacgtca aagccgacgg cacgaccgac
    13321 aatccggttt tcccggaacc ctattccgta acgctcgaca acaagcgcgg cacattcccg
    13381 acttatgacg aactggatca gatgcgcccc ggtctgacca aagacgacat ctacaaaatc
    13441 ccgggccgcc cgcattacga cgaaggtatg tacggcgtgc gcgaatggga ttacctgttc
    13501 cacttccaca ccccgggcgt gggtatcgac cctgaaaaca cttccggcgt ggaaggcgtt
    13561 actacctgcc aatacaaagt gattttcgat aaagacaaat ttgcccgcag cttctactgg
    13621 aaccccgtct tcccgaaaga tgccgtctgc ccgccgcccg cacccaaagc cgagccgcaa
    13681 gtcatcatcc gcgaaatcgt gccggcaaaa ccgaaacgta tccgccaata agccgccatg
    13741 ccgccccgcc tgctttcagg gatattgtgc tgcctgctga cgactgcccc cgtattcgca
    13801 caggggcagc cggatgcggt cagtgcctat attcaaaaga aaaaagtcat cgtcgacaca
    13861 tccaaggcgg aactctgttt tgccgacgac cgtcagtgcc accccgtcct catcggcact
    13921 gccacaccca aggggacgtt cggactgacg ctgaacagta ccgacaagcc cggttacggc
    13981 ggcgaagtca tcggtttcaa gcaggagggc gattttcttt tcgccctgca ccgcgtttgg
    14041 aatcagatac cgtccgaaag gcgaaacgaa cgcatcgcct ccccgtccgt gtccgacagg
    14101 attatgacca acggctgcat caacgtcagc gatgcggtgt acgaaaaact gcgtcattat
    14161 tttgtgttgg aagtgatttg aaacagacgg ataccgcacg tgccggtatc cgttttcaca
    14221 ttgccccgat gcctgaaaca gaccgtccgt cacgtcatgc cgcctgaaac cggcgcagat
    14281 gccgccaagc cttcagacgg cattgtccgc ccgctccgac cgaacaacaa ccatctttgg
    14341 gagaacctta tgaccgaaca aaaccgcatc ctctgccgcg aactgagcct gctggcattc
    14401 aaccgccgcg tgttggcaca ggcggaagac aaaaacgtcc cccttttgga acgcctgcgc
    14461 ttcctgtgca tcgtgtcgtc caatcttgac gagttttttg aagtccgcat ggcgtggctc
    14521 aagcgtgaaa acaagctgca cccccggcgc aggccggaca acggcaaaac gccgtctgaa
    14581 accatcgccg acgttaccga agcggcgcgc tccctgatac ggcaccagta cgacctgttc
    14641 aacaacgtcc ttcagcccga gttggcgcgg gaaagcatcc atttttaccg ccgccgcaat
    14701 tggacgggca cgcagaaaaa atggatcgaa gactatttcg accgcgaact gctgccgatc
    14761 ctgaccccca tcggactcga cccttcccac cctttcccgc gcccgctgaa caaatcgctc
    14821 aacttcgccg tagaactcga cggcacggac gcgttcggca ggccttcggg tatggcgatt
    14881 gtccaagcac cgcgcatcct gccgcgcgtt gttcccctgc cgtccgaact gtgtggcggc
    14941 ggacacggct tcgtcttcct gtcgtccatc ctccacgccc acgtcggcaa actcttcccg
    15001 ggcatgaacg tcaaaggctg ccaccagttc cgcctcacgc gcgacagcga cttgaccgtt
    15061 gacgaagaag acgtacaaaa cctccgcgcc gccattcaaa acgaactgca cgaccgcgaa
    15121 tacggcgacg gcgtgcggct cgaagtcgcc gacacctgtc ccgcctacat ccgcgacttt
    15181 ctgctcgcgc aattcaggct gaccgacgcc gaactctatc aggtcaaagg cccggtcaac
    15241 ctcgtccgcc tcaacgccgt ccccgaccta gtcaaccgcc ccgatttgaa atttccgccg
    15301 cacacgccgg gccgtctgaa agccttgggc aaaaacagcc cgatcttcga tttggtgcgc
    15361 caatcgccca tcctgctgca ccacccctac caatctttcg atcccgtggt cgatatgata
    15421 cgcgaagccg ccgccgatcc cgccgttctt gccgtcaaaa tgacgattta ccgcaccggc
    15481 acgcgctccg aactcgtccc cgccctgatg aaggcggcac tcgccggcaa acaagtaacc
    15541 gtcgtcgtcg aactgatggc gcgttttgac gaagccaaca acgtcaactg ggcgaagcag
    15601 ctcgaagagg cgggcgcgca cgtcgtgtac ggcgtgttcg gctacaaagt ccacgccaaa
    15661 atggcactgg tcatccgccg cgaagacggc gtgctcaagc gttacgccca cctcggcacg
    15721 ggcaactacc accaaggcac atcgcgcatc tacaccgact tcggcctcat taccgccgac
    15781 gaacaaatca ccgccgacgt gaacacatta tttatggaaa tcacaggctt gggcaaaccc
    15841 gggcggctga acaaactcta ccaaagtccg tttaccctgc acaaaatggt tatcggccgc
    15901 atcgctcgcg aaaccgaaca cgcaaaagcc ggcaaaccgg cgcggattac cgccaagatg
    15961 aattcgctca tcgaaccgac tgtcatagaa gccctgtatc gggcaagcgc ggcaggcgtg
    16021 caaatcgatt tgattgtgcg cggtatgtgt accttgcgcc cgggtgtaaa aggcttgtcc
    16081 gaaaacatcc gcgtccgctc catcgtcggc aggcagctcg aacacgcgcg cgtatattgt
    16141 ttccacaaca acggcgcgga cgacaccttt atttccagcg cggattggat ggggcgcaat
    16201 ttcttccgcc gcatcgaaac cgccaccccg attaccgcgc ccgaactcaa aaaacgcgtc
    16261 atccgcgaag gtttggaaat ggcactggca gacaataccc acgcgtggct gatgcagccc
    16321 gacggcggct atatccgcgc cgcgcccgcc gaaggcgaaa gcgaagccga cctgcaaaac
    16381 gatttatggg atttgctccg aggctgaccc gcacaagcac ttcccccata cgctcccaac
    16441 cccaatccgc cgcattccag acggcattgc ctttccggca gccgaacaaa accccgaatc
    16501 cgtctgcacg gttcggggca tatctccaat acgggcatcg tgttccggaa aaccgtcaaa
    16561 tccgcatcgg catcacaata tatttgaaat tcggattgtt cggcacggta aacagcgtcg
    16621 agcggttggc gtcgccgaag gcaagctgca tatcgtcgga atggatgttg cgcaacacgt
    16681 ccatcaaata gccgatattg aaaccgactt cgagttcgcc gccctgatag gcgatttcga
    16741 tttcttcgcg cgcttcttcc tgctcgttgt tgctgcacac gacgctcaac aggccgggtt
    16801 gcaggaacag gcgcgcgcca cggaattttt cgttggcaag aatggacaca cgttccagcg
    16861 cgcccaaaag ttcggcacgg gacaacacga aaatcttgtc gttgtccaaa ggaatcacgc
    16921 ggttgaaatc ggggaatttg ccgtcgatga ccttgctgac gatggtcgtg ccgttgcatt
    16981 ggaaacgcac ctgcttgtcc agcagctcga tttgaatcgg atcgtcgggg ttgttcaaca
    17041 gtttgaacag ttccagcacc gttttgcgcg gcaaaatcac ttcggcgcgc ggcaaatccg
    17101 catcaatcgc gcaggctgca taggcaaggc ggtgtccgtc ggtcgccaca aggcgcaact
    17161 ggctgccctc aacctgcatc agcagaccgt tgagataata gcggatgtcc tgcaccgcca
    17221 tgctgtactg cacttgcgac agcatggttt tgaaacgctc ctgccccagc gagaaagtcg
    17281 cgctgatgtc ctcgccgaca ttcatcatcg gaaaatcggc ggcaggcagg gtttgcaggg
    17341 caaaacgcga tttgcccgcc ttcagcgtca gacggttgtc gtcccaatcc agcgacacca
    17401 gcgcaccggc aggcagcgcg cgcaaaatgt cctgaaattt cttggcattg gtagtgatgc
    17461 ggaagtcgcc cgcacctccc tcgggacccg cggtgtcgat ttggatttcc aaatcggttg
    17521 ccaagagttt ggtctgaccg cccctgccct caatcagcac attggacagg atgggcaggg
    17581 tgtgtcggcg ttcgacgatg ccggtaacgg cttgcagcgg cttgagcagg ctgtcgcgct
    17641 cggcttgtaa aatcaacatt ttcgctcctt aaatcggttg gatttcctac ccttcggacg
    17701 gcatggtcaa tgtcatgccg tctgaaaggc gtgtccggtc agttttgaat cagaatcagc
    17761 agtttctcgt aatcctgcgc caactcgggg tcttcctcgc gcagtttcgc caccgccctg
    17821 atgccgtgca tgacggtcgt atggtcgcgt ccgccgaacg aatcgccgat agacggcagg
    17881 ctcaaagtgg tcaattcttt ggtcaggctc atagcaacct gacgcggacg ggcaatgttg
    17941 cgcgtgcgtt ttttgccgag tacgtcgctg attttgatgc ggtaatattt cgccaccgca
    18001 tcgatgatga tgtcggcggt gatgactttg tgcttctcgg caataatgtc ctgcaaagcg
    18061 gtacgcgcca aatcgatgtc gatgacggga cggttcataa agcggctgct cgctccgaca
    18121 cggttgaacg cgccttccag ttcgcgcacg ttggaacgga tcagattggc aatgaacagc
    18181 gcggcttcgt cttcgatact gatgcccgcc gcttccgcct ttttctgcaa aatggcgata
    18241 cgcatttcca attcgggcgg ctcgagttcc aaagtcagcc cccacgaaaa gcgggatttg
    18301 aggcggtcgt ccataccttc gattttggcg ggtaaaacat cgcaagtgag gatgagctgt
    18361 tttttctcat tgtgaaaatg gttgtacaga tagaaaaatt cttccatcgt acggtctttt
    18421 cctttgatga actgaatatc gtcgataatc agcaggtcgt attgcttgta ttgctgcttg
    18481 aacacgtcgt aggtattgtt gcgaaccgcc ttcataaagc tgcggatgta gtcgtccgaa
    18541 tgcatatagc gcactttggc atcgggacgg tttttcaaca gctcgttgcc gaccgcctgc
    18601 acaaggtggg ttttgcccaa acccgtgctg ccgtacagga agaacgggtt gtaactctgc
    18661 cccgggtttt ccgcaatcgc ctgcgccgca gccgccgcaa ggcggttgcc cttaccttct
    18721 accaacgtat caaacgtgta atccggagac aggttggttt gttcgtaacg cgcttcttcc
    18781 gcatcacgct gcgcatccgt ccgcgctttg gcaactgccg ccgattccgg ccgggaagca
    18841 ggcccggcag cctgacgcgg ctcgtgcggc aggtttttca tacgttccgc caaaatatcc
    18901 gccgccgttt tcgacgcagc gggtttgaca ggcttttcag acggcagctc gtccaacaga
    18961 acctcctgca cgggcatctc ctccgacccc gcgtgcaagg acggctcggc aggttcgaca
    19021 gcaccttcaa ccgccgccat ctcataacgc acgccttctc ccggtttgaa tacgaaggcg
    19081 ggacggccgg cagccaactc ttccctcacc gcttctattt ttccggcaaa ctggctcttg
    19141 agcatattgc aggcaaactg gttcttgccg tacaccaccc atacgccacc ctcctcacca
    19201 accgtaaggg gcgcaatcca ttgcgcaaac tgcccgtgag gcaacatatc gtgaagacgg
    19261 cggaggcaca gcggccaaaa ctctgctaat gtcatggata ggctcgaatc ggtaaaaatg
    19321 aaatcgaaaa caaagaaaat ataatatttt caaaaagaaa acaaatctgt tgaatacgcg
    19381 tcggttcaaa acgcagcgaa ccgattatac cgactcgccg atattttatc cacaacccgt
    19441 gcaaaaattt atccacagaa aggcggcaga aatccgcagg caatcgggca atcctcctgc
    19501 aaagtttcta tattgattga caaaagcggc aaattggagt gtaattcacg gtttaattat
    19561 ctacccattc tattttagga accatcatga aacgcactta tcaaccttcc gttaccaaac
    19621 gcaaacgcac ccacggcttc ctcgtgcgct ccaaaacacg cggcggacgc gcagtattgg
    19681 ccgcacgccg tgccaaaggc cgcaaacgcc tggcagtgta attttggact accgcttcgg
    19741 aaggcagtac cgcttgttaa aaacggatga tttttcatcc gtttttgcat tcagaaaccg
    19801 ccgcagccgc gacctgctgc aagtttcgcg ctcaaacggc aacgggctgg accatccccg
    19861 catcggtctg gtggtcggca aaaaaaccgc caaacgcgcc aacgagcgga actatatgaa
    19921 acgcgtcatc cgcgactggt tcagattgaa caaaaaccgg ctgccgcctc aggatttcgt
    19981 cgtgcgcgtc cgccggaaat tcgacagggc taccgcaaaa caggcaaggg cggaactggc
    20041 acaactcatg ttcggcaacc ccgcaaccgg atgcgggaaa caggtatgat cagaacggta
    20101 ctctgcacgc aaggttcaga cggcaacggg tttcccatac aaggaacatc ccgatgaact
    20161 tcctattgtc caaactgctg ctggggctga taaggtttta ccaatactgc atcagcccgc
    20221 tgattccgcc gcgctgccgt tatacgccga cctgttcgca atacgcggtc gaagcggtca
    20281 aaaaatacgg cgcattcaaa ggcctccggc tcgccatcaa gcgcattgca cgctgccacc
    20341 ctttcggcgg acacggacac gaccccgttc cctgacccga cgcaatattc aaattgcacg
    20401 ctttcctttt atttcccatc ggtttctata taatgccgtc tgaaccttcg ggctggcggc
    20461 acggccgtcg ggtatgaagc ccgcccttat tccccgtcta tcggaacacg caacctgcgg
    20521 catttccgac cattcaggaa actctatatg gattttaaaa gactcacggc gtttttcgcc
    20581 atcgcgctgg tgattatgat cggctgggaa aaaatgttcc ccaccccgaa acccgtcccc
    20641 gcgccccaac aggcggcaca aaaacaggca gcaaccgctt ccgccgaagc cgcgctcgcg
    20701 cccgcaacgc cgattaccgt aacgaccgac acggttcaag ccgttattga tgaaaaaagt
    20761 ggcgacctgc gccggctgac cctgctcaaa tacaaagcaa ccggcgacga aaacaaaccg
    20821 ttcgtcctgt ttggcgacgg caaagaatac acctacgtcg cccaatccga acttttggac
    20881 gcgcagggca acaacattct gaaaggcatc ggctttagcg caccgaaaaa acagtacacc
    20941 ctcaacggcg acacagtcga agtccgcctg agcgcgcccg aaaccaacgg actgaaaatc
    21001 gacaaagtct atacctttac caaagacagc tatctggtca acgtccgctt cgacatcgcc
    21061 aacggcagcg gtcaaaccgc caacctgagc gcggactacc gcatcgtccg cgaccacagc
    21121 gaacccgagg gtcaaggcta ctttacccac tcttacgtcg gccctgttgt ttatacccct
    21181 gaaggcaact tccaaaaagt cagcttctcc gacttggacg acgatgcgaa atccggcaaa
    21241 tccgaggccg aatacatccg caaaaccccg accggttggc tcggcatgat tgaacaccac
    21301 ttcatgtcca cctggatcct ccaacctaaa ggcggccaaa gcgtttgcgc cgcaggcgac
    21361 tgccacatcg acatcaaacg ccgcagcgac aagctgtaca gcgcaagcgt cagcgtgcct
    21421 ttagccgcta tccaagccgg cgccaaagcc gaaactgccg tcaacctgta tgccggtccg
    21481 caaaccacat ccgttatcgc aaacatcgcc gacaacctgc aactggcaaa agactacggt
    21541 aaagtacact ggttcgcatc gccgctcttc tggctcctga accaactgca caacattatc
    21601 ggcaactggg gctgggcaat cgtcgttttg accatcatcg tcaaagccgt actgtatcca
    21661 ttgaccaacg cctcctaccg ttcgatggcg aaaatgcgtg ccgccgcacc caaactgcag
    21721 accatcaaag aaaaatacgg cgacgaccgt atggcgcaac agcaagcgat gatgcagctt
    21781 tacaaagacg agaaaatcaa cccgctgggc ggctgtctgc ctatgctgtt gcaaatcccc
    21841 gtcttcatcg gcttgtactg ggcattgttc gcctccgtag aattgcgcca ggcaccttgg
    21901 ctgggctgga ttaccgacct cagccgcgcc gacccctact acatcctgcc catcattatg
    21961 gcggcaacga tgttcgccca aacctatctg aacccgccgc cgaccgaccc gatgcaggcg
    22021 aaaatgatga aaatcatgcc gttggttttc tccgtcatgt tcttcttctt ccctgccggt
    22081 ttggttctct actgggtggt caacaacctc ctgaccatcg cccagcagtg gcacatcaac
    22141 cgcagcatcg aaaaacaacg cgcccaaggc gaagtcgttt cctaaatgcc gcagcatgaa
    22201 aaatgccgtc tgaaacctgt tcagacggca tttttattgt tcacccccta tcggggcgga
    22261 aatcttcaac ccgcatacat cacaaaaatc gtcgggcgtt ttttcagatt gggcatttcc
    22321 ttcatcctgc gccaatccgc aaccgtttta ctgaccacca actgagtcgg caacgtcaaa
    22381 tccgtagccg tacacaagcg cgtttcagga tgcaggtttt ccaccgcatc ggcaagcagc
    22441 gcatcattgc ggtaaggcgt ttcaataaaa atctgcgtct cgccgcactg ccgcgaacgt
    22501 tgctccaaag cattcaaact ctgaatgcgc tcattttttt cagacggcag ataaccatta
    22561 aacgcaaagt tctgcccatt cgcacccgaa gccatcagcg ccagcagcag gctggacggg
    22621 ccgaccaaag gccgtacttc aaaaccgtgt ttatgcgcca atgccaccaa attcgcgccc
    22681 ggatcggcaa cagccgggca acccgcctcg ctgacaatcc ccatactgcg cccttcttgc
    22741 aaaggtttca gcaattccgg caaagtcttc aaatccgtgt gttcattcaa cgtttgcaga
    22801 ttcagttcgc gtatcggcgt cgttatcccc aaatgcttca aatgcgcacg cgccgttttt
    22861 tccgcttcca cgacaaaatc cgtcagcccg acaaccgccc gttgttcatg ctgcaacagg
    22921 cacggcgtgt caggcgtacc caaaggcgta ggaatcaaat acaaaacagg agacatcatt
    22981 ccctcactca tcggttaaaa atgccgtccg aacccttcag acggcataaa cgggcagtta
    23041 caaaacatcc acgccctcgt ttttcaaaaa atcgaccaga cggaaaaccg gcagaccaat
    23101 caaggcattc ggatcggtac tttcaatccg ttcgatcaac agcgcgccca agccctcgct
    23161 tttcgccgca cacgaacaat aaaccgcatc cggctcgcgc tccaaatagc ggaggatgtg
    23221 cagctcgtcc aactgcctca tcacgaccac cgtcttatcg atatgccgat gcatcctgcc
    23281 cgtaaccgta tttagcagaa cgaccgcact gtaaaactca atctccctgc cgctcaaatg
    23341 catcaacatc ttttgcgcgt tggcaaggtt catcggcttg ccccactgcc tgccgtcgca
    23401 ccacgccacc tggtccgcac cgacaatcaa cgccccgggg aaacgcccgg tcaacgaccg
    23461 cgccttaccc tcggcaaggc gcaatgccgt ctgaggggcg gattccccca acatcggcgt
    23521 ttcgtcaaaa tcgggggatg ccgcctgaaa ggcaatgccg agcctttcca tctgttcgcg
    23581 gcggaaaacc gaactcgtac ccaaaaccaa aggcagttcc aaacccatcc catcctcctt
    23641 accgttgaaa acacgcccga aggggcggta aaatccagcc atgcgccgaa acacggacgc
    23701 ccgccttcgg cgtaccgcaa catttttctt aaaaatattg acgttagaac atctaaatta
    23761 tatcataccc cgtttatgtc agaccctaat ttgattgacc cggaaatttt tgccgccgaa
    23821 aggcagaacc tgcaaggcag ttttctgctg gaagaattgg acgagcgagt cagtttgcac
    23881 gattatcccg ccgacaggcg gaccaaaata tcgtttacac tgaccggcgg tcgcgaccgg
    23941 ctgcaacgcc tgttcctcga cctgaacgtc aaagccgata tgcccctgat ttgccagaga
    24001 tgtatcaaac ccatgccgtt catgctcgat gaaagcagcc gtatcgtcct gttttccgac
    24061 gaagagtcct tggacgaatc catgcttgcc gacgaagaac tcgaaggcat actgattgaa
    24121 aaagaactcg acgtgcgcgc attggtagaa gaccaaatcc tgatgtccct gcccttttcg
    24181 ccgcgacacg gacactgcgg caataccctt ccggaatccg ccaaccaaga caaacccaac
    24241 ccctttgctg ttttggcggg tttgaaaagc agttaattag gacacagttt atttatctag
    24301 gagcttgaaa tggccgttca acaaaacaaa aaatcccctt ccaaacgcgg tatgcaccgt
    24361 tcgcacgacg cactgaccgc gcccgcactg tttgtcgaca gcacaaccgg cgaagtacac
    24421 cgcccgcacc acatctcccc caacggtatg taccgcggcc gcaaagtggt caaagccaaa
    24481 ggcgaataat ccctattcga ctgactgaaa aagccagaac attgccatgc aattactggc
    24541 tttttttaca ttggacgcac catccgtcca aactttcgcc atacgtcaac acacaggggc
    24601 aaagcgttcc gtataatacc ccgtgaaaat attccaaaag ccccaaccac caaggaaatt
    24661 ccgatgaaac agaaaatctg gtacacctac gatgacatcc accgcgtcat caaagcattg
    24721 gcagaaaaaa tccggaacgc cggcgtcaaa tacgatgcca tgatcgctat cggcggcggc
    24781 ggctttattc cggcacgcat gctgcgctgt tttctggaaa ttccgattta tgccgtaacc
    24841 accgcctatt acgacagcga caacgaagga caagttaccg aagaagtcaa aaaagtccaa
    24901 tggctagacc ccgtccccga agtcctgcgg ggcaaaaacg tgctcgtcgt cgatgaagtg
    24961 gacgacagcc gcgtaaccat ggagttctgc ctgaaagaac tgctcaagga agacttcgac
    25021 actgtcggcg tcgccgtcct gcacgaaaaa atcaaagcca aagcaggcaa aatccccgaa
    25081 ggcattccct atttcagcgg cattaccgta gaagactggt ggatcaacta tccgtgggac
    25141 gcactcgaca tcgacgaaca caaccgcctt gccgaagccg gccgaggcta accctttcag
    25201 acggcatatt ttccgaaccg atgccgtctg aagcccgcac gacccctgcc gcagaccgaa
    25261 aatctaccgg agaaacccct atgattacat tggccgtaga tgccatgggc ggcgaccaag
    25321 gacttgccgt taccgtaccc ggcgcaaccg cattcctcca agcacacccc gatgtccgcc
    25381 tgattatgac cggcgacgaa acgcagctcc gccaagccct gaacgcggca ggcgcgccga
    25441 tggaacgcat cgacatctgc cataccaccc aggttgtcgg catggatgaa tcccctcagt
    25501 ccgccctaaa gaacaaaaaa tactcctcca tgcgcgttgc catcaaccaa gttaaagaag
    25561 gcaaagccca agccgccgta tccgcaggca acacgggcgc gctcatggca accgcacgtt
    25621 tcgtcctcaa gaccataccc ggcatcgaac gccccgccat cgccaaattc cttccttccg
    25681 acaccgacca cgttaccctc gccctcgacc tcggcgcaaa cgtcgactgc acacccgaac
    25741 agcttgccca atttgccgtc atcggcagcg aactcgtcca cgcactccat cctcaaaaag
    25801 gacagccgcg cgtcgggctg gtcaacgtcg gcacagaaga catcaaaggt acggataccg
    25861 tcaaacaaac ctacaaactg ctgcaaaaca gcaaactcaa ctttatcggc aacatcgaaa
    25921 gcaacggcat cctgtacggc gaagcagatg tcgtcgtcgc cgacggcttt gtcggcaacg
    25981 tcatgctcaa aaccatcgaa ggcgcagtca aattcatgag cggagccatc cgccgcgaat
    26041 tccagagcaa cctgttcaac aaacttgccg ccgttgccgc cctacccgcc ctcaaaggct
    26101 tgaaaaacaa actcgatccg cgcaaattca acggggccat cctgctcggg ctgcgcggca
    26161 tcgtcatcaa aagccacggc ggcacagaca aaaccggttt ccgctacgca ctcgaagaag
    26221 cctaccacga agccaagtcc gccggccttt ccaaaatcga acaaggcgtt gccgaacaac
    26281 tcgccgcact ggaagccgcc caaaacgaaa ccgccgccag tctgtaacac gcacgatgcc
    26341 gtctgaacgc ccccgcccct ttcagacggc atccggcacc cgtccaaacc tgcgggcgcg
    26401 gacggcgatg cgcctgtccg gcacttccca aatacttccc tgtaaaataa ggagtatttg
    26461 aaaaatgaag acattagaaa aacggatgaa agctctagac aaacggatga tgaagttcgg
    26521 aaaatccctt gaaggcaggc ttgatgcccg tctgattgaa tccgcattgg attatattca
    26581 ttattcggaa cgttttttgg cttttgacat cctgtgtact tatatcgaag atttcgatat
    26641 ccggctgacg gaacaagaat cccgggaaat ttcttttatc aacaaggaat ttgagataga
    26701 aagcacgtcc gattaaccaa taaagccaat gatttgataa gaatgaaaac atcgacggtc
    26761 gtttttggcg gattttttat ggcagacaac ggggagcgaa tccaaatccc cgttttggaa
    26821 aatcctgaca ttagggaaat caatcgcttt ttttccgtat caaattttga gaaaaaagcc
    26881 ggcgtccttg ttttcagaat catccccgag ccggaatttg gcaataccga attaactgtc
    26941 tattttgaaa aaggatatta tagtggatta acaaaaaccg gtacggcgtt gccccgcctt
    27001 ggctcaaaga gaacgattcc ctaaggtgcc caagcaccaa gtgaatcggt tccgtactat
    27061 ccgtactgtc cgcggcttcg ccgccttgtc ctgatttttg ttaatccact atatttgcca
    27121 ataattcaaa caattttaga aggcggggat attaaagtaa aaaaccttaa aacagaaaat
    27181 tacagcggga aggcaaggga aatcttggga gattcctacc ctattgaaca tatctctaaa
    27241 aatatttcta caattcaaaa tatcatttcc gaatttatca gacaaaaaca aacaccggca
    27301 ccaaataatt tgatggaaac ccaacaatca agatgccgtc tgaagcagct tgggctttca
    27361 gacggcattt ccttcactta aaacagcgta tcggcaaccc cgccccgccc gtccacggca
    27421 atccgcatct gaaaaccatc tgtatgccaa accacacccc catccctgtt tccgtcattt
    27481 gcaccctgcc cgtattgggc aatcatcggt ttttcgctta caatagccga atctgaacca
    27541 actctctaaa aaggccgttc ccatgcagta tgcaaaaatt tccggcacgg gcagctatct
    27601 tcctgccaac cgcgtcagca atgacgacct tgcccaaaag gtagatacct ctgacgagtg
    27661 gattaccgcg cgtacgggca tcaaattccg ccatattgca gccgaaaacg aaaaaaccag
    27721 cgatcttgca gccgaagcgg cgcggcgcgc tttggcagat gccaaattaa atataaacga
    27781 cattgatttg attatcgtgg cgacggcaac gccggatatg cagtttcctt ctaccgccac
    27841 catcgtgcag caaaaattgg gcatcaccaa cggctgcccc gcgtttgacg tgcaggcggt
    27901 gtgcgcaggc tttatgtacg cgctgactac ggcaaatgcc tacattaaaa gcggtatggc
    27961 gaaaaacgcg ctggtcatcg gcgcggaaac cttcagccgc atcgtcgatt ggaacgaccg
    28021 cacaacctgc gtgctgttcg gcgacggcgc gggcgcggtg gttttaagcg cgtccgacaa
    28081 accgggcatc atccacagca aactcaaagc cgacggcaat tatctgaaac tcttaaacgt
    28141 ccccgggcaa atcgcctgcg gcaaagtttc cggttcgccg tacatttcga tggacggtcc
    28201 cggcgtgttc aagtttgccg tcaaaatgct gtccaaaatc gccgatgacg ttatcgaaga
    28261 agcaggttac accgccgaac aaatcgactg gattgttccc catcaggcaa accgccgcat
    28321 tatcgaatcg accgcgaaac atttgggttt gagtatggac aaagtcgtcc tgaccgtcca
    28381 agaccacggc aacacgtccg ccgcatccat tccgctggct ttggacgcgg gcatccgcag
    28441 cggacaaatc aaacgcggtc aaaacctgct gctcgaaggc ataggcggcg gtttcgcgtg
    28501 gggcgcggtg ctgttgcaat attgaaaccg gtgccgtctg aaacaggctt tcagacggca
    28561 tttcccatat catgaagcgg caggctttct tcaaaccgat ggcgtgtgcg gcatttctgt
    28621 ccgccgtttc gctgcgcctc cccgtattgg gcgcgtgtta cgcaatattg tccctctatg
    28681 cgtttgcact ttacggcatc gacaaacggc gtgccgtgcg gggaaaacgc cgcattcccg
    28741 aacaccgcct gctcctgcct gccttgttcg gcggttggac gggcgcatac ttgggtagta
    28801 ggatgttcag gcataaaacg gcgaaaaagc gttttgttgt gctgttccgt ctgactgttt
    28861 cgggcaatgt cctggcgacc tgcatcctga ttgattattt cgttccgccc gaactttttg
    28921 taaaactcgg gcaacatctc tgacaatcca acccaacaaa ggaatactat gtctttcgca
    28981 ttcttcttcc ccggacaagg ttcccaaagc ctcggcatga tgaacggctt tgccgaacac
    29041 gccatcgtca aaaacacatt tgacgaagcc tccgccatat tggggcagga cttgtgggcg
    29101 atgattaacg gcagcgatgc cgaaatcatc ggtcaaacag tcaacaccca gcccattatg
    29161 ctcgccgccg gcgttgccgt ttaccgcgcc tatttggaag tgggcggcaa aacgcctgcc
    29221 gccgttgccg gacacagcct cggcgaatac accgcgcttg tcgccgccga agcattggat
    29281 tttgccgatg ccgtcaaact cgtccgcctg cgcgccgaac tgatgcagtc cgccgtaccg
    29341 caaggcgtag gcgcaatggc ggccattttg ggtttggaag atgaacaggt caggcagatt
    29401 tgtgccgaat ccgcacaggg cgaagtggtc gaagccgtca acttcaactc gcccggacaa
    29461 gtcgtgattg caggcaacgc cgccgccgtc ggacgcacca tggctgccgc caaagaagcc
    29521 ggagccaaac gcgccctgcc gctgccggta tccgttcctt cccattgcag cctgatgaaa
    29581 cccgccgccg acaaacttgc cgaagcactc aaaaccgttg aaatcaagca gccgcaaatc
    29641 cgtgtcatcc acaacgccga cgttgccgcc tacgatgatg ccggcaaaat caaagacgcg
    29701 ctcgtccgcc agctttacag ccccgtgcgc tggacggaaa ccgtcaacgc ccttgtttca
    29761 gacggcattg ccgaatccgc cgaatgcggt ccgggcaaag tgttggcggg cttggcaaaa
    29821 cgcatcaaca aggccgccgc ttgcagcgca ctgaccgatg ccggacagat taccgccttt
    29881 atcgaagcgc actgacttcg ttccgcaaaa agcagcctgc cctcttcagg ctgcttttca
    29941 cgtccgaacg gcaaacagcc gcatatttgc gctataattc atccctacca aaccaccgac
    30001 agcggctgcc gttgcagttc ccgccctacc gatatgatag aaaaactgac tttcggactg
    30061 tttaaaaaag aagacgcgcg cagctttatg cgcctgatgg cgtacgtccg cccctacaaa
    30121 atccgcatcg ttgccgccct gattgccatt ttcggcgttg ccgccaccga aagctacctc
    30181 gccgccttca tcgcccccct gatcaaccac ggcttttccg cacccgccgc gccgcccgag
    30241 ctgtccgccg ccggcatcat ttccaccctg caaaactggc gcgaacagtt tacctatatg
    30301 gtttggggga cggaaaacaa aatctggacc gtccccctct tcctcatcat cctcgtcgtc
    30361 atccgtggca tctgccgctt taccagcacc tatctgatga cttgggtctc cgtgatgacc
    30421 atcagcaaaa tccgcaaaga tatgtttgcc aaaatgctga ccctttcctc ccgctaccat
    30481 caggaaacgc cgtccggcac cgtactgatg aatatgctca acctgaccga acagtcggtc
    30541 agcaacgcca gcgacatctt caccgtcctc acgcgcgaca cgatgatcgt taccggcctg
    30601 accatcgtcc tgctttacct caactggcag ctcagcctca tcgtcgtcct gatgttcccc
    30661 ctgctctccc tgctctcgcg ctactaccgc gaccgtctga aacacgtcat ttccgactcg
    30721 caaaaaagca taggcacgat gaacaacgtg attgccgaaa cccatcaggg acaccgcgtc
    30781 gtcaagctgt tcaacgggca ggcgcaggcg gcaaaccggt tcgacgcggt caaccgcacc
    30841 atcgtccgcc tcagcaaaaa aatcacgcag gcaacggcgg cacattcccc gttcagcgaa
    30901 ctgattgcct ccatcgccct cgccgtcgtc atcttcatcg ccctgtggca aagccaaaac
    30961 ggctacacca ccatcggcga atttatggca ttcatcgtcg cgatgctgca aatgtacgcc
    31021 cccatcaaaa gccttgccaa catcagcatc cctatgcaga cgatgttcct cgccgccgac
    31081 ggcgtatgtg catttctcga caccccgccc gaacaggaca aaggcacgct cgcgccgcag
    31141 cgtgtcgaag ggcgcatcag cttccgcaac gtcgatgtcg aataccgttc agacggcatc
    31201 aaagccctcg acaacttcaa cctcgacatc agacaaggcg aacgcgtcgc cctggtcgga
    31261 cgttccggca gtggcaaatc caccgtcgtc aaccttctgc cccgctttgt cgaaccgtct
    31321 gccggcaaca tctgcataga cggtatcgac atcgccgaca tcaaactcga ctgcctgcgc
    31381 gcccaattcg ccctcgtctc ccaagacgta ttcctgtttg acgacaccct gtttgaaaac
    31441 gtccgataca gccgtcccga cgctggcgaa tccgaagtcc tgtccgccct ccaagccgcc
    31501 aacctgcaaa gcctgatcga cgcatccccg ctcggactgc accagcccat cggatcgaac
    31561 ggcagcaact tatccggcgg acagcggcaa cgcgtcgcca ttgcccgcgc cattttgaaa
    31621 gacgcgccga tattattatt ggacgaagcc accagcgcat tggacaacga atccgaacgc
    31681 ctcgtccaac aagcgctcga acgcctgatg gaaaaccgca ccggcatcat cgtcgcccac
    31741 cgcctgacca ccgtcgaatc cgccgaccgc atcatcgtca tggatggcgg caaaatcatc
    31801 gaacaaggca cacacgatca actcatgttc caaaacggct actacaccat gctgaggaac
    31861 atctccggaa aagataccgc cgccgttcag acggcataaa caaaatgccg tccgaaatgg
    31921 tataatcgcc ccgacccctt cagacggcac tatatccgcc gacccatccg attatcttca
    31981 accaccgtaa aacccattat gacccaagac aaaatcctca tcctcgactt cggttcgcaa
    32041 gttacccggc tgatcgcccg ccgcgtgcgc gaagcacacg tttactgcga gctgcattcc
    32101 ttcgatatgc ctttggacga aatcaaagcc ttcaacccca aaggcatcat cctttccggc
    32161 ggccctaatt ctgtttacga atccgactat caagccgata ccggtatttt tgatttgggc
    32221 attccggttt tgggcatctg ctacggcatg cagtttatgg cgcaccactt gggtggcgaa
    32281 gtgcagcccg gcaaccagcg cgaattcggt tacgcgcaag tcaaaaccat cgacagcgga
    32341 ctgacacgcg gcattcaaga cgacgcgccc aacacactcg acgtatggat gagccacggc
    32401 gacaaagtgt ccaaactgcc cgacggtttc gccgtcatcg gcgacactcc gtcctgcccg
    32461 attgccatga tggaaaacac cgaaaaacaa ttctatggca tccagttcca ccccgaagtt
    32521 acccacacca aacaaggccg cgccctgttg aaccgctttg tcttggatat ttgcggcgcg
    32581 caaccgggct ggacgatgcc caactacatc gaagaagccg ttgccaaaat ccgcgaacaa
    32641 gtcggcagcg acgaagtgat tttaggtctg tccggcggcg tggactcttc cgtagccgcc
    32701 gcgctgattc accgcgccat cggcgaccaa ctgacctgcg tgttcgtcga tcacggtttg
    32761 ttgcgcctga acgaaggcaa aatggtgatg gatatgttcg cccgcaactt gggtgtgaaa
    32821 gtgatacacg tcgatgccga agggcagttt atggcgaaac tcgccggcgt gaccgaccct
    32881 gagaaaaaac gcaaaatcat cggcgcggaa tttatcgaag tatttgatgc cgaagagaaa
    32941 aaactcacca acgccaaatg gctggcgcaa ggcacgattt accccgacgt aatcgaatcc
    33001 gccggtgcga aaaccaaaaa agcccacgcc atcaaatccc accacaacgt cggcggcctg
    33061 cctgaaaata tgaagctcaa actgcttgag cccttgcgcg acttgttcaa agacgaagtg
    33121 cgcgagttgg gcgtggcttt gggcctgccg cgcgaaatgg tgtaccgcca ccccttcccg
    33181 ggccccggtt tgggtgtgcg catcttgggc gaagtgaaaa aagaatacgc cgacttgctg
    33241 cgtcaggcgg acgatatttt catccaagaa ttacgcaata ctaccgacga aaacggcacg
    33301 tcttggtatg acctgaccag ccaggcattt gccgtgttcc tgcccgtcaa atccgtcggc
    33361 gtgatgggcg acggccgcac ttactactac gtcgtcgcac tgcgcgcagt catcaccagc
    33421 gactttatga ctgcacactg ggcagagctg ccatactcac tgctcggccg cgtgtccaac
    33481 cgcatcatca acgaagtcaa aggcatcaac cgcgtggtgt acgatgtcag cggcaaaccg
    33541 cccgccacca tcgagtggga ataaacagca aacagggctg caccgtccgg cgcagtcctt
    33601 cgattatcgg aaaaaaggaa aaaatatgag cacacaagat ttaagcggca aaatcgcttt
    33661 ggtaaccggc gcatcgcgcg gcatcggcgc ggcaattgcc gacacactgg cggcagccgg
    33721 tgccaaaatc atcggtacgg caaccggcga gagcggtgcg gcggcgatta gcaaacgtct
    33781 ggcacaatgg ggcggcgaag gccgcgtatt aaattccgcc gaacccgaaa ccgtcgaaaa
    33841 cctgattgcc gacatcgaaa aaacgttcgg caaactcgac attctggtca acaacgccgg
    33901 catcacccgc gacaacctgt tgatgcgtat gaaagaagaa gagtgggacg acatcatgca
    33961 ggtcaacctc aaatccgtgt tccgcgcttc taaagccgtc ttgcgcggca tgatgaagca
    34021 gcgcgccggc cgcatcatca acatcacctc cgtcgtcggc gtgatgggca atgccgggca
    34081 aaccaattat gccgccgcaa aagcgggctt gatcggtttt gccaaatcta tggcgcgcga
    34141 agtcggcagc cggggcatta ccgtcaactg cgtcgccccg ggctttatcg ataccgatat
    34201 gacccgcgcc ctgcccgaag aaacccgcca aacatttacc gcccaaaccg ccttgggcag
    34261 attcggcgac gcgcaagaca tcgccgatgc ggttctgttc ctcgcttccg atcaggcgaa
    34321 atacatcacc ggtcaaacgc tgcacgtcaa cggcggtatg ctgatgccct aacggacagg
    34381 cttttcaacc atgccgtctg aagccctttc agacggcatt tgcattccca aacaaaatga
    34441 acactcacca ccacctgccc tgcccctgcg gttcaggcac aagctatgcc ggctgctgcc
    34501 gaccgcttca tctgcgccaa atcctgccgc ccaccgccga agccctcatg cgttcgcggt
    34561 acggcgcgta tgtgctgcac cttatcgact acatcatcgc tactaccgtt ccggcgcaac
    34621 aaacattcct cgatgccgcc gaactgatgc agtggagcag agaagccgaa tggctgggct
    34681 tgaatgtcat cgcacaccgc aacctcggca aacagcacgc ccaagtcgaa tttgaagcct
    34741 acttccaaga cggcggaaac cgaacagtgc atcacgagct gtcggcattc gtgaaaatag
    34801 cggagcaatg gtatttcatc gatccggctg ttccgctgcc taccatgaaa cagccctgca
    34861 tttgcggttc gggcaaaaaa ttcaaagcct gctgcggcaa atatctgcaa cctgtcgcat
    34921 aaaaatgccg tctgaacgtt cagacggcat tttcaacgcg caaaaaaaac cattcatacc
    34981 aagggtaagt atgaatggtc aatacattgc gggaaaacat cttacttgct gcactgccga
    35041 aaagggagaa acggcagcgt taaccagcaa aagcattgta cctttattaa tgttaaaaaa
    35101 cattaatttt gaaaatatat taacgaatat atctggaacc tactggtttt cccgtatcca
    35161 cccgacccag cgttcaaaca gcttcggttc gagcgcggcg acgaccgagc gtttgaacac
    35221 gtgttcacca ctccaaaacc cgtcgccttc caaagtcgtc agcctgccgc ccgcctcctc
    35281 gaaaatcaac gcacccgcag cataatccca cagcttctgc ccgccgtgga cataaacatc
    35341 ataacgcccg cacgccagat aacaccagtc cagcgtgctg ctgcccatac tgcgtatcgt
    35401 cccgaaaggc gcgagcgtac tcatgcggct ggaaagtttg cccgaacgca gatatttgat
    35461 ttccacgccc gcaatcgcct cattgagttt tttatccacg agacgcaaag gcaggcgtgt
    35521 cccattaaga aacgcaccct gcccgcgttc ggcataaaaa cattcgccgc tgaccggatt
    35581 gtagattacg cccaattcgg cgcgcccgtt gcggacaaac gccaccgaca cggcaaaatg
    35641 cggcagcccg ttgacaaaat tgttcgtccc gtctatcgga tcgacaatcc acagcccctt
    35701 ttcccccgaa tattgttccc acaaagccga ctgttcctgc cgcgacattt cctcgcccaa
    35761 catcgggcaa tcgatcagaa gcggcaacgc ggcggcaaat gccgtctgcg cggcaatgtc
    35821 cgcctcgctc aacatcgaac cgtcttcctt gcggcgggac ggcgtattca aaaaacgcgg
    35881 cataatttcg gtttgcgcga tatggcgcac gactttctgc aaacggtgta acacttccta
    35941 ctgtcctcat attttgaact tgcggcgcgc gaacgtataa tgtccgtttc catcacgccg
    36001 ctgcgacgga ttataaccgt ccgaaccgcc aaaaactatg cccagattct acctgcccga
    36061 aaacctttcc gtcggacaaa ccgtcgacct tcccgacaac atcgtccgcc acctcaacgt
    36121 cctgcgcgtc cgccccaacg aaaacatcac cctcttcgac ggcaaaggca aggcacatac
    36181 cgcacggctg accgttttgg aaaaacaccg cgccgaagcc gaaatcctgc acgaagacac
    36241 aaccgacaac gagtccccgc tcaacatcac gctcatccaa tccatttcct ccggcgatcg
    36301 catggatttc accctgcaaa aaagcgtcga actcggcgta accgccatac agcccgtcat
    36361 cagcgaacgc tgcatcgtcc gcctcgacgg agaacgcgcc gccaaacgcc tcgcacgctg
    36421 gcaggaaatc gtcatctccg cgtgcgaaca aagcggcagg aacaccgttc cccccgtact
    36481 gcccatcatc ggctaccgtg aagcactcga caaaatgccg tctgaaagca ccaggctgat
    36541 tatgagcatc aaccgcgcct gcaaactcgg cgacatccgc cacccgtccg gcgcaatcgt
    36601 ctttatggtc gggcccgaag gcggctggac agaacaggaa gaacaacagg catttgaagc
    36661 cggctttcag gcggttacac tcggaaaacg gattttacgc acagaaaccg ccccactcgc
    36721 cgccatcgcc gcgatgcaga cgctttgggg cgattttacc tagcagatga aaaaacacgg
    36781 gattttatcc gaaaaagcaa caatcccccc gccgtcattc ccgcgaaagc gggaatctag
    36841 aaatttaacg ttgcggtgat ttatcggaaa tgactgaaac ccaacgaacc ggattcccac
    36901 tttcgcggga atgacgaagt ggaagttacc cgaaacttaa aacaagcgaa accgaacgga
    36961 ctggattccc gcctgcgcgg gaatgacagt gtgtccattt ctgattttaa tcccctatat
    37021 tttacacaaa ctatttgaac aatatgaccc gcctgccgta aactttctca agctccgcct
    37081 gcctttgact ctccattctt ttcttctttt ccctaccgaa tttacccaaa gcacgcttca
    37141 agtcaaacat cgccttcaac gaacggcggt gtcttcttcc ttgttcccta tctttttcca
    37201 aatcgctacc caacatactg ttttgactga gaaacttggc ataatgcaat tcttgggtac
    37261 ataaggcggg actaacctga taaacaggca tcccctcctt atcaaagaaa taagtaaaca
    37321 tcatcaaatc taccgcttta atccgctctg ccggcaaaac ggcaaacctg tccaagaaaa
    37381 accgcatcgc ctcacgcgaa atgatatagc cagccgtccc acaatgttcg ctctccagca
    37441 aaggaaatga ccggttttca taattcagga ctttatccgg tctgacaata actttcgcaa
    37501 acatcgtttc caagcggacg acaaaggcgg aatccttatc aaaacgctct tccaaccaag
    37561 tatcttcggc aaggaacttt tctgcgtctt tgccaaacag gacatcatcc tcaaatacgg
    37621 caacatacgg tacgccttcg tccaatgcct gtttccacaa tacggcgtgg ctcataaagc
    37681 aggctttttc cactccgctc aaataggggt gcgccgacaa gccggggacg agttccgcca
    37741 tcgcccgttc cagcctttca gacggcatca gtgcgtcgaa aaactggaac gggatgccgc
    37801 gactgccgaa ggtatcggca atgtgcgccc tgcgctctgc ggcggaagcc aagctgataa
    37861 cgtggttttg catgatttat cctgttcggt ttcaatagct gcggtatttc caagggcgta
    37921 acattttttt caggattttc ctatattgcg gtgcggcaaa cagcttcctc atcacgcggt
    37981 ctgtcaagaa atccagcaaa tcggtcaaag aatacttttc caacgacaag aaatattcgt
    38041 acaggaacag gcggaggcat ccgatatcct gtccggacaa ggctttttcg tacaacgcat
    38101 atgccgttga tttcaaaagc ccgtaattca ggcagtccgc cccgacggct atgcctgccg
    38161 ccttccaata ccccgccctg atttcttctt tgattttcca cgccgtcctg cgctgttgca
    38221 ggttgtattt ggaagaagtc tggtcttgat ggaagcggta tttgaccaag gcttcgggat
    38281 aataagccag cctgcccagt ttgccggctt cgtaccaaaa cttatagtct tcggcgtgga
    38341 tataggctgg atcgaaccgc aaaccgccgt caatgacgct gcgcctcata atcatcgtgt
    38401 tgttgtgtat ggggttgccg aaagggaaaa cggcgacaat gtcttcatgc cgggtcggtt
    38461 tgtcccaaat tgcgccgttt cgggcaatgg cggcaagcac gcttttattg ttttcttccg
    38521 acaaaacttc ca
//
LOCUS       DAWTQS010000013        36669 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.013, whole genome shotgun sequence.
ACCESSION   DAWTQS010000013 DAWTQS010000000
VERSION     DAWTQS010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 36669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36669
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.013"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            68..1606
                     /gene="murJ"
                     /locus_tag="WNA25_000663"
     CDS             68..1606
                     /gene="murJ"
                     /locus_tag="WNA25_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFA9823477.1"
                     /translation="MNMLGALAKVGSLTMVSRVLGFVRDTVIARAFGAGMATDAFFVA
                     FKLPNLLRRVFAEGAFAQAFVPILAEYKETRSKEATEAFIRHVAGMLSFVLIVVTALG
                     ILAAPWVIYVSAPGFTKDADKFQLSISLLRITFPYILLISLSSFVGSILNSYHKFGIP
                     AFTPTFLNISFIVFALFFVPYFDPPVTALAWAVFVGGILQLGFQLPWLAKLGFLKLPK
                     LNFKDAAVNRVMKQMAPAILGVSVAQISLVINTIFASYLQSGSVSWMYYADRMMELPG
                     GVLGAALGTILLPTLSKHSANQDTEQFSALLDWGLRLCMLLTLPAAAGLAVLSFPLVA
                     TLFMYREFTLFDAQMTQHALIAYSFGLIGLIMIKVLASGFYARQNIKTPVKIAIFTLI
                     CTQLMNLAFIGPLKHAGLSLAIGLGACINAGLLFFLLRKHGIYRPGRGWAAFLAKMLL
                     ALAVMCGGLWAAQACLPFEWAHAGGMRKAGQLCILIAVGGGLYFASLAALGFRPRHFK
                     RVES"
     gene            1739..2695
                     /locus_tag="WNA25_000664"
     CDS             1739..2695
                     /locus_tag="WNA25_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFA9823478.1"
                     /translation="MILTPPDTPFFLRNGNADTIAAKFLQHPAPAYRREMLPDSTGKT
                     KTAYDFSGGISPDAPLVVLFHGLEGSSRSHYAAELMLAVRNRGWHGAVVHFRSCGGVA
                     NTAPVFYHLGDTAEIAFALDTLTARYREIYAVGVSLGGNAPAKYLGEQGKKALPHASA
                     AVSAPVDAEAAGSRFDSGITRLLYTRYFLRTLIPKARSLQGFQTAFAAGCKTLGEFDD
                     RFTAPLHGFADRHDYYRQTSCKPLLKHVAKPLLLLNAANDPFLPPEALPRADEASEAV
                     TLFQPAHGGHAGFVSSTGGRLHLQWLPQTVLSYFDSFRTNRH"
     gene            2746..3528
                     /gene="trpC"
                     /locus_tag="WNA25_000665"
     CDS             2746..3528
                     /gene="trpC"
                     /locus_tag="WNA25_000665"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HFA9823479.1"
                     /translation="MTDILNKILATKAQEVAAQKAAVNAEHIRALAAEAAPVRSFIDS
                     IRGKHRLNLPAVIAEIKKASPSKGLIRPDFRPAEIARAYENAGAACLSVLTDEPYFQG
                     SPEYLKQAREAVLLPVLRKDFIIDEYQVYQARAWGADAVLLIAAALEQGQLERFEALA
                     HELGMTVLLELHDETELEKCRNLTTPLRGVNNRNLRTFEVSLDQTLSLLPALEGKTVV
                     TESGITGKADVEFMRARGVHTFLIGETFMRADDIGAEVGKLF"
     gene            3591..5894
                     /gene="recQ"
                     /locus_tag="WNA25_000666"
     CDS             3591..5894
                     /gene="recQ"
                     /locus_tag="WNA25_000666"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFA9823480.1"
                     /translation="MTHRPTARQILHEVFGYPEFRGRQEDVINTLAGGGSLTVLMPTG
                     GGKSLCYQIPALMREGVAVVVSPLIALMNDQVASLHVAGIEAAAVNSGTSADEAREIA
                     DKLAQGRLKLLYVAPERLVTDRFLRFLDQQTVSLFAIDEAHCVSRWGHDFRPEYQQLG
                     MLAERYPNIPRIALTATADAATRADIKHYLHLDDASEFVSSFDRTNIYYQVIEKNNGK
                     KQLLDFIRKEMTGQSGIVYCLSRKKVEDAAQFLRENGLNAIPYHAGLSMDVREENQRR
                     FTHEDNIIVVATVAFGMGIDKPDVRFVAHLDMPQSVEHFYQESGRAGRDGLPAVSWLC
                     YGLNDWVLLRERIAEGNSDEVQKQIEMQKLDAMLSVCETAACRRVLLLKHFGEASEPC
                     GHCDNCLHPPVRFDGTVLVQKLLSCVYRAGQRFAAGYITNLLRGKSDDWIRGNRHEQL
                     STFGIGAELSDKEWRSVIRQCISLGYLTVNIARYQALQLTEAAKKILKGETEVMLRPL
                     KRDKPATRTLKDNWLRTEREERLWQALRVWRMKQAEAEGIPAYMIFGDKTLRDLVEKM
                     PQNLNGLHDIYGLGEAKTERFGHGILKVCQNAADFSHDAVIRPQTEREQQLRQKLEAW
                     RYEQARAENCALHTVLSDESLADMLAATPETETDLEAVHGLGSVRAAKYGRDILAVCR
                     PFSDGIDETAKHKRCLMRALIQWCNETAKHEQSEPYRILSKAALRAIAAKQPEGLAEL
                     AAVYGVGEEKAARYGAAVLAVLERDAV"
     gene            complement(5931..7232)
                     /locus_tag="WNA25_000667"
     CDS             complement(5931..7232)
                     /locus_tag="WNA25_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein"
                     /protein_id="HFA9823481.1"
                     /translation="MYWERGLHMYKASAVVPTGYVRVGNTAPLCGEDTQRYASFWGDG
                     YDVYRQLRWRQIPEKQRKAFKKAAKSKNTVMFAGREYGISKQNLSDVWDDFEDAMELK
                     AFPCLSSLFLTKWHKNLYEYLEEYPFITRLCLENHGQTVLDFSNTRITDLSVDMTGVE
                     SLYLNEGLDSLNLKGEIKENCKVCTAGKGAGLILEVGKSVPKVRGLENLTAVNVMGIA
                     DFDMQNLSETYPKLKTIRLWGKPGNIANFSAVSGFEDLEVFTAVDLFGFGADDIPHPD
                     RLPKLHRLWMSSLPEEAAKAVKKLYKKRKEDGLDLWIEKARKPEWLAQNFDNPFRDWD
                     GAEHIPKSHAKKAAELYRKTRAGVVKLLGNPPENTGEGLAEAVKAYTGGFNKMDKKHF
                     IDTVEREDIAEALETILDLIPDGSCADKEKLFEIFDKNRNF"
     gene            7426..7533
                     /locus_tag="WNA25_000668"
                     /pseudo
     CDS             7426..7533
                     /locus_tag="WNA25_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(7614..8402)
                     /locus_tag="WNA25_000669"
     CDS             complement(7614..8402)
                     /locus_tag="WNA25_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFA9823482.1"
                     /translation="MNPQDKCWQVHRHLAEHTDQRLTLVRNAPGHILLAGADADISRS
                     LLAKRYPPAVFEEYDSRADFLAAAAAARKGGFWQKLTGRGVVQHCQSPTVPLPEACAD
                     MLWSNLGLLAAEQILPVLHNWARALKTDGLLFFTCFGRDTLAELKSRLKENGIESRSV
                     MFPDMHDLGDMLAENGFYDPVTDTAKLVLDYKKAETFWADMDTLGVWRAVAWDDENAA
                     RSCVGAIFEREGGLGITLETVYGHAVKKLVLPQGENVVRFFPKR"
     gene            complement(8399..9280)
                     /gene="bioH"
                     /locus_tag="WNA25_000670"
     CDS             complement(8399..9280)
                     /gene="bioH"
                     /locus_tag="WNA25_000670"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFA9823483.1"
                     /translation="MRKLAKSVLQPAQMPSETPDEPWHNTQRLIVGASAMRRQQERKF
                     MPDAAKKVYLIHGWGANRHAFDDLMPRLPATWPVSAVDLPGHGDAPFAQPFDIEAAAD
                     GIAAQIDTSADILGWSLGGLVALYLAARHPDKVRSLCLTASFARLTAAEDYPEGLAAP
                     ALGKMVGAFRTDYAKHIKQFLQLQLLHTPDAAEIIGRILPDLARCGTPQALQEALDAA
                     ERADVRHLLDKIDVPVLLVFGGKDAITPPRMGEYLHRHLKGSRLVVMEKAAHAPFLSH
                     AEVFAALYRDFVEGGLT"
     gene            9264..9872
                     /locus_tag="WNA25_000671"
     CDS             9264..9872
                     /locus_tag="WNA25_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFA9823484.1"
                     /translation="MASFRTDAANSCPLCFRHVQGGAVCGGCQKKPPAFDRMWASLHY
                     EPPVSNMIRALKHLADLGMAQPLADLTMQNPPDRLSDECFDFVLPVPLSRERLLQRGF
                     NQSESIVGLLAQRYGWQILPRHTVFRHHRPPQSTLKGGERRRNIKNAFEIRTPIPENC
                     NILLIDDVFTTGATLDELAKTLKKSGANRICCWTLARTPMKK"
     gene            9937..10401
                     /gene="trmL"
                     /locus_tag="WNA25_000672"
     CDS             9937..10401
                     /gene="trmL"
                     /locus_tag="WNA25_000672"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFA9823485.1"
                     /translation="MLTIVLYQPEIPPNTGNIIRLCANTGADLHLVKPLGFPLDSAKM
                     KRAGLDYHEFASLTVHENFDDCLKSLAGRRIFALTTKGTARPDETAFQKGDVLLFGPE
                     TRGLPADILDSLPAAQKIRLPMRPGSRSMNLSNTVSVILFEAWRQHGYAGGV"
     gene            10529..11215
                     /locus_tag="WNA25_000673"
     CDS             10529..11215
                     /locus_tag="WNA25_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HFA9823486.1"
                     /translation="MTLTRKTLFLLTAAFGTHSLQTASADAVVKAEKLHASANRSYKV
                     AEFTQTGNASWYGGRFHGRKTSGGDRYDMNAFTAAHKTLPIPSHVRVTNTKNGKSVIV
                     RVNDRGPFHGNRIIDVSKAAAQKLGFVSQGTAHVKIEQIVPGQSAPVAENKDIFIDLK
                     SFGTEHEAQAYLNQAAQNFAASSSSPNLSVEKRRYEYVVKMGPFASQERAAEAEAQAR
                     GMVRAVLTSG"
     gene            11276..11602
                     /locus_tag="WNA25_000674"
     CDS             11276..11602
                     /locus_tag="WNA25_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFA9823487.1"
                     /translation="MNIKHLLLTAAATALLGISAPALAHHDGHGDDDHGHAAHQHGKQ
                     DKIISRAQAEKAAWARVGGKITDIDLEHDDGRPHYDVEIVKNGQEYKVVVDARTGRVI
                     SSRRDD"
     gene            11684..12268
                     /gene="ruvA"
                     /locus_tag="WNA25_000675"
     CDS             11684..12268
                     /gene="ruvA"
                     /locus_tag="WNA25_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFA9823488.1"
                     /translation="MISRLTGKLVEKNPPQIVIDVNGVGYEADVSMQTFYNLPPVGES
                     VQLFTQLIIREDAHLLFGFATAEERKTFRQLIKVGGIGAKTALGILSAMTADELARAV
                     AEEDVKRLSSAPGIGKKTAERMVLELRGKLVAHTVTDGLFAASPAADETEDIVSTLLA
                     LGYNEREAKAAVKGVPKGTDVGEGVRLALKNLLK"
     gene            12268..12405
                     /locus_tag="WNA25_000676"
     CDS             12268..12405
                     /locus_tag="WNA25_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823489.1"
                     /translation="MPSEGGAAFALTKPCVSAPRCLPDGFSRKMALLSLLSDGIVFDA
                     V"
     gene            12431..14287
                     /locus_tag="WNA25_000677"
     CDS             12431..14287
                     /locus_tag="WNA25_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFA9823490.1"
                     /translation="MLNKIFSWFESRIDPYPEAAPKTPEKGLCRFVWSSMDGVRKWIA
                     ALAALTAGIGIMEALIFQFMGKIVEWLGKYAPAELFAEKGWELAAMAAMMAFSVVWAF
                     AASNVRLQTLQGVFPMRLRWNFHRLMLNQSLGFYQDEFAGRVSAKVMQTALALRDAVM
                     TVADMVVYVSVYFITSGVILASLDSWLLLPFIGWIIGFASVMRLLIPRLGQTAARQAN
                     ARSLMTGRITDAYSNIATVKLFFHGAREAVYAKQSMEEFMVTVRAQMRLATLLHSCSF
                     IVNTSLTLSAAALGIWLWHNGQVGVGAVATATAMALRVNGLSQYIMWESARLFENIGI
                     VNDGMATLSKPHTILDKPQALPLNVPQGAIKFEHVDFCYEAGKPLLNGFNLNIKPGEK
                     VGLIGRSGAGKSTIVNLLLRFYEPQSGTVSIDGQDISGVTQESLRAQIGLVTQDTSLL
                     HRSVRDNIIYGRPDATDAEMVSAAERAEAAGFIPDLSDAKGRSGYDAHVGERGVKLSG
                     GQRQRIAIARVMLKDAPILLLDEATSALDSEVEAAIQESLDKMMEGKTVIAIAHRLST
                     IAAMDRLVVLDKGRIIEEGTHAELLEKRGLYAKLWAHQSGGFLSEHVEWQHD"
     gene            14292..15215
                     /gene="rsgA"
                     /locus_tag="WNA25_000678"
     CDS             14292..15215
                     /gene="rsgA"
                     /locus_tag="WNA25_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFA9823491.1"
                     /translation="MPSEHPFSDGISTPNPKETMNDTAQITAGYGRRYIVRTPDGTTY
                     EASTRKKRVDFACGDRVRISPVNAEQVVIEDFLPRQSLLYRQDAWKTKLIAANVTQLL
                     IVTAAVPSPSVRLLQRALLAAEAAGIRAVIVLNKADLPETALWLEKLKFYETLGYPVI
                     ETRVLENADSLRPVLQGHSNILLGQSGMGKSTLTNALLGSQTARTGDISAALDSGKHT
                     TTHARLYDLNGETQLIDSPGLQEFGLHHLQAADLPHYFPDFRHLVGQCRFHNCTHRAE
                     PGCAFKAAAETGAASPERLAFLQGITDELLG"
     gene            15241..15348
                     /locus_tag="WNA25_000679"
                     /pseudo
     CDS             15241..15348
                     /locus_tag="WNA25_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346047.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
     gene            15328..15552
                     /locus_tag="WNA25_000680"
     CDS             15328..15552
                     /locus_tag="WNA25_000680"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFA9823492.1"
                     /translation="MKKNTPKSFEEALSRLESLTQSMQGEMPLEDALAAYQEGNELVR
                     YCQTKLAQVEQKLQVLDADGTKELNLESDE"
     gene            15536..16432
                     /locus_tag="WNA25_000681"
     CDS             15536..16432
                     /locus_tag="WNA25_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFA9823493.1"
                     /translation="MNPTNDLKAWQQRAQAQTELLLERFLPSGNEIPHTLHEAMRYAA
                     LDGGKRLRPMLVLAASELGGAVADAVGQAMAAIEMIHVYSLVHDDMPAMDNDSLRRGK
                     PTCHIKYGEATALLTGDALQTQAFDVLSRPTELPAARQLAMLSVLAKAGGSAGMAGGQ
                     AIDLANVGKQMVQADLERMHSLKTGALIRAAVLLGATACPDLSDAELAVLDAYAAKLG
                     LAFQVIDDVLDCEADTATLGKTAGKDADNDKPTYVKLMGLEAARSYAHKLVAEAVALL
                     EPFGDKALRLRQLAEFAVARKY"
     gene            complement(16549..16848)
                     /locus_tag="WNA25_000682"
     CDS             complement(16549..16848)
                     /locus_tag="WNA25_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HFA9823494.1"
                     /translation="MTASMYILLVLALIFANAPFLTTRLFGVAALKRKHFGHHLIELA
                     AGFALTASLAYILESRAGAVHNQGWEFYATVVCLYLIFAFPCFVRRYFWHTRNRE"
     gene            complement(16960..18405)
                     /gene="nuoN"
                     /locus_tag="WNA25_000683"
     CDS             complement(16960..18405)
                     /gene="nuoN"
                     /locus_tag="WNA25_000683"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFA9823495.1"
                     /translation="MNWSDLNLMPALPEVALLSLLVLLLPADLWASDDKCRWTHYGAL
                     ATVAVTAAVQLAVWEQGSTSSFNGMYIADGMSRLAKMVLYALTFVLFVYAKPYNQVRG
                     IFKGEFYTLSLFALLGMSVMVSAGHFLTAYIGLELLSLALYALIALRRDSGFAAEAAL
                     KYFVLGALASGLLLYGISMVYGATGSLEFAGVLASSFNEEANEWLLKLGLVFIVVAVA
                     FKLGAVPFHMWMPDVYHGAPTSVTALVGTAPKIAAVVFAFRILVTGLGTVHHDWSLMF
                     ALLAAASLLVGNLAAIMQTNIKRMLAYSTVSHTGFILLAFMAGAVGFAAGLYYAITYA
                     LMAAAGFGVLMVLSDGDNECENISDLAGLNQHRVWLAFLMLLVMFSMAGIPPLMGFYA
                     KFGVIMALLKQGYVWLSVFAVVMSLVGAFYYLRVVKVMYFDESGRARPAAGGNNAAKS
                     LLSVNALLLVLWGIMPQTVIDWCAKALENTL"
     gene            complement(18415..19911)
                     /locus_tag="WNA25_000684"
     CDS             complement(18415..19911)
                     /locus_tag="WNA25_000684"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFA9823496.1"
                     /translation="MFSNYLLSLAIWIPIAAGVLVLATGKDSRAPLARVLAFMGALAG
                     FLVTLPLFTGFDRLSGGYQFTEFHEWIPLLKINYALGVDGISVLFIILNAFITLLVVL
                     AGWEVIQKRPAQYMAAFLMMSGLINGAFAAQDAILFYVFFEGMLIPLYLIIGVWGGPR
                     RVYASVKLFLYTLTGSLLMLVAMVYLYYQTGSFSIVDFQNIKQIPLGVQQLLFVAFFL
                     SFAVKVPMFPVHTWLPDAHVEAPTGGSMVLAAITLKLGAYGFLRFILPIMPDAARYFA
                     PVIIVLSLIAVIYIGMVALVQTDMKKLVAYSSISHMGFVTLGMFLFVDGQLDDWALKG
                     AVIQMISHGFVSAAMFMCIGVMYDRLHTRNIADYGGVVNVMPKFAAFMMLFGMANAGL
                     PATSGFVGEFMVVMGAVKVNFWVGALAAMTLIYGASYTLWMYKRVIFGAIHNPHVAEM
                     KDINCREFAILAVLAVAVLGMGLYPNAFIEVVHQAANDLIAHVAQSKI"
     gene            complement(20025..21188)
                     /locus_tag="WNA25_000685"
     CDS             complement(20025..21188)
                     /locus_tag="WNA25_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HFA9823497.1"
                     /translation="MTQLFLHSDTPNEKELISIYEKAVSEAVELYIFSAYLTHWDVKN
                     KLNSKLQEFKFIFGQDFGLSKKEAIRKVLKWLPSHLKFTLMVAQGIQGFHPKAVFWKN
                     DKNEYYALIGSSNLTHAAFNSNYEANILTKISEQDFIKVKSWADEIAMKSIPVSEDWL
                     EEYQEAEINYKKSTVRQSVMDKLFMEMPNYNQELIAARRKQMRNHQTVCNQLKNLIKQ
                     CAAGKIDNNDFYGEFNKLWSWKSENKGEGVGNRFQDKTWKRTGKSSDFRKLCIAIQSV
                     FDAPLTERDNVVAKQIDWLKECGVSTRGSVFSEMLCQEYPDRYPVLNAPIKKFLEENK
                     FKSAKGASEGSKYIDLSMKLRALLAMQSEIKDLAELDVLVQAEYRNRTDIDWE"
     gene            complement(21372..23396)
                     /gene="nuoL"
                     /locus_tag="WNA25_000686"
     CDS             complement(21372..23396)
                     /gene="nuoL"
                     /locus_tag="WNA25_000686"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFA9823498.1"
                     /translation="MNDMTLYLIIALVPLAGSLIAGLFGNKIGRAGAHTVTILGVAVS
                     AVLSAYVLWGFLNGSRAKFDENVYTWLTMGGLDFSVGFLVDTMTAMMMVVVTGVSLMV
                     HIYTIGYMHDEKVGYQRFFSYISLFTFSMLMLIMSNNFIQLFFGWEAVGLVSYLLIGF
                     YFKRPGATFANLKAFLINRVGDFGFLLGIGLVLAYFGGSLRYQDVFAYLPNVQNATIQ
                     LFPGVEWSLITVTCLLLFVGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGL
                     FMVSRMSPIYEMSSTALSVIMVIGAITALFMGFLGVIQNDIKRVVAYSTLSQLGYMTV
                     ALGASAYSVAMFHVMTHAFFKALLFLAAGSAIIGMHHDQDMRHMGNLKKYMPITWLTM
                     LIGNLSLIGTPFFSGFYSKDSIIEAVKYSTLPGSGFAYFAVLASVFVTAFYAFRQYFM
                     VFHGEEKWRSLPEHHSDGHGEEHHGLGKNDNPHESPLVVTLPLILLAIPSVIIGYIAI
                     EPMLYGDFFKDVIFVNADAHPTMHIMKEEFHGALAMVSHSLHSPVLYLATAGVLSAWL
                     LYVKLPHLPAKIAQAFRPVYVLFENKYYLDALYFNVFAKGTRALGNFFWKVGDTAIID
                     NGIVNGSAKLVGAIAAQVRKVQTGFIYTYAAAMVFGVLVLLGMTFWGLFR"
     gene            complement(23399..23704)
                     /gene="nuoK"
                     /locus_tag="WNA25_000687"
     CDS             complement(23399..23704)
                     /gene="nuoK"
                     /locus_tag="WNA25_000687"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFA9823499.1"
                     /translation="MITLTHYLVLGALLFGISAMGIFMNRKNVLVLLMSIELMLLAVN
                     FNFIAFSQHLGDTAGQIFVFFVLTVAAAESAIGLAIMVLVYRNRQTINVADLDELKG"
     gene            complement(23701..24372)
                     /locus_tag="WNA25_000688"
     CDS             complement(23701..24372)
                     /locus_tag="WNA25_000688"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFA9823500.1"
                     /translation="MTFSVILFYILAAIVLYGAVRTVTAKNPVHAALHLVLTFCVSAM
                     IWMLMQAEFLGVTLVVVYVGAVMVLFLFVVMMLNIDIEEMRAGFWRHAPVAGVVGTLL
                     AVALILILVNPKTDLAAFGLMKDIPADYNNIRDLGSRIYTDYLLPFELAAVLLLLGMV
                     AAIALVHRKTTNPKRMDPADQVKVRADQGRMRLVKMEAVKPQVESAEESEVSDGLKTE
                     GEGKA"
     gene            complement(24384..24863)
                     /gene="nuoI"
                     /locus_tag="WNA25_000689"
     CDS             complement(24384..24863)
                     /gene="nuoI"
                     /locus_tag="WNA25_000689"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFA9823501.1"
                     /translation="MANLVKTFLLGELVKGMGVTLKNFFARKDTIYFPEEKTPQSVRF
                     RGLHAQRRYPNGEERCIACKLCEAVCPAMAINIESEEREDGTRRTKRYDIDLTKCIFC
                     GFCEEACPTDAIVETHIFEYHGEKKGDLHMTKPILLAIGDKYEAEIAKRKAADAPYR"
     gene            complement(24944..26020)
                     /gene="nuoH"
                     /locus_tag="WNA25_000690"
     CDS             complement(24944..26020)
                     /gene="nuoH"
                     /locus_tag="WNA25_000690"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFA9823502.1"
                     /translation="MQEWFQNLFAATLGLGDLGITVGLVVSVIVKIVIILIPLILTVA
                     YLTYFERKVIGFMQLRVGPNVTGPQGLIQPFADVFKLLFKEVTRPKLSNKALFYIGPI
                     MSLAPSFAAWAVIPFNEEWVLTNINIGLLYILMITSLSVYGVIIAGWASNSKYSFLGA
                     MRASAQSISYEIAMSAALVCVVMVSGSMNFSDIVAAQAKGIAGGSVFSWNWLPLFPIF
                     IVYLISAVAETNRAPFDVAEGESEIVAGHHVEYSGFAFALFFLAEYIFMILIAALTSL
                     MFLGGWLSPFPQSWGIVGTPSAFWMFVKMAAVLYWYLWIRATFPRYRYDQIMRLGWKV
                     LIPIGFAYIVVLGVWMISPLNLWK"
     gene            complement(26023..28284)
                     /locus_tag="WNA25_000691"
     CDS             complement(26023..28284)
                     /locus_tag="WNA25_000691"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HFA9823503.1"
                     /translation="MLQIEIDGKQVSVEQGATVIEAAHKLGTYIPHFCYHKKLSIAAN
                     CRMCLVDVEKAPKPLPACATPVTDGMIVRTHSAKAREAQEGVMEFLLINHPLDCPTCD
                     QGGECQLQDLAVGYGKTTSRYTEEKRSVVGKDMGPLVSAEEMSRCIHCTRCVRFTEEI
                     AGLQEIAMVNRGEHSEIMPFIGKAVETELSGNVIDLCPVGALTSKPFRFNARTWELNR
                     RKSVSAHDALGSNLIVQTKDHTVRRVLPLENEAINECWLSDRDRFAYEGLYHESRLKN
                     PKIKQGGEWMDVDWKTALEYVRSAIECIAKDGNQNQVGVWANPMNTVEELYLAKKFAD
                     GLGVKNFATRLRQQDKRLSDGLKGAQWLGQSIESLADNDAVLVVGANLRKEQPLLTAR
                     LRRAAKDRMALSVLAGSKEELFMPLLSQEAAHPDEWAGRLKNLSADAEHAVTASLKNA
                     EKAAVILGAEVQNHPDYAAIYAAAQELADATGAVLGILPQAANSVGADVLGVNSGESV
                     AEMANTPKQAVLLLNVEPEIDTVDGAKAVAALKQAKSVMAFTPFVSETLLDVCDVLLP
                     IAPFTETSGSFINMEGRLQSFHGVVQGFGDSRPLWKVLRVLGNLFDLKGFEYHDTAAI
                     LKDALDAESLPSKLDNRSTWAGEGVQTASDRLVRVGGVGIYHTDSIVRRSAPLQETSH
                     AAVPAARVNPNTLARLGLQDGQTAVAKQNGASVSVAVKADAGLPENVVHLPLHTENAA
                     LGALMGTIELAGA"
     gene            complement(28574..28997)
                     /locus_tag="WNA25_000692"
                     /pseudo
     CDS             complement(28574..28997)
                     /locus_tag="WNA25_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(28997..30298)
                     /gene="nuoF"
                     /locus_tag="WNA25_000693"
     CDS             complement(28997..30298)
                     /gene="nuoF"
                     /locus_tag="WNA25_000693"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFA9823504.1"
                     /translation="MAIYQSGVIFDQVDTANPDCWTLDEYVKRGGYTALRKILSENIS
                     QTDVIDEVKTSGLRGRGGAGFPTGLKWSFMPRSFPGEKYVVCNTDEGEPGTFKDRDII
                     MFNPHALIEGMIIAGYAMGAKAGYNYIHGEIFEGYQRFEAALEQARAAGFLGKNILGS
                     DFEFELFAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTET
                     FSSVPFIIRDGGQAFADKGIPNAGGTKLFCISGHVERPGNYEVPLGTPFAEVLKMAGG
                     MRGGKKLKAVIPGGSSAPVLPADIMMQTNMDYDSISKAGSMLGSGAIIVMDEDVCMVK
                     ALERLSYFYYDESCGQCTPCREGTGWLYRIVRRIVEGKGRMEDLDLLDSVGNQMAGRT
                     ICALADAAVFPVRSFTKHFRDEFAHYIEHGGPMKEHKWGGW"
     gene            complement(30688..31161)
                     /gene="nuoE"
                     /locus_tag="WNA25_000694"
     CDS             complement(30688..31161)
                     /gene="nuoE"
                     /locus_tag="WNA25_000694"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFA9823505.1"
                     /translation="MLSAKSLKQIDIELAKYPADQRRSAIMGALRIAQTEKGWLAPET
                     IAFVADYIGITPAQAYEVATFYNMYDLEPVGKYKLTVCTNLPCALRGGMATGEYLKQK
                     LGIGYGETTPDGKFTLVEGECMGACGDAPVMLVNNHSMCSFMTEEAIEKKLAELE"
     gene            complement(31161..32417)
                     /gene="nuoD"
                     /locus_tag="WNA25_000695"
     CDS             complement(31161..32417)
                     /gene="nuoD"
                     /locus_tag="WNA25_000695"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HFA9823506.1"
                     /translation="MANKLRNYTINFGPQHPAAHGVLRMILELDGEQIVRADPHIGLL
                     HRGTEKLAETKTYLQALPYMDRLDYVSMMVNEQAYCLAVEKLAGIDVPIRAQYIRVMF
                     AEVTRILNHLMGIGSHAFDIGAMTAILYAFRDREELMDLYEAVSGARMHAAYFRPGGV
                     YRDLPGFMPKYESSKFRNAKVLKQLNESREGTMLDFIDAFCERFPKNIDTLETLLTDN
                     RIWKQRTVGIGVVSPERAMQKGFTGVMLRGSGVEWDVRKTQPYEVYDKMDFDIPVGVN
                     GDCYDRYLCRMEEMRQSVRIIKQCADWLRVNPGPVITANHKFAPPKRTEMKTGMEDLI
                     HHFKLFTEGMHVPEGETYTAVEHPKGEFGVYIISDGANKPYRLKIRAPGFAHLQGMDE
                     MAKGHMLADVVAIIGTQDIVFGEVDR"
     gene            complement(32407..33000)
                     /locus_tag="WNA25_000696"
     CDS             complement(32407..33000)
                     /locus_tag="WNA25_000696"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HFA9823507.1"
                     /translation="MASIQNLYETVVGVLGDQAGKVISALGEITVECLPEHYISVMTA
                     LHDHEDLHFELLVDLCGVDYSTYKNEAWQGKRFAVVSQLLSVKNNQRIRVRVWVSDDD
                     FPVVESVADIYNSADWYEREAFDLYGIMFNNHPDLRRILTDYGFVGHPFRKDFPISGY
                     VEMRYDEEQKRVIYQPVTIEPREITPRIVREENYGGQ"
     gene            complement(33013..33495)
                     /locus_tag="WNA25_000697"
     CDS             complement(33013..33495)
                     /locus_tag="WNA25_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HFA9823508.1"
                     /translation="MGIEGVLKKGFITTSADTVLNYMRTGSLWPVTFGLACCAVEMMH
                     AGMARYDLDRFGIIFRPSPRQADLMIVAGTLTNKMAPALRRVYDQLAEPRWVLSMGSC
                     ANGGGYYHYSYSVVRGADRVVPVDVYVPGCPPTAEALIYGLIQLQQKIKRTSTIARDE
                     "
     gene            complement(33486..33842)
                     /locus_tag="WNA25_000698"
     CDS             complement(33486..33842)
                     /locus_tag="WNA25_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HFA9823509.1"
                     /translation="MLSAYFPVFVFILVGLAAGVLFILLGTILGPKRHYAEKDAPYEC
                     GFEAFENARMKFDVRYYLVAILFILFDLEVAFMLPWAVVFKDLGAYGFWSMLVFIVVL
                     TVGFVYEWKKGALEWE"
     gene            complement(34335..35786)
                     /locus_tag="WNA25_000699"
     CDS             complement(34335..35786)
                     /locus_tag="WNA25_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HFA9823510.1"
                     /translation="MLSFLSGLLSLGIEVLWVRMFSFAAQSVPQAFSFTLACFLTGIA
                     VGAYFGKRICRSRFVDIPFIGQCFLWAGIADFLILGAAWLLTGFSGFVHHAGIFITLS
                     AVVRGLIFPLVHHVGTDGNKSGRQVSNVYFANVAGSALGPVLIGFVILDFLSTQQIYL
                     LICLISAAVPLFCTPFQKSLRLNAVSVAVSLMFGILMFLLPDSVFQNIAGRPDRLIEN
                     KHGIVAVYHRDGDKVVYGANVYDGAYNTDIFNSVNGIERAYLPPSLKSGIRRIFVVGL
                     STGSWARVLSAIPEMQSMIVAEINPAYRSLIADEPQIAPLLQDKRVEIVLDDGRKWLR
                     RHPDEKFDLILMNSTWYWRAYSTNLLSAEFLKQVQSHLTPDGIVMFNTTHSPHAFATA
                     VHSIPYAYRYGHMVVGSATPVVFPNKELLKQRLSRLIWPESGRHVFDSSTVDAAAQKV
                     VSRMLIRMTEPSAGAEVITDDNMIVEYKYGRGI"
     gene            complement(35809..36429)
                     /locus_tag="WNA25_000700"
     CDS             complement(35809..36429)
                     /locus_tag="WNA25_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HFA9823511.1"
                     /translation="MNSTASKTLKGLSLVFFASGFCALIYQVSWQRLLFSHIGIDLSS
                     ITVIISVFMVGLGVGAYFGGRIADRFPSSIIPLFCIAEVSIGLFGLVSKGLISGLGHL
                     LVEADLPIIAAANFLLLLLPTFMMGATLPLLTCFFNRKIHNVGESIGTLYFFNTLGAA
                     LGSLAAAEFFYVFFTLSQTIALTACLNLLIAASVWLRYRKDGYGEH"
BASE COUNT         8944 a        10348 c         9387 g         7990 t
ORIGIN      
        1 gttaatccac tataaagtct gatcctgccg ccctaaaggg cggggtttca gccgaaaagg
       61 aaacacgatg aatatgcttg gagctttggc aaaagtcggc agcctgacga tggtgtcgcg
      121 cgttttggga tttgtgcgcg atacggtcat tgcgcgggca ttcggcgcgg gtatggcgac
      181 ggatgcgttt tttgtcgcgt tcaaactgcc caacctgctt cgccgcgtgt ttgcggaggg
      241 ggcgtttgcc caagcgtttg tgccgatttt ggcggaatat aaggaaacgc gttctaaaga
      301 ggcgacggag gcttttatcc gccacgttgc gggaatgctg tcgtttgtgc tgatcgtcgt
      361 taccgcgctg ggcatacttg ccgcgccttg ggtgatttat gtttccgcgc ccggctttac
      421 caaagacgcg gacaagttcc aactttccat cagcctgctg cggattacgt ttccttatat
      481 attattgatt tctttgtctt cttttgtcgg atcgatactc aattcctacc ataagttcgg
      541 cattcccgcg tttacgccca cgtttttaaa catctctttt atcgtattcg cactgttttt
      601 cgtgccgtat ttcgatccgc ccgttaccgc gctggcgtgg gcggtttttg tcggcggtat
      661 tttgcagctc ggtttccaac tgccgtggct ggcgaaactg ggctttttga aactgcccaa
      721 actgaatttc aaagatgcgg cggtcaaccg cgtcatgaaa cagatggcgc ctgcgatttt
      781 gggcgtgagc gtggcgcaaa tttctttggt tatcaacacg attttcgcgt cttatctgca
      841 atcgggcagc gtttcatgga tgtattacgc cgaccgcatg atggagctgc ccggcggcgt
      901 gctgggggct gcactcggta caattttgct gccgactttg tccaaacact cggcaaacca
      961 agatacggaa cagttttccg ccctgctcga ctggggtttg cgcctgtgca tgctgctgac
     1021 gctgccggcg gcggccggac tggcggtatt gtcgttcccg ctggtggcga cgctgtttat
     1081 gtaccgagaa ttcacgctgt ttgacgcaca aatgacgcaa cacgcgctga ttgcctattc
     1141 tttcggttta atcggtttaa ttatgattaa agtgttggca tccggctttt atgcgcggca
     1201 aaacatcaaa acgcccgtca aaatcgccat cttcacgctc atctgcacgc agttgatgaa
     1261 cctcgccttt atcggtccgt tgaaacacgc cgggctttcg ctcgccatcg gcctgggcgc
     1321 gtgcatcaac gccggattgt tgttcttcct gttgcgcaaa cacggtattt accggcccgg
     1381 caggggttgg gcggcgttct tggcgaaaat gctgctcgcg ctcgccgtga tgtgcggcgg
     1441 actgtgggcg gcgcaggctt gcctgccgtt cgaatgggcg cacgccggcg gaatgcggaa
     1501 agcggggcag ctctgcatcc tgattgccgt cggcggcgga ctgtatttcg catctctggc
     1561 ggctttgggc ttccgtccgc gccatttcaa acgcgtggaa agctgaccga tgccgatatc
     1621 ctttttcgga aaacgctttt tccgcgccta tatccgggcg cgcatcgccc gcgtccgcac
     1681 cggccggacg ggctgccgca tcaccaatgc cgtctgaaaa cagaaaaacc gataccttat
     1741 gattttgaca ccgccggaca cgcccttttt cctccgcaac ggcaatgccg acacgattgc
     1801 cgccaaattc ctgcaacacc ccgcacccgc ataccgccgc gagatgcttc ccgacagcac
     1861 gggtaaaacc aaaaccgcct acgacttttc aggcggcatt tcgcccgatg cgccgctggt
     1921 cgtgctgttt cacggtttgg aaggaagcag ccgcagccat tacgcggccg aactgatgct
     1981 cgcggtacgc aatcggggtt ggcacggcgc agtcgtccat ttccgcagct gcggcggcgt
     2041 agcgaacacc gccccggtgt tctaccactt gggtgatacc gccgaaatcg cctttgcttt
     2101 ggacacgctc accgcgcgtt accgtgaaat atacgccgtc ggcgtatcgc tgggcggcaa
     2161 cgcgccggca aaatatttgg gcgaacaggg caaaaaggca ttgccgcacg cctcggccgc
     2221 cgtatccgcc cccgttgatg cagaggcggc aggcagccgc ttcgacagcg gcatcacgcg
     2281 gctgctctac acgcgctact tcctccgcac actgataccc aaagcacgtt cgctccaagg
     2341 ttttcagacg gcatttgccg cagggtgcaa aacactgggc gagtttgacg accgtttcac
     2401 cgcaccgctg cacggctttg ccgaccggca cgactactac cgccaaactt cctgcaaacc
     2461 gctgctcaaa cacgttgcca aaccgctgct cctgctcaat gccgccaacg accccttcct
     2521 gccgcccgaa gccctgcccc gtgcggacga agcgtccgaa gccgttaccc tgttccaacc
     2581 tgcacacggc gggcacgccg gctttgtcag cagcaccggc ggcaggctgc acctgcaatg
     2641 gctgccgcag accgtcctgt cctattttga cagcttccgc acaaacaggc attaacggtt
     2701 tgatgctaat attccccctt tccccggaca aatacggaac acgacatgac cgacatcctc
     2761 aataaaatcc ttgccaccaa ggcacaggaa gttgccgccc aaaaagccgc cgtcaacgcc
     2821 gaacacatcc gcgcacttgc cgcagaagcc gcgcccgtcc gcagcttcat cgattcgata
     2881 cgcggcaaac accgcctaaa cctgcccgcc gtcattgccg aaatcaaaaa ggcaagcccg
     2941 agcaaagggt taatccgccc cgacttccgc cctgccgaga ttgcccgcgc ctatgaaaac
     3001 gccggggcgg cgtgtttgtc ggtactgacc gacgaaccct atttccaagg ttcgcccgaa
     3061 tacctcaaac aggcgcgcga agccgtattg ctgcccgtgc tgcgcaaaga cttcatcatc
     3121 gacgaatacc aggtttatca ggcgcgcgca tggggggcgg atgccgtcct gctgattgcc
     3181 gccgcactgg aacagggaca attggaacgc tttgaggcgc tggcgcacga attgggcatg
     3241 accgtcctgc tcgagctgca cgacgaaacc gaattggaaa aatgccgcaa cctgaccacg
     3301 ccgttgcggg gcgtaaacaa ccgcaacctg cggacttttg aagtctccct cgaccaaacc
     3361 ctgtcgctgc tgcccgcgct ggaaggcaaa accgtcgtta ccgaaagcgg cattacaggc
     3421 aaggcggatg tggaatttat gcgggcgcgc ggcgtgcata ccttcctgat cggcgaaacg
     3481 tttatgcgtg ccgacgatat tggggcggaa gtgggcaaac tcttctaaaa tcccgatttc
     3541 agacggcata ttgccgccga ccgaccaaca cccttaccgg acacggaaca atgacgcacc
     3601 gacccaccgc cagacagatt ttgcacgaag tattcggcta tcccgaattt cgcggcaggc
     3661 aggaggatgt catcaatact ttggcaggcg gcggcagttt gacggtgctg atgccgacgg
     3721 gcgggggcaa gtctttgtgt taccagattc cggcgctgat gcgcgaaggc gtggcggttg
     3781 tcgtatcgcc gctgattgcg ctgatgaacg accaagtggc cagcctgcat gtggccggga
     3841 ttgaagcggc ggcagtcaac agcggcacat cggcagatga ggcgcgcgag attgccgaca
     3901 agcttgccca aggccgtctg aagctgcttt atgtcgcgcc ggaacgcttg gttaccgacc
     3961 gctttttacg ttttctcgac caacaaaccg tcagcctgtt cgccattgac gaagcgcact
     4021 gcgtcagccg gtggggacac gatttccgcc ccgaatatca acagctcggt atgcttgccg
     4081 aacgctatcc gaacatcccg cgcatcgccc tgaccgccac cgccgatgcc gccacgcgcg
     4141 ccgacatcaa gcattatctg cacttggacg acgcgtccga atttgtctcc agctttgacc
     4201 gcacgaatat ttattatcag gttatcgaaa aaaacaacgg caaaaaacaa ttgctggatt
     4261 tcatccgcaa agaaatgacg gggcaaagcg gcattgtgta ttgcctaagc cgcaaaaagg
     4321 ttgaagatgc ggcgcagttt ttgcgtgaaa acggattaaa cgcgattccg tatcatgccg
     4381 gtttgagcat ggacgtgcgc gaggaaaacc aacgccgctt tacgcatgaa gacaatatta
     4441 tcgtggtggc gaccgtggcg ttcggcatgg gcatagacaa acccgacgtg cgctttgtcg
     4501 cccatctcga tatgccccag agtgtcgaac atttctatca ggaatcaggg cgcgccggcc
     4561 gggacgggct gcctgccgtg agttggctgt gttacggctt gaacgattgg gtgttgctgc
     4621 gcgaacggat tgccgaaggc aacagcgacg aggtgcaaaa gcaaatcgaa atgcaaaaac
     4681 tcgatgccat gctttccgtc tgcgaaaccg ccgcctgccg ccgcgtactg ctgctcaaac
     4741 atttcggcga agcatccgaa ccctgcggac attgtgacaa ctgcctgcat ccgcccgtac
     4801 ggtttgacgg cacggtgttg gtgcaaaaat tactcagctg cgtgtaccgc gccggacaac
     4861 gctttgccgc cggttacatc accaaccttt tgcgcggcaa aagcgacgat tggatacgcg
     4921 gcaaccggca cgagcaactg tccacattcg gcatcggcgc ggaattgtcc gacaaagaat
     4981 ggcgcagcgt catccgccag tgcatcagcc tcggctacct caccgtcaac attgcccgat
     5041 atcaggcatt gcaactgacc gaagccgcca aaaaaatcct caaaggcgaa accgaagtga
     5101 tgctgcgccc gctcaagcgc gacaagcccg ccacccgcac cctcaaagac aactggctgc
     5161 gtaccgaacg cgaagaacgc ctgtggcagg cattgcgcgt ttggcgcatg aaacaggctg
     5221 aagccgaagg catccccgcc tatatgattt tcggcgacaa aaccctgcgc gaccttgtcg
     5281 aaaaaatgcc gcaaaacctc aacgggctgc acgacatcta cggcttgggc gaagccaaaa
     5341 ccgaacgttt cggacacggc atactcaaag tctgccaaaa cgctgccgac tttagccacg
     5401 atgccgtcat ccgtccgcaa accgaacgcg aacaacaact gcgtcaaaaa ctcgaagcct
     5461 ggcggtatga acaggcaagg gcggaaaact gcgccctgca taccgtcctc tccgacgaaa
     5521 gccttgccga tatgcttgcc gccacgcccg aaaccgaaac cgacctcgaa gccgtacacg
     5581 gcttgggcag cgtacgcgcc gccaaatacg gacgggacat cctcgccgtc tgccgtccgt
     5641 tttcagacgg catcgatgaa accgccaaac acaaacgctg cctgatgcgc gccctgattc
     5701 aatggtgcaa cgaaaccgca aaacacgaac agtccgaacc ctaccgcatt ctcagcaaag
     5761 ccgccctgcg cgccattgcc gccaaacagc cggaaggttt ggcggagctt gccgccgtat
     5821 acggcgtagg cgaagaaaaa gccgcacgtt acggtgcggc ggtgttggcg gtgttggaac
     5881 gggatgccgt ctgaagcccg ttaccaagtt tcagacggca ttgcctctgt ttaaaaattc
     5941 ctgtttttat caaatatttc aaacagtttt tccttgtcgg cacaagaccc atccggtatc
     6001 aggtccaata tcgtttccaa cgcctcggca atatcttccc gctctaccgt gtcgataaag
     6061 tgttttttgt ccattttgtt gaaacctccg gtataggctt tgaccgcctc tgccagcccc
     6121 tcccctgtgt tttcaggcgg attgccgagc agtttgacca cgccggcacg ggttttccgg
     6181 tacagctccg ccgctttttt ggcatggctt ttcgggatat gctccgcgcc gtcccaatcg
     6241 cggaaggggt tgtcgaaatt ttgcgccagc cattcgggtt ttcgggcttt ttctatccac
     6301 aggtccagcc cgtcttcctt ccgtttctta taaagtttct tcactgcctt cgccgcctct
     6361 tcgggcaggc tgctcatcca cagcctgtgc agcttcggca gccggtcggg atgagggatg
     6421 tcgtccgcgc cgaaaccgaa caaatctacg gcagtgaaca cttccaaatc ctcaaatccc
     6481 gatacggcgg aaaaattggc gatattgccc ggtttgcccc acagccgtat ggttttgagc
     6541 ttgggatacg tttccgacag gttttgcata tcgaaatcgg caatgcccat gacgttgacg
     6601 gcggtcaggt tttccaaacc ccgcactttg ggaacgcttt tgcccacttc caaaatcaga
     6661 cccgcgccct ttccggcggt acagaccttg cagttttcct tgatttcgcc tttcaggttc
     6721 aggctgtcca agccttcgtt cagataaagc gattccacgc cggtcatatc gacagaaagg
     6781 tcggttatgc gcgtattgct gaaatccagc acggtctgac cgtggttttc cagacacaat
     6841 cgggtgataa acggatattc ctccagatat tcatatagat ttttatgcca cttggtcaga
     6901 aacagcgaag acaggcaggg aaacgccttc agttccatcg cgtcttcaaa atcatcccaa
     6961 acatcgctca aattctgttt ggatatgccg tattcccgtc cggcaaacat cacggtattt
     7021 ttgcttttgg cggctttttt gaatgccttt ctctgttttt cgggtatctg ccgccatctc
     7081 aactgacggt acacgtcgta gccgtcgccc caaaaagagg cataccgctg cgtgtcttcg
     7141 ccgcacagcg gcgcggtatt cccaacccgt acatatcccg tcggcacgac ggcacttgcc
     7201 ttatacatgt gcagaccgcg ttcccaatac ataaagtctt tgtaaagcgg tttcaaatcc
     7261 gccagctccg ccgccccaag cggtttgttg ccccgccagt ccaaagccag cagggtcgca
     7321 tgtgtttttt tgccaccgtg ttcttcaact cgggtaacct ggcaggcagt gtactgcttc
     7381 agtctgatgt tatagacgca atatacgtct ccggcatcgg cacatagtgg attaaattta
     7441 aatcaggaca aggcggcgaa gccgcggaca gtacagatag tacggcaagg cgagccaacg
     7501 ccgtaccggt ttaaatttaa ttcactatac atcggcattt cctttatttc tgtcggtttt
     7561 cacagacaaa tgccgtctga aacttggaaa cggcttcaga cgtcatctgc acatcatctc
     7621 ttcggaaaaa aacgcaccac gttctccccc tgcggcagca ccagtttttt cacggcgtgt
     7681 ccgtacaccg tttccagcgt aatgcccaaa ccgccttccc gttcaaatat cgccccgaca
     7741 cacgaacgcg cggcgttttc gtcgtcccac gccaccgccc gccaaacgcc cagcgtgtcc
     7801 atatccgccc aaaacgtttc cgcctttttg tagtccaaca ccagcttcgc cgtatcggta
     7861 actggatcgt aaaagccgtt ttcggcgagc atatcgccca agtcgtgcat atcgggaaac
     7921 atcacgctgc ggctttcaat gccgttttct ttcagacggg atttcagttc cgccaaggta
     7981 tcgcgcccga agcaggtgaa aaacagcagc ccgtccgtct tcagcgcgcg cgcccagttg
     8041 tgcagcacgg gaaggatttg ttccgccgcc aacagtccga ggttcgacca caacatatcg
     8101 gcacacgctt cgggcagcgg cacggtcggg gattggcagt gctgcaccac gcccctgccc
     8161 gtcagttttt gccagaaacc gcccttgcgc gcggctgcgg cagccgccaa aaaatccgca
     8221 cgggaatcgt attcttcaaa taccgccggc ggatagcgct tcgccaacag gctgcggctg
     8281 atgtccgcat ccgcaccggc aagcaggata tgcccgggcg cgttgcggac gagtgtcagg
     8341 cgttggtcgg tatgttcggc aagatggcgg tgaacctgcc agcatttgtc ctgcggattc
     8401 atgtcaaacc cccttcgaca aagtcgcggt acagcgcggc aaacacttcc gcatggctca
     8461 aaaacggcgc gtgtgccgcc ttttccatca caaccagcct gcttcctttc aaatgccgat
     8521 gcagatactc gcccatacgc ggcggtgtaa tcgcgtcttt gccgccgaac accagcagta
     8581 ccggaacatc tatcttgtcc aacaaatgcc gcacatccgc cctttccgcc gcgtccaaag
     8641 cttcctgtaa ggcttgaggc gtgccgcagc gcgccaaatc gggcaggatt ctgccgatga
     8701 tttcggcggc atcaggcgtg tgcagaagct ggagttgtag aaactgtttg atatgtttgg
     8761 cataatccgt tcggaacgcg ccgaccattt tgcccagtgc aggcgcggca agcccttcgg
     8821 gatagtcttc ggcagccgtc agccgtgcga aactcgccgt caggcagagc gaacggactt
     8881 tgtcgggatg gcgcgccgcc agatacagcg cgaccaatcc gccgagtgac cagccgagaa
     8941 tgtcggccga cgtatcgatt tgagcggcaa tgccgtcggc agctgcctca atatcgaagg
     9001 gttgcgcgaa gggcgcgtcc ccgtgtccgg gcaaatcgac ggcggacacc ggccacgttg
     9061 cgggcaggcg cggcatcaaa tcgtcgaacg cgtggcggtt cgccccccaa ccgtgtatca
     9121 ggtaaacttt tttggcagca tcaggcatga attttctctc ttgttggcgg cgcatcgcag
     9181 acgcgcccac tatcaggcgt tgcgtattat gccacggttc gtccggcgtt tcagacggca
     9241 tctgcgccgg ctgcaatacc gatttggcaa gtttccgcac cgacgcggca aacagctgtc
     9301 ccctgtgttt caggcacgtt caaggcggcg cggtgtgcgg cggatgtcag aaaaaaccgc
     9361 ccgcattcga ccggatgtgg gcatcgctgc attacgaacc gcccgtcagc aatatgatac
     9421 gcgcgctgaa gcacttggct gatttgggca tggcgcagcc gctggcagac ctgacgatgc
     9481 agaatccgcc cgaccggctt tcagacgaat gtttcgattt cgtcctaccc gttccgctaa
     9541 gcagggagcg gctgctgcaa cgcgggttca accaaagcga gagcatcgtc gggttgttgg
     9601 cgcaacgcta cggctggcag atactgcccc gacacaccgt attccgacac caccgcccgc
     9661 cgcaaagcac gctcaaaggc ggcgaacggc ggcgaaacat caaaaacgcc tttgaaatcc
     9721 gcacaccgat accggaaaac tgtaatattt tgttaatcga cgatgtcttt accaccggcg
     9781 cgacgctgga cgaattggca aagacgctga aaaaatcggg cgcaaaccga atctgctgct
     9841 ggacgctggc acgcacgcca atgaaaaaat aactaaattt tttgacaccc catccgcctt
     9901 atggcaaggt tacgcgccta taagtgattg atatttatgc ttaccatcgt tttatatcag
     9961 ccggaaatcc ccccgaacac gggcaacatc atccgcctgt gcgccaatac cggcgcggat
    10021 ttgcaccttg tcaaaccgct cggcttccca ttggattccg ccaaaatgaa acgcgccggg
    10081 ctcgactacc acgaattcgc cagcctgacg gtgcacgaaa acttcgacga ctgcctcaag
    10141 tcgctggcag gccgacgtat tttcgccctg accaccaaag gcacggcgcg ccccgatgaa
    10201 accgcgtttc aaaaaggcga cgttttactg ttcgggccgg aaacgcgcgg gctgcctgcc
    10261 gacatcctcg acagcctgcc cgccgcgcaa aaaatccgcc tgccgatgcg gcccggcagc
    10321 cggagtatga acctttccaa caccgtctcc gtgattctct ttgaagcgtg gcggcaacac
    10381 ggttacgcag gcggcgtttg aacgcaggtt catgccgtct gaaaaccgtt cggacacatt
    10441 ccgaaccgct gccgcagcgt gcggcggttc ggaacgcgtc cgtagggcat aatgcccatc
    10501 cgtcttcaac tcaagaacgg aacacatttt gactttaacc cgaaaaaccc ttttcctcct
    10561 caccgccgcg ttcggcacac actcccttca gacggcatcc gccgacgcag tggtcaaggc
    10621 ggaaaaactg cacgcctccg ccaaccgcag ctacaaagtc gccgaattca cgcaaaccgg
    10681 caacgcctcg tggtacggcg gcaggtttca cgggcgcaaa acttccggcg gagaccgcta
    10741 cgatatgaac gcctttaccg ccgcccacaa aaccctgccc atccccagcc atgtgcgcgt
    10801 aaccaacacc aaaaacggca aaagcgtcat cgtccgcgtc aacgaccgcg gccccttcca
    10861 cggcaaccgc atcatcgacg tatccaaagc cgccgcgcaa aaattgggct ttgtcagcca
    10921 agggacggca cacgtcaaaa tcgaacaaat cgtcccgggc caatccgcac cggttgccga
    10981 aaacaaagac atctttatcg acttgaaatc tttcggtacg gaacacgaag cacaagccta
    11041 tctgaaccaa gccgcccaaa atttcgccgc ttcgtcatca agcccgaacc tctcggttga
    11101 aaaacgccgt tacgaatacg ttgtcaaaat gggcccgttt gcctcgcagg aacgcgccgc
    11161 cgaagccgaa gcgcaggcac gcggtatggt tcgggcggta ctgacctccg gttgacggtt
    11221 attcgatacc ttattaatat ccccattttt caacccctga tttacaaggg caaatatgaa
    11281 catcaaacac cttctcttga ccgccgccgc aaccgcactg ttgggcattt ccgcccccgc
    11341 actcgcccac cacgacggac acggcgatga cgaccacgga cacgccgcac accaacacgg
    11401 caaacaagac aaaatcatca gccgcgccca agccgaaaaa gcggcttggg cgcgtgtcgg
    11461 cggcaaaatc accgacatcg atctcgaaca cgacgacggc cgtccgcact atgatgtcga
    11521 aatcgtcaaa aacggacagg aatacaaagt cgttgtcgat gcccgtaccg gccgcgtgat
    11581 ttcctcccgc cgcgacgact gaatttgata caatccgtgc cgtctggagc ccgaaccggt
    11641 ttcagacggc attttgcacc cgacacttca ggattcggca cacatgatca gcagactgac
    11701 cggcaaactg gttgaaaaaa accctccgca aatcgtcatc gatgtcaacg gtgtcggtta
    11761 tgaagccgac gtatcgatgc agaccttcta caacctgccg cccgtgggtg aaagcgtaca
    11821 actgtttacc cagcttatca ttcgggaaga cgcacatctt ttatttggtt ttgccactgc
    11881 ggaagaacgc aagaccttcc gccaactgat taaggtcggc ggcatcggcg cgaaaacggc
    11941 tttgggcatt ttgtcggcga tgacggcaga cgagctggcg cgggcggttg cagaagaaga
    12001 tgtcaaacgc ctctcctccg ctccgggaat cggcaaaaaa accgccgaac gtatggtctt
    12061 ggaactgcgc ggcaagctgg tcgcgcatac ggtaacggac gggctgtttg ccgcctcacc
    12121 cgccgccgac gaaacggaag acatcgtcag cacgctgctt gcgctgggtt acaacgaacg
    12181 cgaggcaaaa gcggcggtca aaggcgttcc gaaggggacg gacgtgggcg aaggcgtgcg
    12241 ccttgccctg aaaaacctgc tgaaataatg ccgtccgaag gcggcgcggc gtttgccctg
    12301 acgaaaccct gcgtttccgc cccgcgctgt cttcccgacg gcttttcccg taaaatggcg
    12361 ttattgtccc tcctttcgga cggcattgtg tttgatgccg tctgaaactg ttttaccgaa
    12421 atcgaaaaca atgttgaaca aaatattttc ctggttcgag tcccgaatcg acccctaccc
    12481 cgaagccgcg ccgaaaacgc ccgaaaaagg cttgtgccgg tttgtctgga gcagtatgga
    12541 cggggtgcgg aaatggatag ccgccctagc tgcgctgacc gccggcatcg gcattatgga
    12601 agccctgatt tttcaattta tgggcaaaat cgtagagtgg ctcggcaaat acgcgcccgc
    12661 cgaactgttt gccgaaaaag gttgggaact ggcggcaatg gcggcgatga tggcgttttc
    12721 ggtcgtgtgg gcgtttgccg cgtccaacgt gcgcctgcaa acccttcagg gcgtgttccc
    12781 tatgcgcctg cgctggaact tccaccgcct gatgctgaac cagagcctcg gtttttatca
    12841 ggacgaattt gccggccgcg tgtccgccaa agtcatgcag accgcgctgg cgttgcgcga
    12901 cgcggtgatg acggttgccg atatggtcgt ttatgtgtcg gtgtatttca tcacttcggg
    12961 cgtgattctc gcctcgctcg actcatggct gctgctgccc tttatcggct ggataatcgg
    13021 tttcgcttcg gtaatgcgcc tgctgattcc cagattgggg caaaccgccg cacggcaggc
    13081 gaatgcccgc tcgctgatga ccggccgcat taccgatgcc tattccaata tcgccaccgt
    13141 caaactcttt ttccacggcg cgcgcgaagc cgtctatgcc aagcagtcga tggaagaatt
    13201 tatggttacg gtgcgcgccc aaatgcggct ggcgactctg ctgcattcgt gcagcttcat
    13261 cgtcaacact tcgctgaccc tctccgccgc cgcactgggc atctggcttt ggcacaacgg
    13321 acaggtcggc gtgggtgcgg tcgccaccgc caccgcgatg gcgttgcgcg tcaacgggct
    13381 gtcgcaatac attatgtggg aatccgcgcg gctgtttgaa aacatcggca tcgtcaacga
    13441 cggcatggcg accctgtcca aaccgcacac catcctcgac aagccccaag ccctgccgct
    13501 gaacgtgccg caaggcgcaa tcaagttcga acacgtcgat ttttgctacg aagccggcaa
    13561 accgctgctc aacggcttta acctgaatat caaacccggc gaaaaagtcg gcttgatcgg
    13621 acgcagcggc gcgggcaaat ccaccatcgt caacctgctt ttgcgtttct acgaaccgca
    13681 aagcggcacg gtttcgatcg acgggcagga cataagcggc gttacccaag aatctttacg
    13741 cgcccaaatc ggtttggtca cacaagatac ctcgctgctg caccgttccg tgcgcgacaa
    13801 cattatttac ggccgccccg acgcgaccga tgccgaaatg gtttccgccg ccgaacgcgc
    13861 cgaagccgcc ggcttcatcc ccgacctttc cgatgccaaa gggcggagcg gctacgacgc
    13921 gcacgtcggc gaacgcggcg tgaaactctc cggcggacaa cgccagcgca tcgccatcgc
    13981 ccgcgtgatg ctcaaagacg cacccatcct gctgcttgac gaagccacca gcgcgctcga
    14041 ttccgaagtt gaagccgcca tccaagaaag cctcgacaaa atgatggaag gcaaaaccgt
    14101 catcgccatc gcccaccgcc tctccaccat tgccgcgatg gacaggctcg tcgtcctcga
    14161 caaaggccgc atcatcgaag aaggcacaca cgccgaactc ctcgaaaaac gcgggcttta
    14221 cgccaaactc tgggcgcacc agagcggcgg cttcctcagc gaacacgtcg agtggcagca
    14281 cgactgaacc gatgccgtcc gaacacccct tttcagacgg catttccaca cccaacccca
    14341 aagaaaccat gaacgacacc gcccaaatta ccgccggcta cggccgccgc tacatcgtcc
    14401 gcacgcccga cggcacaacc tacgaagcca gcacccgcaa aaaacgcgtc gatttcgcct
    14461 gcggcgaccg cgtccgcatc agccccgtca acgccgaaca agttgtgatt gaagattttt
    14521 taccgcgcca aagcctgctc taccgccaag acgcgtggaa aaccaaactc atcgccgcca
    14581 acgttaccca actcctcatc gtaaccgccg ccgtcccgag tccgagcgtg cggctgctac
    14641 agcgcgccct gcttgccgcc gaagccgccg gcatccgcgc cgtcatcgtc ctgaacaaag
    14701 ccgacctgcc cgaaaccgcc ctctggctcg aaaaactcaa attctacgaa acgctgggtt
    14761 accccgtcat cgaaacccgc gtgctggaaa acgccgacag cctgcgcccc gtcctgcaag
    14821 ggcacagcaa catcctgctc ggacaaagcg gtatgggcaa atccaccctg accaacgccc
    14881 ttttaggcag ccaaaccgcc cgcaccggcg acatttccgc cgcactcgac tcgggaaaac
    14941 acaccaccac ccacgcccgg ctttatgatt tgaacggcga aacccaactc atcgactccc
    15001 cgggtttgca agaatttggt ttacaccacc tccaagccgc cgatttgccg cactatttcc
    15061 ccgatttccg ccaccttgtc gggcaatgcc gcttccacaa ctgcacccac cgcgccgaac
    15121 ccggctgcgc cttcaaagcc gccgccgaaa ccggggcggc aagccccgaa cgcctcgcct
    15181 ttttgcaggg catcaccgac gaactgctcg ggtaacgcct tgccgaccgg ggcggaaaaa
    15241 atgccgtctg aagccggatt cgggtttcag acggcatccg ttttgcaaaa atgctacaat
    15301 ccgcttttta ccggaacacc cgaaactatg aagaaaaaca ccccgaaatc gtttgaagag
    15361 gccttgtcgc gccttgaatc gctgacgcag tccatgcagg gcgaaatgcc cttggaagac
    15421 gcgcttgccg cctatcagga aggcaacgag ctggtcaggt actgccaaac caagctggcc
    15481 caagtcgaac agaaattaca ggttttggat gcggacggga cgaaggagtt gaaccttgaa
    15541 tccgacgaat gatttgaaag cgtggcaaca gagggcgcag gcgcagacag agctgctgct
    15601 ggaacgcttt ttgccgtctg gaaacgaaat cccgcacacg ctgcacgaag cgatgcgcta
    15661 tgcggctttg gacggcggca agcgtctgcg cccgatgctg gtgctggcgg cttcggaatt
    15721 gggcggggcc gtggcggatg cggtcggaca ggcaatggcg gcaatcgaaa tgatccacgt
    15781 ctattctttg gttcacgacg atatgccggc gatggacaac gacagcctgc ggcgcggcaa
    15841 accgacctgc cacatcaaat atggcgaagc caccgccctt ctgaccggcg acgctttaca
    15901 aacccaagcc ttcgacgtgt tgagccgtcc gacagaactg cccgccgcac gccagttggc
    15961 aatgttgtcg gtgttggcaa aagcgggcgg cagcgcgggt atggcgggcg gacaggcaat
    16021 cgatttggca aatgtcggca aacaaatggt tcaagccgat ttggaacgga tgcacagcct
    16081 gaaaacgggg gcgttgatcc gtgcggcggt tttattgggg gcgacggcgt gtcccgatct
    16141 gtccgacgcg gaacttgccg tattggacgc ttacgcggca aaactgggtt tggcgttcca
    16201 agtcatcgac gatgtgttgg attgtgaagc ggacacggcg actttgggca agacggcggg
    16261 caaagatgcc gacaacgaca agccgactta tgtgaaactg atgggcttgg aagcggcgcg
    16321 ctcatacgca cacaaactgg ttgccgaagc agtcgcgctg ctcgaaccct tcggcgacaa
    16381 agccctgcgc ctgcggcagt tggcagaatt tgcagtcgca cgcaaatatt aaaaccggcg
    16441 tatatcgccg actgaaacgt gccggcacag cgatgccggc ctgcacttga atgaaacaaa
    16501 tgccgtctga aagggtttca gacggcattc ctatgcttgt ttgcttgttt attccctgtt
    16561 gcgcgtgtgc caaaaatacc gccgcacgaa acacgggaag gcgaaaatga ggtacaggca
    16621 gacgacggtg gcgtaaaact cccaaccctg attgtgtacc gctcccgcac gggattcgag
    16681 gatgtaggca agagaggcgg tcagcgcgaa acctgccgcc agctcgatca ggtggtgtcc
    16741 gaaatgtttg cgcttgagcg cggccacgcc gaacagtctg gtcgtgagga agggggcgtt
    16801 ggcaaagatg agtgccaaga ccaaaaggat gtacatggat gcggtcatga tgggctttct
    16861 gtttgggcat tgtcggcggc gatgccgtct gaactgcatt gcggaacggc atatcttaac
    16921 aaaaacggca gcctctgaca cggctgccgt ttcggcggct cacagcgtgt tctccaacgc
    16981 cttggcgcac cagtcgataa cggtttgcgg cataatgccc cacagcacca acagcagcgc
    17041 gttgacgctt aagagggatt ttgcggcatt gttgccgccg gcggcgggac gggcgcggcc
    17101 ggattcgtca aaatacatga ctttgaccac gcgcaggtag tagaacgcgc caaccagcga
    17161 catcacgacg gcaaacaccg acaaccatac atagccttgt ttcaaaagcg ccataatcac
    17221 gccgaatttg gcgtaaaaac ccatcagcgg cggaatgccc gccatagaga acataaccag
    17281 cagcatcaaa aaggcaagcc atacgcggtg ttggttcaac cctgccaaat cgctgatgtt
    17341 ttcgcactcg ttgtccccgt ccgacaacac catcaacacg ccgaaccctg ccgccgccat
    17401 cagcgcgtag gtaatggcgt aatagaggcc cgccgcaaag ccgaccgcgc ccgccataaa
    17461 cgccaacagg atgaaacccg tatgcgatac ggtggaatag gcgagcatac gtttgatatt
    17521 ggtctgcatg atggcggcaa ggttgccgac cagcagcgag gcggcggcaa gcagggcaaa
    17581 catcagagac cagtcatgat gcacggttcc cagcccggta acgaggatgc ggaaagcgaa
    17641 aacgacggcg gcgattttcg gggcagtgcc gaccaaggcg gtaacagaag tgggcgcgcc
    17701 gtgatacacg tcgggcatcc acatatggaa cggtaccgcg ccgagtttga acgcaacggc
    17761 gacgacgata aacaccagac ccagtttcaa cagccattcg ttagcttctt cattgaagga
    17821 agaggcgagc acgccggcaa attccagcga accggttgcg ccgtaaacca tagaaatacc
    17881 gtagagcagc aggccggatg ccagcgcgcc cagaacaaaa tatttcaagg cggcttcggc
    17941 ggcaaaaccg gaatcgcggc gcagggcaat cagggcgtaa agggcgagcg acaagagttc
    18001 caaaccgata taggcagtta aaaaatgccc cgcgctcacc atcacgctca tgcccaagag
    18061 ggcaaacagc gacagggtgt aaaactcgcc tttgaaaata ccgcgcactt ggttgtaggg
    18121 cttggcatag acaaacagga caaacgtcaa ggcatataaa accatttttg ccaaacgcga
    18181 cataccgtcc gcaatataca tcccgttgaa cgaagacgtg ctgccctgtt cccacacggc
    18241 aagctgcacc gccgccgtaa ccgccaccgt tgccaacgcg ccgtaatgcg tccaacggca
    18301 tttgtcatca ctggcccaca agtccgccgg caacaataac accagtagcg acagcagcgc
    18361 gacttcgggc agtgcgggca ttaaattcaa atctgaccaa ttcatttaca cacctcaaat
    18421 cttgctttgc gccacatggg caatcaaatc gtttgccgcc tgatgcacca cttcgataaa
    18481 tgcgttcgga tacaggccca tccccaaaac agcaaccgcc aaaaccgcca gaatcgcaaa
    18541 ctcgcggcaa ttgatgtctt tcatttcggc aacgtgcgga ttgtgaatcg cgccgaaaat
    18601 cacgcgcttg tacatccaca gggtataaga cgcgccgtaa atcagcgtca tagccgccag
    18661 cgcgcccacc cagaaattca ctttaaccgc gcccataacc accataaact cgcccacgaa
    18721 gccggaagtc gcaggcaggc cggcgttcgc cataccgaac aacatcataa acgccgcaaa
    18781 cttgggcatc acattgacca cgccgccgta atcggcaata ttgcgcgtgt gcaggcggtc
    18841 gtacatcaca ccgatgcaca taaacatcgc cgccgacacg aagccgtgcg aaatcatctg
    18901 aatgaccgcg cctttcaacg cccagtcgtc caactgcccg tcaacaaaca aaaacatccc
    18961 aagcgttaca aaacccatat ggctgatgga cgaatacgcc accagtttct tcatatcggt
    19021 ttgcaccagg gccaccatac cgatataaat tacggcaatc aggctcaata cgatgatcac
    19081 gggggcaaaa tagcgagccg cgtccggcat aatcggcagg ataaagcgca agaaaccata
    19141 cgcacccagt ttcagcgtaa ttgccgccaa caccatcgaa ccgccggtcg gcgcttcaac
    19201 gtgggcatcc ggcagccaag tgtgtacggg gaacatcggc acttttacgg caaatgacag
    19261 gaagaacgct acaaacaaaa gctgttgtac gcccaacgga atctgtttga tgttttggaa
    19321 atcgacaata gagaagctgc ctgtctgata gtacaggtaa accatggcaa ccaacatcaa
    19381 gagcgaaccc gtcagcgtat agaggaacag cttgaccgac gcatagacgc ggcgcgggcc
    19441 gccccataca ccgataatca ggtacagcgg aatcagcata ccctcgaaga acacataaaa
    19501 cagaatcgca tcctgcgcgg caaacgcgcc gttaatcaaa cccgacatca tcaggaatgc
    19561 cgccatatac tgcgccggac gtttctgaat gacttcccaa ccggccaata ccaccagcag
    19621 cgtaataaac gcattcaaga tgataaagag cactgaaata ccgtccacgc ccaatgcgta
    19681 gttgattttc agaagcggaa tccactcgtg gaactcggta aattgatagc cgccgctcaa
    19741 acggtcgaaa ccggtaaaca ggggcagcgt taccaagaaa ccggcaagcg ctcccatgaa
    19801 ggcaagcaca cgcgccagcg gcgcacggct gtctttaccg gtcgccaaaa ccagcacgcc
    19861 tgcggcgatg ggtatccata ttgccaagct gagtaggtag ttggaaaaca tagtggttaa
    19921 cctgtggtta aaataaaaaa tatagttgta tgggttggtc tttacagaca gcgttttagg
    19981 aagtcgtctg aaagaaggaa tgaatactct ccccccttct ttacttattc ccaatcaata
    20041 tctgtacgat tgcgatattc tgcctgtact aaaacatcta attcagccaa atcttttatt
    20101 tctgactgca tagctagtaa agctcttaac ttcatactta aatcaatata tttggagcct
    20161 tcgcttgctc cttttgcaga cttaaattta ttttcttcta aaaatttttt aatgggagca
    20221 tttaaaacag gatatctatc tggatattct tgacaaagca tttcagaaaa aactgaacca
    20281 cgagtggaaa ccccacattc ttttagccaa tcaatttgct tggcaaccac attatctctc
    20341 tcagtaagtg gagcatcaaa aactgattgg atggcaatgc ataattttct aaaatctgaa
    20401 gacttacccg tccttttcca cgttttatct tgaaaacgat tgcctacccc ttctccttta
    20461 ttttcgcttt tccaagacca taatttatta aattccccat aaaaatcatt attgtctatt
    20521 tttcctgctg cacattgctt aataagattt tttaattgat tacacacagt ttgatggttt
    20581 ctcatctgct ttctccgtgc cgcaatcagt tcttgattat aatttggcat ctccataaat
    20641 aacttatcca tcactgattg tctaacagtt gatttcttat agttaatttc agcttcctga
    20701 tactcctcaa gccaatcttc cgataccggg atagatttca tcgcaatttc atcagcccaa
    20761 gatttgactt ttataaaatc ctgctcggaa attttagtca aaatatttgc ttcataattg
    20821 ctattaaacg cagcatgagt caaatttgaa gaaccgatta aagcataata ttcattctta
    20881 tcattcttcc aaaatacagc tttaggatga aaaccctgaa tgccttgagc taccatcagt
    20941 gtgaatttca aatgggacgg aagccatttc aacactttcc gaatagcctc ttttttagac
    21001 aatccgaaat cttgtccaaa aataaattta aactcttgca atttgctatt gagcttattt
    21061 tttacatccc aatgagtgag atacgcagag aaaatataca gctctactgc ctctgaaaca
    21121 gctttctcat atattgaaat cagttccttc tcatttggtg tatctgaatg taaaaataac
    21181 tgtgtcatcc cttttgactc ctctatattt gttgagattg cctaaaaaat ttaggaaaca
    21241 gataatttgc atgttggcta tttcacgcct gttgggtacc attttgttcc accctttgac
    21301 ttaatgccca cttctcaaac ttttcagacg acctctacaa aactatgaac aggtcgtctg
    21361 aaactccgcc tttatctgaa caagccccag aaggtcatgc cgagcaatac caatacgccg
    21421 aacaccatag cggcggcgta ggtgtagata aagccggttt ggactttacg cacttgcgcg
    21481 gcaattgcgc cgaccagttt ggcggagccg ttgacgatgc cgttgtcgat aatggcggta
    21541 tcgccgactt tccagaagaa gtttcccaat gcgcgcgtgc ctttggcgaa gacgttgaaa
    21601 tacaaggcgt cgaggtagta tttgttttca aacaaaacgt aaaccggacg gaacgcctgc
    21661 gcgattttag cgggcaggtg cggcagtttg acgtacaaaa gccatgcgct caatacgcct
    21721 gcggtagcga gatagaggac gggcgaatgc agactgtgcg acaccatcgc caatgcgccg
    21781 tggaactctt ccttcatgat gtgcatggtc ggatgcgcgt cggcgttgac gaaaatcacg
    21841 tctttgaaga aatcgccgta gagcatgggt tcgatggcga tgtagccgat gatgacggac
    21901 ggaatcgcaa gcagaatcag cggcagggta acgaccagcg ggctttcgtg cggattgtcg
    21961 tttttgccca agccgtgatg ttcttcacca tggccgtctg aatggtgttc gggcagactg
    22021 cgccattttt cttcgccgtg gaacaccata aagtattggc ggaacgcgta aaacgcggta
    22081 acgaacacgc tggcgaggac ggcaaaatag gcaaagccgc tgcccggcag ggtgctgtat
    22141 ttcactgctt cgataatcga atctttggag tagaagccgg agaagaacgg cgtaccgatc
    22201 agagacaaat tgccgatcag catagtcagc caagtaatcg gcatgtattt tttcaggttg
    22261 cccatatggc gcatatcttg atcgtggtgc ataccgataa tcgcactgcc cgccgccaag
    22321 aacagcaagg ctttaaagaa ggcgtgggtc atgacgtgga acatcgccac agaataggcg
    22381 gacgcgccca gagccacggt catgtagccc agttgcgaca gggtagaata cgcaaccaca
    22441 cgtttgatgt cgttttgaat cacacccaag aagcccataa acagggcggt aatcgcgccg
    22501 attaccataa tgaccgacag cgcggtgctg ctcatctcat aaatcggcga catacgcgac
    22561 accataaaca aaccggcggt aaccatcgtc gcggcgtgaa tcaatgcaga aatcggagtc
    22621 gggccttcca tcgaatcggg cagccagacg tgcagcggga attgtgccga tttacccatc
    22681 gcaccgacaa acaggagcaa acaggttacg gtaatcaaag accattccac gccggggaaa
    22741 agctggatag tggcattttg tacgttgggc agataggcga atacgtcctg ataacgcagg
    22801 ctgccgccga aataggcaag caccaagccg ataccgagca aaaagccgaa gtcgccgaca
    22861 cggttgatca aaaaggcttt caggttggca aatgtcgcgc ccgggcgttt gaaatagaaa
    22921 ccgatcaaga gatacgacac caagcccacc gcttcccaac cgaagaagag ctggatgaag
    22981 ttgttgctca taatcagcat caacatgctg aatgtaaaca aagaaatata gctgaagaag
    23041 cgttggtagc cgactttttc atcgtgcata taaccgatgg tatagatatg caccatcaac
    23101 gacacgcccg ttaccacgac catcatcatc gccgtcatcg tatcgaccaa gaagccgacg
    23161 gagaaatcca aaccgcccat tgtcagccag gtatagacgt tttcatcaaa cttggcacgg
    23221 ctgccattga ggaatcccca cagcacataa gccgacagca cggcagatac agcgacaccg
    23281 agtatcgtaa ccgtatgcgc gccggcacgc ccgattttgt tgccgaacaa acccgcaatc
    23341 aacgagcctg ccaacggaac aagggcaatt atcaaatata aagtcatatc gttcataatt
    23401 accctttcaa ctcgtccaaa tcggcaacat taatcgtttg tcggttgcgg tacaccagca
    23461 ccataatcgc caaaccgatg gcggattcgg cagcggcaac ggtcaatacg aagaatacga
    23521 aaatttgtcc ggcagtatcg cccaaatgtt gcgagaaggc gataaagttg aagttcaccg
    23581 ccaaaagcat cagctcgatg gacatcagca ataccagcac gtttttgcgg ttcataaaga
    23641 tgcccatcgc gctgataccg aacaggagag cgcccaatac cagataatgc gtcaaagtaa
    23701 tcatgctttg ccctcccctt ccgtcttgag gccgtctgaa acttcgcttt cttcggcaga
    23761 ttcgacttgc ggtttgaccg cttccatttt caccagacgc atacggcctt gatcggcgcg
    23821 tactttaact tggtcggctg gatccatacg tttcggattg gtggttttgc ggtgaaccaa
    23881 tgcaatcgcg gccaccatgc ccaacagcag caataccgcc gccaattcaa acggcaacag
    23941 gtagtcggta taaatacggc tgcccaaatc gcggatattg ttgtaatcgg ccggaatgtc
    24001 tttcatcaga ccaaatgcgg ccaggtcggt tttcgggttg accagaatca ggatcagggc
    24061 aaccgccaac aacgtaccga ccacaccggc aacaggcgcg tgccgccaga aaccggcgcg
    24121 catttcctcg atgtcgatat tcagcatcat cacgacgaac aggaacaaca ccattacggc
    24181 gccgacgtaa accaccacca gcgtaacgcc caaaaactcc gcctgcatca gcatccaaat
    24241 catcgcgctc acgcagaagg tcagcaccag atgcaaagcg gcatggacag ggtttttggc
    24301 ggtaacggta cgaaccgcac cgtacaaaac gatggcggca aggatataga acagaatcac
    24361 ggaaaaagtc attcgtctgc tccttagcgg tacggcgcgt cagcggcttt gcgtttggcg
    24421 atttcggctt cgtatttgtc gccgattgcc aagaggatgg gcttggtcat atgcaagtcg
    24481 ccttttttct cgccgtggta ttcaaaaata tgggtttcca caatcgcatc ggtcgggcag
    24541 gcctcttcgc agaaaccgca gaagatgcac ttggtcaggt cgatgtcgta acgcttggtg
    24601 cggcgggtgc cgtcttcgcg ttcttccgat tcgatgttga tcgccattgc cggacacacc
    24661 gcctcgcaca atttacacgc gatgcagcgt tcctctccgt tcggataacg gcgttgcgcg
    24721 tgcagaccgc ggaaacgcac ggattgcggc gttttctctt cgggaaaata aattgtgtct
    24781 ttgcgggcaa aaaagttttt gagcgttacg cccatgcctt tgaccagttc gccaagcaga
    24841 aaggttttta ctaagttagc catattatgt tccctcaaaa cagggatttc gttaggtatt
    24901 caaaatcgct ttgttcagac ggcctcaaga tgccgtctga aacttatttc cacaaattca
    24961 gcggtgaaat catccacacg cccaaaacca cgatgtaggc gaagccgatc ggaatcagca
    25021 ctttccagcc caaacgcatg atttggtcgt agcggtagcg tgggaaggtt gcacgaatcc
    25081 acagatacca gtacagcacc gccgccattt tcacgaacat ccagaatgcg gaaggcgtac
    25141 cgacaatgcc ccagctttgc gggaacggag acaaccagcc gccgaggaac atcagcgatg
    25201 tcagcgcggc aatcagaatc atgaaaatgt attcggcaag gaagaacagc gcgaatgcaa
    25261 agccggagta ttcgacgtgg tgtccggcaa cgatttcaga ctcgccctct gccacgtcaa
    25321 acggtgcgcg gttggtttcg gcaacggcgg aaatcagata gacgatgaag attgggaaca
    25381 gcggcagcca gttccacgag aacacagaac cgcccgcgat gccttttgcc tgcgcggcaa
    25441 cgatgtcgga gaagttcatg ctgcccgata ccatcacaac gcacaccagc gcggcactca
    25501 tggcaatctc gtaggaaatg ctttgcgcgg aagcacgcat tgcacccaag aacgaatatt
    25561 tggagttgga agcccagccc gcgataatca cgccgtaaac cgacagcgag gtaatcatca
    25621 ggatgtacaa aaggccgata ttgatattgg tcagcaccca ttcttcgtta aacggaatca
    25681 ccgcccacgc cgcgaaagac ggggcgagcg acataatcgg gccgatgtag aataaggctt
    25741 tgtttgacag cttcggacgg gttacctctt taaacaagag tttgaacacg tcggcaaacg
    25801 gctgaatcag accctgcggg ccggttacgt tcgggccgac gcgaagctgc ataaagccga
    25861 tgactttacg ttcgaaatac gtcaggtagg caacggtcag aatcagcgga atcaggataa
    25921 tcacgatttt gacgatgacg gataccacca agcctacggt gatgcccaaa tcgcccagac
    25981 cgagcgttgc ggcaaagagg ttttggaacc attcctgcat aatcaagctc ccgccagttc
    26041 aatagtgccc atcaacgcac ccagcgcggc attttcggta tgcagcggca gatgcaccac
    26101 gttttcaggc aaaccggcat cggctttgac ggcaaccgat acgcttgcgc cgttttgttt
    26161 ggcgacagcg gtttgtccgt cttgcaggcc caagcgtgcc aatgtgttcg gatttacacg
    26221 cgcggcaggc acggcggcat ggctggtttc ttgcaacggt gcggaacggc gcacgataga
    26281 atcagtatga taaataccga cgccgccgac acggacgagg cggtctgagg ccgtctgaac
    26341 gccctcccct gcccatgtgc tgcggttgtc cagtttggac ggcaggcttt ccgcatccag
    26401 cgcgtctttc agaatcgcgg cggtatcgtg gtattcaaaa cctttcaggt caaacaggtt
    26461 gcccaatacg cgcaacactt tccacaacgg acgtgaatcg ccgaagcctt gtaccacgcc
    26521 gtggaaagat tgcagacggc cttccatatt gatgaagctg cctgatgttt cggtaaacgg
    26581 cgcaatcggc agcaatacgt cgcacacgtc cagcagcgtt tcgctgacaa acggcgtaaa
    26641 cgccatcacg cttttcgcct gtttcaacgc ggctacggct tttgcaccgt caaccgtatc
    26701 gatttcaggc tcgacgttga gcagcaagac tgcctgtttc ggcgtgtttg ccatttcggc
    26761 aacgctctcg cccgagttca cacccaatac gtccgcaccg acgctgttgg cggcttgcgg
    26821 caaaatgccc agcactgcgc cggtcgcgtc cgccaactct tgcgcggcgg cataaatggc
    26881 ggcgtaatca ggatggtttt gcacttccgc acccaaaatc accgctgctt tttcagcgtt
    26941 tttcaggctg gcggtaacgg catgttccgc atcggcagac aggtttttca gacggcctgc
    27001 ccactcgtcg ggatgtgcgg cttcttgaga cagaagcggc ataaacaatt cttctttact
    27061 gccggccaat acgctcaagg ccatacggtc tttggcggcg cggcgcaggc gggcggtcag
    27121 gagcggctgt tctttgcgca agttcgcacc gactaccaat acggcatcgt tgtcagccaa
    27181 agattcaata ctttgtccca accattgcgc acctttaagg ccgtctgaaa gacgtttgtc
    27241 ttgttggcgc aaacgggttg caaagttttt aacacccaag ccgtcggcaa attttttggc
    27301 cagatacagc tcttcaaccg tattcatcgg attggcccaa acaccgactt ggttttggtt
    27361 gccgtctttg gcgatacatt caatcgcgct gcggacatat tccaacgcgg ttttccaatc
    27421 cacgtccatc cactcgccac cctgtttgat tttcggattt ttcagacggc tttcgtgata
    27481 caggccttcg taggcaaaac ggtcgcggtc ggacagccag cattcgttga tggcttcgtt
    27541 ttccaacggc aacacgcggc ggacggtatg gtctttggtt tgtacaatca ggttgctgcc
    27601 caaagcgtcg tgtgcggaaa cggatttgcg gcggttcaat tcccaagtac gcgcgttgaa
    27661 gcggaacggt ttgctggtca atgcgccgac gggacacaaa tcgatgacgt tgcccgacag
    27721 ctcggtttcc accgctttgc cgataaaggg catgatttcg gagtgttcac cacgattcac
    27781 catcgcaatt tcctgcaaac cggcgatttc ttcggtgaaa cgtacgcagc gggtacagtg
    27841 gatacagcgg ctcatttcct cggcggaaac caaaggaccc atgtccttgc cgacgacgga
    27901 acgtttttct tcggtataac ggctggtggt tttgccgtag cccaccgcca aatcctgcaa
    27961 ctggcattcg ccgccctggt cgcaggtcgg acaatcaagc ggatggttga tgagcaggaa
    28021 ctccatcacg ccttcttgcg cctctcgggc ttttgccgaa tgcgtacgca caatcatgcc
    28081 gtccgttacc ggcgtggcac aggcaggcag cggtttgggg gctttttcca catccaccag
    28141 acacatacgg cagttggcgg caatagagag ctttttgtgg taacagaaat gcggaatata
    28201 agtaccgagc ttgtgcgcgg cttcaattac cgtcgcgccc tgctccacag atacctgttt
    28261 gccgtcgatt tcgatttgta acatggttcg ttcctagtta cgggtatttg ataaataatt
    28321 ttttatggag atcgtctgaa aaatgggctt cagacggcct tttgagtttc attgacaatc
    28381 aatactgttt aaaacgttta ctgccacctt gccgtcattc ccgcacaggc gggaatccat
    28441 tttttgaatt tcggcaactg cttttcaaat atcgggttct gtaaattcca ctatggattc
    28501 ccgcctgtgc gggaatgacg gcaaagttaa atttttagca ttttgctttt aaccaatata
    28561 aaaccaacta aaactataac tcatttatta tatttttaaa tgacttaaat aactctttaa
    28621 cagcacgctc attgtcttca tctgtaggct caaacttttc acaccaatta attatattta
    28681 catcgccatg caatatccta ctgcatcgca actaccaccc cgtgtccttg acgtgccaaa
    28741 ataaaatcac aattatgcga ataaagtttg tataaatttc tcctaacctc gtattcatta
    28801 tcaccagatg aataaaaacc aatcctctga ccttcatgat caaatactgc aacaaaatct
    28861 aagccgtttc catatctttc aaaaaagact agaaatttag aaaatttccg acaaatctga
    28921 ttaaaaagcg tattgagtgt cgtactctta cctttgttgg ctgcaccgta tagaataaaa
    28981 attttagctt ccaccattac caccctcccc acttatgctc tttcatcggc ccgccgtgtt
    29041 cgatgtaatg cgcaaactca tcacggaaat gcttggtaaa gctgcggaca gggaagacgg
    29101 cggcatcggc aagggcgcag atggtgcggc ctgccatttg gttgccgaca gaatccagca
    29161 aatccaagtc ttccatgcgg cctttgcctt ctacgatgcg gcggacgatg cggtaaagcc
    29221 agccggtacc ttcgcggcag ggggtgcatt ggccgcagga ttcgtcgtag tagaaatagc
    29281 ttaagcgttc gagggctttg accatgcaca cgtcttcgtc catcacgata atcgcgccgg
    29341 aaccgagcat cgagccggct ttggaaatcg agtcatagtc catattggtc tgcatcataa
    29401 tgtcggcagg caatacgggg gcggacgaac cgccgggaat aacggctttg agttttttac
    29461 cgccgcgcat accgcccgcc attttcaaga cttcggcaaa cggcgtgccc aacggcactt
    29521 catagttgcc cggacgctcg acatggccgg aaatacaaaa taatttggta ccgcctgcat
    29581 tcggaatacc tttatcggca aacgcctgtc cgccgtcacg gataatgaat ggaacggagg
    29641 agaacgtttc ggtattgttg atggtggtcg gtttgccgta caggccgaac gaggcgggga
    29701 acggcggttt gaagcgcggc tggccttttt tgccttccaa cgattcaagc aatgcggttt
    29761 cttcgccgca aatatatgcg ccgtagccgt ggtgggcgaa gagttcgaat tcaaaatccg
    29821 aacccaaaat atttttaccc aaaaagcctg cggcacgcgc ctgctccaaa gcggcttcaa
    29881 agcgttgata accttcgaaa atttcgccgt ggatatagtt gtagccggct ttcgcgccca
    29941 tcgcgtaacc ggcaatgatc atgccttcaa tcagggcatg aggattgaac atgatgatgt
    30001 cgcggtcttt aaatgtaccc ggctcgcctt cgtcggtgtt gcaaaccaca tatttttcgc
    30061 ccgggaaaga acggggcata aagctccatt tcaaaccggt cgggaagccc gcaccgccgc
    30121 gcccgcgcaa gccggaggtt ttgacttcgt caatcacatc ggtttgcgag atgttttcgg
    30181 acaggatttt acgcagggcg gtatagccgc cgcgtttgac gtattcgtcc aatgtccagc
    30241 aatcgggatt ggcggtatcc acttggtcaa aaatcacgcc tgattggtaa atagccattt
    30301 ttggtgtgcc tgtttgtttt cgtatcggtt gcggccgctg tttcagacga ccttaaaatg
    30361 tctttgtgta tcggcttgta acgtcttctg aaataaaatc tagtttatca aatcgatatt
    30421 ttcagacgac cttcaatcgt ttttgttgga tctcgaccac ttgcttgtct gccgtcattc
    30481 ccgcgcaggc gggaatccat cctcaatggt aagcaatgtc ttattaaatt cagaaaccga
    30541 atcttaccgg tggattcccg cctgcgcggg aatgacggca tttcagtatt tcagtagggc
    30601 ggattcttaa atccgacatt ttgccttttt acccactctg tcggatacaa gtatccgacc
    30661 tacgtttaaa tcgtcgtttc agacgaccta ctccaactcc gccagtttct tctcaatcgc
    30721 ctcttcggtc ataaagctgc acatgctgtg gttgttgacc agcataacgg gggcatcgcc
    30781 gcacgcgccc atgcattcgc cttcgacaag ggtaaatttg ccgtcgggcg tggtttcgcc
    30841 gtagccgata ccgagttttt gtttgaggta ttcgccggtc gccataccgc cgcgcagagc
    30901 gcagggcagg ttggtacaaa cggtcagttt gtatttgccg acaggctcaa ggtcgtacat
    30961 attgtagaaa gtggcgactt catatgcctg cgccggcgtg atgccgatgt agtcggcaac
    31021 aaaagcgatg gtttcggggg cgagccagcc tttttcggtt tgggcgatac gcaacgcgcc
    31081 catgatggcg gaacggcgtt ggtcggcggg atatttcgcc aactcaatgt cgatttgttt
    31141 taaggatttt gcggataaca ttatcggtca acctccccga atacgatgtc ctgcgtaccg
    31201 atgatggcaa cgacgtcggc gagcatatgg ccttttgcca tttcgtccat gccttgcagg
    31261 tgggcgaagc cgggtgcgcg gattttcagg cggtagggtt tgtttgcgcc gtctgaaatg
    31321 atgtaaacgc cgaactcgcc tttcgggtgt tcgacagcgg tgtaggtctc gccctcggga
    31381 acgtgcatac cctcggtaaa gagtttgaaa tggtgaatca ggtcttccat acctgttttc
    31441 atttcggtac gtttgggcgg agcgaatttg tggtttgcgg taatgaccgg gccgggattg
    31501 acgcgcaacc agtcggcgca ttgtttgatg atgcgtacgg attggcgcat ttcttccata
    31561 cggcagaggt aacggtcgta gcagtcgccg ttgacgccga cggggatgtc gaaatccatt
    31621 ttgtcgtaca cttcgtaggg ctgtgtctta cgcacgtccc attccacgcc ggaaccgcga
    31681 agcatcacac cggtaaagcc tttttgcatg gcgcgctcgg gagagacaac gccgatgccg
    31741 acggtacgct gtttccagat gcggttgtcg gtcaggaggg tttcgagtgt gtcgatattt
    31801 ttggggaagc gttcgcagaa ggcatcgata aagtcaagca tcgtgccttc gcgggattcg
    31861 ttgagctgct tcaatacttt ggcattgcgg aatttgctgc tctcgtattt gggcataaag
    31921 ccgggtaggt cgcggtaaac gccgccggga cggaagtagg cggcgtgcat acgtgcgccg
    31981 gacacggctt cgtacaagtc catcagctct tcgcggtcgc ggaaggcgta gaggatggcg
    32041 gtcatcgcgc cgatgtcgaa ggcgtgcgaa ccgatgccca tcaagtgatt gaggatgcgc
    32101 gttacttcgg cgaacatcac gcggatgtat tgggcgcgga taggcacatc gataccggca
    32161 agtttttcta ccgccaagca atacgcctgc tcgttgacca tcatggaaac gtagtccaaa
    32221 cggtccatat agggcagggc ttgcagatag gttttggttt ccgccagttt ttcggtgccc
    32281 cggtgcagga ggccgatatg cggatcggca cggacgattt gttcgccgtc cagctccaaa
    32341 atcatacgca atacgccatg cgccgcaggg tgctgcgggc cgaagttgat ggtgtagttt
    32401 cttaatttat tggccaccgt agttctcctc acggacgata cgcggtgtga tttcgcgcgg
    32461 ctcaatggta acaggttggt aaatcacgcg tttttgctct tcgtcataac gcatttccac
    32521 atagccggaa atcgggaagt ctttgcggaa cggatgtccg acaaagccgt aatcggtcag
    32581 gatgcggcgc aagtcgggat ggttgttgaa catgatgccg tacaaatcga aggcttcgcg
    32641 ctcgtaccaa tccgcgctgt tgtaaatatc ggctacggat tcgactacag ggaagtcgtc
    32701 gtctgaaacc cagacgcgta cgcggatgcg ttgattgttt ttaacggaaa gcaactgact
    32761 gacaacggca aagcgtttgc cctgccatgc ttcgtttttg taagtgctgt aatcgacgcc
    32821 gcacaagtca accagaagct cgaaatgcaa atcttcatgg tcgtgcaatg cggtcatgac
    32881 tgaaatatag tgctcgggca gacactcgac ggtaatctcg cccaaagctg aaatgacttt
    32941 gcctgcctga tcgccaagca cgccgacgac ggtctcgtat aagttttgaa tgcttgccat
    33001 atcgtcctct ccttactcgt cacgcgcgat ggtggaagtg cgcttgattt tttgttggag
    33061 ctgaatcagg ccgtaaatca gagcttccgc agtcggcgga caacccggca cataaacgtc
    33121 caccggcacg acacggtcgg caccgcgcac aacggaataa gaatagtgat aatagccgcc
    33181 gccgttggca cacgaaccca tagacaatac ccagcgcggc tcggcgagct ggtcgtacac
    33241 gcgacgcagg gctggcgcca ttttattggt cagcgtacct gccacaatca tcaggtcggc
    33301 ctgacgtggg gacggacgga aaatgatacc gaaacggtca aggtcataac gcgccatacc
    33361 cgcatgcatc atttccacgg cgcagcaggc caatccgaaa gtaaccggcc acaatgaacc
    33421 ggtacgcata tagttcaaca ccgtatccgc gctggtggtg atgaaacctt ttttcaaaac
    33481 gccttctatt cccattccag cgcacctttt ttccattcgt aaacaaagcc taccgtcagg
    33541 acgacgataa acaccagcat agaccaaaag ccgtatgcgc ccaaatcttt gaacacgact
    33601 gcccacggca gcataaacgc aacctccaaa tcgaagagga tgaacaggat ggcgacgagg
    33661 taatagcgca cgtcgaactt catccttgcg ttttcaaagg cttcaaaacc gcattcgtaa
    33721 ggcgcgtctt tttcggcata gtggcgtttc gggcccaaaa tcgtgccgag caggataaac
    33781 agtacgccgg ccgcgaggcc gacgaggata aagacaaaga cgggaaaata agcggacaac
    33841 atggttacac ccaaatccgt taacaaaatt tctacaataa tttcgtattt tagcgaattt
    33901 caaaaaccat taaaaggtaa atatcggcaa aacgcccaaa aaaacccaat aaatacaatc
    33961 atgttatgat aacgattctt atttgatttt ataaggtcat ataaattttt acaccgtaat
    34021 tcgtggtata gcggattaac aaaaaccggt acggcgttgc cccgccttag ctcaaaggga
    34081 acggttccct aaggtgccca agcaccaagc gaatcggttc cgtactatcc gtactgtctg
    34141 cggcccgccg ccttgtcctg atttttgtta atccgctata tataggaata agaattcggg
    34201 acaaaatgtt taaaccattt tgtcccgact gctgtgatgc ggtttttttg aactagcagg
    34261 acgttaccta aaacctgcgt tgcctaaaac ctgcgttgcc taaaacctgc gttgcctgaa
    34321 accctttaag acggttaaat ccctctgccg tatttgtatt ctacaatcat attatcgtca
    34381 gtaatgactt ccgccccagc cgaaggttcc gtcatccgaa tcagcatacg agagacaacc
    34441 ttttgtgctg cagcatccac ggtgctgctg tcaaatacgt gcctgccgct ttccggccaa
    34501 atcaaccggg aaaggcgttg cttgagcagt tctttattag ggaaaactac cggggttgcc
    34561 gagccgacta ccatatgccc gtagcggtat gcatagggaa tactgtgtac ggcggtagca
    34621 aaagcatgcg ggctgtgcgt ggtattaaac attacaatac catccggggt aaggtggctt
    34681 tgcacctgtt ttaaaaattc cgcactcaac aggttagtgg aataggcacg ccagtaccaa
    34741 gtcgaattca tcaaaatcag gtcgaatttt tcatcaggat ggcgacgcag ccatttccta
    34801 ccgtcatcca atacaatttc aacacgtttg tcctgcaaaa gcggtgcgat ttgcggctcg
    34861 tccgcgataa ggctacggta tgccggattg atttccgcaa cgatcatcga ctgcatttcc
    34921 ggaatggcag acaagacgcg cgcccacgaa cctgtactca atccaacgac gaaaatgcgg
    34981 cgtatgccgg acttcaggga gggtggcaga taggcacgtt cgatgccgtt gacactattg
    35041 aatatatcgg tattgtatgc gccgtcgtat acattcgccc cataaacaac cttatcacca
    35101 tctctatggt aaaccgcaac aatgccgtgt ttgttttcaa tcaacctatc cggacggcca
    35161 gcaatatttt gaaagacgga atccggcagt aggaacatga ggatgccgaa cattagggaa
    35221 actgctaccg acactgcatt cagtcggaga cttttttgga acggtgtaca aaacaaaggg
    35281 acagcagcag aaatcaaaca gatgagcagg taaatctgtt gggtggacaa gaaatcaagt
    35341 atcacaaagc cgataaggac cggacccaat gcactgccgg caacgttggc gaaataaaca
    35401 ttggaaacct gtcgtccgga tttgttgcca tccgtaccca catggtgtac aagtgggaaa
    35461 atcaaccccc tgacgacggc agacagggta atgaagatac cggcgtggtg gacgaagccg
    35521 gaaaaacccg tcaacaacca cgcagcaccc aaaatcaaaa aatcggcaat acccgcccac
    35581 aagaagcact gcccgataaa gggaatatca acaaagcggc tgcggcaaat ccgtttgcca
    35641 aaatacgcgc cgacggcgat accggtcaga aaacaggcaa gagtaaatga aaatgcctga
    35701 ggcacggact gtgctgcgaa cgaaaacatc cttacccaca agacttctat acccaagctc
    35761 aataatccgc taaggaaaga aagcatataa atcaaactag tattcggttt agtgttcacc
    35821 atatccatcc tttctgtaac gcagccatac tgaagcagca atcagaaggt taaggcaggc
    35881 tgtcagcgca atggtttggg agagggtaaa aaagacgtag aaaaattcgg cggcggcaag
    35941 cgatccgagt gccgcaccca aagtgttgaa aaaatataag gtaccgatag actcgccaac
    36001 attatgtatt ttccggttaa aaaaacaggt cagcaagggc aaggtcgcgc ccatcataaa
    36061 ggtaggaagc agcaataaga ggaaattggc agcagcgatg atgggcaaat cagcctcaac
    36121 taaaagatgc cccaagccgg aaatcagacc cttgcttacc aaaccgaaca gaccgatgga
    36181 tacttcagcg atgcaaaaca gggggatgat acttgaagga aaacggtcag caatgcgtcc
    36241 gccgaaatac gcacctacac ccaagccgac cataaataca gaaataatga cagtaatcga
    36301 actcaaatcg atacctatgt ggctgaatag aagcctctgc cagctgacct ggtaaatcag
    36361 ggcgcagaag ccagaggcga aaaacaccag cgacaatcct ttcagggttt tacttgcggt
    36421 cgaattcata ttttctattt tcaaataggc ggaactataa atgaaatgta ttaatgtgta
    36481 aacatttgta ttagccgtag gaaatcaccg cattgctcaa actgcaggcc gggaaaataa
    36541 actcgtaagc ccgcaaacgg cgtatcactg ttcaaactgc tgtgcggctt catgacgtta
    36601 taaaaattaa ggggccgtcc tggacaacta ggataaactc gattttacta attgttttaa
    36661 aatggaaca
//
LOCUS       DAWTQS010000014        36014 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.014, whole genome shotgun sequence.
ACCESSION   DAWTQS010000014 DAWTQS010000000
VERSION     DAWTQS010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 36014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36014
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.014"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            277..669
                     /locus_tag="WNA25_000701"
     CDS             277..669
                     /locus_tag="WNA25_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823512.1"
                     /translation="MQKKRHSRRHLFIPTSSKHISGLNIHSIPVLKGTGSHSVKKSGM
                     SSAQQANYPQNLFPTKNNPQVCTKGNPPYRHPFKTAIQMILKIKKSYPQKIPHINIII
                     VLLFKPYILFITLQGNGTAKRKGIFSKN"
     gene            749..2197
                     /gene="gnd"
                     /locus_tag="WNA25_000702"
     CDS             749..2197
                     /gene="gnd"
                     /locus_tag="WNA25_000702"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019269816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HFA9823513.1"
                     /translation="MKGDIGVIGLAVMGQNLILNMNDCGFKVVAYNRTIGKVDEFLNG
                     AAKETGIIGAYSLQDLVDKLAKPRKIMMMVRAGSVVDDFVEQLLPLLEEGDIIIDGGN
                     ANYPDTTRRTHYLAGKGILFVGAGVSGGEEGARRGPSIMPGGDKRAWEAVKPIFQAIA
                     AKTPQGEPCCDWVGKDGAGHFVKMVHNGIEYGDMQLICEAYQFMKDGLGLSYDEMHRV
                     FAEWNKTELDSYLIEITAAILGYKDEGGEPLAEKILDTAGQKGTGKWTGINALDLGIP
                     LTLISEAVFARCVSSFKEQRVQTGRLFARTATPVEGGKQEWVEALRQALLASKIISYA
                     QGFMLIREAGESYGWDLDYGNTALLWREGCIIRSAFLGNIRDAYEANPDLVFLGADPY
                     FKNILENCLPAWRKVVAKAVECGIPMPCMASAITFLDGYTTERLPANLLQAQRDYFGA
                     HTYERTDKPRGEFFHTNWTGKGGDTASTTYDI"
     gene            2260..3531
                     /gene="waaA"
                     /locus_tag="WNA25_000703"
     CDS             2260..3531
                     /gene="waaA"
                     /locus_tag="WNA25_000703"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFA9823514.1"
                     /translation="MFQWLYDVLWLLAPIWIRRYLDKRSGSAPAYRAHRDERFGKPHP
                     NPVTGAVWIHAVSVGETRAAQPLIRELRRRFPDAPLLMTQMTPTGRETAQVLFPDAQC
                     RYLPYDKKTWVRQFLREHRPMFGILMETEIWPNLMKECRRAGVPLFLANARLSEKSLN
                     GYLKVRRLIRPAAASLTGCLAQTEADAARLAKLGAASVQVCGNTKYDLMPSEDMKTLA
                     GQFEKRIGGRPVAVCGSTRVYRGEDEAEKLLAAWQQYRGDALLAVVPRHPEHFQTTFE
                     TAKRFGFKVQRRSDGLPVEPDTQVWIGDSMGELYAYYLCADVAFVGGSLVGSGCQNII
                     EPLSCGVTTIFGFSTYNFSEACRHALASGAAVQVESADAWREAVEKNLSGEGGGMQMQ
                     ARVDGFIAQHRGAGARIAEAVREAVCGHRGR"
     gene            3572..4021
                     /locus_tag="WNA25_000704"
     CDS             3572..4021
                     /locus_tag="WNA25_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family copper resistance protein"
                     /protein_id="HFA9823515.1"
                     /translation="MSIYAVAHIIHLYCATAFVGGVFFEVLVLSVLHTGRVSREARRE
                     VEKAMSYRAVRVMPFAVGLLFASGIVMAANRYLPISGEPFATSFGTMLTLKILLAFSV
                     LAHFAVAVVKMARSTLTVGWSKYIHAVVFTHMLLIVFLAKAMFYISW"
     gene            4054..5028
                     /locus_tag="WNA25_000705"
     CDS             4054..5028
                     /locus_tag="WNA25_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFA9823516.1"
                     /translation="MTEYPGIGSPLFYGVFFAAVLVMIALDMFSLKKNGSHKVGIKET
                     LAWSGLWVAVSCLFAGWLYFELAGNPGYGAAAAKEKVLEFFTGYILEKSLAVDNIFVF
                     LMIFGYFKVAPQFQHRVLLYGVLGALVLRTVMIFVGAALVRQFEWILYLFGAFLLYTG
                     IHMMKPEGDEEEDLANSRLLNAVKKVVPVGTAFHGEKFFTVENGKKIATPLFLVLIMI
                     ELSDVVFAVDSIPAVFAVTTDPFIVLTSNIFAILGLRAMYFLLADVAERFIFLKYGLA
                     FVLGFIGVKMLVMHWVHIPISVSLSVVFGALGASILTSLIYTKKQPDK"
     gene            5097..5172
                     /locus_tag="WNA25_000706"
     tRNA            5097..5172
                     /locus_tag="WNA25_000706"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5130..5132,aa:Lys,seq:ttt)
     gene            5393..6646
                     /gene="murA"
                     /locus_tag="WNA25_000707"
     CDS             5393..6646
                     /gene="murA"
                     /locus_tag="WNA25_000707"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFA9823517.1"
                     /translation="MDKLKISANGPLNGEITVSGAKNAALPLMCAGLLTSGTLRLKNV
                     PMLADVATTQKLLQGMGARVLTDNISEFEINGGTVNNTCAPYELVRTMRASILVLGPT
                     LARFGEAQVSLPGGCAIGSRPVNQHLKGLEAMGAEIAIEHGYVKAKGKLKGARVAMDV
                     VTVGGTENLLMAATLAEGTTVLENCAIEPEVVDLAECLVKMGAKISGIGTSTMIVEGA
                     GELYGCEHSVVPDRIEAGTFLCAVAITGGRVVLRNAAPKTMEVVLDKLVEAGAVIEAG
                     DDWIAIDMRQRPKAVDIRTVVHPGFPTDMQAQFMALNAVAEGSCRVVETIFENRFMHV
                     PELNRMGANITTEGNTAFVQGVERLSGAVVKATDLRASASLVIAGLAARGETVVERIY
                     HLDRGYENIEKKLGSVGANIERVSG"
     gene            6727..7920
                     /locus_tag="WNA25_000708"
     CDS             6727..7920
                     /locus_tag="WNA25_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFA9823518.1"
                     /translation="MAFLKLTEQNVRGKTVLIRADMNVPFKGGKISDDTRIRASLASV
                     KYCLDNGASVIVMTHLGRPTEGEFHPEDDVAPVAAHLGGLLGKDVKVLNDWRENKPAL
                     NAGDVVMLQNVRINKGEKKNDLELGKAYAALCDVFVNDAFGTAHRAQASTEAVAQAAP
                     VACAGVLMAGELDALGKALKQPARPMVAIVAGSKVSTKLTILESLADKVDQLIVGGGI
                     ANTFLLAEGKAIGKSLAEHDLVEESKKIMAKMAAKGGSVPLPTDVVVAKAFAADAEAV
                     VKDIADVAEDEMILDIGPKSAAALAELLKAAGTVVWNGPVGVFEFDQFAGGTKALAEA
                     IAQSKAFSIAGGGDTLAAIAKFGVTEQIGYISTGGGAFLEFLEGKELPAVAALEKTRR
                     VNGLI"
     gene            complement(7972..8220)
                     /locus_tag="WNA25_000709"
     CDS             complement(7972..8220)
                     /locus_tag="WNA25_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HFA9823519.1"
                     /translation="MLTPEQVKALIAGVAKCEHIEVEGDGHHFFAVIVSSEFEGKARL
                     ARHRLIKDGLKAQLESNELHALSISVAATPAEWAAKAQ"
     gene            complement(8313..9230)
                     /gene="ftsX"
                     /locus_tag="WNA25_000710"
     CDS             complement(8313..9230)
                     /gene="ftsX"
                     /locus_tag="WNA25_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HFA9823520.1"
                     /translation="MSIIHYFSLHVESARSALKQLLRQPFGTLLTLIMLAVAMTLPLF
                     MYLGIQSGQSVLGKLNESPQITVYMETAAAQSDSDTVRSLLTRDKRLDNIRFIGKEDG
                     LAELQSNLDQNLISMLDGNPLPDVFIVTPDPATTPAQMQAIYRDITKLPMVESASMDT
                     EWVQTLYQINEFIRKILWFLSLTLGMAFVLVAHNTIRLQILSRKEEIEITKLLGAPAS
                     FIRRPFLYQAMWQSIFSAAVSLGLCGWLLSAVHPLVDAIFKPYGLNIGWRFFYVGELG
                     LVFGFVIALGVFGAWLATTQHLLCFKAKK"
     gene            complement(9227..9877)
                     /locus_tag="WNA25_000711"
     CDS             complement(9227..9877)
                     /locus_tag="WNA25_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFA9823521.1"
                     /translation="MIRFEQVSKTYPGGFEALKNVSFQINKGEMIFIAGHSGSGKSTV
                     LKLISGITKPSMGKVLFNGQDLGTLSDNQIGFMRQHIGIVFQDHKILNDRNVLQNVIL
                     PLRIIGYPPRKAEERARIAIEKVGLKGRELDDPVTLSGGEQQRLCIARAVVHQPGLLI
                     ADEPSANLDRAYALDIMELFKTFHEAGTTVIVAAHDETLMADYGHRILRLSKGRLA"
     gene            complement(10072..10554)
                     /locus_tag="WNA25_000712"
     CDS             complement(10072..10554)
                     /locus_tag="WNA25_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HFA9823522.1"
                     /translation="MKRLNLAAIALAATFAAHTASADELAGWKDNTPQNLQSLKAPVR
                     IANLWATWCGPCRKEMPAMSKWYKAQKKGSVDMVGIALDTSDNIGNFLKQTPVSYPIW
                     RYTGANSRNFMKSYGNNVGVLPFTVVEAPKCGYRQTITGEVNEKSLTEAVKLAHSKCR
                     "
     gene            10678..11031
                     /gene="arsC"
                     /locus_tag="WNA25_000713"
     CDS             10678..11031
                     /gene="arsC"
                     /locus_tag="WNA25_000713"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271974.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFA9823523.1"
                     /translation="MSEIKIFHNPRCSKSRAAVSLLEERGIAAEAVKYLDTPPDLSEL
                     KDIFNKLGLESARGMMRVKDDLYKELGLDNPDLDNDALLRAIADHPALLERPIVLANG
                     KAAVGRPLENIEAVL"
     gene            11028..11684
                     /locus_tag="WNA25_000714"
     CDS             11028..11684
                     /locus_tag="WNA25_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFA9823524.1"
                     /translation="MNKRLFCSRNGLRYYLLGGFCLSVFPLLLVFASSVWAVYRTGGQ
                     VLPPYVRADAALVLGAAAWDKRPSPVFRERINHAIALYQSRRVGKIVFTGGRTKKGYM
                     TEAEVGRRYALKQGVPARNILFENTSRNTYENLNNIRPVLRANGIASVVIVSDPYHLA
                     RAAEMAEDLGVRVYMSATPTTRFDAGNKKKIFMLQEGYALSLYRLEKWGSRFLGWLSD
                     "
     gene            11800..13194
                     /locus_tag="WNA25_000715"
     CDS             11800..13194
                     /locus_tag="WNA25_000715"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFA9823525.1"
                     /translation="MTVKTRFAPSPTGYLHIGGVRTALFSWAFARHHKGEFLLRIEDT
                     DLARSTAESVNIILDGMKWVGLDYDNADNVVYQTRRFDRYKEVIAELLAKGDAYYCYC
                     SKEELEAMREKAEKEGTATYDRRWRPEAGKTLPEIPAGVQPVVRFKTPLDGVTKWTDL
                     VKGEISIPNEALDDLIIARADGTPTYNFCAVVDDYDMGVTHIIRGDDHVNNTPKQINI
                     LKAIGANLPEYGHLPMILNEQGKKISKRSGDTVAITDFGAMGILPEAMLNYLARLGWA
                     HGDDEFFTTEQFIEWFDLKDVSPSPSRMDLKKLYWINGEHIKITPDGKLTELVKPRLA
                     LRDIHETEKPALEDVLALVKDRAQDLNALADECLYFYKKQVPAEADVAKHWDDEAAAR
                     MLRFAERLEGLEDWNAKAIHDLFKPFCDEEGIKMGKLGMPLRLAVCGTAKTPSVDAVL
                     ALISKEEVLKRIRA"
     gene            13215..13610
                     /locus_tag="WNA25_000716"
     CDS             13215..13610
                     /locus_tag="WNA25_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823526.1"
                     /translation="MPSDVGMRLQTAFRRKLNMKKILYFLMFVFSTSVWAGGAEDNLL
                     SIQSGYRALLQKQNNLDGKIIGMQSDLEDARRRLQAAQADIARLEAEIPAAMAQKARQ
                     AEEMRQIGVRLDHAWNAVYGAGERKRRGN"
     gene            13614..14105
                     /locus_tag="WNA25_000717"
     CDS             13614..14105
                     /locus_tag="WNA25_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFA9823527.1"
                     /translation="MNSLFVDNTVFITRLKAGHIGRLVQALFEEWHGFEPWSSVDKIR
                     AYYGRCLKDDELPLAFAAVDDSGTLLGSAAVKRHDMENFPQYEYWLGDVFVLPEYRGK
                     GIGRRLVAHCIGAARSLGIKFLYLYTPDVQIFYESFGWAVVGRHFHNGEWVTVMRLDV
                     DKV"
     gene            complement(14381..16975)
                     /gene="mutS"
                     /locus_tag="WNA25_000718"
     CDS             complement(14381..16975)
                     /gene="mutS"
                     /locus_tag="WNA25_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFA9823528.1"
                     /translation="MSKSAVSPMMQQYLGIKAQHTDKLVFYRMGDFYELFLDDAVEAA
                     KLLDITLTTRGQMDGVPIKMAGVPFHAAEQYLARLVKLGKSVAICEQVGEVGAGKGPV
                     ERKVVRIVTPGTLTDSALLEDKETNRIVAVSPDKKYIGLAWASLQSGEFKTKLTTADK
                     LNDELARLQAAEILLPDSKNAPQLQTASGVTRLNAWQFAADAGEKLLTEYFGCQDLRG
                     FGLDSKEHAVSIGAAGALLNYIRLTQNLMPQHLDGLSLETDSQYIGMDAATRRNLEIT
                     QTLSGKKTPTLFSILDGCATHMGSRLLALWLHHPLRNRAHIRARQEAVTALESQYEPL
                     QCHLKSIADIERIAARIAVGNARPRDLASLRDSLFELAQIDLSATGSSLLETLKAVFP
                     ETLPVAETLKAAVMPEPSVWLKDGNVINHGFHPELDELRRIQNHGDEFLLDLEAKERE
                     RTGLSTLKVEFNRVHGFYIELSKTQAEQAPADYQRRQTLKNAERFITPELKAFEDKVL
                     TAQDQALALEKQLFDGVLKNLRTALPQLQKAAKAAAALDVLSTFSALAKERNFVRPEF
                     ADYPVVHIENGRHPVVEQQVRHFTANHTDLDHKHRLMLLTGPNMGGKSTYMRQVALIV
                     LLAHTGCFVPADAATIGPVDQIFTRIGASDDLASNRSTFMVEMSETAYILHHATEQSI
                     VLMDEVGRGTSTFDGLALAHAIAEHLLQKNKSFSLFATHYFELTYLPEAHAAAVNMHL
                     SALEQGRDIVFLHQIQPGPAGKSYGIAVAKLAGLPVRALKAAQKHLNGLENQAAANRP
                     QLDIFSTMPSEKGDEPNVDCFVDKAEEKHFEGILAAALENLDPDSLTPREALSELYRL
                     KDLCKSVS"
     gene            17406..18410
                     /gene="gap"
                     /locus_tag="WNA25_000719"
     CDS             17406..18410
                     /gene="gap"
                     /locus_tag="WNA25_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003766473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFA9823529.1"
                     /translation="MSIKVAINGFGRIGRLALRQIEKAHGIEVAAVNDLTPAEMLLHL
                     FKYDSTQGRFQGTAELKDDAIVVNGREIKVFANPNPEELPWGELGVDVVLECTGFFTN
                     KTKAEAHIRAGARKVVISAPGGNDVKTVVYGVNQDILDGSETVISAASCTTNCLAPMA
                     AVLQKEFGVVEGLMTTIHAYTGDQNTLDAPHRKGDLRRARAAALNIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPVATGSLTELVSVLERPATKEEINAAMKAASSESYGYNEDQ
                     IVSSDVVGIEYGSLFDATQTRVMTVGGKQLVKTVAWYDNEMSYTCQLVRTLEYFAGKI
                     "
     gene            complement(18562..19242)
                     /gene="tsaA"
                     /locus_tag="WNA25_000720"
     CDS             complement(18562..19242)
                     /gene="tsaA"
                     /locus_tag="WNA25_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFA9823530.1"
                     /translation="MTYTITPIGAARSPYKQKFGIARQPGLVSAAEACIELNPEFTAD
                     SVRGLEDFDYVWISFIFHGVLDEGWAQMVRPPRLGGKQKMGVFATRSPHRPNHLGLSL
                     LKLERIETGKPVRLYCSGSDLLDGTPIVDIKPYIPFIESKPDAASGFVSGKPVELEVV
                     WQENIGAENLSANTKNLISQSIAQDPRPAYQNIPERIYVMNIADYEVRFQIEENRATV
                     INLSPTPL"
     gene            19702..20337
                     /locus_tag="WNA25_000721"
     CDS             19702..20337
                     /locus_tag="WNA25_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HFA9823531.1"
                     /translation="MIVSIDVDAQKTFTPLCPDELPVNEGHLIVEELNAQAALADLRV
                     MTKDAHHMAAKWLVDNPVDMLKPTGFSDADLTWVAHAMVGTRGYELLDGLPSVKEYDY
                     CVWKGVDPELHPYGACFHDIEEKLSTGLIEWLRCQNTNMVIVGGLATDYCVKTTVLQL
                     LKGGRWQVIVNEAACRGIAPDTIEAAWQEMRSSGAIILKNAEKIKKYINNQ"
     gene            20374..21342
                     /gene="waaC"
                     /locus_tag="WNA25_000722"
     CDS             20374..21342
                     /gene="waaC"
                     /locus_tag="WNA25_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HFA9823532.1"
                     /translation="MKILLVRLSSMGDLIHTLPAIEDLARQCSDVELHWLCEAGFAGI
                     ARLHPFVKKVHVMKWRQWRKHLFQAETWREMGRLKQALLQEAFDFVLDSQGLIKSACF
                     AKMAKSPIYGLDKHSAREGVAALAYDKKYVVPKGRNAVWRNRDLFAQVFGYAMPETQV
                     FGLSVPEASRLKNLAQPYYAALHATSRDSKLWPMENWRELLQKLNQKQQCNVYLPWGN
                     EAEKVRAEQIADGLPFTIVCAKMNLLQAAYLLKHAVGIVGVDTGLLHLANALEKPVVG
                     IYTDTDPIKTGVQVSAIAKNVGNIGQIPTADLVYQTLMDCVSADKG"
     gene            21568..22317
                     /locus_tag="WNA25_000723"
     CDS             21568..22317
                     /locus_tag="WNA25_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFA9823533.1"
                     /translation="MKALVAVKRVVDYNVKVRVKADGSDVDIGNVKMSMNPFDEIAVE
                     EAVHLKEAGKVSEIVAVSLGEKKCEETLRTALAMGADRAIHIETDVKLEPLAVAKLLK
                     AVADKENPQIFFLGKQAIDDDANQVAQMLAALLNAAQGTFVSKVQIEGDEVQIVREID
                     GGEETIALKLPAVISADLRLNEPRFVKLPNIMAAKKKPLEKLDSADLATDISPRLKTV
                     KFAEPKARQTGVKVASVAELVEKLKNEAKVI"
     gene            22328..23263
                     /locus_tag="WNA25_000724"
     CDS             22328..23263
                     /locus_tag="WNA25_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFA9823534.1"
                     /translation="MSVLIIAEHDNKQLNPATLHAVAAAAKLGKVDLLVVGNGASSVV
                     EFAKQVAGVEKVLVADAAHYAEGLAEELAPLVVKLAADYRYVAATATTFGKNLLPRVA
                     ALLDVPQISDLTEIVDNTTFVRPIYAGNAFETVQADSEKLVLTFRVTAFDAVAAQGGN
                     AEVINVKTTPAQNLSRFVNRQLSHSDRPELTQAKVIVSGGRALGSVEKFSEVLTPLAD
                     VLGAAIGASRAAVDAEYAPNDAQVGQTGKVVAPQLYFAIGISGAIQHVAGMQDSKVIV
                     AINKDADAPIFNVADYGLVGDLFEIVPQLIELLKN"
     gene            23326..23937
                     /locus_tag="WNA25_000725"
     CDS             23326..23937
                     /locus_tag="WNA25_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFA9823535.1"
                     /translation="MITTYKTITSPTQAEFKDKGSRFIAFAYPIRTLADVKKYLNLLK
                     EEHYKARHWCYAYRLGVNGMQFRGNDDGEPPGSAGRPILGQIDSVGITDVLVVVVRYF
                     GGALLGVPGLIHAYKEATAQALAIAEVVEKNIEKTVWLKCEYPFLNEAICIAKQYQAD
                     ILEQDLQLDCRLTVSLSLVNYEACVAAWKNTRQVEVNTEKPFE"
     gene            complement(23989..24327)
                     /locus_tag="WNA25_000726"
     CDS             complement(23989..24327)
                     /locus_tag="WNA25_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HFA9823536.1"
                     /translation="MKKEQANTQKRIDEIQSKYREWCHLLLQLEENIRRWKYAVALIR
                     DMDNFYTHEYQVRHQTIEDGVELNLQTEGEYSIMSEDALWNAPGEFHQLAWLYLCSSV
                     DTLDRYTQEN"
     gene            24464..25735
                     /gene="purD"
                     /locus_tag="WNA25_000727"
     CDS             24464..25735
                     /gene="purD"
                     /locus_tag="WNA25_000727"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFA9823537.1"
                     /translation="MKLLVIGNGGREHALAWKLAQSPKVETVFVAPGNAGTAIESKLQ
                     NIALTAYQDLIEFCRKENIVFTVVGPEAPLAAGIVDDFRAAGLKIFGPTQYAAQLESS
                     KDFAKAFMVKYNIPTAQYQTFENADAAHDYVNQKGAPIVIKADGLVAGKGVIVAMTLD
                     EAHAAIDDMLLGNKMGNAGERVVIEDFLQGEEASFIVMVDGNHVLPMATSRDHKRLLD
                     GDKGPNTGGMGAYSPAPVVTPAVYERAMNEIILPTVAGMKAEGHEFTGFLYAGLMIDQ
                     SGAPYTIEFNCRFGDPETQPIMSRLNSDLADLVEAAIDGRLDSVKAEWNPQTAVGVVL
                     AAQNYPETPKKGDVISGLDDVNRIGKVFHAGTTVNEKGDVLTNGGRILCVVGLGDDVA
                     QAKAKAYGALEKISFDGMQYRKDIADKAINR"
     gene            25769..26371
                     /locus_tag="WNA25_000728"
     CDS             25769..26371
                     /locus_tag="WNA25_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFA9823538.1"
                     /translation="MTDALQTAFYIMLFSRIIAASAQRKLSVYLKKGGLVAYPTESCY
                     GLGCLPTLAKALGKLAHLKKRPQHKGMIVIGNQLEQLQPLLQMPFENIQTMLRNEWPA
                     PKTFLLLAKSGVLPALRGKRRSKLAVRVPDHTGARRLCQALGMPLVSTSCNRAGKRAC
                     RTEREVRRQFGRDVWIVGGRIGRQKSPSQIIDGETGKRLR"
     gene            26386..26901
                     /locus_tag="WNA25_000729"
     CDS             26386..26901
                     /locus_tag="WNA25_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823539.1"
                     /translation="MKTFLHTQRKNFLDGISLHDCYRTAIKVEGRNVCFDFEDGFVVL
                     DNNPNNQAGKHLKTDFSRVVLTHENRNAEDDYLVDIFEDIVFLGKRLFTVRKFLDFSE
                     LLEMINAQGYFLEFLYLYECIGVKTSDYFLEAVLVTGRSRECKKCFIKIMRASSFVYQ
                     WNNLRLNSEIV"
     gene            complement(27053..27493)
                     /locus_tag="WNA25_000730"
     CDS             complement(27053..27493)
                     /locus_tag="WNA25_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823540.1"
                     /translation="MIKETLMRPIFLSFVLLPILITACSTPDKSARWENIGTISNGNI
                     HTYINKDSVRKNGNLMIFQDKKVVTNLKQERFANTPAYKTAIAEWEIHCNNKTYRLSS
                     LQLFDTKNTEISTQNYTASSLRPMSILSGTLTEKQYETVCGKKL"
     gene            complement(27725..27904)
                     /locus_tag="WNA25_000731"
     CDS             complement(27725..27904)
                     /locus_tag="WNA25_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HFA9823541.1"
                     /translation="MKKCRDIALLLSKHQDRETTPGEKISIYMHLLFCPHCREYKRQL
                     QTIKISLAKTTRTSK"
     gene            complement(27910..28497)
                     /locus_tag="WNA25_000732"
     CDS             complement(27910..28497)
                     /locus_tag="WNA25_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFA9823542.1"
                     /translation="MPLPDLTDAELMESRKLLLHFARLQLPDHPDLAEDLVQETLLSA
                     YSAGDSFQGRALVNSWLFAILKNKIIDALRQIGRQRKVFTALDDELLDEAFESHFSQN
                     GHWTPEGQPQHWNTPEKSLNNNEFQKILQSCLYNLPENTARVFTLKEILGFSSNEIQQ
                     MCGISTSNYHTIMHRARESLRQCLQIKWFNQENPK"
     gene            complement(28519..29265)
                     /locus_tag="WNA25_000733"
     CDS             complement(28519..29265)
                     /locus_tag="WNA25_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2063 domain-containing protein"
                     /protein_id="HFA9823543.1"
                     /translation="MQPETSAQYQHRFSQAIRGGEAADGLPQDRLNVYIRLIRNNIHS
                     FIDRCYTETRQYFDSKEWSRLKEGFVRDARAQTPYFQEIPGEFLQYCQSLPLSDGILA
                     LMDFEYTQLLAEVAQIPDIPDIHYSNDSKYTPSPAAFIRQYRYDVTHDLQEAETALLI
                     WRNAEDDVMYQTLDGFDMMLLEIMGSSALSFDTLAQTLVEFMPKADNWKNILLGKWSG
                     WIEQRIIIPSLSAISENMEGNSPSQNHLSA"
     gene            complement(29255..30097)
                     /locus_tag="WNA25_000734"
     CDS             complement(29255..30097)
                     /locus_tag="WNA25_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HFA9823544.1"
                     /translation="MIQHAGLGYRRDLAEDFLSLSENSPICFIEAAPENWLKMGGRAR
                     KQFDRVAERLPLALHGLSMSLGGQAPLDTDLIDGIKEMMCRYDCTFFSDHLSYCHDGG
                     HLYDLLPLPFTEEMVHHTARRIREVQDRLGCRIAVENTSYYLHSPLAEMNEVEFLNAV
                     AREADCGIHLDVNNIYVNAVNHGLLSPEAFLENVDAGRVCYIHIAGHDAETPELLIDT
                     HGAAVLPTVWDLLELAYTKLPTIPPTLLERDFNFPPFAELEAEVAKIADYQTRAGKEY
                     RRAA"
     gene            complement(30215..30523)
                     /locus_tag="WNA25_000735"
     CDS             complement(30215..30523)
                     /locus_tag="WNA25_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823545.1"
                     /translation="MNKNIAAALAGALSLSLAAGAVAAHKPASNATGVQKSAQGSCGA
                     SKSAEGSCGAAASKAGEGKCGEGKCGATVKKAHKHTKASKAKAKSAEGKCGEGKCGSK
                     "
     gene            complement(30597..31046)
                     /locus_tag="WNA25_000736"
     CDS             complement(30597..31046)
                     /locus_tag="WNA25_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFA9823546.1"
                     /translation="MKIGTTWQTASAMLVLRLFAAYEFLESGLQKWNGENWFSEINDQ
                     FPFPFNLLPDALNWNFAMYAELLLPVLLLLGLATRLSALGLMVVTAVAWAAVHAGSGY
                     NVCDNGYKMALIYIVVLIPLLFQGAGGWSLDTLLKKLFCPKCRLKQD"
     gene            31386..32279
                     /locus_tag="WNA25_000737"
     CDS             31386..32279
                     /locus_tag="WNA25_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFA9823547.1"
                     /translation="MVTFSKIRSFLAIAAAALLAACGTAGNNAARKPVQTAKPAAVVA
                     LALGGGASKGFAHIGIVKVLKENGIPVKVVTGTSAGSIVGSLLASGMSPDRLELEAEI
                     LGKTDLVDLTLSTSGFIKGEKLQNYINRKVGGRQIQQFPIKFAAVATDFETGKAVAFN
                     QGNAGQAVRASAAIPNVFQPVIIGRHKYVDGGLSQPVPVSAARRQGANFVIAVDISAR
                     PSKNVGQGFFSYLDQTLNVMSVSVLQNELGQADVVIKPQVLDLGAVGGFDQKKRAIRL
                     GEEAARAALPEIKRKLAAYRY"
     gene            32382..33485
                     /gene="prfB"
                     /locus_tag="WNA25_000738"
     CDS             32382..33485
                     /gene="prfB"
                     /locus_tag="WNA25_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFA9823548.1"
                     /translation="MEAEVINQLNNTLNDLEKRSEDIRVYMDYQGKKDRLEEVIGLSE
                     DPELWNDPKRAQEIGKESKILEGIVLTLDNIASGIEDNRMLIEMAVEENDEEGFAAVK
                     EDVAGLEKQMADLEFKRMFNQPADPNNCFIDITAGAGGTEAEDWAGMLFRMYSRYAER
                     KGFKIEILEEDDGEIAGINRATIRVEGEYAYGLLRTETGVHRLVRYSPFDSNNKRHTS
                     FASVFVYPEIDDSIEIEINPADLRIDTYRASGAGGQHINKTDSAVRITHEPTGIVVQC
                     QNDRSQHANKAAAMEMLKSKLYELEMRKRNEEKQALEEGKSDVGWGSQIRSYVLDSSR
                     IKDLRTGYEVGNTKAVLDGDLDGFIEASLKQGV"
     gene            33564..33923
                     /gene="rnpB"
                     /locus_tag="WNA25_000739"
     ncRNA           33564..33923
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="WNA25_000739"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(34250..35707)
                     /locus_tag="WNA25_000740"
     CDS             complement(34250..35707)
                     /locus_tag="WNA25_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFA9823549.1"
                     /translation="MSRHPARTGEKTFFGHPFQLSTLFHIELWERFSFYGMQGILLIY
                     LYYTADKGGLGIDKTLAGGIVGAYSGSVYLSTILGAWFADRVWGAEKTLFLSGIVVML
                     GHIVLAAAPGLYGLLIGLIFIALGSGGVKSTASSMVGALYEQDEMRPLRDAGFSIFYI
                     AINIGGFLGPLLTGLLQENIGFHYGFGAAAVGMAFGLWRYSLGRKNLPHPTVPHPLSK
                     GQGKTAAAVGITLIAALATAIKTGLVNLDNFSGILLSTVILAVIAYFARLLTNPRVSS
                     DNKRHIIAYIPLFLTICMFWAVWFQIYTVATVYFDETVNRTIGSFTVPVAWKDSMQSL
                     WVILFSGLMAAMWTKMGRKQPKTPLKFAMAVFVTGASFLGFVPFISSGTPMPIAVFAL
                     IVLAITIGELMISPIALSISTKIAPPLFKTQMVALNFLAFSLGFTLGGVLFEKGYQAG
                     DEIGFYRLLFYIGAATGFLLLLLVPKLNKMLEGTD"
BASE COUNT         8709 a         8290 c        10137 g         8878 t
ORIGIN      
        1 tagcccgcct tttatagcgg attaacaaaa accggtacgg cgttgccccg ccccggctca
       61 aagggaacgg ttccctgaag gcgcccaagc accgggcgaa ccggttccgt accatttgta
      121 ctgcccgcgg cccgccgcct tgtcctgatc tttgttaatc cgctatgctt atacacaacc
      181 agccaaaagc catcttattg aaatatcata agatttactc aaaaatctta ttttctccac
      241 cacttatgca caaactcgga tacacactct gccaatatgc agaaaaaaag acattcgcgc
      301 cgacaccttt tcatccccac aagttccaaa cacatttccg gcttaaatat ccacagcatt
      361 cccgttctca aaggcaccgg cagccattcg gtaaagaaaa gcgggatgtc gtccgcacaa
      421 caagcaaact atccacaaaa cctattcccc acgaaaaaca atccacaggt atgcacaaaa
      481 gggaatcctc cttaccgaca cccttttaaa acggcaattc agatgatttt aaaaataaaa
      541 aaaagttacc cacagaaaat tccccatatc aatataatca tcgttttatt atttaaacct
      601 tatattttat ttataaccct tcagggaaac ggcacggcaa agcgtaaagg catcttttca
      661 aaaaattaaa aagttaagga ttgttaaaca agtgtaaatc aggtagagtt gcagccgata
      721 aattaaatct ataacggagc ttcctgcaat gaaaggcgat attggcgtaa tcggtttggc
      781 ggttatgggt caaaacctga ttttgaatat gaacgattgc ggatttaagg ttgtcgccta
      841 caaccggaca atcggtaaag tggacgaatt tttaaacggt gcggcaaaag agacgggcat
      901 tatcggcgcg tattccctgc aagacttggt cgataagctg gcaaaaccga gaaaaatcat
      961 gatgatggtt cgcgcaggtt cggtggttga cgactttgtc gaacaactgc ttccgctgtt
     1021 ggaagaaggc gacatcatta tcgacggcgg caatgccaat taccccgaca cgacacggcg
     1081 gacacattac cttgccggaa aagggatttt gtttgtcggc gcgggcgtat ccggcggcga
     1141 agaaggcgcg cggcgcgggc cgtccatcat gccgggcggg gataagcgcg cttgggaagc
     1201 ggtcaagccg attttccagg cgattgccgc caaaacgccg cagggcgaac cgtgttgcga
     1261 ctgggtcggc aaggatggcg cgggacattt tgtcaaaatg gtgcacaacg gcatcgaata
     1321 cggcgatatg cagctgattt gcgaagcgta ccagtttatg aaagacggtt tggggctgtc
     1381 ctacgacgaa atgcaccgcg tgtttgccga gtggaacaaa accgagctgg attcttatct
     1441 gattgaaatc acagcggcaa ttttgggcta taaggacgaa ggcggcgaac cgctggccga
     1501 gaaaatcctc gatacggcag ggcaaaaagg cacgggcaaa tggacgggca tcaatgcctt
     1561 ggatttgggc attccgctga cgctgatttc cgaagccgta ttcgcccgct gtgtttcgtc
     1621 gttcaaagaa cagcgcgtgc agaccggcag gctgttcgcc cgaaccgcca cgcccgtcga
     1681 aggcggcaaa caagaatggg tcgaggcgtt gagacaggct ttgcttgcct ccaaaatcat
     1741 ttcctacgca caaggcttta tgttgatccg cgaagcgggc gaaagttacg gctgggattt
     1801 ggactacggc aacactgcgc tgctgtggcg cgaaggctgc atcatccgca gcgcgttttt
     1861 gggcaatatc cgcgacgcgt atgaggccaa tcccgatttg gtgttcttgg gggcggatcc
     1921 gtatttcaaa aatattttgg aaaactgcct gccggcatgg cgcaaggtgg ttgccaaggc
     1981 ggtcgaatgc ggcatcccta tgccctgcat ggcttcggcg attacgttct tggacggcta
     2041 tacgaccgaa cgcctgcctg ccaacctctt gcaggcacag cgcgactact tcggcgcgca
     2101 cacttacgag cgcaccgaca aaccgcgcgg cgaatttttc cataccaact ggacgggcaa
     2161 gggcggagat accgcttcga caacctacga tatttgattc cgttgccgaa atgccgtccg
     2221 aaccgttcgg acggcatttg ttttgcagga aatagaaaga tgttccaatg gctttatgat
     2281 gtattgtggc tgcttgcgcc gatatggata cggcgttatt tggacaaacg ctccggaagt
     2341 gccccggcat accgggcgca tagggacgag cgtttcggca agccgcatcc gaatcccgtt
     2401 accggcgcgg tttggataca cgccgtttcg gtcggagaaa cgcgtgccgc ccagcccttg
     2461 atacgcgagt tgcggcggcg ttttcccgat gcgccgctgc tgatgacgca gatgaccccg
     2521 acgggacggg aaaccgcgca agttctgttt cccgatgcgc aatgccgcta tcttccgtat
     2581 gacaaaaaaa cgtgggtacg gcagtttttg cgcgaacacc gcccgatgtt cggcattttg
     2641 atggaaaccg aaatctggcc caatctgatg aaggaatgcc ggcgagcggg tgtgccgctg
     2701 tttttggcga atgcgcggct gtcggaaaaa tcgctgaacg gttatctgaa agtccgccgc
     2761 ctgatccgtc ccgcagccgc ttcgctgacg ggctgtctgg cgcagacgga ggcggatgcg
     2821 gcgcggttgg cgaaattggg cgcggcatcc gtgcaggtgt gcggcaatac caaatacgac
     2881 ctgatgccgt ctgaagacat gaaaacgctg gcggggcagt ttgaaaaacg catcggaggc
     2941 cggccggttg ccgtgtgcgg cagcacgcgc gtttatcggg gcgaagacga ggcggaaaaa
     3001 ctgctggcgg cgtggcaaca atatcgcggc gatgccctgc tggccgtcgt gccgcgccat
     3061 cccgagcatt ttcagacgac atttgaaacg gcgaaacgct tcgggtttaa ggttcagcgg
     3121 cgcagcgacg gtttgccggt cgaacccgat acgcaggtgt ggataggcga cagtatgggc
     3181 gagctgtatg cgtattacct gtgcgccgat gtcgcttttg tcggcggcag tctggtcggt
     3241 tcgggctgtc agaacatcat cgaaccgctt tcctgcggcg ttacgacgat attcggcttt
     3301 tcgacctaca atttttccga agcctgccgg cacgccttgg catcgggtgc ggcggttcaa
     3361 gtcgaatcgg cggacgcgtg gcgtgaagcc gttgaaaaaa acttatctgg cgaggggggc
     3421 ggaatgcaga tgcaggcgcg cgtggacggc tttatcgcac aacatcgcgg agcgggcgcg
     3481 agaatcgccg aggcggtgcg ggaagcggta tgcggacatc gggggcgata gtgatacaat
     3541 ccgtatccga gttttccggt tggagtaccg tatgagtatt tatgccgtcg cgcacatcat
     3601 ccacctgtat tgcgccaccg cctttgtcgg cggcgtgttt tttgaagtgc tggttttgtc
     3661 cgtcctgcat acgggacggg tgtcgcgcga ggcgcggcgc gaagtggaaa aggcaatgtc
     3721 ttaccgcgcc gtcagggtga tgccgtttgc ggtcggactg ctgttcgcca gcggcatcgt
     3781 gatggcggca aaccgctacc ttcctatatc gggcgaaccg tttgccactt ccttcggtac
     3841 gatgctgacg ctgaaaatcc tgttggcgtt cagcgtgttg gcgcacttcg ccgtcgccgt
     3901 cgtcaaaatg gcgcgttcca cgctgacggt cggctggtcg aaatacatac acgccgtcgt
     3961 ctttacccat atgctgctga ttgtcttttt ggcaaaagcg atgttttata tcagctggta
     4021 acacaactac atttcaaatc aggagattga accatgacag aatatcccgg catcgggtcg
     4081 ccgttgtttt acggcgtttt ttttgcggca gtgctggtca tgattgcctt ggatatgttt
     4141 tcgctgaaga aaaacggcag ccataaagtc ggcatcaaag aaaccttggc atggagcggc
     4201 ttgtgggttg ccgtatcctg cctgttcgca ggctggctgt atttcgaact cgccggcaat
     4261 cccggctacg gcgcggcagc cgcaaaagaa aaagtattgg aattctttac cggctacatt
     4321 ttggaaaaat cgttggcagt cgacaatatt ttcgtgttcc tgatgatatt cggctacttc
     4381 aaagtcgcac cgcagtttca acaccgcgtg ctgctgtacg gcgtattggg cgcattggta
     4441 ttgcgcaccg tcatgatttt cgtcggcgcg gcactggttc ggcagttcga gtggattctg
     4501 tatctgttcg gcgcgttcct gctctatacc ggcatacaca tgatgaagcc cgaaggagat
     4561 gaggaagagg atttggcaaa cagcaggctg ctgaatgccg tcaagaaagt cgttccggtc
     4621 ggcacggcgt ttcacggcga gaaatttttt accgtcgaga atggcaaaaa aatcgccacg
     4681 ccgctgtttt tggtgctgat tatgattgaa ttgagcgatg tcgtttttgc cgtggacagt
     4741 atccccgccg tctttgccgt taccaccgat ccgtttatcg tgctgacttc caatattttc
     4801 gctattttgg gtttgcgggc gatgtatttc ctgctggcgg atgtggcgga acgctttatc
     4861 ttcctgaaat acggcttggc attcgtgttg ggttttatcg gtgtgaaaat gctggtgatg
     4921 cattgggtac acatcccgat ttccgtttcg ctgtcggtcg tgttcggcgc gttgggtgca
     4981 tcgatactga cctcgttaat ttatacgaaa aaacagcctg ataaataaaa cgccgtctga
     5041 acgccgacaa aatggttgac agcatcaggc ttgatgtata aactttcaaa tttttcgggt
     5101 cgttagctca gtcggtagag cagcggactt ttaatccgtt ggtcgagcgt tcgaatcgct
     5161 cacgacccac caggtaaagc aaaaaagccg agttttcaaa actcggcttt tttttgtttt
     5221 ccgacaatcc ttgttcggaa agcgcggcat ttcgtcaaat tctttggtgc ggcttccgta
     5281 tttttcccga aaatccctta taattccgcc tctgcaacct tcatcgggtg atgctttatg
     5341 cattgtcccg agtattgaaa tattagcaag tacccaccag gagggaacaa acgtggacaa
     5401 actgaaaatc tccgcaaacg gcccgctcaa cggggaaata acggtctcgg gcgcgaaaaa
     5461 cgcggcattg ccgctgatgt gcgcgggttt gctgacgtcg ggtacgttgc gcctgaaaaa
     5521 cgtccctatg ctggcagatg tcgcaaccac gcaaaagctg cttcagggca tgggcgcgcg
     5581 cgttctgacc gacaatatca gcgaatttga aatcaacggc ggtacggtca acaatacctg
     5641 cgccccttac gaattggttc ggacgatgcg cgcttcgatt ttggtgcttg gcccgacgct
     5701 ggcgcgtttc ggcgaggcgc aagtcagcct gccgggcggc tgcgccatcg gttcgcgccc
     5761 cgtcaatcag catttgaaag gcttggaagc gatgggggcg gaaatcgcca tcgaacacgg
     5821 ttacgtcaaa gccaaaggca aactcaaagg tgcgcgcgtg gcgatggatg tcgttaccgt
     5881 cggcggtacg gaaaacctgc tgatggcggc gacgctggcg gaaggtacga cggttttgga
     5941 aaactgcgcc atcgagcctg aagtggtcga tttggcggaa tgcctggtca aaatgggcgc
     6001 gaaaatcagc ggcatcggaa catcgaccat gattgtggaa ggtgcgggcg agctgtacgg
     6061 ctgcgaacac agcgtcgtcc ccgaccggat tgaagcgggg acgttcctgt gtgcggtggc
     6121 gataaccggc ggcagggtgg ttttgcggaa tgccgccccg aaaacgatgg aagtggtgtt
     6181 ggacaaactg gtcgaggcag gtgcggtgat tgaagcgggc gacgattgga tcgccatcga
     6241 tatgcggcag cgtccgaagg cggtggacat ccgcacggtc gtccatcccg gcttccccac
     6301 ggatatgcag gcgcagttta tggcattgaa tgctgtggcg gaaggaagct gccgcgtggt
     6361 ggaaacgatt tttgaaaacc gctttatgca cgttccggag ttgaaccgga tgggggcgaa
     6421 cattacaacc gagggcaata cggcatttgt gcagggtgtg gaacggcttt ccggcgcggt
     6481 cgtcaaggcg acggatttgc gcgcgtccgc cagcctcgtt atcgccggtt tggcggcgcg
     6541 cggcgaaacc gtggtcgaac ggatttacca cttggatcgc ggttatgaaa atattgaaaa
     6601 aaaactcggc agcgtcggtg cgaacatcga gcgcgtatcc ggctgatcgg gttgcgatgc
     6661 cgtctgaagg cttcaggcgg catcaattgt ttctgtatcg caaacactga ttgaaaagga
     6721 ctgattatgg catttttaaa actgaccgaa caaaacgtgc ggggcaaaac cgtcctcatc
     6781 cgcgccgata tgaacgtgcc gttcaaaggc ggcaaaatca gcgacgacac ccgtatccgc
     6841 gcctcgctcg cgtccgtcaa atactgcttg gacaacggcg cgtccgttat tgtgatgacc
     6901 cacttgggcc gcccgaccga aggcgagttc caccccgaag acgacgttgc gcccgttgcc
     6961 gcgcacttgg gcggcctgtt gggcaaagac gtgaaagtat tgaacgactg gcgcgaaaac
     7021 aaaccggctt tgaacgcggg cgatgtcgtg atgctgcaaa acgtgcgtat caacaaaggc
     7081 gagaagaaaa acgatttaga actgggcaaa gcctacgccg ccttgtgcga cgtgttcgtc
     7141 aatgatgcgt tcggcaccgc acaccgcgcc caagcttcga ccgaagccgt tgcccaagcc
     7201 gcgcccgtcg cctgcgccgg cgtattgatg gcgggcgaac tcgacgcttt gggcaaagcc
     7261 ttgaaacagc ccgcgcgccc gatggtggcg attgttgccg gcagcaaagt gtccaccaag
     7321 ctgaccatcc tcgaatcgtt ggcggacaaa gtcgatcagc ttatcgtcgg cggcggcatc
     7381 gccaacacat tcctgttggc ggaaggcaaa gccatcggta aatcgctggc ggaacatgat
     7441 ttggtggaag aatccaaaaa aattatggcg aaaatggcgg ccaaaggcgg ttctgtgccg
     7501 ctgccgaccg atgtcgtcgt tgccaaagcc tttgccgccg atgccgaagc ggtggtaaaa
     7561 gacatcgccg acgttgccga agacgagatg attttggaca tcggcccgaa atccgccgcc
     7621 gcgcttgccg agttgcttaa agccgccggt acggtcgttt ggaacgggcc ggtcggcgtg
     7681 ttcgagttcg atcaattcgc aggcggcaca aaagcccttg ccgaagccat cgcccaaagc
     7741 aaagcgttct cgattgcggg cggcggcgac acgctggcgg cgattgccaa attcggcgtt
     7801 accgaacaaa tcggctacat ctccaccggc ggcggcgcgt tccttgagtt cttggaaggc
     7861 aaagagctgc ctgccgtagc cgctttggaa aaaacgcggc gagtaaacgg tttgatataa
     7921 aaaaatgccg tctgaaacga aatggtttca gacggcattt ttgtgtggcg gttattgtgc
     7981 tttggcagcc cattccgccg gagtggcggc aaccgaaatg gaaagtgcgt gcagttcgtt
     8041 gctttccagt tgggctttga gtccgtcttt aatcaggcgg tggcgcgcga ggcgtgcctt
     8101 gccttcaaat tctgatgaaa cgatgacggc gaaaaaatgg tgtccgtcgc cttctacttc
     8161 gatgtgttcg cattttgcca cgcctgcaat cagggccttg acttgttcgg gtgtcagcat
     8221 agtggggagt ccttattggg gaaatgcgcc attatactgg caaatcgaaa ttccgtctga
     8281 aaacggcttc agacggaatt tttgacggtg ttttattttt tggctttgaa gcagagcagg
     8341 tgttgtgtgg tggcaagcca cgcgccgaat acgcccaacg cgatgacgaa gccgaacacc
     8401 agcccgagtt cgccgacgta gaagaaacgc cagccgatgt tcaagccgta gggtttgaag
     8461 atggcatcga ccaaggggtg cacggcagag agcagccaac cgcaaagccc taagctgacg
     8521 gcggcggaga agatactctg ccacatagcc tgataaagga aggggcggcg gataaacgac
     8581 gcgggcgcac ccaggagttt ggtgatttcg atttcttctt tgcggctgag gatttgcagt
     8641 cggatggtgt tgtgtgcgac aaggacgaaa gccataccca gcgtcaggga aagaaaccat
     8701 aagattttgc ggatgaactc gttgatttgg tacagcgttt gcacccattc ggtatccata
     8761 gacgcggatt cgaccatagg cagcttggta atgtcccggt agattgcctg catttgggcg
     8821 ggcgtggttg ccgggtcggg ggtaacgata aagacatccg gcagggggtt gccgtcaagc
     8881 atggaaatca gattttggtc gaggttggac tgtaattccg ccaaaccgtc ttccttgccg
     8941 atgaagcgga tgttgtcgag ccgtttatcg cgcgtcagca ggctgcgtac ggtatcgctg
     9001 tcgctttgtg cggcggcggt ttccatatag acggtgattt gcggcgactc gttgagtttg
     9061 cccaacacgc tttgcccgct ttggatgccc agatacataa acagcggcag ggtcatcgcg
     9121 acggcaagca ttatgagggt aagcagtgtg ccgaagggtt ggcgcagaag ctgtttgagt
     9181 gcggagcgcg cggattcgac gtgcagcgag aagtagtgga tgatgctcat gcgagtcgtc
     9241 ctttcgagag gcgcaggatg cggtgtccgt agtccgccat cagggtttcg tcatgtgcgg
     9301 cgacgatgac ggtggttccc gcttcgtgga aggttttgaa caattccata atatcgagcg
     9361 cgtaggcacg gtcgaggttg gcggagggtt cgtcggcaat cagcaggccg ggctggtgga
     9421 cgacggcgcg ggcgatgcac aggcgttgtt gttcaccgcc cgagagggtt acgggatcgt
     9481 ccaattctcg tcctttcaag ccgacttttt cgatggcgat gcgggcgcgt tcttcggctt
     9541 tgcgcggcgg atagccgata atccgcaggg gcaggatgac gttttgcagg acattgcggt
     9601 cgttgaggat tttgtggtct tggaacacga tgccgatgtg ttggcgcata aagccgattt
     9661 ggttgtcgga caatgtgccg aggtcctgcc cgttaaacag gactttgccc atgctcggct
     9721 tggtgatgcc cgaaatcagc ttgaggacgg tggatttgcc cgaaccggag tgtcccgcga
     9781 taaaaatcat ctcgcctttg ttgatttgga agctgacgtt tttcagggct tcaaaaccgc
     9841 cgggataggt tttggaaact tgttcgaaac ggatcatagg aggtcctgta aggaagggcg
     9901 gcatatcgtt tcgggcggtt gcgtccgcag ggatatgccg tctgaacaat aggtaagaca
     9961 gtaccgatta taaagattcc gcctgttttt agatagtgga taagtttggc ggataccgaa
    10021 ggccgggcgg gaaaaatgcc gtctgaagcg gcttcagacg gcatccggcg tttaacggca
    10081 ttttgaatgg gcgagtttga cggcttcggt caggcttttc tcgttcacct ctcctgtaat
    10141 ggtctgcctg tatccgcatt tcggggcttc gacgacggta aagggcagta cgccgacatt
    10201 gtttccgtag gatttcataa agtttcggct gttcgccccg gtgtaacgcc aaatcgggta
    10261 gctgaccggg gtctgcttga gaaagttgcc gatattgtcg gatgtgtcga gcgcgatgcc
    10321 gaccatatcg acgctgcctt ttttctgcgc tttgtaccat ttggacatgg cgggcatctc
    10381 ttttcgacac ggaccgcacc aagtcgccca taggttggcg atgcggacgg gggctttgag
    10441 cgattgcagg ttttgcgggg tattgtcttt ccatccggcc agttcgtctg ccgaggcggt
    10501 atgtgcggca aatgtggcgg caagggcgat tgcggcgaga ttcaagcgtt tcatcgtttt
    10561 gtcctttgta tgtcgattcg ggtattgtaa accgtttggt acggttggag gcggggtatt
    10621 gagtaaaacc ctgcgtaagc cgataataag gggattgtcg agaagtaggg aaatgtcatg
    10681 tctgaaatca aaattttcca caatccgcgt tgcagcaaat cgcgtgccgc cgtgtcccta
    10741 ttggaagaac gcggcattgc tgccgaagcg gtcaaatatt tggatacgcc gcccgacttg
    10801 tccgaattga aggatatttt caacaaattg ggcttggaat cggcgcgcgg gatgatgcgc
    10861 gtgaaagatg atttgtacaa ggaattgggt ttggacaacc ccgatttgga caatgacgcg
    10921 ctgctgcgtg ccatcgccga tcatcccgcc ctgttggagc gtccgattgt tttggcaaac
    10981 ggtaaggcgg ctgtcggcag accgttggaa aatatcgaag cggtattgtg aacaaacggc
    11041 tgttttgcag ccgcaacggc ttgcgctatt acctgttggg cggtttctgt ttgagtgttt
    11101 ttccgctttt gctggtgttt gcctcatccg tttgggcggt ttatcggacg ggcgggcagg
    11161 ttttgccgcc gtatgtccgg gcggatgccg cgctggtgtt gggcgcagcc gcgtgggaca
    11221 aacgcccttc cccggttttt cgggaacgca tcaaccacgc catcgcgctt taccaaagcc
    11281 gcagggtagg caaaatcgtg tttacgggag ggcgaaccaa aaaaggctat atgaccgaag
    11341 ccgaggtcgg gcggcggtac gcgctcaaac agggggttcc ggcgcgcaac atcctgtttg
    11401 aaaacacttc gcgcaacact tatgaaaacc tgaacaacat ccgtccggtt ttgcgtgcca
    11461 acggtattgc cagcgtggtg attgtcagcg atccctacca tttggcgcgg gcggcggaaa
    11521 tggcggagga tttgggtgtc cgcgtttata tgtctgccac gccgaccacg cggtttgatg
    11581 cgggaaataa gaaaaaaatc tttatgctgc aagaggggta tgcgttatcc ctctaccgtt
    11641 tggaaaaatg gggcagccgc tttttgggat ggctgtctga ttgaagcggt agaaaatgcc
    11701 gtctgaaact tatccgcagg cagggttcgg acggcatttt tgatgcctaa gcacggtaaa
    11761 aacagtaaaa taaacgcttt caaagatttc ggagccatta tgaccgtcaa aacccgtttc
    11821 gcccccagcc ccaccggtta cctgcacatc ggcggcgttc gcaccgcctt gttttcgtgg
    11881 gcgtttgccc gccatcataa aggcgaattt ttattgcgta tcgaagacac cgacttggca
    11941 cgttccaccg ccgaatccgt caacatcatc cttgacggca tgaaatgggt cggtctcgat
    12001 tacgacaacg ccgacaacgt ggtgtaccaa acccgccgtt tcgaccgtta taaagaagtc
    12061 attgccgaac tcttggcaaa aggcgatgcc tactactgct attgcagcaa agaagaattg
    12121 gaagcgatgc gcgagaaagc cgaaaaagaa ggcacggcga cttacgaccg ccgctggcgg
    12181 ccggaagcag gcaaaaccct gcccgaaatt cctgcaggcg tgcaacccgt cgtccgcttc
    12241 aaaacgcctt tggacggcgt taccaaatgg acagacttgg tcaaaggcga aatctccatc
    12301 cccaacgaag ccctagacga cctgattatt gcccgcgccg acggtacgcc gacctacaac
    12361 ttctgcgccg tggtagacga ctacgacatg ggcgttaccc acattatccg cggggacgac
    12421 catgtgaaca acaccccgaa acaaatcaac atcttgaaag ccatcggcgc gaacctgccc
    12481 gaatacgggc atctgccgat gattctcaac gaacaaggca aaaaaatctc caaacgcagc
    12541 ggcgataccg tcgccattac cgatttcggc gcaatgggca ttctgcccga agcgatgctc
    12601 aactatttgg cgcgtttggg ctgggcgcac ggcgacgatg agtttttcac gacggaacaa
    12661 ttcatcgaat ggtttgattt gaaagacgtt tccccgtccc caagccgtat ggatttgaaa
    12721 aagctctatt ggatcaacgg cgaacacatc aaaatcacac ccgacggcaa acttaccgaa
    12781 ctcgtcaaac cgcgccttgc gctgcgcgat attcatgaaa ccgaaaaacc tgcgttggaa
    12841 gacgtgttgg cactggtcaa agaccgcgcc caagatttga acgccttggc agacgaatgc
    12901 ctgtatttct acaaaaaaca agtccctgcc gaagccgatg ttgccaaaca ctgggacgac
    12961 gaagccgccg cccgtatgct gcgcttcgcc gaacgcctcg aagggctgga agactggaac
    13021 gccaaagcca ttcacgacct cttcaaacct ttctgcgacg aagaaggcat caaaatgggc
    13081 aaactcggta tgcccctgcg ccttgctgtc tgcggtacgg caaaaacccc gagcgtcgat
    13141 gccgtgttgg cattaatcag caaagaggaa gtgttgaaac gcatccgtgc ttaagttatc
    13201 cacaggtgcg ggcaatgccg tctgatgtcg gaatgcggct tcagacggca ttcagacgga
    13261 agttgaacat gaaaaaaata ttgtattttc tgatgtttgt tttttctaca agcgtatggg
    13321 cagggggtgc ggaagacaat ttgctcagca tccaatccgg ttaccgtgct ttactgcaaa
    13381 agcaaaacaa tctggacgga aaaatcatcg ggatgcagtc ggatttggaa gatgcgcgcc
    13441 ggcgtttgca ggcggctcag gcagacatcg cccgtttgga agcggaaatt cctgcagcaa
    13501 tggcccaaaa agcccggcag gctgaagaga tgaggcaaat cggagtgcgt ttggaccatg
    13561 cttggaatgc ggtttacggc gcaggggaac gaaagcgtcg ggggaattga ccgatgaatt
    13621 ccctctttgt ggacaacact gtttttatta cacggctgaa agccgggcat atcggcaggt
    13681 tggttcaggc gttgtttgag gagtggcacg gatttgaacc gtggtcttct gtggataaga
    13741 ttcgtgccta ttacggcaga tgtttgaagg atgacgaatt gccgctggct tttgcggctg
    13801 tggatgattc cggaaccctg ttgggttcgg ctgcagtcaa gcggcatgat atggaaaatt
    13861 ttccacagta tgaatattgg ttgggcgatg tctttgtttt acctgaatat cgcggaaaag
    13921 gcattggcag gaggctggtc gcccactgca taggcgcagc gcgttcgctg gggataaagt
    13981 tcttgtatct ttatacgcct gatgtgcaaa tattttatga atcattcggc tgggcggttg
    14041 tcgggcgaca tttccataac ggggaatggg ttacggttat gcgtttggat gtggataagg
    14101 tttaagccga atgggcagat gccgtctgag ctgttcggac ggcatttttt atgtttggaa
    14161 atttcctgtc agatatagtg gattaacaaa aaccggtacg gcgttgcccc gccccggctc
    14221 aaagggaacg attccctaag gtgcccaagc accgggcgga tcggttccgt accatttgta
    14281 ctgcctgcgg cccgccgcct tgtcctgatt tttgttaatc cgccataaag tttatccaca
    14341 gatttttcca tatggtttga tgctgctccg ccaacggaaa ttaagatacg gatttgcaca
    14401 aatctttcag acggtacagt tctgacaatg cttcgcgcgg ggtcaggctg tcgggatcga
    14461 ggttttccag ggctgctgcc aatatacctt caaaatgttt ttcctctgct ttatccacaa
    14521 agcagtccac attcggttca tctccttttt cagacggcat ggtactgaaa atatccagtt
    14581 ggggacggtt tgcggcggct tggttttcca gtccgttcaa atgcttttgg gcggctttca
    14641 atgcgcgcac aggcaggccg gcgagtttgg cgacggcgat gccgtagctt tttccggcgg
    14701 gaccgggttg gatttggtgc aggaacacga tgtcccgtcc ctgttcgagc gcggaaaggt
    14761 gcatattgac ggcggcggcg tgggcttcgg gcaggtaggt cagctcgaaa tagtgggtgg
    14821 caaacaggct gaaggatttg tttttttgca gcaggtgttc ggcgattgcg tgcgcgaggg
    14881 cgaggccgtc gaaagtggaa gtaccacgtc cgacttcgtc cattaaaaca atgctttgtt
    14941 cggtggcgtg atgcaggatg taggcggttt cgctcatttc gaccatgaag gtggagcggt
    15001 tggaggcgag gtcgtccgat gcgccgatgc gggtgaagat ttgatcgaca ggcccgattg
    15061 tggcggcatc ggcaggcacg aaacagccgg tgtgtgccaa taaaacaatc agcgcgactt
    15121 ggcgcatata ggtggatttg ccgcccatat tggggccggt gagcagcatg aggcggtgtt
    15181 tgtggtcgag gtcggtgtgg ttggctgtga agtggcgtac ctgctgttcg acaacgggat
    15241 ggcggccgtt ttcgatgtgg acaaccggat agtcggcaaa ctcgggacgg acaaagttcc
    15301 gctcttttgc caaggctgaa aatgtggaca acacgtccag cgcggcggcg gctttggcgg
    15361 ctttttgaag ctgcggcaat gccgtccgaa ggtttttcaa tacgccgtca aagagttgtt
    15421 tttctaaggc gagggcttgg tcttgagcag tcagcacttt gtcttcaaag gctttcagtt
    15481 ccggcgtgat gaagcgttcg gcgtttttga gggtttgccg gcgttggtag tcggcaggtg
    15541 cttgttcggc ttgggttttg gacaattcaa tgtaaaagcc gtgaacgcgg ttgaactcga
    15601 ctttgagtgt ggacaaaccg gtacgttcgc gttccttggc ttccaaatcc agcaaaaatt
    15661 cgtcgccatg gttttgaatg cggcgcaatt cgtccagttc gggatgaaaa ccgtggttga
    15721 tgacattgcc gtctttcagc cagacggaag gttcgggcat cacggcggct ttgagggttt
    15781 cggcaactgg cagggtttcg gggaaaacgg ctttaagggt ttccaacagg ctgctgccgg
    15841 ttgcggacaa gtcgatttgt gccaactcga acagactgtc gcgtaaagat gccaaatcgc
    15901 gcgggcgggc attgccgacg gcgatgcggg cggcgatacg ttcgatgtcg gcaatgctct
    15961 tcagatggca ttggaggggt tcgtattggc tttccagcgc ggttacggct tcttggcgcg
    16021 ctcggatgtg ggcgcggttg cgtaaagggt ggtgcagcca caatgccaac aggcggctgc
    16081 ccatgtgggt ggcgcagccg tcgagtatgg aaaacagggt cggcgttttt ttgccggaga
    16141 gggtttgcgt gatttcgaga ttgcggcgcg tggcggcatc catgccgata tattggctgt
    16201 cggtttcgag cgacaggccg tccaaatgtt gcggcatcag gttttgcgtc aggcggatat
    16261 agttcaacag cgcacctgcc gcgccaatcg aaacggcgtg ttctttgctg tccaaaccga
    16321 agccgcgcaa atcttggcag ccgaaatatt ccgtcagcag tttttccccc gcgtcggcgg
    16381 caaactgcca cgcgttcagg cgtgtaacac ccgatgccgt ctgaagttgc ggtgcgtttt
    16441 tactgtccgg cagcaaaatt tccgccgcct gcaggcgcgc cagttcgtcg ttcaatttat
    16501 ccgcagttgt cagcttggtt ttgaattcgc cgctttgcag cgatgcccac gccaaaccga
    16561 tgtatttttt gtcgggagac acggcaacga tgcggttggt ttccttgtct tccagtaatg
    16621 cggaatcggt tagcgtgccg ggcgttacga tgcgcacgac tttgcgctcc accggccctt
    16681 tgcccgcgcc gacttcgccg acctgttcgc aaatcgccac gcttttgccc aacttgacca
    16741 ggcgcgccag atactgttcc gccgcgtgaa acggaacgcc cgccatttta atcggcacgc
    16801 cgtccatctg cccgcgtgtg gtcagggtga tgtccaacaa tttcgccgct tccaccgcgt
    16861 catccaaaaa cagctcgtaa aaatcaccca tacggtaaaa cactagtttg tcggtatgtt
    16921 gcgcttttat gccgaggtat tgctgcatca ttggggaaac ggcggatttg gacatggggc
    16981 tgccttatta ttaggaaaac ggtattgtag caaaaggttt tgtcttcgac ggaatgagac
    17041 tgaccgtaag cttgtcaata aagttgtgga gacaaatagg aaaacggcaa agttatccat
    17101 aagaatatta ttaagtcaaa aaacatccac gcttatctgt cttatacggc aagccttaaa
    17161 ctcaaattta tttaacaaaa aggtaatgaa aatattggat atttgctact tatacacaag
    17221 caggtcggca atcatcaaaa tcatcattaa aattttaaaa tcaaatattg aatgcgcgtt
    17281 tttgaaatag cgaaattcac ctgcgtcaaa caaaacgcaa gtaattttat gtaatagtgg
    17341 aatgtagttt tatttcagaa tacaatacag gcatcggact ttatttcaca accgggagac
    17401 aaaatatgag catcaaagta gcgattaacg gtttcggccg catcggccgc ctcgcattgc
    17461 gtcaaattga aaaagcccac ggcatcgaag tcgccgccgt caacgacctg acccctgccg
    17521 aaatgctgct gcaccttttc aaatacgaca gcacacaagg ccgtttccaa ggtactgccg
    17581 aattaaaaga cgatgccatc gtggtaaacg gcagagaaat caaagttttc gccaatccga
    17641 atcccgaaga attgccttgg ggcgagttgg gtgtggatgt cgtcctcgaa tgtaccggtt
    17701 tctttaccaa caaaaccaaa gccgaagcac atatccgtgc cggcgcgcgc aaagtcgtga
    17761 tttccgcacc cggcggcaat gatgtgaaaa ccgtcgtata tggcgtaaac caagacatct
    17821 tggacggtag cgaaactgtc atttccgccg cttcctgtac caccaactgc cttgccccga
    17881 tggcggcagt cctgcaaaaa gagttcggcg tagtcgaagg cctgatgacc accatccacg
    17941 cctacaccgg cgaccaaaac acccttgacg cgccgcaccg caaaggcgat ttgcgccgcg
    18001 cccgcgccgc cgcgctcaac atcgtgccca acagcaccgg cgccgccaaa gccatcggtc
    18061 tggttatccc cgaattgaac ggcaaactcg acggctccgc ccaacgcgtc cctgttgcca
    18121 ccggctcgct gaccgaattg gtttccgtcc tcgaacgccc tgcaaccaaa gaagaaatca
    18181 acgccgcgat gaaagccgcc tccagcgaat cttacggcta caacgaagat caaatcgttt
    18241 cttccgacgt tgtcggcatc gaatacggct cgctctttga cgcaacccaa acccgcgtga
    18301 tgaccgtggg cggcaaacaa ctggtgaaaa ccgttgcctg gtacgacaac gaaatgtcct
    18361 acacctgcca actcgtccgt actttggaat acttcgcagg caaaatctaa ggcttgaaca
    18421 aacctgtgga taagtaaatg ccgtctgaac acattgcgtt cagacggcat tgtttatctg
    18481 taacttgata ttgcggctta tggcgaacaa acaacagccg tttgttcaac tgctatgcgg
    18541 caaaaccgga ttttgcccga tttaaagcgg ggttggggaa agattaataa ccgtcgcgcg
    18601 gttttcctcg atttgaaacc tgacttcgta atccgcaata ttcatcacat aaatccgttc
    18661 gggaatattc tgataggcgg ggcgcggatc ttgggcaatg ctttggctga taaggttttt
    18721 ggtgtttgca gataaatttt ccgcgccgat gttttcctgc caaacgactt ccaactctac
    18781 gggtttgccg ctgacgaaac cggatgctgc atcgggtttg gattcgataa aggggatata
    18841 aggcttgatg tccacaatcg gcgtgccgtc cagcaggtct gagccgctgc aatagaggcg
    18901 gacgggtttg ccggtttcga tgcgttcgag tttaaggaga gagagtccga gatggttggg
    18961 gcggtggggg ctgcgcgtgg cgaacacgcc cattttttgt ttgccgccga gccgtggcgg
    19021 gcgcaccatt tgcgcccaac cttcatccaa tacgccgtgg aagataaaac ttatccacac
    19081 ataatcgaaa tcttccaacc cgcgcacgct gtctgcggta aattcgggat tcagctcgat
    19141 gcaggcttct gcggcggaga ccaaaccggg ctggcgggcg atgccgaatt tctgtttgta
    19201 gggcgagcgg gcggcgccga tgggggtaat ggtataagtc ataagattgt ggataagttt
    19261 tttggaaaaa ccttatctta tcacagccgg ttttaccgct ttcgggtagt aatttgttat
    19321 aatacagggc atttaaatcg cgttgaaata aagtagcaca gaatcctcgt gtccaagacg
    19381 ctcttaaaaa ggcattggag tattgataaa aaagaagcat aacggttcgt tataacgaag
    19441 agggaatccc gtatattaca cccaagtcag gttgttatct ttcctttcag atctcaccta
    19501 caggaggtga gatctgaaat tttgggtaat aaaatcggat tacagctttt agcaaggagc
    19561 accttaggta tagctaaaat ggaggaggtg gatatgacat atcatatcca cctagatgga
    19621 ttgtgctcct taaataaaga aggtaagagt tttgttattc gtaaaataga gaatgacgat
    19681 cttaagtgaa gaagtaatga gatgattgtt tccattgatg ttgatgcaca aaaaacgttt
    19741 acgccactgt gtcctgacga actgcccgtg aacgaggggc atttgattgt cgaggagtta
    19801 aatgcccaag ccgctttggc ggatttgcgt gtgatgacga aagatgcgca tcatatggcg
    19861 gcgaaatggc ttgtggataa ccctgttgat atgttgaagc cgacaggttt ttccgatgcg
    19921 gatttgactt gggtggctca tgcgatggtc ggtacgcgcg gctatgaatt gttggacggt
    19981 ctgccttccg tcaaggaata cgattattgc gtttggaaag gtgtcgatcc cgaattgcat
    20041 ccttacggcg cgtgttttca cgatattgag gaaaaactga gcactggatt gattgagtgg
    20101 ctgcgttgtc aaaatacgaa tatggtcatt gtcggcggct tggctacgga ttattgtgtt
    20161 aaaacaacgg ttttgcagtt acttaaaggc ggtcgttggc aggttatcgt gaatgaagcg
    20221 gcttgtcggg gtattgcgcc cgataccatt gaagcagcgt ggcaggaaat gcgttcttcg
    20281 ggtgcgataa ttttgaaaaa cgccgaaaaa attaaaaaat atattaataa tcaataactt
    20341 ataattgttt cacgtgaaac accctgtcta catatgaaaa ttttgcttgt ccgtttgtcc
    20401 agtatgggtg atttaattca cactttgccc gcaatcgaag atttggcgcg acaatgctct
    20461 gatgtagaac tgcattggct atgtgaagcc gggtttgcgg gtattgcacg gttgcatccg
    20521 tttgtaaaaa aggtccatgt aatgaagtgg cggcaatggc gcaaacatct ctttcaggct
    20581 gaaacttggc gggagatggg tcgtctgaaa caggctttgc tgcaggaagc atttgatttt
    20641 gtattggaca gtcagggtct gattaaaagc gcgtgtttcg ccaaaatggc aaaatcccct
    20701 atttatggtt tggataaaca cagtgcgcgt gagggggtgg ctgctttggc gtatgataaa
    20761 aagtatgtcg taccgaaagg tagaaatgcc gtttggcgca accgtgatct gtttgcccag
    20821 gtgtttgggt atgcaatgcc tgagacgcag gtatttggtt tgtccgttcc tgaagcaagc
    20881 cgtctgaaaa atttagcgca gccgtattat gcggctttgc acgcgaccag tcgggacagt
    20941 aagttatggc ctatggaaaa ttggcgggag ttgctgcaga aactgaatca aaaacaacaa
    21001 tgcaatgttt atctgccttg gggaaacgag gctgaaaaag tgcgtgcgga acagattgca
    21061 gacgggctgc cgtttaccat agtgtgcgct aaaatgaatt tattacaggc ggcgtatctg
    21121 ttgaaacacg cggttggaat agttggtgta gataccggtt tgctgcattt ggcaaatgcc
    21181 ttggaaaaac ctgtggtcgg catttatacc gataccgatc cgattaaaac aggcgttcaa
    21241 gtttcggcta ttgcaaaaaa tgtgggcaat atcgggcaga ttccgaccgc agatttggtt
    21301 tatcaaacgc tgatggattg tgtttcagca gataaaggct aaagggtcgt ctaaaactga
    21361 tatggcggca tgatattttc caatttatta gatacaggaa tgtatatccg ccagactgag
    21421 taaatacttt aaaaatggta taatcggtaa aattatccga aagattgcta aataaattcg
    21481 tttttaagga aaacggattt gattttataa agaagaaggt ttggtttcgc tggatggtga
    21541 aagcagcgtt ctttttagga gaattcaatg aaagcactgg tcgcagtaaa gcgcgtagtg
    21601 gactacaacg tcaaagtccg tgtgaaagcc gatggttcgg atgtggacat cggcaatgtc
    21661 aaaatgtcga tgaacccgtt tgatgaaatc gctgtggaag aagccgtcca tttgaaagaa
    21721 gccgggaaag taagcgaaat tgtagcggtt tctttgggtg aaaagaaatg cgaagaaacc
    21781 ttgcgtaccg ctttggcaat gggtgccgac cgtgccattc atattgaaac cgatgtgaaa
    21841 ttagagccgc tggcagttgc caaactgctg aaagctgttg cggacaaaga aaatccgcaa
    21901 attttctttt tgggcaaaca agcgattgat gacgatgcca atcaagtggc gcaaatgctg
    21961 gcagctttgt tgaatgcggc gcaaggtaca tttgtttcaa aagtacaaat tgaaggtgat
    22021 gaagtacaga ttgtgcgcga aattgatggc ggcgaagaaa cgattgcgtt gaaactgcct
    22081 gcagtcatca gcgcagattt gcgtttgaac gagccgcgct ttgtcaaact ccccaatatt
    22141 atggcggcaa agaaaaaacc tctggaaaaa ctggattctg ccgatttggc taccgatatt
    22201 tcgcctcgtc tgaaaacggt gaaatttgcc gaacctaagg cgcgtcagac aggtgtaaaa
    22261 gtagcaagcg ttgccgaatt ggttgaaaaa ttgaaaaacg aagccaaagt gatttgagga
    22321 ggctgaaatg agcgtattga ttattgccga acacgacaac aaacagttga atcctgccac
    22381 tttgcatgct gttgccgctg ccgccaaact gggcaaggtc gatttattgg ttgtcggaaa
    22441 cggcgcatcg tccgtcgtgg aattcgcgaa gcaagtagcg ggagtggaaa aagttttggt
    22501 tgcagatgct gctcattatg ctgaaggttt ggctgaagag ttggctccgc tggttgtcaa
    22561 attggcggcg gattaccgct atgttgcagc aacggcaacc acattcggta aaaacctttt
    22621 gccccgcgta gccgccttat tagacgtacc gcaaatttct gatttgaccg agattgtaga
    22681 taacacgact tttgtacgtc cgatttatgc aggtaatgca tttgaaaccg tgcaagccga
    22741 ttcagaaaaa ttggtgctga ccttccgtgt gacggctttt gacgcagtgg cggcgcaagg
    22801 tggtaatgct gaggtaatta atgttaaaac aacccctgcc caaaacctga gtcgttttgt
    22861 taaccgtcaa ctttcccatt ccgatcgtcc cgaactgact caggcaaaag tgattgtttc
    22921 cggtggtcgt gcgttgggta gtgtggaaaa attcagtgaa gtgctgacac cgttggcgga
    22981 tgttttaggt gcggcaatcg gtgcatcccg tgcggcggtt gatgccgagt atgcaccaaa
    23041 cgatgctcaa gtcggacaaa ccggtaaggt ggttgcgccg cagctctatt ttgcaatcgg
    23101 tatttcaggt gcgattcaac acgttgccgg tatgcaggac agtaaggtga ttgttgcaat
    23161 caacaaagat gccgatgcgc cgattttcaa tgtagccgat tacggattgg ttggcgattt
    23221 atttgaaatt gttccacaat taatagaatt attgaaaaat tgatgtttca cgtgaaactt
    23281 tcaggcgact ttgttattaa tgaggtcgtc tgaaaacatt tcagcatgat tacgacttat
    23341 aaaaccatta cttccccgac gcaggctgag tttaaagata aaggcagccg ttttattgca
    23401 tttgcctatc cgattcggac attggctgat gtgaaaaaat acctcaatct gttaaaggaa
    23461 gagcattata aagcgcggca ttggtgctat gcctatcgtt taggtgtgaa cgggatgcag
    23521 tttcgcggca acgatgatgg agagccacca ggaagtgctg ggcggccaat tttgggacag
    23581 attgattcgg tgggtatcac tgatgttttg gtcgtggtcg tccgctattt cggtggtgct
    23641 ttattgggcg ttcccggctt gattcacgca tacaaagagg caacggctca ggcgttggcg
    23701 atcgcggaag tggttgaaaa gaatattgaa aaaaccgttt ggctgaaatg tgagtatccg
    23761 tttttgaatg aagcaatatg cattgccaaa caatatcagg cggatatatt ggagcaggat
    23821 ttgcagttgg attgccgatt gacggtaagt ttgtcgttgg taaattatga ggcctgtgtt
    23881 gcggcttgga agaatacacg gcaggttgaa gtaaatacgg aaaagccttt tgaatgaaag
    23941 gttgtatgaa atgctttgta atggtatttc aggcaacctc ttcgctgatt aattttcttg
    24001 tgtatatctg tctaaggtat cgacgctgga gcataaatat agccaagcca attgatggaa
    24061 ttcgcccggc gcgttccata atgcatcttc actcataatg ctatattcgc cttctgtttg
    24121 caaattcagt tctaccccgt cttcaatagt ctgatggcgc acctgatact cgtgggtata
    24181 gaaattgtcc atatcgcgaa ttaaagcgac ggcatatttc caacggcgga tgttttcttc
    24241 cagttgcagt agtaaatgac accattcgcg gtatttgctt tggatttcat caatacgttt
    24301 ttgcgtgttt gcttgttctt ttttaatttg tgaataagtt gaataggttg cagagtggaa
    24361 tgaagtggtc ttttgtttga ttctactaaa atttcttcag atgggacaaa agttcctttg
    24421 tatcgagttt tcgttaaaag tacatatttg tcaaggagat ggcatgaaac tgctggttat
    24481 cggtaatggc ggtcgcgaac acgcgctggc ttggaagttg gcgcagtcgc ccaaagtgga
    24541 aacagtattt gttgcgcccg gtaatgccgg tacggcgatt gaatccaagc tccaaaacat
    24601 tgccttgacc gcgtatcagg atttgattga attctgccgt aaagaaaata ttgtttttac
    24661 cgtcgtcggc cctgaagcgc ctttggcggc aggtattgtg gatgattttc gtgccgcagg
    24721 gctgaaaata tttggtccga cacaatatgc ggcgcagttg gaaagttcta aagatttcgc
    24781 caaagcattt atggtgaaat acaatattcc gaccgcgcaa taccaaacct ttgaaaatgc
    24841 cgatgccgca catgattacg tcaatcagaa aggtgcgcct atcgttatca aagccgatgg
    24901 tttggtggca ggtaaaggcg tgattgtggc aatgactttg gatgaagcgc atgctgcgat
    24961 tgacgatatg ttgttgggca ataaaatggg caatgccggc gaacgcgttg tgattgaaga
    25021 ttttctgcaa ggcgaagaag cgagctttat cgttatggtt gatggcaatc atgtattacc
    25081 tatggcgacc agtcgagacc acaaacgtct tttagacgga gataaaggcc cgaatacggg
    25141 cggtatgggt gcgtacagtc ccgcgcctgt ggtaacgcct gctgtgtacg agcgtgcgat
    25201 gaacgagatt attttgccga ccgtagcggg tatgaaagcg gaggggcacg agttcaccgg
    25261 ctttttgtac gcaggtttga tgatcgatca aagcggtgca ccctatacga ttgagtttaa
    25321 ctgccgtttc ggcgatcccg aaacccagcc gattatgagc cgtctaaaca gcgatttggc
    25381 ggatttggtt gaagcggcaa tagacggcag actggacagc gtcaaagcgg aatggaatcc
    25441 gcaaactgcc gtgggtgtgg tactggcggc gcaaaattac cccgaaacac ccaagaaagg
    25501 cgatgttatt tccggtttgg atgacgtcaa ccgaatcggc aaagtattcc atgccggcac
    25561 aacggtaaac gagaaaggcg acgtgttgac caacggcggg cgcatattgt gtgttgtagg
    25621 attgggcgac gatgtggcgc aagcgaaagc caaggcgtat ggcgcgttgg aaaaaatcag
    25681 cttcgacggg atgcaatacc gtaaagacat tgccgataaa gcgattaacc gttaattaga
    25741 aagaggatga tgccgtctga agccaagtat gactgatgcg cttcagacgg cattttatat
    25801 tatgttattt tcgagaatta tcgcagcatc agcgcaacgt aagttaagcg tttatttgaa
    25861 aaaaggcggt ttggttgctt atccgaccga atcttgttat ggcttggggt gtcttccgac
    25921 attagccaaa gcactcggta agttggcgca tttgaaaaaa aggccgcaac acaagggtat
    25981 gattgtcatc gggaatcaac ttgaacaatt gcagccttta cttcaaatgc cgtttgaaaa
    26041 cattcaaacc atgcttagaa acgaatggcc tgctcctaag actttcctat tgttggctaa
    26101 atctggcgta ttacctgcgt tgcgcgggaa gaggcgcagc aagcttgcgg tcagagtgcc
    26161 cgatcataca ggtgcaaggc gtttgtgtca ggcattgggt atgcctttgg tttccacttc
    26221 atgtaatcgg gcgggtaaac gcgcgtgtcg gacggagagg gaagtcagaa ggcagttcgg
    26281 acgcgatgtg tggattgtcg gtgggagaat cggcaggcag aaatcgccga gtcagattat
    26341 cgatggggaa acaggtaaac gtttgcgtta gatatggatg cttgagtgaa aacttttttg
    26401 catacacaaa gaaaaaattt tttggatggt atttctctac atgattgtta tcgcacggcg
    26461 ataaaggtag aaggacgcaa cgtctgcttt gattttgagg acggttttgt cgttctggac
    26521 aataatccga acaatcaagc gggcaagcat ctgaaaacag atttttcccg tgttgtttta
    26581 acccatgaaa atagaaacgc cgaagacgac tatttggtcg atatttttga agacatcgtc
    26641 tttttaggga aacgtttgtt tacagtcaga aaatttcttg atttttctga attgcttgaa
    26701 atgatcaatg cacaaggcta ttttcttgaa tttctgtatc tgtatgaatg tatcggtgtc
    26761 aaaacaagcg attatttttt agaggctgtg ttggttacgg gacggagccg ggaatgtaaa
    26821 aaatgcttta tcaaaattat gagggcaagc agttttgtgt atcaatggaa caatttacgt
    26881 ttgaatagtg agattgtata gcgggtttat atttcggatg gaagccggtt taaaaaaata
    26941 atgtggataa tttcagggaa tggcaaaatg tcgtctgaaa tgctgatatt tcagtattcc
    27001 agacggcatt tttataataa ggcttatgga taaatttgtg tataagttaa aatcagagtt
    27061 ttttcccgca tacggtttca tattgttttt cagttaatgt cccggacagg atgctcatcg
    27121 ggcggaggga agaggctgtg tagttttgtg tggaaatttc cgtgtttttt gtatcaaata
    27181 actgtagcga acttaagcgg tatgttttgt tgttgcagtg gatttcccac tcggcaatgg
    27241 cagtcttgta tgcgggggtg ttggcaaaac gttcttgttt cagattggta acaacttttt
    27301 tatcttggaa aatcatcaga tttccgtttt ttctcacact gtctttatta atatatgtat
    27361 gaatattgcc gtttgagatt gtgccgatat tttcccatcg ggcagacttg tccggtgtgc
    27421 tgcaggcggt tatcaaaata gggagtaaaa cgaaagatag gaagattggg cgcattaggg
    27481 tttccttaat cattgaaatc cgctcattca gaaagaacgg gttgggccgg aggggtaatg
    27541 ccgtctgtga aatgaggcaa atatgggaaa ttgatgcagc cttcgtgttt tacgtattta
    27601 tatattccgt atggaggaat atgctgcttt tgaagagtgt attatggatg aactgccgat
    27661 aacagtaaat cggtttgatt tgtttccgtc agcttatgcc gtctgaagcc ctttcagacg
    27721 gcatttattt tgaagttctg gttgtttttg ccagtgatat tttgatggtt tgaagctgtc
    27781 ttttatattc ccggcaatgc gggcagaaca gcaggtgcat atatatggaa atcttctcgc
    27841 ccggggtggt ttcccggtcc tgatgtttgg aaagaagcag ggcaatatcg cggcattttt
    27901 tcataacgtt tacttcgggt tttcttggtt gaaccatttg atttgcaggc attggcgcaa
    27961 tgattctcgg gcgcggtgca taatggtgtg gtagttggac gtgctgatac cgcacatttg
    28021 ttgtatttcg ttggatgaaa aaccgagtat ttccttcagg gtgaataccc gtgcggtgtt
    28081 ttcaggcagg ttgtataggc agctttgcag aattttttgg aattcgttgt tatttaatga
    28141 tttttccgga gtgttccaat gttgcggctg cccttccggc gtccaatgcc cgttttggga
    28201 aaaatggctt tcaaatgctt catccagcag ctcgtcatcc agtgcggtaa agactttcct
    28261 ctgcctcccg atttgacgta atgcgtcaat aattttgttt ttcaatatgg caaaaagcca
    28321 gctgttgaca agtgccctgc cttgaaaact gtcgcctgcg ctgtatgcgg acagcaatgt
    28381 ttcctgcact aaatcttcag ccaaatcagg gtggtcgggc aactggagtc gcgcaaaatg
    28441 cagaagcagt ttgcgcgact ccattaattc ggcatcggtc aggtcgggta gcggcatagt
    28501 atcgggaaca aggtaatttt atgcggatag atggttttgg ctcggggaat tgccttccat
    28561 attttcggat atggcggaca aggaggggat gataatcctt tgttcaatcc agcctgacca
    28621 tttcccaagc aaaatatttt tccaattatc ggctttaggc ataaattcga caagggtttg
    28681 ggcgagggtg tcaaacgaaa gcgcggagga acccattatc tccagcagca tcatatcgaa
    28741 gccgtccaat gtttggtaca tcacatcatc ttcggcgttt cgccatatta acaaggctgt
    28801 ttccgcttcc tgcaaatcat gggtaacatc gtatcgatat tgccggataa aggccgcagg
    28861 ggagggcgtg tatttgctgt catttgaata atgaatgtcg ggaatatccg gaatttgtgc
    28921 aacttctgcc agcaattggg tatattcaaa atccatcagt gccaaaatgc cgtctgaaag
    28981 cggcaggctt tggcaatatt gtaggaactc gccggggatt tcttgaaaat agggcgtttg
    29041 ggcacgcgcg tcgcggacga aaccttcttt cagacggctc cattctttgc tgtcaaagta
    29101 ttgccgcgtt tcggtatagc agcggtcgat aaagctgtgg atattgttgc gtatcaggcg
    29161 gatatagacg ttcaatcggt cttgcggcag accgtctgcg gcttcgcccc cgcgtatggc
    29221 ttgggagaaa cggtgctggt attgggcgga ggtttcaggc tgcacggcgg tattcctttc
    29281 cggcacgcgt ttgataatcg gcaattttgg caacttcggc ttcgagttcg gcaaaaggtg
    29341 ggaaattgaa atcgcgttcc aacagggtgg gagggatggt gggaagcttg gtgtaggcga
    29401 gttcgagcaa gtcccaaacg gtcggtaaaa ccgccgcgcc gtgggtatca atcaaaagct
    29461 cgggggtttc ggcatcgtgt ccggcgatgt ggatgtagca cacgcgccct gcgtccacat
    29521 tctctaaaaa ggcctcaggc gacagcaggc cgtgattgac ggcgttgacg taaatattgt
    29581 tcacatccaa atgaataccg cagtcggctt cacgggcaac cgcgttgagg aactcgacct
    29641 cgttcatctc ggcaagcgga gaatgcaggt agtaggacgt gttttccacg gcgatgcggc
    29701 agcccaaacg gtcttgcact tcgcggatac gccgcgccgt atgatgcacc atttcttcag
    29761 tgaaaggcag cggcaacaaa tcgtaaagat gaccgccgtc gtggcagtag ctcaaatgat
    29821 cggagaaaaa cgtgcagtca taacggcaca tcatttcttt gatgccgtct atcaaatcgg
    29881 tatccagcgg ggcttgtccg cccagcgaca tagataatcc gtgcaacgcc agcggcagcc
    29941 gttccgccac acggtcaaac tgtttgcgcg ccctgccgcc cattttcagc cagttttccg
    30001 gtgcggcttc gataaagcat atcgggctgt tttcggaaag cgagagaaag tcttccgcca
    30061 agtcgcggcg gtagcccaag cctgcgtgtt gaatcatatt tttacccggt tcggatggca
    30121 caaaagaata atgtgcttgt ttaaaaaaat ctttgtttgc cgttaaaaaa gccgcatttt
    30181 actgaaaaac gcggcttggt ttgaagggga gggattattt agaaccgcat ttgccttcgc
    30241 cgcatttgcc ttcggcagat ttggctttgg ctttagatgc tttggtgtgt ttgtgggctt
    30301 tttttacagt tgcaccgcat ttgccctcgc cgcatttgcc ttcgcctgct ttagaagcag
    30361 ccgcgccgca cgaaccttcg gcagatttgg atgcgccgca agagccttgg gcggattttt
    30421 gaacgcctgt tgcgttgctt gccggtttgt gggcggcaac ggcgccggcg gccaaagaca
    30481 gggataaagc accggcgagt gcggcagcaa tatttttgtt catttctatt tccttttcag
    30541 aatatcaagt taacgagata aggttggaat gtttaaagtc ggattccacg actgaatcaa
    30601 tcttgtttca gacggcattt ggggcaaaac agttttttca gcagcgtatc cagcgaccat
    30661 ccgcccgcac cctggaaaag cagcgggatt aataccacga tataaattaa agccatttta
    30721 taaccgttgt cgcagacatt gtaacccgaa ccggcgtgaa ccgcagccca agcgacggcg
    30781 gtaacgacca tcagccccaa tgccgacagg cgcgttgcca aacccaaaag caacagtacg
    30841 ggcagcaaaa gctccgcata catggcgaaa ttccagttta acgcgtccgg cagcaagttg
    30901 aacgggaatg gaaactgatc gttgatttcg gaaaaccaat tctccccgtt ccatttttgc
    30961 aaacccgatt ccaaaaattc atatgcggca aacagacgca aaaccagcat agcggatgcc
    31021 gtctgccaag ttgttccgat tttcataagt gttcctgttt ttgttcctgt tgataccccg
    31081 ttagacggat tttgtcggaa aaactgacac ccgtcagcta ataatttaac aagttaatga
    31141 atataaaaag aaagtatttt gacggaaaga aagggaaaaa ggcgggatat ttggataacg
    31201 gcggtgttgt gagataattc ccgccttgga cggtatgcct tcaaatacaa tttccgtaaa
    31261 ggaaaaaaga aaatattaat tttgatgatg atttaacaaa gcccctttcg cttggctgtc
    31321 aaaggagatt gttaagaaaa gcaatctccc tttgacgggg tgcgatatgt aaaggaatgg
    31381 aaaatatggt aacgttttca aaaatcagat catttttggc aatcgccgcc gccgcgttgc
    31441 ttgccgcctg cggtacggcg ggaaacaatg ccgcccgcaa gccggtgcaa accgccaaac
    31501 ccgccgcagt ggtcgctttg gcactcggtg gcggcgcatc taaaggattt gcccatatag
    31561 gaattgttaa ggttttgaaa gaaaacggta ttcctgtgaa ggtggttacc ggcacatcgg
    31621 caggttcgat agtcggcagc cttttggcat cgggtatgtc gcccgaccgc ctcgaattgg
    31681 aagccgagat tttaggtaaa accgatttag tcgatttaac cttgtccacc agtggtttta
    31741 tcaaaggcga aaagctgcaa aattacatca accgaaaagt cggcggcagg cagattcagc
    31801 agtttcccat caaatttgcc gccgttgcca ctgattttga aaccggcaag gccgtcgctt
    31861 tcaatcaggg gaatgccggg caggcggttc gtgcttccgc cgccattccc aatgtgttcc
    31921 agccagtcat catcggcagg cacaaatatg ttgacggcgg tctgtcgcag cccgtgcccg
    31981 tcagtgccgc tcggcggcag ggggcgaatt tcgtgattgc cgtcgatatt tccgcacgtc
    32041 cgagcaaaaa tgtcggtcaa ggtttcttct cttatctcga tcagacgctg aacgtgatga
    32101 gcgtttccgt gttgcaaaac gagttggggc aggcggatgt ggttatcaaa ccgcaggttt
    32161 tggatttggg tgcagtcggc ggattcgatc agaaaaagcg cgccatccgg ttgggcgagg
    32221 aggcagcacg tgccgcattg cctgaaatca aacgcaaact ggcggcatac cgttattgac
    32281 gctatgccgt ccgaacgtgt ttccagaagg ctttatccat agaaaaaaat acccgaatcc
    32341 cgtacaatac gcccctaaaa ttttgaaaga acgaaagaat catggaagcc gaagtaatca
    32401 accagctcaa caacaccctg aatgatttgg agaagcgtag cgaagacatc cgcgtttata
    32461 tggattatca gggcaagaaa gaccgattgg aagaagtcat cgggctttcc gaagaccccg
    32521 agctttggaa cgacccgaaa cgtgcccaag aaatcggcaa agagagcaaa atccttgaag
    32581 gcatcgtgct gacgctcgac aacatcgctt cgggtatcga agacaaccgt atgctgattg
    32641 aaatggccgt cgaagaaaac gacgaagaag gttttgccgc cgtgaaggaa gacgtggcgg
    32701 ggctggaaaa acagatggcg gatttggagt tcaaacggat gttcaaccag cccgccgacc
    32761 cgaacaactg ctttatcgac atcaccgccg gcgcgggcgg taccgaagcg gaagactggg
    32821 cggggatgtt gttccgtatg tacagccgct atgccgagcg caaaggcttc aaaatcgaaa
    32881 tccttgagga agacgacggc gaaatcgcag gcatcaaccg cgccaccatc cgagtggaag
    32941 gcgaatacgc ttatggtttg ctgcgtaccg aaaccggcgt tcaccgcttg gttcgctact
    33001 cgccgtttga ttcgaacaac aaacgccata cctcgtttgc ctccgtgttc gtttaccctg
    33061 aaatcgacga ttccatcgaa atcgaaatca accccgccga tttgcgcatc gacacctacc
    33121 gtgcatcggg cgcgggcggt cagcacatca acaaaaccga ctccgccgtg cgtattaccc
    33181 acgagccgac ggggattgtg gtgcaatgtc aaaacgaccg ttctcaacac gccaacaaag
    33241 ccgccgcgat ggaaatgttg aaatccaaac tgtatgaatt ggaaatgcgc aaacgcaacg
    33301 aagagaaaca ggcattggaa gaaggtaagt cggatgtggg ttggggcagc caaatccgtt
    33361 cgtatgtttt ggattcctca cgcatcaaag acttgcgtac aggctacgaa gtcggcaaca
    33421 ccaaagccgt attggacggc gacttggacg gctttatcga agccagcctg aaacagggtg
    33481 tgtagtttag gctgtatgcc gtctgaaacc tgtaccgttt cagacggcat ttttgcgttt
    33541 cgtgttataa tccgcgccgc acacgggacg ggcagacagt cgccgcgtat cgcgtaaggc
    33601 atacggggag gaaagtccgg gctccgcagg gtagaatgcc ggttaacggc cgggcgcggt
    33661 aacgcgacgg aaagtggaac agaaagcaaa accgccgatg gctgctttgg cagcacaggc
    33721 aagggtgaaa aggtgcggta agggcgcacc gcgcatttgg taacaatatg cggcaggcca
    33781 aaccccattc ggagcaagac caaacagaac gcaatgacgc tgcccgccga gcgttcgggt
    33841 aggttgcttg agcataccgg caacggtatg cctagaggaa tgactgtccg agacagaacc
    33901 cggcttaccg cctgtccggt gtgcatccga cacgcaaatg ccgtctgaaa cctgaaatac
    33961 ggttcggacg gcatttttat ttaggctgtg tttttatatt ccgttgatgc agccctgatg
    34021 agtcggacaa aatgcctttt actcaatagg accggtttcc gcccaagcgg aaataaacgg
    34081 ctatttccct tgcaaacaga cggaatcagg cgggtattta aatgacaatc ggcaggctgc
    34141 cttccggttg ggcgggagcc tgtcctgtcg ggtggaaacg gtttggtttc attatgcgga
    34201 aaaatgccgt ctgaagggtt cagacggcat cggcgcatcg gggcaggact tagtctgtgc
    34261 cttcgagcat tttgttcaat ttggggacga gcaggagcag caggaagcct gtggctgcgc
    34321 cgatgtagaa cagcagccgg tagaagccga tttcgtcgcc cgcctgatag cctttttcaa
    34381 acaatacgcc gcccaaagtg aagcctaatg aaaaggcaag gaaattaagg gcgaccattt
    34441 gggttttgaa taaaggcggt gcgattttgg tggagatgga cagcgcaatc ggggaaatca
    34501 tcagttcgcc tatcgtgatg gcgaggacga tcagtgcgaa aaccgcaata ggcatcggcg
    34561 ttccggagga aataaaggga acgaatccca aaaacgacgc gccggtaaca aataccgcca
    34621 tagcgaattt cagcggggtt ttgggttgtt tgcgccccat ttttgtccac attgccgcca
    34681 tgagtccgga aaacaggatg acccacaggc tttgcataga atctttccag gcgacgggta
    34741 cggtaaacga accgatggtg cggttgacgg tttcgtcgaa atagacggtt gccacggtgt
    34801 aaatctgaaa ccagacggcc caaaacatac agatggtcag gaaaagcggg atgtaggcga
    34861 tgatgtgccg tttgttgtcg gaactgacgc gggggttggt cagcaggcgg gcgaaatagg
    34921 cgatgacggc aaggatgacg gtagataaga ggatgccgga gaaattgtcg aggttgacaa
    34981 gcccggtttt gatggcggtt gcaagtgcgg cgatgagggt gatgccgacg gcggccgcag
    35041 ttttgccctg tccttttgaa agcggatggg ggacggtggg gtggggcagg tttttacgtc
    35101 ccaaggaata acgccacaag ccgaatgcca taccgaccgc cgccgcgccg aaaccataat
    35161 ggaaaccgat gttttcttga agcagtccgg tcaataaggg gcctaagaag ccgccgatat
    35221 tgatggcgat gtagaaaatg gaaaatcccg catcgcgcag cgggcgcatt tcgtcctgtt
    35281 cgtataatgc gcccaccata gaactggccg tagatttcac gccgccgctg cccaatgcga
    35341 tgaatatcag cccgattaga aggccgtaca ggcccggtgc ggcggcaagg acgatgtgcc
    35401 cgagcattac gacgatgccc gagaggaaga gggttttttc cgcaccccat acgcggtcgg
    35461 caaaccacgc gcccaaaatg gtggacaggt acacgctgcc gctgtatgcg ccgacaatgc
    35521 cgcctgcgag ggttttgtct atgcccaagc cgcctttgtc ggcggtgtag tagaggtaaa
    35581 tcagcaggat gccctgcatt ccgtaaaatg aaaaacgttc ccacaattcg atatggaaga
    35641 gggtggaaag ctggaagggg tggccgaaaa atgttttttc tccggtgcgg gcgggatgcc
    35701 gggacataaa ggttctccta aaagattttc tctcaatacc gtctgaaacg gatgcggacg
    35761 gcggcggtgt ttttttgtat gaaaatatcg gtttttaacc gatattttca ttctttgtca
    35821 aacgacgcgc tgccgttttt cgcgggcgga tgtttttata tttgtttcaa ttcaatggat
    35881 tgtattttag aggacgtgtt ccgatacggc gcgggtaaat ccttttctgt caatggctta
    35941 tccgataggg cggtttttac ttagatggga aatcacttcc ttatatatcc gccacgccga
    36001 agggcgcatt atgg
//
LOCUS       DAWTQS010000015        34698 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.015, whole genome shotgun sequence.
ACCESSION   DAWTQS010000015 DAWTQS010000000
VERSION     DAWTQS010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 34698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34698
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.015"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(19..1329)
                     /locus_tag="WNA25_000741"
     CDS             complement(19..1329)
                     /locus_tag="WNA25_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFA9823550.1"
                     /translation="MDISKQTLLDRVFNLKANGTTVRTELMAGLTTFLTMCYIVIVNP
                     LILGETGMDMGAVFVATCIASAIGCFVMGFIGNYPIALAPGMGLNAYFTFAVVKGMGV
                     PWQVALGAVFISGLIFILFSFFKVREMLVNALPMGLKMSIAAGIGLFLALISLKGAGI
                     IVANPATLVGLGDIHQPSALLALFGFVMVVVLGYFRVQGAIIITILTITVIASLMGLN
                     EFHGVVGEVPGIAPTFMQMDFKGLFTVSMVSVIFVFFLVDLFDSTGTLVGVSHRAGLL
                     VDGKLPRLKRALLADSTAIVAGAALGTSSTTPYVESAAGVSAGGRTGLTAVTVGVLML
                     ACLMFSPLAKSVPVFATAPALLYVGTQMLRSARDIDWDDMTEAAPAFLTIVFMPFTYS
                     IADGIAFGFISYAVVKLLCRRTGDVPPMVWVVAVLWALKFWYLG"
     gene            complement(1465..2643)
                     /gene="ftsZ"
                     /locus_tag="WNA25_000742"
     CDS             complement(1465..2643)
                     /gene="ftsZ"
                     /locus_tag="WNA25_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFA9823551.1"
                     /translation="MEFVYDVAESAVSPAVIKVIGLGGGGCNAINNMVANNVRSVEFI
                     SANTDAQSLAKNHAAKRIQLGTNLTRGLGAGANPDIGRAAAQEDREAIEEAIRGANML
                     FITTGMGGGTGTGSAPVVAEIAKSLGILTVAVVTRPFSYEGKRVHVAQAGLEQLKEHV
                     DSLIIIPNDKLMTALGEDVTMREAFRAADNVLRDAVAGISEVVTCPSEIINLDFADVK
                     TVMSNRGIAMMGSGYAQGIDRARMATDQAISSPLLDDVTLDGARGVLVNITTAPGCLK
                     MSELSEVMKIVNQSAHPDLECKFGAAEDETMSEDAIRITIIATGLKEKGAVDPTPARE
                     VEAVAPSKQEQSHNVEGMIRTNRGIRTMNLTAADFDNQSVLDDFEIPAILRRQHNSDK
                     "
     gene            complement(2762..4006)
                     /gene="ftsA"
                     /locus_tag="WNA25_000743"
     CDS             complement(2762..4006)
                     /gene="ftsA"
                     /locus_tag="WNA25_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFA9823552.1"
                     /translation="MEQQKRYISVLDIGTSKVLALIGEVQDDDKINIIGLGQAPSRGL
                     RAGMVTNIDATVQAIRQAVNDAELMADTKITHVTTGIAGNHIRSLNSQGVVKIKDGEV
                     TQADIDRAIETAKAINIPPDQKILDAVVQDYIIDTQLGVREPIGMSGVRLDTRVHIIT
                     GASTAVQNVQKCIELCGLKSDQIMLQPLASGQAVLTEDEKDLGVCVIDIGGGTTDIAV
                     YMNGAIRHTSVIPAGGNLITKDLSKSLRTPLDAAEYIKIHYGVASCDTEGLGEMIEVP
                     GVGDRTSRQVSSKVLAAIISARIQEIFGVVLGELQKSGFPKEVLNAGIVLTGGVSMMT
                     GIVEFAEKIFDLPVRTGAPQEMGGLSDRVRTPRFSTAIGLLHAACKLEGNLPQPENGT
                     VQEREGGGGLLARLKRWIENNL"
     gene            complement(4092..4820)
                     /locus_tag="WNA25_000744"
     CDS             complement(4092..4820)
                     /locus_tag="WNA25_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFA9823553.1"
                     /translation="MWDNAEAMERLTRWLLVMMAMLLAASGLVWFYNSNHLPVKQVSL
                     KGNLVYSDKKALGSLAKEYIHGNILRTDINGAQEAYRRYPWIASVMVRRRFPDTVEVV
                     LTERKPVARWGDHALVDGEGNVFEARLDRPGMPVFRGAEGTSAEMLRRYDEFSTVLAK
                     QGLGIKEMTYTARSAWNVVLDNGITVRLGRENEMKRLRLFTEAWQHLLRKNKNRLSYV
                     DMRYKDGFSVRHAPDGLPEKESEE"
     gene            complement(4810..5724)
                     /locus_tag="WNA25_000745"
     CDS             complement(4810..5724)
                     /locus_tag="WNA25_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFA9823554.1"
                     /translation="MQNFGKVAVLMGGFSSEREISLDSGTAILNALKSKGIDAYAFDP
                     KETPLSELKERGFQTAFNILHGTYGEDGAVQGALELLGIPYTGSGVAASAIGMDKYRC
                     KLIWQALGLPVPEFAVLYDDTDFDAVEEKLGLPMFVKPAAEGSSVGVVKVKEKGRLKS
                     VYEELKHLQGEIIAERFIGGGEYSCPVLNGKGLPGIHIIPATEFYDYEAKYNRDDTIY
                     QCPSEDLTEAEESLMRELAVRGAQAIGAEGCVRVDFLKDTDGKLYLLEINTLPGMTGH
                     SLVPKSAAVTGVGFADLCIEILKAAHVG"
     gene            complement(5836..7245)
                     /locus_tag="WNA25_000746"
     CDS             complement(5836..7245)
                     /locus_tag="WNA25_000746"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFA9823555.1"
                     /translation="MMKNRVSNIHFVGIGGVGMSGIAEVLHNLGFKVSGSDQARNAAT
                     EHLSSLGIQVYPGHTAEHVNGADVVVASTAVKKENPEVVAALERQIPVIPRALMLAEL
                     MRFRDGIAIAGTHGKTTTTSLTASILGAAGLDPTFVIGGKLNAAGTNARLGKGEYIVA
                     EADESDASFLHLTPIMSVVTNIDEDHMDTYGHSVEKLHQAFIDFIHRMPFYGKAFLCV
                     DSEHVRAILPKVSKPYATYGLDDTADIYATDIENVGAQMKFTVHVQMKGHEQGSFEVV
                     LNMPGRHNVLNALAAIGVALEVGASVEAIQKGLLGFEGVGRRFQKYGDIKLPNGGTAL
                     LVDDYGHHPVEMAATLAAARGAYPEKRLVLAFQPHRYTRTRDLFEDFTKVLNTVDALV
                     LTEVYAAGEEPVAAADSRALARAIRVLGKLEPIYCENVADLPQMLMNVLQDGDVVLNM
                     GAGSINRVPSALLELSKQI"
     gene            complement(7400..8467)
                     /gene="murG"
                     /locus_tag="WNA25_000747"
     CDS             complement(7400..8467)
                     /gene="murG"
                     /locus_tag="WNA25_000747"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFA9823556.1"
                     /translation="MGGKTFMLMAGGTGGHIFPALAVADSLRVRGHHVIWLGSKDSME
                     ERIVPQYGIRLETLAIKGIRGNGIKRKLMLPFTLYKTVREAQRIIRKHRVECVIGFGG
                     FVTFPGGLAAKLLGVPIVIHEQNAVAGLSNRHLSRWAKRVLYAFPKAFSHEGGLVGNP
                     VRADISNLPVPAERFQGREGRLKILVVGGSLGADVLNKTVPQALALLPEEVRPQMYHQ
                     SGRNKLGNLQADYDALGVKAECVEFITDMVSAYRDADLVICRAGALTIAELTAAGLGA
                     LLVPYPHAVDDHQTANARFMVQAEAGLLLPQTQLTAEKLAEILGSLNREKCLKWAENA
                     RTLALPHSADDVAEAAIACAA"
     gene            complement(8471..9742)
                     /locus_tag="WNA25_000748"
     CDS             complement(8471..9742)
                     /locus_tag="WNA25_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFA9823557.1"
                     /translation="MGDGVHTLLLDRPIVRDGRKFDAPLLWMVVLMTAFGLLMIYSAS
                     VYLASKEGGDQFFYLTRQAGFVVAGLIASGFLWFLCRMRTWRRLVPWIFALSGLLLVA
                     VLIAGREINGATRWIPLGPLNFQPTELFKLAVILYLASLFTRREEVLRSMESLGWQSI
                     WRGTANLIMSATNPQARRETLEMYGRFRAIILPIMLVAFGLVLIMVQPDFGSFVVITV
                     ITVGMLFLAGLPWKYFFVLVGSVLGGMVLMITAAPYRVQRVVAFLDPWKDPQGAGYQL
                     THSLMAIGRGEWFGMGLGASLSKRGFLPEAHTDFIFAIIAEEFGFFGMCVLIFCYGWL
                     VVRAFSIGKQSRDLGLTFNAYIASGIGIWIGIQSFFNIGVNIGALPTKGLTLPLMSYG
                     GSSVFFMLISMMLLLRIDYENRRKMRGYRVE"
     gene            complement(9921..11258)
                     /locus_tag="WNA25_000749"
     CDS             complement(9921..11258)
                     /locus_tag="WNA25_000749"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFA9823558.1"
                     /translation="MTFQNKKTLVAGLGGTGISMIAYLRKNGAEVAAYDAELKAERVA
                     QIGKMFDGLVFYTGRLKDALDNGFDILALSPGISERQPDIEAFKQNGGRVLGDIELLA
                     DIVNRRGDKVIAITGSNGKTTVTSLVGYLCIKCGLDTVIAGNIGTPVLEAELQREGKK
                     ADVWVLELSSFQLENTESLRPTAATVMNISEDHLDRYDDLLDYAHTKAKIFRGDGVQV
                     LNADDVFCRAMKRAGREVKRFSLEHEADFWLERGTGCLKQGNEDLISTQDIPLQGLHN
                     AANVMAAVALCEAVGLPREALLEHVKTFQGLPHRVEKIGEKNGVVFIDDSKGTNVGAT
                     AAAIAGLQNPLFVILGGMGKGQDFTPLRDALKDKAKGVFLIGVDAPQIRRDLDGCGLN
                     LTDCVTLEEAVQTAYAQAEAGDIVLLSPACASFDMFKGYAHRSEVFIEAFKAL"
     gene            complement(11343..11723)
                     /locus_tag="WNA25_000750"
     CDS             complement(11343..11723)
                     /locus_tag="WNA25_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HFA9823559.1"
                     /translation="MKQTVKWLAAALIALGLNQAVWAGDVSDFRENLQAAEQGNAAAQ
                     FNLGVMYENGQGVRQDYVQAVQWYRKASEQGDAQAQYNLGLMYYDGRGVRQDLALAQQ
                     WLGKACQNGDQNSCDNDQRLKAGY"
     gene            complement(11848..12930)
                     /locus_tag="WNA25_000751"
     CDS             complement(11848..12930)
                     /locus_tag="WNA25_000751"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFA9823560.1"
                     /translation="MFLWLAHFSNWLTGLNIFQYTTFRAVMAALTALAFSLMFGPWTI
                     RRLTALKCGQAVRTDGPQTHLVKNGTPTMGGSLILTAITVSTLLWGNWANPYIWILLG
                     VLLATGALGFYDDWRKVVYKDPNGVSAKFKMVWQSSVAVIAGLALFYLAANSANNILI
                     VPFFKQIALPLGVVGFLVLSYLTIVGTSNAVNLTDGLDGLAAFPVVLVAAGLAIFAYV
                     SGHYQFSQYLQLPYVAGANEVAIFCTAMCGACLGFLWFNAYPAQVFMGDVGALALGAA
                     LGTVAVIVRQEFVLVIMGGLFVVEAVSVMLQVGWYKKTKKRIFLMAPIHHHYEQKGWK
                     ETQVVVRFWIITIVLVLIGLSTLKIR"
     gene            complement(13175..13351)
                     /locus_tag="WNA25_000752"
     CDS             complement(13175..13351)
                     /locus_tag="WNA25_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823561.1"
                     /translation="MKSRRFFKALLLIAALVGAFYAGMRTQAYLYEDLCLDLGGGKNP
                     GSYPICVIGKVPAR"
     gene            complement(13354..14712)
                     /locus_tag="WNA25_000753"
     CDS             complement(13354..14712)
                     /locus_tag="WNA25_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFA9823562.1"
                     /translation="MKPLDLNFICQALKLPMPSENKPVSRIVTDSRDIREGDVFFALA
                     GGRFDAHDFVGGVLSAGAAAVVVSREDCAALGGALKVDDTLAALQTLAKAWRDNVNPF
                     VFGITGSGGKTTVKEMLAAVLRRRFGDDAVSATAGNFNNHIGLPLTLLKLNEKHRYAV
                     IEMGMNHFGELAVLTQIAKPDAALVNNALRAHVGCGFDGVGDIAKAKSEIYAGLCSDG
                     MALIPQEDANMAVFKTATFNLNTCTFGVDSGDVRAENIVLKPLSCEFDLVCGDERTAV
                     VLPVPGRHNVHNAAAAAALALAAGLSLNDVAEGLQGFSNIKGRLNVKAGIKGATLIDD
                     TYNANPDSMKAAVDVLARMPAPRIFVMGDMGELGEDEAAAMHAEVGAYARDQGIEAAY
                     FVGDNSVEAAEKFGADGLWFAAKDPLIQVLSHDLPERATVLVKGSRFMQMEEVVEALE
                     DK"
     gene            complement(14837..16483)
                     /locus_tag="WNA25_000754"
     CDS             complement(14837..16483)
                     /locus_tag="WNA25_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFA9823563.1"
                     /translation="MKQSARIKNMDQTLKNTLGICALLAFCFGAAIASGYHLEYEYGY
                     RYSAVGALASVVFLLLLARGFPRVSSVVLLIYVGTTALYLPVGWLYGAPSYQIVGSIL
                     ESNPAEAREFVGNLPGSLYFVQALFFIFGLTVWKYCVSVGVFADVKNYKRRSKIWLTI
                     LLTLILSCAVMEKIAGDKDWREPDAGLLLNIFDLYYDLASAPAQYAAKRAHILEAAKK
                     ASTWHIRHVAPKYKNYVVVIGESARSDYMNVYGFPLPDTPFLSRTKGLLINGYQSTAH
                     ATNLSLPQTLGLPGEPNNNIVSLAKQAGFRTAWLSNQGMLGHFANEISTYALRSDYPW
                     FTQRGDYGKSAGLSDRLLLPAFKRVLTGNAGTKPRLIVMHLMGSHSDFCTRLDKDARR
                     FQYQTEKISCYVSTIAQTDKFLEDTVKILNENKESWSLVYFSDHGLMHVGKGGERTLT
                     HGEWKRQSYGVPLVKISSDDTRREMIKVRRSAFNFLRGFGSWTGIETDELPDDGYDFW
                     GNVPDVPGEGNNLAFIDRQSDDPAPWYAGKGKSAKNTSKK"
     gene            complement(16506..17984)
                     /locus_tag="WNA25_000755"
     CDS             complement(16506..17984)
                     /locus_tag="WNA25_000755"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFA9823564.1"
                     /translation="MFSKLSPLAETGIPTLSCANAAGRLLHSDSRQIKQGDIFVACQG
                     EYTDGRSYIPAAVANGAAFVFWDDDGRFAWNPEWKVPNQGIKDLKHRAGILAAQVYGN
                     VSDGLKVWGVTGTNGKTSITQWLAQAADLLGEKTAIIGTVGNGFWGALEETAHTTPAP
                     VDVQTLLYRFRQQGATAAAMEVSSHGLDQSRVNGVPFRSAIFTNLTRDHLDYHGTMEA
                     YGAIKSRLFYWHGLKHAVINTDDGYGAELAGRLKKDCPDLAVYSYGFSEHADIRITDF
                     TASSDGIEAVFRTPWGEGKCRTRLLGRFNAQNLAACIALLCANGYPLDKVLDVLAKIR
                     PASGRMDCIMNSGKPLVVVDYAHTPDALEKALSTLQEIKPQGAALWCVFGCGGNRDCG
                     KRPLMGAAAVQGADKVVVTSDNPRLENPQDIINDILPAVPAPECVEADRAAAIRYAVE
                     QAAANDIILIAGKGHENYQDVQGVKHRFSDLEIVGQALLTRK"
     gene            complement(18009..19757)
                     /locus_tag="WNA25_000756"
     CDS             complement(18009..19757)
                     /locus_tag="WNA25_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HFA9823565.1"
                     /translation="MLIKSEYKPRMLPKEEQVKKPMTSNGRISFVLMAMAVLFACLIA
                     RGLYLQTVTYNFLKEQGDNRIVRTQALPATRGTVSDRNGAVLALSAPTESLFAVPKDM
                     KEMPSAAQLERLSELVDVPVDVLRNKLEQKGKSFIWIKRQLDPKVAEEVKALGLENFV
                     FEKELKRHYPMGNLFAHVIGFTDIDGKGQEGLELSLEDSLYGEDGAEVVLRDRQGNIV
                     DSLDSPRNKAPQNGKDIILSLDQRIQTLAYEELNKAVEYHQAKAGTVVVLDARTGEIL
                     ALANTPAYDPNRPGRADSEQRRNRAVTDMIEPGSAIKPFVIAKALDAGKTDLNERLNT
                     QPYKIGPSPVRDDTHVYPSLDVRGIMQKSSNVGTSKLSARFGAEEMYDFYHELGIGVR
                     MHSGFPGETAGLLRNWRRWRPIEQATMSFGYGLQLSLLQLARAYTALTHDGVLLPLSF
                     EKQAVAPQGKRIFKESTAREVRNLMVSVTEPGGTGTAGAVDGFDVGAKTGTARKLVNG
                     RYVDNKHVGTFIGFAPAKNPRVIVAVTIDEPTAHGYYGGVVAGPPFKKIMGGSLNILG
                     ISPTKPLTAAAVKTPS"
     gene            complement(19818..20081)
                     /gene="ftsL"
                     /locus_tag="WNA25_000757"
     CDS             complement(19818..20081)
                     /gene="ftsL"
                     /locus_tag="WNA25_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFA9823566.1"
                     /translation="MNKSNFFLLLAVCVSAFSVVMQQNQYRLNFTALDKAKKQEIALE
                     QDYAQMRVQQARLANHEAIRAAAEKQNLHPPVSGNTFMVEHQR"
     gene            complement(20108..21064)
                     /gene="rsmH"
                     /locus_tag="WNA25_000758"
     CDS             complement(20108..21064)
                     /gene="rsmH"
                     /locus_tag="WNA25_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFA9823567.1"
                     /translation="MSGAESYRHITVLLNEAVDALAVREDGVYVDGTFGRGGHSRLIL
                     SRLGDAGRLIVFDKDPQAIAVAEELARSDKRVGVVHGGFASFQTALDGLGIGKVDGAL
                     FDLGISSPQIDDGSRGFSFRFDAPLDMRMDTTRGMSAAEWIAVASEQDLHEVIKNYGE
                     ERFSRQIVRAIVAQRAESPIDTTRKLAQIVAQNVRTRERGQDPATRTFQAIRIFINRE
                     LEEVGAVLPQVMCRLKEGGRLAVIAFHSLEDRIVKQFVKKYSQHEPLPSWAAVREADL
                     PEPPLKIVGRALKPGEAEIAANPRARSAVLRVAERTAGPIPE"
     gene            complement(21061..21516)
                     /gene="mraZ"
                     /locus_tag="WNA25_000759"
     CDS             complement(21061..21516)
                     /gene="mraZ"
                     /locus_tag="WNA25_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFA9823568.1"
                     /translation="MFGGAHELSIDSKGRLAVPAKFRDILSRLYTPAVVATLESKHKL
                     LMYPVAEWEKVAAQLLNLKVADNPVLRRFQNLLLHNAEILEWDSAGRVLVPAGLRKRV
                     DFDREVVLVGRANRLELWGREQWEAEMVQALDDDPDELAFQLSQTDLQL"
     gene            21795..23042
                     /locus_tag="WNA25_000760"
     CDS             21795..23042
                     /locus_tag="WNA25_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFA9823569.1"
                     /translation="MTAPVPTCNMSGNPINLHKLTFIMLLPSPEARQSSLNLQTLIAE
                     EIGKHGNWIPFSRFMELVLYAPQYGYYTGGSHKIGNTGDFITAPTLTPLFAQTLARQL
                     QELLPQTAGNIYEFGAGTGQLAADLLGSVSDSINCYYIIEISPELAARQKNLIQARAP
                     EASQKVVHLTALPEAFDGIIIGNELLDAIPVEIVRKNEGGLLEHIGVCTDNGRFAYSA
                     RPLHDPSLSTSASLYFPQTDYPYTSELHPQQYAFIRTLASRLERGGMIFIDYGFDAAQ
                     YYHPQRNQGTLIGHYRHHVIHNPFDFIGLADLTAHVNFTDIAQAGTDAGLDLTGYLPQ
                     SHFLLNLGITELLAQTGKTDSAAYIREAAAVQKLIDQHEMGELFKVIAFGKNIGIDWA
                     GFRFGDICHKLQPSCRLNPLQTA"
     gene            23138..23214
                     /locus_tag="WNA25_000761"
     tRNA            23138..23214
                     /locus_tag="WNA25_000761"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23172..23174,aa:Met,seq:cat)
     gene            23222..23297
                     /locus_tag="WNA25_000762"
     tRNA            23222..23297
                     /locus_tag="WNA25_000762"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23255..23257,aa:Ala,seq:ggc)
     gene            complement(23483..24304)
                     /locus_tag="WNA25_000763"
     CDS             complement(23483..24304)
                     /locus_tag="WNA25_000763"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFA9823570.1"
                     /translation="MDFLIVLKALMMGLVEGFTEFLPISSTGHLIVFGNLIGFHSNHK
                     VFEIAIQLGAVLAVVFEYRQRFSNVLHGVGKDRKANRFVLNLAIAFIPAAVMGLLFDK
                     QIKEYLFNPLSVAVMLVLGGFFILWVEKRQSRAEPKIADVDALRPIDALMIGVAQVFA
                     LVPGTSRSGSTVMGGMLWGIERKTATEFSFFLAVPMMVAATAYDVLKHYRFFTLHDVG
                     LILIGFIAAFVSGLVAVKALLKFVSKKNYIPFAYYRIVFGIVIIILWLSGWISWE"
     gene            complement(24337..24981)
                     /locus_tag="WNA25_000764"
     CDS             complement(24337..24981)
                     /locus_tag="WNA25_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFA9823571.1"
                     /translation="MKFKHLLPLLLSAVLSAQAYALTEGEDYLVLDKPIPQEQPGKIE
                     VLEFFGYFCVHCHHFDPLLLKLGKALPSDTYLRTEHVVWRPEMLGLARMAAAVKLSGL
                     KYQANSAVFKAVYEQKIRLENRAVAGKWALSQKGFDGKKLMRAYDSPEAAAVALKMQK
                     LTEQYGIDSTPTVIVGGKYRVIFNNGFDGGVHTIKELVAKVREERKRQTPAVQK"
     gene            complement(24984..25847)
                     /gene="ftsN"
                     /locus_tag="WNA25_000765"
     CDS             complement(24984..25847)
                     /gene="ftsN"
                     /locus_tag="WNA25_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFA9823572.1"
                     /translation="MNKFSQSGKGLSGFFFGLILATVIIAGILLYLNQGGQNAFKIPA
                     PSKQPAETEILKLKNQPKEDIQPEPADQNALSEPDVAKEAEQSDAEKAADKQPVADKA
                     DEVEEKAGEPEREEPDGQAVRKKALTEEREQTVREKAQKKDAETVKKQAVKPSKETEK
                     KASKEEKKAAKEKVAPKPTPEQILNSGSIEKARSAAAKEVQKMKTFGKAEATHYLQMG
                     AYADRRSAEGQRAKLAILGISSEVVGYQAGHKTLYRVQSGNMSADAVKKMQDELKKHG
                     VASLIRAIEGK"
     gene            complement(25953..27449)
                     /locus_tag="WNA25_000766"
     CDS             complement(25953..27449)
                     /locus_tag="WNA25_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFA9823573.1"
                     /translation="MSLALVYSRALSGMNAPLVEVEAHLANGLPHFNIVGLPDTEVKE
                     SRDRVRAAIIQSGFEFPAKKITVNLAPADLPKESGRFDLPIAIGILAASGQVAPEKLA
                     EYEFAGELALSGLLRPVRGALAMAWQGMQAKRAFVLPEENAGQAAVMRGITVYGARSL
                     GEVAAHLNGIEPLAQTECSVPQMPSEHGGQPDLCDVKGQHSARLALEIAAAGGHSLLM
                     MGPPGTGKSMLSQRLPGILPPLAEDELVEVWALRSLLPNHQQQLDSNRPFRSPHHGAS
                     AAAMVGGGSDPRPGEISLAHHGVLFLDELPEFDRKVLEVLREPLENGEIHISRAARQA
                     VYPAKFQLVAAMNPCPCGYLGHPVKPCRCTPESVARYRSKISGPLLDRIDLTIEVPSL
                     SAAELMQQEAGESSASVLERVIAARGKQYARQGKVNAALSVSELDSQACIQKEAQEAL
                     GSLLEKLSLSARSFHRIMRVARTLADLAGDEEVGRSHVMKAIGFRRAL"
     gene            complement(27463..27783)
                     /locus_tag="WNA25_000767"
     CDS             complement(27463..27783)
                     /locus_tag="WNA25_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003763371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HFA9823574.1"
                     /translation="MFGKQLFEEVGSKISETIANSPAKDVEKNIKAMLGGAFNRMDLV
                     TREEFDIQQQVLIKTRTKLAALEARLAKLEAAQNPERAALEAAEAAAEEAVAEIRQQT
                     EAGE"
     gene            28271..29797
                     /gene="putP"
                     /locus_tag="WNA25_000768"
     CDS             28271..29797
                     /gene="putP"
                     /locus_tag="WNA25_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFA9823575.1"
                     /translation="MNPMYITFAIYLVAVLLIGLAAYFSTRNFDDYILGGRSLGPFVT
                     AMSAGASDMSGWLLMGLPGAIYLSGLNEAWIAIGLLVGAYFNWLLVAGRLRVHTEYAN
                     NALTLPDYFFHRFGAGGHLMKVVSALIILFFFTIYCASGIVAGATLFQSLFEGMTYNQ
                     AMWLGAGATIAYTFLGGFLAVSWTDTLQASLMIFALILTPVMVYLGLGGAEQMSAAIQ
                     SVAAGTGKEYGSLFAGTTVIGIISTAAWGLGYFGQPHILARFMAAESAKSLVSARRIG
                     MTWMALCLAGAVAVGYFGIAYFGANPDKVSSMSGNHERIFIALSTLLFNPWIAGIILS
                     AILAAVMSTLSCQLLVCSSAITEDFYKGFLRKNAQQSELVWVGRLMVLAIAVISILIA
                     SDPNSKVLGLVSYAWAGFGAAFGPIVILSVLWKRITAYGALSGMVAGASTVVVWAEWV
                     KKPARAAGESGLLTMYEIVPGFIVCLIVAVLVSLFNKEPSREIQERFEKADADYRAAR
                     "
     gene            30049..33654
                     /gene="putA"
                     /locus_tag="WNA25_000769"
     CDS             30049..33654
                     /gene="putA"
                     /locus_tag="WNA25_000769"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HFA9823576.1"
                     /translation="MFHFAFPAQTALRQAITDAYRRNEIEAVQDMLQRAQMSDEERNA
                     ASELARRLVTQVRAGRTKAGGVDALMHEFSLSSEEGIALMCLAEALLRIPDNATRDRL
                     IADKISDGNWKSHLNNSPSLFVNAAAWGLLITGKLTATNDKQMSSALGRLIGKGGAPL
                     IRQGVNYAMRLLGKQFVTGQTIEEALQNGKEREKMGYRFSFDMLGEAAYTQADADRYY
                     RDYVEAIHAIGKDAAGQGVYEGNGISVKLSAIHPRYSRAQHGRVMGELLPRLKELFLL
                     GKKYDIGINIDAEEANRLELSLDLMEALVSDPDLAGYKGIGFVVQAYQKRCPFVIDYL
                     IDLARRNNQKLMIRLVKGAYWDSEIKWAQVDGLNGYPTYTRKVHTDISYLACARKLLS
                     AQDAVFPQFATHNAYTLGAIYQMGKGKDFEHQCLHGMGETLYDQVVGPQNLGRRVRVY
                     APVGTHETLLAYLVRRLLENGANSSFVNQIVDENISIDRLIKSPFDTIAEQGIHLHNA
                     LPLPRDLYGKCRLNSQGVDLSNENVLQQLQEQMNKAAAQDFHAASIVNGKARDVGEAQ
                     PIKNPADHGDVVGTVSFADAALAQKAVGAAVAAFPEWSATPAAERAACLRRFADLLEQ
                     HTPALMMLAVREAGKTLNNAIAEVREAVDFCRYYANEAEHTLPQDAKAVGAIVAISPW
                     NFPLAIFTGEVVSALAAGNTVIAKPAEQTSLIAGYAVSLMHEAGIPTSALQLILGAGD
                     TGAALTNDASIGGVIFTGSTEVARLINKALAKRGDNPVLIAETGGQNAMIVDSTALAE
                     QVCADVLNSAFDSAGQRCSALRILCVQEDVADRMLDMIKGAMDELVVGKPIQLTTDVG
                     PVIDAEAQQNLLNHINKMKGVAKSYHEVKAAADVDSEKSTFVRPILFELNNLNELQRE
                     VFGPVLHVVRYRADELDSVIDQINSKGYALTHGVHSRIEGTVRHIRSRIEAGNVYVNR
                     NIVGAVVGVQPFGGHGLSGTGPKAGGSFYLQKLTRIPEWVAPTLSQIGQADEAALKRL
                     EALIHKLPFNAEEKKAAAAALGHARIRTLRRAETVLTGPTGERNSISWHAPKRVWIHG
                     GSTVQAFAALTGLAASGVQAVVEPDSPLASYTADLDGLLQISGKPENTGVNHVAALSP
                     LDSSRKQELANRDGALIRILPSGQGLDILQVFEEISCSINTTAAGGNAGLMAVAD"
     gene            33925..34695
                     /locus_tag="WNA25_000770"
     CDS             33925..34695
                     /locus_tag="WNA25_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HFA9823577.1"
                     /translation="MNPAPKNFSSLLFSSLLFSSLLFSSAAQAASEGNGRGPYVQADL
                     AYAAERITHDYPKPTDPSKGKISTVSDYFRNIRTHSIHPRVSVGYDFGGWRIAADYAR
                     YRKWNNSKYSVSIKELKNNNKKKTENQENGSFHAVSSLGLSAVYDFKLNDKFKPYIGA
                     RVAYGHVRHSIDSTKKITAGAGGAGSPVRPSYKSTQDAHHQSNSIRRVGLGVIAGVGF
                     DITPKLTLDTGYRYHNWGRLENTRFKTHEASLGVRYRF"
BASE COUNT         8167 a        10011 c         8592 g         7928 t
ORIGIN      
        1 tttttaatat cgaatcaatc agcccaaata ccagaatttc aatgcccaca atacggcaac
       61 aacccatacc ataggcggca cgtccccagt ccggcgacac aaaagtttga ccacggcata
      121 gctgatgaag ccgaaggcga tgccgtctgc aatcgaatag gtaaacggca tgaagacaat
      181 ggtcaggaac gcgggcgcgg cttcagtcat atcgtcccaa tcaatgtccc tcgcactgcg
      241 gagcatctgc gtgccgacat aaagcagtgc gggcgcggtg gcaaataccg gaacactttt
      301 cgccaatggg gagaacatca gacacgccag catcaatacg ccgacggtaa ccgccgtcag
      361 gccggtccgt ccgcctgccg atacgcccgc cgcgctttcc acataaggcg tggttgaaga
      421 agtacccaaa gccgcacctg ccacaatggc ggtagagtct gcaagcagtg cgcgtttcag
      481 gcggggcagc ttaccgtcca ccagcagtcc ggcacggtgg gatacgccga ccagcgttcc
      541 ggtactgtca aacaaatcga ccaagaagaa gacgaaaatc acgctgacca tgctgacggt
      601 aaacagacct ttaaaatcca tctgcataaa ggtcggcgca atgcccggta cttcgccgac
      661 cacgccgtga aactcgttca aacccatcag gctggcaatg acggtaatcg tcagaatggt
      721 gatgatgatt gcgccttgaa cgcggaaata ccccaatacg accaccatga caaaaccgaa
      781 caatgccaac agtgcgctgg gctgatgaat atcgcccaag ccgaccaagg ttgccggatt
      841 ggcaacgata atgcctgcgc ctttcaggga aatcagtgcc aaaaacaaac cgataccggc
      901 ggcaatcgac attttcaaac ccataggcag tgcgttgacc agcatttccc tgactttaaa
      961 aaagctgaac aggatgaaaa tcagaccgga aatgaacacc gcacccaacg ccacctgcca
     1021 aggcacgccc atacccttaa cgacggcaaa ggtgaaatag gcattcagcc ccatccccgg
     1081 ggcaagcgca atcggatagt tgccgataaa acccatgaca aaacagccga tggcggatgc
     1141 gatacaggtg gcgacgaata ccgcccccat atccattccg gtctcgccca aaatcagggg
     1201 attgacgata acgatgtagc acatcgtcaa aaaggtcgtc aaacccgcca tcaactcggt
     1261 acgtaccgtc gtaccgtttg ccttcaggtt aaaaacccta tccagcaatg tttgttttga
     1321 aatatccata tctagcagtc cgtttcaagt aaaaaatctg aacccgcaca ccgcaaaccg
     1381 cacaggattc ggcatattga aaaatatacc tcccaaatcg ggaaaaccgg ttcgggaggc
     1441 aataagttta cggcagacgg cacattattt gtctgaattg tgttgacgac gcaaaattgc
     1501 aggaatttca aaatcgtcaa ggacagactg gttatcgaaa tccgctgctg tcagattcat
     1561 ggtgcggata ccgcgattgg tgcggatcat accttcgaca ttgtggcttt gctcctgttt
     1621 ggacggggca accgcttcta cttcccttgc cggagtagga tcgaccgctc ctttttcttt
     1681 cagaccggta gcgataatgg taatccggat ggcatcttcg ctcatggtct cgtcttcagc
     1741 ggcaccgaat ttgcattcca aatcgggatg cgcgctttgg ttgacgattt tcatgacttc
     1801 agacaactcg gacattttca ggcaacccgg agcagtcgta atattgacca gcacaccgcg
     1861 tgcaccatcc aaagttacat cgtccagcag cggactggaa atggcctggt cggtcgccat
     1921 acgcgcacgg tcgatacctt gggcataacc cgaacccatc atagcgatac cgcggttgct
     1981 catcacggtt ttcacgtcgg caaagtcgag gttgatgatt tcgctcgggc aagttaccac
     2041 ttcggaaatg cctgcgaccg catcgcgcaa tacattgtcc gccgcgcgga aggcttcgcg
     2101 catagttacg tcttcaccca atgcggtcat cagtttgtcg ttcgggatga taatcagcga
     2161 atcgacgtgt tctttcaact gttccaaccc tgcctgtgcc acatggacgc gtttgccttc
     2221 ataagaaaac ggacgggtaa ctacggcaac ggtcagaatg cccaaagact tggcaatctc
     2281 agcaacaacc ggcgcggaac cggtaccggt accgccgccc ataccggtcg tgataaacag
     2341 catattggca ccgcgaatgg cttcttcaat ggcctcccga tcttcctgtg ctgccgcacg
     2401 gccgatatcg ggattcgcac ccgcgcccaa accgcgtgtc agattcgtac ccaactggat
     2461 tctcttcgcc gcatggtttt ttgccaaaga ctgcgcatct gtattggcac tgataaactc
     2521 cacactgcgc acattgttgg caaccatgtt attgattgca ttgcaaccgc cgccgcccaa
     2581 gccgattact ttaattaccg cagggctgac tgccgattct gccacgtcgt aaacaaattc
     2641 cattcaaaaa ctcctgatcg ccccgttcag aggacggttt aaataaatta ttatttatta
     2701 tataagaagt atcttactgc tggcaaaata cttctcacct gtcaaacggc aatccaactg
     2761 ttcagaggtt gttttcaatc caccgcttca atcttgccaa caaaccgccg cccccttctc
     2821 tctcttgcac tgtaccgttt tccggttgcg gcaagtttcc ttccagcttg catgctgcat
     2881 gaagcagccc gatagcggta gaaaagcgcg gtgtgcggac acggtcggac aaaccgccca
     2941 tttcttgggg cgcaccggtg cgtacaggca aatcgaagat tttttcggca aattccacaa
     3001 tcccggtcat catggacaca ccgccggtca gaacgatacc cgcattcagc acttctttgg
     3061 ggaaacccga tttttgcagt tcgcccagca ctacgccaaa aatctcctga atacgtgcgc
     3121 tgatgattgc tgccaaaacc ttactggaaa cctgacgcga tgtccggtca cccacgcccg
     3181 gaacttcaat catctcgccc aagccttccg tatcgcatga tgccacgcca taatggattt
     3241 taatgtactc ggcggcatcg agaggtgttc tcaacgattt ggacaaatct ttggtaatca
     3301 gattaccacc ggccggaatg acggacgtat ggcggatggc gccgttcata taaacggcaa
     3361 tatcggtcgt tccgccacca atgtcaatga cgcatacgcc gaggtctttt tcatcttcag
     3421 tcagcaccgc ctgcccgctt gccaacggtt gaagcatgat ctgatcgctt ttcaagccgc
     3481 acagctcgat acatttttgg acattctgca ctgccgtact tgcaccggta atgatgtgca
     3541 cccgcgtatc cagacgcaca ccgctcatac cgatgggctc ccttacacca agttgggtgt
     3601 cgataatgta gtcttgaacc acggcatcga gaattttttg atcgggcggg atattgattg
     3661 cctttgccgt ttcaatggcg cggtcgatgt ctgcctgtgt tacttctccg tctttgattt
     3721 taaccacacc ttgcgaatta aggctgcgga tgtggttgcc tgcgatacct gtggtaacgt
     3781 gggtaatttt ggtatccgcc atcagctcgg catcattgac cgcctgcctg atggcttgga
     3841 cggtggcatc gatattggtt accatgcccg cgcgcaagcc ccgtgaagga gcctgcccca
     3901 aaccgatgat gttgattttg tcgtcatctt gaacttcccc gatcagtgcg aggactttag
     3961 acgtaccgat atccagtacg ctgatgtatc ttttctgctg ttccattgtt cgtctgccct
     4021 taaaactgat tgaaatttgc gtcgcaccgt ttcagacggc acggccgtaa tatgtccgat
     4081 acctgttccc actattcttc ggattctttt tcgggtaaac cgtcgggagc atggcggact
     4141 gaaaatccgt ccttatacct catatccaca taggataacc gatttttatt cttacgcaac
     4201 agatgctgcc acgcttcggt aaaaagccgg aggcgtttca tctcgttttc ccgtccgagc
     4261 ctgacggtga tgccgttgtc caaaacgaca ttccacgccg aacgtgccgt ataggtcatc
     4321 tctttgatgc ccaaaccctg ttttgccaaa acagtcgaaa attcgtcata acggcggagc
     4381 atttcggcag acgttccttc cgcgcctctg aataccggca ttccgggtct gtccaagcgg
     4441 gcttcaaaaa cattgccttc gccgtccacc aaggcatggt cgccccaacg tgcaaccggc
     4501 ttgcgctcgg tcaggacgac ctcaaccgta tcgggaaaac ggcggcgcac catgaccgac
     4561 gcaatccacg gataccggcg gtaggcttcc tgtgcgccat tgatgtccgt cctcaaaata
     4621 ttcccatgga tgtattcttt cgccaaactg cccaatgcct tcttatcgga ataaaccagg
     4681 ttgcccttca gcgacacctg cttgacgggc agatgattcg aattgtaaaa ccaaaccagc
     4741 ccggacgcag caagcagcat cgccatcatg acaagcagcc agcgcgtcag ccgttccatc
     4801 gcttcggcat tatcccacat gtgcggcctt caaaatttca atacataaat cggcaaaacc
     4861 cacgcccgta acggcagcgg atttcggtac taaactatgg ccggtcatac cgggcagggt
     4921 gttgatttcc aacagataga gtttgccgtc ggtatctttg aggaaatcga cgcgcacgca
     4981 gccttccgca ccgattgcct gtgcgccgcg aaccgccagt tcgcgcatca ggctttcttc
     5041 ggcttcggtc aaatcttccg aaggacattg ataaatggtg tcgtctcggt tgtacttggc
     5101 ttcgtagtcg taaaactcgg ttgcggggat gatgtgtatg ccgggcagcc ctttgccgtt
     5161 caggacgggg caggaatatt cgccgccgcc gataaaacgt tcggcaatga tttcgccctg
     5221 aaggtgtttc aattcttcgt aaacgctttt cagacggcct ttttctttga cttttaccac
     5281 gccgacgctg ctgccttcgg ccgccggctt cacaaacatc ggcagaccca atttttcttc
     5341 gacggcatcg aaatcggtat catcgtacag tacggcgaac tcgggaacgg gtaatcccaa
     5401 tgcctgccaa atcagtttgc agcggtattt gtccatgccg atggcggagg cggcgacacc
     5461 gctgccggta tagggaatgc ccaacagttc caatgcaccc tgaacagccc cgtcttcgcc
     5521 gtaagtaccg tgaaggatgt tgaatgccgt ctgaaagccc cgctccttca gttcggataa
     5581 cggcgtttcc ttagggtcga aggcgtatgc gtctatgcct ttgcttttta aggcgttcaa
     5641 aatagcggta ccgctgtcca gcgagatttc tcgttcgctg gaaaaaccac ccatcaatac
     5701 ggccactttg ccaaaattct gcattgtttt tgttctttcc taattgcttt atgcttgttg
     5761 tcagaggtcg tctgaaacct gattgcggtt tcagacgacc tttatatgat gttccgtctg
     5821 tcaggcgggt gtatctcaaa tctgtttcga caattccaac agcgcggagg gaacgcggtt
     5881 gatgcttccc gcacccatat tcaacacaac atcgccatcc tgtaaaacat tcatcagcat
     5941 ttgcggcagg tcggcgacgt tttcgcagta aatcggctca agtttgccca atacgcggat
     6001 agcacgcgcc aaggcgcggg agtcggcggc ggcaaccggc tcttcgccgg cggcataaac
     6061 ttcggtcagt accagcgcat caacggtatt gagtactttg gtaaagtctt caaacaaatc
     6121 gcgcgtgcgg gtatagcggt gcggctggaa ggcgagtacc aaacgttttt ccggatacgc
     6181 gccgcgtgca gcggcaaggg ttgccgccat ttcgacgggg tggtgtccgt aatcgtccac
     6241 cagcaaagcg gtcccgccgt ttggcaactt gatgtcgccg tatttttgga agcggcggcc
     6301 gacgccttca aagccgagca agcctttttg gatcgcttca accgatgcgc cgacttccag
     6361 cgccacgccg atggctgcca atgcgttcag cacgttgtgt ctgccgggca tattcagcac
     6421 gacttcaaac gacccctgct catgtccttt catttgaaca tggacggtga atttcatttg
     6481 cgcgccgacg ttttcgatgt cggtggcgta gatgtcggcg gtatcgtcca aaccgtaagt
     6541 agcataaggt ttgctcactt tgggcaaaat cgcgcggacg tgttcgctgt caacacacaa
     6601 aaaggctttg ccgtagaagg gcatacggtg gatgaaatcg ataaacgcct gatgcagttt
     6661 ttcgacgctg tgcccgtagg tatccatatg gtcttcgtcg atattggtaa cgacggacat
     6721 aatcggggtc agatgtagga aagaggcatc ggattcgtcg gcttcggcaa cgatgtattc
     6781 gcctttgccc aagcgggcgt tggtgcctgc ggcgttgagt ttgccgccga taacgaaagt
     6841 ggggtcgagt cctgccgcgc cgaggatgga ggcggtcagg ctggtggtcg tggttttgcc
     6901 gtgcgtaccg gcaatggcga tgccgtcacg gaagcgcatc agctctgcca gcatcaaggc
     6961 gcgcggaata acgggaattt gccgctccaa cgcagcgaca acttcgggat tttctttctt
     7021 gacggcggta gaggcaacga cgacatccgc accgttaacg tgttctgcgg tatggccggg
     7081 ataaacttga atgcccaggc tgctcaaatg ctcggtagcg gcatttcgcg cctgatccga
     7141 accggaaact ttaaagccca aattgtgcaa gacttcggcg ataccgctca tgccgacgcc
     7201 gccgataccg acaaaatgga tgttgcttac tcgatttttc atcataatgt tgcgttccgg
     7261 ttggttttta gtgcgtaaag gcgttatttt aaaggtctga ccgtttgcgc accatagttt
     7321 tctgacaaat atatagcgga ttgaaataaa aaacatccat gccgtctgaa cggcttttca
     7381 gacggcatgg ttcggcagtt tacgccgcac acgcaatcgc ggcttcggca acgtcatccg
     7441 cgctgtgcgg caatgccaac gtacgggcgt tttccgccca tttgaggcat ttttcgcggt
     7501 tgaggctgcc gaggatttcg gcgagttttt ccgccgtcaa ctgggtttgc ggcaacagca
     7561 gccccgcttc tgcctgcacc atgaaacgcg cgttggcggt ttgatggtca tcaacggcgt
     7621 gaggatacgg cactaacaac gcgcccagcc ccgccgccgt caactcggca atcgtcagcg
     7681 cgccggcacg gcaaatcacc aaatcggcat cacggtaggc ggacaccatg tcggtaataa
     7741 attccacgca ttccgctttc acgcccaacg cgtcataatc cgcctgaaga ttgcccagct
     7801 tgttacgccc cgactggtgg tacatctgcg ggcgcacctc ttcaggcagc agtgccaacg
     7861 cctgcggtac ggttttgttc aaaacgtccg cacccaaact gccgccgacc accaaaattt
     7921 tcaggcggcc ttcgcgccct tggaagcgtt cggcaggcac gggcaggttg ctaatatcgg
     7981 cgcggacggg gttgccgacc aaaccgcctt cgtggctgaa cgctttcgga aaagcgtaca
     8041 acacccgttt cgcccagcgc gacaggtggc ggttggacaa gcctgccacg gcgttttgct
     8101 cgtgaatcac aatcggcacg cccaagagtt tcgccgccag accgccggga aaggtaacaa
     8161 aaccgccgaa gccgatgacg cactcgacac ggtgtttgcg gataatccgc tgcgcttcgc
     8221 ggacggtttt gtacagagta aacggaagca tcagcttgcg tttgatgccg ttgccgcgta
     8281 ttcctttaat cgccagcgtt tccaagcgta tgccgtattg cggcacgatg cgctcttcca
     8341 tcgaatcctt gctgcccagc caaattacat gatgaccgcg cacgcgcaat gaatccgcca
     8401 cagccagagc tgggaaaatg tgtccgcccg ttccgcccgc catcagcata aaggttttac
     8461 cgcccatgat ttactccacc cggtaaccgc gcattttccg gcggttttca taatcgatac
     8521 gcaacagcag catcatgctg atcagcatga aaaagactga cgaaccgcca taggacatca
     8581 acggcagcgt cagacctttg gtcggcaaag caccgatgtt cacaccgata ttgaagaaac
     8641 tttggatacc gatccaaatg ccgatacccg aagcgatata ggcgttgaaa gtcaaaccca
     8701 aatcgcgcga ctgcttgccg atggaaaacg cccgcaccac cagccagccg taacagaata
     8761 tcagcacgca catcccgaag aagccgaatt cttcagcgat gatggcaaaa ataaaatcgg
     8821 tatgcgcttc cggcagaaag ccgcgtttgc tcaaactcgc acccaaaccc ataccgaacc
     8881 actctccgcg cccgattgcc atcagagagt gggtaagctg gtagccggca ccctgcgggt
     8941 ctttccacgg gtccaaaaat gccactaccc gctgcacacg gtagggagcg gcggtaatca
     9001 tcagcaccat cccacccaag acgctgccta ccaggacaaa aaaatatttc cacggcaatc
     9061 ctgccagaaa cagcattcca acggtaatga cggtaatgac gacaaacgaa ccgaaatccg
     9121 gctgtaccat tatcagcacc aaaccgaacg ccaccagcat aatcggcagg atgatcgccc
     9181 ggaaacggcc gtacatttct aatgtttcac gacgtgcctg cggattggtg gcggacataa
     9241 tcaggttggc cgtcccccgc caaatcgact gccaacccaa actttccatg ctgcgcaaca
     9301 cttcttcacg gcgcgtgaac aggcttgcca aataaaggat gactgccagc ttgaacagct
     9361 cggtcggctg gaaattcaac ggacccaaag gtatccaacg ggtcgcgcca ttgatttcgc
     9421 gcccggcaat caatacggct accagcaaca ggccggataa ggcaaaaatc cacggcacaa
     9481 gccgccgcca tgtcctcatc ctgcaaagaa accataaaaa accgctcgct ataaggccgg
     9541 caacgacgaa ccccgcctgc ctggtcaaat agaaaaactg atcgccgcct tccttcgatg
     9601 ccaaatacac agaagccgaa taaatcatca gcaggccgaa cgccgtcatc agcaccacca
     9661 tccacaaaag cggcgcgtcg aatttcctgc cgtcgcgcac aatcggcctg tcgagcagca
     9721 gagtgtggac accgtcgccc acttttacca atacttccga aatcttcaaa aaaccacctg
     9781 ccagtctgtt tgcacctgcc gcaaagggca aaaatttcag acggcagaca atgccgtccg
     9841 aacatacgat acatcccaaa tcggtattct aaatctttac ttgccgccca acaatgacgg
     9901 cgtttgtgtt tcggacggca tcacaaagcc ttaaacgctt cgataaacac ttccgaacgg
     9961 tgcgcgtagc ctttaaacat atcgaaactc gcgcaggcgg ggctgagcaa gacaatatcg
    10021 cccgcttcgg cttgggcgta tgccgtctga accgcctctt ccaaagtgac gcagtcggtc
    10081 aggttcaagc cgcagccgtc caaatcgcgg cggatttgcg gcgcatcgac gccgatcagg
    10141 aacacgcctt ttgccttatc tttcaacgcg tcgcgcaggg gcgtgaagtc ctgccctttg
    10201 cccatgccgc ccaaaatcac gaagagcggg ttttgcaaac cggcaatcgc ggcggcggtc
    10261 gcgccgacat tcgtgccttt gctgtcgtcg atgaatacca cgccgttttt ctcgccgatt
    10321 ttttccacac ggtgcggcaa gccttggaag gttttgacgt gttccagcaa tgcttcgcgc
    10381 ggcaaaccga cggcctcgca caaagccacg gcagccataa cgttggcggc gttgtgcaaa
    10441 ccttgcagcg ggatgtcttg cgtagaaatc aaatcttcat tgccttgttt taaacagccc
    10501 gtcccgcgtt ccaaccaaaa atcggcttcg tgttccaagg aaaaccgttt cacttcacgc
    10561 cctgcccgtt tcatggcgcg gcagaacacg tcgtccgcat tcaaaacctg cactccatcg
    10621 ccacggaaaa tcttggcttt ggtatgcgcg tagtcgagca aatcgtcgta gcggtcgaga
    10681 tggtcttcgg aaatgttcat caccgtcgcc gcagtcgggc gcaggctttc ggtgttttcc
    10741 agttggaagc tggaaagctc caacacccac acgtccgcct ttttgccttc gcgctgcaat
    10801 tctgcctcca aaaccggcgt accgatattg cccgcaatga cggtatccag cccgcacttg
    10861 atgcagagat agccgaccag gctcgttacc gtggttttgc cgttgctgcc ggtaatcgca
    10921 atcaccttgt cgccgcggcg gttcacaatg tccgccagca attcgatgtc gcctagcacg
    10981 cgcccgccgt tttgcttgaa cgcctcgata tccggctgcc gctcgctgat gccgggactg
    11041 agcgccagaa tatcgaaacc gttgtccaac gcatctttca gacgacccgt gtaaaacacc
    11101 agcccgtcaa acatcttacc gatttgcgcc acgcgctcgg ctttcagctc cgcatcataa
    11161 gcggcaacct ccgcgccgtt tttgcgcaga taggcaatca tggaaatacc cgtgccgcca
    11221 agtccggcga cgagggtttt tttgttttgg aaagtcattt tggttttgtc ctaaaacaaa
    11281 tcatattggg caggagacgt ccgcccttgc ccaagccgct ttcagacggc atcgcgagcc
    11341 gattaataac ccgccttcag gcgttggtca ttgtcgcagc tgttttggtc tccgttttga
    11401 caagccttgc caagccattg ttgagcgagc gcaaggtctt ggcgcacgcc gcgtccatcg
    11461 taatacatca agcccaaatt gtattgggct tgggcatccc cttgttctga agccttgcga
    11521 taccactgca ctgcctgtac ataatcttga cgaactcctt gtccattttc atacatcacg
    11581 cccaaattga attgggctgc tgcatttccc tgttctgccg cctgcaagtt ttcccgaaaa
    11641 tccgatacgt cacccgccca caccgcttgg ttcaagccca aggcaatcag ggcggcggca
    11701 agccatttga ctgtctgttt catggttttc tcctgtttta gtataaggcg ggtttcagcc
    11761 gccgttaacg ataggacggg gcggattgcc gccgcaggtt tattgcgcgt tcaaatgccg
    11821 tctgaaaggt gttcggacgg cataggttca gcgaattttg agggtactca aaccgatcaa
    11881 taccaagacg atggtaataa tccagaaacg gacgacgact tgggtttctt tccagccttt
    11941 ttgttcgtaa tggtgatgaa tcggcgccat caggaagatg cgttttttgg ttttcttgta
    12001 ccagccgacc tgaagcataa cggacacggc ttctacgacg aacagaccgc ccataatgac
    12061 gaggacaaat tcttggcgga cgatgacggc aacggtaccg agcgcggcac ccaatgccag
    12121 cgcgccgaca tcgcccataa agacttgcgc gggataggcg ttgaaccaca aaaatccgag
    12181 gcacgcgccg cacatggcgg tgcagaatat agcgacttcg ttcgcgccgg cgacataggg
    12241 aagctggagg tattgggaaa attggtagtg tccgctgacg taggcgaaaa tggcgagccc
    12301 ggcggcaacg aggacgacgg ggaaggcggc aaggccgtcc aagccgtcgg tgaggttgac
    12361 ggcgttggat gtgccgacga tggtcaggta agacaacacc aaaaagccga ccacgcccag
    12421 cggcagggcg atttgtttga aaaacgggac aatcaaaata ttgttggcgg aattggcggc
    12481 aaggtaaaac aatgccaaac cggcgataac ggcaacgctt gactgccaca ccattttgaa
    12541 tttggcggac acgccgttgg ggtctttata aacgactttg cgccagtcgt cgtaaaaacc
    12601 gagcgcaccg gtggcaagca gtacgcccaa gagaatccag atatacgggt tcgcccagtt
    12661 gccccacaac agggtggaca cggtaatggc ggtcagaatc agcgaaccgc ccatcgtcgg
    12721 cgtgccgttt ttgacgaggt gggtttgcgg gccgtcggta cgcactgcct gcccgcattt
    12781 gagcgcggtc agcctgcgta tcgtccacgg gccgaacatc agggaaaacg ccaaggcggt
    12841 caacgccgcc ataacggcgc ggaatgtggt gtattgaaaa atattcagac cggttaacca
    12901 gttgctgaaa tgtgcgagcc ataaaaacat ggggcttcct tttttgtttt gtcgttatag
    12961 cggattaaca aaaaccggta cgtcgttgcc ccgccccggc tcaaagggaa cggttcccta
    13021 aggcgcccaa gcaccgggcg aaccggttcc gtactatccg tactgtctgc ggcccgccgc
    13081 cttgtcctga tttttgttaa tccgctatac atcgggcttg cgcccgcttg aggtttgctt
    13141 gctgtccggc aaggtttcgg acggcttttg cagattaacg tgcagggact ttcccaatca
    13201 cgcaaatcgg gtagctcccc ggatttttgc cgccgcccaa gtctaaacat aaatcttcat
    13261 aaagatacgc ctgcgtccgc attccggcat aaaacgcgcc gaccagcgcg gcaatcagca
    13321 ataaggcttt aaaaaaacgt cggcttttca ttttcactta tcctccaatg cctcgaccac
    13381 ttcttccatc tgcataaagc gcgaaccttt caccaacacg gtggcgcgtt cgggcaaatc
    13441 gtggctcaac acttgaatca acgggtcttt ggcggcgaac cacaaaccgt ccgcgccaaa
    13501 tttttccgcc gcttcgacgc tgttgtcgcc gacaaaataa gccgcttcga tgccttggtc
    13561 gcgggcgtac gcgccgactt cggcgtgcat ggcggcggct tcgtcctcgc ccagttcgcc
    13621 catatcgccc atcacgaaaa tgcgcggcgc aggcatacgc gccaacacgt caaccgcggc
    13681 tttcatactg tcgggattcg cattataagt atcgtcaatc agggttgcgc ccttgatgcc
    13741 ggctttgacg ttcagacggc ctttgatgtt gctgaagcct tgcaaacctt ccgccacatc
    13801 gttcaaactc aaaccggcag ccaaagccag cgcggcggca gcggcggcgt tgtggacatt
    13861 gtggcggccg ggaacaggca gcaccacggc agtgcgctcg tcgccgcaca ccaaatcaaa
    13921 ttcgcacgac aaaggtttca gcacgatatt ttccgcgcgg acatcgccgc tatcgacgcc
    13981 gaaagtgcac gtattcaaat taaacgttgc cgttttgaag acagccatat tggcatcttc
    14041 ttgaggaatc agtgccatgc cgtctgaaca taagcctgca taaatctcgc ttttcgcttt
    14101 ggcaatatcg cccactccgt cgaaaccgca tccgacatgg gcgcgcaggg cgttgttgac
    14161 caaagcggca tcgggtttgg cgatttgcgt taaaaccgcc agttcgccaa aatggttcat
    14221 gcccatttca atcacggcat agcggtgttt ttcgtttaat ttcaataaag tcagcggcaa
    14281 tccgatgtgg ttgttgaagt tgcctgccgt cgccgaaacg gcatcatcgc cgaaacggcg
    14341 gcgcaatacc gcagccagca tctccttcac cgtcgtcttg ccgcccgaac cggtaatgcc
    14401 gaacacaaac gggttcacat tatcgcgcca cgccttcgcc aacgtttgca acgcggcaag
    14461 cgtgtcatcg actttcaacg cgccgcccaa agccgcgcaa tcttcgcgcg aaaccacaac
    14521 cgccgccgcg cccgcagaca atacgcctcc aacaaaatca tgcgcgtcaa accgcccgcc
    14581 cgccaatgcg aaaaacacat cgccttcccg aatatcgcgg ctgtcggtta cgatgcgcga
    14641 cacgggtttg ttttcagacg gcatcggaag cttgagggct tggcagatga aatttaggtc
    14701 cagtggtttc atatttgctt tcgttaatat tcgggcggcg gacacatcgg tagcggctga
    14761 tttttttatc gcctgttttg ctgtggtaaa acacagatta ttttcccatt ctcattcggc
    14821 attttttctg tacgtatcat tttttagacg tatttttagc cgatttgcct tttcccgcat
    14881 accacggcgc ggggtcgtcg gactgtctgt cgataaaggc aaggttattg ccttcgcccg
    14941 gcacatcggg gacattcccc caaaaatcat agccgtcatc gggcaactcg tcggtttcga
    15001 tacccgtcca actgccgaat ccgcgtaaaa aattaaacgc gctgcgcctc actttaatca
    15061 tttcgcgccg cgtgtcatcg gacgaaattt taaccagcgg cacgccgtag ctttgacgct
    15121 tccactcgcc gtgtgtcaac gttcgctcgc cgcctttgcc gacatgcatc aaaccgtggt
    15181 cggaaaagta aaccaaagac cagctttctt tattttcatt caatatctta accgtatctt
    15241 ctaaaaattt atcggtttgc gcgatggtgg aaacatagca ggatattttt tcagtttgat
    15301 actgaaaccg ccgcgcatcc ttatccaaac gtgtgcaaaa atcactgtgc gaacccatca
    15361 ggtgcatcac aatcagccga ggcttcgtgc ctgcatttcc tgtcaaaacc cgtttgaacg
    15421 ccggcaacaa aaggcggtcg ctcaaccccg cgcttttgcc ataatcaccc ctttgggtaa
    15481 accacggata atcgctgcgt agggcatagg tggaaatttc gttggcaaaa tgccccaaca
    15541 ttccttgatt agacagccac gccgtccgaa aacccgcctg cttcgccaag ctgacaatgt
    15601 tattgttcgg ctctcccggc agccccaaag tctgcggcag cgaaagattc gtcgcgtggg
    15661 cggtcgattg gtaaccgttt atcaacagcc ctttggtccg actcaaaaaa ggcgtatcgg
    15721 gcaatgggaa accgtaaaca ttcatataat ccgaacgcgc gctctcaccg ataaccacaa
    15781 cataattttt atacttgggc gcaacatgac ggatatgcca tgttgacgct ttttttgctg
    15841 cttccaaaat gtgggcgcgc ttggcggcat attgtgccgg cgcggaagcc aaatcgtaat
    15901 acaggtcgaa aatattcaac aacaggccgg catcaggttc tcgccaatct ttatcgccgg
    15961 cgattttctc catcaccgcg caggataaaa tcaaagtcaa taatatggtc agccatattt
    16021 tgctgcggcg tttatagttt tttacgtcag caaatacccc cacagataca caatatttcc
    16081 aaactgtcaa gccaaaaatg aaaaataatg cctgcacaaa ataaagcgac ccgggaagat
    16141 tgccgacaaa ttcacgcgcc tcggcaggat tgctttccaa tatcgaaccg actatctgat
    16201 aagaaggcgc accatacagc cagccgaccg gcaaatatag ggcggttgtg ccgacgtaaa
    16261 tcagtaaaac aactgaagaa acgcgcggga agccgcgtgc caataataat aaaaatacaa
    16321 ccgaagccaa agcgcccacg gcagaataac ggtagccgta ttcatattcc aagtgataac
    16381 ctgatgcgat ggccgcgcca aaacaaaagg ctaaaagcgc gcaaatgccc aatgtatttt
    16441 ttaatgtctg atccatattt tttattcggg cggattgttt catacgatgc cgtccgaata
    16501 tcccattatt tacgagttaa caaagcctgc ccgacgattt caagatcgga aaaacggtgc
    16561 ttcacgccct gtacatcctg atagttttcg tgtcctttgc cggcaatcag gatgatgtcg
    16621 tttgcggcgg cctgctcaac cgcataacgg atggcggcgg cacggtcggc ttcgacgcat
    16681 tcgggcgcgg gcacggcggg caggatgtcg ttgatgatgt cttgcggatt ttccaaacgc
    16741 gggttgtcgc tggttacgac gactttatcc gcgccctgta cggctgccgc gcccatcaat
    16801 gggcgtttgc cgcaatcgcg gttgccgccg caaccgaata cgcaccataa ggccgcaccc
    16861 tgcggtttga tttcctgcaa agtggagagt gctttttcca atgcgtcggg cgtgtgggcg
    16921 taatcgacga cgaccaaggg cttgccgctg ttcatgatgc agtccatgcg ccctgaagcg
    16981 ggacggattt ttgccagcac atccaatacc ttatcaagcg gataaccgtt ggcacaaagc
    17041 aaggcgatgc aggcggcgag gttttgcgcg ttgaaccgtc cgagcaggcg ggtgcggcat
    17101 tttccttcgc cccacggggt tcggaatacg gcttctatac cgtctgaaga ggcggtgaaa
    17161 tcggtaatgc ggatgtcggc gtgttcgctg aaaccatagc tgtaaacggc taaatcggga
    17221 cagtcttttt tcagacgacc tgcgagttcc gcgccgtatc cgtcatcggt attgataacg
    17281 gcgtgtttca agccgtgcca gtaaaacagg cgcgacttga tagcaccgta ggcttccatc
    17341 gtgccgtggt agtcgaggtg gtctcgggtg aggttggtga agattgcgct gcggaacggc
    17401 acgccgttga ctcgcgactg gtcaaggccg tggctggaaa cttccatcgc ggcggctgtt
    17461 gcgccttgtt gacggaaacg gtagagcagg gtttggacat cgacgggggc gggggtggtg
    17521 tgcgcggttt cttccaatgc accccaaaag ccgttgccga ccgtgccgat aatggcggtt
    17581 ttttcgccca acaaatcggc ggcttgcgcc agccattgcg tgatggaggt tttgccgttg
    17641 gttccggtta cgccccagac tttgagaccg tctgaaacgt tgccgtaaac ctgtgccgcc
    17701 aatatgccgg cacggtgttt caaatctttg atgccttgat tggggacttt ccattcggga
    17761 ttccacgcga atctgccgtc gtcgtcccaa aaaacaaaag ccgcgccgtt ggcaacggcg
    17821 gcggggatat aactgcggcc gtcggtatat tcgccctgac aggcaacgaa aatatcgcct
    17881 tgtttgattt ggcggctgtc tgaatgcaac aaacgccctg ccgcgtttgc acacgacagg
    17941 gtcgggatgc cggtttcagc caaagggctt aacttgctga acataaaaca atctcgttga
    18001 tactcggatt aagacggtgt tttgacggct gcggcggtca gtggcttggt cggggaaatg
    18061 cccaagatgt tcaggctgcc gcccataatt tttttgaagg gcggccctgc cactacgccg
    18121 ccgtaatagc cgtgggcagt cggttcgtcg atggtaacgg ccacaatcac acgggggttt
    18181 ttggcggggg caaaaccgat aaacgtaccg acgtgtttgt tgtccacata gcggccattg
    18241 accagtttgc gcgccgtacc ggttttagcg ccgacatcga aaccgtccac cgcacccgcc
    18301 gtaccggtgc cgcccggctc ggttacggaa accatcagat tgcgtacctc gcgcgcggtc
    18361 gattctttga atatgcgttt gccttgcggc gcaaccgcct gcttctcaaa gctgagcggc
    18421 agcaaaacgc cgtcgtgcgt cagtgcggta taggcgcgcg ccaattgcag caggctcaat
    18481 tgcaggccgt aaccgaaaga catcgtcgcc tgttcgatgg gccgccacct gcgccaattt
    18541 ctcaacaaac ctgcagtttc ccccggaaag cccgagtgca tacgcacacc gatgcccaat
    18601 tcatgataga agtcatacat ttcttcggcg ccgaaacgcg cagacagttt gcttgtgccg
    18661 acgttggacg atttctgcat aatgccgcgc acatccaaag aggggtaaac atgggtatcg
    18721 tcgcgcacgg gagacggtcc gattttataa ggctgcgtat tcagccgttc gttcaaatcg
    18781 gttttgcccg catccaatgc cttcgcaatc acgaacggtt tgattgccga accaggttcg
    18841 atcatatcgg ttacggcacg gttgcgccgc tgttcgctgt ctgcccggcc gggtctgttg
    18901 ggatcgtagg cgggcgtatt ggccaaggcg aggatttccc ccgtgcgggc atccaaaacc
    18961 accaccgttc cggcttttgc ctgatggtat tcgaccgcct tgttcaactc ttcataggcc
    19021 aaggtctgaa tcctctgatc gagggaaagg atgatgtctt tgccgttttg cggtgcttta
    19081 ttgcgcgggg agtccaagct gtccacaata ttgccctgcc ggtcccgcaa aacaacttcc
    19141 gcgccgtctt cgccatacag gctgtcttca agcgaaagtt ccaaaccttc ctgacctttg
    19201 ccgtcaatat cggtaaatcc gatgacgtgt gcaaacaggt tgcccatcgg gtaatggcgt
    19261 tttaattctt tttcaaatac aaagttttcc aaacccaagg ctttgacctc ttcggcaacc
    19321 ttgggatcga gctgccgctt gatccaaata aacgacttgc ctttctgttc gagtttgttc
    19381 ctcaaaacat cgaccggcac atcgacaagc tcggacaggc gttccaattg ggcggcagac
    19441 ggcatttcct tcatatcttt aggcacggca aacagggact ccgtcggcgc gctcaacgcc
    19501 aaaaccgcac cgttccggtc cgaaaccgta ccgcgtgtag ccggcaatgc ttgagtccgc
    19561 acaatccggt tgtcgccctg ttctttcaaa aagttatacg ttaccgtctg cagatacagc
    19621 ccgcgggcaa tcagacaggc aaacaagacc gccattgcca tcaggacgaa gctaatccgt
    19681 ccgttactgg tcatcggctt tttgacctgc tcttctttgg gcagcatccg gggcttatat
    19741 tcgcttttaa tcaacatttt tacttctcgt tattattatc ctgatgcagg aatccgattc
    19801 cggcacacag gctgcttcta tctttgatgt tccaccataa aggtattgcc cgaaaccggc
    19861 ggatggaggt tttgtttttc tgccgccgcc ctgatcgctt cgtgattcgc caaacgcgcc
    19921 tgttgcaccc tcatttgcgc ataatcctgc tccaaggcga tttcctgttt tttcgccttg
    19981 tccaaagctg tgaagttgag cctgtactgg ttttgctgca tcacaacgga aaaagcggaa
    20041 acgcacaccg caagcagcag aaagaaattc gatttgttca ttgccgttca gacgtttttc
    20101 tctgttatta ttccggtatc ggaccggcag tccgctccgc cacacgcaaa actgcgctcc
    20161 tcgccctcgg gttggcggca atttccgctt cacccggctt taatgccctg cccacgattt
    20221 tcaggggcgg ctcgggcaaa tccgcttctc tgaccgccgc ccagctcggc aggggctcgt
    20281 gttgcgaata ttttttgaca aactgcttca caatgcggtc ttccaacgaa tggaaagcaa
    20341 tgaccgccaa acgcccgccc tctttcagac ggcacatgac ctgcggcaat accgccccta
    20401 cttcttcaag ctcgcggtta ataaagatgc ggattgcctg gaaggtgcgc gtcgcaggat
    20461 cctgcccccg ctcgcgagta cggacgtttt gtgccacgat ctgcgccagc ttgcgggttg
    20521 tatcgattgg actttccgcc cgttgcgcga caatggcgcg cacaatctgg cggctaaacc
    20581 gctcttcacc ataattcttg attacctcgt gcaaatcctg ttccgacgca accgctatcc
    20641 actctgcggc agacataccg cgcgtcgtat ccatacgcat atccaaaggg gcatcgaaac
    20701 ggaagctgaa accgcggctg ccgtcatcga tttgcggaga cgaaatcccc aaatcaaaca
    20761 gcgcaccgtc caccttgccg atacccaaac cgtccaatgc cgtctgaaac gaagcaaaac
    20821 cgccatgcac gacaccgacc cgtttgtccg aacgcgccag ctcttctgcc acagcaatcg
    20881 cctgcgggtc tttgtcgaaa acaatcaacc gccccgcatc gcccaaacgc gacaaaatca
    20941 gccgggaatg ccctcccctg ccgaacgtac cgtccacata gacaccgtct tcgcgcacgg
    21001 caagcgcatc caccgcctca ttcagcaaga ccgtgatatg ccggtaactt tctgctccac
    21061 tcacaattgc aaatccgtct gactcaactg gaaggcaagt tcgtcaggat cgtcatccaa
    21121 agcctgaacc atctcagcct cccactgctc gcgaccccaa agctccaaac ggttggcacg
    21181 gccgaccaaa acaacttcac ggtcgaaatc cacccttttc ctcagtccgg caggaaccag
    21241 caccctgccg gcgctgtccc attccaaaat ttccgcgtta tgcagcaaaa gattttgaaa
    21301 ccgccgcaaa acagggttat ccgccacttt taagtttaaa agttgcgccg caaccttttc
    21361 ccactccgca acagggtaca tcaacagctt atgtttcgac tcgagcgttg ccactacggc
    21421 aggcgtatag aggcgcgaca gaatgtcgcg gaatttggca ggaacagcca accgcccctt
    21481 actgtcgatg cttaattcgt gtgcgccgcc gaacatgaca tgccccaagc cgaaatcaaa
    21541 atcacaaggg taaaagagac actttgcccc acaattcccc accaatcgac actataagaa
    21601 attttaaaca ctcggtcaaa tcagggcatg aaaactcatt aacatatctg aaatttttat
    21661 tctgtttaaa aacaataaga taaaaaatga cgacaacggc acggcgggtg cagtacagag
    21721 tgaccgaacc aataaacaac tatatatgat taatttaata atataaacac aatatatagt
    21781 attaagataa agccatgaca gcacccgtac caacgtgtaa tatgtcggga aatccaataa
    21841 atttacacaa gctaacattt atcatgctcc tcccctcccc cgaagcacgg caatcctcgc
    21901 tcaatctgca aaccctcatt gccgaagaaa tcggcaaaca cggcaattgg attccatttt
    21961 cacgttttat ggaattggtt ttatacgctc cgcaatacgg ctactacacc ggcggcagcc
    22021 ataaaatcgg caataccggg gattttatta ccgcaccgac cctcacccct ctgtttgcac
    22081 agacactggc acgccaactt caagaactcc tgccccaaac ggcgggcaat atctatgaat
    22141 tcggcgcggg aaccggacag cttgccgccg atttgttggg cagcgtttca gacagcatca
    22201 actgttacta tattattgaa atatcgccgg agctggcagc acgtcagaaa aacctgattc
    22261 aagcccgtgc accggaagca tctcaaaaag ttgtccactt gaccgcactt cccgaagcgt
    22321 ttgacggcat catcatcggc aacgaattac tcgatgccat acctgtcgaa atcgtccgta
    22381 aaaatgaagg aggattactc gagcacatcg gcgtttgcac ggataacggc cgtttcgcct
    22441 attctgcaag acccttgcac gacccgtcgc tttccacctc ggcttccctc tattttcctc
    22501 aaacagatta tccctatacc agcgaactgc acccgcaaca atatgccttt atccgcaccc
    22561 ttgcctcaag gcttgaacgc gggggtatga tattcatcga ctacggtttt gatgcggcgc
    22621 agtattacca cccgcaacgc aatcaaggca cattgatcgg acactaccga caccacgtta
    22681 tccacaatcc tttcgacttc atcgggttgg cagatctgac cgcccacgtc aattttaccg
    22741 acattgccca agccggtacg gatgcaggac tggatttgac aggctatctg ccccagtccc
    22801 attttctgct gaatctgggc ataaccgaac tgttggcaca gacgggtaaa acagattcgg
    22861 cagcctatat ccgtgaagcc gccgctgttc aaaaactgat tgaccagcat gaaatgggcg
    22921 aactgtttaa agtcatcgca ttcggaaaaa atatcggcat cgactgggca ggattccgct
    22981 tcggcgacat ctgccacaaa ctccaaccct cgtgccgtct gaatccgctt cagacggcat
    23041 aaacttttta acatttaaaa acaagaaact aattcaaaat taaaaaatac ggcttgtcaa
    23101 aaaaacaaaa aaacatataa tagctaattc acgaaacggc gaattagctc agtcggttag
    23161 agcagaggaa tcataatcct tgtgtccggg gttcgagtcc ctgattcgct accagatttt
    23221 cgggggtata gctcagttgg tagagcgctt gcatggcatg caagaggtca gcggttcgat
    23281 cccgcttacc tccaccagat aaaaaagcac agaccgtaaa aggtatgtgc ttttttattg
    23341 cctgattgcc ggaaaataaa gaataaacca ctgccttcaa aacaggcaat cgactttaaa
    23401 cctattgccc cgcctgtcct gattttatca gcccgccaga caaacccgac ctgaataatg
    23461 tcttcaggtc gggtttatgg tttcattccc aacttatcca gcccgacaac cacaatatta
    23521 tgatgacaat gccgaaaaca atgcggtaat aggcaaacgg gatatagttt ttcttggaaa
    23581 caaacttcag cagtgcttta accgctacca aaccggaaac aaaagcggca ataaagccta
    23641 tcaaaatcaa accgacatca tgcagggtga aaaatcggta atgtttcagg acatcataag
    23701 ccgttgctgc aaccatcatc ggaacggcca agaaaaatga aaactccgtt gccgttttcc
    23761 gctcgattcc ccaaagcatc ccgcccataa ccgtactgcc cgaacgggac gtacccggaa
    23821 ccagtgcaaa cacttgggca acaccgatca tcaacgcatc aatcggacgc aatgcatcaa
    23881 catcggcaat tttaggctct gctcggcttt ggcgtttctc cacccacaaa ataaaaaaac
    23941 cgcccaaaac cagcatgact gcaacactca aggggttaaa cagatactct ttgatttgtt
    24001 tgtcgaacaa cagccccatc acggcggcag gtataaaagc aatggcaaga ttgaggacga
    24061 aacggttggc tttccggtct tttcccacgc catgcaacac attgctgaaa cgctgccggt
    24121 attcaaacac taccgccaaa accgcaccga gctggatggc aatttcaaaa accttgtgat
    24181 tgctgtgaaa accaatcaga ttgccgaaca caatcaaatg tccggtgctg gaaatcggta
    24241 aaaattcggt aaaaccttct accaagccca tcatcagggc tttcaggaca atcagaaaat
    24301 ccattgcttg cgcttctttc ggatacggga gttcggctat ttctgtacag caggggtctg
    24361 acgcttgcgt tcttccctga ctttggcaac caattcttta atcgtatgaa cgccgccatc
    24421 aaagccatta ttgaagataa cgcggtattt tccgccgaca ataacggtcg gcgtgctgtc
    24481 aataccgtat tgttccgtca gtttctgcat ttttaatgcg acggcggcag cttcggggga
    24541 atcataggcg cgcatcagtt ttttgccgtc aaaacctttt tgagataaag cccattttcc
    24601 ggcaacagcc ctgttttcca aacggatttt ttgttcgtaa actgctttaa acacagcaga
    24661 gtttgcctga tatttcaaac ccgacagctt gaccgcagca gccattcttg ccagaccgag
    24721 catttcaggc cgccagacca cgtgctccgt ccgcagataa gtatcagacg gcaatgcctt
    24781 gcccagtttc aataacaaag gatcgaaatg atggcaatgt acgcaaaaat agccgaaaaa
    24841 ttccaaaacc tcaatttttc ccggctgttc ttgaggaatg ggtttatcca acacaagata
    24901 gtcttcccct tccgtcaggg catatgcctg cgcggacaac actgccgaca gcagcagcgg
    24961 caacagatgt ttaaacttca taattatttg ccttcaatcg cacggatcag gctggcaacc
    25021 ccatgctttt tcaactcgtc ctgcattttt ttcaccgcat cggcagacat attgccgctt
    25081 tgcacgcggt aaagcgtttt atgtcccgcc tgatagccga ccacttcgga agatatgccc
    25141 aagattgcca gtttggcacg ctgcccttcc gcgctccggc ggtcggcata cgcgcccatt
    25201 tgcagataat gcgttgcttc cgccttgcca aacgttttca ttttctgcac ttctttggca
    25261 gcggcactgc gcgctttttc gatgctgccg ctgttgagga tttgttccgg ggtcggtttg
    25321 ggtgcaactt tttccttcgc cgcctttttc tcttcttttg aagctttttt ctctgtttct
    25381 ttagacggtt ttaccgcttg ttttttaacc gtttcggcat ctttcttctg cgctttttcc
    25441 ctgacggttt gttcacgctc ttccgtcagt gctttcttgc gcactgcctg tccgtccggc
    25501 tcttcccgtt ccggctcgcc cgccttttct tcaacctcgt cggctttgtc ggcaacgggc
    25561 tgcttgtcgg cagctttttc cgcatccgac tgctctgcct ctttcgcaac atccggttcg
    25621 gacaaggcgt tttgatcggc cggttcaggt tggatgtctt ccttaggctg gtttttcagt
    25681 ttcaggattt ccgtttctgc aggctgcttc gacggagccg ggattttgaa cgcattttga
    25741 ccgccctggt tcagataaag caaaataccg gcaataatga ccgttgccag tatcaaaccg
    25801 aagaagaaac cggacagacc ttttccggat tgggaaaatt tgttcataaa cataccttaa
    25861 tgtgtttcag acggcattaa cgccgtttgc ttacgggcgg atattctaac aatatcgcca
    25921 tatttgggca aaacctgctt ccattcccgt tcctataaag cacgacggaa gcctatggct
    25981 ttcatgacgt ggcttctgcc gacttcttca tcgcccgcca aatccgccaa tgtacgcgcc
    26041 acgcgcataa tgcggtggaa gctgcgggcg gaaagagaca atttttccag caggctaccc
    26101 aaggcttcct gcgcttcttt ttggatgcag gcttgggaat cgagttcgct gacgctcaaa
    26161 gcggcgttga ctttgccctg ccgtgcgtat tgtttgccgc gggcggcgat gacgcgttcc
    26221 aaaacggacg cactgctttc gcccgcttcc tgctgcatca gttcggcggc ggacaggctg
    26281 gggacttcga tggtcaaatc gatgcggtcg agcagcggac cggaaatttt gcttcggtaa
    26341 cgcgcgacac tttcgggcgt gcagcggcag ggtttgacgg gatgcccgag ataaccgcac
    26401 ggacacgggt tcatggcagc gacaagctgg aatttggcgg gatagacggc ttggcgcgcc
    26461 gcgcgggaaa tgtggatttc gccgttttcc aacggttcgc gcaaaacctc caaaactttg
    26521 cggtcgaact cgggcaattc gtccaaaaat aaaacgccgt gatgcgccaa tgaaatctcg
    26581 cccggacgcg gatccgagcc gccgccgacc atagccgccg cgctggcgcc gtgatgcgga
    26641 ctgcggaaag gacggttgct gtcgagttgt tgttggtggt tgggcaggag cgaacgcaat
    26701 gcccaaactt ctaccaattc gtcttcggcc agcggcggca ggatgccggg cagccgttgg
    26761 gagagcatag acttgcctgt tcccggcggg cccatcatca agaggctgtg tccgcctgcg
    26821 gcggcgattt ccaaagcaag gcgggcagag tgctgacctt tcacatcaca caaatcaggt
    26881 tgtccgccat gttcagacgg catttgcggt acggaacatt cggtttgcgc caaaggttcg
    26941 ataccgttca aatgagcggc gacttcgccc aaagagcgcg cgccgtaaac ggtaatgccg
    27001 cgcatcacgg cggcttgtcc tgcgttttct tcaggcaaaa caaatgcacg ttttgcctgc
    27061 ataccctgcc acgccatcgc caacgcgccg cgcacggggc gcaacaaccc cgacagtgcc
    27121 aattcccccg caaactcgta ttccgccagc ttttcgggtg caacctgccc cgatgcggca
    27181 aggatgccga ttgcaatcgg caaatcgaaa cgccccgact ctttgggcag gtcggcaggg
    27241 gcgaggttga cggtgatttt tttggcgggg aattcaaaac cgctttgaat aatggcggca
    27301 cggacgcggt cgcggctttc ctttacttcc gtatcgggca gtccgacgat gttgaaatgc
    27361 ggcaggccgt tggcaaggtg ggcttccact tcgaccaacg gcgcattcat accgctcaag
    27421 gcgcggctgt aaaccaaggc aagcgacata tttcagacgg ccttattcgc cggcttcggt
    27481 ttgctgcctg atttcggcga cggcttcttc ggcagcggct tcagccgctt ccaatgctgc
    27541 ccgttcggga ttttgcgcgg cttcgagttt cgccagacgt gcttccaaag ccgccagttt
    27601 ggtacgggtt ttaatcaaaa cctgctgctg gatgtcgaat tcttcgcgcg taaccaaatc
    27661 catacggttg aacgcgccgc ccagcatcgc cttaatattt ttttccacat ctttggcagg
    27721 gctgttggcg atggtttcgc tgattttcga gccgacttct tcaaaaagct gcttgccgaa
    27781 cataatctgc atccttcctg aacatatcaa attcaagcgg ctattgtata aggaaaaatg
    27841 ccgtctgaaa acgggcggcg gataatcgtc aaaacatacc gcgccccctt tgcggttgct
    27901 aaaattacac ttcgagacac ctgccgcctg aaaacggatt tcatattccg cgcaacgccc
    27961 gattacatta caagacacta caaaacaata tgctgtttta aatgattttt ccgatgcgca
    28021 tcgtttcaaa ctcggctttt taagccatta agtgctttgc aaacaacaga aattgggtta
    28081 tgctgaaacg gattatttac aatttcatat agtttcatta catatcttat tgtaattgaa
    28141 gataatttat ccgaatcccc tttcgggtat ccggattttc cgttgtactt tcattagaaa
    28201 aacatttcag gcgcaagttg cttgcaattt caaagcctgt aattctgaag ttttagacga
    28261 ggaatagcac atgaatccca tgtacatcac ttttgcaatc tatttggttg cagtccttct
    28321 catcgggctt gccgcttatt tttccacgcg caatttcgat gattatattt tgggcgggcg
    28381 cagcctgggc ccgtttgtta cggcgatgtc ggcaggcgcg tccgatatgt ccggctggct
    28441 tttgatgggt ctgccgggcg cgatttattt gagcggtttg aatgaggctt ggattgccat
    28501 cggcctcttg gtcggcgcgt atttcaactg gctcttggtg gcgggccgcc tgcgcgtgca
    28561 taccgaatat gccaacaatg cgctgacgct gccggattat ttcttccacc gcttcggcgc
    28621 gggcggacac ttgatgaaag tggtttccgc actgattatc ctgtttttct tcacgattta
    28681 ttgcgcctcg ggcattgtgg cgggcgcaac cctgttccaa agcctgtttg aaggtatgac
    28741 ttacaatcag gcaatgtggc tgggcgcggg cgcgaccatc gcctatacct tcttgggcgg
    28801 cttcttggcg gtcagctgga cggatacgct gcaggcttct ttgatgattt tcgccctgat
    28861 tttaacgccg gtgatggtct atctgggctt gggcggcgcg gaacagatgt ctgccgcaat
    28921 tcaaagcgtc gccgcaggca cgggcaaaga atacggcagc ctgtttgccg gtacgaccgt
    28981 catcggcatc atttccaccg ccgcctgggg cttgggctat ttcggacagc cgcacatttt
    29041 ggcgcgcttt atggccgccg aaagcgcgaa atcgctggta tccgcacgcc gcatcggcat
    29101 gacttggatg gcgttgtgcc ttgcgggcgc ggtagcggtc ggttatttcg gcattgcgta
    29161 tttcggtgca aaccccgaca aagtttcttc tatgagcggc aaccacgaac gcatcttcat
    29221 cgcgctttcc accctgctgt tcaacccttg gatcgccggc atcattttga gcgcgatttt
    29281 ggcggcggtg atgtccaccc tgtcctgcca gcttttggtt tgctccagcg cgattaccga
    29341 agacttctac aaaggctttt tgcgtaaaaa cgcgcaacag tcggaattgg tatgggtcgg
    29401 ccgcctgatg gtattggcga ttgccgtgat ttccatcctg attgcttccg atccgaacag
    29461 caaagtattg ggactggtgt cttacgcgtg ggcaggtttc ggtgcggcat tcggcccgat
    29521 tgtgattctg tccgtattgt ggaaacgcat cactgcctac ggcgcgttgt caggcatggt
    29581 tgcaggtgcg tcaaccgttg ttgtgtgggc ggaatgggta aaaaaaccgg ctcgagcggc
    29641 aggcgaaagc ggcttgttga cgatgtacga aatcgtcccg ggctttattg tttgcttgat
    29701 cgttgccgtg ttggtttccc tgttcaacaa agaaccttcg cgcgaaatcc aagaacgctt
    29761 tgaaaaagcc gacgcagatt accgcgccgc ccgataaacc cctttcagac ggcacggcat
    29821 cccggttcgt gccgtctgaa cccgcttccg ggccaaacac aaataaacct tgcctgcata
    29881 caggccttat ttgtgtttta cccgtttcat tttcaaagaa acggaagcac cgattctgcc
    29941 cgcatttgaa gccttgtatt tcatctgcgg atttattacg atattaccgt attcaggccg
    30001 caccgatgcc gtctgaaccc cccgaaaaaa ctttggagga tccaaaaaat gtttcatttt
    30061 gcatttccgg cacaaactgc cctgcgccaa gcgataaccg atgcctaccg ccgtaatgaa
    30121 atcgaagccg tacaggatat gttgcaacgt gcacagatga gtgacgaaga gcgcaacgcc
    30181 gcctccgagc ttgcccgccg tttggttacc caagtccgcg ccggccgcac caaagccggc
    30241 ggcgtggatg cgctgatgca cgagttttca ctctccagcg aagaaggcat cgcgctgatg
    30301 tgtctggcag aagccctgct gcgtatcccc gacaacgcca cgcgcgaccg cctgattgcc
    30361 gacaagattt cagacggcaa ctggaaaagc catttgaaca acagcccttc cctcttcgtc
    30421 aatgctgccg cctggggtct gctgattacc ggcaagctga ccgccacaaa cgacaaacaa
    30481 atgagttccg cactcggccg cctgatcggc aaaggcggcg caccgctcat ccgccaaggc
    30541 gtaaattacg ccatgcggct tctgggcaaa cagttcgtca ccggacagac cattgaagaa
    30601 gccctgcaaa acggcaaaga acgcgaaaaa atgggctacc gcttctcctt cgatatgttg
    30661 ggcgaagccg cctacaccca agccgatgcc gaccgctact accgcgacta tgtcgaagcc
    30721 atccacgcca tcggcaaaga tgcggcagga caaggcgttt acgaaggcaa cggcatttcc
    30781 gtcaaactct ccgccatcca tccgcgctac tcgcgcgccc aacacggccg cgtgatgggc
    30841 gaactgttgc ctcgcctgaa agaattgttc cttttgggta aaaaatacga tatcggtatc
    30901 aacatcgatg ccgaagaagc caaccgtctg gagctgtctt tggatttgat ggaggcactc
    30961 gtttccgacc ccgatttggc cggctacaaa ggcatcggtt tcgttgttca agcctaccaa
    31021 aaacgctgcc cgttcgtcat cgactatctg attgacctgg cccgccgcaa caaccaaaaa
    31081 ctgatgatcc gcctcgtcaa aggcgcgtat tgggacagcg aaatcaaatg ggcgcaagtg
    31141 gacggcttga acggctatcc gacctacacc cgcaaagtgc ataccgacat ctcctacctc
    31201 gcctgcgcgc gcaaactgct ttccgcgcaa gacgcggtat tcccgcaatt cgccacccac
    31261 aacgcctaca ccttgggcgc aatctaccaa atgggcaaag gcaaagattt tgaacaccaa
    31321 tgcctgcacg gtatgggcga aactttgtac gaccaagtcg tcggtccgca aaacttaggc
    31381 cgccgcgtgc gcgtgtacgc cccggtcggc acacacgaaa ccctgctcgc ctacttggtg
    31441 cgccgcctgt tggaaaacgg cgcgaactcg tctttcgtca accaaatcgt tgatgaaaac
    31501 atcagcatcg accgcctgat taagagcccg ttcgacacca tcgccgaaca aggcatccac
    31561 ctgcacaacg ccctgccgct gccgcgcgat ttgtacggca aatgccgtct gaactcgcaa
    31621 ggcgtggact tgagcaacga aaacgtattg cagcagcttc aagaacagat gaacaaagcc
    31681 gccgcacaag acttccacgc cgcatccatc gtcaacggca aagcccgcga tgtcggcgaa
    31741 gcgcaaccga ttaaaaaccc tgccgaccac ggcgatgttg tcggtacggt cagctttgcc
    31801 gatgccgcgc ttgcccaaaa agcggttggc gcagccgttg ccgcgttccc cgaatggagt
    31861 gcgacacctg ccgccgaacg cgccgcctgc ctgcgccgtt tcgccgacct gctcgaacag
    31921 cacacccccg cgctgatgat gcttgccgtg cgcgaagcgg gcaaaacgct gaacaacgcc
    31981 attgccgaag tgcgcgaagc cgtcgatttc tgccgctact acgcaaacga agccgaacac
    32041 accctgcctc aagacgcaaa agccgtcggc gcgattgtcg ccatcagccc gtggaacttc
    32101 ccgctcgcca tctttaccgg cgaagtcgtg tccgccctgg cggcaggcaa caccgtcatc
    32161 gccaaacctg ccgaacaaac cagcctgatt gccggttatg ccgtttccct catgcacgaa
    32221 gccggcatcc cgacttccgc cctgcaactc atcctcggcg caggcgacac gggtgcggca
    32281 ttgaccaacg atgccagcat cggcggcgtg attttcacag gctcgaccga agtggcgcgc
    32341 ctgatcaaca aagcccttgc caaacgcggc gacaatcccg tcctgattgc cgaaaccggc
    32401 ggacaaaacg ccatgattgt cgattccacc gcacttgccg aacaagtctg cgccgacgta
    32461 ttgaactccg ccttcgacag cgcgggacaa cgctgctccg ccctgcgtat cttatgcgtc
    32521 caagaagacg ttgccgaccg tatgctcgac atgatcaaag gcgcgatgga cgaactcgtc
    32581 gtcggcaaac cgattcagct cactaccgat gtcggccccg tcatcgatgc cgaagcgcag
    32641 caaaacctgt tgaaccacat caacaaaatg aaaggcgttg ccaagtctta ccacgaagtc
    32701 aaagccgccg ccgatgtcga ttccgaaaaa tccacgttcg ttcgccccat cctgtttgaa
    32761 ttgaacaacc tcaacgaact gcaacgcgaa gtcttcggcc ccgtcctgca cgtcgtccgc
    32821 taccgcgccg acgaactcga cagcgtcatc gaccaaatca acagcaaagg ctacgccctg
    32881 acccacggcg tacacagccg catcgaaggc acggtacgcc acatccgcag ccgcatcgaa
    32941 gccggcaacg tttacgtcaa ccgcaacatc gtcggtgcag tcgtcggcgt acagcccttc
    33001 ggcggacacg gtctgtccgg cacaggcccc aaagcaggag gttcgttcta cctgcaaaaa
    33061 ctgacccgca tccccgaatg ggttgccccg accctgagcc aaatcggaca ggcagacgaa
    33121 gccgcgctca aacgcctcga agccctgatt cacaaactgc cgttcaacgc cgaagagaaa
    33181 aaagccgcag cggccgcttt gggacacgcc cgcatccgca ccctgcgccg tgccgaaacc
    33241 gtccttaccg gcccgaccgg cgagcgcaac agcatctcat ggcacgcgcc aaaacgcgtt
    33301 tggatacacg gcggcagcac ggttcaagcc tttgccgcac tgaccggact tgccgcctcc
    33361 ggcgtacagg cagtggtcga acccgacagc ccgctggctt cctacaccgc cgacttggac
    33421 ggtttgctgc aaatcagcgg caaacccgaa aacaccggtg tgaaccacgt cgccgcccta
    33481 agcccactgg acagcagccg caagcaggaa ctggcaaacc gcgacggcgc actcatccgc
    33541 atcctcccgt ccggacaagg cttggacatc ctgcaagtgt ttgaagaaat ttcttgcagc
    33601 atcaacacca cagccgccgg cggcaacgcc ggcctgatgg cggttgccga ctgattttgc
    33661 caaaccgccc gggcgcggcc cgtgaacaaa tgccgtctga aaacctttca ggcggcattt
    33721 tatagtggat taacaaaaat caggacaagg cggcgagccg cagacagtac aaatagtaca
    33781 gcaaggcggg ccaacgctgt actggtttaa attcaattca ctatatgtgt tggcacatcg
    33841 ctccaaacct gatataatcc gcctttcaac atcagtgaaa atcgttcttt ttagtcagtt
    33901 aacattaaat ttcggagtcg aaaaatgaat ccagccccaa aaaacttctc ttctcttctc
    33961 ttctcttctc ttctcttctc ttctcttctc ttctcttccg cagcgcaggc ggcaagtgaa
    34021 ggcaatggcc gcggcccgta tgtgcaggcg gatttagcct acgccgccga acgcattacc
    34081 cacgattatc cgaaaccaac cgatccaagc aaaggcaaaa taagcacggt aagcgattat
    34141 ttcagaaaca tccgtacgca ttccatccac cccagggtgt cggtcggcta cgacttcggc
    34201 ggctggagga tagcggcaga ttatgcccgt tacagaaaat ggaacaacag taaatattcc
    34261 gtcagcataa aagagttgaa aaataataat aagaagaaga cggaaaatca ggaaaacggc
    34321 agcttccacg ccgtttcttc tctcggctta tcagccgttt acgatttcaa actcaacgat
    34381 aaattcaaac cctatatcgg cgcgcgcgtc gcctacggac acgtcagaca cagcatcgat
    34441 tcgactaaaa aaataacagc cggtgctggt ggcgcagggt ctccggttcg tccttcttac
    34501 aaaagtacgc aagacgccca tcaccaaagc aacagcatcc gccgcgtggg tctcggcgtc
    34561 atcgccggcg tcggtttcga catcacgccc aagctgaccc tggacaccgg gtaccgctac
    34621 cacaactggg gacgcttgga aaacacccgc ttcaaaaccc acgaagcctc attgggcgtg
    34681 cgctaccgct tctgattc
//
LOCUS       DAWTQS010000016        31810 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.016, whole genome shotgun sequence.
ACCESSION   DAWTQS010000016 DAWTQS010000000
VERSION     DAWTQS010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 31810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31810
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.016"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(106..1137)
                     /gene="gap"
                     /locus_tag="WNA25_000771"
     CDS             complement(106..1137)
                     /gene="gap"
                     /locus_tag="WNA25_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFA9823578.1"
                     /translation="MGIKVAINGYGRIGRQVLRAIYDYQIQDQLQIVAVNASGSLETN
                     AHLTKFDTVHGRFEADVSHDGGNLIVNGDKIPFFSTRNPAELPWKELGVDLVMECTGA
                     FTSKEKAKIHLESGAKKVLISAPGGDDVDATVVYGVNDSVLTADMTVVSNASCTTNCL
                     SPVAKVLSESVGIVKGAMTTIHALTNDQTVTDVRHKDLRRARSGVENMIPTKTGAAKA
                     VGLVLPELKGRLDGLAIRVPTVNVSLVDLSFQAARDTTVEEINALMKAVSEAGALKGV
                     LGYNTLPLVSMDFNHTTEASHFDATLTKVVDGNMVKVFAWYDNEWGFSCQMLNTARRM
                     FGLEVRPLK"
     gene            complement(1336..2175)
                     /locus_tag="WNA25_000772"
     CDS             complement(1336..2175)
                     /locus_tag="WNA25_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HFA9823579.1"
                     /translation="MTATAADLDRLLPQTQCRECGYEGCLPYARAMLRGEAHNLCAPG
                     GATVVRDLAALLGKPLVAPAKTQAKALARIDETACIGCTACIRACPADAIMGAGKLMH
                     TVITDECTGCGLCVAPCPVDCIHMQPVADTVLPRARRFSLSDDSRFAAAEHARARYLK
                     RNERKQREADERKAMLAEREAAVRNARPQTPDTPEKPAFNPADLIAKAMAKAQTQQDR
                     LAAADNRQGYQAKQIAEARERAELRRAQRDMKYGSDSEKAAALEYLKQYKAKQEAAQN
                     TAS"
     gene            2321..4297
                     /locus_tag="WNA25_000773"
     CDS             2321..4297
                     /locus_tag="WNA25_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFA9823580.1"
                     /translation="MNEFSLAPIVVVLLVSVITVILCRKFNIPSMLGYLLVGFLAGPG
                     MLSLIPKSHATDYLGEIGIVFLMFSIGLEFSLPKLRAMRRLVFGLGGLQVGVTMLSVM
                     GILMLTGVPFNWAFAVSGALAMSSTAIVSRILSEKTELGQPHGQMAMGVLLMQDIAVV
                     PLMILIPALAGGGDGNIWAALGLAFAKMLLTLGLLFFVGSKIMSRWFRMVAKRKSSEL
                     FMINVLLVTLGVAYLTELEGLSMALGAFVAGMLLSETEYRFQVEDDIRPFRDILLGFF
                     FITVGMKLDIQALIGGWRQVLMLLAMLLVLKALVVFAIAFKMKHSVGDSLKTALYLAQ
                     GGEFGFVMLAIAGQLDMVSPELEQAATAAVLLSMIIAPFLLGGSDALVGRLVKSSWDM
                     KSLDLHSMLVEAMSKSDHVLIVGFGRGGQTVGRVLAQEDIPYFALDLDIARVQVARSA
                     GEPVSFGDAKRREVLEAAGLGRAKMVVVTLNNMHETQHVLDNVLSMHPNMPVYARATN
                     DDYVKTFTDMGAEEAVSDTKETGLVLAGYAMLGNGASYRHVYQTMANIRHSRYAALEG
                     LFVGSDDEAGFGENGETVRHAFPLAAEAYAVGKTVGTLPMAAYGIKLLFVRRRTGRIE
                     NPDASFTLEGGDVLVVAGKKEEIISFENWSLQGI"
     gene            4367..4442
                     /locus_tag="WNA25_000774"
     tRNA            4367..4442
                     /locus_tag="WNA25_000774"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4400..4402,aa:Val,seq:tac)
     gene            4475..4551
                     /locus_tag="WNA25_000775"
     tRNA            4475..4551
                     /locus_tag="WNA25_000775"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4509..4511,aa:Asp,seq:gtc)
     gene            4591..4666
                     /locus_tag="WNA25_000776"
     tRNA            4591..4666
                     /locus_tag="WNA25_000776"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4624..4626,aa:Val,seq:tac)
     gene            4699..4775
                     /locus_tag="WNA25_000777"
     tRNA            4699..4775
                     /locus_tag="WNA25_000777"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4733..4735,aa:Asp,seq:gtc)
     gene            5347..5445
                     /locus_tag="WNA25_000778"
     CDS             5347..5445
                     /locus_tag="WNA25_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="eamA-like transporter family protein"
                     /protein_id="HFA9823581.1"
                     /translation="MAVVFLDERPNTQEWIGLGLVTAGVLTLALKR"
     gene            complement(5516..6901)
                     /locus_tag="WNA25_000779"
     CDS             complement(5516..6901)
                     /locus_tag="WNA25_000779"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFA9823582.1"
                     /translation="MISIFDIFKIGIGPSSSHTVGPMKAAAAFAAGLDAQTARIAIDI
                     YGSLALTGYGHGTFDALMLGLEGSLPHDIPLAGIPERLGRIRTQHILRLNGQEIRFIP
                     DRDLNIRGNQVLPKHPNGLRFTAYASDGTVLKEQVYYSVGGGFVVTEEDFEWQSETEK
                     AVPYPYNSCAGLLARCRLNRLDISEVVLANEAALAGCGEAEIRRRVAGVAEIMEECIK
                     RGLGADGELPGGLNVRRRAPQLAAKLKVLRETEIVNTQLWPMVYAMAVNEENAAGGRV
                     VTAPTNGAAGIIPAVLHYFRKFNPHATQERVENFLLTAGAIGILYKTNASISGADVGC
                     QGEVGVACSMAAGAYAEVIGGTPKQVENAAEMAMEHHLGLTCDPVGGLVQIPCIERNG
                     IAAEKALKLGTLALLEDGTDKKVSLDEVIRTMLQTGRDMKATYKETSLAGLAATLRKK
                     AVPVSVRVVEC"
     gene            complement(7000..9390)
                     /gene="gyrB"
                     /locus_tag="WNA25_000780"
     CDS             complement(7000..9390)
                     /gene="gyrB"
                     /locus_tag="WNA25_000780"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFA9823583.1"
                     /translation="MTEQKHEEYGADSIQVLEGLEAVRKRPGMYIGDTQDGSGLHHMV
                     FEVLDNAIDEALAGHCDKITVTIHADHSVSVADNGRGMPTGIHPKEGRSAAEVIMTVL
                     HAGGKFDNNSYKISGGLHGVGVSVVNALSDWVTLTIYRDGKEHFVRFVRGETEEPLKI
                     VGDSDKKGTTVRFLAGTETFGNIEYSFDILAKRIRELSFLNNGVDIELTDERDGKHES
                     FALSGGVAGFVQYMNRKKTPLHEKIFYAFGEKDGMSVECAMQWNDSYQESVQCFTNNI
                     PQRDGGTHLTALRQVMTRTINSYIEANEVAKKAKVETAGDDMREGLTCVLSVKLPDPK
                     FSSQTKDKLVSGEIGPVVNEVINQALTDFLEENPNEAKIITGKIVDAARAREAARKAR
                     EITRRKGVMDGLGLPGKLADCQEKDPALSELYLVEGDSAGGSAMQGRDRKFQAILPLK
                     GKILNVEKARFEKMLASQEVATLITALGAGIGKEEFNPEKLRYHRIIIMTDADVDGAH
                     IRTLLLTFFYRQMPELVERGYIYIAQPPLYKAKYGKQERYLKDELEKDQWLLGLALEK
                     AKIVSDGRTIEGAELADTAKQFLLAKTVIEQESRFVDELVLRAMLHASPIDLTSSENA
                     DKAVAELSGLLDEKEAALERIEGHEGHRFIKITRKLHGNVMVSYIEPKFLNSKAYQTL
                     TQTAAALKGLVGEGAKLYKGENEYDADSFETALDILMSVAQKGMSIQRYKGLGEMNPE
                     QLWETTMDPAVRRLLKVRIEDAIAADEVFVTLMGDEVEPRRAFIENNALIAQNIDA"
     gene            9656..10528
                     /locus_tag="WNA25_000781"
     CDS             9656..10528
                     /locus_tag="WNA25_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFA9823584.1"
                     /translation="MEIWNMLNTWPDAVPIRAEAAESVAAVAALLLARALLLNIHFRR
                     HPDFGIESKRRFLVASRNITLLLVLFSLAFIWSAQIQTLALSMFAVAAAVVVATKELI
                     MCLSGSILRSATQQYSVGDYIEINGLRGRVVDINLLNTLMMQVGPNPLVGQLAGTTVS
                     FPNSLLLSHPVRRDNILGDYVIHTVEIPVPIHLDSDEAVCRLKAVLEPLCAPYIPAIQ
                     RYLENVQAEKLFITPAARPRVTRVPYDDKAYRIIVRFASPVSKRLEIQQAVMDEFLRV
                     QYRLLNHPAGSETL"
     gene            10616..12652
                     /locus_tag="WNA25_000782"
     CDS             10616..12652
                     /locus_tag="WNA25_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HFA9823585.1"
                     /translation="MIDNALLHLGEEPRFNQIKTEDIKPAVQTAIAEARGQIAAVKAQ
                     THTGWANTVERLTGITERVGRIWGVVSHLNSVVDTPELRAVYNELMPEITVFFTEIGQ
                     DIELYNRFKTIKNSPEFATLSPAQKTKLDHDLRDFVLSGAELPPERQAELAKLQTEGA
                     QLSAKFSQNVLDATDAFGIYFDDAAPLAGIPEDALAMFAAAAQSEGKTGYKIGLQIPH
                     YLAVIQYAGNRELREQIYRAYVTRASELSNDGKFDNTANIDRTLENALKTAKLLGFKN
                     YAELSLATKMADTPEQVLNFLHDLARRAKPYAEKDLAEVKAFAREHLGLADPQPWDLS
                     YAGEKLREAKYAFSETEVKKYFPVGKVLNGLFAQIKKLYGIGFAEKTVPVWHKDVRYF
                     ELEQNGKTIGGVYMDLYAREGKRGGAWMNDYKGRRRFADGTLQLPTAYLVCNFAPPVG
                     GKEARLSHDEILTLFHETGHGLHHLLTQVDELGVSGINGVEWDAVELPSQFMENFVWE
                     YNVLAQMSAHEETGEPLPKELFDKMLAAKNFQRGMFLVRQMEFALFDMTIYSEDNEGR
                     LKNWQQVLDSVRKEVAVIQPPEYNRFANSFGHIFAGGYSAGYYSYAWAEVLSADAYAA
                     FEESDDVAATGKRFWQEILAVGGSRSAAESFKAFRGREPSIDALLRHSGFDNAA"
     gene            complement(13233..14396)
                     /locus_tag="WNA25_000783"
     CDS             complement(13233..14396)
                     /locus_tag="WNA25_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HFA9823586.1"
                     /translation="MSFKLRYLASVLALSSLLAACGGQEKSAAGDASPASETEAASQV
                     QASEAVPSASSASPEDQDLLKRAQGVFQPLPTVEEMQKIRPFTEEQVKLGHQLWYEPR
                     LSKGNTVSCNSCHNLASAGVDNMPTSQGHKGQFGGRNSPTALNAALLGSQFWDGRAAD
                     VEEQAGGPLVNPVEMANDSQEAAAAKIAKVPEYQEMFKKAFPEDGAVSFKNITTALGA
                     FERTLLTPTKWDEYLKGNVNALSEQERKGVRAFMDNGCIACHNGVNLGGTTFQKFGLV
                     QGPYWKFIEDPKRDKGRADVTKKTEDEFFFRVPGLRNVAKTYPYFHNGSVWELDKAVT
                     IMGKAQLGKDIPKEDVDNIVVFLNALSGNVSESARTMPELPLTAPMESKPDNK"
     gene            complement(14618..15184)
                     /locus_tag="WNA25_000784"
                     /pseudo
     CDS             complement(14618..15184)
                     /locus_tag="WNA25_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
     gene            complement(15700..17214)
                     /gene="katA"
                     /locus_tag="WNA25_000785"
     CDS             complement(15700..17214)
                     /gene="katA"
                     /locus_tag="WNA25_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase KatA"
                     /protein_id="HFA9823587.1"
                     /translation="MTTSKCPVTHLTMNNGAPVADNQNSLTAGPRGPLLTQDLWLNEK
                     LADFVREVIPERRMHAKGSGAFGTFTVTHDITKYTRAKIFSEVGKKTEMFARFTTVAG
                     ERGAADAERDIRGFALKFYTEEGNWDVVGNNTPVFFLRDPRKFPDLNKAVKRDPRTNM
                     RSATNNWDFWTLLPEALHQVTIVMSDRGIPASYRHMHGFGSHTYSFWNEAGERFWVKF
                     HFRSQQGIKNLTNEEAAKIIADDRESHQRDLYEAIERGEFPKWTMYIQVMPEADAEKV
                     PYHPFDLTKVWPKKDYPLIEVGEFELNRNPENFFADVEQSAFAPSNLVPGIGASPDKM
                     LQARLFNYADAQRYRLGVNFRQIPVNRPRCPVHSNQRDGQGRADGNYGSLPHYEPNSF
                     GQWQQQPDFAEPPLKINGDAAHWDYRQDDDDYFSQPRALFNLMNDAQKQALFDNTAAA
                     MGDAPDFIKYRHIRNCYRCDPAYGEGVAKALGLTVEDAQAARATDPALGQAGLL"
     gene            17564..18414
                     /locus_tag="WNA25_000786"
                     /pseudo
     CDS             17564..18414
                     /locus_tag="WNA25_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
     gene            complement(18816..19943)
                     /locus_tag="WNA25_000787"
     CDS             complement(18816..19943)
                     /locus_tag="WNA25_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFA9823588.1"
                     /translation="MKIVFITTVASSIYGFRAPVIKKLIGKNHQVYAFVSEFSDNELD
                     IIREMGVTPVTYRSNRSGLNPFSDIKSTFLIFKALKKISPDLVVPYFAKPVIFGTFAA
                     KLAGVPRIVGMLEGLGFAFTPQPEGIPLKTKIIKGILIALYRITLPMLESLIVLNPDD
                     KDELLHQYGIKIKNIHILGGIGLDLRQYPYSEADIPDEKEPVKFLFIGRFLKEKGIDE
                     FIRAAEQVKGKYPDTVFTALGAIDKPRGSGDLEQPAARDIIRFPGFVNNVSEMIKEHH
                     IFVLPSYYREGVPRSTQEAMAVGRAVITTDVPGCRETVADKVNGFLIEPWNPRILAEK
                     MIYFIENRAAVRLMGNASYAIAKDKFDAEKVDLKFLDILKA"
     gene            complement(20297..21522)
                     /locus_tag="WNA25_000788"
                     /pseudo
     CDS             complement(20297..21522)
                     /locus_tag="WNA25_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259190.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(21915..23162)
                     /gene="fabF"
                     /locus_tag="WNA25_000789"
     CDS             complement(21915..23162)
                     /gene="fabF"
                     /locus_tag="WNA25_000789"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFA9823589.1"
                     /translation="MSQRRVVITGLGQVSPVGNTAAEAWDNLLAGKSGIGAITRFDAS
                     DINSRVAGEVRGFDIGQYISAKEARRMDVFIHYGIAAALQAIADSGLDDVENLDKDRI
                     GVNIGSGIGGLPSIEATGKAVIEGGARKINPFFIPGSLINLISGHVTILKGYRGPSYG
                     MVSACTTGAHAIGDSARLIKYGDADIMVAGGAEGAISTLGVGGFAAMKALSTRNDDPA
                     TASRPWDKGRDGFVIGEGAGILVLEELEHAKKRGAKIYAEIVGFGMSSDAYHITAPNE
                     EGPALAVTRALKDAGINPEDVDYVNAHGTSTPLGDANETKALKRAFGEHACKTVISST
                     KSMTGHLLGAAGGVEAVYSILAIHDGKIPPTINIFEQDVEAGCDLDYCANEARDAEID
                     VAISNSFGFGGTNGTLVFKRFKG"
     gene            complement(23319..23555)
                     /gene="acpP"
                     /locus_tag="WNA25_000790"
     CDS             complement(23319..23555)
                     /gene="acpP"
                     /locus_tag="WNA25_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFA9823590.1"
                     /translation="MSNIEQQVKKIIAEQLGVNEADVKNESSFQDDLGADSLDTVELV
                     MALEEAFGCEIPDEDAEKITTVQLAIDYINAHNG"
     gene            23787..24794
                     /locus_tag="WNA25_000791"
     CDS             23787..24794
                     /locus_tag="WNA25_000791"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFA9823591.1"
                     /translation="MYPLARRILFALDAEKAHHFTLDALNTVYKLGLIPVTDNRTKPI
                     KLMGMDLPNPVGLAAGLDKNGEYIDALGALGFGFLEIGTVTRNPQPGNPQPRLFRVPE
                     HQGIINRMGFNNHGIDAMIRNIEKSKYQGVLGINIGKNAVTPIQNAADDYLICLEKAY
                     AHASYITVNISSPNTKNLRALQGGGELGALLEALKNKQAQLAAAHGKYIPLAVKIAPD
                     LDEAQIEDIARVVKSVEMDGIIATNTTIDKSSLGSHPLAGEQGGLSGFPVREKSNRVL
                     KKLAEHIDGALPIIGVGGIMEGGDAAEKIRLGTTAVQVYSGLIYKGPALVKECLKALA
                     R"
     gene            24991..25464
                     /locus_tag="WNA25_000792"
     CDS             24991..25464
                     /locus_tag="WNA25_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2247 family protein"
                     /protein_id="HFA9823592.1"
                     /translation="MNLDLTAQKVRLSWKDILWGYENKYLGWADVAAYARKMTLSDHD
                     ERVFKLSLTNKSNIFELKPVLEDLASETRGYSPKNWLYILLNDVFHRKEEFEDPLGEV
                     EKIYADFDYPEEIESFVRYMPPKDGYIPSNHSYEENIARLYSHWEHYLNNGGGQG"
     gene            25478..25813
                     /locus_tag="WNA25_000793"
     CDS             25478..25813
                     /locus_tag="WNA25_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823593.1"
                     /translation="MPSEALSGVQTAFCFPTVYKLSLFLDRNNDLIYLKRLEDMIMGF
                     WGSVGKAAKAVGEGLIEAGNQHKALKMEYAEKSSEELHEIVKSDGFFKNSTREKGAAY
                     AVLKERGEV"
     gene            26007..28697
                     /gene="glnE"
                     /locus_tag="WNA25_000794"
     CDS             26007..28697
                     /gene="glnE"
                     /locus_tag="WNA25_000794"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFA9823594.1"
                     /translation="MSDHRLDTARRHSLFLARQLDNGKLKPEIFLPMLDKALTDEDFQ
                     AFADWDKIRAEESEEELARQLRELRRYVVSQIIVRDINRISDLNEVTRTITLFADFAV
                     NTALDFAYAYYQDMYGTPIGRYTKSPQHLSVVAMGKAGGYELNVSSDIDLIFVYPESG
                     DTDGRRERGNQEFFTKVGQKLIALLNGITADGQVFRVDMRLRPDGDSGALVLSETALE
                     QYLITQGREWERYAWCKGRVVTPYPNDIKSLVRPFVFRKYLDYGAYEAMRNLHRQIRS
                     EVSKKGMADNIKLGAGGIREVEFIAQIFQMIRGGQMRALQLKGTQETLKKIAEMGIMP
                     SENVETLLAAYRFLRDVEHRLQYWDDRQTQTLPISPEQRQLLAESMGFDSYAAFSDGL
                     NVHRNKVNQLFNEILSEPEEQAQSNSEWQWAWQEKPDEEERLGRLKEHGFDAEAVTAR
                     LEQICHGHKYRRLSAHAQPRFDTIVPLFVQAAAEQNNPTDTLMRLLDFLENISLRSAY
                     LAFLNEHPQTLAQLAQIMSQSSWVAAYLNKYPILLDELISAQLLDTAFDWQALAAALS
                     DGIEACGGDTEAQMDTLRHFQHAQVFRLAVQDLAGLWTVESLSDQLSALADTVIAAAL
                     SCAWADMPKKHRDTPQFAVIGYGKLGGKELGYASDLDLVYLYDDPHPEAGDVYSRLAR
                     RLTNRLSAATGAGSLYETDLRLRPNGDAGFLAHSIAAFGKYQRENAWTWEHQSLTRAR
                     FICGTPEIQTAFDRIRTEMLTAERDQTALAGEIIEMREKMFPTHPPADSNVKYARGGV
                     VDVEFIVQYLILAHARQYPQLLDNYGNIALLNIAADCGLIDKTLAGQSRTAYRLYRRQ
                     QHNTKLRDAAKTEVSDELLSHYGNVRKLWREVFGEEAATA"
     gene            29121..31328
                     /gene="uvrD"
                     /locus_tag="WNA25_000795"
     CDS             29121..31328
                     /gene="uvrD"
                     /locus_tag="WNA25_000795"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFA9823595.1"
                     /translation="MFPDQSAPNLLQGLNPEQLSAVTWPPQSALVLAGAGSGKTRVLT
                     TRIAWLLQSGQASVHSIMAVTFTNKAAKEMQTRFGAMIPINVRAMWLGTFHGLCHRFL
                     RLHHRDAGLPSSFQILDSGDQLSLIKRLLKSLNIAEEIIAPRSLQGFINAQKESGLRA
                     SVLGAPDPHTSRMIGCYAEYDKICQREGVVDFAELMLRSYEMLQSNEILRRHYQNRFN
                     HILVDEFQDTNKLQYAWLKLMAGGNAAVFAVGDDDQSIYRFRGANVGNMTALMEEFHI
                     DAPVKLEQNYRSVGNILAAANAVIENNDERLGKNLRTDAEAGDKIRYYSAFTDLEEAR
                     FIVDETKALEREGWDLDEIAVLYRSNAQSRVIEQSLFRSGIPYKIYGGLRFYERQEIK
                     HALAYLRLAVNPDDDNALLRVINFPPRGIGARTVENLQTASNEQGITLWQAACNAGAK
                     AAKVAAFVRLIEALRNQVGQMHLSEIIVGILKDSGLTEHYRTQKGDNQDRLDNLDELV
                     NAAIEFKPEDSNFETLPENISDDPAFPILAFLSNAALESGENQAGAGEKAVQLMTVHA
                     AKGLEFNAVFLTGMEEGRFPSEMSLAERGGLEEERRLMYVAITRARKRLYITMAQQRM
                     LHGQTQFGIASRFVEEIPPEVLHYLSVKKPAFDSYGNTRQTTVQRDKIIDDFKQPQTY
                     AGFRIGQNVRHAKFGTGVIIDAADKGESARLTINFGKQGVKELDTKFAKLEAM"
BASE COUNT         7054 a         8564 c         8904 g         7288 t
ORIGIN      
        1 tggaaatgac gacagccggg taaaatcgcg gtcaaaaatg aaaagtgccg tctgaaactt
       61 gaaaacatcg ggtttcagac ggcattttgt ttgacggttt gttgcttatt tgagcgggcg
      121 cacttcaagt ccgaacatac ggcgtgcggt gttcagcatt tggcagctga agccccattc
      181 gttgtcatac cacgcgaaca ctttgaccat gttgccgtcg acgactttgg tcagcgttgc
      241 gtcgaagtgg ctggcttcgg tggtgtggtt gaagtccatg gaaaccaatg gcagggtgtt
      301 gtagcccaaa acgcctttga gcgcgcctgc ttccgagacg gctttcatca gtgcgttgat
      361 ttcttcgact gtggtgtcgc gcgcggcttg gaagctcaaa tctaccaagg atacgttgac
      421 ggtcggcacg cggatggcaa gcccgtcgag tctgcctttc aattcgggca agaccaaacc
      481 gacggctttt gccgcgccgg ttttggtcgg aatcatgttt tccacgccgc tgcgggcgcg
      541 gcgcaggtct ttgtggcgca cgtcggtaac ggtttggtcg ttggtcagtg cgtggatggt
      601 ggtcattgcg cctttgacga tgccgacgct ttcgctcaac actttggcaa ccggcgagag
      661 gcagttggtg gtgcaggaag cgttggaaac gacggtcatg tcggcggtca ggacgctgtc
      721 gttcacgccg tacacgacgg ttgcatcgac atcgtcgccg cccggtgcgg aaatgaggac
      781 ttttttcgcg ccgctttcga ggtggatttt ggctttttct ttgctggtga acgcgccggt
      841 gcattccatg accaaatcga caccgagttc tttccacggc agttcggcag ggttgcgggt
      901 cgagaagaag gggattttgt cgccgttgac gatgaggttg ccgccgtcgt gggatacgtc
      961 ggcttcaaag cgtccgtgta cggtgtcgaa tttggtcaga tgggcgttgg tttcaaggct
     1021 gccgctggcg ttgacggcga cgatttggag ttggtcttga atctgatagt cgtagatggc
     1081 gcgcaaaacc tggcggccga tgcgtccgta gccgttgatg gcgactttga tgcccatggt
     1141 ttgttccttt gttgagggtt gggtagattt tcggggcgga ttatagcaaa tttgtagtgg
     1201 cgtgtaatta atattttatt gaaaacggca cggccggaag ggtgggcggt aagatacgga
     1261 cggcacgggt acggcggacg gagagcttga taaaatgccg tctgaagcgg cttcagacgg
     1321 catgtcgggt aagggtcagg aggcggtatt ctgtgcggct tcctgtttgg ctttgtattg
     1381 tttgaggtat tcgagggcgg cggctttttc gctgtcgctg ccgtatttca tatcgcgttg
     1441 ggcgcggcgc aattcggcgc gttcgcgggc ttcggctatc tgttttgcct gatagccttg
     1501 gcggttgtcg gcggcggcga ggcgatcctg ttgggtttgc gcttttgcca tggctttggc
     1561 gatgaggtcg gcggggttga acgccggttt ttccggtgtg tcgggcgttt gcggacgcgc
     1621 gttgcggacg gcggcttcgc gttcggcaag catggccttg cgttcgtcgg cttcgcgctg
     1681 tttgcgttcg ttgcgtttga ggtagcgcgc acgcgcgtgt tcggcggcgg caaaacggct
     1741 gtcgtcggac aggctgaaac ggcgcgcgcg cggcaggacg gtgtcggcaa cgggctgcat
     1801 atggatgcag tccacggggc agggggcgac gcagagtccg cagccggtgc attcgtcggt
     1861 gatgacggtg tgcataagtt tgcccgcgcc cataatggca tcggcagggc aggcgcggat
     1921 gcaggcggtg cagccgatac aggcggtttc gtctatccgg gcgagtgctt tggcttgggt
     1981 tttggcaggt gcgacaaggg gtttgccgag cagggcggca aggtcgcgga cgacggttgc
     2041 gccgcccggg gcgcagaggt tgtgcgcctc gccccgcagc atcgcgcggg cgtagggcag
     2101 gcagccttca tagccgcatt cgcggcattg ggtttggggg aggagtcggt ctaaatcggc
     2161 ggcggtggcg gtcattgtgc ggtggtgcgg gtcaaaaggg cgtattttat cagaattgta
     2221 tgccgcgccc gtttcggatg gtgcgcggtg ttttgttata atacggcggc gtatgccgtt
     2281 tcagacggca tttttctgta ttttcctgtt cggacggtcc atgaacgaat tttcgcttgc
     2341 ccctattgtg gttgttctgc tggtgtcggt cattacggtg atcctgtgcc gcaagttcaa
     2401 cattccctcc atgctgggct acctgctggt gggctttttg gcggggcccg gtatgctcag
     2461 cctgattccg aaaagccatg cgacggatta tttgggcgaa atcgggattg tgttcctgat
     2521 gttcagcatc ggtttggagt tttcattgcc caagctgagg gcgatgaggc ggctggtgtt
     2581 cggtctgggc ggtttgcagg tcggcgttac gatgctgtcg gtaatgggca tactgatgct
     2641 gacgggcgtg ccgttcaatt gggcgtttgc cgtatcgggc gcgttggcga tgtcgtccac
     2701 ggcgattgtg agccggattt tgtcggaaaa gacggagttg gggcagccgc acggtcagat
     2761 ggcgatgggc gtgctgctga tgcaggacat cgccgtcgtg ccgctgatga ttctgattcc
     2821 cgcgctggct ggcggagggg acggaaatat ttgggcggcc ttgggtttgg cgtttgcaaa
     2881 aatgctgctg acgctggggc tgctgttttt cgtcggcagc aaaattatgt cgcgatggtt
     2941 caggatggtg gcaaaacgca aatcgtccga actctttatg atcaatgtgc tgctggtaac
     3001 cttgggtgtg gcttatctga ctgagctgga aggtttgtct atggcgttgg gcgcattcgt
     3061 tgccggcatg ttgctttcgg aaacggaata ccgtttccaa gtcgaagacg acattcgccc
     3121 gttccgcgat attttgctcg gcttcttctt tatcacggtc ggcatgaagc tggacattca
     3181 ggcattgatc ggcggctggc ggcaggtatt gatgctgttg gcaatgctgc tggtgttgaa
     3241 ggcattggtt gtgtttgcca ttgcattcaa aatgaagcat tcggtcggcg acagcctcaa
     3301 aacggctttg tatctcgcgc aaggcggcga gttcggcttc gtgatgctgg ccattgccgg
     3361 gcagcttgat atggtttcgc cagaattgga acaggcggcg acggcggcgg ttctgctgtc
     3421 gatgattatc gcgcccttcc tcttgggcgg cagcgatgcg ctggtcgggc gcttggtcaa
     3481 gtcaagctgg gacatgaagt cgctcgatct gcacagcatg ttggtggaag cgatgagcaa
     3541 gtctgatcat gtgctgattg tcggcttcgg caggggcggg cagacggtcg gacgcgtcct
     3601 cgcccaagag gatattccgt atttcgcgct cgacttggac atcgcacgcg tgcaggtagc
     3661 cagaagtgca ggcgagccgg tgtcgttcgg cgatgcgaaa cgcagggaag tattggaagc
     3721 cgccggtctg ggacgggcga aaatggtggt ggttacgctc aacaatatgc acgaaacgca
     3781 acacgtttta gacaatgtgc tgtccatgca tcccaatatg cccgtatatg cgcgcgccac
     3841 caatgacgat tatgtgaaaa cgtttaccga tatgggtgcg gaagaagccg tgtcggacac
     3901 caaagaaacc ggactcgtgc tggcaggcta tgcgatgttg ggtaacggcg cgtcgtaccg
     3961 gcacgtctat cagacgatgg caaatatccg ccacagccgt tatgccgcgt tggagggact
     4021 gtttgtcggt agtgatgatg aggcaggatt cggcgaaaat ggcgaaaccg tccgccacgc
     4081 ctttcctttg gctgcagaag catacgccgt cggcaaaaca gtcggcacgc ttccgatggc
     4141 ggcttacggc atcaaactct tgttcgtccg ccgccgcacc ggccggattg aaaacccgga
     4201 tgcctcgttt acattggaag gcggtgacgt gttggtggtc gcaggcaaaa aagaagaaat
     4261 tatctctttt gaaaactgga gcttgcaggg aatataaatg aaatgccgaa ataaggcttg
     4321 cgccattccc ggttatttgg tctaatagcg ttttcgcaaa tcgcaagggt gattagctca
     4381 gttggtagag cgtctgcctt acaagcagaa tgtcggcggt tcgactccgt catcacccac
     4441 caagtttcct ttcattgttg caaacaatgg atgcgcggtg gtagctcagt tggttagagt
     4501 accggcctgt cacgccggtg gtcgcgggtt cgagccccgt ccgccgcgcc aagtttcaaa
     4561 atactgactc tgtcggtatt ttttatacac gggtgattag ctcagttggt agagcgtctg
     4621 ccttacaagc agaatgtcgg cggttcgact ccgtcatcac ccaccaagtt tcctttcatt
     4681 ggtgcaaaca atggatgcgc ggtggtagct cagttggtta gagtaccggc ctgtcacgcc
     4741 ggtggtcgcg ggttcgagcc ccgtccgccg cgccaaaagt taagaaatac caacctccgg
     4801 ttggtatttt ttgtttgtac gctttgaaaa atgtttgttt ccggattttg ccattcccat
     4861 ccggttttgc gctgtacgat gtgttttagc gcggacttga tcaaaatcgg atgtggttcc
     4921 ggtatttgag gctttgatta gggatgcgga ctttcaatat atttttctca gttacaacaa
     4981 cgaagtcttg atgtctgtcg ggcaggtaag ggagattttt gagtgtttcg gcaaatataa
     5041 tttggttcaa acggaatacc ggcgttttaa ggcggataag acagaaaacc gtaatcataa
     5101 ggcaaattcg acattcgaat atctgcatat tttagaaaag accttttata gtggattaac
     5161 aaaaaccggt acggcgttgc ctcgccttag ctcaaagaga acgattctct aaggtgctga
     5221 agcaccaagt gaatcggttc cgtactattt gtactgcctg cggcttcgtc gccttgtcct
     5281 gatttttgtt aatccactat aaaaattctt gccggatgct gcaaacaacg ccggtttgca
     5341 ttcctgatgg cggtggtttt cttggacgaa cgcccgaaca cgcaggaatg gataggcttg
     5401 gggctggtta cggcgggcgt gttgacgttg gcgttgaagc ggtaaacccg caagaaataa
     5461 atgaaatgcc gtctgaaaaa ctgttttcag acggcatttt cgtttctgtc catcctcagc
     5521 actcgaccac gcgcacggat acggggacgg cttttttccg gagcgtggcg gcgagtccgg
     5581 caagcgaggt ttctttgtag gtcgccttca tgtcccggcc ggtttgcagc atggttcgga
     5641 tgacttcgtc gagcgagact tttttgtccg tgccgtcttc caagagcgcg agcgtgccga
     5701 gtttgagggc tttttcggcg gcgatgccgt tgcgttcgat gcaggggatt tgcaccaacc
     5761 cgccgacggg gtcgcaagtt aagcccaaat ggtgttccat tgccatttcg gcggcgtttt
     5821 ccacttgttt gggcgtgccg ccgatgactt cggcgtatgc acccgccgcc attgaacacg
     5881 ctacgccgac ttcgccctga caaccgacat ccgcgccgga aatggaggcg ttggttttgt
     5941 agaggatgcc gattgcgcct gcggtgagca ggaagttttc gacacgttcc tgtgtggcgt
     6001 gggggttgaa cttgcggaaa taatgcaata cggcgggaat gatgcctgcc gcgccgttgg
     6061 tcggggcggt aacgacgcgt ccgccggcgg cgttttcttc gttgaccgcc atggcgtaca
     6121 ccatcggcca gagctgggtg ttgacgattt cggtttcgcg caggactttg agtttggcgg
     6181 caagctgcgg ggcgcggcgg cggacgttca atccgccggg cagttcgccg tccgcaccca
     6241 agccgcgttt gatgcactct tccataatct cggcaacacc ggcaacccgg cggcggattt
     6301 cggcttcgcc gcatccggca agcgcggctt cgtttgccaa cacgacttcg gagatgtcga
     6361 gccggttcag acggcatcgg gcaagcagcc cggcacaact gttgtaagga taaggaacgg
     6421 ctttttccgt ttccgactgc cactcgaagt cttcttcggt aacgacaaag ccgccgccga
     6481 ccgaataata aacctgttcc ttcaataccg tgccgtctga agcataggcg gtaaatcgca
     6541 ggccgttggg gtgtttgggc agcacttgat tgccgcgtat gttcaggtcg cggtcgggga
     6601 tgaagcggat ttcttgcccg ttgagccgga ggatgtgctg cgtgcggatg cgtccgaggc
     6661 gttcgggaat gccggcaagc gggatgtcgt gcggcaggct gccttccaaa ccgagcatca
     6721 gcgcgtcaaa tgtgccgtgt ccgtatccgg tcagggcaag cgagccgtaa atgtcgatgg
     6781 cgatgcgggc ggtttgcgcg tccaagcctg cggcaaaggc ggcggcggcc ttcatcgggc
     6841 cgaccgtatg cgaactggaa gggccgatac cgattttgaa aatatcaaaa atgctgatca
     6901 tggttttgct ccgaaaggcg gtttcagacg gcatggcttc cgtttgacaa accaaaagga
     6961 gacgcggcaa gctgcccgtc tcctttttta aaacggcact tatgcgtcga tattttgcgc
     7021 aatcagcgca ttgttttcga taaaggcgcg gcgcggttcg acctcgtcgc ccatcagggt
     7081 aacgaacact tcgtcggcgg caatggcatc ttcgatgcgc actttcaaca ggcggcgcac
     7141 ggcgggatcc atcgtggttt cccaaagctg ctcggggttc atctcgccca aacctttgta
     7201 tcgttggatg gacatacctt tttgggcaac gctcatcaag atgtccaaag cggtttcaaa
     7261 gctgtccgcg tcgtactcgt tctcgccttt gtagagcttg gcgccctcgc cgaccaagcc
     7321 tttgagcgcg gcggcggttt gggtgagggt ttggtaggct ttgctgttga ggaacttggg
     7381 ttcgatgtag ctgaccatga cgttgccgtg cagcttgcgc gtgattttga tgaaccggtg
     7441 tccttcatga ccttcgatgc gttcgagggc ggcttctttt tcgtcaagca aaccggaaag
     7501 ttcggcaacg gctttatcgg cgttttcaga cgacgtcaaa tcaatgggcg acgcgtgcag
     7561 catggcacgc aggacgagtt cgtccacgaa gcggctttcc tgttcgatga cggttttcgc
     7621 caacaagaat tgtttggcgg tgtcggcaag ttctgcgcct tcgatggtgc ggccgtctga
     7681 aacgattttg gctttttcca aggcaaggcc gagcagccat tggtcttttt ccagttcgtc
     7741 tttgaggtaa cgctcctgct tgccgtattt ggctttgtag agcggcggct gggcgatgta
     7801 aatgtagccg cgctcgacca gttcgggcat ttggcggtag aagaaggtca ggagcagggt
     7861 gcggatgtgc gcaccgtcca cgtcggcatc ggtcatgatg atgatgcggt ggtagcgtag
     7921 tttttcaggg ttgaactctt ctttgccgat gcctgcaccc agcgcggtaa tcagggtggc
     7981 gacctcttgg ctggcgagca ttttttcaaa acgtgctttt tcgacgttca aaattttacc
     8041 tttgagcggc aaaatcgctt ggaatttgcg gtcgcggccc tgcatggcgg aaccgcctgc
     8101 ggagtcgccc tcgacgaggt agagttcaga cagggcaggg tctttttctt ggcagtcggc
     8161 gagtttgccg ggcagtccca agccgtccat cacgcctttg cggcgggtga tttcgcgggc
     8221 tttgcgggcg gcttcgcgtg cgcgggcggc atcgacgatt ttgccggtga tgattttggc
     8281 ttcgttcgga ttttcttcga ggaagtcggt tagtgcttgg ttgatgactt cgttgacaac
     8341 ggggccgatt tcgccggaaa ccagtttgtc tttggtttgg gatgagaatt tggggtcggg
     8401 cagtttgacg gacaacacgc aggtcaaacc ttcgcgcata tcgtcgccgg cggtttccac
     8461 tttggctttt ttggcgactt cgttagcttc gatgtagctg ttgatggtgc gcgtcatcac
     8521 ttggcgcagc gcggtcaggt gcgtaccgcc gtcgcgctga gggatgttgt tggtgaagca
     8581 ctgcacgctt tcctgatagc tgtcgttcca ttgcattgcg cattcgacgc tcatgccgtc
     8641 tttctcgccg aacgcataga agattttttc gtgcaagggc gtttttttgc ggttcatgta
     8701 ttgcacgaag cccgccacgc cgccggaaag ggcgaagctt tcgtgcttgc cgtcgcgctc
     8761 gtcggtcaat tcgatgtcca cgccgttgtt taggaacgaa agttcgcgaa tacgtttggc
     8821 gaggatgtcg aagctgtatt cgatattgcc gaaggtttcc gtgccggcga ggaagcgcac
     8881 ggtcgtgcct tttttgtcgg aatcgccgac aattttcagc ggctcttcgg tttcgccgcg
     8941 tacgaagcgg acgaagtgtt ctttgccgtc gcggtagatg gtcagcgtta cccagtcgga
     9001 cagcgcgttg acgacggata cgcccacgcc gtgcaggccg ccggagattt tgtagctgtt
     9061 gttgtcgaat ttgccgcccg cgtgcaagac ggtcatgatg acttcggcgg cggaacgccc
     9121 ttctttcggg tggatgccgg tgggcatacc gcgcccgttg tcggcgacgc tgacggaatg
     9181 gtcggcgtgt atcgttaccg tgattttgtc gcaatgtccg gcgagtgctt cgtcgatggc
     9241 gttgtccaat acttcaaaca ccatatggtg cagcccgctg ccgtcctgcg tgtcgccgat
     9301 gtacatgccg gggcgtttgc gtaccgcttc caagccttcg agcacctgga tgctgtcggc
     9361 gccgtattct tcgtgttttt gttcagtcat attttttgcc ggattttgaa aagattttgc
     9421 gatgccgcca aaacagtccg caccttgtgg aaaaagcggg cgggacggcg catataattg
     9481 tgtattatag ccgatttcgc cgcctgattc agcgttatcc gcatccggat gccgtctgaa
     9541 agggcgtgcg gcgcatttgc ggccggtacg ggcgcggaat ttgccggcaa agtggcaaaa
     9601 gcgttttttt gccactaaaa tctacaccct atacttttcg gacaggggcg cggaaatgga
     9661 aatatggaat atgttgaaca cttggcccga tgccgtcccg atacgcgcgg aggcggccga
     9721 atccgtggcg gcggtcgcgg ctttgctgct ggcgcgcgcc cttctgttga atatccactt
     9781 cagacggcat ccggatttcg gcatcgaaag caagcggcgg tttttggttg ccagccgcaa
     9841 tataacgctg cttttggtgc tgttttcgct ggcatttatc tggtcggcgc aaattcaaac
     9901 gctggctttg tcgatgtttg cggtggcggc ggcggtcgtc gtggcgacaa aagaactgat
     9961 tatgtgtctg tcgggcagta ttttaaggtc tgccacccag caatactcgg tcggcgacta
    10021 tatcgaaatc aacggcctgc gcgggcgcgt ggtcgacatc aatctgttga acacgctgat
    10081 gatgcaggtc ggtccgaacc ccttggtcgg acagcttgcg ggaaccaccg tttctttccc
    10141 caacagcctg ttgttgagcc accccgtgcg ccgcgacaat attttgggcg actatgtcat
    10201 ccatacggtc gaaatccccg ttcccatcca tttggattcg gatgaagccg tatgccgtct
    10261 gaaagccgta ctcgagccct tgtgcgcgcc ctacatcccc gccattcagc ggtatttgga
    10321 aaacgtgcag gcggaaaaac tgtttatcac gcccgccgcc aggccgcgcg ttacccgcgt
    10381 accgtacgac gacaaggcat accgcatcat cgtccgcttc gcctcccccg tttcaaagcg
    10441 gctggaaatc caacaggcgg ttatggacga atttttgcgc gtacaatacc gcctgttaaa
    10501 tcatcccgcc ggctccgaaa cactttaact ttccccggcc ggccccattt ctggcttcag
    10561 acggcatatt gccgatatgc cgtctgaagc acaacacgca aaggaaaccc atcttatgat
    10621 tgacaacgca ctgctccact tgggcgaaga accccgtttt aatcaaatca aaaccgaaga
    10681 catcaaaccc gccgtccaaa ccgccatcgc cgaagcgcgc ggacaaatcg ccgccgtcaa
    10741 agcgcaaacg cacaccggct gggcgaacac cgtcgagcgt ctgaccggca tcaccgaacg
    10801 cgtcggcagg atttggggcg tcgtgtccca tctcaactcc gtcgtcgaca cgcccgaact
    10861 gcgcgccgtc tataacgaac tgatgcctga aatcaccgtc ttcttcaccg aaatcggaca
    10921 agacatcgaa ctgtacaacc gcttcaaaac catcaaaaat tcccccgaat ttgcaacgct
    10981 ttcccccgca caaaaaacca agctcgatca cgacctgcgc gatttcgtat tgagcggcgc
    11041 ggaactgccg cccgaacggc aggcagaact ggcaaaactg caaaccgaag gcgcgcaact
    11101 ttccgccaaa ttctcccaaa acgtcctaga cgcgaccgac gcgttcggca tttactttga
    11161 cgatgccgca ccgcttgccg gcattcccga agacgcgctc gcgatgtttg ccgccgccgc
    11221 gcaaagcgaa ggcaaaacag gttacaaaat cggcttgcag attccgcact accttgccgt
    11281 catccaatac gccggcaacc gcgaactgcg cgaacaaatc taccgcgcct acgttacccg
    11341 tgccagcgaa ctttcaaacg acggcaaatt cgacaacacc gccaacatcg accgcacgct
    11401 cgaaaacgca ttgaaaaccg ccaaactgct cggctttaaa aattacgccg aattgtcgct
    11461 ggcaaccaaa atggcggaca cgcccgaaca ggttttaaac ttcctgcacg acctcgcccg
    11521 ccgcgccaaa ccctacgccg aaaaagacct cgccgaagtc aaagccttcg cccgcgaaca
    11581 cctcggtctc gccgacccgc agccgtggga cttgagctac gccggcgaaa aactgcgcga
    11641 agccaaatac gcattcagcg aaaccgaagt caaaaaatac ttccccgtcg gcaaagtatt
    11701 aaacggactg ttcgcccaaa tcaaaaaact ctacggcatc ggatttgccg aaaaaaccgt
    11761 ccccgtctgg cacaaagacg tgcgctactt cgagctggag caaaacggca aaaccatcgg
    11821 cggcgtttat atggatttgt acgcacgcga aggcaaacgc ggcggcgcgt ggatgaacga
    11881 ctacaaaggc cgccgccgct ttgccgacgg cacgctgcaa ctgcccaccg cctacctcgt
    11941 ctgcaacttc gccccgcccg tcggcggcaa agaagcgcgt ttaagccacg acgaaatcct
    12001 caccctcttc cacgaaaccg gccacggact gcaccacctg cttacccaag tggacgaact
    12061 gggcgtgtcc ggcatcaacg gcgtagaatg ggacgcggtc gaactgccca gccagtttat
    12121 ggaaaacttc gtttgggaat acaatgtatt ggcacaaatg tccgcccacg aagaaaccgg
    12181 cgagcccctg ccgaaagaac tcttcgacaa aatgctcgcc gccaaaaact tccaacgcgg
    12241 catgttcctc gtccgccaaa tggaattcgc cctctttgac atgaccattt acagcgaaga
    12301 caacgaaggc cgtctgaaaa actggcagca ggttttagac agcgtgcgca aagaagtcgc
    12361 cgtcatccaa ccgcccgaat acaaccgctt cgccaacagc ttcggccaca tcttcgccgg
    12421 cggctactcc gcaggctatt acagctacgc atgggcggaa gtcctcagcg ccgacgccta
    12481 cgccgccttt gaagaaagcg acgacgttgc cgccacaggc aaacgcttct ggcaggaaat
    12541 cctcgccgtc ggcggctccc gcagcgcggc ggaatccttc aaggccttcc gcggacgcga
    12601 accgagcata gacgcactgc tgcgccacag cggcttcgac aacgcggctt gatagtcagg
    12661 ttgatataaa aagcggattc gatgtagaaa tacctgaatc gttatccccg cgcaggcggg
    12721 aatccggaac ataaaattga agaaaccgtt ttacccgata agtttctgtg ccgacaagtc
    12781 tggattcccg cctgcgtggg aatgacgaca aagatatttt tatttgaaat ttactataaa
    12841 aggccgtctg aaaatttttc agacggcctt tttataatct aaaactgtcg gattcgggaa
    12901 tacaatccaa acaggctatt gattatcata atgccgaagt acaaaccccc aaagccgatg
    12961 gtaatggtag cgcaagaata ttgattaatt atccaaacgc atcatggctt acacaacgca
    13021 gccattgaat taggcttcag cccaccaatc cctccgccta cgccgcgcat tcgtgaaaaa
    13081 cgcataagac ccggacatgg ttggttgaat tggtggaaac ggctggctga agcacaccct
    13141 acggcatacc ctgcgtatct gacgcgacga aatataaaat gccgtctgaa acagattccc
    13201 gtttcagacg gcatttttgc gtaccggccg gattatttgt tgtccggctt agattccatc
    13261 ggtgcggtca ggggcagttc cggcatcgtg cgcgctgatt cggaaacatt gccggaaagt
    13321 gcattcagga atacgacgat gttatccaca tcttctttcg gaatgtcttt acccaattgc
    13381 gccttaccca tgatggtaac tgccttatcc agctcccaca cgctgccgtt gtggaaatac
    13441 ggataagttt tagccacgtt acgcaacccc ggaacacgga agaaaaattc gtcttcggtt
    13501 tttttggtta cgtcagcacg gcctttgtcg cgtttcggat cttcaatgaa tttccaatac
    13561 ggcccttgga ccagaccgaa tttctggaag gtcgtgcctc caaggttgac accgttgtgg
    13621 caggcaatac agccgttgtc catgaacgcg cgcacgcctt ttcgttcctg ttcgctcagg
    13681 gcgttgacgt tgcctttgag gtattcgtcc catttggtcg gcgtcagcag ggtacgctca
    13741 aacgcaccca atgcggtagt gatgttttta aacgaaaccg cgccgtcttc aggaaaagct
    13801 tttttaaaca tttcttgata ttcgggaact ttggcgattt tggctgcagc cgcctcttgc
    13861 gaatcattcg ccatttccac cggattcacc aaaggcccgc cagcctgttc ttcaacatcg
    13921 gcggcacgtc cgtcccaaaa ctggctgccc agcagcgcag cattcaatgc ggtaggcgag
    13981 ttgcgtccgc cgaactgccc tttgtgcccc tgactggtcg gcatattgtc cacaccggcg
    14041 gaagcaaggt tgtggcaaga gttgcagctt acggtattgc ctttggaaag gcgtggttca
    14101 taccagagtt ggtgtccgag tttgacctgt tcttcggtaa acggacggat tttctgcatt
    14161 tcttcgacag tcggcaaagg ttggaataca ccttgcgcgc gtttcaaaag gtcttggtct
    14221 tcgggcgagg ccgatgaagc ggaaggaacg gcttccgagg cctgtacttg ggaagccgct
    14281 tcggtctcgg aagcaggaga agcatcgcct gccgcagact tttcctgacc gccgcatgcc
    14341 gccaacagtg aagacaatgc caatactgat gcgaggtaac ggagtttgaa agacatcgtg
    14401 tattccttat gggaggttaa aattattgtt caaaccggca ggcaaacctg ttctgccgat
    14461 tatttcttca gtgtacctat cgcaccttta gccatctttg cggaaaatca atattttatt
    14521 ttcaatagct ttacacaata ataccgatag agaatcaagt ttaacaaaat gccgtctgaa
    14581 accggtaaag cttcagacgg cattttattt tccgacatca ttgaatcaaa cccaaatgcg
    14641 ataagagcgt ccatgtaccg atggcaatca acaccaaacc tccggcaaat tccgcacgcc
    14701 tgccgaacaa tacgccaaaa gcctttcccg ccgtcagccc gatggtaacc attaccgtag
    14761 ccgccatacc gattacggcg gcggcaaagg cgatgtttac ctccataaac gccaagccca
    14821 ccccgacgat catggaatca atactggttc caaaagcagt caaaaccgtc atccataggc
    14881 tttcctgttt gctttcgcgc acatcttccg cctcgccgga caacccttcg cgcatcattt
    14941 tcagacccag tccgcccaac aggacaaaag ccacccaatg gtctcattcg ctgataaacg
    15001 gcttggcata aaaaccgcct acccagcccg ccagcggcat gaacgcttca accgtaccga
    15061 acaccaaagc cgttgctgca attttgcgcg ggggcatcct gaccgccgca ccctttgcca
    15121 gcgcgacggc aaacgcatcc atcgacattc ccagcgcaac caagagcaaa gcgtaaagac
    15181 tcataccgca cccatcctta aaaagggcgg attataacaa aacagcaaag cgcgaaaaac
    15241 gccgcacgaa aacccgcatc ccgttattcc cgcaaaaaca aaaaatcaaa aacagaaatc
    15301 ccgtcattcc cacgaaagtg ggaatccagc cccgtcggca cggaaactta tcggataaaa
    15361 cggtttcttt agattttacg ttccggattc ccactttcgc gggaatgacg cggaaaagtt
    15421 ttcgtgccgg tttgaaccgg tggggatggt gcgggaatga cgggatattt tgcgtttata
    15481 gtggattaac aaaaatcagg acaaggcgag gcaacgccgt accggtttaa atttaatcca
    15541 ctataataaa aaacgcccac tgaaacggcg gggcgaggtg tggaaatgcc gtctgaaacg
    15601 gtcaaacgac gtttcagacg gcatttttat gcccggttat ttccggtatc ggacggcgcg
    15661 ggacaggatt tcttcaattt ccatccacat aatgccccct tacagcaaac cggcctgacc
    15721 cagtgcggga tcggtcgcgc gggcggcttg tgcgtcttcg acggtcagtc cgagggcttt
    15781 ggctacgcct tcaccgtatg ccgggtcgca acggtagcag ttgcggatat ggcggtattt
    15841 gatgaagtcg ggcgcatcgc ccatggctgc ggcggtgttg tcaaacaaag cctgtttctg
    15901 cgcgtcgttc atcaggttga acaaggcgcg cggttggctg aagtagtcgt catcgtcttg
    15961 gcggtagtcc cagtgtgccg cgtcgccgtt gattttcaaa ggcggttcgg cgaagtcggg
    16021 ttgttgctgc cattggccga agctgttggg ttcgtagtgc ggcaggctgc cgtagttgcc
    16081 gtcggcgcgg ccttgtccgt cgcgctggtt gctgtgaaca gggcaacgcg gacgattgac
    16141 ggggatttgg cggaagttca cacccaagcg gtaacgttgc gcgtcggcgt aattgaacaa
    16201 acgggcttgc aacattttat ccgggctcgc gccgataccg ggaacgaggt tgctcggtgc
    16261 gaaggcggat tgttccacat cggcgaagaa gttttcggga ttgcggttca actcgaattc
    16321 gcccacttca atcagcggat agtctttttt cggccaaact ttggtcaagt caaacggatg
    16381 ataaggcact ttttcggcat cggcttcagg catgacttgg atgtacatcg tccatttcgg
    16441 gaactcgccg cgctcgatgg cttcgtacag gtcgcgctga tggctttcgc ggtcgtcggc
    16501 gatgattttt gcagcttctt cgttggtcag gtttttaatc ccttgctggc tgcggaaatg
    16561 gaatttcacc caaaaacgtt cgcccgcttc gttccagaag ctgtaggtat gcgaaccgaa
    16621 gccgtgcata tggcggtagc tggcgggaat accgcggtcg ctcatcacga tggtaacttg
    16681 gtgcagggct tcgggcagca gcgtccagaa gtcccagttg tttgtggcgg aacgcatatt
    16741 ggtgcgcgga tcgcgtttga cggctttgtt caggtcgggg aatttgcgcg ggtcgcgcag
    16801 gaagaacacg ggcgtgttgt tgccgaccac atcccagttg ccttcttcgg tatagaattt
    16861 caacgcaaaa ccgcggatgt cgcgttccgc atcggctgcg ccgcgctcgc ctgccacggt
    16921 ggtgaaacgg gcgaacatct cggttttttt gccgacttcg ctgaaaattt tggcgcgggt
    16981 gtatttggtg atgtcgtgtg ttacggtaaa cgtaccgaac gcgcccgaac ctttggcgtg
    17041 catacggcgt tcggggatga cttcgcgcac gaagtcggcg agtttttcat tcagccacaa
    17101 atcttgcgtc agcagggggc cgcgcgggcc ggcggtcagg ctgttttgat tgtcggcaac
    17161 gggcgcgccg ttgttcatgg tcagatgggt tacggggcat ttggaggtag tcatcgctct
    17221 tgttcctttt ctcaggttgg tcaaatgggg ggcaaacggc ttacagtacg atttggcgga
    17281 aagcgtattc gtaaccggtt tcttgattgt aataaatttc ttgaatcgac attttatttt
    17341 ccttttgcaa aaactatgga tgcgattata cgccaagatt ttcgttatta aaactatgaa
    17401 attgatttaa tattgttata agcaatcaag tttccatttt cgtttgtttt ttgttatcgg
    17461 acggaatccg aacccgctca ttaaaaccat ttataatgca atgacgcttt gcggcatttt
    17521 tttgcgccga caggctgaaa ataacaattt ttaccgcatt atcattacct taatcggaat
    17581 aaagctcaaa cagacccagc agctcgacca gcggctgcaa caatctttgc gcgtattgca
    17641 gatgccgggt atcgaacttg aacgcgaggt cgaaaactgg ccgtcggaca accccctgct
    17701 cgaacgcaaa gaaacggatg aattttccga tgccgaattc agccattaca ccgcgcccgc
    17761 gcgtcaaatc ggcggagacg aaggcgaaga tatgctgtcc aacatcgccg gcgaagaaga
    17821 tttcaagcaa tacctgcacg cgcaagcgtg cgaacacccc ctttccgacc aagaagccgc
    17881 ctgcgtccac atccttatcg atttccttga cgagcagggc tacctgaccg acagcatcga
    17941 agacatcctg gaccatacgc ccttggagtg gatgttggat gaagcaatgc tgaaacaagc
    18001 cctgaccgca ttgaaaaaat tcgatccggc aggcatggcc gccgccgatg tgaccgaatc
    18061 gttgatattg cagatagaaa gatcgggcga atgtgctgcc aaacccgccg ccctgcatat
    18121 cgtccgaaac gccctcgaca gcattgacgg caaccgcagc caaaccccgg cgcgaataaa
    18181 aaacgcctgc cccaaaccga cagcggcaca ctcgaagccg cactcggcct cattgcttcg
    18241 ctcaacccct ttcccgccgc cggttttgcc tcgtccacgc ccaagtccta ttctgacgag
    18301 gcactcgcca acctgctggc tttccgcggt atagaggttt cccgccgcac cgttgccaaa
    18361 tacagagaat cccttgagat tccggcagca cacaaacgca aaaccgcaga ataattaccg
    18421 aataatctta taaagacaac aaaccaaaag ccgacatttc tgcgaaagcg ggaatgccga
    18481 atccgtccgc gcggaaacct gcatcccgtc attcccgcga aagtgggaat ctagaacgta
    18541 aaatctgaag aaactttttt tcgataagtt tctgtaccgg cggggctgga ttcccgcctg
    18601 cgcgagaatg acgggatata agttgctgtg ccggtttgag ccggtgaggt tggtggcggg
    18661 attggtttcg gtgggctgaa gcccacccta cagcccgccc tacacatctg aaactcaacg
    18721 aacctggatt cccgctttcg cgggaatgac ggggtttcgc gggaatggcg ggagtttgtc
    18781 agaaatcacc gaaactcaaa agccgaccac cttgtttacg ccttcaaaat atcgagaaat
    18841 ttcaaatcga ctttttcggc atcgaattta tctttggcaa tcgcataact tgcattcccc
    18901 atcaggcgga cggcagccct gttttcgata aaataaatca ttttttcggc caagatgcgg
    18961 ggattccaag gttcgatcag gaagccgttg accttgtcgg caaccgtttc cctgcatccg
    19021 gggacatccg tcgtaatcac cgccctgccg acggccattg cctcctgagt gctccgggga
    19081 acgccttccc tataataaga cggcaatacg aatatatgat gttcttttat catttcggaa
    19141 acattgttca caaaaccggg gaaacggata atatcgcggg cggcaggctg ttccaaatcc
    19201 ccgctcccgc gtggtttgtc gattgcgccc aaagcggtaa aaaccgtatc ggggtatttg
    19261 cccttaacct gttccgccgc ccgaataaac tcatcaatcc ctttttcttt cagaaatctg
    19321 ccgataaaga ggaattttac gggttctttt tcatcgggaa tatccgcctc ggaataagga
    19381 tattgccgca aatccagacc gattccgccc aaaatatgga tgttttttat tttgatgccg
    19441 tattgatgca gcaactcgtc tttgtcgtcg gggtttaata caatcaggct ttccaacatc
    19501 ggcagggtaa tgcggtacaa ggcgattaaa atccccttta tgatttttgt ttttaacggt
    19561 atgccttccg gctgcggggt aaatgcgaat cccaaacctt ccagcatccc gacgattctg
    19621 ggcacgcctg ccaattttgc ggcaaaagtg ccgaaaatca cgggttttgc gaaataaggg
    19681 acaaccaaat ccggcgatat ttttttgagt gctttaaata tgaggaaggt ggattttata
    19741 tcggaaaacg ggtttagccc gctgcggttt gaccggtagg taacgggtgt aacccccatt
    19801 tccctgataa tgtccaactc attgtcggaa aactccgata caaaggcata cacctgatgg
    19861 tttttgccga ttaatttttt aatgacgggg gcgcggaaac cgtaaatgct ggatgcgact
    19921 gttgtgataa aaacgatttt cataaggcgg acaccttgaa tataggattg gaaatgcggt
    19981 ctgcattgat gaaatgccgg atacggggcg cggcgggcaa acctttgcca agcgcgccgc
    20041 atccgacgct tcgcccgccc gggcaggggc ggcttcagac ggcattttat cctgaaatcc
    20101 gctgcataac gcccagcgat atacagtatc gcgttttgtg atgattttac acccgaaggg
    20161 aaaacgggct tatttgaatg gcggtttaat cgcggcggtt atttgcggtt tgataaggct
    20221 gatttgcgtg ccgtgcagtc cggcaacggg cggggtgttt ggaaaatgcc gtctgaaggt
    20281 ttcagacggc attctgtcat gcggcggttc ggggttcgcg gacgggcagg acaagcaggg
    20341 ctgccgtgcc ggcgagtacg gcatcggcgt accacatcca gccgtagtca ccgaattgtg
    20401 taatcacgat gccgccgatg tacgagccga ggaatccgcc gatttggtgg gtgagcatca
    20461 ccagtccgaa cagggtggcg aggtagcgcg tgccgaagag tttgccggta acggcggcgg
    20521 tcggggcgac ggtggcgagc catgtgaatc cgagtgcggc ggcgaaaatg taaaagttga
    20581 ggtcggtttt gggtgagaag atgtaaatca gcaccatggc gacgcgtgag gcgtagagtc
    20641 cgaacaggat gtgttttcct ttgaagcgtc cggtgcatca gcctgagaac acgcaaccgg
    20701 cgatgtttgc cagtccgatg atggcgatgg atgtcgaggc gacggtggcg ggcagtccgc
    20761 acagggcgac ttccgtgggt aggtgggtta cgagaaaggc gatgtggaag ccgcaggcga
    20821 aaaagctcag gtgcagcagg atgtagcttg gggttttgaa ggcggttttg actgcttctc
    20881 cgaggctttg tccgtgtgtg gcttgggtgt gttgggtgtg ggcggcgttg ttgccgccgg
    20941 caagccacca tgagacgggc agaatcagca gggcgattgc gccccaaacg taaaatgtac
    21001 ccgtccagcc gacttcgggc aggacgacga gtccttgagt ccttgaacca gcggtgcgaa
    21061 caggaattgt cctgccgaac cgcctgcgtt gaccagtccg gatgccaagc cgcgtttgtg
    21121 tgtggggact tgggcggcga cctgtcccat aatgatggaa aacccgccgg aaccggtgcc
    21181 gaatgcgagc agcagcccga cggcaatcat cagcccccaa taagtcggga tgttggaggc
    21241 aatcaggcag gcgcagacga gcaggagggc accgccgctt aataccctga acgcgccgaa
    21301 acggtcggca agcgcgccgg acaatggttg caatacgccc cacatcagtt ggaaaacggc
    21361 gatgatgagg ctgaactggg caatgttcaa ttcggtggtg ttgacgacgg gttggacgaa
    21421 cagtccgagc gtcatcctca tgccgatggt aatcagcagg atgaatgcgg cggcggcaat
    21481 gaccgcccag agtttgggtg ttttcgatgc ggtgtgcgtc ataatatgta ttttgagtcg
    21541 gttaagtttg cgaattttaa caaaccggat gattcgggct aaataattcc tgattctttg
    21601 gatatagcca acggtatgtc tgccgatgcc gtctgaaagg gcttgatggg cgtttgcggt
    21661 tcgggttaat atttgtgccg tatttgttaa ttccagttag tatagtggat taactttaaa
    21721 ccggtacggc gttgccccgc cttagctcaa agagaacgat tccctaaggt gctgaagcac
    21781 cgggcgaacc ggttccgtac catttgtacc gtctgcggcc cgccgccttg tcctgatttt
    21841 tgttaatcca ctataaaaat gccgtctgaa acggtttcag acggcatttc gatgtcggcg
    21901 gcggctttgc ggaatcagcc tttgaagcgt ttgaagacca gcgtaccgtt ggtgccgccg
    21961 aagccgaagg agttggaaat ggcaacgtcg atttccgcgt cgcgcgcttc gttggcgcag
    22021 tagtccaaat cgcagccggc ttcaacgtct tgttcaaaaa tgttgatggt cggcgggatt
    22081 ttgccgtcgt gtatcgccaa aatgctgtac acggcctcca cgccgcccgc cgcgccgagc
    22141 aggtggccgg tcatggattt ggtcgagctg atgacggttt tgcaggcgtg ttcgccgaac
    22201 gcgcgtttga gggctttggt ttcgttggca tcgcccaagg gggtggacgt gccgtgcgcg
    22261 ttgacgtaat ccacgtcttc gggattgatg ccggcatctt tcagcgcgcg ggtaacggca
    22321 agggcggggc cttcttcgtt cggcgcggtg atatggtaag catcggaact catgccgaag
    22381 ccgacgattt cggcgtagat tttcgcgccg cgttttttgg cgtgttctaa ttcttccaac
    22441 accaatatgc ccgcgccttc gccgatgacg aagccgtcgc ggcctttgtc ccacggacgg
    22501 gaagcggtgg cggggtcgtc gttgcgggtg gagagggctt tcatcgcagc aaaaccgccc
    22561 acgcccaagg tgctgattgc gccttccgcg ccgccggcaa ccattatgtc cgcgtcgccg
    22621 tatttgatca gtcgggcgga atcgccgatg gcgtgcgcgc cggtggtaca ggcggaaacc
    22681 atgccgtagc tcgggccgcg gtagcctttg aggatggtaa cgtgtccgga aatcaggttg
    22741 atcagcgaac cggggataaa gaaagggttg attttgcgcg cgccgccttc gattacggct
    22801 ttgccggtgg cctcgatgct gggcagtccg ccgatgccgg aaccgatgtt cacgccgatg
    22861 cggtctttgt cgaggttttc cacatcgtcc aaacccgaat cggcaatcgc ctgcaatgcg
    22921 gcggcgatgc cgtagtggat gaacacgtcc atccggcgcg cttctttcgc gctgatgtat
    22981 tgtccgatgt cgaaaccgcg cacttcgccg gcgacacggc tgttgatgtc ggatgcgtca
    23041 aagcgggtaa tcgcgccgat gccgcttttg ccggcgagca ggttgtccca agcctctgcg
    23101 gcagtgttgc cgacagggga aacctgacct aagcctgtaa tgactactct tctctgactc
    23161 atgataacct cgctgttggt tgtcggaatg gggggcatat gcggctgtcg tgcagatgcc
    23221 gtctgtaatt tgcggcaggg gttcaaacag tttgccatat aagggaaaag cctctattgc
    23281 gcggtgcagc agaggctgtt gtgtcgggcg acgaccggtt agccgttgtg ggcattgatg
    23341 tagtcgatag ccagttggac ggtggtgatt ttttcggcat cttcgtcggg gatttcgcag
    23401 ccgaaggctt cttccaaagc cataaccagc tccacggtat ccaaagaatc cgcgcccaag
    23461 tcgtcttgga aggaagattc gtttttcacg tcggcttcgt ttacgcccag ttgttcagca
    23521 ataattttct taacttgttg ttcgatgttt gacatatcag tcgttccttt atgccttgcg
    23581 gcaggttgtt taagggaaat aaatcggtgg tattgtaccg atttttaata gagttttcta
    23641 tctaatgcct attatatcaa tatttgcgga tttgtacatt tttgggtgcg gcgggttttg
    23701 tcgttcaagt ttgacctgtg tgccgtatgt ttggcgggat ttcggttaaa atggcggcat
    23761 ttccatctga agcagaaagc cctgtcatgt atccacttgc ccgccgcatc ctgtttgcac
    23821 tcgatgccga aaaagcccac cacttcacgc tcgacgcgct caacacggtt tataaattgg
    23881 gcctgattcc cgtaaccgac aaccgtacca aacctataaa actgatgggt atggatttgc
    23941 ccaaccctgt cgggcttgcc gcaggtctcg acaaaaacgg tgaatacatc gacgctttgg
    24001 gcgcgctcgg tttcggcttc ctcgaaatcg gcacggttac gcgcaacccg cagcccggca
    24061 acccgcagcc gcgcctcttc cgcgttcccg aacaccaagg catcatcaac cgaatgggtt
    24121 ttaacaacca cggcatcgac gcgatgatac gcaatattga aaaaagcaaa tatcaaggcg
    24181 tattgggcat caacatcggc aaaaacgcgg ttacgcccat ccaaaacgcc gccgatgatt
    24241 atttaatctg cctcgaaaaa gcctacgcgc acgcaagcta cattaccgtc aacatttcct
    24301 cgcccaacac caaaaacctc cgcgcgctgc aaggcggcgg cgaattgggc gcattgctcg
    24361 aagccctgaa aaacaaacag gcgcagcttg ccgccgccca cggcaaatac atcccgctag
    24421 ccgtcaaaat cgcccccgat ttggatgaag cacaaatcga agacatcgcc cgcgtcgtca
    24481 aatccgtcga aatggacggc atcatcgcta ccaacaccac catcgacaaa tcaagcctcg
    24541 gcagccatcc gctcgcaggc gagcagggcg gtttgagcgg ttttcccgtg cgcgaaaaaa
    24601 gcaaccgggt gctgaaaaaa ctggcggaac acatagacgg cgcattgccg attatcggcg
    24661 taggcggcat tatggaaggc ggggacgcgg cagagaaaat ccgcttgggc acgaccgccg
    24721 tccaagtgta cagcggattg atatacaaag gcccggcgtt ggtcaaagaa tgtttgaagg
    24781 cattggcgcg atgacgcgcc ccacccaaaa tgccgtctga acgcacgttt tgccgttcag
    24841 acggcatttt catagattgg tatccgactg ttgaagaggc aatcagccgt catttggaag
    24901 acttttcgta acaggcgtca atcgaaaggg gcggaatgac ggggcaggca aaccgatgcg
    24961 tgaacacggg gcaggcaacc ggagtaaaaa atgaaccttg atttaaccgc tcaaaaagtc
    25021 cgcctttctt ggaaggatat tctgtggggg tatgagaata aatacttggg ttgggctgat
    25081 gtggcagctt atgcccgaaa aatgacgctt tcagatcatg atgaacgcgt gttcaagcta
    25141 tctttaacca acaaatccaa tatttttgaa ttaaagcctg ttctagaaga tttggcttcg
    25201 gaaacgaggg gttattcccc taaaaattgg ctgtacatcc tcctaaatga cgtattccat
    25261 agaaaggaag aatttgagga tcctctgggg gaagttgaaa aaatttatgc ggattttgat
    25321 tacccggaag aaatagaatc atttgtcagg tatatgccgc ccaaagacgg ctatatccct
    25381 tctaatcata gttatgaaga aaatattgcc cggttatatt ctcactggga acactatttg
    25441 aacaacggcg gcgggcaggg ttaaaaccgg caatccgatg ccgtctgaag cgttatccgg
    25501 cgttcagacg gcattttgtt ttccgacagt ttataaactg tcgttgtttc ttgacagaaa
    25561 caacgacctt atttatttga aacgattgga ggacatgatt atgggttttt ggggtagtgt
    25621 gggaaaagca gcaaaagcag taggcgaggg attgattgaa gccggcaatc agcataaggc
    25681 gttgaaaatg gaatatgcgg agaaatcaag tgaggagctg catgaaatcg taaagagtga
    25741 tggttttttt aaaaattcca cacgggagaa aggtgcggct tatgctgttt taaaagagcg
    25801 tggcgaggtt tgaacgggaa acggcggcat ttgccgctgt ttttttatcg gcaggcatcc
    25861 gtccgaatat cggggcaagg tttcagacgg catcgaaggt tgctatgata tagtggcttg
    25921 actttaaacc ggtacggctc cgccttgccc tgatttgaag ttcatccgct atcacattcc
    25981 cgccatcctt tcaaacggaa tccgaaatgt ccgaccaccg cctcgacacc gcccgccgcc
    26041 attccctgtt cctcgcccgc cagctcgaca acggcaaact caagcccgaa attttcctgc
    26101 ccatgctgga caaggcgttg acggatgagg atttccaagc ctttgccgac tgggacaaaa
    26161 tccgcgcgga agaaagcgag gaagaattgg cgcggcagtt gcgcgagttg cgccgttatg
    26221 tggtgtcgca gattatcgtg cgcgatataa accgcatcag cgatttgaac gaagttaccc
    26281 gcacgattac gctgtttgcc gattttgccg tcaataccgc gctggatttc gcctacgcct
    26341 attatcagga catgtacggc acgccgatcg ggcgttatac caaatcgccg cagcatttga
    26401 gcgtggtggc gatgggcaag gcgggcggct atgagctgaa cgtgtcttcc gacatcgatt
    26461 tgattttcgt ctatcccgaa tcgggcgaca ccgacggcag gcgcgaacgg gggaatcagg
    26521 agttttttac caaagtcggg cagaaactga ttgcattgtt gaacggcatt actgccgacg
    26581 ggcaggtgtt ccgcgtcgat atgcggctgc ggccggacgg tgattcgggc gcgctggtgt
    26641 tgagtgaaac cgcgctggag caatatctga ttacgcaggg gcgcgaatgg gaacgctatg
    26701 cgtggtgcaa aggccgcgta gtcacgccgt atccgaacga catcaaatcg ctggtgcgcc
    26761 ccttcgtgtt ccgcaaatat ctggattacg gcgcgtatga ggcgatgcgc aacctgcacc
    26821 gccaaatccg cagcgaagtc agcaaaaaag gcatggcgga caacatcaaa ctcggcgcgg
    26881 gcggcatccg cgaggttgaa tttatcgccc agattttcca gatgatacgc ggcggccaaa
    26941 tgcgcgcgct gcaactgaaa ggcacgcagg aaacgctgaa gaaaattgcc gagatgggca
    27001 ttatgccgtc tgaaaacgtc gaaaccctgc ttgccgccta ccgcttcctg cgcgatgtcg
    27061 aacaccgcct gcaatactgg gacgaccggc aaacccaaac cctgccgatc tcgcccgaac
    27121 agcggcaact gctcgccgaa agcatgggtt tcgacagtta tgccgctttt tcagacggcc
    27181 tcaatgttca tcggaacaaa gtcaatcagt tgttcaacga aattttgagc gaacctgaag
    27241 aacaagcgca aagcaatagc gaatggcaat gggcatggca ggaaaaaccc gacgaagaag
    27301 aacggctagg ccgtctgaaa gaacacgggt tcgatgccga agccgtcacc gcaaggctcg
    27361 aacaaatctg ccacggacat aaataccgcc gcctttccgc acacgcccag ccgcgctttg
    27421 acaccattgt gccgctgttc gtacaggcgg cggcagagca aaacaacccg acagatacgc
    27481 tgatgcggct gttggatttt ctcgaaaaca tcagcctccg ctccgcctat ctcgccttcc
    27541 tcaacgaaca tccgcaaacc ttggcacaac tggcgcagat tatgagccaa agctcgtggg
    27601 tggcggcgta tctgaacaaa tatccgattt tgttggacga actcatcagc gcgcagcttt
    27661 tggataccgc gtttgattgg caggcactcg ccgccgccct ttcagacggc atcgaagcct
    27721 gcggcggcga taccgaagcg caaatggaca cgctgcgcca tttccaacac gcccaagtct
    27781 tccgcctcgc cgtccaagac ctcgccggat tgtggacggt agaatccctc tctgaccaac
    27841 tctccgccct cgccgacacc gtcattgccg ccgccctttc gtgcgcgtgg gcggatatgc
    27901 ccaaaaaaca ccgcgacacc ccgcaattcg ccgtcatcgg ctacggcaag ctcggcggca
    27961 aagaactcgg ctacgcctcc gacctcgact tagtctatct ttacgacgat ccccatcccg
    28021 aagcaggcga cgtgtacagc cgcctcgccc gccgcctgac caaccggctt tccgccgcca
    28081 ccggcgcggg cagcctctac gaaaccgacc tgcgcctgcg ccctaatggc gacgccggtt
    28141 tcctcgcgca cagcatcgcc gcctttggaa aataccagcg tgaaaacgca tggacatggg
    28201 aacaccagtc cctcacccgc gcccgcttca tctgcggcac acccgaaatt cagacggcat
    28261 tcgaccgcat ccgcaccgaa atgctgactg ccgaacgcga ccaaaccgcc ttggcaggcg
    28321 aaatcatcga aatgcgcgaa aaaatgttcc ccacccatcc gcctgccgac agcaacgtca
    28381 aatacgcgcg cggcggcgtg gtcgacgtcg aatttatcgt ccaatatctg atacttgccc
    28441 atgcccgcca gtatccgcaa ctcttggaca actacggcaa catcgcccta ttgaacatcg
    28501 ccgccgactg cggtttgatt gacaaaaccc tcgccggaca aagccgcacc gcctaccgcc
    28561 tctaccgccg gcagcagcac aacaccaaat tgcgcgacgc ggcaaaaacc gaagtaagcg
    28621 acgaattgct gtcccattac ggcaatgtca ggaaattgtg gcgggaagtg ttcggggagg
    28681 aagcggcaac cgcctgaaca aaaaatgccg tctgaagcct gacaatccgg gtttcagacg
    28741 gcatttgttt ttggctgtgt ctgaacaatc ggtacgattt cggttgtttg caaattgaaa
    28801 ggcggttatt cctatgttca ggcggcaaac cgaaatattt tccccaaccg atagggcgat
    28861 atcaaagcct attcgaatac cgcctttacg gggaaggggc tttccgagct tgaccatggt
    28921 caggctcggc aaatcagccc gcatcctgtc gatgcgtccg tccaaatcgg tttggaacgc
    28981 cgcaatgtga tatccgccta tcaaggtttt gcgtgcgtgt gtcggcgagg gctttgccaa
    29041 aaccgaaatc aaaatcaagt tgtcaggcat ccttccgttt ctattaaaat agcgcattcc
    29101 atttttcaga cggcatcctt atgtttcccg accaatccgc ccccaatctg ctgcaaggct
    29161 tgaaccccga acaactctcc gccgtaacct ggccgccgca atccgccctc gttttggcag
    29221 gcgcaggcag cggcaaaacg cgcgtgctga ccacgcgtat cgcatggctt ttgcaaagcg
    29281 gacaagccag cgtacacagc attatggcgg taacgtttac caacaaggcc gccaaagaga
    29341 tgcaaacccg attcggcgca atgattccca tcaacgtccg cgctatgtgg ctcggcacgt
    29401 tccacggttt gtgccaccgc tttttgcgcc tgcaccaccg cgacgcaggc ctgccgtctt
    29461 cctttcaaat cctcgacagc ggcgaccagc tttccctcat caaacgcctg ctcaaaagcc
    29521 tcaacatcgc cgaagaaatc atcgcgccgc gttcgctgca aggctttatc aacgcgcaaa
    29581 aagaatccgg tttgcgcgct tccgtcttgg gcgcgcccga tccgcacaca agccgcatga
    29641 ttgggtgcta cgccgaatac gacaaaatct gccaacgcga aggcgtggtc gatttcgccg
    29701 aactcatgct ccgcagctac gaaatgctgc aaagtaacga aatcctgcgc cggcactacc
    29761 aaaaccgctt caaccacatt ctggtcgacg agtttcaaga caccaacaaa ctgcaatacg
    29821 cctggctcaa actcatggcg ggcggtaacg cagcggtatt tgccgtcggc gacgacgacc
    29881 aaagcattta ccgcttccgc ggcgcaaacg tcggcaacat gaccgcactg atggaagaat
    29941 tccacatcga cgcgcccgtc aaactcgaac aaaactaccg ctccgtcggc aacatcctcg
    30001 ccgccgccaa cgccgttatc gaaaacaacg acgaacgtct cggcaaaaac ctgcgcaccg
    30061 acgccgaagc aggcgacaaa atccgctact actccgcctt taccgacctc gaagaagccc
    30121 ggttcatcgt ggatgaaacc aaagccctcg aacgcgaagg ctgggatttg gacgaaatcg
    30181 ccgtcctcta ccgcagcaac gcccaatcgc gcgtcatcga acaaagcctg ttccgcagcg
    30241 gcatccccta caaaatctac ggcggcctgc gcttttacga acgccaagaa atcaaacacg
    30301 ccctcgccta cctgcgcctc gccgtcaatc ccgacgacga caacgccctc ttgcgtgtca
    30361 tcaacttccc accgcgcggc atcggcgcgc gcaccgtcga aaaccttcag acggcctcaa
    30421 acgaacaagg catcaccctc tggcaggcag cctgcaacgc cggcgcgaaa gccgccaaag
    30481 tcgccgcctt cgtccgcctg attgaagccc tgcgcaacca agtcggacaa atgcacctgt
    30541 ccgaaatcat cgtcggcatc ctcaaagaca gcggtctgac cgagcactac cgaacccaaa
    30601 aaggcgacaa ccaagaccgc ctcgacaacc ttgacgaact cgtcaacgcc gccatcgagt
    30661 tcaaacccga agacagcaac tttgaaaccc tgcccgaaaa catttcagac gaccccgcct
    30721 tccccatcct cgccttcttg agcaacgccg ccctcgaatc cggcgaaaac caagcagggg
    30781 caggcgaaaa agccgtccaa ctgatgaccg tccacgccgc caaaggcttg gaattcaacg
    30841 ccgtcttcct caccggtatg gaagaggggc gtttccccag cgaaatgagc cttgccgagc
    30901 gcggcggcct cgaagaagaa cgccgcctca tgtacgtcgc catcacccgc gcccgcaaac
    30961 gcctctacat caccatggca cagcaacgca tgctgcacgg acaaacccaa ttcggcatcg
    31021 catcccgctt cgtcgaagaa atcccgccag aagtattgca ctacctgtcc gtcaaaaaac
    31081 ccgccttcga cagctacggc aacacgcgcc aaaccaccgt acaacgggac aaaatcatcg
    31141 atgacttcaa acagccgcaa acctacgcag gcttccgcat cggacaaaac gtccgtcacg
    31201 ccaaattcgg caccggcgtg attatcgatg ccgcagacaa aggcgaatcc gcccgactga
    31261 ccatcaactt cggcaaacag ggcgtgaaag agctggatac caagtttgcg aaattggaag
    31321 cgatgtaaat ttgaaatgca ggccggatat tcgtatccga caaaaaaaca tttgacgcgt
    31381 ttatcgtttc cgaaaaaccg ctgttggaaa tgtcggattc gagaatccga cctacggcaa
    31441 aaaaccgtag caaggacaag gcaaatagtc gtctgaaaac gggaagggca attttgccgc
    31501 agccgccgcc gtcattcccg cgaacctaca ttccgtcatt cccacgaaag tgggaatccg
    31561 gaacgcaaaa tctaaagaaa ccgttttatc cgataagttt ccgcaccgac aggtctggat
    31621 tcccgcctgc gcgggaatga cggcggcgga ttcggcggag ttggtggatt tggcgggctg
    31681 aagcccgccc ttgtatatcg gaattaccgt atcatagcga caaaccgccc ggccgccacc
    31741 cgcgcccgcc caaggcagac aaccgttgcg cggctcaggg agcggcaggg caacccatcg
    31801 gcacaaccgg
//
LOCUS       DAWTQS010000017        31747 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.017, whole genome shotgun sequence.
ACCESSION   DAWTQS010000017 DAWTQS010000000
VERSION     DAWTQS010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 31747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31747
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.017"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            58..444
                     /locus_tag="WNA25_000796"
                     /pseudo
     CDS             58..444
                     /locus_tag="WNA25_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(827..1717)
                     /gene="sucD"
                     /locus_tag="WNA25_000797"
     CDS             complement(827..1717)
                     /gene="sucD"
                     /locus_tag="WNA25_000797"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFA9823596.1"
                     /translation="MSVLINKDTKVLVQGFTGKNGTFHSEQALAYGTKVVGGVTPGKG
                     GQTHLDLPVFNTMKEAVKETGADASVIYVPAPFVLDSIVEAVDSGVGLVVVITEGVPT
                     LDMLKAKRYLETNGNGTRLVGPNCPGVITPGECKIGIMPGHIHTPGRIGIISRSGTLT
                     YEAVAQTTKLGLGQSTCIGIGGDPIPGMNQIDALKLFQEDPDTDAIIMIGEIGGTAEE
                     EAAEYIQSNVTKPVVGYIAGVTAPKGKRMGHAGAIISGGKGTAEEKFAAFEKAGIAYT
                     RSPAELGTTMLEVLKAKGLA"
     gene            complement(1728..2894)
                     /gene="sucC"
                     /locus_tag="WNA25_000798"
     CDS             complement(1728..2894)
                     /gene="sucC"
                     /locus_tag="WNA25_000798"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFA9823597.1"
                     /translation="MNLHEYQAKELLASYGLPVQGGILAHNGEEAAAAYDKLGGKFAV
                     VKAQVHAGGRGKAGGVKVVKSREKAKEVAESLIGTNLVTYQTDANGQPVNSVLVCEDM
                     YPVQTELYLGAVVDRSTRRVTFMASTEGGVEIEKVAAETPEKIFKVTVDPLVGLQPCQ
                     AREVAFQLGLKDKQINEFAKLMTGAYKAFVENDFALFEVNPLAVRENGALACVDGKIG
                     IDSNALYRLPKIAELRDKSQENERELKASEFDLNYVALEGNIGCMVNGAGLAMATMDI
                     IKLKGGQPANFLDVGGGATKDRVVEAFKLILEDKSVKGVLINIFGGIVRCDMIAEAIV
                     AAVKEINVNVPVVVRLEGNNAELGAKILNESGLKLTSADGLNDAAEKIVAAVNA"
     gene            complement(2966..3253)
                     /locus_tag="WNA25_000799"
     CDS             complement(2966..3253)
                     /locus_tag="WNA25_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823598.1"
                     /translation="MNKEIVGIFFIPMGIISMCMAALWQMYVMMTETYTLNRFKDKEL
                     VWRVALLFISFSLAVYLLCPNSRKKGIVFFILGGGGAVMYLLARMWLPFSK"
     gene            complement(3373..4806)
                     /gene="lpdA"
                     /locus_tag="WNA25_000800"
     CDS             complement(3373..4806)
                     /gene="lpdA"
                     /locus_tag="WNA25_000800"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFA9823599.1"
                     /translation="MSQYDVVVIGAGPGGYVAAIRAAQLGFKTACVDAGVNKAGNAPA
                     LGGTCLNVGCIPSKALLQSSEHFHAAQHDFAEHGITVGDVKFDVAKMIERKDAIVTKL
                     TGGVKFLFQKNKVTSLFGTASFAGKNGDAYQIEVDNKGEKTVIEAKHVIVATGSVPRP
                     LPQVAIDNVNVLDNEGALNLTEVPAKLGVIGSGVIGLEMGSVWNRVGAEVTILEAAPT
                     FLAAADQQIAKEAFKYFTKEQGLSIELGVKIGDIKSEGKGVSVAYETAAGEAKTEVFD
                     KLIVAIGRIPNTKGLNAEAVGLEKDERGFIKVDGECRTNLPNVWAIGDVVRGPMLAHK
                     ASDEGVAVAERIAGQKPHIDFNNVPFVIYTDPEIAWVGKTEEQLKAEGVEYKKGTSGF
                     GANGRALAMGKAKGTVKVLADAKTDRILGVHMIGPVVSELVTEGVTALEFFASSEDIA
                     RIIHAHPTLSEVVHEAALAADKRALHG"
     gene            complement(5067..6248)
                     /gene="odhB"
                     /locus_tag="WNA25_000801"
     CDS             complement(5067..6248)
                     /gene="odhB"
                     /locus_tag="WNA25_000801"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFA9823600.1"
                     /translation="MIIDVKVPMLSESVSEGTLLEWKKKVGEAVARDEILIDIETDKV
                     VLEVPSPQAGVLVEIVAQDGETVVADQVLARIDTAATVAAEAPAAAPAEAAPAAVPAA
                     AQNNAAMPAAAKLAAETGVDVNVLQGSGRDGRVLKEDVQNAAAKPAAAVAPAVALPAG
                     ARPEERVPMSRLRARVAERLLASQQENAILTTFNEVNMKPIMDLRAKYKEKFEKEHGV
                     KLGFMSFFVKAAVAALKKYPVVNASVDGKDIVYHGYFDIGIAIGSPRGLVVPILRDAD
                     QMSIADIEQAIVDYAKKAKDGKIAIEDLTGGTFSITNGGTFGSMMSTPIINPPQSAIL
                     GMHATKERAVVENGQVVVRPMMYLALSYDHRIIDGREAVLTLVAIKDALEDPVRLLLD
                     L"
     gene            complement(6348..9176)
                     /locus_tag="WNA25_000802"
     CDS             complement(6348..9176)
                     /locus_tag="WNA25_000802"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFA9823601.1"
                     /translation="MMDEKLNFSYLFGSNAPYIEELYEAFLENPDAVDEKWKQYFTDL
                     SKQPGTVAVDVAHTPIRESFVTLAKKKIASAVAGGADEAMLKKQVSVLRLISAYRIQG
                     VGAAQLDPLKRIPPRDIEALDPKFHGLSDADMALRFNMGEGDFANRGKLPLSQIISNL
                     KQTYCGHIALEYIYIPNTEERRWVRNYFESVLSTPHYNADQKRRILKEMTAAETLERY
                     LHTKYVGQKRFGVEGGESAIAGLNYLIQNAGKDGVEEVIIGMAHRGRLNVLVNILGKK
                     PGDLFAEFEGRAEIKLPSGDVKYHMGFSSDIATPHGPMHVSLAFNPSHLEIVNPVVEG
                     SARAKQKRLGENGRDKVLPVLIHGDSAFIGLGVNQATFNLSKTRGYTTGGTVHIVINN
                     QIGFTTSDIRDTRSTVHCTDIAKMVSAPVIHVNGDDPERVCFAIQAALDYRKKFHKDI
                     VIDVVCYRKWGHNEGDDPTLTQPMMYKKVSQHPGARALYTEQLIAEGVVTQVEADGYI
                     QAYRDALDKGEHVEQTTLSNFQRTQIDWSKYQGKDWREKIETGLPAADIERLTEKFTA
                     VPEGFALHPTAKRVIEARKAMASGKQAIDWGMAETLAYASLLTKGHGVRISGEDSGRG
                     TFSHRHAVLHDQKREKWDDGTYVPLRNMGEGLGEFLVIDSILNEEAVMAFEYGFACSA
                     PDKLTIWEAQFGDFANGAQVTIDQFLSSGETKWGRLCGLTTILPHGYDGQGPEHSSAR
                     VERWLQLCSENNMQVIMPSEASQMFHLLQRQVLGSYRKPLVIFMSKRLLRFKGAMSPL
                     ENFTEGSTFRPVIGDTAERASNDSVKRVVLCAGQVYYDLEAGRAERKLEDDVAIVRVE
                     QLYPFPYDEVKAELAKYPNAKSVVWAQEEPKNQGAFYQIRHRIEDVISEEQKLSYVGR
                     PSSASPAVGYSSKHIAQLKQLVEDALAL"
     gene            complement(9381..10664)
                     /gene="gltA"
                     /locus_tag="WNA25_000803"
     CDS             complement(9381..10664)
                     /gene="gltA"
                     /locus_tag="WNA25_000803"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HFA9823602.1"
                     /translation="MSKSIKLNVPGRAGLELPVLEASIGHDVVDIRGLTKNTGLFAFD
                     PGFVSTASCESKITYIDGDQGLLYYRGYPIEQLAEKSDYLEVCYLLIYGELPTPEQKA
                     EFDNTVRRHTMVHEQLTWFFRGFRRDAHPMAMMVGVVGALSAFYQDSLDITNPEHRKI
                     AIYRLISKIPTIAAMCYRYSNGLPFNYPKNNLSYSENFLHMMFATPCEDYKPNPVLAR
                     ALDRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGASHGGANEAVLKML
                     DEIGDVSNVAAYMEGVKQRKYRLMGFGHRVYRNMDPRASIMRETCYEVLKELGLEDSP
                     KFKLAMELEQIALKDPFFIERKLYPNVDFYSGIVLSALGIPTEMFTVIFALSRSVGWI
                     SHWHEMISDPSLKIGRPRQLYTGSERRDYVPAGER"
     gene            complement(10776..11024)
                     /locus_tag="WNA25_000804"
     CDS             complement(10776..11024)
                     /locus_tag="WNA25_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HFA9823603.1"
                     /translation="MMVFDDIAKRKIRFQTRRGLLELDLIFGRFMEKEFEHLSDKELS
                     EFSEILEFQDQELLALIHGHSETDKGHLIPMLEKIRRA"
     gene            complement(11028..11735)
                     /locus_tag="WNA25_000805"
     CDS             complement(11028..11735)
                     /locus_tag="WNA25_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HFA9823604.1"
                     /translation="MEKMSFEIYRYNPDVDAKPYMQRYELELEPTDVKLLDALVRLKA
                     QDDTLSFRRSCREGICGSDGMNINGKNGLACLTDLRSLKQPVKIRPLPGLPVIRDLIV
                     DMTQFFKQYHSVKPYVVNDNPIDADKERLQTQEERKELDGLYECILCACCSTACPSFW
                     WNPDKFVGPSGLLNAYRFIADSRDTITNERLDNLNDPYRLFRCHTIMNCVDVCPKHLN
                     PTRAIGKIKEIMLKRVV"
     gene            complement(11855..13618)
                     /gene="sdhA"
                     /locus_tag="WNA25_000806"
     CDS             complement(11855..13618)
                     /gene="sdhA"
                     /locus_tag="WNA25_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFA9823605.1"
                     /translation="MGFPVRKFDAVIVGGGGAGLRAALQLSKSGLNCAVLSKVFPTRS
                     HTVAAQGGISASLGNVQEDRWDWHMYDTVKGSDWLGDQDAIEFMCRAAPEAVIELEHM
                     GMPFDRVESGKIYQRPFGGHTAEHGKRAVERACAVADRTGHAMLHTLYQQNVRANTQF
                     FVEWTAQDLIRDENGDVVGVTAMEMETGEVYIFHAKAVMFATGGGGRIYASSTNAYMN
                     TGDGLGICARAGIPLEDMEFWQFHPTGVAGAGVLITEGVRGEGGILLNADGERFMERY
                     APTVKDLASRDVVSRAMAMEIYEGRGCGKNKDHVLLKIDHIGAEKIMEKLPGIREISI
                     QFAGIDPIKDPIPVVPTTHYMMGGIPTNYHGEVVVPQGDEYEVPVKGLYAAGECACAS
                     VHGANRLGTNSLLDLVVFGKAAGDSMIKFIKEQSDWKPLPANAGELTRQRIERLDSQT
                     DGENVDALRRELQRSVQLHAGVFRTDEILSKGVQEIMAIAERVKRTEIKDKSKVWNTA
                     RIEALELDNLIEVAKATLVSAEARKESRGAHASDDHPERDDENWMKHTLYHSDTNTLS
                     YKPVHTKPLSVEYIKPAKRVY"
     gene            complement(13621..13962)
                     /gene="sdhD"
                     /locus_tag="WNA25_000807"
     CDS             complement(13621..13962)
                     /gene="sdhD"
                     /locus_tag="WNA25_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HFA9823606.1"
                     /translation="MVERKLTGAHYGLRDWVMQRATAVIMLIYTVALLVVLFALPKEY
                     PAWQAFFSQAWVKVFTQVSFIAVFLHAWVGIRDLWMDYIKPFGVRLFLQVATIVWLVG
                     CLVYSVKVIWG"
     gene            complement(13956..14333)
                     /gene="sdhC"
                     /locus_tag="WNA25_000808"
     CDS             complement(13956..14333)
                     /gene="sdhC"
                     /locus_tag="WNA25_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HFA9823607.1"
                     /translation="MSVKLRPVYLDLPNIRLPIPGIVSILHRISGVGLFIMLPFLLYF
                     LSGTLSQESAFETYRAIVSHPLVKLVLIGILWAYLHHSLAGIRFLFLDAHKGLELNTA
                     RNTAKAVFASALVLTVVLGALLW"
     gene            complement(14620..16023)
                     /locus_tag="WNA25_000809"
     CDS             complement(14620..16023)
                     /locus_tag="WNA25_000809"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFA9823608.1"
                     /translation="MKKIQADIVVIGGGTAGMGAFRNARLHSDNVYLIENNVFGTTCA
                     RVGCMPSKLLIAAAEARHHALHTDPFGVHLDKDSIVVNGEEVMRRVKSERDRFVGFVV
                     TDVEEWPADKRIMGSAKFIDEHTVQIDDHIQIAAKSFVIATGSRPVILPQWQSLGDRL
                     IINDDVFSWDTLPKRVAVFGPGVIGLELGQALHRLGVKVEIFGLGGIIGGISDPVVSD
                     EAKAVFGEELKLHLDAKTEVKLDADGNVEVHWEQDGEKGVFVAEYMLAAVGRRPNVDN
                     IGLENINIDKDARGVPVADPLTMQTSIPHIFIAGDASNQLPLLHEAADQGKIAGDNAG
                     RYPNIGSGLRRSTIGVVFTSPQIGFVGLKYAQVAAQYQADEFVIGEVSFKNQGRSRVM
                     LVNKGHMRLYAEKATGRFIGAEIVGPAAEHLAHLLAWAHQMKMTVPQMLDMPFYHPVI
                     EEGLRTALRDADAKLKA"
     gene            complement(16272..17009)
                     /locus_tag="WNA25_000810"
     CDS             complement(16272..17009)
                     /locus_tag="WNA25_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HFA9823609.1"
                     /translation="MALQDRTGQKVPSVVFRTRVGDTWKDVSTDDLFKGKKVVVFSLP
                     GAFTPTCSSSHLPRYNELFGAFKENGVDAICCVSVNDTFVMNAWAAEEESDNIYMIPD
                     GNGEFTEGMGMLVGKEDLGFGKRSWRYSMLVNDGVVEKMFIEPEEPGDPFKVSDADTM
                     LKFVAPDWKAQESVAIFTKPGCQFCAKVKQALQDKGLSYEEIVLGKDATVTSVRAITG
                     KMTAPQVFIGGKYIGGSEDLEAYLAKN"
     gene            complement(17288..19564)
                     /gene="metE"
                     /locus_tag="WNA25_000811"
     CDS             complement(17288..19564)
                     /gene="metE"
                     /locus_tag="WNA25_000811"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFA9823610.1"
                     /translation="MTTLHFSGFPRVGAFRELKFAQEKYWRKEISEQELLDVAKDLRE
                     KNWKHQAAANADYVAVGDFTFYDHILDLQVATGAIPARFGFDSQNLPLEQFFQLARGN
                     KDQFAIEMTKWFDTNYHYLVPEFHADTEFKANAKHYVQQLQEAQTLGLKAKPTVVGPL
                     TFLWVGKEKGSVEFDRLSLLPKLLPVYVEILTALVEAGAEWIQIDEPALAVDLPKEWV
                     EAYKDVYATLNKVSAKILLGTYFGSVAEHAALLKSLPVDGLHIDLVRAPEQLDAFAGY
                     DKVLSAGVIDGRNIWRANLNKVLETVGPLQAKLGERLWISSSCSLLHTPFDLSVEEKL
                     KANKPDLYSWLAFTLQKTQELRVLKAALNEGRDSVAEELAASQAAADSRANSSEIHRA
                     DVAKRLADLPVNADQRKSPFADRIKAQQAWLNLPLLPTTNIGSFPQTTEIRQARAAFK
                     KGELSASDYEAAMKKEIALVVEEQEKLDLDVLVHGEAERNDMVEYFGELLSGFAFTQY
                     GWVQSYGSRCVKPPIIFGDVSRPEAMTVAWSTYAQNLTKRPMKGMLTGPVTILQWSFV
                     RNDIPRATVCKQIALALNDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLNWAG
                     ESFRLSSAGCEDSTQIHTHMCYSEFNDILPAIAAMDADVITIETSRSDMELLAAFGEF
                     KYPNDIGPGVYDIHSPRVPTEAEVEHLLRKAIEVVPVERLWVNPDCGLKTRGWKETLE
                     QLQVMMNVTRKLRAELAK"
     gene            complement(19700..20578)
                     /gene="metF"
                     /locus_tag="WNA25_000812"
     CDS             complement(19700..20578)
                     /gene="metF"
                     /locus_tag="WNA25_000812"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFA9823611.1"
                     /translation="MNYAKEINALNNSLSDLKGNINVSFEFFPPKNEQMETMLWDSIH
                     RLQTLHPKFVSVTYGANSGERDRTHGIVKRIKQETGLEAAPHLTGIDASHDELRQIAK
                     DYWDSGIRRIVALRGDEPAGYEKKPFYAEDLVKLLRSVADFDISVAAYPEVHPEAKSA
                     QADLINLKRKIDAGANHVITQFFFDVERYLRFRDRCVMLGIDVEIVPGILPVTNFKQL
                     GKMAQVTNVKIPKWLSQMYEGLDDDQGTRNLVAASIAIDMVKVLSREGVKDFHFYTLN
                     RSELTYAICHILGARP"
     gene            20775..21050
                     /locus_tag="WNA25_000813"
     CDS             20775..21050
                     /locus_tag="WNA25_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFA9823612.1"
                     /translation="MKPNIHPDNYRTVLFFDSSANEGWLIRSCAGTHGKTMVWTDGKE
                     YPLFSLDTSSSSHPVYTGKQRNVNTEGRASKFNQRFQSVMSSFRKDK"
     gene            21050..21175
                     /gene="ykgO"
                     /locus_tag="WNA25_000814"
     CDS             21050..21175
                     /gene="ykgO"
                     /locus_tag="WNA25_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFA9823613.1"
                     /translation="MQVLSSLKTAKQRHRDCQIVRRRGKVYVICKSNPRFKSRQR"
     gene            complement(21261..21479)
                     /locus_tag="WNA25_000815"
     CDS             complement(21261..21479)
                     /locus_tag="WNA25_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823614.1"
                     /translation="MIGRLFRIFFFFALAALIINRLFSRRQKRVLREVAEISAWVLLG
                     AAAATLFWYLFMLYFKHIPDSYRRKNAV"
     gene            21622..22761
                     /locus_tag="WNA25_000816"
     CDS             21622..22761
                     /locus_tag="WNA25_000816"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HFA9823615.1"
                     /translation="MSQNTSVGIVTPQKIPFEMPLVLENGKTLPRFDLMIETYGELNA
                     EKNNAVLICHALSGNHHVAGRHSAEDKYTGWWDNMVGPGKPIDTERFFVVGLNNLGGC
                     DGSSGPLSINPETGREYGADFPMVTVKDWVKSQAALADYLGIEQWAAVVGGSLGGMQA
                     LQWAISYPERVRHALVIASAPKLSTQNIAFNDVARQAILTDPDFNEGHYRSHNTVPAR
                     GLRIARMMGHITYLAEDGLGKKFGRDLRSNGYQYGYSVEFEVESYLRYQGDKFVGRFD
                     ANTYLLMTKALDYFDPAADFGNSLTRAVQDVQAKFFVASFSTDWRFAPERSHELVKAL
                     IAAQKSVQYIEVKSAHGHDAFLMEDEAYMRAVTAYMNNVDKDCRL"
     gene            22758..23339
                     /gene="metW"
                     /locus_tag="WNA25_000817"
     CDS             22758..23339
                     /gene="metW"
                     /locus_tag="WNA25_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HFA9823616.1"
                     /translation="MNLRDDLQLIYDRIPEGSRVLDLGCGDGELLAALAEHKKCSGYG
                     IEIDTDNVIAAMSRGVNVIQADLEEGLTAFNDQSFDVIVLSQTIQAMQNTEKILRCLM
                     RLAKQAIVSFPNFGYWRNRVQIALGGHMPVSERMPYHWYDTPNIHWCTLKDFDLLCAK
                     NNIRVLERAVMTGNRQVKHFPNLLGSLAFYRVG"
     gene            23456..23647
                     /locus_tag="WNA25_000818"
     CDS             23456..23647
                     /locus_tag="WNA25_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823617.1"
                     /translation="MKNKTSSLPLWLAAIMLAARSPSKEDKTKENGASAASSSASQSD
                     LQPAASAPDNVKQAESAPL"
     gene            23690..24253
                     /locus_tag="WNA25_000819"
                     /pseudo
     CDS             23690..24253
                     /locus_tag="WNA25_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675049.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="d-alanine-poly(phosphoribitol) ligase"
     gene            24251..24358
                     /locus_tag="WNA25_000820"
                     /pseudo
     CDS             24251..24358
                     /locus_tag="WNA25_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(24434..24994)
                     /gene="efp"
                     /locus_tag="WNA25_000821"
     CDS             complement(24434..24994)
                     /gene="efp"
                     /locus_tag="WNA25_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFA9823618.1"
                     /translation="MKTAQELRAGNVFMVGNDPMVVQKTEYIKGGRSSAKVSMKLKNL
                     LTGAASETIYKADDKFDVVILSRKNCTYSYFADPMYVFMDEEFNQYEIEADNIGDALK
                     FIVDGMEDQCEVTFYEGNPISVELPTIIVREVDYTEPAVKGDTSGKVMKTARLVGGTE
                     IQVMSYIENGDKVEIDTRTGEFRKRA"
     gene            complement(25037..26188)
                     /gene="earP"
                     /locus_tag="WNA25_000822"
     CDS             complement(25037..26188)
                     /gene="earP"
                     /locus_tag="WNA25_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HFA9823619.1"
                     /translation="MNTYAFPVCWIFCKVIDNFGDIGVSWRLARVLHRELGWQVHLWT
                     DDVSALRALCPDLPDVPFVHQDIHVRTWHSDAADIDTAPVPDAVIETFACDLPENVLN
                     IIRRHKPLWLNWEYLSAEESNERLHLMPSPQEGVQKYFWFMGFSEKSGGLIRERDYRE
                     AVRFDTEALRRRLVLPEKNAPEWLLFGYRGDVWAKWLDMWQQAGSLMTLLLAGAQIID
                     SLKQSGVIPQNALQNDGGVFQTASVRLVKIPFVPQQDFDKLLHLADCAVIRGEDSFVR
                     TQLAGKPFFWHIYPQDENVHLDKLHAFWDKAYGFYTPETASVHRLLSDDLNGGEALSA
                     TQRLECWQTLQQHQNGWRQGAEDWSRYLFGQPSASEKLAAFVSKHQKIR"
     gene            complement(26207..26485)
                     /locus_tag="WNA25_000823"
     CDS             complement(26207..26485)
                     /locus_tag="WNA25_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823620.1"
                     /translation="MSRNLLVRWLAVCLIPLATLAVFAANPPEDKPQHLINGIILACE
                     ATFLFKFVLFETIKHHLKQGFDLKRQTMFLFIPIVLLVVYLFHYFGAF"
     gene            26836..27777
                     /gene="miaA"
                     /locus_tag="WNA25_000824"
     CDS             26836..27777
                     /gene="miaA"
                     /locus_tag="WNA25_000824"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFA9823621.1"
                     /translation="MPTPKAFTLLGPTACGKTALALKIAETLPVEIISLDSALLYTGM
                     DIGTAKPSASERAFVPHHLIDIITPVQTYSAARFVEDCTRLTGEITARGKCPLIVGGT
                     MMYFRALTQGLNDLPEADACLRADLDEQKQMYGLDFLYRTLQKVDPETACRLKPNDSQ
                     RIGRALEVYYLTGRPMSAHLNGQPEHTLPFELYTAALIPENRARLHENIALRFHLMLE
                     QGFIGEVENLRRRYPGLTADSPAIRCVGYRQAWEHLDGATDRQTFIEKGIAATRQLAK
                     RQLTWLRKTPLDCVADPFSDGTSGTRLIEAAKRFFGE"
     gene            27781..28500
                     /locus_tag="WNA25_000825"
     CDS             27781..28500
                     /locus_tag="WNA25_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HFA9823622.1"
                     /translation="MLATPPLAPKTVAALHRLGIRTLEELRQNGSVKAFLLLKASGLT
                     LTKSTLWQLESLLNGTPPQEMSQAHKDCLLAELKNHPPVAAFPPQEEMGYFMREALRQ
                     AEQSAADGEIPVGAVIVSDGKIITSAHNTCIADCNVSRHAEINALAQAGSEMQNYRLD
                     GCDIYITLEPCAMCASALIQARIRRVIYGAAEPKTGAAGSIVNLFADKRLNTHTAIRG
                     GILQEECRAVLSRFFQNKRKG"
     gene            28505..28993
                     /locus_tag="WNA25_000826"
     CDS             28505..28993
                     /locus_tag="WNA25_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HFA9823623.1"
                     /translation="MTALLVILALALIAVGTAGIVYPALPGLALMFAGTWLLAYAGGY
                     QIYGAGILWTVGLISLGGILADYMAGMLGVKYTGAGKLAVRGALAGSIIGIFFSLPGL
                     ILGPFIGAAAGELIDRRNMLQAGKAGLGTLLGLVVGTAFKIGCAVSILLILLVKYIAY
                     LF"
     gene            29059..29811
                     /gene="rlmB"
                     /locus_tag="WNA25_000827"
     CDS             29059..29811
                     /gene="rlmB"
                     /locus_tag="WNA25_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFA9823624.1"
                     /translation="MANQRPIYGFHAVNARLWQNPKSITELYIQEGKSDARTREVLEK
                     AANENIRVYFADADRLNAISKGARHQGVVGFIDASKNHVHLEDVLENLSEPPLLLILD
                     GITDPHNLGACLRTADAMGVHAVIAPKDKSAGLNATVSKVACGAAETVPYITVTNLAR
                     TLRELKEYGIWIIGTDIGGNADLYHCNLPDSAAWVMGNEGDGMRRLTREHCDMLVSIP
                     MFGTVESMNVSVSAGMVLSETRRQRVLKNKKA"
     gene            complement(29873..31247)
                     /locus_tag="WNA25_000828"
                     /pseudo
     CDS             complement(29873..31247)
                     /locus_tag="WNA25_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223860.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease"
     gene            31627..>31747
                     /locus_tag="WNA25_000829"
     CDS             31627..>31747
                     /locus_tag="WNA25_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HFA9823625.1"
                     /translation="MLQGSLVALITPMNQDGSIHYDQLRQLIDWHIENGTDGIV"
BASE COUNT         7799 a         8385 c         7944 g         7619 t
ORIGIN      
        1 cactatatat agaaggtata tatcctagta ggcgtaatgg cagctattac aaaatgtctg
       61 catcaggttc acgaaacgca agtcgaaagc gtcaaacaac tgattgcccg ttttgaccgg
      121 ctgattgacg aatcggacaa acaaatcgac aaccacaccc gcacgcattt tgacggcaaa
      181 gcccaagtgg cggagcaaat caaaggcatc ggttcgataa cgacggctac gctgatggcg
      241 atgctgcccg aattggggcg gctgtcgcac aaacggatag cgggtttggt cggcattgcc
      301 ccgcacccga gggagagcgg ggaaaccaaa ttcaaaagcc gctgcttcgg cggaaggtct
      361 gcggtgcgta aggcgctgta tatggctgcc gcggcagcga cacgttttga accgcttatt
      421 cgggatttcc accaacgcct gctgtatagc ggattaacaa aaaccggtac ggcgttgccc
      481 cgccccggct caaagggaac gattccctaa ggtgatgaag caccgggcgg atcggttccg
      541 taccatttgt accgtctgcg gcccgccgcc ttgtcctgat ttttgttaat ccgctatggt
      601 ttaaagcttt gtttcttaag tccgcagagt atgccatggt tagaccttca acgttgagtg
      661 ttgtactatt ttgtttttaa tccactatat tctgactgcc tcaaagggca aaaataatgt
      721 gagtatgttg aggctgcctc tttttgaaat gacaaacaaa atgccgtctg aaacccgatt
      781 tccggtttca gacggcattt gatgttcaat cagttgtcaa acctgattat gccaaacctt
      841 ttgctttcaa cacttccagc atcgtcgtgc ccaactcggc agggctgcgg gtgtaggcga
      901 taccggcttt ttcaaacgcg gcgaattttt cttcggcagt acctttaccg ccggagataa
      961 tcgcaccggc gtggcccatg cgtttgcctt taggagcggt aacaccggcg atgtaaccga
     1021 caacaggttt ggttacgttg gattggatgt attcggctgc ttcttcttcc gcagtaccac
     1081 cgatttcacc aatcatgatg atggcatcgg tatctgggtc ttcttggaag agtttcagtg
     1141 cgtcgatttg gttcatacca ggaatcgggt cgccgccgat accgatacag gttgattgac
     1201 ccaagcccag tttggtggtt tgtgccacgg cttcgtaagt caatgtaccg gaacgggaaa
     1261 taataccgat gcggcccgga gtgtggatgt ggcccggcat aatgccgatt ttgcactcgc
     1321 ccggagtaat cacgcccggg cagttagggc cgaccaaacg ggtaccgttg ccgttggttt
     1381 ccaagtaacg tttggctttg agcatgtcca gagttggaac gccttcggta atcacaacga
     1441 ccaagcctac gcctgaatca acggcttcaa cgatagaatc caacacaaac ggagcgggaa
     1501 cgtaaatcac ggatgcgtca gcgccggttt ctttaacggc ttctttcatg gtgttgaata
     1561 caggcaggtc taggtgggtt tgaccgcctt tgcctggggt aacgccgccg acaactttag
     1621 tgccgtaagc cagagcttgt tcggagtgga aagtaccgtt tttaccggtg aaaccttgaa
     1681 ccaatacttt ggtgtcttta ttaatcaata cgctcattct tttctcctta ggcgtttacg
     1741 gctgcaacaa ttttttcggc tgcgtcattc agaccgtctg cagaagtcag tttcagacct
     1801 gattcgttca ggattttcgc gccgagttcg gcgttgttgc cttccaaacg aacaacgaca
     1861 ggaacgttga cgttgatttc tttaacggct gccacgatgg cttccgcaat catgtcgcaa
     1921 cgtacgatac cgccgaagat gttgatcaat acgcctttaa cggatttgtc ttccaaaatc
     1981 agtttgaacg cttcaaccac gcggtctttg gttgcgccgc cgccaacgtc caagaagttg
     2041 gcaggttggc cgcctttgag tttgatgatg tccatggtgg ccatcgccaa accggcaccg
     2101 ttaaccatac agccgatatt accttccagg gcaacatagt tcaggtcgaa ttcagaagct
     2161 ttcagttcac gttcgttttc ttgagatttg tcgcgcaatt cagcgatttt tggcaggcgg
     2221 tagagcgcgt tgctgtcgat gccgattttg ccgtccacgc aagccagtgc gccgttttcg
     2281 cgaactgcca gcgggttgac ttcaaacagg gcgaagtcgt tttcgacaaa cgctttgtac
     2341 gcgccggtca tcagtttggc gaactcgttg atttgtttgt ctttcaagcc cagttggaag
     2401 gcaacttcgc gagcttggca aggttgcagg ccgaccagcg gatcaacggt tactttgaag
     2461 attttttctg gagtttcagc agcaactttt tcgatttcca cgccgccttc ggtagaagcc
     2521 atgaatgtaa cgcggcgggt agaacggtca accactgcgc ccaagtacag ctcggtttga
     2581 acaggataca tgtcttcgca aaccaaaacg ctgttgacag gttggccgtt ggcatcggtt
     2641 tggtaagtta ccaagttggt gccaatcagg ctttcagcca cttctttggc tttttcgcgg
     2701 cttttaacga cttttacgcc gcccgcttta ccgcggccgc cggcgtgtac ctgtgctttg
     2761 acaacagcga atttgccgcc caatttgtcg taagctgcag cggcttcttc gccgttgtgc
     2821 gccaaaatac cgccttgtac gggcaaaccg tagctagcca gcagttcttt agcctgatac
     2881 tcgtgtaaat tcatggattt ctccttaagg tgagtgggtt ttaggaatcg ccctgcggcc
     2941 aatcccatat tttcagacgg tcctactatt tactgaaggg caaccacatc cgcgccagca
     3001 gatacatgac tgcaccgcct cccccgagaa taaaaaagac gatgcctttt ttacgcgaat
     3061 tcggacagag cagataaacg gcaaggctga aactgataaa caacaatgcc acgcgccaaa
     3121 ccaattcttt atctttgaaa cggttgagcg tataagtttc ggtcatcatc acatacatct
     3181 gccacaatgc ggccatacac atgctgatga tgcccatcgg tataaagaaa ataccgacta
     3241 tttctttgtt cataatgaat gtaaatgctg tttgtctgac ggaattataa atcagaaaac
     3301 gaagctctcg gaacattcaa tttgccggag gccttaaggc cgtctgaaaa tttcagacgg
     3361 ccttaatgtc tatcaaccgt gcaaagcgcg tttgtcggcc gccaatgcag cttcgtgaac
     3421 cacttcggac aaggttgggt gggcatggat aatacgggcg atatcttcgc tgctggcgaa
     3481 gaattcgagc gcagtcacgc cttcggtaac caattcgctg acaaccgggc cgatcatgtg
     3541 tacgcccaag atacggtcgg ttttggcatc tgccaacact ttaaccgtac ctttggcttt
     3601 gcccattgcc aatgcacggc cgtttgcacc gaaacctgaa gtaccttttt tgtactccac
     3661 gccttcggct ttgagttgct cttcggtttt acccacccaa gcgatttcag ggtcggtgta
     3721 aatcacaaac ggtacgttgt tgaagtcgat atgcggtttt tgaccggcaa tgcgctcggc
     3781 aacggcaacg ccttcgtcgc tggctttgtg tgccaacatc gggccgcgaa ccacgtcgcc
     3841 gattgcccat acgttaggca ggttggtacg gcattcgcca tccactttga taaagccgcg
     3901 ctcgtctttt tccaagccta cggcttcagc gttcaggcct ttggtgtttg gaatacggcc
     3961 gatggcaacg atcagtttgt cgaatacttc ggttttggct tcgccggcag cagtttcgta
     4021 agcaacggaa acacctttgc cttcagactt gatgtcgccg attttcacac ccaattcgat
     4081 gctcagacct tgctctttgg tgaagtattt gaaggcttct ttggcgattt gttggtcggc
     4141 ggcagccagg aaggtcggcg cggcttcaag aatggtaact tccgcaccca cgcggttcca
     4201 tacggaaccc atttccaaac caatcacacc ggaaccgatt acgccgagtt tggcaggtac
     4261 ttcggtcagg ttcaatgcgc cttcgttgtc caatacgttc acgttgtcga tagcgacttg
     4321 cggcaacgga cgcggtacgg aaccggttgc tacgatgacg tgtttggctt cgataacggt
     4381 tttctcgcct ttgttatcga cttcaatttg gtaagcatcg ccatttttac cggcaaagga
     4441 agcagtaccg aacaggctgg ttactttgtt tttttggaac aggaatttca cgccgccggt
     4501 cagtttggtc acgatggcat ctttgcgctc aatcattttg gccacgtcaa attttacgtc
     4561 gccgacagtg ataccgtgtt cggcaaaatc gtgttgcgca gcgtggaaat gttcgctgga
     4621 ttgcaacagg gctttagaag ggatacagcc tacgttcaag caagtaccgc ccaatgcagg
     4681 ggcattgcct gctttgttaa cgcctgcatc gacacaggca gttttgaaac ccagttgtgc
     4741 ggcacggatg gcggcaacgt atccgcccgg acctgcacca atcactacta catcatattg
     4801 agacatcgtg tcatcctttg cttttagcag tatctcaaaa taaaaacgat acttttcgtt
     4861 tcttttttta ttttgaatga ctgcgttttg atggttttaa gaaaaagccg tctgaaagtc
     4921 catatctgcc gattcattcg aatcaaatgt ttcagacggc ctcaatcgtt cgttgagcct
     4981 attgtagcac gatactacat tacgctacat ctaaataaaa attcagacgg ccttttcatt
     5041 aacaaataaa aagccgtctg aaacgattac agatccaaca acaggcggac cgggtcttcc
     5101 aacgcgtctt taatggctac caaggtcaat acagcttcgc ggccgtcaat gatacggtgg
     5161 tcgtaagaca gagccagata catcattgga cggacaacaa cttggccgtt ttcaaccaca
     5221 gcgcgctctt tagtggcatg catacccaaa atcgcagatt gaggcgggtt gatgatcggg
     5281 gtggacatca tagaaccgaa agtaccgccg ttggtaatac tgaatgtacc gccggtcagg
     5341 tcttcgatag cgattttgcc gtctttggct tttttcgcgt aatcaacaat tgcttgttcg
     5401 atgtcggcaa tgctcatttg gtcggcatcg cgcaggattg gtacaaccaa accgcgtggg
     5461 ctgccaattg cgataccgat gtcgaagtag ccgtggtaca caatgtcttt gccgtcaaca
     5521 gaagcattca caaccgggta ttttttcagg gcggcaacag cggctttaac gaagaaggac
     5581 ataaagccca gttttacgcc gtgttctttc tcgaattttt ctttgtactt cgcacgcaag
     5641 tccatgatcg gtttcatgtt gacttcgttg aatgtagtca gaatggcgtt ttcttgttga
     5701 gaagccaaga ggcgttctgc aacacgggca cgcaggcggc tcattggtac gcgttcttca
     5761 ggacgtgcgc cggcaggaag tgcaacggca ggggccacgg ctgcggcagg tttggcagcg
     5821 gcattttgta cgtcttcttt caatacgcga ccgtcacggc cggaaccttg caatacgttc
     5881 acgtcaacac cggtctcggc agccagtttt gcagcggcag gcatggcggc gttgttttgt
     5941 gcagcagcag gaacggcagc cggggcagct tcggcaggag cggctgcagg tgcttcagca
     6001 gcgacagtag cagctgtatc gatacgggcc aaaacttggt cggcaacaac ggtttcaccg
     6061 tcttgcgcta cgatttcaac caatacgccg gcttgtggag aaggtacttc caaaaccact
     6121 ttgtccgttt cgatatcgat caggatttcg tcacgggcaa ctgcttcgcc aacttttttc
     6181 ttccattcca agagtgtgcc ttcagatacg ctttcagaca gcataggtac ttttacatca
     6241 ataatcattt tgtgtctcca atggcccttt cagacggcct gttgtgtgtt tttaattctg
     6301 catatgggca ttccgcctga gcagactcag acggaatgct acttggttta taacgccaaa
     6361 gcgtcttcaa ccaattgttt caattgagca atgtgtttgc ttgagtagcc cactgcaggc
     6421 gatgcgctgc ttggacggcc gacataagac agtttttgct cttcgctgat aacgtcttcg
     6481 atgcggtggc ggatttggta gaacgcgcct tggtttttcg gctcttcttg cgcccaaacc
     6541 acagattttg cgttcggata tttcgccagt tcggctttaa cctcgtcgta tgggaacgga
     6601 tacagctgct caacgcggac gatagcgaca tcatcttcca gtttacgttc ggctcgaccg
     6661 gcttccaagt catagtaaac ctgaccggca cacaatacca cgcgtttcac gctgtcgttg
     6721 cttgcgcgtt cggcggtatc accaataacc ggacggaaag tcgaaccttc ggtgaagttt
     6781 tccagcgggc tcattgcacc tttgaagcgc aacaggcgtt tggacatgaa aatcaccagc
     6841 ggtttgcggt atgaacccaa gacttggcgt tgcaagagat ggaacatttg cgacgcttca
     6901 gacggcatga tgacttgcat attgttctca gaacacagtt gcaaccaacg ttctacgcgt
     6961 gcagaagagt gttcagggcc ttgaccgtcg tagccgtgcg gcaggatggt ggtcagaccg
     7021 cacaagcgac cccacttggt ttcgcctgaa gacaggaatt gatcaatagt tacttgcgcg
     7081 ccgttggcga agtcaccgaa ttgagcttcc caaatggtca gcttgtcagg agcggagcag
     7141 gcaaagccgt actcgaacgc catcacggct tcttcgttca agatagagtc gataaccagg
     7201 aactcgccca agccttcgcc catgttgcga agaggaacat aagtaccgtc gtcccatttt
     7261 tcgcgttttt gatcgtgcag aacggcatga cggtgtgaga atgtaccgcg gccggagtcc
     7321 tcaccggaga tacgcacgcc atgacctttg gtcagcaggc ttgcgtatgc gagggtttcg
     7381 gccatacccc agtcgatggc ttgtttgccg gatgccatgg ctttacgcgc ttcgatcaca
     7441 cgttttgcag tcggatgcag ggcaaagcct tccggcacag cggtgaattt ctcagtaaga
     7501 cgctcaatgt cggcggcagg caaaccggtt tcgatttttt cgcgccaatc tttaccttgg
     7561 tatttgctcc agtcgatttg tgtgcgttgg aagttgctca acgttgtttg ctcaacatgt
     7621 tcgcctttgt ccaaagcatc acggtaggct tggatgtaac cgtcggcctc aacttgagtt
     7681 accacgcctt cggcaatcag ttgctcggtg tacaaagcac gcgcacccgg atgttgcgat
     7741 acttttttgt acatcatcgg ttgggtcaaa gtcggatcat cgccctcgtt gtgaccccat
     7801 ttacggtagc agacaacgtc gattacgatg tctttatgga attttttgcg gtaatccaaa
     7861 gcggcttgaa tggcaaagca aacgcgttcg ggatcatcgc cgttcacatg gataaccgga
     7921 gcggaaacca tttttgcgat atcggtacag tgtacggttg aacgggtatc gcggatatcg
     7981 gaagtggtaa agccgatttg gttgttgatg acgatatgga ccgtaccgcc tgtggtataa
     8041 ccgcgcgttt tagacaggtt gaatgttgct tggttgacac ccaaaccgat aaatgcggaa
     8101 tcgccgtgaa tcaataccgg caagacttta tcgcggccgt tttcgcccaa acgtttttgt
     8161 ttggcgcgtg cagaaccttc cactaccgga ttgacgattt ccaagtgtga cgggttgaac
     8221 gccaaagaaa cgtgcatcgg gccgtgaggg gtggcaatat cggagctgaa gcccatatgg
     8281 tatttcacgt cgccactagg cagtttgatt tcggcacggc cttcaaattc ggcaaacaaa
     8341 tcgccgggtt ttttgcccaa aatgttcacc aaaacattca gacggccacg gtgcgccata
     8401 ccgatgatga cttcttccac gccgtcctta ccggcgtttt gaatcaggta gttcaaaccg
     8461 gcaatcgcgc tttcgccgcc ttcgacaccg aaacgtttct gaccgacata tttggtatgc
     8521 agataacgtt ccaaagtctc ggcagcggtc atctctttca agatacggcg tttttgatcg
     8581 gcattgtaat gcggtgtgga caatacgctt tcaaaatagt tgcgtaccca acggcgctct
     8641 tcggtattgg gaatatagat atattccaat gcgatgtggc cgcagtaggt ttgtttgagg
     8701 ttgctgatga tttgggacaa gggcagtttg ccgcgattgg caaaatcacc ctcgcccata
     8761 ttgaatcgaa gcgccatatc ggcatctgac agaccgtgga atttcggatc gagggcttca
     8821 atatcgcgcg gagggatacg tttgagcgga tcaagttggg ccgcacccac gccttggata
     8881 cgataggcag aaatcagccg taaaacgctg acttgctttt tcagcattgc ctcatccgca
     8941 ccgcccgcaa cggcagatgc aattttcttt ttcgccaaag taacaaatga ttcgcgaatc
     9001 ggtgtgtgtg cgacatcgac agcaaccgtc cccggctgtt tgctcaaatc ggtgaaatac
     9061 tgcttccatt tttcatcaac cgcatcgggg ttttccaaaa aagcctcgta caattcctca
     9121 atgtagggtg cgttcgaacc gaacagataa gagaaattga gtttttcgtc catcatggcg
     9181 tgcgctcttt tctttaaaat ataaacaaag ggtagaagtc cgcgtcctga cggctgaact
     9241 gatgccgtct gaaaagacat tttattctac cctattttaa accggattgc ttgtcttgat
     9301 ttcatccgac tacaatcaga cggattcagg aaacaatccg gttctctgat attgcctgtt
     9361 tgacaatata ttaatgtatg ttacctctcg cctgccggca cataatcgcg gcgttccgaa
     9421 ccggtataaa gctggcgcgg gcggccgatt ttcagcgaag gatcgctaat catctcgtgc
     9481 cagtgcgaaa tccagcccac gctgcgcgac agggcgaaga tgacggtaaa catttcggtc
     9541 gggatgccca gcgcggacag gacgatgccg gaatagaaat cgacattggg atacagtttg
     9601 cgttcgataa agaacgggtc tttcagcgca atctgttcca attccatcgc cagtttgaat
     9661 ttcggactgt cttccaagcc caattccttc aaaacttcat agcaggtttc gcgcataatg
     9721 ctggcacgcg gatccatatt gcggtatacc cggtgtccga agcccatcag gcggtatttg
     9781 cgctgtttca cgccttccat gtatgcggca acattggaca catcgccgat ttcatccagc
     9841 attttcaaca cggcttcgtt cgcaccgccg tgcgaagcac cccacaggca ggcgataccg
     9901 gcagcaatac aggcaaacgg gttcgcaccc gaagagcctg ccagacggac ggttgaagtt
     9961 gaggcgtttt gctcgtggtc ggcatgcaaa ataaagatgc ggtcgagtgc gcgtgccaaa
    10021 acgggattag gtttgtagtc ttcacacggt gtggcaaaca tcatatgaag gaagttttcg
    10081 gaataagaaa gattattctt cggataattg aacggcagac cgtttgaata gcggtaacac
    10141 attgccgcaa tggtcggaat tttggaaata aggcggtaaa tcgcgatttt gcggtgttcg
    10201 ggattagtaa tgtccaagct gtcttggtag aacgcagaca gcgcgccgac cacgccgacc
    10261 atcatcgcca tcggatgcgc gtcgcggcgg aaaccccgga agaaccaagt cagctgttca
    10321 tgcaccatcg tgtggcggcg gactgtattg tcaaattctg ccttttgctc gggagtcggc
    10381 agttcgccgt aaatcaacag gtagcagact tccaaataat cggacttttc ggccagctgc
    10441 tcgatggggt atccgcgata ataaagcaag ccttgatcgc cgtcgatgta agtaatttta
    10501 gactcacagc ttgcggttga aacaaatccg gggtcgaagg caaacaaacc tgtatttttt
    10561 gtcagccccc gaatgtcaac cacatcatgc ccgatgctgg cttccaatac cggcagctcc
    10621 aaacctgccc gacccggtac gttgagtttg attgatttgg acatattatc gctcctttgc
    10681 aagttataac ccacgttact gcatccgcag cacgttcttt gctttcagac ggcctttgca
    10741 ttttgaaatc tgccctcaga caaatttcag atgcctcatg cccgtctgat tttttcaagc
    10801 atcgggataa ggtgcccttt gtccgtttcc gaatgcccgt gaatcaaggc aagcaattct
    10861 tgatcttgaa attcaaggat ttcggaaaac tcggacagct ctttatcgct caaatgctcg
    10921 aattcttttt ccataaacct gccgaagatt aaatctagtt ccaacaatcc ccggcgggtt
    10981 tgaaaacgga ttttccgttt ggcaatgtcg tcaaaaacca tcatttctta aacaacccgt
    11041 ttcaacataa tctctttaat cttaccgatg gcccgggtcg gattcaagtg tttgggacat
    11101 acgtccacgc agttcataat ggtgtggcag cggaacaaac ggtacggatc gttcaaatta
    11161 tccaaacgct cattagtgat ggtatctcgg ctgtccgcaa tgaagcggta ggcgttcagc
    11221 aagccggacg gaccgacgaa tttgtcggga ttccaccaga atgacggaca ggcggtcgaa
    11281 cagcaggcgc acaaaataca ttcgtacaaa ccatccaact ctttgcgctc ttcctgagtt
    11341 tgcagacgct ctttgtccgc atcaatcgga ttgtcgttaa caacataagg tttgacggaa
    11401 tggtattgtt tgaagaactg ggtcatatcc acaatcaggt cgcggatgac gggcagaccg
    11461 ggcagcgggc ggattttgac aggctgtttc aagctgcgta aatcggtcag acacgccaag
    11521 ccgtttttgc cgttgatgtt cataccgtcc gatccgcaaa tcccttcgcg gcaggagcgg
    11581 cggaaagaca aggtatcgtc ttgtgctttc aggcgtacca aagcgtccaa aagtttcacg
    11641 tcggtcggtt ccaattccaa ctcgtaacgc tgcatataag gcttggcatc aacgtccggg
    11701 ttgtaacggt aaatttcaaa actcattttt tccatgagaa gtgttccttt tctcatcaac
    11761 tgcccaatga ttttgtatta taaattcaag cagttcaatg tgggtatggt taggtttcag
    11821 acgacctttg aggcgaagac cgtctgaaaa cgcatcaata aacgcgtttg gccggtttga
    11881 tgtattccac gctcaaaggc ttggtgtgta ccggtttgta agacaaggta ttggtgtccg
    11941 aatgatacag ggtgtgtttc atccaatttt cgtcatcgcg ctcaggatgg tcgtctgaag
    12001 cgtgcgcacc gcgtgattct ttacgtgctt cggcagacac caaagtcgct ttcgccactt
    12061 caatcaggtt atccaattcc aaagcttcga tacgcgcggt attccacact ttgctcttgt
    12121 ctttgatttc ggtacgtttc acacgctcgg caatcgccat gatttcttga acgcctttgc
    12181 tcagaatctc atcggtacgg aacacgccgg cgtgcagttg tacggagcgt tgcagttcgc
    12241 gacgcaatgc atcaacgttt tcaccatcgg tttgactgtc caaacgctcg atacgttggc
    12301 gggtcaactc accggcatta gcaggcagag gtttccaatc gctttgctct ttgatgaatt
    12361 taatcatgct gtcaccggca gctttgccga acaccaccaa gtccagcagg gagttcgtac
    12421 ccaaacggtt cgcaccgtgt acggaagcac aggcgcactc acctgcggca tacaggcctt
    12481 ttacaggtac ttcgtactcg tcgccttgcg gaacaacaac ttcaccgtga taattggtcg
    12541 gaatgccgcc catcatatag tgggtagtcg gcacaaccgg aatcgggtct ttaatcggat
    12601 cgataccggc aaactgaatg gaaatctcgc ggatgcccgg cagtttttcc ataatttttt
    12661 ctgcaccgat atggtcgatt ttcagtaaga cgtggtcttt gtttttacca cagccgcgac
    12721 cttcatagat ttccatcgcc atcgcgcgtg aaaccacgtc gcgagaagcc aagtctttta
    12781 cggtcggcgc atagcgttcc ataaagcgtt cgccgtcggc gttcaacaga ataccgccct
    12841 cgccgcgtac gccttcggta atcaacacgc ccgcacccgc cacgccggtc gggtggaatt
    12901 gccagaattc catatcttcc aacggaatgc ccgcacgggc gcaaatgccc aaaccgtcac
    12961 cggtattcat ataagcattg gtagaagaag cataaatacg accgccgcca ccggtagcaa
    13021 acatcacggc cttggcgtgg aaaatataaa cttcgcccgt ttccatttcc atggcggtta
    13081 cgccgacgac atcgccgttt tcatcacgaa tcaaatcttg cgccgtccat tccacaaaga
    13141 attgtgtatt ggcacggacg ttttgttggt acaaagtatg caacatcgca tgaccggtac
    13201 ggtcggcaac cgcacatgca cgttctaccg cacgtttacc atgttcggca gtatgtccgc
    13261 cgaaaggacg ctgataaatt ttgccgcttt caacgcggtc aaaaggcata cccatgtgtt
    13321 ccaactcaat caccgcttca ggcgcagcgc gacacataaa ctcaatcgca tcttggtcac
    13381 ccagccagtc ggaacctttc acggtatcgt acatgtgcca gtcccaacgg tcctcctgca
    13441 cattacccag agaggcggaa ataccgccct gcgccgctac ggtatgcgag cgggtcggga
    13501 acactttaga caaaacggca caattcaaac cggatttgga taattggagg gctgcacgta
    13561 aacctgcacc gccaccgccg acaatcacgg catcaaactt gcgaacagga aaacccatac
    13621 ttacccccaa atcactttaa ctgaatacac gaggcagccg accagccaga caatggtggc
    13681 aacctgcaaa aacaaacgca cgccgaaggg tttgatatag tccatccaca aatcgcggat
    13741 acccacccaa gcgtgcaaga atacggcgat aaagctcact tgggtaaata cttttaccca
    13801 agcttgacta aaaaatgcct gccatgccgg atattcttta ggcagggcaa atagaaccac
    13861 taaaagtgca acggtataaa tcaacataat aaccgcagtc gcacgctgca ttacccaatc
    13921 gcgcaaaccg taatgggcac cggtcaattt acgttctacc ataacaacgc tcccaaaacg
    13981 acagtcaaaa ccaatgcaga agcaaatacg gctttagcgg tattgcgcgc agtattcagc
    14041 tcaaggcctt tgtgcgcatc caaaaataaa aagcggatac cggcgagaga atggtgcaga
    14101 taagcccaca atataccgat taaaaccagc ttgaccaaag gatgggaaac aatggcacgg
    14161 taagtttcaa atgcagactc ttgactcagg gtaccggaca gaaaatacag caggaaaggc
    14221 agcataataa acagcccgac cccgctaatg cggtgaagga tggaaactat cccgggtatc
    14281 ggcagacgga tgttcggcaa atccagataa acagggcgca gtttgacaga catagtcaat
    14341 tcctctgtag ttatatatcg actcaaaaac cgtaaaccca ttgctacact gggttacatc
    14401 aacgttaatt tacctgtttt atcccatttt gaacagtgta ttttaaaaac aaactgatag
    14461 attttgacaa acacgcgtgg aaacagcaga ttaaaagaaa gatttttagt tttgccgctc
    14521 ccttcccgac acggtatcag catccgaccc ccaaccccat ccccaaaaat aaaatgccgt
    14581 ctgaaaaaaa tttcaggcgg cattgttttg ccatatcggt caggctttca atttcgcatc
    14641 ggcatcgcgc aacgcggtac gcagaccttc ctcgataaca ggatggtaga acggcatatc
    14701 cagcatttgc ggaacggtca tcttcatttg atgcgcccat gccaacaagt gagccaaatg
    14761 ttcggcggca gggcctacga tttccgcgcc gataaagcgg ccggtggctt tttcggcata
    14821 caggcgcata tggcctttgt ttaccagcat cacgcggctg cgaccttgat ttttgaacga
    14881 tacttcgccg atgacaaatt cgtcggcttg gtattgcgcg gcaacctgcg cgtatttcaa
    14941 accgacaaag ccgatttgcg gactggtaaa caccacgcca atggtgctgc ggcgcaaacc
    15001 gctgccgata ttcgggtagc ggcccgcgtt atcgccggca atcttacctt ggtcggcggc
    15061 ttcatgcagc aggggcagtt ggttggacgc gtcgcccgca ataaagatat gcggaatgct
    15121 ggtctgcatg gtcagcggat cggcaacggg tacgccgcgc gcgtctttgt cgatattgat
    15181 gttttccaaa ccgatattgt caacgttcgg acggcgacct acggctgcca acatatattc
    15241 ggcaacaaat acgccttttt cgccatcctg ctcccaatgg acttctacat tgccgtctgc
    15301 gtcgagtttg acctcggttt tagcatccag atgcagtttc aattcttctc cgaacacggc
    15361 tttcgcctcg tctgaaacaa cggggtcgga aatgccgccg atgattccgc ccaaaccgaa
    15421 aatttcaact ttcacaccca aacggtgcaa tgcctgaccc agttccaaac cgataacgcc
    15481 cggcccgaac acggcaacgc gcttaggcag cgtatcccac gagaaaacgt cgtcgttgat
    15541 aatcaaacgg tcgcccaaag actgccactg cggcaggatg acgggacgcg aaccggtagc
    15601 aatcacgaaa ctttttgccg caatttggat atggtcgtcg atttggacgg tatgctcgtc
    15661 gatgaattta gccgagccca tgatgcgctt gtcggcaggc cattcttcca catcggtaac
    15721 gacaaagccg acaaaacggt cgcgctcgga tttgacgcgc cgcataacct cttctccgtt
    15781 gacgacgatg ctgtctttgt ccaaatgcac gccgaagggg tcggtatgca atgcgtggtg
    15841 acgcgcttct gcggcggcaa tcaagagttt ggaaggcata cagcccacgc gcgcgcaggt
    15901 tgtgccgaac acattgtttt caatcaggta aacattatcc gaatgtaaac gggcattgcg
    15961 aaacgcaccc ataccggcag taccgccgcc gattacgacg atatccgctt gaattttttt
    16021 cataatcttt gtccttccgt tttcgtctga aacattgccg acacagaaca tatcggtttc
    16081 agacagcatt taaatatctt cccaatcaaa aacattattc agataaataa aaacaaaatt
    16141 tcgattgagt gcgacaatat ccaaatcttc ttgtaaatat agagttattc ggagcggcag
    16201 tatagaaccg cccgaataac tctatccggt atatcagaca gtttaatcaa actgccttaa
    16261 gcaaacagct atcagttttt agccaagtaa gcttccaaat cttcgctgcc gccgatgtat
    16321 ttgccgccga tgaagacttg aggggcagtc atcttgccgg taatagcgcg aacggaagta
    16381 acggttgcat ctttgcccaa tacgatttct tcgtaagaca aacctttgtc ttgcaaagct
    16441 tgtttgactt tggcacagaa ttggcaacct ggtttagtga aaattgccac agactcttga
    16501 gccttccaat cgggagcaac gaatttcagc atagtatctg catcggatac tttgaaagga
    16561 tcgcccggtt cttcaggttc gatgaacatt ttttcaacca cgccgtcgtt aaccagcatg
    16621 gagtaacgcc aagagcgttt gccgaagccc aagtcttctt taccgaccag catacccata
    16681 ccttcggtaa attcgccgtt gccgtcagga atcatgtaga tgttgtctga ttcttcttcg
    16741 gcagcccaag cgttcattac gaaagtatcg tttacagata cgcagcagat tgcgtcaacg
    16801 ccgttttctt tgaacgcgcc gaataattcg ttgtaacgcg gcaggtgtga agaagaacaa
    16861 gtcggggtaa atgcaccggg cagggagaat acgactactt ttttgccttt gaacaaatca
    16921 tcggtagaca catctttcca agtgtcgccg acgcgggtgc ggaatactac ggaaggtact
    16981 ttttgaccgg tacgatcttg caaagccatt ttgagctcct taatagattg gtttgagaaa
    17041 taattgtgaa tcgccgcgca ttctacccca tgcaaaatga tttgtataat ttatagttta
    17101 aataatataa attatttaaa actatcaaat acaactccga tttgcctgtt ccccacatat
    17161 aaagccgaat tagagaaaaa caagctgcaa tgaaaagatt ttccggcaac tgctcccctg
    17221 cgccgcaact caggacaaaa tgccgtctga aaggctttca gacggcattt gttcatacgg
    17281 tctcggctta tttcgccaat tcggcacgca gtttgcgggt tacgttcatc attacttgaa
    17341 gctgttccag agtttctttc cagccgcgtg ttttcaggcc gcagtccggg ttaacccaca
    17401 gacgctcaac cggaacaacc tcaatggctt tgcgcaacag gtgttccact tcggcttctg
    17461 tcggtacgcg cgggctgtgg atgtcgtaaa cgcccgggcc gatgtcgttc gggtatttga
    17521 attcgccgaa cgcagccaag agttccatgt cggaacgtga agtctcaatg gtgatcacgt
    17581 ccgcatccat tgcagcaatc gcaggcagga tgtcgttgaa ctcagagtag cacatatgag
    17641 tgtggatttg agtgctgtct tcgcaacctg cagaggatag gcggaaggat tcgcccgccc
    17701 agttcaggta ggcatcccaa tcggcacgtt tcaaaggaag accttcgcgg atggcaggtt
    17761 cgtcaatttg gatgacttta atgccggctt tttccagatc caatacttcg tcgttcagag
    17821 ccagtgcgat ttgtttgcac acggtagcgc gcggaatatc gttgcggacg aaagaccatt
    17881 gcagaatggt tacaggaccg gtcaacatac ctttcatcgg gcgtttggtc aggttttgtg
    17941 cgtaagtaga ccaagcgaca gtcatagctt caggacggct tacgtcaccg aagatgatag
    18001 gtggtttaac gcagcgtgag ccgtagcttt gcacccagcc gtattgggtg aatgcaaaac
    18061 cgctcaacag ttcgccgaag tattcgacca tgtcgttacg ctcggcttcg ccgtgcacca
    18121 gtacgtccaa gtccagtttt tcttgctctt caaccaccaa ggcgatttct tttttcattg
    18181 cggcttcgta atcggaggca gacagttcgc cttttttgaa ggctgcgcgt gcttggcgga
    18241 tttcggtagt ttgcgggaaa gaaccaatgt tggtcgtcgg cagcagaggc aggttcaacc
    18301 atgcttgttg cgctttgata cggtcggcaa acggtgattt gcgttggtct gcgttgacag
    18361 gcaggtcggc caagcgtttg gcaacgtctg cacggtggat ttcactgctg ttggcacggg
    18421 agtcggcagc agcttggctg gcggccagtt cttcggcaac agaatcacgg ccttcattca
    18481 atgcggcttt cagaacacgc aattcttggg ttttttgcag agtgaatgcc aaccaagagt
    18541 acaggtcggg tttgttggct ttcagttttt cttcgactga caagtcaaac ggagtgtgca
    18601 gcagcgagca agagctggaa atccacaaac gctcgcccag tttggcttgc agaggcccga
    18661 cggtttccaa aactttgttc aggttggcgc gccaaatgtt gcggccgtca atcacgccgg
    18721 cagacaggac tttgtcgtag ccggcgaacg cgtccagttg ctcgggtgcg cgtaccaagt
    18781 cgatgtgcag gccgtcaaca ggcagggatt tcaacaatgc ggcgtgttcg gcaacagaac
    18841 cgaagtaagt gcccaacagg attttggcgc ttactttgtt caaagtagcg taaacgtctt
    18901 tgtatgcttc tacccattct ttaggcagat cgacagccaa agcaggctcg tcgatttgaa
    18961 tccactcggc accggcttca accaaagcag tcaggatttc aacgtaaaca ggcaacagtt
    19021 taggcaacag gctcagacgg tcgaattcaa cggagccttt ttctttaccc acccacagga
    19081 aagtcaaagg accgacaacg gtcggtttgg ctttcaagcc caaagtttgg gcttcttgca
    19141 gttgttgaac gtagtgtttg gcgttggctt tgaattcggt atcggcatgg aattcaggca
    19201 ccaggtagtg gtagttggtg tcgaaccatt tggtcatttc gatggcgaat tggtctttgt
    19261 tgccgcgcgc cagttggaag aattgttcca aaggcaggtt ttggctatcg aagccgaaac
    19321 gggcgggaat cgcgccggtg gcaacttgca ggtcgaggat gtgatcgtag aaagtgaaat
    19381 cacctacagc aacgtaatca gcgttggcag cagcttggtg tttccagttt ttctcgcgca
    19441 ggtctttggc aacgtcgagc agctcttgtt cactgatctc tttgcgccag tatttttctt
    19501 gggcgaattt caattcacgg aaggcgccga cacgcgggaa gcctgagaaa tgtaatgttg
    19561 tcatgttgaa ctctccagtt ggattttgta aggaccggtt caatccgaat cggccggtat
    19621 tgtaaatcag aatggctcgg atagggcggc gttttgcttt tcagacggcc tcggatgctg
    19681 tctgaagccg ttttcggctt taagggcgcg cgcctaaaat atggcagatg gcgtaagtca
    19741 gctcgctgcg gttgagcgtg tagaagtgga aatctttcac gccttcgcgg gacaggactt
    19801 taaccatatc gatggcgata ctggcggcga cgaggttgcg cgtgccttgg tcgtcgtcca
    19861 agccttcata catttgcgac agccatttcg ggattttgac gttggttact tgtgccattt
    19921 tgccgagttg cttgaagttg gtaacaggca ggatgccggg aacgatttcg acgtcgatgc
    19981 ccaacatcac gcagcggtcg cggaagcgca ggtagcgttc cacatcgaag aagaattggg
    20041 tgatgacgtg gttcgcgccc gcatcgattt tgcgcttcag attaatcagg tcggcttgtg
    20101 cggatttcgc ttcgggatgt acttcggggt atgctgctac agagatgtcg aagtcggcga
    20161 cggagcgtaa tagcttaacc aagtcttcgg cgtaaaacgg ttttttctca taaccggccg
    20221 gctcgtcgcc acgcagggca acgatgcggc ggataccgct gtcccaatag tctttggcga
    20281 tttggcgcaa ttcgtcgtgg gatgcatcga tgccggtcag gtgcggagct gcttccaagc
    20341 cggtttcctg tttgatgcgt ttgacgatgc cgtgcgtgcg gtcgcgttcg ccggagtttg
    20401 cgccgtaagt tacggatacg aacttgggat gcagggtttg cagacggtgg atggaatccc
    20461 acagcatcgt ttccatttgt tcgtttttcg gcgggaaaaa ttcaaacgaa acgttgatgt
    20521 tgcctttcaa atcggaaagg ctgttgttta acgcattgat ttcttttgca taatttatct
    20581 gcataccttc gttatctttt acatgatgtt tcaggcggta tgataaggtt gcaccgaata
    20641 tgagtcaatt tcaaaatttt caggtttatg atgaaaattt ttaatattta gcaaaaacta
    20701 tacgcttttc ttgtacaatc cttctaaatt atgataaagt ttgttatgtt ataacacttc
    20761 aatcaaggca atatatgaaa ccgaatattc atcctgacaa ttatcgaacc gtcctttttt
    20821 tcgacagcag tgccaatgaa ggctggctga ttcgttcttg cgccggaacg cacggaaaaa
    20881 caatggtttg gacagacggc aaagaatatc cgctcttttc cttagatact tcctcttcct
    20941 cacatcccgt ctataccggc aaacagcgta acgtcaatac cgaaggccgt gccagcaagt
    21001 tcaaccaacg tttccaatcc gtcatgtcat cgtttagaaa ggacaaataa tgcaggttct
    21061 gtcttcgctc aaaaccgcca aacaacgcca ccgcgactgc caaatcgtca gacgcagggg
    21121 caaagtgtac gtcatctgca aaagcaatcc gcgttttaaa tcgcgccaac gctgaccccc
    21181 tgcccttcct gcttcggttg taaagcccct cctaccgcaa gcccttttca aatatgccgt
    21241 ctgaaacaga aaaatgcgtt tcagacggca tttttccgtc gatacgaatc cggaatgtgt
    21301 ttgaaataca gcataaacag ataccaaaac agcgtcgcgg cggctgcacc gagcagtacc
    21361 catgcgctga tttcggcgac ttcgcgcagg acacgtttct gcctgcggct aaagaggcgg
    21421 ttgataatca gcgcggcaag tgcgaaaaag aaaaaaatac ggaaaagtct gcctatcatg
    21481 tcgggaagcg aaaagtatgg ttcgggcgcg gcggcatggg aaaggatgtt tcagcgcgtc
    21541 aggctgcttg tccgaccgtg tcaaaaagtc atgccgtttg cgatataata acgcttttcg
    21601 ccgcatttgt tggaacagga tatgagtcaa aatacctcgg tgggcattgt aacgccccaa
    21661 aaaattccgt ttgaaatgcc gctggttttg gaaaacggta aaactttgcc gcgtttcgat
    21721 ctgatgattg aaacctacgg cgagctgaat gctgaaaaaa acaatgcggt tttaatctgc
    21781 cacgcgctgt cgggcaacca tcacgttgcg ggcaggcatt cggcggagga taaatatacg
    21841 ggctggtggg acaatatggt cggtcccgga aaaccgattg atacggaacg ttttttcgtg
    21901 gtcgggttga acaatctggg cggctgcgac ggcagcagcg ggcctttgtc gatcaatcct
    21961 gaaacgggca gggaatacgg cgcggatttt ccgatggtta cggtgaagga ctgggtaaaa
    22021 tcacaagccg cgcttgccga ttatctcggc atcgaacaat gggcggcggt tgtcggcggc
    22081 agcttgggcg gcatgcaggc tttgcagtgg gcgatttcct atcccgaacg tgtgcgccac
    22141 gccttggtga ttgcgtctgc gccgaaactg tccacgcaaa atatcgcgtt taatgatgta
    22201 gcacgtcagg cgattttgac cgaccccgat ttcaatgaag gacattaccg cagccacaac
    22261 accgttcccg cgcgcggttt gcggattgcc cgtatgatgg gacacattac gtatcttgcc
    22321 gaagacggtt tgggcaaaaa attcggacgc gatttgcgtt ccaacggcta tcaatacggc
    22381 tatagcgttg aatttgaagt agaatcctat ctccgctatc aaggcgacaa attcgtcggg
    22441 cggtttgatg ctaatacata tttgctgatg accaaagctt tggactattt cgatccggcg
    22501 gcggatttcg gcaacagcct gactcgcgcc gtgcaggatg tgcaggcaaa attctttgtc
    22561 gccagcttca gcaccgactg gcgtttcgcg cccgaacgtt cgcacgaact ggtcaaggca
    22621 ctgattgccg cccaaaaatc cgtgcagtat atcgaagtca agtccgcaca cgggcacgat
    22681 gcctttttaa tggaagacga agcctatatg cgcgccgtaa cggcttatat gaacaatgtt
    22741 gacaaggatt gccgattatg aacctgcgcg atgatttgca actgatttac gaccggatac
    22801 ccgaaggcag ccgcgtctta gacttgggct gcggcgacgg cgaattgctg gcggctttgg
    22861 cggaacacaa aaaatgcagc ggctacggca tcgaaatcga caccgacaac gtgattgctg
    22921 cgatgtcgcg cggcgtaaat gttatccaag ccgatttgga agaagggttg accgcattca
    22981 acgatcaaag ttttgatgtg attgttttga gccaaaccat ccaagcgatg cagaataccg
    23041 aaaaaatcct gcgctgcctg atgcgcctcg ccaaacaggc aatcgtcagt ttcccgaatt
    23101 tcggctactg gcgcaaccgc gtccaaatcg cactcggcgg acatatgccg gtttccgaac
    23161 gtatgcccta ccattggtac gacacgccca atatccattg gtgcacgctc aaagactttg
    23221 atttgttgtg cgccaaaaac aatatccgcg tcctcgaacg cgccgtgatg acgggcaaca
    23281 gacaggtcaa acatttcccc aaccttttgg gcagccttgc gttttaccgc gtcgggtaag
    23341 acaggttgaa aaccttgtcg gcaataacgg gtttctcggt tctattaaca acagtccgtc
    23401 tgaaaaatga aacggcaggt tttcagacgg ccttttcaat attggaaaca atagaatgaa
    23461 aaacaaaacg tcatcacttc ccttatggct tgccgcaatc atgctggccg cgcgttcccc
    23521 gagcaaagaa gataaaacga aagaaaacgg cgcatccgcc gcttcgtctt ccgcgtccca
    23581 atccgatttg caaccggccg catccgcccc tgataacgtc aagcaggcag aaagcgcgcc
    23641 actgtgaaat tgcaccggcc tgcaccccgc cgccggcatt ggcgatctca tacagcaaat
    23701 cgccgaacac atcgactcgg actgtctgtt tgccctttcc cataacgaac tggaaacccg
    23761 tttcggctta cccggcggcg gctatgacaa catacagcgg ctgctgtttc ccgacatccg
    23821 ccctgaagat cccgactacc atcagaaaat catacaggca attgaagact tgcgttacgg
    23881 aacgcgcacc atcagccggc aggcacaaga tgccatgatg gaacaggaac gccgcctccg
    23941 agaagcgacg ctgctgctga cacaaggcag tcaagaaacc cgcggacaag gcgaggaacc
    24001 gaaacgcgca cgttattttg aagtttcggc aacatctgcc tatttgaacc ggcacaacaa
    24061 cggactcggc ggcaatttcc aatacatcgg ccaattgccc ggctatctga aaatgcacgg
    24121 agaaatgctt gaaaaccaat cactcttccg gctgtccaac cgtgaacgca atcccgacaa
    24181 accgttttta gacatccatt ttgacgaaaa tggcaaaatc acgcgtattg tcgtttacga
    24241 aaaaaatatc tatagtggat taaatttaaa tcaggacaag gcgacgaagc cgaagacagt
    24301 acagatagta cggcaaggcg aggcaacgct gtactggttt aaatttaatc cactatactt
    24361 caatccaaac acggggcgaa tataaaaaaa tgccgtctga aagcctttca gacggcattt
    24421 ttcaagtagg atatcaggcg cgtttgcgga attcgccggt acgggtatcg atttcgactt
    24481 tgtcgccgtt ttcgatgtaa gacatcactt ggatttcggt gccgccgacc agacgcgcgg
    24541 ttttcatcac tttgccggaa gtatcgcctt tgacggcagg ctcggtgtag tcgacttcgc
    24601 gcacgatgat ggtgggcagt tcgacagaaa tgggattgcc ttcatagaag gtaacttcgc
    24661 attggtcttc cataccgtca acgatgaatt tcaacgcgtc gccgatgttg tcggcttcga
    24721 tttcgtattg gttgaattct tcgtccataa agacgtacat cgggtcggca aaatagctgt
    24781 acgtacagtt tttgcgggac aggatgacca cgtcgaattt gtcgtcggct ttgtaaatgg
    24841 tttcggaagc agcgccggtc agcaggtttt tcagtttcat gctgactttg gcggaagagc
    24901 ggccgccttt gatgtattcg gttttttgaa cgaccatagg atcgttgccg accataaata
    24961 cattgccggc gcgcagttct tgtgctgttt tcattgtgat tttccaatca aatcggttgt
    25021 cgtaaaacgc gctattctag cgtatttttt gatgctttga aacaaaggcg gcgagttttt
    25081 cggatgcgga aggctgcccg aaaagataac ggctccaatc ctccgcgcct tgccgccagc
    25141 cgttttgatg ttgttgcagg gtttgccaac attcgaggcg ttgtgttgcg gataaagcct
    25201 ctccgccgtt gaggtcgtcc gaaaggaggc ggtgcaccga tgcggtttcg ggcgtgtaga
    25261 agccgtatgc cttatcccaa aaggcgtgga gtttgtcgag atggacattc tcgtcttgcg
    25321 ggtagatgtg ccaaaaaaag ggttttccgg caagctgggt acgcacgaaa ctgtcttcgc
    25381 cgcgtatcac ggcgcagtcg gcgaggtgca gcaatttgtc gaagtcctgt tgcggcacga
    25441 acgggatttt gacaaggcgg acggatgccg tctgaaagac accgccgtca ttttgcaggg
    25501 cgttttgcgg aataacgccg ctttgtttga ggctgtcgat aatttgcgcc cccgccagca
    25561 gtagggtcat caggctgcct gcctgttgcc acatgtccag ccactttgcc caaacatcgc
    25621 cccgatagcc gaaaagcagc cattcggggg cgtttttttc gggcagcacc agccgccggc
    25681 gcagggcttc ggtatcgaaa cggacggctt cgcggtaatc gcgttcgcgt atcaacccgc
    25741 cgcttttttc gctgaaaccc ataaaccaaa aatatttttg aacgccctcc tgcggcgaag
    25801 gcatcaggtg cagcctttca ttgctttcct ccgcgctcaa atattcccaa ttcagccaaa
    25861 gcggtttgtg tcggcggatg atgttcagca cattttcggg caggtcgcag gcaaaagttt
    25921 cgataacggc atcgggaacg ggcgcggtat caatgtctgc cgcatcggaa tgccaagtgc
    25981 ggacatgaat atcctgatga acgaagggaa catcgggcaa atcgggacaa agcgcgcgca
    26041 aggcggacac gtcgtccgtc cacaaatgca cctgccaacc gagttcgcgg tgcaaaacac
    26101 gggcgagccg ccacgaaacg ccgatgtcgc cgaaattgtc gatgaccttg caaaaaatcc
    26161 aacagacagg aaaagcgtat gtattcataa ccatcgggaa aacaagctaa aacgcgccga
    26221 aatagtggaa caaatacaca accagcaaaa caatcggaat aaacagaaac atggtttgac
    26281 gtttcaaatc aaacccttgt ttaagatgat gcttgatggt ttcaaagagg acgaatttaa
    26341 acaaaaacgt cgcttcgcag gcaaggatga tgccgttgat cagatgctgg ggtttgtctt
    26401 cgggcggatt ggcggcgaaa acggcaagcg tcgccaaggg gatgaggcag acggcgagcc
    26461 agcggacaag caggtttcgg gacattgcaa ggctttcaaa aaagcaaaac gccattatcg
    26521 cctcaagaac gcaccttgtc taacggacgc gccggagatg ccgtctgaag ccttgccgcc
    26581 ggaaaacgcc gcaccaccga acctttaaat atacccggga cgggcgttcc ccgagaaatc
    26641 ggacgctgcc gccgatgcct gcggacaagg cggcatcggg cgcgggcata ttgaaagccc
    26701 gacgcatcgg tttgaacgca ttttataaag gcgtacggca aacccgcccc ttgccgtcgg
    26761 cgtgcttccg caaagcaaaa tataagggcg gcgcgctttc cgctataatc cgcatttttc
    26821 caaacctgaa acattatgcc gaccccgaaa gcctttaccc tgctcgggcc gaccgcctgc
    26881 ggcaaaaccg cgctcgccct caaaattgcc gaaacccttc cggtcgaaat catcagcctc
    26941 gattctgcac tgctctatac cggtatggac atcggcacgg cgaaaccgtc cgcctccgaa
    27001 cgcgcctttg tcccgcacca ccttatcgac atcattacgc ccgttcagac atacagtgcc
    27061 gcccgttttg tcgaagactg cacgcgtctg accggcgaaa tcacggcgcg tgggaaatgt
    27121 ccgctgattg tcggcggcac aatgatgtat ttccgcgccc tgacccaagg tttgaacgat
    27181 ttgcccgaag ccgatgcctg cctgcgtgcc gacttggacg aacaaaaaca gatgtacggt
    27241 ttggattttc tctaccgcac cctgcaaaaa gtcgaccctg aaacagcatg ccgtctgaaa
    27301 ccgaacgaca gccagcgcat cggacgcgcc cttgaagtct attacctgac cggcaggccg
    27361 atgagcgcgc acctgaacgg acagcccgaa cacaccctgc ccttcgaact ctacaccgcc
    27421 gccctgattc ccgaaaaccg cgcccgactg cacgaaaaca tcgccctgcg tttccacctg
    27481 atgcttgaac agggctttat cggcgaagta gaaaacctgc gccgccgcta tcccggcctg
    27541 accgccgact cccccgccat ccgctgcgtc ggctaccgtc aggcgtggga acacctcgac
    27601 ggtgcaaccg acagacaaac cttcatcgaa aaaggcattg ccgccacgcg ccaacttgcc
    27661 aaacgccaac tgacttggtt acgcaaaaca cctttagact gcgttgccga cccgttttca
    27721 gacggcactt caggcacgcg cctgattgag gcggcaaaac ggtttttcgg cgaataaacc
    27781 atgctggcca cgccgccgct cgcccccaaa accgtcgccg ccctgcacag gctcggtatc
    27841 cgtacgctcg aagaactgcg gcaaaacggt tcggtcaaag cctttctgct gctcaaagca
    27901 tcgggtttga ccctcacgaa aagcacgctg tggcaactcg agtcgctgct gaacggcacg
    27961 ccgccgcaag aaatgtccca agcgcataaa gactgcctgc ttgccgagtt gaaaaaccat
    28021 ccgcccgtcg ccgcctttcc gccgcaggaa gaaatgggat acttcatgcg cgaagcactg
    28081 cgccaagcag aacaatccgc cgccgacggc gaaatacccg tcggggcagt catcgtttca
    28141 gacggcaaaa tcatcacatc ggcacacaac acctgcattg ccgactgcaa tgtcagccga
    28201 cacgccgaaa tcaacgcctt ggcacaggca ggcagcgaaa tgcaaaacta ccgccttgac
    28261 ggatgcgaca tctacatcac cctcgaaccc tgcgccatgt gcgcgtccgc actgatacag
    28321 gcacgaatca ggcgcgtgat ctacggtgcg gcagaaccca aaaccggcgc ggcaggcagc
    28381 atcgtcaacc tgtttgccga caaacgcctc aatacgcaca ccgccatacg gggcggaatc
    28441 ctgcaagaag aatgccgcgc cgtattaagc cgttttttcc aaaacaaaag aaaaggttga
    28501 acccatgacc gccctactcg tcatcctcgc cctcgccctg atagccgtcg gcacggcagg
    28561 catcgtctat cccgccctgc ccggcttggc attgatgttt gccggaacat ggctgcttgc
    28621 ctatgccggc ggctatcaaa tctacggcgc aggcatcttg tggacggtcg gactcatcag
    28681 ccttggcggc atactggcgg actatatggc aggcatgttg ggggtaaaat acactggggc
    28741 aggcaaactc gccgtccgag gtgcattggc cggcagcatc atcggcatat ttttctccct
    28801 gcccgggctg atactcggcc cctttatcgg cgcggcggca ggcgaactga tcgatcggcg
    28861 caatatgctt caggcaggta aagcgggctt gggtacgctg ttggggcttg tcgtcggcac
    28921 ggcgttcaaa atcggctgcg ccgtatccat cttgcttatc ctgttggtga aatacatcgc
    28981 ctacctgttt taaccccttc agacggcata ccggcaaatt gctataatgc cgtctgaaat
    29041 ttccgacaaa gcaccattat ggcaaaccaa agacccatct acggcttcca cgccgtcaac
    29101 gcccgattgt ggcaaaaccc caaatcgatt accgaactct atatccaaga aggcaaatcc
    29161 gacgcacgca cgcgcgaagt gctggaaaag gcggcaaacg aaaacatccg cgtatatttt
    29221 gccgatgccg accgcctcaa cgccatcagc aaaggcgcgc gccatcaggg cgtggtcgga
    29281 tttatcgatg cctccaaaaa ccacgtccac ctcgaagacg tattggaaaa cctcagcgaa
    29341 ccgccgctgc tgctgatact cgacggcatc accgatccgc acaacctcgg cgcgtgcctg
    29401 cgtaccgccg acgcaatggg cgtacacgcc gtcatcgcgc cgaaagacaa aagcgcggga
    29461 ctgaacgcca ccgtcagcaa agtcgcctgc ggcgcggcgg aaaccgtccc ctacatcacc
    29521 gtaaccaacc tcgcccgcac cctgcgcgaa ttgaaagaat acggcatctg gattatcggc
    29581 accgacatag gcggcaacgc cgacctttac cattgcaatc tgcccgacag cgcggcatgg
    29641 gtgatgggca acgaaggcga cggtatgcgc cgcctcacgc gcgaacattg cgacatgctg
    29701 gtgtccatac ccatgttcgg cacggtcgaa agcatgaacg tctccgtgtc cgcaggaatg
    29761 gtattaagcg aaacccgccg gcaacgcgta ttgaaaaaca aaaaagcgta agcctcaaaa
    29821 acaatgccgt ctgaaacagg gattccgttt cagacggcat tcgttttcaa aatcagtgtt
    29881 ccaaatggtc ggtatcaaac ttgaccgccg cctcggtttt atcttccggc aagaccaaat
    29941 tcagcaagac tgccgtaatg ccgccgccgg aaatagagtt ttggaacaag acgggcaggt
    30001 ttttaaacac ttccggctca aaccccacgc ccaagcccaa accgaccaac gttgccgcaa
    30061 tcaccgcctc gcgcctgcgg atgccgtggc cgaccaaaat ccgcacgccc gcaatcgcaa
    30121 tcaggccgaa catcaaaacc atcgcgccgc ccaacagcgg actcggaatc gtcgtaaacg
    30181 cgcgtccgac aacggggaac agacccaaca gcaccaaaat cacggcaata tatttgccca
    30241 cgtggcgcga agccactccg gtcatctgaa tcacgccgtt gttttgcgca aacgtcgtca
    30301 gcggcagcga acccaaagcc gtcgcaatca ccgataccaa gccgtcagcc aacacgccgc
    30361 cgcgcaggcg tttggtgtat tcttcgcctt caatcggctg gtcggacacc attgccgttg
    30421 ccgtcaaatc gccgaccgcc tcaaacacgc ccaacaagaa aatcgcaccc gccgcaataa
    30481 atgcgtgcca gtcaaaagca aaaccatatt taaacggtac gggcagcgta accggcggca
    30541 ggttttgcag tgcggaaaaa tccaccttgc ccaaaaacag cgcgacaatg tagccggcaa
    30601 tcagaccgac cgcaatgccg ctcatccgca gcagcggatt tttcatgcag ttgaacacca
    30661 gcacaatcag cagcaccagc gaagccagcc ccaagttttc catcgagccg aacgtgccgt
    30721 ccgccttcgc gccgaagccg ccgccgaaat cggcaatacc gacgtgtacc aaactcagac
    30781 caatcagcat cacaaccacg ccgctgaccg tcggcgtaat cacttttttc aaatacggca
    30841 gaagccacgc cgagaaacac accaaaaacg caccggcaaa cgatacgccc aagagcgtcg
    30901 aaaccatcgc cccctcactc aaaccgccct ctttcatccc cgcgccgagc gcaatcatga
    30961 cggtaacgct ggatggacag catccccgaa ccgaccggcc cgaagcggtt gacctgcaaa
    31021 taagtgccga cacccgacgc aaccatcgcc atcgacacga gatacgccgt catttccacc
    31081 ggtagttcca gcgcgccgcc cacaatcagc gcgggcgtaa tcatcggcac gaaaatcgcc
    31141 aaaagatggg taaccgcgct caagagcgca ttaccgaacg gcggcctgtc ttccaaaccg
    31201 tacaccaaat caggcgaatc cgcctgtttt ttcattgttt cagccattgg agtctttcta
    31261 tccgttgcca aacgttaaaa aattttaaga acgaaattgt aacgaatcaa agattcctta
    31321 tcaattcaaa tcctcatgtg caaaaacacc accggttgtc cgaattcaaa tttctttgaa
    31381 tttccgcacc ggacagtcct tcatttccag agagccttca tgcgcttaac acattttaat
    31441 cctcaacggc gcatccactg cttaaaccca ataatttcat gattataaaa aaaatattat
    31501 ttattaagat atgatgggac ttaagccttg gaaccaaccg tgcggccgat atgtttcaca
    31561 caaacaacaa agtaagctac aatccgaagc ataatcgacc tcactcaatg aataaaaggt
    31621 ttccgtatgt tacaaggtag cttggttgcc ctgattaccc cgatgaatca agacggcagc
    31681 atccattacg accaactccg ccaattaatc gactggcaca tcgaaaacgg cacggacggc
    31741 atcgtcg
//
LOCUS       DAWTQS010000018        30294 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.018, whole genome shotgun sequence.
ACCESSION   DAWTQS010000018 DAWTQS010000000
VERSION     DAWTQS010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 30294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30294
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.018"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(114..1256)
                     /locus_tag="WNA25_000830"
     CDS             complement(114..1256)
                     /locus_tag="WNA25_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFA9823626.1"
                     /translation="MKKSVLAVLAALSLAACGGSEKNAVQPQAGSAPAANAEAAATDT
                     LNIYNWSNYVDESTVEDFKKANNLKLTYDLYENNETLEAKMLTGKSGYDLVVPGIAFL
                     PRQIEAGAYQKVNKDLIPNYKNIDPELLKMLETADPGNQYAVPYFSGVNTVAITAKGK
                     ELLGGKLPENGWDLLFKPEYTRKLKSCGIALWDTPSEMFPILLNYLGKDPKGSNPEDL
                     KAAAEVLKSIRPDVKRFSPSIIDELARGDICLAAGNGGDLNLAKARSEEVKNNVGIEV
                     LTPKGMGFWIESWLIPADAKNVANAHKYINYTLDPEIAAKNGIAVTFAPASKPAREKM
                     PAELVNTRSIFPNEQDMKDGFVMPQMSTDAKKLSVSLWQKIKVGTN"
     gene            1560..1823
                     /gene="rpsT"
                     /locus_tag="WNA25_000831"
     CDS             1560..1823
                     /gene="rpsT"
                     /locus_tag="WNA25_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFA9823627.1"
                     /translation="MANSAQARKRARQSVKQRAHNASLRTAFRTAVKKVLKAVEAGDK
                     AAAQAVYQESVKVIDRIADKGVFHKNKAARHKSRLSAKVKALA"
     gene            1983..3131
                     /locus_tag="WNA25_000832"
     CDS             1983..3131
                     /locus_tag="WNA25_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFA9823628.1"
                     /translation="MPTMGAEMNTRNMRYILLTGLLPTASAFGETALQCAALTDNVTR
                     LACYDRIFAAQLPSSAGQEGQESKAVLNLTETVRSSLDKGEAVIVVEKGGDALPADSA
                     GETADIYTPLSLMYDLDKNDLRGLLGVREHNPMYLMPFWYNNSPNYAPSSPTRGTTVQ
                     EKFGQQKRAETKLQVSFKSKIAENLFKTRADLWFGYTQRSDWQIYNQGRKSAPFRNTD
                     YKPEIFLTQPVKADLPFGGRLRMLGAGFVHQSNGQSRPESRSWNRIYAMAGMEWGKLT
                     VIPRVWVRAFDQSGDKNDNPDIADYMGYGDVKLQYRLNDRQNVYSVLRYNPKTGYGAI
                     EAAYTFPIKGKLKGVVRGFHGYGESLIDYNHKQNGIGIGLMFNDWDGI"
     gene            3273..3974
                     /locus_tag="WNA25_000833"
     CDS             3273..3974
                     /locus_tag="WNA25_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HFA9823629.1"
                     /translation="MTEPAAEGGKAAKALKKYLITGILVWLPIAVTVWVVSYIVSASD
                     QLVNLLPKQWRPQYVLGFNIPGLGVIVAIAVLFVTGLFAANVLGRQILAAWDSLLGRI
                     PVVKSIYSSVKKVSESLLSDSSRSFKTPVLVPFPQSGIWTIAFVSGQVSNAVKAALPQ
                     DGDYLSVYVPTTPNPTGGYYIMVKKSDVRELDMSVDEALKYVISLGMVIPDDLPVKTL
                     AGPMPPEKAELPEQQ"
     gene            4032..5840
                     /gene="aspS"
                     /locus_tag="WNA25_000834"
     CDS             4032..5840
                     /gene="aspS"
                     /locus_tag="WNA25_000834"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFA9823630.1"
                     /translation="MRTNYCGLISEQYLGQTVTVKGWVHRRRDHGGVIFIDLRDREGI
                     VQVVIDPDTPEAFAAADSARNEYVLGITGRVRNRPEGTTNDKMISGKIEILAKEIEVL
                     NAAATPPFQIDDENISENVRLTNRVIDLRRPVMQRNLRLRYQVATGVRRYLDAQGFID
                     IETPMLTRSTPEGARDYLVPSRVHPGEFFALPQSPQLFKQLLMVAGFDRYYQITKCFR
                     DEDLRADRQPEFTQIDLETSFLNEDEIMDITEGMAKQVFKDALNVDLGDFPRMPYSEA
                     MFYYGSDKPDMRINLKFTELTDLMKTEEFKVFRGAADMKGGRVVALRVPNGAKFSRKE
                     IDEYTKFVGIYGAKGLAYIKVNDAGNLSNGEDSGLQSPIVKFLSENALKEIIERTAAQ
                     NGDIIFFGADKAKVVNEAVGALRIKVGLEHGKDNGYFTDEWKPLWVVDFPMFEYDEEA
                     DRYVAVHHPFTAPKEGHEDLMVSDPANCLARAYDMVLNGWEIGGGSIRIHRADVQEKV
                     FAALKISPEEQQEKFGFLLDNLKFGAPPHGGLAFGLDRLVTLMTGAESIRDVIAFPKT
                     QRAQCLLTDAPNSVDDKQLRELSLRLRQKAVETKEA"
     gene            5990..6274
                     /locus_tag="WNA25_000835"
     CDS             5990..6274
                     /locus_tag="WNA25_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFA9823631.1"
                     /translation="MKKLLLAAVVSLNAATAFAGDSAERQIYGDPHFEQNRTKAVKML
                     EQRGYQVYDVDADDYWGKPVLEVEAYKDGREYDIVLSYPDLKIIKEQLDR"
     gene            6441..8333
                     /gene="speA"
                     /locus_tag="WNA25_000836"
     CDS             6441..8333
                     /gene="speA"
                     /locus_tag="WNA25_000836"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFA9823632.1"
                     /translation="MPILTIREVCNINHWSIGYYDVDDSGEIIVRPNPSQHNQTVSLQ
                     KLTEAVQQKHQARLPVLFCFPQILEHRLRDINRAFQTAREECGYKGGYCLVYPIKVNQ
                     HRRVIESLMSSGQPHGLEAGSKAELMAVLAHAGTRQTLIVCNGYKDREYIRFALMGEK
                     LGHQVYLVIEKLSEIQMVLEEAEKLGIKPRLGVRARLASQGSGKWQSSGGEKSKFGLS
                     ASQVLQLVDILKQKNRLDCLQLLHFHLGSQLGNIRDVATGVHESARFYVELHKLGVNI
                     RCFDVGGGLGVDYEGNRTQSDCSVNYSLNEYAATVVWGISQACLEHGLPHPTIITESG
                     RGITAHHAVLVANVIGVERYKPRRLDAPSPEAPRVLHSMWETWTDISASREKRSLRSW
                     IHEGQFDLADVHNQYNVGLLSLAQRAWAEQLYLNICHEVGELFNEKHRSHRTIIDELQ
                     ERFADKLYVNFSLFQSLPDAWGIDQLFPVCPITGLNEPIARRAVLLDITCDSDGTIDH
                     YIDGDGIAGTMPMPDYPEEEPPFLGFFMVGAYQEILGNMHNLFGDTATADVVVREDGQ
                     FTVIDYDEGNTVADMLEYVYQDPKELMKRYREQIEHSDLPASQAMSFLKELEAGLNGY
                     TYLEDE"
     gene            8432..9355
                     /gene="speB"
                     /locus_tag="WNA25_000837"
     CDS             8432..9355
                     /gene="speB"
                     /locus_tag="WNA25_000837"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFA9823633.1"
                     /translation="MQYSTLAGQTDNSLVSNNFGFLRLPLNFMPYESHADWVITGVPY
                     DMAVSGRSGARFGPEAIRRASVNLAWEHRRFPWTFDVRERLNIIDCGDLVFSFGDSRD
                     FVEKMEAHAGKLLSFGKRCLSLGGDHFITLPLLRAHARYFGKLALIHFDAHTDTYDNG
                     SEYDHGTMFYTAPKEGLIDPSRSVQIGIRTEHSKKLPFTVLSAPKVNEDSVEETVRKI
                     KETVGNMPVYLTFDIDCLDPSFAPGTGTPVCGGLSSDRALKILRGLTDLDIVGMDVVE
                     VAPSYDQSDITALAGATIALEMLYLQGAKKD"
     gene            9553..11019
                     /locus_tag="WNA25_000838"
     CDS             9553..11019
                     /locus_tag="WNA25_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HFA9823634.1"
                     /translation="MMKLGFKPIPLAIAAVLCALVLALPVPDGVKPQAWTLLAMFVGV
                     IAAIIGKVMPLGALSIIAVGLVAVTGVTADKPGAAMSDALSAFANPLIWLIAIAVMIS
                     RGLLKTGLGMRIGYLFIAVFGRKTLGIGYSLALSELLLAPVTPSNTARGGGIIHPIMQ
                     SIAGSYGSNPAKGTEGKMGKYLALVNYHSNPISSAMFITATAPNPLIVNLIAENLGSS
                     FRLSWGAWAWAMAVPGVIAFFVMPLILYFLYPPEIKETPNAVQFAKDRLSEMGKMSAD
                     EIIMAVIFGILLLLWADVPALITGNHAFSINATATAFIGLSLLLLSGVLTWDDVLKEK
                     SAWDTIIWFGALIMMAAFLNKLGLIKWFSGVLAESVGGLGVSGTAAGVILVLAYMYAH
                     YMFASTTAHITAMFGAFLAAAVSLNAPAMPTALMMAAASNIMMTLTHYATGTSPVIFG
                     SGYTTMGEWWKAGFVMSVVNFLIFSVIGSIWWKVLGYW"
     gene            complement(11223..12299)
                     /locus_tag="WNA25_000839"
     CDS             complement(11223..12299)
                     /locus_tag="WNA25_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFA9823635.1"
                     /translation="MICRKPIGHTPYFDMNKTICRTAALLISGFSYANTVIPDVSPVA
                     QGQHVFINIPQQRLFLYTDGKLTKVYPVAVGRAMTQTNLGEHKIGAKAYNPVWHIPKS
                     IQKERGDGVKTIAAGPDNPLGPVFVRLGDPKLGLGIHGTNAPASVPGIRSHGCVRMKS
                     PDALEFAKTIATGSPASVIYQMAGLNEDADRNLWLAAFRDPYGKNNLDTASLKKSIGQ
                     WAKTQGKTIAPEKVDAVLKDRTGSAVCLTCGKNGKMKMPLKSLAWIQGSSSYSQAEVI
                     EQTEETNSAEVSETRTPEVPDVHTPEAQAHLNTQSDGTPTAYTEPAADSSPQVESPDQ
                     AASEPVDVLFSIDVIRQGNLRLGN"
     gene            12363..12500
                     /locus_tag="WNA25_000840"
     CDS             12363..12500
                     /locus_tag="WNA25_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase"
                     /protein_id="HFA9823636.1"
                     /translation="MATETNIAEISALDYEGRGVAKVGGKTIFIKGALLDCLMLGCFR
                     L"
     gene            12522..13664
                     /gene="bioF"
                     /locus_tag="WNA25_000841"
     CDS             12522..13664
                     /gene="bioF"
                     /locus_tag="WNA25_000841"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFA9823637.1"
                     /translation="MKVFRQQLEQLGAQNQYRSIPDLIHQGRYITRENRKMLNMSSND
                     YLGLASDENLRRSFLQQYGGNFPSFTSSSSRLLTGNFPIYTDLEELVAQRFQRESALL
                     FNSGYHANLGILPALTTTKSLILADKFVHASMIDGIRLSRCAFFRYRHNDYEHLKNLL
                     EKNVGKFDRTFIVTESVFSMDGDVADLKQLVQLKKQFPNTYLYVDEAHAIGVYGQNGL
                     GIAERDNLIAEIDLLVGTFGKALASVGAYAVCNQVLKECLINQMRPLIFSTALPPFNV
                     AWTYFIFERLPQFSKERSHLEQLSAFLRREVAHRTQIMPSETCIVPYILGGNEATLAK
                     AEYLQGQGYYCLPIRPPTVPKNTSRIRLSLTADMTTDEVRQFAACL"
     gene            13674..14321
                     /locus_tag="WNA25_000842"
     CDS             13674..14321
                     /locus_tag="WNA25_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF452 family protein"
                     /protein_id="HFA9823638.1"
                     /translation="MKTKFYNHQGEHLILYFAGWGMPPDAVNHLILPENHDLLICYDY
                     QDLNLDFDFSAYRHIRLVAWSMGVWAAERALQGIRLKSATAVNGTGLPCDDNFGIPCA
                     VFKGTLENLTENTRSKFERRICGDKASFEDYQQFPARPFDEIHQELTALFAMIGQDRR
                     TDLIRWTNALFGSGDKIFIPANQHRYWTPRCTVQETDGGHYLFSRFTHWSALWNH"
     gene            14309..15091
                     /gene="bioC"
                     /locus_tag="WNA25_000843"
     CDS             14309..15091
                     /gene="bioC"
                     /locus_tag="WNA25_000843"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFA9823639.1"
                     /translation="MESLTAINKPRIRQAFQKALSDYDRHALIQQKMTINLIAHLQDY
                     LPDMPLENVLELGCGSGMLSALLQKQISANYWLFNDLCDVRSRLAEKLPQSFDFYCGD
                     AENFPFQRQFDLIASASAVQWFHQPDAFIAHCKTGLKTNGLLAVATFGKDNLKEVRQI
                     TNIGLNYPTLSQWQAWLAKDFELLWCEDFKVILDFDTPLDVLKHLKYTGVTATNQKNW
                     TRKNLNGFIGDYLSAFGMPSGKVRLTYHPLFFIARHSAAGRQ"
     gene            15333..16770
                     /locus_tag="WNA25_000844"
                     /pseudo
     CDS             15333..16770
                     /locus_tag="WNA25_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243324.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase"
     gene            16773..17871
                     /locus_tag="WNA25_000845"
                     /pseudo
     CDS             16773..17871
                     /locus_tag="WNA25_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815828.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            17883..18418
                     /locus_tag="WNA25_000846"
                     /pseudo
     CDS             17883..18418
                     /locus_tag="WNA25_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212573.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
     gene            18420..19062
                     /locus_tag="WNA25_000847"
                     /pseudo
     CDS             18420..19062
                     /locus_tag="WNA25_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232884.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
     gene            19083..19442
                     /locus_tag="WNA25_000848"
     CDS             19083..19442
                     /locus_tag="WNA25_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HFA9823640.1"
                     /translation="MQQLLISILEDLNNTSTDIIASAVISTDGLPMATMLPSHLNSDR
                     VGAISATLLALGSRSVQELACGELEQVMIKGKSVYILLSQAGKDAVLVLVAKETGRLG
                     LILLDAKRAARHIAEAI"
     gene            19534..19609
                     /locus_tag="WNA25_000849"
     tRNA            19534..19609
                     /locus_tag="WNA25_000849"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19567..19569,aa:Asn,seq:gtt)
     gene            complement(20107..21018)
                     /gene="crgA"
                     /locus_tag="WNA25_000850"
     CDS             complement(20107..21018)
                     /gene="crgA"
                     /locus_tag="WNA25_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator CrgA"
                     /protein_id="HFA9823641.1"
                     /translation="MKTNSEELTVFVQVVESGSFSRAAEQLEMANSAVSRIVKRLEEK
                     LGVNLLNRTTRQLNLTEEGAQYFRRAQRILQEMAAAETEMLAVHEVPQGVLRVDSAMP
                     MVLHLLAPLAAKFNERYPHIRLSLVSSEGYINLIERKVDIALRAGELDDSGLRARHLF
                     DSHFRVVASPEYLAKHGTPQSAEDLANHQCLGFTEPGSLNTWAVLDAQGNPYKISPHF
                     TASSGEILRSLCLSSCGIACLSDFLVDNDITEGKLIPLFAEQTSNKTHPFNAVYYSDK
                     AVNLRLRVFLDFLVKELGKNMNRTNTK"
     gene            21257..21835
                     /locus_tag="WNA25_000851"
     CDS             21257..21835
                     /locus_tag="WNA25_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFA9823642.1"
                     /translation="MNILLLDGGKAFGHSHGGLNHTLHKKAKEVLTALGHNVQETVID
                     AGYDVEAEIEKFVWMDAVIWQMPGWWMHEPWTVKKYMDEVFTGGHGKLYQSDGRHRVN
                     PTEGYGTGGLLQGKKHMLSLTWNAPIEAFTREGDFFEGKGVDVLYMHFHKANEFIGLS
                     RLPTFLCNDVVKNPQVEKYLADYQAHLEKVFG"
     gene            complement(22043..23428)
                     /gene="pntB"
                     /locus_tag="WNA25_000852"
     CDS             complement(22043..23428)
                     /gene="pntB"
                     /locus_tag="WNA25_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFA9823643.1"
                     /translation="MSSGLVTAAYIVAAILFIFSLAGLSKQETAKRGCYSGIAGMAVA
                     LFVTVFSDNTHGLGWIIIAMLIGAAIGIHKAKKVEMTEMPELIALLHSFVGLAAVLVG
                     FNSYIEPGNVSHDMHTIHLVEVYLGIFIGAVTFTGSLAAFGKLNGKISSSPLQLPAKH
                     KLNALALAVSFVLLLVFVGIDGSGFILLIMTLIALAFGWHLVASIGGADMPVVVSMLN
                     SYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFVSVIAGGFGSDSG
                     ISSSGSQEIEEYREVKAADVAEMLKGASSVIITPGYGMAVAQAQYPVAEITELLRKNG
                     IEVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFPETDVVLVIGANDTVN
                     PAAQTDPNSPIAGMPVLEVWKAKEVVVFKRSMNTGYAGVQNPLFFNENSVMCFGDAKK
                     TVDGILAELKK"
     gene            complement(23508..23891)
                     /locus_tag="WNA25_000853"
     CDS             complement(23508..23891)
                     /locus_tag="WNA25_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFA9823644.1"
                     /translation="MTFAYWCILIACLLPLFCAAYAKKAGGFRFKDNHNPRGFLAHTQ
                     GAAARAHAAQQNGFEAFAPFAAAVLTAHATGNAGQATVNTLAGLFILFRLAFIWCYIA
                     DKAALRSLMWAGGFACTVGLFVAAA"
     gene            complement(23895..25433)
                     /locus_tag="WNA25_000854"
     CDS             complement(23895..25433)
                     /locus_tag="WNA25_000854"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFA9823645.1"
                     /translation="MKIGIPRESLSGETRVACTPATVALLGKLGFETVVESGAGLAAS
                     LDDAAYQTAGATVADKAAVWACPLIYKVNAPSEGELPLLKEGQTIVSFLWPRQNEALV
                     EALRAKKVNALAMDMVPRISRAQALDALSSMANISGYRAVIEAANAFGRFFTGQITAA
                     GKVPPAQVLVIGAGVAGLAAIGTANSLGAVVRAFDTRLEVAEQIESMGGKFLKLDFLQ
                     ESGGSGDGCAKVMSDEFIAAEMKLFAEQAKEVDIIITTAAIPGKPAPKLITKEMVESM
                     KSGSVIVDLAATGGNCELTRPGELSVTGNGVKIIGYTDMANRLAGQSSQLYATNLVNL
                     TKLLSPNKDGEITLDFEDVIIRNMTVTRDGEITFPPPPIQVSARPQQTPSEKAAPAAK
                     PEPKPVPLWKKLAPAAIAAVLVLWVGAVAPAAFLNHFIVFVLACVIGYHVVWNVSHSL
                     HTPLMSVTNAISGIIVVGALLQIGQGNGFVSLLSFVAILIAGINIFGGFAVTRRMLNM
                     FKKG"
     gene            25722..26555
                     /gene="serB"
                     /locus_tag="WNA25_000855"
     CDS             25722..26555
                     /gene="serB"
                     /locus_tag="WNA25_000855"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HFA9823646.1"
                     /translation="MPQALVLQFPSAEALPSDFPSRLPEPDYADEKRMRFIVEEGFSL
                     SEKDAALLDSRQIDHAVLPNMAFGELGLIVSDMDSTLITIECIDEIAAGVGLKGKVAE
                     ITERAMRGELDFEQSLRSRVALLAGLDEQILADIYENVLKLSPGAEFLLDECKRHNVK
                     FLLVSGGFTFFTERLQQRLGFEYQHANILEIENGRLTGRLKGRIIDAQAKADLLREYR
                     SRLGLQPHQVLAMGDGANDIPILKEAGIGVVYRAKPKARAAADACINFGGLERVRGLF
                     G"
     gene            26619..27842
                     /locus_tag="WNA25_000856"
     CDS             26619..27842
                     /locus_tag="WNA25_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HFA9823647.1"
                     /translation="MGRRKLWFALAAAGVIGGLVGIVLTELMHFIRHTAYGYDADGAY
                     ISFREGVAQAFGMRRVAMLTLCGAIAGGGWWLLKRFGKPQIEIKAALKQPLQGLPFLT
                     TVFHVLLQIITVGLGSPLGREVAPREMTAAFAFAGGRRLGLDEGEMRLLIACASGAGL
                     AAVYNVPLASTLFILEAMLGVWTQQAVAAALLTSVIATAVARIGLGDVQQYHPANLAV
                     NTSLLWFSAVIGPILGATAAFFRRTAQKFPFIKRDNIKIIPLAVCMFALIGVIAVWFP
                     EILGNGKAGNQLTFGGLTGWQYSLELTAVKWAVVLMALAAGAYGGLITPSMMLGSTIS
                     FAAAAAWNSVFPEMPSESAAVVGAAVFLGVSLKMPLTAIVFVLELTYAPLSLLLPLCI
                     GMAGALGTSGKMGFK"
     gene            28003..29583
                     /gene="purH"
                     /locus_tag="WNA25_000857"
     CDS             28003..29583
                     /gene="purH"
                     /locus_tag="WNA25_000857"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFA9823648.1"
                     /translation="MSVIKRALISLSDKAGAVEFAQNLHKLGVEILSTGGTAKLLAGA
                     GVPVIEVADYTGFPEMLDGRVKTLHPKIHGGILGRRDLDEHVAKMEEHGIGNIDLVCV
                     NLYPFAATIAKPGCTLEDAIENIDIGGPAMVRSAAKNWKHVAIVTDTADFPAIAAELE
                     AGNGALSDKTRFNLSRKAFSHTAQYDGMISNYLTSLSDDVLSGQPQIGEFPSQFNQSW
                     IKVQDMRYGENPHQRAAFYRDIDPAAGSLSAYNQLQGKELSYNNIADADAAWEAVKSF
                     DAPACVIVKHANPCGVAVAADTLTAYKLAYATDTTSAFGGIIAFNREVDGETVKQITD
                     NQFMEVLMAPKFTAEALEIAAAKKNVRVLEVPLKAGANRFELKRVGGGLLVQTPDINR
                     INRADLKVVSKRQPTEQEWNDLLFVWNVAKYVKSNAIVFGKGGQTYGIGAGQMSRVDS
                     TRIAARKAQDAGLDLNGACAASDAFFPFRDGVDVIAEQGIKAIIHPAGSMRDQEVFDA
                     ADEHGIAMAVTGIRHFRH"
BASE COUNT         7413 a         7396 c         7917 g         7568 t
ORIGIN      
        1 atgtccgcaa tctgaaatcc cggccgtggg ggcgacggta taaatatttc tatttccatc
       61 caatataaaa tgccgtccga acatcgttcg gacggcattt ttaatgcttc gaatcagttg
      121 gtgccgactt tgattttctg ccacaggctg accgacagtt ttttcgcatc cgtgctcatt
      181 tgcggcatga cgaaaccgtc tttcatatcc tgctcgttcg ggaagatgga acgggtgttc
      241 accaactcgg caggcatttt ttcgcgcgcc ggtttgctgg cgggggcgaa ggttacggcg
      301 atgccgtttt tcgccgcgat ttcggggtcg agcgtgtagt tgatgtattt gtgggcgttg
      361 gcgacgtttt tcgcatcggc gggaatcagc caagactcaa tccagaagcc catacctttc
      421 ggtgtcagca cttcgatgcc gacgttgttt ttcacttctt cggaacgcgc tttcgccaag
      481 ttcaaatcgc cgccgttgcc tgccgccagg cagatgtcgc cgcgcgccag ctcgtcgatg
      541 atggacgggc tgaaacgttt gacgtccgga cggatagact tcaacacttc cgccgccgcc
      601 ttcaaatctt caggattcga acctttgggg tctttgccca agtagttcag caaaatcggg
      661 aacatttcgc tcggggtgtc ccacaaggcg atgccgcagg atttcagctt gcgggtgtat
      721 tcgggtttga acagcaaatc ccagccgttt tcgggcagct tgccgcccaa aagctctttg
      781 cccttcgccg taatcgcaac cgtgttcacg ccggagaaat aggggacggc atactggttg
      841 cccgggtcgg cggtttccag cattttcaag agttcgggat cgatgttttt atagttggga
      901 atcaggtctt tgttgacttt ttgatacgcg cccgcctcga tttggcgcgg caggaaggcg
      961 atgcccggca caaccaaatc gtaaccggat ttgccggtca gcattttggc ttccagcgtt
     1021 tcgttgtttt cgtacaagtc gtaagtcagc ttcagattgt tggctttttt aaagtcttcg
     1081 accgtactct cgtcaacata gttcgaccag ttgtagatgt tcagagtatc ggtggcagcg
     1141 gcttcggcat tggcagcagg tgcgctgcct gcttgaggct gcacggcgtt tttttcgctg
     1201 ccgccgcagg ctgccagaga cagcgcggcc aaaacggcta atacggattt tttcatacgg
     1261 gcagattcct gatgaaagag gttggaaaaa aagaaaaccc cgcgccccat agacaccccg
     1321 gcgcaaggtt tgggcattgt aaagtaaatt tgtgcaaact caaagcgata ttggcctgat
     1381 tttcctaaaa aattaccctg tttccaaaaa agggggggaa acggccgccc gattttgccg
     1441 tttttttgcg ccgtcagggt gtccgacggg cggatagaga aaaaaggctt gcatataatg
     1501 taaaccccct ttaaaattgc gcgtttacag aatttatttt tctttcagga gattccaata
     1561 tggcaaacag cgcacaagca cgcaaacgtg cccgccagtc cgtcaaacaa cgcgcccaca
     1621 acgctagcct gcgtaccgca ttccgcaccg cagtgaaaaa agtattgaaa gcagtcgaag
     1681 caggcgataa agctgccgca caagcggttt accaagagtc cgtcaaagtc atcgaccgca
     1741 tcgccgacaa aggcgtgttt cataaaaaca aagcggctcg ccacaaaagc cgtctgtctg
     1801 caaaagtaaa agcactggct tgatttttgc aaaaccgcca aggcggttga tacgcgataa
     1861 gcggaaaacc ctgaagcccg acggtttcgg ggttttctgt atttcggggg taaagttcga
     1921 aatggcggaa agggtgcggt tttttatccg aatccgctat aaaatgccgt ttgaaaacca
     1981 atatgccgac aatgggggcg gagatgaata cacgaaatat gcgctatatt cttttgacag
     2041 gactgttgcc gacggcatcc gcttttggag agaccgcgct gcaatgcgcc gctttgacgg
     2101 acaatgttac gcgtttggcg tgttacgaca ggatttttgc ggcacagctt ccgtcttcgg
     2161 cagggcagga agggcaggag tcgaaagccg tactcaatct gacggaaacc gtccgcagca
     2221 gcttggataa gggcgaggcg gtcattgttg ttgaaaaagg cggggatgcg cttcctgccg
     2281 acagtgcggg cgaaaccgcc gatatctata cgcctttgag cctgatgtac gacttggaca
     2341 aaaacgattt gcgcgggctg ttgggcgtac gcgaacacaa tccgatgtac cttatgccgt
     2401 tttggtataa caattcgccc aactatgccc cgagttcgcc gacgcgcggt acgactgtac
     2461 aggaaaaatt cggacagcag aaacgtgcgg aaaccaaatt gcaggtttcg ttcaaaagca
     2521 aaattgccga aaatttgttt aaaacccgcg cggatctgtg gttcggctac acccaaagat
     2581 ccgattggca gatttacaac caaggcagga aatccgcgcc gttccgcaat acggattaca
     2641 aacctgaaat tttcctgacc cagcctgtga aggcggattt gccgttcggc ggcaggctgc
     2701 gtatgctcgg tgcgggtttt gtccaccagt ccaacggaca gagccgtccc gaatcgcgtt
     2761 cgtggaacag gatttatgcc atggcaggca tggaatgggg caaattgacg gtgattccgc
     2821 gcgtgtgggt gcgtgcgttc gatcagagcg gcgataaaaa cgacaatccc gatattgccg
     2881 actatatggg gtatggcgac gtgaagctgc agtaccgcct gaacgacagg cagaatgtgt
     2941 attccgtatt gcgctacaac cccaaaacgg gctacggcgc gattgaagcc gcctacacgt
     3001 ttccgattaa gggcaaactc aaaggcgtgg tacgcggatt ccacggttac ggcgagagcc
     3061 tgatcgacta caaccacaag cagaacggta tcggtatcgg gttgatgttc aacgactggg
     3121 acggcatctg aataccgttt caaacggcat atcaagttgc cgtgccgtct gaagccgccg
     3181 gcggtttggc ggggcgggat aaaaaattcg tttgaatccg ggcaaacgcg tataatgtgc
     3241 ggtttgtacg gactttgtcg gaatcaagca agatgacgga acctgcggcc gaaggcggca
     3301 aagctgccaa ggcgttaaaa aaatatctga ttacaggcat tttggtctgg ctgccgattg
     3361 cggtaacggt ttgggtggtt tcctatatcg tttccgcgtc cgaccagctt gtcaacctgc
     3421 tgccgaagca atggcggccg caatatgttt tggggtttaa tatccccggg ctcggcgtta
     3481 ttgttgccat tgccgtattg tttgtaaccg ggttatttgc cgcaaacgtg ttgggccggc
     3541 agattcttgc cgcgtgggac agcctgttgg gtcggattcc ggttgtcaaa tccatctatt
     3601 cgagtgtgaa aaaagtatcc gaatcgctgc tgtccgacag cagccgttcg tttaaaacgc
     3661 cggtactcgt gccgtttccc caatcgggta tttggacaat cgcattcgtg tccggtcagg
     3721 tgtcgaatgc ggttaaggcc gcattgccgc aggatggcga ttatctttcc gtgtatgtcc
     3781 cgaccacgcc caacccgacc ggcggttact atattatggt aaagaaaagc gatgtgcgcg
     3841 aactcgatat gagcgtggac gaagcgttga aatatgtgat ttcgctgggt atggtcatcc
     3901 ctgacgacct gcccgtcaaa acattggcag gacctatgcc gcctgaaaag gcggagttgc
     3961 ccgaacaaca ataaagccgc cgttcagacg gcattttccg ttttcagttt aaatcaataa
     4021 aaggtgattt tatgcgtacc aactattgcg gcctgatcag cgagcaatac ttaggccaaa
     4081 ccgttaccgt caaaggctgg gtacaccgcc ggcgcgacca cggcggtgtg atttttatcg
     4141 acctgcgcga ccgcgaaggc atcgtccaag tcgtgatcga tcccgacacg cccgaagcgt
     4201 ttgccgctgc cgattccgcc cgtaacgaat acgttttggg cattaccggc cgcgtacgca
     4261 accgtcccga aggcacgacc aacgataaaa tgatttccgg caaaatcgag attcttgcca
     4321 aagaaatcga agtgttgaac gccgccgcta cgccgccgtt ccaaatcgac gacgaaaaca
     4381 tcagcgaaaa cgtgcgcctg accaaccgcg ttatcgacct gcgccgccct gtgatgcagc
     4441 gcaacctgcg cctgcgttac caagtcgcta cgggcgttcg ccgttatctg gacgcgcaag
     4501 gcttcatcga catcgaaacg ccgatgctga cccgctccac tccggaaggc gcgcgcgact
     4561 acctcgtacc gagccgcgtt catccgggcg agtttttcgc gctgccgcaa tcgccgcaat
     4621 tattcaaaca actgttgatg gtggcgggtt tcgaccgtta ctaccaaatc accaagtgct
     4681 tccgtgacga agacttgcgc gccgaccgcc aacctgaatt cactcaaatc gacttggaaa
     4741 cctcgttcct gaacgaggat gaaatcatgg atatcactga aggcatggcc aaacaagtct
     4801 tcaaagatgc tttgaacgta gatttgggcg acttcccacg catgccttat tctgaagcca
     4861 tgttctacta cggctctgac aaaccggaca tgcgcatcaa cttaaaattc accgagttga
     4921 ccgacctgat gaaaacggaa gaattcaaag tcttccgtgg cgcagccgac atgaaaggcg
     4981 gccgcgtagt cgctctgcgc gtgcctaacg gcgccaaatt cagccgcaaa gaaatcgacg
     5041 aatacactaa atttgtcggc atctacggcg cgaaaggtct ggcatacatc aaagtaaacg
     5101 atgcgggcaa cctctccaac ggcgaagaca gcggcctgca atctccaatc gtaaaattct
     5161 tgtctgaaaa cgccctgaaa gaaatcatcg agcgtaccgc cgcgcaaaac ggcgacatca
     5221 tcttcttcgg tgcggacaaa gccaaagtcg tgaacgaagc cgtcggcgca ttgcgtatca
     5281 aagtcggctt ggaacacggc aaagacaacg gctacttcac agacgaatgg aaacctttgt
     5341 gggttgtcga tttcccgatg ttcgaatacg acgaagaagc cgaccgctac gttgccgtac
     5401 accatccgtt taccgctcct aaagaaggtc atgaagacct gatggtttcc gatcctgcaa
     5461 actgcctggc ccgtgcctac gatatggtat tgaacggctg ggaaatcggc ggaggctcta
     5521 tccgtatcca ccgtgcagac gtacaagaga aagtgtttgc cgcgctgaaa atcagccctg
     5581 aagagcaaca agagaaattc ggcttcctct tggacaacct gaaattcggt gcgcctcctc
     5641 acggcggcct tgcattcggc ctcgaccgtc tggtaacgct gatgaccggt gccgaatcca
     5701 tccgcgacgt gattgccttc ccgaaaacac aacgcgccca atgcctgctg accgatgcgc
     5761 ccaacagcgt ggacgataag cagttgcgtg aattgagcct gcgtctgcgt caaaaagcag
     5821 ttgaaacaaa agaagcataa ggaaaacaga gccgtttgac ggctctgttt tttcagacgg
     5881 catttacgct tcttgacttc cctctaattc aaacctaatt ttgctgtgtt ttaatggcgg
     5941 tattgaaaaa cacatcttgt tcaatcaacc gataaaaaaa ggactgaaaa tgaaaaaact
     6001 gttattggcc gccgttgttt ccctaaatgc cgcaaccgca tttgccggcg actctgccga
     6061 gcgtcagatt tacggcgatc cccattttga acaaaaccgc acaaaagccg tgaaaatgtt
     6121 ggaacagcgc ggttatcagg tttacgatgt cgatgccgac gactactggg gcaaacctgt
     6181 tttggaagtg gaagcctata aagacggccg cgaatacgac atcgtgttgt cttaccccga
     6241 cctgaaaatc atcaaagagc agctcgatcg ctgactcctt tgatggaaag atgaaccaaa
     6301 atgccgtctg aagcgttcag acggcatttt gcctgttcct catcaggtat gaggcaggct
     6361 tttcttatta aaaaaatgac atttcacgct gatttgttat aatcattcct tttcaacacg
     6421 acagacggag caggtttatt atgcctatcc ttaccatccg tgaagtgtgc aacattaatc
     6481 attggagcat aggttattat gatgttgacg attccggcga aatcatcgtc cgccccaatc
     6541 cctcgcaaca caatcaaact gtttcactgc aaaaactgac tgaagccgtg caacaaaaac
     6601 atcaggcgcg cctgcctgtt ttgttttgtt ttccgcaaat cctcgaacac cgcctccgcg
     6661 acattaaccg cgcctttcag acggcacggg aagagtgcgg ctataagggc ggttattgtt
     6721 tggtttaccc tatcaaggtc aaccaacacc gccgcgtcat cgagtcgctt atgtcaagcg
     6781 gacaaccgca tggtttggaa gccggttcta aagccgaact gatggcggtt ttggcacacg
     6841 ccggcacccg gcaaacatta atcgtctgca acggctataa agaccgtgaa tatatccgtt
     6901 tcgccttgat gggcgaaaaa ctggggcatc aggtttattt ggtgattgag aagctgtccg
     6961 aaatacaaat ggtattggaa gaggcggaaa aactcggcat caagccccgt ttgggtgtgc
     7021 gcgccagact ggcttcccaa ggttcgggaa aatggcagtc ttcgggtggg gaaaaatcaa
     7081 aattcggctt gtcggcttcc caagttttgc aactggtcga tattttgaaa caaaaaaaca
     7141 ggctggactg cctgcagctt ttgcatttcc atttgggctc gcagcttggg aacatccgtg
     7201 atgttgccac aggtgtacac gaatcggctc ggttttatgt tgagttgcac aaactggggg
     7261 taaatatccg ctgttttgat gtaggtggcg ggcttggcgt ggattacgaa ggaaaccgca
     7321 cacaatcgga ttgttccgtt aattacagcc tgaacgaata tgccgccaca gtcgtatggg
     7381 gcatcagtca ggcttgtctc gaacacgggc tgccgcatcc gacaatcatt accgagagcg
     7441 ggcgcggcat taccgcacat cacgccgttt tggttgctaa tgttataggc gttgaacgtt
     7501 acaaaccgcg tcggctggat gcgccatcgc ccgaagcacc gcgtgtgttg cacagtatgt
     7561 gggaaacttg gacggatatt tccgcctcgc gggaaaaacg ttccttacgc agctggatac
     7621 acgaagggca gtttgatctt gccgatgtgc ataatcagta taatgtcggg ctgttgagtt
     7681 tggcgcaacg tgcgtgggcg gagcaactgt atttaaatat ctgtcatgaa gtcggcgaat
     7741 tgtttaatga aaaacaccgg tctcaccgaa ccattattga cgaattgcaa gaacgttttg
     7801 ccgataagct gtatgtcaat ttctcactct tccaatcttt gcccgatgct tggggcatag
     7861 atcaactttt ccctgtttgt cctattaccg gtttgaatga accgattgcg cgccgcgccg
     7921 tgttgttgga cattacctgc gattcagacg gtacgattga ccactacatc gacggcgacg
     7981 gcatcgccgg tacgatgcct atgcctgatt atcccgaaga agagccgccg tttttaggct
     8041 tttttatggt gggagcatat caggaaatac tcggcaatat gcacaatctt ttcggcgaca
     8101 ctgccactgc tgatgttgtt gtaagggaag acggacaatt taccgtcatc gattacgatg
     8161 aaggaaacac cgttgccgat atgctcgaat acgtttatca agatccgaaa gagctgatga
     8221 aacgctatcg cgaacaaatc gaacattcag accttcctgc ctcgcaggct atgtctttct
     8281 taaaagaact cgaagcgggg cttaatggtt atacctattt ggaagacgaa tagacgcatc
     8341 aaggtatcgg atatgtcgtc tgaagcccga ttttcttact caaacaccaa tcatcacgac
     8401 cgattgaaac caattacaag gaatcattac gatgcaatac agcacactgg caggacaaac
     8461 cgacaactcc ctcgtttcca ataatttcgg gtttttgcgc ctgccgctta attttatgcc
     8521 gtatgaaagc catgccgatt gggttattac cggcgtgcct tatgatatgg cggtttcagg
     8581 gcgttccggc gcgcgtttcg gtcctgaagc catccggcgc gcctccgtca acctcgcttg
     8641 ggagcaccgc aggtttccgt ggacatttga tgtgcgcgaa cgcctgaaca ttattgattg
     8701 cggcgacttg gttttttctt ttggcgacag cagggatttt gtcgaaaaaa tggaagcgca
     8761 cgccggcaaa ttactttctt tcggcaaacg ctgtttgagt ttgggcggcg accatttcat
     8821 taccctcccg ttgttgcgcg cccacgcccg ctatttcggc aaactcgcac tgattcattt
     8881 tgacgcgcac accgacacct acgacaacgg cagcgaatac gaccacggca cgatgtttta
     8941 taccgccccc aaggaaggcc tcatcgaccc gtcccgttcc gtacaaatcg gcatacgcac
     9001 cgaacacagt aaaaaattgc cttttactgt gttgtccgcc cccaaagtca atgaagacag
     9061 tgttgaagag accgtccgta aaatcaaaga aaccgtcggc aatatgcccg tttacctgac
     9121 tttcgacata gactgtctcg acccgtcgtt cgcccccggg accggtacgc ccgtatgcgg
     9181 cggcttgagc agcgacaggg cattaaaaat cctacgtggg ctgacggatc tcgacatcgt
     9241 cggtatggat gttgtagaag ttgccccctc ttacgaccaa tccgacatta ccgctttggc
     9301 cggcgccaca attgccttgg aaatgcttta ccttcaaggt gcgaaaaagg actgaacgtc
     9361 cggcatcccc cgggttttcg ccgtgccgtt caaacggcgt attcagtcta atgaaaattc
     9421 aaatactgaa acaaaagttg cccggagccg catatcggaa agacggtgaa atatcgtaat
     9481 ataacttata aaacaattag ttaaatatta tttttccgat ttttcgggac ggtctttttt
     9541 acggaggtca atatgatgaa attgggtttc aaaccgatac ccctcgccat tgccgcagta
     9601 ttgtgcgccc tggttttggc actgcccgta cccgacgggg tcaagcctca ggcttggacg
     9661 ctgctggcta tgtttgtcgg tgtgattgcc gccattatcg gcaaggttat gccgttgggc
     9721 gcgctgtcga ttattgccgt cgggttggtc gcagtaaccg gcgtaaccgc cgacaaaccg
     9781 ggcgcggcga tgagcgatgc gttgagtgcg ttcgccaatc cgttgatttg gctgattgcc
     9841 atcgcagtta tgatttcgcg cggtttgctc aaaacagggc tggggatgcg tatcggatat
     9901 ttgtttatcg ccgtttttgg aagaaaaacg ctgggcatcg gttacagtct cgctctttcc
     9961 gaactgctgc tggctcccgt taccccttcc aataccgcgc gcggcggcgg cattatacat
    10021 ccgattatgc agtcgattgc cggcagttac ggctccaatc ccgcaaaagg cacagaaggc
    10081 aagatgggta aatatttggc tttggtcaat tatcattcca atcccatttc gtcggctatg
    10141 tttattactg caactgcccc caacccttta atcgtcaact tgattgccga aaatttaggc
    10201 agtagtttcc gtctttcttg gggggcgtgg gcgtgggcaa tggctgttcc cggcgttatc
    10261 gcctttttcg ttatgccttt gattttatat tttttgtatc cgcctgaaat taaagaaacg
    10321 cccaatgctg ttcaatttgc caaagaccgt ctgagcgaga tgggtaaaat gtcggcagac
    10381 gaaatcatta tggcggtcat tttcggtatc ttgctgctgt tgtgggcaga tgttcccgcc
    10441 cttattaccg gcaatcacgc ttttagtatc aacgccaccg ccaccgcatt tatcggatta
    10501 agcctgcttt tgctttccgg tgtattgact tgggacgatg ttttgaaaga aaaaagcgcg
    10561 tgggatacga ttatttggtt tggcgcattg attatgatgg ccgcattttt aaataaactc
    10621 ggactgatta aatggttctc cggagtgttg gcggaaagtg tcggcggttt gggcgttagc
    10681 ggcacggctg cgggcgtaat cctcgtgctt gcttatatgt atgcgcatta tatgtttgcc
    10741 agtactactg cacatattac cgctatgttc ggcgcatttc tcgctgctgc cgtttcactg
    10801 aatgccccgg cgatgccgac cgcgctgatg atggcggccg catccaacat tatgatgacc
    10861 ctcactcatt atgcgaccgg tacttcacct gtgattttcg gctcgggcta caccacaatg
    10921 ggagaatggt ggaaggcggg ttttgtcatg agcgtagtca attttctgat tttttccgtt
    10981 atcggcagca tttggtggaa agttctggga tattggtaaa gggaaaaata aatttccaat
    11041 ctgtgtttat ttgattgggc gactattatc gtgaaatatg ccgtctaaag ccttcagacg
    11101 gcatatttgt gcgcttggat gttgcagaaa gcggcagggc ggcggtgtag gaaaagccaa
    11161 acaaaaacca aaccgcctgt caacttctga taaacataag tattaaataa tcagaaggtt
    11221 attcaattac ctaaacgcaa attcccctgc cgtatcacat ctattgaaaa taatacatca
    11281 accggctcgg aagcagcctg atcaggtgat tctacttgcg gcgatgaatc ggcagccggt
    11341 tcggtatagg cagtcggcgt gccgtcggat tgggtattta aatgcgcttg tgcctcgggc
    11401 gtgtgtacat caggcacttc gggcgtgcgt gtctcggata cttcggcaga gttggtttcc
    11461 tcagtttgtt caatgacttc agcttggctg tatgaggaag aaccctgtat ccacgccagc
    11521 gatttgagcg gcatcttcat cttgccgttt ttgccgcagg tcagacagac ggccgatccg
    11581 gtgcggtctt tgagtacggc atcgactttc tcgggcgcga tggttttgcc ctgtgttttt
    11641 gcccattgcc cgatgctttt tttcagggag gcggtgtcaa ggttgttctt accgtaaggg
    11701 tcgcggaagg cggcaagcca caggttgcga tccgcatctt cattgaggcc cgccatttga
    11761 tagatgacgg aggcgggcga gccggtggcg atggttttgg caaactcgag cgcgtcgggc
    11821 gatttcatgc ggacgcagcc gtgactccgg atgcccggga cgctggccgg tgcattggtt
    11881 ccgtgtatgc ccaaaccgag tttcgggtct cctaagcgga caaaaaccgg ccccaaaggg
    11941 ttgtccgggc cggcggctat ggtttttacg ccgtcgccgc gttctttctg tatggatttt
    12001 gggatgtgcc aaacagggtt ataggctttc gccccgattt tatgttcgcc tagattggtt
    12061 tgtgtcatcg cccgacctac tgcaacggga taaaccttgg tcagtttgcc gtcggtgtag
    12121 aggaacaggc gttgctgagg gatgttaatg aagacatgtt gaccttgtgc gacgggggag
    12181 acatcgggaa tgacggtgtt tgcgtatgaa aaaccgctta tcaatagtgc ggcagtgcgg
    12241 cagattgttt tattcatatc aaaatatggt gtgtgtccga taggttttcg gcaaatcata
    12301 cctgaaaccg taccaatttg tgcgaaaata tgcgcttcgg tacagtgcgg acggattggg
    12361 taatggcaac ggaaacaaat atcgcggaaa tttccgcctt ggattatgaa ggcaggggtg
    12421 tggcaaaggt cggcggcaaa acgattttca ttaaaggggc attacttgat tgtttgatgc
    12481 tgggttgctt caggctttaa ctcaggaata tttacatcat aatgaaagtt tttagacaac
    12541 agcttgaaca actcggcgcg caaaatcaat atcgttcgat tccggatttg attcatcaag
    12601 ggcggtatat tacgcgggaa aaccgcaaaa tgctgaatat gtcgtctaat gattatttgg
    12661 ggttggcatc agatgaaaac ttacgccggt cttttttgca gcaatacggc ggtaattttc
    12721 cctcttttac cagttcttca tcacgtttat taacgggcaa ctttcctatt tataccgatt
    12781 tggaagagct tgtcgcacaa cgtttccaac gggaaagcgc gttattgttc aacagcggct
    12841 atcacgccaa tctcggtatt ttgcctgctt tgacgacgac gaaaagtttg attttggcag
    12901 ataaattcgt tcacgccagt atgattgacg gcatccgttt gagccggtgt gcgtttttcc
    12961 gttatcgtca taatgattat gaacatttga aaaatctgct tgaaaaaaac gtcggaaaat
    13021 ttgaccgcac ttttatcgtt accgaatctg ttttcagtat ggacggcgat gtggcggatt
    13081 tgaaacagct tgtccaatta aaaaaacagt ttcccaatac ttatctttat gtggatgaag
    13141 cccacgcaat cggtgtttat gggcaaaacg gattggggat tgccgaacgg gataatctga
    13201 ttgccgagat tgatttattg gtcggcactt tcggtaaagc cttagcctcg gtgggggcgt
    13261 atgccgtctg caaccaagta ttgaaagaat gtttgattaa tcaaatgcgc ccattgattt
    13321 tttcaaccgc attgccgccg tttaatgtgg cttggactta ttttattttt gaacgattgc
    13381 cgcaattctc aaaagaaaga agtcatcttg agcagttaag cgcattttta cggcgggaag
    13441 tggcgcatcg gacgcaaata atgccgagcg aaacctgtat cgtcccctat attttaggcg
    13501 ggaatgaagc cacccttgcc aaagcggaat atctgcaagg tcagggttat tattgcctgc
    13561 ccatcagacc gccgacagta ccaaaaaaca catccagaat ccgcctgtct ttaactgccg
    13621 atatgacaac ggatgaagtt cggcagtttg cggcgtgcct gtaaggatat gatatgaaaa
    13681 caaaatttta caatcatcaa ggcgaacatt taatcctgta ttttgcaggt tggggaatgc
    13741 cgcccgatgc tgtaaatcat ttgattttgc cggaaaatca cgatttattg atttgctatg
    13801 attatcaaga tttaaatttg gattttgatt tttccgccta tcggcacatc cgtttggtgg
    13861 cgtggtcaat gggcgtttgg gcggcagaga gggcattgca aggaataaga ttaaaatccg
    13921 caacggcagt gaatggcaca ggtttgcctt gcgatgataa tttcggtatc ccttgcgccg
    13981 tttttaaagg cacattggag aatctcacag aaaatactcg ttcaaagttt gaacgtagaa
    14041 tctgtggcga taaagcatct tttgaagatt accaacaatt tcccgcccgt ccgtttgacg
    14101 aaattcatca agaacttacc gcactttttg cgatgatcgg gcaagatagg cgtacagatc
    14161 ttatccgctg gacaaacgcc ttgttcggat cgggcgataa aatttttatt cctgccaatc
    14221 agcaccgata ttggacaccg cgttgcaccg ttcaagaaac agacggcggg cattacctgt
    14281 tttcaagatt tacccattgg tcggcactat ggaatcactg actgccataa ataaaccgcg
    14341 cattcggcag gctttccaaa aagcattaag cgattatgac aggcacgcct taatccaaca
    14401 aaaaatgacg attaatctaa tcgcgcattt gcaagattat ttgccggata tgccattgga
    14461 aaacgtgttg gaattgggct gcggctcagg aatgttgagt gccttgctgc aaaaacagat
    14521 ttcagcgaat tattggttat ttaatgattt gtgcgatgta cgaagccgac tggctgaaaa
    14581 attgcctcaa tcctttgatt tttattgcgg cgatgcggaa aactttcctt ttcaacgaca
    14641 atttgactta atcgcaagcg catctgccgt gcaatggttt caccaacccg acgcttttat
    14701 cgcccattgc aaaacaggct tgaaaacaaa cggattattg gcggttgcga cctttggcaa
    14761 agacaattta aaagaagtcc gccaaatcac aaatataggc ttaaattacc cgactttatc
    14821 ccaatggcag gcttggttag ccaaagattt tgagctttta tggtgtgagg attttaaggt
    14881 aatactagac tttgatacgc cgttagatgt actcaaacac cttaaatata caggcgttac
    14941 cgccacgaac caaaaaaatt ggacaagaaa aaatctcaat ggatttattg gcgattactt
    15001 gtcggcgttc ggtatgccgt cgggcaaagt gcgcctgact tatcatccgt tattttttat
    15061 cgcgcgccac tctgcggcgg gcaggcaata aggcagctta tgggcaaagt tatttttata
    15121 tcgggtattg atactgatgt gggtaaaagg taatatggcg agccttatgc agaaggcata
    15181 ttgtcaaacg ttaaattatg gtatgattta aaatttacaa gtctatttca gtaaatcgtt
    15241 aataataaaa gcggacaatt taataataaa agcggataat tggccgttgc aggcggtcag
    15301 ctatccgctc gttacggtat cgggggtttc ctttggaaaa ctcattaatt atcggtctgg
    15361 cactggcggt attgctgatg cttttggtta tgcgtgcgaa gcagggtaag aaaacaccga
    15421 agcgcaaatc ccaaggtgtc ggcaatacgc aggggcagcc gcccggaagc aatgattcgg
    15481 actgggtcga tcagacttgg tcaatcggtt tcagacggca cgcaactcga ctggtcttgg
    15541 aacgaaagtg ccgagaccgc atccgccgcc gtatccgcgc aagaagtcga tccgcttacg
    15601 gagtatcagg tttataagca attcggttat cagggcaagg ctgccgaatc tttggctgcc
    15661 tatctggacg gcattccgga tggtgaagcg aaacctgaga accttatccg cgagctgatc
    15721 gatatcaatc tcgaagtggg ggatgtcgat gttttggcag acaatctgca aaaatacggc
    15781 agactgattc ctttcgaact tttggcaaaa tatatcgaac aggcattaca gcgagattca
    15841 aaccatttgc gtatccgcgt cttggcggaa gaaggtttgg gatagggcac tcaggagatt
    15901 gaaaaacgtg cggaaggcgg ttctgcgacg gcagcttccg catcgccccc gccggatgtc
    15961 ggcggtaagg cttatggagc cgaagaaatc aagcgcatcc cgattgtgcg gggcaaaaaa
    16021 gacgtgtccg gaatcagcca agagaaaatc ggtgcgattg ccggcctggt ccgcgccgat
    16081 caaggtgcga aaatccttaa agacaaagtc agctacgaaa cggcatcgaa acaatacgac
    16141 cgtgccatcc aaacttccga aaaacctgca aacctgatta tcgatgcgtt gaaactcgat
    16201 taccaacacg cggacataga ccgttttgcc ggacatttgt ggaaacttta ccaaacgttg
    16261 ggcaactacg gcaggcaggt taaagagcgg atgctgggtt aggggtacag cctgggttac
    16321 catgaagttt tcgatgattt ggaaaagggg gcgaacgatc ggcaaatcaa agacatcggt
    16381 atgaggcacg ggtatctgcc gaaaaatata cagaaattca aagcgcaaca tcgggatttg
    16441 gtgcttcaag attcttcgtt gatcaacact ggttcgtctc cggcagacga tgcggttaaa
    16501 gaagtagagt cgttgctgat gtatggtcag attgaagcgg caatggatgt gttggagcag
    16561 gcggtattga aatatcccga cgaatcccag ctttatatta cgttgatcga tatttatgaa
    16621 cgtactgaag attgggatag gttggggcag tttttaaggg tattgaggga acgtgcggac
    16681 aggcttcctg aagaggtcgt tatgctgatg agccggctgc tgcagcgtat gaatcaaaat
    16741 attaaaaaaa taaaacggta cggaaaataa aaatggaagt tcaactgccg aaaattaaaa
    16801 cagtacgcgt aatgttggcg gggatgacgg cgcagcagga atccgttttc aaaatggcat
    16861 tcaaaatgca caataccacc cgttatgaaa cagtatcccc ttcagacggc agtgccgtgc
    16921 ccgatttggt tttggcggat accgatgccg agggcggttt tgaactttgg aaagagcttg
    16981 ccgggcgtta taaggctata cccgtcgccg tctgttcgga gaaagttccc gattctgaag
    17041 ttccctacct gcccaaaccg attcggtttg aaacattgtt tcctatgctc cgcaagttat
    17101 tgcagggcga gaatgtttat gggaaatcgt ttattgcacc cgcagaccgg tcggcgaaaa
    17161 acaacgggaa tgtgcagcgt acggttacga tacgccagtt taacccgaat aaaggattat
    17221 tgggggcgtt gcggtttgcg gaaaagaaca ggcaggacat cgctatcttg catggaaata
    17281 agccggtcct taattgtttt cccctcgata caacgggttt tgctgacaga aagtgtgtaa
    17341 aaactcgaag aattgtgcaa agacgaaaat ttgcaggtca gttgcaagac tgttcccgat
    17401 aactcgcaat ggcgcgaaaa ggctaaagta ggcattatgt cctgtatgtg gcagttttcc
    17461 atttggacgg cgcaggacag gttgatttat ccgatttttc ccgatactcc gtttacgttg
    17521 aaatcttggc caaacctgac ccggtaggca aatgtgccgg ggtcgatacg cttgtcggca
    17581 tttttgacca aggcatccgt caaccttaac gtgttgtata aagtgatgcc tttaaacctc
    17641 aatgatattc tgaattatct tgcggcaacc tatacaaccg ggtttttgtc ggtagatttg
    17701 aaaacggttt cacaacaggc atactccgat atggcggata aaataaatat cggggccgat
    17761 tctgcttctg atagtgaaat gatgaaaaaa gcggaaaaaa tcacgacacc atcccaatcc
    17821 cagtcgcgcg gccttctgca aagactgatg aaaaaactgt tgggcagcta agaggcggag
    17881 agatgagaga aaataaaatt attttcacag gacctgtcgg cgtagggaaa accactgcca
    17941 ttgcggctat ttcggacgaa gcactcgtcc agaccgatgc ttccgcatcc gacatgactt
    18001 tggataggaa aaggaatacg acagtggcga tggactacgg gccatcagct tggatgagga
    18061 taccaaagtc catttatata gtacgcccgg tcaggaacgg ttcaacttta tgtgggaaat
    18121 cttaagccaa ggcagtatgg gtttggtatt gcttttagat aatgcccgaa ccaactcgtt
    18181 gaaagatttg gaattctttt tacattcgtt tcgagggctg ctggagaagg catccgtcgt
    18241 tgtcggtatt accaagatgg atatacgctc tcagcccggt atcgacgtgt atcacaaata
    18301 tcttgcaaaa cataatctta atgttccggt ttttgaaatt gatgcccgta aggaagatga
    18361 cgtaaaacaa ttggttagcg caatgttatt ttctattgat ccgggactgg aggtttaata
    18421 tggaatcaac actttcacta caagcgaatt tatatccctg cctgactcct gccggtgcat
    18481 tttatgccgt atccagcgat gcccccagtg ccggtaaaac tttgttgcgc agcctgttga
    18541 aagcggatgc ggacgaagtg gtcagcagtg agaagctgct cgcgggcgga caccgccgac
    18601 atcgataccg ctttgaacct gttgtaccgt ttgcaaaaac tcgaattcct ctatggcgat
    18661 gaaaacggtc attcagacgg catcaatttg tcggacgagc aattgccgtt gctgatggaa
    18721 caattgtccg gcagcggtaa ggcattattg gtcgatcgga acggtctgta tcttgccaac
    18781 gccaatttcc atcatgagtc ggcggaagag ttggggttgt tggcggcaga agtcgcacag
    18841 atggaaaaga aataccggct gctgattagg aacaacctgt atatcaacaa taacgcttgg
    18901 ggcgtttgcg atccttccgg tcagagcgaa ttgacatttt tcccattgta tatcggttca
    18961 accaaattta ttttggttat cgccggcatt cccgatttga gcaaagaggc atttgttact
    19021 ttggtaagga ttttataccg ccgttacagc aaccgcgtgt aaaacttggg acaggaggga
    19081 ttatgcagca attattgatt tcaatccttg aagatttaaa taatacatct acggatatta
    19141 tcgcgtctgc cgttatctca accgacggat tgccgatggc gacaatgctt ccttcacatt
    19201 tgaattcgga cagggtaggg gcgatttctg ccactttgct tgctttgggg agtcgctcgg
    19261 tgcaggaact cgcctgcggg gaattggaac aagtgatgat taaaggaaaa tcagtctata
    19321 tccttttaag tcaggcgggt aaagatgccg tgttggtgct ggtggcaaaa gaaaccggca
    19381 gacttggttt aatcctattg gatgccaaac gtgcggcaag gcatattgcg gaagccatat
    19441 aacatataaa gattgcgggc ttgcagataa gtgcaatcga ttgtcaattt atattgacac
    19501 gttcggtatt tctgttttat tattcgcgtt tactccccga tagctcagtc ggtagagcga
    19561 cggactgtta atccgcaggt ccctggttcg agcccaggtc ggggagccaa attccaaaac
    19621 cctctaagta ttttcttaga gggttttgtt ttaccggcgg tcagaaacgc atttttgaga
    19681 tgattgtttt gagatggaat aaaatctttg caaaattcct ttcgtgatgg ttatgaaaaa
    19741 taaatttcta aagatttaat tggttatttt tataattttg aaatttatcg gttttgtaaa
    19801 ggtctcggga taagcaattt cggtcttcgg cgcgtctttt acgccaaacc cataagaatt
    19861 ttgagggtaa tcttcatcgt tttgcctgaa ttgccgacgc aaagcgattt atcggcggtt
    19921 gtgtttgagc gtttgattgc agcttgccca atctcaaata tttctaaata ccaattgaaa
    19981 ttcaatattt tataaaaaca ctgatttgga acgaacagat ttataaaaat tccgtgtatc
    20041 cttgagtaag ggagttgatt gaaaagactt gtttgattga tgatgaagaa taagttaatt
    20101 aatgtcttat ttggtattcg ttctattcat attttttccc agttccttca ctaaaaaatc
    20161 caaaaatacg cgtaagcgga ggttgacggc tttatcgctg taataaacag cattaaaggg
    20221 gtgtgtttta ttggaggttt gttcggcgaa taggggaatt aactttcctt cagtgatgtc
    20281 gttgtcaacc aaaaaatctg ataagcaagc aataccgcaa cttgaaaggc acaacgagcg
    20341 taagatttca ccgctgctgg cggtaaagtg cggtgaaatt ttatagggat ttccctgcgc
    20401 atctaaaacc gcccatgtat ttagagaacc gggttctgtg aagcctaaac attgatggtt
    20461 ggcaagatct tctgcagatt gtggcgtgcc gtgttttgct aaatattcag gactggcgac
    20521 tacgcggaag tggctgtcaa acagatggcg tgcacgcagc ccggaatcgt ccaattctcc
    20581 ggcccgtaag gcaatatcga ctttgcgttc aatcagattg atatagcctt cggaagaaac
    20641 gagcgaaagt cggatatgcg gatagcgttc gttgaatttt gctgccagcg gcgccagcag
    20701 atgcagcacc atcggcatcg cggaatccac gcgcaacacg ccttgcggta cttcgtgcac
    20761 tgccagcatt tcggtttccg ccgctgccat ttcttgcagg attctctgcg cgcggcggaa
    20821 atattgcgcg ccttcttccg tcagattgag ttgccgcgtg gtgcggttga gcaggttcac
    20881 gcccaacttt tcctccagcc gtttgacgat gcggcttacg gcagaatttg ccatctccaa
    20941 ctgctccgcc gcacggctga agctgccgct ttccaccact tgaacaaata cggtcagttc
    21001 ttctgaattg gttttcatcg tgtttccttt tcggttggaa ccccaccctt tagggcggca
    21061 ggatcagact ttatttggga agggcgtaac cccttcccaa tcaggacggc acatagggag
    21121 acgatttatg tgtcatcccg tgtgttgaaa catctgatta ttccatttga cgcaaaagtg
    21181 ttttcttatt tttgcacttt taaatcataa agtaaaacgg tacaatacat tcatcaattc
    21241 acaaacgagg taacaaatga atattttatt attagacggc ggcaaagcgt tcggacattc
    21301 tcacggcggg ttaaaccaca cgcttcacaa aaaagcgaaa gaagttttga ccgcgctcgg
    21361 acacaatgtt caagaaaccg tgattgatgc cggctatgat gttgaggcgg aaatcgaaaa
    21421 attcgtttgg atggatgctg tgatttggca gatgccgggc tggtggatgc acgagccttg
    21481 gacagtgaaa aaatatatgg acgaagtctt taccggcgga cacggcaaac tctaccaaag
    21541 cgacggcaga caccgcgtca atccgactga gggctacggc acaggcggct tgttgcaagg
    21601 caaaaaacat atgctttcac tgacttggaa tgcgccgatt gaagccttta cccgcgaagg
    21661 cgatttcttt gaaggcaaag gcgttgatgt tttgtatatg cacttccaca aagccaacga
    21721 gttcatcggc ttgagccgcc tgccgacatt cttatgtaac gatgtggtta aaaatccgca
    21781 agtggaaaaa tacttggcag attatcaggc acacttggaa aaagtgttcg gctaattaaa
    21841 aatccatctt caacacggag atggattttg tttgtttcgt tgattttgtg tcagtttcag
    21901 atgtgggtgc ttatccggac gggtcgtctg aaaatgtttg ccccaatgca aaaaaatcac
    21961 tgcaaacctt cacaaacggg gctggtagtg attttttcaa atcaaacaga ttgaaaacct
    22021 gcgccgaatt gttcagacgg cattattttt tcaattccgc caaaatcccg tcaacggttt
    22081 tcttcgcgtc gccgaaacac atcacgctgt tttcgttgaa gaacagcggg ttttgtacgc
    22141 ctgcgtagcc ggtgttcatc gagcgtttga atacgacgac ttcttttgcc ttccatactt
    22201 ctaaaaccgg catacctgca atcgggctgt tcggatcggt ttgggcggca gggttgacgg
    22261 tatcgttcgc accaatcacc aagaccacat cggtttcggg gaagtcgtcg ttgatttcgt
    22321 ccatttccaa aacaatgtcg taggggactt tggcttcggc gagcagtaca ttcatatgac
    22381 cgggcaggcg gccggcgacg gggtggatgc cgaagcgtac ttcgatgccg tttttacgca
    22441 aaagctcggt gatttcggca acggggtatt gtgcctgcgc cactgccatg ccgtagcccg
    22501 gggtgataat gacgctgctt gcgcctttca gcatttcggc aacatcggca gctttgactt
    22561 ctcggtattc ctctatctct tggctgccgg aagacgatat gccgctgtcg ctgccgaaac
    22621 cgccggcaat cacggaaaca aacgaccgat tcatggcttt gcacataatg taggacagaa
    22681 ttgcgccgct tgagccgacc agcgcgccgg taacgatgag caggtcgttg gagagcataa
    22741 agcctgccgc tgcggccgcc cagccggagt aggagttgag catagatacg accacgggca
    22801 tatccgcgcc gccgatggag gcaaccaagt gccagccgaa tgcgagggcg atcagggtca
    22861 taatcagaag gatgaagccg ctgccgtcaa tgccgacaaa tacgagcagc aacacaaacg
    22921 atacggcgag cgccagtgcg ttgagcttgt gtttggcggg cagttgcagc gggctgctgc
    22981 tgattttgcc gttgagtttg ccgaatgcgg ccagtgagcc ggtaaaggtt accgcgccga
    23041 tgaaaatgcc caaatagact tcgaccagat ggatggtgtg catatcgtgc gaaacgttgc
    23101 ccggctcgat gtagctgttg aagccgacca aaacggctgc caagccgacg aagctgtgca
    23161 gaagtgcaat cagttcgggc atttcggtca tttctacttt tttggctttg tggatgccga
    23221 ttgccgcgcc gatgagcatg gcgatgataa tccagcccag tccgtgggta ttgtcggaaa
    23281 agaccgttac gaagagggcg accgccatac cggcgatacc ggaatagcag ccccgtttgg
    23341 cggtttcctg tttggacagc cccgccagtg agaagatgaa taaaattgcg gcaacgatat
    23401 acgccgctgt tacgagtcct gaagacatgg aaattctccg attttcgatg atttgttttc
    23461 aatgccgtct gaaaaattga cgttcgtgtt ttcagacggc atctgtttca agcagccgcg
    23521 acaaacagtc cgacggtgca ggcaaatccg cccgcccaca tcagcgagcg caatgctgct
    23581 ttgtctgcga tgtagcacca gataaaggcg aggcggaaca ggatgaacaa tccggcaagc
    23641 gtgttgacgg ttgcttgtcc ggcattgccg gttgcgtgtg ccgtcaaaac ggcggcggca
    23701 aacggtgcaa aggcttcaaa accgttttgc tgcgcggcgt gggcacgggc ggctgcgcct
    23761 tgcgtatgtg ccagaaaacc gcgaggattg tggttgtctt taaaccggaa tccgcctgct
    23821 tttttggcat acgccgcaca aaaaagcggc aataggcagg caatcagaat gcaccaatag
    23881 gcgaaagtca tggcttaccc tttcttaaac atattcagca tacgccgtgt aaccgcaaag
    23941 ccgccgaaga tattgatgcc ggcaatcagg atggcaacaa acgacagcag cgaaacgaag
    24001 ccgttgccct gaccgatttg cagcagcgcg ccgacgacga tgatgccgga gatggcgttg
    24061 gttaccgaca tcagcggtgt gtgcagcgag tggctgacgt tccaaacgac atggtagccg
    24121 atgacgcagg cgaggacgaa gacgataaag tggttcaaga atgctgcggg tgcgaccgcg
    24181 ccgacccaca gcaccaatac ggcggcgatg gcggcgggcg cgagtttttt ccacagggga
    24241 acaggtttcg gctcgggctt ggcggcaggc gcggcttttt cagacggcgt ttgctgcggc
    24301 cgggcggaaa cctgaatcgg cggaggcggg aaggtgattt cgccgtcgcg ggtaacggtc
    24361 atattgcgga taatcacgtc ttcgaagtcc agcgtgattt cgccgtcttt gttcgggctt
    24421 aacagcttgg tcaggttcac caagttggtg gcgtaaagct gggaagactg tccggcaagg
    24481 cggtttgcca tgtcggtgta gccgatgatt ttcacgccgt tgccggttac ggacaattcg
    24541 cccggtcggg tgagttcgca gttgccgccc gtcgccgcca aatcgacgat gacggatccg
    24601 gatttcatgc tttccaccat ttctttggta atcagcttgg gagcgggttt gcccggaatg
    24661 gcggcggtgg tgatgatgat gtccacttct ttcgcctgtt cggcaaagag cttcatttcg
    24721 gcggcgataa attcgtcgct catcactttg gcgcagccgt ctccgctgcc gcccgattct
    24781 tgcaggaagt cgagtttcag gaacttaccg cccatcgatt cgatttgttc cgccacttcc
    24841 aagcgggtat cgaacgcgcg caccactgcg ccgagcgaat ttgccgtacc gattgccgcc
    24901 aaacccgcca cacctgcacc aatcaccaaa acctgcgcag gcggcacttt gccggcggca
    24961 gtgatttgac cggtgaagaa acggccgaag gcgttggcgg cttcaatcac ggcgcggtag
    25021 ccgctgatgt ttgccattga agacaaagcg tccaaggcct gagcgcggga aatgcgggga
    25081 accatgtcca tcgccagcgc gttgactttc ttggcgcgca aggcctcgac caaagcctcg
    25141 ttttggcgcg gccacaggaa gctgacgatg gtttgacctt ctttgagcag cggcagctcg
    25201 ccttcggacg gcgcgttgac cttataaatt aaagggcagg cccaaaccgc cgctttgtcg
    25261 gcaacggttg cgcctgctgt ttggtaagcg gcatcgtcca aacttgccgc caaacctgca
    25321 ccgctttcga caacggtttc aaagcctagt ttgcccagca gggcaacggt ggcgggcgtg
    25381 caggcgacgc gggtttcgcc ggataatgac tcgcgtggga taccgatttt catctctgaa
    25441 tcctttttcg ggttgttcat atgtatcgtg ggttaaattt aaatcggtgc agggcggagc
    25501 aacgccgtac cggtttaaaa ccgattcact tcaaatgtta atatatttta gataatcccc
    25561 ttataacgaa ttttcatcag gctggcaata gttgcgcatt ttcccgtgtt gtccgacaca
    25621 tactgccgtt tgcgtttgaa gtcgttttcc gattgcaggc acgcccgccg taacattctt
    25681 tgccgtttca ctatataatc cgcatttttt gagccgccgt tatgccgcaa gccctcgtcc
    25741 tccaatttcc ctccgccgaa gccctgcctt ccgacttccc ctcacgcctg cccgaacctg
    25801 attatgctga tgaaaagcgt atgcgtttta ttgttgaaga aggattttct ttaagcgaaa
    25861 aagacgcggc gttactggac agccgtcaaa tcgaccacgc cgttttgccc aatatggcgt
    25921 ttggcgaact cgggctgatt gtcagcgata tggattcgac gctgattacc atcgaatgta
    25981 tcgatgaaat tgcggcaggt gtgggtttga aaggcaaagt agcggaaatt accgagcgcg
    26041 ccatgcgcgg agagttggat ttcgaacagt ctttacgcag ccgcgttgcg ttgttggcgg
    26101 gattggacga acagattttg gcggacattt atgaaaacgt tttgaagctc tcgcccggtg
    26161 cggaattttt gttggacgaa tgcaaaaggc acaatgtgaa attcctgctg gtgtccggcg
    26221 ggttcacgtt ttttaccgaa aggctgcaac aacggctcgg cttcgaatac caacacgcca
    26281 atattttgga aattgaaaac ggcaggttga ccggccgtct gaaaggcaga atcatcgacg
    26341 cgcaggcaaa ggcggattta ttgcgcgaat accgcagccg cctcggattg cagccgcatc
    26401 aggtgttggc gatgggcgac ggtgcgaacg atattccgat actcaaagaa gcgggcatag
    26461 gcgtggttta ccgcgccaaa ccgaaagcgc gggccgccgc cgatgcctgt atcaacttcg
    26521 gcggtttgga gcgtgtacgc ggcctgttcg gataggcgga taggaaacgg atgccgtctg
    26581 aaaggttttc agacggcatt tgaacggcag gaacgacagt gggacgcaga aaactttggt
    26641 ttgccctggc ggcagcaggc gttatcggcg gtttggtcgg cattgtgctg acggaactga
    26701 tgcacttcat acggcatacg gcatacggtt atgacgcgga cggtgcgtac atctcgttcc
    26761 gcgaaggtgt ggcacaggct ttcggtatgc ggcgcgttgc catgctgacg ctgtgcggcg
    26821 cgatcgcagg cggcggctgg tggctgctca aacgtttcgg caagccgcaa atcgaaatca
    26881 aagccgcctt gaagcagccg ttgcaggggc tgccgtttct gacgacggtt ttccatgttc
    26941 tgctgcaaat cataacggtc ggactcggtt cgccgctcgg acgcgaagtc gccccgcgtg
    27001 aaatgactgc cgcgtttgct tttgccggcg gcaggcggtt gggtttggac gaaggcgaaa
    27061 tgcgcctctt gattgcctgt gcgtccggag cgggtttggc ggcggtgtat aacgtgccgc
    27121 tcgcctccac gctgtttatc ctcgaagcga tgttgggcgt gtggacgcag caggcggttg
    27181 ccgccgcatt gctgacttcc gtcatcgcca ccgccgtcgc gcgcatcggc ttaggcgacg
    27241 tgcagcagta tcatcccgcc aaccttgccg taaatacttc attgctttgg ttttccgccg
    27301 tcatcggccc gatacttggc gcaaccgccg cctttttccg gcgcaccgcc caaaaattcc
    27361 ccttcatcaa gcgcgacaat atcaaaatca ttcccttggc cgtctgtatg tttgcactca
    27421 tcggtgtgat tgccgtttgg tttcccgaaa ttttgggcaa cggcaaagca ggcaaccaac
    27481 tgacttttgg cggtttgacc ggctggcaat acagccttga gttgaccgct gtcaaatggg
    27541 cggtcgtttt gatggcgctg gcggcaggcg catacggcgg actgattacg ccgtctatga
    27601 tgctcggcag caccatttct tttgcagccg ccgccgcgtg gaacagcgtc ttccccgaaa
    27661 tgccgtctga aagcgcggca gtcgtcggag cggcggtgtt tctcggcgta tcgctcaaaa
    27721 tgccgctgac cgccattgtg tttgtattgg aactgaccta cgcgccgctg tccctgctgc
    27781 tgcctttgtg catcggcatg gcgggggcat tgggaacatc gggaaaaatg ggcttcaaat
    27841 gaatgccgac aaatgccgtc tgaaaggttt ccagacggca tttctttgca aattgtcaac
    27901 aatatctgca aaaacctact gccaaaaaac tgaaaatagc ggataatacc gccccgaaaa
    27961 agatcccctt tcaccatcca accgtcagaa aaggaagccc caatgtccgt catcaaacgc
    28021 gccctgatca gcctgtccga caaggcaggc gcggttgaat ttgcccaaaa cctgcacaag
    28081 ctcggtgtcg aaatcctctc caccggcggc acggcgaaac tgcttgccgg tgcgggcgtt
    28141 cccgtgattg aagtcgccga ctacaccggt tttcccgaaa tgctcgacgg ccgcgtgaaa
    28201 accctgcatc cgaaaatcca cggcggtatt ttgggccggc gcgatttgga cgaacacgtc
    28261 gccaagatgg aagagcacgg catcggcaac atcgaccttg tgtgtgtcaa tctttacccc
    28321 ttcgccgcca ccatcgccaa gcccggctgc acactggaag acgcaattga aaacatcgac
    28381 atcggcggcc cggccatggt gcgttccgcc gcgaaaaact ggaaacacgt cgccatcgtt
    28441 accgacaccg ccgacttccc cgccattgcc gccgaactcg aagccggcaa cggcgcgttg
    28501 agcgataaaa cccgcttcaa cctctcacgc aaagcattca gccacaccgc ccaatacgac
    28561 ggcatgattt ccaactacct gacctccctt tcagacgacg ttttgagcgg ccagccccaa
    28621 atcggcgaat tcccaagcca gttcaaccaa agctggatta aagtgcaaga catgcgctac
    28681 ggcgaaaacc cgcatcagcg cgccgcgttt taccgcgata ttgaccccgc cgcaggcagc
    28741 ctttccgcct acaatcaact gcaaggcaaa gaattgtctt acaacaacat cgccgatgcc
    28801 gatgccgctt gggaagccgt caaatccttt gacgcgcccg cctgcgtgat cgtgaaacac
    28861 gccaatccgt gcggcgtagc ggttgcagcc gataccttga ccgcctacaa actcgcctac
    28921 gccaccgaca ccaccagcgc gttcggcggc atcatcgcct tcaaccgcga agtcgacggc
    28981 gaaaccgtca aacagattac cgacaaccaa tttatggaag tcctcatggc gccgaagttt
    29041 accgccgaag ccctcgaaat cgccgccgcc aagaaaaacg tgcgcgtatt ggaagtgccg
    29101 ctcaaagcag gtgccaaccg cttcgaactc aaacgcgtcg gcggcggact gttggtacaa
    29161 acccccgaca tcaaccgcat caaccgcgcc gatttgaaag tcgtctccaa acgccaaccg
    29221 accgagcagg aatggaacga tttgctgttt gtctggaacg tcgccaaata cgtcaaatcc
    29281 aacgccatcg tgttcggcaa aggcggtcaa acctacggca tcggcgcagg ccaaatgagc
    29341 cgcgtggaca gtacccgcat cgccgcccgc aaagcgcaag atgccggtct cgacctcaac
    29401 ggcgcatgtg ccgcttccga tgccttcttc ccattccgcg acggcgtgga tgtcattgcc
    29461 gagcagggca tcaaagccat catccatccg gcaggctcga tgcgcgatca ggaagttttc
    29521 gacgcggcgg acgaacacgg catcgccatg gcggtaaccg gcatccgcca cttccgccat
    29581 taaggcagat gaataaggca atgccgtctg aagggttttc agacggcatt ttgcgccatt
    29641 taccgtaatc ctgaaatccc gtcattccca cgcaggcagg aatctagatg agaatccatc
    29701 ctgtcagaaa tacccgaatc gtcagaaata cccgtgtcat cattcccgat acaccgtaat
    29761 cctgaaaccc gtcattcccg cgcagacaga aatccggatt tgtccgcgcg gaaacttatg
    29821 cgccgccatt cccgcgaagg cgggaatccg gaccgttcgg tttcggttat ttccgataaa
    29881 ttcctgctgc ttttcattcc tagattccca ctttcgcggg aatgacgaag gagtgggaat
    29941 ccggtttttg agttccggcc atttccgaca aattgctttg gcattggata tttcctattt
    30001 taatccacta tagtggatta actttaaacc agtacggcgt tgcctcgcct tagctcaaag
    30061 agaacgattc cctaaggtgc tgaagcacca agtgaatcgg ttccgtacta tttgtactgt
    30121 ctgcggcttc gccgccttgt cctgattttt gttaatccgc catatccgat cgccgcaccg
    30181 gaattttaaa aaataaatac attctttaac aaaaaaaaat catatcccgg caaaacaaat
    30241 ctgataaaat acttggcgtt tattaacgat ctttttaata atttaatctt agat
//
LOCUS       DAWTQS010000019        28334 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.019, whole genome shotgun sequence.
ACCESSION   DAWTQS010000019 DAWTQS010000000
VERSION     DAWTQS010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 28334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28334
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.019"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(130..681)
                     /locus_tag="WNA25_000858"
     CDS             complement(130..681)
                     /locus_tag="WNA25_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823649.1"
                     /translation="MKVLNGWSDRKMWRVLSALPIGVVFFDLIYGFVLNVLQGLDLQR
                     AVPDSEGVLAVTPDIAFNSLQIVANGGMAAVVCFGLAVVFLLNRSVRRRQVLEIGVFR
                     MLGLVAVLAFSAPSLWEWANALPLLLKGADVVNTGNARYVLTALCMPFPAVSCIIGLV
                     GRFRLQTASGRVAKAGGAVKAGG"
     gene            complement(686..790)
                     /locus_tag="WNA25_000859"
                     /pseudo
     CDS             complement(686..790)
                     /locus_tag="WNA25_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(840..1511)
                     /locus_tag="WNA25_000860"
                     /pseudo
     CDS             complement(840..1511)
                     /locus_tag="WNA25_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557955.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            1578..1880
                     /locus_tag="WNA25_000861"
     CDS             1578..1880
                     /locus_tag="WNA25_000861"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823650.1"
                     /translation="MKDFDEVCEKWEKSFKENGKEIEYNQKYSRIYGEVRGAGFNGDI
                     GEKLHRCAVMEWAENETVKLAQKWEQEQKKQQIQQKKETEKSPKHKASRDDWEMER"
     gene            complement(1962..4049)
                     /locus_tag="WNA25_000862"
     CDS             complement(1962..4049)
                     /locus_tag="WNA25_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HFA9823651.1"
                     /translation="MDYLSRLSFGFNKKLPVILQTEVAECGLACLTSILSYYGFHTDL
                     RTLRQKYTLSLKGANLADIMRFGNEMNLTPRALRLELDELSNLQLPCILHWNLNHFVV
                     LCSISKDSIVIMDPAVGMRKIKMDEVSQKFTGIALELFPNTHFEEKKETKKIKILSLL
                     RGISGLKRSLIQMLILAISLEVFALVSPFFMQWVIDHIIVTADKNLLLTLTLGFGLLA
                     ILQQLISLLQAWVGMHLSTTLNLQWKANIFKRLLDLPNDYFSKRHLGDVISRFGSIDH
                     IQETLTSTFFVLVLNSLMAIFTFVLMTIYSTQLSLIVLLTLVLYILIRWLAYYPLRNA
                     TEENIVHEAKQNSYFMETIRGIQSVKLFDKHYQRHGTWMSLFVNTVNTKLTTDKLSAL
                     FEFSNKLLFRMENIIIIYLGASAILDGSFTVGVLMAFLAYKGQFESRTASLVDQYIQI
                     KMLGLHAERLADITLNETETEIIKYNHIPKLDNEQLVLKVENVSFRYADNEPYLFENI
                     NLEFKDNEAVVLTGQSGRGKSTLLNILTGSLKPETGTVSINGHDIYQVSPSFIRGLSG
                     IVRQDDVLFAGSIGENISFFDESPNMELIEQCAKMAQIHDDILKMPMGYETLIGDMGN
                     ILSGGQKQRVILARALYKRPKILFLDEASSHLDVENEQKINHNLKSLGIMKIMVAHRQ
                     ETIQSADKILNLG"
     gene            complement(4052..5317)
                     /locus_tag="WNA25_000863"
     CDS             complement(4052..5317)
                     /locus_tag="WNA25_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HFA9823652.1"
                     /translation="MSHLFRKEVFVAQQNKWTGQVILTRPFSFLFLTFCAFLIALCII
                     IFLIFGSYTNKTTVEGQLLPTMGVVRVYSSDIGTITHKFVEDGNFVKAGEPLFKLSTS
                     RFGEKGNVQAKLAAEANLKKTLALQELERLKRIHQNEQKNVHNNIHRLNNQLENIKQQ
                     ITGQNRQIRLAEKTLNKNKFLASQGAVSQQDKMTAESHLLEQRSRLESLKREQNNAIR
                     ELDEQKITLSSLPERHKTELSQLNRAITEMNQEILDFDLKSEQTIRASKSGYISTINV
                     DIGQQVEPSKLLLSIVPEQTELVANLYIPSKAVGFIKPKDKVVLRYQAYPYQKFGHAT
                     GEIISVARTALGKQELSGLGIIFTNPTLLNEPAYLVKVKLEKQTIKAYGENKPLQIGM
                     ILEADILHERKKLYEWVLDPLYSISGKIN"
     gene            complement(6038..6319)
                     /locus_tag="WNA25_000864"
     CDS             complement(6038..6319)
                     /locus_tag="WNA25_000864"
                     /inference="COORDINATES: protein motif:HMM:NF021923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HFA9823653.1"
                     /translation="MSNDENLLFSQPHLTKVNIMQVLTLDEIQQVSGAACNWRDFSKN
                     TIGSALGGAAGGAIVGSFAGGIGAIPGAKFGAIGGAITGSVQYGSTCWW"
     gene            complement(6540..6776)
                     /locus_tag="WNA25_000865"
     CDS             complement(6540..6776)
                     /locus_tag="WNA25_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin transporter"
                     /protein_id="HFA9823654.1"
                     /translation="MKELHTSELVEVSGGKFHISAQGGGNLGKKDMVAVGKIGVSYSP
                     NNSGVEFSVSKQFGYVQGLGVQFSKPTFGFSKKW"
     gene            complement(7732..8058)
                     /locus_tag="WNA25_000866"
                     /pseudo
     CDS             complement(7732..8058)
                     /locus_tag="WNA25_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            8318..9790
                     /gene="pyk"
                     /locus_tag="WNA25_000867"
     CDS             8318..9790
                     /gene="pyk"
                     /locus_tag="WNA25_000867"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFA9823655.1"
                     /translation="MNQTSRDLTRISHNTKIVATLGPGSNNVELLEDMIRVGGLNVVR
                     FNFSHGTPEFHQENARIVREAAKRAGQEIAIIADLQGPKIRVGKIAGGGIELNKGETL
                     VLDAALEGEGTREAVGLDYRDLPDDVAAGDVLWLDDGLLTLTVESVEGSRIITRVENS
                     HILKSNKGINKRGGGLSAGALTEKDFRDLKTAIAIGCDYLAISFVKSAEDLHIARAKV
                     EEEMKGSTAVRPGLVSKIERVEAIENLDEIILAGDGIMVARGDLAVEVGHAAVPALQK
                     RMIRRARELRRFSITATQMMESMITNPVPTRAEVSDVANAVLDGTDAVMCSAETAVGA
                     YPFETVSQMAIICAAAEKEQDSLNGVAEQTEYPEAVSTNLAIAGGAVSVARAVHAKAI
                     VALTESGSTAFEISRHNITLPIFALTPSVSAQRRMAMYRGVRPLILATSTDHDTALNE
                     VETMLVEHNILHSGDQYIITSGSQMRESGSTNTLEVLRVK"
     gene            9949..10548
                     /locus_tag="WNA25_000868"
     CDS             9949..10548
                     /locus_tag="WNA25_000868"
                     /inference="COORDINATES: protein motif:HMM:NF015316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HFA9823656.1"
                     /translation="MPFLFVHRPPFYGNRNDPFHTEKTVLLLGTAFAAASVHASGYHF
                     GTQSVNAQSTANAADASTIFYNPAGLTKLDSSQISVNANIVLPSIHYEADSATDFTGL
                     PVQGSKNGKITKTTVAPHIYGAYKVNDNLTVGLGVYVPFGSATEYEKDSVLRHNINKL
                     GLTSIAVEPVAAWKLNERHSFGAGIIAQHNSAELRKYAD"
     gene            10570..11376
                     /locus_tag="WNA25_000869"
     CDS             10570..11376
                     /locus_tag="WNA25_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HFA9823657.1"
                     /translation="MLQATPSNPTAAAQIKADGHADVKGSDWGVGYQLAWMWDINDRA
                     RVGVNYRSKVSHTLKGDAEWAADGAAAKQQWNDNMLTPLGYTANEKARVKIVTPESLS
                     VHGMYKVSDKADLFGDVTWTRHSRFNKAELFFEKEKNIANGKKSDRTTITPNWRNTYK
                     VGLGGSYQISEPLQLRVGIAFDKPPVRNADYRMNSLPDGNRIWFSAGMKYHIGKNHVV
                     DAAYTHIHINDTSYRTAKASGNDVDSKGASCARFKNHADIIDLQYTYKFK"
     gene            complement(11459..11752)
                     /locus_tag="WNA25_000870"
     CDS             complement(11459..11752)
                     /locus_tag="WNA25_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823658.1"
                     /translation="MKANDKLNRQIDVLQKQSAAIHNEAYIEMNTLLYRHREVVSIHN
                     RKADYAEKGKERIALFPRGLNGITKLPAAVLLPERPYHFDMKEVLYIFSRIPR"
     gene            complement(12064..12213)
                     /locus_tag="WNA25_000871"
     CDS             complement(12064..12213)
                     /locus_tag="WNA25_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823659.1"
                     /translation="MPSETLLSVSDGILTNTVRRRKTAGFSCVSYADVSTHRTAHKAS
                     PYVPS"
     gene            complement(12231..12428)
                     /locus_tag="WNA25_000872"
     CDS             complement(12231..12428)
                     /locus_tag="WNA25_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823660.1"
                     /translation="MNTVPKSRIPVKPLPEKTTAEAKVEKWRQLGADHGLSGEWAVAA
                     RLGENGFTEEQMENIANLFGR"
     gene            complement(12438..13454)
                     /locus_tag="WNA25_000873"
     CDS             complement(12438..13454)
                     /locus_tag="WNA25_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HFA9823661.1"
                     /translation="MYRKLIALPFALLLAACGREEPPKALECANPAVLQDIRGSIQET
                     LTQEARSFAREDGRQFVDADKIIAAAYGLAFSLEHASETQEGGRTFCIADLNITVPSE
                     TLADAEANSPLLYGETSLADIVQQKTGGNVEFKDGVLTAAVRFLPAKDARTAFIDNTV
                     GMATQTLSAALLPYGVKSIVMIDGKAVTKEDAVRVLSGKAREEEPSKPTPEDILEHNA
                     AGGDAGVPQAAEGAPEPEILHPDDVERADTVTVSRGEVEEARVQNQRAESEITKLWGG
                     LDTDVQKELVGEQRKWAQEKISNCRQAAAQADRQEYAEYLKLQCDTRMTRERIQYLRG
                     YSID"
     gene            complement(13553..14767)
                     /gene="gltS"
                     /locus_tag="WNA25_000874"
     CDS             complement(13553..14767)
                     /gene="gltS"
                     /locus_tag="WNA25_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFA9823662.1"
                     /translation="MEWAFNSYYTLIAATLVLLVGKVLVKKIKILRDFNIPEPVAGGL
                     IAAIILFALHEAYGVSFKFEKPLQNAFMLIFFTSIGLSADFSRLKAGGLPLVVFTAIV
                     GGFILVQNFVGVGLATALGLDPLIGLITGSVSLTGGHGTSGAWGPNFETQYGLVGATG
                     LGIASATFGLVFGGLIGGPVARRLINKMGRKPVENTKQDQDDNADDVFEQAKRTRLIT
                     AESAVETLAMFAACLAFAEIMDGFDKEYLFDLPKFVWCLFGGVVIRNILTAAFKVNMF
                     DRAIDVFGNASLSLFLAMALLNLKLWELTGLAGSVTVILAVQTAVMVLYATFVTYVFM
                     GRDYDAAVLAAGHCGFGLGATPTAVANMQSVTHTFGASHKAFLIVPMVGAFFVDLINA
                     AILTGFVNFFKG"
     gene            complement(14875..14979)
                     /locus_tag="WNA25_000875"
                     /pseudo
     CDS             complement(14875..14979)
                     /locus_tag="WNA25_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(15020..15301)
                     /locus_tag="WNA25_000876"
     CDS             complement(15020..15301)
                     /locus_tag="WNA25_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823663.1"
                     /translation="MKQIKASDFTDEHFGLPTILDILSELEKPQPRTDSRPQTTTQRA
                     CPNQFGDGGCVCEVEAVSPTAGMYSDRCSGGFCPRFFCYFKYVYSHDAV"
     gene            complement(15320..16444)
                     /locus_tag="WNA25_000877"
     CDS             complement(15320..16444)
                     /locus_tag="WNA25_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFA9823664.1"
                     /translation="MSNNQPNSQIYEDYWAFTNAFTNYNDQKFCTALNICLDFIDANQ
                     DQPYSNELYEALQQNIAQDSVMASQRTSKAMNLASVRKAINQFVKMGFIEPFLSSYTP
                     LSRDYVQARTNRKRQTLLSKIVYTHSGFQRSVTENSNIRQINFLIKTLVEHPQGKLNK
                     KEIAAMMLVDLKTFQQDYLTETELNDYFQQGIESGFIERKYNQISYLWNLLDKLDDLK
                     RVGDDLYFAEDAQRIFGNLDEITVRKRDPYLHRLYKNQLQEESEEHYGNVKCMLEKLA
                     YPVLIASHIKPFILSDDTEAYDPNNGLLLSRTLDSLFDLKYISFDDEGNMVKSKRLSD
                     DVWRRWCDVKLDNNLLNDKRKSYLAYHRELMLQEDQEFHI"
     gene            complement(16507..17514)
                     /locus_tag="WNA25_000878"
     CDS             complement(16507..17514)
                     /locus_tag="WNA25_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HFA9823665.1"
                     /translation="MNKKYPKINYIGNKEKIASWICDQLPSDVDTVADVFSGGCSFAY
                     EAKKRGYRVITNDILAINYQIALALIENNHETLNDDDVAMIFSGSPHAGFMSQRYSEK
                     FYFHDECQQLDLYRKNIGKLNNQYKRALAFTLMRRAMIRKMPYSRFTIPWEKVKQLRD
                     EEYSYAKYGRRRAYHNESFQSHFLQNLDDYNQAVFNNGWRHHAFNQDIFDLLPNIQAD
                     AVYLDPPYTGTMNNYFGFYGLLDSYITSSIPKPFANHFMDKKQAVELFKRVINHLKPF
                     KYWLLSYNNASYPNRDELEEMLGKNGRNVQILETPHVYKVTGKEKKQSHKEILFLVEN
                     T"
     gene            complement(17507..18508)
                     /gene="dcm"
                     /locus_tag="WNA25_000879"
     CDS             complement(17507..18508)
                     /gene="dcm"
                     /locus_tag="WNA25_000879"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HFA9823666.1"
                     /translation="MHTPLTYIDLFSGAGGLSLGFEQAGFQQLLSVEMESDYCQTYRT
                     NFPRHQLLQKDLTTLTEQDLTNCLNGQSVDLVIGGPPCQGFSMAGKIGRTFTDDPRNH
                     LFKEFVRIVKIVQPYFFVMENVARLYTHNSGKTRIEIIQAFQNIGYSVECKILSAADF
                     GVPQIRSRVIFIGRRDKGKISFPEPLQISHQTVGSAIGHFPKLAAGESNPHVANHEAM
                     NHSAQMLEKMAFVKNGGNRNDIPEPLRPKTGDIRKYIRYNSNKPAVCITGDMRKVFHY
                     EQNRALTVRELAALQSFPDNFIFCGSKIAQQQQVGNAVPPLLAKAIAESILKMSENE"
     gene            complement(18539..20812)
                     /locus_tag="WNA25_000880"
     CDS             complement(18539..20812)
                     /locus_tag="WNA25_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HFA9823667.1"
                     /translation="MNITRILSQELSATTVQINAAIELLDDGATVPFIARYRKEATGG
                     LDDTQLRQLAERLQYLRELEERKAVVLKSIEEQGKLSDDLRAQIEAADNKTALEDLYL
                     PYKPKRRTKAQIAREHGLQPLADVLLAEQPQDVEAAAQGYLNENIPDAKAALDGARAI
                     LMEQFAEDAELIGTLRDKLWNEAEIHTQVVEGKETEGEKFSDYFDRREPVRAMPSHRA
                     LAVLRGRNEGVLNIALKYQPDDTPITQQSEYEQIIARRFKVSDGHKWLRDTVRLTWRA
                     KIFLSLELEALNRLKEAADTDAITVFARNLKDLLLAAPAGRLTTLGLDPGYRNGVKCA
                     VVDDTGKLLDTVIVYLHQENNMLATLSRLIKQHGVKLIAIGNGTASRETDKIAGELVR
                     GMPESSLHKIVVSEAGASIYSASELAAREFPDLDVSLRGAVSIARRLQDPLAELVKID
                     PKSIGVGQYQHDVNQSRLAKSLDAVVEDCVNAVGVDANTASAPLLARISGLNQTLAQN
                     IVAYRDENGAFDSRKKLLKVPRLGEKTFEQAAGFLRINGGKEPLDASAVHPEAYPVVA
                     KMLAQQGITAAELIGNRERVKQIKASDFTDERFGLPTILDILSELEKPGRDPRGAFQT
                     ASFAEGIHEISDLQVGMILEGVVSNVANFGAFVDIGVHQDGLVHISALSNKFVQDPRD
                     VVKAGDVVKVKVLEVDAARKRIALTMRLDDEPGGAKHKMPSENRSRERTAGRKPQRND
                     RAPTNSAMADAFAKLKR"
     gene            20927..21943
                     /gene="galE"
                     /locus_tag="WNA25_000881"
     CDS             20927..21943
                     /gene="galE"
                     /locus_tag="WNA25_000881"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFA9823668.1"
                     /translation="MTVLITGGTGFIGSHTAVSLVQSGYDAVILDNLCNSSAAVLPRL
                     RQITGRNIPFYQGDIRDCQILRQIFSEHEIESVIHFAGLKAVGESVAEPTKYYGNNVY
                     GSLVLAEEMARAGVLKIVFSSSATVYGDAEKVPYTEDMRPGDTANPYGASKAMVERML
                     TDIQKADPRWSVILLRYFNPIGAHESGLIGEQPNGVPNNLLPYICQVASGRLPQLSVF
                     GGDYPTPDGTGMRDYIHVMDLAEGHIAAMKAKGGVAGVHLFNLGSGRAYSVLEIIRAF
                     EAASGLHIPYRIQPRRAGDLACSYADPSHTKQQTGWETKRGLQQMMEDSWRWVSRNPG
                     RYGD"
     gene            21944..22984
                     /gene="rfbB"
                     /locus_tag="WNA25_000882"
     CDS             21944..22984
                     /gene="rfbB"
                     /locus_tag="WNA25_000882"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFA9823669.1"
                     /translation="MQTAGKKNILVTGGAGFIGSAVVRHIIQNTRDSVVNLDKLTYAG
                     NLESLTDIADNPRYAFEQVDICDRAELDRVFAQYRPDAVMHLAAESHVDRAIGSAGEF
                     IRTNIVGTFDLLEAARAYWQQMPSEKREAFRFHHISTDEVYGDLHGTDDLFTETTPYA
                     PSSPYSASKAAADHLVRAWQRTYRLPSIVSNCSNNYGPRQFPEKLIPLMILNALSGKP
                     LPVYGDGAQIRDWLFVEDHARALYQVVTEGVVGETYNIGGHNEKTNLEVIKTICALLE
                     ELAPEKPAGVARYEDLITFVQDRPGHDARYAVDAAKIRRDLGWLPLETFESGLRKTVQ
                     WYLDNKTRRQNA"
     gene            23089..23955
                     /gene="rfbA"
                     /locus_tag="WNA25_000883"
     CDS             23089..23955
                     /gene="rfbA"
                     /locus_tag="WNA25_000883"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFA9823670.1"
                     /translation="MKGIILAGGSGTRLYPITRGVSKQLLPVYDKPMIYYPLSVLMLA
                     GIRDILVITAPEDNAAFQRLLGDGSDFGIRLQYAVQPSPDGLAQAFIIGEEFIGNGNV
                     CLILGDNIFYGQSFTQTLKQAAAKTHGATVFGYRVKDPERFGVVEFDENFNALSIEEK
                     PQQPKSDWAVTGLYFHDNRAVEFAKQLKPSARGELEISDLNRMYLEDGSLSVQILGRG
                     FAWLDTGTQESLHEAASFVQTVQNIQNLHIACLEEIAWRNGWLTKKDVETRAKPLEKT
                     AYGQYLLRLIGK"
     gene            24094..24651
                     /gene="rfbC"
                     /locus_tag="WNA25_000884"
     CDS             24094..24651
                     /gene="rfbC"
                     /locus_tag="WNA25_000884"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFA9823671.1"
                     /translation="MDIIDTALPDVKLLKPQVFTDGRGFFMETFRDGWFKENIADRTF
                     VQENHSNSSKGVLRGLHYQTENTQGKLVRVVVGEVFDVAVDMREGSPTFGKWAGATLS
                     AQNRYQLWIPEGFAHGFCVLGDAAEVVYKCTDYYNPETEQVLIWNDPAIGIGWPLQTA
                     PLLSPKDLAGKTWAQAEKLRLTLSR"
     gene            complement(24695..26713)
                     /locus_tag="WNA25_000885"
     CDS             complement(24695..26713)
                     /locus_tag="WNA25_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HFA9823672.1"
                     /translation="MNKSLSGSVEEYRELTLRGMILGALITVIFTASNVYLGLKVGLT
                     FASSIPAAVISMAVLKFFKGSNILENNMVQTQASAAGTLSTIIFVLPGLLMAGYWSGF
                     PFWQTTLLCIAGGILGVIFTIPLRYAMVVKSDLPYPEGVAAAEILKVGGHEEGDDRQG
                     GSGIKELAAGGALAGLMSFCAGGLRVIADSASYWFKSGTAIFQLPMGFSLALLGAGYL
                     VGLTGGIAILLGISIAWGIAVPYFSSHIPQPSDMEMAAFAMKLWKEKVRFIGAGTIGI
                     AAVWTLLMLLKPMVEGMKMSFKSFGGGAPATERAEQDLSPKAMIFWVLSMMFILGVSF
                     YHFIGDSHITGGMAWLLVAVCTLLASVIGFLVAAACGYMAGLVGSSSSPISGVGIVSI
                     VVISLVLLLVGESGGLLADEANRKFLLALTLFCGSAVICVASISNDNLQDLKTGYLLK
                     ATPWRQQVALIIGCIVGALVISPVLELLYEAYGFTGAMPREGMDAAQALAAPQATLMT
                     TIASGIFAHNLEWAYIFTGIAIGAVLIVVDLVLKKSSGGKLALPVLAVGMGIYLPPSV
                     NMPIVAGAVLAAVLKHIIGKKAENREGRLKNADRIGTLFAAGLIVGESLIGVIMAFII
                     AFSVTNGGSDAPFALNLQNWDAAASWLGLAFFVTGMFFFAQRVLKAGR"
     gene            complement(26908..28029)
                     /gene="dnaJ"
                     /locus_tag="WNA25_000886"
     CDS             complement(26908..28029)
                     /gene="dnaJ"
                     /locus_tag="WNA25_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFA9823673.1"
                     /translation="MSNQDFYATLGVARAATDDEIKKAYRKLAMKYHPDRNPDNKEAE
                     EKFKEVQKAYETLSDKEKRAMYDQYGHAAFEGGGQGGFGGFGGFGGAQGFDFGDIFSQ
                     MFGGGSGRAQPDYQGEDVQVGIEITLEEAAKGVKKRINIPTYEACDVCNGSGAKPGAS
                     PETCPTCKGSGTVHIQQAIFRMQQTCPTCRGAGKHIKEPCVKCRGVGRNKAVKTVEVN
                     IPAGIDDGQRIRLSGEGGPGMHGAPAGDLYVTVRIRAHKIFQRDGLDLHCELPISFAT
                     AALGGELEVPTLDGKVKLTVPKETQTGRRMRVKGKGVKSLRSSATGDLYCHIVVETPV
                     NLTDRQKELLEEFERISTGLENQTPRKKSFLDKLRDLFD"
     gene            28163..>28334
                     /locus_tag="WNA25_000887"
     CDS             28163..>28334
                     /locus_tag="WNA25_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFA9823674.1"
                     /translation="MSTFFRQTAQAMTAKHIGRFPLLELDQVIDWQPIEQYLNRQKTR
                     YLRDRRGRPAHPL"
BASE COUNT         7537 a         7214 c         6346 g         7237 t
ORIGIN      
        1 ggcaacgccg tactggtttt tgttaatcca ctataaaagc aaagaaaccg ccattagcac
       61 tacacccccc caagctgcgg ctgccaaaca catcggctgc ttctcgacgc acccgctgaa
      121 aaatgcgccc tatccgcccg ccttgaccgc accccctgcc tttgccaccc tgcccgatgc
      181 cgtctgaagc ctgaaccgcc ctaccagccc gatgatgcac gacaccgccg gaaagggcat
      241 acacaaagcc gtcagcacat aacgcgcatt ccccgtattg accacgtccg cgcccttcag
      301 cagcagcggc agcgcgttcg cccactccca cagcgaaggc gcgctgaacg ccaataccgc
      361 caccagcccc aacatccgga acaccccgat ttccaagacc tgccgccgcc gcaccgaacg
      421 gttgagcaaa aacacaaccg ccaacccgaa acagaccacc gccgccatac cgccgttggc
      481 gacaatctgc aaactgttga acgcaatatc gggcgtaacc gccaacacgc cttccgaatc
      541 cggcacggca cgctgcaaat ccaacccctg caacacattc aacacaaaac cgtagatcaa
      601 atcaaaaaac accacgccta tcggcaaagc actcaaaacc cgccacatct tcctatctga
      661 ccaaccgttc aaaaccttca ttttatagtg gattaaattt aaaccagtac ggcgttgcct
      721 cgccttgccg tactatttgt actgtctgcg gcttcgtcgc cttgtcctga tttaaattta
      781 atccactata cccgaccacg caagccgccg aacaaaaaat aaggggctgt cctagataac
      841 taggataaac tcgattttac taattgtttt aaaatggaac aagaactttt atctcactgt
      901 tgttaaaacg ccattcgcac tcctttaaat acagctcaaa atgcgctttg ggaatgccgt
      961 taaacttgcg taaatgacgt tttgcctgat tccaaaagtt ctcaattcca ttaatatggt
     1021 tttgtcgttc ggcaaaatgt gtgctgtgat tgatacgaaa acgaagtttc agcgaagcta
     1081 aaatggctaa attcgctcac atctaataca tcatagctac gataacaatc cgtataaaca
     1141 atactgtcag gtttcacttg ttcacggata ataggaaata aagtagcggt ttgagtattc
     1201 ggtactgtaa ccgtataaac cttaccattt cgcttcaaaa gaccgaatac ggcgacttta
     1261 ccggcagcac cgcgaccgcg tttgcctttg cgttgtccgc caaaataact ttcatctgct
     1321 tctacttcgc catcaaacat ttccaaatgc ggactgtttt gataaataag taatcgtaaa
     1381 cgatgaaaat aataggctgc ggtattttta ttaacgccta ctaactctgc tgccgttctt
     1441 gcagttacac ctgcgacaaa cagttcaatg agtttatttt gtttatactg gcttagacga
     1501 ctttttctca tagggataat tctaacttaa tttgaatttc cctagttatc taggacagcc
     1561 ccttaaaaaa tatccctttg aaagattttg atgaagtttg tgaaaagtgg gagaaatcct
     1621 ttaaagaaaa tggaaaagaa attgaatata atcaaaaata ttcaagaatt tatggtgaag
     1681 ttcgtggagc tggctttaat ggcgatatcg gagaaaagct acatcgttgt gccgtgatgg
     1741 aatgggctga aaatgagacc gtgaaattag cccaaaaatg ggaacaagag cagaaaaaac
     1801 aacaaattca acagaaaaag gaaactgaaa aatcgccaaa acacaaagcc agtcgtgatg
     1861 attgggaaat ggaacgttaa accgctattt tcatgtattc tattaaatta tttgaaaaaa
     1921 gacttcactt tttgttaaag aaaaatgaag tcttgttctg ttcaacctaa attcagaatt
     1981 ttatctgccg attgaattgt ttcttggcgg tgtgcaacca ttattttcat aataccaaga
     2041 ctttttaggt tatggttaat tttttgttca ttttctacat ctaaatggct acttgcttcg
     2101 tctaaaaata gaattttggg tcgtttatac aatgcacgag ccaagataac tctctgcttt
     2161 tgtccacctg ataagatatt tcccatatcg ccaatcaagg tctcatagcc cattggcatt
     2221 ttaagtatat cgtcatgtat ttgtgccatt tttgcacatt gttcaatgag ctccatattt
     2281 gggctttcat caaaaaatga aatattttcc ccaatagaac ctgcaaaaag gacatcatct
     2341 tggcgaacaa tcccgctcaa tcccctaata aaggatggag aaacttgata tatatcatgc
     2401 ccattaatac taactgtacc agtttcaggt tttaggctac ctgttaaaat gtttaacaaa
     2461 gtggacttcc cccgaccaga ttgtcctgtt aaaacaactg cttcattatc tttgaattcc
     2521 aaattaatgt tttcaaaaag atatggctca ttatcagcat atctgaatga gacgttttca
     2581 actttaagaa ccagttgttc attatctaat ttaggtatat gattatactt aataatttca
     2641 gtttctgttt catttaaagt aatgtcagcc aaacgttcag catgaagccc taacattttg
     2701 atttggatgt attggtcaac gagagaagct gttctgcttt caaattgccc tttataagcc
     2761 aaaaaagcca tcagaacacc gactgtaaat gaaccatcta aaattgcgct tgcaccaaga
     2821 taaattatga taatattttc cattctaaac aacagtttat ttgaaaattc aaataaagca
     2881 gagagtttat ctgttgtcag cttggtattg actgtattca caaataggct catccaagtg
     2941 ccatgtcttt gataatgttt atcaaataat ttaactgatt ggataccacg aatggtttcc
     3001 atgaaatatg agttttgttt ggcttcatga acaatatttt cttctgttgc atttcttaat
     3061 gggtaatatg caagccaacg aattagtatg tacaaaacaa gtgttaaaag aacaatcagc
     3121 gataattgag tgctgtaaat tgtcattaac acgaaagtaa aaatagccat taagctattt
     3181 aaaactaaaa caaaaaaagt agaagttagt gtttcttgga tatgatctat tgaaccaaat
     3241 cttgaaatca catctcctaa atgtcgttta ctgaaatagt cattaggtaa gtcaagtaac
     3301 cttttaaata tattggcttt ccattgtaaa ttaagagttg tagataggtg catacctacc
     3361 catgcttgta acaggctaat taactgttgc aggatagcca gtaaaccaaa tcccaaagta
     3421 agggtcagta ataaattttt atcagcagtt acaatgatat ggtctattac ccattgcata
     3481 aagaatggac taaccaatgc aaagacttcc aaagaaatag ctaatataag catttgaatt
     3541 aaagagcgtt ttaagcctga aatccccctt aatagagata atattttgat tttctttgtt
     3601 tcttttttct cttcaaaatg ggtattgggg aataattcta gggcaatccc tgtgaatttt
     3661 tgtgaaactt cgtccatttt gatttttcgc ataccgacag cagggtccat aatgacgata
     3721 ctgtctttgg aaatggaaca aagtacaaca aaatggttta agttccaatg gagaatgcag
     3781 ggtagttgta aatttgacag ctcatctaac tctaaacgca aagctcgtgg cgttaaattc
     3841 atttcattgc caaatctcat gatgtctgca agatttgcgc cctttaatga cagggtgtat
     3901 ttttggcgta acgttcttaa atcagtgtga aagccataat aggacaagat ggatgtcagg
     3961 catgctaaac cacattcagc aacttctgtt tgcagaatga caggtagctt tttgttaaat
     4021 ccaaaggaca gtcttgataa ataatccatt tttaattgat ttttcctgaa atgctgtaaa
     4081 gtgggtcaag tacccattcg tacaattttt ttcgttcatg gagaatatct gcttctaaaa
     4141 tcatgccaat ttgaagcggc ttgttttctc cgtatgcttt aatcgtttgt ttttccaatt
     4201 taactttcac aagataggca ggttcattta ataaggttgg gttagtgaaa atgataccta
     4261 aacctgatag ctcttgttta ccgagagcag ttctggcaac tgaaataatt tctcctgtgg
     4321 catgtccaaa tttttggtaa gggtacgctt ggtaacgtaa aacaacttta tctttcggtt
     4381 taataaaacc aacagcttta ctgggtatgt aaagattggc gaccaattca gtttgttcag
     4441 ggacaatgct taacagcaat ttagacggtt caacttgttg ccctatatca acattaattg
     4501 ttgaaatata acctgattta ctagctcgta tggtttgttc ggatttcaaa tcaaaatcca
     4561 aaatttcttg gttcatttcc gtaatcgcac ggttgagttg gctcaattcg gttttatggc
     4621 gttcaggcag gctgcttaat gtgattttct gttcatcaag ttccctgatt gcattatttt
     4681 gttcacgttt taggctctcc aaacgtgagc gttgttccaa taaatggctt tcggcggtca
     4741 tcttatcttg ttgggatact gcgccttgac tggctaaaaa cttgttcttg ttaagggttt
     4801 tttccgctaa acgaatttga cgattttgcc ctgtaatttg ctgtttaata ttctctaatt
     4861 gattgtttaa acgatgaatg ttgttatgaa catttttttg ctcattttga tgaatgcgct
     4921 ttaaacgttc caattcttgt aatgccaaag tttttttaag gttggcttct gctgccaatt
     4981 tggcttgtac gtttcctttt tcgccaaaac gcgatgtgga aagtttgaac aatggttcgc
     5041 cagctttgac aaagttacca tcttcaacaa atttatgcgt aatcgtgccg atatcggaag
     5101 agtaaacacg aaccaccccc atagttggaa gtaattgacc ttcaacggtt gttttattgg
     5161 tatagctacc aaaaatcaaa aagataatga tacacagagc aatgagaaaa gcgcaaaaag
     5221 tcagaaataa aaaagagaat ggacgggtca agataacctg acctgtccac ttattttgtt
     5281 gggctacaaa gacttctttt cggaataagt gggacattta ttattatttc ttaaaaaaaa
     5341 tggatagaaa aacaccaata attaaatact tatgcctaat aacataaatt tgctattgat
     5401 tgatgtttct attttaatta cattaaattt aaccaataat ccagaaatgg ttgcagctaa
     5461 aatgcaaaag acagtggcgt aaatggtttc agtattcaca atttttctct ttcaaaattg
     5521 ttcaatctaa atagaaaaag gtaacttcaa tacatattgc tgtaactgaa attacctttt
     5581 ccttaatgat gtaattatct tatggcttca tcatgccacg accgatattg tatccgccag
     5641 caacagagaa aataccagct cccattgcgt caacccagcc tactggtcct gtccacttgt
     5701 tccgttgagc tacaaagact tctggtctga aaaatgagtg attgttagac atgtgtactt
     5761 atttatgttt ctttgattgg gataaatttg taaaaacaaa aaaaaggcaa gttgttatta
     5821 tagaagaact taaaatgctt aacatactca ttgaggtttg taccacataa tgagatacga
     5881 gataatttgt tgggagtaaa atcaaaaaaa agattaaata tttaaaataa tcactatatt
     5941 ttttcattat ttgttccttt ttgtatttaa gttcttatga aaaactaggg tattttgaat
     6001 agaaaatatc aaaataccct agttttatta aggaatatta ccaccaacaa gtgcttccat
     6061 attgtacaga accagtgatt gcaccaccaa tagctccgaa tttcgcacct ggaatagcac
     6121 caataccacc tgcaaatgaa ccaacaattg ccccaccagc tgctccacct aatgcactac
     6181 caatggtatt ttttgagaaa tcacgccagt tacaagcagc accagaaact tgttgaattt
     6241 catccaaagt caaaacttgc ataatattca cctttgttaa gtgtggttga gaaaaaagta
     6301 aattttcatc gttactcatt atttgcaatc atgcatatac tgtaaccaag cactatattc
     6361 agttggatca tagtttttac tactgggttg tctgatatga ctattggcac tattggcaca
     6421 actgggatta gcaactttat tgtgagtacc taatggattg atatttcctg taaagtggct
     6481 attgcatgcg cttaatgagc atagatttat taatgtcaga aaggataaaa caaaaaatct
     6541 taccattttt tactaaaacc aaaagtaggt ttcgaaaact gtacaccaag accttgtaca
     6601 tatccaaatt gcttgctaac agaaaactct actccactat tgttagggga ataggaaaca
     6661 ccaattttac caacagcaac catatctttt ttacctaggt tgccgccacc ctgtgcagag
     6721 atatggaatt tgccacctga cacttcaact aattcagagg tgtgtaactc tttcatgatt
     6781 ttctcctgta aagaagtatg gttggaaatg taaatcagaa tcaatctttc ttaagtttaa
     6841 tttaatactt ttttttgcaa gtattttatt ttaaattaaa tcaatatttt aaaaatattt
     6901 acttgtatac tgaaaaagaa actcctaatg gcggagtggg gcttttgttt aagtaagcat
     6961 tgaaaaaatt taaatttccc attatgatta acttaatgca agagcttttt caggacgtac
     7021 aaaccagcga atgatgtggg caggttggtt gtcgctaacc aatttcagta gctcaatcgt
     7081 cagattggta tccgacccta tcaagctaag gagaggctgt ggctactaca caaagcacct
     7141 gaaattattg ggtgcttttg tctttttatg gcattgtacc aatttgtgta ccacgagggc
     7201 ttgttcgccc aaacgcggta acaactgaat acacactttc cccctcaact tctttgcgtt
     7261 ctaaaacgac tgtcccaatc cgagaataga caatcatagg acgatgattg ccccttacat
     7321 cactaaattc actaaaaatt tttgcgcctg attgaaaaat catttccaca taaaagggtt
     7381 acacctgttt catcgtggct taatccaaac cgcgttaaat ctgctttgtg ttctgccaaa
     7441 atgtgaatca caccaaagcc gtgattaacc tttttgtgtt cgccgtgttt taaaacaatt
     7501 tgacctgccg ggaagtctgc tctattgttc ggacaataag gggaatatac caattacttt
     7561 aactacatca ttttttgaca tttttcttgc ctttcaattt aaaatccaaa atcatactgc
     7621 cataatttag caatccaaaa aaatttaggc agcagtaatg gtttcgtatt ttagaaaacg
     7681 aaacttggtt tggtgttttg aagcatttca gtacacaaac atggaatttt aagcggtttg
     7741 agtattcggt actgtaaccg tataaacctt accatttcgc ttcaaaagac cgaatacggc
     7801 gactttaccg gcagcaccgc gaccgcgttt gcctttgcgt tgtccgccaa aataactttc
     7861 atctgcttct acttcgccat caaacatttc caaatgcgga ctgttttgat aaataagtaa
     7921 tcgtaaacga tgaaaataat aggctgcggt atttttatta acgcctacta actctgctgc
     7981 cgttcttgca gttacacctg cgacaaacag ttcaatgagt ttattttgtt tataccggct
     8041 tagacgactt tttctcatag ggataattct aacttaattt gaatttccct agttatctag
     8101 gacagcccca aaataaattt agcctaaatc ataaaaccgt gtaattttgc accaaaaaag
     8161 gcggtttgca ggtaaaatat caaaccatta atttaaatca tatttttaca gaatattccg
     8221 ccgcgttcac caaatggaca tcaaaccggt tccaaatttc tgtaattttg caacaaaata
     8281 ccgcaaaaca cccgattgag accgaaagga ctttcatatg aaccagacaa gccgcgatct
     8341 gacccgcatc agccacaaca ccaaaatcgt cgccaccctt gggccgggca gcaacaacgt
     8401 cgaactgttg gaagacatga tccgcgtcgg cggcctgaac gtcgtccgct tcaacttcag
     8461 ccacggcacg cccgaattcc atcaggaaaa cgcccgcatc gtgcgcgagg cggcaaaacg
     8521 cgccggacag gaaatcgcca tcattgccga tctgcagggc ccgaaaatcc gcgtgggcaa
     8581 aatcgccggc ggcggcatcg aattgaacaa aggcgaaaca ttggtactcg atgccgcgct
     8641 cgaaggcgaa ggcacgcgcg aggcggtcgg tttggactac cgcgacctgc ccgacgacgt
     8701 tgccgcaggc gatgtcttgt ggctggacga cggcctgctg accctgaccg tggaatccgt
     8761 cgaaggcagc aggattatca caagggtgga aaacagccac atcctgaaaa gcaacaaagg
     8821 catcaacaaa cgcggcggcg gtttgtccgc aggcgcgttg accgaaaaag acttccgcga
     8881 cctgaaaacc gcgattgcca tcggttgcga ctatctcgcc atcagctttg tgaaatccgc
     8941 cgaagacctg cacatcgcgc gcgccaaagt cgaagaggaa atgaagggca gcactgccgt
     9001 ccgccccggt ttggtttcca aaatcgaacg cgtagaagcg attgaaaacc ttgatgaaat
     9061 catccttgcc ggcgacggca ttatggttgc gcgcggcgac ttggcggtcg aagtcggaca
     9121 cgccgccgtc cccgccctgc aaaaacggat gatccgccgc gcccgcgagt tgcgccgctt
     9181 cagcattacg gcgacgcaaa tgatggaatc gatgattacc aaccccgtgc cgacccgcgc
     9241 ggaagtcagc gatgtggcaa acgcggtatt ggacggcacc gatgcggtga tgtgttccgc
     9301 cgaaaccgcc gtcggcgcgt atccgtttga aaccgtcagc caaatggcga ttatctgcgc
     9361 ggctgcggaa aaagaacagg attcgctcaa cggcgttgcc gaacagacag agtatcccga
     9421 agccgtcagc accaacttag cgattgccgg cggcgcggtc agcgtggcgc gcgcggttca
     9481 cgccaaagcc atcgtcgccc tgaccgaaag cggttcgacc gcctttgaaa tcagccgcca
     9541 caatattacc ctgccgattt tcgcgctgac cccgagcgtt tccgcccaac gccgtatggc
     9601 gatgtaccgg ggcgtgcgcc cgctgatttt ggcaaccagc accgaccacg acacggcgtt
     9661 gaacgaagtc gaaacgatgc tggtggaaca caacatcctg cattccggcg accagtacat
     9721 catcaccagc ggctcgcaaa tgcgcgaatc cggttcgacc aatacgctgg aagtgctgcg
     9781 cgtcaaataa tccgccctga gtggaaaatg ccgtctaaag ccgatactgt cgggcttcag
     9841 acggcatttt atttgcattc cgtcaggtaa aaccgaaaga attgtgcaaa tccggcaaca
     9901 tcggataaaa tcgagtgcct atactaaagc gaaacaaggc atttccgact gcctttttta
     9961 tttgtccacc gtccgccttt ttacggaaac cgaaatgacc cctttcacac tgaaaaaacc
    10021 gtcctgctgc tcggcactgc ctttgccgcc gcatctgtcc acgcatccgg ctaccacttc
    10081 ggcacacagt cggtcaacgc gcaaagcacg gcaaatgccg ccgacgcgtc gaccatcttc
    10141 tacaatcccg ccggcctgac caaactcgac agcagccaga tttccgtcaa cgccaacatc
    10201 gtgctgccca gcattcatta tgaagcagat tccgccaccg actttaccgg gcttcccgtc
    10261 caaggttcta aaaacggcaa aatcaccaaa accacggtcg caccccacat ttacggcgca
    10321 tacaaagtca acgacaatct gaccgtgggc ttgggcgtgt acgtcccctt cggctctgcc
    10381 accgaatacg aaaaagattc cgtgttgcgc cacaacatca acaaactcgg tctgaccagc
    10441 atcgccgtcg aacctgtcgc cgcgtggaaa ctcaacgaac gccattcctt cggcgcaggc
    10501 atcatcgccc aacataattc cgccgaactg cgcaaatatg ccgactgagg aatcccaaaa
    10561 aaagcgcaaa tgctgcaagc aacaccttct aatcctactg ccgctgctca aatcaaggcc
    10621 gacggacacg ccgatgtcaa aggcagcgat tggggcgtcg gctaccaact ggcgtggatg
    10681 tgggacatca acgaccgcgc gcgcgtgggc gtgaactacc gttccaaagt ttcacacacg
    10741 ctcaaaggcg atgccgaatg ggcggcagac ggcgcggcgg cgaaacaaca gtggaatgac
    10801 aatatgctca caccgctcgg ttacacggcg aatgaaaaag cccgcgtcaa aatcgtaacg
    10861 cctgagtctt tgtccgtaca cggcatgtac aaagtgtccg acaaagccga cctgttcggc
    10921 gacgtaactt ggacgcgcca cagccgcttc aataaggcgg aactgttttt tgaaaaagaa
    10981 aaaaatattg ctaatggcaa aaaatccgac cgcaccacca tcacccccaa ctggcgcaac
    11041 acctacaaag tcggcttggg cggttcttat caaatcagcg aaccgctgca actgcgcgtc
    11101 ggcatcgctt ttgacaaacc gcctgtccgc aacgccgact accgcatgaa cagcctgccc
    11161 gacggcaacc gcatctggtt ctccgccggc atgaaatacc atatcggcaa aaaccacgtc
    11221 gtcgatgccg cctacaccca catccacatc aacgacacca gctaccgcac ggcgaaggca
    11281 agcggcaacg atgtggacag caaaggtgcg tcttgcgcac gtttcaaaaa ccacgccgac
    11341 atcatcgacc tgcaatacac ctacaaattc aaataaacgt tacaccgttt gaatataaaa
    11401 atgccgtctg aaacagattt ccgtttcaga cggcattttt atccggcagc cgatacgcct
    11461 atctcggtat tcgggagaat atatacagaa cctccttcat atcaaaatgg tagggacgct
    11521 cgggcaacag gacggcggca ggcagcttgg tgatgccgtt caaaccgcgc gggaacagcg
    11581 caatccgctc tttgcccttt tccgcataat ctgctttgcg gttgtggatg gaaactactt
    11641 cgcgatggcg gtaaagcagc gtgttcattt cgatataggc ttcgttgtgg atggctgccg
    11701 actgtttctg caacacgtca atctgcctgt tgagcttgtc gttggctttc atcagcctgt
    11761 tgaagaacga cggttgctcc tgcagtttgg cggtacaggc ttcgatactt tccgataccg
    11821 gcccgaactg ttcgaggtat ttttccagac agttttccaa gtcggtcagg cagccggcca
    11881 tcttgcggga aaattcttct tcaggctttc cctgccgaat ggacaatata gcggattaac
    11941 aaaaatcagg acaaggcgac aaagccgcag acagtacaaa tagtacggca aggcaaggcg
    12001 aggcaacgcc gtaccggttt ttgttaatcc actatatttg ggaggggcgt aaccccttcc
    12061 gaatcaggat ggcacatagg gcgacgcttt atgtgccgtc ctgtgtgttg aaacatcagc
    12121 ataggaaaca caggaaaagc cggctgtttt gcgcctgcgt accgtattgg tcaaaatgcc
    12181 gtctgaaacc gacaacaggg tttcagacgg catttccgtc aattttccct ttatctgccg
    12241 aacaggttgg cgatattttc catctgttct tcggtaaaac cgttttcgcc caatctggcg
    12301 gcaactgccc attcgcccga caaaccgtgg tccgcaccga gctgccgcca tttttcgact
    12361 ttggcttcgg ctgtggtttt ttcgggcagc ggtttgacgg gaatcctgct tttggggacg
    12421 gtattcatcg gtttgcccta atcgatggaa tagccgcgaa gatactgtat ccgttcgcgc
    12481 gtcatccgcg tgtcgcattg gagcttgagg tattcggcgt attcctgccg gtctgcctgc
    12541 gcggcggctt gtcggcagtt gctgattttt tcctgcgccc acttgcgctg ttcgccgacc
    12601 aactcttttt gcacgtcggt atcgagtcct ccccaaagtt tggtaatttc ggattccgca
    12661 cgttggtttt gtacgcgcgc ctcttccact tcgccccgtg atacggtaac ggtatcggca
    12721 cgctcgacgt cgtcgggatg caggatttcg ggttcgggcg cgccttctgc ggcttggggt
    12781 acgcccgcat cgccgccggc ggcattgtgt tccaaaatgt cttcgggggt gggtttggac
    12841 ggttcttctt cacgggcttt gccgctcaaa accctgaccg cgtcttcttt tgtcaccgcc
    12901 ttgccgtcta tcatcacgat gctcttcacg ccgtaaggca gcaacgcggc agacagcgtt
    12961 tgcgtcgcca taccgaccgt gttgtcgata aatgccgtcc gagcgtcttt ggcgggcagg
    13021 aagcggacgg ctgccgtcaa tacgccgtct ttaaactcga cattgccgcc cgtcttctgc
    13081 tgcacgatgt ctgccaaaga cgtttcccca tacagcaggg ggctgtttgc ctcggcatcg
    13141 gcaagcgttt cagacggcac ggtaatgttc aaatcggcga tacagaacgt gcgcccgcct
    13201 tcctgcgttt ccgaagcgtg ttccaaagaa aacgccaaac cgtaggcggc ggcgataatt
    13261 ttgtcggcat cgacaaactg cctgccgtct tcgcgcgcga aagaacgcgc ttcctgcgtg
    13321 agcgtttcct gaatactgcc gcgtatgtcc tgcaacacgg cggggttggc gcattccaac
    13381 gccttgggcg gttcttccct gccgcacgct gcaagcagca gggcaaacgg cagcgcaatg
    13441 agtttccgat acatgatgtt ccttccaaac ggtgcagacc gatggcggct atcatagcaa
    13501 aagcacctgc aggcggtaaa ccttgcaggc gctttgtcgg aaaggcggaa aatcagcctt
    13561 taaagaaatt cacaaaaccg gtgagaatcg cggcattaat caaatcgaca aagaacgcgc
    13621 cgaccatagg cacaatcaaa aacgccttat gcgacgcgcc gaaagtatgc gtgacggact
    13681 gcatatttgc caccgccgtc ggcgttgcgc ccaaaccgaa accgcagtgg ccggcagcca
    13741 atactgcggc atcatagtcg cgccccataa agacataggt aacaaaagtc gcgtacaaaa
    13801 ccatcactgc ggtttgtact gcaagaatca cggttacaga ccccgccaaa ccggtcagct
    13861 cccacagttt caaattcagc aacgccattg ccaagaaaag cgaaagcgaa gcattgccga
    13921 acacatcgat ggcacggtcg aacatattga ccttgaatgc ggcggtaagg atgttgcgga
    13981 taaccacgcc gccaaacaga caccacacga atttgggcag gtcgaacagg tattctttgt
    14041 cgaagccgtc cataatctcg gcaaacgcca gacacgcggc aaacatggca agcgtttcaa
    14101 cggcagattc cgccgtaatc aggcgggtgc gttttgcctg ctcgaacacg tcgtccgcgt
    14161 tgtcgtcctg atcctgtttt gtgttttcaa ccggtttgcg gcccattttg ttgatcaggc
    14221 ggcgcgcaac cggcccgccg atcaggccgc cgaacaccag cccgaaagta gccgaagcaa
    14281 tacccaaacc ggttgcgccg accaagccgt attgcgtttc aaaattaggt ccccacgcac
    14341 ctgacgtgcc gtgtccgccc gtcagcgaca ccgaaccggt aatcagaccg atgagcgggt
    14401 ccaaacccaa agccgtagcc agtccgaccc cgacaaagtt ttgcaccaag ataaatccgc
    14461 ccacaatcgc ggtaaaaacc accagcggca aaccgcccgc cttcaaacgg gaaaaatccg
    14521 cgctcaagcc gatggacgtg aagaaaatca gcataaacgc attttgcagc ggtttctcaa
    14581 atttgaagct cacgccgtac gcctcgtgca gcgcaaacag gataatcgcg gcaatcagcc
    14641 cgcccgccac gggttcgggg atgttaaaat cacgcaagat tttgattttc ttaaccaaaa
    14701 ccttgccgac caacaaaacc aaagtggcgg caatcaaggt gtaataactg ttaaacgccc
    14761 attccatatc gtttgacctc ctaaaatgtt tttctttata tccgcccgaa ggggacgcaa
    14821 gcgcgaagat attaggggaa tagttaaggc ttgtcaaaga atcgccgttt tttatagtgg
    14881 attaaattta aaccagtaca tcgttgactc gccttgccgt actatctgta ctgtctgtgg
    14941 cttcgtcgcc ttgtcctgat ttaaatttaa tccactatac aactattttt tacaaacaaa
    15001 atgccgtccg aaactccgtt cagacggcat catgggaata cacatatttg aaataacaga
    15061 aaaagcgggg gcaaaacccg ccgctgcatc tgtcgctgta catccctgct gtcggactta
    15121 ccgcttcaac ttcgcaaacg catccgccat cgccgaattg gttgggacac gctcgttgcg
    15181 ttgtggtttg cggccggctg tccgttcgcg gctgcggttt ttccagctcg gacagaatgt
    15241 ccagaatggt cggcagtccg aagtgttcgt cggtgaagtc ggacgctttg atttgcttca
    15301 cgcgctcgcg gttaactgcc taaatatgaa attcttggtc ttcttgtagc ataagttctc
    15361 gatgatatgc caagtaagat ttacgtttgt cgttgagcag attgttatct agcttcacat
    15421 cacaccaacg tcgccaaaca tcatctgata accgcttaga tttcaccata tttccttcat
    15481 catcaaacga aatatatttc aaatcaaata ggctatccaa ggtacgactt aatagcaagc
    15541 cgttatttgg gtcgtatgct tcggtatcat ccgacaggat gaaaggctta atatgactgg
    15601 caatcaatac aggataggca agtttttcca gcatacattt cacattaccg taatgttctt
    15661 cactttcttc ttgaagttgg tttttgtata ggcgatgtaa ataagggtcg cgtttcctta
    15721 cagtaatttc atccaaatta ccaaaaatac gttgggcatc ctcagcaaaa tataagtcat
    15781 cacctactcg ttttaagtcg tctaatttgt ctaataggtt ccataaataa cttatttgat
    15841 tgtattttcg ttcaataaat cctgattcta ttccctgttg aaaataatca ttcaattctg
    15901 tttcggttag ataatcttgt tgaaaggttt ttaagtctac taacatcatg gcggcaattt
    15961 cttttttatt tagcttgcct tgtggatgct cgaccagcgt tttaatcagg aaatttattt
    16021 gtcgaatatt agagttttca gttactgaac gctgaaagcc agagtgcgta tatacgattt
    16081 tggaaagtag ggtttgtcgt tttcggttag tacgagcttg tacataatct cgtgataaag
    16141 gtgtatagct tgataaaaac ggttctataa atcccatttt tacaaattga ttaatagctt
    16201 tacgaaccga ggccaaattc atggccttgc ttgttctctg cgaagccata acggaatctt
    16261 gggcaatgtt ttgctgcaat gcttcatata gttcgttgct gtatggctgg tcttgattcg
    16321 catcaataaa gtcaagacaa atattgagtg cagtacaaaa tttttgatcg ttataatttg
    16381 taaaggcatt ggtaaacgcc caataatctt cataaatttg gctgtttggc tgattattac
    16441 tcatctctgt ctccaagaat attcgatacc aatattctgt ttttgtattt aagtaatatc
    16501 tatcattcat gtattttcta ctaaaaacag aatttcctta tggctttgct tcttttcttt
    16561 tcctgtaacc ttataaacgt gaggcgtttc caagatttgt acatttcgcc catttttacc
    16621 taacatttct tctaattcat ctcggtttgg atatgaagca ttgttgtagc ttaacagcca
    16681 atatttaaat ggtttcaaat gattgattac ccttttaaat aattcgacag cctgcttttt
    16741 atccatgaaa tgattggcaa atggtttggg tatggatgat gtaatgtaac tgtccaataa
    16801 tccgtaaaag ccaaaataat tgttcatcgt gcctgtgtaa ggtgggtcta aatagacggc
    16861 atcagcttgt atattaggta ataaatcaaa tatatcttga ttaaaggcgt gatgccgcca
    16921 gccattattg aaaacagctt ggttatagtc atccaaattt tgcagaaaat gactttgaaa
    16981 actttcattg tggtaggcgc gtcgacggcc atatttagca taactgtatt cttcatcacg
    17041 cagttgttta actttttccc aaggaattgt gaatcgtgaa tagggcattt tacgtatcat
    17101 ggcgcgacgc attaaagtaa acgccaaagc acgtttatac tggttattca gcttccctat
    17161 atttttacgg tacaaatcaa gttgttggca ttcatcgtga aaatagaatt tttcggaata
    17221 acgctgactc ataaaaccgg catgcgggct gcctgaaaaa atcattgcga catcatcgtc
    17281 atttaatgtt tcatggttgt tttctattaa tgctaaagca atttggtaat taattgccaa
    17341 aatatcgtta gtaatcacac gatagccgcg ttttttggct tcgtaggcaa aggaacagcc
    17401 tccactaaat acatctgcaa ctgtatctac atcagacgga agctggtcac aaatccagga
    17461 agctattttc tctttattac cgatatagtt aattttcgga tatttcttat tcattttcac
    17521 tcatttttaa aatactttca gcaatagctt ttgccaataa aggcggcacg gcgttaccaa
    17581 cctgctgctg ctgggcaatt ttgctgccgc aaaaaataaa attatcaggg aaagattgta
    17641 aggcagctaa ttcacgaacg gttaaagccc gattctgttc atagtgaaaa actttgcgca
    17701 tatctcctgt aatacaaacg gctggtttgt tgctgttgta acggatgtat ttacggatat
    17761 cacctgtttt cggacgtaat ggttcaggaa tatcgttacg gttacctcca tttttaacaa
    17821 atgccatttt ttctaacatt tgtgccgaat gattcatagc ttcatgattt gcaacgtgtg
    17881 gattgctttc gccagcagcc agttttggaa aatgtcctat tgctgatcca acagtctgat
    17941 gggaaatctg caaaggttcg ggaaaggaaa ttttgccttt atccctcctc ccgataaata
    18001 tcactcggct acgtatctga ggaacaccga aatcggctgc actcagtatc ttacattcca
    18061 ccgaataacc gatattctga aatgcttgaa taatctcaat acgtgtttta cctgaattgt
    18121 gtgtatagag tcgcgctaca ttttccataa caaaaaaata tggttggaca attttaacta
    18181 ttcggacaaa ctctttaaat aaatggttgc gtgggtcatc tgtaaatgtc cgtccaatct
    18241 ttcctgccat actaaaacct tgacaaggtg gtcctccaat aaccaaatca actgattgtc
    18301 cgttaagaca attggttaaa tcttgttcgg ttagtgtggt taaatctttt tgcagtaatt
    18361 gatgacgggg gaagttggta cggtaagtct gacaataatc agactccatt tcaacagaaa
    18421 gcaattgttg gaatccggct tgttcaaaac ccaaggatag gcctcctgct cctgagaaaa
    18481 ggtcaatata ggtaagtggt gtgtgcataa gtcaaaatcc ataaactctt cgattatttt
    18541 accgcttcaa cttcgcaaac gcatccgcca tcgccgaatt ggtcggggcg cggtcgttgc
    18601 gttggggttt gcggccggct gtccgttcgc ggctgcggtt ttcagacggc attttatgtt
    18661 ttgcgccgcc cggttcgtca tccaagcgca tggtcagcgc gatgcgtttg cgtgcggcat
    18721 cgacttccag cactttcact tttaccacgt cgccggcttt caccacgtcg cgcgggtctt
    18781 ggacgaactt attagacagg gcggagatgt gcaccaagcc gtcctgatgg acgccgatgt
    18841 ccacgaacgc gccgaagttg gcgacgttgg agaccacgcc ctcgagtatc atgccgactt
    18901 gcaagtcgct gatttcgtgg atgccttcgg caaacgatgc cgtctgaaac gcaccgcgcg
    18961 ggtcgcggcc gggtttttcc agctcggaca ggatgtccaa aatagtcggc agaccgaagc
    19021 gttcgtcggt gaagtcggat gctttgattt gcttcacacg ctcgcggttg ccgatgagtt
    19081 cggcggcggt aatgccttgt tgcgccagca ttttggcgac gacgggatag gcttcggggt
    19141 ggacggcgct cgcgtccagc ggctctttgc cgccgttaat ccgcaaaaag cctgctgctt
    19201 gctcgaaggt tttttcgccc aagcgcggta cttttagcaa tttttttcgg ctgtcgaacg
    19261 cgccgttttc atcacggtag gcgacgatgt tttgggcaag ggtttgattc aagccggaaa
    19321 tccgcgccaa gagcggggcg gaggcggtgt tcgcgtccac gccgacggcg ttcacgcagt
    19381 cttcgaccac cgcgtccagc gatttggcga gccggctttg gttcacgtcg tgctgatact
    19441 gccccacgcc gatggatttg gggtcgattt tgaccaactc ggcaagcggg tcttgcagcc
    19501 tgcgggcgat ggacactgcg ccgcgcaggg aaacatctaa gtcgggaaac tcgcgcgccg
    19561 ccagttcgga cgcagaataa atcgacgcgc cggcttcgga aacgacgatt ttgtgcaggc
    19621 tgctttcggg cattccgcgc accagttcgc ccgcgatttt gtcggtttcg cggctggcgg
    19681 tgccgttgcc gatggcgatg agcttcacac cgtgttgttt aatcaggcgc gacaacgttg
    19741 ccaacatatt gttttcttga tgcaaataga caatgaccgt atccagcagt ttgcccgtgt
    19801 cgtccaccac ggcgcatttc acgccgttgc ggtagccggg gtcgagtccc aaagtggtca
    19861 gccgcccggc gggcgcggcg agcagcaagt ctttgagatt gcgggcgaac acggtaatcg
    19921 cgtcagtgtc ggcagcttct ttcaaacgat tgagagcttc gagttccagc gacaaaaaga
    19981 ttttcgcgcg ccaagtcagg cgcacggtgt cgcgcagcca tttgtggccg tctgaaacct
    20041 tgaagcggcg ggcgatgatt tgctcgtatt cgctttgctg cgtaatcggc gtgtcgtcgg
    20101 gctggtattt gagcgcgatg ttcaacacgc cttcgttgcg accgcgcaaa accgccagcg
    20161 cgcggtggct gggcatggcg cgcacgggtt cgcggcggtc gaaataatcg ctgaattttt
    20221 cgccttcggt ttctttgcct tcaacgactt gcgtgtggat ttcggcttcg ttccacagct
    20281 tgtcgcgcag cgtgccgatg agttccgcgt cttcggcaaa ctgctccatc agaatcgcgc
    20341 gcgcgccatc cagcgcggct ttggcgtcgg ggatgttttc gttcaggtag ccttgcgcgg
    20401 cggcttccac gtcctgcggc tgttcggcaa gcaacacgtc cgccagcggc tgcaaaccgt
    20461 gttcgcgagc gatttgcgct ttggtgcggc gtttgggttt gtagggcagg tacaggtctt
    20521 ccaacgcggt tttgttgtcg gcggcttcga tttgcgccct gaggtcgtct gaaagcttgc
    20581 cttgctcttc aatgcttttt aacacaacgg ccttgcgctc ttccaactcg cgcaggtatt
    20641 gcagccgctc ggcaagctgg cgcagctgcg tatcgtccag cccgcccgtg gcttctttac
    20701 ggtaacgcgc gataaagggg acggtcgcgc cgtcgtccaa aagctcgata gcggcattga
    20761 tttgcacggt agtggcggag agttcttggg agaggattcg ggtgatgttc ataaattgcc
    20821 tccgtgatgc cgtctgaaca gccgacggcg caatgtgggt aatcgtttgg aaaagatgaa
    20881 gataatactg atatactttg cccgatacaa tctgaaagga atgtttatga ccgtcctgat
    20941 taccggcggc accggcttta tcggttcgca caccgccgtc tcgctcgtcc aatccggtta
    21001 cgatgccgtg attttggata atctgtgcaa ctcgtctgcc gccgtcctcc cgcgccttcg
    21061 gcaaattacc ggcagaaaca taccgtttta tcagggcgac atccgcgact gtcagatttt
    21121 gaggcagatt ttttcagaac atgaaatcga atccgtcatc cattttgccg gtttgaaggc
    21181 agtgggggaa agcgttgccg agccgacaaa atattacggc aacaatgttt acggcagcct
    21241 ggtgctggcg gaagaaatgg cgcgcgcggg cgtgttgaaa atcgtattca gctcgtcggc
    21301 aaccgtttac ggcgatgcgg aaaaagtccc ctatacggaa gatatgcgcc cgggcgatac
    21361 cgctaatcct tacggtgcgt ccaaagcgat ggtggagcgg atgttaaccg acatccaaaa
    21421 agccgatccg cgttggagcg tgattttgtt gcgctatttc aacccgatcg gcgcgcacga
    21481 aagcggactt atcggcgaac agcccaacgg cgttcccaac aatcttttgc cctatatctg
    21541 tcaagtggct tcgggcaggc tgccgcaact gtcggtattc ggcggcgact atccgacccc
    21601 cgacggtacg ggaatgcgcg actacatcca tgtgatggat ttggcagaag ggcatatcgc
    21661 ggcaatgaag gcgaaaggcg gcgttgccgg cgtacatttg ttcaacttgg gttcgggacg
    21721 cgcctattcc gttttggaaa tcatccgcgc ctttgaggcc gcatccggtt tgcacattcc
    21781 ttaccgaatc caaccccgcc gcgccggcga cttggcgtgt tcctatgccg acccgtccca
    21841 taccaaacaa caaaccggct gggaaaccaa acgcggcttg cagcaaatga tggaagattc
    21901 gtggcgttgg gtcagccgca accccggcag atatggggat taaatgcaaa ccgcaggcaa
    21961 aaaaaacatc ctcgttaccg gcggcgcggg ttttatcggc tcggcagtcg tccgccatat
    22021 tatccaaaac acccgagatt ccgtcgtcaa cctcgacaag ttaacctacg cgggcaatct
    22081 cgaatcgctg accgacattg ccgataatcc ccgctacgct tttgagcaag tggatatttg
    22141 cgaccgcgcc gaactcgacc gcgtgttcgc gcaataccgg cccgatgccg tgatgcactt
    22201 ggcggcggaa agccacgtcg accgcgccat cggttcggca ggcgaattta tccgaaccaa
    22261 tatcgtcggc acattcgacc tgctggaagc tgcgcgcgcc tattggcagc aaatgccgtc
    22321 tgaaaaacgc gaagccttcc gtttccacca tatttctacc gatgaagtct atggcgattt
    22381 gcacggcaca gacgatttgt ttacagaaac cacgccatac gcgccgtcca gcccctactc
    22441 cgcctccaaa gccgccgccg accaccttgt ccgcgcgtgg cagcggactt acaggctgcc
    22501 ctccattgtc agcaactgtt ccaataatta cggcccccga caattccccg aaaaactcat
    22561 tcctttgatg attctgaacg cactttccgg caaaccactg cctgtgtacg gcgacggggc
    22621 gcaaatccgc gactggctgt ttgtcgaaga ccacgcgcgc gcgctgtatc aggtcgttac
    22681 cgaaggtgtt gtcggcgaaa cctacaatat cggcggacac aatgaaaaaa ccaatctcga
    22741 agtgatcaaa accatctgcg ccctcttgga agaactcgcc cccgaaaaac cggccggcgt
    22801 ggcacggtat gaagatttaa ttactttcgt acaagaccgc cccggccatg atgcccgcta
    22861 cgccgtcgat gcggcgaaaa tcaggcggga tttgggctgg ctgcctttgg aaacttttga
    22921 atccggcctc cgcaaaaccg tgcaatggta tttggacaac aaaacccggc ggcaaaacgc
    22981 ataaaacggc aactgccgtt cggaacgctt aggtacgggc aaatagtttt cagacggcat
    23041 cccgacgcaa tgccgtctga aaacccatcg caaaggaaga aggaaaagat gaaaggcatc
    23101 atactggcag gcggcagcgg tacgcgccta tatccgatta cgcgcggcgt atccaaacaa
    23161 ctgctgcccg tgtacgacaa accgatgatt tattacccct tgtcggtttt gatgctggcg
    23221 ggaatccgcg atattttggt aattaccgca cccgaagaca acgctgcctt ccaacggcta
    23281 ctcggcgacg gctccgactt cggcatccgc ctgcaatatg ccgttcaacc cagccccgac
    23341 ggcttggcgc aggcatttat catcggcgaa gaatttatcg gcaacggcaa tgtctgcctg
    23401 attttgggcg acaatatttt ctacggtcaa tcatttacgc aaacattgaa acaggccgcc
    23461 gccaagaccc acggcgcaac cgtattcggt tatcgggtta aagaccccga gcgtttcggc
    23521 gtggttgaat ttgacgaaaa cttcaacgcc ttatccatcg aagagaaacc gcaacagccc
    23581 aaatccgatt gggcggtaac cggcttgtac ttccacgaca accgcgccgt cgagttcgcc
    23641 aaacagctca aaccgtccgc acgcggcgaa ttggaaattt ccgacctcaa ccggatgtat
    23701 ctggaagacg gctcgctgtc cgttcaaata ttgggacgcg gtttcgcgtg gttggacacc
    23761 ggcacgcaag aaagcctgca cgaggcggct tccttcgtcc aaaccgtgca aaatatccaa
    23821 aacctgcaca tcgcctgcct cgaagaaatc gcctggcgta acggctggct gacgaaaaaa
    23881 gatgtggaaa cacgggcaaa gcctttggaa aaaaccgcct acgggcaata cctgctgcgc
    23941 ctgatcggca aataaatacc gtctgaacgc cccgacggct ttcagacggc atccaaaccg
    24001 tgccgcgccg gcaccttaac cgacctgccg cacggcgcgc ataaaaaatc aaaccgcaaa
    24061 aaaacagcgg taacgatacc gccaaaggac tttatggaca tcatcgacac cgccctcccc
    24121 gacgtaaaac ttttaaaacc gcaagtcttc acagacgggc gcggtttttt tatggaaaca
    24181 ttccgcgacg gatggttcaa agaaaatatt gccgatcgaa ctttcgtgca ggaaaaccac
    24241 tccaactcca gcaaaggcgt attgcgcggc ctgcactacc aaaccgaaaa cacccaaggc
    24301 aaactcgtac gcgtagttgt cggcgaagtg ttcgacgtgg ccgtcgatat gcgcgaaggt
    24361 tcgccgactt tcggcaaatg ggcgggcgca accttgtcgg cgcaaaaccg ataccagctt
    24421 tggatacccg aaggcttcgc acacggtttt tgcgttttgg gcgacgcggc ggaagtcgtg
    24481 tacaaatgca cggactatta caaccctgaa accgaacagg ttttaatatg gaacgacccg
    24541 gcaatcggca taggctggcc gcttcaaacc gcgccgctgc tttcgcccaa agaccttgcc
    24601 ggcaaaacgt gggcacaagc cgaaaagctc cgccttacgc tttcccgata aaaaatgccg
    24661 tccgaacgtt tcagacggca ttttctccga caggctacct acccgccttc agtacgcgct
    24721 gtgcaaagaa aaacatcccg gtaacgaaga acgccaagcc caaccaagaa gcggcggcat
    24781 cccagttttg cagattcaac gcgaacggcg catccgagcc gccgttggtc acagagaagg
    24841 caataataaa cgccataatt acaccaatca ggctttcacc gacaatcagg cctgccgcga
    24901 aaagcgtacc gatacggtcg gcgtttttca gacggccttc gcggttttcc gccttcttgc
    24961 caatgatgtg tttcaacacc gccgccaaca ccgcgcctgc cacgatgggc atattgacgg
    25021 acggcggcag ataaatgccc ataccgaccg caaggacggg cagggcaagt ttaccgcctg
    25081 atgatttttt caacaccaaa tcgacgacga ttaataccgc tccaatcgcg ataccggtaa
    25141 agatgtacgc ccattcgagg ttgtgggcga aaatgcccga cgcaatggtc gtcatcaaag
    25201 tcgcttgagg ggctgccaaa gcctgcgccg catccatgcc ttcgcgcggc attgcgccgg
    25261 taaagccgta ggcttcgtaa agcagttcca acacgggcga aataaccagc gcaccaacga
    25321 tacaaccgat aatcagggcg acttgctgcc gccaaggcgt agctttgagc aggtagccgg
    25381 ttttcaagtc ttgcaggttg tcgttggaaa tcgaagccac gcagattact gccgatccgc
    25441 aaaaaagcgt caacgccagt aagaatttgc ggttggcctc atccgccaac aaacctccgg
    25501 attcgcctac cagcagcaaa accagtgaaa taacgacgat ggacacgatg cccacgccgg
    25561 aaatcgggct ggaagacgag ccgaccaaac ctgccatata accgcaggcg gcggcgacca
    25621 aaaagccgat gacggaagcc aaaagcgtgc aaaccgccac caaaagccac gccatgccgc
    25681 ccgtaatgtg cgaatcgccg ataaagtggt aaaacgacac gcctagaata aacatcatag
    25741 acaacaccca gaaaatcatg gctttaggcg acaaatcctg ttcggcgcgt tctgtagcgg
    25801 gcgcaccgcc gccaaaactc ttgaacgaca tcttcatgcc ttccaccatt ggcttgagca
    25861 gcatcaaaag cgtccaaacc gccgcgatac ctatggttcc cgcaccgata aaacgcactt
    25921 tttccttcca cagcttcatc gcaaacgccg ccatttccat atcggaaggt tgcggaatgt
    25981 gtgaggagaa atacggcacg gcaatacccc aagcaatcga aatgcccagc aggatggcga
    26041 tgccgcccgt cagtccgacc aaataacccg cgcccaacaa ggccagtgaa aagcccatcg
    26101 gcagttggaa aattgccgta ccgcttttaa accaataact cgcgctatcg gcaatcacac
    26161 gcagacctcc ggcgcaaaag ctcatcaatc ccgccaacgc gccgccggcc gccagctctt
    26221 tgataccgct gccgccctga cggtcatccc cttcttcatg accgcccact ttcaaaattt
    26281 cagcagccgc cacaccttcc ggataaggca aatcgctttt caccaccatc gcgtaacgca
    26341 gcggaatggt gaaaatcacc cccaaaatcc cgccggcaat acataaaagc gtcgtctgcc
    26401 aaaacgggaa accgctccag tagcccgcca tcagcaaacc gggcaggacg aagatgatgg
    26461 ttgaaagcgt acccgcagcc gaggcttggg tctgcaccat gttgttttcc aaaatattgc
    26521 tgcctttgaa aaattttaaa accgccatcg aaatcaccgc cgccggaatc gacgaggcaa
    26581 aggtcagccc gactttcaaa ccgaggtaaa cgttagacgc agtaaaaatt acagtgatca
    26641 atgcgccgag tatcatgcct cggagcgtca gttcgcggta ttcttctacc gaaccggata
    26701 aagatttatt cattattctt cctttgacaa cagacgttca catattgttg gcatcacgcc
    26761 atgatgtcaa gttttaaaaa gaacagttaa aaacaattat cccaccctgc ccatacccaa
    26821 ttgaaaataa aaactatttc caaacgaaca aaaacagccg tatcagggag gttccccgat
    26881 acggctgctt gtttccgaac cttaaaatca atcaaacaaa tcgcgcagct tgtctaaaaa
    26941 cgatttcttg cgcggcgttt ggttttccaa gcccgtagaa atccgctcaa attcttccaa
    27001 aagctctttt tgacggtcgg tcaaattgac aggcgtttcg acaacaatat ggcagtacaa
    27061 atcgccggtc gcgctgctgc gtaaagattt gacaccctta cctttcacgc gcatcctcct
    27121 gccggtttgg gtttctttgg ggacggtgag cttgactttg ccgtccaagg tcggcacttc
    27181 taactccccg cccaaagcag ccgtggcaaa actaatcggc agttcgcaat gcaaatccaa
    27241 accatcgcgt tggaaaatct tatgcgcccg aatgcggacg gttacataca ggtcgccggc
    27301 aggcgcgccg tgcatacccg gcccgccttc accgctcaaa cggatacgct gcccgtcatc
    27361 gataccggcg ggaatattga cttccaccgt cttgaccgcc ttattccgcc ccacgccacg
    27421 gcatttgacg caaggttctt taatatgttt gcccgcaccg cggcaggtcg gacaagtctg
    27481 ctgcatacgg aaaatcgcct gctggatgtg caccgtaccc gaacctttgc aagtcgggca
    27541 ggtttccggg gatgcccccg gtttcgcgcc gctgccgttg cagacatcac acgcttcata
    27601 agtcggaata ttgatgcgtt tcttcacacc ttttgcggct tcttcaagcg tgatttcgat
    27661 accgacctgc acgtcctcac cctgataatc aggctgggcg cgccccgaac cacctccaaa
    27721 catttggctg aaaatatccc caaagtcaaa accctgcgca ccgccaaatc cgccaaaccc
    27781 tccgaagccc ccctgtccgc cgccttcaaa cgccgcatga ccatactggt cgtacatggc
    27841 acgcttttcc ttgtcggaca aagtttcata cgccttttgt acttctttaa acttctcttc
    27901 cgcctcttta ttgtcaggat tgcggtcggg atggtatttc atcgccaatt tacggtaggc
    27961 ttttttaatc tcatcatcgg tagctgctct tgccacaccc agcgtcgcat aaaaatcttg
    28021 attactcatt ttttcatcta aaaataaaat cacggcttaa aatgagggca attgcgcaaa
    28081 acacaagaca aacagactga gacctttgca aaaatcccct aaaatcccct aaattcccac
    28141 caaggcattt aggggatttc ccatgagcac cttcttccgg caaaccgccc aagccatgac
    28201 tgccaaacac atcggccgct tcccgctatt ggagttggac caggtgattg attggcagcc
    28261 gatcgaacaa tacctgaacc gtcaaaaaac ccgttacctc agagaccgcc gcggccgccc
    28321 cgcccatccc ctgt
//
LOCUS       DAWTQS010000020        27885 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.020, whole genome shotgun sequence.
ACCESSION   DAWTQS010000020 DAWTQS010000000
VERSION     DAWTQS010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 27885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27885
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.020"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(97..2139)
                     /gene="recG"
                     /locus_tag="WNA25_000888"
     CDS             complement(97..2139)
                     /gene="recG"
                     /locus_tag="WNA25_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFA9823675.1"
                     /translation="MMSPETQKQLKITDVSAKKLDKLNLHTAWDLVLHLPLRYEDETH
                     IMPIKDAPIGVPCQVEGEVIHQEVTFKPRKQLIVQIADGSGSVLFLRFIHFYASHQKQ
                     TAVGKRIRAVGEIKHGFYGDEMIHPKIRDAEGGGLAESLTPVYPTVNGLNQPTLRRII
                     QTALDVTPLHDTLPDALLGRLKLPRLAESLRLLHSPPPSFTIHQLSDGTLPAWQRLKF
                     DELLAQQLSMRLARQKRVSGTAAALRGDGTLTQALRQALPFALTDAQEKVVSEICRDM
                     AQTHPMHRLLQGDVGSGKTIVAALSALTAIESGAQVAVMAPTEILAEQHFIKFKQWLE
                     PLGIEVVCLFGSLRKKAKDEAKAKLADGSVKIAVGTHALFSDGVAFHNLGLSIVDEQH
                     RFGVAQRLALKNKGREVHQLMMSATPIPRTLAMSFFADLDVSVIDELPPGRTPIKTRL
                     VNNVRRAEVEGFVLGTCRKGRQAYWVCPLIEESETLQLQTAAETLARLQTALPEPNIG
                     LVHGRMKAAEKAEVMAEFAAGRLNVLVATTVIEVGVDVPNAALMVIEHAERMGLAQLH
                     QLRGRVGRGAGESVCVLLFAEPLGELAKARLKVIYEHTDGFEIARQDLNIRGPGEFLG
                     ARQSGVPMLRFAKLEEDLHLLEQARETAPMLIEQNPEIVEAHLARWLSGREGYLGV"
     gene            complement(2225..2668)
                     /gene="secB"
                     /locus_tag="WNA25_000889"
     CDS             complement(2225..2668)
                     /gene="secB"
                     /locus_tag="WNA25_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFA9823676.1"
                     /translation="MSEELQPVFSIERLYVKDLSLEVPHAPQIFLEQGDPEVDMRVST
                     GSQKLEDGYYDVDVTVTVTAKLDNERTMFLNEVTQSGIFRLENIPEEDVQLLLGVACP
                     NILFPYAREAVSGTVTRAGFPPVLLAPINFEAIYQQQQEAEAAGA"
     gene            complement(2690..2947)
                     /gene="grxC"
                     /locus_tag="WNA25_000890"
     CDS             complement(2690..2947)
                     /gene="grxC"
                     /locus_tag="WNA25_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFA9823677.1"
                     /translation="MQTVTMYTGPFCPYCAMAKRLLHAAGVGHIDEIRVDASPEAFAE
                     MQRLSGQRSVPQIFIGETHVGGFTDLYRLQQEGGLDGLLNP"
     gene            3166..4659
                     /gene="rng"
                     /locus_tag="WNA25_000891"
     CDS             3166..4659
                     /gene="rng"
                     /locus_tag="WNA25_000891"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFA9823678.1"
                     /translation="MLSGLPIPKDIARPPETILVNITPQETRVAVLEENNICELHIER
                     NSGHSLVGNIYLGVVRRVLPGMQSAFIDIGLERAAFLHIVDVLEQRRNPEETQRIEHM
                     LFEGQSVLVQVIKDPINTKGARLSTQISLAGRFLVHLPQEDHIGVSQRIEDDAERSSL
                     RERLDKLLPENACRGYIIRTNAENATDEQLQSDIDYLTKVWEHIQEQAKIRPPETLLY
                     QDLPLSLRVLRDMVGCDTQKILVDSTVNHGRMTRFAEQYVHGALGRIELFKGERPLFE
                     THNIEQEISRALQPRVNLNFGSYLIIESTEAMTTIDVNTGGFVGARNFDETIFRTNLE
                     ACHTIARELRLRNLGGIIIIDFIDMAQESHREAVLQELAKALAFDRTRVTLHGFTSLG
                     LVELTRKRSRENLNQVLCEPCPSCQGRGRLKTPQTVCYEIQREIVREARRYDAESFRI
                     LAAPNVIDLFLDEESQSLAMLIDFIGKPISLAVETAYTQEQYDIVLM"
     gene            complement(4694..5944)
                     /locus_tag="WNA25_000892"
     CDS             complement(4694..5944)
                     /locus_tag="WNA25_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HFA9823679.1"
                     /translation="MFVPLAMLAGMFSYYETFHETEALQDDLLRQAALYVGPDSKSET
                     LPEGDGDTRILVQMPQQEDPVVSLPAHLADGLHTLRADGDDDYYRAYIRTTEQGRIAV
                     MQENEYREDLAEDAARQSVLPLLAALPLMILLTVWITHKAMRPVRKLSQSLEQRRING
                     LPALSVYNIPSEIRGFVTAINLLLKRVDEDIRRRQRFVADAAHELRTPMTALSLQAER
                     LNNMPLPPDAGRQSAVLQQSIRRNKHLLEQLLALARSQSDETPLTKTTFGLQSRFRQV
                     LQELMPLVLEKRQDIGVAVGGDVEVSADETEIYTLVKTFADNAVRYTPNGGRIDLGFT
                     DEGKYLAVWVEDNGNGIPESECARVLDPFYRILGTEQQGAGLGLSIADTLAKKYGGYL
                     ELTDSRRFGHGLLIRALLDKETLK"
     gene            complement(6026..6655)
                     /locus_tag="WNA25_000893"
     CDS             complement(6026..6655)
                     /locus_tag="WNA25_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFA9823680.1"
                     /translation="MSASLKDGGYAVDWVKNGAQVAAAAAAQPYDLMLLDLGLPGRDG
                     LDVLSEIRAAGCTVPVLIVTARDDLYSRLNGLDGGADDYIVKPFDMAEFKARMRAVLR
                     RGSGQAQACLSNGALSLNPATYQVEIIAEGRQVALSNQEFSVLQALLARPGVILSRSD
                     SEDKVYGWGGEVESNAVDFLIHGLCKKLGKESIQNVRGVGWLMPRQDAV"
     gene            complement(6923..8236)
                     /locus_tag="WNA25_000894"
     CDS             complement(6923..8236)
                     /locus_tag="WNA25_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HFA9823681.1"
                     /translation="MLTFIGLLIIGVIVWLLLTEKVSPIIALILVPLIGALLAGFDVS
                     QLKEFYSGGTKSVTQIVIMFMFSILFFGIMNDVGLFRPMIGGLIKLTRGNIVAVSVGT
                     VLVSVVAQLDGAGATTFLSVVPALLPLYKRLHMNPYLLFLLLTSSAGLINLLPRGGPI
                     GRVASVLGADVGELYKPLLTVQIIGVVFILVLSLFLGVREKRRIVRELGALPAVADLI
                     KPAPLSEEEQKLARPKLFWWNVLLFLAAMSLLFSGIFPPGYVFMLAATAALLLNYRSP
                     QEQMERIYAHAGGAVMMASIILAAGTFLGILKGAGMLDAISKDLVHILPDALLPYLHI
                     AIGVLGIPLELVLSTDAYYFGLFPIVEQITSQAGVAPEAAGYAMLIGSIVGTFVTPLS
                     PALWMGLGLAKLSMGKHIRYSFFWAWGLSLAILISSIAAGIVPLP"
     gene            complement(8614..9186)
                     /locus_tag="WNA25_000895"
     CDS             complement(8614..9186)
                     /locus_tag="WNA25_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFA9823682.1"
                     /translation="MIVAKKISILVGSLRRASFARKVALNAAEMFPEGWQAEIVEIGH
                     LPLYNFDYDDPAVEDVPLPESYTAFRETIKASDGILFVTSENNRTIPACLKNAVDIGS
                     KPNADVAWKNKPAGIISHSVGKMGGYSSQKNLRLALSYFDMPVTGQPEVFLGNSPTLF
                     DENGKLIDSARDFVQSYINQFVGLIERNAK"
     gene            9345..10754
                     /gene="dacB"
                     /locus_tag="WNA25_000896"
     CDS             9345..10754
                     /gene="dacB"
                     /locus_tag="WNA25_000896"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HFA9823683.1"
                     /translation="MNFPKTAASLLLLLASLAAHALDTGRIPQNEIAVYVQELDSGKV
                     IIDHRAGIPVNPASTMKLVTAFAAFKTFGSNYRWATEFKSNGTVNDGTLDGNLYWAGS
                     GDPVFNQENLLAVQRQLRDKGIRNITGRLMLDHSLWGEVGSPDHFEADSGSPFMTPPN
                     PTMLSAGMVMVRAERNAAGSTDILTDPPLPHIFAQNNLKITASQAACPSVKKLMRASF
                     SGNTLKLRGNIPESCLGKPVGVRMFALDELIRQSFTNRWLLGGGRISDGIGIADTPEG
                     AQTLAVAHAKPMKEILTDMNKRSDNLIARSVFLKLGGDGKLPAVSEQAASAVRRELAV
                     SGIDVADLVLENGSGLSRKERVTARMMAQMLETAYFSPFAQDFIDTLPIAGTDGTLRN
                     RFKQSGGLLRLKTGTLNNVRALAGYWLGDKPMAVVVIINSGRAVSLLPDLDNFVAKNI
                     ISGGDGWLDAKLMCKERRA"
     gene            complement(10819..11988)
                     /locus_tag="WNA25_000897"
     CDS             complement(10819..11988)
                     /locus_tag="WNA25_000897"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFA9823684.1"
                     /translation="MSEYLFTSESVSEGHPDKVADQVSDAILDAILAQDPKARVAAET
                     LVNTGLCVLAGEITTTAQVDYIKVARETIKRIGYNSSELGFDANGCAVGVYYDQQSPD
                     IAQGVNEGEGIDLNQGAGDQGLMFGYACDETPTLMPFAIYYSHRLMQRQSELRKDGRL
                     PWLRPDAKAQLTVVYDSETGKVKRIDTVVLSTQHDPAISHEELSKAVIEQIIKPVLPP
                     ELLTDETKYLINPTGRFVIGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVD
                     RSAAYACRYVAKNIVAAGLATQCQIQVSYAIGVAEPTSISIDTFGTGKISEEKLIALV
                     CEHFDLRPKGIVQMLDLLRPIYGKSAAYGHFGREEPEFTWERTDKAASLKAAAGL"
     regulatory      complement(12171..12281)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            12334..13227
                     /locus_tag="WNA25_000898"
     CDS             12334..13227
                     /locus_tag="WNA25_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFA9823685.1"
                     /translation="MFRLQFRLFPPLRTAMHILLTALLKCLSLLSLSCLHTLGNRLGH
                     LAFYLLKEDRARIVANMRQAGLNPDTQTVKAVFAETAKCGLELAPAFFKKPEDIETMF
                     KAVHGWEHVQQALDKGEGLLFITPHIGSYDLGGRYISQQLPFHLTAMYKPPKIKAIDK
                     IMQAGRVRGKGKTAPTGIQGVKQIIKALRAGEATIILPDHVPSPQEGGGVWADFFGKP
                     AYTMTLAAKLAHVKGVKTLFFCCERLPDGQGFVLHIRPVQGELNGNKAHDAAVFNRNT
                     EYWIRRFPTQYLFMYNRYKTP"
     gene            complement(13272..14336)
                     /gene="tsaD"
                     /locus_tag="WNA25_000899"
     CDS             complement(13272..14336)
                     /gene="tsaD"
                     /locus_tag="WNA25_000899"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFA9823686.1"
                     /translation="MLVLGIESSCDETGVALYDTERGLRSHCLHTQMAMHAEYGGVVP
                     ELASRDHIRRLVPLTEGCLAQAGASYGDIDAVAFTQGPGLGGALLAGSSYANALALAL
                     DKPVIPVHHLEGHLLSPLLAEEKPDFPFVALLVSGGHTQIMAVRGIGDYELLGESVDD
                     AAGEAFDKTAKLLGLPYPGGAKLSELAESGRPEAFVFPRPMIHSDDLQMSFSGLKTAV
                     LTAVEKVREANGSETIPEQTRNNICRAFQDAVVEVLEAKVKKALLQTGFRTVVVAGGV
                     GANRKLRETFGNMTVQIPTPKGKPKHPSEKVSVFFPPMAYCTDNGAMIAFAGAMHLGK
                     GREVGAFNVRPRWSLSEIVK"
     gene            complement(14452..15639)
                     /gene="ccsB"
                     /locus_tag="WNA25_000900"
     CDS             complement(14452..15639)
                     /gene="ccsB"
                     /locus_tag="WNA25_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HFA9823687.1"
                     /translation="MTEHYKTLPEHELLIQKSLIRNLNLWDWVFAVLVFAATVFVQTR
                     SGMHMDIYETVMLWASAGIAVFLGWFFKPMRWFVPLSVLLAYAAVGLYGGNIKSAEIF
                     LLRYFLSSQSAIMWQCAFVFFALFAYISGAVLASVKNVPTNTLLGMGTVFAWVSAVAG
                     FTGLLVRWHESYLLRPDAGHIPVSNLYEVFILFLVITALMYLYYEGKFAVQKLGGFVF
                     GFMAVVVGFVLWYSVSREAHTIQPLIPALQSWWMKIHVPANFIGYGAFCISAMLGIAE
                     LVSLRAEEKGGKLWLPPSALIDEVMYKAIAVGFLFFTIATILGALWAADAWGRYWSWD
                     PKETWAFIVWLNYAVWLHLRLVAGWRGKVLAWWAIIGLFVTAFAFIGVNMFLSGLHSY
                     GTL"
     gene            complement(15632..17584)
                     /locus_tag="WNA25_000901"
     CDS             complement(15632..17584)
                     /locus_tag="WNA25_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HFA9823688.1"
                     /translation="MRFAVALLSLLGIASVIGTVLQQNQPQTDYLVKFGPFWTRIFDF
                     LGLYDVYASAWFVVIMMFLVVSTSLCLIRNVPPFWREMKSFREKVKEKSLAAMRHSSL
                     LDVKIAPEVAKRYLEVRGFQGKTVSREDGSVLIAAKKGTMNKWGYIFAHVALIVICLG
                     GLIDSNLLLKLGMLAGRIVPDNQAVYAKDFKPESILGASNLSFRGNVNISEGQSADVV
                     FLNADNGMLVQDLPFEVKLKKFHIDFYNTGMPRDFASDIEVTDKATGEKLERTIRVNH
                     PLTLHGITIYQASFADGGSDLTFKAWNLRDASREPVVLKATSIHQFPLEIGKHKYRLE
                     FDQFTSMNVEDMSEGAEREKSLKSTLNDVRAVTQEGKKYTNIGPSIVYRIRDAAGQAV
                     EYKNYMLPILQDKDYFWLTGTRSGLQQQYRWLRIPLDKQLKADTFMALREFLKDGEGR
                     KRLVADATKDAPAEIREQFMLAAENTLNIFAQKGYLGLDEFITSNIPKGQQDKMQGYF
                     YEMLYGVMNAALDETIRRYGLPEWRQDEARNRFLLHSMDAYTGLTEYPAPMLLQLDGF
                     SEVRSSGLQMTRSPGALLVYLGSVLLVLGTVFMFYVREKRAWVLFSDGKIRFAMSSAR
                     SERDLQKEFPKHVESLQRLGKDLNHD"
     gene            complement(17862..18485)
                     /locus_tag="WNA25_000902"
     CDS             complement(17862..18485)
                     /locus_tag="WNA25_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c4"
                     /protein_id="HFA9823689.1"
                     /translation="MRRLTLLAFVLAAGAVSASPKADVEKGKQVAATVCAACHAADGN
                     SGIAMYPRLAAQHTAYIYHQTIGIRDGKRTHGSAAVMKPVVMNLSDQDILNVSAFYAK
                     QQPKSGEANPKENPELGAKIYRGGLSDKKVPACMSCHGPSGAGMPGGGSEIQAYPRLG
                     GQHQAYIVEQMNAYKSGQRKNTIMEDIANRMSEEDLKAVANFIQGLR"
     gene            18692..19321
                     /locus_tag="WNA25_000903"
     CDS             18692..19321
                     /locus_tag="WNA25_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HFA9823690.1"
                     /translation="MNLFQNAKFFTTVNHLKDLPDTPLEIAFVGRSNAGKSSAINTLT
                     NHVRLAYVSKTPGRTQHINFFELQNGNFMVDLPGYGYAQVPEAVRAHWVNLLGDYLRH
                     RKQLIGLVLIMDARHPLKELDIRMLDFFHTTGRPVHILLSKADKLSKNEQIKTLSQVK
                     KLLKPYSDRQNISVQLFSSLKKQGIDEANRTVGSWFDAADAAASSPEEN"
     gene            complement(19442..21838)
                     /locus_tag="WNA25_000904"
     CDS             complement(19442..21838)
                     /locus_tag="WNA25_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HFA9823691.1"
                     /translation="MIKKILTTCFGLFFGFCVFGVGLVAIAILVTYPKLPSLDSLQHY
                     QPKMPLTIYSADGEVIGMYGEQRREFTKIGDFPEVLRNAVIAAEDKRFYRHWGVDVWG
                     VARAAVGNVVSGSVQSGASTITQQVAKNFYLSSEKTFTRKFNEVLLAYKIEQSLSKDK
                     ILELYFNQIYLGQRAYGFASAAQIYFNKNVRDLTLAEAAMLAGLPKAPSAYNPIVNPE
                     RAKLRQKYILNNMLEEKMITVQQRDQALNEELHYERFVRKIDQSALYVAEMVRRELYE
                     KYGEDAYTQGFKVYTTVRTDHQKAATEALRKALRNFDRGSSYRGAENYIDLSKSEDVE
                     ETVSQYLSGLYTVDKMVPAVVLDVTKKKNVVIQLPGGRRVALDRRALGFAARAVDNEK
                     MGEDRIRRGAVIRVKNNGGRWAVVQEPLLQGALVSLDAKTGAVRALVGGYDFHSKTFN
                     RAVQAMRQPGSTFKPFVYSAALSKGMTASTVVNDAPISLPGKGPNGSVWTPKNSDGRY
                     SGYITLRQALTASKNMVSIRILMSIGVGYAQQYIRRFGFRPSELPASLSMALGTGETT
                     PLKVAEAYSVFANGGYRVSSHVIDKIYDRDGRLRAQMQPLVAGQNAPQAIDPRNAYIM
                     YKIMQDVVRVGTARGAAALGRTDIAGKTGTTNDNKDAWFVGFNPDVVTAVYIGFDKPK
                     SMGRAGYGGTIAVPVWVDYMRFALKGKQGKGMKMPEGVVSSNGEYYMKERMVTDPGLM
                     LDNSGIAPQPSRRAKEDDEAAVENEQQGRSDETRQDVQETPVLPSNTDSKQQQLDSLF
                     "
     gene            21990..23105
                     /locus_tag="WNA25_000905"
     CDS             21990..23105
                     /locus_tag="WNA25_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HFA9823692.1"
                     /translation="MRLFKSLKNPKKTDAKLPKKSSGLNNRAAIGIDIDQHSIKMVQL
                     SGRSLNQIQLEKYVIAKLPKNIIQGNKVQNYDQLVTYLQQAYAKLGTSCKNIVASVPQ
                     NLATIEQLTYTAKDAELDLQGFVESSISEASSISLEEANYDYQVLSQSAVGEAVLSVA
                     SRKDEIEPLIDAFNAAGMKLSALDVDIFGQYNAYALWINHFAPELADEKVAIFGVYAA
                     QTYALVIQDGKILYKQETSVSAEQLNQLIQRTYQVTAEKAEEIINSPQKPSDYQESVA
                     NYFNQQITQEIQRVLQFYYTTQTADDMTDIKHILLTGEAVRQKGIAQTVASQTNADVQ
                     CVHPARYFANDLKTDEQQFELDAPTLTKAFGLAVRGL"
     gene            23108..23707
                     /locus_tag="WNA25_000906"
     CDS             23108..23707
                     /locus_tag="WNA25_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HFA9823693.1"
                     /translation="MNNLIKINLLPYREEMNKRKQQQFKTLMYGAVLTGVAAVAATYL
                     FIDNMINKQSEKNTLLEISIAHLDTELSEIQKLKQEKDAFLIKKNKIGELQIKRLQAA
                     KILDSLNEAVPGSTYLTSLDAVTADSYRLSGRTSSDNRVAAMMRAMPNTGIFKQPELL
                     SIKKNNSHQEFTLQATLQPIVKAAESKENPASGNAQEAN"
     gene            23708..24355
                     /gene="pilO"
                     /locus_tag="WNA25_000907"
     CDS             23708..24355
                     /gene="pilO"
                     /locus_tag="WNA25_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HFA9823694.1"
                     /translation="MASKSSKTNLDLNNLHLLNLPARLFIALLVVAAVLGLGYAGLFK
                     SQMESLEEYEAKETELKNTYKQKSIDAASLNNLRDELASIRSAFDIMLKQLPTDAEIP
                     NLVQELHQAGSSNGLRLDSVMPQPPVDDGPIKKLPYSISITGNYEQISQFTRDVGSLS
                     RIITLESLKIAQSPENGGNPDGKSSILNLSAIATTYQAKSVEELAAEAAQNAEQK"
     gene            24373..24918
                     /locus_tag="WNA25_000908"
     CDS             24373..24918
                     /locus_tag="WNA25_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HFA9823695.1"
                     /translation="MKHYALLISFLALSACSQSSEDLNEWMAQTRREAKAEIIPFQAP
                     TLPVAPVYSPPQLTGPNAFDFRRMETAKKGENAPDTKRIKETLEKFSLENMRYVGILK
                     SGQKVSGFIEAEGYVYTVGVGNYLGQNYGRIESITDDSIILNELIEDSTGNWVSRKAE
                     LLLNSSDKNTEQAAAPVAEQN"
     gene            24937..27132
                     /locus_tag="WNA25_000909"
     CDS             24937..27132
                     /locus_tag="WNA25_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HFA9823696.1"
                     /translation="MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKV
                     SFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVLEYADPLLSKISAAQNSSRARLV
                     LNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPAKQQAAAPFTESVV
                     SVSAPFSPAKQQAAASAKQQAATPAKQTNIDFRKDGKNAGIIELAALGFAGQPDISQQ
                     HDHIIVTLKNHTLPTALQRSLDVADFKTPVQKVTLKRLNNDTQLIITTTGNWELVNKS
                     AAPGYFTFQVLPKKQNLESGGVNNAPKTFTGRKISLDFQDVEIRTILQILAKESGMNI
                     VASDSVNGKMTLSLKDVPWDQALDLVMQARNLDMRQQGNIVNIAPRDELLAKDKAFLQ
                     AEKDIADLGALYSQNFQLKYKNVEEFRSILRLDNADTTGNRNTLVSGRGSVLIDPATN
                     TLIVTDTRSVIEKFRKLIDELDVPAQQVMIEARIVEAADGFSRDLGVKFGATGRKKLK
                     NETSAFGWGVNSGFGGGNKWEAQTKINLPVAAAANSISLVRAISSGALNLELSASESL
                     SKTKTLANPRVLTQNRKEAKIESGYEIPFTVTTASGGGNSTNTELKKAVLGLTVTPNI
                     TPDGQIIMTVKINKDSPAQCASGSNTILCISTKSLNTQAMVENGGTLIVGGIYEENNG
                     NTLTKVPLLGDIPVIGNLFKTRGKKTDRRELLIFITPRIIDTAGNSLRY"
BASE COUNT         7067 a         8049 c         6707 g         6062 t
ORIGIN      
        1 ctgcggcttc gccgccttgt cctgattttt gttaatccac tataaaaaat cagatttccg
       61 ttacactttt ttctaatatt tcagacggca ttttgctcac acacccaaat aaccttccct
      121 gccggaaagc caccttgcca aatgcgcttc gacgatttca gggttttgtt caatcagcat
      181 cggggcggtt tcgcgcgctt gttccaaaag gtgtaagtct tcttcgagct tggcgaagcg
      241 cagcataggc acgccgcttt ggcgcgcgcc gagaaattcg ccggggccgc ggatattgag
      301 gtcttggcgg gcgatttcga agccgtcggt gtgttcgtag atgactttca gccgcgcttt
      361 ggcgagttcg cccaagggtt cggcaaacag gaggacgcac acgctttccc ccgcgccgcg
      421 ccctacccgt ccgcgtaatt ggtgaagctg cgccaagccc atgcgctcgg cgtgttcgat
      481 gaccatcagg gcggcattgg gcacatctac gccgacttcg ataacggtgg tggcgaccaa
      541 gacgttcaga cggcctgcgg caaattccgc catgacttcg gctttttcgg cggccttcat
      601 gcgcccgtgt accagtccga tattgggttc gggcaatgcc gtctgaagcc gggcgagggt
      661 ttcggcggcg gtttgcagtt gcagggtttc gctttcttca atcaatgggc agacccaata
      721 tgcctgccgc ccttttcggc aagtgccgag gacgaagcct tcgacttcgg cgcggcggac
      781 gttgttgacg aggcgcgttt taatcggcgt gcgcccgggc ggcaattcgt cgatgacgga
      841 tacgtccaag tcagcgaaga agctcatggc gagcgtgcgc gggatgggtg tggcggacat
      901 catcagctga tggacttcgc gccctttgtt tttgagtgcg aggcgttgtg caacgccgaa
      961 acggtgctgt tcgtccacaa tgctcaagcc caaattgtga aacgccacgc cgtctgaaaa
     1021 cagggcgtgc gtgccgacgg cgattttgac gctgccgtcg gcgagtttgg ctttggcttc
     1081 gtctttggct tttttgcgca aactgccaaa aaggcagaca acttcaatgc ccaaaggttc
     1141 gagccattgt ttgaacttga tgaagtgctg ttcggcaagg atttcagtgg gcgccattac
     1201 agccacttgc gcgcctgatt cgatggcggt cagcgcggat aaggcggcga cgatggtttt
     1261 gccgctgccg acatcgcctt gcagcaggcg gtgcatgggg tgggtttgcg ccatatcgcg
     1321 gcagatttcg gaaacaactt tttcttgtgc atcggtcagg gcaaacggca gggcttggcg
     1381 cagggcttgg gtcaatgtgc cgtcgccgcg caatgccgcc gccgtgccgc tgacgcgctt
     1441 ctgccgcgcc aaacgcatgg acaactgttg cgccaaaagt tcgtcgaatt tgagccgctg
     1501 ccacgccggc agcgtgccgt ctgaaagttg atgaatagtg aaactcggcg gcggcgaatg
     1561 caaaaggcgc aggctttcgg caaggcgcgg cagcttcaga cggcctaata aagcatcagg
     1621 caaggtgtcg tgcagcggcg taacgtccaa cgccgtctga atgatgcggc gcaaagtggg
     1681 ctggttcaaa ccgtttacgg tcgggtaaac cggcgtgagg ctttccgcca aaccgccgcc
     1741 ctcggcatcg cggattttgg gatgaatcat ctcgtcgccg taaaatccgt gtttgatttc
     1801 gcccacggcg cggatgcgtt tgccgaccgc cgtctgcttc tgatggctgg cgtaaaagtg
     1861 gatgaagcgc agaaaaagga cgctgccgga accgtcggca atttggacaa tcagctgctt
     1921 gcgcggtttg aacgttactt cctgatggat aacctcgccc tcgacctgac acggcacgcc
     1981 aatcggcgcg tccttaatcg gcataatgtg cgtctcgtcc tcgtaacgca gcggcaggtg
     2041 caacaccaaa tcccacgcgg tatggaggtt gagtttgtcg agcttcttgg cggaaacatc
     2101 ggtgattttg agctgtttct gggtttcggg cgacatcata ggcagattcc tttggacgcg
     2161 cctattttat ccgaaaacaa aaatgccgtc tgaaacggat tcagacggca tcggcaggca
     2221 ggaatcaagc cccggcggct tcggcttcct gctgttgttg gtaaatcgcc tcaaagttaa
     2281 tcggcgcaag caggacgggc gggaagccgg cgcgcgttac cgtaccggaa accgcttcgc
     2341 gcgcgtaagg gaagaggatg ttcggacacg ccacgcccaa cagcagctgc acatcttctt
     2401 cggggatgtt ttccagacgg aaaataccgc tttgggttac ttcgttcaaa aacatcgtgc
     2461 gctcgttatc caatttggcg gtaacggtta cggtaacgtc cacgtcgtag tagccgtctt
     2521 ccagcttttg actgccggtg gaaacgcgca tatccacttc gggatcgccc tgttccaaaa
     2581 agatttgcgg cgcgtgcggc acttccaaag acaagtcttt gacatacagt cgctcgatgc
     2641 tgaatacggg ttgcagttct tcgctcattt tgttttccta gtttgggggt taagggttca
     2701 gcagtccgtc cagcccgcct tcctgctgga ggcggtagag gtcggtaaat ccgccgacgt
     2761 gcgtttcgcc gatgaaaatc tgcggcacgc tgcgctgtcc cgaaagccgc tgcatttcgg
     2821 caaaggcttc ggggcttgca tcgacacgga tttcgtcgat atgtccgaca cctgccgcgt
     2881 gcagcagcct tttcgccatc gcgcagtagg ggcaaaacgg acctgtgtac atggtaacgg
     2941 tctgcatatt gggtttccga aagttttgca atgataatca atataggggc aatcgctccg
     3001 tttggcaagt gcggaacaga tgcacgttca gacggcatgt gcggagtgtg tcaaagtttc
     3061 ttttttaaag tatgataggc attgtgaaaa atatttttgc acccgctctg cgcggcgggg
     3121 cggaacggat gcaaaaatat ttttattaca ttttcaggaa aaaccatgtt gtcaggactc
     3181 cccatcccca aagacatcgc gcgcccgccc gaaacgatat tggtcaacat cacgccgcag
     3241 gaaacgcgcg tggcggtgtt ggaggaaaac aatatctgcg agctgcacat cgagcgcaac
     3301 agcgggcaca gcctggtcgg caatatctat ctgggcgtgg tgcgccgcgt gctgcccggg
     3361 atgcagagcg cgtttatcga catcggcttg gaacgcgcgg cgtttttaca catcgtcgat
     3421 gtcctcgaac aacgccgcaa ccccgaagaa acccagcgca tcgaacatat gctgtttgaa
     3481 gggcagtcgg ttttggtgca ggtcatcaaa gacccgatca acaccaaagg cgcgcggctt
     3541 tccacccaaa tctcgctggc ggggcgtttc ctcgtccacc ttccgcaaga agaccacatc
     3601 ggcgtgtccc aacgcatcga agacgatgcc gaacgcagca gcctgcgcga acgcctcgac
     3661 aagctcctgc cggaaaatgc ctgccggggc tacatcatcc gcaccaacgc cgaaaacgcc
     3721 accgacgaac agctccagtc cgacatcgac tacctgacca aagtgtggga acacatccaa
     3781 gaacaggcga aaatccggcc gccggaaacc ctgctttatc aggatttgcc tttaagcctg
     3841 cgcgtgttgc gcgatatggt cggctgcgac acgcagaaaa tcctcgtcga ttccaccgta
     3901 aaccacgggc gcatgacgcg ttttgccgaa caatacgtcc acggcgcatt gggcaggata
     3961 gaattgttca aaggcgaacg cccgctgttt gaaacccaca acatcgaaca ggaaatcagc
     4021 cgcgccctgc aaccgcgcgt caacctcaac ttcggcagct acctgattat cgagtccacc
     4081 gaagccatga ccaccatcga tgtcaacacc ggcggcttcg tcggcgcgcg caatttcgac
     4141 gaaaccatct tccgcaccaa cctcgaagcc tgccacacca tcgcccgcga attaaggctg
     4201 cgcaaccttg gcggcatcat catcatcgac ttcatcgata tggcgcagga aagccaccgc
     4261 gaagccgtgt tgcaggagct tgccaaagcc ctcgccttcg accgtacccg cgttaccctg
     4321 cacggtttta ccagcctggg gctggtcgag ctgacgcgca aacgctcgcg cgaaaactta
     4381 aaccaagtcc tctgcgaacc ctgcccttcc tgccaaggca ggggccgcct gaaaacgccg
     4441 caaaccgtat gctacgaaat ccagcgcgaa atcgtccgcg aagcgcgccg ttacgatgcc
     4501 gaaagtttcc gcatcctcgc cgcccccaac gtcatcgatt tgtttttgga cgaagaatcg
     4561 caatccttgg caatgctgat agatttcatc ggcaaaccga tttctttggc ggtcgaaacc
     4621 gcttacacgc aggaacaata cgatattgtg ctgatgtaaa aaatgccgtc tgaagccttc
     4681 agacggcatc tgtctatttc agggtttcct tgtccaacag cgcgcgtatc aacagcccgt
     4741 gtccgaaacg tcggctgtcg gtcagttcca aatatccgcc gtattttttg gccagcgtgt
     4801 cggcaatcga cagccccagc cccgccccct gctgctccgt tcccaaaata cggtaaaacg
     4861 gatcgaggac gcgggcgcat tcggattcgg gaatgccgtt cccgttatcc tccacccaca
     4921 cggcgagata tttcccttcg tccgtgaaac ccaaatctat cctgcccccg ttcggcgtgt
     4981 aacgtaccgc gttgtcggca aaggttttaa ccagcgtata gatttccgtt tcgtcggcag
     5041 acacttcgac atcgcctccg accgccacac cgatgtcctg acgtttttcc aaaaccagcg
     5101 gcatcagttc ctgcaacact tggcggaaac ggctttgcag accgaatgtc gttttcgtca
     5161 aaggggtttc gtccgactgc gaacgcgcca gtgccaaaag ctgttcgagc aggtgtttgt
     5221 tgcgcctgat gctctgctgc aaaacggcgg actgccgccc cgcatcgggt gggagcggca
     5281 tattgttgag ccgttccgcc tgaagggaaa gggcagtcat cggcgtacgc aattcgtgtg
     5341 ccgcgtcggc gacaaaccgc tgacggcggc gtatatcttc atcaacacgt ttcaaaagca
     5401 gattgatggc ggttacgaac cctctgattt cactgggaat attgtacaca ctcaaagcag
     5461 gcaggccgtt gattcggcgt tgttcgagac tttgcgacaa tttgcggacg gggcgcatgg
     5521 ctttgtgcgt aatccacaca gtcagcagta tcatcagcgg cagtgccgcc aacaggggca
     5581 acacgctttg ccgtgccgca tcctccgcca aatcttcacg gtattcgttt tcctgcatga
     5641 cggcaatccg tccctgttcg gtcgtgcgga tataggcgcg gtaataatcg tcgtccccgt
     5701 ccgcccgaag cgtgtgcaga ccgtccgcca gatgcgcggg caggctgaca acagggtctt
     5761 cctgctgcgg catctgtacc aaaatacgcg tatcgccgtc gccttcgggc aaagtttcgg
     5821 atttggaatc agggccgaca tacaatgccg cctgacggag caggtcgtcc tgcaacgctt
     5881 ccgtttcgtg gaaggtttcg tagtaggaaa acatacctgc aagcattgcc agcggaacaa
     5941 acatccaaat cagggcaagg ctgattctga cctgaagcga atgttttaaa attttaaaaa
     6001 aacggtccgg catcttcgtt cctgttcaga cggcatcttg acgcggcatc agccagccga
     6061 caccgcgcac attttgtatg ctttccttac ccaatttctt gcacagcccg tgaatcagaa
     6121 aatccaccgc attgctttcg acttccccgc cccaaccgta aaccttgtcc tccgaatccg
     6181 agcgcgacaa aatcacaccc ggcctcgcca gcaaagcctg caataccgaa aactcctggt
     6241 tgctcaatgc cacctgcctc ccctcggcaa taatttctac ctgatacgtt gcaggattga
     6301 gggataacgc accatttgac agacacgcct gcgcctgtcc gctcccgcgc cgcaacaccg
     6361 cccgcatccg cgccttaaac tccgccatat cgaacggctt gacgatatag tcatccgcac
     6421 cgccgtccaa accgttcagc cggctgtaca aatcatcccg cgccgtaacg atcagcacgg
     6481 ggacggtaca gcctgccgcg cgtatttccg acaaaacatc caaaccgtcc cgcccaggca
     6541 aacccaaatc cagcagcatc aagtcataag gctgggcagc ggcagccgcc gcaacctgcg
     6601 cgccgttttt gacccaatcc accgcatagc cgccgtcttt caaacttgcc gacaccgctt
     6661 acgcaatcat cgcattgtct tccaccagca aaacacgcat caactttccc ttcaaaataa
     6721 accgtgccta ttctaacacc ccaaaattag ccgcaattta gcggccttta cgcttgccgg
     6781 tatttttcaa aactgcggca caaaaaaacc gcgccggcaa ctgccttcag acggcattgg
     6841 ggcgcgattg caacacacgg acaggcaggc aaagcctgcg acagatcaca ggaacgattc
     6901 gggcttcagg cggcttcgcc gtttacggca gaggcacgat tcctgccgct atcgaactga
     6961 tcaatatcgc cagcgacaaa ccccacgccc agaaaaacga ataacggatg tgtttgccca
     7021 tcgacaattt cgccaaaccc aaacccatcc acaaagccgg cgaaagcggc gtaacaaaag
     7081 taccgacgat actgccgatc aacatcgcat agcctgccgc ttcgggtgca acgcccgcct
     7141 gcgaggtaat ctgttccaca atcggaaaca gtccgaaata ataagcgtcc gtactcaaaa
     7201 ccaactcaag cggaataccc aacacaccga tggcaatatg cagataaggc agcaacgcgt
     7261 ccggcaggat atgcacaagg tctttggaaa tcgcgtccaa catccccgcg cccttcaaaa
     7321 tccccaaaaa cgtacctgcc gccaaaataa tggacgccat catcaccgcg ccgccggcgt
     7381 gggcataaat ccgctccatc tgttcctgcg ggctgcggta attcaaaagc aacgccgccg
     7441 ttgcagccag cataaataca taacccggcg ggaagatgcc cgaaaaaagc aggctcatcg
     7501 ccgccaaaaa cagcaggaca ttccaccaaa acagtttcgg acgcgccaat ttttgttctt
     7561 cttccgacaa aggcgccggc tttatcaaat ccgccacggc gggcaacgcg cccaactccc
     7621 ggacaatccg ccttttttca cgcacaccca aaaacaggga cagcacaagg ataaacacca
     7681 caccgataat ttgcaccgtc aacaaaggtt tatacaattc gcccacatct gcgcccaaca
     7741 cgcttgcaac ccgcccgatc ggcccgcccc gcggcaaaag gttgattagc cccgcgctgg
     7801 aagtcagcag caaaaacagc aggtaaggat tcatatgcag acgcttgtaa agcggcaaaa
     7861 gggcggggac gaccgataaa aacgtcgtcg cgcccgcccc gtccaactgt gccaccaccg
     7921 acaccaagac cgtccccaca ctcactgcca cgatattacc ccgagtcagc ttaatcaaac
     7981 cgcctatcat cggacggaac agccccacat cgttcatgat tccaaaaaac aaaatggaaa
     8041 acataaacat aatcacaatc tgcgtcaccg atttcgtgcc gcccgaataa aattctttta
     8101 attgggatac atcaaacccc gccagcaacg ccccaatcag cggcaccaag attaatgcga
     8161 tgatgggcga cactttttcc gtcagcaaca gccatacgat gaccccgata atcagcaatc
     8221 cgataaacgt cagcattatt tctcctttat tttattttga acataaaacc gcccgtgcag
     8281 gcaaaaccgc caacatatgc gggataagcc ttgcattcca ttcacgcctg ccgacatttc
     8341 tgcatccgtc cgtcatttca cggcggcaac cgatacggaa caaccggcaa accggtatcg
     8401 ggacggcgcg ggggcatccg tcccggtgca ccgattcaaa cgaaactgcc ccctatcatt
     8461 gcggagcgcg gggcgtgccg tacacgcggg attttaaaca cgccgatcat ttgccgcgcg
     8521 ttatccggca aacggcaaac cttgacgctg cccggcccat ataaaaaagc cgcaaaaccc
     8581 gaaccggttt tgcggctttt cgatttggtt tgtttatttg gcatttcttt caatcaaacc
     8641 gacaaactga ttgatatatg actgaacaaa atcccttgcc gagtcaatca acttaccgtt
     8701 ttcatcaaac agcgtcggcg aattgcccaa aaatacttcc ggctgtccgg ttacgggcat
     8761 atcaaaatac gacagggcaa ggcgcaggtt tttttgggaa ctgtaaccgc ccatcttgcc
     8821 gacggaatgg ctgatgatgc cggccggttt gtttttccaa gccacgtcgg cattcggttt
     8881 cgagccgatg tccaccgcat ttttcaaaca ggcgggaatg gtgcggttgt tttcggacgt
     8941 aacgaacaaa atgccgtccg aagccttaat cgtttcgcgg aaagccgtgt agctttcggg
     9001 cagcggcaca tcttccaccg cagggtcgtc ataatcgaaa ttgtaaagcg gcagatgtcc
     9061 gatttcgacg atttccgcct gccagccttc ggggaacatc tccgccgcat tcaatgccac
     9121 tttgcgcgca aacgaagcac ggcgcaggct gcccaccaaa atactgattt tcttagccac
     9181 aatcattcct cctgaatatt aagtttgtgc gtttcaatca tttcataatg gtaacgatta
     9241 ttatatatgt gatttcccct gcaaacaagc cggtccgccg ccccggcgtt cccacttatc
     9301 cggctttgcc ttataattgc tttttttatg taacagattc acctatgaat ttccccaaaa
     9361 cagcggcctc cctgctgctg cttctcgcct ccctcgccgc acacgcgctc gataccggcc
     9421 gcattccgca aaacgaaatc gctgtatatg tccaagagct tgacagcgga aaagtcatca
     9481 ttgaccaccg tgccggcata cccgtcaatc ccgcgtccac gatgaagctc gttaccgcgt
     9541 ttgccgcctt caaaaccttc ggcagcaatt accgctgggc gaccgagttt aaaagcaacg
     9601 gtacggtaaa cgacggcacg cttgacggaa acctgtattg ggcgggcagc ggcgaccccg
     9661 ttttcaatca ggaaaacctg cttgccgtcc aacgccagtt gcgcgacaaa ggcatccgca
     9721 atatcacggg gcgcctgatg ctcgaccaca gcctgtgggg cgaagtcggc agtcccgacc
     9781 attttgaagc cgacagcggt tcgccgttta tgacgccccc aaatccgact atgctgtctg
     9841 ccggtatggt tatggtgcgc gccgaacgca atgccgccgg cagtaccgac atcctcaccg
     9901 atccgccttt gccgcatatt tttgcccaaa acaacttgaa aattaccgcc tcccaagctg
     9961 cctgcccttc ggtcaaaaaa ctgatgcgcg catctttttc gggcaatacg ctgaaattgc
    10021 gcggcaatat tcccgaaagc tgtttgggca agcctgtcgg tgtccggatg ttcgcgcttg
    10081 acgaactgat ccgccaaagt tttaccaacc gctggctgct cggcggcgga cggatttcag
    10141 acggcatcgg catagccgac acaccggaag gcgcgcagac gcttgccgtt gcacacgcca
    10201 aaccgatgaa ggaaattttg acggacatga acaagcgttc ggacaatctg attgcgcgtt
    10261 ccgtcttcct caaactcggc ggcgacggca aactgcccgc cgtttccgaa caggcggcgt
    10321 ctgccgtccg gcgagaactt gccgtatcgg gcatcgatgt tgcggatttg gttttggaaa
    10381 acggttcggg tctgtccaga aaagaaaggg taacggcgag aatgatggcg caaatgttgg
    10441 aaacggctta tttcagcccg tttgcacaag atttcatcga cacgctgccc atcgccggca
    10501 cagacgggac tttacgcaac cgcttcaaac aaagcggcgg gctgttgcgc ttaaaaaccg
    10561 gcacgctcaa caatgtccgc gcccttgcag gttattggct gggcgacaaa ccgatggcgg
    10621 tggtcgtcat catcaacagc ggccgcgccg tttccctgct gcccgacttg gacaacttcg
    10681 ttgccaaaaa catcatctcc ggcggcgacg gctggctgga tgcgaaactg atgtgcaaag
    10741 aacgccgcgc ctgaatcggg aaaatataaa aatgccgtct gaactgttcg gacggcattt
    10801 ttgattttca aaccggaatt acagccccgc tgccgctttc aatgaggccg ctttgtcggt
    10861 gcgctcccaa gtaaactcag gttcttcgcg gccgaaatgt ccgtaggcgg cagattttcc
    10921 gtaaatcggg cgcaagaggt cgagcatttg gacgatgcct ttgggacgca ggtcgaaatg
    10981 ttcgcaaacc agcgcaatca gtttttcctc gctgatttta cccgtaccga aagtatcgat
    11041 ggaaatcgaa gtcggttcgg caacgccgat ggcgtaggaa acttggattt ggcattgggt
    11101 tgccaagcct gcggcgacga tgtttttcgc cacataacgg caggcgtaag cggcggaacg
    11161 gtccactttg gacgggtctt tgccggagaa tgcgccgccg ccgtgcggag ccgcgccgcc
    11221 gtaggtatcg acaatgattt tgcggccggt caaaccgcag tcgccttgcg ggccgccgat
    11281 gacgaagcgg ccggtcgggt tgatcaggta ttttgtttcg tcggtcagca gttcgggcgg
    11341 caaaacgggc ttgataatct gctcaatcac ggctttactc agttcttcat ggctgatggc
    11401 aggatcgtgc tgggtggaca ggacgacggt atcaatgcgt ttcaccttgc cggtttcgct
    11461 gtcgtaaacc acggtcagtt gggcttttgc gtcagggcgc agccaaggca ggcggccgtc
    11521 tttgcgcaat tcgctttgac gctgcatcag gcggtggctg taatagatgg caaacggcat
    11581 cagggtcggg gtttcgtcgc aggcatagcc gaacatcaag ccttggtcgc ccgcgccttg
    11641 gttcaggtcg atgccttcgc cttcgttcac gccttgggcg atgtcggggg attgttggtc
    11701 gtagtacacg ccgaccgcgc agccgttggc gtcaaagccc agctcggagg agttgtagcc
    11761 gatgcgtttg atggtttcgc gtgcgacttt gatgtagtcc acttgggcgg tggtggtgat
    11821 ttcgcccgcc aatacgcaca agcctgtgtt gaccaaagtt tccgccgcga cacgcgcttt
    11881 ggggtcttgc gccaagatgg catccaaaat cgcatcggat acttggtcgg ctactttgtc
    11941 cggatggcct tcggaaaccg attcggaagt aaacagatat tcgctcattg aaattttcct
    12001 tgaaaaacaa accatcttct tcagacagca tgttgtatga acataatgtc gacagcggga
    12061 aatatagcaa aatttcccta ttcgtgccat tcagttgaga aatattccta tttaaacggc
    12121 gctttggaat ctctgtccgt acgtttcgta caggcaaaaa aattcccgca tcaagcgggt
    12181 ttggattgct ccggcgagcc acatcggttt ttcaaccgtc caccttacct ttccgtttga
    12241 aaagcaggtt ggcatggaat tcccaactct taatgcagcg cgcatcgtag cagaaaaggc
    12301 atattgccgc aatacttccc tttttcagac ggcatgtttc gtttacaatt caggctgttt
    12361 ccccctttgc gaaccgccat gcacatcctg ttgaccgccc tgctcaaatg cctctccctg
    12421 ctgtcgcttt cctgtctgca cacgctggga aaccggctcg gacatctggc gttttacctt
    12481 ttaaaggaag accgcgcgcg catcgtcgcc aatatgcggc aggcgggttt gaaccccgac
    12541 acgcagacgg tcaaagccgt ttttgcggaa acggcaaaat gcggtttgga acttgccccc
    12601 gcgtttttca aaaaaccgga agacatcgaa acaatgttca aagcggtaca cggctgggaa
    12661 cacgtgcagc aggctttgga caagggcgaa gggctgctgt tcatcacgcc gcacatcggc
    12721 agctacgatt tgggcggacg ctacatcagc cagcagcttc cgttccacct gaccgccatg
    12781 tacaagccgc cgaaaatcaa agcgatagac aaaatcatgc aggcgggcag ggtgcgcggc
    12841 aaaggcaaaa ccgcgcccac cggcatacaa ggggtcaaac aaatcatcaa ggccctgcgc
    12901 gcgggcgagg caaccatcat cctgcccgac cacgtccctt ctccgcagga aggcggcggc
    12961 gtgtgggcgg attttttcgg caaacctgca tacaccatga cactggcggc aaaattggca
    13021 cacgtcaaag gcgtgaaaac cctgtttttc tgctgcgaac gcctgcccga cggacaaggc
    13081 ttcgtgttgc acatccgccc cgtccaaggg gaattgaacg gcaacaaagc ccacgatgcc
    13141 gccgtgttca accgcaatac cgaatattgg atacgccgtt ttccgacgca gtatctgttt
    13201 atgtacaacc gctataaaac gccgtaacga aaataaaaat gccgtctgaa caggtttcag
    13261 acggcatctt gtcatttgac gatttcggac aacgaccaac gcggacggac gttgaacgca
    13321 ccgacctccc tgcccttgcc caggtgcatc gcaccggcaa aggcaatcat ggcaccgttg
    13381 tccgtgcagt atgccatcgg cgggaaaaac acgctgactt tttcggacgg atgtttcggc
    13441 ttgcctttgg gggtcgggat ttgtaccgtc atgttgccga aagtttcacg gagtttgcgg
    13501 tttgcaccga ccccgccggc gaccactacg gttctgaacc ctgtctgcaa cagggctttt
    13561 ttcacttttg cctccaacac ttctactacc gcatcttgaa acgcacggca aatattgttg
    13621 cgggtttgct cgggtatggt ttccgaaccg tttgcctcac gcactttttc aacggcagtc
    13681 aaaacagcgg ttttcaatcc cgaaaaactc atctgcaaat cgtcggaatg aatcatcggg
    13741 cgcggaaaaa caaacgcttc gggcctgccc gattccgcaa gttccgacag tttcgcaccg
    13801 cccggatacg gcaaacccag cagtttcgcc gttttgtcga atgcctcgcc cgccgcatca
    13861 tcgacgctct cgcccaaaag ctcgtaatcg cctatgcccc tgaccgccat aatctgcgta
    13921 tgcccgcccg aaaccaacag cgcgacaaaa ggaaagtcgg gtttttcctc cgccaacagc
    13981 ggcgacagca gatgtccttc caaatgatgg acggggatga cgggcttatc caacgctaaa
    14041 gccagcgcgt tggcgtagct cgaacccgcc agcagcgcac cgcccaaacc gggcccctgc
    14101 gtaaaggcaa ccgcgtcaat atcgccatac gatgcgcctg cctgcgccaa acagccttca
    14161 gtcaacggaa caaggcggcg gatatggtcg cggctcgcca attccggcac gaccccgccg
    14221 tattccgcgt gcattgccat ttgagtgtgc aggcagtgcg accgcaatcc tcgttcggtg
    14281 tcgtacagcg caacgccggt ttcgtcgcaa gaagattcga ttcctaatac caacatggtc
    14341 tgatgccgtt aaaaactgaa aaacgtattc tagcgaattt cggcacgact gccgtatccc
    14401 caaaacggaa catgccgtct gaagcccttc agacggcatc gtcgcaccgt atcaaagcgt
    14461 tccgtaagaa tgcagcccgc tcaaaaacat attcacgccg ataaaggcga atgcggttac
    14521 gaacaaaccg ataatcgccc accacgccag cactttgccg cgccagcccg ccaccagccg
    14581 caaatgcagc cacacggcgt aattcaacca aacgatgaac gcccaagtct ctttcggatc
    14641 ccagctccaa tagcgtcccc aagcgtccgc cgcccacaac gcgcccaaaa tggtggcaat
    14701 ggtaaagaac aaaaagccga cggcaatcgc cttatacatc acctcgtcga tcaatgccga
    14761 cggcggcagc cacagttttc cgcctttttc ttccgcacgc agggaaacca gttcggcaat
    14821 accgagcatc gcggaaatgc agaacgcgcc gtaaccgata aagtttgccg gaacgtggat
    14881 tttcatccac caggactgga gcgcgggaat cagcggctgg atggtatgcg cctcacgcga
    14941 cacgctgtac cacaacacaa agccgaccac gaccgccata aagccgaaca cgaagccgcc
    15001 caatttctgc acggcaaatt taccctcgta atacaaatac atcagcgcgg taatgaccaa
    15061 aaacagaatg aacacttcat aaaggttgga cacggggata tgccctgcat cgggacggag
    15121 cagatagctt tcgtgccaac gtaccagcag accggtaaag cctgctacgg cagacaccca
    15181 tgcaaacacg gttcccatac ccaacagcgt gttggtcggc acatttttca cgcttgccaa
    15241 aaccgcgccc gaaatatagg cgaacagggc gaagaagaca aaagcacact gccacatgat
    15301 cgccgattgg ctgctgagga aataccgcaa caggaaaatc tctgccgatt taatgttgcc
    15361 gccatacaga ccgacggcgg cataggcaag caatacgctt aaaggaacaa accagcgcat
    15421 cggtttgaaa aaccaaccca aaaacacggc aataccggca ctcgcccaca acatgaccgt
    15481 ttcgtaaatg tccatatgca taccggaacg ggtctgcacg aaaaccgtag ccgcaaaaac
    15541 cagcacggca aatacccaat cccaaagatt cagattgcgg atcaaagatt tctgaatcag
    15601 cagctcgtgt tccggaaggg ttttatagtg ttcagtcatg attcaagtcc ttgccgagcc
    15661 gttgcaggct ctcgacgtgt tttggaaatt ccttctgcaa atcccgttcg ctgcgggccg
    15721 aagacatagc aaaacggatt ttgccgtctg aaaacaatac ccacgcccgt ttttcgcgca
    15781 cataaaacat aaataccgta cccaaaacca acaataccga gccgagatag accaaaagcg
    15841 cacccggcga acgggtcatc tgcaaacctg aggaacgcac ctcggaaaac ccgtcaagct
    15901 ggagcagcat aggcgcggga tattccgtca gccccgtata ggcatccata ctgtgcagca
    15961 ggaaacggtt ccgcgcttca tcctgccgcc attcgggcaa gccgtaccgg cgtatggttt
    16021 catccaaagc agcgttcatc acgccgtaaa gcatttcgta gaaatagccc tgcatcttat
    16081 cctgctgccc tttcgggata ttggacgtaa taaattcgtc caatcccaaa tagccttttt
    16141 gcgcaaagat attcagcgtg ttttccgcag ccagcatgaa ttgttcgcgg atttcggcag
    16201 gtgcgtcttt ggttgcgtcg gcaaccagac gtttgcgccc ttccccatct ttcaaaaact
    16261 cacgcaatgc cataaaggtg tccgctttca actgcttgtc caaggggata cgcagccagc
    16321 ggtattgctg ctgcaagccg ctgcgcgtgc cggtcagcca aaaataatct ttgtcctgca
    16381 aaatcggcag catatagttt ttatattcga ccgcctgccc tgccgcatca cggatgcggt
    16441 acacgatgga agggccgata ttggtgtatt ttttaccttc ctgagtaacg gcgcggacat
    16501 cgttcagagt ggatttcagg cttttttccc gttccgcacc ctcgctcatg tcctccacat
    16561 tcatagaagt gaactgatcg aactcaagac gatatttgtg tttgccgatt tccaacggaa
    16621 actggtgtat ggaggttgcc ttcaacacga caggttcgcg cgaagcatcc ctcaaattcc
    16681 acgccttgaa tgtcaaatcc gaaccgccgt cggcaaaact cgcctgataa atcgtgatgc
    16741 cgtgcaaggt caaaggatgg ttcacgcgga tggtgcgctc gagtttctca ccggttgcct
    16801 tgtccgttac ttcaatatcg ctggcaaaat cgcgcggcat acccgtattg taaaaatcga
    16861 tatggaattt tttcagtttg acttcaaaag gcaagtcctg aaccaacatc ccgttgtcgg
    16921 cattcaggaa aaccacatcc gcactttgcc cctcggaaat attgacgttg cccctaaatg
    16981 agagattgga cgcacccaaa atactttcgg gcttgaaatc cttggcataa accgcctgat
    17041 tgtccggaac aatccgaccg gccagcatac ccagcttcag cagcaggtta ctgtctatca
    17101 acccgcccag gcaaatgaca atcaaagcaa catgggcaaa gatatagccc catttgttca
    17161 tcgtgccttt tttggcggca atcagaaccg acccgtcctc acggctgacg gtttttccct
    17221 gaaaaccccg cacctccaga taacgtttgg caacttcggg ggcaattttt acatccaaca
    17281 gcgaagaatg gcgcatcgcc gccagagatt tttctttaac cttttcccgg aaagacttca
    17341 tttcgcgcca aaacggcgga acgttacgga ttaaacacaa actggtagaa accaccagaa
    17401 acatcatgat aacgacaaac catgccgaag catagacatc gtacaaaccc aaaaaatcaa
    17461 aaatccgagt ccaaaacggt ccgaatttga ccaaataatc cgtctgcggc tggttttgct
    17521 gtaacaccgt gccgataacc gatgcaatac ccagcagact gagcaaagcg accgcaaagc
    17581 gcatggagct gaaaaaagcg aaccacggac gggaaagaag tgtgggggat atacgggatt
    17641 tactcattgt gtgtttattc cgccatcagg aatatgggaa agcagaattg ggcagacaga
    17701 aaacaacgtc ccgattctac tgtcttgatg ctttttattt caagacaatg aagacagcct
    17761 gcatcgattc caacggttgc gattgaaaaa acttatcgca aaattttcag aagccgtctg
    17821 aaaacctttt cagacggctt ctgaagcaga cccttggatg attaacgcaa accctggata
    17881 aagttagcaa ctgcttttaa atcttcttcg gacatacggt ttgcaatatc ttccatgatg
    17941 gtatttttac gctgaccgga cttgtaggca ttcatctgtt caacaatata tgcctggtgc
    18001 tggccgccca aacgcggata agcctgaatt tcgcttccgc ctcccggcat acccgcaccg
    18061 ctcggaccgt ggcaggacat acacgccggc acttttttat cgctcaaacc gccgcgatag
    18121 attttcgcgc ccaattcggg attttcctta ggattggctt cgccggattt gggctgctgt
    18181 ttggcataga atgcggatac gttcaaaata tcctgatcgc tcaaattcat taccaccggt
    18241 ttcatcacag ctgccgaacc gtgggtgcgt ttgccgtcgc ggatgccgat agtttgatga
    18301 tagatgtaag cagtatgctg tgccgccaaa cgcggataca tcgcaatgcc gctgttaccg
    18361 tctgctgcat ggcaagccgc acaaaccgtt gcggcaacct gtttgccttt ttccacgtct
    18421 gctttgggag atgcggaaac cgcaccggca gccaaaacaa aggccaataa agtcaatcgt
    18481 ctcatggagt gctcctgatt acagcattgg ataacgcaac aatgctcttt tttatattca
    18541 aatatgggat ttttgacccg attaaaaccg atgattctgt aaacgtgtta ttctatacta
    18601 aatttacatt aaattaccac tgtgtttcac ataaaaccaa ccgcatattt ttgctgtcgg
    18661 acaaacggcg gcggaaaaca aggatatgcc catgaacctt tttcaaaacg ccaaattctt
    18721 cacgacggta aaccatctca aagacctgcc ggacacaccg ctcgaaattg cctttgtcgg
    18781 caggagcaat gccggaaaat ccagtgccat caataccctg accaaccatg tccgtcttgc
    18841 ctacgtttca aaaacaccgg gacggacaca gcatatcaac ttcttcgagc tgcagaacgg
    18901 caattttatg gtcgatttgc ccggctacgg ttatgcccaa gtccccgaag cggtacgcgc
    18961 acattgggtc aatctgctcg gcgactacct ccgccatcgg aaacagctta tcgggctggt
    19021 tttgattatg gatgcccgcc atcctttaaa agaactcgac atccgtatgc tggacttttt
    19081 ccacacgacc ggcagaccgg ttcacatcct gctgtcaaaa gccgacaaat tatccaaaaa
    19141 cgaacagata aaaaccctgt cccaagtcaa aaaactgctc aaaccttatt ccgacaggca
    19201 aaacatcagc gtacagctgt tttccagcct gaaaaaacaa ggtattgacg aggccaaccg
    19261 aactgtcgga agctggtttg acgcagcaga tgccgccgct tcctctccag aggaaaactg
    19321 acctcaatta tacggaaacc gtattccccc cacttgaccg gccgcaaaca tttaaaaaat
    19381 tgccactgcc aaatctaaaa tgccgtctga aagacttttc agacggcatt ttgcggagtc
    19441 tttaaaacag ggaatccaac tgctgctgtt tggaatccgt attgctcgga agcaccggcg
    19501 tttcctgtac gtcctgacgc gtttcgtcag accttccctg ctgttcgttt tctactgccg
    19561 cttcatcatc ttcttttgcc cgtcgggaag gttgcggcgc aataccgctg ttgtccagca
    19621 tcaagcccgg atcggttacc atacgttcct tcatatagta ttcgccattg ctgctgacca
    19681 caccttcagg cattttcatc cctttgccct gctttccttt caacgcaaaa cgcatatagt
    19741 ccacccaaac cggcaccgca atcgtaccgc cgtagccggc acgccccata ctcttaggtt
    19801 tgtcgaagcc gatatatacg gcagtaacca catcagggtt aaaaccgaca aaccacgcat
    19861 ctttattgtc gttggtcgta cccgttttac cggcaatatc cgttcttccc aacgcagctg
    19921 ccccccttgc cgtaccgaca cggaccacat cctgcataat cttatacata atataggcat
    19981 tgcgcggatc gattgcctga ggcgcatttt gccctgccac caaaggttgc atttgggcgc
    20041 gcaacctgcc gtctctgtca taaatcttat cgatcacgtg cgaagaaacc ctatatccgc
    20101 cgttcgcaaa tacactatat gcctccgcca ctttcaacgg cgtcgtctcg cccgtaccta
    20161 aagccataga caggcttgcc ggcagctcgg acggcctgaa gccgaaacgc cggatatact
    20221 gttgcgcgta accgacaccg atagacatca aaatacggat ggaaaccata ttcttggaag
    20281 ccgtcagagc ctgtctcaaa gtaatgtagc cggaatatct gccgtctgaa tttttaggtg
    20341 tccaaaccga accgttcggc cctttccccg gcagggaaat cggcgcatcg ttaaccactg
    20401 tggacgcggt catcccctta gataatgccg ccgaatagac aaacggctta aaggtcgaac
    20461 ccggctgccg cattgcctga acggcacgat tgaatgtttt gctgtgaaaa tcataaccgc
    20521 cgaccagcgc gcgcacagct ccggtttttg catccagcga aaccaaagcc ccctgcagca
    20581 acggctcttg aaccaccgcc caacgcccgc cgttgttttt gacacggatg accgcgcccc
    20641 tgcggatacg gtcctccccc attttctcat tatcgaccgc tcgggccgca aaacccaagg
    20701 cgcgcctgtc aagcgcaacc cgcctgccgc cgggcagctg tatgacgaca tttttctttt
    20761 tagtaacatc caacacaacg gcgggaacca ttttatcgac ggtatagagt cccgacagat
    20821 actggctgac agtctcctcg acatcttcac tcttactcaa atcgatatag ttttccgcac
    20881 cgcggtagct gctgccgcga tcgaaattcc gtagagcctt gcgcaatgcc tcggttgccg
    20941 ccttctgatg atcggtgcgg accgtggtat aaaccttaaa accctgcgta taggcatctt
    21001 caccatattt ctcatacagt tcccgacgca ccatttccgc cacatataaa gcactctgat
    21061 cgattttccg aacaaaccgc tcataatgca gttcctcatt caatgcctga tcgcgctgtt
    21121 gcacggtaat catcttctcc tcgagcatat tgttcaaaat atacttctgg cgcaacttgg
    21181 cacgctccgg attaacaatc ggattatagg cagacggagc cttgggcagt cccgcaagca
    21241 tggcggcttc cgccaaagtc aaatctcgga cattcttatt gaaatagatt tgcgcggcag
    21301 atgcaaaacc ataggcgcgc tgaccgaggt aaatctgatt gaaatacaac tcaaggattt
    21361 tgtctttgct taaagactgc tcgattttat aggcaagcaa cacctcattg aatttgcgtg
    21421 tgaacgtttt ttcactgctc aaataaaaat ttttcgccac ctgctgtgta atcgtactcg
    21481 cacccgactg cacgctgccg gacacgacat tgccgacggc agcgcgggca acaccccaaa
    21541 catccacccc ccaatgccgg taaaagcgtt tatcctcggc ggcaataacc gcattccgca
    21601 acacctcggg gaaatcgccg atttttgtaa attcgcgccg ctgctcccca tacataccga
    21661 tgacttctcc atccgccgaa taaatagtca acggcatttt aggctggtaa tgctgcaaag
    21721 aatccaaaga cggcagtttc ggatacgtta ccaaaatagc aatggcaacc agacccactc
    21781 caaatacaca aaaaccaaaa aacaaaccaa aacaagtagt taaaatcttt ttaatcatag
    21841 ctgaataata atttaccatt attggtatta aataaagtaa aatagcaacc gatttctaca
    21901 aagcacggtt tcaatgtgca aagaacaagg aatccattac ggataccgaa acggttactc
    21961 actgtacaaa taaagcagga actttcatca tgcgcttgtt taaaagcttg aaaaacccta
    22021 aaaaaacaga tgccaagctc cctaaaaaat cttcgggact caataaccgc gcggcaatcg
    22081 gcatcgatat cgaccagcat tccatcaaaa tggtacaatt gtcaggacgt agtttaaacc
    22141 aaattcaatt ggaaaaatac gtcattgcca aattaccaaa gaatatcatt caaggcaata
    22201 aagtccaaaa ttacgatcaa cttgttacat atttgcaaca agcctatgcc aaactgggta
    22261 cttcgtgcaa aaacatcgtc gcgtccgtcc cgcaaaatct ggcaaccatc gaacaattga
    22321 cctacacggc caaagatgca gaattggacc tgcaggggtt cgtggagtcc tccatctccg
    22381 aagccagctc gatatcgctc gaagaagcca attacgacta tcaggtcttg tcccaatcgg
    22441 ccgtcggcga agctgttttg tccgtcgcat cgagaaagga tgaaatcgaa cccctgattg
    22501 acgcattcaa cgcagccggt atgaaattat ccgcgcttga tgtggacatt ttcggacaat
    22561 acaacgccta cgcgctgtgg ataaaccatt ttgcccccga gcttgcggac gaaaaagtgg
    22621 ccattttcgg cgtatatgcc gcacagacct acgccctggt catccaagac ggaaaaatcc
    22681 tatacaaaca ggaaacctcc gtcagcgcag aacagctcaa ccaactcatc cagcgcacct
    22741 atcaggtaac agcagaaaaa gcggaagaga tcatcaactc cccgcaaaaa ccttccgatt
    22801 accaagaaag cgtggcaaac tatttcaacc agcagattac ccaagaaata caaagggtct
    22861 tgcagtttta ttacaccacg cagaccgcag acgatatgac cgacatcaag catatcctgc
    22921 tgacggggga agcggtgcgc cagaaaggca tcgcccaaac cgtcgcctcg caaaccaatg
    22981 cggatgtaca atgcgtccat cccgcgcgtt attttgcgaa cgacctcaaa acagacgaac
    23041 aacaattcga acttgatgcg ccgacactga ccaaggcgtt cggtttggcg gtacggggat
    23101 tataattatg aacaatttaa tcaaaatcaa cctcctcccc tacagggaag agatgaacaa
    23161 acgcaaacag cagcagttta aaacgctgat gtacggcgcc gtgctgacgg gcgttgccgc
    23221 cgttgcggca acctacctgt ttatcgacaa tatgatcaat aaacagtcgg aaaaaaacac
    23281 gctgctggaa atctccatcg cacacttgga taccgagctg tcggaaatac aaaagctcaa
    23341 acaggaaaaa gatgccttcc tgattaagaa aaacaaaatc ggggagctcc agatcaaacg
    23401 cctccaagcc gcaaaaatcc tcgacagcct gaatgaggcc gtccccggaa gcacctacct
    23461 aacctcgctg gatgccgtta ccgccgactc ttatcggctc agcggcagga catccagcga
    23521 caaccgcgtt gccgccatga tgagggcgat gcccaatacc ggcatattca agcaacccga
    23581 attgttaagc atcaagaaaa acaattcgca tcaagaattt acccttcagg caacattaca
    23641 acctatcgta aaggcggccg aatccaaaga gaatccggct tcgggaaacg cacaggaggc
    23701 aaactgaatg gcttctaaat catctaaaac caacttggat ctcaacaacc ttcacctgct
    23761 caaccttcct gccaggcttt ttatcgccct gctggtcgtt gccgccgtgc tggggctcgg
    23821 ttatgccgga ttgttcaaaa gccagatgga atcccttgag gaatatgaag caaaagaaac
    23881 cgaactgaaa aacacctaca aacagaaaag tatcgacgcg gccagcctga acaaccttag
    23941 ggacgaactt gcctcaatcc gctctgcctt cgatatcatg ttgaaacagc tgccgacaga
    24001 tgcagaaatt cccaatttgg ttcaagagct tcatcaggcg ggttcgagca acggtctgcg
    24061 cttggacagc gttatgcccc aacctcccgt agatgacggt cccatcaaaa aattacccta
    24121 ttccatttcc attaccggaa attacgaaca gatcagccaa tttacccgcg atgtcggcag
    24181 tctctcccga atcattaccc ttgagtcgct gaaaattgcc caatctccgg aaaacggcgg
    24241 caatcctgac ggcaagagca gtatcctgaa cctcagcgcc attgccacca cctaccaagc
    24301 aaaatccgta gaagagcttg ccgcagaagc ggcacaaaat gccgagcaaa aataacttac
    24361 gttagggaaa ccatgaaaca ctatgcctta ctcatcagct ttctggctct ctccgcgtgt
    24421 tcccaaagtt ctgaagacct aaacgaatgg atggcacaaa cgcgacgcga agccaaagca
    24481 gaaatcatac ctttccaagc acctaccctg ccggttgcgc cggtatacag cccgccgcag
    24541 cttacagggc cgaacgcatt cgacttccgc cgcatggaaa ccgccaaaaa aggggaaaat
    24601 gcccccgaca ccaagcgtat taaagaaacg ctggaaaaat tcagtttgga aaatatgcgt
    24661 tatgtcggca ttttgaagtc cggacagaaa gtctccggct tcatcgaggc tgaaggttat
    24721 gtctacactg tcggtgtcgg caactatttg ggacaaaact acggtagaat cgaaagcatt
    24781 accgacgaca gcatcatcct gaacgagctg atagaagaca gcacgggcaa ctgggtttcc
    24841 cgtaaagcag aactgctgtt gaattcttcc gacaaaaaca ccgaacaagc ggcagcacct
    24901 gtcgcagaac aaaattaaga agaggattac tccattatga ataccaaact gacaaaaatc
    24961 atttccggtc tctttgtcgc aaccgccgcc tttcagacgg catcggcagg aaacattaca
    25021 gacatcaaag tttcctccct gcccaacaaa cagaaaatcg tcaaagtcag ctttgacaaa
    25081 gagattgtca acccgaccgg cttcgtaacc tcctcaccgg cccgcatcgc cttggacttt
    25141 gaacaaaccg gcatttccat ggatcaacag gtactcgaat atgccgatcc tctgttgagc
    25201 aaaatcagtg ccgcacaaaa cagcagccgt gcgcgtctgg ttctgaattt gaacaaaccg
    25261 ggccaataca ataccgaagt acgcgggaac aaagtttgga tattcattaa cgaatcggac
    25321 gataccgtgt ccgcccccgc ccgcccagcc gtaaaagccg cgcctgccgc accggcaaaa
    25381 caacaggctg ccgcaccgtt taccgagtcc gtagtatccg tatccgcacc gttcagcccg
    25441 gcaaaacaac aggcagcggc atcggcaaaa caacaggcgg cgacaccggc aaaacaaacc
    25501 aatatcgatt tccgcaaaga cggcaaaaat gccggcatta tcgaattggc ggcattgggc
    25561 tttgccgggc agcccgacat cagccaacag cacgaccaca tcatcgttac gctgaaaaac
    25621 cataccctgc cgaccgcgct ccaacgcagt ttggatgtgg cagacttcaa aacaccggtt
    25681 caaaaggtta cgctgaaacg cctcaataac gacacccagc tgattatcac aacaaccggc
    25741 aactgggaac tcgtcaacaa atccgccgcg cccggatact ttaccttcca agtcctgccg
    25801 aaaaaacaaa acctcgagtc aggcggcgtg aacaatgcgc ccaaaacctt cacaggccgg
    25861 aaaatctccc ttgacttcca agatgtcgaa atccgcacca tcctgcagat tttggcaaaa
    25921 gaatccggga tgaacattgt tgccagcgac tccgtcaacg gcaaaatgac cctctccctc
    25981 aaagatgtgc cttgggatca ggctttggat ttggttatgc aggcgcgcaa cctcgatatg
    26041 cgccagcaag ggaacatcgt caacatcgcg ccccgcgacg agctgcttgc caaagacaaa
    26101 gccttcttac aagcggaaaa agacattgcc gatctgggcg cgctgtattc ccaaaacttc
    26161 caattgaaat acaaaaatgt ggaagaattc cgcagcatcc tgcgtttgga caatgccgac
    26221 acgaccggaa accgcaacac gcttgtcagc ggcaggggca gcgtgctgat cgatcccgcc
    26281 accaacaccc tgattgttac cgatacccgc agcgtcatcg aaaaattccg caaactgatt
    26341 gacgaattgg acgtacccgc gcaacaagtg atgattgagg cgcgtatcgt cgaagcggca
    26401 gacggcttct cgcgcgattt gggcgttaag ttcggcgcga caggcaggaa aaaactgaaa
    26461 aatgagacga gcgcattcgg ctggggcgtg aactccggct tcgggggcgg caataaatgg
    26521 gaggcccaaa ccaaaatcaa cctgccggtt gccgctgccg caaacagcat ttcgctggtg
    26581 cgcgcgattt cctccggcgc gttgaatttg gaattgtccg catccgagtc gctttcaaaa
    26641 accaaaacgc ttgccaatcc gcgcgtgctg acccaaaacc gcaaagaggc caaaatcgaa
    26701 tccggttacg aaattccttt taccgtaact acagcctcgg gcggcggcaa ctctaccaac
    26761 acggaactca aaaaagccgt cttggggctg accgttacgc cgaacatcac gcccgacgga
    26821 caaatcatca tgaccgtcaa aatcaacaaa gactcgcctg cacaatgtgc ttcaggcagc
    26881 aacacaatcc tatgtatttc gaccaaaagc ctgaatacgc aggctatggt tgaaaacggc
    26941 ggcactttga ttgtcggcgg tatttatgaa gaaaacaacg gcaatacgct gaccaaagtc
    27001 cccctgttgg gcgacatccc cgttatcggc aacctcttta aaacacgcgg gaaaaaaacc
    27061 gaccgccgcg aactgctgat tttcattacc ccgaggatta tagatacggc cggcaacagc
    27121 ctgcgctatt gatgcgtcaa aataagggca tatgttttac agcatatgcc ctttctttat
    27181 gctttttgcc gcgaccgaaa tgccgtcatt cccgcgcagg cgggaatcca acttgtccgg
    27241 tttcggttgt ttttcgtctc gtaacttttg agccgtcatt cccgcgaaat cggaaatcca
    27301 gtccgttcag tttcggtcat ttccgataaa ttcctgttgc ttttcatttc tagattccca
    27361 ctttcgtggg aataacggcg gaagggataa atcctcgcaa tccaaagcct cgtcatttcc
    27421 acaaaaaaca gcaacccgaa acaccccgtc attcccgagc aggcgggaat ctaggtcttt
    27481 aaacttcggt ttttttccga taaattcttg cctcattgaa attctagatt cccgctttcg
    27541 cgggaatgac gggatatggg tttccgtgcg gacgtgttcg gatttccgcc tgcgcgggaa
    27601 tgacgattcg ggtattactg acgattcatc gggccgtagg gtgggcttca gcccactaat
    27661 ttcaccgcat caagggtttt ggggaatagg cgtaactcgg cggtttcggt ttttggcgaa
    27721 gttggttgga atcggcgggc tgaagcacac cctacagtcc accctacaac ccatcttacg
    27781 catccgcctt acgcatttat ccttcgcctg ccgatatgcc gtcattcccg cacaggcggg
    27841 aatctagaac cgcaacgcgg caggaatctg tcggatacgg ctgaa
//
LOCUS       DAWTQS010000021        27712 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.021, whole genome shotgun sequence.
ACCESSION   DAWTQS010000021 DAWTQS010000000
VERSION     DAWTQS010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 27712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27712
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.021"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(1..296)
                     /locus_tag="WNA25_000910"
                     /pseudo
     CDS             complement(1..296)
                     /locus_tag="WNA25_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212793.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(291..391)
                     /locus_tag="WNA25_000911"
                     /pseudo
     CDS             complement(291..391)
                     /locus_tag="WNA25_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(397..651)
                     /locus_tag="WNA25_000912"
                     /pseudo
     CDS             complement(397..651)
                     /locus_tag="WNA25_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212793.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(713..3337)
                     /gene="alaS"
                     /locus_tag="WNA25_000913"
     CDS             complement(713..3337)
                     /gene="alaS"
                     /locus_tag="WNA25_000913"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFA9823697.1"
                     /translation="MKTSELRQKFLKFFETKGHTVVRSSSLVPHDDPTLLFTNAGMNQ
                     FKDVFLGFDKRPYSRATTAQKCVRAGGKHNDLENVGYTARHHTFFEMMGNFSFGDYFK
                     RDAIHFAWEFLTSPEWLNIPKDKLLATVYAEDDEAYNIWLNEIGMPSERIVRIGDNKG
                     AKYASDNFWQMGDTGPCGPCSEIFYDHGKEIWGGIPGSPEEDGDRWIEIWNCVFMQFN
                     RDEQGNMNPLPKPSVDTGMGLERMAAVMQHVHSNYEIDLFQDLLKAVARETGAPFSME
                     EPSLKVIADHIRSCSFLIADGVLPSNEGRGYVLRRIIRRAVRHGYKLGQSKPFFHKLV
                     ADLVQEMGGAYPELKEKQAQIEEALKNEESRFAQTLETGMALLENALAKGGKTLGGEI
                     IFKLYDTYGFPYDLTADICRERNIEPDEAGFEREMEAQRARARAAQSFKANAQLPYDG
                     QDTEFKGYSERQTESKVLALYKDGGQVVELNEGDSGAVVIDFTPFYAESGGQVGDVGY
                     IFAGENRFEVRDTQKIKAAVFGQFGVQTSGRLKVGDSITAKVDDEIRNANMRNHSATH
                     LMHKALRDVLGGHVEQKGSLVTAESTRFDISHPQAVTAEEIAEVERRVNEAILANVAV
                     NAAIMSMEDAQKTDAMMLFGEKYGDEVRVLQMGGFSTELCGGTHVSRTGDIGLFKIIS
                     EGGIAAGVRRIEAITGLNALKWAQEQERLVKDIIAETKAQTEKDVLAKIQAGAAHAKA
                     LEKELARAKAELAVHAGAKLLDDAKDLGAAKLVAAQIEADAAALRETVTDLTGKSDNA
                     VILLAAVNEGKVSLCAGVSKALTGKVKAGDLVKFAAEQVGGKGGGRPDLAQAGGTDTD
                     KLPEMLASAEGWLCQKLS"
     gene            3541..4671
                     /locus_tag="WNA25_000914"
     CDS             3541..4671
                     /locus_tag="WNA25_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFA9823698.1"
                     /translation="MVKHLPLAVLTALLLAACGGSDKPPAEKPAPAENRNVLKIYNWS
                     EYVDPETVADFEKKNGIKVTYDVYDSDETLESKVLTGKSGYDIVAPSNAFVGRQIKAG
                     AYQKIDKSMIPNYKHLNPEMMRLMDGVDPDHEYAVPFYWGTNTFAINTERVKKALGTD
                     KLPDNQWDLVFNPEYTFKLKQCGISYLDSAAEIYPMVLNYLGKNPNSSNTEDIREATA
                     LLKKNRPNIKRFTSSGFIDDLARGDTCVTIGFGGDLNIAKRRAEEAGGKEKIRVMMPK
                     EGVGIWVDSFVIPKDAKNVANAHKYINDFLDPEVSAKNGNFVTYAPSSKPARDLMEDE
                     FKNDNTIFPSGEDLKNSFIMVPIRPAALKFMVRQWQDVKAGK"
     gene            complement(4712..5614)
                     /locus_tag="WNA25_000915"
     CDS             complement(4712..5614)
                     /locus_tag="WNA25_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFA9823699.1"
                     /translation="MDTAKKDILGSGWMLVAAACFTVMNVLIKEASAKFALGSGELVF
                     WRMLFSTVTLGAAAVLRRDTFRTPHWKNHLNRSMVGTGAMLLLFYAVTHLPLTTGVTL
                     SYTSSIFLAVFSFLILKERISVYTQAVLLLGFAGVVLLLNPSFRSGQEPAALAGLAGG
                     AMSGWAYLKVRELSLAGEPGWRVVFYLSATGVAMSSVWATLTGWHTLSFPSAVYLSGI
                     GVSALIAQLSMTRAYKVGDKFTVASLSYMTVVFSALSAAFFLGEELFWQEILGMCIII
                     LSGILSSIRPIAFKQRLQALFRQR"
     gene            5753..6250
                     /locus_tag="WNA25_000916"
     CDS             5753..6250
                     /locus_tag="WNA25_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane lipoprotein lipid attachment
                     site-containing protein"
                     /protein_id="HFA9823700.1"
                     /translation="MAGMKKYLIPLSIAAVLSGCQSIYVPTLTEIPVNPINTVKTEAP
                     AKGFRLAPSHWADVAKISDEATRLGYQVGIGKMTKVQAAQYLNNFRKRLVGRNAVDDS
                     MYEIYLRSAVDSQRGEINTEQSKLYIENALRGWQQRWKNMDAKPDNPAFTNFLMEVMK
                     MQPLK"
     gene            complement(6312..7217)
                     /gene="mtrA"
                     /locus_tag="WNA25_000917"
     CDS             complement(6312..7217)
                     /gene="mtrA"
                     /locus_tag="WNA25_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter MtrCDE transcriptional
                     activator MtrA"
                     /protein_id="HFA9823701.1"
                     /translation="MDILDKLVDLAQLTGSADVQCLLGGQWSVRHETLQCEGLVHIVT
                     AGSGYLCIDGETSPRPVGTGDIVFFPRGLGHVLSHDGKYGESLQPDIRQNGTFMVKQC
                     GNGLDMSLFCARFRYDTHADLMNGLPETVFLNIAHPSLQYVVSMLQLESEKPLTGTVS
                     VVNALPSVLLVLILRAYLEQDKDVELSGVLKGWQDKRLGHLIQKVIDKPEDEWNIDKM
                     VAAANMSRAQLMRRFKSQVGLSPHAFVNHIRLQKGALLLKKTPDSVLEVALSVGFQSE
                     THFGKAFKRQYHVSPGQYRKEGGQK"
     gene            7346..7681
                     /locus_tag="WNA25_000918"
     CDS             7346..7681
                     /locus_tag="WNA25_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFA9823702.1"
                     /translation="MFKDWKEHTALVKKSFGELGKAHPKMLQAYGALEQAAAAEALDA
                     KTRELIAIAVAITTRCESCISVHAAAAAKAGATDSEIAGALATAIALNAGAAYTYALR
                     ALEAVETQK"
     gene            7860..8282
                     /locus_tag="WNA25_000919"
     CDS             7860..8282
                     /locus_tag="WNA25_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823703.1"
                     /translation="MSAVFIAPYLTAFHEQEKIFEYADLTVTAPNRSGRAIKLEADGR
                     QYRLSCYGFDSLCTGGNIGRAIRARQVKIVLSETVGKGFLNGVLLEYRNSGSVYSNKD
                     FSRTEDRLVEVLAQPAVFSLKPGILLLLPAIFLRLKKM"
     gene            8388..8951
                     /gene="pgsA"
                     /locus_tag="WNA25_000920"
     CDS             8388..8951
                     /gene="pgsA"
                     /locus_tag="WNA25_000920"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFA9823704.1"
                     /translation="MPWNIPIFLTWLRVLLIPVLIVLFYLPFSWFSEEAVNVAAAVIF
                     AVAALTDWFDGFLARLWKQTSDFGAFLDPVADKLMVAVSLLLLVKLDRTYVLFAMIII
                     GREITISALREWMAQMGKRSSVAVATVGKFKTAAQMLAIFFLLLNFPDFYGFNLVVIG
                     NILMFIASLLTVWSMLYYLKMAWKEIA"
     gene            9032..9107
                     /locus_tag="WNA25_000921"
     tRNA            9032..9107
                     /locus_tag="WNA25_000921"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9065..9067,aa:Gly,seq:gcc)
     gene            9133..9208
                     /locus_tag="WNA25_000922"
     tRNA            9133..9208
                     /locus_tag="WNA25_000922"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9166..9168,aa:Gly,seq:gcc)
     gene            9234..9309
                     /locus_tag="WNA25_000923"
     tRNA            9234..9309
                     /locus_tag="WNA25_000923"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9267..9269,aa:Gly,seq:gcc)
     gene            9335..9408
                     /locus_tag="WNA25_000924"
     tRNA            9335..9408
                     /locus_tag="WNA25_000924"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9367..9369,aa:Cys,seq:gca)
     gene            9509..9598
                     /locus_tag="WNA25_000925"
     tRNA            9509..9598
                     /locus_tag="WNA25_000925"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9543..9545,aa:Leu,seq:taa)
     gene            9702..10754
                     /gene="sohB"
                     /locus_tag="WNA25_000926"
     CDS             9702..10754
                     /gene="sohB"
                     /locus_tag="WNA25_000926"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFA9823705.1"
                     /translation="MWKEILLNYGIFLLELLTVFGAIALIVLAIVQSKKQSESGSVVL
                     TDFSENYKKQRQSFETFFLSEEETKHQEKKEKKKEKAEAKAEKKRLKEGGEKSAETQK
                     SRLFVLDFDGDLYAHAVESLRHEITAVLLIAKPEDEVLLRLESPGGVVHGYGLAASQL
                     RRLRERNIPLTVAVDKVAASGGYMMACVADKIVSAPFAIIGSVGVVAEVPNIHRLLKK
                     HDIDVDVMTAGEFKRTVTFMGENTEKGKQKFRQELEETHQLFKQFVSENRPGLDIEKI
                     ATGEHWFGRQALALNLIDEISTSDDLLLKAFENKQVIEVKYQEKRSLIQRIGLQAEAS
                     VEKLFAKLVNRRADVM"
     gene            complement(10805..11671)
                     /locus_tag="WNA25_000927"
     CDS             complement(10805..11671)
                     /locus_tag="WNA25_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter six-transmembrane
                     domain-containing protein"
                     /protein_id="HFA9823706.1"
                     /translation="MWKMLKHIAKTHRKRLIGTFSPVGLENLLMLGYPVFGGWAINAV
                     IAGRVWQALLYALVVFLMWLVGAARRIADTRTFTRIYTEIAVPVVLEQRQRQVPHSAV
                     TARVALSREFVSFFEEHLPIAATSVVSIFGACIMLLVLEFWVGVSAVGILALFLWLLP
                     RFAAISENLYFRLNNSLERDNHFIRKGDERQLYRHYGLVSRLRVLISNREAFGYLCVG
                     AAMGILFGFAFVMMTLKGYGSAGHIYSVGTYLWMFAMSLDDVPRLVEQYSNLKDIGQR
                     IEWSERNIKAGT"
     gene            complement(11682..12113)
                     /locus_tag="WNA25_000928"
     CDS             complement(11682..12113)
                     /locus_tag="WNA25_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFA9823707.1"
                     /translation="MNRLDQLGIRINLICNVFDKWIGQQDLNYNLFTVLYTLATEGSR
                     TQKHIGEEWSLPKQTVSGVCKTLAGQGLIEWQEGEQDRRERLLSLTEKGKVHAAPLTE
                     NAQEFSDKVFSTFGDKRTTRLFADLDALAEVMEKTISENKK"
     gene            complement(12348..13217)
                     /locus_tag="WNA25_000929"
     CDS             complement(12348..13217)
                     /locus_tag="WNA25_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFA9823708.1"
                     /translation="MSAETYTQIGWVGLGQMGLPMVTRLLDGGIEVGVYNRSPDKTAP
                     ISAKGAKVYGSTAELVRACPVIFLMVSDYAAVCDILNGVRDGLAGKIIVNMSTISPTE
                     NLAVKALVEAAGGQFAEAPVSGSVGPATNGTLLILFGGSEAVLNPLQKIFSLVGKKTF
                     HFGDVGKGSGAKLVLNSLLGIFGEAYSEAMLMARQFGIDTDTIVEAIGGSAMDSPMFQ
                     TKKSLWANREFPPAFALKHASKDLNLAVKELEQAGNTLPAVETVAASYRKAVEAGYGE
                     QDVSGVYLKLAEH"
     gene            complement(13293..14204)
                     /gene="hisB"
                     /locus_tag="WNA25_000930"
     CDS             complement(13293..14204)
                     /gene="hisB"
                     /locus_tag="WNA25_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HFA9823709.1"
                     /translation="MTKTQLHLNNFLTLAQEAGSLPKLAKLCGYRTPVALYKLKQRLE
                     KQAEDPDARGIRPSLMAKLEKHTGKPKGWLDRKHRERTVPETAAESTGTAETRIAETA
                     SAAGCRSVTVNRNTCETQITVSINLDGSGKSRLDTGVPFLEHMIDQIARHGMIDIDIS
                     CKGDLHIDDHHTAEDIGITLGQAIRQALGDKKGIRRYGHSYVPLDEALSRVVIDLSGR
                     PGLVYNIEFTRALIGRFDVDLFEEFFHGIVNHSMMTLHIDNLSGKNAHHQAETVFKAF
                     GRALRMAVEHDPRMAGQTPSTKGTLTA"
     gene            complement(14221..15300)
                     /locus_tag="WNA25_000931"
     CDS             complement(14221..15300)
                     /locus_tag="WNA25_000931"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFA9823710.1"
                     /translation="MKSVRSFIRNDILAMSAYKITDVPPGFAKLDAMESPAHPFEGHE
                     ALMREWRAQLASAPIHLYPNPSGCGLQEALRSAFDIPDCAAVALGNGSDELIQFITML
                     TAKPGAAMLAAEPGFIMYRHNAALYGMDYVGVPLNGDFTLNLPAVLEAVRKHRPALTF
                     IAYPNNPTGVCFTRAEIEAAIEASDGIVVVDEAYGAFNGDSFLPQAGRIPNLIVLRTL
                     SKIGFAGLRIGYATGCPEVIGELQKILPPYNMNQLSLTTAKLALQHYGIISANIDSLK
                     NERERMFAELGKICRLNAFPSQANFITIRVPDADLLFDTLKQNRILVKKLHGAHPLLE
                     HCLRITIGSSAQNDAVLDVIRRLYR"
     gene            complement(15346..16635)
                     /gene="hisD"
                     /locus_tag="WNA25_000932"
     CDS             complement(15346..16635)
                     /gene="hisD"
                     /locus_tag="WNA25_000932"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFA9823711.1"
                     /translation="MKKLNTQSPDFQAGLKALLAFETAQNPETERIVADICADVQKRG
                     DAALIEYTNKFDQTNAKSIDDLILTQADLNAAFERIPNDVQTALQTAARRVESYHQRQ
                     KMESWSYTDEDGTLLGQQITPLDRVGIYVPGGKAAYPSSVIMNAMPAHVAGVKEIIMV
                     VPTPKGERNDIVLAAAYVAGVTKVFTVGGAQAIAALAYGTETIPQVDKITGPGNAFVA
                     AAKRRVFGVVGIDMVAGPSEILVIADGTTPADWVAMDLFSQAEHDEIAQAILIGTSQA
                     YLDEVEAAMDRLIETMPRRDIIEASLGNRGAMILVKDLNEACEISNYISPEHLELSVE
                     NPQEWAKKIRHAGAIFMGRYTGESLGDYCAGPNHVLPTSRTARFSSPLGTYDFQKRSS
                     LIQVSEQGAQKLGETASVLAHGESLTAHARAAEFRMK"
     gene            complement(16632..17519)
                     /locus_tag="WNA25_000933"
     CDS             complement(16632..17519)
                     /locus_tag="WNA25_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HFA9823712.1"
                     /translation="MVAKIKKFSDSTLSVLNNGERRFYVYCLTDLKKDKILYIGKGCG
                     NRIFEHEWVASRSQDPVSGEIIDRKLKAISKCKKLGRYIISYHLTEVEALAAESALIH
                     FVKSVLGKKLKNKIAGHGPGGISVEELDRRFGFSSLPLSEINPDGLILAIKIHNAFDL
                     DTDEELDYLFDNQDDANLKSRTLGNWVIGKDVASKVKYVIGVHTGLQNAVVSAYEVDG
                     FETMTEETKNGRKQTRYRFRTTSRSEEVLAKLGLQQKCLPELKFGSGGGKAYIRPKTE
                     QETLQTIPSPKITKENPKS"
     gene            complement(17611..18276)
                     /locus_tag="WNA25_000934"
     CDS             complement(17611..18276)
                     /locus_tag="WNA25_000934"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFA9823713.1"
                     /translation="MQDNALTIALSKGRIFEETLPLLAAAGIAPTEEPEKSRKLIIGT
                     NHENIRLVIVRATDVPTYVRYGAADFGIAGKDVLIEHGGTGLYRPLDLEIAKCRMMVA
                     VRKGFDYEAASQPGCRLKIATKYPEIAASHFAGKGVHVDIIKLYGSMELAPLVGLSDA
                     IVDLVSTGNTLKANGLEAVEHIVDISSYLVVNKAALKTKYALLEPIIQSFGGAVKAKW
                     AFI"
     gene            complement(18355..19008)
                     /locus_tag="WNA25_000935"
     CDS             complement(18355..19008)
                     /locus_tag="WNA25_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HFA9823714.1"
                     /translation="MFSVPRSFLPGVFVLAALAACKPQDNSAAQAASSSASAPAAENA
                     AKPQTRGTDMRKEDIGGDFTLTDGEGKPFSLSDLKGKVVILSFGFTHCPDVCPTELLT
                     YSDTLKQLGGQAKDVKVVFVSIDPERDTPEIIGKYAKQFNPDFIGLTATGGQNLPVIK
                     QQYRVVSAKINQKDDSENYLVDHSSGAYLIDKNGEVAIFSPYGSEPETIAADVRTLL"
     gene            19284..19368
                     /locus_tag="WNA25_000936"
     tRNA            19284..19368
                     /locus_tag="WNA25_000936"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19318..19320,aa:Leu,seq:caa)
     gene            complement(19431..19538)
                     /locus_tag="WNA25_000937"
                     /pseudo
     CDS             complement(19431..19538)
                     /locus_tag="WNA25_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            20062..21789
                     /gene="ilvB"
                     /locus_tag="WNA25_000938"
     CDS             20062..21789
                     /gene="ilvB"
                     /locus_tag="WNA25_000938"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HFA9823715.1"
                     /translation="MQLSGAQIIVQSLKAEGVEYVFGYPGGAVIEIYDALFQLNKFKH
                     ILTRHEQAAVHAADAYARVSGKVGVALVTSGPGVTNALTGIATAYTDSIPMVVISGQV
                     GNSLIGTDAFQEVDTVGITRPCVKHNFLVTDINELVETIKKAFQIAASGRPGPVVLDV
                     PKDVTQAMAKFSYPQEDIFIRSYQPVVQGHIGQIKKAVQMLASAKRPVVYFGGGVVLG
                     NASEELTRFVRMTGAPCTGTLMGLGAYPSGDRQFLGMLGMHGTYEANLAMQNADVVLA
                     VGARFDDRVVSVPSKFFEKAKKVIHIDVDPSSIAKRVKADIPIVGDVKNILSEMVALW
                     QKQESVPSEDALGKWWKTIEEWRSRDCLWFDNGSEIIKPQYVIQKLAEITGNSAIITS
                     DVGQHQMFAAQYYPFERPRQWLNSGGLGTMGVGLPYAIGAKLAAPDQDVFCITGDGSI
                     QMNIQELSTCFQYRIPVNVITLNNGYLGMVRQWQEIYYGGRESETYFDSLPDFVKLAE
                     AYGHIGIRVDKKSDVEGALLEALNQKDRLVFIDFLTDQKQNVMPMVGNGKGLDEMVLP
                     PHMRTDGKA"
     gene            21800..22291
                     /gene="ilvN"
                     /locus_tag="WNA25_000939"
     CDS             21800..22291
                     /gene="ilvN"
                     /locus_tag="WNA25_000939"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFA9823716.1"
                     /translation="MRHILSVLIENESGAMSRVVGLFSARDYNIDSLAVAPTEDKTLS
                     RMTIVTHGDEQVIEQITKQLNKLIEVIKVVDLNESRFVERELMLVKVRAAGKDRDEFL
                     RLTEIYRGSIIDVTDRSYTIEITGSTDKLDSFLETVGRAQILETVRTGAAGIGRGERI
                     LKI"
     gene            22358..22651
                     /locus_tag="WNA25_000940"
     CDS             22358..22651
                     /locus_tag="WNA25_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HFA9823717.1"
                     /translation="MSNIKIVALVTVKPEYTETLAAQFKELVKASRAEEGNISYNLHQ
                     EIGKPNRFVFVENWKSQAAIDEHNASAHFQAFVQSVDGKTEALEIVLMNEVAV"
     gene            22731..23744
                     /gene="ilvC"
                     /locus_tag="WNA25_000941"
     CDS             22731..23744
                     /gene="ilvC"
                     /locus_tag="WNA25_000941"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFA9823718.1"
                     /translation="MQVYYDKDADLSLIKGKTVAIIGYGSQGHAHAANLKDSGVNVVI
                     GLRHGSSWKKAEAAGHVVKTVAEATKEADVVMLLLPDETMPAVYHAEVAANLKEGATL
                     AFAHGFNVHYNQIVPRADLDVIMVAPKGPGHTVRSEYKRGGGVPSLIAVYQDNSGKAK
                     DIALSYAAANGGTKGGVIETTFREETETDLFGEQAVLCGGVAELIKAGFETLTEAGYA
                     PEMAYFECLHEMKLIVDLIFEGGIANMNYSISNNAEYGEYVTGPEVVNASSKEAMRNA
                     LKRIQTGEYAKMFIQEGNVNYASMTARRRLNADHQVEKVGARLRAMMPWITANKLVDQ
                     DKN"
     gene            complement(23863..24858)
                     /locus_tag="WNA25_000942"
     CDS             complement(23863..24858)
                     /locus_tag="WNA25_000942"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFA9823719.1"
                     /translation="MNLKNRHFLKLLDFTPEEITTYLDLAAELKDAKKAGREIQRMKG
                     KNIALIFEKTSTRTRCAFEVAARDQGADATYLEPSASQIGHKESIKDTARVLGRMYDA
                     IEYRGFAQETVEELAKYAGVPVFNGLTNEFHPTQMLADALTMREHSGKPLNQTAFAYV
                     GDARYNMGNSLLILGAKLGMDVRIGAPQSLWPSEGIIAAAHAAAKETGAKITLTENAH
                     EAVKGVGFIHTDVWVSMGEPKEVWQERIDLLKDYRVTPELMAASGNPQVKFMHCLPAF
                     HNRETKVGEWIYETFGLNGVEVTEEVFESPAGIVFDQAENRMHTIKAVMVAALGD"
     gene            complement(25000..27585)
                     /gene="acnB"
                     /locus_tag="WNA25_000943"
     CDS             complement(25000..27585)
                     /gene="acnB"
                     /locus_tag="WNA25_000943"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFA9823720.1"
                     /translation="MLEAYRKAAAERAALGIPALPLNAQQTADLVELLKNPPAGEGEF
                     LVELLAHRVPPGVDDAAKVKASFLAAVAEGSASSPLVSPKYATELLGTMLGGYNIHAL
                     IELLDDDKLAPIAAKGLKHTLLMFDSFHDVQEKAEKGNKYAQEVLQSWADAEWFASRA
                     KVPEKITVTVFKVDGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRDGITPDKPGEVG
                     PIKLLEELKAKGHPVAYVGDVVGTGSSRKSATNSVIWHTGEDIPFVPNKRFGGVCLGG
                     KIAPIFFNTQEDSGALPIEVDVSALKMGDVVNILPYEGKIVKNGETVAEFELKSQVLL
                     DEVQAGGRINLIIGRGLTAKAREALKLPASTAFRLPQAPAESKVGFTLAQKMVGRACG
                     LPEGQGVRPGTYCEPRMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHTAAYP
                     KPVDVKTHKELPAFISTRGGVSLRPGDGVIHSWLNRLLLPDTVGTGGDSHTRFPIGIS
                     FPAGSGLVAFAAATGVIPLDMPESVLVRFSGKLQPGVTLRDLVNAIPLYAIKQGLLTV
                     AKAGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTVKLNKEPIIEYMKSN
                     VVLMKNMIANGYQDPRALERRIKAMEKWLANPELLEADKDAEYAAVIEINMDDIKEPI
                     IACPNDPDDVCFMSERSGTKIDEVFIGSCMTNIGHFRAASKLLEGKSDIPVRLWVAPP
                     TKMDAKELSDEGHYGVLGRAGARMEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLG
                     KNTFVYLGSAELAAICSKLGKIPTVEEYQANIGIINEQGDKIYRYMNFNEIDSYNEVA
                     ETVNV"
BASE COUNT         6333 a         6751 c         7570 g         7058 t
ORIGIN      
        1 ttaccgagtt ttaaaaaatt tgctgctttt tctatagcaa cttgccaagt tttatattta
       61 tcgtaatttt tatcaaaacg tactccccct tgtttattgg ataaaaaagt aattatctga
      121 ttcatattaa aaatatttcc attatggaaa actcgtttta aagttaaaaa tttttcagca
      181 ggacataaaa ttatatttgt ttcagcatag attattggtt caccagaaaa ttcttgtgat
      241 gaatgatata tagggctaat aatttttccg cctaaataaa ctccaccagc catatagagt
      301 ggattaaatt taaaccggta cggcgttgcc tcgccttgcc gtactatttg tactgtctgc
      361 ggctcgccgc cttgtcctga tttttgttaa tctactataa aaattaactt tattctctaa
      421 ggttgacaat ttttcaaata ccaaacttgt atcaaggatg ggcaattcta tagttaaacc
      481 tatttctttt gcgagaatat ttaattgttt gtcaattaac catcgacgga caataggagt
      541 tgcaatgcta cgtacatatc ttggaggaag agcatctaca cgaattagtg tttcttttaa
      601 agttgatata tcctcttcta aaagtttttg taactctgtt ttagtattca ttctaacaat
      661 ccaaaatata agccatttga acaagactca aatggcttat taagatttaa atttaactta
      721 atttctgaca aagccaacct tccgcgcttg ccagcatttc gggcaatttg tcggtatccg
      781 taccgccggc ttgcgccaag tcgggacggc cgccgccttt gccgccgact tgttcggctg
      841 caaatttaac cagatctcct gctttgactt ttccggtcag ggctttggat acgccggcgc
      901 acagggagac tttaccctcg tttactgccg ctaaaagaat cacggcgttg tcggattttc
      961 cggtcaggtc ggttacggtt tcgcgcaggg cggctgcgtc ggcttcgatt tgtgcggcaa
     1021 cgagtttggc cgcgcccagg tcttttgcgt cgtccaagag tttggcgcct gcgtggacgg
     1081 cgagttcggc tttggcgcgt gccaattctt tttccaatgc tttggcgtgt gccgcgcctg
     1141 cttggatttt cgccagtacg tctttttcgg tttgggcttt ggtttcggca atgatgtctt
     1201 ttaccaaacg ctcttgctct tgcgcccatt tgagtgcgtt caggccggtg atggcttcga
     1261 tacggcgtac gcctgcggca ataccgcctt cgctgatgat tttgaagagg ccgatgtcgc
     1321 cggtgcgtga aacgtgtgtg ccgccgcaca attcggtaga gaaaccgccc atttgcagta
     1381 cgcgtacttc gtcgccgtat ttttcgccga agagcatcat ggcgtcggtt ttttgcgcgt
     1441 cttccatgct cataatggct gcgttgacgg caacgttggc caagatggct tcgttgactc
     1501 ggcgttctac ttcggcgatt tcttcggcag ttaccgcttg gggatgggaa atgtcgaaac
     1561 gggtggattc ggcggtaacc aaagagcctt tttgttcgac gtgtccgccc aatacgtcgc
     1621 gcagggcttt gtgcatcagg tgggtcgcgc tgtggttgcg catattggca ttgcggattt
     1681 cgtcatccac tttggcggta atgctgtcgc cgactttcag acggcctgaa gtttgtacgc
     1741 cgaattgtcc gaatacggcc gctttgattt tttgggtatc gcgtacttca aagcggtttt
     1801 cgcctgcgaa gatatagccg acgtcgccga cttggccgcc ggattctgca tagaacgggg
     1861 taaagtcgat aacgacggca ccgctgtcgc cttcgttcaa ttcgacgact tgcccgccgt
     1921 ctttgtagag ggcgaggact ttggattcgg tttggcgttc gctataacct ttaaactcgg
     1981 tgtcttgacc gtcgtaaggc agttgggcgt tggctttgaa gctttgggcg gcgcgtgcgc
     2041 gtgcgcgttg ggcttccatt tcgcgctcga agcctgcttc gtccggttcg atattgcgtt
     2101 cgcggcagat gtcggcagtc aggtcgtatg ggaagccgta ggtatcgtag agtttgaaga
     2161 taatttcgcc gccgagtgtt ttgccgcctt tggccagcgc gttttccaac aaagccatac
     2221 cggtttccag agtttgggca aaacggcttt cttcgttttt caaagcttct tcgatttgcg
     2281 cctgtttttc tttcaattca ggatacgcgc cgcccatctc ttgaaccaaa tcggcaacga
     2341 gtttgtggaa gaacggtttg ctttgaccca gtttgtaacc gtggcgcacg gcgcggcgga
     2401 tgatgcggcg caatacgtag ccgcgtcctt cgttggaagg caatacgccg tctgcaatca
     2461 ggaatgagca ggagcggatg tggtcggcga taactttcag gctgggttct tccatactga
     2521 acggcgcgcc ggtttcgcgg gcaacggctt tgagcaggtc ttggaacagg tcgatttcgt
     2581 agttgctgtg gacgtgctgc atcacggcgg ccatacgttc caagcccata ccggtatcga
     2641 cggacggctt gggcagcgga ttcatattgc cttgttcgtc gcggttgaac tgcatgaata
     2701 cgcagttcca aatttcaatc cagcggtcgc cgtcttcttc ggggctgccg ggaatgccgc
     2761 cccagatttc tttgccgtgg tcgtagaaaa tttcggagca ggggccgcaa gggccggtgt
     2821 cgcccatttg ccagaagttg tcggacgcgt atttcgcgcc tttgttgtcg ccgatgcgga
     2881 cgatgcgttc agacggcata ccgatttcgt tcaaccagat gttgtaggct tcgtcgtctt
     2941 ctgcgtaaac ggtcgccaag agtttgtctt tggggatgtt gagccattcg ggggaagtga
     3001 gaaactccca agcgaagtgg atcgcgtcgc gtttgaagta atcgccgaag gagaagttgc
     3061 ccatcatttc aaagaaggtg tggtggcggg cggtgtagcc gacgttttcc aagtcgttgt
     3121 gcttgccgcc tgcgcgtacg catttttgcg cggtggtggc gcggctgtac gggcgtttgt
     3181 cgaaacctaa aaatacgtct ttaaactggt tcatgcctgc gttggtaaac agcagggtcg
     3241 ggtcgtcgtg cggcacgagg ctggaagagc ggacgacggt gtggcctttg gtttcaaaaa
     3301 attttaggaa tttttggcgc agttcggagg ttttcataat tttttcaatg tctctcaaat
     3361 gtcttgtcat ggtaaaagca gggaaaacga acggcggtat attaccgcaa atccctgttt
     3421 ctagctatgg aagcggcggc tttcagacgg cattgcggga ttttgaatgc cgtctgaagc
     3481 cctgttacca atatcggcta taatggccgc tttctccaac ccgatatgca aggaatgata
     3541 atggtcaaac atctgccact cgccgtcctg actgctttgc tgcttgcagc gtgcggcggt
     3601 tcggacaaac cgcctgccga aaaaccggca ccggcggaaa accgaaacgt attgaaaatt
     3661 tataactggt cggaatacgt cgatccggaa accgttgccg attttgaaaa gaaaaacggc
     3721 atcaaggtta cttatgatgt gtacgacagt gatgaaacgc tggaaagcaa ggtgctgacc
     3781 ggaaaatccg gttacgacat tgtcgcgccg tccaatgcgt ttgtgggcag gcagattaag
     3841 gcaggtgcgt atcagaaaat cgataagtcg atgattccca attataaaca tctcaaccct
     3901 gaaatgatga ggctgatgga cggggtcgat cccgaccacg aatacgccgt gccgttttat
     3961 tgggggacaa ataccttcgc catcaatacc gaacgtgtga aaaaggcttt gggtacggac
     4021 aagctgccgg acaaccagtg ggatttggtg ttcaaccccg aatacacgtt caaactcaaa
     4081 caatgcggca tcagctattt ggacagcgcg gcggaaattt atcccatggt gttgaactat
     4141 ttgggcaaaa acccgaacag cagcaatacg gaagacatca gggaggcaac cgccctgctc
     4201 aagaaaaacc gccccaatat caaacgcttt acttcgtccg gctttatcga tgatttggcg
     4261 cgcggcgata cctgcgtaac aatcggtttc ggcggagatt tgaacatcgc caaacgccgt
     4321 gccgaagaag cgggcggcaa ggaaaaaatc cgcgtgatga tgccgaaaga gggcgtgggg
     4381 atttgggtgg attctttcgt gattccgaaa gatgcgaaaa acgtcgccaa cgcgcacaaa
     4441 tacatcaacg acttcctcga tccggaagtg tcggcgaaaa acggcaattt cgttacctac
     4501 gcgccttcga gcaagccggc gcgcgatttg atggaggacg aatttaaaaa cgacaatacg
     4561 attttcccga gcggggaaga tttgaaaaac agctttatca tggtgcctat ccggccggcg
     4621 gcattgaagt ttatggtgcg ccagtggcag gatgtgaagg cggggaaata aagcccgata
     4681 tgccgtctga aggatgttcg gacggcattt tttatctttg gcggaagagg gcttgcagcc
     4741 gctgtttgaa ggcaatgggg cggatgctgc tcaaaatgcc gctgaggata atgatgcaca
     4801 tgccgagtat ttcctgccag aaaagctctt cgcccagaaa aaatgcggca gacagggcgg
     4861 aaaagacgac ggtcatatag gaaagcgagg caaccgtgaa tttgtcgccg actttgtagg
     4921 cgcgcgtcat cgacagttgg gcaatcagcg cggacacgcc gatgcccgac agataaaccg
     4981 ccgatggaaa ggacagggtg tgccagccgg tcagcgtcgc ccaaaccgac gacatcgcca
     5041 cgccggttgc ggaaaggtaa aacacgacgc gccagccggg ttcgcccgcc aaagacagtt
     5101 cgcgcacttt caaatacgcc cagccggaca tcgcgccgcc cgccagcccg gcgagtgccg
     5161 ccggttcctg accgctgcgg aacgagggat taagcagcaa taccacgccg gcaaaaccaa
     5221 ggagcagcac cgcctgcgtg taaacggaaa tccgttcttt caaaatcagg aaggaaaata
     5281 ccgccaaaaa aatcgacgag gtgtaactca gggtaacgcc ggttgtcaaa ggcagatgcg
     5341 ttaccgcgta aaacagcagc agcatcgccc ccgtcccgac catactgcgg tttaagtggt
     5401 ttttccaatg gggcgtgcgg aaggtgtcgc gccgcaatac ggcggcagca ccgagcgtaa
     5461 cggttgaaaa cagcatgcgc caaaagacca attcgccgct gccgagggca aattttgccg
     5521 atgcctcttt aatcaatacg ttcataacgg tgaagcaggc cgccgccacc agcatccagc
     5581 ccgatcctaa aatgtctttt tttgcggtat ccataaacgg tcgttgcgat aaggacggtc
     5641 ggattgtaaa ccttgcggca aggcttgtgg aatgtgtttt tgcctgcttc tgatgccgaa
     5701 attttatttt tcttgccgaa caatttgttt tctcaaggca aacttgatta taatggcggg
     5761 catgaaaaaa taccttatcc ctctttccat tgcggcagtc ctttccggct gccagtctat
     5821 ttatgtgccc acattgacgg aaatccccgt gaatcctatc aataccgtca aaacggaagc
     5881 acctgcaaaa ggttttcgcc tcgccccttc gcattgggcg gatgttgcca aaatcagcga
     5941 tgaagcgacg cgcttgggct atcaggtggg tatcggtaaa atgaccaagg ttcaggcggc
     6001 gcaatatctg aacaacttca gaaaacgcct ggtcggacgc aatgccgtcg atgacagtat
     6061 gtatgaaatc tacctgcgtt cggcggtaga cagccagcgc ggcgaaatca atacggaaca
     6121 gtccaagctg tatatcgaga atgccttgcg cggctggcag cagcgttgga aaaatatgga
     6181 tgccaaaccc gataatcccg catttaccaa ctttttgatg gaagtgatga agatgcagcc
     6241 cttgaaatga cgcggtacgc aaatgccgtc tgaaagcttt ttcagacggc atttgcgttt
     6301 gaagccccga tttatttttg cccgccttct ttccggtatt gccccggcga aacgtgatat
     6361 tgccgtttga acgccttgcc gaaatgcgtt tccgactgaa agcccaccga cagcgcgacc
     6421 tccaaaaccg aatccggggt tttcttcagc agcaatgcgc ctttttgcag gcggatatgg
     6481 ttcacaaagg cgtgcgggct gagtccgact tggcttttga agcggcgcat cagttgcgcg
     6541 cgcgacatat tggcggcggc aaccattttg tcaatattcc attcgtcttc cggtttgtct
     6601 atcacctttt ggatcaaatg tcccaaacgt ttgtcctgcc aacctttcaa tacgcccgag
     6661 agttcgacat ccttatcctg ttcgagatag gcgcgcagga taagcaccag caggacggac
     6721 ggtaatgcgt tgaccacgga aaccgtcccc gtcaaaggtt tttcgctttc cagttgcagc
     6781 attgaaacca catactgcaa acttggatgg gcaatgttca gaaaaacggt ttccggcagc
     6841 ccgttcatca aatcggcgtg ggtgtcgtag cggaaacggg cgcaaaacag gctcatatcc
     6901 agcccgttgc cgcactgttt gaccataaat gtgccgtttt gccgtatgtc cggttgtaaa
     6961 ctttctccgt attttccgtc gtggctcaac acatgaccca agccgcgcgg gaaaaataca
     7021 atatcgcccg tgccgaccgg acgcggggaa gtttcgccgt cgatgcagag ataaccgctg
     7081 cccgccgtaa caatgtgtac cagcccttcg cattgcaagg tttcatgccg taccgaccat
     7141 tgtccgccca aaaggcactg cacatccgca ctgcccgtca attgggcgag atcgaccagt
     7201 ttgtccagaa tgtccataaa tctttttgaa ccataaaatg agatgattaa acgagaaaca
     7261 caactgaata attgcaataa tacgcacatc aaaaacagat acgcaagcgc gtatcagggt
     7321 taaacgcaag agaaaggaaa agaaaatgtt taaagattgg aaagaacata ccgcattggt
     7381 taaaaaatca ttcggcgagc tgggtaaggc gcatcctaaa atgctgcaag cctacggcgc
     7441 attggaacag gccgccgccg ccgaagcact cgatgccaaa acgcgcgaac tgattgccat
     7501 cgccgttgcc atcaccaccc gttgcgagag ctgcatcagc gttcacgccg ccgccgccgc
     7561 caaagccggt gcgaccgaca gcgagatcgc aggtgcattg gcaaccgcca ttgccctgaa
     7621 tgcgggtgct gcttacactt acgccctgcg tgcattggaa gcggttgaaa cgcaaaaata
     7681 atccgtttcg gataagaaat gccgtctgaa aatatttcag acggcatttt gcctgttatg
     7741 cttcccgata ttttgcagac aacatatgca gccgtttttt tcagacggca tttgagaaac
     7801 atggaacaag tatgaaatat caccgcttgg cattgtttgc cgccataagc tgcctgttgt
     7861 tgtccgccgt ttttattgca ccttacctga cggcatttca cgaacaagaa aaaatatttg
     7921 aatatgcgga tttgaccgtt accgccccca accgcagcgg acgggcaatc aaattggagg
     7981 cggacgggcg gcagtaccgg ctttcctgct acgggttcga cagtttgtgc acaggcggca
     8041 atatcggcag agccatcagg gcgcggcagg ttaagattgt tttaagcgaa accgtcggca
     8101 aaggtttttt aaacggcgtt ctgttggaat accgcaacag tggcagtgtc tatagcaata
     8161 aagatttttc ccgcacggaa gaccgccttg tcgaagtgtt ggcacaacct gccgttttca
     8221 gcctgaaacc gggtattttg cttttattgc ccgccatttt cctgcgtttg aaaaaaatgt
     8281 gaaaacagat tggcggggcg ggggaatgac tatgcggcaa ttttacgttt tgggttatga
     8341 ggataaccgt tataatcata attttacagt ccgcacagag aaaaccgatg ccttggaaca
     8401 tccccatctt cctcacatgg ttgagggtct tgctcattcc cgtactgatt gtccttttct
     8461 acctgccgtt ctcatggttt tcagaagaag cagtcaatgt tgccgccgcc gtcatttttg
     8521 ccgttgccgc cttgaccgac tggtttgacg gatttttggc aaggttgtgg aaacagactt
     8581 ccgatttcgg cgcattcctc gaccccgtcg ccgacaagct gatggttgcc gtatcgctgc
     8641 tgctgctggt caaactcgac cggacctatg ttttgttcgc gatgattatc atcggcaggg
     8701 aaattaccat ttccgcattg cgcgaatgga tggcgcaaat gggaaaaagg agcagcgttg
     8761 ccgtcgccac cgtcggcaag ttcaaaaccg ccgcgcaaat gctggcgatt ttctttttgc
     8821 tgctgaattt tcccgatttt tacggattta atcttgttgt tatcggcaac atattgatgt
     8881 ttatcgcatc tttgctgaca gtctggtcga tgctgtatta tctgaaaatg gcgtggaaag
     8941 aaattgcctg aaaaaaacat aaaaatagct tgacggtaaa aacataatcc ataataattg
     9001 cgtcttcttc gatgtcggag agtgaaatcg ggcgggaata gctcagttgg tagagcgcaa
     9061 ccttgccaag gttgaggtcg cgagttcgag actcgtttcc cgctccaaaa atttcattct
     9121 tccgtaatca ttgcgggaat agctcagttg gtagagcgca accttgccaa ggttgaggtc
     9181 gcgagttcga ggctcgtttc ccgctccaaa aatttcattc ttccgtaatc attgcgggaa
     9241 tagctcagtt ggtagagcgc aaccttgcca aggttgaggt cgcgagttcg agactcgttt
     9301 cccgctccaa gttttgtaga caagccagtt ttagggcgag atagcaaagt ggttatgcag
     9361 cggattgcaa atccgtctac gccggttcga ttccgactct cgcctccata tatcttacgg
     9421 cggggtggca gagtgtttat gctatgaggg gtgcaacctt catataggcc ggttaaaatc
     9481 cgcgcccccg cctccacctt tcacaaatgc ccgggtggtg aaataggtag acacaacgga
     9541 cttaaaatcc gtcgggacta aacatcccat gccggttcga ttccggctcc gggcaccaag
     9601 ctgaaaatga caatgccgct ccaagcggtt atttttttat ctgtcggatg gggatggaca
     9661 atattgatat gttcatgcct gaacaagagg aaatccaatc aatgtggaaa gaaattttac
     9721 tgaattacgg tattttcctg ctcgaactgc ttaccgtgtt cggcgcaatt gcgctgattg
     9781 tgttggctat cgtacagagt aagaaacagt cggaaagcgg cagtgtcgta ctgacagatt
     9841 tttcggaaaa ttataaaaaa cagcggcaat cgtttgaaac attcttttta agcgaggaag
     9901 agacaaaaca tcaggaaaaa aaagaaaaga aaaaggaaaa ggcggaagcc aaagcagaga
     9961 aaaagcgttt gaaggagggc ggggagaaat ctgccgaaac gcaaaaatcc cgcctttttg
    10021 tgttggattt tgacggcgat ttgtatgcac acgccgtaga atccttgcgt catgagatta
    10081 cggcggtgct tttgattgcc aagcctgaag atgaggttct gctcagattg gaaagtccgg
    10141 gcggcgtggt tcacggttac ggtttggcgg cttcgcagct taggcgtttg cgcgaacgca
    10201 atattccgct gaccgtcgcc gtcgataagg tcgcggcaag cggcggctat atgatggcgt
    10261 gtgtggcgga taaaattgtt tccgctccgt ttgcgattat cggttcggtg ggtgtggtgg
    10321 cggaagtgcc gaatatccac cgcctgttga aaaaacatga tattgatgtg gatgtgatga
    10381 cggcgggcga atttaagcgc acggttactt ttatgggtga aaatacggaa aagggcaaac
    10441 agaaattccg gcaggaactg gaggaaacgc atcagttgtt caagcagttt gtcagtgaaa
    10501 accgccccgg gttggatatt gaaaaaatag cgacgggcga gcattggttc ggccggcagg
    10561 cgttggcgtt gaacttgatt gacgagattt cgaccagtga tgatttgttg ttgaaagcgt
    10621 ttgaaaacaa acaggttatc gaagtgaaat atcaggagaa gcgaagcctg atccagcgca
    10681 ttggtttgca ggcggaagct tccgttgaaa agttgtttgc caaacttgtc aaccggcgag
    10741 cggatgtgat gtagtttgcc gaacggatgg aaatgccgtc tgaagtgtgt tcagacggca
    10801 tttttcaagt tccggctttg atgttccgtt ccgaccactc tatccgttgt ccgatgtctt
    10861 tcaaattgga atattgttcg accaatcgcg gcacatcgtc caaactcatg gcaaacatcc
    10921 acagataagt gccgaccgaa taaatatgcc ccgcgctgcc gtagcctttg agcgtcatca
    10981 tcacaaaagc aaagccgaac aaaataccca tcgccgcgcc gacgcagaga tagccgaagg
    11041 cttcgcggtt ggaaatcagc acacgcaggc gcgaaaccag tccgtaatgg cggtacagct
    11101 gccgctcgtc gccttttcgg ataaagtggt tgtcgcgttc caagctgttg ttcaggcgga
    11161 aatacaggtt ttcgctgatg gcggcaaaac gtggcaaaag ccataaaaac aacgcaagta
    11221 tgcccaccgc cgacacgccg acccaaaatt ccagcaccag cagcatgatg cacgcgccga
    11281 atatggatac gacggatgtc gcggcaatcg gcaggtgttc ttcaaaaaag ctgacaaatt
    11341 cacgcgacag ggcaacccgt gcagttaccg ctgaatgcgg gacttgccgc tgccgttgtt
    11401 ccaacacaac cggcacggcg atttcggtat aaatccgcgt aaacgtgcgc gtatcggcaa
    11461 tccgccgtgc cgcaccgacc agccacatca aaaatacaac caaagcgtac agcaacgcct
    11521 gccacaccct ccccgcaatc acggcattaa tcgcccagcc gccaaacacc ggatacccca
    11581 gcatcaaaag gttttccagt ccgaccgggg aaaatgtgcc aatcaatcgc ttgcggtggg
    11641 ttttggctat gtgtttcaac attttccaca tatttgtccc tctatttttt attttccgag
    11701 attgtttttt ccatcacttc cgccagtgca tccaaatcgg caaacagccg ggttgtgcgc
    11761 ttgtcgccga atgtggaaaa tactttgtcg ctgaattcct gcgcgttttc tgttaaaggt
    11821 gcggcatgga ctttgccttt ttcggtcagc gacagcaacc gttcgcgcct gtcctgttcg
    11881 ccttcctgcc attcaatcaa cccttgtccg gcaagggttt tgcatacgcc tgaaacggtc
    11941 tgtttgggca gactccactc ttctccgatg tgcttttgcg tgcggctgcc ttcggttgcc
    12001 agggtataaa gtacggtaaa gaggttgtaa ttcaaatcct gctgcccgat ccatttgtca
    12061 aagacattgc aaatcaggtt gatacggatt ccaagttggt cgagtcggtt cataattggt
    12121 cttgatattg actaaatttg tgcggattat agtggattaa caaaaaccgg tacggcgttg
    12181 cctcgccttg tcgtactatt tgtactgtct gcggcttcgt cgccttgtcc tgatttttgt
    12241 taatccacta tatagtcata atcgggactg atttcaagcg tggggcgata aaaatgccgt
    12301 ctgaaacgct ttctggcttc agacggcata ttgtttggaa agggaagtca gtgttctgcc
    12361 aatttcaggt aaacgccgga aacgtcctgt tcgccgtagc cggcttcaac tgctttgcgg
    12421 tagctggcag caacggtttc gacggcgggc agggtgttgc ctgcctgttc aagctctttg
    12481 acggcgaggt taaggtcttt ggaagcgtgt ttgagtgcaa aggcaggggg gaactcacgg
    12541 tttgcccata gtgatttttt tgtttgaaac ataggcgagt ccattgccga gccgccgatg
    12601 gcttcgacga tggtgtcggt atcgatgccg aactgccgcg ccatcagcat cgcttcgctg
    12661 tacgcttcgc cgaaaatgcc taagagcgag ttcaagacga gtttcgcgcc cgagcctttg
    12721 ccgacatcgc cgaaatggaa ggtttttttg ccgacaaggg aaaatatttt ttgcagcggg
    12781 tttaaaacgg cttcgctgcc gccgaacaga atcagcagtg tgccgttggt ggcgggtccg
    12841 accgatccgg aaacgggtgc ttcggcaaac tgtccgcctg cggcttcgac aagtgctttg
    12901 acggcgaggt tttcggtcgg ggagatggtg ctcatgttga cgatgatttt gccggccaat
    12961 ccgtcgcgga ctccgttcag gatgtcgcac acggcggcat agtcggaaac catcaggaaa
    13021 atgacgggac aggcgcggac gagttcggcg gtgctgccgt aaacttttgc tcctttggcg
    13081 gagatggggg cagttttgtc gggcgagcgg ttgtatacgc cgacttcgat gccgccgtcc
    13141 aagagccgcg ttaccatagg cagacccatt tgccctaagc ctacccagcc gatttgtgtg
    13201 tatgtttctg cggacatagt gtttctcctt tgttggggcg ctgtaatgcc gtctgaaggc
    13261 ttcctgcgct tcagacggtc tgtttgggga tgttatgcgg tcagcgtgcc tttggtcgag
    13321 ggggtttgcc cagccattct cgggtcgtgt tcgactgcca tacgcagggc gcgcccgaag
    13381 gctttgaata cggtttccgc ctgatggtgg gcgtttttgc cgctgaggtt gtcgatgtgc
    13441 agggtcatca tgctgtggtt gacgatgccg tggaaaaatt cttcaaacaa atcgacatcg
    13501 aaacgtccga ttagtgcgcg ggtaaattcg atgttgtaca cgagtccggg gcggccggaa
    13561 aggtcgatga cgacgcggct gagggcttcg tcgagcggga cgtaggaatg tccgtaacgg
    13621 cggatgcctt ttttgtcgcc gagtgcctgc cggattgctt gtccgagtgt gatgccgatg
    13681 tcttcggcgg tgtggtggtc gtcgatgtgc aggtcgcctt tgcagctgat gtcgatgtca
    13741 atcatgccgt ggcgggcgat ttggtcgatc atgtgttcga ggaagggaac gccggtatcc
    13801 agcctgcttt tgccgctgcc gtcgaggttg atggagacgg tgatttgggt ttcgcaggta
    13861 ttgcggttga cggtaacgct gcggcagccg gcagcagatg cggtttcggc aattcgggtt
    13921 tcggcagttc cggtgctttc tgcggcggtt tcggggacag tgcgttcgcg gtgttttctg
    13981 tcgagccagc ctttgggttt gccggtgtgt ttttcgagtt ttgccatcag gctgggacgg
    14041 atgccgcgcg catcggggtc ttctgcctgc ttttcaaggc gttgtttgag tttgtaaagt
    14101 gcgacggggg tacggtagcc gcagagtttg gcaagcttgg gcagcgaacc tgcttcttgg
    14161 gcgagagtca ggaagttgtt caggtggagt tgtgtcttgg tcatagggat tccgtttggt
    14221 ttatcggtag agccggcgga taacgtcgag gacggcatcg ttttgtgcgg agctgcctat
    14281 ggtaatgcgc aggcagtgtt ccaaaagcgg atgtgcgccg tgcagttttt tgacgaggat
    14341 gcggttttgt ttgagtgtgt caaacaacaa atcggcatcg ggtacgcgta tggtaatgaa
    14401 gtttgcctga cttggaaagg cgttcagacg gcatattttg cccaattcgg cgaacatccg
    14461 ttcgcgttcg tttttcaggc tgtcgatgtt ggcagaaatg atgccgtagt gttgcagggc
    14521 gagtttggcg gtggtcaggc tcagttggtt catattgtag ggcggcagga ttttttgcag
    14581 ttcgccgatg acttcgggac agcctgtcgc ataaccgata cgcagtccgg caaaaccgat
    14641 tttgctgagg gtgcgtaaga ctatcaggtt gggaatcctg cccgcctgcg gcaggaagct
    14701 gtcgccgttg aatgcgccgt aggcttcgtc gacgacgacg atgccgtctg aagcttcgat
    14761 ggcggcttcg atttcggcac gcgtgaaaca tacgccggtc gggttgttcg ggtaggcgat
    14821 aaaggtcagg gcagggcggt gcttcctgac ggcttcgagg acggcgggca ggttgagggt
    14881 gaaatctccg ttcagtggaa cgccgacata atccatgccg tacagcgcgg cgttgtggcg
    14941 gtacatgatg aaaccgggtt cggctgccaa cattgccgcg ccgggtttgg cggtcagcat
    15001 ggtgatgaac tgtatcagtt cgtccgaacc gttgcctaag gcgacggcgg cgcagtcagg
    15061 gatgtcgaac gccgaacgca atgcttcctg caaaccgcag ccggaggggt tgggatagag
    15121 gtggatgggg gcggatgcca gttgcgcccg ccattcccgc atcagggctt cgtgcccctc
    15181 aaaaggatgg gcggggcttt ccatcgcatc gagtttggca aaaccgggcg gaacatcggt
    15241 gattttgtat gcggacatgg cgaggatgtc gttgcggatg aaggagcgga cggatttcat
    15301 cgtgtttcct taatggttgg aatatgcctg tacgccgttt cggcattatt tcatacggaa
    15361 ctctgccgcg cgtgcgtggg cggtcaggct ttcgccgtgt gccagcacgc tggcggtttc
    15421 gcctaatttt tgcgcgccct gttccgaaac ctgaatcagg ctggagcgtt tttggaaatc
    15481 atatgtcccc aaaggcgagg aaaagcgggc ggttcggctg gtgggcaaca catggttcgg
    15541 gccggcgcag tagtcgccga ggctttcgcc ggtgtagcgt cccatgaaaa tcgcgccggc
    15601 atggcggatt tttttcgccc attcctgcgg gttttcgact gacagttcca agtgttcggg
    15661 ggaaatgtag ttggagattt cgcaggcttc gttcaagtct ttaacgagta tcatcgcgcc
    15721 cctgttgccg agcgaggcct cgatgatgtc gcggcgcggc atggtttcga tgagcctgtc
    15781 catggcggct tctacttcgt cgagatacgc ttgcgacgtg ccgatgagga tggcttgggc
    15841 aatttcgtcg tgttcggcct ggctgaacaa atccatcgcc acccaatcgg cgggtgtcgt
    15901 gccgtcggcg atgaccagta tttcagacgg ccccgccacc atgtcgatgc cgaccacgcc
    15961 gaacacgcgg cgtttggcgg cggcgacgaa ggcgttgccc ggaccggtga ttttatcgac
    16021 ttgggggatg gtttccgtgc cgtaggcgag ggcggcgatg gcttgcgcgc cgccgacggt
    16081 gaagactttg gttacgccgg cgacgtatgc ggcggcaagc acgatgtcgt tgcgttcgcc
    16141 tttcggtgtc ggtacgacca taatgatttc tttcacgcct gcgacgtggg cgggcatcgc
    16201 gttcatgatg acggaactcg gatatgccgc cttgccgcct gggacgtaaa tgccgacgcg
    16261 gtcaagcggg gtaatctgtt gccccagcag cgtgccgtct tcatcggtgt agctccacga
    16321 ttccattttt tggcgttggt ggtagctttc gacacggcgg gcggcggtct gcaatgctgt
    16381 ctgaacgtcg ttcggaatgc gctcgaacgc cgcgttcaaa tcggcttgcg tgagtattaa
    16441 atcatcgatg cttttagcgt ttgtctgatc gaatttgttg gtgtattcaa tcaaagccgc
    16501 atcgccgcgc ttttgcacgt cggcgcaaat gtcggcgacg atgcgttcgg tttcggggtt
    16561 ttgcgcggtt tcaaaagcca gcagggcttt gagtccggct tggaaatcgg gcgattgggt
    16621 gttgagtttt ttcatgattt gggattctcc tttgttattt tgggacttgg gatcgtctga
    16681 agcgtctctt gctcggtttt gggtctgata tacgcttttc ctcccccgct accaaacttc
    16741 aattcgggca ggcatttttg ttgcagaccg agtttggcta atacctcttc gctacgagag
    16801 gtagtacgga aacggtaacg ggtttgtttt ctgccgtttt tggtttcctc agtcattgtt
    16861 tcaaaaccgt ccacttcgta cgcactgaca acagcgtttt gcagaccggt gtgaacgccg
    16921 ataacgtatt tcacttttga agcaacatct ttaccgataa cccagttgcc caacgtacgc
    16981 gatttgaggt tggcatcgtc ttggttgtcg aaaaggtagt ctaattcttc gtcagtatct
    17041 aaatcgaaag cattgtggat cttgatggcg agaatcagcc cgtcggggtt aatctcgcta
    17101 agtgggagag aagagaatcc aaagcggcga tctagttctt ctacgctaat accacccgga
    17161 ccatgcccgg caattttatt tttgagtttt ttacccaaga cagatttaac aaaatgaatt
    17221 aaggcagatt cggcggcgag cgcttcgact tcagtcagat gatagctgat gatatagcga
    17281 ccgagtttct tgcatttgga gatggctttg agtttccgat cgataatctc gccggagact
    17341 gggtcttgtg aacgactagc aacccattca tgctcgaaga tacgattacc gcaacctttt
    17401 ccgatgtaga ggattttgtc ttttttcagg tcggtcagac aatagacata aaaccgacgc
    17461 tcgccgttat tcaaaacgga aagggtcgaa tctgagaatt tttttatttt tgcaaccatt
    17521 ttaacttctc ctatacagtt tactgccggt gatattttca gacggcctgt cggcgggaac
    17581 ggatagggtc gtctgaaaac gcatctttat tcaaatgaat gcccacttcg ccttcactgc
    17641 gccgccgaac gactgaataa tcggctccag cagcgcgtat ttcgttttca aagcagcctt
    17701 gttgaccacc aggtagctgg aaatgtcgac gatgtgttcg actgcctcca agccgtttgc
    17761 cttcaaggtg tttcccgtcg aaaccaagtc cacaatcgca tcgctcaagc cgaccagcgg
    17821 cgcaagttcc atcgagccgt acagtttgat aatgtccaca tggacaccct tgccggcaaa
    17881 atgagatgcc gcgatttcag gatacttcgt ggcaatcttc agacggcatc cgggttgcga
    17941 agcggcttcg taatcaaacc ctttacgcac agcaaccatc atgcggcact tggcaatctc
    18001 caaatccaaa ggtcggtaaa gccccgtgcc gccgtgttcg atcagcacgt ctttgcccgc
    18061 aatgccgaag tccgccgcgc cgtagcggac ataagtcggc acatcggttg cgcggacaat
    18121 gacaaggcgg atgttttcat ggttcgtccc gataatcagc ttgcgcgatt tttcgggctc
    18181 ttcagtcgga gcaatgccgg cagcggcaag cagcggcagc gtctcctcaa aaatgcgccc
    18241 tttggataag gcgatggtca aagcattatc ctgcatggtg tggatgtcct ttgtcggttg
    18301 aaacaataat gccgtctgaa caggcgccgg cggtgcagac ggcatacggt tttatcagag
    18361 cagggtcctt acatcggcag caatcgtttc cggctcgctt ccgtaaggcg agaaaatggc
    18421 aacctcaccg tttttatcga taagatacgc accggaagag tggtcgacca aatagttttc
    18481 gctgtcgtct ttttgattga ttttggcaga aaccacgcgg tattgctgct tgatgaccgg
    18541 caggttttga ccgcccgttg ccgtcagacc gataaagtcc ggattgaact gtttggcata
    18601 cttgccgatg atttcaggcg tgtcgcgttc cggatcgatg ctgacgaaca ccactttcac
    18661 gtccttagcc tgcccgccca actgcttcaa agtgtcgctg tacgtcaaaa gctctgtcgg
    18721 gcagacatcg ggacagtgcg taaagccgaa agacagaatc acgaccttgc ctttcaaatc
    18781 gctcaggctg aaaggcttgc cttcgccgtc ggtcagcgtg aaatcgccgc cgatgtcttc
    18841 cttacgcata tccgtaccgc gcgtttgcgg ctttgccgca ttttccgcag ccggcgcgga
    18901 tgcacttgaa gaagcggctt gcgccgcact gttgtcttga ggtttgcagg cggcaagcgc
    18961 ggcaagtacg aaaacgcccg gcaaaaagga acgcggtacg gaaaacatga caatctccca
    19021 agaataaata gaaaaacctg attcgtacgg aatttgaata tttccatctt aacgcgtttg
    19081 gcagatttaa tgtagtattt ttttacgttg tccgtacggc aggaattgcc gaatcataca
    19141 gaatctttgg agcggaaaga acagtaagat taaaataata aaaattctat ttaaaatcaa
    19201 aaacaaaata aaaacagata aaataatttc aaaaaaaggt ttacagaatc tcaaaatcca
    19261 ttataatggc aatttcttca ccagcccagg tggcgaaatt ggtagacgca ggggactcaa
    19321 aatcccccgc cgcaaggtgt gtcggttcga gtccgaccct gggcaccaca accgcttttg
    19381 gagcggttat ttttttgtct attgctgttt aatgaaatag ataagactaa atatagtgga
    19441 ttaaatttaa accggtacgg cgttgcctcg ccttgccgta ccatctgtac tgtctgcggc
    19501 ttcgtcgttt tgtcctgatt taaatttaat ccactataca atcaatatat ttaagtcgtt
    19561 gttagccctc ttggttctgt caatgccgtc cggtgcaatg agccattttc aggaagtatg
    19621 gttgggaata tttttgatat atcaaaaata aaatcaagtt caatcaatat cccgagtgaa
    19681 accctgtaaa ttttaaaatt cttgatactt tcacatctgc ccccatgcaa actgtcaaca
    19741 atttccatct ttcaaaaaag gtactcctga ttttattctt ttaaaatcaa tcaataattt
    19801 ctccgctttc aaaaaaattt ctccgctttc aaaaaatatt aaaagatgaa agatttttct
    19861 tgattgaact gtcggtttgc gttatcgttt ttactctttt tcacaaactc tgtgttcctt
    19921 tccaattgat tggataatct gtctgttgcc gtgttccccc aatgcgcgta ccctaaatcg
    19981 ctgcttggtg gaattgcatt acaggtgctg tggcaaagcg gtttgtccct attggtttga
    20041 aaccgtataa aagaggtcgt tatgcagtta tcaggcgcgc aaatcatagt gcagagtctc
    20101 aaagccgaag gcgtcgagta cgttttcggt tatcccggcg gtgcggttat cgaaatctac
    20161 gatgccctgt tccaactcaa taaattcaaa cacattctta cccgccatga gcaggcggca
    20221 gtacacgcgg cagatgcgta tgcgcgcgtc agcggtaagg tgggcgtggc attggttaca
    20281 tccggcccgg gcgttaccaa tgcgctgacc ggtattgcta ctgcctatac ggattcgatt
    20341 ccgatggtgg tcatcagcgg acaggtaggc aattccctga ttggtacgga tgcgttccaa
    20401 gaagttgata cggtgggtat tacccgtccg tgcgtcaaac acaatttctt ggttacggac
    20461 atcaatgagt tggtggaaac cattaaaaag gcgttccaaa ttgccgcaag cggccgaccg
    20521 gggcccgtgg tgcttgatgt cccgaaagat gttacgcagg cgatggcgaa attcagctat
    20581 cctcaggaag acatttttat ccgttcgtat caaccggttg ttcaagggca tatcggtcag
    20641 attaaaaagg ccgtgcagat gttggcatct gccaaacgcc cggtcgtcta ttttggcggc
    20701 ggcgtggtgt tgggtaatgc ttctgaagag ctgacccgat ttgtccgaat gacgggtgct
    20761 ccgtgcacgg gtacgctgat gggcttgggc gcttatcctt ccggcgaccg ccaatttctc
    20821 ggtatgctcg gtatgcacgg tacttacgag gcaaaccttg ccatgcagaa tgcggatgtc
    20881 gttcttgccg taggtgcgcg ttttgacgac cgtgtggttt ccgtaccgtc caaatttttc
    20941 gagaaggcga agaaggttat ccatatcgat gtcgatcctt ccagcatcgc caaacgcgtg
    21001 aaggcggaca ttccgattgt cggcgacgtg aaaaacattt tgtccgagat ggttgcgctg
    21061 tggcaaaaac aagagtccgt gccgtctgaa gatgctttgg gcaaatggtg gaaaaccata
    21121 gaggaatggc gttcccgcga ttgcttgtgg tttgacaacg gcagcgaaat tatcaagccc
    21181 caatatgtga ttcagaagct tgccgagatt accggcaatt cggcaatcat cacatcggat
    21241 gtagggcagc atcaaatgtt tgcggctcaa tattatcctt tcgaacgtcc gcgccaatgg
    21301 ctcaactccg gcggtttggg tacgatgggc gtgggtctgc cttatgcgat tggtgcaaaa
    21361 cttgccgccc cggatcaaga cgtattctgt attaccggcg acggctcgat ccagatgaac
    21421 atccaagagt tgtccacctg tttccaatat cggattccgg ttaacgtcat tacgctgaac
    21481 aacggttatc tgggtatggt acgccagtgg caggaaatat attacggcgg ccgagagtcg
    21541 gaaacctatt tcgattcttt gcccgatttc gtcaaacttg ccgaggcata cggccatatc
    21601 ggcatccgcg tggacaagaa atctgatgtg gaaggtgcct tgttggaagc attgaaccaa
    21661 aaagacaggc tggtgtttat cgacttcctg accgaccaga aacagaatgt gatgcctatg
    21721 gtcggcaacg gcaaaggttt ggacgaaatg gtacttccgc cgcatatgcg tacggacgga
    21781 aaggcgtaag gagaggcaaa tgcgacatat cttatctgtt ctgatcgaaa acgaatcagg
    21841 tgcgatgagc cgcgtggtcg gtttgttctc tgcacgcgat tacaatatcg attctttggc
    21901 ggttgcgccg accgaagaca aaaccctgtc acggatgacc atcgttaccc acggcgatga
    21961 gcaggttatc gaacaaatca ccaagcaact caataaattg attgaagtga ttaaagtggt
    22021 cgatttgaac gaaagccgtt ttgtcgaacg cgaactgatg ttggtaaaag tccgtgccgc
    22081 cggcaaagac cgcgacgaat ttttacgctt gaccgaaatc taccggggca gcatcatcga
    22141 cgtaaccgac cgcagctata cgattgaaat taccggctcg accgacaagc tggattcctt
    22201 tttggaaacg gtcggacgcg cccaaatttt ggaaaccgta cgtacaggtg cggccggcat
    22261 cggtcgcggt gagcgtattt tgaaaattta acgccgtaac ctttcagacg gcatggtatt
    22321 tgaatgccgt ctgaaaaacg aacggcagga gagatttatg tcgaacatta aaattgtcgc
    22381 actggttacc gtcaaaccgg aatacacgga aacactggca gcacagttta aagaactggt
    22441 caaagccagc cgtgcggaag agggcaacat cagctacaat ctccatcagg aaatcggcaa
    22501 accgaaccgt tttgttttcg tggaaaattg gaaatcccaa gcagctattg acgaacataa
    22561 tgccagcgcg catttccaag ccttcgtcca gtccgtcgac ggcaaaaccg aagcgttgga
    22621 aatcgtattg atgaatgaag ttgccgttta agcggcacac tctgtttaac ccgtccgaag
    22681 ccgctcaaca ttttaaggct tcgacaacca tttaccttaa aggaaatcaa atgcaagtct
    22741 attacgataa agatgccgat ctgtccctga tcaaaggcaa aaccgttgcc atcatcggtt
    22801 acggttcgca aggtcatgcc catgctgcca acctgaaaga ttcgggtgta aacgtggtga
    22861 tcggtctgcg ccacggctct tcttggaaaa aagccgaagc agccggccat gttgttaaaa
    22921 ccgttgctga agcgaccaaa gaagccgatg tcgttatgct gctgctgcct gacgaaacca
    22981 tgcctgccgt ctatcacgcc gaagttgcag ccaatttgaa agaaggcgcg acgctggcat
    23041 ttgcacacgg cttcaacgtg cactacaacc aaatcgttcc gcgtgccgac ttggacgtga
    23101 ttatggttgc ccccaaaggt ccgggtcata ccgtacgcag tgaatacaaa cgcggcggcg
    23161 gcgtgccttc tctgattgcc gtttaccaag acaattccgg caaggccaaa gacatcgccc
    23221 tgtcttatgc ggctgccaac ggcggcacca aaggcggtgt gattgaaacc actttccgcg
    23281 aagaaaccga aaccgatctg ttcggcgaac aagccgtatt gtgcggtggc gtggccgagt
    23341 tgatcaaagc aggttttgaa accctgaccg aagccggtta cgcgcctgaa atggcttact
    23401 tcgaatgcct gcacgaaatg aaactgatcg ttgacctgat tttcgaaggc ggtattgcca
    23461 atatgaacta ctccatttcc aacaatgcgg agtacggcga atacgttacc ggccctgaag
    23521 tggtcaatgc ttccagcaaa gaagccatgc gcaatgccct gaaacgcatc caaaccggcg
    23581 aatacgcaaa aatgtttatc caagagggta atgtcaacta cgcgtctatg actgcccgcc
    23641 gccgtttgaa tgccgaccac caagttgaaa aagtcggcgc acgactgcgt gccatgatgc
    23701 cttggattac ggccaacaaa ttggttgacc aagacaaaaa ctgattgttt tcaaacggga
    23761 ctgcctacac atcgtgtagg cagtttgtta tatggatacc gtctgaacat cgtgttcgga
    23821 cggtatctgt gttgcggatg aatttaaaca ggcacagttc tgtcagtcgc ccagagccgc
    23881 gaccattacc gctttaatcg tgtgcatacg gttttccgcc tgatcgaaca cgatgccggc
    23941 cggactttcg aatacttctt ctgtaacttc cacaccgttc agcccgaagg tttcgtaaat
    24001 ccattcgccg actttggttt cgcggttgtg gaaggcgggc aggcagtgca tgaatttgac
    24061 ttgcggattg cccgatgccg ccatcagttc gggcgtaacg cggtaatctt tcagcaaatc
    24121 gatgcgttcc tgccagactt ctttcggctc gcccatgctg acccatacgt cagtatgaat
    24181 gaaaccgaca cctttgacgg cttcatgcgc gttttcggtc agggtaattt ttgcaccggt
    24241 ttctttggcg gcggcgtgtg cggcggcgat aatgccttca gacggccaca ggctttgagg
    24301 tgcgccgata cgcacgtcca tccccaattt tgcccctaaa atcagcaggg aattgcccat
    24361 gttgtaacgc gcgtcgccga cgtaggcaaa cgcggtttgg ttcaaaggtt tgccgctgtg
    24421 ttcgcgcata gtcagtgcgt cggcaagcat ttgtgtggga tggaactcgt tggtcagccc
    24481 gttgaacacg ggtacgcccg catattttgc caattcttcg acagtttcct gagcgaagcc
    24541 gcgatattcg atggcatcgt acattctgcc taagacgcgc gccgtgtctt tgatgctttc
    24601 tttgtgcccg atttggctgg cggacggttc cagataggtt gcatccgcgc cttggtcgcg
    24661 tgcggcgact tcaaacgcac agcgtgtgcg cgtggatgtt ttttcaaaaa tcagggcgat
    24721 gtttttccct ttcatccgct gaatctcgcg ccctgccttt ttggcgtctt tcaactcggc
    24781 ggcaaggtcg aggtaggtgg tgatttcttc cggcgtgaag tccaaaagtt tcagaaaatg
    24841 gcggtttttc aggttcactg tcgtttcctt tatgtggggg cgtgaacgcc gtatttgcag
    24901 cgtttgcgga aagaagggtt agcggaatgt tgcaatgccg tctgaaggtt gttcagacgg
    24961 cattgcggtc aatcccttac tccatacgga tgacggggat caaacattca cggtctcggc
    25021 tacttcgttg tagctgtcga tttcgttgaa gttcatatag cggtagattt tatcgccctg
    25081 ttcgttgatg atgccgatat tggcttgata ttcttcaacg gtcgggattt tacccagttt
    25141 ggagcaaatc gccgccaact ctgccgagcc gaggtaaacg aaggtgtttt tacccaagcg
    25201 gttcgggaag ttgcgggtcg aagtggacat gacagttgca ccttcgcgta cttgggcttg
    25261 gttacccata cacaacgaac agcccggcat ttccatacgc gcgccggcgc ggccgagtac
    25321 gccgtagtga ccttcgtcgg acaactcttt cgcgtccatt ttggtcggcg gcgctaccca
    25381 caggcggacg gggatgtcgc tcttgccttc caaaagtttg gaggcggcgc ggaagtggcc
    25441 gatgttggtc atacaagaac cgatgaacac ttcgtcgatt ttggtgccgg agcgttcgga
    25501 cataaagcat acgtcgtccg ggtcgttcgg gcaggcgata atcggctctt tgatgtcgtc
    25561 catgttgatt tcaatcacgg cggcgtattc ggcatcttta tccgcttcga gcaactcggg
    25621 atttgccagc catttttcca tagctttgat gcggcgttcc aaagcgcgcg gatcttgata
    25681 gccgttggca atcatatttt tcatcaacac gacgttggat ttcatgtact cgataatcgg
    25741 ctctttattg agcttcacgg tacagccggc ggcggagcgt tcggcggatg cgtcggtcaa
    25801 ttcaaaggct tgttccactt tcaaatcggg caggccttcg atttcgagga tgcggccgga
    25861 gaagatgttt ttcttaccgg ctttggcaac ggtcagcaaa ccttgtttaa tcgcgtaaag
    25921 cgggatggcg ttcaccaaat cgcgcagggt tacgcccggt tgcagcttgc cgctgaaacg
    25981 taccaatacg gactcgggca tatcgagcgg tattacgccc gttgcggcgg caaaagcgac
    26041 caagcctgaa cctgcgggga aggaaatacc gatggggaaa cgggtgtggc tgtcgccgcc
    26101 tgtaccgact gtatcaggca acagcaggcg gttgagccac gagtggatga cgccgtcgcc
    26161 cggacgcagg gacacgccgc cacgggtaga aataaaggcg ggcagttctt tatgggtttt
    26221 tacatcgaca ggtttcggat aggcagcggt gtggcagaaa gactgcatca ccatatcggc
    26281 ggagaagccc aaacaagcca agtctttcaa ctcgtcgcgg gtcatcggcc cggtcgtatc
    26341 ttgcgagccg accgtcgtca tgcgcggttc gcagtaagta cccggacgca cgccttgtcc
    26401 ttcgggcaga ccgcaggcgc gaccgaccat tttttgcgcc aaggtgaaac cgactttgct
    26461 ttcggcaggc gcttgcggca ggcggaatgc agtagaggca ggcagtttca gggcttcgcg
    26521 cgctttggcg gtcagaccgc gaccgataat caggttgata cggccgccgg cttgcacttc
    26581 gtccagcaat acttgtgatt tcaattcaaa ctcggcaaca gtctcgccgt ttttcacgat
    26641 tttaccttca taaggaagga tattgacgac atcgcccatt ttcagcgcgg aaacatcgac
    26701 ttcaatcggc agcgcgccgg agtcttcttg agtgttgaag aaaatcggcg cgattttacc
    26761 gcccaagcac acgccgccga agcgtttgtt cggcacgaac gggatgtctt cgccggtatg
    26821 ccaaatgacg gagttggtcg cggatttgcg tgaagaaccg gtaccgacca cgtcaccgac
    26881 gtaggcaacc ggatggcctt tggctttgag ttcttccaac aatttaatcg gaccgacttc
    26941 gcccggtttg tcgggcgtga tgccgtcgcg cgggtttttc agcatggcca gcgcgtgcag
    27001 cggaatatcg ggacgactcc acgcgtcggg cgcgggagag aggtcgtctg tattggtttc
    27061 gccgtcaact ttgaagacgg taacggtgat tttttcggga actttggcac gggaggcgaa
    27121 ccattcggca tctgcccaag attgcaaaac ttcctgcgcg tatttgttgc ctttttcggc
    27181 tttttcctga acgtcgtgga aggaatcgaa catcagaagc gtatgtttca agcctttggc
    27241 ggcaatgggc gcgagtttgt cgtcgtccaa gagttcgatt aaggcgtgga tattgtaacc
    27301 gccgagcatc gtacctaaga gttcggtcgc atatttgggg gaaaccagcg ggctggatgc
    27361 gctgccttcg gcaacggcag ccaggaatga ggctttgact ttggcggcat cgtccacacc
    27421 gggcggaaca cggtgggcaa gcagctcgac caagaactcg ccttcgcctg cgggcgggtt
    27481 tttcagcagc tcaaccaaat cggcggtttg ctgcgcattc aaagggaggg cgggaatgcc
    27541 gagggcggcg cgctcggcgg cggctttacg ataggcttct aacatctctt tgttcctttt
    27601 tccgtttttc ttttgtcggg ttgcaaatga tctgcgttaa atattgtaaa caacatttgt
    27661 accgctatca tagactagtt ttaacaaaaa tggaactgtt atgacaggta at
//
LOCUS       DAWTQS010000022        25435 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.022, whole genome shotgun sequence.
ACCESSION   DAWTQS010000022 DAWTQS010000000
VERSION     DAWTQS010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 25435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25435
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.022"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            <1..173
                     /locus_tag="WNA25_000944"
     CDS             <1..173
                     /locus_tag="WNA25_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003762769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5"
                     /protein_id="HFA9823721.1"
                     /translation="EPLPPSALSESEANRDLGWMLHDIDFDHGNTPHFFRTQMKDGLI
                     DVPPFYAEEVKA"
     gene            170..1036
                     /gene="cas8c"
                     /locus_tag="WNA25_000945"
     CDS             170..1036
                     /gene="cas8c"
                     /locus_tag="WNA25_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HFA9823722.1"
                     /translation="MILASLVRYYRRLATETDETGNPKVPSYGFSEEKIGWILVLDKE
                     GRLKTVVPNLTADKKPQPKLMSVPRPEKRTSGIKPNFLWDKTAYALGVEANKNKAEAK
                     EKPFTPSEKTFEAFKQYHLDLLQNSEDEGLQALCRFLQNWQPAHFAAENLPAEMLDSN
                     TAFSLEKPTALIHKREAAQTLWAGCLKSDEALESLYLISGDTAPIARLHPAIKGVFGG
                     QSSGGSIISFNKEAFSSFGKEQGANAPVSEQSAFAYTTALNYLLRRENNHCLTIGDAS
                     TVFWAEADDIVD"
     gene            1024..1128
                     /locus_tag="WNA25_000946"
                     /pseudo
     CDS             1024..1128
                     /locus_tag="WNA25_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            1121..1528
                     /locus_tag="WNA25_000947"
     CDS             1121..1528
                     /locus_tag="WNA25_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HFA9823723.1"
                     /translation="MHYNATAQAAEGFFAQVFTPSDDEQESAKIFNVLEQIGKGRPLQ
                     EIAPELSPNTRFYILGLAPNAARISVRFWLDTTFGQLAENLAHHWQDLALEPCAWKTP
                     PSIWRLLLQTAVLGKSENIQRLGNRCLPETQNP"
     gene            1476..2236
                     /gene="cas7c"
                     /locus_tag="WNA25_000948"
                     /pseudo
     CDS             1476..2236
                     /gene="cas7c"
                     /locus_tag="WNA25_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777774.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
     gene            2263..2631
                     /locus_tag="WNA25_000949"
     CDS             2263..2631
                     /locus_tag="WNA25_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823724.1"
                     /translation="MKYWLVGASWGGQGHQDQFFVENGYWVLEWESSQQPDQFAKGEK
                     IQVGDRIAIKRMKGQGSSEIKILHIGIVKGVISETNKIICVVDWIVKNLDRNVESRGC
                     FKSIHDPYDKDEWIEKIFCL"
     gene            2638..3288
                     /gene="cas4"
                     /locus_tag="WNA25_000950"
     CDS             2638..3288
                     /gene="cas4"
                     /locus_tag="WNA25_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HFA9823725.1"
                     /translation="MTALLTETQRENQDTRLIPLSALQHYAFCPRQCALIHNEQAWAE
                     NYLTAQGKALHERVDSDEPETRKGVRFERTVHVSVEKPGISGILDLVEADTKTGRLKP
                     VEYKRGKPKPDPGDEIQLCAQGLCLEEMTGQTVSEGALWYMQTRHRVPVVFSDGLRAQ
                     TLATIAAVCKLLNSGQTPPPDYGKRCKACSLAEICQPELLGKRDRSVGYVKGLFGE"
     gene            3404..3763
                     /locus_tag="WNA25_000951"
     CDS             3404..3763
                     /locus_tag="WNA25_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823726.1"
                     /translation="MTRNIEHQFSNLSDVGEKLELENPTVENVVDILVDIGHDDRVYT
                     FHDDFLGLKSGLPQDLLSKHIDELEEGDFADRYSDEIDKILDNANIIFYHLERELSED
                     DLEEIREERERLGLEDD"
     gene            3906..4529
                     /locus_tag="WNA25_000952"
     CDS             3906..4529
                     /locus_tag="WNA25_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823727.1"
                     /translation="MTQKNDFKNANFYGNTQIVNGDVHNVHTQNTPNSINENEIATYT
                     PEPIWRSPITMAWLTWASFLISLAGLLPIYNCIVKPILYLVNGKTMIQSEINNTYIFI
                     LIAIAVLFLITITLRRITKYQTRYPLVFNYAISGMGHRLSLEKISISQCPQCGGEMKY
                     YNKPIEWENIRYSDGSKKRRVTRTTPALECKRNREHWYKVDPAEDKI"
     gene            4589..5053
                     /gene="cas1"
                     /locus_tag="WNA25_000953"
     CDS             4589..5053
                     /gene="cas1"
                     /locus_tag="WNA25_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HFA9823728.1"
                     /translation="MRKLQNTLYITTQGSYLHKERETLVVEQERKKVAQLPVHSIGHI
                     FCFGNVLVSPFLLGFCGENNVNLAFFTENGCYLGRLQGRQNGNVLLRRAQYRVSEQSP
                     MPIACNIIAAKIRAIRKRARACVLSGRYTFRRKNRASAAYWIWWKWIQKQAV"
     gene            4957..5238
                     /locus_tag="WNA25_000954"
     CDS             4957..5238
                     /locus_tag="WNA25_000954"
                     /inference="COORDINATES: protein motif:HMM:NF014040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dna2/Cas4 domain-containing protein"
                     /protein_id="HFA9823729.1"
                     /translation="MRFERTVHVSAEKPGISGILDLVEMDTKTGRLKPVEYKRGKPKP
                     DPVDEIQLCAQGLCLEEMTGQTVSEGALWYMQTRHRVPVVFSDGLRPLQ"
     gene            5404..5589
                     /locus_tag="WNA25_000955"
                     /pseudo
     CDS             5404..5589
                     /locus_tag="WNA25_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696979.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Dna2/Cas4 domain-containing protein"
     gene            complement(5629..7557)
                     /gene="rpoD"
                     /locus_tag="WNA25_000956"
     CDS             complement(5629..7557)
                     /gene="rpoD"
                     /locus_tag="WNA25_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFA9823730.1"
                     /translation="MSKNQNHEEYQDDARPLTIEEQRARLRQLIIMGKERGYITYSEI
                     NDALPDDMSDADQIDNIVSMISGLGIQVTEHAPDAEDILLSDNAAVTDDDAVEEAEAA
                     LSSADSEFGRTTDPVRMYMREMGQVDLLTREDEIIIAKKIENALKNMVQAISACPGSI
                     AEILELIEKICKDEIRVDEVVEAIIDPNEVLLNELGLGHLETTAPEKPSNDNSDENED
                     DEESEEDADEVSAANLAELKQKVIGHFAQIEKDYKKMIGCLEKHHSRHKDYLAYRDAI
                     ANKLLEVRFATRQIDSLSSSLRGKVENIRKLEREIRDICLDRVHMERDYFIQNFLPEI
                     TNLQWIEEEIAKGRVWSNALDRFRHAILEKQTELADMEKETRISIEELKEINKNMVSS
                     EKETAAAKQEMIQANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYK
                     FSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQHLQETGEEPDSAKLA
                     ELMQMPEDKIRKIMKIAKEPISMETPIGDDDDSHLGDFIEDANNVAPADAAMYTSLHE
                     VTKEILESLTPREAKVLRMRFGIDMNTDHTLEEVGRQFDVTRERIRQIEAKALRKLRH
                     PTRSDRLRSFLDSEDSKL"
     gene            complement(7744..9516)
                     /locus_tag="WNA25_000957"
     CDS             complement(7744..9516)
                     /locus_tag="WNA25_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFA9823731.1"
                     /translation="MIPSDFIDELLAKTDIVGIIDEQVPLKKGGANYMACCPFHKEKT
                     PSFSVSPTKQFYHCFSCGAHGSAIGFVMEHQGLSFPEAVQFLADRVGMIVPKVRGQND
                     NPEVRAERKKKQQTLEETTAAAADFYAQQLKFNPAAKAYLDKRGLSAEVIAHYGLGYA
                     PDGWQPLAQVFQPYPNTALVDTGMVIDNEGRHYDRFRHRIMFPIRNPRGQVIGFGGRV
                     LDDSKPKYLNSPDTPLFDKGKNLYGLYEGRAAVKEAERILVVEGYMDVVALAQFGVGY
                     GVAALGTATTAEHVKILMRQADSIYFCFDGDSAGRKAAWRALENALPQLKDDKSLHFL
                     FLPEEHDPDSYIRAYGKAQFEDALLNQSKPLSEYFWEHLSDGIHLNTQEGKAELVKTS
                     SPLLVQITAPALAYLLKQRLSELVGIDPDNLAQLLGQEAPKRHVKQKNYKLPPISVKQ
                     PVMPTLVQRQIRSLLINPDWAAYIDLPDYLALDGDFACLANLAETIKNHPSVPATAQV
                     LEHMRGSPYEETIDRIFRSALQSEEMEGGGEEDCENFQIGIKKLLNELKYSQIETLKQ
                     KSLQSGLNESEKKLLLSLLTAKQN"
     gene            complement(9661..12411)
                     /gene="secA"
                     /locus_tag="WNA25_000958"
     CDS             complement(9661..12411)
                     /gene="secA"
                     /locus_tag="WNA25_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFA9823732.1"
                     /translation="MLTNIAKKIFGSRNDRLLKQYRKSVARINALEEQMQALSDADLQ
                     AKTAEFKQRLADGQTLDGILPEAFAVCREASRRVLGMRHFDVQLIGGMVLHDGKIAEM
                     RTGEGKTLVATLAVYLNALAGKGVHVVTVNDYLASRDAGIMEPLYNFLGLTVGVIISD
                     MQPFDRQNAYAADITYGTNNEFGFDYLRDNMVTDQYDKVQRELNFAVVDEVDSILIDE
                     ARTPLIISGQADDNIQLYQIMNTVPPHLVRQETEEGEGDYWVDEKAHQVILSETGHEH
                     AEQILTQMGLLAENDSLYSAANISLMHHLMAALRAHSLFHKDQHYVIQDGEIVIVDEF
                     TGRLMSGRRWSEGLHQAVEAKEGVEIKRENQTLASITFQNYFRLYTKLSGMTGTADTE
                     AFEFQSIYNLETVIIPTNRPVQRKDFNDQIFRSAEEKFEAVVKDIEECHKRGQPVLVG
                     TTSIENSELVSRLLQKAGLPHNVLNAKEHEREALIVAQAGKVGAITVATNMAGRGTDI
                     VLGGNLKHQTDAIRADETLSDEEKQAQIAALENGWQAEHDKVMEAGGLHIIGTERHES
                     RRIDNQLRGRSGRQGDPGSSRFYLSFEDPLLRLFALDRAAAILNRLAPERGVAIEHNL
                     LTRQIEGAQRKVEGRNFDMRKQVLEYDDVANEQRKVIYSQRNEILTSKDIGDLMQEIR
                     SDAVSDLVDTYMPPDSMEEQWDIPTLENRLAAEFRLQEDIQSWLKADNAIDGQDIKER
                     LIERIENEYAAKTELVGKQAMADFERNVMLQAIDNQWREHLAAMDYLRQGIHLRSYAQ
                     KNPKQEYKREAFTMFQDLWNGIKFHIASLLTSVQIEQNPVAAVEEQPVGNIQSIHSES
                     PDIEELLGQSQTDLVTEAFNPDGTDFSPEALEARGQIVHRNDPCPCGSGLKYKQCHGK
                     LA"
     gene            12543..12965
                     /locus_tag="WNA25_000959"
     CDS             12543..12965
                     /locus_tag="WNA25_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HFA9823733.1"
                     /translation="MNLEQLGRRDALLSGLLKQAGQWRRLDAAVKKLLPANLHPHFQT
                     ACIEDGRLVLLAANNMAASRLKMIAPSVLPQLAGLDASIRSVSVRLVPKPEKPPKTNT
                     LHLSKAALESFDSAAAKLEERHPELAEALEELVRKYGA"
     gene            13248..13799
                     /gene="azu"
                     /locus_tag="WNA25_000960"
     CDS             13248..13799
                     /gene="azu"
                     /locus_tag="WNA25_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HFA9823734.1"
                     /translation="MKAYLALISAAVIGLAACSQEPAAPAAEATPAGEAPASEAPAAE
                     AAPADAAEAPAAGNCAATVESNDNMQFNTKDIQVSKACKEFTITLKHTGTQPKASMGH
                     NLVIAKAEDMDGVFKDGVGAADTDYVKPDDARVVAHTKLIGGGEESSLTLDPAKLADG
                     DYKFACTFPGHGALMNGKVTLVD"
     gene            13894..14403
                     /locus_tag="WNA25_000961"
     CDS             13894..14403
                     /locus_tag="WNA25_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HFA9823735.1"
                     /translation="MNPFETQSVTFAEPIEMLYACHGKVRRFCGQIAMLSGYIAENGC
                     NQLVLQTIRQISRYFNVAAPLHHEDEEENFFPLLLQYAPQAREGVDELLRQHIGLYDN
                     WAAVSAEFAKLEADNAYIPDAEAFKRFVEGYDVHLAIEEPLFDMGKTFIPEEKLTEIG
                     KIMAARRCK"
     gene            14437..15057
                     /locus_tag="WNA25_000962"
     CDS             14437..15057
                     /locus_tag="WNA25_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase"
                     /protein_id="HFA9823736.1"
                     /translation="MLTPKSCDLFNIPFFQFAQLKKYQPESIPQIKADYKENWQIWQQ
                     LIQQVAADLSEPFAPPHIERWCNGWQVRAHFFAYFKYAQYKNSAAILSILLNRRRLSV
                     SLDWHCYKADVSPIALPEYNRWLDDFDTEKYAAFDMWHGAESEYDDYRTVAQQSESDR
                     RLQNDEDFFCIGKHIERDDLGKQDVAKWIAETVEDLLPLYEACHGK"
     gene            15170..16315
                     /gene="dapE"
                     /locus_tag="WNA25_000963"
     CDS             15170..16315
                     /gene="dapE"
                     /locus_tag="WNA25_000963"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFA9823737.1"
                     /translation="MTETQSLELAKALISRPSVTPDDRDCQKLLAERLHKIGFAAEEL
                     HFGDTKNIWLRRGTKAPVVCFAGHTDVVPTGPVEKWDSPPFEPTERDGRLYGRGAADM
                     KTSIACFVTACERFVAEHPDHQGSIALLITSDEEGDALDGTTKVVDVLKARGELIDYC
                     IVGEPTAVDKLGDMIKNGRRGSLSGNLTVKGKQGHIAYPHLAVNPVHTFAPALLELTQ
                     EVWDEGNEYFPPTSFQISNINGGTGATNVIPGELNVKFNFRFSTESTETGLKQRVHAI
                     LDKHGVQYDLQWSCSGQPFLTHAGKLTDVARTAIAETCGVEAELSTTGGTSDGRFIKA
                     IAKELIELGPSNATIHQINENVRLDDIPKLSAVYERILARLLAEKAV"
     gene            16735..17550
                     /locus_tag="WNA25_000964"
     CDS             16735..17550
                     /locus_tag="WNA25_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFA9823738.1"
                     /translation="MGALGGIVQHHTALYRSVAVYEPTVVIVRRGCKKLRWAGRELRI
                     AAGEAVGLAGGQTLDVINIPDSDGLYQAQWIAFEQETVERFAAQYGTAQAVCDAVKLP
                     HPGRMGAAFDYAAAVLADEEVPHNAAEAALCGVLAWLQHDGIGFAVYGGVNLMRQIRK
                     LITADMAADWSSAMLAQRLNCSEAALRRRLARQDTNFRTLLTDVRMMRALTLLQVTQW
                     SVAQIAGAVGYDCPSRFSARFKELFGCVPSVVRSEAEPAAYRRTGQVSVGVRP"
     gene            complement(17528..18085)
                     /locus_tag="WNA25_000965"
                     /pseudo
     CDS             complement(17528..18085)
                     /locus_tag="WNA25_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273197.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
     gene            18435..19187
                     /locus_tag="WNA25_000966"
     CDS             18435..19187
                     /locus_tag="WNA25_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFA9823739.1"
                     /translation="MITLPSLNNLPSKWDEIRHCLETRVFECGVMPYPNLTECEAGQF
                     ERDFWDNIGCAPEEYVRIRRAIRLLELRYPDSLNELVCAAIATPLGEMLAVFGVKGLC
                     LLEFVGQKYLEQEIAAVQKALRGRFVFREDGRMQFLRQELDLYFQCRLKTFATPLEMI
                     GTAFQKQAWDVLLAIPYGETRSYKEQAQRLGNPKAVRAVAAANGQNKVSVMIPCHRVI
                     GSDGKLTGYAGGLNRKQFLLALERGEVQTALF"
     gene            19378..19453
                     /locus_tag="WNA25_000967"
     tRNA            19378..19453
                     /locus_tag="WNA25_000967"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19411..19413,aa:Asn,seq:gtt)
     gene            19584..20594
                     /gene="waaF"
                     /locus_tag="WNA25_000968"
     CDS             19584..20594
                     /gene="waaF"
                     /locus_tag="WNA25_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HFA9823740.1"
                     /translation="MSIKILIISPSWIGDCVMTQPLFRRLKELHPGCTIDVFAPKWSM
                     AVFERMPEVNEILENPFGHGALELKRRWRVGRELGRRGYDRVIVLPGSLKSAVIALAT
                     GIGKRTGYVGESRYFLLNDIRRLDKERLPLMVDRYTALAHQSQEDFDGHSGFPEFSID
                     ERRREISIETFGLNLGKPVLAFCPGAEFGPAKRWPARHFAELGKHYSEAGWQVWLFGS
                     QKDDEIAEEINCLSDGMCVNLCGKTDLSQAMDLLSLADTVVCNDSGLMHLAAALGRKV
                     VAVYGSSSPTHTPPLSDRAKIVSLHLECSPCFKRECPLGHTDCLNRLYPEKIVQAVEE
                     AV"
     gene            20649..21095
                     /gene="smpB"
                     /locus_tag="WNA25_000969"
     CDS             20649..21095
                     /gene="smpB"
                     /locus_tag="WNA25_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFA9823741.1"
                     /translation="MAIANNKKAFHDFFIEDRIEAGLVLEGWEVKAIRAARVQLKESY
                     IYWKKDAFYLVGCHITALPTASTHIKPDAVRPRKLLLKQSEINKLIGKTERAGYTIVP
                     LDLHFSRGKIKMEIGLAKGKKQHDKRQSMKEADWKREKQRLIKHTR"
     gene            21460..23499
                     /locus_tag="WNA25_000970"
                     /pseudo
     CDS             21460..23499
                     /locus_tag="WNA25_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374913.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(23482..23778)
                     /locus_tag="WNA25_000971"
     CDS             complement(23482..23778)
                     /locus_tag="WNA25_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823742.1"
                     /translation="MVGLARARGRGELDRIEQMDLDFFHRTRARYLELVKDNPKAVVI
                     NAEQTIELVQAEIESAVKIGGNYTKNDRTFKIIKSAVNFPADFILLQFNLKRPL"
     gene            complement(23828..25195)
                     /locus_tag="WNA25_000972"
     CDS             complement(23828..25195)
                     /locus_tag="WNA25_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFA9823743.1"
                     /translation="MPDLHTEFSRLLPADEIAEPSPTLLKDQRNRFTSAPDIILQPRS
                     VESVQTIMRFCYEHRIPVTPQGGNTGLCGAAVSENGVLLNLSKLNRIRSINLSDNCIT
                     VEAGSVLQTVQQAAEASNRLFPLSLASEGSCQIGGNIACNAGGLNVLRYGTMRDLVIG
                     LEVVLPNGELVSHLHPLHKNTTGYDLRHLFIGSEGTLGIITAATLKLFARPSDKATAW
                     VGIPDIESAVRLLTETQAHFAERLCSFELIGRFAAELSSEFSKLPLPTHSEWHILLEL
                     TDSLPDSNLDDRLVEFLYKKGFTDSVLAQSEQERIHMWALRENISASQRKLGTSIKHD
                     IAVPIGRVADFVRRCAKDLEQNFKGIQIVCFGHLGDGSLHYNTFPPEILSNEVYRYEN
                     DINSTVYRNVLARNGTIAAEHGIGIIKKQWLPAVRTPSEIALMKSIKQHLDPYNIMNP
                     GKLLP"
BASE COUNT         6151 a         5944 c         6878 g         6462 t
ORIGIN      
        1 gtgaaccgct gccgccgtcg gcgttatcgg aaagcgaggc aaaccgcgat ttgggctgga
       61 tgctgcacga tattgatttt gatcacggca acacgccgca ttttttccgc acgcaaatga
      121 aagacggctt gattgatgtg ccgccgtttt acgccgagga ggtgaaagca tgattcttgc
      181 gtcccttgtc cgctattacc gccgtttggc aacggaaacc gatgaaacgg gcaacccgaa
      241 agtgccgtct tatggtttta gcgaggagaa aatcggctgg attttggtgt tggataaaga
      301 aggtcgtctg aaaaccgttg tgccgaatct gactgccgat aaaaagccgc agccgaagct
      361 gatgagtgtg ccgcgccctg aaaaacgcac gtcgggtatc aaaccgaatt ttttgtggga
      421 taaaaccgcc tacgcgcttg gcgtggaagc caataaaaac aaagccgaag ccaaagaaaa
      481 accgtttacg ccgtctgaaa aaacctttga agccttcaag caataccatc tcgatttact
      541 gcaaaacagc gaagacgaag gtttacaagc cttatgccgt tttctgcaaa actggcagcc
      601 tgcacatttc gctgccgaaa acctgcctgc cgaaatgctt gattccaaca ccgcattttc
      661 tcttgaaaaa ccgaccgctc ttatccataa acgcgaagcc gcgcaaacct tgtgggcagg
      721 ctgcctgaaa agtgatgaag cactcgaaag cttatacctg attagcggcg acaccgcgcc
      781 gattgcacgg ctgcatccgg cgattaaagg cgtgtttggc gggcaaagct ccggcggttc
      841 gattatttcg tttaataaag aagccttttc ctctttcgga aaagagcaag gcgcaaatgc
      901 gcctgtttcc gaacaatccg cctttgccta caccaccgcg ctgaactatc tcttgcgccg
      961 cgaaaataat cactgcctga ccataggcga tgccagcacg gtcttttggg cggaagcgga
     1021 tgatatagtg gattaaattt aaatcaggac aaggcgacga aaccgcagac agtacaaata
     1081 gtacggcaag gcgaggcaac gccgtaccgg tttaaattta atccactata acgccacggc
     1141 gcaggctgcc gaaggcttct tcgcgcaagt gtttacgccg tcggatgacg aacaagaaag
     1201 cgccaaaatt ttcaacgtat tggaacaaat cggcaaaggt cgtccgctgc aagaaattgc
     1261 gcctgaactc tctcccaata cccgttttta catcttgggg cttgccccca atgctgcgcg
     1321 gatttctgtt cggttttggc tggacaccac atttgggcag ctggcggaaa acttggcgca
     1381 tcattggcaa gatttagccc ttgagccttg tgcgtggaaa acgccgccgt ctatttggcg
     1441 gctcttgttg caaactgccg tgctgggcaa aagcgaaaac atccaaaggc ttggtaaccg
     1501 atgtctgcct gaaacgcaaa atccgtaatt ttgtcgaaat cagcagcgaa aacgaagccg
     1561 gttacgaaat ctacgtcaaa gaaaaaagcg tgctgaacct gcaaaacaaa cgcgcttatg
     1621 aggcgcttgg cattgagtcg gaagccaaaa actgccgaag gacgaagcca aagcccgtga
     1681 tattaccgct tggatgtgta aaaacttctt tgacatccgc accttcggcg ccgtgatgac
     1741 caccgaagtc aacagcggac aagtgcgcgg cccggtacaa ctggcgtttg cccaatccat
     1801 cgacccgatt gtgccgccgg aagtttccat cacccgcatg gcggtaacca atgaaaaaga
     1861 cttggaaaaa gaacgcacca tggggcgcaa atacatcgtc ccttacgcgc tctaccgcgt
     1921 gcatggcttt atctccgcca accttgccgc caaaaccggt ttttcagacg acgacttagc
     1981 caaactctgg caagccctga cgttgatgtt tgaacacgac cgctccgccg cccgtggcga
     2041 aatggcggca cgcaaactgg ttgttttcaa acacgacagc gcactcggca gccagcctgc
     2101 acataaactg tttgacgccg tgaaagtcga acgcgtaaac ggcgaatcag gtacgcccgc
     2161 aagcggtttt ggcgattaca aaatcagcgt ggtttcagac gggctaaatg gcgtgagtgt
     2221 ggaagagtat ttgtaagttt tcaataaatc aggagtaacg atatgaaata ttggctagtt
     2281 ggagcctctt ggggagggca ggggcatcaa gatcaatttt ttgttgaaaa tggttactgg
     2341 gttttggagt gggaatcctc tcaacaacca gatcaatttg caaaaggaga aaaaatccaa
     2401 gtaggtgatc gaattgcaat caaacgcatg aaaggtcaag gatcgtctga aattaagatt
     2461 ttacatattg gaatcgttaa gggggttatt tctgaaacga acaaaattat ctgtgtcgtt
     2521 gattggatag ttaaaaattt agatagaaat gttgaaagta gaggatgctt taaaagtatt
     2581 catgatccat atgataaaga tgaatggata gaaaaaattt tttgtttgta gcaaaagatg
     2641 accgcacttt taaccgaaac ccaaagggaa aatcaggata cgcgcctgat tcccctttcc
     2701 gccctgcaac actacgcctt ctgcccgcgt caatgtgctt tgattcacaa cgaacaggcg
     2761 tgggcggaga actatttgac cgcgcaaggc aaagcgttac atgagcgggt ggattcggac
     2821 gagccggaaa cgcgcaaggg cgtacgtttt gagcggacgg tacacgtttc ggtagaaaaa
     2881 ccgggcatca gcggcatatt ggatttggtg gaagcggata cgaaaacagg ccgtctgaaa
     2941 cctgtggaat acaaacgagg caagcccaaa cctgacccgg gggatgaaat ccagctttgc
     3001 gcccaaggtt tgtgtttgga agaaatgacg gggcaaaccg tttcagaggg cgcgctgtgg
     3061 tatatgcaaa cccgccaccg cgtccccgtt gtgttttcag acggtctgcg cgcccaaacc
     3121 ctcgccacca tcgccgctgt gtgcaaactc ctaaacagcg gccaaacccc gccgcccgac
     3181 tacggcaaac gctgcaaagc ctgctcgctg gcggagattt gccagccgga gttgctggga
     3241 aaacgggata ggagtgtggg atatgtgaag gggttgtttg gggagtaata ttttatttga
     3301 aaaaaattat tacgttaatt aacttgaatc ttccaaatta atcgcaattt ctgaaaacga
     3361 gcaatcatgc tcaattgtaa ttacacattt taaggagtcg taaatgacaa gaaatataga
     3421 acatcaattt agcaatctat ctgatgttgg tgaaaaacta gagttagaaa atcctactgt
     3481 agagaatgtc gtagatattt tagtggatat tggacatgat gaccgagttt atacttttca
     3541 tgatgacttt ctcgggttaa aaagcggttt gccacaagac ttattgtcga aacatatcga
     3601 cgagcttgaa gaaggtgatt ttgcagatag atattcagat gaaattgata aaatactgga
     3661 taatgcaaat ataatattct atcaccttga acgagagctt agtgaagatg atttagagga
     3721 aatacgagaa gaaagagaaa gactgggttt agaagacgat tagtatactt gttgtcatag
     3781 aatagattca atacatacag gttgggttat gctgttttac ccaaccagct aacctcaggg
     3841 catatttaat attatttaat tctgaataat attctattga agtttcaaga tatctaagga
     3901 aaaacatgac acaaaaaaat gattttaaaa atgctaattt ttatgggaat actcaaatag
     3961 ttaatggtga tgttcataat gtacatactc aaaatacacc aaattcaata aatgaaaacg
     4021 aaatagccac atacaccccg gaaccaattt ggagaagccc cattacgatg gcttggctga
     4081 catgggctag ttttctgatt tctttagcag ggctattacc tatttataat tgtatagtta
     4141 aacccatatt atatttagta aatggtaaaa ctatgataca aagtgaaata aataatactt
     4201 atatatttat tttaatagct attgctgtat tatttcttat cactattaca ttacgcagaa
     4261 ttactaagta tcaaactaga tacccattag tttttaatta tgctattagc ggtatggggc
     4321 atcgtttaag tctagagaaa attagtattt ctcaatgtcc acagtgcggt ggagaaatga
     4381 aatattataa taagccaatc gagtgggaga atatacgtta ttctgatggt agcaagaaaa
     4441 gaagggtaac aagaactaca ccagcattag agtgtaaaag aaatcgtgag cattggtata
     4501 aagttgatcc ggcagaagat aaaatctaat ttttagctag cccctcgttt aaccccaaaa
     4561 gcacggtcaa aacctaagga gttttcccat gcgcaaactg caaaacacac tctacatcac
     4621 cacccaaggc agctatctgc ataaggagcg ggagacactg gtggtggagc aggagcgtaa
     4681 gaaggtggcg cagttaccgg tgcattccat cgggcatatt ttctgttttg ggaatgtgct
     4741 tgtgtcgccg tttctgttgg ggttttgcgg cgaaaataat gtgaatttgg cgttttttac
     4801 cgaaaacggg tgttatttgg gacggcttca ggggcggcag aacggcaatg tgctgttgcg
     4861 tcgggcgcag tatcgggtgt cggagcaaag tcccatgccg attgcgtgca atatcattgc
     4921 ggcaaagatt cgggcgatcc ggaaacgtgc aagggcgtgc gttttgagcg gacggtacac
     4981 gtttcggcgg aaaaaccggg catcagcggc atattggatt tggtggaaat ggatacaaaa
     5041 acaggccgtc tgaaacctgt ggaatacaaa cgaggcaagc ccaaacctga cccggtggat
     5101 gaaatccagc tttgcgccca aggcttgtgc ttggaagaaa tgacggggca aaccgtctct
     5161 gagggcgcgc tgtggtatat gcaaacccgc caccgtgtcc ccgtcgtgtt ttcagacggc
     5221 ctgagacctt tgcaataaca taggttacta aaattttatg ctcaatctca ttttcaaaat
     5281 gcaaaacttt tctgattttt cctacttttt gctcaatatt aggaaggttt taggcaattg
     5341 aaaatttttt ggcgcatttt tatgcgtcaa atttcgttaa cagactattt ttgcaaaggt
     5401 ctcggcctac gcgcccaaac cctcgcaact atagccgccg tccgcgaact cttaaacagc
     5461 ggacaaaccc cgccgcccga ctacggcaaa cgctgcaaag cctgctctct ggtggaagtc
     5521 tgtcagccgg agttgttgga gaaacgggat gggagtgtgg gatatgtgga agcgttgttt
     5581 attgtgtaag aaaaaaccgc aggtatttga aacctgcggt tttttggttt acagcttgct
     5641 gtcttcgctg tccaagaaac ttctcaaacg gtcgcttctt gtcggatgcc gcagcttgcg
     5701 gagtgctttt gcctcgattt gacggatgcg ttcgcgcgtt acgtcaaact gtctgccgac
     5761 ttcttccagc gtgtggtcgg tgttcatatc gatgccgaaa cgcatacgca ggacttttgc
     5821 ctcacgcggt gtcaggcttt cgaggatttc tttggttact tcgtgcaggc tggtgtacat
     5881 tgccgcatcg gccggcgcaa cattgttggc atcttcgatg aagtcgccca agtgcgaatc
     5941 gtcgtcgtcg ccgatggggg tttccatcga aatcggctct ttggcgattt tcatgatttt
     6001 gcggattttg tcttcgggca tctgcatcag ttcggcaagt ttggcggaat cgggttcttc
     6061 gccggtttct tgaaggtgtt ggcgcgagat gcggttcatc ttgttgatgg tttcaatcat
     6121 atgtaccgga atacggatgg tacgcgcctg atcggcaatc gagcgggtaa ttgcctggcg
     6181 gatccaccag gttgcgtagg tggagaattt gtagccccgg cggtattcga acttgtcgac
     6241 cgccttcatc aggccgatgt tgccttcctg aatcaaatca aggaactgca agccacggtt
     6301 ggtgtatttt ttggcaatgg aaatgacgag gcgcaagttt gcctgaatca tttcctgttt
     6361 ggcggctgcg gtttcttttt cgctcgacac catatttttg ttgatttctt tcaactcttc
     6421 gatggaaatg cgggtttctt tttccatatc cgccaactcg gtttgttttt cgaggatggc
     6481 gtggcggaag cggtcgagtg cgttgctcca aaccctgcct ttggcgattt cttcttcaat
     6541 ccattgcaga ttggtgattt cgggcaggaa gttttggatg aagtagtcgc gttccatatg
     6601 gacgcggtcg aggcagatgt cgcggatttc gcgttcgagt ttgcggatgt tttctacttt
     6661 tccgcgcagg ctgctgctga ggctgtcgat ttgccgggtg gcgaaacgga cttccagcag
     6721 tttgttggca atcgcgtcgc ggtaggcgag atagtctttg tgccggctgt ggtgtttttc
     6781 caaacagccg atcatttttt tgtagtcttt ttcgatttgg gcaaagtggc cgatgacttt
     6841 ttgtttcaat tcggcgagat tggctgccga gacttcatcc gcatcttctt ccgattcttc
     6901 gtcgtcttcg ttttcgtccg aattgtcgtt ggaaggtttc tcgggtgctg tggtttccaa
     6961 gtgccccaag cccaattcgt tgagcaatac ttcattcggg tcgataatgg cttctacgac
     7021 ttcgtcgacg cggatttcgt ctttgcagat tttttcgatg agttctaaga tttccgcaat
     7081 ggaacccggg caggcggaga tggcctgaac catatttttc agggcgtttt cgattttttt
     7141 ggcgatgatg atttcgtctt cgcgggtcag caggtcgacc tgtcccattt cgcgcatata
     7201 catacggacg gggtcggtcg tcctgccgaa ctcggaatct gcgctggaaa gggcggcctc
     7261 ggcttcttcg acggcatcat cgtcggtaac ggcggcattg tcgcttaaca atatgtcttc
     7321 cgcatcaggg gcgtgttcgg taacttggat acccaaaccg gaaatcatac tgacgatgtt
     7381 gtcgatttga tcggcatcgg acatatcgtc gggcagtgcg tcgttgattt cggagtaggt
     7441 gatgtagccg cgttctttgc ccataatgat gagttggcgc aggcgcgcac gttgttcctc
     7501 aatcgtcagg gggcgggcat cgtcttggta ttcttcgtga ttttggtttt tggacattgt
     7561 ctgctttctg ttggcaagat gccgacggtt tgcgtttgga cgcacctgaa ggcgtggcaa
     7621 agtagggtac tctgatgccg tctgaaggct ttttgggatg ggtgttctgc aggtgtcgtt
     7681 gttgcagccg gggtgaatgc ttttcagacg gcataacggt ttacagatgg cggaatccgc
     7741 cggtcaattt tgttttgcgg tcagcagcga cagcaaaagt tttttctcgc tttcatttaa
     7801 gccggattgc aggctttttt gttttaatgt ttcaatttgg ctgtatttta actcattgag
     7861 cagttttttg atgccgattt ggaagttttc gcaatcttct tcgccgccgc cttccatttc
     7921 ttccgattga agggccgacc ggaagatgcg gtcgattgtt tcttcgtagg gcgaaccgcg
     7981 catatgttcc aaaacctgtg cggttgccgg cacggaagga tggtttttaa tggtttcggc
     8041 gaggttggca aggcaggcga aatcgccgtc caacgccaga taatcgggca ggtctatata
     8101 tgcagcccaa tccggattta tcaagaggct gcggatttgc cgttgcacca atgtcggcat
     8161 aacgggctgt ttgacggaaa tcggaggcag tttgtagttt ttttgtttga cgtgccgctt
     8221 tggcgcttcc tgtccgagca gttgcgcgag gttgtcgggg tcgatgccga ccagctcgct
     8281 aagccgttgt tttaacaaat aagccaatgc cggtgcggta atctgcacca aaagcggcga
     8341 actggttttc accaattccg ccttgccttc ctgcgtattg agatgaatgc cgtctgaaag
     8401 gtgttcccag aaatattccg acaaaggctt gctttgattc agaagcgcgt cttcaaattg
     8461 cgctttgccg taggcgcgga tgtagctgtc ggggtcgtgt tcttccggca ggaataaaaa
     8521 atgcagcgat ttgtcgtctt tcaactgcgg cagcgcgttt tccagcgcgc gccaagccgc
     8581 ttttcgcccc gcgctgtcgc cgtcgaaaca gaaataaata ctgtccgcct gccgcatcag
     8641 gattttgacg tgttccgccg tcgtcgccgt gcccaaggcc gccacaccgt agcccacgcc
     8701 gaactgtgcc agcgcgacca cgtccatata gccttcgacc accaaaatcc gttctgcctc
     8761 ttttacagca gcacgcccct catacaagcc gtaaaggttt ttccccttat cgaacaaagg
     8821 cgtatcgggc gaattcaaat atttcggttt cgagtcgtcc agcaccctgc cgccgaaacc
     8881 gatgacctgc ccgcgcggat tgcggatggg gaacataatc cgatggcgga agcggtcgta
     8941 atgccgcccc tcattgtcaa tcaccatccc cgtatccacc aacgcggtat tcggatacgg
     9001 ttggaacact tgcgccaaag gctgccagcc gtcgggcgcg tagcccaaac cataatgcgc
     9061 gatgacttcc gcgctcaagc cgcgtttgtc caaataagct ttcgcagccg gattgaattt
     9121 cagctgctgc gcgtaaaaat cagctgccgc cgccgtcgtt tcttccagcg tctgctgttt
     9181 tttcttgcgc tcggcacgga cttcgggatt atcgttctgc ccgcgcactt taggcacaat
     9241 catacctacg cggtcggcaa ggaactgaac cgcctccgga aacgacagtc cctgatgttc
     9301 catcacaaaa ccaatcgccg aaccatgcgc cccgcaactg aaacaatgat aaaactgctt
     9361 ggtcggactg accgaaaacg acggtgtttt ttccttgtgg aacgggcaac acgccatata
     9421 gttcgccccg ccttttttca gcggaacctg ctcgtcgatg atgccgacaa tatcggtttt
     9481 ggccagaagc tcgtcaatga agtcggatgg aatcatgatg tggggggacg ggatgtctgt
     9541 tttcggtacg gacggtacga aatcgggatt tttcggaatg gcggtcggat gtggcggttc
     9601 aggcaaaatg ccgtctgaag ccggaaaccg gatgttcaga cggcatttgc gttcaaacgc
     9661 ttaagccaat ttgccgtggc attgtttgta tttcaaaccg ctgccgcagg ggcaggggtc
     9721 gttgcggtgg acgatttgtc cccgcgcttc caaggcttcg gggctgaaat ccgtcccatc
     9781 gggattaaag gcttcggtaa ccagatcggt ttgcgactga cccaaaagtt cttctatatc
     9841 gggcgattcg gaatggatgg actggatgtt gccgacgggt tgctcttcaa ccgccgcgac
     9901 agggttttgt tcgatttgaa ccgaagtaag cagggaggcg atatggaatt tgatgccgtt
     9961 ccacaggtct tggaacatgg taaaggcttc gcgtttatat tcctgcttcg gatttttctg
    10021 ggcatagctg cgcaggtgta tgccttgtcg caggtagtcc atggcggcaa gatgttcgcg
    10081 ccattggttg tcgatggcct gcaacatcac gttgcgctcg aaatcagcca ttgcctgctt
    10141 gccgaccagt tcggttttgg cggcatattc gttttcgata cgttcgatca ggcgctcttt
    10201 gatgtcttga ccgtcaatcg cattgtccgc cttcagccag gattggatgt cttcctgaag
    10261 cctgaattcg gcagccaggc ggttttccag tgtcgggatg tcccattgtt cttccatgct
    10321 gtcgggcggc atataggtat ccacgaggtc gctgacggca tcagaacgga tttcctgcat
    10381 caggtcgccg atgtctttgc tggtcagaat ttcgttgcgc tggctgtaaa tgactttgcg
    10441 ctgttcgttg gcaacgtcgt cgtattccaa aacctgtttg cgcatatcga agtttctgcc
    10501 ttcgactttg cgttgcgccc cttcgatttg gcgcgtcagc aggttgtgct cgatggcaac
    10561 gccgcgttcg ggggcgaggc ggttgaggat ggcggcggcg cggtcgagtg cgaagaggcg
    10621 caacagcggg tcttcaaacg agaggtagaa acggctggat ccggggtcgc cctgacggcc
    10681 ggaacgtccg cgcaactggt tgtcgatacg gcggctttcg tggcgttccg taccgatgat
    10741 gtgcaaaccg cctgcttcca tcactttatc gtgttctgcc tgccagccgt tttcgagtgc
    10801 ggcgatttgt gcctgtttct cttcgtcgct caaggtttcg tcggcgcgga tggcatcggt
    10861 ttggtgcttc aggttgccgc ctaaaacgat gtccgtaccg cgtcccgcca tattggtggc
    10921 gacggtaatt gcgccgactt tgccggcttg ggcgacaatc agggcttcgc gttcgtgttc
    10981 tttggcgttg aggacgttgt gcggcagtcc ggctttttgc agaaggcggg agaccagttc
    11041 ggagttttcg atgctggtcg taccgacgag gacgggctgc ccgcgtttgt ggcattcctc
    11101 gatgtctttg acgacggctt cgaatttttc ttcggcggaa cggaaaatct ggtcgttgaa
    11161 gtctttgcgc tgtacggggc ggttggtcgg gatgatgacg gtttcgaggt tgtagatgct
    11221 ttggaactcg aaagcctcgg tatctgccgt accggtcata ccggagagct tggtgtacag
    11281 gcggaaatag ttttggaagg tgatggaggc gagggtttgg ttttcgcgtt tgatttccac
    11341 gccttctttg gcttctacgg cttgatgcag gccttcggac cagcggcggc cggacatcag
    11401 acggcctgta aattcgtcaa cgatgacgat ttcgccgtct tggataacgt aatgttggtc
    11461 tttgtggaac agcgagtgcg cgcgtaatgc tgccataagg tggtgcatca gggagatatt
    11521 tgccgccgag tacagcgagt cgttttctgc cagcagcccc atttgggtca ggatttgttc
    11581 ggcgtgttcg tgacctgttt cgctcaggat gacctgatgt gccttttcgt cgacccaata
    11641 gtcgccttcg ccttcttctg tctcttgacg gacgaggtgg ggcggaacgg tgttcatgat
    11701 ttggtagagc tggatgttgt cgtctgcctg accggagata atcagcgggg tgcgcgcttc
    11761 gtcaatcaag atggagtcca cttcatcgac aacggcaaaa ttcaattcgc gctgcacttt
    11821 gtcgtattgg tcggttacca tattgtcgcg caggtagtcg aagccgaatt cgttgttcgt
    11881 gccgtaggta atgtcggcag cgtaggcgtt ttgacggtcg aacggctgca tatctgaaat
    11941 aatcacgccg acggtcagac cgaggaaatt gtagagcggc tccataatgc ctgcgtcgcg
    12001 cgaggcgagg tagtcgttga cggtaacgac gtgcacgcct ttgccggcca gcgcgttgag
    12061 atagacggcg agggtggcga ccagggtttt gccttcgccg gtacgcattt cggcgatttt
    12121 gccgtcgtgc agcaccatac cgccgataag ctgcacgtcg aagtggcgca taccgagtac
    12181 gcggcgggac gcttcgcggc agacggcgaa ggcttcgggc aaaatgccgt ccaaagtctg
    12241 accgtcggcg aggcgttgtt tgaattcggc agttttggct tgcagatcag catcgcttag
    12301 ggcttgcatc tgttcttcga gcgcgttgat tctggcaacg gatttacggt attgtttcag
    12361 caagcggtcg ttgcggctgc cgaagatttt cttggcaatg tttgtcagca tgaataattc
    12421 ctgcgcctga tataatagtg gggcgaaaac gcgattttaa cacaagcgtt tgtgcaaagt
    12481 gctaatgccg tctgaaataa aggcgcgttt gcggtatttc aatcttttgg ggtgtatgtt
    12541 ttatgaattt ggaacagttg ggcaggcggg acgcgctgct ttccggactt ttgaaacagg
    12601 cgggacagtg gcggcggttg gatgctgccg tgaaaaaact gctgcctgcc aacctgcacc
    12661 cccactttca gacggcatgt atcgaagacg gcaggcttgt ccttttggcg gcaaacaata
    12721 tggcggcatc gcgtttgaag atgattgccc cgtcggtatt gccgcagctg gcagggcttg
    12781 atgcttcgat acggtctgtt tcggtcaggc tggttccaaa accggaaaaa ccgccgaaaa
    12841 ccaataccct gcatttgagt aaggctgcgt tggagagttt cgattcggcg gcggcaaagt
    12901 tggaagaacg gcatcccgaa ttggcggagg cactggagga gctggtcagg aagtacgggg
    12961 cataaggcgg ccggcaggga tgttgtaaat atccgttaat atttgcgcgg cgggggacgg
    13021 ctgccctaat actaaagaaa taggttcggg gtaagattgc cctttttttg ggtaaacgat
    13081 tgtaaacttg caaacaggct ttgatttcaa atgaagtttg tagcaaaatg ccgccccgaa
    13141 acatctgttt gtgcaacacg gcggaatctt tttcaaggtt ttgttaatgg cggttgcact
    13201 ttgatttccg taaaaccgaa tattatttta tcgattggag atttaccatg aaagcttatc
    13261 tggctctgat ttctgccgcc gttatcggtt tggctgcctg ctctcaagaa cctgccgcgc
    13321 ctgctgccga ggcaactcct gccggtgaag cacccgcttc cgaagcgcct gccgccgaag
    13381 ctgctcctgc agatgctgcc gaagcccctg ctgccggcaa ctgtgcggca actgtcgaat
    13441 ccaacgacaa tatgcagttc aacaccaaag acatccaagt cagcaaagca tgtaaagagt
    13501 tcaccatcac tctgaaacat accggtacgc aacccaaagc cagcatgggt cacaaccttg
    13561 tgattgccaa agctgaagac atggacggcg tatttaaaga cggcgtaggt gctgccgata
    13621 ccgactatgt caaacctgac gatgcgcgcg ttgttgccca caccaaactg atcggcggcg
    13681 gcgaagagtc ttccctgact ttggatcctg ccaaattggc tgacggcgac tacaaatttg
    13741 cctgcacctt cccgggtcac ggtgctttga tgaacggcaa agtgactttg gtcgattaat
    13801 ccgcttaaag tttcaaaaga cagacagcct gctttgtgca ggctgtttta ttataaaatg
    13861 actgcttgaa aagtccccgt tgagaacgaa aacatgaatc cgtttgaaac ccaaagcgtt
    13921 acctttgccg aaccgattga aatgctgtat gcctgccacg gcaaagtgcg ccgtttttgc
    13981 ggccagattg ctatgttgtc gggctatatc gccgaaaacg gctgcaatca gcttgttttg
    14041 caaaccatcc gccaaatctc ccggtatttc aacgttgccg cgccgctgca ccatgaggac
    14101 gaagaagaaa actttttccc gctgctgctg caatacgcgc cgcaagcccg tgaaggcgtg
    14161 gacgagctgt tgcgccaaca tatcgggctg tacgacaact gggcggctgt ttccgccgaa
    14221 tttgccaaac tcgaagcaga caacgcttat atccccgatg cggaagcgtt caaacgcttt
    14281 gtggagggat atgatgtgca tctggcgatt gaagagcctt tgttcgacat gggcaaaacg
    14341 tttatccccg aagagaagct gaccgaaatc ggcaaaatca tggcggcgcg ccggtgcaaa
    14401 taatgccgtc tgaaacaatc gtctcaagga tatattatgc tgacgcccaa aagctgcgat
    14461 ttgttcaata tcccgttttt ccagtttgcc cagctcaaaa aataccagcc cgagagcatt
    14521 ccgcaaatca aagccgacta caaggaaaac tggcagatat ggcagcagtt gatacagcag
    14581 gtcgccgcag atttgagcga acctttcgcc ccgccgcata tcgaacgctg gtgtaacggc
    14641 tggcaggttc gcgcccattt cttcgcctac ttcaaatacg cccaatacaa aaattccgcc
    14701 gccatcctgt cgatactgct caaccgccgc cgcctgagcg tcagcttgga ctggcattgc
    14761 tacaaagccg acgtgtcgcc gattgcgctg cccgagtaca accgctggct ggatgatttc
    14821 gataccgaaa aatacgccgc attcgatatg tggcacggcg cggaaagcga atatgacgac
    14881 taccgcaccg tcgcacaaca aagcgaatcc gaccgaaggt tgcaaaacga cgaagacttt
    14941 ttctgcatcg gcaaacacat tgagcgcgac gatttgggca agcaggatgt agcaaaatgg
    15001 atagccgaaa cggtggaaga tttactgccg ctttacgaag cctgtcacgg caaatgattc
    15061 agaaaactga cgggtacgaa tgcccgtctt atacaccaat ccaacccgga ggtcgtctga
    15121 aaatcagctt caggacattt cagacgacct gatttccaaa gtgaacaaaa tgaccgaaac
    15181 ccaatcccta gaactcgcca aagcgttgat ttcccgcccg tccgttaccc ccgacgaccg
    15241 agattgccaa aaactgcttg ccgaacgcct gcacaaaatc ggttttgcgg ctgaagaact
    15301 ccatttcggc gacaccaaaa acatctggtt gcgacgcggc acgaaagctc ctgtcgtctg
    15361 ttttgcaggg cataccgacg ttgtgccgac aggtcctgtt gaaaaatggg attcgccccc
    15421 gttcgaaccg accgagcgcg acggaagatt atacgggcgc ggcgcggcgg acatgaaaac
    15481 cagcatcgcc tgtttcgtta ccgcctgcga acgctttgtt gccgaacatc ccgaccatca
    15541 aggcagcatt gcactcttga ttacttccga cgaagagggc gatgcactgg acggcacgac
    15601 caaagtcgtc gatgtgttga aagcacgcgg agagttgatt gactactgca tcgtcggcga
    15661 accgaccgcc gtggacaaat tgggcgatat gattaaaaac ggccggcgcg gttcgctgtc
    15721 gggcaatctg accgtcaaag gcaagcaagg gcatatcgcc tatccgcatt tggcggtgaa
    15781 tcccgtgcat acttttgccc cggccttgtt agagttgacg caggaagttt gggacgaagg
    15841 caacgaatac ttcccgccga ccagctttca aatttccaat atcaacggcg gcacaggcgc
    15901 gaccaacgtc attccgggcg agctgaacgt caaattcaat ttccgcttct ccacagagtc
    15961 caccgaaaca gggctgaaac aacgcgtcca cgccattttg gacaaacacg gcgtgcaata
    16021 cgatttgcag tggtcgtgtt cgggacagcc cttcctgacc cacgcgggca aactgaccga
    16081 cgtggcacgc accgccattg ccgaaacctg cggcgttgag gccgaattgt ccaccaccgg
    16141 cggcacttcg gacggacgct tcattaaagc cattgcgaaa gaactcatcg aattaggccc
    16201 gtccaatgcc accatccacc aaatcaacga aaatgtgcgg ctggacgata ttccgaagct
    16261 gtcggcggtg tatgagagga tattggcgcg gttgttggct gaaaaggccg tctgaaaaga
    16321 agatgggctg gttgtcggat atggatgtcc gacaatctga ttagacccaa catgagtttg
    16381 agataacgca ttgattttta tgtagagcga tttatttgaa cggtaatcca ttccctctcc
    16441 cgtgggagag ggttagggag agggcattgg gacggcaacg gaaatattgt ctgtatctgc
    16501 cacgttttac cctccctccg accctcccca caggggaggg agctaagttg caggcacacc
    16561 gacggaatat ggttgaaacc ctgcccttca gggcagtaga attttggttt attttttggg
    16621 aagtaacccc ttccaaatca gggtaaacag ggctgcgcct tatgtgcggc cctgtgtgtt
    16681 gaaacattga ggaatataac cgtgccgtca aattactttc atacgcgtga acgattgggt
    16741 gctttaggcg gcatcgtgca acatcatacc gccttgtacc gcagcgtggc ggtatatgaa
    16801 ccgaccgtgg ttatcgtgcg gcgcggatgc aagaagctgc ggtgggcggg gcgcgaattg
    16861 aggattgcgg cgggtgaggc tgtcggcttg gcaggcgggc agacgcttga cgtcatcaat
    16921 attcccgatt cagacggcct ctatcaggcg caatggattg ctttcgaaca ggaaaccgta
    16981 gaaaggttcg ccgcacaata cgggacggcg caggcagtgt gcgatgcggt gaagctgccg
    17041 caccccgggc gtatgggcgc ggcgtttgat tatgcggctg cggtactggc ggatgaagaa
    17101 gtgccgcaca atgcggcgga ggccgcgctt tgcggcgtgt tggcgtggtt gcagcatgac
    17161 ggcatcggtt ttgccgtgta cggaggcgtc aacctgatgc ggcaaatccg caaactgatt
    17221 actgccgata tggctgcgga ctggtcgtcg gcaatgctgg cgcagcggct taattgcagc
    17281 gaggccgcgt tgcggcggcg gttggcgcgg caggacacga atttccgtac gctgctgacg
    17341 gatgtacgca tgatgcgcgc gctgacgctg ttgcaggtta cgcaatggtc ggtagcgcag
    17401 attgccggcg cggttggcta tgactgcccg tcccgtttca gcgcgcgctt caaagagctg
    17461 ttcggttgcg taccgtcggt ggtccgctcg gaagcggagc cggcggcata tcggcgcacg
    17521 gggcaggtca gcgtcggtgt tcgaccgtga atttcgcttc gcccaagctg tttgccttag
    17581 tggagaaacc gaccagcacg ggtgtggatt cggcggtgat gttcggcagc ttggcgactt
    17641 tcagcgcggt aagcgtgaat tcgtagcggt gtttcctgcc ttcgggagga cacgcgccgc
    17701 cgtagcccgg cgcgccgaag tccgtgcggg tttgcagcgc gcctttgggc ggcctgcctc
    17761 attgtgcggt aatgcctgcg ggcaggcggc ggacatcggc gggaatgtcg gcgaccaccc
    17821 agtgcatccg gtccagaccg atcggcgcgt ctttatcgta aacggtcagg acgaaacttt
    17881 ttgtccctgc gggcgggttt ttccacgaca gcgcgggcga agcattgccg cccgaacagc
    17941 cgaagccgta aggcgcgctc aaaagctggt tttgcgtgaa gccgccgtct tcggacgggt
    18001 tgtcgaattg cagcgtgaat gcgcccccgg caaaggcagc ggttgaaaaa gccagcagta
    18061 cggcggagag gatgcgggtt tgcatagatg attcctttgc ttgatgaata agcggattca
    18121 ttataggctg tcaacggcgg caggaatgtg ccgaaacggt tggatgatgt gccggaatgc
    18181 gcaaatgcaa aaatcgtggt ttcagacgac cctttgaagg ccgtctgaaa tttttcatag
    18241 ggcgacgctt tatgtgtcgt cctgtgtgtt gaaacattcc tttccctgat gctttggaaa
    18301 tattgtatat ttaggcattg ccgcaaccag accctcaagg tttccgccgc agcctgttcc
    18361 tgtaccggcg tgcgggaatg gcggaaatcg gggatttcgc cgtcaaattt atgataggga
    18421 tacggagcaa acgaatgatt accctgcctt ccttaaacaa tctgccgtca aaatgggatg
    18481 aaatccgcca ttgcctcgaa acccgggttt ttgagtgcgg cgttatgccg tatcccaatc
    18541 tgacggaatg cgaagccggg cagttcgagc gggatttttg ggacaatatc ggctgcgcgc
    18601 ctgaagaata tgtccgtatc cgccgcgcca tccgtctgtt ggaattacgt tatcccgaca
    18661 gcttgaacga gctggtgtgt gccgccattg ccacgccttt gggcgagatg ttggcggtgt
    18721 tcggcgttaa gggattgtgc ctgctggagt ttgtcggaca gaagtatttg gaacaggaaa
    18781 ttgccgccgt ccaaaaagcc ttgcgcggac ggtttgtgtt tcgggaagac gggcggatgc
    18841 aatttttgcg tcaggaattg gatttgtatt tccaatgccg tctgaaaacc tttgccacgc
    18901 ccttggaaat gattggtacg gcgtttcaaa aacaggcgtg ggacgtgctt ttggcgattc
    18961 cttacggcga aacgcgcagt tataaggagc aggcgcagcg tttgggcaac cccaaagccg
    19021 tccgcgccgt tgccgccgcc aacgggcaga acaaggtgtc tgtcatgatt ccctgccacc
    19081 gcgtcattgg cagcgacggc aaactgaccg gctacgcggg cgggttgaac cgcaaacagt
    19141 ttttactggc tttggaacgc ggcgaagttc agacggcatt gttttaagcg gggaaatgca
    19201 gaacggctca tcgggcttaa atatggtgtg atgacggctg tgaaaattca tacaacggca
    19261 atccttttaa ggcatcggtc ttaatgtctt tctgattcat atgcttgtcg ttttgtcgcc
    19321 catttttcca aaattaagga ttgacacatt ttttccggac gctataatac gccccattcc
    19381 ccgatagctc agtcggtaga gcgacggact gttaatccgc aggtccctgg ttcgagccca
    19441 ggtcggggag ccagaatttt gaagcccgaa cttgaatgtt cgggcttttt cacatttgca
    19501 cgtccggcgt tcggaatgcg gcgagatagg ttttccaacg gtttcgcggt aaaattctgc
    19561 ctgtctgatt tcggtttcgt tttatgtcta tcaaaatcct gattatttct cccagttgga
    19621 taggtgactg cgtgatgacc cagcccttgt tccgccgttt gaaggaactt catcccggtt
    19681 gcacgattga tgtgttcgca ccgaagtggt cgatggcggt gttcgagcgt atgccggaag
    19741 tgaatgagat tcttgaaaat ccgttcggac acggtgcgtt ggagctgaaa cgccgttggc
    19801 gggtaggcag ggagttggga cggcgcggat acgacagggt tatcgtgttg ccgggttctt
    19861 tgaagtcggc agtcatcgcg ctggcaacgg gcatcggaaa aagaacgggt tatgtcggcg
    19921 aaagccgtta ttttctgttg aacgatatac gcaggctgga taaggaacgt ctgcctttga
    19981 tggtggaccg atatacggct ctcgcgcatc agagtcagga agattttgac gggcattcgg
    20041 gattccccga gttttccatt gatgaacggc ggcgggaaat ttctattgaa actttcggtt
    20101 tgaatcttgg aaagcctgtt ctggcttttt gcccgggtgc ggaattcggg ccggcaaagc
    20161 gttggccggc aaggcatttt gccgagttgg gcaaacacta ttcggaggcg ggttggcagg
    20221 tttggttgtt cggttcgcaa aaagatgatg aaatcgccga agaaatcaac tgcctttcag
    20281 acggcatgtg tgtcaatttg tgcggcaaaa ccgatttgtc gcaggcaatg gatttgctgt
    20341 cgttggcgga cacggtcgtg tgcaacgaca gcgggctgat gcatttggcg gctgctttgg
    20401 gcaggaaggt agtggcggtt tacggttctt ccagcccgac gcatacgccg cctttgagcg
    20461 acagggcgaa aatcgtcagc ctgcacttgg aatgttcgcc ctgtttcaaa cgtgaatgcc
    20521 cgttggggca taccgactgc ctcaacaggc tgtatcccga gaagattgtg caggcggttg
    20581 aagaggcggt atgatggctt ctgttttcgg atttataccg tctgacggcg gatattgata
    20641 ggatgtttat ggctattgcg aacaataaaa aagcatttca cgattttttc attgaagacc
    20701 ggattgaagc cggtttggtc ttggaaggct gggaagtcaa agcaatccgc gccgcgcgcg
    20761 tacagcttaa agagagttat atctattgga aaaaagatgc gttttatctg gtcggctgcc
    20821 atattacggc tttgcctacg gcttcgacac acatcaaacc ggatgccgta cgtccgcgca
    20881 aactgttatt aaaacagtcg gaaatcaaca agttaatcgg aaaaaccgaa cgtgcaggtt
    20941 acaccatcgt gccgctggat ttgcattttt cacgcggaaa aatcaagatg gaaatcggtt
    21001 tggcaaaagg taagaaacag cacgataaac gacaaagtat gaaggaagcc gactggaaac
    21061 gcgagaaaca gcggttgatt aagcatacgc gctgattgtg gaaacaatgc ttcagacggt
    21121 acgggcaagg ctgccgcctg atgcgtaaac gttctacact taaaccagca tccgctttaa
    21181 tcagcacagc atgatgcccc aaaccttcgt caaaggtttg agcaatttga tgataaattg
    21241 gcattagcca agggtaaagt gcggtcattt ttcctgaaat ttttgtaaat aacgagggca
    21301 attatacaca ttttcaaacc ggcttgacaa aatagcaaaa agcaaacgtt tacttttaat
    21361 aaataagaaa taaatagaga aaatttgaac taaatgacat tttttgttca gttttatgct
    21421 aaaatccacg ccctatttat ttttacttga taaaggaaaa tttattatga aactcgaagc
    21481 aagcagaagt ttaaaaaatc atttattata agtctatttt tttctattct ttatacctct
    21541 ccgcttttgg ctgttgatta cgtttacgac aattctaaat taacaaatga gcaaattgat
    21601 cgcttaaaaa aaacttagag ataaaagcag cgaatactgg aaacaagaaa cttatctttt
    21661 aaaaccgacg aatggtagct ctcagaactc tctcaaagtt cccgcactat cctctaaaac
    21721 acaccaattc gaaaacatca acaactcaaa aaagatttct ttttatgaca aggaatacac
    21781 tgaagattac cttgttggct tcgcccgagg tttcggggtt gaaaaaagaa atggggaaga
    21841 agaaaaacca ctccggcaat attttaagga ctgtgtaaat actgagaatt ccaataatga
    21901 taagtgtgaa caaccatcct taggaagcta tgccccgata cgcataaaga gcgatatttt
    21961 cgccttagca tcacaaataa aaaatagcca tatcaacagc gaaattttat ctgtaggtaa
    22021 ttatatcgaa tggcttcgct tcgcccaaca cttaatcagc tttctagttg gcaagagcat
    22081 ctctattctg atgtagatcc attccactat attgaggtta cagacaactc gcacgttatc
    22141 gggcaaacga ttagtttgga tgaattccac ttagaaaatt ctctctggga accccgttgg
    22201 gattctgatg ttggggagtt aaaaacaacg aatgcagaca tccgttttaa tactaaaagt
    22261 gaaagtttac tcgtaaaaga aaattatgct ggcggagctc gttttcgttt tgcttacgat
    22321 ctaaaagata aagtccctga aataccagtt ttaacttttg aaaaaaatat aactggcaca
    22381 tcagatatta tttttgaggg aaaagcgttg gataatttga aacacctaga cgggcatcaa
    22441 attgtcaaag taaatgacac cgcagataaa gatgccttta gactttccag taaataccga
    22501 aagggaattt atacgctctc tttacaacaa cgcccagagg gcttttttac caaagtgcaa
    22561 gaacgcgatg atattgcgat ttatgcacaa caggctcaag ccgccaatac cttattcgcc
    22621 ttgcgtttga acgacaaaaa cagcaatatt tttgaccgca ctttaccacg caaaggcttg
    22681 tggttgcgtg tgattgacgg acattccaac caatgggtgc aaggcaaaac agcacctgta
    22741 gaaggctatc gaaaaggtgt gcaactcggc ggcgaggtgt tcacatggca aaacgaaagt
    22801 aatcaacttt ctattggctt aatgggcgga caagcagaac aacgcagcac tttccgcaat
    22861 ccggatacag acaatcttac aacgggaaat gtgaaaggct ttggtgcggg cgtttacgcc
    22921 acttggcatc agcttcagga caaacagaca ggtgcgtatg ttgatagctg gatgcaatat
    22981 caacgtttcc gccaccgcat taacactgaa tatgctacag aacgttttac ttcaaaaggt
    23041 attactgcct caattgaagc gggtcacaat gcgttattgg cggaacactt cactaaaaag
    23101 ggcaacagcc ttcgtgttta cctacaacca caggcgcaat tgacttattt gggggtaaac
    23161 ggaaaattca gcgatagcga aaatgcccaa gtgaatttgc ttggctcccg ccaattacaa
    23221 agccgagtgg gcgttcaagc taaagcgcaa tttgctttca ctaatggcgt cactttccaa
    23281 ccatttgttg ctgtcaattc aatctaccaa caaaaacctt tcggtgtgga aatagacggg
    23341 gatcgtcgag tgataaacaa taagacggta attgaaactc aattaggtgt agcagcgaaa
    23401 atcaaatctc acttaacctt gcaagcctca ttcaaccgcc aaacaagcaa acatcatcac
    23461 gctaaacaag gtgcgttgaa tttacagtgg acgttttaag ttaaattgta acaaaataaa
    23521 atcggcaggg aaattcactg ccgattttat tattttaaaa gtgcggtcat ttttcgtgta
    23581 atttccacca atttttaccg cactttcaat ttcagcttga acaagttcaa tagtctgctc
    23641 tgcattaatt acgactgctt ttggattatc ttttactaat tctaaatagc gtgctcgagt
    23701 gcgatggaaa aaatctaaat ccatttgctc aatacggtct aactcgccac gtccacgagc
    23761 tcgcgctaaa ccgacaatcg gatctattgt ccttttcttt gttgtgtatg caaatcagaa
    23821 atgccggtta cggaagcagt ttgccaggat tcataatgtt atatggatca aggtgttgtt
    23881 tgatgctttt catcagggca atttcagagg gcgtgcggac agcgggcagc cattgttttt
    23941 tgatgatacc tatgccgtgt tcggcggcaa tcgtgccgtt gcgggcaagg acgttgcgat
    24001 agactgtgct gttgatgtcg ttttcgtaac ggtaaacttc attgctgagg atttcgggcg
    24061 gaaaagtatt gtagtgcagg ctgccgtcgc ccagatgtcc gaagcagacg atttgtatgc
    24121 ctttaaaatt ctgttccaaa tctttggcgc accggcggac aaagtcggca acgcgcccga
    24181 taggaacggc aatatcgtgt ttgatgctgg tgcccagttt gcgttgggag gcggagatgt
    24241 tttcgcgcaa cgcccacata tggatacgtt cttgttcgct ttgcgccaat acgctgtcgg
    24301 taaatccttt tttataaaga aattcgacaa gccgatcatc aagattgctg tcgggtaatg
    24361 agtcggtcaa ctcaagtaaa atatgccatt ctgaatgtgt cggcaggggg agtttgctga
    24421 attcggaaga caactcggcg gcaaaacggc cgatcagttc aaaactgcat aagcgttcgg
    24481 caaagtgcgc ttgggtttcg gtcagcaggc ggacggcgga ttcgatgtcg ggaatgccga
    24541 cccatgccgt tgccttgtcc gagggacggg caaaaagctt gagcgtggcg gcagtgatga
    24601 tgcccagtgt gccttcgctg ccgataaaca gatggcgcag gtcgtagccg gtggtgtttt
    24661 tatgcagggg atggagatgg gaaaccagtt cgccgttggg gaggacgact tccaaaccga
    24721 taaccaggtc gcgcatcgtg ccgtaacgca atacgttcaa acctccggca ttgcaggcga
    24781 tgttgccgcc gatttggcac gagccttcgc tggcgagact gagtgggaac agcctgtttg
    24841 aggcttcggc tgcctgttgg acggtttgga gtacggaacc tgcttcgacg gttatgcagt
    24901 tgtctgacaa attgatgctg cggatgcggt tgagtttgga aaggttcagc aatacgccgt
    24961 tttccgatac tgccgcgccg cacaaaccgg tattgccgcc ttgcggcgta accggaatgc
    25021 ggtgttcgta acaaaaacgc ataatggttt gcacgctttc aacgctgcgc ggctgcaaaa
    25081 tgatgtctgg tgcagacgta aagcggttgc gctgatcttt taaaagcgtc ggagaaggtt
    25141 cggcaatttc atctgccggc aggagtcggg aaaattcggt atgaaggtca ggcatgtttg
    25201 tcggaagggt agggagaaga ttgaggaagg gaatctgatg ccgtctgaaa aatcaaaaaa
    25261 gcaggatact gaacgtatcc tgcttttttg caagcggaaa atgcttattt tgcagcttcg
    25321 gcagcaggag cttcagcggc aggtgcttcg gtagcagcag cttcggcggc aggagcttca
    25381 gcagcgggag cttcggcggc aggtgcttcg gcggcgggag cttcggcggc aggtg
//
LOCUS       DAWTQS010000023        24887 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.023, whole genome shotgun sequence.
ACCESSION   DAWTQS010000023 DAWTQS010000000
VERSION     DAWTQS010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 24887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24887
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.023"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(59..253)
                     /locus_tag="WNA25_000973"
     CDS             complement(59..253)
                     /locus_tag="WNA25_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFA9823744.1"
                     /translation="MKHKLASWWKTIKLTANLMAGVPDYENYVAQQRKHNPNAPVMNK
                     LQFQDYCRKRRCGANGGRCC"
     gene            complement(243..2324)
                     /locus_tag="WNA25_000974"
     CDS             complement(243..2324)
                     /locus_tag="WNA25_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HFA9823745.1"
                     /translation="MKSLKTFLIWGIVVLVGLASFTTLALSRGEQVSAVWMVTAAISV
                     YCIAYRFYSLYIANRVMRLDPNRLTPAERHNDGLDYVPTHKGVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGTLWIIFGVVFAGAVQDMTVLFVSMRRDGKSLGDIVKQELGTVPG
                     VIASIGILMIMVIIMAVLALIVVKALVHSPWGTFTIAATMPIALFMGIYTRHIRPGKI
                     GEISIVGFILLMLAVIYGEDVAKSSIGHWFDLDGIQLTWAIMIYGFVASVLPVWLLLT
                     PRDYLSTFLKIGTIAALALGIVIVNPALQMPAVTHFIDGSGPVFSGALFPFLFITIAC
                     GAVSGFHALISSGTTPKMLENETHVRMIGYGGMLMESFVAIMALAAAASLDPGVYFAM
                     NSPAALIGTDANTAAEVITTKLNFPVDAATLLHTAKEVGENTILSRTGGAPTLAVGMA
                     HIMSRLIPGEAMMAFWYHFALLFEALFILTAVDAGTRVARFMIQDLGSIFYKPFGNTD
                     SIPANLIATFFAVALWGYFLYTGVTDPLGGINSLWPLFGIANQMLAGVALIMCAVVLI
                     KMKRDRYVWVALVPAVGVLLVTCYAGLQKLFHNDPRASFLAHAGKYSDALAKNEVLAP
                     AKDIGEMAQIIFNDKINAGLTILFLSVVVTVAAYGLRTALKARKVGWPTAKEIPAVYR
                     DGKQPEEQSEA"
     gene            2847..3227
                     /locus_tag="WNA25_000975"
     CDS             2847..3227
                     /locus_tag="WNA25_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HFA9823746.1"
                     /translation="MNIRFFALTVSVLSLAACAVPEAYDGGGRGYMPPVQNQAGPDDF
                     RAFSCENGLSVRVRNLDGGKIALRLDGRRAVLSSDVAASGERYTAEHGLFGNGTEWHQ
                     KGGEAFFGFTDAYGNSVETSCRAR"
     gene            complement(3363..3835)
                     /locus_tag="WNA25_000976"
                     /pseudo
     CDS             complement(3363..3835)
                     /locus_tag="WNA25_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244189.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CdiI family contact-dependent growth inhibition
                     immunity protein"
     gene            4183..5571
                     /locus_tag="WNA25_000977"
     CDS             4183..5571
                     /locus_tag="WNA25_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFA9823747.1"
                     /translation="MNEYSQLIKHPDISLSPISDGIGVGNPATGEILAYVRNTGSDKL
                     KNLIQKAAAAQKLWAAKTALERADILWRWYFLVKENKEALARLMTMEQGKSLTEARGE
                     IDYAASFVRWFAEEARRIDGDVLTSVKASQKLVVLKQPVGVTAAITPWNFPSAMIVKP
                     ASLTPLSAYALAVLAYEAGVPQDLLPVVSGRASEISHEFATNPTVRKISFTGSTEVGA
                     KIFAGSAADIKKLSLELGGNAPFIVFDDADLDKAVEGALASKFRNSGQTCVCTNRVYV
                     QSGIYDVFCRKLSEKAAALKLGDGLDEGVNQGPLIEEKAVEKVEQHIADALSKGAVCL
                     TGGKRSALGGTFFEPTVLSGVTAQMAVAREETFGPLCPVFRFETEAEVIEAANNTEYG
                     LAAYLFTSDTARQWRVGEVLEYGMVGINTGLISNEAAPFGGVKRSGLGREGSKYGADE
                     YLELKYLCIDAG"
     gene            5682..6467
                     /locus_tag="WNA25_000978"
     CDS             5682..6467
                     /locus_tag="WNA25_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimb protein"
                     /protein_id="HFA9823748.1"
                     /translation="MMENGKTFPRWRFALKSAGWHLLISLLVAGLAALLVFKVWYPYP
                     YAELTGGLSLYQLVVAVDIVCGPLLTLILASPKKKTKARMVDFSMVGIIQLAALAYGL
                     HSVSLARPVVEAFEQDRMTIVTAAEVVVEDLHKAPEGLQSLSWFGIRRIALKEPEDAD
                     EKNKTLDLSLKGIEPSMRPDRWLPYSDKEAEKIRKHLKPLKVLADARKTTVADILKQA
                     GLAEGEELYYLPFTSSRQKEWIVITDKEGNTKGYAPIDGFIIP"
     gene            6835..8391
                     /locus_tag="WNA25_000979"
     CDS             6835..8391
                     /locus_tag="WNA25_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFA9823749.1"
                     /translation="MDFSWLAEPHTWIGFATLLVLEVVLGIDNLVFVAILANKVQPAR
                     RDRARIIGLGLAVVIRIIMLAFMAHIITLTEPLFQIGGLAVSGKDMIMLAGGIFLLYK
                     ATTELHERLEGHNRFTVADNQKKHAPFWGVVAQILILDAVFSIDSVITAVAMVDHIVV
                     AMGAVVVAMVVMISASKLLTEFVDRHPTVVMLCLGFLLMIGFSLIAEAFHFHIPKGYL
                     YAAIGFSILIELFNQISQRNSRKNDYIGSSWRKRTAENVLGMMGIRESVLADAGGESV
                     DDAHFEENEKSMIRSVLTLAERPIMGVMIPRRDIERLDISQSREEQYAQLQNTPYSRL
                     LVVGKAGVDEPLGYINKKDLLSQLLETGGLDIQTALRQPLVLPDGTTALGALELFRQS
                     SADYALVVDEFGAVLGMVTMKDLLEAIAGEFPEEFEREEEPAVQGNPDESLTVEGALE
                     YVELASQLNLPQQEEDTDFHTVAGLIMEELQTIPDVGDFADFHGWRFEVVEKEGQRIE
                     RVKITKLPEE"
     gene            8408..9154
                     /gene="surE"
                     /locus_tag="WNA25_000980"
     CDS             8408..9154
                     /gene="surE"
                     /locus_tag="WNA25_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFA9823750.1"
                     /translation="MNVLISNDDGYLAEGIAILARVASEFANVRVVAPERDRSGVSNS
                     LTLDRPLQLKQAQNGFCYVNGTPTDCIHVGQFALPDFKPDVVFSGINRGANMGDDTLY
                     SGTVAAATEAYLMGMPAVAFSLNDASGRYWATAEKALWTLLAHFFKKPPSAPVLWNVN
                     IPAVAPEDVRGIKITRLGRRHHEQNIVPSRNPRGEQIYWIGPVGEVSDREEGTDFGEC
                     GAGFITVTPLQIDLTAYPDMAETAAFWHTD"
     gene            9256..10269
                     /locus_tag="WNA25_000981"
     CDS             9256..10269
                     /locus_tag="WNA25_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HFA9823751.1"
                     /translation="MLKQTTLLAACTAVAALLGGCATQQPAPVIAGNSGMQTVSSAPV
                     YNPYGATPYNAAPAANDAPYVPPVQTAPVYSPPAYVPPSAPAVSGTYVPSYAPVDINA
                     ATHTIVRGDTVYNISKRYHISQDDFRAWNGMTDNTLSIGQIVKVKPAGYAAPKTAAVE
                     SRPAVPAAAQTPVKPAAQPPVQSAPQPAAPAAENKAVPAPAPAPQSPAASPSGTRSVG
                     GIVWQRPTQGKVVADFGGGNKGVDIAGNAGQPVLAAADGKVVYAGSGLRGYGNLVIIQ
                     HNSSFLTAYGHNQKLLVGEGQQVKRGQQVALMGNTDASRTQLHFEVRQNGKPVNPNSY
                     IAF"
     gene            complement(10667..11149)
                     /locus_tag="WNA25_000982"
     CDS             complement(10667..11149)
                     /locus_tag="WNA25_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFA9823752.1"
                     /translation="MTQQRQLPSHELIMSELMMPDTANFSGNVHGGELLLLLDQVAYS
                     CASRYSGNYCVTLSVDKVLFKEPIHIGDLVTFYAAVNYTGRTSMEIGIRVEAQNIRTG
                     EIRHTNSCYFTMVAVKDGKPVPVPPLEILTDRQRCRYEKAKKRREISLQASGDVSCGC
                     "
     gene            complement(11302..11463)
                     /locus_tag="WNA25_000983"
     CDS             complement(11302..11463)
                     /locus_tag="WNA25_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823753.1"
                     /translation="MSEQPEKHHASPIEDERKKPVYRMGRAVAGFMLAVWAGVLALVF
                     FLVFRFWLS"
     gene            complement(11537..11998)
                     /gene="recX"
                     /locus_tag="WNA25_000984"
     CDS             complement(11537..11998)
                     /gene="recX"
                     /locus_tag="WNA25_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFA9823754.1"
                     /translation="MKPQKSLRARAMDILSRQEVSRIGLKRKLAPHAESEEELENVLN
                     EFAERNWQSDLRYAEAYIRSKSRKHGSLRLKQALAQQGIDEKTSRNLLPDRSSEKQAA
                     IAVLRKKFKHPAANLKEKQKQARFLAYRGFDADTVQTALKHAWDENWEDSC"
     gene            complement(12071..12781)
                     /locus_tag="WNA25_000985"
     CDS             complement(12071..12781)
                     /locus_tag="WNA25_000985"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFA9823755.1"
                     /translation="MNAAIEHVQAVAFDLDGTLCDSVPDLAAAAEAMLEQLGMKPLPA
                     KVVESYVGDGIGKLVHRVLTNDRDREADSELWEKGFVSYMKYYRDHLSVFTRPYPETE
                     AGLALLKSLGIPLVIITNKNEILAAELLKQLGLADYFSLILGGDSLPEKKPSPLPLRH
                     AAEVLGIDAANMLMVGDSRNDIIAAKAAGCLSVGVTFGYGDMTLLSQDDTTRPDRIIG
                     ALPEIYENLRPQKNKDEE"
     gene            13176..14395
                     /locus_tag="WNA25_000986"
                     /pseudo
     CDS             13176..14395
                     /locus_tag="WNA25_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581011.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
     gene            complement(14455..15261)
                     /locus_tag="WNA25_000987"
     CDS             complement(14455..15261)
                     /locus_tag="WNA25_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HFA9823756.1"
                     /translation="MKKTLTLLAVSALFATSAHAHRVWVETAHTHGGEYLKADLGYGE
                     FPELEPIAKDRLHIFSKPMQLVTEKGKENMIQRGTYNYQYRSNRPVKDGSYLVTAEYQ
                     PTFRSKNKAGWKQAGIKEMPDASYCEQTRMFGKNIVNVGHESADTAIITKPVGQNLEI
                     VPLDNPANIHVGERFKVRVLFRGEPLPNATVTATFDGFDTSDRSKTHKTEAQAFSDTT
                     DGKGEVDIIPLRQGFWKASVEYKADFPDQSLCQKQANYTTLTFQIGHSHH"
     gene            15453..15662
                     /locus_tag="WNA25_000988"
     CDS             15453..15662
                     /locus_tag="WNA25_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HFA9823757.1"
                     /translation="MPYVNIKVTGGKEAPTAAQKAELIGGVIELLARVLGKNPETTVV
                     VIDEVDTDNWDIGGKSVSERRKEGR"
     gene            15751..16965
                     /locus_tag="WNA25_000989"
     CDS             15751..16965
                     /locus_tag="WNA25_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFA9823758.1"
                     /translation="MDKFPKSAKLDHVCYDIRGPVHKKALQLEEEGNKILKLNIGNPA
                     PFGFEAPDEILVDVIRNLPTSQGYCDSKGLYSARKAIVHYYQNKGLRDITVNDVYIGN
                     GVSELIAMSMQALLNDGDEILIPAPDYPLWTAAATLAGGTVRHYLCDEENGWFPNLAD
                     MEAKITSKTKAIVVINPNNPTGAVYSKEILLEIAELARKHGLIIFADEIYDKILYDGA
                     VHCHIAALAPDLLTVTFNGLSKAYRVAGFRQGWMVLNGPKHHAKGYIEGLDMLSSMRL
                     CANTPMQHAIQTALGGYQSINEFILPGGRLLEQRNRAWELVNQIPGVSCVKPMGAMYM
                     FPKIDTEMYRIRDDMKFVYDLLVREKVLLVQGTGFNWIKPDHFRIVTLPYVHQIEEAM
                     GRLARFLQTYHQ"
     gene            complement(17037..19616)
                     /gene="clpB"
                     /locus_tag="WNA25_000990"
     CDS             complement(17037..19616)
                     /gene="clpB"
                     /locus_tag="WNA25_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFA9823759.1"
                     /translation="MRYDKLTAKFQQALAEAQSLALAADSSYLEAGFVLKALLDDQNS
                     GAAALLAHAGVNVPQVKQRLQQHLNSLPKVSGQGGEILPSRELQAVLNLMDKAATKRG
                     DAYIASELFLLALVQQNDAAGKILKEAGATEQNINAAIDAVRGGQNVNDANAEDQRDA
                     LKKYTLDLTQRARDGKLDPVIGRDDEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPESLRNKRLLVLDLAALIAGAKYRGEFEERLKGVLNDLAKDDGNTLIF
                     IDEIHTLVGAGKTDGAMDAGNMLKPALARGELHCIGATTLDEYRQYIEKDAALERRFQ
                     KVLVGEPSVEDTIAILRGLQERYEIHHGIDITDPAIVAAAELSDRYITDRFLPDKAID
                     LIDEAASRVKMEKETKPEAMDKIDRRLIQLRMEKAHVEKEKDDASKKRLELIDEEIDG
                     LQKEYADLDEIWKAEKAISDGAANIKKQIDEVKIKIEQAKRQGDLALASKLMYEDLEH
                     LEKQRAAAERADTDSTKPANKLLRNNVGADEVAEIVSRMTGIPVSKMMEGERDKLLKM
                     EEVLHRRVVGQNEAVRAVSDAIRRSRSGLADPNKPYGSFLFLGPTGVGKTELCKALAG
                     FLFDSEDHLIRIDMSEYMEKHAVARLIGAPPGYVGYEEGGYLTEQVRRKPYSVILLDE
                     VEKAHPDVFNILLQVLDDGRLTDGQGRTVDFKNTVIVMTSNIGSQHIQQMGTQDYEAV
                     KEVVMEDVKEHFRPEMINRIDEVVVFHGLDQANIRSIAKIQLKGLEKRLEKQNLRLTV
                     SDAALDIIAKAGFDPIYGARPLKRAIQSEIENPLAKALLAGNYAPESEIKVEADGDRL
                     KFA"
     gene            19876..20886
                     /gene="trpS"
                     /locus_tag="WNA25_000991"
     CDS             19876..20886
                     /gene="trpS"
                     /locus_tag="WNA25_000991"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFA9823760.1"
                     /translation="MSKKRVLTGVTTTGTPHLGNYVGAIRPAVRAAQNPDTESFLFLA
                     DYHGIIKCHEQEMIHQSTQAVAATWLACGLDPERTTFYRQSDIPEVMELNWILTCITA
                     KGLMNRAHAYKAAVQANAENGQEDPDFGVEMGLFSYPILMTADILMFNANEVPVGRDQ
                     IQHVEMARDIAGRFNHRFQELFTLPEVKIDENVELLVGLDGRKMSKSYGNTIPLWEND
                     KKTQKSVNKIITNMKEPGEPKQPDESPLFEIYKAFSTPSETAEFTQMLADGLAWGEAK
                     KLSAAKINAELAELRERYNALTSNPSQIEEILQAGAQKARKEARELLDKVRDAVGIRP
                     LK"
     gene            20967..21512
                     /locus_tag="WNA25_000992"
     CDS             20967..21512
                     /locus_tag="WNA25_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823761.1"
                     /translation="MLKIPFAVLGGCLLLAACGKSENTAEQPQNAAQSAPKPVFKVKY
                     IDNTAIAGLALGQSSEGKTNDGKKQISYPIKGLPEQNAVRLTGKHPNDLEAVVGKCME
                     TDGKDAPSGWAENGVCHTLFAKLVGNIAEDGGKLTDYLISHSALQPYQAGKSGYAAVQ
                     NGRYVLEIDSEGAFYFRRRHY"
     gene            21829..22173
                     /locus_tag="WNA25_000993"
     CDS             21829..22173
                     /locus_tag="WNA25_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823762.1"
                     /translation="MKKLLIAAMMAAALAACSQEAKQEVKEAAQAVESDVKDTAASAA
                     ESAASAVEEAKGQVKDAAADAKASAEEAVTEAKDAAAETKEAVSEAAKDTLNKAADAA
                     QEAADKMKDAAK"
     gene            complement(22383..23891)
                     /locus_tag="WNA25_000994"
     CDS             complement(22383..23891)
                     /locus_tag="WNA25_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HFA9823763.1"
                     /translation="MTTNPANVLASVDLGSNSFRLQICENNNGQLKVIDSFKQMVRFA
                     AGLDEQKNLSTSSQEQALDCLAKFGERLRGFRPEQVRAVATNTFRVAKNIADFLPKAE
                     AALGFPIEIIAGREEARLIYTGVIHTLPPCGGKMLVIDIGGSSTEFVIGSTLNPDITE
                     SLPLGCVTYSLRFFQNKITAKDFQAAISAARNEIQRISKNMRREGWDFAVGTSGSAKS
                     IRDVLAAEMPQEADITYKGMRALAERIIEAGSVKKAKFENLKPERIEVFAGGLAVMMA
                     AFEEMKLDRMTVTEAALRDGVFYDLIGRGLNEDMRGQTVAEFQHRYHVSLNQAKRTAE
                     TAQTFMDSLCHAKNVTVQELALWQQYLGRAAALHEIGLDIAHTGYHKHSAYILENADM
                     PGFSRKEQTILAQLVIGHRGDMKKMGGIISGNEMLWYAVLSLRLAALFCRSRQDLGFP
                     KNMQLRTDTESCGFILRIDRKWLERHPLIADALEYESVQWQKINMPFKVEAV"
     gene            complement(24054..24785)
                     /locus_tag="WNA25_000995"
     CDS             complement(24054..24785)
                     /locus_tag="WNA25_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HFA9823764.1"
                     /translation="MSREINAGGHHFLYGGISGGGVHYWDNKDFSEQCLHLSLGYKNR
                     SVTRSFGIVPFVGQNLLGGSRYNFVGGFNADFSRRLGERWRLTLNAGNMWKHYQEDRT
                     AARYGSHMPLAGATLMYSAPKDRLLYGGADWPHDMTKEAEQASVRKGLRVGAVKTFDG
                     GLGLRANLRYTRRTFDAPGTIVYRFPRKDHEYQANLSLRHDKISWKGFTPQLNFRYLK
                     IDSNMKSFYTRKNTQIFMSVEKDFK"
BASE COUNT         5691 a         6270 c         6898 g         6028 t
ORIGIN      
        1 ccttattata aaaataccgt ctgaagtggc gttcagacgg catttgtttc aagcggggct
       61 aacagcagcg tccgccgttt gcgccgcagc ggcgtttgcg gcaatagtct tgaaattgca
      121 gcttgttcat cacgggggcg ttgggattat gtttacgttg ttgtgcaacg tagttttcat
      181 aatcgggcac gcctgccatc aagtttgccg tcagcttgat ggttttccac caagacgcga
      241 gcttatgctt cactttgttc ctccggttgt ttgccgtcgc ggtacaccgc cgggatttct
      301 ttggcggtcg gccagccgac tttgcgtgct ttgagggcgg tacgcaaacc gtacgcggca
      361 acagtcacga caaccgacaa gaagaggatg gtcagaccgg cattaatctt gtcgttgaag
      421 atgatttgcg ccatttcgcc gatgtcttta gcgggcgcaa ggacttcgtt tttagccaat
      481 gcgtcgctgt atttgcctgc gtgggcaagg aagctggcgc gcgggtcgtt gtggaacagt
      541 ttttgcagac cggcgtagca ggttacaagc agtacgccga cggcaggaac gagtgccacc
      601 cagacataac ggtcgcgttt catcttaatc agcacaacgg cacacataat caaggctacg
      661 cctgccagca tttggttggc gatgccgaat aaaggccaga gcgagttgat gccgcccagc
      721 gggtcggtta cgccggtgta gaggaagtag ccccacaatg ccacggcgaa gaaggtcgca
      781 atcaggttgg cggggatgga gtcggtgttg ccgaaaggtt tgtagaagat gctgcccaag
      841 tcttgaatca taaaacgtgc gacgcgcgta ccggcatcga cggcggtcag gatgaacaag
      901 gcttcaaaca acagggcgaa gtgataccag aatgccatca tcgcctcgcc cggaatcaga
      961 cggctcataa tgtgcgccat accgactgcg agggtaggcg caccgcctgt gcgggacagg
     1021 atggtgtttt cgcccacttc cttagcggtg tgcaacaggg ttgcggcatc gacagggaag
     1081 ttcagcttgg tggtaattac ttcggcggcg gtgttggcat ccgtaccgat cagtgcggcg
     1141 gggctgttca tcgcgaaata cacgccggga tcgagcgatg cggcggcggc aagcgccata
     1201 atcgccacga aactttccat caacataccg ccgtaaccga tcatacggac gtgggtttcg
     1261 ttttccagca ttttcggcgt ggtgccggaa gaaatcagtg cgtggaagcc cgaaaccgca
     1321 ccgcaggcga tggtaatgaa caagaacggg aacaatgcgc ctgagaatac cgggcccgaa
     1381 ccgtcgataa agtgggtaac cgcaggcatt tgcaaagcgg gattgacgat gacgataccc
     1441 aaagccaagg ccgcaatcgt accgattttc aggaaggtgg agagatagtc gcgcggggtc
     1501 agcaagagcc agaccggcaa aaccgaagcc acgaaaccgt aaatcataat cgcccaagtg
     1561 agctggatgc cgtcaaggtc aaaccaatgc ccgatggagc ttttcgccac atcttcgccg
     1621 taaattaccg ccaacatcag caaaataaag ccgacgatgg aaatctcgcc gattttgccc
     1681 ggacggatat ggcgcgtgta aatacccata aacagcgcaa tcggcatcgt tgcggcaatg
     1741 gtgaacgtac cccaagggct gtgaaccaat gcttttacga caatcaacgc caaaaccgcc
     1801 ataatgatga ccataatcat caaaataccg atggaggcaa tcacgccggg gacagtgccg
     1861 agttcctgtt tcacaatatc gcccaaagac ttaccgtcgc ggcgcataga gacgaacaag
     1921 accgtcatat cctgtaccgc gccggcaaat accacgccga agataatcca caaagtaccg
     1981 ggtagataac ccatttgcgc cgccaaaacc ggaccgacca aagggcccgc gccggcaatc
     2041 gcggcaaagt ggtgtccgaa caatacgcct ttgtgcgtcg gaacgtagtc caagccgtcg
     2101 ttgtgccgct cggcgggcgt gagacggttc ggatcgagcc gcatcacgcg gttggcgatg
     2161 tagaggctgt aaaaacggta ggcgatacag taaacggata tggcggcggt aaccatccat
     2221 accgcgctga cctgttcgcc tcggctgagg gccagagtgg taaaggatgc taagccgacc
     2281 agtaccacta tgccccaaat gaggaaggtt ttgagtgatt tcatcgaata aatccttatc
     2341 tcacactgtc ggaatatgtc ggagcggaca ggcaacggtt ggctacccgt aatccgatgc
     2401 cgacacaaac cggagtttga atgattgacg aagtatgaaa cagtgcgctt gtcgggactt
     2461 gtgccttatg cccgcatcaa acagtgcagg aatgtacgcg cacaaacgtt aaacgccgaa
     2521 tagaatttta acgcaaatta gcacactgat agcggttttt acttggaatt tggaaaaatt
     2581 tacattcctc cgggcgggca ggcaggttca gacggcatcg tcaggcaaaa ggcggtgtcg
     2641 gaagacgggt aaagaagggg cgcgcaatcc ggattattga tttatcgtgt ttccttttcg
     2701 gttgaacctc agccctttgg ggcggtaaaa tcagacttta tttgggaggg gcgcaacccc
     2761 ttccgaatca ggacggcaca tagggcggtg ctttaatgtg tcgtcctgtg tgttgaaaca
     2821 tattttcttt ttattgggga gcttttatga atatcaggtt tttcgcgctg accgtatcgg
     2881 ttttgtcttt ggcggcctgt gccgtgccgg aagcgtatga tggcggcgga cgcgggtata
     2941 tgccgcctgt tcaaaaccaa gccggcccgg acgattttcg agcgttttca tgcgagaacg
     3001 gtttgtctgt gcgcgtccgc aatttggacg gcggcaaaat cgcgttgcgg ctggacggca
     3061 ggcgtgccgt cctctcttcc gacgttgccg catccggcga acgctatacc gccgaacacg
     3121 gtttgttcgg aaacggaacc gagtggcacc agaaaggcgg cgaagccttt ttcggcttta
     3181 ccgatgccta cggcaattcg gtcgaaactt cctgccgcgc ccgttaaacg gggcagcctg
     3241 tttgaaccgc cgtccggatt tttcgggcgg ttttttatgc cggttttgtt ttggccgtcg
     3301 gctggttccg gcggttttcg ggcgtggcgg attatttttc gtttcgttct gggtttatcc
     3361 tgtcggcagc ggctcaatgc caactttaaa cctgctccga tttcttcagg gctgttatcc
     3421 aatgataaaa ttacatcgtc tgcatcaatg gcatcccacg cttccggctt gacatggcgg
     3481 ctcgggctga ttttcaggca gccgttgtgc agccaaatat ccccgctcat catgttttta
     3541 aatagggcgc gtctggtttt atagcccaag ttcccgcata ggttggcaac ccaatcctca
     3601 tagcgttgcc gaaatttttc ggtatcaaaa aaatcttggt cttccgaact gtcataaacg
     3661 aaagtcctgc tgtttgccaa cgcttgcaag accgtcgtgc ctaaagtttc attgtcggta
     3721 tccaatggca ggatatgggg ggatataggt ggtctattgc cgttaacccc aaacctgaac
     3781 atgtttgaac aatcagagct tcttcattag cataaaaaac tgcccaataa ttgatcctgt
     3841 ttaaaaatca ttatttaatc tccgtaattt tgactgtaat gttttgactt ttgccatact
     3901 ctaccacgcg ttgtaactgc aatctttgct tcttatatat tgtaccctat caaagggctg
     3961 cattactttt ctttctaaag attactatcg cataaaaagt atttttctta aacgacaggg
     4021 atgcgcccgt gccatattca aacaccgccc ggatgttgcg ctgcccgatc ggatgcttca
     4081 gacggcatcg gaagggtttg cagttttgga atatgaagat gataatgtcc gcgagattga
     4141 cggcatttga aagcggttat cccgtatacc ggaggatacg aaatgaacga atattcccaa
     4201 ttaatcaagc atcccgatat ttccctttcc ccgatttcag acggcatcgg ggtcggcaat
     4261 ccggcgacgg gcgaaatttt ggcgtatgtc cgcaacacgg gttcggacaa gctgaaaaac
     4321 ctgattcaaa aggcggcggc agcgcaaaaa ttatgggcgg cgaaaaccgc cttggaacgc
     4381 gccgacatat tgtggcgttg gtatttttta gttaaagaaa acaaagaagc actggcgcgt
     4441 ctcatgacga tggagcaggg caaaagcctg accgaggcgc gcggcgaaat cgattatgcg
     4501 gcttcgtttg tgcgctggtt tgccgaagag gcgcggcgga ttgacggcga tgtgctgacg
     4561 agtgtgaaag cgtcgcaaaa actggttgtg ttgaaacagc ccgtcggcgt taccgctgcg
     4621 attacgccgt ggaatttccc gtccgcgatg atagtcaaac ctgcatcgct cacgcctttg
     4681 agtgcgtatg cgttggctgt actggcctac gaagcgggcg tgccgcagga tttgcttccc
     4741 gtcgtcagcg gccgcgcttc ggaaatcagc catgaatttg ccacgaaccc gaccgtgcgc
     4801 aaaatcagct tcaccggctc gaccgaagtc ggcgcgaaaa tttttgccgg cagcgcggcg
     4861 gacattaaaa aactcagttt ggaactgggc ggcaacgcgc cgtttatcgt gtttgacgat
     4921 gccgatttgg acaaagccgt cgaaggcgcg ctggcgagca agttccgcaa cagcggtcag
     4981 acctgcgtct gcaccaaccg cgtttacgtc caatcgggca tttacgacgt attttgccgc
     5041 aaattgagtg aaaaggcagc cgcgctcaaa ttgggcgacg gcttggacga aggtgtgaac
     5101 caaggaccgc tgattgagga aaaagcggta gaaaaagtcg aacagcacat cgccgacgca
     5161 ctctccaaag gcgcggtctg tctgaccggc ggcaaacgca gcgcgttggg cggaacgttt
     5221 tttgagccga ctgtcttaag cggcgttacg gcgcaaatgg cggtagctcg cgaggaaacc
     5281 ttcgggcctt tgtgtccggt gttccgtttt gaaaccgaag ccgaagtcat cgaggctgcg
     5341 aacaatacgg aatacggttt ggcagcttac cttttcacct ccgacaccgc ccgtcaatgg
     5401 cgcgtcggcg aagtcttgga atacggcatg gtcggcatca atacgggctt aatcagtaat
     5461 gaagcggcac cgttcggcgg ggtgaaacgc agcggtttgg gacgtgaagg cagcaaatac
     5521 ggtgcggacg aatatctaga attgaaatat ttgtgcattg atgccgggtg acggatgccg
     5581 tctgaatggc agggtttcag acggtgtcgc attttaagca gtctctatct gttgtacaat
     5641 gcgccctgtt tttacggtta tttttgattt gaatgtaaga tatgatggaa aacggaaaaa
     5701 cattcccgag atggcgtttt gccttgaaaa gtgcgggctg gcacctctta atcagcttat
     5761 tggttgcagg gctggcggcc ttgctggttt ttaaggtttg gtatccttat ccttatgccg
     5821 agctgacggg cggtctgtcg ctttatcagc tggtggtggc ggtcgatatc gtatgcggcc
     5881 cgctgctgac tttaattttg gcaagcccga agaaaaagac aaaggcacgc atggtcgatt
     5941 tttccatggt cggcatcatc cagctggcgg ctttggcgta cggtttgcac agcgtttcgc
     6001 tggcgcgtcc cgtggtggaa gcgtttgaac aggaccgcat gaccattgtt acggcggcgg
     6061 aagtcgtggt cgaagatttg cacaaagccc ccgaagggct gcaaagcctg tcgtggttcg
     6121 gcatccgccg cattgcattg aaagaacctg aggatgcgga tgagaagaac aagacgctgg
     6181 atttgtccct gaaaggtatc gagccgagta tgcgtcccga ccggtggctg ccgtattccg
     6241 acaaggaagc agaaaaaatc cgcaaacatc tgaaaccgct gaaagtcttg gcggatgcga
     6301 gaaaaacgac ggttgcggac attctgaaac aggcaggtct cgccgaaggg gaggagctgt
     6361 attacctgcc gtttaccagc agcaggcaga aagagtggat agtcattacc gataaagagg
     6421 gcaacaccaa aggctacgcg ccgatagacg gcttcatcat cccttaacca ttgggattcc
     6481 gcccgcactc gaacatccgt tcttcgcggc ggtagaatca gactgtattt gagaggggcg
     6541 caacccctca atcagggcaa cacataggga gacgctttat gtgccgtccg tgtattgaaa
     6601 catttcaaaa taagcccatg ccgtccgaac atcctttcag acggcatggc gttttgccat
     6661 tgccccgata agctgttaaa ctgtttaaat tatttcgacc gaaggtacac gcacatgaaa
     6721 gaccccgagc agagcagtaa gcccgcccgc cgttttttgt gcgtaccgtc tgttcaggca
     6781 gcttgagcgt ttcaggctgc ctttttgcgt ttgaacttga ggaacacgaa aatcatggat
     6841 ttcagttggt tggcagaacc gcatacctgg ataggttttg ccacgctttt ggtgttggaa
     6901 gtcgtattgg ggatagacaa ccttgtcttt gtggcgattt tggcaaacaa ggtccagccc
     6961 gcacggcgcg accgcgcacg gattatcggg ctggggctgg cagtcgtcat ccgcatcatt
     7021 atgcttgctt ttatggcgca catcatcacg ctgaccgagc cgctgttcca aatcggcggc
     7081 ctcgccgttt ccggcaagga catgattatg ctcgcgggcg gtattttcct gctttacaaa
     7141 gccaccaccg aactgcatga acgcctcgaa gggcacaacc gttttaccgt tgccgacaac
     7201 caaaaaaaac acgcgccgtt ttggggcgtg gtcgcgcaaa tcctgatact ggatgccgtg
     7261 ttttccatcg attcggtcat caccgccgtg gcgatggtcg atcacatcgt cgtggcgatg
     7321 ggcgcggtcg tcgtcgcaat ggttgtcatg atttccgcca gcaaactctt gaccgaattt
     7381 gtggacaggc atcccaccgt cgtgatgctc tgccttggtt ttttgttgat gatcggtttc
     7441 agcctgattg cggaagcctt ccatttccac attcccaaag gctacctcta cgccgccatc
     7501 ggcttctcga ttttaatcga actgtttaac cagatttcgc aacgcaacag ccgcaaaaac
     7561 gactacatcg gcagctcgtg gcgcaagcgc accgccgaaa acgttttggg tatgatgggc
     7621 atacgcgaaa gcgtgcttgc tgacgcgggc ggcgaatccg tggatgacgc gcattttgaa
     7681 gaaaacgaaa aatcgatgat acgcagcgtg ctgacgcttg ccgaacgccc gattatgggg
     7741 gtgatgattc cacgccgcga catcgaacgg ctggacattt cccaaagccg cgaagaacag
     7801 tatgcccaac tgcaaaacac gccttacagc cgcctgctcg ttgtcggaaa ggcgggcgtg
     7861 gacgaaccgt tgggctacat caacaaaaaa gacctgctgt cccaactgct ggaaacaggc
     7921 ggtctcgaca ttcagacggc attgcgccag ccgctcgtcc tgcccgacgg cacgaccgcc
     7981 ttgggcgcgc tcgaactctt ccgccaaagc agcgcggatt acgctttggt ggtggacgag
     8041 ttcggcgcgg tgttgggtat ggtgacgatg aaagacctgt tggaagccat cgcaggcgag
     8101 ttccccgaag aattcgagcg cgaagaagaa cccgccgttc aggggaatcc cgatgaaagc
     8161 ctgacggtgg aaggcgcgtt ggaatatgtg gagctggcat cccaactcaa cctgccgcag
     8221 caggaggaag ataccgattt ccataccgtt gccgggctga ttatggaaga attgcaaacc
     8281 atccccgatg tcggcgattt tgccgatttt cacggctggc ggtttgaagt ggtcgaaaaa
     8341 gaagggcagc gcatcgagcg ggtcaaaatc accaaattgc ccgaagaata agcatccagg
     8401 ataaaaaatg aacgttttga tttccaacga cgacggctat ctggcagaag gcattgccat
     8461 tctggcgcgg gttgcgtcgg aatttgccaa tgtcagggtg gtcgcgcccg agcgggacag
     8521 gagcggggtc agcaattcgc tgacgctcga ccgtcctttg cagctgaaac aggcgcaaaa
     8581 cggcttctgc tacgtcaacg gcacgccgac cgactgcatc catgtcggcc agtttgccct
     8641 gcccgatttt aagcccgatg tcgtgttttc aggtatcaac cgtggcgcaa atatgggcga
     8701 cgatacgctt tattcgggga cggttgcggc ggcgaccgaa gcctatctga tgggtatgcc
     8761 tgctgtggcg ttttctttaa acgatgcttc cggacgctat tgggcaaccg cagaaaaggc
     8821 actgtggaca ttgttggcgc atttttttaa aaaaccgccg tccgcgcccg tcttgtggaa
     8881 tgtcaacatc cctgctgtcg caccggaaga tgtcagggga atcaagatta cccgtttggg
     8941 cagacggcat catgagcaga acatcgtccc gtcccgcaat ccgcgcggcg aacagattta
     9001 ttggataggg ccggtcggcg aagtttccga tcgggaagag gggacggatt ttggtgaatg
     9061 cggcgcaggt tttattaccg taacgccgct gcaaatcgat ttgaccgcct atccggacat
     9121 ggcggagacg gcggcctttt ggcatacgga ctgaccgttt ttcaaatacc gtcgggtttt
     9181 gcgcttgaga agaacgcgca tttttattat gatataccgc attcaggcta tatatcggaa
     9241 catgaaggtt atcttatgtt gaaacaaacg acacttttgg cagcttgtac cgccgttgcc
     9301 gctctgttgg gcggttgcgc cacccaacag cctgctcctg tcattgcagg caattcaggt
     9361 atgcagaccg tatcgtctgc gccggtttac aatccttatg gcgcaacgcc gtacaatgcc
     9421 gctcctgccg ccaacgatgc gccgtatgtg ccgcccgtgc aaactgcgcc ggtttattcg
     9481 cctcctgctt atgttccgcc gtctgcacct gccgtttcgg gtacatatgt tccttcttac
     9541 gcacccgtcg acatcaacgc ggcgacgcat actattgtgc gtggcgacac ggtgtacaac
     9601 atttccaaac gctaccatat ctctcaagac gatttccgtg cgtggaacgg catgaccgac
     9661 aatacgttga gcatcggtca gattgttaaa gtcaaaccgg caggatatgc cgcaccgaaa
     9721 accgcagccg tagaaagcag gcccgccgta ccggctgccg cgcaaacccc tgtgaaaccc
     9781 gccgcgcaac cgcccgttca gtccgcgccg caacctgccg cgcccgctgc ggaaaataaa
     9841 gcggttcccg cccccgcgcc cgccccgcaa tctcctgccg cttcgccttc cggcacgcgt
     9901 tcggtcggcg gcattgtttg gcagcgtccg acccaaggta aagtggttgc cgatttcggc
     9961 ggcggcaaca agggtgtcga tattgccggc aatgccggac aacccgtttt ggcggcggct
    10021 gacggcaaag tggtttatgc cggttcaggt ttgaggggat acggaaactt ggtcatcatc
    10081 cagcacaatt cctctttcct gaccgcgtac gggcacaacc aaaaattgct ggtcggcgaa
    10141 ggtcagcagg taaaacgcgg tcagcaggtt gctttgatgg gcaataccga tgcttccaga
    10201 acgcagcttc atttcgaggt gcgtcaaaac ggcaaaccgg ttaacccgaa cagctatatc
    10261 gcgttctgac tttccgtttg caatcaggtt aaaaatgccg tctgaaaggt cttcagacgg
    10321 catttttgtt ttggcggctg tgttttcaaa tcattccgaa gggacggggc gtataaattt
    10381 gtttgactgt gtttgggtat ttgcttgatt tcatccgttt gcaatggaaa agccgcttat
    10441 ttccgttcgg agggcggttt tatcggataa aaggcatttt gtccgactga ttagggctgc
    10501 atcaagggaa tatgaaaaca caaccgctta gtctcacggc tgtgttccaa aatggcgttg
    10561 aaaaacggat gagccagaat cagccgattt tacttcaata aatcaaacca ttacaccgaa
    10621 tgtcggaaca atgcttgtcc tgtctttcag acggcatagg ccgccgtcag cagccgcagg
    10681 acacgtctcc ggaggcttgg aggctgattt ctctgcgttt tttggctttt tcgtagcggc
    10741 agcgttggcg gtcggtcagg atttccagcg gagggacggg gacgggtttg ccgtctttga
    10801 ccgcaaccat cgtgaagtag cagctgttgg tatggcggat ttcgccggtg cggatgtttt
    10861 gtgcttcgac acggatgccg atttccatcg aggtgcgtcc cgtgtagttg acagcggcgt
    10921 aaaaggtaac caaatcgccg atgtggatgg gttctttaaa caggactttg tcaaccgaaa
    10981 gggtaacgca ataattgccg ctgtaacggc tggcgcagga ataggcaacc tggtcgagca
    11041 ggagcaggag ttcgccgccg tgtacgttgc cgctgaaatt ggcggtgtcc ggcatcatca
    11101 gttcggacat aatgagttcg tgggaaggca gttggcgttg ttgtgtcatg gtgtacctgt
    11161 tttaagaaag ggtgttttga aatgggtgcg ggaaaatttt agggaatggg gaagtggaat
    11221 tcaagctaaa aaaagctatg gagggatagt ctgccgaaat gggccgccga tgccgtctga
    11281 agccttcaga cggcattttg ttcaggaaag ccaaaaacgg aaaaccaaga aaaacacgag
    11341 tgccagcacg cccgcccaaa cggcgagcat gaatccggca accgcccgtc ccatacggta
    11401 aaccggtttt ttgcgttcgt cttcgatggg ggaggcgtgg tgtttttcgg gttgttcgga
    11461 cattttgttc tcaatcggaa aagttggaaa cgggcgtaag gttacgctgc cgtgcaaaaa
    11521 gatttaagaa ggaaggtcag cagctgtctt cccaattttc gtcccaagcg tgtttcaatg
    11581 ccgtctgaac ggtatcggca tcaaaaccgc gataggcgag gaaacgcgcc tgtttttgtt
    11641 tttctttgag gttggcggcg gggtgtttga attttttacg caacacggct atggcggctt
    11701 gcttttcgct tgagcggtcg ggaagcaggt tgcggctggt tttttcatct atgccctgtt
    11761 gcgccaaagc ctgtttcagc ctcaatgaac cgtgtttgcg gcttttgctg cggatatagg
    11821 cttcggcgta gcgcaaatcc gactgccagt tgcgttcggc aaattcgttt aacacgtttt
    11881 ccaactcctc ttcgctttcg gcgtgcggtg caagtttgcg tttcagaccg atgcggctga
    11941 cttcttggcg cgagaggatg tccatcgcgc gggcgcgtag ggatttttgc ggtttcatgg
    12001 cggcggtttc ggcacggaaa gattcagacg gtatgggaaa cggattccga tgccgtctga
    12061 acagattgtt ttattcttcg tctttgtttt tctgcggtcg caggttttcg taaatttcgg
    12121 gcagcgcgcc gataatccgg tcggggcggg tcgtatcgtc ttgcgagagc agcgtcatat
    12181 cgccgtaacc gaaggtaacg ccgacactca ggcagccggc ggctttggcg gcgatgatgt
    12241 cgttgcgcga gtcgccgacc ataagcatat tggcggcatc gatacccaaa acttcggcgg
    12301 cgtgccgcag cggcagcggg ctgggttttt tttcgggcag gctgtctccg ccgagtatca
    12361 ggctgaagta gtcggcgagt cccagttgtt ttagaagctc ggcggcaagg atttcgtttt
    12421 tgttggtaat gattaccagc ggaatgccca aggatttgag caatgccagc ccggcttcgg
    12481 tttcgggata ggggcgggtg aagacgctca aatggtcgcg gtagtatttc atataggaca
    12541 cgaaaccttt ttcccacagt tcggaatcgg cttcgcggtc gcggtcgttg gtgaggacgc
    12601 ggtgaaccag tttgccgatg ccgtcgccga cgtagctttc gaccactttg gcgggcagcg
    12661 gtttcatacc gagttgttcc aacatcgctt ctgcggcggc ggcaaggtcg gggacggaat
    12721 cgcacagtgt gccgtccaaa tcgaaggcga cggcttggac gtgttcgatg gctgcattca
    12781 taatccggtt tcctgtcggg ttgaaagggt ttattttaac atcgtttgca cagggcttct
    12841 aatgtataga ttattaacaa tcgcaacagg tttttcgggc ggtgttttcg gattttatgg
    12901 cggggtctgg caagggtttg aaatcccggc gatgttccaa ttaaattttt aaaaaataaa
    12961 atgatgacta tatctttatt taattggaca tttgcttttc ggatgcgcgc tgcttttttc
    13021 agacggatgc tgtgcggctt gaaaaataaa atcatatata gattaataaa atcgggctta
    13081 tgtcgactac atgctattac gttttgtcgg gaaattggtt atagtaatga gcgaaatgca
    13141 cgcccctgaa aatctttttg cgaaaaggaa gcaaaatgtc tgatgccgtt gcaaaagaaa
    13201 ccctcaatcc gtttgaaatc gcgcgaaaac aggtcaaaac cgcctgcgac cggctgaaaa
    13261 ccgatccggc cgtttatgaa atcctgaaga gtccgacccg cgtgttggaa gtcaaactcg
    13321 acgacggcac ggtcaaaacc tttaccggtt accgttcgcg tgcgttccca tcccggtgtg
    13381 aatctggacg aagtcaaggc actgtcgatt tggatgacca tcaaatgctg cgttgcaggc
    13441 attccttacg gcggcggcaa aggcggcgtt accttggatc cgcgcgatta ttccgaggcg
    13501 gaactggaac gcatcgcccg agcttatgcc gaagcgattg ccccgctgat cggcgaaaaa
    13561 aatcgatatt cccgcccccg atgtgaacac caacggcaaa attatgtcgt ggatggtgga
    13621 tgcctatgaa aacgtggtga aacattccgc gccgggcgta tttaccggca aaccggttga
    13681 gttcggcggc tctttggcgc gtaccgaggc taccggttac ggcgtgaacc tcgccgccgt
    13741 ccaagctttg gaaaaactgg gcaaagatgt gaaaggcgca acctacgcca ttcaaggttt
    13801 cggcaatgtg ggctaccaca ccggctatta cgcgcatcaa tccggcgcga aagtcgttac
    13861 cgtatctact gttgacgttg ctatctacaa tgaaaacggc ttggatatgg aagcgctgtt
    13921 taaagagttc caagaaaaag gcttcatcac caacaaagcc ggttacggca aagaaattac
    13981 caacgccgaa cttttggctt tggatatgga cgtactcgcc ccttgcgcat tggaaaacca
    14041 gctgacttcc gaaaacgccg gtaaagtccg cgcgaaaatc gtggttgaag gcgcaaacgg
    14101 cccgactacg cccgaagccg acgttatcct gcgtcaaaac ggcgtattgg tcgtgcccga
    14161 tattttggca aactgcggcg gcgtggtcgt ttcctatttc gagtgggtgc aaaacctgca
    14221 aggctattat tgggagtttg acgaagttca ggaaaaagaa atcgtcgtcc tgcgccgcgc
    14281 gttccgcgat atttggaact tggcgcaaga gtatgatgtc gatttgcgta ccgcgtctta
    14341 tatgatgagt atccgccgtg ttgaaaaagc gatgaagctg cgcggctggt attgatgttt
    14401 gaacaaagga tgccgtctga aaccatttca gacggcattt ttgtttggac ggaattaatg
    14461 gtgagaatgg ccgatttgga aggttaaagt tgtgtagttc gcctgttttt ggcacaggct
    14521 ttgatcgggg aaatcggctt tgtattcgac actcgctttc caaaagcctt ggcgcaaggg
    14581 gatgatgtcc acttcgcctt tgccgtcggt ggtgtcggag aaggcttggg cttcggtttt
    14641 gtgcgttttg ctgcggtcgc tggtgtcgaa gccgtcaaat gtagcggtaa cggtggcatt
    14701 gggcagcggt tcgccacgga acagaacgcg gactttgaag cgttcgccta cgtgaatgtt
    14761 ggcgggattg tccagcggga cgatttccaa gttttgtccg accggtttgg tgatgatggc
    14821 ggtgtccgcg ctttcgtgtc ccacgttgac aatgttttta ccgaacatac gggtttgttc
    14881 gcaatagctt gcgtcaggca tttctttgat gccagcctgt ttccagcctg ctttgttttt
    14941 tgaccggaaa gtaggctgat attcggcggt aacgaggtag ctgccgtctt tgacgggacg
    15001 gttgctgcgg tattggtagt tgtatgtgcc gcgttgaatc atgttttcct tacctttttc
    15061 ggtaaccagc tgcatcggtt tgctgaagat gtgcaggcgg tctttggcga tgggttcgag
    15121 ttcggggaat tcgccgtagc ccaagtcggc tttaaggtat tcgccgccgt gcgtgtgggc
    15181 ggtttcgacc cagacgcggt gggcgtgtgc ggatgtggca aatagtgcgg aaacggcgag
    15241 cagtgtcaat gttttcttca tggtgcagcc tttatatcgt gcgggttaaa aattttgtta
    15301 tgctataaca aaatatgtat cgtgtgaaga cggatttgcc gcctgaaaac tgtctttttg
    15361 tatcgatttg aatttttcag aaaatgaagc ggacgtttgg gcggaggcaa cttgtttgat
    15421 aagatagcaa tatttcaaaa cggagaaata tcatgcctta tgtcaatatt aaagtaaccg
    15481 gcggcaagga agcaccgact gccgcgcaaa aagcggaact gatcggcggc gtaatcgaat
    15541 tgctggcacg cgtgctgggt aaaaatcccg aaacaacggt tgtcgtgatt gacgaagtgg
    15601 ataccgataa ctgggatata ggcggcaaaa gcgtcagcga acggcgcaaa gagggcaggt
    15661 aaaaagcctg aaaaccccgc cctgatgctt taaatttcgc gtgaaaaaga gtacattccc
    15721 acccattgcc caaaatttac ggaacacatt atggataaat ttcccaagtc tgcaaagctc
    15781 gaccacgtct gctacgacat acgcgggccg gttcacaaaa aagcccttca gttggaagag
    15841 gagggcaaca aaatcctcaa gctcaatatc ggcaaccctg cgccgttcgg ctttgaagca
    15901 cctgatgaaa tcttagtcga tgtcatccgc aacctgccga cttcgcaagg ctattgcgat
    15961 tcgaaggggt tgtattccgc acgcaaagcc attgttcact actatcagaa caaaggtttg
    16021 cgcgatatta cggtaaacga tgtctatatc ggcaacggcg tgtccgagct gattgcgatg
    16081 tctatgcagg cgttgctcaa cgacggcgac gaaatcctga ttcccgcgcc cgactaccct
    16141 ttgtggacgg cggcggcaac gcttgcgggc ggtacggtac gccattatct gtgcgacgaa
    16201 gaaaacggct ggtttcccaa ccttgccgat atggaagcca aaatcacgtc caaaaccaaa
    16261 gccatcgtcg tcatcaaccc caataacccg acaggagcgg tgtacagcaa ggaaatcctg
    16321 ctggaaattg ccgaactggc gcgcaagcat ggtttgatta ttttcgccga cgagatttac
    16381 gacaaaatcc tttatgatgg cgcggttcac tgccacatcg ccgcgctcgc ccccgacctt
    16441 ttgacggtaa cgttcaacgg tttgtccaaa gcctaccgtg tagccgggtt ccgccaaggc
    16501 tggatggtgc taaacgggcc gaaacatcac gcaaaaggtt atatcgaggg tttggatatg
    16561 ctctcgtcca tgcgcttgtg tgccaatacg ccgatgcagc acgcgattca gacggcattg
    16621 ggcggttatc agagcattaa cgaattcatc ttgcccggcg gacggctttt ggaacagcgc
    16681 aacagagcgt gggaactggt caaccagatt cccggcgtgt cctgcgtcaa accgatgggc
    16741 gcgatgtata tgttcccgaa aatcgatacc gaaatgtacc gcatccgcga tgatatgaaa
    16801 ttcgtttacg atttgctggt gcgcgaaaaa gtcctgctgg tacagggaac ggggtttaat
    16861 tggatcaaac ccgaccattt ccgcattgtt acgctgcctt acgtccatca gattgaagag
    16921 gcgatgggca ggttggcaag attcctgcaa acctaccatc agtaggattc tgataaaaaa
    16981 tgccgtctgg aacggagatt cccgtttcag acggcatttt caacagcagg aacaaatcag
    17041 gcaaatttca gtctgtcgcc gtcggcttct accttgattt cgctttcggg cgcatagttt
    17101 ccggcaagca gggctttggc cagcgggttt tcgatttccg attggatcgc gcgtttgagc
    17161 ggacgtgcgc cgtaaatcgg gtcgaaaccg gctttggcga tgatgtccag agcggcatcg
    17221 gaaacagtca ggcgcaggtt ttgtttttcc aaacgttttt ccaaaccttt gagctggatt
    17281 ttcgcaatac tgcggatatt ggcctgatcc aagccatgga acacgaccac ttcgtcgatg
    17341 cggttgatca tttccggacg gaaatgttct ttcacatcct ccatcacaac ttctttcacc
    17401 gcttcgtagt cctgcgtgcc catttgttgg atgtgctggc tgccgatgtt ggaagtcatc
    17461 acaatcacgg tatttttaaa atctaccgtc ctgccttgtc cgtcggtcaa acgaccgtca
    17521 tccaatactt gcagcaggat gttgaatacg tcgggatggg ctttttccac ttcgtccaaa
    17581 agaatcacgc tgtacggttt gcggcggact tgttcggtca ggtagccgcc ctcttcgtat
    17641 ccgacataac ccggaggcgc gccgattaag cgggcaacgg cgtgtttttc catgtattcg
    17701 gacatgtcga tgcggatgag gtggtcttcg ctgtcgaaca ggaagcctgc caaagctttg
    17761 cacaactcgg tcttacccac accggtcggg cctaagaaca ggaagctgcc gtaaggtttg
    17821 ttcggatcgg caaggccgga gcggctgcgg cggatggcat cggacacggc gcgcacggct
    17881 tcgttttgac cgactacgcg gcggtgcaac acttcttcca ttttcaacaa tttatcgcgt
    17941 tcgccttcca tcattttgga tacgggaatg ccggtcatac gggaaacgat ttcggccact
    18001 tcatccgcgc cgacattatt acgcaagagt ttgtttgccg gttttgtgct gtccgtatct
    18061 gcccgttcgg cggctgcacg ctgtttttcc aaatgctcca aatcttcata catcaatttt
    18121 gaagccagtg ccaaatcgcc ttgccgtttt gcctgttcga ttttaatttt gacttcgtca
    18181 atttgtttct taatattggc agcaccgtct gaaatcgctt tttcggcttt ccagatttcg
    18241 tccaaatcgg cgtattcttt ttgcaagccg tcgatttcct cgtctatcag ttccaaacgt
    18301 tttttgctgg catcgtcttt ttctttttca acgtgcgcct tttccatccg aagctgaatc
    18361 aaacggcggt cgattttgtc catagcttcc ggtttggttt ctttttccat cttgacacgg
    18421 ctggcagctt cgtcaatcaa atcaatcgcc ttgtcgggca aaaagcggtc ggtaatgtag
    18481 cggtcgctca actccgctgc ggcaacaatg gcggggtcgg taatatcgat gccgtggtgg
    18541 atttcatagc gttcctgcaa accgcgcagg atagcgatgg tgtcttccac gctgggttcg
    18601 ccgaccaata ctttttggaa acggcgttcg agtgccgcgt ctttttcgat gtattggcgg
    18661 tattcgtcca aagtagtcgc accgatacag tgcagttcgc cccgcgccaa ggccggtttc
    18721 aacatattgc ccgcgtccat cgcgccgtcg gttttgcccg cgccgaccaa agtatggatt
    18781 tcatcgatga aaatcagggt gttgccgtcg tctttcgcca aatcgttcaa tacgcctttc
    18841 aagcgttctt caaattcgcc acggtatttc gcaccggcaa tcaaagccgc caaatccaaa
    18901 accagcaggc gtttgttacg cagggattca ggtacttcgc cgttgacgat gcgttgcgcc
    18961 aaaccttcga cgatggcggt tttacccaca ccgggttcgc caatcagcac ggggttgttt
    19021 ttggtgcggc gttgcagcac ctgaatcgcg cggcggattt catcatcacg accgataacg
    19081 gggtcgagct taccgtctcg ggcgcgttgg gtcagatcga gcgtgtattt tttcaaagca
    19141 tcgcgttggt cttcggcatt cgcatcgttc acgttttgtc cgcctcgtac cgcatcgatc
    19201 gcggcattga tgttttgttc ggtcgcaccg gcttctttca agattttgcc ggccgcatcg
    19261 ttctgctgca ccaaggcgag caggaaaagt tcgctggcga tataggcatc gccgcgtttg
    19321 gttgccgctt tgtccatcag gttcaacacc gcctgcaatt cgcggctggg cagaatctca
    19381 ccaccttgtc cggacacttt cggcaggcta ttcaaatgct gctgcaaacg ctgtttaacc
    19441 tgcggcacgt tcacgcccgc atgagccaag agcgcggcag ctccgctgtt ttggtcgtca
    19501 agcagggctt tcagtacgaa acctgcttcc agatagctgc tgtccgcagc caacgccaaa
    19561 ctctgagctt ctgcaagggc ttgttggaat ttggcggtta atttgtcgta tcgcattttc
    19621 gtttcctttt caaaatgtcc gctgtcgaag cctatatgtg catgactgtg gataactcaa
    19681 gtgttgtttt ctgtttttct atatttaatt cgatatattg ttgaatttaa agtatataga
    19741 aatgtatgat aatatgtata actatatctt cttaatatgg aaaagtctgt tgtcggctgg
    19801 atgtatgtgg caaatcgggt ataatcggcg catcttttcc ctttcagacg gcattgctgc
    19861 cgtaaggaca tttttatgag caaaaaacga gttctgaccg gcgtaaccac caccggcacc
    19921 ccgcatctgg gcaactacgt cggcgccatc cgccccgccg tccgcgcggc gcaaaacccc
    19981 gataccgaat ccttcctctt cctcgccgat taccacggca tcatcaaatg ccacgagcag
    20041 gagatgattc accaatccac ccaagccgtt gccgccactt ggcttgcctg cggactcgac
    20101 cccgagcgca ccaccttcta ccgccaaagc gacattcccg aagtgatgga gttgaactgg
    20161 attctgacct gcatcaccgc caagggtttg atgaaccgcg cccacgccta caaagccgcc
    20221 gttcaagcca atgcggaaaa cgggcaggaa gaccccgatt tcggtgtgga aatgggtttg
    20281 ttcagttatc cgattctgat gactgccgac attttgatgt tcaacgccaa cgaagtcccc
    20341 gtcgggcgcg accaaatcca acacgtcgaa atggcgcgcg acattgccgg ccgcttcaac
    20401 caccgtttcc aagaactctt caccctgccc gaagtgaaaa tcgacgaaaa cgtcgaactc
    20461 ttggtcggtt tggacggacg caaaatgtcc aaatcctacg gcaataccat tccgctttgg
    20521 gaaaacgaca aaaaaaccca aaaatcggtc aacaaaatca ttaccaacat gaaagagccg
    20581 ggcgagccga aacagcccga cgaaagccct ttgtttgaaa tctataaagc cttctccacg
    20641 ccgtctgaaa cggcggaatt tacgcaaatg cttgccgacg gcttggcttg gggcgaggcg
    20701 aaaaaacttt cggcggcgaa aatcaacgcc gaactggcag aactgcgcga acgctacaac
    20761 gcattgacct ctaatccttc gcaaatcgaa gagattttgc aggcaggcgc gcaaaaagcg
    20821 cgcaaagaag cgcgcgaatt gttggataaa gtacgcgatg cggtcggcat ccgcccgttg
    20881 aaataaaccc aatgccgtcc gaacctccgc ctgccacgtt tcagacggca tttcagacgg
    20941 cattttgaaa ccatcaggag tgcggaatgc tgaaaatacc ttttgccgtg ttgggcggct
    21001 gcctgctgct tgccgcctgc ggcaaatccg aaaatacggc ggaacagccg caaaatgcgg
    21061 cacaaagcgc gccgaaaccg gttttcaaag tcaaatacat cgacaatacg gcgattgccg
    21121 gtttggcttt gggacaaagt agcgaaggca aaaccaacga cggcaaaaaa caaatcagtt
    21181 atccgattaa aggcttgccg gaacaaaacg ccgtccggct gaccggaaag catcccaacg
    21241 acttggaagc cgtcgtcggc aaatgtatgg aaaccgacgg aaaggacgcg ccttcgggct
    21301 gggcggaaaa cggcgtgtgc cataccttgt ttgccaaact ggtgggcaat atcgccgaag
    21361 acggcggcaa actgacggat tacctgattt cgcattccgc cctgcaaccc tatcaggcag
    21421 gcaaaagcgg ctatgccgcc gtgcagaacg gacgctatgt gctggaaatc gacagcgagg
    21481 gggcgtttta tttccgccgc cgccattatt gagttattcg gacatcacgg aatatatttg
    21541 gatttttcaa accttgcgga aaaagtccgc gccgccggga aactcaaaag gaggggatgt
    21601 gtgttacaat tttccgaact gttttcataa aatagttttc ggacgtgttt caatatggca
    21661 ttgatgccgc cttgttgttc gagaaaaaac cctttatatt taatataatg ggttttaact
    21721 aaacgggaaa ccgttttctc tccggccgat gggcaaaatc agccgattga tggaacacgg
    21781 tccggttttt aagcaaaacc ttaatcaaca actcaagaag gataagacat gaaaaaatta
    21841 ttgattgccg caatgatggc ggctgccttg gcagcttgtt cgcaagaagc caaacaggag
    21901 gttaaagaag cggcccaagc cgttgagtcc gatgttaaag acactgcggc ttctgccgcc
    21961 gagtctgccg cttctgccgt cgaagaagcg aaaggccaag tcaaagatgc tgcggctgat
    22021 gcaaaggcaa gtgccgagga agctgtaact gaagccaaag acgcggcagc cgaaaccaaa
    22081 gaagcggtaa gcgaagcggc taaagacact ttgaacaaag ctgccgacgc ggctcaggaa
    22141 gcggcagaca aaatgaaaga cgccgccaaa taatttgttg ccttggcaaa aatgacgaga
    22201 tgccgcctgc cggcaaccca aatgaaaccg cctgaagatt ttcaggcggt ttttgagttg
    22261 tatgcccctt gttttttacg cctgcatgac cgtgcaggga ggatgtgccg cgcatatcgg
    22321 gatattgcgg acagggaaat gccgtctgaa catcatttag acggcatttg gtttgtcaag
    22381 gttcagacgg cttcaacttt gaacggcata ttgatttttt gccattggac gctttcatat
    22441 tccaatgcgt cggcaatcag ggggtggcgt tccagccatt tcctgtcaat acgcaggatg
    22501 aagccgcagc tttccgtatc cgtacgcaac tgcatatttt tcgggaaacc cagatcttgg
    22561 cgcgaacggc agaacagggc ggcaaggcgc agggacaaaa cggcatacca caacatttcg
    22621 ttgccgctga taatgccgcc catttttttc atatcgccgc gatgaccgat gaccagttgg
    22681 gcaagtatgg tctgttcctt gcgtgaaaaa cccggcatat cggcgttttc gaggatgtag
    22741 gcggaatgct tgtgatagcc ggtgtgggcg atgtccaaac cgatttcatg cagcgcggcg
    22801 gcgcgtccga gatactgttg ccacaaggca agttcttgaa ccgtaacgtt tttggcatga
    22861 cagaggctgt ccataaaggt ttgcgcggtc tcggcggtgc gtttcgcctg attgaggctg
    22921 acgtggtagc ggtgttggaa ctcggcaacc gtttgtccgc gcatatcttc gtttaagccg
    22981 cgcccgatca aatcgtaaaa cacgccgtcg cgcagggcgg cttcggttac ggtcatcctg
    23041 tcgagtttca tttcctcaaa cgccgccatc atcacggcaa gtccgccggc aaaaacttcg
    23101 atgcgttccg gtttcaggtt ttcaaatttg gcttttttga ccgaaccggc ttcgatgatg
    23161 cgttcggcga gggcgcgcat tcccttgtag gtaatgtccg cctcttgggg catttcggcg
    23221 gcaagcacgt cgcggatgga ttttgccgaa cccgatgtgc cgacggcgaa atcccaacct
    23281 tcgcgcctca tatttttgct gatgcgctgg atttcgttgc gggcggcgga aatggcggct
    23341 tggaagtctt tggcggtgat tttgttttgg aagaagcgca ggctgtaggt tacgcagccc
    23401 aagggcaggc tttcggtaat gtcgggattc agcgtcgaac cgatgacaaa ttctgtcgaa
    23461 ctgccgccga tgtcgataac cagcattttg cccccgcagg gggggagggt gtggatcacg
    23521 ccggtataaa tcagccgcgc ctcttcgcgc ccggcgatga tttcgatggg gaaacccagt
    23581 gccgcttcgg ccttgggaag gaaatctgcg atgtttttgg cgacgcggaa tgtattggtt
    23641 gccacggcgc gcacctgctc ggggcggaag ccgcgcaggc gttcgccgaa ttttgccagg
    23701 cagtccaaag cctgttcctg cgaggatgtg ctgaggtttt tctgttcgtc cagaccggcg
    23761 gcaaaacgga ccatctgttt gaaggaatcg atgactttta attgtccgtt gttgttttcg
    23821 caaatctgga ggcggaaact gttggaaccc aaatcgacgg aggcgaggac gtttgcggga
    23881 ttggtggtca tggcggatac cggtggggga aaaacgcaat gttactctga cggcgcaggc
    23941 gttgacaata aatgatgcgg ctgtttttga ttctgctcac ggatgttgcc gacggtatgt
    24001 ttccgacagc aaaaaatgcc gtctgaaaaa cgttcggacg gcattttttg cgcttatttg
    24061 aaatcctttt ccacgctcat gaaaatctgc gtatttttgc gtgtgtaaaa acttttcata
    24121 ttgctgtcga ttttcagata gcggaaattg agttgcggcg taaagccctt ccaagagatt
    24181 ttgtcgtgcc gcaacgacag gtttgcctga tattcgtggt ctttgcgcgg gaagcggtac
    24241 acaattgtcc cgggtgcatc aaaagtcctg cgggtatagc gcaggtttgc ccgcagcccc
    24301 aagccgccgt cgaacgtttt gaccgcgccg acacgcaaac ccttgcggac ggaagcctgt
    24361 tccgcctctt tcgtcatgtc gtgcggccag tccgcaccgc cgtaaagcag ccggtctttc
    24421 ggcgcggaat acatcagcgt cgcgcccgcc agcggcatat ggctgccgta tcgggcggcg
    24481 gtgcggtctt cctgataatg cttccacata ttgcccgcgt tcagcgtcag ccgccagcgt
    24541 tcgcccaagc gtcgggagaa atcggcattg aagccgccga cgaaattgta tcggctgccg
    24601 cctaagaggt tttgcccgac aaacggcacg atgccgaacg agcgcgttac cgaacgattt
    24661 ttatagccga gcgacaggtg caggcactgt tcgctgaaat ctttgttatc ccaataatgc
    24721 acgccgccgc cgctgatgcc gccgtagagg aaatgatgcc cgcccgcatt gatttcgcgc
    24781 gacacgccta agccctagcg caaaccgtgt gccttttgcg gcaggctgtc ggcggttttc
    24841 gtccagcttc tgcccgcaaa ttcaatcgtt ttttcggacg aagcgtt
//
LOCUS       DAWTQS010000024        24636 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.024, whole genome shotgun sequence.
ACCESSION   DAWTQS010000024 DAWTQS010000000
VERSION     DAWTQS010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 24636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24636
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.024"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            206..2126
                     /locus_tag="WNA25_000996"
                     /pseudo
     CDS             206..2126
                     /locus_tag="WNA25_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712496.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            2183..3160
                     /locus_tag="WNA25_000997"
     CDS             2183..3160
                     /locus_tag="WNA25_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFA9823765.1"
                     /translation="MDMKRRDFLKMTAALAAAGVSPSLLTAGKEQFTVYGAPAMPSVT
                     IAVTALQGKLAKQADVSLKIWRSPDQLRAGVASGQFKVMMSPSNVGVNLRNQGQKVGM
                     VNILTNGITQLVCKGSAIASPQDLVGKKILVPFKNDMPDIVLQALLKKLKIDAHKVSI
                     TYAATPPEAVGLFPSKGYHAVILPEPMATASLLKGKTIGINVVHGFDLVKAWGQAFGT
                     KPLIPMAGIIANEEYFHAHKAQFDIFHQDLKNALNWILANRQNAAKIGKNYLPAPEPA
                     LVMGLDGARLTVSKGSEVKNEILKFYEILMQFNPELLGGKLPDNGFFLA"
     gene            3497..3634
                     /locus_tag="WNA25_000998"
     CDS             3497..3634
                     /locus_tag="WNA25_000998"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823766.1"
                     /translation="MNEDKYRENMTWDEIENVVGTLVSEERMLKSNSFLSIKNSKNRL
                     A"
     gene            3713..4482
                     /locus_tag="WNA25_000999"
                     /pseudo
     CDS             3713..4482
                     /locus_tag="WNA25_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776012.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            4509..5201
                     /locus_tag="WNA25_001000"
     CDS             4509..5201
                     /locus_tag="WNA25_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFA9823767.1"
                     /translation="MICLENVRFEILRDPIVRDFSLNLQQGEVKALFGPSGCGKTTVL
                     RLIAGLETPKSGTIRNTFHKTGFLFQENRLPENLTAMQNIAIFMDNPDEGEIVALAAK
                     VGLTAGDLNKYPTELSGGMAKRVAFLRLLLCGCDLALLDEPFVGLDRDLRDILVAMLV
                     EKIERQGMACILVTHDRFEAARLSHEIMLLSAKGMNVQNVITLPTPLSERDSAFEEVV
                     VAREFQGIHYYE"
     gene            5393..6007
                     /locus_tag="WNA25_001001"
     CDS             5393..6007
                     /locus_tag="WNA25_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HFA9823768.1"
                     /translation="MFSTVITAAVLYIATAVDLLVILLIFFARANTRKEYRDIYIGQY
                     LGSVILILVSLFLAFVLNYVPEKWVLGLLGLIPIYLGIKVAIYDDCEGEKRAKKELDE
                     KGLSKLVGIVALVTVASCGADNIGLFVPYFVTLDLVDLLVTLLVFLILIFVLVYTAQR
                     LANISGVGEIVEKFSRWIMAVIYIGLGLFIIIENNTIRTIISII"
     gene            6346..7398
                     /locus_tag="WNA25_001002"
     CDS             6346..7398
                     /locus_tag="WNA25_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HFA9823769.1"
                     /translation="MRQYLPYSVRWCFFINPGAIILHRQIFLELMLPAAYGGFLTTAL
                     LDRTGFSGNLKPAATLMAVLLLVAAVLLPFLPQLAAFFVAAYWLVLLLFCAWLIWLDR
                     NTDNFALLMLLAAFTVFQTAYAVSGDLNLLRAQVHLNMAAVMFVSVRVSVLLGTETLK
                     ECRLKDPVFIPNVIYKNIAITLLLHAAAELWLPAQTAGFTALAVGFILLAKLRELHHH
                     ELLRKHYVRTYYLLQLFAAAGYLWTGAAKLQNLPASAPLHLITLGGMTGGVMMVWLTA
                     GLWHSGFTKLDYPKLCRIAVSILFASAVSRAVLMNVNPIFFITVPEILTAAVFMLYLL
                     TFVPIFRANAFTDDPE"
     gene            complement(7445..9634)
                     /locus_tag="WNA25_001003"
     CDS             complement(7445..9634)
                     /locus_tag="WNA25_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFA9823770.1"
                     /translation="MIYHRIAVNVPLSDGLLTYSHSEPLPPGTRVLVPFRNKTVVGMV
                     WETDIAPDMDAARILSVQTVFVEEKPLSQSWRDLLAFTSRYYHYPTGQAVFAALPQGL
                     KETRAVEMPQPPLFYALNEAGRAQTPPPARFNKKAALWDALLSGEMTMAALKQANAQA
                     AKLIEDWAEQGWIETTEAAKPVLRPYRGQASHSEFVLNTDQQKASDEIQTALGRFRSF
                     LLYGITGSGKTEVYFDAMAKVLAQGRQVLFLLPEINLTPQLLKRVENRFADVPTAVLH
                     SRMAAGRRTQDYLRAMLGQAKLVIGTRLAVFTPLPDVGLIVVDEEHDGSFKQDNELRY
                     HARDLAVWRAKQGGCPVVLGSATPSLESWHKAQSGAYRLLQLTERAHASAQLPQVDIL
                     NIGRLKLDNGFSPQALQLLKQNFEAGGMSLVYLNRRGFAPALFCGDCGHTFGCPNCSA
                     KMVLHQRARQLRCHHCDHREPIPFKCPDCGNQDLTAVGHGTQRVEETLRAFLPKAAVV
                     RVDRDSTAHKNDWADLYRRIANDEIDILVGTQMLAKGHDFARLNLVIVLNADGSLYSA
                     DFRAPERLFAELMQVSGRAGRADKPGKVLIQTQLPEHPVFAAVKAQDYAVFAENELNE
                     RQMFAMPPFGFQTAVRADAPRVADAMEFLNAAKETLAPLLPESVSRFGAAPMLMVRLA
                     ERERAQVFLESTSRQDLHRAVSLWVQVLQQNRDGKIRWSVDVDPQEA"
     gene            9774..10559
                     /locus_tag="WNA25_001004"
     CDS             9774..10559
                     /locus_tag="WNA25_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HFA9823771.1"
                     /translation="MKTKLIKILTPFTVLPLLACGQTPVSNANAESAVKAESAGKSVA
                     ASLKARLEKTYSAQDLKVLSVSETPVKGIYEVVVSGRQIIYTDAEGGYMFVGELINID
                     TRKNLTEERAADLNKIDFASLPLDKAIKEVRGNGKLKVAVFSDPDCPFCKRLEHEFEK
                     MTDVTVYSFMMPIAGLHPDAARKAQILWCQPDRAKAWTDWMRKGKFPVGGSICDNPVA
                     ETTSLGEQFGFNGTPTLVFPNGRTQSGYSPMPQLEEIIRKNQQ"
     gene            complement(10658..12592)
                     /locus_tag="WNA25_001005"
     CDS             complement(10658..12592)
                     /locus_tag="WNA25_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HFA9823772.1"
                     /translation="MSLIECKNINRCFGSGENRVHILKDISLSIEKGDFVAIIGQSGS
                     GKSTLMNILGCLDTAGSGSYRIDGIETAKMQPDELAALRRERFGFIFQRYNLLSSLTA
                     RDNVALPAVYMGMGGKERSARADKLLQDLGLASKEGNKPGELSGGQQQRVSIARALMN
                     GGEIIFADEPTGALDTASGKNVMEIIRRLHEAGHTVIMVTHDPGIAANANRVIEIRDG
                     EIISDTSKNPEIPASNVGRIQEKASWSFYYDQFVEAFRMSVQAVLAHKMRSLLTMLGI
                     IIGIASVVSVVALGNGSQKKILEDISSMGTNTISIFPGRGFGDRRSGKIKTLTIDDAK
                     IIAKQSYVASATPMTSSGGTLTYRNTDLTASLYGVGEQYFDVRGLKLETGRLFDENDV
                     KEDAQVVVIDQNVKDKLFADSDPLGKTILFRKRPLTVIGVMKKDENAFGNSDVLMLWS
                     PYTTVMHQITGESHTNSITVKIKDNANTRVAEKGLAELLKARHGTEDFFMNNSDSIRQ
                     MVESTTGTMKLLISSIALISLVVGGIGVMNIMLVSVTERTKEIGIRMAIGARRGNILQ
                     QFLIEAVLICIIGGLVGVGLSAAVSLVFNHFVTDFPMDISAASVIGAVACSTGIGIAF
                     GFMPANKAAKLNPIDALAQD"
     gene            complement(12658..13836)
                     /locus_tag="WNA25_001006"
     CDS             complement(12658..13836)
                     /locus_tag="WNA25_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacA family efflux pump subunit"
                     /protein_id="HFA9823773.1"
                     /translation="MAKMMKWSAVAAVAAAAVWGGWHYLKPEPQAAYITETVRRGGIS
                     RTVSATGEISPSNLVSVGAQASGQIKKLYVKLGQQVKKGDLIAEINSTTQTNTLNMEK
                     SKLETYQAKLVSAQIALGSAEKKYKRQTALWKDDATSKEDLESAQDALAAAKANVAEL
                     KALIRQSKISINTAESDLGYTRITATMDGTVVAIPVEEGQTVNAAQSTPTIVQLANLD
                     MMLNKMQIAEGDITKVKAGQDISFTILSEPDTPIKAKLDSVDPGLTTMSSGGYNSSTD
                     TASNAVYYYARSFVPNPDGKLATGMTTQNTVEIDGVKNVLLIPSLTVKNRGGKAFVRV
                     LGADGKAVEREIRTGMKDSMNTEVKSGLKEGDKVVISEITAAEQQESGERVMGGPPRR
                     "
     gene            14062..14451
                     /locus_tag="WNA25_001007"
     CDS             14062..14451
                     /locus_tag="WNA25_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HFA9823774.1"
                     /translation="MKNVQKGFTLLELMIAVAILGILTLIAYPSYKTYIRRARLSEVK
                     STLLVNAQNLERYYRQKGTFKTYDTNKLKQNKYFNITLSKVSPDHFTLQAAPDTATNE
                     GETCTVTLNDGGIIAASGTNQSCPGFD"
     gene            complement(14545..18030)
                     /gene="smc"
                     /locus_tag="WNA25_001008"
     CDS             complement(14545..18030)
                     /gene="smc"
                     /locus_tag="WNA25_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFA9823775.1"
                     /translation="MRLTHIKLSGFKSFTDPTTIHVPGQLVAVIGPNGCGKSNVIDAV
                     RWVLGEASAKQLRGESMQDVIFNGAATRRPAPRASVELVFDNSDHSLQGAWGQYAEVS
                     IKRQLTRQGESTYFINNQTVRRRDITDLFLGTGVGARGYAVIEQGMISRIIEARPEEL
                     RAYIEEAAGVSKYKERRKETEGRLKDTREHLQRLGDLQNELARQVEKLEKQAETAERY
                     KSLTAQLNQQQDLLDYAQWQQSLAAADKATAQHQSLQAQQDETAAQVQALNNEVHALQ
                     TAEQSQQQAVHELSNKRGVLREQIARLEEQIRHRQNLHQRIERDKQAAQAQLQRIRQE
                     QQQIRVQLEENELQVEEKQTELAEWAMQVAEHEERLPELEEVQATLNAAFQTQQDEAN
                     RIRRELALKQQQLAHAEQTVAKHEERKGRLKQENQALNLPDEAETAAAQEAAALLQSR
                     QEHYEEQIIAAEEALHAAREAFQTASNRFQSLKQQHITLQAQQQALSQILSQQQEAAD
                     FWQATDHAAAPQLWQHITAPAEWQHALSVILAERLHARSVPNSFVPPAPLPQGQAAWL
                     SDDLSGGIKKSLPVQALLNQIQAQPPFQTALHHWLDGVLCAPDLSYALAHQSDLGTHQ
                     IWLTPEGHQVDKVSVLLYAKPAQESLIAQKARLDGIAAELENLAPGLSAAEAAFKQAE
                     AAVRSSEVQHKNLMQQQQQHTRQYSQAQQRTAELLARTNQGQIRREHIERELAQLAEE
                     QTVLQHTSDGLSDDIATLQEAAAELEHQQQTTAHSRQEQQGRLKQAQLALLEANRQYG
                     LAEVAVHKLNQQKQNYRQQIARLEQQTLDWQERRQELALAYETEFQNDEQHIKLDELT
                     ETVHTLDEEYIAVQEKLAQIQEQGREQYARVQTLQTKLPQLQAATQTALLQQQEALIN
                     AKRYHQNLTERAADLDVLEALAKESAKVLNSSIGSLTRQIEALGAVNLAALQELEEAR
                     ERDGYYRSQSEDVQAAIALLEEAIAQIDDKTKARFKETFDAVNGKVQTFFPTLFGGGE
                     ATLKMIGDDLLTAGVSIMARPPGKKNSTIHLLSGGEKALTAMSLVFALFSLNPAPFCL
                     LDEVDAPLDDANTSRFCKLVKEMSVQTQFLYISHNRLTMEMAEQLVGVTMQEKGVSRV
                     VAVDIKQALEMAEAV"
     gene            18419..19087
                     /locus_tag="WNA25_001009"
     CDS             18419..19087
                     /locus_tag="WNA25_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823776.1"
                     /translation="MGLRQKLRGIIPNLATSIGTSAEANAPGALALGGNSEASKKFSI
                     AEGYLASSDGYGAIAIGSAAKIKQLEKGTINHIVGNDNKGLYVDADGNVTKITVRTES
                     EKDILSRYGQTYGAMALGFRSSSHNLFASSFGAFSTATAIESLAVGDSSQSTGYRSAT
                     FGSHSRALAEESLALGYETRANAYGSVALGAESVANEENTVSVGSDTLKRKIVNVADG
                     TEDL"
     gene            19059..19544
                     /locus_tag="WNA25_001010"
     CDS             19059..19544
                     /locus_tag="WNA25_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA C-terminal domain-containing protein"
                     /protein_id="HFA9823777.1"
                     /translation="MSPMARKIYDAVNVRQLNRLSKRTNRVGASAAALASLKPAQLGK
                     NDKFAFSLGFGSYKNAQAVAMGAVFKPAENVLLNVAGSFAGPDRAFGAGVSWKFGGKP
                     TPAVAAQNAAHPAKVLQLRQEVAALRARQAETDRKLHKQAEMENELQQLRRALSELKK
                     H"
     gene            19753..20475
                     /gene="lpxH"
                     /locus_tag="WNA25_001011"
     CDS             19753..20475
                     /gene="lpxH"
                     /locus_tag="WNA25_001011"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFA9823778.1"
                     /translation="MRPSYFISDLHLSEKHPELTELLLRFLRSAAAGQARAVYILGDL
                     FDFWVGDDEVSELNTSVAREIRKLSDKGVAVFFVRGNRDFLIGRDFCRQAGMTLLPDY
                     SVLDLFGSNTLICHGDTLCTDDKAYLRFRRIVHCRRLQKLFLMLPLKWRTRLAAKIRR
                     VSKMEKQVKPADIMDVNAAFTARQVRAFNAERLIHGHTHREHIHHENGFTRIVLGDWH
                     NDYASILRVDGDGAVFVPPEEC"
     gene            20744..22330
                     /locus_tag="WNA25_001012"
     CDS             20744..22330
                     /locus_tag="WNA25_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFA9823779.1"
                     /translation="MALFLSIFPIVLLIWLMVKKNSMPSYVALPITAVLIYAIKLFYF
                     GDAGMLLNATAASGLVKTLTPITVIFGAIMFNRMMETTGCIDVIRKWLATISPNPVAQ
                     LMIIGWAFAFMIEGASGFGTPAAIAAPILMSLGFNPLKVAIFTLVMNSVPVSFGAVGT
                     PTWFGFAPLNLSAEDILAIGRQTGVMHFFAGFVIPVIGLGFIVPWSEIRKNLGFVAIA
                     VFSCTIPYAALAMVNEEFPSLVAGAIGLMVSVFAANQGWGLSKDHAKDPNAEKVPFAQ
                     VAKALAPLGMLIGMLVVTRIKQLGIKGILTSKEEWFSFQLPFDLSKITVSDSLTITFG
                     NIFGQDVSASYQTLYVPAWIPFVLTVWICILLYKTKFKDAWTIYAVTFNQTKKPLLAL
                     MGALIMVQLMLVGGDNSMVKIIGKEFAAMAGEHWVYFSPYLGAIGAFFSGSNTVSNLT
                     FGPIQQQIALDTGLSVTLILALQSVGGAMGNMVCLNNIIAVCTVLDVKNSEGAIIKKT
                     VIPMAIYGVIAVVAAMIFFL"
     gene            complement(22496..22699)
                     /locus_tag="WNA25_001013"
     CDS             complement(22496..22699)
                     /locus_tag="WNA25_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HFA9823780.1"
                     /translation="MDNLNPQEISVLPENLPLYCSGPDNEQWNGHPRVFLPLGEGESG
                     SVACPYCGTRYRLDGKMPHHYYA"
     gene            22815..23312
                     /locus_tag="WNA25_001014"
     CDS             22815..23312
                     /locus_tag="WNA25_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-mimic protein DMP19"
                     /protein_id="HFA9823781.1"
                     /translation="MTALTLPEDIRQQEPSVLLYTLVSAYLEHTAQTGDESLSCLSDD
                     QHTLTAFCYLDSQVEEGGFVQLIASGYGEYIFRNPLADSLRRWKIKAVPKVLDKAKAL
                     YEQHGETIETLADGGADIESLRKQFPDFEEWDGAYYEAAEQDLPLLAEHILSNRAAFA
                     HIGQA"
     gene            23394..24584
                     /locus_tag="WNA25_001015"
     CDS             23394..24584
                     /locus_tag="WNA25_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HFA9823782.1"
                     /translation="MFFKHIEAAPADPILGLGEAFKAETRPEKVNLGIGVYKDASGAT
                     PIVKAVKEAEKRLLESETTKNYLTIDGVADYNEQTQILLFGKDHEIIASRRAKTAQSL
                     GGTGALRIAAEFAKRQLNAQTIWISNPTWPNHNAIAKAVGIQDKPYRYYDAAKHCLDW
                     DGMIEDLNQAQKGDIVLLHGCCHNPTGIDPTPEQWETLAKLSAEKGWLPLFDFAYQGF
                     GNGLEEDAYGLRVFLKHNTELLIASSYSKNFGMYNERVGAFTLVAEDEETAARAHSQV
                     KTIIRTLYSNPASHGANTIALVLKNDDLKAQWIAELDEMRGRIKAMRQKFVELLKAKG
                     STQDFDFIIEQNGMFSFSGLTPEQVDRLKNEFAIYAVRSGRINVAGITDDNIDYLCES
                     IVKV"
BASE COUNT         5597 a         6753 c         6428 g         5858 t
ORIGIN      
        1 aaacgcgaac cattcggttc gcgttttttt caattgagat aaaagacaat agcatgacag
       61 agattccaaa ttcatctacc gtgatattcc caagcccaag ttccaactgt tgcatcggtt
      121 atctggaaat ttttcatatc attttcttat ttacttaaat tttttaataa gataataaat
      181 aataattatt atcatgagct aagaaatgaa actaaatact ctcacatggg ctttgatgac
      241 cgttttttcc gttgcgccat cttgggcaga acaaccggca aatactgaag aaatacaacc
      301 cgtcaaaacc ttctccccgc ccaaaccgat tgcaccgacc gccgcacaag gctatttccc
      361 cgaaaaccaa ttcgaccgct ccgaccgcag cgattattac tttgttaccg aaaacataga
      421 ccaagccttc cgtccgctga aggcaaacag cagtttttac ggcaaaagct tttacaactc
      481 cgttaccgcg caagcactcg gggcgaaggt atacggcgta gccaacctca atcgcaccaa
      541 ggcaaacggc tacaaagatg gcggtgggcg cgacactgat tggaaataca gccgcttcaa
      601 tcaggctttg gtactcggtt tcgtgccgtc tgaaaatcaa gaataccgcc tcacttatct
      661 gcacgacgac atcaacaacg accgccagcc gcaggtcgtc aacgacgcat tggacaccga
      721 acgccacatc tccaaactca acgtgcgttg gggcaatgcc gatttgagca atacggtcag
      781 cgcggaagca ggcgtcatca aactcaaacg ccatgccgac aattactcct tgcgaccaaa
      841 caacacgccg cagcaagtgt tcgtagaact tgaccgcaaa gtgtatgatt tctcgctcaa
      901 acacgatgcg gatttcggca aattccacaa cacggcagcc gtcagctacc gcaacgacag
      961 ccaaaacggc gaacgcaaca cccacactgc catgtgcgat ttcctcaacg gctaccgttt
     1021 cgccgatgtg cacatcgacc gctggtgcat tgccgatacc ttatcttaca agtttgacga
     1081 ccgacacaaa ctgggcttgg gcttaagcta cgaactcaac gaagcggaca ttcgcaaaaa
     1141 tacggcacag cctgaaaacc caataaaacc cggtttcccc ttcgcctcat cacaacaaat
     1201 ctggaaaacc cattacggct acgacttcaa cggcaaagtg cgccgtcatg ctttgtcagg
     1261 cgaactcaaa tacgatttca cgccgtctga aacgcaaaaa tacagcgttt ccctcgcgca
     1321 tttggaacgc atcggcgaca acaccgaacg cttcaactcg cttgctgcca tcgtgcaaaa
     1381 ccgtatgagc ggcatgctga tgaaccaaaa tccagccgcc gccattgcag gcaatccccc
     1441 tgctgaaaac tgaaaaacac aaccgcatca ggctcaccgc cgacagccgc aacgactact
     1501 acaacggcta catgaactcg ctcgcaggcg cgggctggaa cgtgggcggc acgcttgtgg
     1561 cggacaaagt caaagacctg attatttttg accgcgcaca cggacaaagc ggcacagctt
     1621 ccaaagacgg cggcatcatc acacgcaacg tggacgcacg attgtttacc gcgcaggcct
     1681 acgcgcgtta caacttcaat ccgcattggg cggcaggcat caaagccgcc tacaactacg
     1741 gacacaacga aaccgacggc aggccgccct atcaaatccg tccgtttgaa gccgccgtcc
     1801 aagccgacta caaaaactac tttgcccacg gcagctacaa catcggcgcg gcaacacgct
     1861 ttgtcgccaa acaaacgcgc ggtgattttg atatggcaag cggtctgggc atagacaaac
     1921 gcgaagccgc caaaggcttt accgttgccg acgtgtacgc aggcgtaaac atcaaagaca
     1981 aatacggctt gcgcttgggc gtgaacaacg tattcaacaa aaaatacgtc gaatacatca
     2041 gcggcgacca cgtcctcgcc ctatccccca gcgtagtgta tgcaccgggc aggacatatt
     2101 ggttgagttt gcatgcggca ttttaaaacc atgccgtctg aaaaatacct gcattggctt
     2161 attaagaatt aggagataaa aaatggatat gaaaagacgc gatttcttaa aaatgaccgc
     2221 cgcgctggca gccgcaggcg tttcgccttc tctgcttact gccggtaaag agcaatttac
     2281 cgtgtacggc gcaccggcaa tgcccagcgt taccattgcc gtaacggcgt tgcaaggtaa
     2341 actggcgaaa caggcggacg tgtcgctgaa aatctggcgt tcgcccgacc aactgcgcgc
     2401 gggcgtggca agcgggcaat ttaaagtgat gatgagtccg agcaatgtcg gcgtaaacct
     2461 gcgcaaccaa gggcagaaag tcggcatggt gaatattttg accaacggca tcacgcagtt
     2521 ggtctgcaaa ggcagcgcga ttgcctcgcc gcaggatttg gtcggcaaaa aaatcctcgt
     2581 gccgtttaaa aacgatatgc ccgacatcgt gctgcaagcc ttgttgaaaa aactgaaaat
     2641 cgacgcacac aaagtcagca ttacctacgc tgccacaccg cccgaagcag tcggactatt
     2701 tccaagtaaa ggttaccatg ccgtcatcct gcccgaaccg atggcaaccg ccagcctact
     2761 gaaaggcaaa accataggta taaacgtcgt gcacggtttt gatttggtaa aagcatgggg
     2821 gcaggcgttt ggcaccaaac cgctgattcc gatggcaggt atcatcgcca acgaagaata
     2881 tttccacgca cacaaggcgc agttcgacat cttccatcaa gatttgaaaa acgcgctcaa
     2941 ctggatattg gccaaccgcc aaaacgcggc gaaaatcggc aaaaactacc tacccgcccc
     3001 cgaacccgcc ctagtcatgg gcttggacgg cgcacggctg acggtaagca aaggcagcga
     3061 agtgaaaaac gagattttga agttttacga aatcctgatg cagttcaacc cggaactttt
     3121 gggcggcaag ctgccggata acgggttctt cttggcttaa agggaaagag gccgtctgaa
     3181 aagggtaggg tggttgtgaa tacctgattt atcatttttc agacggccta ttaaggcatt
     3241 gtcagcggcg tgggtcggat tcttgaatcc gacatttcgg ctaaacgggg ctgtcggata
     3301 caagtatccg acctactctt ttcagacggc ctatcagata atagttcgaa cagtacacaa
     3361 agagccgtcg gattgcctga aatgttgggt catgacccaa cctacgctta ctggggaaac
     3421 gggatcgaag tgtgaggtat gtggcggggt tgtttaataa tgaaatttta taactatttg
     3481 atagggtggt aatgccatga atgaagataa atatagggaa aatatgacat gggatgaaat
     3541 tgaaaatgta gtaggaactt tagtttctga agaaagaatg cttaaatcca atagcttttt
     3601 aagtattaaa aatagtaaga ataggttggc ttaatcacaa ccataaccca acgaaaatac
     3661 taaaattcca agctaaggct atcctttcca aaccatcccc atcaaccaaa ccatgattaa
     3721 aaccgacaaa atccgcaaac cgcagcctgc gctgttttac atcatcgact acctttggag
     3781 cggctttgcc ggtctgagcg tggcgatggt ggtggtggcg ttgtgggcgt ggggcagcgc
     3841 cgtgttcggc gagtttatgc tgcctgcgcc ggtagaggtg tttcaaaaat ctttggattt
     3901 attgaaacat tttcaggaaa atgaaatcgg gatttcgctg tggcggtcgg tagtgggtat
     3961 ttcggttgct ttgatagcag gattggcggc ggggctggtg gcaggcagtt ttaagacggc
     4021 gatggcgttg cttaagcctg tgattacgat tttgttagca atgccgccga ttatttgggt
     4081 ggtgatggcg ttgttttggt tcgggttcgg caatccgagc gtgttgttta ccatcattgt
     4141 gttggttgcg ccgctgacgt ttgcgagtgc ggcggtcgga atggcgagcg tgaacaagca
     4201 gcatgaggag ttgtttgacg cttataaatt aggccgtctg aaaaaaatcc gctatctgta
     4261 tatcccgcat ctgacgggct atgtgatttc cagcgtcggc gtggcggtgg cgatgggggt
     4321 gaaggcggtg atcatggcgg aactcttagg cgcgagcaaa ggcgtgggcg cgcggattgc
     4381 ggacgcgagg gcaatgctgt agacttcgac ggtaatggct tatgtggtgt tggtcatggt
     4441 atttgtgtcg ctgtttgaat acctgattcc aagcctttgt aaattttgtt tatgccgtgg
     4501 aggagatgat gatctgtctt gaaaacgtgc gttttgaaat tctccgcgac cccatcgtgc
     4561 gcgatttcag tttgaacctg caacagggcg aagtcaaagc cttgttcggg ccgagcggct
     4621 gtggcaagac gacggtttta cggctgattg cgggcttgga aacgccgaaa tcgggcacga
     4681 tacgtaatac tttccacaaa acgggttttc tgtttcagga aaaccgcctg ccggaaaacc
     4741 tgaccgcgat gcagaatatc gcgattttta tggacaaccc agacgaaggc gaaatcgtcg
     4801 cgctggcggc gaaagtcggg ctgactgcgg gcgatttgaa caaatatccg accgaattgt
     4861 ccggcggcat ggcgaaacgg gtggcgtttt tgcgcctgct gctgtgcggc tgcgacctag
     4921 ccttgctgga cgaaccgttt gtcggtttgg accgcgattt gcgcgatatt ttggttgcta
     4981 tgctggtgga aaaaatcgag cggcagggca tggcgtgtat actggtaacg cacgaccgct
     5041 tcgaagccgc gcgcctgagc catgaaatta tgctgctttc cgctaagggc atgaacgtgc
     5101 aaaacgtgat aaccctgccc acgccgctgt ccgaacgcga ttcggctttt gaagaagtcg
     5161 tggtggcaag ggagtttcag gggattcatt attatgagtg atgaggggat ttgagtgtct
     5221 gacaaacctt acggttcgtt gccctctccc taaccctctc ccacggggag agggttagca
     5281 agcctcaggc ttgcctcttt agcgaaagat acgaatctgt tatccgaacg cgattcggtt
     5341 tttgaaaaag ccgtggtgcc aagggggcag gggattcatt atgaggtgct ttatgttttc
     5401 gactgtgatt actgctgctg ttttatatat tgctacagca gtagatttgt tggtaatact
     5461 attaatattt tttgctagag caaatactag aaaagaatat cgagatattt atatcggaca
     5521 atatttaggt tctgtaattt taatattagt tagtttattt ctagcttttg ttttgaatta
     5581 tgttccggaa aaatgggtgt tgggtttatt aggtttaata ccgatttact taggtattaa
     5641 agttgctatt tacgacgatt gtgagggcga aaaaagagct aaaaaagaat tggatgaaaa
     5701 agggttgtca aaattagtcg gtattgttgc tttggttaca gttgctagtt gtggtgcaga
     5761 taatattgga ctttttgttc cttactttgt gactttagat cttgtcgact tattagttac
     5821 tcttcttgta tttttaatat tgatttttgt tttagtatat acagcacaaa gattggctaa
     5881 tatttcaggt gttggtgaaa ttgtagagaa gtttagtcgt tggataatgg ctgttattta
     5941 tattggttta gggttattta ttattattga aaataataca attcgaacaa taatatcaat
     6001 aatatgaatg acaagggcat ttcagtatct gacaaacctt gcggttcgtt tccctctccc
     6061 taaccctctc ccacaggaga ggggatcagg ttgctgaaaa tcagagggcg aaggattggg
     6121 gatcaaatgg aaggtaaaac ctgaaaatgt tcgggtttga cagcccgatt ccctctcccc
     6181 gtgggagagg gctagggaga gggcaagcaa gccgcaggct tgcctctttg gcgaaaaata
     6241 caaccctgtc cgcccaataa atccataccc gaaagcatct tcatacagaa tcaagccaag
     6301 cgatgaataa attttttacc caccctatgc gcccgttttt cgtcggtgcg gcagtacttg
     6361 ccatactcgg tgcgttggtg ttttttcatc aaccccggcg ctatcatcct gcaccgccaa
     6421 attttcttgg aacttatgct gccggctgca tacggcggtt ttttgactac cgctttgttg
     6481 gaccggacgg gtttttcagg caacctgaaa cctgccgcta ctttgatggc ggtgttgttg
     6541 cttgttgcgg ctgttttatt gccgttttta ccgcaacttg ccgcattttt cgtcgccgcc
     6601 tattggctgg tgttgctgct gttctgcgcc tggctgattt ggctcgaccg caacaccgac
     6661 aacttcgctc tgttgatgtt acttgccgca tttaccgttt ttcagacggc ctatgccgtc
     6721 agcggcgatt tgaacttact gcgcgcgcaa gtgcatttga atatggcggc ggtcatgttc
     6781 gtatccgtcc gcgtcagcgt ccttttgggc acggaaaccc tgaaagaatg ccgtctgaaa
     6841 gaccccgtat tcatccccaa cgttatctat aaaaacatcg ccatcaccct gctgctgcac
     6901 gccgccgccg aactttggct gcccgcgcaa accgccggtt ttactgcgct tgccgtcggc
     6961 ttcatcctgc tcgccaagct gcgcgaactg caccatcacg aactcttacg caaacactac
     7021 gtccgcactt attacctgct ccagctcttt gccgccgcag gttatctgtg gacaggcgcg
     7081 gcgaaactgc aaaacctgcc cgcctccgcg cccctgcacc tgattaccct cggcggcatg
     7141 acgggtggcg tgatgatggt gtggctgact gccggactgt ggcacagcgg ctttaccaaa
     7201 ctcgactacc cgaaactctg ccgcatcgcc gtctccatcc ttttcgcctc cgccgtttcg
     7261 cgcgctgttt taatgaacgt gaatccgata ttcttcatca ccgttcccga gattctgacc
     7321 gccgccgtgt tcatgcttta cctgctgacg ttcgtaccga tttttcgagc gaacgcgttt
     7381 acagacgatc cggaataaaa atgccgtctg aaacggtttt cagacggcat cggattgcca
     7441 atgatcaagc ctcctgcgga tcgacatcca ccgaccatcg gattttgccg tcgcggttct
     7501 gctgcaacac ctgcacccac aaactcacgg cgcggtgcaa atcttgtcgg gatgttgatt
     7561 cgaggaaaac ttgcgcacgt tcgcgttcgg caaggcgcac catcagcatc ggggcggcac
     7621 cgaaccggga gacgctttcg ggcaagagcg gggcgagggt ttctttggcg gcgttgagaa
     7681 attccatcgc atcggcaaca cgcggcgcat cggcacggac ggccgtctga aaaccgaaag
     7741 gcggcatggc gaacatttgc cgttcgttca attcgttttc ggcaaacacg gcgtagtcct
     7801 gcgctttgac ggcggcgaag acagggtgtt cgggcagttg ggtctgtatc agcaccttgc
     7861 cgggtttgtc ggcgcgcccc gccctgccgg acacttgcat cagctcggca aacagccttt
     7921 ccggcgcacg gaaatccgcg ctgtacaggc tgccgtcggc gttcaacacg ataacgaggt
     7981 tgagccgcgc gaaatcatgg cctttggcaa gcatctgcgt gccgaccaga atgtcgattt
     8041 cgtcgttggc gatgcggcgg tacaaatctg cccagtcgtt tttgtgcgcc gtactgtccc
     8101 tatcgacgcg gacgacggct gccttgggca ggaaggcgcg cagggtttct tcgacgcgct
     8161 gcgtaccgtg tccgacggcg gtcaggtctt ggttgccgca gtcgggacat ttgaacggga
     8221 tgggttcgcg gtggtcgcag tggtggcagc gcagttggcg ggcgcgttgg tgcagcacca
     8281 ttttggcgga gcagttcggg cagccgaagg tatggccgca gtcgccgcaa aacagcgcag
     8341 gcgcgaagcc gcgccggtta aggtacacca gcgacatacc gcccgcttca aagttctgtt
     8401 tcaaaagctg caaggcttgc ggtgagaagc cgttatcgag tttcagacga cctatgttga
     8461 ggatgtccac ttgtggcagt tgcgcggaag catgggcgcg ttcggtcagt tgcagcaggc
     8521 ggtacgcgcc gctttgcgcc ttgtgccaac tctccaagct gggcgtggca ctgcccaaca
     8581 cgacgggaca gccgccctgc ttcgcccgcc acaccgccaa atcgcgggcg tggtagcgca
     8641 attcgttgtc ttgtttgaac gagccgtcgt gttcctcatc gaccacaatc agcccgacat
     8701 caggcaaagg cgtaaacacc gccagccgcg tgccgatcac cagcttcgcc tgccccaaca
     8761 tcgcgcgcag ataatcctgc gtgcgcctgc ctgccgccat ccggctgtgc aacacggcgg
     8821 tcggcacgtc ggcaaaacgg ttttccaccc gctttaaaag ctgcggcgtg aggttgattt
     8881 cgggcaacag aaacaacacc tgtcgcccct gcgccaacac tttcgccatc gcatcgaaat
     8941 acacctcggt cttgccgctg ccggtaatgc cgtacagcag aaacgaccgg aagcgtccca
     9001 atgccgtctg aatttcatcg gaagccttct gctggtcggt atttaataca aattcagagt
     9061 gcgaagcctg cccgcggtac ggccttaaaa caggtttcgc cgcttccgtc gtttcaatcc
     9121 agccctgctc cgcccaatcc tcaatcaatt tcgccgcctg cgcgtttgcc tgtttcaacg
     9181 ccgccatcgt catttcgccc gacagcagcg cgtcccacaa agccgctttt ttgttgaacc
     9241 gagccggcgg cggcgtttgc gccctgcccg cttcgttcaa agcataaaac aacggcggct
     9301 gcggcatttc caccgcgcgc gtttccttca aaccctgcgg caatgcggca aatacagcct
     9361 gcccagtcgg atagtggtaa taacgcgacg taaacgccaa caaatcacgc cagctttgag
     9421 acagcggctt ttcttccaca aagaccgtct gaacgctcaa aatccgcgcc gcatccatat
     9481 cgggcgcaat atccgtttcc cacaccatcc cgacaacagt tttattgcga aaaggcacaa
     9541 gcacccgcgt tcccggagga agcggctcgg aatgcgaata agtcaaaagg ccgtctgaaa
     9601 gcggcacgtt tacagcgatg cgatggtaga tcatacaggc aggcaaatca tttttttgcg
     9661 tattttaaca gccggaagct attttcagac gataaattga gcctcggcgc aatgcaggcg
     9721 caacagaggt ttgatacaat gccacttttc caacacgctc aatcatcttt gatatgaaaa
     9781 ccaagttaat caaaatcttg acccccttta ccgtcctgcc gctgctggct tgcgggcaaa
     9841 cgcccgtttc caatgccaac gccgaatccg ccgtcaaagc cgaatccgcc ggcaaatccg
     9901 ttgccgcttc tttgaaagcg cgtttggaaa aaacctattc cgcccaagat ttgaaagtgt
     9961 tgagcgtcag cgaaacaccg gtcaaaggca tttacgaagt cgtcgtcagc ggcaggcaga
    10021 ttatctacac cgatgccgaa ggcggctata tgttcgtcgg cgaactcatc aacatcgaca
    10081 cgcgcaaaaa cctgaccgaa gaacgcgccg ccgatttgaa caaaatcgac ttcgcctccc
    10141 tgcctttgga caaagccatc aaagaagtac gcggcaacgg caagctgaaa gtcgccgtct
    10201 tctccgaccc cgattgtccg ttctgcaaac gcttggaaca tgagtttgaa aaaatgaccg
    10261 acgtgacggt ttacagcttt atgatgccca ttgccggcct gcacccagat gccgcgcgca
    10321 aggcgcaaat cttatggtgt cagcccgacc gtgccaaagc gtggacggat tggatgcgta
    10381 aaggcaaatt cccggtcggc ggcagcatct gcgacaatcc cgtcgcggaa accacttcct
    10441 tgggcgaaca gttcggcttc aacggcacgc cgaccctcgt cttccccaac gggcgcaccc
    10501 aaagcggtta cagcccgatg ccccaactgg aggaaatcat ccgcaaaaac cagcagtaaa
    10561 cccgcaatga aaaaggcttg gaaccatgtt ccaagccttt ttcaaatgcc gtctgaagcc
    10621 tgtcgcggca gcttcagacg gcatctttgt tcaaacctca atcctgcgcc aatgcatcta
    10681 tcggattgag tttggctgcc ttattggcgg gcataaagcc gaacgcgatg ccgattccgg
    10741 tcgaacaggc gaccgccccg ataacggatg ccgccgaaat gtccatcggg aaatcggtta
    10801 caaaatgatt gaacacgagg ctgacggcgg cggacaaacc tacgccgacc aagcctccga
    10861 tgatgcagat taacaccgcc tcaatcaaaa actgctgcaa aatattgccg cgccgcgcgc
    10921 cgattgccat ccgtatgccg atttctttgg tgcgctcggt aacggacacc agcataatgt
    10981 tcatcacacc gatgccgccg actaccaatg aaatcagggc gatggaggaa atcagcagct
    11041 tcatcgtacc ggtggtgctt tcgaccatct gcctgatgct gtcgctgttg ttcataaaga
    11101 agtcttccgt gccgtgccgt gctttgagca gctcggccag ccctttttcg gcaacccggg
    11161 tattggcatt gtctttgatt ttgacggtga tggagttggt gtggctctcg cctgtgattt
    11221 ggtgcatcac cgtcgtatag ggcgaccaaa gcatcagcac gtcggaattg ccgaaagcat
    11281 tttcgtcttt tttcatcacg ccgatgacgg tcaaggggcg tttcctgaac aaaatggttt
    11341 tacccaacgg atccgaatcc gcaaagagtt tgtctttgac attttggtcg ataacgacga
    11401 cttgcgcgtc ttctttcaca tcgttttcgt caaacagccg ccccgtttcc agcttcagcc
    11461 cgcgcacgtc gaaatattgt tcgcccacgc cgtacaaaga agcggtcagg tcggtattgc
    11521 ggtaggtcag cgtgccgccg ctcgaagtca tgggcgtggc ggaagcaacg tagctttgtt
    11581 tggcgatgat ttttgcgtcg tctatggtca gggttttgat tttgccgctg cgcctgtcgc
    11641 cgaagccgcg cccggggaag atgctgatgg tgttcgtccc catcgaactg atgtcttcga
    11701 ggattttttt ctgcgaaccg ttgcccaatg cgacgacgga gacaaccgaa gcgataccga
    11761 tgatgatgcc gagcatggtc agaagcgaac gcattttgtg cgccaatact gcttgcaccg
    11821 acattctgaa ggcttcgaca aactggtcgt aataaaacga ccacgaggct ttttcctgaa
    11881 tcctcccgac attgcttgcg gggatttcgg gatttttcga ggtgtcggaa atgatttcgc
    11941 cgtcccggat ttcgatgacg cggttggcgt tggcggcaat gccggggtcg tgcgtgacca
    12001 taatgacggt atgcccggct tcgtgcagcc tgcggatgat ttccatcaca tttttgccgc
    12061 tggcggtatc gagtgcgccg gtcggctcgt cggcgaagat gatttctccg ccgttcatca
    12121 gggcgcgggc gatggagacg cgctgctgct gtccgcccga gagttcgccg ggcttgttgc
    12181 cctctttact tgccaaaccc aaatcctgca agagtttgtc cgcccgcgcg gaacgctctt
    12241 tgccgcccat acccatatac acggctggca gcgcgacgtt gtcccttgcg gtcagcgagc
    12301 ttaagaggtt gtagcgttgg aagatgaaac cgaagcgttc gcggcgcaat gccgccagtt
    12361 cgtcaggctg cattttggca gtttcgatgc cgtcgattcg gtacgaaccg gaaccggcgg
    12421 tatccaaaca accgagtatg ttcatcagcg tggacttgcc cgaaccggac tgcccgatga
    12481 tggcgacaaa atcacccttc tctatcgaca gactgatgtc tttcaaaata tggacgcggt
    12541 tctcgccgct gccgaagcag cggttgatgt ttttacattc gatcaagctc ataatattcc
    12601 gtaaatcaat aacaaatgcc gtctgaaacc gtttccgtgt tcagacggca tattcgttta
    12661 tcggcgcggc gggccgccca tgacgcgttc gccgctttcc tgttgctcgg cggcggttat
    12721 ttcggagatg accactttgt ccccctcttt caaaccgctt ttcacttcgg tattcatact
    12781 gtctttcata ccggttcgga tttcgcgttc cactgccttg ccgtccgcac ccaacacgcg
    12841 tacgaacgcc ttgccgccgc gatttttcac ggtcagcgac ggaataagca acacattttt
    12901 cacaccgtcg atttcaaccg tattctgcgt cgtcatcccc gtggcgagtt tgccgtccgg
    12961 attcggcaca aacgaacggg cataataata gaccgcattg gaagccgtat ccgtactgct
    13021 gttgtagccg cccgacgaca tcgtggtcag cccggggtcg acgctgtcga gcttcgcctt
    13081 aatcggcgta tccggttcgg acaaaatcgt aaacgaaata tcctgccccg ccttcacctt
    13141 ggtaatatcg ccctcggcaa tctgcatttt gttcaacatc atatccagat tcgccaattg
    13201 gacaatcgtc ggcgtagact gcgccgcgtt cacagtctgc ccctcttcca cgggaatcgc
    13261 caccaccgtg ccgtccatcg tcgcggtaat gcgcgtgtag cccaaatccg actcggcggt
    13321 attgatggaa attttgctct gtctgattaa agccttcaac tcggcaacat tggctttggc
    13381 ggcggcaagc gcatcctgcg cgctttccaa atcttcttta gaggtcgcat catccttcca
    13441 caacgccgtc tgacgcttat atttcttctc cgcgctgccc aatgcaatct gtgccgacac
    13501 cagcttcgcc tgatacgttt ccaatttaga tttttccata ttgagcgtgt tggtctgcgt
    13561 ggtcgaattg atttccgcaa tcaaatctcc ctttttgacc tgttgcccga gtttgacata
    13621 aagcttttta atctgccccg aagcctgcgc gccgaccgat accagattcg acggcgaaat
    13681 ctcgcccgtc gcggaaaccg tccggctgat gccgccgcgc ctgaccgttt ccgtaatata
    13741 agcagcctgc ggttcgggct tcagataatg ccatccgccc caaaccgctg ccgccgcgac
    13801 tgccgcaaca gccgaccatt tcatcatttt cgccatattt tcaattctct gtatgcgtca
    13861 atcccataaa ccggaagcgg attctaccgc aagtccctgc cccatccaag gcggcaaacc
    13921 gccgacaata aaaataacca tttgttttaa atggcttata caatctgctt tcccgaaaat
    13981 ttcaatccgt ttacatattc cgccatttcc gatacggcgt tataatgccg cccacaaaca
    14041 accaaatgga aacacattgc tatgaaaaac gttcaaaaag gctttacgct gctcgagctg
    14101 atgattgccg tcgccatcct cggcatcctg acgctcatcg cctacccttc ctataaaacc
    14161 tacatccggc gcgcccgcct gtcggaggtc aaatccaccc tgctggtgaa cgcgcaaaac
    14221 ttggagcgtt actaccgcca aaaagggacg tttaaaacct acgacacaaa caaactgaaa
    14281 caaaacaaat atttcaacat taccttaagc aaagtcagcc ccgaccactt cacccttcag
    14341 gccgctcccg atacggcgac caacgaaggc gaaacctgca ccgtcacgct caacgacggc
    14401 ggtatcattg ccgcttccgg tacaaaccaa tcctgccccg gcttcgactg attggctgtt
    14461 gcagtcggca ttgtgcctaa accatctata aaaatgccgt ctgaacgctg ttcagacggc
    14521 atttttgaca tcccgatacg cctttcaaac ggcttccgcc atttccaacg cctgcttgat
    14581 gtctacggcg acgacgcgcg acacgccttt ttcctgcatg gttacgccga ccagctgctc
    14641 cgccatttcc atggtcaggc ggttgtggga gatgtagagg aactgggttt gcaccgacat
    14701 ttctttgacc agcttgcaga aacgcgaggt gttggcatcg tccagcgggg catcgacttc
    14761 gtccagaagg cagaaggggg cggggttgag gctgaacagg gcgaacacga ggctcatggc
    14821 ggtgagggct ttttcgccgc cggagaggag gtggatggtg ctgtttttct tgccgggcgg
    14881 gcgcgccatg atggacacgc cggcggtcag gaggtcgtcg cctatcattt tgagggtggc
    14941 ttcgccgccg ccgaacagag tcgggaagaa ggtttggact ttgccgttga cggcgtcgaa
    15001 agtttctttg aaacgcgctt tggttttgtc gtcgatttgg gcgatggctt cttccaaaag
    15061 cgcgatggcg gcctgcacgt cttcgctctg gctgcggtag tagccgtcgc gttcgcgcgc
    15121 ttcttcgagt tcttgcaggg cggcgaggtt gacggcgccg agggcttcga tttgccgggt
    15181 aaggctgccg atgctgctgt tcaatacttt cgccgattct ttcgccaacg cttcgagcac
    15241 gtccagatcg gcagcgcgtt cggtcaggtt ttgatggtag cgtttggcgt tgatcagggc
    15301 ctcctgctgc tgcaacaggg cggtttgggt ggcggcctga agctgcggca gcttggtttg
    15361 cagggtttgt acgcgggcgt attgctccct gccctgttcc tgaatctgcg cgagtttttc
    15421 ctgcacggcg atgtattctt cgtccagcgt gtgtacggtt tcggtcaact cgtcgagctt
    15481 gatgtgctgc tcgtcgtttt ggaactcggt ttcataggcg agggcaagct cttgccggcg
    15541 ttcctgccag tccagggttt gctgttcgag ccgggcgatt tgctgccggt agttttgttt
    15601 ttgctggttg agtttgtgga cggcgacttc ggcaagcccg tattggcggt tggcttccaa
    15661 cagggcaagc tgcgcctgtt tcagacggcc ttgctgctct tggcggctgt gcgcggtggt
    15721 ttgctgctgg tgttcgagtt cggcggcggc ttcctgcaaa gtagcgatgt cgtctgaaag
    15781 cccgtcggac gtgtgttgca acacggtctg ctcttccgcc aactgtgcca gttcgcgctc
    15841 gatgtgttcg cggcggattt gcccttggtt ggtgcgcgcc agaagttcgg cggtgcgttg
    15901 ctgcgcttga ctgtattggc gcgtgtgttg ctgttgctgc tgcatcagat ttttgtgctg
    15961 cacttcggac gagcgcacgg cagcctccgc ctgtttgaac gcggcttcgg cggcggaaag
    16021 tccgggggcg aggttttcca gttcggccgc gatgccgtcg aggcgtgctt tttgggcaat
    16081 caggctttcc tgcgcgggct tggcatagag caggacgctg actttatcga cctgatggcc
    16141 ttcgggtgtg agccagattt gatgcgtgcc caaatcgctt tgatgcgcga gggcgtagct
    16201 caaatcgggc gcgcacaata cgccgtcgag ccagtggtgc aatgccgtct gaaacggcgg
    16261 ctgcgcttgg atttggttca gcaatgcctg tacgggcagg gattttttga tgccgccgga
    16321 gaggtcgtct gaaagccatg ccgcctgccc ctgcggcaaa ggtgcgggcg gtacgaaact
    16381 attcggcacg ctacgggcgt gcaggcgttc ggcaaggatg acggacaggg cgtgctgcca
    16441 ctcggcgggc gcggtgatgt gttgccacag ttgcggcgcg gcggcgtggt cggttgcctg
    16501 ccaaaagtcg gcggcttcct gctgttgcga caggatttgc gacaacgcct gctgctgcgc
    16561 ctgcaaggta atgtgttgct gcttcaggct ttggaagcgg tttgaggccg tctgaaacgc
    16621 ctcgcgggcg gcgtgcaagg cttcttcggc ggcaatgatt tgttcttcgt aatgctcttg
    16681 ccggctttgc aacaaggcgg cggcttcctg agcggcggcg gtttcggcct cgtcgggcag
    16741 gttcagggct tggttttcct gcttcagacg gcctttgcgc tcttcgtgct tggcaacggt
    16801 ttgttcggca tgggcaagct gctgctgctt caacgccagt tcgcggcgga tgcggtttgc
    16861 ctcgtcctgc tgggtttgga aggcagcgtt gagcgtggct tggacttctt ccaattcggg
    16921 cagacgctct tcgtgttcgg caacctgcat cgcccattcc gccaattcgg tttgcttttc
    16981 ttcgacctgc aactcgtttt cttcaagctg cacgcggatt tgctgctgct cctgccggat
    17041 gcgttgcaac tgcgcctgcg ccgcctgctt gtcgcgttcg atgcgttggt gcaggttttg
    17101 ccggtggcgg atttgttctt ccaaacgggc aatctgctcg cgcaacacgc cgcgtttgtt
    17161 gctcaattcg tgtactgcct gctgctgcga ctgttcggca gtctgcaagg cgtgtacttc
    17221 gttatttaac gcctgaacct gtgcggcggt ttcatcctgc tgcgcctgca aagattgatg
    17281 ctgcgcggtc gccttgtcgg cggcggcaag cgattgctgc cattgggcgt aatcgagcaa
    17341 atcctgttgt tgattcaact gcgcggtcag ggatttgtag cgttcggcgg tttccgcctg
    17401 tttttccagc ttttccacct gacgcgccaa ctcgttctgc aaatcgccca aacgctgcaa
    17461 atgctcgcgc gtgtctttca gacggccttc cgtctccttg cggcgttcct tatatttgga
    17521 cacgcccgcc gcctcctcga tataggcgcg caactcctcc ggccgcgctt cgatgatgcg
    17581 cgaaatcatc ccctgctcaa tgacggcata accgcgcgcg cccacgcccg tacccaagaa
    17641 caaatcggta atatcgcggc ggcgcacggt ctgattgttg atgaaataag tcgattcccc
    17701 ctgacgcgtc agctgccgct tgatgctcac ctcggcatac tgcccccacg cgccctgcaa
    17761 actgtggtcg ctgttgtcaa ataccagctc caccgaagcc ctcggcgcag gacggcgcgt
    17821 cgccgcaccg ttaaaaatca cgtcctgcat actctcgccg cgaagctgct tcgccgaagc
    17881 ctcgcccaac acccagcgca ccgcgtcaat cacattcgac ttgccgcagc cgttcggccc
    17941 gataaccgcg acaagctgcc ccggaacatg aatcgtggtc gggtcggtaa aagatttgaa
    18001 gccggagagt ttgatgtggg tcaggcgcat aatggtcgga aaaaataaaa aagaagcgta
    18061 ttttaacgga aatcccgccg caccacccca tatcttgccg gcaaaacctt accgcatccc
    18121 gccccggtgc ggcaggattt tgcacatccc ggccatccgt attaaaatct ttgcgtttcc
    18181 cacactttcc tgacaaatat acggaggagg attccatgaa aacaaaaaca gtcaagcaag
    18241 caagcaagca agcaagcaag caagcaagca agcaaaatag tctaacccgc ctttctttaa
    18301 tcgctctact taatttccac ttctttttat cctgttcgat cttttgcggc tgcacctcaa
    18361 gaaataaact attcgttatc aggcggtaaa gtttacgcag ataagttcag tatcgccatt
    18421 ggggttgagg caaaagctca gggggataat accaaactta gcaacctcaa tcggcacatc
    18481 tgccgaagca aatgcacccg gggcattggc tttaggggga aattctgaag catcgaaaaa
    18541 atttagtata gcagaaggct atctggcatc tagtgatggc tatggtgcaa tagcgattgg
    18601 ttctgccgcc aagataaagc aattggaaaa aggcacaata aatcatattg tgggaaacga
    18661 taataaaggt ctttatgtcg atgcagacgg caatgtaaca aagataaccg taaggactga
    18721 gtcggaaaaa gatattctct caagatacgg tcaaacttac ggtgcgatgg cactagggtt
    18781 tagatcttct tcacataatc tttttgccag ttcatttgga gcgttttcta cagccacagc
    18841 tattgaaagc ttggcagtcg gcgacagcag ccaatcaacg ggctaccgca gtgctacttt
    18901 tggcagtcac agcagggctt tggcagaaga aagtttggca ttaggttatg aaactcgggc
    18961 aaatgcttat ggttccgttg ctttaggtgc agaatctgtg gcgaatgaag aaaataccgt
    19021 atcagtgggt tccgatacat tgaaacggaa aatcgtcaat gtcgccgatg gcacggaaga
    19081 tttatgatgc agtaaatgtc cgccagttga accgcttaag caaacgtaca aaccgcgtcg
    19141 gcgcaagtgc tgcggcgttg gcttcgttaa aacctgcaca attaggcaaa aacgacaaat
    19201 tcgccttttc tttgggcttt ggaagctata aaaatgccca agcggtggca atgggggcgg
    19261 tgtttaagcc cgctgaaaat gtgttgctta atgtggcggg cagttttgcc ggaccggacc
    19321 gggctttcgg cgcgggggtt tcttggaaat tcggcggcaa accgacacct gcggttgccg
    19381 cacaaaacgc ggcgcatcct gcaaaagttt tgcaattgcg gcaggaagtg gcggcgttgc
    19441 gcgcccggca ggcagaaacc gaccgcaaac tgcacaaaca ggccgaaatg gaaaacgagc
    19501 tgcaacagtt gcgccgcgcg ttgtccgaat tgaaaaaaca ttgaagcaat aaacgtaagg
    19561 cgggacggcg gctctgcctt tttccgatgc cgcacccgcg cgatgccgtc tgaagccttc
    19621 agacggcatt tttaacgcgc ccgaaccccg gttccgccaa tgcccgatgc ttggcgcggc
    19681 atttgccgta ttgccccgcc gccgggcgcg ttataatccg ccacgacatc cgcccgattt
    19741 ggaaacctgc ctatgcgacc ctcctatttc atttccgacc tgcatttgag cgaaaagcac
    19801 cccgaactga ccgaactgct gctgcgtttt ttacgttctg ccgccgccgg gcaggcgcgg
    19861 gcggtttata ttttgggcga tttgtttgat ttttgggtgg gcgatgacga agtttccgag
    19921 ttgaatactt cggttgcgcg ggaaatcagg aaattgtccg acaaaggcgt tgccgtgttc
    19981 tttgtcaggg gcaaccgcga tttcctaatc ggtcgggatt tttgccggca ggcgggtatg
    20041 acgctgctgc cggattactc ggttttggac ttgttcggca gcaacaccct gatttgccac
    20101 ggcgacacat tgtgcaccga cgacaaggca tacctgcgtt tccgccgcat cgtgcattgc
    20161 aggcggctgc aaaaactgtt cctgatgctg cccctgaagt ggcgcacgcg ccttgccgcc
    20221 aaaatcaggc gtgtcagcaa aatggaaaaa caggtcaagc ccgccgatat tatggatgtc
    20281 aatgccgcct ttaccgcgcg gcaggttcgc gcctttaacg cggaaaggct gatacacgga
    20341 cacacccacc gcgagcatat ccatcacgaa aacggcttta cccgcatcgt tttgggcgac
    20401 tggcataacg actatgcttc aatcctccgc gtggacgggg acggcgcggt attcgtgccg
    20461 ccggaagaat gctgaaaatg ccgtctgaag cctttcgggc ggctttttct acacgcccct
    20521 ttcccacact ccctccgccg catcatcacc atccttaacc cgcattaata gccattatcg
    20581 ataaaatgct tgaccgatta cataagatta cgtaaagtgt gcaaacgcat aataattggt
    20641 cttaccaatt tatcttttaa ccaaaatatc gggtgtgcgg gatttgaacg tctttccaaa
    20701 cctcgacgcg ccttatgatg caaatgccta aaaaggattc gttatggcac ttttcctcag
    20761 catattcccc atcgtcctgc tgatttggct gatggtgaaa aaaaacagta tgccttccta
    20821 cgtcgcactg ccgattaccg cagtgctgat ttacgccatc aaacttttct acttcggcga
    20881 tgcgggaatg ctgctcaacg ccaccgccgc ttccggcctc gtcaaaacgc tcacgccgat
    20941 taccgtgatt ttcggcgcga ttatgttcaa ccgtatgatg gaaaccacgg gctgcatcga
    21001 tgtcatccgc aaatggctgg cgaccatcag cccgaacccc gtagcgcaac tgatgattat
    21061 cggctgggct tttgccttta tgattgaagg cgcatccggt ttcggcacgc ctgccgcgat
    21121 tgccgcgccg attctgatga gcttgggctt caatccgttg aaagtggcga ttttcacttt
    21181 ggtgatgaat tccgtgccgg tatcgttcgg cgcggtcggt acgccgacct ggttcggttt
    21241 cgcaccgctg aacctgagtg ccgaagacat cctcgccatc ggcaggcaga ccggcgtgat
    21301 gcacttcttc gcaggtttcg tcatccctgt catcggtttg ggcttcatcg tgccttggtc
    21361 tgaaatccgc aaaaacttgg gcttcgtcgc cattgccgtc ttctcctgca ccattcctta
    21421 tgccgcattg gcgatggtca acgaagaatt cccgtcgctc gtcgccggcg caatcggcct
    21481 gatggtgtcc gtattcgccg ccaaccaagg ctggggcttg agcaaagacc acgccaaaga
    21541 cccgaatgcc gaaaaagtgc cgttcgccca agtcgccaaa gcactcgccc ctttgggtat
    21601 gctgatcggc atgctggtgg ttacccgcat caaacagctc ggaatcaaag gcattttgac
    21661 cagcaaagaa gaatggttca gcttccaact gccgtttgat ttgtccaaaa ttaccgtcag
    21721 cgactccctg acgattacct tcggcaatat tttcggacaa gatgtcagcg cgtcttacca
    21781 aacgctgtac gtcccggctt ggattccgtt cgtgctgacc gtttggattt gcatcctgct
    21841 gtataaaacc aaattcaaag atgcctggac gatttatgcc gtaaccttca atcaaaccaa
    21901 aaaaccgctg cttgccctga tgggtgcgct gattatggtt cagctgatgc tggtcggcgg
    21961 cgacaattcg atggtgaaaa tcatcggtaa ggaatttgcc gcaatggcgg gcgaacactg
    22021 ggtttatttt tcgccgtatc tgggcgcgat cggtgcgttc ttctccggct ccaacaccgt
    22081 gtccaacctg accttcggtc cgattcagca gcaaatcgcc cttgataccg gcctgtccgt
    22141 gaccctgatt ctggcgttgc agtccgtcgg cggcgcgatg ggcaatatgg tgtgcctcaa
    22201 caacatcatc gccgtatgta ccgtattgga cgtgaaaaat tccgaaggtg cgattatcaa
    22261 gaaaaccgtt atcccgatgg cgatttacgg cgtgattgcc gtcgtcgcgg caatgatttt
    22321 cttcctctag gcgaaacgcc gccatccata gcggcagcac gcggggaacg gaaaccgccg
    22381 ctcgtccggg cggcgcgtcg ggcagaatcc cggtgccgga acagcccgcc ctgccaaaca
    22441 aatgccgtct gaaaccggaa aaggcttcag acggcatttt tatcgccgtt tgcattcagg
    22501 cgtagtaatg atgcggcatc ttgccgtcaa ggcggtagcg cgtgccgcaa tacgggcagg
    22561 caacgctgcc cgattctcct tcgcccaaag gtaaaaacac cctcggatgc ccgttccact
    22621 gctcgttgtc cggtcccgag caatacagcg gcagattttc cggcaacacg gaaatttcct
    22681 gcggattcag attgtccatt tgattttcct ttgcgtggtt ggcgtgcgcc cattttacgc
    22741 catcggcagg ctaaaggata ttttcggcgc aaagccgcaa tccgctataa tccccacttt
    22801 tcagacggca taccatgact gcgcttaccc ttcctgaaga catccgccaa caagaaccat
    22861 ccgttctgct ctataccctc gtttccgcct acctcgaaca caccgcccaa accggcgacg
    22921 aatccctctc ctgcctgtcc gacgaccagc acacgctgac cgcattctgc tacctcgaca
    22981 gccaagtcga agaaggcggc ttcgtacagc ttatcgcctc cggctacggc gaatacattt
    23041 tccgcaaccc gcttgccgac agcctgcgcc gctggaaaat caaagccgtg ccgaaagtct
    23101 tggacaaagc caaagccctc tacgaacaac acggcgaaac catcgaaacg ctcgccgacg
    23161 gaggcgcaga catcgaatcc ctgcgcaaac agttccccga ctttgaagaa tgggacggcg
    23221 catactacga agccgccgaa caagacctgc ccctgcttgc agaacacatc ctgtcaaacc
    23281 gggcggcatt cgcccatatc gggcaggcgt gattgcgtct gtttccagcc cgtgtaaaac
    23341 agcgtaaaat cggcaaaccc gaattatctt ccgtccccat caaggagcaa gctatgttct
    23401 tcaagcacat cgaagccgcc cccgccgatc cgattctcgg tttgggcgaa gcgttcaaag
    23461 ccgaaacccg ccccgaaaaa gtcaacctcg gcatcggcgt gtacaaagac gcatccggcg
    23521 cgacacccat tgtcaaagcc gtcaaagagg ctgaaaaacg cctgttggaa agcgaaacca
    23581 ccaaaaatta cctgaccatc gacggcgttg ccgactacaa cgagcaaacc caaatcctgc
    23641 tgttcggcaa agaccacgaa atcatcgcca gccgccgcgc caaaacagcg caaagccttg
    23701 gcggtacagg cgcattgcgt attgcggccg aatttgccaa acgccagttg aacgcgcaaa
    23761 ccatctggat ttccaatccg acctggccaa accacaacgc cattgccaaa gcggtcggta
    23821 tccaagacaa accttatcgc tactatgatg ccgccaaaca ctgcttggat tgggacggca
    23881 tgattgaaga tttgaaccaa gcgcaaaaag gcgacatcgt cctgctgcac ggctgctgcc
    23941 acaaccctac cggtatcgac cctacgcccg aacaatggga aactttagca aaactttctg
    24001 ccgaaaaagg ctggctgcca ttgtttgact ttgcctacca aggcttcggc aacggtttgg
    24061 aagaagatgc ctatggcttg cgcgtgttct tgaaacacaa tacagaattg ctgattgcca
    24121 gctcttattc caaaaacttc ggcatgtaca acgagcgtgt cggtgcattc actttggtgg
    24181 ccgaagatga agaaacagca gcccgcgccc acagccaagt caaaaccatc atccgtacct
    24241 tgtattccaa cccggcttca catggtgcga acaccattgc actggtgttg aaaaatgatg
    24301 atttgaaagc acaatggatt gccgaactcg atgaaatgcg cggccgcatc aaagccatgc
    24361 gccaaaaatt tgtcgagttg ctcaaggcca aaggttcaac ccaagacttt gattttatta
    24421 ttgaacaaaa cggtatgttc tctttcagcg gcttgactcc cgaacaagtt gaccgtttga
    24481 aaaacgagtt tgccatttat gccgtccgtt ccggccgcat caacgtcgcc ggcattaccg
    24541 acgacaacat cgattatctg tgtgaaagca tcgtgaaagt atagtcaatt aaaatcaaaa
    24601 taggacagta gtgcatcgtc aaatcgggcg taatca
//
LOCUS       DAWTQS010000025        24505 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.025, whole genome shotgun sequence.
ACCESSION   DAWTQS010000025 DAWTQS010000000
VERSION     DAWTQS010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 24505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24505
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.025"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(109..267)
                     /locus_tag="WNA25_001016"
     CDS             complement(109..267)
                     /locus_tag="WNA25_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823783.1"
                     /translation="MMNPTEFLKARIAEWEAKSKEAGGNADFKAFEFAESEIKNYKAM
                     LKTYERPD"
     gene            complement(267..806)
                     /locus_tag="WNA25_001017"
     CDS             complement(267..806)
                     /locus_tag="WNA25_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02594 family protein"
                     /protein_id="HFA9823784.1"
                     /translation="MKELKWIEEARKYLGAHEKVNGKSNPVLLAMLQEMGNFNQEQKA
                     WWKETDTPWCGLFVGHCLGKAGRAVIRDWYRAKAWSMSGLTKLEAPAYGCIAVKPRRG
                     GGHVFFVVGKDAEGRILGLGGNQGNMVSIIPFDPADIDGYFWPSKLIGGKPVPSSPAE
                     GRYRLTAAAATGAQGAGEA"
     gene            1013..1249
                     /locus_tag="WNA25_001018"
     CDS             1013..1249
                     /locus_tag="WNA25_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HFA9823785.1"
                     /translation="MLRLQKWGNSAAVRLPARLLTALNAKIGDALETEIRNGELLIRP
                     VKRYRLSDLLAETEAAPPRAEGWEEMPDVGQEGV"
     gene            1249..1596
                     /locus_tag="WNA25_001019"
     CDS             1249..1596
                     /locus_tag="WNA25_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFA9823786.1"
                     /translation="MYIPERGDIFHLAFDPAAGTEMKGGHYAIALSPRAYNRATGLVY
                     ACPISQGRAAAARSGGMISTLLGAGTATQGNVHCHRMKALDWKIRRAAFRETVPDYVI
                     EDVLARIGAVLFD"
     gene            complement(1651..1803)
                     /locus_tag="WNA25_001020"
     CDS             complement(1651..1803)
                     /locus_tag="WNA25_001020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823787.1"
                     /translation="MKFSINREFRKMLKLFAEYAVLRRMFYAVLGAAPLTALIWKPAD
                     ILNALK"
     gene            complement(1964..2296)
                     /locus_tag="WNA25_001021"
     CDS             complement(1964..2296)
                     /locus_tag="WNA25_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823788.1"
                     /translation="MGFLQFGLLFAAAGGVLGAVWASLQERGRPVQAVLEAFISAIAA
                     AAAAERFVPLDQAWTCAAAGVFAGMMTGHALDTVRALAPKVLRGYLGGLAEKLTGVKD
                     GGSSDGKD"
     gene            complement(2297..2767)
                     /locus_tag="WNA25_001022"
     CDS             complement(2297..2767)
                     /locus_tag="WNA25_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823789.1"
                     /translation="MLRVLLNRRFLPAGFQAWLFGTATRVLEAVSGLGLSGYAAVFAL
                     APDEIYAWRIYYKFQDIPEAWTVGVLAAAGLLQTALLFARGVRACVASAYLLLFSGFV
                     WFLVSVAFWGAYPPLNTGMVVPPLLAFFCALAGNNALRFLFSAQKSRGLADEGS"
     gene            complement(2838..3143)
                     /locus_tag="WNA25_001023"
     CDS             complement(2838..3143)
                     /locus_tag="WNA25_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823790.1"
                     /translation="MIEDKKIVALNFAVEDGMTGASAAYHVVEHIGADYRNGYVTATL
                     NGYVSENAFKSGKRYLLARTLDFQETDITAPDPGWVYRKALEGAAGIPKDAQPVYAE"
     gene            complement(3255..7400)
                     /locus_tag="WNA25_001024"
     CDS             complement(3255..7400)
                     /locus_tag="WNA25_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFA9823791.1"
                     /translation="MGGKSSTVTSAEERILSLQVQRSSQGLTLPVIYGRTRVAGNLVW
                     YGDFVTIEHKTTTRRGGKGGGGVKQVGISYTYEAAVMLALCEGEIQGVGRVWRDKEKF
                     DSPAQLRLTLMRGGDEQPLWTHLQQAKHQGQALNYSGTAYLCSPNYELTKSAQIYQHN
                     FEVIGKSGYSGNIPDANPREIVLDLLTNQRYGCGFPSQNIGDTDRYSNYCRAVGIFLS
                     PAYTEQGEAQRNISELLEQTNSAAVFSQGRLKIIPYGDGSHSGNGAVYVADNKAAYDL
                     TDDDFIVSGAQDPVKAGRKTNADAFNQIQVEYLDRDNDYNVAIAEAKDQANIEQYGLR
                     PKDAVRMHGICDAKVAQKVAQQLLQRALYVRNEYEFKLGWKYCLLEPMDIVTLTDAGL
                     GLNKTPVRITEIEEDGEGVLSVKAEDCPAGVYTVSEYPTQPSSGYSADYNVSPGNAHV
                     PVIFEAPLQLTGGEPQIWLATAGGGMWGGAEVWVSADGDSYTRVGAVNRKARFGALTA
                     DLPDGAVFDRTNTLGVEISAGQLTGGTEQDSRDLLTLCYVDGEFLAYADAELKGVGRY
                     TLGNLTRGAYGSAVNAHAAGSRFARIDEALFKYAVPRNWIGRTVWVKLVSYNVFGGGI
                     QDLAEVPAYSYTIEGAPLGQIQNLRLTSSWAYGKEAVIAWDKLDGADTYDVEIYAGGS
                     RRRLRAVDGIVDNSYTYTQADMKADGGQVRGIVFKVRGRAVTGKTGNWAQIAAQNPQL
                     QALQGISIDSGLRQAFFTCQKPDEEDFAGIIVWVCENAACPAADANKAYDGAETFITI
                     AKCGGKPLEKGKTYYLRAAGYDSFGKDNLKISGSISFTVYDVSATDLSESSLNKALRD
                     KINLIDGNGAGSVNERVEAVRSTADGNAAAVQTHARSINGLEAQYTVKVDANGKVAGF
                     GLATTPKNGTPESKFIVNADRFGIGAAGKADVFPFVVDTQKNRVGINGELVVNGKAVV
                     DKLNAGDIHGGKIAADTLDANRLKAGSVTAREIGAAAVTADKIGANAVTADKIQVADL
                     SAVSSNLGSITGGSLNIGGGNFTVSSDGILTADNAVIRGRIEADSGYFNGTVRASSVE
                     GDVLRAHRLRWTEGNVWVLDLDKDPLPRVLIPNFYVVSETFGNNRVQAKLLLNGGVLA
                     PREVRETENYTNYIWRGRTFGRYEDLPPRARGGGDYTEQSGSRYRTRLEYQIQVIPAG
                     KPVSLKLSLASHESVFSPFVSVSYLAQSDYEYKQLLGRMVWRTFAESFRYDSKKQVYL
                     GGDRRVHDYQNQMYKHWNAYGGLLQLPDDIYGISFEYRPFTNADWSTMLAFDRSDKFV
                     VVKKYRGHAPQQYSLFQEEFNTAVPKSNLLFFVEHWWQYIELRNIRVLIPESRENEVW
                     QVG"
     gene            7640..7918
                     /locus_tag="WNA25_001025"
     CDS             7640..7918
                     /locus_tag="WNA25_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFA9823792.1"
                     /translation="MESQTFASVFDALCDTPAQAANMRLRAGLMMHIADTVRENGWTQ
                     KQAAEHCGLTRPRINDLLNGKIDKFSLDALVNINAGLGQCISLSFAPA"
     gene            complement(7946..8377)
                     /locus_tag="WNA25_001026"
     CDS             complement(7946..8377)
                     /locus_tag="WNA25_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFA9823793.1"
                     /translation="MPSETDLRARIVEEARSWLGTPYHHHAMVKGAGVDCAMLLVAVY
                     GAVGLLPEGFDPRPYPQDWHLHRDCERYLGFVTQFCRETESPQAGDIAVWRFGRSFSH
                     GGILAGGGKVIHSYIGRGVVSDDIGQAELIGRGVRFFTFSF"
     gene            complement(8379..9233)
                     /locus_tag="WNA25_001027"
     CDS             complement(8379..9233)
                     /locus_tag="WNA25_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2163 domain-containing protein"
                     /protein_id="HFA9823794.1"
                     /translation="MKAATKELIDLLHGGDEFQMADLYTITLSGGRVLRHTGADMPVV
                     WDGQAYGAHELVIKRGATRTAVGLEVDSNTLQISAAPDYRLEGLQWAEAALGGVLDGA
                     RVKIDRVFFDAGLRPVGAVNIFSGRVSDVSGGRSSVKVDVKSDIELLNVSSPRNIYQA
                     GCMRTLYDDGCKVNREKFTVNGRVTENSRTGTVLKHNLTQPDGWFSQGVIKFAGGRNA
                     GLSRTVKAHGGNTFELALRLPYPPQAGDAFKVYPGCDKRRDTCKDKFDNIVHFRGFPF
                     IPSADTVV"
     gene            complement(9230..9829)
                     /locus_tag="WNA25_001028"
     CDS             complement(9230..9829)
                     /locus_tag="WNA25_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2460 domain-containing protein"
                     /protein_id="HFA9823795.1"
                     /translation="MGNAVFPEFPGLKWGRKKTAVWSTGTQKSASGREFRTAYYTYPQ
                     WRFSLSFEVLRTKASVNELEKLAGFFNARKGSFESFLYEDPADNAVTDQPVGNTVQGV
                     ARYQLVRSMGGFIEPVSAVKERPAVKVGGTALAYGRDYTVTDKGVLVFNTPQPPGRPI
                     TWTGGFYFRVRFTSDTVDFENVLGSLWAAKKIEFTSVKL"
     gene            complement(9829..10095)
                     /locus_tag="WNA25_001029"
     CDS             complement(9829..10095)
                     /locus_tag="WNA25_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823796.1"
                     /translation="MIAHVCASTGWTWDYVADNLDLPRIKHLNEYWREHPPVHILAAS
                     YMGVKPSSGSVQSEADEAEAVGMLGGGELSEDEFDALLKAKGII"
     gene            complement(10107..10436)
                     /locus_tag="WNA25_001030"
     CDS             complement(10107..10436)
                     /locus_tag="WNA25_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823797.1"
                     /translation="MTVRIKGVTVELNGADYVIPPIALGALEQLQERIGSFDGNAADA
                     GQISTVIDCAHAALKRNYPDLTREEAADLIDIGNMNEVFAAVMDVSGLKRKEQEAAQA
                     GEARAAD"
     gene            complement(10497..11270)
                     /locus_tag="WNA25_001031"
     CDS             complement(10497..11270)
                     /locus_tag="WNA25_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823798.1"
                     /translation="MQLTFGSGEVFAQMITDAYGNRVQNATPVRIMGLQEMSVDLSAE
                     LKEFYGQNRYPLAVAQGKVKVSGKMKGALINGLTLNTLFFGTEYATGTMKALWAETTG
                     KVLDGDNYSYLQAAAPGGGKFAEDAGVMGQDGTAYIKVASSPKQGQYTVSESGVYAFN
                     SSDKGKTVYPSFTYTQTMPPAKKIELTNMATGNTPTFKMRYLTQFKGKKALLELESVT
                     SGKLGLFSTKNDDFSVPEIDFTASTDEAGFKIGTLWIQE"
     gene            complement(11296..11724)
                     /locus_tag="WNA25_001032"
     CDS             complement(11296..11724)
                     /locus_tag="WNA25_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823799.1"
                     /translation="MNREAVYSALWAKLDALDGFVTKSRKLVHWNDVKRYDQPALFMA
                     QGDMQALTLTGRETKWILRADVYLYVQTAGQPPAPVMNPLIDAVCNAVNAVHPVTGKT
                     DLTADGADIEYCRVEGTVETDEGTLGEQAVCIIPIVICAA"
     gene            complement(11721..12203)
                     /locus_tag="WNA25_001033"
     CDS             complement(11721..12203)
                     /locus_tag="WNA25_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFA9823800.1"
                     /translation="MLKMEFIGGGILAAVFKAYAADVQDAVVKSVGRSALRLQREVKQ
                     NRLSGRVLRVRTGNLRRSVHQRVNVSGNVVSGEVDTNVRYGIAHEYGFAGSVNVKASL
                     RQVRQAFGKPLKPPRYVRVRAHTRDVKLPERSFLRSALRDLTPKFADDLQKSIGKVLK
                     "
     gene            complement(12203..12733)
                     /locus_tag="WNA25_001034"
     CDS             complement(12203..12733)
                     /locus_tag="WNA25_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HFA9823801.1"
                     /translation="MAAPVSLEEFKQRIGVEHDRRDDFFLSVIDGVSAAAEAYIGRSL
                     LAADYVGRYDGNGKDRIVLDNYPVLSVSSVKINGADAGGWEFDNWLLMRPEGFARGLK
                     NVEVSYRAGYERMPADIREAVMIIAVQRVNEIEGKGVRSKTLAGETVAFSTFGNSGGM
                     PPSAFAILNEYKRKGV"
     gene            complement(12736..13068)
                     /locus_tag="WNA25_001035"
     CDS             complement(12736..13068)
                     /locus_tag="WNA25_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823802.1"
                     /translation="MPSENQTAFSFGDFEMTVKLKAPEGFTDVSFGSQSYAADENGIV
                     EVPSEAAEFLYQFGFGNVASEPAEGPEKAKRGRKPKTGQPAGQSEPAEAAEPAEAEAE
                     PAEAEKAE"
     gene            complement(13075..14574)
                     /locus_tag="WNA25_001036"
     CDS             complement(13075..14574)
                     /locus_tag="WNA25_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823803.1"
                     /translation="MNVNQLTQETIELMKSAQAGGGPPDKGFTQPAGFTAGLQTYDLS
                     APSQKLYPVLTPLRNRIPRVGGGRTIGSNWKAVTNINVGNQRAGIGEGRRGGVINHET
                     VERNAQFRAIGLENQVTFEADYAARGFEDVKALAVAQTLQATMVAEEMILLGGNTSLK
                     AGVTPTPTAVVSADAAGKISVSTLSVICVALGLQAYWDVAGANNGATGQSPNIKTAQV
                     PAKITRRNADGTTDTFGGGSARKSAAASVSGIEAGKKVTAVIPAVRGAVAYAWYWGAA
                     GSEKLGAVTTAAKVEISADAEGTQTAASLPSEDNSTSILEFDGLLTQIALPDSGAFWS
                     DNKGGGLTSDNAGGVYEFEEAFAHFFTRYRLSPDTVYVNARDLAALTKLIIGNSGAPL
                     IKLNVDVNNTANIRAGVVVGSYMNKITGDDLNIVVHPNLPAGTYLFYSSRLPAYVQGV
                     GNLLQVRTRQEYYQIEWPLRTRMYEYGVYADEVLQGMFMPAFGMITNVG"
     gene            complement(14615..15772)
                     /locus_tag="WNA25_001037"
     CDS             complement(14615..15772)
                     /locus_tag="WNA25_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFA9823804.1"
                     /translation="MTKLYAQIAKTEAQDDGTVKVWGYASSEAVDSDGEVVAAEAMKA
                     AIPDYMKFGAVREMHGSNAAGTAIEINVEDDGRTFFGAHIVDPVAVTKVKTGVYKGFS
                     IGGRVTARDDLNKSQITGLKLTEISLVDRPANPDAVFTCFKADKPKDEAGAADKDGKP
                     SDKPTEEEDENPKDGDKGPKTEDKGDKDAGKKDEAGKSASVELSESEIAALKAVLAKA
                     DKPKGGTAAKSMYQVKSPADVLMSLKWLVEDASYDNIDEAVTAQIKESAAGLAESLKA
                     LAAREADKPADGLAAKAGKSGDLAKAESADELAKAQDALKKSNDALAKAQAEIESLKK
                     QAVPPKGSTKAISKAEDNGEDPLKGFQPIVKNDGTLDDVATLIKAKQTGRL"
     gene            complement(15840..17987)
                     /locus_tag="WNA25_001038"
     CDS             complement(15840..17987)
                     /locus_tag="WNA25_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFA9823805.1"
                     /translation="MSKKTPLSQGFIARVAAGVRYAFTGNADGWFDAGEPLAPVAQQA
                     EGRRFDYEPFYNVGHSKPREREAVGFAQLRALADNYDVLRLVIETRKDQMECLKWTIQ
                     KRDVESTKDNESQRKDRKVDEAIAFFQSPDKEHTWADWLRILLEDLFVIDAPCIYPRK
                     TLGGDLYALEVIDGATIKRVLDNTGRLPLPPDTAYQQILHGMAAVDYTADELIYRSRN
                     NRSYKVYGYSPVEQIIMTVNIALKRQIHALEYYTAGSVPDALIGVPETWAMEDIKRFQ
                     EYFDLLLSGETAERRKLRYVPGELARNFKETKQPPLKDVYDEWLARVVCFAFSVEPTP
                     FVAQVNRSVAETSREQSLSDGMSSLKNWVKALIDDVLARYMNMAAYEFVWKEEESLNP
                     KEQAEIYAIYKNAGILTADEIRAELGKEPLPDQEEPEPNRQDDWKPEKQPNQEAEKLG
                     KSESPMSEDESAALIEAYLLTRIDGLAEQIAALIDSAAVDWQAEDLAAELSRAAGVVA
                     NGLDFGDWSGLSDVVEPIIRRVAEDGAVAALLRVMPEPAAGMVTNIRSRAVKWAHERA
                     AEMVGMKRAGGGLVRNPAAEWQITEGTREMIRAQVAEAMRNGDSVQELAGRLKESHAF
                     GNARARTIARTETAMADGMGNLIGWEGTGLVAGKQWITAKDDKVSDVCNANGGMGVIG
                     LHEPFSHGALTIPGHPNCRCAVVPVLAGDMPES"
     gene            complement(17984..19405)
                     /gene="terL"
                     /locus_tag="WNA25_001039"
     CDS             complement(17984..19405)
                     /gene="terL"
                     /locus_tag="WNA25_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HFA9823806.1"
                     /translation="MALGQFDDVETSVIRSLSSASLYMFTRRMFYQRRGYVWQRANHH
                     APICNALERVFNGETKRLIINIPPRYSKTEIAVVNFIAWAMGRVPDCEFIHASYSAAL
                     AVNNSVQIRNLVQHEEYRAIFPDLALAGESGHHWKTTAGGVMYATGAGGTITGFGAGK
                     HREGFGGCIIIDDPHKADEARSEVRRQNVIDWFQNTVESRKNSPDTPIILIMQRLHEK
                     DLAGWLLDGGNGEEWEHLCLPAIQDDGTALWPEKHDIETLHRMEQAAPYVFAGQYLQK
                     PAPPDGGTFKPDNLQFVKALPAGNIRWVRAWDLASTANDGDYTAGGRLGVTEDGRYII
                     ANIVRGQYGADERDRILRNTAQKDGVKTKISIPQDPGQAGKSQTLYLTRQLAGFSVSA
                     SPESGDKVTRAEPFAAQVNIGNVMVLDDGTWGTDALIAEMRMFPNGQHDDQIDCLSRA
                     FGELLDTRTGMIDFLQSQVEAMK"
     gene            complement(19467..19916)
                     /locus_tag="WNA25_001040"
     CDS             complement(19467..19916)
                     /locus_tag="WNA25_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823807.1"
                     /translation="MSGTKRKLGRPTDYTKDMADKICEKIANGRSLRSICAEDGVPPM
                     KTIYRWLEANEEFRHQYARAREKQADYFAEEIIEIADSAQAESAAVSKAKLQIDARKW
                     AASKIAPKKYGDKTELDVKSGDGSMRAAVRLDAEEYRKIAEDVLRRV"
     gene            complement(20209..21078)
                     /locus_tag="WNA25_001041"
     CDS             complement(20209..21078)
                     /locus_tag="WNA25_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823808.1"
                     /translation="MNVIQSFNFNTTSPVRAFEKDGLTWFVAADVCKALEIQNPTQAL
                     EKLDDDERSMFNIGRSEIHGGGGNVNIINESGLYILILRSRKAMEQGSTAWKFRKWVT
                     SEVLPTIRKTGGYQVGQKTTADDRTGLRQAVAALVGRKGIDYSSAYSMVHQRFNVEAI
                     EDIPAGKLPEAVAYVHALTLHTGLAGEVPDREPLPAPQPALPISGNALYDLAVAVSYG
                     ARAIQMGRDVSLPLKQLGCRQAVTMWTVWAETRSRLKAAANALEALSAHADAEHAEKI
                     RPILPEIRNLSAV"
     gene            complement(21360..22520)
                     /locus_tag="WNA25_001042"
     CDS             complement(21360..22520)
                     /locus_tag="WNA25_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HFA9823809.1"
                     /translation="MNAAAVSAVFKERIASHAEHVKKVAHICTTEETTKQALILPLLD
                     ILGFSAFDPNKVRAEYQADFPGAKSGERVDYALFCNGAPVMFIEAKSYTENLSNHCPQ
                     LSRYFNATPEIAICAITNGREWRFFTDLSNKNIMDSEPFLTVDVTMLNENDMAQLYQF
                     RHDKFQPDALRSLAEESIYLTAFTESITESLKEVDLDFVRYVAGRANIQRQFTQRYLE
                     SIRHIVKQAVQNTVSSMVVSGLSAPKVQEEAAPVEKEQEDPTAPIIDPENNKIVTTYS
                     ERRLFDLVKSILPDDASIEAKDTESYFGVLVDGKSNRWILRYFDNKQRPSVIFPIELE
                     ESDISNIERCGLEVSGNQVIIDTPENLLRVAWLVIDSYRFCCDDENFKRKPK"
     gene            complement(22611..23018)
                     /locus_tag="WNA25_001043"
     CDS             complement(22611..23018)
                     /locus_tag="WNA25_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823810.1"
                     /translation="MNQQEFEFMNDLARAFERRYRDTRSLNRCLSIEGRYMGEEACPH
                     KPEIGLRYGEDAMFLTLQAWAKVDAPQQEAVRISFGIGAKSQAAYEERLQAEIRRRGE
                     GPLHLQTDLGLAAWYRAIRQAAGNDFDLLFEKV"
     gene            complement(23285..23830)
                     /locus_tag="WNA25_001044"
     CDS             complement(23285..23830)
                     /locus_tag="WNA25_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFA9823811.1"
                     /translation="MQNLPQQGSPAKARGKTRGGGSRRDDGGKAARIHPRRARRLPDI
                     GRRPVDATGRGMRVIRLILPYPVSANRYWRIWRNRAVRSAEAAAYKSVVRRIAQEAGA
                     MPSEGAVAVYVRLIPKANKDGGANKTVIDLDNALKVALDALQGVAYHNDRQVRRIAAD
                     YADEPVAGGGLAVEVGELDEK"
     gene            complement(23966..24115)
                     /locus_tag="WNA25_001045"
     CDS             complement(23966..24115)
                     /locus_tag="WNA25_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823812.1"
                     /translation="MDTLLSIITALSFAGAATLAVWLLVEASDAVLRRKRDGKGEDDF
                     DGFGY"
     gene            complement(24292..>24505)
                     /locus_tag="WNA25_001046"
     CDS             complement(24292..>24505)
                     /locus_tag="WNA25_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823813.1"
                     /translation="AGLKDCGFDGGTEAALLAVISAAYYIRDGEDNFAWAAACVEDLL
                     EKMPPEAGRAPHPESPMPPETAGGGI"
BASE COUNT         5189 a         7560 c         6128 g         5628 t
ORIGIN      
        1 tccagcatag ccgcaccgta tccgcgccgg tattgcgcgg cgcggtcgta ttgccacgcg
       61 ccggcaacga acacggcgca aaccgacacc gcaatcagct tcgggttttt aatcaggccg
      121 ttcataagtt ttcaacatcg ctttgtagtt tttgatttcg ctttcggcaa actcaaaagc
      181 tttaaaatcg gcgtttccgc ccgcctcttt gcttttggct tcccattcgg cgatacgcgc
      241 tttcagaaac tcggtaggat tcatcattac gcctctcccg cgccttgcgc gccggtggcg
      301 gcggcggcag tcaaccggta acgcccttcg gcgggggacg acggcacagg tttgccgcca
      361 atcagcttgg acggccagaa gtagccgtca atgtccgcag ggtcaaacgg gatgatggat
      421 accatattgc cctgattgcc gcccaagccc aagattctgc cttccgcgtc tttgccgaca
      481 acgaagaaca cgtgtccgcc gccccggcgc ggtttgaccg cgatgcagcc gtatgcgggg
      541 gcttcgagtt tcgtcaaacc cgacattgac caggctttgg cgcgatacca gtccctgatg
      601 accgcgcgtc ccgctttgcc caggcagtgt ccgacaaaca ggccgcacca cggggtatct
      661 gtttctttcc accacgcttt ctgctcctga ttaaaatttc ccatttcttg cagcatcgct
      721 aaaagcacag ggtttgactt gccattcact ttttcgtgag cgccaaggta tttacgggct
      781 tcttcaatcc atttcaattc tttcatttct tttccttccg ataaaagacg agatgtaaac
      841 tttcggttta caccccggcc aagcctttga ttgcaggttt ccgcaacacc gatacatccg
      901 gtttcacacg ttccgtttcc atctccactc ctgttacacg ccgccgcttg gcaaaatccg
      961 tttcaggcgg cataatgtat cgacaacgta tctacaacac gaaagcaaaa ccatgttgcg
     1021 actgcaaaaa tggggcaaca gcgcggcagt ccgtctgccc gcccgcctcc tgaccgccct
     1081 caacgccaaa atcggcgacg cgctggaaac agaaatccgc aacggcgagc tgctcatccg
     1141 ccccgtcaaa cgctaccgcc tgtccgacct tttggcggaa acggaagccg ccccgccgcg
     1201 cgcggaaggc tgggaagaaa tgcccgatgt cggacaggag ggcgtttgat gtatattccc
     1261 gagcgaggcg acattttcca tcttgccttc gaccccgccg ccggtacgga aatgaaaggc
     1321 ggccattacg ccatagccct gtcgcccaga gcttacaacc gcgcaaccgg cttggtttac
     1381 gcctgcccga tttcacaagg cagggcggcg gcggcaagaa gcggcggtat gatttccaca
     1441 ctgctcggcg cgggaacggc aacacaaggc aatgtccact gccaccggat gaaggcattg
     1501 gattggaaaa tccgccgcgc cgccttccgc gaaaccgtcc ccgattacgt gattgaagac
     1561 gtattggcac gcatcggcgc agtactgttc gactgacttc agagtgcgaa gcctgtccgc
     1621 agggggaagc atccgtttcc atacccgccc ctatttcaaa gcattcaaaa tatccgccgg
     1681 cttccaaatc aacgccgtca aaggcgcggc gcccaaaacc gcataaaaca tacggcgcaa
     1741 caccgcatac tcggcaaaca gcttcaacat cttgcgaaac tccctgttta tgctaaactt
     1801 caaacagatt tctccttacc tcggataagg ttaaattcag aaacgccgca gggattccgc
     1861 ccctcgcggc gtttcgccgt tttcaaacct gccgcgtaat gtcgtgcgtc agccggacgc
     1921 gcccggaaca cagcgtcttc acgcgccgcg ataccgctcg aaatcaatct tttccatccg
     1981 aagaccctcc gtctttcaca cccgtaagct tttccgccaa gccgcccaga taaccgcgca
     2041 aaaccttggg cgcgagcgcg cgcaccgtat cgagcgcgtg ccccgtcatc atccccgcga
     2101 acacccccgc cgccgcgcac gtccacgcct gatccagcgg cacgaaccgc tccgccgcgg
     2161 ccgccgccgc gattgcagaa ataaaagcct ccagcacagc ctgcacgggg cggccgcgtt
     2221 cctgaaggct tgcccacacc gcgcccagca cgccgccggc cgccgcaaac agcagtccga
     2281 actgcaaaaa ccccattcac gacccctcat ccgccaaacc ccgcgacttt tgcgccgaaa
     2341 acaaaaacct caacgcatta ttccccgcca gcgcgcagaa aaacgccaac agcggcggaa
     2401 cgaccatacc cgtgttcaaa ggcgggtacg ccccccaaaa cgccaccgaa accaaaaacc
     2461 acacaaagcc cgaaaacaaa agcaggtaag ccgaagccac acaagccctc acgccccgcg
     2521 caaacagcaa tgccgtctga agcagccccg ccgccgccag cacccccacc gtccacgcct
     2581 ccggaatatc ctggaatttg taataaatcc gccacgcgta aatctcgtcg ggcgcaagcg
     2641 cgaacaccgc cgcgtaaccc gacaagccca aaccgctgac cgcctccagc acccgcgtcg
     2701 ccgtgccgaa caaccacgcc tgaaacccgg cgggcaaaaa ccgccgattc aacaaaaccc
     2761 tcaacatacc cgtcacacac cccccggcaa aacaccgcga agccgaagcc ccgcagtgtc
     2821 cgacgccgtc tgaaaactta ttccgcgtaa accggttgcg cgtctttggg gatgcccgcc
     2881 gcaccttcca acgccttgcg gtacacccaa ccggggtcgg gcgcggttat gtccgtttcc
     2941 tgaaaatcca atgtgcgcgc caacaggtag cgtttgcccg atttaaacgc attttccgac
     3001 acatagccgt tcaacgtcgc cgtaacataa ccgttgcggt aatccgcgcc gatatgttcg
     3061 acgacgtggt aggccgcact cgcgccggtc ataccgtcct cgactgcaaa attcaaggca
     3121 acaatctttt tatcttcaat catttcaaat ctccaaaagc ccgctagggg cggtaacgaa
     3181 aatgccgtcc gaggtcttca gagggcattg cccggacaaa acaggaaaaa ccgcagagcc
     3241 ggcatacggg ggcgtcagcc aacctgccaa acctcgtttt cgcgcgattc cggaatcagc
     3301 accctgatat tgcgcagctc gatatactgc caccagtgtt ccacaaaaaa cagcaggttg
     3361 gatttcggaa cggcagtatt aaactcctcc tgaaacagag aatactgttg gggcgcatga
     3421 ccccggtatt tcttaaccac tacaaactta tccgacctgt cgaacgcaag catcgtactc
     3481 cagtcggcat tggtaaaagg ccggtactcg aacgatatgc cgtagatatc gtccggcaac
     3541 tgaagcagcc cgccgtaagc attccaatgt ttatacatct gattttggta atcgtgtaca
     3601 cgccggtcgc cgcccaaata aacctgcttc ttactgtcgt accggaaact ttccgcaaag
     3661 gtgcgccaaa ccatcctccc gagcagctgc ttgtattcgt agtcggattg cgccaaatac
     3721 gaaaccgaca cgaacggcga aaacaccgat tcgtgggaag cgagcgacaa cttcaggctg
     3781 acgggtttgc ctgcggggat gacttgaatc tggtactcga ggcgggttct gtaccttgag
     3841 ccggattgtt cggtataatc cccgcccccc cttgcccggg ggggcaggtc ttcgtacctc
     3901 ccgaaagttc ggccgcgcca aatataattg gtatagttct cggtttccct gacttccctc
     3961 ggcgcaagca cacccccgtt caacagcagc tttgcctgca ccctgttgtt gccgaacgtt
     4021 tccgaaacca cataaaaatt cggaatcaaa accctcggca gcgggtcttt atccaaatcc
     4081 aacacccaaa cgttaccctc cgtccaacgc agcctgtgcg ccctcaacac gtcgccctcg
     4141 acggacgaag ccctgaccgt gccgttgaaa taacccgaat cggcctcaat ccgtccgcgt
     4201 attaccgcat tgtcggccgt caggatgccg tctgaagaca ccgtaaaatt acccccgccg
     4261 atattcaggc tgccgcccgt gatgcttccg agattggaag ataccgcgct caaatcggca
     4321 acctgaatct tatcggcagt caccgcgttt gccccgattt tatcggcggt aacggcggca
     4381 gccccgattt cccgcgccgt aacgcttccg gctttcaggc ggtttgcgtc cagcgtgtcc
     4441 gccgcgattt tgccgccgtg gatatccccg gcgttcaact tatcgacaac cgccttgccg
     4501 ttgaccacca attcgccgtt gatgcccacg cggtttttct gcgtgtccac cacgaacggg
     4561 aacacatccg ccttcccggc cgcaccgatg ccgaagcggt cggcattcac gataaacttg
     4621 ctttcgggcg tgccgttttt cggcgtagtc gccaagccga agcccgccac tttgccgttt
     4681 gcatcaacct tgaccgtgta ttgcgcctcc aagccgttga tgcttcgggc gtgtgtctga
     4741 accgccgccg cattgccgtc cgccgtcgat cggactgcct cgacgcgctc gttgacgctg
     4801 cccgcgccgt tgccgtcaat caggttgatt ttgtcgcgca aagccttgtt cagactgctt
     4861 tccgacaggt cggttgccga tacgtcgtaa acggtaaacg atatgctgcc gctgattttc
     4921 aggttatctt tgccgaaact gtcgtaaccc gccgccctca agtaataggt ctttcctttc
     4981 tccagcggtt tgccgccgca tttggcgatg gttataaacg tttccgcgcc gtcataggct
     5041 ttgtttgcgt ccgcggccgg gcaggctgcg ttttcgcaaa cccagacgat aatccctgca
     5101 aagtcttctt cgtcaggttt ttggcaggta aaaaacgcct ggcgcaggcc gctgtcgata
     5161 gagatgccct gcaatgcctg caattgcgga ttttgcgccg cgatttgcgc ccaattgccc
     5221 gttttaccgg taacggcacg cccgcgaacc ttgaagacaa taccgcgtac ctggccgccg
     5281 tccgccttca tatccgcctg agtgtaggtg tagctgttgt caacgatgcc gtcaaccgca
     5341 cgcagacggc gtcggctgcc gcctgcgtag atttccacgt cgtaggtatc cgcgccgtcc
     5401 aatttatccc aagcgatgac ggcttctttg ccgtatgccc acgatgatgt caggcgcagg
     5461 ttttggattt gaccgagcgg cgcgccttcg atggtgtagg aatacgccgg cacttccgcc
     5521 aaatcctgaa tgccgccgcc gaaaacgttg tacgaaacca gtttgaccca aaccgtgcgg
     5581 ccaatccagt tgcgcggcac ggcgtatttg aacaatgctt cgtcgatgcg cgcaaaccgg
     5641 ctgcccgccg cgtgtgcgtt gacggcggag ccgtacgcgc cgcgcgtcag gttgcccaat
     5701 gtgtagcgtc ccacgccctt cagttcggcg tcggcgtatg ccagaaattc gccgtcgacg
     5761 tagcacaatg tcagcaaatc gcggctgtcc tgctccgtgc cgcccgtcag ctgccccgcc
     5821 gaaatttcca cgcccaacgt atttgtgcgg tcgaaaaccg cgccgtcggg caaatcggcg
     5881 gtcagcgcgc cgaaacgcgc cttgcggttg accgcgccga cgcgggtgta gctgtcgccg
     5941 tccgccgata cccacacttc ggcgccgccc cacataccgc ccccggcggt tgccagccag
     6001 atttgcggtt cgccgcccgt cagttgcaac ggcgcttcga aaattaccgg cacatgggcg
     6061 ttgcccggcg aaacgttgta gtcggccgaa taacccgaag acggctgcgt cggatattcc
     6121 gacacggtat aaacaccggc cgggcagtct tcggccttga cggataaaac cccttctccg
     6181 tcttcttcga tttccgtgat tcggacgggc gttttattca ggccgagccc cgcgtcggtc
     6241 agggttacga tgtccatcgg ctcaagcagg cagtatttcc aacccagctt aaactcatat
     6301 tcgttgcgca cgtacagggc gcgttgcagc agttgttgcg ccactttttg cgccaccttc
     6361 gcatcgcaaa tgccgtgcat cctgaccgcg tctttcgggc gcaatccgta ctgctcgata
     6421 ttcgcctggt ctttcgcttc ggcgatggcg acgttgtagt cgttgtccct gtcaaggtac
     6481 tcgacctgaa tctgattaaa cgcatcggca ttggttttgc gcccggcctt tacagggtct
     6541 tgcgcgcccg aaacgatgaa atcgtcatcg gtcaggtcgt aggcggcctt attgtcggca
     6601 acgtacaccg cgccgttccc cgaatggctg ccgtccccgt aggggatgat tttcaggcgg
     6661 ccttgcgaaa ataccgccgc gctgttggtc tgctccagca gttcggaaat attccgttgc
     6721 gcctccccct gttccgtgta ggcagggctt aggaaaatcc cgacggcgcg gcaataattg
     6781 ctgtaccggt cggtatcgcc gatgttttgg gacgggaaac cgcagccgta gcgttggttc
     6841 gtcagcaaat ccaatacgat ttcgcgcggg tttgcatcgg ggatgttgcc ggaatagccc
     6901 gatttcccga tgacctcgaa attgtgctga tatatttgcg ccgatttcgt cagttcgtag
     6961 ttcgggctgc acaaataagc cgtgcccgaa taattcaagg cttggccttg gtgcttcgcc
     7021 tgttgcaggt gcgtccacaa cggctgctcg tcgccgccgc gcataagcgt caggcgcaac
     7081 tgtgccggcg aatcgaattt ctccttgtcg cgccaaaccc gccccacgcc ctgaatctcg
     7141 ccctcgcaca aagcaagcat gacggcggct tcgtaggtat aggaaatacc gacctgtttc
     7201 acaccgccgc cgcccttgcc gccccggcgc gtcgtagttt tatgctcgat ggtgacgaaa
     7261 tcgccgtacc acaccaaatt accggctaca cgcgtcctgc cgtagatgac gggcagggtc
     7321 agcccctgtg atgaccgctg aacctgtaac gataaaatcc gctcttcggc agatgtaacg
     7381 gttgacgatt taccacccat aaaaccacct cgaaacttgc aaaaattatc aaaatcgata
     7441 taatttactt atatcaaaac agatataaaa tatgaaaccg ttaaattttt caggcggttc
     7501 attggattgc ctgcggcaat tccccgaaaa cgccaaacag gcggcaggtt atcaacttca
     7561 agaaaagccg gaaaaccgcg ggcgccgatt cggaatcggc ggaaaaacgt tacaacaaat
     7621 tgattcggga aaagaaataa tggaaagcca aactttcgcc tccgtatttg acgcactgtg
     7681 cgacacgcct gcccaagccg ccaatatgcg gttgcgcgcc ggcctgatga tgcacatcgc
     7741 cgataccgtc cgcgaaaacg gttggacgca aaaacaggcc gcagaacatt gcggcctgac
     7801 ccggccgcgc atcaacgacc tgctgaacgg gaaaatcgac aaattttcat tggatgcgct
     7861 cgtaaacatc aatgccggac tcgggcagtg catttcctta tccttcgccc ccgcgtaaag
     7921 cccgtccttt cagacggccg caaaatcaaa atgaaaatgt aaaaaaccga accccgcgcc
     7981 cgataagttc ggcttggccg atgtcgtccg acaccacgcc gcgcccgatg tagctgtgaa
     8041 taaccttgcc gccgcccgcc aatatgccgc cgtgcgaaaa cgaccgcccg aaacgccata
     8101 ctgcaatgtc gcccgcctgc ggcgattccg tttcacggca aaattgcgtg acgaacccca
     8161 aataacgctc gcaatcgcgg tgcagatgcc aatcttgagg gtaagggcgc gggtcgaacc
     8221 cttcgggaag cagtccgacc gccccgtaga cggcgaccag gagcatcgcg caatcaacac
     8281 ccgcgccctt gaccattgca tgatgatggt acggcgtgcc aagccacgac cgcgcctctt
     8341 cgacgatccg cgccctcaaa tccgtttcgg acggcatatc acaccaccgt atctgccgaa
     8401 gggatgaacg ggaagccgcg gaaatgcacg atattgtcaa acttatcctt gcaggtatcg
     8461 cgccgcttgt cgcagcccgg ataaaccttg aacgcatcgc ccgcctgcgg cgggtagggc
     8521 aggcgaaggg caagttcgaa cgtgttgccg ccgtgtgcct tgaccgtcct gctcaaaccc
     8581 gcgttgcgcc cgcccgcaaa cttaatcacg ccctgcgaaa accagccgtc aggctgcgtc
     8641 agattgtgct tcagcacggt tcccgtccgg ctgttttcgg ttacgcgccc gtttaccgtg
     8701 aatttctcac ggttgacctt gcagccgtcg tcatagagcg tcctcatgca gcccgcctga
     8761 taaatgttgc gcgggctgga aacgttcaaa agctcgatgt cggatttcac gtcaaccttc
     8821 acggacgacc tgccgcccga tacgtccgac acgcgccctg aaaaaatatt caccgcaccg
     8881 acggggcgca gtccggcatc aaaaaacaca cggtctattt tgacccgcgc gccgtccaat
     8941 acgccgccca gggcggcctc cgcccattgc aggccctcaa gcctgtaatc gggcgcggct
     9001 gaaatctgca gggtgttgga atccacttcc aatccgacgg cggtacgggt tgcgccgcgc
     9061 ttgataacca gctcgtgcgc cccgtaggcc tgaccgtccc aaacgacggg catatccgcg
     9121 ccggtatgcc gcagcacccg gccgcccgaa agcgtaatgg tgtacaaatc cgccatctga
     9181 aactcgtcgc cgccgtgcag caaatcaatc agttcttttg tcgccgcctt catagtttca
     9241 cactcgtaaa ctcaatcttt ttggccgccc acaagctgcc caaaacgttt tcaaaatcca
     9301 ccgtgtcaga cgtaaacctc acgcggaaat aaaagccgcc cgtccatgta atcgggcggc
     9361 ccggcggttg cggcgtgttg aaaaccaaaa cgcccttgtc ggtaacggtg taatcgcgcc
     9421 cgtacgccaa cgccgtgccg ccgaccttga cggcgggccg ttccttgacc gccgacacag
     9481 gctcgataaa tccgcccatc gaacggacaa gctgataacg cgcaacgccc tgcaccgtgt
     9541 ttccgacagg ctggtcggtt acggcgttgt cggccgggtc ttcgtaaagg aaactttcaa
     9601 agctgccttt gcgcgcgttg aagaatcccg ccagtttttc caactcgttt acggacgctt
     9661 ttgtccgcaa tacctcgaac gacagcgaaa accgccattg cgggtaggtg tagtaggcgg
     9721 ttcggaactc acggccgctt gccgatttct gcgtccccgt actccatacc gccgttttct
     9781 tccgccccca cttcaagccg gggaactcgg ggaaaaccgc attgcccatc agatgattcc
     9841 tttcgctttg agcagggcgt caaattcgtc ttcggacagt tcgccgccgc caagcatacc
     9901 gacggcttcg gcttcgtccg cttcgctttg cacactgccc gacgacggct tgacgcccat
     9961 atacgacgct gccaagatat gcacgggcgg atgttcgcgc caatactcgt tcaggtgttt
    10021 gatacgcggc aaatccaagt tgtcggcgac gtagtcccac gtccagccgg tagaggcgca
    10081 aacgtgggca atcaccgcgc cgaaacttaa tccgccgccc tggcctcccc cgcttgtgcg
    10141 gcttcctgct ccttgcgttt caagcccgaa acgtccatca cggcggcaaa cacttcgttc
    10201 atgttgccga tatcgatcaa atcggccgct tcttcgcgcg ttaaatccgg gtaattgcgc
    10261 ttcagggcgg cgtgggcgca atcgataacg gtagagattt gtccggcatc cgccgcattg
    10321 ccgtcaaatg agccgatgcg ctcctgcaac tgctccaacg cgcccagcgc aataggcgga
    10381 atcacataat ccgcgccgtt cagttctacg gttacgcctt taatccgtac ggtcattttt
    10441 atttccttga ctttggttga ataaaaaaag ccgccttttc agacggcata taaacattac
    10501 tcctgaatcc acagcgtacc gattttaaag cccgcctcgt cggttgaggc ggtaaagtcg
    10561 atttcgggga cggagaagtc gtcgttttta gtcgagaaca agcccaattt gccgctggtt
    10621 acgctttcca gttccaacag ggcttttttg cctttgaact gcgtcaggta cctcatctta
    10681 aacgtcggcg tgttgcccgt cgccatattg gtcagctcga ttttcttcgc cgggggcatc
    10741 gtttgcgtat aggtaaagct cggataaacg gttttgcctt tatccgagct gttaaaggcg
    10801 taaacgcccg attccgaaac cgtatattgg ccttgtttcg gggaggatgc tactttgata
    10861 taagccgtgc cgtcctggcc catcacgccc gcgtcctcag cgaacttgcc gccgccgggc
    10921 gcggctgcct gaaggtaaga ataattgtcg ccatcgagga ctttgcccgt agtttccgcc
    10981 caaagcgcct tcatcgtgcc ggttgcatat tccgtaccga aaaacagggt attgagggtc
    11041 aggccgttaa tcaacgcgcc tttcattttg cccgacacct tgaccttgcc ttgcgccaca
    11101 gccaaaggat aacggttttg accgtagaac tctttcaatt ccgccgacaa atcgacggac
    11161 atttcctgca agcccataat ccgcacgggc gttgcgttct gtacacggtt gccgtaagca
    11221 tccgtaatca tttgggcgaa cacttcgccg ctgccaaacg tcaactgcat gacatccctt
    11281 tcaaaaaaag ccgcattacg cggcgcaaat cacaatcgga ataatacaaa ccgcctgttc
    11341 gccaagcgtc ccctcgtcgg tttccaccgt accctcgacg cggcaatact cgatatccgc
    11401 gccgtccgcc gttaaatccg tcttgcccgt aacggggtgg acggcgttca cggcattgca
    11461 caccgcgtca atcagcggat tcatgacggg cgcgggcggt tggcccgccg tctggacgta
    11521 caggtaaaca tcggcgcgca aaatccactt ggtctcccgc cccgtcaatg tcaacgcctg
    11581 catatcgccc tgcgccataa ataacgcggg ctgatcgtag cgtttcacat cgttccagtg
    11641 caccagcttc cggctcttgg ttacgaagcc gtccaatgcg tccagcttcg cccacagcgc
    11701 ggaataaacc gcctcacgat tcatttcaac acctttccaa tcgatttttg caggtcgtcc
    11761 gcgaacttcg gcgtcaaatc gcgcaatgcc gaacggagga acgaccgctc cggcaacttc
    11821 acatcacggg tatgggcgcg gacgcggaca tatcgcggcg gtttcagcgg cttgccgaac
    11881 gcctgcctga cctggcgcag cgaagcctta acgttgacac tgcccgcgaa accatactca
    11941 tgcgcgatgc cgtaacggac gttcgtatcg acttcgcccg aaacgacatt gccggaaacg
    12001 ttcacgcgct ggtgtacgga gcgtcgcagg ttccccgtcc ttaccctcag cacccggccc
    12061 gataaccggt tctgcttgac ttcgcgctgc aggcgcaatg ccgacctgcc caccgatttg
    12121 acgaccgcat cttgaacatc ggcggcatat gctttaaaaa ccgccgccag aataccgccg
    12181 ccgataaatt ccatcttcag catcacacgc cctttcgttt gtactcattg agtatcgcaa
    12241 acgccgacgg cggcataccg ccggaattac cgaacgtaga aaaagcgacg gtttcgcctg
    12301 caagcgtctt gctccgtacg cccttgccct cgatttcgtt cacgcgctgc accgcgataa
    12361 tcatcaccgc ttcgcgtatg tcggcgggca tgcgctcata accggcgcgg tacgatacct
    12421 cgacattttt cagcccccgc gcgaaacctt cggggcgcat cagcagccag ttgtcaaact
    12481 cccagccgcc ggcatccgcg ccgttgattt tgacggacga caccgacagg acgggataat
    12541 tgtccaacac gatgcggtct ttgccgttgc cgtcgtaccg cccgacataa tcggcggcca
    12601 ggaggctgcg cccgatatag gcttccgccg ccgccgacac gccgtcaatg acgctgagga
    12661 agaaatcgtc ccgccggtca tgttcaacgc cgatacgctg cttgaactcc tcaagcgata
    12721 ccggggcggc catcgttatt cagccttttc ggcttcggca ggttcggctt ctgcttcggc
    12781 aggttcggca gcttcggcag gttcggattg tcctgccggc tgcccggttt taggtttacg
    12841 cccgcgcttg gctttttcag gcccttcggc aggctcggag gcaacgttgc caaaaccgaa
    12901 ctgatacagg aattccgccg cctccgacgg cacttccacg ataccgtttt catctgccgc
    12961 gtagctttgg ctgccgaagg aaacgtcggt aaacccttcg ggcgctttta atttgactgt
    13021 catctcaaaa tctccaaaag aaaaagccgt ctgattttca gacggcatat cggcttaacc
    13081 cacgttggta atcataccga aggcaggcat gaacatacct tgcagcacct cgtccgcata
    13141 gacgccgtat tcgtacatac gggtgcgcag cggccattcg atttggtaat actcttggcg
    13201 cgtacgcact tgcagcagat tgccgacgcc ctgaacgtag gcgggcaggc ggcttgagta
    13261 gaacaggtaa gtaccggcag gcaggttcgg gtgtaccacg atgttcaggt cgtcgcccgt
    13321 gatcttgttc atatacgaac cgacaacgac accggcgcgg atgttcgccg tattgttcac
    13381 gtcaacgttc aacttaatca acggcgcgcc gctgtttccg ataatcagct tggtcagcgc
    13441 cgccaaatcg cgggcgttga cgtagacggt atcgggggac aggcggtatc gggtaaagaa
    13501 atgcgcgaac gcctcttcaa attcatacac gccgcccgcg ttgtcggaag tcaggccgcc
    13561 gcctttgttg tccgaccaga acgcgcccga atcaggcagg gcgatttggg tcaacaggcc
    13621 gtcaaattcc aaaatagaag tggaattgtc ttcggacggc agggaagcgg cggtctgggt
    13681 accttcggca tcagccgaaa tttccacttt ggcggcagtg gtaaccgcgc ccagtttttc
    13741 agaaccggcc gcaccccagt accaagcata ggcaaccgcg ccgcgaacag ccggaatcac
    13801 ggcggttact tttttgcctg cctcaatacc ggaaacggaa gccgccgcag attttcgggc
    13861 ggaaccgccg ccgaacgtat cggttgtacc atccgcgttt cggcgtgtga ttttggcagg
    13921 gacttgggca gttttaatgt tcgggctttg tccggttgca ccgttgttcg cgcctgccac
    13981 gtcccagtat gcctgcaagc ccaaagccac acagattaca gacaaggtgc tgacgctgat
    14041 tttccccgcc gcgtcggcgg aaacgacggc ggtcggtgta ggtgtaacgc ctgctttcag
    14101 gctggtgttg ccgcccagca aaatcatttc ttcggcaacc atagtagcct gaagggtttg
    14161 ggcgaccgcc aacgctttca cgtcctcgaa accgcgcgcg gcgtaatccg cttcaaaggt
    14221 tacttggttt tccaagccga tggcgcggaa ttgcgcgttg cgttccaccg tttcgtgatt
    14281 gatgacgccg ccgcgcctgc cttcgccgat accggcgcgt tggttgccga cgttgatatt
    14341 cgtgacggct ttccagttcg agccgatggt gcggccgccg cccacgcgcg ggatacggtt
    14401 gcgcaacggg gtcaataccg ggtagagttt ttgagacggc gcggaaaggt cataggtttg
    14461 caggccggcg gtaaagccgg ccggctgggt aaaacctttg tcaggcggcc cgccgcccgc
    14521 ttgtgccgac ttcatcagtt caatcgtttc ttgtgtgagt tggttcacgt tcatttatcg
    14581 ctcctgataa taaaaaaacc gcctgtaagc ggtgttacag acggcctgtt tgttttgcct
    14641 taatgagtgt cgccacgtca tccagcgtgc cgtcattctt tacaatcggc tgaaaacctt
    14701 ttaacgggtc ttcgccgtta tcttctgcct tactgatagc tttcgtactg cctttcggcg
    14761 gtactgcctg tttcttcagg ctttcgattt ccgcctgtgc tttggcaagg gcgtcattcg
    14821 attttttcag cgcgtcttgt gcctttgcca gttcgtcagc cgattccgct ttggcaagat
    14881 cgcctgattt gccggctttg gccgccaaac cgtcggccgg cttatcggct tctcttgccg
    14941 ccaacgcttt cagcgattcg gcaaggccgg ccgccgattc tttgatttgc gcggtaacgg
    15001 cttcatcgat gttgtcgtag gatgcgtcct caaccagcca tttcagcgac atcaatacat
    15061 cagccggtga tttgacttgg tacattgatt tggcggccgt cccgcctttc ggcttgtcgg
    15121 ctttagccaa taccgctttc aaggcggcga tttcagattc ggacaattcg acgcttgccg
    15181 attttccggc ttcgtccttt ttgccggcgt ccttatcgcc tttatcttcg gttttaggcc
    15241 ctttatcgcc gtctttagga ttttcatctt cctcttcggt tggtttgtca gacggcttgc
    15301 cgtctttatc cgccgcccct gcctcatctt tcggtttgtc cgccttaaag caggtaaaca
    15361 ccgcgtcggg attggcaggg cggtcgacaa ggctgatttc cgtcagcttc aagcccgtga
    15421 tttgcgactt gttcaaatca tcgcgggcgg taaccctgcc gccgatggaa aagcctttgt
    15481 aaacgcctgt cttgactttc gtcaccgcaa cagggtcaac gatatgcgcc ccgaaaaatg
    15541 tgcgcccgtc gtcttccacg ttgatttcaa tcgccgttcc cgccgcgttt gagccgtgca
    15601 tctcgcgcac cgcgccgaac ttcatataat cgggaatcgc cgccttcatt gcttccgccg
    15661 cgacgacttc gccgtccgaa tcgaccgctt cgctcgaggc atagccccaa actttgaccg
    15721 tgccgtcgtc ctgcgcctcc gtcttggcga tttgcgcgta taacttcgtc attggtttgc
    15781 tccaaaaaaa agccgccccg ctaaggggcg gcaaaacaca cccgcctgac ccgaaggaat
    15841 caagattcag gcatatcccc tgccaaaaca ggcacaaccg cgcaccggca gttcggatga
    15901 ccgggtatcg tcaacgcacc gtgggagaaa ggctcgtgca aaccgattac gcccatcccg
    15961 ccgttggcat tgcagacatc ggacaccttg tcgtcttttg cggttatcca ctgcttgccg
    16021 gcaaccagcc ccgttccttc ccagcctatc aggttgccca taccgtccgc catcgccgtt
    16081 tccgttcggg caatggttcg ggcgcgtgcg ttgccgaaag catgggattc tttcaggcgg
    16141 cctgccaatt cctgcacact gtcgccgttt cgcatggctt cggctacttg ggcgcgtatc
    16201 atttcgcgcg tcccttcggt gatttgccac tcggcggcag gatttcggac aagcccgccg
    16261 cccgcccgct tcatgccgac catttcggcg gcgcgttcat gcgcccactt gacggcgcgg
    16321 ctgcgaatgt tcgtaaccat accggcggca ggttcaggca ttacgcgcaa caaggcggca
    16381 accgccccgt cttccgcaac acgcctgatt atcggctcga ccacatcgga caatcccgac
    16441 caatcgccaa aatccaagcc gtttgcaaca acccctgccg cgcggctcaa ttccgccgcc
    16501 aaatcctcag cctgccaatc gacggccgca ctatcaatca gtgccgcgat ttgttcagcc
    16561 aagccgtcaa tgcgtgtcag caaataagcc tcaataagcg cggcagattc gtcttcgctc
    16621 atcgggcttt ccgactttcc cagcttttca gcctcttggt tcggctgctt ttcaggcttc
    16681 caatcgtctt gccgattcgg ttcaggctcc tcctgatccg gtaatggctc tttacccagt
    16741 tcggcgcgga tttcatcagc ggtcaagatg cctgcgtttt tataaatggc gtagatttcc
    16801 gcctgttctt tcgggttgag tgattcctct tccttccaga caaactcata cgccgccata
    16861 ttcatgtatc gggcaagcac atcatcaatc agggctttta cccagttttt caggctgctc
    16921 atgccgtctg aaagcgactg ctcacggctc gtctctgcca cgcttcggtt tacctgcgcc
    16981 acgaacggcg taggctcgac actaaacgca aagcagacga cacgcgccag ccattcgtcg
    17041 taaacgtcct taagcggcgg ctgcttcgtc tctttgaagt ttctagccaa ctcacccgga
    17101 acataacgca gcttgcgccg ctccgccgtc tcgcctgaca acagcaaatc aaagtattct
    17161 tggaagcgtt taatatcttc catcgcccac gtttcaggca cgccgatcaa agcatcggga
    17221 acactgcccg ccgtgtaata ttcaagcgcg tgaatctgcc gttttaaggc aatattcacg
    17281 gtcatgatga tttgctcgac aggtgaatag ccataaacct tgtaacttcg gttatttcgt
    17341 gagcggtaaa tcaattcgtc cgccgtatag tcaactgccg ccatgccgtg caagatttgc
    17401 tgataagctg tatcaggcgg caatggcaga cggcctgtat tgtccagtac gcgtttaatc
    17461 gtcgcaccgt ctatcacttc aagggcgtac aagtcgccgc ccagtgtttt acgtgggtag
    17521 atacacggcg cgtcaataac aaacaggtct tccagcaaga tgcgcaacca atcagcccat
    17581 gtgtgctctt tgtcaggcga ctggaagaac gcaatggctt catcgacctt tctgtcttta
    17641 cgctgcgatt cgttgtcttt ggttgattcg acatcgcgct tttggattgt ccacttaagg
    17701 cactccattt ggtctttacg cgtctcgata accaaacgca gcacatcgta gttatcggca
    17761 agggcgcgta attgtgcaaa gcctaccgct tcacgttcgc gcggtttgga atgcccgacg
    17821 ttgtagaacg gctcgtaatc aaaccgccgc ccctctgcct gctgcgcgac aggggctaaa
    17881 ggctcgcccg cgtcaaacca cccgtccgca ttgccggtaa aggcgtaacg gacaccggca
    17941 gccacacggg caataaaacc ttgtgataaa ggtgtctttt tactcatttc atagcctcaa
    18001 cctgcgattg caggaaatca atcatgcccg ttcgggtatc cagtagctcg ccaaacgcac
    18061 ggctcaaaca gtcgatttgg tcgtcatgct gaccgtttgg gaacatccgc atttccgcaa
    18121 tcagcgcgtc tgtgccccat gtgccgtcat ccaacaccat cacattgccg atgttgacct
    18181 gtgccgcgaa cggttcggcg cgtgtaacct tgtcgcccga ttcaggactg gcagatacag
    18241 aaaaacccgc caactggcgg gttagataca gggtttgcga tttaccagcc tgcccggggt
    18301 cttgtgggat ggatattttc gttttcacgc cgtctttttg cgccgtgttg cgcaatatcc
    18361 tgtccctttc atccgcgccg tactgaccgc gcacgatatt ggcaatgatg taccgaccgt
    18421 cttctgtaac gccaagcctg ccgcctgccg tgtagtcgcc gtcgttcgca gtggatgcca
    18481 agtcccacgc gcgaacccat ctgatatttc cagcgggcag ggctttcaca aattgcaggt
    18541 tgtcaggctt gaacgtaccg ccgtcaggcg gcgcaggttt ttgcaaatac tgcccggcaa
    18601 acacatacgg cgcggcttgt tccatacggt gcaatgtttc aatatcatgc ttttcaggcc
    18661 acaacgccgt gccgtcgtct tgaatagctg gcaggcaaag gtgttcccac tcttcgccgt
    18721 tgccgccgtc aagcagccag cccgccaagt ctttctcgtg caggcgttgc ataatcagaa
    18781 taataggcgt gtcagggcta ttcttccgag attcgaccgt gttttgaaac cagtcaatga
    18841 cgttctgccg cctgacctcg cttcgcgctt catcagcttt gtgcggatcg tcaatgatga
    18901 tgcagccgcc gaatccctcc cgatgcttgc ctgcaccgaa acctgtaatc gtaccgccag
    18961 cacctgttgc gtacatcaca ccgcctgcgg ttgttttcca gtgatggccg ctttcgcctg
    19021 ccagtgccaa atcaggaaaa atcgcccgat actcttcgtg ttgcaccaag ttccgaatct
    19081 gtacggagtt attgaccgcc agcgccgccg aatagctcgc gtggataaac tcgcaatcag
    19141 gcacgcgccc catcgcccac gcgataaagt tcacgaccgc gatttccgtt ttcgagtatc
    19201 gcggcggaat attgataatc aggcgtttcg tttcgccgtt gaaaacacgc tcgagcgcgt
    19261 tgcagattgg cgcatggtga ttcgcccgct gccaaacata gccgcgcctt tgataaaaca
    19321 tccggcgcgt gaacatatac aggcttgcag aacttaaact gcgaattact gatgtttcaa
    19381 catcgtcgaa ttgccctagt gccatttcat tctaacttcc tttggagaaa cggccataca
    19441 gcccctctta tgattagcgt tttatgctaa accctacgca acacatcttc cgctatcttg
    19501 cgatattcct cagcatcaag ccgtacagcc gccctcatgc tcccatcgcc cgatttaacg
    19561 tcaagttccg tcttatcgcc gtatttcttc ggcgcaatct tggaagccgc ccactttcgg
    19621 gcatctatct gcaatttcgc ctttgaaacc gccgcgctct ctgcttgtgc actatcggca
    19681 atctcgatga tttcttcagc gaaatagtcc gcctgctttt ctctcgcgcg cgcgtattgg
    19741 tggcgaaatt cttcattagc ttccaaccaa cggtaaatag ttttcattgg cggcacacca
    19801 tcttcggcgc atattgaacg taggcttctg ccatttgcga ttttttcgca tatcttatcg
    19861 gccatgtctt tcgtgtaatc tgtcggacgg cctaatttgc gtttcgtgcc gctcataaac
    19921 cctcctcaaa aaagaaaccg tctaactctg acccctctca gaattagacg gcaaacacac
    19981 actcgccaca taggaaaaac ggaacgccgc tacctgtaca ggcagaagct caaattcgga
    20041 cagcctaaaa cgcaaaaacc cgcacattgt tatgtacggg cttaaaaatt catatccttc
    20101 gggcgtgcga aaatccccgc aagggtaacg atttgaatta tacacctatt gccggaaaaa
    20161 acaacaggcc gtctgaagat tcagacggca tttgtattcc ctactgcatc aaaccgccga
    20221 caggttgcgg atttcgggca gtatcgggcg gattttttct gcgtgttccg cgtcggcgtg
    20281 tgcgcttaag gcttcgaggg cgtttgcggc ggctttgagg cggctgcgcg tttccgccca
    20341 gaccgtccac atcgtaaccg cctgcctgca gccgagctgc ttcagcggca gggaaacgtc
    20401 tctgcccatt tggatggccc gcgcgccgta gctgacggcg acggcgaggt cgtacagggc
    20461 gttgccgctt atgggcaggg cgggttgcgg cgcgggtaac ggttcgcggt cggggacttc
    20521 gccggccaaa cccgtgtgca gtgtcaatgc gtggacgtag gcgacggctt cgggcagctt
    20581 cccggcgggg atgtcttcga tggcttcgac gttgaagcgt tggtgtacca tactgtacgc
    20641 tgaggagtag tctatgcctt tgcgtccgac aagtgcggca acggcttggc gcaatccggt
    20701 acggtcgtcg gcggtggttt tttgtccgac ttggtagccg cctgttttgc ggatggtggg
    20761 caggacttcg gatgttaccc atttgcggaa tttccaagcg gttgagcctt gttccattgc
    20821 tttacggctg cgaagaatca aaatatataa accgctttcg ttgatgatgt tcacgttacc
    20881 gccgcctccg tgaatttcag accgccctat gttaaacata gaccgctcgt catcatctaa
    20941 tttttcaagt gcttgcgttg ggttctggat ttctaaagcc ttacatacat cggcggcaac
    21001 aaaccaagtc aagccgtctt tttcaaaggc acgaacggga gaagtagtat tgaaattaaa
    21061 tgattgaatt acgttcataa tgaagtttcc tagtgagttt tcttaatgcc cgttagggcg
    21121 gacgcgtggt taagaaccct cactagatgg gcggacttat tccccttacg ggtattgtat
    21181 tcgtcgccca cgcgtccata agaaacttcg gttgtgccat cgaaacaaac aacacgaaag
    21241 gaaacttaca gatatgaaaa aatcacattg acggagtgat tgccgctagt gtgtggttct
    21301 tacgccacga acaggaatat aaaacaaacc cccctgcaca tgcaaggggt ttcgttacct
    21361 tatttgggtt tgcgtttaaa attctcgtca tcacaacaga atcggtaaga atcaataacc
    21421 aaccaagcaa cgcgaagcag attttcaggc gtatcaataa taacttgatt gcctgatacc
    21481 tcaagtccgc atcgttcgat attcgaaata tccgactctt ccaattcgat agggaagatt
    21541 acagacggcc tttgtttgtt gtcaaaatac cgtaatatcc acctgttact tttaccatca
    21601 accaatacgc cgaaatagct ttccgtatct tttgcctcaa tactcgcatc atcaggaaga
    21661 attgatttaa ccaaatcaaa caaccttctt tctgaataag tggtaacgat tttattgttt
    21721 tcggggtcga taatcggcgc ggtcgggtct tcctgctcct tctcaacagg ggcggcctcc
    21781 tcctgcactt tcggcgcact cagtcccgat acaaccattg aactaaccgt attttgaaca
    21841 gcctgtttga cgatatgacg gatgctttcc aaatatcgtt gggtaaactg ccgttgaata
    21901 ttcgcccttc ccgccacata acggacgaaa tccaaatcca cctctttcaa gctttctgta
    21961 attgattctg taaatgccgt caggtatatg ctttcttccg caaggcttcg caatgcatca
    22021 ggttgaaact tgtcgtgcct gaactgatac agttgcgcca tatcattttc attcaacatc
    22081 gtaacatcaa cggtcaaaaa cggctccgaa tccataatat tcttgttgga caaatcggta
    22141 aaaaatcgcc attcccggcc gttggtaatc gcgcaaatgg ctatttcagg cgtggcattg
    22201 aagtaacgcg acaactgcgg acaatgattg gacaaatttt cagtatatga ttttgcttcg
    22261 ataaacataa ccggtgcgcc gttgcaaaac aatgcgtaat cgacccgctc ccctgatttt
    22321 gcacctggga aatccgcctg gtattccgcc cgaaccttat tcggatcgaa tgccgaaaaa
    22381 cctagaatat ccaataaagg caaaatcaaa gcctgttttg tcgtctcctc ggtagtacag
    22441 atatgtgcaa ccttcttgac atgttccgca tgggaagcaa tccgttcttt aaataccgca
    22501 cttacagccg ccgcattcat gttaatctcc ttggttaaat ttgattgggc aaattcccac
    22561 cccattaaga agtgttaatt ttcgcaatca tagcgcaacc gaaaagaaaa tcaaaccttc
    22621 tcaaacagca aatcaaaatc attccctgcc gcctgccgta tcgccctata ccacgcggcc
    22681 aagcccaaat ccgtctgcaa atgcaggggc ccctcgccgc gccgcctgat ttcagcctgc
    22741 aaacgttcct cgtaggctgc ctgcgacttc gcaccgatgc cgaacgaaat acggacggcc
    22801 tcttgttgcg gcgcatccac cttcgcccat gcctgcaaag taagaaacat ggcatcttcg
    22861 ccgtatctca agccgatttc aggcttatgc gggcaagctt cctcccccat ataacggcct
    22921 tcgatactca aacatctatt taggctgcgc gtatcacggt aacgacgctc aaaagcacgc
    22981 gccaagtcgt tcataaattc aaattcttgc tgattcatag cttgattaac cctttttcat
    23041 gcaacaaaac caaagtccgc attacacctt ccgcgaaggc cgtctgaagt tccccttccg
    23101 cacaatccgc caagcggttc tatacatcaa aactcccaaa tgattccaaa ttctaccgca
    23161 gcccgcgcct gcaccgtttc ggatatggcg gacctcggca ggaacgccga accgcccgca
    23221 attgcggcaa acaatacaac ctatatccgc cactttttgc agatgtgatt tttccgcttc
    23281 ggttttactt ttcatccaac tcccccacct ccaccgccaa accgccgcct gcgaccggct
    23341 cgtcggcgta atcggcggca atgcgccgca cctgcctgtc gttgtgatag gcaacgcctt
    23401 gaagcgcgtc cagcgcaacc ttcagggcgt tgtccaaatc gatcaccgtc ttgtttgcgc
    23461 cgccgtcttt gttcgctttg ggaatcagcc gcacatatac ggcaaccgcg ccttcggacg
    23521 gcatcgcgcc cgcttcttgc gcgatacggc ggacgacgga cttatacgcc gccgcctccg
    23581 cgctcctgac cgccctgttg cgccaaatcc gccaatatcg gtttgccgat acggggtaag
    23641 gcaggataag gcgtatcacg cgcattcccc tgccggttgc gtccacaggc cggcgcccaa
    23701 tatcgggcag gcggcgtgcg cggcgcggat gtatccgtgc agccttgccg ccgtcatcgc
    23761 gccggctccc gccgccgcgc gtttttccgc gcgcttttgc cggactgcct tgttgcggca
    23821 ggttttgcac gatttgtaat aatagacccc cccttccttg cgcgggacgg cattaaaccc
    23881 tttctccaac ggcttggtcc cgccgcaggc cttgcaggtg cgcatttctt ccgccatttt
    23941 gatttttcct tttgttttta tgtgtttaat atccgaagcc gtcgaagtcg tcttcgcctt
    24001 tgccgtcgcg cttgcggcgc aaaaccgcgt cggaggcttc caccaaaagc cataccgcca
    24061 acgtcgccgc cccggcaaac gacagcgcgg tgatgatgct taacagggta tccattgttt
    24121 tatttccttt cggtcggttt tatgggtcgg ggtcggattc ccgccgcctc cgtcattccc
    24181 tcgcaggcgg gaatccggac cttggaacaa cagcaatatt caaagattat ctgaaagtcc
    24241 gggattccgg attcccgctt tcgcgggaat cacggcggtc gggttggcgg gtcaaatgcc
    24301 tccgcccgcc gtttcaggcg gcatcgggct ttcagggtgc ggggcccgcc ccgcttcagg
    24361 cggcattttt tccaacaaat cttcgacaca ggcggccgcc cacgcaaaat tgtcttcgcc
    24421 gtcgcggatg taataagcgg cggagatgac ggcaagcagc gcggcctccg tgccgccgtc
    24481 gaacccgcaa tctttcagac cggcg
//
LOCUS       DAWTQS010000026        23725 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.026, whole genome shotgun sequence.
ACCESSION   DAWTQS010000026 DAWTQS010000000
VERSION     DAWTQS010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23725
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.026"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(<1..242)
                     /locus_tag="WNA25_001047"
     CDS             complement(<1..242)
                     /locus_tag="WNA25_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823814.1"
                     /translation="MPSERLSDGIFPCILNRVYETPDAFGGNVGCCAMFGGDQRTKCA
                     GKDLMPSEGSDGIGIGESEAVAHAQRGFVGFEAGVF"
     gene            complement(260..817)
                     /locus_tag="WNA25_001048"
     CDS             complement(260..817)
                     /locus_tag="WNA25_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFA9823815.1"
                     /translation="MDALKLLTNRRSSKKLKHPAPDAAELEQILQAATQVSDHGNMRP
                     FRFTVIQGEVGLQRFRDVLKQTVAELNFGDDAMKKAEKVGNMAPMVIGVTFVPNRDVP
                     KPKPEWEQMLTAGCAAYALQLAATAQGFDNVWITGMWVNSPLLREAFGCEDKDKIIGL
                     MMVGTPTEEVHKPKNTDLEAFVSHW"
     gene            complement(892..2526)
                     /locus_tag="WNA25_001049"
     CDS             complement(892..2526)
                     /locus_tag="WNA25_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase LptA"
                     /protein_id="HFA9823816.1"
                     /translation="MIKPNLRPKLGSSALIAFLSLYSSLVLNYAFFAKVVELRPFNDT
                     GADIFLYTMPVVLFFLSNFVFHVIALPFVHKVLIPLILVISAAVSYQEIFFNIYFNKS
                     MLNNVLQTTAAESARLITPGYVLWIVCLGVLPALAYIAVKVKYRVWYKELLTRLVLAA
                     VSFLCALGIAMLQYQDYASFFRNNKSVTHLIVPSNFIGAGVSKYKDWKRSNIPYTQLD
                     MAVVQNRPAGSLRRFVVLVVGETTRAANWGLNGYSRQTTPLLAARGDEIVNFPQVRSC
                     GTSTAHSLPCMFSTFDRTDYDEIKAEHQDNLLDIVQRAGVEVTWLENDSGCKGVCGKV
                     PNTDVTSLNLPEYCRNGECLDNILLTKFDEALNKNDKDAVLILHTIGSHGPTYYERYT
                     EAERKFTPTCDTNEIDKCARATLVNTYDNTVLYVDQFIDKVIRKLENRDDLESAVYYV
                     SDHGESLGENGMYLHAAPYAIAPSGQTHIPMVMWFSKAFRQHGGIDFQCLKQKAAENE
                     YSHDHYFSTVLGLMDISNSQTYRKEMDILAACRRPR"
     gene            complement(2924..4030)
                     /gene="serC"
                     /locus_tag="WNA25_001050"
     CDS             complement(2924..4030)
                     /gene="serC"
                     /locus_tag="WNA25_001050"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HFA9823817.1"
                     /translation="MSLYPIYNFSAGPAVLPEAVLRTAQQEMSDYNGTGFSVMEMSHR
                     SEMFLSILHHAEQDLRQLLKVPDNYKILFLQGGATTQFNMAAMNLAHGFRTADAVVTG
                     NWSRIAYEQMSRLTDTEIRLAAHGGEQFDYLDLPPVETWDVAPDSAFVHFAVNETVNG
                     LQYREVPRLSDGMPPLVCDMSSEILSREFDVADYGLIYAGAQKNIGPAGVTVVIVRED
                     LLERCPNDIPDVFNYRSHLNRDGMYNTPSTYAIYMSGLVFRWLQAQGGVKKIEAVNRL
                     KAQTLYETIDGSGGFYINDIHPDARSKMNVVFKTASEDLDRRFVLEAELQGLCLLKGY
                     KSVGGMRASIYNAMPLEGVRALADFMRDFQRRYG"
     gene            4396..4827
                     /gene="rimP"
                     /locus_tag="WNA25_001051"
     CDS             4396..4827
                     /gene="rimP"
                     /locus_tag="WNA25_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFA9823818.1"
                     /translation="MDIQTILEKTLPGLGYELVDFELAAQGTLRVFIDKEGGITVEDC
                     ATVSNHLSRVFMVEDIGYKNLEISSPGLDRPLKKAADFVRFAGQNAKIKTRLPIGGQK
                     NFIGKIEGCENDTVTVSFDGKTVQIELGNIDKARLRPEFKF"
     gene            4855..6357
                     /gene="nusA"
                     /locus_tag="WNA25_001052"
     CDS             4855..6357
                     /gene="nusA"
                     /locus_tag="WNA25_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HFA9823819.1"
                     /translation="MSREMLQLAEALASEKNVDAEVVFQALEFALSTAAKKKADREHM
                     DVRVQINRDTGEYQTFRRWLIVADEDYTYPDVEKTIEEIQEEIPDTTIQIGEYYEEQL
                     PNEGFGRQAAQTAKQIILQRIRDAEREQNLNEFLAVKEDIVSGTVKRVERHGIIVEVV
                     AGKLDALIPRDQMIPRENFRSGDRIRALFLRVEEIGNTGRKQVILSRTSGDFLVKLYA
                     NEVPEIAGGMLEIRAVARDPGQRAKVAVKANDQRIDPQGTCIGVRGSRVNAVSNELSG
                     ERIDVVLWSPEPAQFVMSALSPAEVSRIVIDEDKHAVDVIVAEDRLALAIGRGGQNVR
                     LASDLTGWQLNIMTSAEADERNAAEDAAIRRLFMNHLNVDEETADVLVQEGFATLEEV
                     AYVPAAELLAIEGFDEEIVDMLRNRARDAILTMAIAAEEKLGEVSDDMRNLEGVDADM
                     LLSLAEAGITTRDDLAELAVDELIEITGVNEETAKAVILTAREHWFTEDK"
     gene            6369..9200
                     /gene="infB"
                     /locus_tag="WNA25_001053"
     CDS             6369..9200
                     /gene="infB"
                     /locus_tag="WNA25_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFA9823820.1"
                     /translation="MSNTTVEQFAAELKRPVEDLLKQLKEAGVSKNSGSDSLTLDDKQ
                     LLNAYLTKKNGSNGGTISIRRTKTEVSTVDGVKVETRKRGRTVNIPSAEELAAQVKAA
                     QTQAAPVQPEQTAEDAVKARAEAAARAEARAKAEAEAAKLKAAKAGNKAKPAAQKPTE
                     AKAETAPVAAETKPAEPKEKAVKPKHERNGKGKDAKKPAKPAAPAVPQPVVSAEEQAQ
                     RDEEARRAAALRAHQEALLKEKQERQARREAMKQQAEQQAKAAQEAKTGRQRPAKPAE
                     KPQAAAPAVENKPVNPAKAKKEDRRNRDDEGQGRNAKGKGAKGGRDRNNARNGGDERV
                     RGGKKGKKLKLEPNQHAFQAPTEPIVHEVLVPETITVADLAHKMAVKGVEVVKALMKM
                     GMMVTINQSIDQDTALIVVEELGHIGKPAAADDPEAFLGEGAEAVEAEALPRPPVVTV
                     MGHVDHGKTSLLDYIRRAKVVQGEAGGITQHIGAYHVKTPRGVITFLDTPGHEAFTAM
                     RARGAKATDIVILVVAADDGVMPQTIEAIAHAKAAGVPIVVAVNKIDKDTANPERIRQ
                     ELTQHEVIPDDWGGTVQFIDVSAKKGTNIDALLEAVLLEAEVLELTAPVDAPAKGIIV
                     EARLDKGRGAVATLLVQNGTLKKGDMLLAGTAFGKIRAMVDENGKSITEAGPSIPVEI
                     LGLSDVPNAGEDAMVLADEKKAREIALFRQGKYRDVRLAKQQAAKLENMFNNMGETQA
                     QSLSVIIKADVQGSYEALAGSLKKLSADEVKVNVLHSGVGGITESDVNLAIASGAFII
                     GFNVRADASSRKLAENENVEIRYYNIIYDAIDDVKAAMSGMLSPEKKEQVTGTVEIRQ
                     VISVSKVGNIAGCMVTDGVVKRDSHIRLIRNNVVIHTGELASLKRYKDDVKEVRMGFE
                     CGLMLKGYNEIMEGDQLECFDIVEVARTL"
     gene            complement(9360..10700)
                     /locus_tag="WNA25_001054"
     CDS             complement(9360..10700)
                     /locus_tag="WNA25_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase/DUF3482 domain-containing protein"
                     /protein_id="HFA9823821.1"
                     /translation="MNKQPLSLAVVGHTNTGKTSLLRTLLRDSGFGEVRNAPSTTRHV
                     EEAAISDGADMLVFLYDTPGLEDAGGVLEWLENHTDNRSDGIERLQQFLGSHGAHHDF
                     NQEAKVLRQVLQSDMAMYVIDAREPVLDKYRDELTILSWCAKPVMPVFNFTGGQLPES
                     WTTMLARRNLHVFAGFDTVAFDFEGELRLWENLATMLPERSTLDRLTAMRRREWQRLD
                     GGARREIADFLLDAAAFRQEVDENEDTATVLQTMQAEIRQLERQMQQRLFALYRFYHS
                     EIDGGDWMPQAFRQDPFDSELLKQYGIRTGTGAATGALIGLGLDIATLGGSLGLGTAI
                     GGFLGGILPNTRTISDKLAGRQTLHTDPETLTLLAARALDLLHVLQTRGHAAQSDIEL
                     HSRKAPWDAAKLPPELNKARSHWKWSSLNTHRPETSRAERREYAEKIGIRLAGK"
     gene            complement(10774..12114)
                     /locus_tag="WNA25_001055"
     CDS             complement(10774..12114)
                     /locus_tag="WNA25_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HFA9823822.1"
                     /translation="MLNPSRKLVELVRILDEGGFIFSGDPVQATEALRRVDGSTEEKI
                     IRRAEMIDRDRMLRDTLERVRAGSFWLWVVAASMMFTAGFSGTYLLMDNQGLNFFLVL
                     AGVLGMNTLMLAVWLATLFLRVKVGRFFSSPATWFRGKGPVNQAVLRLYADQWRQPSV
                     RWKIGATAHSLWLCTLLGMLVSVLLLLLVRQYTFNWESTLLSNAASVRAVEMLAWLPS
                     KLGFPVPDARAVIEGRLNGNIADARAWSGLLVGSIVCYGILPRLLAWVVCKILLKTSE
                     NGLDLEKTYYQAVIRRWQNKITDADTRRETVSAVSPKIVLNDAPKWALMLETEWQDGQ
                     WFEGRLAQEWLDKGVAANREQVAALETELKQKPAQLLIGVRAQTVPDRGVLRQIVRLS
                     EAAQGGAVVQLLAEQGLSDDLSEKLEHWRNALTECGAAWLEPDRVAQEGRLKDQ"
     gene            12257..12883
                     /locus_tag="WNA25_001056"
     CDS             12257..12883
                     /locus_tag="WNA25_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFA9823823.1"
                     /translation="MYAGERFNTYSHLSGLILAAAGLMLMLLKTIGHGDGYRIFSVSV
                     YGISLLLLYLSSSLYHGIAAGKLKSILKKTDHCMIYVLIAGSYTPFALVSLRNGPGWT
                     VFSLSWLLAAAGIAQELTIGRKSEKRLLSIAIYIVMGWMVLAVMKSLTASLPPAGLAW
                     LAAGGMLYSVGIYWFVNDEKIRHGHGIWHLFVLGGSITQFVSVYGYVI"
     gene            complement(12948..14366)
                     /locus_tag="WNA25_001057"
     CDS             complement(12948..14366)
                     /locus_tag="WNA25_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HFA9823824.1"
                     /translation="MENILSVLVGTVNRFLWGYLLIYALLGIGLFFTLYLGAPQITKL
                     GAGFKSVFGGLFAKGDKDDKSLSQFQALAVAISAQIGTGNVAGVATAITAGGPGAIFW
                     MWLSAVLGMSTIFAEALLAQKYRVVSHGKYIGGPAFYITHGLTPKIGRGAARFLSGFF
                     SIALIVALGFIGNATQANSIASAVTIAFDVPSLAVGIVFAVLAGMVVIGGVNRIANIA
                     RFVVPFMAVVYILCAVVILFEFSDHIVPMFNHIFTAAFNPEAVLGGAAGIGMREAIRF
                     GVARGLFSNEAGMGSTPHAHATADVKHPVQQGMTAFVGVFIDTILVCTATALIILLTD
                     ANLSGEQGAAVTQFAFNKAFPGFGSQLLAVCLTFFAFTTIIGWYYFGESNIRFLFRGR
                     HLGIYRALVLLAIVLGTLGKVDLVWNLSDMFNGFMVIPNLIALFLLRKEIRAIYDDYL
                     AQKKAGRDLSYQYEFHEFHDKQ"
     gene            14693..15421
                     /locus_tag="WNA25_001058"
     CDS             14693..15421
                     /locus_tag="WNA25_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFA9823825.1"
                     /translation="MAGHSKWANIQHKKARQDAKRGKIFTRLIKEITVAARMGGGDPG
                     ANPRLRLALEKAAENNMPKDNVQRAIDKGTGNLEGVEYIGLRYEGYGIGGAALMVDCL
                     TDNKTRTVADVRHAFTKNGGNLGTDGCVAFNFVHQGYLVFEPGVDEDELMEAALEAGA
                     EDVVTNDDGSIEVITAPNDWAGVKSALEAAGYKSVDGDVTMRAQNETELSGDDAVKMQ
                     KLIDALEDLDDVQDVYTSAVLNLD"
     gene            complement(15480..15968)
                     /locus_tag="WNA25_001059"
     CDS             complement(15480..15968)
                     /locus_tag="WNA25_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HFA9823826.1"
                     /translation="MTPLFRKAVWLLFAVSVCAFAGSLAAQYVLGMEPCVLCISQRLC
                     VLATALCAAVVLACKPKGRVGGLSGAVFISIPAVTGISVAAYQLWLQSLPPGTAPSCG
                     APWTFRLKGWPLFDWFEPVVRGFGNCAEPDYLLGVALPVWSAAYFLAVVLTVWWAWAR
                     AK"
     gene            complement(16990..17454)
                     /locus_tag="WNA25_001060"
     CDS             complement(16990..17454)
                     /locus_tag="WNA25_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HFA9823827.1"
                     /translation="MKELDKIDFRILKILQQNARIPMTELAEKVGLSSTPVTERVCRL
                     EREHYISGYHAHLNPHLLGKPPLVFVELKLQSKSGNIFEDFKKEVLKIPQIMECHLVS
                     GEYDYLIKVRLPDMSAYRDMLGNILLQLPAASESRSYVVMEEVKENPVLDLD"
     gene            17676..18734
                     /gene="alr"
                     /locus_tag="WNA25_001061"
     CDS             17676..18734
                     /gene="alr"
                     /locus_tag="WNA25_001061"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFA9823828.1"
                     /translation="MRPLNVQIRLGNLRHNYRILKEMHGGKLLAVVKADAYGHGAVRC
                     AFALADLADGFAVATIDEGIRLRESGITHPIVLLEGVFEASEYEAVEQYSLWPAVGNQ
                     WQLEALLSRHWKKPVKVWLKMDSGMHRTGFFPHDYASAYAALKQSEYVDSIVKFSHFS
                     CADEPESGMTEIQMEAFDLGTKGLEGEESLANSAAILNIPEARRDWGRAGLALYGISP
                     FGGSDDRLKPVMRLSTRIFGERVLQPHSPIGYGATFYTSKSTRVGLIACGYADGYPRR
                     APSNSPVAVDGKLTRVIGRISMDMMTIELDASQEGLGHEVELWGDTVNINTVAEAAGT
                     IPYELMCNIKRAKFTYIE"
     gene            complement(19276..20631)
                     /locus_tag="WNA25_001062"
     CDS             complement(19276..20631)
                     /locus_tag="WNA25_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HFA9823829.1"
                     /translation="MSIQSGEILETVKMVADQNFDVRTITIGIDLHDCISTDIDVLNQ
                     NIYNKITTVGKDLVATAKHLSAKYGVPIVNQRISVTPIAQIAAATKADSYVSVAQTLD
                     KAAKAIGVSFIGGFSALVQKGMSPSDEVLIRSVPEAMKTTDIVCSSINIGSTRAGINM
                     DAVKLAGETIKRTAEITPEGFGCAKIVVFCNAVEDNPFMAGAFHGSGEADAVINVGVS
                     GPGVVKAALENSDAVSLTEVAEVVKKTAFKITRVGELIGREASKMLNIPFGILDLSLA
                     PTPAVGDSVARILEEMGLSVCGTHGTTAALALLNDAVKKGGMMASSAVGGLSGAFIPV
                     SEDEGMIAAAEAGVLTLDKLEAMTAVCSVGLDMIAVPGDTPAHTISGIIADEAAIGMI
                     NSKTTAVRIIPVTGKTVGDSVEFGGLLGYAPVMPAKEGSCEVFVNRGGRIPAPVQSMK
                     N"
     gene            complement(20646..20918)
                     /locus_tag="WNA25_001063"
     CDS             complement(20646..20918)
                     /locus_tag="WNA25_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HFA9823830.1"
                     /translation="MNNSVITVIGKDRVGIVYDVSKILAENRINILNISQQLMDDFFT
                     MIILVDTSKCPKSRQEILDLFAEESKKLALDIRMQNEEIFQAMHRI"
     gene            complement(21043..21780)
                     /locus_tag="WNA25_001064"
     CDS             complement(21043..21780)
                     /locus_tag="WNA25_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HFA9823831.1"
                     /translation="MKKLSRIVFSIVLLGFSAALPAQTYSVYFNQNGKLTATMSSAAY
                     IRQYSVAAGIAHAQDFYYPSMKKYSEPYIVASTQIKSFVPTLQNGMLILWHFNGQKKM
                     AGGFSKGKPDGEWVNWYPNGKKSAVMPYKNGLSEGTGYRYYRNGGKESEIQFKQNKAN
                     GVWKQWYADGSIKTEMVMVNDEPAKILTWDESGRLLSELSIRHHKRNGVVLEWYEDGS
                     KKSEAVYQDDKLVRKTQWDKDGYLIEP"
     gene            complement(21781..21906)
                     /locus_tag="WNA25_001065"
                     /pseudo
     CDS             complement(21781..21906)
                     /locus_tag="WNA25_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212671.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
     gene            complement(21918..22817)
                     /gene="prmB"
                     /locus_tag="WNA25_001066"
     CDS             complement(21918..22817)
                     /gene="prmB"
                     /locus_tag="WNA25_001066"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFA9823832.1"
                     /translation="MFNQAAQELTTIRDVLRFAVSRFNEAGLFFGHGTDNAHDEAVYL
                     ILHTLNLPLDMLAPYLDAKLLEAEKEEVLAVIERRAVEHIPAAYLTHQAWQGEFDFYV
                     DERVIVPRSFIYELLGDGLRPWIEYDELVHNALDLCTGSGCLAIQMAHHYPDAQIDAV
                     DVSLDALEVAGINIEDYGLEERIQLIHTDLFEGLEGTYDLIVSNPPYVDAESVGALPE
                     EYLHEPELALGSGADGLDATRQILLNAAKFLNPKGVLLVEIGHNRDVLEAAYPELPFT
                     WLETSGGDGFVFLLTREQLLGEE"
     gene            23023..23634
                     /locus_tag="WNA25_001067"
     CDS             23023..23634
                     /locus_tag="WNA25_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823833.1"
                     /translation="MDNRKRRQGVRCLQCQRRDGRNRFARAFLPRCVDDTRASKWQVR
                     GCSRCRADRQAGERQPRDGNGRWTAGEVVSVSGGELGEFAETKELRKAAMQYARDNFV
                     GKSYVNESSGHELKVTWQGVKHAASKSNSIELSLMVALDKVLMNAKYEGSFPNKLGRD
                     HIIAVHKYSTGVRFGNKVLKIRIIAREKFDGIKHYDHFILKDK"
BASE COUNT         6260 a         6471 c         5849 g         5145 t
ORIGIN      
        1 tggaaaacac ccgcttcaaa acccacgaag cctcgttggg cgtgcgctac cgcttctgat
       61 tcgccgatac cgatgccgtc tgaaccttca gacggcatga ggtctttgcc tgcgcacttg
      121 gtgcgctggt cgcctccgaa catggcgcaa cacccgacat ttccgccgaa cgcatcgggc
      181 gtttcataaa cccggtttaa aatgcatgga aaaatgccgt ctgaaagcct ttcagacggc
      241 attttgtttg agattccgtt taccaatggc tgacaaacgc ttccaaatcg gtattcttgg
      301 gcttatgcac ttcctctgtc ggcgtgccga ccatcatcag cccgatgatt ttatccttat
      361 cctcgcaacc gaaagcctcc cgcaacaatg ggctgttgac ccacatcccc gtaatccaga
      421 cattgtcgaa tccctgagcc gttgccgcca gttgcagcgc atacgccgca caacccgccg
      481 tcagcatctg ctcccattcc ggtttcggct taggcacatc gcggttcggc acaaacgtta
      541 ccccgatgac catcggcgcc atattgccca ctttttccgc ctttttcatc gcatcgtcgc
      601 cgaaattcag ttcggcaacc gtttgcttca acacatcgcg aaaacgttgc aatcctacct
      661 cgccttgaat cacggtaaaa cggaaggggc gcatgttgcc gtgatcggaa acttgggttg
      721 ccgcctgcaa tatttgttcc aactccgccg catcgggggc ggggtgcttc agctttttgg
      781 aagatcggcg gttcgtcaat aattttaaag catccatatc gttatactcc ggtcatcggt
      841 cgggtgtttc ggacacatat gccgtgaagg cttcagacgg catatccggc atcagcgcgg
      901 acggcggcag gctgccaata tatccatttc cttccgatag gtttggctgt tggaaatgtc
      961 catcagcccc aataccgtgc tgaaatagtg gtcgtgcgaa tattcgtttt ccgccgcttt
     1021 ttgtttgagg cattggaaat ctatgccccc gtgttggcgg aaggctttgg aaaaccacat
     1081 aaccatcggg atatgcgtct gtccggaagg cgcgatggcg taaggcgcgg cgtgcaggta
     1141 catcccgttt tcgcccaaac tttcgccgtg gtcggaaaca taatacaccg cgctttccaa
     1201 atcgtcgcgg ttttcaagtt tgcggataac cttgtcgata aactgatcca catacaaaac
     1261 cgtattgtcg taagtgttga ccaacgtggc gcgggcgcat ttgtcgattt cgttggtatc
     1321 gcatgtcggc gtgaatttgc gctcggcttc ggtgtagcgt tcgtaatacg tcggcccgtg
     1381 gctgccgatg gtatgcagga ttaaaaccgc gtctttatcg tttttgttga gggcttcgtc
     1441 gaacttggtc aacaggatat tgtcgaggca ctcgccgttg cggcagtatt cgggcaggtt
     1501 gagcgaagta acgtcggtat tcggcacttt gccgcacacg cccttgcagc cggaatcgtt
     1561 ttccaaccaa gtaacttcca cgccggcgcg ctgcacgatg tccagcaggt tgtcttggtg
     1621 ttcggctttg atttcgtcat aatccgtgcg gtcgaaggtt gagaacatac acggcaggga
     1681 gtgcgcggtc gatgtgccgc agcttctgac ctgcgggaaa ttgacaattt catcgccgcg
     1741 cgcggcaagc agcggcgtag tttggcggct gtaaccgttc aaaccccagt tggcggcacg
     1801 cgtagtctcg cccacgacca gcaccacgaa acggcgcagg ctgccggccg gccggttttg
     1861 cacgaccgcc atatccaatt gcgtataagg aatattggaa cgcttccaat ctttgtattt
     1921 cgacacgccc gcgccgatga aattagacgg cacaatcaga tgggttactg atttattgtt
     1981 gcggaaaaac gaggcgtaat cctgatattg caacatcgcg atgcccaacg cgcacaaaaa
     2041 ggaaacggcg gcaagcacaa ggcgcgtcaa aagctcctta taccaaacgc ggtatttgac
     2101 cttgacggcg atatatgcca gcgcgggcaa tacgcccaaa catacaatcc acagcacata
     2161 gcccggcgta atcaggcgcg cgctttccgc cgccgtggtt tgcaagacat tattcaacat
     2221 cgacttgttg aaatagatat tgaaaaatat ttcttggtaa gacaccgccg cactgataac
     2281 caatatcaat ggaatcaaca ctttatgcac aaaaggcagg gcaatgacgt gaaaaacgaa
     2341 attacttaaa aaaaacagca ccaccggcat cgtatagagg aagatgtccg ccccggtgtc
     2401 gttaaaaggg cgaagttcga caactttggc aaaaaaggcg taattcaata ccagcgaaga
     2461 atacagggaa agaaaggcaa tcagcgcgga agagccgagc ttcggcctca ggttcggttt
     2521 tatcatttgg aatgtgtcgg ataagggttg gaaaaggcat ccggaatccg gattattgaa
     2581 aaagattctt aattataagg caacggagca aagcagggca agaaaacgag taggcggcgc
     2641 gggggtttcc gcccgccatt caaacgtccg gcagacataa aaacatcgta agcaaaattc
     2701 gaaccggtct gcaaccgccc ctgccggaaa agcgggcaaa gcatttcata ttggaaaaac
     2761 ccggccgcgc cgccgacggg acagtccggc acaaacagca tcacgcccgg attgaaaagg
     2821 acggatagcc gccggcagca caatcttacc acctccaaaa cgccgccgga gaacgccgaa
     2881 acagccgatg ccgtctgaaa cgcattcggg cggcatttga aaatcaaccg taacgccgtt
     2941 ggaaatcgcg cataaaatct gccaaagccc gcacgccttc aagcggcatc gcattataaa
     3001 tgctggcgcg catcccgccg acagacttat agcccttcaa caggcacaag ccctgcaatt
     3061 cggcttccag cacaaaacgg cggtccaagt cttcgcttgc ggttttgaag accacgttca
     3121 ttttggagcg cgcatcagga tgaatatcgt tgatgtaaaa accgccgctg ccatctatcg
     3181 tctcatacaa ggtttgcgcc ttcagccgat tgaccgcttc aatttttttc acaccgccct
     3241 gcgcctgcag ccagcggaac accagccccg acatataaat cgcgtaagtt gacggcgtgt
     3301 tgtacatacc gtcgcggttc aaatgagaac ggtagttgaa cacatcggga atatcgttcg
     3361 gacaacgctc gagcaaatcc tcacgcacaa tcaccaccgt aactcctgcc ggcccgatgt
     3421 ttttctgtgc gcctgcgtaa atcagtccgt agtcggcaac atcaaactca cgcgacagaa
     3481 tctcgctgga catatcgcac accaacggcg gcatgccgtc tgaaaggcgc ggcacttcac
     3541 ggtattgcag cccgttgaca gtttcattga cggcaaaatg gacaaacgcc gaatcgggtg
     3601 caacatccca cgtttccaca ggcggcaggt caagatagtc gaactgctcg cccccgtgcg
     3661 ccgccaaacg gatttccgta tcggtcaaac ggctcatctg ttcataggcg atacggctcc
     3721 agttgcccgt taccaccgcg tcggcagtgc ggaaaccgtg tgccagattc atggctgcca
     3781 tattaaattg ggttgttgct ccgccctgaa gaaacaatat cttatagttg tcaggcactt
     3841 tcaaaagctg cctcaaatcc tgttccgcat gatgcaggat gctcaaaaac atttccgaac
     3901 ggtggctcat ttccatcacg gaaaaacccg taccgttgta gtccgacatt tcctgctgcg
     3961 ccgtcctcaa cacagcttca ggcaacacgg cagggccggc ggaaaaattg taaatcggat
     4021 aaagagacat gatgcagccc tggttctgaa caataacccg cccgatttta ggcttgcagc
     4081 aggctttgtg caaggatggg aaatacctgc cctccgcccg attccatgcc gcccgaacac
     4141 gggaaataat atcaatatat tgatttacaa acataaaaat catgctagcg acaaatagat
     4201 acatttgttt tgtcaacaat attcacgatt tcccattaca aacctcccgt acacccgctt
     4261 ttttccgtcc caaaaacaca aaataaatca acactttcat ttctccgcaa aagcggttat
     4321 aatcacgccg atttttcaac ttttgacgaa aaatggccac ggggccattt ttttattatg
     4381 tacataggga gcagcatgga tattcaaacc atcctcgaaa aaaccctgcc cggcctggga
     4441 tacgaactgg tcgatttcga actggccgcg caaggaacat tgcgcgtgtt catcgacaaa
     4501 gaaggcggca ttaccgtcga agactgcgcc accgtcagca accacttgag ccgcgtcttc
     4561 atggttgaag acatcggcta caaaaacctg gaaatttcca gccccggact cgaccgcccc
     4621 ttgaaaaaag ccgccgactt cgtgcgcttt gccggtcaga atgccaaaat caaaacccgc
     4681 ctgccgatag gcggtcagaa aaactttatc ggtaaaatcg aaggctgcga aaacgacact
     4741 gttaccgttt ccttcgacgg caaaaccgtg caaatcgaat tgggcaacat cgacaaagcc
     4801 cgcctgcgcc ccgaattcaa attctaaaac acagcaatat tggagatgtt caaaatgagt
     4861 cgtgaaatgt tacagctggc agaagcactg gcaagcgaaa aaaacgttga tgcggaagtc
     4921 gtcttccaag cactggaatt cgcactctct accgccgcca agaaaaaggc agaccgcgaa
     4981 cacatggacg tgcgcgtcca aatcaaccgc gataccggcg aataccaaac cttccgccgc
     5041 tggctgattg tggccgatga agactatacc tatcccgatg tcgaaaaaac catcgaggaa
     5101 atccaagagg aaatccccga tacgaccatc caaatcggcg aatactacga agagcagctg
     5161 cccaacgaag gcttcggccg ccaagcggcg caaactgcca aacaaatcat cctgcaacgc
     5221 atccgcgatg ccgagcgcga gcagaatctg aacgagttcc tcgccgtcaa agaagacatc
     5281 gtgtccggca cggtcaaacg cgtcgaacgc cacggcatca tcgtcgaagt cgttgccggc
     5341 aaactggacg cgctgattcc gcgcgaccaa atgattccgc gcgaaaactt ccgcagcggc
     5401 gaccgcatcc gcgccctctt cctgcgcgtc gaagaaatcg gcaacaccgg ccgcaaacaa
     5461 gtcatcctga gccgtacttc cggcgatttc ctcgtcaaac tgtacgccaa tgaagtacct
     5521 gaaattgcag gcggcatgct tgaaatccgc gctgtcgccc gcgacccggg ccaacgtgcc
     5581 aaagtcgccg tcaaagccaa cgaccagcgc atcgatccgc aaggcacctg tatcggcgtt
     5641 cgcggttcgc gtgtcaatgc cgtcagcaat gaattgtccg gcgagcgcat cgacgttgtc
     5701 ctttggtcgc ccgagcctgc ccaattcgtc atgagcgcgc tctcacccgc cgaagtcagc
     5761 cgcatcgtca tcgacgaaga caaacacgcc gtcgatgtca tcgttgccga ggaccggctc
     5821 gcgctcgcca tcggacgcgg cggtcaaaac gtgcgccttg cttccgacct gaccggctgg
     5881 cagctcaaca tcatgacttc cgccgaagca gacgaacgca atgcggcaga agatgccgcc
     5941 atccgccgcc tgtttatgaa tcacttgaat gtagatgaag aaaccgccga cgtactggtt
     6001 caagaaggtt ttgcaacctt ggaagaagtc gcctatgttc ctgccgccga actgcttgcc
     6061 attgaaggat ttgacgaaga aatcgtcgat atgctccgca accgtgcccg cgatgccatc
     6121 ctgaccatgg cgattgctgc cgaagaaaaa ctgggcgaag tgtccgacga tatgcgtaac
     6181 ctcgaaggcg tagatgccga tatgctcctc agccttgccg aagcaggcat taccacccgc
     6241 gacgacttgg cagagcttgc cgtggacgaa ctgattgaaa tcaccggtgt aaacgaagaa
     6301 actgcaaaag ccgtcatcct gaccgcacgc gaacactggt ttaccgaaga caaataaagg
     6361 gggtacagat gagtaacaca accgtagaac aatttgccgc cgagctgaaa cgccccgtcg
     6421 aagacctgtt gaaacagttg aaagaagccg gcgtcagcaa aaacagcggc agcgattccc
     6481 tgacgctgga cgacaaacag cttctgaacg cctacctgac caagaaaaac ggcagcaacg
     6541 gcggcaccat cagcatccgc cgcaccaaaa ccgaagtcag caccgttgac ggcgtaaaag
     6601 tcgaaacacg caaacgcgga cgcactgtca acattccttc tgccgaagaa ttggcagcac
     6661 aagtaaaagc cgcccaaacc caagccgcac ctgttcagcc ggagcagacg gcagaagacg
     6721 cggtaaaagc ccgagccgaa gctgccgcac gcgcagaagc ccgtgccaag gcagaagcgg
     6781 aagcggcaaa actgaaagcg gcaaaagcag gcaacaaagc caaacctgcc gcgcagaaac
     6841 ctaccgaagc aaaagccgaa accgcacccg ttgcggcgga aaccaaaccc gccgagccca
     6901 aagaaaaagc cgtcaagccg aaacacgagc gaaacggcaa aggcaaagat gccaaaaaac
     6961 cggcgaaacc tgccgcacct gccgtgccgc aacccgtggt cagcgcggaa gaacaggcgc
     7021 aacgcgacga agaggcgcgc cgcgccgccg cattgcgcgc ccaccaggaa gccctgttga
     7081 aagagaaaca ggaacgccag gcacgtcgcg aagccatgaa acaacaggca gaacaacagg
     7141 caaaagccgc acaggaagcc aaaaccggca gacagcgtcc cgccaaacct gccgagaaac
     7201 cgcaggcagc cgcaccggcc gtcgaaaaca aacctgtcaa tccggcaaaa gcgaaaaaag
     7261 aagaccgccg caaccgcgat gacgaaggtc aaggccgcaa cgccaaaggc aaaggtgcaa
     7321 aaggcgggcg tgaccgcaac aatgcccgca acggcggcga cgagcgcgta cgcggcggca
     7381 agaaaggcaa aaaactcaaa ctcgagccga accaacacgc cttccaagca ccgaccgaac
     7441 ccatcgtaca cgaggttttg gttcccgaaa ccattaccgt tgccgatttg gcgcacaaaa
     7501 tggcggtcaa aggcgtggaa gtggtcaaag ccctgatgaa gatgggcatg atggttacca
     7561 tcaaccaatc catcgaccaa gacaccgccc tgattgtcgt cgaagaactc ggacacatcg
     7621 gcaaacccgc cgccgccgac gatcccgaag ccttcttggg cgagggcgcg gaagcggtgg
     7681 aagccgaagc attgccgcgt ccgcccgtcg ttaccgtcat gggccacgtc gaccacggca
     7741 aaacctcgct gctggactac atccgccgcg ccaaagtggt acagggcgaa gcgggcggca
     7801 ttacgcagca catcggcgcg taccacgtca aaaccccgcg cggcgtgatt accttcttgg
     7861 acaccccggg ccacgaagcc tttaccgcta tgcgcgcgcg cggcgcgaaa gcaaccgata
     7921 tcgtgattct tgtggtcgcc gccgacgacg gcgtgatgcc gcaaaccatc gaggcaattg
     7981 cccacgctaa agcggcaggc gttccgattg tggttgccgt caataaaatc gataaagaca
     8041 ctgccaaccc cgaacgcatc cgtcaggaac tgacccaaca cgaagtcatc cccgacgatt
     8101 ggggcggcac ggttcaattc atcgacgttt ccgcgaaaaa aggaacgaac atcgacgcgc
     8161 tgctcgaagc cgtattgctc gaagccgaag tattggaact gaccgcacct gtcgatgcac
     8221 ccgccaaagg catcatcgtc gaggcgcgtt tggacaaagg gcgcggcgcg gttgccacat
     8281 tgctggttca aaacggtacg ctgaaaaaag gcgacatgct gctggccggt acggcattcg
     8341 gcaaaatccg cgcgatggtc gatgaaaacg gcaaatccat taccgaagcc ggcccgtcca
     8401 tccccgtcga aatcctcggc ttgtccgacg taccgaatgc gggtgaagac gcgatggtat
     8461 tggcggacga gaaaaaagcg cgcgaaatcg ccctcttccg ccaaggcaaa taccgcgacg
     8521 tgcgccttgc caaacagcag gcggcgaagc tggaaaatat gttcaacaat atgggcgaaa
     8581 cccaggccca atctttgtcg gtcatcatca aggcagacgt tcagggctct tacgaggctt
     8641 tggcgggcag cctgaaaaaa ctgtccgccg acgaggtgaa agtgaacgtg ttgcacagcg
     8701 gcgtgggcgg cattaccgaa tcggatgtca accttgccat cgcttcgggc gcgttcatta
     8761 tcggctttaa cgtgcgtgca gatgcctctt cgcgcaaact tgccgaaaat gaaaacgtgg
     8821 aaatccgcta ctacaacatc atctacgatg ccatcgacga cgtgaaggcg gcgatgagcg
     8881 gcatgctttc tcccgaaaag aaagaacagg ttaccggtac ggtcgaaatc cgtcaggtca
     8941 tctccgtttc caaggtcggc aacattgcag gctgtatggt caccgacggc gtggtcaaac
     9001 gcgattccca tatccgcctc atccgcaaca acgtggtcat ccacacgggc gaactggctt
     9061 cgttgaaacg ctataaagac gacgtaaaag aagtccgcat gggcttcgag tgcggtctga
     9121 tgctcaaagg ctacaacgaa atcatggaag gcgaccaact ggaatgcttc gacatcgtcg
     9181 aagttgcccg caccctgtaa ttcctttgca aataaaatgc cgtctgaagc gttcagacgg
     9241 caacgaaacg ggttctgtat catacagaac ccgttttttg tcgcaaatcg gcttcagcga
     9301 ccctcctgcc ttatcccgat ttgaatctga cttgccatac aaacaggctt cagacggcat
     9361 tatttgcccg ccaaacgtat cccgatcttc tccgcatatt ccctgcgttc ggcgcggctg
     9421 gtttccgggc ggtgcgtatt gagcgacgac catttccaat gactgcgggc tttgttgagt
     9481 tcgggcggga gtttggcggc atcccacggg gctttgcggc tgtgcagctc gatatccgac
     9541 tgtgccgcgt gtccgcgcgt ttgcaggacg tggagcagat cgagggcgcg ggcggcgagc
     9601 agggtcaggg tttcagggtc ggtgtgcagg gtttgacggc cggcgagttt gtcggaaatg
     9661 gtgcgggtat tgggcaggat gccgcccaaa aagccgccga ttgccgtacc caatccgagc
     9721 gagccgccga gtgtggcgat gtccagcccc aagccgatga gcgcgccggt tgccgcgccc
     9781 gtgccggtgc ggatgccgta ttgtttgagc aattcgctgt cgaacgggtc ttggcggaag
     9841 gcttgcggca tccagtcgcc gccgtcgatt tcgctgtggt agaaacggta aagggcaaac
     9901 agccgctgct gcatctgccg ttcgagttgg cgtatttccg cctgcatggt ttgcagcacg
     9961 gtggcggtat cctcgttttc gtccacttcc tgcctgaagg cggcggcatc gagcaggaaa
    10021 tcggcgattt cgcggcgcgc tccgccgtcc agccgctgcc attcgcgccg gcgcatggct
    10081 gtcaggcggt caagtgtgct gcgttcgggc aacatggtgg cgaggttttc ccacaggcgc
    10141 aattcgcctt caaaatcaaa ggcgacggtg tcgaaccctg cgaaaacgtg caggtttctc
    10201 ctcgccagca tggttgtcca cgattcggga agctgtccgc cggtaaagtt gaacacgggc
    10261 ataaccggtt tggcacacca tgaaaggatg gtcaattcat cccgatattt gtcgaggacg
    10321 ggttcgcgcg cgtcgatgac gtacattgcc atatcgcttt gcaaaacctg ccgcagcact
    10381 ttggcttctt ggttgaaatc gtgatgcgcg ccgtggctgc cgagaaactg ttgcagccgc
    10441 tcgatgccgt ctgaacgatt gtccgtatgg ttttccagcc attccaaaac accgcccgcg
    10501 tcttcgagtc cgggcgtgtc atacaggaaa accaacatgt ccgcgccgtc gctgatggcg
    10561 gcttcttcga catggcgtgt ggtggacggt gcgtttctga cttcgccgaa gccgctgtcg
    10621 cgcaacaggg tgcgcaggag cgaggttttg ccggtgttgg tgtgtcccac gacggcgagg
    10681 gaaaggggtt gtttgttcat gatgtttttg aagaatggat tttcagacgg tcttttttca
    10741 gaatggcggc ttaacagaac atttcaagtg agtttattgg tctttcaaac ggccttcctg
    10801 cgccaccctg tcaggctcaa gccacgccgc gccgcattcg gtcagcgcgt tacgccaatg
    10861 ttccagcttt tccgaaaggt cgtctgaaag cccctgttcc gccaaaagct gcaccaccgc
    10921 gccgccctgc gccgcttccg aaagccgcac aatctgccgc agcacgcccc ggtccggcac
    10981 agtttgggcg cgtacgccga taagcagttg cgccggtttc tgcttcagct ctgtctccag
    11041 cgcggcaacc tgttcccgat tggcggcaac gcccttatcc agccattcct gcgccagcct
    11101 gccctcgaac cattggccgt cctgccactc ggtctccagc atgagcgccc atttcggcgc
    11161 atcgttcaag acgattttcg gcgaaacggc ggacacggtt tcccgacgcg tatccgcatc
    11221 ggtgattttg ttctgccagc ggcggatgac cgcctgataa taggtttttt ccaaatccaa
    11281 tccgttttcg cttgttttca aaaggatttt acacactacc caagccaaga ggcgcggcag
    11341 gatgccgtag cagacgatac tgccgaccag cagccccgac caagcccgcg catcggcaat
    11401 attgccgttc agacgacctt cgatgaccgc ccgcgcatcg gggacaggga aaccgagttt
    11461 cgacggcagc catgccaaca tttccaccgc gcgtaccgaa gcggcattgc tcaacagcgt
    11521 gctttcccag ttgaacgtat attgccgcac caaaagcagc agcaataccg acaccagcat
    11581 tccgagcagc gtgcagagcc acaagctgtg cgccgttgcg cctattttcc atcgtaccga
    11641 aggttgccgc cactggtccg catacagccg caacaccgcc tgatttacag ggcctttgcc
    11701 ccgaaaccac gtcgccggac tgctgaaaaa ccgccccact ttcacgcgca ggaacaacgt
    11761 tgccaaccat actgccagca tcagcgtatt catgcccaac actcccgcca aaactaaaaa
    11821 gaaattcagc ccctgattgt ccatcagaag ataagtgcct gaaaatccgg cggtaaacat
    11881 catcgatgcc gccaccaccc ataaccagaa cgaccccgca cgcacacgtt ccaacgtgtc
    11941 ccgcagcata cggtccctgt cgatcatctc cgcccgacgg atgatttttt cctccgtact
    12001 gccgtccacg cggcgcaaag cctccgtcgc ctgcacggga tcgccgctga aaataaaacc
    12061 gccttcatcc aaaatacgga ccagctcaac cagttttcgg gatggattca acataaaatg
    12121 ccgtctgaaa ataaaaaaca aattttaaca cacgcatttt caagaatatt cacagtgtag
    12181 gcaaagagta aatcccacac agaggcaaaa gtatcggcgt aaactgactg cctctacttt
    12241 cccgaaagat tgtgcgatgt atgcaggcga acgcttcaat acttacagcc atttgagcgg
    12301 tttgattctg gcggcggcag gtttgatgct gatgctgctg aaaaccatag gacacgggga
    12361 cggctaccgt atcttcagcg tatcggttta cggcatcagc cttcttctgc tctatttgag
    12421 ttcctcgctg taccacggaa ttgcagccgg aaaactgaaa agcattttga aaaaaaccga
    12481 ccactgcatg atttatgtgc tgattgccgg aagctacaca ccgtttgcac tggtttcttt
    12541 gagaaacggg ccgggctgga cggtattttc actgtcctgg ctgctggcgg ctgcaggaat
    12601 cgcacaagaa ctcaccatcg gacggaaaag cgaaaaacgt ctgctgtcta ttgcgattta
    12661 tatcgtaatg ggctggatgg tcttggcggt aatgaaatcc ctgacagcct cactcccgcc
    12721 ggcaggactg gcttggctgg cggcaggcgg tatgctgtac agcgtcggca tttactggtt
    12781 tgtaaacgat gaaaaaatcc gacacgggca cggaatctgg catctgttcg tattgggcgg
    12841 cagcataacc caatttgtca gcgtgtacgg ttatgtaatc tgaatgccgt ctgaaacaca
    12901 aaaacctccc aaacctgaag gcagggaggt tttctctttg ccgggcatta ttgtttatcg
    12961 tggaactcgt ggaattcata ctgataggac aaatcccgac ccgctttttt ctgtgccaaa
    13021 taatcatcat aaatggcgcg gatttcctta cgcagcaaga acagggcaat caggttgggg
    13081 ataaccataa agccgttgaa catatcggac agattccaaa ccaaatcgac tttgccgagc
    13141 gtacccaaaa cgatagcaag cagaaccaat gcgcgataga tgcccaagtg tcttcccctg
    13201 aaaagaaaac ggatgttgga ctcgccgaaa taataccagc cgataatggt cgtaaaggcg
    13261 aagaaggtca gacacacggc aagcaattgc gaaccgaagc ccggaaatgc cttgttaaag
    13321 gcaaattgag taaccgccgc gccctgttcg cccgaaaggt tggcatcggt cagcaggata
    13381 atcaatgccg tagccgtaca taccaaaatc gtatcgataa atacaccgac aaatgccgtc
    13441 ataccttgct gaacaggatg cttcacatcc gcagtcgcgt gggcgtgcgg agtcgaaccc
    13501 atacctgctt cgttggaaaa cagaccgcgc gccacgccga aacgtatcgc ttcacgcata
    13561 ccgatacccg cagcaccgcc caaaacggcc tcgggattga aggcggcggt aaagatgtgg
    13621 ttgaacatcg gcacaatatg gtcggaaaat tcaaacagga taacgacggc gcacaaaata
    13681 taaacaaccg ccataaacgg cacgacaaac cgggcaatat tggcaatacg gttcacgccg
    13741 ccaatcacaa ccatgcccgc aaggacggca aacacaatac cgactgccaa agaaggcaca
    13801 tcaaatgcaa tggtaacggc agaagcgatg gaatttgcct gagtcgcatt gccgataaag
    13861 cccaacgcga caatcagcgc gatggagaaa aagcccgaca ggaaacgcgc cgcgcccctg
    13921 ccgattttcg gagtcagacc gtgcgtaata taaaacgccg gcccgccgat gtatttgccg
    13981 tggctgacga cgcggtattt ctgcgccagc agtgcttccg caaaaatcgt ggacatcccc
    14041 aaaacggcag aaagccacat ccaaaaaatc gcacccggcc cgcctgcggt aatggcggtc
    14101 gccacaccgg cgacgttgcc cgtaccgatt tgcgcggata tagcaaccgc caacgcctga
    14161 aactgcgata aggacttatc gtctttatca cctttggcaa acaagccgcc gaatacggat
    14221 ttgaatcccg cgcccagctt ggtaatctgc ggcgcaccaa gatacagcgt aaaaaacagg
    14281 ccgataccca aaagcgcata aatcagcagg tagccccaaa gaaaccgatt gactgtaccc
    14341 accagaacag acaatatatt ttccataaaa taaaccttat cttacgatta aaatgactgt
    14401 tttccaaaag acattccaat aaggaaacac ggcgagcaga ccgtatttgc cgcaacagat
    14461 gccttaaatt gtcaataatc cgagagaagc tgtgccagca taccgtaaaa tatcgttaat
    14521 taaaatattt ctttattttt aagcggaaag cggaggaaat cgttttcaga cggcatcgac
    14581 aacggcacgg cataaaacag gatattttgg gtacttgcaa cttatgttaa aatgccgacc
    14641 gtaaaaaatc tgacaaaaac agattaatta tttgaaataa gaaaggaaat ttatggcagg
    14701 ccatagcaag tgggcgaata tccagcataa aaaagcccgt caggatgcca aacgcggcaa
    14761 aatcttcacc cgtttaatca aagaaatcac cgttgcggcg cgtatgggcg gcggcgatcc
    14821 cggcgcaaat ccgcgcctgc gtctggcttt ggaaaaagca gccgaaaaca atatgcccaa
    14881 agacaatgtg caacgcgcca tcgacaaagg tacgggtaac ttggaaggcg tggaatacat
    14941 cgggttgcgc tacgaaggct acggcatcgg cggcgcagct ttgatggtgg actgcctgac
    15001 cgacaacaaa acccgcaccg ttgcggacgt acgccacgca tttaccaaaa acggcggcaa
    15061 cttgggtacc gacggctgcg tggcgttcaa cttcgtgcat cagggctatt tggtattcga
    15121 acccggcgtt gacgaagacg agctgatgga agcggctttg gaagccggtg cggaagacgt
    15181 ggttaccaac gacgacggtt ccatcgaagt cattaccgcg ccaaatgatt gggcgggcgt
    15241 aaaatccgct ttggaggcgg caggttacaa atccgttgac ggcgacgtta cgatgcgcgc
    15301 ccaaaacgaa accgaactct ccggcgacga tgccgtcaaa atgcaaaaac tgattgacgc
    15361 gctggaagac ttggacgacg tgcaagacgt ttacacttcc gccgtattga atctggactg
    15421 atacgcagca cagcagacat acaacaaaat gccgtctgaa cctttcagac ggcattgatt
    15481 tatttagccc ttgcccacgc ccaccaaacc gtcaggacaa ccgccaaaaa atacgccgcg
    15541 ctccaaacag gcaaagcaac ccctaacaga taatccggtt cggcacaatt tccgaacccg
    15601 cgcacaacag gctcgaacca atcaaacaaa ggccagccct tcaatcgaaa cgtccacggc
    15661 gcgccgcacg aaggagccgt acccggcggc agcgactgca accacaactg atatgccgca
    15721 acagaaatac ccgtaacggc gggaatactg ataaacactg cgcctgacag tccgcccacc
    15781 ctgcccttgg gcttacacgc cagcacaact gccgcacaca atgcggttgc caaaacacat
    15841 aaccgctgac tgatgcacaa aacgcaaggc tccataccca aaacatactg tgccgccaaa
    15901 gaaccggcaa atgcacagac cgaaacggca aacagcagcc aaacggcttt tctaaataac
    15961 ggggtcattt tctcaacaca ccaatcaaaa taccgatatg ccgattttgc tggatatatc
    16021 ccgagatggc aagggacgaa tcgacaccgg cacaggtgcg gacgatttcg gttttcccga
    16081 tatgcacaaa acctgacaaa ggacaaaacg gcgatttgtc cgcacaatgc ccgaaaaaat
    16141 acatccggag gatttgaatt tgaatttcgg caaatttagc gaatcaaaag caacagccga
    16201 taaaaaaatc aaaagcttat ttcaatgttt caacacacag gacgacacat aaagcaccgc
    16261 cctatgtgcc gtcctgattt ggaaggggtt acacccctcc caaatatagt ggattaacaa
    16321 aaaccggtac ggcgttgcct cgccttgccg tactatttgt actgtctgcg gcttcgccgc
    16381 cttgtcctga tttttgttaa tccactataa agtctgattc taccgcccta aagggcaggg
    16441 tttcaaccga aaaggaaaca cgatgaaagc atatgatttt ttgaataatc ggattgacgg
    16501 gtttttgagg gaatttgtta ccggatagcc atcgggcaag ttttttgcaa aatttgaatc
    16561 ggtcgggtaa attttcaaaa aataattgac agcagataag aaacggcgga taattaccgc
    16621 cgttgagttg cttgatgcag cttgattttt ctcctctact tctcctttgt agacttggca
    16681 cacattcaac cggatgtgtg cattttttat ctccgctttt tttgaaattt aattactttt
    16741 attgtttgaa attctattct ttaagaaatt taacaagaaa aacgcttgct ttttgaccgg
    16801 aaaagctgca taattcgttc cgttagctgg ttcgagtagt cagttaatag tttctcctct
    16861 atttctcctt tgtagacttg gcacacattc tattgaatgt gtgcattttt tatctgaagc
    16921 aacaagcctc tgtgcgtgat gttgttatgt ttcatttaga tgtcaaaccg catacccggt
    16981 ctgaaatatt caatccaaat ccaaaaccgg attttctttg acttcctcca tcacaacata
    17041 actcctactc tccgaagcgg caggcagttg caataggata ttgccgagca tatcccgata
    17101 ggcagacata tcgggcaaac gtactttaat caggtagtcg tattcgcccg acaccagatg
    17161 gcactccata atttgcggaa ttttcagcac ttcttttttg aaatcttcga aaatattgcc
    17221 cgatttggat tgcagcttca gctcgacaaa aaccaatggc ggttttccca acagatgggg
    17281 attgagatgg gcgtgatagc cggaaatata atgttcccgc tccaaacggc ataccctctc
    17341 tgtaacgggc gtgctggaca accctacctt ctcggcaagc tccgtcatcg ggatacgggc
    17401 attttgctga agaattttga ggatgcggaa atcgatttta tctagttctt tcatttggat
    17461 taccttgtat ttattattga ttttaacaaa tagagtataa atttgggaaa gcaattatat
    17521 caggaaaagc aaaccgcctt cctacctgaa aactgctgct ctggcttgaa gacacaaggt
    17581 tctttaatat tttaaaagcc ttgccgttgg attataattc cccaattgat ttcttaattt
    17641 tgctaataaa cacttgtttg gtaaggaatg aatttatgcg gcctttgaac gtgcagatca
    17701 ggttgggcaa ccttaggcac aattatcgga ttttgaagga aatgcacgga ggcaagctgt
    17761 tggcggtagt gaaggccgac gcatacggac acggtgcggt cagatgtgct ttcgcgctgg
    17821 cagacttggc agacggcttt gccgtggcga caatcgacga gggaatcagg ctgcgggaga
    17881 gcggcattac ccatccgatt gtccttttag aaggcgtatt tgaagcatca gagtacgaag
    17941 cggtcgaaca atactcgctt tggccggcag tcggaaacca atggcagctt gaggctttgc
    18001 tttcccgcca ttggaaaaaa cctgtcaagg tctggttgaa aatggattcg ggaatgcacc
    18061 gtaccggatt tttccctcat gattacgctt cggcatatgc ggcattgaaa cagtcggaat
    18121 atgtggacag tattgtcaag ttctcgcatt tctcctgtgc ggacgaacct gaaagcggta
    18181 tgacggaaat acagatggaa gcattcgatt tgggtacgaa agggctggaa ggcgaagaaa
    18241 gccttgccaa ttcggcagct attttgaata tccccgaagc acgcagggat tgggggcgcg
    18301 cgggcttggc gttatacggc atttccccgt tcggaggcag cgatgacagg ctgaaacccg
    18361 tgatgaggct ttcaacccgt attttcggcg aacgcgtttt acagccgcac tcccctatcg
    18421 gttatggcgc aacattttat accagtaaat ctacgcgcgt cggtctgatt gcctgcggtt
    18481 atgcggacgg ttatccgcgc cgcgctccaa gcaattcccc cgtcgccgtc gacggtaaat
    18541 tgacccgggt catcggcagg atctctatgg atatgatgac tatcgagctg gacgcttcgc
    18601 aagaaggttt gggacacgag gtcgaactgt ggggcgatac ggtcaatatc aataccgttg
    18661 ccgaagcggc cggaaccatc ccttacgaat tgatgtgcaa tatcaaacgt gcaaaattca
    18721 cttatatcga gtaatcgagt ccaaacgaaa atcccgtctg aagcttttca gacggcataa
    18781 atgcgttttg acaaaatcag taaaagacat tgccaaatag aaaatactga taatgcccac
    18841 aatctgaaac cccatccttc ccgcaaaaac ggaaaatcaa aatcagaaac ctaaaatttc
    18901 gtcattcccg cgcaggcggg aatccggttt tttgagtttc cgtcatttct gataaattct
    18961 cgtcgttttt catttctaga ttcccgcatg cgcgggaatg acaaaattaa agtttcagaa
    19021 tttatttgaa caacaaacta gattcccgct ttcgtgggga tgacggcgga taggttttgt
    19081 tgtttcggat aaattcctgt tgttttttgg tttccagatt cccaccttta aaagaatgac
    19141 gtaagaaagc agaaataaga acacggggat tttttaaatt tgcaatattc ttttctaaag
    19201 acattttacc caatgcccat aaacaaaaat gccgcctgaa gcctttcaga cggcattttc
    19261 ccatcaaaac cgcaatcagt ttttcatcga ttgaaccggt gcgggaatcc tgccgccccg
    19321 gttgacgaac acttcgcacg aaccttcttt tgccggcatt acaggcgcgt agcccaacag
    19381 accgccgaac tcgacgctgt cgccgacggt tttgcccgtt accggaataa tgcgcacggc
    19441 ggtggttttg ctgttgatca tgccgatggc ggcttcgtcg gcgatgatgc cggaaatggt
    19501 gtgcgcgggc gtgtcgccgg gaacggcaat catgtccaaa ccaacggagc agacggcggt
    19561 catggcttcg agtttgtcca gcgtcaacac gcctgcctcg gcggcggcaa tcataccttc
    19621 gtcttcggaa acggggataa acgcgccgct caaaccgccg accgcgctgg aagccatcat
    19681 gccgcccttt ttcacggcat cgttcagcaa tgccaaagct gctgttgtgc cgtgcgtacc
    19741 gcagacgctc aagcccattt cttcaagaat gcgcgccacc gagtcgccga cggcgggggt
    19801 cggtgccagc gacaaatcga gaatgccgaa cgggatattc agcatttttg aggcttcgcg
    19861 gccgatgagt tcgcccacgc gggtgatttt gaaagcggtt ttcttcacga cttcggcgac
    19921 ctcggtcagg ctgaccgcgt ccgaattttc cagcgcggct ttgaccacgc ctggaccgga
    19981 tacgccgaca ttaatcacag catccgcttc gcccgagccg tggaacgcac ccgccataaa
    20041 cggattgtct tccaccgcgt tgcagaacac gacgattttg gcgcagccga aaccttcggg
    20101 cgtgatttca gccgtgcgtt tgatggtttc gcctgccagc ttgaccgcat ccatattgat
    20161 accggcacgc gtgctgccga tattgatgga gctgcacacg atatcggtag ttttcatcgc
    20221 ttcgggaacg gaacggatca acacctcatc cgaaggcgac ataccttttt gcaccagcgc
    20281 ggaaaagccg ccgataaagg acacgccgat ggctttggct gccttgtcca aagtctgcgc
    20341 cacgctgaca taactgtcgg ctttggtcgc cgccgcgatt tgggcaatcg gcgtaacgga
    20401 aatgcgctga ttcacaatcg gcacgccgta tttggcggaa aggtgtttcg ccgttgccac
    20461 caagtctttg ccgaccgtgg tgattttgtt gtaaatgttt tggtttaaca cgtcgatgtc
    20521 ggtgctgatg cagtcgtgca aatcaatgcc gatggtaatg gtgcggacat caaaattctg
    20581 gtcggcaacc attttgacgg tttctaaaat ttcgccggat tggatactca tcacattcct
    20641 ccgactcaaa tgcggtgcat cgcttggaag atttcttcgt tttgcatacg gatgtcaagc
    20701 gcgagttttt tgctctcttc cgcaaacaaa tccaaaatct cctgccgcga tttagggcat
    20761 ttcgaggtat ccaccaagat aatcatagta aaaaaatcgt ccatcagctg ttggctgata
    20821 ttgaggatgt tgattcggtt ttccgccaaa attttggaaa catcgtacac gatgccgacg
    20881 cggtctttac cgatgacggt gatgactgaa ttgttcacag gcttactcct tgcagatatc
    20941 cgttaaaatc cgaaattata ccaccgtcgg attttgaaga atattgtcaa caatatatac
    21001 atacaaaatg ccgtctgaaa ctatttcaga cagcatcaag attcagggtt cgattaaata
    21061 accatcctta tcccattggg ttttcctgac caacttgtca tcctgataaa cagcctcgct
    21121 ctttttagaa ccatcttcat accactccaa aaccaccccg ttgcgtttat ggtggcggat
    21181 agacagttcc gaaagtaatc ggccgctttc atcccaagtc agaattttgg caggctcatc
    21241 gttgaccata accatttccg tcttgatact tccatcggca taccattgct tccatacgcc
    21301 gttcgcctta ttttgcttaa actggatttc gctttccttg ccgccgttac ggtaataacg
    21361 gtatcccgta ccctcactca agccattttt ataaggcata accgcagatt ttttaccgtt
    21421 cggataccag ttgacccatt ccccgtccgg cttacccttg ctgaagcccc ccgccatttt
    21481 tttctgacca ttaaaatgcc acaaaatcaa cataccgttt tgcagggtag gcacaaaaga
    21541 tttgatttgc gttgaagcaa cgatataagg ttcggaatat ttcttcatcg acggataata
    21601 aaaatcctgc gcgtgtgcga tacccgccgc cacactatat tgcctgatat aagcggcaga
    21661 agacatcgtc gccgtcagtt tcccgttctg attaaaataa acagaatagg tctgcgccgg
    21721 caaagcggcc gaaaaaccca acagtacgat tgaaaataca atccgagata atttcttcat
    21781 tatgatagcg atataaaaaa caattaactt ataaacaata tggtaaggat ttctcaacca
    21841 agcgtcaaac ccgaaacaat gtatcgtaaa aatgccgtct gaaaacaaat cgtcttcaga
    21901 cggcatttcc ctccaactca ctcttcgccc aataactgct cgcgcgtcaa gaggaaaaca
    21961 aaaccgtcgc ccccgctggt ttccaaccaa gtaaaaggca actccggata cgctgcttcc
    22021 aatacatccc tgttatgccc gatttccacc agcaatacac ctttaggatt cagaaacttt
    22081 gccgcattca gaagaatctg cctggtggca tccaacccgt ccgccccgct gcccaacgcc
    22141 aattccggtt cgtgcaaata ttcctcaggc aaagccccaa ccgactctgc atccacataa
    22201 ggcggattgg aaacaatcaa atcataagtg ccttccagtc cctcaaacaa atcggtatga
    22261 ataagctgaa tacgctcttc cagaccataa tcttcaatat tgatccctgc aacttccaaa
    22321 gcatccaaac tcacatcaac cgcatcaatt tgggcatcag gataatggtg cgccatctga
    22381 atggcaaggc aaccgcttcc ggtgcaaaga tccaaagcat tatgcaccag ctcatcgtat
    22441 tctatccaag gtcgaagtcc gtcacccagc aattcataaa taaaagagcg cggtacaatc
    22501 acgcgttcat ctacataaaa atcaaactcc ccctgccagg cctggtgtgt caaataagcg
    22561 gcaggaatgt gttcgacagc acgacgctca ataaccgcca gcacttcctc tttttcagct
    22621 tccaagagtt ttgcatcaag atatggggca agcatatcca aaggcaaatt caaagtatgc
    22681 agaatcaaat aaaccgcttc atcatgcgca ttatctgttc catgaccaaa aaagagccct
    22741 gcctcattaa aacggctgac tgcaaaacgt aaaacatcac ggatagtcgt caattcttgt
    22801 gcggcctgat taaacataat atgaaccatt ctgcgtatag atgcttttaa ttataacaga
    22861 aacaacaagc aaaccttttc atatcgccaa ataaccactc aatctaccca tacaactaca
    22921 taaatgccgc gcgaagacca ttgcccgaac ggaaacggcg atggcggacg gtatgggcaa
    22981 cctgataggc tgggaagaaa cggggcttgt tgagggcaag cagtggataa ccgcaaaaga
    23041 cgacaaggtg tccgatgtct gcaatgccaa cggcgggatg ggcgtaatcg gtttgcacga
    23101 gcctttctcc cacggtgcgt tgacgatacc cgagcatcca aatggcaggt gcgcggttgt
    23161 tcccgttgtc gggcggacag gcaagctggc gaaaggcagc cgcgcgatgg aaacgggcga
    23221 tggacggcag gtgaggttgt ttccgtatcg ggcggcgaat tgggggaatt tgctgaaaca
    23281 aaggagcttc gtaaagcggc tatgcagtat gcgcgtgata actttgtcgg caaaagctat
    23341 gtcaatgaaa gcagcgggca tgaactgaag gtaacttggc aaggtgtgaa acacgctgcg
    23401 tcaaaatcga acagcatcga actttccctt atggtcgcgc ttgataaagt tttgatgaat
    23461 gcaaaatatg aaggaagttt tcctaacaaa ttagggaggg atcatattat tgcggtacac
    23521 aaatactcaa ccggtgtcag atttggcaat aaagtcttaa aaatcaggat aatagcccgt
    23581 gagaaatttg atgggataaa acattacgat cattttattt tgaaagacaa atgaaaaaac
    23641 cctgtccagt atatctggga tagcttatgg aagcatttaa cccagcctcg gataaggctt
    23701 taaatccatc atgccattta tacgg
//
LOCUS       DAWTQS010000027        23318 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.027, whole genome shotgun sequence.
ACCESSION   DAWTQS010000027 DAWTQS010000000
VERSION     DAWTQS010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23318
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.027"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(223..1440)
                     /locus_tag="WNA25_001068"
     CDS             complement(223..1440)
                     /locus_tag="WNA25_001068"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFA9823834.1"
                     /translation="MALIVHKYGGTSVGSPERIKNVAKRVAKARAEGHDIVVVVSAMS
                     GETNRLVALAHEMQEHPDPRELDVVLATGEQVTIGLLAMALKDIGVDAKSYTGWQVSL
                     KTDTAHTKARIESIDDEKMRADLAAGKVVIVAGFQGISSEGNISTLGRGGSDTSAVAL
                     AAALKADECQIYTDVDGVYTTDPRVVPEARRMDTVTFEEMIELASLGSKVLQIRSVEF
                     AGKYKVRLRVLSSLQDGGNGTLITFEEDDNMERAAVTGIAFDKNQARINVRGVPDKPG
                     VAYQILGAVADANIEVDMIIQNVGSEGTTDFSFTVPRGDYKQTLEILSERKDSIGAAS
                     IDGDDTVCKVSAVGLGMRSHVGVAAKIFRTLAEEGINIQMISTSEIKVSVLIDEKYME
                     LATRVLHKAFDLG"
     gene            complement(1642..2370)
                     /gene="rph"
                     /locus_tag="WNA25_001069"
     CDS             complement(1642..2370)
                     /gene="rph"
                     /locus_tag="WNA25_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFA9823835.1"
                     /translation="MPDYIRTSRAADSLRDIKITPHFLPHTDGSCLIECGNTKVICTA
                     SVDENAPPFLHGKNQGWVTAEYGMLPASTALRMRREASAGKQSGRTQEIQRLIGRSLR
                     AVVDMEKLGERQILIDCDVIQADGGTRTASITGAFVALQIAVGKLVSDGILSENPILE
                     AVAAVSAGVVNGVPLLDLDYPEDSGCDSDVNIVMTASGKIIEIQGTAEGAPFSLDELG
                     KLVALAQKGIGELLRYQQNALSVA"
     gene            complement(2452..2916)
                     /locus_tag="WNA25_001070"
     CDS             complement(2452..2916)
                     /locus_tag="WNA25_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFA9823836.1"
                     /translation="MYKHLVVAVDGSETSINALKHAAELAGVNGARLTLVHVANPAEY
                     MALAPEFLQHESYEAAAVAQGNEVLDAAERTARELGVGNTVKHLLVANKGAREMAQDL
                     VDYADENGAGLLVLGTHGRTGLMHLLMGSFAETVMRQSHLPLLIIRSKAEEA"
     gene            3133..3909
                     /locus_tag="WNA25_001071"
     CDS             3133..3909
                     /locus_tag="WNA25_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HFA9823837.1"
                     /translation="MSDNALTSSRRFGGIARLYGDSALTHFSQAHVCVVGVGGVGSWA
                     VEALARTGIGRLTLIDLDNVAESNVNRQLHALTGDFGKAKVTALRERITQINPQCEVF
                     EIEDFVTEDNLPEYFGKGFDFVIDAIDQVRVKAAMAAYFVERKQPFVLSGGAGGQKNP
                     ALIQTADLSRVTHDPLLANLRYTLRKRYGFSRDTKEKMRVPCVYSTENIMPPQSGAAC
                     SADAAPQGLSCAGYGASMLVTASFGLYCAQAAVEHIAGKK"
     gene            4154..5008
                     /locus_tag="WNA25_001072"
     CDS             4154..5008
                     /locus_tag="WNA25_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFA9823838.1"
                     /translation="MPPIPALSAPSEHTVAWVFGQPVTDLPQDLFIPPDALKVVLGSF
                     QGPLDLLLYLIRKQNIDVLDIPMVEITGQYLHYIAQMEAYQFDLAAEYLLMAAMLIEI
                     KSRLLLPRTEAVEDEEADPRAELVRRLLAYEQMKLAAQGLDALPRAGRDFAWAYLPLE
                     IAAETKLPEVYIADLMQAWLGILSRAKHTRSHEVIQETLSVRAQMTAILRRLNEHGIC
                     RFHDLFNPEQGAAYVIVNFIALLELAKEGLVGIVQEDGFGEIRISLNHEGAHSDGIFG
                     TRGGRDVF"
     gene            5049..6257
                     /gene="pncB"
                     /locus_tag="WNA25_001073"
     CDS             5049..6257
                     /gene="pncB"
                     /locus_tag="WNA25_001073"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFA9823839.1"
                     /translation="MTGIIHSLLDTDLYKFTMLQVVLHQFPQTHSLYEFRCRNVSTVY
                     PLADIREDLEAELDALCRLRFTHDELGYLRSLRFIKSDFVDYLELFQLQRRFVEVGTD
                     DKGRLNIRIEGPMIQAMFFEIFILAIVNELYFRRLETPAVIEEGERRLQAKAARLKEI
                     AAAQNPDEPPFLISDFGTRRRYKLAWQEHVIRTLLEAAPSIVRGTSNVYLAKKLGITP
                     IGTMAHEFLQAFQALDVRLRNFQKAALESWVHEYRGDLGVALTDVVGMDAFLRDFDLY
                     FAKLFDGLRHDSGDPYVWGDKAYAHYQKLKIDSRTKMLTFSDGLDIERSWALHQYFKG
                     RFKTGFGIGTNLTNDMGHTPLNIVLKLVECNGQSVAKLSDSPGKTMTNNSTFLAYLRQ
                     VFGIPEPRTP"
     gene            6349..8067
                     /gene="argS"
                     /locus_tag="WNA25_001074"
     CDS             6349..8067
                     /gene="argS"
                     /locus_tag="WNA25_001074"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFA9823840.1"
                     /translation="MNLHQTVEHEAAAAFAAAGIAGSPVVLQPTKNAEHGDFQINGVM
                     GAAKKAKQNPRELAQKVADALAGNAVIESAEVAGPGFINLRLRHEFLAQNIHAALNDA
                     RFGVAKTAQPQTVVIDYSSPNLAKEMHVGHLRSSIIGDSISRVLEFTGNTVIRQNHVG
                     DWGTQFGMLVAYLVEQQKDNAAFELADLEQFYRAAKVRFDEDPAFADTAREYVVKLQG
                     GDETVLALWKQFVDISLSHAQAVYDTLGLKLRPEDVAGESKYNDDLQPVVDDLVQKGL
                     AVEDDGAKVVFLDEFKNKEGEPAAFIVQKQGGGFLYASTDLACLRYRIGRLKAGRLLY
                     VVDHRQALHFEQLFTTSRKAGYLPEDAKAEFIGFGTMMGKDGKPFKTRSGDTVKLVDL
                     LTEAVERATALVKEKNPELGADEAAKIGKTVGIGAVKYADLSKNRTSDYVFDWDAMLS
                     FEGNTAPYLQYAYTRVQSVFRKAGEWDATAPTVLTEPLEKQLAAELLKFENVLQSVAD
                     TAYPHYLAAYLYQAATLFSRFYEACPILKAEGASRNSRLQLAKLTGNTLKQGLDLLGI
                     DVLDVM"
     gene            8619..10037
                     /locus_tag="WNA25_001075"
     CDS             8619..10037
                     /locus_tag="WNA25_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFA9823841.1"
                     /translation="MNIHALLSKQWTLPPFLPKRLLLSLLILLVPNAVFWVLALLTAT
                     ARPIVNLDYLPAALLIALPWRFVKIAGVLAFWPAVLFDGLMMVIQLFPFMDLIGAINL
                     VPFILTAPAPYQIMTGLLLLYMLAMPFVLQKAAVKTDFRHIAVCAAVVAAAGYFTGHL
                     SYYDRGRMANIFGANNFYYAKSQAMLYTVSQNADFITAGLVDPVFLPLGNQQRAATRL
                     SEPKSQKILFIVAESWGLPGNPELQNATFAKLLAQKDRFSVWESGSFPFIGATVEGEM
                     RELCAYGGLRGFALRRAPDEKFARCLPNRLKQEGYATFAMHGAGSSLYDRFSWYPRAG
                     FQKIKTAENLIGKKTCAIFGGVCDSELFGEVSAFFKKHDKGLFYWMTLTSHADYPESD
                     IFNHRLKCTEYGLPAETDLCRNFSLHTQFFDQLADLIRRPEMKGTEVIIVGDHPPPVG
                     NLNETFRYLKQGHVAWLHFKIK"
     gene            10043..10303
                     /locus_tag="WNA25_001076"
     CDS             10043..10303
                     /locus_tag="WNA25_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823842.1"
                     /translation="MPSERTNSLQTAFCRQTDLLKPTFFIISDKLLCKNPKIQNHRKL
                     SKKQKPSAVVPAKAGIQTRKPQKFIGKTETRKQRTEFPPAQE"
     gene            complement(10490..11236)
                     /locus_tag="WNA25_001077"
     CDS             complement(10490..11236)
                     /locus_tag="WNA25_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFA9823843.1"
                     /translation="MDFRFDIIYEYRWMFLYGALTTLGLTVVATAGGSVLGLLLALAR
                     LIHLEKAGAPMRVLAWALRKVSLLYVTLFRGTPLFVQIVIWAYVWFPFFVHPSDGILV
                     SGEAAIALRRGYGPLIAGSLALIANSGAYICEIFRAGIQSIDKGQMEAACSLGLTYPQ
                     AMRYVILPQALRRMLPPLASEFITLLKDSSLLSVIAVAELAYVQNTITGRYSVYEEPL
                     YTAALIYLLMTTFLGWIFLRLEKRYNPQHR"
     gene            complement(11415..12086)
                     /locus_tag="WNA25_001078"
     CDS             complement(11415..12086)
                     /locus_tag="WNA25_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFA9823844.1"
                     /translation="MQIKKIMKWLPVALSLLGALGYTGYDSEAVRTAVAVLDVLGTAG
                     DVGSDAPVRRRASAKSGHSYTGTVSKVYDGDTLHVIDGDGAKHKIRMAYIDAPEMKQA
                     YGTRSRDNLRAAAEGRKVSVRVFETDRYQREVAQVSAGKTDLNLMQVQDGAAWHYKSY
                     AKEQQDKADFADYADAQIQAERERKGLWKAKNPQAPWAYRRAGRSGGGNKDWMDSVGE
                     WLGIW"
     gene            complement(12155..12826)
                     /gene="rpiA"
                     /locus_tag="WNA25_001079"
     CDS             complement(12155..12826)
                     /gene="rpiA"
                     /locus_tag="WNA25_001079"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFA9823845.1"
                     /translation="MTTQDELKRIAAEKAVEFVPENEYIGIGTGSTINFFIEALGKSG
                     KKIKGAVSTSKKSGELLARYDIPVVSLNEVSGLAVYIDGADEVNHALQMIKGGGGAHL
                     NEKIVASASEKFVCIADESKYVSRLGKFPLPVEAVESARSLVSRKLLAMGGQPELRIG
                     YTTFYGNQIVDVHGLNIDQPLTMEDEINKITGVLENGIFARDAADVLILGTEEGAKVI
                     YPCQG"
     gene            complement(12903..13385)
                     /locus_tag="WNA25_001080"
     CDS             complement(12903..13385)
                     /locus_tag="WNA25_001080"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFA9823846.1"
                     /translation="MTNIRIGQGYDVHQLIEGRKLILGGVEIPFEKGLLGHSDADALL
                     HAVTDALLGAAGLGDIGSHFPDTAAEFKDADSRVLLRAAYQSVQAQGWQAVNVDTTVI
                     AQKPKLAPHIPQMRANIAADLGIDISCVNIKGKTNEKLGYLGRMEGIEAQAAVLLVRI
                     "
     gene            complement(13417..14106)
                     /locus_tag="WNA25_001081"
     CDS             complement(13417..14106)
                     /locus_tag="WNA25_001081"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFA9823847.1"
                     /translation="MKRKNIALIPAAGIGVRFGADKPKQYVEIGSKTVLEHVLGIFER
                     HEAVDLTVVVVSPEDTFADKVQTAFPQVRVWKNGGQTRAETVRNGVAKLLETGLAAET
                     DNILVHDAARCCLPSEALTRLIEQAGNAAEGGILAVPVADTLKRAESGQISATVDRSG
                     LWQAQTPQLFQAGLLHRALAAENLDGITDEASAVEKLGVRPLLIQGDARNLKLTQPQD
                     AYIVRLLLNAV"
     gene            complement(14103..14837)
                     /gene="dnaQ"
                     /locus_tag="WNA25_001082"
     CDS             complement(14103..14837)
                     /gene="dnaQ"
                     /locus_tag="WNA25_001082"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFA9823848.1"
                     /translation="MMTRQIILDTETTGLYADGGDRLVEFAGLEMVNRQMTDKNLHLY
                     VHPERDMPEEAARVHGLTIQVLEEKNAPPFAEVGRQIADFLRGAELIIHNAKFDVGFL
                     NMEFRRMGLPTVEELGCTVTDTLAMAREMFPGQKASLDALCNRFSVDRSKRVLHGALI
                     DCELLGEVYLAMTRRQFDLMGETEEEEPTAKPVASAEMKLGAKLKVIKADEAELAAHE
                     EYLDGLGEACIWRKEAVPSENGGENA"
     gene            complement(14993..16303)
                     /locus_tag="WNA25_001083"
     CDS             complement(14993..16303)
                     /locus_tag="WNA25_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFA9823849.1"
                     /translation="MDAKKGGLGLVKSRRFGPLFATQFLGAFNDNVFKTALFVMIGFY
                     GLGQNGVLPAGQMLNLGALLFILPYFLFSALSGQLGNKFDKAVLARWVKVLEIIIMAV
                     AAYGFYIRSAPLLLLCLFCMGAQSTLFGPLKYAILPDYLDDNELMMGNSLIESGTFVA
                     ILFGQILGTAVAGAPPYIVGILVLLVAVGGTAGSLFMPSVPAKAADTQIEWNIVRGTK
                     SLLRETVRHNPVFTAIIGISWFWFVGAVYTTQLPTFTQIRLGGNDNVFNLMLALFSIG
                     IAAGSVLCAKFGRERLMLAWVTVGALGLTVCGLVLVWLTHGHRFEGLNGIFWFLSQGW
                     AYSVMAVMTLIGFFGGFFSVPLYTWLQTASSETFRARAVAANNIVNGIFMVSAAVLSA
                     VLLFLFDSISLLYLIVALGNIPLAVFLIKRERRFLGAAAIRKKP"
     gene            complement(16293..16478)
                     /gene="ccoS"
                     /locus_tag="WNA25_001084"
     CDS             complement(16293..16478)
                     /gene="ccoS"
                     /locus_tag="WNA25_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HFA9823850.1"
                     /translation="MESMFILVPISIILAFVIGWFFWWSGKNGQFDDLEGPAHRILMD
                     DDSTSKLIEKEKENDGR"
     gene            complement(16478..16777)
                     /locus_tag="WNA25_001085"
     CDS             complement(16478..16777)
                     /locus_tag="WNA25_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823851.1"
                     /translation="MHYFSIHDQSGRHIGFFILLADDESEARLQSGRFAVKLEDGTGN
                     HVLSPFGQTEIPQYWRVVKDRIELFFDEAPVGTLRNEYLTVSGQTFVLNDLIGNM"
     gene            17128..18324
                     /locus_tag="WNA25_001086"
     CDS             17128..18324
                     /locus_tag="WNA25_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFA9823852.1"
                     /translation="MSQKLILVLNCGSSSLKGAVLDNGSGEVLLSCLAEKLNLPDAYI
                     TFKVNGEKHKVDLSAHPDHTGAVEALMEELEAHGLDSRIGAIGHRVVSGGELYNESIL
                     VDDEVIAGIEKCIPLAPLHNPAHLLGLRAAQSIFKGLPNVVVFDTSFHQTMPEVAYKY
                     AVPQELYEKYGLRRYGAHGTSYRFVADETAHFLGKDKKDLRMVIAHLGNGASITAVAN
                     GESRDTSMGLTPLEGLVMGTRSGDIDPSVFGFLAENANMTIAQITDMLNKKSGLLGIS
                     GLSNDCRTIEEEAAKGHKGAKLALDMFIYRLAKYIGSMAVAAGGLDALVFTGGIGENS
                     DIIRERVIGYLGFLGLNIDQEANLKARFGNAGVITTADSKAVAVVIPTNEELMIAHDT
                     ARLSGL"
     gene            complement(18374..18496)
                     /locus_tag="WNA25_001087"
                     /pseudo
     CDS             complement(18374..18496)
                     /locus_tag="WNA25_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(18838..20679)
                     /locus_tag="WNA25_001088"
     CDS             complement(18838..20679)
                     /locus_tag="WNA25_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFA9823853.1"
                     /translation="MRTNYLSETDSIMKKLICLFAVFLMLCGRAFALDANDLLPPEKA
                     FVPELTVADDGVNVRFRIADGYYMYQAKIVGKTDPADLLGQPSFSKGEEKEDEFFGRQ
                     TVHHHEAQVAFPYAKAVGEPYKLVLTYQGCAEAGVCYPPVDTEFDISGNGTYHPQTDE
                     PASAKDRFLQPSSQNGSGALPPPKGDEGGDGRFKLSWDTLNANLLAFFLAGLGLSFTA
                     CMYPLLPIVSSIVVGDKKAGKARAFVLSVVYVQGLALTYTLVGIVAGLTGALLTVWLQ
                     QAWVVLAASALMVVLALSMFGLFNIQLPNAVQSYFQNQSSRLSGGKIVSVFIMGILSA
                     LIVGPCVAPPLAFALGYIGQTGDAVLGGLALYTLALGTGVPLIAIGTFGGHILPKAGD
                     WMNAVKYAFGFILLAVAVYLATPHLPYYLVVALYTLLMLVPAFMLLVNGRRQKRRPKA
                     VAFALGSILLIGGAWFGWQGANGKTTALHHFLTLNPPAEAGKFSEHGKMFADTAALKA
                     AMDTALKEHPDKPVVLDFYADWCISCKEMAVYTLNQPEVHQAVDMERFFQIDVTANTP
                     EHQALLKEYGLFGPPGVFVVRADGSRSEPLLGFVKADKFIEWYEQNR"
     gene            20770..21240
                     /locus_tag="WNA25_001089"
     CDS             20770..21240
                     /locus_tag="WNA25_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HFA9823854.1"
                     /translation="MYDVNTHDVRRFFARVWQQRLNPLQLGALEQKALRIVEAHPEYH
                     RYLERIEDHLDTDWLPENGESNPFLHMSLHLSVQEQAGIDQPHGIRAIHDTLCAKRGW
                     PEAEHEMMEALAETLWTAQRYGTGLDVNFYMTRLRKLIGLGAEDQARLNPHEIA"
     gene            complement(21313..22125)
                     /gene="hpnC"
                     /locus_tag="WNA25_001090"
     CDS             complement(21313..22125)
                     /gene="hpnC"
                     /locus_tag="WNA25_001090"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HFA9823855.1"
                     /translation="MSVGHYENFPVGSLVLPRRLRKPVHAVYAFARTADDMADEGSMP
                     SEARLSGLEGLRRELDVLASGGRSAHPLIARLDAEAVVPFGLDLQPFYDLLSAFSQDV
                     VKTRYAHFGDLTDYCRRSANPVGRIMLALYGKTDAVCVAQSDGICTALQLVNFWQDVA
                     VDWQKGRVYIPQDDLLKFGVSEEQIAAGRADAAFQRLMAYECRRAFRMLKAGSPLARE
                     LKGRIGLELRMIVLGAQLILQKLDACRYDVFAQRPVLDKKDWLIMLKRALWK"
     gene            complement(22212..22694)
                     /locus_tag="WNA25_001091"
     CDS             complement(22212..22694)
                     /locus_tag="WNA25_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFA9823856.1"
                     /translation="MAIAKNSVVSLHYEMYDANNQLLDKTEEPIAYLHGGYDGIFPLV
                     EEALHGKDAGDTVDVALSSDDAFGEQDSELVRIEDAGAFPVEVEVGMMFEADDPETGD
                     VVVYRVTDVADGKAVVDGNHPLAGMKIRFKATVESVRDASDEEIAHGHVHGSHGHYHH
                     "
     gene            23167..>23318
                     /locus_tag="WNA25_001092"
     CDS             23167..>23318
                     /locus_tag="WNA25_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823857.1"
                     /translation="MKKSLFAAALLSLALAACGGEKAAEAPAAEASSTEAPAAEAPAA
                     EAPAAEA"
BASE COUNT         5570 a         6890 c         5671 g         5187 t
ORIGIN      
        1 gaatccgttt tttgagtttc gcttaagttc aataaatcct gaaactttaa tctcgccgtg
       61 aaaatgaatt ttctatttca atttgtcata tatagttaat ttttttaggg tattttattt
      121 tttgttccaa agtcaaaata tgccgtccga acgttcgggc ggcagacaaa acggcactgc
      181 ccgataaagg cagtgccgtt gtccgtttta aaccgtgaaa catcagccca aatcaaaggc
      241 tttatgcaat accctggttg ccagttccat gtatttttcg tcaatcaata cggaaacttt
      301 gatttcggag gtggaaatca tttggatgtt gatgccctct tcggcgagcg tgcggaagat
      361 tttggcggct acgccgacgt gcgaacgcat acccaaaccg actgcggaga ctttgcacac
      421 ggtgtcgtcg ccgtcgatag aagctgcgcc gatactgtct ttacgttccg acaggatttc
      481 caaagtctgt ttgtaatcgc cgcgcggtac ggtgaaagaa aaatcggttg tgccttcgct
      541 gccgacattt tggataatca tatcgacttc gatgttggca tctgcgaccg cgcccaaaat
      601 ctgataggcg acgccgggct tatcgggcac accgcgcacg ttgatgcggg cttggttttt
      661 atcgaatgcg ataccggtta cggcagctct ttccatgttg tcgtcctctt caaaggtaat
      721 taaggtgccg ttgccgccgt cttgcaggct gctcagtacg cgcaggcgca ctttgtattt
      781 tccggcgaat tctactgaac ggatttgcaa aactttcgaa ccgaggctgg ccagttcgat
      841 catttcttca aatgtaaccg tatccatacg gcgcgcttcg ggtacgacgc gggggtcggt
      901 ggtgtaaacg ccgtctacgt cggtatagat ttggcattcg tccgctttga gggctgcggc
      961 aagcgcaacg gcggaagtgt cggaaccgcc gcgtcctagc gtggaaatat tgccttcact
     1021 gctgatgcct tggaagccgg caacgatgac gactttgccg gcggcgaggt cggcgcgcat
     1081 tttttcgtca tcaatgcttt ctatgcgggc tttggtgtgg gcggtatcgg ttttgaggga
     1141 gacctgccag cctgtgtagc ttttggcatc cacgccgata tccttcaatg ccattgccaa
     1201 aaggccgatg gttacttgtt cgccggtagc taagacgacg tccagctcgc gcggatcggg
     1261 atgctcctgc atttcgtgcg ccaacgcaac cagacggttg gtttcgccgc tcatggcgga
     1321 tacgacgacg acgatatcgt gtccttcggc gcgggcttta gcgacacgtt tggccacgtt
     1381 tttaatgcgc tcgggcgagc ctacggatgt gccgccgtat ttatgtacga ttaacgccat
     1441 gtttcatgct ttcttgtgtg ggattggtcg ggcagcatgg tttgctggaa aagcggctat
     1501 tattactatt ttttacatga agttcaagaa cggactgcgc tttcccgcct gccgtttgac
     1561 agcggtcagc gaaaaacctg ttctttcaga tttttgacaa aatgccgtct gaacggtttt
     1621 cagacggcat acggacagta attaagcaac ggacaacgca ttttgctgat aacgcagcag
     1681 ttcgcctatg cctttttgcg ccagtgcaac cagtttgccc aactcgtcca aactgaatgg
     1741 cgcgccttcc gccgttccct gtatttcgat gattttcccc gatgcggtca tgacgatgtt
     1801 cacatcactg tcgcagccgg agtcttcggg ataatccaaa tccaaaagcg gcacgccgtt
     1861 cactacgccc gctgatacgg cggcaacagc ttcgaggatg gggttttcac tcaaaatgcc
     1921 gtctgaaacc agtttgccga cggcgatttg cagcgcgaca aacgcaccgg taatcgaagc
     1981 cgtacgcgtg ccgccgtccg cctgaatcac atcgcaatca atcaggattt gtcgttcgcc
     2041 gagtttttcc atatccacga ccgcgcgcag cgaacgcccg atcaagcgtt ggatttcttg
     2101 tgtgcgtccc gactgtttgc ccgccgaagc ttcgcggcgc atacgcaaag cggttgaggc
     2161 aggcagcata ccgtattccg ccgttaccca gccttggttt ttgccgtgca gaaacggtgg
     2221 ggcattttcg tccacggaag cggtacaaat cactttggta ttaccgcatt caataaggca
     2281 cgaaccgtcc gtatgcggca ggaaatgagg ggtgattttg atatcgcgca ggctgtcggc
     2341 ggcgcgtgag gtgcggatat aatcaggcat actgccctcc cgttaaaaac agataaatta
     2401 aaaagcctta aatatgaaaa atcacattta aggccttcaa actgaaaatt tctacgcctc
     2461 ttcggctttg ctacggataa tcaaaagcgg caggtggctt tggcgcatta ccgtttcggc
     2521 gaaactgccc atcaaaaggt gcatcagccc ggtgcgcccg tgcgtgccca acaccagcag
     2581 gccggcaccg ttttcatcgg cataatcgac caaatcctgc gccatttcgc gcgcaccctt
     2641 attggcaacc agcaggtgtt tgacggtatt tcccacaccc agttcccggg cggtgcgctc
     2701 ggcggcatcc aaaacctcgt tgccttgcgc gacggcggcg gcctcgtaac tttcgtgttg
     2761 caaaaattcg ggggcgagcg ccatatattc ggcagggttg gcaacgtgta ccaaagtcag
     2821 acgcgcaccg ttgaccccgg caagctcggc ggcgtgtttc agggcattga tggacgtttc
     2881 actgccgtca acggcaacga ccagatgttt gtacatatcg tattctcctt ttgcaccgtc
     2941 tcacggtgcc gtctcttcag gtttgcgcaa aggcagtctg cgccgtttca ttatagaccc
     3001 gccgtcgggc tttatacaac agccgaacag cctgactgct ttccagtata atatgccgct
     3061 ttcgtgcagt caggcatttt ttgccggctt tcgttcactt tttgatttga cgcaatcttg
     3121 caggattcga ccatgtccga caacgctttg acctcttcgc gacgcttcgg cggcatcgcc
     3181 agactctacg gagactctgc cttgacgcac ttttcacagg cacacgtctg cgtggtcggc
     3241 gtgggcgggg tcggctcgtg ggcggtcgag gctttggcgc gcacgggcat cgggcgtttg
     3301 actttgattg atttggacaa cgttgccgaa tcgaatgtca accgccagct gcacgccctg
     3361 accggcgact tcggcaaagc aaaagttacc gccttgcgcg aacgtattac acaaatcaat
     3421 ccgcaatgcg aagtatttga aattgaagat ttcgttaccg aagacaattt gccggaatac
     3481 ttcggaaaag gttttgattt cgtcatcgac gcgatcgacc aagtgcgcgt caaagcggca
     3541 atggcggctt attttgtgga acgcaaacaa ccgtttgtcc tcagcggcgg cgcgggcgga
     3601 cagaaaaatc ccgccctgat tcaaaccgcc gatttaagcc gcgtaaccca cgacccgctg
     3661 cttgccaacc tgcgctacac cttgcggaaa cgctacggct tcagccgcga tactaaggaa
     3721 aaaatgcgcg tgccgtgcgt gtattcgacc gaaaatatca tgccgccgca atcgggcgca
     3781 gcgtgttctg ccgatgccgc gccgcagggc ttgtcatgcg ccggctatgg cgcaagtatg
     3841 ctcgttaccg cttcgttcgg actgtattgc gcacaggcgg cggtggaaca catcgcgggc
     3901 aaaaaataag caatgccgtc tgaaacagga ttcagacggc atttgaacaa acgcggcaat
     3961 caagctgtcg gttcagaaaa accaacatcg gcacgcctaa aaccgtattc acaatctact
     4021 ccttttcaaa atatttgcat ttaaaagccg ttataatgcc gtccgaacat ctgcccgacc
     4081 acattatgcg tgaatgccgg cagattgttt tcttttgtaa acttatatta aaattcactt
     4141 accaattcac gccatgccgc ccatccctgc cctatccgca ccatccgagc acactgtcgc
     4201 atgggtattc ggccaacccg ttaccgattt gccgcaggat ttgtttatcc cgcccgacgc
     4261 gctgaaagtc gtattgggca gttttcaagg ccctttggat ctgctgctct acctgattcg
     4321 caaacagaat atcgacgtac tggatattcc gatggtggaa attaccgggc agtatctgca
     4381 ctatattgcc caaatggaag cctatcagtt tgatttggcg gcggaatatc ttttgatggc
     4441 ggcaatgctg attgaaatca aatcgcgcct gctgctgccg cgtaccgaag ccgtcgaaga
     4501 cgaagaggcc gacccgcgtg ccgagttggt gcgccgtctg cttgcctacg agcaaatgaa
     4561 actggcggcg cagggtttgg acgcgctgcc gcgtgcggga cgggatttcg cgtgggctta
     4621 cctgccgctg gaaattgcag ccgagacgaa gctgcccgag gtttacatcg ccgatttgat
     4681 gcaggcatgg ttgggcattc tttctcgggc aaaacatacg cgcagccacg aagtaatcca
     4741 agaaaccctt tccgtgcgcg cgcaaatgac ggcaatcctg cgccgtttga acgaacacgg
     4801 gatatgcagg tttcacgacc tgttcaatcc cgaacagggc gcggcttacg tgatcgtcaa
     4861 cttcatcgcc ctgttggagc ttgccaaaga aggattggtc ggaatcgtac aggaagacgg
     4921 tttcggagaa atccgaatca gcctcaatca tgagggggcg cattcagacg gcattttcgg
     4981 cacacggggc gggcgcgatg tgttctaata cgccccaagc cgccaccaga aatcgggaga
     5041 cacgccatat gaccggcatc atacattcgc tgctcgacac tgacctctac aaattcacta
     5101 tgctgcaagt agtcctgcac cagtttccgc agacgcacag cctttacgaa ttccgctgcc
     5161 gcaacgtctc gaccgtctat ccgcttgccg acatcaggga ggacttggaa gccgaactcg
     5221 acgcgctctg ccggctgcgc ttcacccacg acgaactcgg ctatctgcgc tccctgcgtt
     5281 tcatcaaaag cgactttgtc gattatctcg aactcttcca gctccaacgc cgctttgtcg
     5341 aagtcggcac agacgataaa ggccgtctga acatccgtat cgaaggcccg atgatacagg
     5401 cgatgttttt tgaaatcttc atcctcgcca ttgtcaacga actttacttc cgccggctgg
     5461 aaacccctgc cgttatcgaa gaaggcgaac gccggcttca agccaaagcc gcgcgcctga
     5521 aagaaatcgc cgccgcacag aatccggacg aaccgccctt cctgatttcc gacttcggca
     5581 cgcgccgccg ctacaagctc gcgtggcagg aacacgtcat ccgcaccctg ctcgaagccg
     5641 ccccaagcat cgtgcgcggc acaagcaacg tttacctcgc caaaaaactc ggcatcaccc
     5701 ccatcggcac catggcgcac gaattcctcc aagccttcca agccctcgac gtgcgcctgc
     5761 ggaatttcca aaaggccgcg ctcgaaagct gggtgcacga ataccggggc gatttgggcg
     5821 ttgccctgac cgacgtggtc ggtatggatg ccttcctgcg cgatttcgac ctctatttcg
     5881 ccaaactctt tgacgggctg cgccacgaca gcggcgaccc ttacgtttgg ggcgacaaag
     5941 cctacgccca ctatcaaaag ctcaaaatcg acagccgcac caaaatgctg accttctccg
     6001 acgggctgga catcgaacgc tcttgggcat tgcaccaata tttcaaaggc cgcttcaaaa
     6061 ccggcttcgg catcggcacc aacctcacca acgatatggg gcatacgccc ttgaatatcg
     6121 tgttgaaact ggtcgaatgc aacgggcagt ccgtcgccaa gctctccgac tcgccgggca
     6181 aaaccatgac caacaacagc accttcctcg cctacctgcg ccaagtgttc ggcatacccg
     6241 aacctagaac gccgtaaacc ggcagaaaaa gcgtacaatt cctgtttccg ccgcataaaa
     6301 tcttttaaaa taccgcctga tttgaattta accgaaagac cgaacttcat gaacctacat
     6361 caaaccgtcg aacacgaagc cgccgccgcc tttgccgccg caggcatcgc cggcagcccc
     6421 gttgtcttgc agccgaccaa aaatgccgaa cacggcgatt tccaaatcaa cggcgtgatg
     6481 ggtgcggcga aaaaagccaa acaaaatccg cgcgaactgg cgcaaaaggt cgccgacgca
     6541 ttggcgggca acgccgtcat cgaaagcgcg gaagtggccg gacccggctt tatcaacctg
     6601 cgcctgcgcc acgaatttct cgctcaaaac attcatgcgg ctttgaacga cgcgcgtttc
     6661 ggcgtagcga aaactgccca accgcaaacc gtcgttatcg actattcttc gcctaatctg
     6721 gcgaaggaaa tgcacgtcgg ccatctgcgc tccagcatca ttggcgacag catttcacgc
     6781 gtgttggaat ttacgggcaa caccgtcatc cgccaaaacc atgtcggcga ctggggtacg
     6841 cagttcggca tgttggtcgc ttatttggtc gagcagcaaa aagacaatgc cgcgttcgag
     6901 ctggcggatt tggagcagtt ttaccgcgcc gccaaagtgc gctttgacga agaccccgcc
     6961 tttgccgaca ccgcacgcga atacgttgtg aagctgcaag gcggcgatga aaccgtgttg
     7021 gcgttgtgga aacagtttgt cgatatttcg ctctcgcacg cccaagccgt ttacgacacg
     7081 ctgggcttga aactgcgtcc tgaagacgtg gcgggcgaat ctaaatacaa cgacgatttg
     7141 cagcccgtgg ttgatgattt ggttcaaaaa ggcctggcgg ttgaagacga cggcgcaaaa
     7201 gtcgtgttct tggacgagtt taaaaacaaa gaaggcgaac ccgccgcatt tatcgtgcaa
     7261 aaacaaggcg gcggcttcct ttatgcctcc accgatttgg cgtgcctgcg ctaccgcata
     7321 ggccgtctga aagccggccg cctgctgtac gtcgtcgacc accgccaagc cctgcacttc
     7381 gaacaacttt tcaccacttc ccgcaaagca ggctatctgc ctgaagatgc aaaagccgag
     7441 tttatcggct tcggcaccat gatgggcaaa gacggcaaac cgttcaaaac gcgcagcggc
     7501 gacaccgtaa aactggttga cctgctgacc gaagccgtcg agcgcgccac cgctttggtg
     7561 aaagaaaaaa atcccgaatt gggcgcggat gaagccgcca aaatcggcaa gaccgtcggc
     7621 atcggcgcag tcaaatacgc cgacttgagc aaaaaccgca ccagcgatta tgtgttcgac
     7681 tgggacgcca tgctctcatt tgaaggcaac accgccccct acctgcaata cgcctacacc
     7741 cgcgtgcaaa gcgtgttccg caaagccgga gaatgggacg caaccgcacc gaccgttttg
     7801 accgaaccac tggaaaaaca gcttgccgcc gagcttctga aatttgaaaa cgtgctgcaa
     7861 agcgtggcgg acaccgcgta tccgcactac ctcgccgcct acctttacca agccgccacc
     7921 ctgttcagcc gcttctacga agcttgcccg attttgaaag ccgaaggcgc aagccgcaac
     7981 agccgcctgc aactggcaaa actcaccggc aacacgctga aacaaggctt ggatttgctg
     8041 ggcatcgatg tgttggacgt aatgtaaaac cgcaccgccc gattgcggac aacagccctg
     8101 caatcaaccg gcggaccatc ctcatccgaa tctgaaaaaa gcggcgcgat acaccgtatc
     8161 cgccgcccct cccaaaatgc gaaacagaca aacgccaagc aagcaagcaa gcaagcaagc
     8221 aagcaaaaaa ttataacccc ccttcctgcc gacgcacgca ctttccgcgc ggcgtattcc
     8281 ccttttcccg cccctcaaac ccgccttttc ttcaggcagg gtttcagccc gcctcctttc
     8341 cctgttttcc ttccctcaac acgcgtgctc gccccctgcc gcaccgtgcc gcactttcgc
     8401 gcccggacgg catcgttccg ccatccggtt ctctgtttta aatacccctg tttcagaaaa
     8461 aaatgcagat gtttcaacac acaggacgac atataaagca ccgccctatg tgtcgtcctg
     8521 atttggaagg ggttacaccc ttcctaaata tagtctgatc ctgccgcccc gaaggacaga
     8581 tgtccgagtg gcggggtttc aaccgaaaag gaaatacgat gaatattcac gccctgctct
     8641 ccaaacaatg gacgctgccg ccattcctgc cgaaacggct gctgctgtcc ctgctgatac
     8701 tgctggtccc caatgcggtg ttttgggttt tggcactgct gaccgccacc gcccgcccga
     8761 ttgtcaattt ggactacctt cccgccgcgc tgctgatcgc cctgccttgg cgtttcgtca
     8821 aaattgccgg cgtattggca ttttggccgg cggttttgtt tgacggactg atgatggtca
     8881 tccagctctt cccttttatg gacctcatcg gcgccatcaa cctcgtcccc ttcatcctga
     8941 ccgcccccgc cccttatcag ataatgaccg ggctgttgct gctgtatatg ctggcgatgc
     9001 cgtttgtgtt gcaaaaagcc gccgtcaaaa ccgacttccg acacattgcc gtctgtgccg
     9061 ccgttgtggc ggcagccggc tatttcaccg gccatttgag ttactacgac cgggggcgga
     9121 tggccaatat cttcggcgca aacaacttct attacgccaa aagtcaggcg atgctctaca
     9181 ccgtcagcca gaatgccgac tttattaccg ccggcctggt cgaccccgtc ttcctcccct
     9241 tgggcaatca gcagcgtgcc gccacgcggc tgagtgagcc gaaatctcaa aaaatcctct
     9301 ttatcgtcgc cgaatcttgg gggctgccgg gcaatcccga gcttcaaaac gccacttttg
     9361 ccaaactgct ggcgcaaaaa gaccgttttt cggtttggga aagcggcagt tttcccttca
     9421 tcggcgcgac ggtcgaaggc gaaatgcgcg aattgtgcgc ctacggcggt ttgcgcgggt
     9481 tcgcactgcg ccgcgcaccc gacgaaaaat ttgcccgctg cctccccaac cgtttgaaac
     9541 aagaaggtta cgccaccttt gcgatgcacg gcgcgggtag ttcgctttac gaccgcttca
     9601 gctggtatcc gagggcgggc tttcaaaaaa tcaaaaccgc cgaaaacctg atcggtaaaa
     9661 aaacctgcgc cattttcggc ggcgtgtgcg acagcgagct gttcggcgaa gtgtcggcat
     9721 ttttcaaaaa acacgacaag ggactgtttt actggatgac gctgaccagc cacgccgact
     9781 atcccgaatc cgacattttc aaccacaggc tcaaatgcac cgaatacggc ctgcccgccg
     9841 aaaccgacct ctgccgcaat ttcagcctgc acacccaatt cttcgaccaa ctggcggatt
     9901 tgatccgacg ccccgaaatg aaaggcacgg aagtcatcat cgtcggcgac catccgccgc
     9961 ccgtcggcaa cctcaatgaa accttccgct acctcaaaca gggacacgtc gcctggctgc
    10021 acttcaaaat caaataacaa caatgccgtc tgaacgcacc aacagccttc agacggcatt
    10081 ttgcagacag accgacctcc tcaagcccac ttttttcatc atctccgata aattgctttg
    10141 taagaatcca aaaatccaaa atcacaggaa gttatcaaaa aaacagaaac catccgccgt
    10201 cgttcccgcg aaagcgggaa tccagacccg gaaaccgcag aaatttatcg gaaaaaccga
    10261 aactcgaaaa caacggacag aattcccgcc tgcgcaggaa tgacggctca tacatcaccc
    10321 aaaaaaaatt gaaaccaaac agctgggatt ccctcttgcg tgaggctgac agatgccgtc
    10381 tgaaagactt tcagacggca tagggcgttt tctctttgaa tttaaatttt gccacaaaaa
    10441 agccgcccca aatgaatacc cgggcagctt tttgttgata tgactccaat cagcggtgtt
    10501 gcggattgta acgtttttcc aaacgcagga atatccagcc taagaaagtc gtcatcaaca
    10561 gataaatcag ggcggcggtg taaagcggtt cttcataaac cgaataccgg cccgtaatcg
    10621 tattctgaac atacgccaac tccgccacag caatgaccga cagcagcgag ctgtctttca
    10681 agagcgtgat gaactcgctc gccaaaggcg gcagcatacg gcgcaatgcc tgcggcagaa
    10741 tcacatagcg catcgcctgc ggataggtca gtcccaaaga acacgccgcc tccatctgtc
    10801 ctttgtctat agactggatg cccgcgcgga aaatctcaca gatatacgcc cccgagttgg
    10861 cgatcagtgc caaagaaccg gcaatcagcg gcccgtatcc gcgacgcagc gcgattgccg
    10921 cctcgccgct gaccaaaatg ccgtctgaag gatggacgaa aaacggaaac cacacatacg
    10981 cccaaatcac aatctgcaca aacagcggcg taccccggaa cagggtaacg tacagcagcg
    11041 aaaccttacg caacgcccac gccagcacgc gcatcggcgc accggctttt tccaagtgaa
    11101 tcaggcgcgc caacgccaac aacagaccca ataccgaacc gcccgccgtc gccacgaccg
    11161 tcagccccaa ggtcgtcagt gcgccgtaaa gaaacatcca gcggtattcg taaataatgt
    11221 caaaacgaaa atccataaac cgtccgtatc aaaaaccggc ggaactgccg ccgttgcaaa
    11281 ataatccgcc attttaccgt aaaaaccgcc gcctgaactt ttttatcgcg gcagacggcg
    11341 gttgcgcgtc tccgcaaaaa tgcagggcgc gcggttttca gacggcattt gccgttcaag
    11401 gccgtgcggt gtctttacca aatgcccaac cattcgccca cggaatccat ccaatcctta
    11461 ttgcccccgc cgctcctgcc tgcccggcgg tacgcccacg gcgcttgcgg atttttagct
    11521 ttccacaatc ctttgcgttc cctttccgcc tgaatttgag cgtcggcata gtcggcaaaa
    11581 tccgccttat cctgctgttc tttagcataa cttttataat gccacgccgc cccgtcctgc
    11641 acctgcatca ggttcaaatc ggttttgccg gcggatacct gcgccacttc gcgctgatag
    11701 cggtcggttt caaacacacg tacactgact ttcctaccct ccgccgccgc gcgcaggttg
    11761 tcgcgcgaac gtgtaccgta agcctgtttc atctccggtg cgtcgatata cgccatccga
    11821 attttatgtt tcgcgccgtc gccgtcgatg acgtgaaggg tatcgccgtc atagactttg
    11881 gacaccgtgc ctgtgtagct gtggccggat ttcgccgatg cccgtcggcg aacgggcgcg
    11941 tcggaaccca cgtcccctgc cgtgccgagt acgtcgagta cggcaaccgc cgtccgcacc
    12001 gcctcactgt catatcccgt ataacccaac gcgcccaaaa gcgacagggc gacgggaagc
    12061 catttcatga tttttttaat ctgcatattt ttcaaatgcc gatgccgtct gaacatctct
    12121 gaaccggatt tcagacggca tcttaacgtc aggattaccc ttggcaggga tagatgactt
    12181 tcgcaccctc ttccgtcccc aaaatcaaca catcggcggc atcgcgggcg aatatgccgt
    12241 tttcgagtac gccggtgatt ttgttgattt catcttccat cgtcagcggc tgatcgatat
    12301 tcaagccgtg gacatcgacg atttggttgc cgtaaaacgt ggtgtagccg atacgcagtt
    12361 cgggctgtcc gcccatggca agcagtttgc gcgaaacaag ggagcgcgcg ctttcgacgg
    12421 cttccacggg cagtgggaat ttgcccagcc gggaaacata tttgctttcg tccgcaatgc
    12481 agacgaattt ttctgatgcg ctggcgacga ttttttcgtt gaggtgcgcg ccgccgccgc
    12541 ccttaatcat ttgcagggcg tggttcactt cgtccgcacc gtcgatatag accgccaaac
    12601 cggacacttc gttcagggac acgacgggaa tgtcgtaccg ggcaagcagt tcgccggatt
    12661 ttttggaggt tgaaaccgcg cctttgattt ttttaccgct tttgcccaag gcttcgatga
    12721 agaaattgat ggtcgaaccg gtaccgatgc cgatgtattc gttttcgggt acgaattcga
    12781 ctgctttttc ggcggcgatg cgcttgagtt cgtcttgtgt cgtcatattt ttatcctttg
    12841 ggaaaccgta tcaacaaaca gccgccatct taacattttt ttgcacgtcc tgcccgccgc
    12901 gttcaaatcc gtaccagcaa taccgccgcc tgcgcctcta tgccttccat ccgcccgaga
    12961 tagccgagtt tttcgttggt tttgcctttg atattgacgc acgaaatatc gatacccaaa
    13021 tcggcggcga tgttggcacg catttgcgga atgtgcggtg cgagtttggg tttctgtgca
    13081 atcacggtcg tatcgacatt gaccgcctgc caaccctgcg cctgaacgct ttgatacgcc
    13141 gcacgcaaaa ggacgcggct gtccgcatct ttgaactctg cggcggtgtc gggaaaatgg
    13201 ctgccgatgt cgcccaaacc tgccgcaccg agcagcgcgt cggtaacggc gtgcagcagc
    13261 gcgtcggcat cggaatgccc gagcagccct ttttcaaatg ggatttcaac tccgccaagt
    13321 atcagctttc tgccttcgat caattggtgg acatcgtagc cctgtccgat acggatattc
    13381 gtcatcgttt gtgttcctga tgttttgaat tgaatttcag acggcattga gcagcagcct
    13441 gacgatgtat gcgtcctgcg gctgcgtcag tttcaaattg cgcgcgtcgc cctgtatcag
    13501 tagcggacgc acacccagtt tttccacggc ggacgcttca tcggtaatgc cgtccaagtt
    13561 ttccgcagcc aatgcgcggt gcagcaaacc cgcttgaaaa agctgcggcg tttgcgcctg
    13621 ccaaagcccc gaacggtcga cagttgcact gatttgtccg ctttctgcgc gcttgagcgt
    13681 atcggcaacg ggaactgcca aaatcccgcc ttcggcggcg ttgcccgcct gttctatcaa
    13741 ccgcgtcaga gcttcagacg gcaggcagca gcgggcggca tcgtgtacca gaatattgtc
    13801 ggtttccgcc gccaaaccgg tttccaacag ttttgccaca ccgttgcgga cagtttcggc
    13861 gcgggtctgt ccaccgtttt tccacacccg aacctgtgga aatgccgtct gaaccttatc
    13921 ggcaaacgtg tcttcgggcg agacgacaac gacggtcaaa tcgacggcct catgccgttc
    13981 aaaaatccca agtacatgtt ctaaaacggt tttgcttccg atttcgacat attgcttggg
    14041 tttgtccgca ccgaaacgca ccccgatgcc ggcggcggga atcagcgcga tatttttgcg
    14101 tttcatgcgt tttccccgcc gttttcagac ggcacggctt ccttacgcca aatacaggct
    14161 tcgcccaagc cgtcaagata ttcttcgtgc gccgccagtt cggcttcatc cgccttaatc
    14221 actttcagtt tggcaccgag tttcatttcg gcagatgcaa caggtttggc tgtcggttct
    14281 tcctcctcgg tctcccccat caagtcgaat tgccgccgcg tcatcgccag atagacttcg
    14341 cccaaaagtt cgcagtcgat caatgcgccg tgcaggacgc gcttgctgcg gtcgacggaa
    14401 aaacggttgc acagggcatc cagactggct ttctgcccgg ggaacatttc gcgcgccatc
    14461 gccagggtat cggtaacggt gcagccgagt tcctcgacgg tcggcagccc catccggcgg
    14521 aactccatat tgaggaagcc cacatcgaat ttggcattgt ggataatcag ttccgcaccg
    14581 cgcagaaaat cggcaatctg cctgccgacc tctgcaaacg gcggcgcgtt tttctcttcc
    14641 aaaacctgta tcgtcaagcc gtggacgcgt gccgcctctt cgggcatatc gcgctcgggg
    14701 tggacataga ggtgcaggtt tttgtcggtc atttggcggt tgaccatttc cagaccggca
    14761 aactcgacca gacggtcgcc gccgtcggca tacaggccgg tggtttcggt atcgaggatg
    14821 atttggcgtg tcatcatatc ggtgtctttc ttctgtcttc gtaaattgat tattttttaa
    14881 gcaatgtatt tttctgtttt catttcaatg cacaaaccca cttattcaca gtgtgttcac
    14941 aacattgggc aggcggattg tgtattttgg ggacaatttt ttcagacggc attcaaggtt
    15001 ttttcctgat tgccgccgcg cctaaaaacc gcctttcgcg cttaatcaaa aataccgcca
    15061 acggaatatt gcccaaggcg acaatcagat acagcaggga aatgctgtca aacaaaaaca
    15121 gcaataccgc gctcaaaacg gcggcggaaa ccataaagat gccgttaacg atattgttgg
    15181 cggcaacggc gcgggcgcgg aaagtctcgc tgctggcggt ttgcagccag gtatagagcg
    15241 gaacggagaa aaatccgccg aaaaagccga tcagcgtcat caccgccatc acggagtatg
    15301 cccatccttg cgataaaaac caaaaaatgc cgttcagccc ttcaaaacgg tgtccgtgcg
    15361 tcagccacac caaaaccagg ccgcaaaccg tcaaacccaa cgcaccaacc gttacccaag
    15421 ccaacatcag ccgttccctg ccgaacttgg cacacagtac cgaaccggcg gcaataccga
    15481 tggaaaacaa agcaagcatc aggttaaaaa cattatcgtt gccgcccaaa cggatttggg
    15541 taaaggtcgg cagttgcgtg gtataaaccg cgccgacaaa ccaaaaccac gagatgccga
    15601 taatggcggt aaaaacggga ttgtgccgca ccgtttcacg cagcagggat tttgtaccac
    15661 ggacaatatt ccactcgatt tgggtatcgg cagccttggc gggtacggac ggcataaaca
    15721 ggctgccggc cgttcctccg acggcgacca gcaaaaccag tatcccgaca atataaggcg
    15781 gcgcgccggc aaccgccgtt cccaaaatct gaccaaacag gatggcgaca aacgtaccgg
    15841 attcaatcag gctgttgccc atcatcagct cgttgtcgtc gagataatcg ggcagaatgg
    15901 catatttaag cgggccgaac agcgtcgatt gcgcgcccat gcaaaacagg cagagcaaga
    15961 gcagcggggc tgaccggata taaaacccgt atgccgccac cgccataatg atgatttcca
    16021 gcaccttgac ccaacgcgcc aaaacggcct tgtcgaattt gttacccaac tgccccgaca
    16081 gcgcggaaaa caggaaatac ggcaaaataa acagcagtgc gcccaagttc aacatctgtc
    16141 cggcaggcag aacgccgttt tgccccaaac cgtaaaaccc gatcatcaca aacagcgcgg
    16201 ttttgaacac attgtcattg aacgcgccga gaaactgcgt agcgaaaaga ggcccgaaac
    16261 ggcggctttt aaccagtccc aaaccgcctt ttttagcgtc catcgttttc cttctctttt
    16321 tcaatcagtt tacttgtcga atcatcatcc atcaaaatgc ggtgcgccgg tccttccaaa
    16381 tcgtcaaact gtccgttttt gcccgaccac cagaaaaacc agccgatgac aaatgccaaa
    16441 ataatgctga tgggcaccag aataaacata ctttccatca catattccct atcaaatcgt
    16501 tcaaaacaaa agtctgccct gacacggtca gatattcgtt acgcaaagtt ccgacggggg
    16561 cttcgtcaaa aaacagctcg atacggtctt tgaccacgcg ccaatattgg ggaatttccg
    16621 tctgaccgaa cggcgacagg acatggttgc ccgtcccatc ttccaattta acggcaaaac
    16681 gcccgctctg cagccgtgct tccgattcgt cgtcggcaag aaggatgaaa aagcctatat
    16741 gccgtcccga ttggtcatga atactgaaat aatgcataaa ttccccaccc gccttttttc
    16801 agacggcatc aactaaaaac agggcgaatg taccagtttg gacgggaaaa atgcaaagaa
    16861 attctccctc ccccagccga aaacaccggc aaaccgcata tccccctttt ttccgtcaaa
    16921 atacccgact tccgccattt tcacgcaaac gcccgattaa gccaagcaat tgcaaagatt
    16981 ttttgctaga atagcctgct tcttttatca accgtttcag acggctccac tactttcccg
    17041 tcaaggaaag caaagcggat tcggcacgaa tccgattagt atccgtgtcc gattccaatg
    17101 ccgtctgaaa ctttccggag taagaaaatg tcccaaaaat tgatcttggt tttgaactgc
    17161 ggcagctcgt ccctcaaagg cgcggtcctg gataacggca gcggcgaagt cctgctgagc
    17221 tgccttgccg aaaaactcaa cctgcccgat gcctacatta cattcaaagt aaacggcgaa
    17281 aaacacaaag tcgatctgtc cgcacacccc gaccacaccg gcgcggtcga agccctgatg
    17341 gaagaactcg aagcccacgg cctcgacagc cgcatcggcg ccatcggcca ccgcgtcgtc
    17401 agcggcggcg aactgtacaa cgaatccatc ctcgttgacg acgaagtcat cgccggcatc
    17461 gaaaaatgca tcccgctggc tcctctgcac aatcccgccc acctcttggg cctgcgtgcc
    17521 gcgcaaagca ttttcaaagg cctgcccaac gtcgtcgtat tcgatacctc cttccaccaa
    17581 accatgcccg aagtcgccta caaatacgcc gttccgcagg agttgtatga aaaatacggc
    17641 ctgcgccgct acggcgcgca cggtaccagc taccgcttcg ttgccgacga aaccgcgcac
    17701 ttcctcggca aagacaaaaa agacctgcgt atggtcattg cccacttggg caacggcgca
    17761 tccatcaccg ccgtcgccaa cggagaatcg cgcgacacca gtatgggcct gaccccgctg
    17821 gaagggctgg taatgggtac gcgcagcggc gacatcgatc cttccgtatt cggcttcctc
    17881 gccgaaaacg ccaatatgac cattgcccaa atcaccgaca tgctgaacaa aaaatccggc
    17941 ctgctcggca tttccggcct gtccaacgac tgccgcacca ttgaagaaga agccgccaag
    18001 gggcataaag gcgcgaaatt ggccttggat atgtttatct accgccttgc caaatacatc
    18061 ggcagtatgg cggttgccgc aggcggcttg gacgcactgg tctttaccgg cggtatcggc
    18121 gaaaactccg acatcatccg cgaacgcgta atcggctact tgggcttcct cggtctgaac
    18181 atcgaccaag aagccaacct gaaagcccgc ttcggcaacg ccggcgtgat taccactgcc
    18241 gacagcaaag ccgttgccgt ggtcattccg accaacgaag agctgatgat tgcccacgat
    18301 accgcccgtt tgagcggtct gtaaggtttt atcctcacgc gaactgcctc cggaaacgga
    18361 ggcagttttt ttatagtgga ttaaatttaa accggtacag cgttgcctcg ccttgccgta
    18421 ctatttgtac tgtctgcggc ttcgtcgcct tgtcctgatt tttgttaatc cactatatcc
    18481 ggctttccat gcttaaacag cactacctct tttcagacat tgacggttgc agccgcctac
    18541 ctgaacctta atgattgact attttttaat catgttatta ttttccataa aatacatggc
    18601 attaagatgt ttttccacaa aagatacaca caccggcaaa caccggctgt gtttatcttt
    18661 tcttatgcct attttttaat catcgtattt ttatctttta atttcaatac gcaaaccgac
    18721 ttatacacac ggttttcaca tctttggact gcttccgtgt gtatagcgga tattgccgtt
    18781 ttcctgtctg acaaaaatgc cgtctgagaa cttcagacgg catttgaaac atcggaatca
    18841 gcggttttgt tcataccact cgataaactt gtctgctttg acaaaaccca gcagcggctc
    18901 gctgcggctg ccgtcggcgc ggacgacaaa cacgcccggc ggcccgaaca gaccgtattc
    18961 tttcaacaac gcctgatgtt cgggcgtgtt ggcggttacg tcgatctgga aaaagcgttc
    19021 catatcgact gcctgatgca cttccggctg attgagcgtg taaaccgcca tttctttgca
    19081 ggaaatgcac cagtcggcat aaaaatccaa aacgacaggt ttgtcgggat gttctttcaa
    19141 cgccgtatcc atcgctgcct tcagcgcggc agtatcggca aacattttgc cgtgttccga
    19201 aaatttgcct gcttcggccg gcggattgag ggtcaagaaa tggtgcagcg cggtcgtttt
    19261 gccgtttgcg ccctgccagc cgaaccacgc gccgcctatc agcaatatgc tgcccaatgc
    19321 gaatgccaca gctttcggac ggcgtttctg cctgcgtccg ttgaccagca gcataaaggc
    19381 aggaaccagc atcagcagcg tgtacagcgc gacgacgaga taatagggca aatgcggcgt
    19441 ggcgaggtaa acggcgacgg caagcaggat aaagccgaaa gcgtatttga cggcattcat
    19501 ccaatcgccc gccttaggca ggatatgtcc tccgaacgtg ccgatggcaa tcagcggcac
    19561 gccggtgccc aacgccaaag tgtaaagtgc caaaccgccc aaaaccgcat cgcccgtctg
    19621 accgatatag cccaaggcaa acgccagcgg cggggcgacg cacggtccga caatcagtgc
    19681 ggacagaatg cccataataa agacggaaac gattttaccg ccggagagcc tgctgctctg
    19741 gttttggaaa tatgactgta cggcgttggg aagctggatg ttgaacagcc cgaacataga
    19801 cagcgccaag acgaccatca aagccgatgc cgccaacacg acccaagcct gctgcaacca
    19861 tacggtcagc agcgcgcccg tcagtccggc aacaatgccg accagcgtat aagtcagagc
    19921 caaaccctga acataaacga cggacagcac aaacgcccgc gccttgcccg cctttttgtc
    19981 accgaccacg atactggaca caatcggcag caggggatac atacaagcgg taaaactcag
    20041 gcccaaaccg gcgagaaaaa acgccaaaag attggcgttg agcgtatccc aagacagctt
    20101 gaaacggccg tcgccgccct cgtccccctt cgggggcggc aacgccccgc tgccgttttg
    20161 agaggaaggc tgcaaaaaac ggtctttggc ggatgccggt tcgtcggttt gcggatggta
    20221 agtgccgttg ccggaaatat caaactcggt atccacgggc ggatagcaca cgccggcttc
    20281 ggcacagccc tgataggtca aaaccaattt atacggttcg ccgacagcct ttgcataagg
    20341 aaaggcaacc tgcgcctcgt gatggtgaac cgtctgcctg ccgaaaaact cgtcttcctt
    20401 ctcttcgccc ttactgaaag aaggctgtcc caacaaatcc gccggatcgg tcttgccgac
    20461 gattttcgcc tgatacatat agtatccgtc ggcaatcctg aaacggacgt tcacgccgtc
    20521 gtcggcaaca gtaagctccg gcacgaatgc cttttccggc ggcagcagat cgttcgcatc
    20581 cagcgcgaaa gcccgtccgc acaacatcaa aaatacggcg aacaggcaaa tcagtttttt
    20641 cataatcgaa tccgtttcag acaaataatt tgtccgcatt ataaatggta aggttgacgg
    20701 taggatttaa tttatgtaaa acccgccatt atccgaacct atttccataa acatcttatc
    20761 ggacccgcca tgtacgatgt caatacccac gatgtccgcc gctttttcgc ccgcgtgtgg
    20821 cagcagcggc tcaatccgct gcaattgggc gcgttggaac agaaagccct ccgcattgtc
    20881 gaagcccatc ccgaatacca ccgttatctc gaacgcatcg aagaccatct ggacaccgac
    20941 tggctgcccg aaaacggcga aagcaacccc ttcctgcata tgtcgctgca tttgtccgtc
    21001 caagaacagg cgggcataga ccagccgcac ggcatacgcg ccatccacga caccctgtgc
    21061 gccaaacgcg gctggccgga agccgaacac gaaatgatgg aggcactggc ggaaacactg
    21121 tggacggcgc aacgctacgg caccggtttg gatgtcaatt tctacatgac ccgactgcgc
    21181 aaactcatcg gcttgggtgc agaggatcaa gccaggttaa acccgcatga aatcgcctga
    21241 ccataccgac cgcctgcaaa atgccgtctg aagcggaaca acccctttca gacggcattc
    21301 attttccccc aatcatttcc acaacgcgcg tttcaacata atcaaccaat ccttcttatc
    21361 caaaacgggg cgttgtgcaa acacatcgta tcggcacgcg tccagtttct gcaaaatcaa
    21421 ctgcgccccc aacacaatca tacggagttc caaaccgata cgccctttca attcgcgcgc
    21481 caaaggcgaa cccgccttca gcatacggaa tgcacgccgg cactcatacg ccatcagccg
    21541 ctgaaacgcc gcatccgccc gtcctgctgc gatctgttcc tcagaaacac cgaatttcaa
    21601 caaatcgtcc tgcgggatat aaaccctgcc cttttgccaa tccacagcta catcctgcca
    21661 aaaattcacc agttgcaaag ccgtacaaat accgtcgctt tgcgctacgc acaccgcatc
    21721 cgttttcccg tataaagcca gcataatgcg tccgacaggg ttggcggaac gccggcaata
    21781 atcggtcaga tcgccgaaat gcgcgtaccg cgttttaacc acatcctgcg aaaacgccga
    21841 gagcagatca taaaacggct gcaaatccaa accgaacggc acaaccgcct cggcatccaa
    21901 tcgtgcaatc aaaggatgcg ccgaccggcc gcccgatgcc aacacgtcca actcgcgccg
    21961 caaaccctcc aaccccgaca acctggcttc agacggcata ctgccctcgt ccgccatatc
    22021 gtccgccgtc cgtgcaaacg catacaccgc atgaaccggc ttcctcaacc tgcgcggcaa
    22081 aaccagcgaa ccgacgggaa aattctcata atgcccaacc gacatacctt ctccatccat
    22141 caaacaaaat gccgtctgaa acggaacaaa cccttttcag acggcatcag atacctccaa
    22201 gctgccggca atcagtggtg gtaatgaccg tgcgaaccgt ggacatgacc gtgtgcgatt
    22261 tcctcatcgg atgcatcgcg cacgctttca accgtagctt taaagcggat tttcatgcct
    22321 gccaaaggat ggttgccgtc caccaccgcc ttgccgtcgg caacatcggt tacacgatag
    22381 acgacaacat cgccggtttc aggatcgtcg gcttcaaaca tcatgccgac ttcgacttca
    22441 acagggaacg cgcccgcatc ttcaatacgg accaactccg aatcctgctc gccgaacgca
    22501 tcgtcggacg aaagcgccac atcgaccgta tcgccggcat ccttaccgtg caacgcctct
    22561 tccaccaaag ggaaaatgcc gtcgtaaccg ccgtgcagat acgcaatcgg ttcttcggtt
    22621 ttatccaaaa gctgattgtt ggcatcatac atctcataat gcagcgaaac cacggaattt
    22681 tttgcaatag ccatatttgt cctttcagga acagcagatt gattacaggc gcattctaac
    22741 acaaccgccg cgccggccga ttaccgttaa cctgttcata aactgtacgg cacatatttc
    22801 aatgtaaatt tttgttattt tattgcagtg taaatttttt acaacactct caaaaaccat
    22861 tccgacccat ctgccgactt ccccaatctg ccttaataaa tcatacaaga tactgaatta
    22921 tattaatctc tataatattt atccctatcg gatttttaac agcaaaaccg ttttacagga
    22981 tttatcaatc cgcccgccag aaaacttttc attcaaacct ttttcccatc tgtacgacat
    23041 tgcaatccct tattccatgg tgcataatta cgcaaattca gcgatgaatt tccaacccgg
    23101 tttgtagtat ggtcgaaaaa gacctatttg tttcaataat ttaaattgat tttaaaggtt
    23161 actaaaatga aaaaatccct gtttgccgct gctttgttgt ctttggcttt ggctgcctgc
    23221 ggcggagaaa aagccgctga agctcctgct gctgaggctt cctctaccga ggcacctgct
    23281 gccgaagcac ctgccgccga agcacctgcc gccgaagc
//
LOCUS       DAWTQS010000028        23086 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.028, whole genome shotgun sequence.
ACCESSION   DAWTQS010000028 DAWTQS010000000
VERSION     DAWTQS010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23086
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.028"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(86..424)
                     /locus_tag="WNA25_001093"
     CDS             complement(86..424)
                     /locus_tag="WNA25_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFA9823858.1"
                     /translation="MKKIEAIVKPFKLDDVREALTEIGITGMTVSEVKGFGRQKGHTE
                     IYRGAEYAVDFLPKVKIELVLADDAVERAIDVIVEVARSGKIGDGKIFVLPVEEAIRI
                     RTGERSDAAV"
     gene            575..4531
                     /gene="purL"
                     /locus_tag="WNA25_001094"
     CDS             575..4531
                     /gene="purL"
                     /locus_tag="WNA25_001094"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFA9823859.1"
                     /translation="MSVVLPLRGVTALSDFRVEKLLQKAAALGLPEVKLSSEFWYFAG
                     SEKALDAATVEKLQALLAAQSVEQTPEAREGLHLFLVTPRLGTISPWASKATNIAENC
                     GLAGIERIERGMAVWLEGALTDEQKQQWAALLHDRMTESVLPDFQTASKLFHHLKSET
                     FSTVDVLGGGKEALVKANTETGLALSADEIDYLVENYQALQRNPSDVELMMFAQANSE
                     HCRHKIFNADFILNGEKQPKSLFGMIRDTHNAHPEGTVVAYKDNSSVIKGAKIERFYP
                     NAAENQGYRFHEEDTHIIMKVETHNHPTAIAPFAGAATGAGGEIRDEGATGKGSRPKA
                     GLTGFTVSNLNIPDLKQPWEQDYGKPEHISSPLDIMIEGPIGGAAFNNEFGRPNLLGY
                     FRTFEEKFDGQVRGYHKPIMIAGGLGSIQAQQTHKDEIPEGALLIQLGGPGMLIGLGG
                     GAASSMDTGTNDASLDFNSVQRGNPEIERRAQEVIDRCWQLGDQNPIISIHDVGAGGL
                     SNAFPELVNDAGRGAVFELREVPLEEHGLTPLQIWCNESQERYVLSILEKDLDTFRAI
                     CERERCPFAVVGTATDDGHLKVRDDLFSNNPVDLPLNVLLGKPPKTTRTDKTVTPSKK
                     PFHAGDIDITEAAYRVLRLPAVAAKNFLITIGDRSVGGMTHRDQMVGKYQTPVADCAV
                     TMMGFNTYRGEAMSMGEKPAVALFDAPASGRMCVGEAITNIAAVNIGDIGNIKLSANW
                     MAACGNEGEDEKLYRTVEAVSKACQALDLSIPVGKDSLSMKTVWQDGEEKKSVVSPLS
                     LIISAFAPVKDVRKTVTPELKNVEGSVLLFIDLGFGKARMGGSAFGQVYNNMSGDAPD
                     LDDAGRLKAFYSVIQQLVAEDKLLAYHDRSDGGLFATLAEMAFAARCGISADIDCLMD
                     KFLPIHLPDFQGDPAEDLSDELYNHAAIKILFNEELGAVIQIRQKDRDYVDAAFETAG
                     LTDAVSRIGSPDFDNEFISFFGYGYFLEQNRADLQRAWQETSHAIQRLRDNPACADSE
                     FALIGDNERSALFADVKFDVNEDIAAPFINSGAKPKIAILREQGVNGQIEMAAAFTRA
                     GFDAYDVHMSDLMAGRFRLADFKMLAACGGFSYGDVLGAGEGWAKSILFHPALRDQFA
                     AFFADPNTLTLGVCNGCQMVSNLAEIIPGAETWPKFKRNLSEQFEARLNMVHVPKSAS
                     LILNEMQDSSLPVVVSHGEGRADFALHGGNISADLGIALQYVDGQNQVTQTYPLNPNG
                     SPQGIAGITNADGRVTIMMPHPERVYRAAQMSRKPEGWTELSGWYRLFAGARKALG"
     gene            complement(4602..5813)
                     /locus_tag="WNA25_001095"
     CDS             complement(4602..5813)
                     /locus_tag="WNA25_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007241529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase family
                     protein"
                     /protein_id="HFA9823860.1"
                     /translation="MLFTPFTFQNGKTAKNRIFKSAMEEQLAQNDQPSEKLVRLYGAW
                     AEGGAGVLVTGNVMVAESGKGSINDVLISDDRALEMLKKWAKARMQNDTLLIMQINHA
                     GKQSPAVVNKTPLAPSAVPLVGMNGFINPPRELSADEINGLIQQFVQTAKIAEQAGFS
                     GVQIYAVHGYLISQFLSPHHNRRQDQWGGSLENRMRFLLETYTAIRAAAGKDFLVGVK
                     LNSADFQKGGFDESESVQVVQKLSEMGIDFIEVSGGNYESPQMLAAKDSTRKREAFFI
                     DYAEKARAASQAPLIITGGFRSQTAMEDALSSGHLDLVGIARPFALVPDLANKMQNRT
                     YQTVQADRIQTGVAFVDKKAGAMLEMNWYMTQMDLIGQGKQSNPKLSAWKVLLKTLRE
                     NGKAGLSTGRA"
     gene            complement(5887..6198)
                     /locus_tag="WNA25_001096"
     CDS             complement(5887..6198)
                     /locus_tag="WNA25_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFA9823861.1"
                     /translation="MPQQTMNLMRECIPIFTVLSDENRHQILHVLWKHGRMNVNELTE
                     HLHLSRPAVSHHLKIMLQAGAVAVEQVGKERFYSIAMADAVARLKQLADLMAQNCPLS
                     K"
     gene            6430..7182
                     /gene="gloB"
                     /locus_tag="WNA25_001097"
     CDS             6430..7182
                     /gene="gloB"
                     /locus_tag="WNA25_001097"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFA9823862.1"
                     /translation="MKITPVKALTDNYIWMIQHGNHAVCVDPSEPSPVLEFLVRNRLM
                     LAQTWVTHPHPDHEGGAAALWRGYMESPVYGESDIEAATHTVTAGTRFTFGNGQVTVW
                     ATPGHTDRHTSYLLETSDGIHVFCGDTLFSAGCGRVFTGTVEQLYDSFQRFNQLPEGT
                     LFYPAHEYTAANLRFAAHIEPDNADIQTALKAAERTPTLPVTLAHERRVNPFLRTEIP
                     AVRQRAEALVGKTLNSGLEVFAALRELKNAYR"
     gene            7416..8870
                     /gene="mgtE"
                     /locus_tag="WNA25_001098"
     CDS             7416..8870
                     /gene="mgtE"
                     /locus_tag="WNA25_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFA9823863.1"
                     /translation="MSIEHTPPTHDGETGQNHAERPSADFDRVHSLCEILEPAFEQIE
                     NGTPLEDAPLRDKLTELTVLLAELHPADVADVLESLPPRERNIVWLLVKPEDDGEVLL
                     EVSDAVRETLIESMDKDELLAAVDDLDADELAELADDLPHQVVYEALQTRDEEERAQV
                     KAAMSYEDNQVGAIMDFELVCIRADVACEVVLRYLRRFDSLPDHTDKIFVVDENDVLQ
                     GVLPIRKLLVADPEDLVENVMAKDVVRFRAEDDVEEAAQAFERYDLVTAPVVDENKKL
                     IGRITIDEMVDVIREESEADMLNMAGLQEEEDLFAPVLDSVKNRWMWLAVNLCTAFLA
                     SRVIGAFEGSIEKIVALAALMPIVAGIGGNSGNQTITMIVRAMAMGQLTDMQAGRLLK
                     KEVGVALVNGIIWGTVMGAVSWLLYGSLGIGLVMVAAMTLNLLLAATVGVLIPVVMEK
                     FGRDPALGSSVLITAVTDSGGFLIFLSLATLFLL"
     gene            complement(8956..9864)
                     /gene="hslO"
                     /locus_tag="WNA25_001099"
     CDS             complement(8956..9864)
                     /gene="hslO"
                     /locus_tag="WNA25_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFA9823864.1"
                     /translation="MNQTAINRADARTRFIFDDMPVRGLHVRLENVWQHIVKQKNYPA
                     AIRCALGELLAAGVLLSGNLKNEGTLIVQVQGQGKLKMLVAEATSDRTVRATARWDET
                     AEIADDESLGDLLGGNGVFVLTLQPKDGEPWQGVVPLEGGSIAQMLVNYMKRSEQLDT
                     HIALSASDEAAGGLLVQRLPEEVLDEEAWEHVSTLARTLTAEELAELDAQHVLYRLFH
                     ETPPRVFEPETFESSCTCSRGKVSDMLLMLGGEEVGGVVAEQGSIEVDCDFCHSKYVF
                     DETDVNALFGEDVVGVAKGLPRHTVQ"
     gene            10109..10690
                     /locus_tag="WNA25_001100"
     CDS             10109..10690
                     /locus_tag="WNA25_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFA9823865.1"
                     /translation="MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAED
                     EQPVLPVNRAPARRAGNADELIGSAMGLLGIAYRYGGTSVSTGFDCSGFMQHIFKRAM
                     GINLPRTSAEQARMGAPVARSELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTG
                     KNIEITSLSHKYWSGKYAFARRIKKNDPSRFLN"
     gene            10709..10927
                     /locus_tag="WNA25_001101"
     CDS             10709..10927
                     /locus_tag="WNA25_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823866.1"
                     /translation="MSMPEMPKWYGDDGQIVSCTEKVKVMSENMAELYQTAQDAFEDA
                     LLMGCGERQLRAYLLALIEGLENPYRKV"
     gene            11072..11416
                     /locus_tag="WNA25_001102"
     CDS             11072..11416
                     /locus_tag="WNA25_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFA9823867.1"
                     /translation="MNIIRALLIILGCLAAGETAVFLAGIKLPGSIVGMGVLFALLQA
                     GWVKTSWLQQLTDALMANLTLFLVPPCVAVISYLDLIADDWFSILVSASASTLCVLLV
                     TGKVHRWIRSII"
     gene            11416..12108
                     /locus_tag="WNA25_001103"
     CDS             11416..12108
                     /locus_tag="WNA25_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFA9823868.1"
                     /translation="MSEILRQPGILLFLTLAVYALAIIVRTRTGNIFCNPVLVSTIVL
                     IAYLKILGIDYAVYHNAAQFIDFRLKPAVVVLAVPLYQNRRKIFNQWLPVIVSQLAGS
                     VTGIVTGMYFAKWLGAEREVVLPLASKSVTNPIAIEITSSIGGIPAITAATVIIAGLV
                     GQIAGYKMLKNTVVMPSSVGMSLGTASHAMGIAASLERSRRMAAYAGLGLTFNGVLTA
                     LIAPLLIPVLGF"
     gene            12175..13395
                     /gene="argJ"
                     /locus_tag="WNA25_001104"
     CDS             12175..13395
                     /gene="argJ"
                     /locus_tag="WNA25_001104"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HFA9823869.1"
                     /translation="MAVNLTEKTAEQLPDIDGIALYTAQAGVKKPGHTDLTLIAVAAG
                     STVGAVFTTNRFCAAPVHIAKSHLFDEDGVRALVINTGNANAGTGAQGRIDALAVCAA
                     AARQIGCKPNQVLPFSTGVILEPLPADKIIAALPKMQPAFWNEAARAIMTTDTVPKAA
                     SREGKVGDQHTVRATGIAKGSGMIHPNMATMLGFIATDAKVSQPVLQLMTQEIADETF
                     NTITVDGDTSTNDSFVIIATGKNSQSEIDNIADPRYAQLKELLCSLALELAQAIVRDG
                     EGATKFITVRVENAKTCDEARQAAYAAARSPLVKTAFFASDPNLGRLLAAIGYADVAD
                     LDTDLVEMYLDDILVAEHGGRAASYTEAQGQAVMSKDEITVRIKLHRGQAAATVYTCD
                     LSHGYVSINADYRS"
     gene            13455..14852
                     /locus_tag="WNA25_001105"
     CDS             13455..14852
                     /locus_tag="WNA25_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HFA9823870.1"
                     /translation="MTSTFPRRLARKIRQTRRLSRKSIAFLFLLAGSALVALTALFFA
                     HLADFALELNAKLVQQYPWFAWIALPPGLPVIVWLTRKFAPFTAGSGIPQVIASLSLP
                     YGAQKTRLIRLGQTLLKIPLTFLGMLFGASIGREGPSVQVGAAVMGAWGAWCKKHGLA
                     FKGMQENDLMAAGAAGGLAAAFNAPLAGVIFAIEELGRGIMLRWERQILLGVLAAGFI
                     QVAIQGNNPYFSGFNGGVLENILMWIVAAGLVCGAAGGIFARMLHRGAAAFAPRKIRG
                     FIRNRPLLLAALMGLLLALLGTFYQGKTYGTGYHEAAQALHGIYEAPVGLAAAKWLAT
                     VFSYWAGIPGGIFTPSLTIGAVLGEHIAAIADISQGANIIVLICMAAFLAGATQSPIT
                     SAVVVMEMTGGQSLLFWMLIACIFASQVSPRPFYHASGMRFRRRVLQETAAQTGNAPA
                     RPQAANSKTGMPSEN"
     gene            15040..16431
                     /gene="hrpA"
                     /locus_tag="WNA25_001106"
     CDS             15040..16431
                     /gene="hrpA"
                     /locus_tag="WNA25_001106"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFA9823871.1"
                     /translation="MFPDFSQTLSKDRHFLQSAFKNPNKYGGLSKIEEKYRKSHEIFL
                     KRLAALPKPEFDNTLPVHEKLEEIKKTVAENQVTIICGETGSGKTTQLPKICLELGRG
                     AAGLIGHTQPRRLAARSVAERIAEELKSEIGSAVGYKVRFTDHTSRDACVKLMTDGIL
                     LAETQTDRYLAAYDTIIIDEAHERSLNIDFLLGYLKQLLPRRPDLKVIITSATIDAER
                     FSRHFNGAPVLEVSGRTYPVEILYRPLTGKDEDDAEVELTDAIVDAADELARYGEGDI
                     LVFLPGEREIREAAEALRKSTLRRNDEILPLFARLSHAEQHKIFHPSGAKCRIVLATN
                     VAETSLTVPGIKYVIDTGLARVKRYSARAKVEQLHVEKISQAAARQRSGRCGRVSAGV
                     CIRLFSEEDFNSRPEFTDPEIVRSNLAAVILRMASLNLGDVAAFPFLEMPDSRYINDG
                     FQVLLELGAVEVV"
     gene            16499..18001
                     /locus_tag="WNA25_001107"
     CDS             16499..18001
                     /locus_tag="WNA25_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HFA9823872.1"
                     /translation="MNIKEFMSNYTNHPVLFIGTGMSLRYLDNSYTWDGLLSKIAIDL
                     FGDDREYLNIKSRYCEDGRFQYEEIAEELQSKFDKVLENDPDGRFKEINDKFFENMRA
                     GNTLSRFKIYISTLLSQLNYKDNSNTELSELKKARKNVGSIITTNYDKLAQDIFEFNP
                     LIGNDILLSNPYGSVYKIHGCVDDPSKIIITKKDYEKFKEKYELIRAQLLSLFIHNPI
                     IFLGYNVGDENIKEILKTIFTYVEPNSPSANKIRRNFLLVEYEPESNNEDIVEHDIDI
                     TGFSTIRINKIKTDNFSQIYKALAELTLPISAMDVRKFQSIAKEIYTGGNIKVSFTED
                     MDNLNNSDKVVAIGSTKTISYNFQTTSEMMSNYFKIIEEENSQLLKLIDKHSIASTQY
                     FPIYGFSRICSDIHKEAVLKRQQKEKLDHFIEEINRRCKNNHSSIQSILDDENISDTY
                     KNDAIAWGIWNNQLSEDEVENYLKNFVNKKNTHYKRLLCMFDYKKYADTV"
     gene            18228..19806
                     /gene="lpt3"
                     /locus_tag="WNA25_001108"
                     /pseudo
     CDS             18228..19806
                     /gene="lpt3"
                     /locus_tag="WNA25_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223145.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase"
     gene            19872..>23086
                     /locus_tag="WNA25_001109"
     CDS             19872..>23086
                     /locus_tag="WNA25_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HFA9823873.1"
                     /translation="MQNMKNQVRGSGMDARSNPANVSDGLQNSSGHIGTNTRYRLTKL
                     GEQMARLPIDPKIARILLAAKKHDCMAEILVIASALSIQDPRERPLEARDAAAKAHER
                     FTDKQSDFLTYLNIWDSFQRERDKGLSNKQLVQWCRQYFLSHLRMREWRELHHQLAQT
                     AIEMGLTTKEAAFRRPPEVKQLTSSENAGDQDLSAKLKQKQLDKKQHRTQIRATKEAG
                     YEQIHRALLTGLIANVGMKSPDSNDYTGARGSRFHLFPASALFKAKPKWVMAAELVET
                     TRLYARDVAVIQPEWIEQEAPHLVRYHYFEPHWEQKRGEVVASERVTLYGLAVLPRRP
                     VSYGKVAPEEAREIFIRSALVAQEYDLKADFFVHNKKLIKEITELEHKSRKQDVLVDD
                     EALFAFYHERLPDFYMADSVSEGLCPANPQQTTPSPVGEGWGEGKTVAAQTNFSATSA
                     SPLPNPLPQEREQSASVSTVSGSLKTMSCEARLNFCEAKTKTESSLHSQRLSENYTPP
                     FSDGLRPANPQQTTPSPVGEGWGEGKTVAAQTNFSATAANPLPQEREQSASALTVSDD
                     PKPKKQPASQKGRLKPLPLADIRTFEAWLKTAERDNPRLLFLSRDDLMQHAAAHITEE
                     QFPKHWQTADGKFKLSYRFEPHHPLDGVTLTLPLTVLNRISPAALEWLVPGMIREKIQ
                     LQIKALPKQIRRICVPVPEFITQFLSQNPDRNAPILPQLAQAIAKTAGDIRILEQINQ
                     DEWAAFRLPEHCYFNLRIIDDGGQELAMGRDLIQIQQQLGKAATTTFRDNTQEFERDN
                     VTAWDIGILPESIKFARGKQQLTGYLGLQKEKDGRIALRLFDTSAAAGHAHRLGVIEL
                     MKLQLKEQVKDLNKGIQGFTQAAMLLKHINADTLRDDLTQAVCDRAFIGEDKLPRNEK
                     AFKEQIKRARSRLPAVKEALSRYLQETAAAYAELNGKLGKHPLTHLLRLRLQTLLAPG
                     FATRTPWAQWPRLPIYLKAMTLRLEKYSSNPARDAAREADIQELEQMWQEKTDGLAKQ
                     GQPVSDGLAAFKWMIEELRVSLFAQELKTPYPVSVKRLLKM"
BASE COUNT         5651 a         6734 c         5726 g         4975 t
ORIGIN      
        1 agggtaacat atctgctaat ctggtacaga cccaaaaaca aatgccgtct gaaagccttt
       61 cagacggcat ttccattcca aacgctcaaa ccgccgcgtc cgaacgttcg cccgtgcgga
      121 tacggattgc ctcctcgacc ggcagcacaa aaatcttgcc gtcgccgatt ttgcccgaac
      181 gcgccacctc gacaatcacg tcaatcgcgc gttccacggc atcatccgcc aacaccaact
      241 cgattttgac cttgggcagg aaatcgacgg cgtattccgc accgcgatag atttccgtat
      301 gccccttctg cctgccgaac cctttgacct cgctgacggt catgcccgta atgccgattt
      361 ccgtcaacgc ctcgcgcacg tcgtcgagct tgaacggttt gacaatcgcc tcgatttttt
      421 tcataaaatt tcctttgaac aaacaataca aacacatccg aaaaacggga acccccgtca
      481 gattgtcaac attttaaacc aaaataccca agcaatacag cccccgttgc gcatataatg
      541 acagcttttc caaccgcatt tgagagccga atccatgtct gttgttttgc ccttgcgcgg
      601 cgttaccgcc ctttccgatt tccgtgtcga aaaactcttg caaaaagccg ccgcactcgg
      661 cctgcccgaa gtcaaattaa gcagcgaatt ttggtatttc gccggcagcg agaaagcact
      721 tgatgccgcg actgtcgaaa aactgcaagc cttgttggcg gcgcaaagcg ttgaacaaac
      781 gcccgaagcg cgcgagggtt tgcatttgtt tttggtaacg ccccgtttgg gtacgatttc
      841 gccgtgggct tccaaggcga ccaatatcgc ggaaaactgc ggtttggcag gcatcgaacg
      901 catcgagcgc ggtatggcgg tgtggctgga aggtgcgctt accgatgaac agaaacagca
      961 atgggcggcg ttgctgcacg accgcatgac cgaatccgtg ctgcccgatt ttcagacggc
     1021 atccaaatta ttccaccatc tcaaatccga aaccttctcc accgtcgatg ttttgggcgg
     1081 cggtaaagag gctttggtca aagccaatac cgaaacgggt ttggcactct ccgccgacga
     1141 aatcgattat ctggttgaaa actatcaggc tttgcagcgc aatccgtccg atgtggagct
     1201 gatgatgttc gcgcaggcaa acagcgaaca ctgccgccac aaaatcttca acgccgattt
     1261 catcctcaac ggcgaaaaac aaccgaaatc cctgttcggc atgatacgcg acacgcacaa
     1321 cgcgcatccc gaaggtacgg tcgtcgccta taaagacaat tcgtccgtga tcaaaggcgc
     1381 gaaaatcgag cgtttctatc cgaatgcagc ggaaaaccaa ggctaccgtt tccacgagga
     1441 agacacccac atcatcatga aagtggaaac gcacaaccac ccgaccgcca tcgcgccgtt
     1501 tgcgggcgcg gcaacaggcg cgggcggcga aatccgcgac gaaggtgcga cgggcaaagg
     1561 ttcgcgtccg aaagcgggct tgaccggctt taccgtgtcc aacctcaaca tccccgacct
     1621 caaacagccg tgggaacaag actacggcaa gccggaacac atttcctcgc cgttggacat
     1681 catgattgaa ggcccgattg gcggtgcggc gttcaacaac gaattcggcc gcccgaacct
     1741 cttgggctac ttccgcacct ttgaagaaaa atttgacggt caggttcgcg gctaccacaa
     1801 accgattatg attgccggcg gtttgggcag cattcaggcg cagcagacgc acaaagacga
     1861 aatccccgaa ggcgcactgc tgatccaact gggcggcccg ggcatgctca tcggcttggg
     1921 cggcggtgcg gcttcttcga tggataccgg tacaaatgat gcgtctttgg acttcaactc
     1981 cgtacaacgc ggcaaccctg aaatcgaacg ccgcgcgcag gaagtcatcg accgctgctg
     2041 gcagctcggc gaccaaaacc cgattatctc catccacgac gtgggcgcgg gcggcttgtc
     2101 caacgctttc cctgaattgg tcaacgatgc cggacgcggc gcggtattcg agctgcgcga
     2161 agtgccgttg gaagaacacg gtttaacgcc tttgcaaatc tggtgtaacg aatcgcaaga
     2221 gcgttatgtc ttgtcgattt tggaaaaaga tttggacacc ttccgcgcca tctgcgaacg
     2281 cgaacgctgc ccgtttgccg tagtcggcac ggcgaccgac gacggtcatt tgaaagtgcg
     2341 cgacgatttg ttctccaaca accccgtcga tttgccgctg aacgtcttgc tcggcaaacc
     2401 gcccaaaacc acgcgtaccg acaaaacggt tacgccgtcc aaaaaaccgt ttcacgcggg
     2461 cgatatcgac atcaccgaag ccgcctaccg cgttttgcgc ctgcctgccg tagccgccaa
     2521 aaacttcctg attaccatcg gcgaccgcag cgtcggcggc atgacccacc gcgaccaaat
     2581 ggtcggcaaa taccaaaccc ccgtagccga ctgcgccgtt accatgatgg gcttcaacac
     2641 ctatcgcggc gaagcgatgt ctatgggcga aaaaccggcc gtcgccctgt ttgacgcgcc
     2701 cgcctcgggc agaatgtgcg tcggcgaagc catcaccaac atcgcggcag tcaacatcgg
     2761 cgacatcggc aacatcaaac tttccgccaa ctggatggcg gcgtgcggca acgaaggcga
     2821 agacgaaaaa ctctaccgca ccgtcgaagc cgtttctaaa gcctgccagg cattggattt
     2881 gagcattccc gtaggtaaag acagcctgtc gatgaaaacc gtgtggcagg acggcgaaga
     2941 gaaaaaatcc gtggtttcgc cgttgagcct gattatttcc gcgttcgcgc ctgttaaaga
     3001 cgtgcgaaaa accgttacgc ccgaattgaa aaacgtcgaa ggcagcgtat tgctgtttat
     3061 cgatttgggc ttcggcaaag cgcgcatggg cggctcggcg ttcggtcagg tgtacaacaa
     3121 catgagcggc gacgcgcccg atttggacga tgcaggccgt ctgaaagcgt tttacagcgt
     3181 aattcaacag cttgtcgccg aagacaaact tttggcgtat cacgaccgca gcgacggcgg
     3241 tttgtttgcg acgctggcgg aaatggcgtt tgcggcacgg tgcggtatta gtgccgatat
     3301 agattgcctg atggataaat tcctgccgat tcatctcccg gattttcaag gcgaccctgc
     3361 cgaagactta tctgacgaac tttataatca tgccgccatt aaaatcttat tcaatgaaga
     3421 attaggcgcg gttatccaaa tccgccaaaa agatagggat tatgttgatg cggcatttga
     3481 aacggccggc ttaaccgatg cggtcagccg gattggctct cctgattttg acaatgagtt
     3541 tatttctttc tttggttacg gttatttctt ggaacaaaac cgcgccgacc tgcaacgcgc
     3601 gtggcaggaa accagccacg ccatccaacg cctgcgcgac aaccctgcct gcgccgacag
     3661 cgagttcgcc ctgattggcg acaacgaacg cagcgcattg tttgccgacg tgaagttcga
     3721 cgtgaacgaa gacatcgccg cgccgtttat caacagtggc gcgaaaccta aaatcgccat
     3781 cctgcgcgaa cagggcgtaa acgggcaaat cgaaatggcc gccgccttca cccgcgccgg
     3841 attcgatgcc tacgacgtgc atatgtccga cctgatggct ggccgcttcc gccttgccga
     3901 cttcaaaatg ctggcggcgt gcggcggctt cagctacggc gacgtactcg gcgcgggtga
     3961 aggctgggcg aaatccatcc tgttccaccc tgccttgcgc gaccaattcg ccgccttctt
     4021 tgccgatccg aacacgctga cattgggcgt gtgcaacggc tgccaaatgg tcagcaacct
     4081 tgccgaaatt atcccgggcg cagaaacatg gccgaagttc aaacgcaacc tgagcgagca
     4141 gtttgaagcg cgcctgaata tggttcacgt ccccaaatca gcgtcgctga ttttgaacga
     4201 aatgcaagac tccagcctgc ctgtcgtggt cagccacggc gaaggccgcg ccgacttcgc
     4261 gcttcacggc ggcaatattt ccgccgattt gggcatcgcg ctgcaatatg tggacggtca
     4321 aaaccaggtt acccaaacct atccgctcaa ccccaacggc tcgccgcagg gcatcgccgg
     4381 cattaccaac gccgacggcc gcgttaccat tatgatgccc caccccgaac gcgtgtaccg
     4441 tgccgcacaa atgagccgga aaccggaagg ctggacggaa ctgtccggct ggtaccgcct
     4501 ctttgccggc gcacgtaaag ccttgggcta aaccgtctgc ccaaacaaat gccgtctgaa
     4561 aaaggttttg aaacccgttt cagacggcat tttgaccatt ctcaagccct gccggtactc
     4621 aatcccgctt taccgttttc ccgcaaagtt ttcagcaata ctttccacgc cgacaattta
     4681 gggttggact gttttccttg tccgatcaaa tccatttgcg tcatatacca gttcatttct
     4741 aacattgcac ccgctttttt atcaacaaat gctacgccag tttggatgcg gtctgcttgt
     4801 acggtttggt aagttctgtt ttgcattttg ttcgccaaat cagggactaa ggcaaacggg
     4861 cgggctatgc cgaccaaatc caaatgaccg ctggataagg catcttccat ggcggtttgc
     4921 gaacggaatc cgccggtgat aatcagcggg gcttggctgg ctgcacgggc tttttcggcg
     4981 taatcgatga agaaggcttc gcgtttgcgg gtgctgtctt tggcggcgag catttgcggg
     5041 ctttcgtagt tgccaccgga aacttcaata aaatcaatgc ccatttccga cagtttttgc
     5101 accacctgca ccgattcgct ttcgtcaaat ccgccttttt ggaaatctgc cgaattgagt
     5161 tttacgccca ccaagaaatc tttgcccgcg gcagcgcgaa tagcggtgta ggtttccaaa
     5221 aggaagcgca tacggttttc caaactgccg ccccattggt cttggcggcg gttgtggtgc
     5281 ggcgagagga attggctgat gagataaccg tgtacggcgt aaatttgtac gcctgaaaag
     5341 cctgcctgtt cggcaatttt ggcagtttgt acgaattgct gaatcagccc gttgatttca
     5401 tcggcactca attcgcgcgg cggattgata aagccattca tgcccaccag cggcacggcg
     5461 ctcggtgcaa gcggtgtttt attgaccacc gcaggcgact gtttgcccgc atgattgatt
     5521 tgcatgatga gcagcgtgtc gttttgcatg cgggctttcg cccatttttt cagcatttca
     5581 agggcgcggt cgtctgaaat caacacatcg tttatcgaac ctttgccgct ttcagccacc
     5641 atcacattgc ccgtcaccaa aacgcctgcg ccgccttctg cccaagcgcc gtacaggcgg
     5701 acaagttttt cagacggctg gtcgttttgg gcaagttgct cttccatggc ggatttgaag
     5761 atacggtttt tggcggtttt gccgttttga aaagtgaatg gcgtgaataa catgatcgtt
     5821 cctttaagtt gataagttta aaaatgtaaa cgtatttggg taaaaataaa gggccaacgg
     5881 ataaccctat tttgaaagcg ggcaattttg agccatcaaa tcggcaagct gtttcaatct
     5941 cgccaccgca tccgccatgg caatactgta aaaccgctct ttgccgacct gctccaccgc
     6001 caccgctccg gcttgcagca tgatttttaa atggtgcgac acagcagggc gcgacagatg
     6061 cagatgctcg gtcagctcat tcacattcat cctaccgtgt ttccacaata catgcaggat
     6121 ctgatggcgg ttttcatcgc tcaatacggt aaaaatgggt atgcactcgc gcattaaatt
     6181 catggtttgt tgcggcatat tcgatctatt gagtttatat atttaaaaat tcgaacgtat
     6241 tttagaggga aggtcggcag ttatcaaagg cagtttggtt tgcagtttgg caatatattg
     6301 ttgctgaaag gtttttataa gaaaaaatgc cgcctgaaac agcatgatgg cgtttgctag
     6361 ggttccgctg tatattaacg cctttcggat acacctttca gacggcatcc gaaccatcaa
     6421 ggaacactca tgaaaattac ccccgtcaaa gccctgaccg acaactacat ctggatgata
     6481 cagcacggca accatgccgt ctgcgtcgat ccttccgaac cctcgcccgt cttggaattc
     6541 ctcgtacgca accgactcat gctcgcccaa acctgggtaa cgcaccccca tcccgaccac
     6601 gaaggcggtg cggcggcact ctggcgcggc tacatggaat cgcccgttta cggcgaatcc
     6661 gacatcgaag ccgccaccca caccgtaacc gccggcacac gattcacctt cggcaacggt
     6721 caggttaccg tttgggcaac acccggccac acagaccgcc acaccagcta ccttctcgaa
     6781 acttcagacg gcatacacgt tttctgcggc gacacccttt tttccgccgg ctgcggacgc
     6841 gtgtttaccg gcacggtcga acagctttac gacagcttcc aacggttcaa ccaattaccc
     6901 gaaggcaccc tgttctatcc ggcacacgaa tacaccgccg ccaacctgcg tttcgccgcc
     6961 catatcgagc cggacaacgc cgacattcag acggcattga aggcggcgga acgcacgccc
     7021 accctgcccg ttaccctcgc gcacgaacgc cgcgtcaacc cgttcttacg gacagaaatc
     7081 cccgccgtcc gccaacgtgc cgaagccctg gtcggcaaaa cgctgaacag cggtttggaa
     7141 gtattcgccg ccctgcgcga actgaaaaac gcctaccgct gacctgccct ccgaaaaatg
     7201 ccgtctgaaa cccgcgtttc agacggcatt tgcgttaaaa atagtaaacc gtttcaaaag
     7261 ggagtagaat agtgctgttt ccaatcccgt gcctgatttt tcgggctttt attatggacc
     7321 ttcccagttc gtttttactg aacacccctt ccgattccaa acgacaataa ccatcccgcc
     7381 ggaatgcctc cccgcacacg gcgggcggag catttatgag catcgaacac actcctccga
     7441 cacacgacgg cgaaaccggt caaaaccatg ccgaacgccc ttccgccgat ttcgaccgtg
     7501 tccactccct ctgcgaaatc ctcgaacctg cttttgaaca aatcgaaaac ggcacaccgc
     7561 tcgaagatgc gccgctgcgc gacaaactga ccgagctgac cgtcctcttg gccgagttgc
     7621 accctgccga cgtggccgac gtcttggagt ccctgccgcc gcgcgaacgc aatatcgttt
     7681 ggcttctggt caaacccgaa gacgacggcg aagtattgct ggaagtatcc gacgcggtgc
     7741 gagaaacgct gatcgagtca atggacaagg acgaattgtt ggcggcggtc gatgatttgg
     7801 atgcggacga attggcggag ctggcagacg atttgccgca ccaagtggtt tatgaagcct
     7861 tacagacgcg cgatgaggaa gagcgcgccc aagtcaaggc ggcaatgtcg tatgaagaca
     7921 accaagtcgg tgcgattatg gacttcgagt tggtctgcat ccgcgccgat gtcgcctgcg
     7981 aagtggtgct gcgttatctg cgccgcttcg acagcctgcc cgaccatacc gacaagattt
     8041 ttgtcgtcga tgaaaacgac gtgctgcagg gcgtgctgcc catccgcaaa cttttggtcg
     8101 ccgatcccga agatttggtg gaaaacgtga tggcgaaaga tgtcgtgcgt ttccgcgccg
     8161 aggacgacgt ggaagaagcg gcgcaggcgt ttgaacgcta cgacttggtt accgcgcccg
     8221 tcgttgatga aaacaaaaag ctcatcggca ggattaccat cgacgagatg gtggacgtga
     8281 tccgcgaaga atccgaagcg gatatgttga acatggcagg tttgcaggaa gaggaagacc
     8341 tgttcgcccc cgtcttggat tccgtcaaaa accgctggat gtggctcgcc gtcaacctct
     8401 gcaccgcctt cctcgccagc cgcgtcatcg gcgcgtttga aggcagtatc gaaaaaatcg
     8461 tcgcactcgc cgcgctgatg cccatcgtcg ccggcatcgg cggtaactcg ggcaaccaga
     8521 cgattaccat gattgtccgc gcgatggcga tggggcagct gacggatatg caggcgggaa
     8581 ggctgttgaa aaaagaagtc ggcgtcgcct tggtcaacgg cattatttgg gggacggtga
     8641 tgggagcggt ttcgtggctg ctttacggca gcctcggcat cggtttggtc atggttgccg
     8701 cgatgacgct caacctcctg ctggcggcaa cggtcggcgt attgattccc gtggtaatgg
     8761 aaaaattcgg acgcgatccc gcgctgggca gctcggtgct gattaccgcc gttaccgact
     8821 ccggcggctt cctgattttc ttgagtctcg ccacgctgtt cctgctttaa atgccgtccc
     8881 gacccgcgca aaaatgccgt ctgaagcaga agctgcttca gacggcattc gaccattcat
     8941 ccttgttgct caagattatt ggacggtatg tcggggcagc cctttggcaa cgccgaccac
     9001 atcctccccg aacagcgcgt tgacatcggt ttcatcaaac acgtatttgc tgtggcagaa
     9061 gtcgcaatcg acttcgatgc tgccttgttc cgccaccacg ccgccgactt cttccccgcc
     9121 cagcatcaac agcatatcgc tgaccttgcc ccgagaacag gtacatgagg attcaaacgt
     9181 ttccggctcg aacacgcgcg gcggcgtttc gtggaacagg cggtataaaa cgtgttgcgc
     9241 gtccaattct gccagctcct ccgccgtcag cgtgcgcgcc agcgtactga cgtgttccca
     9301 tgcctcttca tccaacacct cttcaggcag acgctgcacc agcagcccgc ccgccgcttc
     9361 atcgcttgca gacagggcga tgtgcgtatc aagctgttcg gaacgtttca tatagttcac
     9421 caacatttgc gcgatgctgc cgccttccaa aggcactacg ccctgccagg gttcgccgtc
     9481 tttgggttgc agcgtcaata cgaacacgcc gttgccgccc aaaaggtcgc cgaggctttc
     9541 gtcatcggct atttctgcgg tttcgtccca ccgcgcggtt gcacggacgg tacggtcgga
     9601 agtcgcttcc gcaaccagca ttttcagttt tccctgcccc tgaacctgca caatcagcgt
     9661 gccttcgttt ttaaggttgc ccgacagcag aacgccggca gccagtagct ctcccaaggc
     9721 gcagcggatg gcggcgggat agtttttctg ttttacaatg tgctgccaca cgttttccag
     9781 acggacgtgc agcccgcgca cgggcatatc gtcgaagata aagcgggtac gcgcatcggc
     9841 gcggttgatg gcggtttgat tcatgatttt ctctgactga ttgttcggat ggcggctata
     9901 tggttgcggt cggcgcgaaa acaaggcgga cggcggatgc gcttcccaaa ttctcaataa
     9961 attatataaa aatcaacata ttaactcaat ctaacaagcc gttttttgcc aaacagccgt
    10021 ttttttatat acaatcaaca agatattttc gactgataca gcataacatc gcacggcggc
    10081 acgatgcctc ctgcgcggaa acaccgatat ggattctttt ttcaaaccgg cagtttgggc
    10141 ggttttgtgg ctgatgtttg ccgtccgccc cgcccttgcc gacgagttga ccaacctgct
    10201 cagcagccgc gagcagattc tcagacagtt tgccgaagac gaacagcccg ttttacccgt
    10261 caaccgagcc cccgcccggc gggcgggcaa tgccgacgaa ctcatcggca gcgcgatggg
    10321 gcttttgggt attgcctacc gctacggcgg cacatcggtt tctaccggtt ttgactgcag
    10381 cggattcatg cagcacatct tcaaacgcgc catgggcatc aacctgccgc gcacgtcggc
    10441 ggaacaggcg cggatgggcg cacccgttgc ccgaagcgaa ttgcagcccg gggatatggt
    10501 gtttttccgc acgctcggcg gcagccgcat ttcccatgtc ggactttata tcggcaacaa
    10561 ccgcttcatc cacgcgccgc gcacggggaa aaatatcgaa atcaccagcc tgagccacaa
    10621 atattggagc ggcaaatatg cgttcgcccg ccggatcaag aaaaacgacc cgtcacgctt
    10681 tctgaactga tttcccaagg aatacgcaat gagtatgccc gaaatgccca aatggtacgg
    10741 cgatgacgga cagatcgtat cctgtaccga aaaggtcaaa gtgatgtccg aaaatatggc
    10801 cgagctgtat cagacggcac aagacgcgtt tgaagacgcg ctgctgatgg gttgcggcga
    10861 acggcagttg cgcgcttacc tgctcgcgct gattgaaggt ttggaaaatc cctaccgcaa
    10921 agtctgaaca cgccccggtt gctgcggcac ggtttatccg tgccgttttt gcgtttgtgc
    10981 gcggcttcgg cttttcagac ggcatatttg acgttatgat taaacagtta acaagattta
    11041 tcacaacgcc gtcaaaagac agacacacaa catgaacatc atccgcgcgc tcctcatcat
    11101 cctcggctgc ctcgccgccg gcgaaaccgc cgttttccta gcaggcatca aactgcccgg
    11161 cagcatcgtc ggcatgggcg tgctgtttgc gcttttgcag gcgggttggg tcaaaacgtc
    11221 ttggctgcaa cagcttaccg acgcgctgat ggcaaacctg acgctgttcc tcgtgccgcc
    11281 ctgcgtggcg gtcatcagct atttggattt gattgccgac gattggtttt cgatactggt
    11341 ttccgcctcc gccagcactt tgtgcgtact gctggttacg ggcaaggttc accgctggat
    11401 acggagcatt atctgatgag cgaaatcctc aggcagcccg gcatcctgct tttcctcacg
    11461 cttgccgtgt acgcgcttgc gattatcgtg cgcacgcgca cgggcaatat cttctgcaac
    11521 cccgtactcg tcagcactat cgtgctgatt gcctacctga aaatcctcgg tatcgattat
    11581 gcggtgtacc acaacgccgc gcaattcatt gattttcggc tgaaacccgc cgtcgtcgtg
    11641 cttgccgtgc cgctctacca aaaccgccgt aaaatcttca accagtggct gcccgtcatc
    11701 gtttcgcagc ttgcgggcag cgttacgggc attgttacgg ggatgtattt tgccaagtgg
    11761 ctgggcgcgg aacgcgaagt cgtcctcccg ctcgcgtcca aatctgttac caaccccatc
    11821 gccatcgaaa tcaccagctc catcggcggc attcccgcca ttaccgccgc caccgtcatc
    11881 attgccggtc tggtcggaca gattgccggt tacaaaatgt tgaagaacac ggtcgtcatg
    11941 ccctcgtccg tgggtatgtc gctcggcacg gcttcgcacg cgatgggcat tgccgcctcg
    12001 ctcgaacgca gccgccgcat ggcggcatac gcggggctgg ggctgacgtt caacggcgtg
    12061 ctgaccgcgc tgattgcgcc gctgcttatc cccgttttgg ggttctgaac ccgtttcaga
    12121 cggcatttca acccatgccg tctgaacgcc gacacactcg caaggagaac cgttatggct
    12181 gtcaacctga ccgaaaaaac cgccgaacaa ctgcccgaca tcgacggcat tgccctctac
    12241 accgcccaag caggcgtgaa gaagcccggg cataccgacc tgacactgat tgccgtagcc
    12301 gccggcagca ccgtcggtgc agtcttcacg accaaccgtt tctgtgccgc gcccgtccac
    12361 atcgccaaat cgcacctttt cgacgaagac ggcgtgcgcg ccctcgtcat caacacgggc
    12421 aacgccaacg cgggtacggg cgcacagggc agaatcgatg ctttggcagt gtgtgccgcc
    12481 gccgcccggc aaatcggctg caaaccgaac caggtgctgc ccttctccac cggcgtgatt
    12541 ctcgaaccgc tgcccgcaga caaaatcatc gccgccctgc ccaaaatgca gcctgccttc
    12601 tggaacgaag cggcacgcgc catcatgacc accgacaccg ttcccaaagc cgcctcgcgc
    12661 gaaggcaagg tcggcgacca acacaccgtc cgcgccacag gcattgccaa aggctcgggc
    12721 atgattcatc ccaatatggc gaccatgctc ggtttcatcg ccaccgatgc caaagtttcc
    12781 caacccgtcc tccaactgat gacgcaggaa atcgccgacg aaaccttcaa caccatcacc
    12841 gttgacggcg acaccagcac caacgacagc ttcgtcatca tcgccaccgg caaaaacagc
    12901 caaagcgaaa tcgacaacat cgccgacccg cgttacgccc aactcaaaga attgttgtgc
    12961 agccttgcgc tcgaactcgc ccaagccatc gtccgcgacg gcgagggcgc gaccaagttc
    13021 atcaccgtcc gcgtcgaaaa cgccaaaacc tgcgacgaag cccgccaagc cgcctacgcc
    13081 gcggcacgtt cgccactggt caaaaccgcc tttttcgcct ccgaccccaa cctcggcagg
    13141 ctgctcgccg ccatcggtta tgccgacgtt gccgacctcg ataccgacct cgtggaaatg
    13201 tatctcgacg atattttggt tgccgaacac ggcggacgcg ccgcaagcta caccgaagca
    13261 caagggcagg cggtgatgtc gaaggacgaa atcaccgtcc gcatcaagct gcatcgcgga
    13321 caagccgccg ccaccgtcta tacctgcgac ctgtcgcacg gatacgtttc catcaacgcc
    13381 gactaccgtt cctgacccga cacggcttca gacggcatac ataaaatgcc gtctgaaccg
    13441 tcggacaaca taccatgacc tccacattcc cccgccgcct cgcccgcaaa atccgccaaa
    13501 cccgccgcct gtcgcgcaaa agcatcgcct ttctgttcct tctggcaggt tcggcactcg
    13561 tcgccctgac cgcactgttt tttgcccatc ttgccgattt tgcgctggaa ctgaacgcca
    13621 aactggttca acaatacccg tggttcgcat ggattgccct gccgccggga ctgcccgtca
    13681 tcgtctggct cacacgcaaa ttcgccccct tcaccgccgg cagcggcatc cctcaagtca
    13741 tcgcctcact gtcgctgccc tacggcgcac aaaaaacgcg gctgatacgc ctcgggcaaa
    13801 cgctgctgaa gattccgctg acctttttgg gtatgctgtt cggcgcgtcc atcggacgcg
    13861 aaggcccgtc cgtgcaggtc ggcgcggcag taatgggcgc gtggggcgcg tggtgcaaaa
    13921 aacacggttt ggcgtttaag ggtatgcagg aaaacgattt gatggcggcg ggcgcggcgg
    13981 gcggtttggc agcagcgttc aacgcgccgc tggcgggcgt gattttcgcc attgaggaac
    14041 tggggcgcgg catcatgctg cgctgggagc ggcagattct gctcggcgta ctcgccgccg
    14101 gtttcataca ggtcgccatt cagggcaaca acccgtattt ttcgggtttc aacggcggcg
    14161 ttttggaaaa tattttgatg tggatcgtgg cggcggggct ggtttgcggc gcggctggcg
    14221 gcatatttgc ccgtatgctc catcgcggtg cggcggcgtt tgcaccgcgc aagatacgcg
    14281 gcttcatccg caaccgtccg ctgctgctgg cggcactgat ggggctgctg ctcgccctgc
    14341 tcggcacgtt ctaccaaggc aaaacctacg gcaccggcta ccacgaagcc gcccaagccc
    14401 tgcacggcat ctacgaagcc cccgtcggac tcgccgccgc caaatggctc gccaccgtat
    14461 tcagctattg ggcaggcatc ccgggcggca ttttcactcc ctcgctgacc ataggtgcgg
    14521 ttttgggcga gcatatcgcc gccatcgccg acatatcgca gggtgcaaac atcatcgtcc
    14581 tcatctgcat ggcggcattt ttagcgggcg cgacacaatc gccgattact tccgccgtcg
    14641 tcgtcatgga aatgaccggc gggcaaagcc tgctgttttg gatgctgatt gcctgcattt
    14701 tcgcctcgca ggtttcgccg cgtccgttct accacgcatc gggaatgcgc ttccgccggc
    14761 gcgtgcttca ggaaaccgcc gcccaaaccg gcaatgcgcc cgcaagaccg caagccgcaa
    14821 acagcaaaac gggaatgccg tctgaaaatt aaaacgcccc cgatcaaacg ccggcagccg
    14881 ccttgattca aataccgttc cgctgcgctt gaaatttcag caacaatgcc gtctgaacga
    14941 cagaatgcgg ttttcagacg gcatttcccc atcccgatat tgttcaaaca aaaccgaagc
    15001 gtttgctata attgcacttt ttaccgcaca cgcacgccca tgtttcccga tttctcccaa
    15061 accctctcca aagaccgcca cttcctgcaa tccgccttca aaaatcccaa caaatacggc
    15121 ggcttgtcca aaatcgaaga aaaataccga aaatcgcacg aaatcttctt gaaacgtttg
    15181 gccgcattgc caaaacccga attcgacaac accctgcccg ttcacgaaaa actcgaagaa
    15241 atcaaaaaaa ccgttgccga aaatcaggta acgattatct gcggcgaaac cggttcgggc
    15301 aaaaccacgc agttgcccaa gatttgtttg gaactcgggc gtggggcggc aggattgatc
    15361 gggcataccc agccgcgccg tttggccgcg cgttcggtgg cggagcggat tgccgaagag
    15421 ctgaaatccg aaatcggcag cgcggtcggc tataaagtgc gctttaccga ccacacctcg
    15481 cgcgatgcct gcgtcaagct gatgaccgac ggcatcctgc tggcggaaac ccagaccgac
    15541 cgttatctcg ccgcctacga cacgattatc attgacgaag cgcatgaacg cagcctgaac
    15601 atcgacttcc tcttgggcta cctaaaacaa ctcctgccgc gccgccccga tttgaaagtc
    15661 atcatcacat cagccaccat agacgcagaa cgcttctccc gacacttcaa cggcgcgccc
    15721 gtactggaag tgagcgggcg cacctatccc gtcgaaatcc tctaccgacc gctgaccggc
    15781 aaagacgaag acgacgcaga agtcgagctg accgacgcga ttgtcgatgc cgccgacgaa
    15841 ttggcacgct acggcgaagg cgatatttta gtattcctgc cgggcgaacg cgaaatccgc
    15901 gaagcggcag aagccctgcg caaatccaca ctgcgccgca atgacgaaat cctgcccctg
    15961 tttgcacgcc tgtcgcacgc cgaacagcac aaaatcttcc acccttcagg cgcaaaatgc
    16021 cgcatcgtgc tggcgaccaa cgtcgccgaa acctcgctca ccgtgccggg catcaaatac
    16081 gtcatcgaca ccggcctcgc gcgcgtcaaa cgctattccg cgcgggcaaa agtggaacaa
    16141 cttcacgtag aaaaaatctc ccaagccgcc gcccgccaac gctccggccg ctgcggacgc
    16201 gtctcagcag gcgtgtgtat ccgactgttt tcagaagaag attttaacag ccgtcccgaa
    16261 tttaccgatc ccgaaatcgt ccgcagcaac ctcgccgccg tcatcctgcg catggcatcg
    16321 ctgaacttgg gcgacgtagc agcattcccg tttttagaaa tgcccgactc gcggtatatc
    16381 aatgacgggt ttcaggtgtt gttggaattg ggggcagtgg aggttgtctg aaaacaggca
    16441 gatcgggcgt ttatgcccaa cgagaacctt attgtcacta ttattaggga ttaatacaat
    16501 gaatataaaa gaatttatgt ctaactatac caaccatccc gttctcttta ttggaacagg
    16561 tatgagtttg agatacttag ataattcata tacttgggat ggtttattat ctaaaattgc
    16621 aatagattta tttggagatg atagggaata tttgaacatc aaatcacggt actgtgaaga
    16681 tggtagattc caatatgaag agattgcaga ggaattacaa agtaaatttg ataaagtttt
    16741 agaaaatgac cctgatggta gatttaaaga aataaatgat aagttctttg aaaatatgag
    16801 ggcgggaaac accttgagca ggtttaagat ttatatatcc accctgctct cacaattgaa
    16861 ttataaagat aattctaata cagaattatc tgaattaaaa aaagcgagaa aaaatgtagg
    16921 gtcaatcatt acaacaaatt atgataaatt agcccaagat atttttgaat ttaacccact
    16981 aattggtaat gatattcttt taagcaaccc ttatggctca gtatacaaaa tacatggttg
    17041 tgtggacgat ccatcaaaaa ttattattac caaaaaggat tatgagaaat ttaaagaaaa
    17101 atatgaactt attagagccc aactattatc gttatttatt cacaatccaa tcatatttct
    17161 tggatataat gttggtgacg aaaatatcaa agaaatttta aaaacaatct ttacttatgt
    17221 agaaccaaac tctccttcag ctaataaaat tcgtaggaat tttttactcg tagaatatga
    17281 acctgagtct aacaatgaag atattgttga acatgatata gatataactg gattctctac
    17341 tatccgtatt aataaaatca aaacagataa cttctcacaa atttataaag ctcttgcaga
    17401 actaacacta ccaatctcag ctatggatgt acgtaaattt caatctatag caaaggagat
    17461 ttatactggc ggtaacatta aagttagctt tacggaagat atggacaatt taaataatag
    17521 cgataaagtg gttgctattg gttcaactaa aactatcagc tacaactttc aaacaacatc
    17581 agagatgatg tcaaattatt tcaaaataat tgaagaggaa aattcacaac ttctaaaatt
    17641 aatagataaa catagtatag catctacgca atattttcct atttatggat ttagtaggat
    17701 atgttctgat atacataaag aagctgtact aaaacgccaa caaaaagaaa aattagatca
    17761 ttttattgaa gaaataaata ggcgttgtaa aaataatcat tcatccatcc aatcaatttt
    17821 agatgatgaa aatatttcag acacatataa aaacgatgcg attgcttggg gaatatggaa
    17881 taaccaactt tcagaagatg aggttgaaaa ttatttaaaa aattttgtga ataaaaaaaa
    17941 tacgcactat aaaagactac tatgtatgtt cgattataaa aaatatgcgg atactgtcta
    18001 aactctaaaa aataaggtcg ggcatttatg tccgacctac aaaactatgc aaaacactaa
    18061 aaatcaagca cgcagctcag gcattcatgc ccaacacaat ccgacaaatg acaaaccact
    18121 ttcagacaac ctcgtttttt gaaaaacaat ctgtaaacag atagctgctg aagaataccg
    18181 ttgccgagcc ccaaaacccg tcctgcaact tttattgtga actgcccatt atgaaaaaat
    18241 cccttttcgt tctctttctg tattcatccc tacttaccgc cagcgaaatc gcctatcgct
    18301 ttgtattcgg aattgaaacc ctaccggctg caaaaatggc ggaaacgttt gcactgacat
    18361 ttgtgattgc tgcgctgtat ctgtttgcgc gttataaggc aacgcgtttg ttgattgcgg
    18421 tgtttttcgc gttcagcatt attgccaaca atgtgcatta cgcggtttat caaagctgga
    18481 tgacgggtat taactattgg ctgatgctga aagaggttac cgaagtcggc agcgcgggcg
    18541 cgtcgatgtt ggataagttg tggctgcctg ctttgtgggg cgtggcggaa gtcgtgctgt
    18601 tttgcagcct tgccaagttc cgccgtaaga cgcatttttt ctgccgatat cctgtttgcc
    18661 ttcctaatgc tgatgatttt cgtgcgttcg ttcgacacga aacaagagca cggtatttcg
    18721 cccaaaccga catacagccg catcaaagcc aattatttca gcttcggtta ttttgtcgga
    18781 cgcgtgttgc cgtatcagtt gtttgattta agcaagatcc ctgtgttcaa acagcctgct
    18841 ccaagcaaaa tcgggcaagg cagtattcaa aatatcgtcc tgattatggg cgaaagcgaa
    18901 agcgcggcgc atttgaaatt gtttggttac gggcgcgaaa cttcgccgtt tttaacccgg
    18961 ctgtcgcaag ccgattttaa gccgattgtg aaacaaagtt attccgcagg ctttatgacg
    19021 gcagtatccc tgcccagttt ctttaacgtc ataccgcacg ccaacggctt ggaacaaatc
    19081 agcggcggcg ataccaatat gttccgcctc gccaaagagc agggctatga aacgtatttt
    19141 tacagtgccc aggctgaaaa ccaaatggca attttgaact taatcggtaa gaaatggata
    19201 gaccatctga ttcagccgac gcaacttggc tacggcaacg gcgacaatat gcccgatgag
    19261 aagctgctgc cgttgttcga caaaatcaat ttgcagcagg gcaggcattt tatcgtgttg
    19321 caccaacgcg gttcgcacgc cccatacggc gcattgttgc agcctcaaga taaagtattc
    19381 ggcgaagccg atattgcgga taagtacgac aacaccatcc acaaaaccga ccaaatgatt
    19441 caaaccgtat tcgagcagct gcaaaagcag cctgacggca actggctgtt tgcctatacc
    19501 tccgatcatg gccagtatgt gcgccaagat atctacaatc aaggcacggt gcagcccgac
    19561 agctatattg tgcctctggt tttgtacagc ccggataagg ccgtgcaaca ggctgccaac
    19621 caggcttttg cgccttgcga gattgccttc catcagcagc tttcaacgtt cctgattcac
    19681 acgttgggct acgatatgcc ggtttcaggt tgtcgcgaag gctcggtaac aggcaacctg
    19741 attacgggcg atgcaggcag cttgaacatt cgcaacggca aggcggaata tgtttatccg
    19801 caataagcag cgtgaaaacc aataaagaca aatttagatg atgtcgggga agatgcccga
    19861 ccgacaagac tatgcaaaat atgaaaaacc aagtacgcgg atcaggcatg gatgcccgat
    19921 ccaacccggc caatgtttca gacggcctgc aaaacagttc gggccatatc ggtaccaaca
    19981 cgcgctaccg cctgaccaag ctcggcgaac aaatggcgcg cctgcccatc gatccgaaaa
    20041 ttgcgcgcat tttgctggcg gcgaagaaac acgactgcat ggcggaaata ttggtgattg
    20101 cgtctgcgct gtcgattcaa gacccgcgcg aacggccgct ggaagcgcgc gatgccgcgg
    20161 ccaaggcgca tgaacgcttt accgacaagc aatccgattt ccttacctac ctgaatattt
    20221 gggacagttt ccagcgcgag cgcgacaaag gtttgtccaa caagcagttg gtgcaatggt
    20281 gccgtcaata ttttctgtcg cacctgagga tgcgcgagtg gcgcgagctg caccaccagc
    20341 ttgcccaaac cgcgattgaa atgggcttga ccaccaaaga agctgcgttt agacgacctc
    20401 ccgaagtcaa gcaactgacg tcgtctgaga atgcgggcga ccaagaccta tctgccaaac
    20461 tcaaacaaaa acaactggat aagaaacaac accgcaccca aatccgcgct accaaagaag
    20521 cgggctacga acaaatccac cgcgccctgc tcaccggcct catcgccaac gtcggcatga
    20581 aatcgcccga cagcaacgac tacaccggcg cgcgcggcag ccgcttccac cttttccccg
    20641 cctccgccct gttcaaagcc aagcccaaat gggtgatggc ggcagaattg gttgaaacca
    20701 cgcgccttta cgcgcgcgac gtcgccgtta tccagcccga atggatcgag caggaagccc
    20761 cgcaccttgt ccgctaccat tatttcgagc cgcactggga acaaaaacgc ggcgaagtcg
    20821 tcgccagcga acgcgtaacg ctttacggcc tggccgtatt gccgcgccgc cccgtgtctt
    20881 acggtaaagt tgcgcccgaa gaagcacgtg aaatctttat ccgcagcgcg ttggtggcgc
    20941 aggaatacga tttgaaagcg gatttttttg tccacaacaa aaagctgatt aaagaaatta
    21001 ccgaactcga acacaaatcg cgcaagcaag acgtattggt cgatgacgaa gccctgtttg
    21061 cgttttatca cgaacggctg cccgattttt atatggcgga ttcggtttca gagggcctgt
    21121 gtcctgcaaa tccgcagcaa accaccccct cccccgtggg ggaaggctgg ggagagggca
    21181 aaacagttgc cgcacaaacc aacttttccg caacctcagc aagccctctc cctaaccctc
    21241 tcccacagga gagggaacag agtgcctcag tttcaacggt ttcaggcagt ctgaaaacaa
    21301 tgtcttgcga agcaaggctg aatttctgcg aagctaaaac taaaaccgaa agcagcctgc
    21361 actcccaaag gctatctgaa aactacaccc cgccattttc agacggcctg cgtcctgcaa
    21421 acccccagca aaccaccccc tcccccgtgg gggagggctg gggagagggc aaaacagttg
    21481 ccgcacaaac caacttttcc gcaaccgcag caaaccctct cccacaggag agggaacaga
    21541 gtgcctcggc tttaacagtt tcagatgacc ccaaacccaa aaagcagcct gcatctcaaa
    21601 aaggccgtct gaaacctttg ccccttgccg atatccgcac ctttgaagcc tggctcaaaa
    21661 ccgccgagcg cgacaacccg cgcctgttgt tcctcagccg cgacgacctc atgcagcacg
    21721 ccgccgcgca catcaccgaa gaacaattcc ccaaacactg gcagaccgca gacggcaaat
    21781 tcaaactcag ctaccgcttc gagccgcacc acccgctcga cggcgttacc ctcaccctgc
    21841 cgctcaccgt gctcaaccgc atcagccccg ccgccctcga atggctcgtg cccggcatga
    21901 tacgcgaaaa aatccagcta caaatcaaag cactgcccaa acaaatccgc cgcatctgcg
    21961 tacccgtacc cgaattcatc acccaatttt taagccaaaa ccccgaccgc aacgccccca
    22021 tcctgcccca gctcgcccaa gccatcgcca aaaccgcagg cgacatccgc atactcgagc
    22081 aaatcaacca agacgaatgg gccgcgttca ggctgcccga acactgctat ttcaacctcc
    22141 gcatcatcga cgacggcgga caagagttag ccatgggtcg cgatttaatc caaatccaac
    22201 aacaacttgg caaagccgcc accaccacct tccgcgacaa cacccaagaa ttcgagcgtg
    22261 acaacgttac cgcatgggac atcggcatcc tgcccgaatc catcaaattc gcccgcggca
    22321 aacaacagct caccggctac ctcggcctgc aaaaagaaaa agacggccgc atcgccctgc
    22381 gcctgttcga cacgtctgcc gccgccggac acgcccacag attaggcgtg attgaactca
    22441 tgaaactaca actaaaagaa caagttaaag atctgaacaa aggcatccaa ggcttcaccc
    22501 aagccgccat gctgctcaaa cacatcaacg ccgacaccct gcgcgacgac ctcacccaag
    22561 ccgtctgcga ccgcgccttt atcggcgaag acaagctgcc gcgcaacgaa aaagccttca
    22621 aagaacaaat caaacgcgcc cgcagccgcc tgcccgccgt caaagaagcc ctcagccgct
    22681 acctgcagga aaccgcagcc gcctacgccg aactgaacgg caaactcggc aaacacccat
    22741 tgacccacct cctaagacta cgcctgcaaa ccctgcttgc acccggcttc gccacccgca
    22801 ccccgtgggc acaatggccg cgcctcccca tctacctcaa agccatgacc ctgcgcctcg
    22861 aaaaatacag cagcaacccc gcccgcgacg cagcccgcga agccgatatc caagagctgg
    22921 aacaaatgtg gcaggaaaaa actgacggct tggcgaaaca aggacagccc gtttcagacg
    22981 gcctcgccgc gtttaaatgg atgattgaag aattgagagt gtcgctgttc gcgcaggagt
    23041 tgaagacgcc gtatccggtg tcggtaaagc ggctgttgaa gatgtg
//
LOCUS       DAWTQS010000029        22345 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.029, whole genome shotgun sequence.
ACCESSION   DAWTQS010000029 DAWTQS010000000
VERSION     DAWTQS010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22345
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.029"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            <1..282
                     /locus_tag="WNA25_001110"
     CDS             <1..282
                     /locus_tag="WNA25_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFA9823874.1"
                     /translation="FAGLSPQQKESGTSVRGKGKLTKFGNRKLRAVLFMPAMVAYRIR
                     AFPGFIKRLEEKKKPKKVIIAALMRKLAVIAYHVHKKGGDYDPSRYKSA"
     gene            complement(415..870)
                     /locus_tag="WNA25_001111"
                     /pseudo
     CDS             complement(415..870)
                     /locus_tag="WNA25_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750392.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
     gene            1122..3584
                     /gene="lon"
                     /locus_tag="WNA25_001112"
     CDS             1122..3584
                     /gene="lon"
                     /locus_tag="WNA25_001112"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFA9823875.1"
                     /translation="MTQKEKHFEEYAALATLPLRDVVVYPHMVLPLFVGRPKSIAALE
                     NAITREEPVFLLAQTDAAVEEPVAADLYQTGTVAQVLQVLKLPDGTVKVLVEGLYRGR
                     VLTIEDTGGLFVSHIEAVVEEDTGGNTDLEAVRRTLLAQFEQYAKLNKKIPAEIIGSI
                     NGIAENSRLTDTVAAHLQLKLAQRQQILEIPEIGKRMEFLLAKLESELDIMQAEKRIR
                     GRVKRQMEKSQREYYLNEQIKAIHKELGEEDENGELDALEAGIKKAGMTKEAEEKCLS
                     ELKKLKMMPPMSAESTVVRNYIDTLLGLPWKKKSRVSKDIAKAGLVLDADHYGLEKVK
                     ERILEYLAVQKRMDKLKGPILCLVGPPGVGKTSLGESIAKATGRKYVRMALGGVRDES
                     EIRGHRRTYIGSMPGKILQNMAKAGVRNPLFLLDEIDKLGSDFRGDPASALLEVLDHE
                     QNNKFADHYAEVDYDLSDVMFIATSNSLNIPTPLLDRMEIIRLSGYTEDEKINIAMQY
                     LVPKQMKRNGVKEGELVVEESAVRDIIRYYTREAGVRSLDREIAKICRKVVMQITLNE
                     DKKRLSETKKTSKAKPRAVKVNEKNLHDYLGVRRFDYGVAESENRIGQVTGLAWTEVG
                     GELLTVEAAALPGKGMIQCTGQLGDVMKESVSAAWSVVRSRAESVGLAPDFYEKKDIH
                     VHVPEGATPKDGPSAGIAMTLAMVSAFTKIPVRADVAMTGEITLRGEVLPIGGLKEKL
                     LAALRGGIKHVLIPKDNVKDLEEIPENVKTGLTIHPVKWIDEVLALGLESRPESWAEP
                     SAAEAAAESASKPKPRSRAIKH"
     gene            3766..4035
                     /locus_tag="WNA25_001113"
     CDS             3766..4035
                     /locus_tag="WNA25_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFA9823876.1"
                     /translation="MNKSELIEAIAQEADISKAAAQKALDATTNAVTNALKQGDTVTL
                     VGFGTFYVGERAERQGRNPKTGEPLTIAAAKTLKFRAGKALKDAL"
     gene            complement(4211..4645)
                     /locus_tag="WNA25_001114"
     CDS             complement(4211..4645)
                     /locus_tag="WNA25_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823877.1"
                     /translation="MTEPKHETPTEEQVAARKKAKAKIRTIRIWAWVILALLASTALL
                     SQCAMSKPQAKQKIVESCMKNIPFAEKWQNDLKARGLDADNTRLAVDYCKCMWEQPLD
                     GLSEKQISSFGKLGAQEQLDLLGGANAFETRDKQCVADLKAD"
     gene            complement(4638..5945)
                     /locus_tag="WNA25_001115"
     CDS             complement(4638..5945)
                     /locus_tag="WNA25_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HFA9823878.1"
                     /translation="MKPVNIGLLGLGTVGGGAAAVLRDNAEEISRRLGREIRISAMCD
                     LSEEKARQICPSAAFVKDPFELVARKDVDVVVELFGGTGIAKEAVLKAIENGKHIVTA
                     NKKLLAEYGNEIFPLAEKQNVIVQFEAAVAGGIPIIKALREGLAANRIKSIAGIINGT
                     SNFILSEMREKGSAFADVLKEAQALGYAEADPTFDIEGNDAGHKITIMSALAFGTPMN
                     FSACYLEGISKLDSRDIKYAEELGYRIKLLGITRKTGKGIELRVHPTLIPESRLLANV
                     DGVMNAVRVNANMVGETLYYGAGAGALPTASAVVADIIDIARLVEADTAHRVPHLAFQ
                     PAQVQAQTILPMDEITSSYYLRVQAKDEPGTLGQIAALLAQENVSIEALIQKGVIDQT
                     TAEIVILTHSTVEKHIKSAIAAIEALDCVEKPITMIRMESLHD"
     gene            6141..6512
                     /locus_tag="WNA25_001116"
     CDS             6141..6512
                     /locus_tag="WNA25_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HFA9823879.1"
                     /translation="MLFEENPIDGQFAEYECGAGGIRLAGQSFHKPVLVHKDSVCLPQ
                     CRTLSDLTPENLLSDIKPVDYPEILIIGTGAAQEFIHPKIMADFSRIGISVECMNTDS
                     AFRTLVFLHSEGRRAWAWLQP"
     gene            6589..8511
                     /locus_tag="WNA25_001117"
     CDS             6589..8511
                     /locus_tag="WNA25_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFA9823880.1"
                     /translation="MIEIKNLTLQRGLKVLLDKASAAVNPGQRVGLIGKNGTGKSSLF
                     ALIKGEITQDGGDISIPKNWRLASVSQETPDLDISALDYVLQGDAELQAFQTALRQAE
                     VQNDGMKQAEYHAKLEEIDAYTAPARAAKLLNGLGFSQEEHSRPVKSFSGGWRMRLNL
                     AQALICRADLLLLDEPTNHLDLETVLWLENHLASLPCTQIIISHDRDFLNAATTQTIE
                     LSQQKLTQYSGNYDFYQTERAQRLAQQQAAYVKQQAQIKHLQSFIDRFKAKATKAVQA
                     QSRMKALAKLERIAPAHLDSEFSFEFYNPDHLPNPLLKLEHADLGYEGKTVLHDITLS
                     LESGARYGLLGVNGSGKSTFIKALAGKIDLLSGSIVHSEKLNIGYFAQHQLDTIRADQ
                     SPVWHIQQLSPEVREQEIRNFLGGFDFVGDMALQKTEPFSGGEKARLALAMIIWQKPN
                     LLLLDEPTNHLDLDMRHALTLALQSFQGALIVVSHDRSLLEATTDSFLLIDKGRLKNF
                     DGDLNDYRQWRLAQENAATAPAASTQSQNRKDTKRIEAQIRQEKARRSKPIQQKIDKA
                     EKEMVQLSEIQTGCEAFLTQEDAYLEANKEKLQNTLSELAKVKTQLAQIEETWLACQE
                     ELERIETEIEKQFAER"
     gene            8498..8893
                     /locus_tag="WNA25_001118"
     CDS             8498..8893
                     /locus_tag="WNA25_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HFA9823881.1"
                     /translation="MPSDKEARAGCLSLPANNGIIRTLPPPFYFTGINIRLFAALGFL
                     SLSGAAAQASIYHCNSNGKSVYTSDPSGSCADADLPKISSHQGGGYRLKIKKLSQEAK
                     IHTGNKKKNKKHAGKKNRQAAKAPKENQK"
     gene            8984..9496
                     /locus_tag="WNA25_001119"
     CDS             8984..9496
                     /locus_tag="WNA25_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823882.1"
                     /translation="MKSKLPLILINLSLISSPLGANAAKIYTCTINGETVYTTKPSKS
                     CHSTDLPPIGNYSSERYIPPQTPEPAPSPSNGGQAVKYKAPVKTVSKPAKSNTPPQQA
                     PVNNSRRSILEAELSNERKALTEAQKMLSQARLAKGGNINHQKINALQSNVLDRQQNI
                     QALQRELGRM"
     gene            complement(9857..10039)
                     /locus_tag="WNA25_001120"
     CDS             complement(9857..10039)
                     /locus_tag="WNA25_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823883.1"
                     /translation="MGVCPDARCSAAGIARQIGNGGGIHGTLAFSLLFLSLGHIGMVA
                     YHHIKGESVLPLFLLI"
     gene            10039..10374
                     /locus_tag="WNA25_001121"
     CDS             10039..10374
                     /locus_tag="WNA25_001121"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823884.1"
                     /translation="MPNLVTAVLAGRFLRWRIPYPTHQTDNISSRILTARICWYESSA
                     QAEKAVAPKTVARINCLNFMMLLLLHKAFGIVKPAAYGFAAVAALQAVLQETLKHDHC
                     IKSCSNSAK"
     gene            complement(10411..11070)
                     /gene="ung"
                     /locus_tag="WNA25_001122"
     CDS             complement(10411..11070)
                     /gene="ung"
                     /locus_tag="WNA25_001122"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFA9823885.1"
                     /translation="MDTWHDALGGEKQQPYFQEILNAVRQERLSGQIIYPPEADVFNA
                     FRLTAFDRVKVVILGQDPYHGVGQAHGLAFSVRQGVRIPPSLLNIYKELETDIEGFSI
                     PAHGCLTAWAEQGILLLNTVLTVRAGQAHSHALLGWERFTDTVIRQLATHRKHLVFML
                     WGGYAQQKGRLIDSQNHLILTAPHPSPLSAYRGFFGCRHFSQANSYLSQHGIEPINWK
                     L"
     gene            11151..11558
                     /locus_tag="WNA25_001123"
     CDS             11151..11558
                     /locus_tag="WNA25_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFA9823886.1"
                     /translation="MTVWFVAAVAVLIIELLTGTVYLLVVSAALAGSGIAYGLTGSTP
                     AAVLTAALLSALGIWFVHAKTAVGKVETDSYQDLDTGKYAEILRYTGGNRYEVFYRGT
                     HWQAQNTGQEVFEPGTRALIVRKEGNLLIIANP"
     gene            11573..12520
                     /locus_tag="WNA25_001124"
     CDS             11573..12520
                     /locus_tag="WNA25_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraslipin"
                     /protein_id="HFA9823887.1"
                     /translation="MEFFIILLAAVAVFGFKSFVVIPQQEVHVVERLGRFHRALTAGL
                     NILIPFIDRVAYRHSLKEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSN
                     YIMAITQLAQTTLRSVIGRMELDKTFEERDEINSTVVSALDEAAGAWGVKVLRYEIKD
                     LVPPQEILRAMQAQITAEREKRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAV
                     NASNAEKIARINRAKGEAESLRLVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVA
                     AFNNLAKESNTLIMPANVADIGSLISAGMKIIDSSKTAK"
     gene            complement(12627..13697)
                     /locus_tag="WNA25_001125"
     CDS             complement(12627..13697)
                     /locus_tag="WNA25_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFA9823888.1"
                     /translation="MYRRKGRGIKPWMGAGAAFAALVWLVYALGDTLTPFAVAAVLAY
                     VLDPLVEWLQKKGLNRASASMSVMVFSLILLLALLLIIVPMLVGQFNNLASRLPQLIG
                     FIQNTLLPWLKNTIGGYVEIDQASIIAWFQAHTGELSNALKAWFPVLMKQGGNIVSSI
                     GNLLLPPLLLYYFLLDWQRWSCGIAKLVPRRFAGAYTRITGNLNEVLGEFLRGQLLVM
                     LIMGLVYGLGLMLVGLDSGFAIGMVAGILVFVPYLGAFTGLLLATVAALLQFGSWNGI
                     LAVWAVFAVGQFLESFFITPKIVGDRIGLSPFWVIFSLMAFGELMGFVGMLAGLPLAA
                     VTLVLLREGAQKYFAGSFYRGR"
     gene            13882..14157
                     /locus_tag="WNA25_001126"
     CDS             13882..14157
                     /locus_tag="WNA25_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HFA9823889.1"
                     /translation="MTEQKNKASLIEFPCAFPLKVMGAVHPEFEQAVLDTVRLHAPDT
                     QAHHITTRPSSKGNYTGATVQVKVENQEQLDNIYRALTSHELVKVVL"
     gene            14159..14782
                     /gene="lipB"
                     /locus_tag="WNA25_001127"
     CDS             14159..14782
                     /gene="lipB"
                     /locus_tag="WNA25_001127"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFA9823890.1"
                     /translation="MKIIHKGLVEYLPTFEAMKTFNAGRNADTEDELWVVEHPPVFTQ
                     GLAGKPEHLLIRDDIPVVQIDRGGQITYHGPGQLVVYTMIDFKRRKTSVRNIVSALEN
                     SIIATLAEYGIEAAADPKRPGIYVGERKIASLGLRIKNGSVYHGLALNVNMDLSPFTQ
                     INPCGYAGMEMTQIADFVQPCPAPDEVASKLTAHLETQLTPKADNNE"
     gene            14775..15758
                     /gene="lipA"
                     /locus_tag="WNA25_001128"
     CDS             14775..15758
                     /gene="lipA"
                     /locus_tag="WNA25_001128"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFA9823891.1"
                     /translation="MSEIKTDDPKRGIKLRGADKTARIPIKVVPLQEKLKKPEWIRAK
                     LPSRKFFEIKDILREQKMHTVCEEASCPNIGECFSKGTATFMIMGDICTRRCPFCDVG
                     HGRPNMLDPDEPKNLAESVKAMNLRYVVITSVDRDDLRDGGAQHFADCIKAIRETSPN
                     TKIEILVPDFRGRLDIALKILAETPPDVMNHNLETHPSLYRKARPGANYQHSLDLLKR
                     YKEMMPHIPTKSGIMVGLGETDEDVREIMRDMRAHNIEMITIGQYLQPSDGHLPVLRY
                     VTPEQFKIFEKEAYELGFTNAAIGAMVRSSYHADEQAAEALRESHGGCGHH"
     gene            16233..16697
                     /gene="bfr"
                     /locus_tag="WNA25_001129"
     CDS             16233..16697
                     /gene="bfr"
                     /locus_tag="WNA25_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFA9823892.1"
                     /translation="MQGNQAVVDYMNELLSGELAARDQYFIHSRLYSEWGYTKLFERL
                     NHEMEEETTHAEDFIRRILMLGGTPKMARAELNIGTDVVSCLKADLQTEYEVRDALKK
                     GIKLCEEAQDYVTRDLMVAQLKDTEEDHAHWLEQQLRLIELIGEGNYYQSQL"
     gene            16725..17198
                     /gene="bfr"
                     /locus_tag="WNA25_001130"
     CDS             16725..17198
                     /gene="bfr"
                     /locus_tag="WNA25_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFA9823893.1"
                     /translation="MKGDRLVIRELNKNLGLLLVTINQYFLHARILKNWGFEELGEHF
                     FKQSIVEMKAADDLIERILFLEGLPNLQELAKLLIGESTEEIIACDLTKEQEKHEALL
                     AAIATAEAQQDYVSRDLLEKQKDTNEEHIDWLETQQELIGKIGLPNYLQTAAQED"
     gene            17526..17846
                     /locus_tag="WNA25_001131"
     CDS             17526..17846
                     /locus_tag="WNA25_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFA9823894.1"
                     /translation="MMGNKLTLPAELPDEQDLRAVLAYNMRLFRVNKGWSQEELARQC
                     GLDRTYVSAVERKRWNIALSNIEKMAAALGVAAYQLLLPPQERLNLMTNSADTRQVPS
                     ESGI"
     gene            17895..20453
                     /gene="glnD"
                     /locus_tag="WNA25_001132"
     CDS             17895..20453
                     /gene="glnD"
                     /locus_tag="WNA25_001132"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HFA9823895.1"
                     /translation="MPENLSSALETFKQQRNAAEAHYLKANRVSVFFREYTAAVETLL
                     AALWAEHFQNSALCLMAVGGFGRGEPYPCSDVDLAVVSPAPLSDGIQEQIARFIQTLW
                     DCKLMPSVKSGSVDELCESVRDDITGDTAFLEARFLFGNRQTADELAEKMNVQRNVAA
                     FIEAKLVEMEHRHAKSQGSGAVLEPNIKSCPGGLRDIHTLLWIAKAQGLAANLPDLLK
                     QRILTRAEAGMLSHGYRRLAHIRIRLHLNAKRAEDRLLFDLQPQVAESMGYQDENRRR
                     QSEELMRVFYRAVKTVKQLGGILTPMLRSRVSSTPMRVTLRIDDDYIQVNNQIAARHT
                     DIFFRRPEHIFKIVEIMQQRNDITALEPQTLRAWWGATRKINRSFYQNSENRRRFAGF
                     FRSGNGLTQTLRFLNLYGVLGRYLPAWEKIVGLLQHDLFHIYPVDDHILAVVRNVRRL
                     ALDMHSHELPYASALMQSFEKQDILYLAAFFHDIAKGRGGDHAVQGIADARQFAADHF
                     LTEEESDLLAWLVENHLLMSAVAQKEDIQDPGVLDAFCKRVQTHERLSALYLLTISDI
                     RGTNPKLWNAWRASLLESLFHAAGRCLAGNDGNPHALFGRRRQEAADLLTRAAVPEKQ
                     QKKLWNALGSAYFARHQSREILWHAANLVHDFEPPIVRSRILPQSDSFQVMVFMPNGP
                     RLFARLCRIFSRHGFDILAARAFITEHDYILDTFIVQIPSQHAPEDYPDIQSALEAEL
                     NSFIHGHTVAETQSCNRRISRRSRYMPIAPSITITPEEDYPDRYSVEITAVNRPFLLA
                     DMAEVFFAHNVSLRYAKISTLDERVEDSFTVFSPDLKNPKIQSSLKQALLEQLA"
     gene            20600..22063
                     /gene="guaB"
                     /locus_tag="WNA25_001133"
     CDS             20600..22063
                     /gene="guaB"
                     /locus_tag="WNA25_001133"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003767939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFA9823896.1"
                     /translation="MRIVEKAYTFDDVLLVPAHSTVLPRDVKLQTKLTREITLNLPLL
                     SAAMDTVTEARLAISMAQEGGIGIIHKNMPPEMQARAVSKVKRHESGVVKDPVTVAPT
                     TLIREVLEMRAQRKRKMSGLPVVENGKVVGIVTNRDLRFENRVDLPVSAIMTPRERLV
                     TVPEGTSIDEARELMHTHKVERVLVLNEKDELKGLITVKDILKTTEFPNANKDSEGRL
                     RVGAAVGTGGDTDERVKALVEAGADVIVVDTAHGHSQGVIDRVRWVKETYPHIQVIGG
                     NIATAKAALDLVTVGADAVKVGIGPGSICTTRIVAGVGVPQLTAIHNVAEALKGTGVP
                     LIADGGIRFSGDIAKALAAGAYSVMLGGMFAGTEEAPGEIELYQGRSYKSYRGMGSLG
                     AMSQGSADRYFQDKTDSTDKYVPEGIEGRVPYKGPIVNIIHQLTGGLRSSMGYLGCAN
                     IAEMHEKAEFVEITSAGMSESHVHDVQITKEAPNYHR"
BASE COUNT         5965 a         6148 c         5445 g         4787 t
ORIGIN      
        1 ttcgcaggct taagcccgca acaaaaagaa tccgggacaa gcgtaagggg aaaaggcaaa
       61 ctgaccaagt ttggcaacag gaaattacgc gccgtcttgt ttatgccggc catggtcgca
      121 taccggataa gggcatttcc cggcttcatc aaaaggctgg aagaaaagaa gaagcctaaa
      181 aaagtcatca tcgcagcatt gatgcgtaaa ctcgccgtta ttgcgtatca cgtacataag
      241 aaaggcggag attacgatcc atcgcgttac aaatcggcgt aaatcccgaa aggaaaaaag
      301 gcatttttta aatgcctgct ttgccgcgtc tgaaatccgg tgaattttca aatattgaaa
      361 ttcaatgggt tgaaaatgaa ttgtaaagat gctgttgtca attaaagtag tatctttctt
      421 atgccactca tgcacaccca taaaccccag cagcagattg agcggcagaa aaaacggttt
      481 gccgtccgcc tctccgtaca cgtcgagcaa agcggcgcgt atatcgggcg cgcgtttgtt
      541 gtccaacacg aaaccggggc ggatttcaac ggcaagcata ttcagaatac ggctctgttg
      601 cgcctgcttc atacggtggg cgtggaaaag ggcggcgata tccgcatcgg aacggacggc
      661 ggcaggtttg cggaccttct tcttcattgc cgaaagcacc tgtttggcat tgtggaaatc
      721 gccctgccag acagttgcaa tattttgata ggtagccttc aaaatgccgt ctgcaccgct
      781 ttcgcggaca taataccaac ctttgggcgg cttttgcaga ctttcgttgc gccattcgaa
      841 cccgttatcg ggaaaaataa aagaagacat gggatacctg cgtcatgttt tgaaaatagg
      901 gcggcagaac cgcaaaccat acggatggta cagcaaggag cggcaacaca gaactgtttt
      961 tttgttcccg ccttgtcttt ccaagcccat gccgtctgaa gccggaatgt ttcagacggc
     1021 atggcatcaa actccataaa taaaccgcgt atgcttgaaa taatactttc aaccccaatt
     1081 tacgcggcaa tccgtatcct gtcagacaca agagagtatc catgacacaa aaagaaaagc
     1141 attttgagga atatgctgct ttggcgaccc ttcctttacg ggatgtcgtc gtttacccgc
     1201 atatggttct gccgctgttc gtcggcaggc cgaaatccat cgccgcactg gaaaacgcca
     1261 ttacccgcga ggaaccggtt ttcctgttgg cgcaaaccga tgcggcggta gaagaaccgg
     1321 ttgccgccga cctgtatcag accggtaccg tcgcacaagt cctgcaagtg ttgaaactgc
     1381 ccgacggcac ggtaaaagtg ctggtcgaag ggctgtatcg cggacgtgtt ttgaccatcg
     1441 aagacacggg cgggctgttc gtttctcata tagaggcggt cgtggaagaa gacacgggcg
     1501 gcaataccga cctcgaagcc gtgcgccgca ccctgttggc gcagtttgaa caatacgcca
     1561 aactcaataa aaaaatccct gccgaaatta tcggcagcat caacggcatt gccgaaaaca
     1621 gccggctaac cgatacggtc gcagcgcatt tgcagttgaa actggcgcaa cgccaacaga
     1681 ttttggagat tcccgaaatc ggcaaacgga tggaattcct gctggcaaag ctggaatccg
     1741 aactcgacat tatgcaggcc gaaaaacgca tacgcggacg cgtcaaacgc caaatggaaa
     1801 aatcccaacg cgaatattat ctgaacgaac agattaaagc gatacacaaa gaattgggcg
     1861 aagaagacga aaacggcgaa ctggatgcct tggaggcagg catcaaaaag gcgggtatga
     1921 ccaaagaagc ggaagaaaaa tgcctgtccg aactgaaaaa actcaaaatg atgccgccga
     1981 tgtctgccga atccaccgtc gtacgcaact acatcgacac cctgctcgga ctgccgtgga
     2041 agaaaaaatc ccgcgtcagc aaagacatcg ccaaagccgg actggtgctg gatgccgacc
     2101 actacggcct ggaaaaagtc aaagaacgga ttttggaata cctcgccgtc caaaaacgta
     2161 tggacaagct caaaggtccg attctgtgtt tggtcggccc tccgggcgtg ggcaaaacct
     2221 ctttgggcga atccatcgcc aaagcaacgg ggcggaaata tgtccgtatg gccttgggcg
     2281 gcgtgcgcga cgaaagcgaa atccgcgggc accgccgtac ctatatcggc tctatgcccg
     2341 gcaagatttt gcagaatatg gcaaaagcag gcgtaagaaa cccgttgttc ctgctcgacg
     2401 aaatcgacaa attgggcagc gactttcggg gcgatcccgc cagcgcgttg cttgaagtgc
     2461 tcgatcacga acaaaacaac aagttcgccg atcattatgc cgaagtggat tacgatttga
     2521 gcgacgtgat gtttattgcg acttcaaaca gcctgaatat cccgaccccg ctgcttgacc
     2581 gtatggaaat catccgtctg tccggctata ccgaagacga gaaaatcaat atcgcgatgc
     2641 agtatctcgt accgaagcaa atgaaacgca acggcgtgaa agaaggcgaa ttggtggtcg
     2701 aagaaagcgc ggtacgcgat attatccgtt attacacccg agaggcgggc gtgcgttcgc
     2761 tcgaccgcga aattgccaaa atctgccgta aggtggtgat gcagattacc ttgaacgaag
     2821 ataagaagag gttgtctgaa accaagaaaa ccagcaaagc caagcctagg gcggttaaag
     2881 tcaatgagaa aaacctacat gactacttgg gcgtgcgccg cttcgattac ggcgttgccg
     2941 aaagtgaaaa ccgcatcgga caggttaccg gtttggcttg gacggaagtc ggcggcgaat
     3001 tgctgactgt cgaagccgca gcattaccgg gtaaaggcat gatccagtgt accggccagt
     3061 tgggcgatgt gatgaaggaa tctgtatccg ctgcatggtc tgttgtccgt tcccgtgcgg
     3121 aatcagtagg tttggctcct gatttttacg agaaaaaaga cattcatgtc catgttcccg
     3181 aaggtgcaac gccgaaagac ggtccgagcg caggcattgc tatgactttg gcgatggtgt
     3241 ccgcctttac caaaattccg gtgcgtgccg atgttgccat gacgggcgaa attaccctgc
     3301 gcggcgaagt cctgccgatc ggcggtctga aggaaaaact gttggctgcg cttcgcggcg
     3361 gcatcaaaca cgtcctgatt ccgaaagaca atgtcaaaga cctggaagaa atccctgaaa
     3421 acgtgaaaac cggattaacc atccatccgg tcaaatggat agacgaggta ttggctttgg
     3481 gtttggaaag ccggcctgag tcttgggcag aaccttctgc cgcggaagcg gcggcggaat
     3541 ccgcttcaaa accgaaaccc cgcagcaggg caatcaaaca ttgaaacgca ggaaatgtgt
     3601 tgtaaaaatg cggtttcgtc ctgaaagcct gtaaaatagg gcgattccgt attttttgct
     3661 tgacacggca atttcagaat tgctataaag cgaaagttgc tcaagcagta caaaccccga
     3721 gtattcgggc atttcatcat tttttaagac aggaagggac tgattgtgaa caagtctgaa
     3781 ttgatcgaag cgattgctca agaagccgat atttccaaag ccgccgcaca aaaagctttg
     3841 gatgccacta ccaacgcagt aaccaacgcc ctgaaacaag gcgacaccgt tactctggtc
     3901 ggtttcggta ctttctacgt cggcgaacgt gcggaacgcc aaggccgcaa ccccaaaacc
     3961 ggcgagcctc tgaccattgc cgccgccaaa acgcttaaat tccgtgcagg taaagcattg
     4021 aaagacgcac tgtaaactgt ttttcagcaa aagccgattc cttgaagaat cggctttttg
     4081 ttttccccgc cgaatcagga taggggtttg cccgtcggat tcggatgaag ggctgcccgt
     4141 caaacccgga tggaaaaatg ccatctgaaa cgcttttccc cgcgttcaga cggcattcca
     4201 gacctgaggt tcaatcggct ttcaaatccg cgacacattg tttgtctcga gtttcaaacg
     4261 cgtttgcgcc gccgagcagg tcaagctgtt cttgtgcacc gagtttgccg aaggagctga
     4321 tctgtttctc gctcaatccg tccaaaggct gctcccacat acatttgcag tagtcgacgg
     4381 cgagacgggt attgtccgca tccaagccgc gcgctttcaa atcgttctgc catttttcag
     4441 caaacggaat atttttcatg caagactcga caattttctg ttttgcctgc ggtttggaca
     4501 tcgcgcattg ggagagcagg gcggttgaag cgagcaacgc caaaatgacc cacgcccaaa
     4561 tgcggatggt gcggattttg gcttttgctt ttttgcgcgc ggcaacctgc tcttccgtcg
     4621 gcgtttcgtg tttcggctca gtcatgcagg ctttccatgc ggatcatggt aatcggtttt
     4681 tccacacaat ccagtgcttc gatggctgcg attgccgact tgatgtgttt ttcgaccgtg
     4741 ctgtgggtca gaatcacgat ttcggcagtg gtctgatcga taacgccttt ttggattaag
     4801 gcttcgatgg acacgttttc ttgtgccaac agcgcggcga tttgccccag cgtgcccggt
     4861 tcgtctttgg cttggacgcg caggtagtag ctgctggtaa tttcgtccat aggcaggatg
     4921 gtttgcgctt ggacttgggc gggttggaac gccagatgcg gtacgcggtg ggcggtgtcg
     4981 gcttcaacca ggcgggcgat gtcgatgata tcggcaacca cggcggaagc ggtcggcaat
     5041 gcgcccgcgc ccgcgccgta atataaggtt tcgccgacca tattggcgtt gacgcgcacg
     5101 gcgttcatca cgccgtcgac gtttgccaag aggcggcttt cgggaatcag ggtcggatgg
     5161 acgcgcaatt cgatgccttt gccggttttg cgggtgatgc ccaacagttt gatgcggtag
     5221 ccgagttctt cggcgtattt gatgtcgcgg ctgtcgagtt tgctgatgcc ttcgagatag
     5281 caggcggaga agttcatcgg cgtaccgaat gccagcgcgc tcatgatggt gattttatgg
     5341 cctgcgtcgt tgccttcgat gtcgaaggtc gggtcggctt cggcataacc caatgcctgc
     5401 gcttctttca atacgtcggc aaacgcgctg cctttttcgc gcatttcgga gaggatgaag
     5461 ttgctggtgc cgttaataat gccggcgatg gatttaatcc tgtttgccgc caaaccttcg
     5521 cgcagggctt tgatgattgg gataccgccc gctactgccg cttcaaattg gacgatgacg
     5581 ttttgttttt ccgccagcgg gaagatttcg ttgccgtatt cggcgagcag ttttttgttg
     5641 gcggtaacga tgtgtttgcc gttttcaatg gctttcaaca ccgcttcttt ggcaatgccc
     5701 gtgccgccga acaattcgac gacgacatcg acgtctttac gcgcgaccag ttcgaacgga
     5761 tctttgacaa aggcggcgga cgggcagatt tggcgggctt tttcttcgct taagtcgcac
     5821 atggcagaaa tacggatttc gcgccccaag cggcgggaaa tttcctctgc gttgtcccgc
     5881 aacacggcag ccgcaccgcc gccgaccgta cctaagccta aaagaccgat gtttactggc
     5941 ttcattgtgt ctccttgtaa gccgactgaa atgtaaatat tgaaagacga aaatatccgc
     6001 tgccggtata attgtgccgc actttgaatc aaatgccgtc tgaaatcggc aggcgcgtca
     6061 gatgaaatct gccaatccta cattaattcg cctgattttg cacccctttc ggtgcagatg
     6121 atgcagcaac ggggtaaaaa atgttgtttg aagaaaatcc gatagacgga cagtttgcag
     6181 aatatgaatg cggcgcgggc ggaatccggc tggcggggca aagtttccat aaacccgtgc
     6241 ttgtacataa ggattcggtc tgcctgccgc aatgccgaac cttgtccgac ctgactccgg
     6301 aaaacctgtt gtccgacatc aaacctgttg actatccgga aatattgatt atcgggacgg
     6361 gcgcggctca ggagtttatc catcccaaaa tcatggcgga tttttcccga atcggaatca
     6421 gcgtggaatg catgaatacc gattcggcat tcaggacatt ggttttcctg cactcggaag
     6481 ggcgcagggc ttgggcttgg cttcaaccgt aaatttcccg tttcagacgg catcggcact
     6541 gactttcagg taaaatacgg gcttttcccg cccgacgatg tttccgttat gattgaaatc
     6601 aaaaacctca ccctgcaacg cggtttgaaa gtcctgctcg acaaggcttc cgctgccgtc
     6661 aatcccggtc agcgcgtcgg tttgatcggt aagaacggga cgggcaaatc cagcctgttt
     6721 gccttaatca agggcgaaat cactcaggac ggcggcgata tctcgattcc gaaaaactgg
     6781 cggctcgctt ccgtttccca agaaacgccc gatttggata tttccgcttt ggattacgtt
     6841 ttgcagggcg atgccgagtt gcaggctttt cagacggcat tgaggcaggc ggaagtgcaa
     6901 aatgacggca tgaagcaggc ggaatatcat gccaaattgg aagaaatcga cgcctatacc
     6961 gcgccggccc gcgcggcaaa attgttgaac gggctgggtt tttcgcaaga agaacacagc
     7021 cgtcccgtca aatccttttc cggcggctgg cgtatgcgcc tgaatcttgc gcaagccctg
     7081 atttgccgcg ctgatttgct cttgcttgac gaaccgacca accatttgga tttggaaact
     7141 gtcttgtggt tggaaaacca ccttgcctct ttaccctgca cgcaaatcat catttcccac
     7201 gaccgtgatt tcctcaatgc ggccaccacc caaaccattg aattgtcgca gcaaaagctc
     7261 acgcaataca gcggcaatta cgatttttac caaaccgaac gcgcgcagcg tctggcgcaa
     7321 caacaagccg catatgtcaa acagcaggcg caaatcaaac atctgcaatc ctttatcgac
     7381 cgcttcaaag ccaaagccac taaagccgtt caagctcaaa gccgcatgaa ggcgttggcg
     7441 aagctcgaac gcatcgctcc cgcacatctg gacagcgagt tttcctttga gttttacaat
     7501 cccgaccatc tgcccaatcc tttgctgaag ctggagcacg ctgatttggg ttacgaaggt
     7561 aaaaccgtcc tgcatgacat taccctgtcg ctggaaagcg gcgcacgcta cgggctattg
     7621 ggtgtcaacg gcagcggtaa atctacgttt atcaaagctt tggcaggaaa aatcgattta
     7681 ctctccggca gcatcgtcca ttccgaaaaa cttaatatcg gctatttcgc ccaacaccag
     7741 cttgacacca tccgcgccga ccaaagccct gtttggcata tacagcagct ttctcccgaa
     7801 gtacgcgaac aagaaatccg aaatttcctc ggcggcttcg attttgtcgg cgatatggcg
     7861 ttacagaaaa ccgaaccttt ttccggcgga gaaaaagccc gccttgccct tgccatgatt
     7921 atctggcaaa agccgaacct gctgctgctt gacgaaccga ccaaccatct ggatttggat
     7981 atgcgtcatg ccttaacgct cgcgttgcaa agtttccaag gtgcattaat cgtcgtatcg
     8041 cacgaccgca gcctgcttga agccacgacc gacagtttcc tcttgataga caaaggtcgt
     8101 ctgaaaaact ttgacggcga tttgaacgat taccgccaat ggcgcttggc acaggaaaac
     8161 gccgcaaccg cgccagccgc ttccactcaa agccaaaacc gcaaggatac caagcgtatc
     8221 gaagcgcaaa tccgtcaaga aaaagcccga cgcagtaagc cgatacaaca gaaaatcgac
     8281 aaagccgaaa aagaaatggt gcagctttct gaaattcaga cgggatgcga agcattttta
     8341 acacaagaag acgcttacct tgaagcaaac aaagaaaaat tgcaaaatac cttatccgag
     8401 ctggcaaaag tcaaaacaca gcttgcccaa atcgaagaaa cttggctggc ttgtcaagaa
     8461 gaattggaac ggattgaaac tgaaatcgag aaacagtttg ccgagcgata aagaagcgcg
     8521 ggcagggtgc ttatctttgc ctgccaataa cggtataatt cggacgttac caccgccttt
     8581 ttactttacc ggtataaaca tcagactttt tgccgccttg ggtttcctgt ccctttccgg
     8641 cgcggcggcg caagcctcca tataccattg caactcaaat ggcaaaagcg tatatacatc
     8701 agacccgtca ggatcgtgtg ccgatgctga tttacctaaa atcagcagcc atcagggagg
     8761 cggataccgc ctgaaaatta aaaaacttag tcaagaagcc aaaatacaca caggaaacaa
     8821 aaagaaaaac aaaaaacatg ccgggaaaaa gaacagacag gctgccaaag ccccgaagga
     8881 aaatcaaaaa taaacaaccg aaaagaaaag cccataaaac gccaagaaaa ccttacaaaa
     8941 aatcctcaaa aaatcaaatt attatccgaa tatcaaacac attatgaaat caaaactccc
     9001 cttaatccta atcaaccttt ccctgatttc aagcccattg ggtgcgaatg cggccaaaat
     9061 ctatacctgc acaatcaacg gagaaaccgt ttacaccacc aagccgtcta aaagctgcca
     9121 ctcaaccgat ttgcccccaa tcggcaacta cagcagcgaa cgctatatcc cgccccaaac
     9181 tcccgaaccg gcaccatcac cgtcaaacgg cggacaggct gtcaaatata aagccccggt
     9241 caaaacagta tccaagccgg caaaatccaa tacgccgcct caacaagcac ctgtaaataa
     9301 cagcagacgc tccattctcg aagcagaatt aagcaatgaa cgcaaagccc tgactgaagc
     9361 ccaaaaaatg ttatcacaag cacgtctggc aaaaggcggc aacatcaacc atcaaaaaat
     9421 caacgcattg caaagcaatg ttttggacag acagcaaaat atccaagcac tgcaaagaga
     9481 attgggacgt atgtaaggcc gtgttttcaa atcgaccgtt ccaaggattt gacagaagaa
     9541 atgatgaaaa agcaggagaa tttttgggat aagttgggcg atttactgtt tgcgcccgtt
     9601 gatataatgt tttggattaa aaaagtatgg gcggcatatc ctgtgtgcct gctgcctgta
     9661 tatcgtattg aaggtcaacg caacacatac agctgtttcc agtagttcgc ataatgtata
     9721 ttatgttaaa tttataatgg attgaataga tatttttaca atctcattca aaactcagac
     9781 acttaaaaaa tttaatcaga acaaataaaa aagcaaacaa aaatatccat ctttcaaact
     9841 catattttgc aataacctag ataagcaaga acagaggcag aacggattcg cctttgatgt
     9901 ggtggtaggc aaccattcca atatgtccca gcgacaaaaa cagcaatgaa aatgccagcg
     9961 tgccgtgaat tcctccgcca ttgccgattt gacgggcaat gcctgcggcg gaacagcgag
    10021 catcagggca tacgcccaat gcccaatttg gtgacggcgg tgttggcggg gcggttttta
    10081 cgttggcgga ttccctaccc tacgcaccaa acagacaata tcagcagcag gattctgact
    10141 gcacggatat gttggtatga atcgtccgcc caagcggaaa aggctgtcgc gcccaaaaca
    10201 gtggcaagaa taaattgctt gaatttcatg atgttgcttc ttttgcacaa agcgttcgga
    10261 atagtgaagc ccgcagccta tggctttgcg gcagtcgcag ctttacaagc ggtattgcag
    10321 gaaaccttaa aacacgatca ttgcatcaag agctgttcga attccgccaa atgaacctga
    10381 cacgcaaact ttgacgcttg aatacggcat tcatagcttc cagtttatcg gttcgatacc
    10441 gtgttgactc aaatagctgt ttgcctgtga aaaatggcgg cagccaaaaa aaccgcgata
    10501 tgccgacaga ggagacggat gaggcgcggt cagtatcaaa tgattttgac tgtctatcag
    10561 cctccctttt tgttgtgcat acccacccca caacatgaag acaaggtgct tgcggtgtgt
    10621 cgcaagctgc ctgatgacgg tatcggtaaa gcgttcccag cctaaaaggg cgtgcgaatg
    10681 cgcctgtcct gcacgcaccg tcaaaaccgt gttcagaagc aatatgccct gctccgccca
    10741 cgctgtcagg cagccgtgcg cgggaatgga aaagccttcg atgtcggttt ccaattcttt
    10801 gtaaatattg agtaaagacg gcggtatgcg gacaccctgc cggacggaaa atgccaaacc
    10861 gtgcgcctgc cctaccccgt gatacggatc ttgtccgaga atgacgactt tgacccggtc
    10921 gaacgctgtc agccggaatg cgttgaacac atcggcctcc ggcggataga taatttgccc
    10981 cgacaaacgt tcctgcctga ccgcatttaa aatttcctga aaatacggct gctgtttttc
    11041 gccgccgagt gcatcgtgcc aagtgtccat atctgcccta tcctttcaaa ttcggctata
    11101 ctgctgatat tgcgttattt tgtcaaacga cgcgctttgt ggggaaatgt atgactgtat
    11161 ggtttgttgc cgctgttgcc gtcttaatca tcgaattatt gacgggaacg gtttatcttt
    11221 tggttgtcag cgcggctttg gcgggttcgg gcattgccta cgggctgact ggcagcacgc
    11281 ctgccgccgt cttgaccgcc gcactgcttt ccgcgctggg catttggttc gtacatgcca
    11341 aaaccgccgt gggaaaagtt gaaacggatt catatcagga tttggatacc ggaaaatatg
    11401 ccgaaatcct ccgatacaca ggcggcaacc gttacgaagt tttttatcgc ggtacgcact
    11461 ggcaggcgca aaatacgggg caggaagtgt ttgaaccggg aacgcgcgcc ctcatcgtcc
    11521 gcaaagaagg taaccttctt atcatcgcaa acccttaaca ctcggaggaa ttatggaatt
    11581 tttcattatc ttgttggcag ccgtcgccgt tttcggcttc aaatcctttg tcgtcatccc
    11641 ccagcaggaa gtccacgttg tcgaaaggct cgggcgtttc catcgcgccc tgacggccgg
    11701 tttgaatatt ttgattccct ttatcgaccg cgtcgcctac cgccattcgc tgaaagaaat
    11761 ccctttagac gtacccagcc aggtctgcat cacgcgcgat aatacgcaat tgactgttga
    11821 cggcatcatc tatttccaag taaccgatcc caaactcgcc tcatacggtt cgagcaacta
    11881 cattatggca attacccagc ttgcccaaac gacgctgcgt tccgttatcg ggcgtatgga
    11941 gttggacaaa acgtttgaag aacgcgacga aatcaacagt accgtcgtct ccgccctcga
    12001 tgaagccgcc ggggcttggg gtgtgaaagt cctccgttac gaaatcaagg atttggttcc
    12061 gccgcaagaa atccttcgcg caatgcaggc acaaattacc gccgaacgcg aaaaacgcgc
    12121 ccgtattgcc gaatccgaag gccgtaaaat cgaacaaatc aaccttgcca gtggtcagcg
    12181 tgaagccgaa atccaacaat ccgaaggcga ggctcaggct gcggtcaatg cgtccaatgc
    12241 cgagaaaatc gcccgcatca accgcgccaa aggcgaagcg gaatccctgc gccttgttgc
    12301 cgaagccaat gccgaagcca tccgtcaaat tgccgccgcc cttcaaaccc aaggcggggc
    12361 ggatgcggtc aatctgaaga ttgcggaaca atacgtagcc gcgttcaaca atcttgccaa
    12421 agaaagcaat acgctgatta tgcccgccaa tgttgccgac atcggcagcc tgatttctgc
    12481 cggcatgaaa attatcgaca gcagcaaaac cgccaaataa gccgtgatga aaataccgtc
    12541 tgaagctctg ttttcgggtt tcagacggta tttttattta aacccgaaca gggttgtatt
    12601 ccgcttcaaa tatgtttcgg aaccgtctac ctgccccggt aaaaactgcc ggcaaaatat
    12661 ttctgcgcgc cctcgcgaag caagaccaag gttacggcgg ccaaaggcaa tccggccaac
    12721 attccgacaa agcccatcag ctctccgaac gccatcagcg aaaagataac ccaaaacggc
    12781 gacaggccga tacggtctcc tacaattttc ggcgtaatga aaaaactttc gagaaactga
    12841 ccgacggcaa aaaccgccca aacagccaag attccgttcc acgaaccgaa ctggagcaag
    12901 gctgcaacag tggcaagcag caatcccgta aacgcaccca aataggggac aaacaccaaa
    12961 ataccggcaa ccataccgat ggcaaatccc gaatccagtc cgactagcat caatcccaaa
    13021 ccgtaaacca agcccataat cagcatcacc agaagctgac cgcgcaaaaa ttcgcccaat
    13081 acctcgttca aattacccgt aatgcgcgta taagcaccgg caaaacgcct cggaaccagt
    13141 ttggcgatgc cgcacgacca ccgctgccaa tccagcagga aatagtaaag cagcaagggc
    13201 ggcagcagca ggttgccgat actgctgaca atattgccgc cctgtttcat caaaacggga
    13261 aaccacgcct taagcgcgtt gctcaactcg cccgtatgcg cctgaaacca cgcaataata
    13321 gatgcctgat cgatttccac atatccgccg attgtatttt tcaaccacgg cagcagcgtg
    13381 ttctgtataa aaccgattaa ttggggcagg cgagatgcca aattattgaa ctgcccgacc
    13441 agcataggga caataatcaa caataatgcc aacaacaaaa tcaaggaaaa caccatcaca
    13501 gacatcgaag cggatgcacg gttcaaaccc tttttctgca accattcgac caaagggtcc
    13561 aacacatacg ccagcaccgc cgcaaccgca aacggagtca aagtatcgcc gagcgcgtaa
    13621 accagccaga ccaaggcggc aaacgccgcg ccggcaccca tccacggctt gatgccccgt
    13681 ccttttctcc gatacataaa atccaccctt ccgaaaataa tattgcgcgc cagtatagca
    13741 gaaccgccgc gccgtcaaaa aagcctccgc cttcccgata gcctgttatc gtggtttagg
    13801 ctaaaatact gcacatccga tgccctctga aaggcaaacc cgccttcaga cggcataccg
    13861 tacacgataa ggcagcacat catgaccgaa caaaaaaaca aagcatccct catcgaattt
    13921 ccctgcgcct tcccattgaa agtgatgggc gcggtgcatc ccgagttcga gcaggcggta
    13981 ttggacaccg tccgccttca cgcccccgac acgcaggcgc accacatcac cacgcgtccg
    14041 agcagcaaag gcaactatac cggtgccacc gttcaggtaa aggttgaaaa ccaagaacaa
    14101 ttggacaaca tctaccgcgc gctgacttcg cacgaactgg tcaaagtggt actttgagat
    14161 gaaaatcata cacaaaggtt tggtcgaata tctgccgact tttgaagcga tgaaaacctt
    14221 taatgccgga cggaatgcag acaccgaaga cgaattgtgg gtcgtcgaac acccgcccgt
    14281 ctttacgcaa ggactggcgg gaaaacccga gcacctgctg attagggacg acatccccgt
    14341 cgtccaaatc gaccggggcg ggcagattac ctatcacggg cccgggcaat tggtcgttta
    14401 tacgatgatt gatttcaaac ggcgcaaaac cagcgtccgc aacatcgttt ccgcgcttga
    14461 aaacagcatc atcgccacat tggcggaata cggcatcgaa gcggcggcag atcccaaacg
    14521 ccccggcatt tacgtcggcg aacgcaaaat cgcctcactg gggctgcgta tcaaaaacgg
    14581 ttccgtctat cacggactcg cactcaacgt caatatggat ttaagcccgt ttacccaaat
    14641 caacccctgc ggctacgccg gtatggaaat gacgcaaatc gcggattttg tccaaccctg
    14701 ccccgcgccg gacgaagtcg cctccaaact caccgcacac cttgaaacac aactcacacc
    14761 gaaagcagac aacaatgagt gaaatcaaaa ccgacgaccc caaacgcggc atcaaactca
    14821 gaggcgcgga caaaaccgcc cgcatcccca tcaaagtcgt cccccttcag gaaaaactga
    14881 aaaagcccga atggatacgc gccaaactcc catcgcgcaa attctttgaa atcaaagaca
    14941 ttttgcgcga acaaaagatg cacaccgttt gcgaggaagc ctcctgcccc aacatcggcg
    15001 aatgcttcag caaaggcacg gcgaccttca tgattatggg cgacatctgc acccgccgct
    15061 gcccgttctg cgacgtggga cacggccgtc cgaatatgct cgaccccgac gagccgaaaa
    15121 acctcgcaga atccgtcaaa gccatgaacc tgcgttacgt cgtcatcacc tcggttgacc
    15181 gcgacgacct gcgcgacggc ggcgcccaac acttcgccga ctgcatcaaa gccatccgcg
    15241 aaaccagccc gaacaccaaa atcgaaatcc tcgtccccga cttccgaggc cgcttggaca
    15301 tcgcactgaa aatccttgcc gaaaccccgc ccgacgtgat gaaccacaac ttggaaaccc
    15361 atccgagcct gtacagaaaa gcccgtcccg gtgccaatta tcaacattct ttagacttat
    15421 taaaacgtta taaagaaatg atgccgcata tcccgaccaa atccggcatt atggtaggct
    15481 tgggcgaaac agacgaagac gtgcgtgaaa ttatgcgcga tatgcgggcg cacaatatcg
    15541 agatgattac catcggacag tacctccagc cttcagacgg acacttgcct gtcttgcgct
    15601 acgttacacc cgaacaattc aaaatatttg aaaaagaagc atacgaactg ggctttacca
    15661 atgccgccat cggcgcgatg gtacgttcaa gttaccatgc cgacgaacag gcggcggaag
    15721 ccttacgcga aagccacggc ggttgcgggc atcattaaac tcggaacacc gttccgaagt
    15781 aaaaaataat ttcctacgct gctgtctgcc gccttgtttg atgtaaacaa catcaatttg
    15841 aattgtccat gccgtctgaa agtccttcag acggcataat ttattcccaa aatccgataa
    15901 tctcaaaaga atctgtcggt taatgagaaa gattcattat aaattttgat aaaaaaccgt
    15961 gctctatttg ttttttgcta tggttaacaa gtgtaaatgt tctttattga tgagatggat
    16021 tcttagtttt agcagcaaaa aaaaccgata agaaacagca tactgatttg gaaggcatcg
    16081 gggaatacgc cctatttatt ttgataattg tttgtatttt aaatagaaaa tttgtcatgc
    16141 cgacagcttt tgcatacaat gcacgcatcc cgaacagcgt tacagaacgc cgtttaaccg
    16201 cacgaaaccc aaattaagaa aagagagata ttatgcaagg caatcaagct gttgttgatt
    16261 acatgaacga attgctgtcc ggcgagctgg cggcacgcga ccaatacttc atccactccc
    16321 gcctctactc cgaatggggc tacaccaaac tttttgaacg tctcaatcac gagatggaag
    16381 aagaaaccac acacgccgaa gacttcatcc gacgcatcct gatgctgggc ggtacgccga
    16441 aaatggcacg cgccgaactg aatatcggca cggacgtggt ttcctgcctc aaagccgacc
    16501 tgcaaaccga atacgaagtg cgcgacgctt tgaaaaaagg catcaaactg tgcgaagagg
    16561 ctcaagacta tgttacgcgc gacctgatgg ttgcccaact gaaagacacc gaagaagacc
    16621 acgcacactg gttggaacag cagctgcgcc tgatcgagct gattggcgaa ggcaactact
    16681 accaaagcca actgtaatcc gtattaggaa taaaaggagt ccgtatgaaa ggcgaccgtt
    16741 tggttatccg cgagctgaac aaaaacttag gcttgctgct ggtaaccatt aaccaatatt
    16801 tccttcacgc ccgtattttg aaaaactggg gctttgaaga actgggcgaa catttcttca
    16861 aacaatcaat cgtagaaatg aaagctgccg acgatttgat tgaacgcatc ctcttccttg
    16921 agggattgcc caatttgcaa gagctggcca agctgttgat tggtgaatct accgaagaaa
    16981 tcatcgcctg cgatttgacc aaagaacagg aaaaacacga agccctgctt gccgccatcg
    17041 ccacagcaga agcgcaacag gattatgtca gccgtgattt gttggaaaaa caaaaagata
    17101 ctaacgaaga acacatcgac tggctcgaaa cccagcagga actgatcggc aaaatcggtt
    17161 tgccgaacta cctgcaaaca gcggcgcaag aggactaaaa cacaaaccac tgccaaatac
    17221 agcagtcccc tgcatcatca gacggcatat ccgtataaac gggtatgccg tttttctttg
    17281 ccggtttacg aataaaatct aaattgattt gaatcaatac tcgataacgg tttttgtttg
    17341 ataataccga tatgaaatct agattgtttg tttgctgttt cccaatccgt cttgatttta
    17401 cctctttcct cttgatgtgt gtgtgtgttt gggtgtggct gccgccaccc cttttttttg
    17461 gcttttatgt gaagtaaaat ccgtaacagc aaatgcttaa cttttaacca tttatccgga
    17521 aacaaatgat gggcaacaaa ttgactctgc ctgccgaatt gcctgacgaa caggatttgc
    17581 gggcggtatt ggcatacaat atgcggcttt tccgcgtgaa caagggttgg tcgcaagagg
    17641 aattggcgcg gcaatgcggt ttggacagga cttatgtgtc ggcagtcgag cgcaaacgct
    17701 ggaacattgc cctgtcgaat atcgaaaaaa tggcggcggc tttgggcgtg gcggcgtatc
    17761 agttgctgct gccgccgcag gaacggttga acctgatgac caattccgcc gatacccgac
    17821 aagtgccgtc tgaaagcggt atttgaccgc cgaaccatcc gcctcccttt ttcccgccaa
    17881 tcgaaagaat aaaaatgcct gaaaacctgt cttcagcact ggaaaccttc aaacagcagc
    17941 gcaatgccgc cgaagcgcat tatttgaaag ccaaccgcgt gtcggtattt ttcagagaat
    18001 acacggcggc agtcgaaacc ttgctggcgg cattgtgggc agaacatttt caaaacagcg
    18061 cgttatgcct gatggcggta ggcggcttcg ggcgcggcga accgtatccc tgttcggatg
    18121 tggatttggc ggttgtctcc cctgccccgc tttcagacgg cattcaggaa cagattgccc
    18181 gatttatcca aactctgtgg gattgcaaac taatgccgtc tgtaaaaagc ggcagcgttg
    18241 acgaactgtg cgaaagcgtg cgcgacgata ttacggggga cacagcgttt ttagaggcta
    18301 ggtttttgtt tggcaaccgc caaacagcag acgaattggc ggaaaaaatg aacgtgcaac
    18361 gcaatgtagc ggcatttatc gaagcaaaat tggtggagat ggaacaccgc cacgccaaat
    18421 cgcaaggttc gggggcggtg ttggagccga atatcaaaag ctgtccgggc ggtctgcgcg
    18481 acatccacac cctgctttgg atagcgaagg cgcaaggctt ggcggccaac ctgcccgacc
    18541 tgctcaaaca gcggattttg acacgtgccg aagccggtat gctttcgcac ggctaccgcc
    18601 gcctcgccca catccgcatc cggctgcatt taaacgccaa gcgcgccgaa gaccgcctgc
    18661 ttttcgattt gcagccgcaa gttgccgaaa gtatgggtta tcaagatgaa aaccgccgcc
    18721 gtcaaagcga agaactgatg cgcgtgtttt accgtgcggt caaaaccgtc aaacaactgg
    18781 gcggcatcct cacgcccatg ctgcgaagcc gcgtttcctc cacgccgatg cgcgttaccc
    18841 tgcggattga cgacgactac atccaagtca acaaccaaat cgccgcgcgg cacaccgata
    18901 tttttttcag acggcccgaa cacattttca aaatcgtcga aatcatgcag cagcgcaacg
    18961 acattactgc gctcgaaccg caaaccctgc gcgcatggtg gggggcgacg cgcaaaatca
    19021 accgcagctt ctaccaaaat tctgaaaacc gtcgccgctt cgccggtttt ttccgcagcg
    19081 gcaacggact gacccaaacc ctgcgctttc tcaacctcta cggcgtgttg ggccgctatc
    19141 tgcccgcgtg ggaaaaaatc gtcggcctgc ttcaacacga cttgttccac atctatcccg
    19201 tggacgacca catccttgcc gtcgtccgca acgtccgccg ccttgccctg gatatgcaca
    19261 gccatgagct gccctacgcc tccgcgctga tgcagtcctt tgaaaaacaa gacatcctct
    19321 accttgccgc cttcttccac gacatcgcca aaggacgcgg cggcgaccat gccgtacaag
    19381 gcatcgcaga cgcgcgccaa tttgccgccg accacttcct gaccgaagaa gaaagcgacc
    19441 tgctcgcctg gctggttgaa aaccacctcc ttatgtctgc cgtcgcccaa aaagaagaca
    19501 tccaagaccc cggcgtactc gatgccttct gcaaacgcgt ccaaacccac gaacgcctca
    19561 gcgcgctcta cctcctgacc atttccgaca tacgcggcac caatcccaag ctgtggaacg
    19621 cctggcgcgc cagcctgctg gaaagcctgt tccacgccgc cggacgctgc ctcgcaggca
    19681 acgacggcaa cccgcacgcc ctcttcggcc gccgccggca ggaagccgcc gacttactta
    19741 cccgcgccgc cgtccccgaa aaacagcaga aaaaactatg gaacgcgctc ggttccgcct
    19801 atttcgcccg ccatcagtcc cgcgaaatcc tgtggcacgc cgccaacctc gtacacgact
    19861 ttgaaccccc catcgtccgc agccgcatcc taccccaaag cgacagcttt caagtgatgg
    19921 tctttatgcc caacggcccg cgcctgttcg cccgcctctg ccgcatcttc agccgccacg
    19981 gcttcgatat cctcgccgcc cgcgccttta tcaccgaaca cgactacatc ctcgacacct
    20041 tcatcgtgca aatcccctcg cagcacgccc ccgaagacta ccccgacatc caaagcgcgc
    20101 tcgaagccga actcaacagc tttatccacg gacacaccgt tgccgaaacc caaagctgca
    20161 accgccgcat cagccgccgc agccgctata tgcctattgc gccaagcatc accatcaccc
    20221 ccgaagaaga ctatccggac cggtattccg tcgaaatcac cgccgtcaac cgccccttcc
    20281 tgctcgccga catggcggaa gtcttcttcg cccacaacgt cagcctgcgc tacgccaaaa
    20341 tctccacgtt ggacgaacgc gtcgaagaca gctttaccgt cttcagcccc gacttgaaaa
    20401 acccaaaaat ccagtcctca ttgaaacagg ctttgctgga acagttggca taagcaccct
    20461 gccggttttc aaacggcatc ccgccgccac gccgtatgcc gtctgaaggc gggggatatt
    20521 gcaaacaata taaactgtca agccgccggc aacgtgctat aatgccgtgc atcttgcaca
    20581 aaccgaaaga ccgagcatta tgcgtatcgt agaaaaagcc tatactttcg acgatgtttt
    20641 gttggttccc gcacattcga ccgtgctgcc gcgagacgtt aaacttcaaa ccaagcttac
    20701 ccgcgaaatc acactcaacc tccccctgct ttccgccgcg atggataccg ttaccgaggc
    20761 gcgcctcgcc atttcgatgg cacaggaagg cggcatcggc atcatccata aaaacatgcc
    20821 gcccgaaatg caggcgcgcg ccgtttccaa agtaaaacgc cacgaaagcg gcgtggtcaa
    20881 agaccccgta accgttgcgc cgacaacgct catccgcgaa gtcttggaaa tgcgcgcgca
    20941 gcgcaaacgc aaaatgtccg gcctgcccgt cgttgaaaac ggcaaagtcg tcggcatcgt
    21001 aaccaaccgc gacctgcgtt ttgaaaaccg cgtcgatttg cccgtttccg ccattatgac
    21061 cccgcgcgaa cgtctggtta ccgtccccga aggcacaagc atagacgaag cgcgcgaact
    21121 gatgcacacg cacaaagtcg aacgcgtttt ggtcctgaac gaaaaagacg agcttaaagg
    21181 cttgattacc gttaaagata ttttaaaaac caccgagttt cccaatgcca acaaagactc
    21241 cgaaggccgt ctgcgcgtcg gtgcggcagt cggcaccggc ggcgacaccg acgagcgcgt
    21301 caaagccttg gtcgaagccg gcgcggacgt gattgtcgtc gatactgccc acggtcatag
    21361 ccaaggcgtg atcgaccgcg tgcgttgggt caaagaaacc tatccgcaca tccaagtcat
    21421 cggcggcaac atcgccactg ccaaagccgc attggatttg gtcaccgtcg gcgcggatgc
    21481 cgtcaaagtc ggtatcggtc cgggatcgat ttgcaccacc cgtatcgtgg caggcgtggg
    21541 cgtgccgcaa ctgaccgcca ttcacaacgt tgccgaagcc ctcaaaggca cgggcgttcc
    21601 gctgattgcc gacggcggca tccgcttctc cggcgacatc gccaaagccc tcgccgcagg
    21661 cgcgtacagc gtcatgctcg gcggtatgtt tgccggcacg gaagaagccc cgggcgaaat
    21721 cgaactctac caaggccgct cgtacaaatc ctatcgcggt atgggttcct tgggcgcgat
    21781 gagccaaggt tctgccgacc gctacttcca agacaaaacc gacagcaccg acaaatacgt
    21841 ccccgaaggc atcgaaggcc gcgttcccta caaaggcccg attgtgaaca tcatccacca
    21901 actgaccggc ggactgcgtt ccagtatggg ttatttgggt tgcgccaata ttgccgaaat
    21961 gcacgaaaaa gccgaatttg tggaaatcac ttccgcaggt atgagcgaat cgcacgttca
    22021 cgacgttcaa attaccaaag aagcgccgaa ttaccaccgc tgatttgaac agcctttcag
    22081 gacaaatgcc gtctgaaacc taatttccga gtttcagacg gcatttttgt tcgttcaagg
    22141 caagccgcac gggctgattg cgcggacggg caaagccatt ctgacggaca cgccgaaaga
    22201 atgaaaatgc cgtctgaacg ccgtttgcac cccgtctgcc gaataccgtc ggcctttaaa
    22261 acggcggtgg aaataaacgc aatctttcaa tacgggcgtt tcgattggtc ggaaatgatg
    22321 cgccggccgc catatcaaaa aatcg
//
LOCUS       DAWTQS010000030        21811 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.030, whole genome shotgun sequence.
ACCESSION   DAWTQS010000030 DAWTQS010000000
VERSION     DAWTQS010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21811
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.030"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(89..739)
                     /gene="pgmB"
                     /locus_tag="WNA25_001134"
     CDS             complement(89..739)
                     /gene="pgmB"
                     /locus_tag="WNA25_001134"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFA9823897.1"
                     /translation="MTFTAVLFDLDGVITDTAEYHYLAWKKLAEELGIGIDRKFNEQL
                     KGVSRDDSLKRILAHGGKTVGETEFAELTCRKNDNYLEMIQAVKPEDVYPGILPLLEA
                     LRANGKKIALASASKNGPFLLERMGLTHFFDTVADPAAVAHSKPSPDIFLAAAEGVGA
                     DIRRCRRRRHQSLWRFAHRRGQNRRLGQRHRAGVRHRRADLRLLAKCVGTVGQIKF"
     gene            complement(752..3009)
                     /locus_tag="WNA25_001135"
                     /pseudo
     CDS             complement(752..3009)
                     /locus_tag="WNA25_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234550.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
     gene            complement(3256..4217)
                     /locus_tag="WNA25_001136"
                     /pseudo
     CDS             complement(3256..4217)
                     /locus_tag="WNA25_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
     gene            complement(4221..4625)
                     /locus_tag="WNA25_001137"
     CDS             complement(4221..4625)
                     /locus_tag="WNA25_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HFA9823898.1"
                     /translation="MICSFILAKVPNKYHKAGYFGCLALGALGFFSIFFIYNQYALIL
                     SYILIGIAWAGIITYPLTIVANALSGKHMDTYLGLFNGSVCMPQIVASLLSFVLFPML
                     GGHQATMFLVAGAVLLLGAFSVCLIKETHGGV"
     gene            complement(4638..5657)
                     /locus_tag="WNA25_001138"
     CDS             complement(4638..5657)
                     /locus_tag="WNA25_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFA9823899.1"
                     /translation="MIGDRRAGNHFGFSKANTFQIKKKDLLYVGIYASNSKTRFARAG
                     KKHDLDVELRLSRRSDGLYPAKLADEPHFSNARRRPAQFGLVFHPAAAGGDAGSADSG
                     YYSDRTWKPRLGGRRLPYLLYGTLIAVIVMILMPNSGSFGFGYASLAALSFGALMIAL
                     LDVSSNMAMQPFKMMVGDMVNEEQKSYAYGIQSFLANTDAVVAAILPFVFAYIGLANT
                     AEKGVVPQTVVVAFYVGAALLIITSAFTISKVKEYDPETYARYHGIDVAANQEKANWF
                     ELLKTAPKVFWTVTPVQFFCWFAFRYMWTYSAGAIAENVWHTTDASSVGHQEAGNRYG
                     VLAAV"
     gene            5811..7487
                     /gene="ettA"
                     /locus_tag="WNA25_001139"
     CDS             5811..7487
                     /gene="ettA"
                     /locus_tag="WNA25_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFA9823900.1"
                     /translation="MSQQYVYSMLRVSKVVPPQKTIIKDISLSFFPGAKIGLLGLNGT
                     GKSTVLRIMAGVDKEFEGEAVPMGDIKIGYLPQEPELDPEKTVREEVESGFGEVAAAQ
                     KRLEEVYAEYANPDADFDALAEEQGRLEAIIAAGSSTGGGAEHELEIAADALRLPDWD
                     AKIGNLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESVEWLEQFLVRFPGTVVAV
                     THDRYFLDNAAEWILELDRGHGIPWKGNYSSWLEQKEKRLENEAKSEAARVKAMKQEL
                     EWVRQNAKGRQAKPKARLARFEEMSNYEYQKRNETQEIFIPVAERLGNEVIEFVNVSK
                     SFGDKVLIDDLSFKVPAGAIVGIIGPNGAGKSTLFKMIAGKEQPDSGEVKIGQTVKMS
                     LIDQSREGLQNDKTVFDNIAEGRDILQVGQFEIPARQYLGRFNFKGSDQSKIARQLSG
                     GERGRLHLAKTLLGGGNVLLLDEPSNDLDVETLRALEDALLEFAGSVMVISHDRWFLD
                     RIATHILACEGDSKWVFFDGNYQEYEADKKRRLGEEGAKPKRIKYKPVTR"
     gene            complement(7547..8032)
                     /locus_tag="WNA25_001140"
     CDS             complement(7547..8032)
                     /locus_tag="WNA25_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFA9823901.1"
                     /translation="MADFKPDFAWLLKRPLCFLAFGFGSGLAPFAPGAFGTLAALPLA
                     FVLILLGIDGLLLAFVCIVLFMWGIRICACAERETGVSDHGGIVWDEIVAMLFVLAFV
                     PFRWTWWLAAFVLFRLFDALKPPPVGWFDKNLHGGLGIMADDMAAAVMTLIVLRIAML
                     F"
     gene            complement(8025..8981)
                     /gene="thiL"
                     /locus_tag="WNA25_001141"
     CDS             complement(8025..8981)
                     /gene="thiL"
                     /locus_tag="WNA25_001141"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFA9823902.1"
                     /translation="MKEFDFIKRYLQTGTDKDVVLGIGDDAAIVRPREGFDLCFSADM
                     LLKDRHFFADVKPEGLAWKVLAVNISDMAAMGAIPRWVLLSAALPELDEVWLERFCGS
                     FFGLAKKFGVTLIGGDTTKGDMAFNVTIIGELPKGRALRRDAAVAGDDIWVSGRVGMA
                     AAALNCRLKRCVLPDDVFAECEQKLLRPEPRVGLGLALLPFARAAQDVSDGLAQDLGH
                     ILTASGVGAEIWADSLPSLSVLKDILPRAQWLSYTLAGGDDYELVFTAPESCRSRVLD
                     AAERCGVPVTCIGKINGGCRLKVLDAGGRELELHSLGFDHFG"
     gene            complement(9756..10466)
                     /gene="rmpM"
                     /locus_tag="WNA25_001142"
     CDS             complement(9756..10466)
                     /gene="rmpM"
                     /locus_tag="WNA25_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding outer membrane protein
                     RmpM"
                     /protein_id="HFA9823903.1"
                     /translation="MTKQLKLSALFVALLASGTAVAGEASVQGYTVSGQSNEIVRNNY
                     GECWKNAYFDKASQGRVECGDAVAVPEPEPAPVAVVEQAPQYVDETISLSAKTLFGFD
                     KDSLRAEAQDNLKVLAQRLSRTNVQSVRVEGHTDFMGSEKYNQALSERRAYVVANNLV
                     SNGVPASRISAVGLGESQAQMTQVCQAEVAKLGAKASKAKKREALIACIEPDRRVDVK
                     IRSIVTRQVVPARNHHQH"
     gene            10830..11780
                     /locus_tag="WNA25_001143"
     CDS             10830..11780
                     /locus_tag="WNA25_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HFA9823904.1"
                     /translation="MKLQQLKYALEVYRHNLNVSEAAEALFTSQPGISKQIKLLEEEI
                     GIQIFIRSGKRVVSVSQPGKVVLDIAERILRDVQNIKNIGSEFTGQDSGSLTVATTHT
                     QARYALPLIVADFVKRYPKVNLTIKQGSPAAIAQMVTSGESDLAIVTERIDDHPELGR
                     LSCYDWTHAVIVPNDHPLLECRNPLRIEDLARFPLITYEFAFNAGSSIARAFAKARLE
                     RPDVALAAADTDVLKTYVRLGLGVGLMAKMAYNPDTDGDLQLVDAAHLFEPSPTWIAL
                     RSDTYLRGYAYDFIQAFAPHLTREKVDRILYTPISEDFSI"
     gene            complement(11893..12627)
                     /gene="fnr"
                     /locus_tag="WNA25_001144"
     CDS             complement(11893..12627)
                     /gene="fnr"
                     /locus_tag="WNA25_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFA9823905.1"
                     /translation="MASHNTTHQMKTLCSSCSLRELCLPVGLLPNEFSQLDAVIRQSR
                     RLKKGEYLFRAGGAFTSLFAIRSGFFKTTVASQDGRDQVTGFFMSGELIGMDGICSYV
                     HSCDAVALENSEVCELPFTHIEELGQNIPSLRTHFFRMISREIVRDQGVMLLLGNMRA
                     EERIAAFLLNLSQRLYSRGFAANDFILRMSREEIGSYLGLKLETVSRTLSKFHQEGLI
                     SVEHKHIKILNLQVLKKMVSGCSHAI"
     gene            12762..14183
                     /gene="hemN"
                     /locus_tag="WNA25_001145"
     CDS             12762..14183
                     /gene="hemN"
                     /locus_tag="WNA25_001145"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFA9823906.1"
                     /translation="MKIIQIQNNHNVNDDRPEFDRALIASLPASGPRYTSYPTADRFH
                     DGFREGEYIKVLHLRGMGALNKPLSLYIHIPFCNTICYYCGCNKIITKDKSRADTYIE
                     YLEKEMELLAPHLNGRHQLAQLHFGGGTPTFLSDEQIERVFRMIRKHFELIPSGEYSI
                     EIDPRKVSRDTVLMLGRLGFNRMSVGIQDFDPKVQAAVNRIQSYEETKEVIDAAREAG
                     FKSVSVDLIYGLPHQTSESIKTTIDTVLSLDPDRLALYHYAHLPHVFKPQRRIDTAAV
                     PDSEEKLDMLQYCVQTLTERGYVFIGMDHFAKPDDELSIALKEGFLQRNFQGYSTYAD
                     CDLVAIGVSSIGKIGSTYSQNERDIDAYYAAIDEGRLPIMRGYQLNQDDILRRNIIQD
                     LMCRFALDYRIYESMFGIPFDRYFKDELADLEKLAGLGLVRLNSHGLTVTPKGRFLIR
                     NIAMVFDYHLRHKETKAKYSQTV"
     gene            complement(14221..14409)
                     /locus_tag="WNA25_001146"
     CDS             complement(14221..14409)
                     /locus_tag="WNA25_001146"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823907.1"
                     /translation="MPSEPVFRRHFVDGMCGLVPYAQSVLSGYAVWYLGFGTVGHKGY
                     DMPPPVLENTYPRQQNAV"
     gene            complement(14423..15997)
                     /locus_tag="WNA25_001147"
     CDS             complement(14423..15997)
                     /locus_tag="WNA25_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HFA9823908.1"
                     /translation="MAQIQMSANVKTINAVFAAMLVGTVGYFIYWGLGYTHYNYAALF
                     IIATMFGVFMAFNIGGNDVANSFGTSVGAGTLTIPQALLIAAVFEVSGAVIAGGEVTD
                     TIRKGIVDLKGVDFEPIQFVFIMMSALLAAALWLLFASRKGLPVSTTHAIIGGIVGSA
                     LCMAFTHNADGVALIRWGKLGEIGMSWVLSPVLGGAVSYFLFSRVKKNVLDYNAWAEG
                     TLKGIKQEKKAYKERHRLFFEGLSEAEKVEYATKMAHDAQIYDEPEFDPQELQSEYYR
                     GLYAFDNRKNNVDSYKALHSWIPFIASFGTMMISAMLIFKGLKNLHLGMSNVNSFLTI
                     FMIGAAVWMGTFVFAKSLKRKDLGKSTFQMFSWMQVFTACGFAFSHGANDIANAIGPF
                     AAIMDVLRTNSVAAQSAVPPIAMLTFGIALIVGLWFVGKEVIKTVGTSLAEMHPASGF
                     TAELSAASVVMGASLMGLPVSSTHILVGAVLGIGLVNRNANWKLMKPIGLAWVITLPA
                     AAVLSMASYLILQAVF"
     gene            16322..17422
                     /locus_tag="WNA25_001148"
     CDS             16322..17422
                     /locus_tag="WNA25_001148"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFA9823909.1"
                     /translation="METQLYIGIMSGTSMDGADAVLVRMDGGKWLGAEGHAFTPYPDR
                     LRRKLLDLQDTGTDELHRSRMLSQELSRLYAQTAAELLCSQNLAPCDITALGCHGQTV
                     RHAPEHGYSIQLADLPLLAELTRIFTVGDFRSRDLAAGGQGAPLVPAFHEALFRDDRE
                     TRVVLNIGGIANISVLPPGAPAFGFDTGPGNMLMDAWTQAHWQLPYDKNGAKAAQGNI
                     LPQLLGRLLAHPYFSQPHPKSTGRELFALNWLETYLDGGENRYDVLRTLSRFTAQTVC
                     DAVSHAAADARQMYICGGGIRNPVLMADLAECFGTRVSLHSTAELNLDPQWVEAAAFA
                     WLAACWINRIPGSPHKATGASKPCILGAGYYY"
     gene            17541..18395
                     /locus_tag="WNA25_001149"
     CDS             17541..18395
                     /locus_tag="WNA25_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HFA9823910.1"
                     /translation="MKTLLLLIPLVLTACGTLTGIPALKGRKAALYVSVMGDQGSGNI
                     SGGRYSIDALIRGGYHNNPDSATRYSYPAYDTTATTKSDALSGVTTSTSVLNAPAAAL
                     TKNNGRKGERSAGLSVNGTGDYRNETLLANPRDVSFLTNLIQTVFYLRGIEVVPPEYA
                     DTDVFVTVDVFGTVRSRTELHLYNAETLKAQTKLEYFAVDRDSRKLLIAPKTAAYESQ
                     YQEQYALWMGPYSVGKTVKASDRLMVDFSDITAYGDTTAQNRPDFKQNNGKNPDVGNE
                     VIRRRKGG"
     gene            18399..20129
                     /gene="mafB"
                     /locus_tag="WNA25_001150"
     CDS             18399..20129
                     /gene="mafB"
                     /locus_tag="WNA25_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HFA9823911.1"
                     /translation="MKPLRRLTNLLAACAVAAAALIQPALAADLAQDPFITDNTQRQH
                     YEPGGKYHLFGDPRGSVSDRTGKINVIQDYTHQMGNLLIQQAAIQGNLGYTVRFSGHG
                     HEEHAPFDNHAADSASEEKGNVDDGFTVYRLNWEGHEHHPADAYDGPKGGNYPKPTGA
                     RDEYTYHVNGTARSIKLNPTDTRSIRQRISDNYNNLGSNFSDRADEANRKMFEHNAKL
                     DRRGNSMEFVNGVAAGALNPFISAGEALGIGDILYGTGYAIDKAAMRNIAPLPAEGKF
                     AVIGGLGSAAGFEKNTREAVDRWIQENPNAAETVEAVFNVAAAAKVAKLAKAAKPGKA
                     AVSGDFSKSYTCSFHGSTLVRTADGYKAIAHIQAGDRVLSKDEASGETGYKPVTARYG
                     NPYQETVYIKVSDGIGNSQTLISNRIHPFYSDGKWIKAEDLKAGSRLFAENGAEQTVQ
                     SVTVKPEPLKAYNLTVADWHTYFVKGSQAETEGVWVHNDCPPKPKPTNHAQQRKEEAK
                     NDSHRSVGDSNRVVREGKQYLDSDTGNHVYVKGDKVVILTPDGRQVTQFKNSKANTSK
                     RVKNGKWTPK"
     gene            20107..20388
                     /locus_tag="WNA25_001151"
     CDS             20107..20388
                     /locus_tag="WNA25_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823912.1"
                     /translation="MGNGHQNNQSSLEKRIFYLEHSGQYLMICALSDYSQNKHTVVMA
                     NFLYPNEKMDWRNLDDLFNELVLEELQSSFMDWYPTIEKAISHHLEDFS"
     gene            20444..21091
                     /locus_tag="WNA25_001152"
                     /pseudo
     CDS             20444..21091
                     /locus_tag="WNA25_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147002.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            21088..21504
                     /locus_tag="WNA25_001153"
     CDS             21088..21504
                     /locus_tag="WNA25_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823913.1"
                     /translation="MKTSTIVFGGFFITDNGERIQIPILENPNIKEINNFFSVSNFEK
                     KAGVLVFRIIPEPEFGNTELTIYFEKGYYLPIIQTILEDGDIEVKNLKTENYSGNTME
                     ILGDVYPIEHISKNISIIQDIISEFIMKNKPITIMI"
     gene            21607..21811
                     /locus_tag="WNA25_001154"
                     /pseudo
     CDS             21607..21811
                     /locus_tag="WNA25_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951255.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
BASE COUNT         5600 a         6039 c         5495 g         4677 t
ORIGIN      
        1 gggatttttg caaaggtctc gggttatcat tctgttctaa aagcagaaag gccgtctgaa
       61 acactgttca gacggccttt ttacttcctt agaattttat ctgcccgact gttcccacac
      121 attttgcaag taggcgtagg tcagctcggc ggtgtcggac accagcgcga tgtcgctgcc
      181 caagtcttcg gttttgccca cgccgatggg caaagcgcca gaggctttga tggcggcgac
      241 gccggcagcg gcgtatgtcc gcacccacgc cctcggcggc ggcaaggaag atgtcggggg
      301 agggtttgga atgcgcgacg gcggcagggt cggcgacggt gtcgaagaag tgggtcagcc
      361 ccatgcgttc gagcaagaac gggccgtttt tactggcgga cgcgagggcg atttttttgc
      421 cgtttgccct caatgcttcc agcagcggca aaatgccggg gtacacgtct tcgggtttga
      481 ctgcctgaat catctctaag tagttgtcgt ttttgcggca ggtcagttcg gcgaactcgg
      541 tttcgccgac ggttttgccg ccgtgcgcga ggatgcgttt gagcgaatcg tcgcgcgaca
      601 cgcctttgag ctgctcgtta aacttgcggt cgatgccgat gcccaattct tcggcaagct
      661 ttttccatgc gaggtagtgg tattcggcgg tatcggtaat cacgccgtcg agatcgaaga
      721 gcactgcggt aaaagtcatt ttgcgccctc cttatttttc caatgcaacg gtgtggctgc
      781 cgttgagcgt gatatctttg ccgtacacct gcaattcgag cggatcgcct ttgagcaggg
      841 tgaagacgac gttttttgcc gacggcgact ttaatcagac ggccgcggta gttgatgtgg
      901 aaggcatagc ctgtccacgc actcggcagg aacggtgcga agctgagttt gccgccccaa
      961 gttttcattt gggcgaaacc ttggacgatg gcgagccacg agccggtcat agaagtaata
     1021 tgcaggccgt cttcggtgtc gttgttgtag tcgtccaagt ccaggcgggc ggtgcgccgg
     1081 tacatttcca cggctttttc ttctttgccc agttcggcag cgaggatgga gtggatacat
     1141 ggagacaacg agctttcatg cacggtcatc ggttcgtaga agtcgaagtt gcggcgtttt
     1201 tcgtccatgt cgaaacggtc gccgaagaag tagatgcctt gcaatacgtc cgcctgtttg
     1261 atgaagggcg aacgcaggat tttgtcccac gaccatttct ggttgagcgg caaatcgtcg
     1321 ggcgaaagcg cggatacggg gcggatgtct ttgtcaagga agccgtcgtg ctgcacgaat
     1381 acgccgagtt cttcgtcatg cggacggtac atattcgcgc tgatgtccgc ccatttttcc
     1441 aactcggcgg cgctcacgtt cgaacccgga cgcgggtatt tcgccaaggc ttcgcgggtg
     1501 tagcccaata cccatgcggc gagggtgttg gtttaccagt tgttgttgat gttgttttcg
     1561 tattcgttcg gaccggttac gccttgaatc atgtatttgc cgttgcgttt ggagaagtgg
     1621 acgcggtccg cccggaagcg ggacacttcg accaagactt ccaagccttc tttggcaaga
     1681 tagcactcgt caccggtgta gttggtgtag ttgtagatgg cgtaaggaat cgcgccgtta
     1741 cggtggattt cctcgaaggt gatttcccat tcgttgtggc actcgatgcc cgtaaacgtt
     1801 accatcggat agagtgcgcc cgccaagccc tgttcgcgtg cgttatgctg cacctgcggc
     1861 aatttggttg cggcggtatt gcagcaggtt gcgggtaact tcgggttccg ccagtgcaag
     1921 gtagagcggc acggcgtagg cttcggtgtc ccaataggtc gcgccgccgt atttttcgcc
     1981 ggtaaagcct ttggggccga tgttcagacg cgcgtcttcg ccgtagtagg tggagaacag
     2041 ttggaacagg ttgaagcgga tgccctgctg tgcttcgtcg ctgccttcga tgaccacgtc
     2101 ggcgatttcc caacgatgct gccagcctgc tttgtgcgca tccagcaagg tttcaaacgc
     2161 aacacctgcg attttttcag ataaggcgcg gccggcggct ttcactgctt ccaagccctg
     2221 ataatcgcgg ctggtggtaa caatcacgcg tttttcaaag gtttcgggcg tgccgccgac
     2281 ttcggcttca aaagaattag agacctgcca atcggtttgg ctgccgccga aggctttgaa
     2341 gctgccggcg aaggtttgcc cggcgttgac gataaattgt tccacaccga aagggttggc
     2401 gacggtttgg gcggcaatgt aggagcggtc gtctgaaacg cctttgtcca atacctgcca
     2461 gaatttttct tcgtagttgg agtcttcgtt tttcacgtcg gcatcgatga tggaatcgat
     2521 gcggacttgg tgggttttgc cgtcaacgga cacggcttcc cagcgggtga gagccagctc
     2581 tttttgcgcg accgacagga atttgcacac atcgaaacgc acaccgaata cggtgaacga
     2641 gcggcgcaac acgccgtgtt gcatatccag ttcgacggag aagccggcaa cgtcgttttt
     2701 cgccaagtcc acctcttgcc cgtcgacaaa gattttgact ttgctgaaat tgagcgcgtt
     2761 gatggctttg ccgaaatatt tgggatagcc gtttttccac cagccgacgc gggttttgtc
     2821 gggaaaccac acgccggcga tgtaggtgcc caagtggctg tcggcggagt aggtttcctc
     2881 aaagctgccg cgcataccca tatagccgtt gcccaagctg gtcaggctct cttgcagccg
     2941 tttgtgttct ttttccagtt ttgccgaacg cagcgtccaa gggctgattt ccataattct
     3001 tgtgtacatc gtaaagctcc tgtttggatt gatttgaggg aatggtgaaa tcttatagtg
     3061 gattaacaaa aatcaggaca aggcgacgaa gccgcagaca gtacagatag tacggcaagg
     3121 cgtggattaa caaaaatcag gacaaggcga cgaagccgca gacagtacag atagtacggc
     3181 aaggcgaggc aacgccgtac tggtttttgt taatccacta taaaaaggtc gtctgaaacc
     3241 ggtgttagga agctcctaag aaagggattc gatgccgtaa gcaatcgtcg cctccctagt
     3301 atcacccttg ttcagacgga tattgccgaa ttcgggccaa tgcaggctgt cgggcagcgt
     3361 ctgcgcctcg gtcgccagcg cgtcgtaaac gcccgcatcg tgccgcgcga aatcctgcgg
     3421 ggcggcggta aagatgacca agccgttgcg gtcgctgtat atgctgatac gacggcagcg
     3481 tccggcttgc aacacggcgg cgggacggcc tatatcggac ggcacgcggt aagcgtcgtc
     3541 aaaaccggcc cgtttcgcgg cgcagggcgg caacggcggc atccggcggc ttgggccggc
     3601 tgaaatcaaa tacttcgagg ccgtctgaaa ccgttaagac gggcagtttt tcggcatcgg
     3661 ccggaatatg tccgccctgc ggaatatgca gaaccgcatc gtgcaggccc gcgtccagcc
     3721 gccagtaaat gtgcagcgtc gggtcgaaca ccgtgtcgcc gagcgcggtg gcgcgatagg
     3781 taacggtaag ccggccatcc tcgtccaagc ggtaggaaat atccaaatcg ttgggataac
     3841 cgtcggccgt ctgccgccac cgcgttgaaa cgggtaacgg ccagcccgtg cgaaccgccg
     3901 tgcagcgcgt tcctgccttc gttggcctcc acgcggtaag tcctaccgtt gatgtcgaac
     3961 gccgcaccgc ggatgcgtcc ggccacgcgc cctatctgct tgttaatctg aaacggattg
     4021 tccgcatagg aagccgcatc gtcgaacgac accacggggt tttcgcgcac gccgtctgcc
     4081 aaaacggaaa attcctgcac aatcccgccc aagtccagca cgaagacgca cgtaccacgc
     4141 cggttggaca gcacatagcc ggttacggcc cgcccgtcga tcaggccgaa atcgcgggta
     4201 gcgggggtat cgctcatcgc tcaaaccccg ccgtgggtct ctttaatcag acagactgag
     4261 aaggctccca gcagcaagac tgcgcctgca accaagaaca tggttgcctg atggccgccc
     4321 agcatcggga aaagcacgaa actcaacagc gaagcgacga tttgcggcat acagacagag
     4381 ccgttaaaca ggcccaaata agtatccatg tgtttgcccg acaaagcgtt ggccacaatc
     4441 gtcagcggat aagtgataat gcccgcccaa gcgatgccga ttaagatata agacaggatg
     4501 agtgcgtatt gattgtagat gaagaagata gagaagaaac cgagcgcgcc caaagccaaa
     4561 cagccgaaat aacccgcctt atggtattta ttcggtactt ttgccagaat aaacgaacaa
     4621 atcaccgccg caaccgacta caccgccgcc aaaacgccgt accggttgcc cgcctcctga
     4681 tggcctacgg aagacgcatc ggtagtgtgc cagacgtttt ctgcaatcgc gcctgccgag
     4741 taagtccaca tataccggaa ggcgaaccag cagaaaaact gtaccggagt aaccgtccaa
     4801 aacactttag gcgcggtttt taagagttcg aaccagttgg ctttttcctg attcgcggcg
     4861 acatcgatgc cgtggtaacg ggcgtaggtt tccgggtcgt attctttgac tttggagatt
     4921 gtgaacgcac tggtaataat cagtaacgcc gcacccacat agaatgctac gaccacggtt
     4981 tgtggcacaa cgcctttctc ggcagtgttc gccaaaccga tatacgcgaa cacaaacggc
     5041 agaatcgctg ccacaaccgc gtccgtattc gctaagaaac tttgaatccc gtaggcgtag
     5101 cttttctgct cctcgttgac catatcgccg accatcatct taaacggctg catcgccata
     5161 ttcgacgaca cgtccaacag cgcaatcatc agcgcgccga acgacaaggc cgccagcgac
     5221 gcatagccga aaccgaagct gcccgagttc ggcatcaaaa tcatcacgat gaccgcaatc
     5281 agcgtgccgt aaagcagata cggcaggcgg cggccgccca agcgcggctt ccaagtgcgg
     5341 tctgagtagt agccactatc ggctgaacca gcatccccgc cagcggcggc aggatgaaaa
     5401 accagcccaa attgtgcggg tctgcgccta gcgtttgaaa aatgcggctc atctgcgagc
     5461 tttgcagggt aaaggccgtc tgaacgccga gatagccgaa gctcaacatc caaatcgtgc
     5521 tttttgccgg cgcgggcaaa ccttgttttg ctgtttgagg cgtatattcc gacataaagt
     5581 aaatcctttt ttttgatttg aaaagtattt gctttggaaa atccgaaatg gttgccggcg
     5641 cggcgatccc ctatcattat tatttttttg tctgtataat ttcaaaggga taggcggatt
     5701 ttatgaatcc tgcccgattt tggcaatacc ggttcgcgga taaactggct taaatcaaat
     5761 tatcggttaa aatggccgtc tgaaatttgt ttgaccgaat cgagaaaact atgtcccaac
     5821 aatacgtcta ttctatgctg cgcgtgagca aggttgtgcc gccgcagaaa accatcatta
     5881 aagatatttc cctttctttc ttccccggcg cgaaaatcgg cctcttgggc ttgaacggca
     5941 cgggcaagtc caccgtgctg cggattatgg cgggcgtgga taaagaattt gagggcgaag
     6001 ccgtgccgat gggcgacatc aaaatcggct atctgccgca agagccggag cttgatccgg
     6061 aaaaaaccgt gcgcgaggaa gtggaaagcg gtttcggcga agtggctgcc gcacagaaac
     6121 gtttggaaga agtgtatgcc gagtacgcca atcccgatgc ggattttgac gcgctggcgg
     6181 aagaacaagg ccgcttggaa gcgattatcg cggcgggttc gtcaactggc ggcggtgcgg
     6241 aacacgaatt ggaaatcgct gccgacgcgc tgcgcctgcc tgattgggat gccaaaatcg
     6301 gcaatctttc gggcggtgaa aaacgccgcg tcgctttgtg caaacttttg ttgagcaagc
     6361 ccgatatgct gctcttggac gagccgacca accacttgga tgcggaatcg gtggaatggc
     6421 tggagcaatt cctcgtgcgc ttccccggca cagtggtcgc ggtaacgcac gaccgctact
     6481 tcctcgacaa cgccgccgaa tggattttgg aactcgaccg cggacacggc attccgtgga
     6541 aaggcaatta ctcgtcttgg ctggagcaga aagaaaaacg cttggaaaac gaggcgaaat
     6601 ccgaagccgc gcgcgtgaag gcgatgaagc aggaattgga atgggtgcgc caaaatgcca
     6661 aaggccgcca agccaagccc aaagcgcgtt tggcgcgttt tgaagaaatg agcaactacg
     6721 aataccaaaa acgcaacgaa actcaggaaa tctttatccc tgttgccgag cgtttgggta
     6781 acgaagtgat tgaatttgtg aatgtttcca aatcgttcgg cgataaagtg ctgattgacg
     6841 atttgagctt caaagtgccg gcgggcgcga ttgtcggcat catcggcccg aacggcgcgg
     6901 gtaaatcgac gctgttcaaa atgattgcgg gcaaagagca gcccgattcg ggcgaagtga
     6961 aaatcgggca aaccgtgaaa atgagcttga ttgaccaaag ccgcgaaggt ttgcaaaacg
     7021 acaaaaccgt gttcgacaac attgccgaag gtcgcgatat tttgcaggtc ggacagtttg
     7081 aaatccccgc ccgccaatat ttgggacgct tcaactttaa aggcagcgac caaagcaaaa
     7141 tcgcaaggca gctttccggc ggcgaacgcg gccgtctgca cttggcaaaa accttgttgg
     7201 gcggcggcaa tgtgttgctg ctggacgaac cgtccaacga tctcgacgtg gaaaccctgc
     7261 gcgcgttgga agacgcattg ttggaatttg ccggcagcgt gatggtgatt tcgcacgacc
     7321 gctggtttct cgaccgcata gccacgcata tcttggcgtg tgaaggcgac tccaaatggg
     7381 tgttcttcga cggcaactat caagaatacg aagccgacaa gaaacgccga ctcggcgaag
     7441 aaggcgcgaa accgaaacgc atcaaataca aaccggtaac gcgttaacct ccgaaacaat
     7501 gccgtctgaa aggctttcag acggcatttt tacaaggcag ccccgtttaa aacagcattg
     7561 caatcctcaa gacaatcaag gtcatcaccg cagccgccat atcgtccgcc ataatgccca
     7621 aaccgccgtg cagattcttg tcaaaccaac cgacgggggg cggtttgagc gcatcaaaca
     7681 gacggaacag aacaaatgcc gccagccacc acgtccacct gaacggcaca aacgccagca
     7741 caaacagcat ggcgacaatc tcgtcccaaa caatcccacc gtggtcactg acacccgttt
     7801 cacgttccgc acacgcacaa atacgtatgc cccacataaa cagcacgata cacacaaaag
     7861 ccagcagcag cccgtctatg ccgagcaaaa tcagcacaaa cgccaaaggc agtgccgcca
     7921 aagtgccgaa tgcgcccggc gcaaacggag ccagcccgct gccgaaaccg aaagccaaaa
     7981 aacacaacgg ccgtttcaac agccacgcaa agtcaggttt aaaatcagcc aaaatgatcg
     8041 aatcctaaag aatgtagttc caattccctg ccgccggcat ctaaaacctt cagacggcat
     8101 cctccgttga ttttgccgat gcacgttacc ggcacgccgc accgttccgc cgcatcaagc
     8161 acgcggctgc ggcaactttc cggagcggta aaaaccagct cgtaatcgtc gccgcccgcc
     8221 aaagtataag acagccattg cgctcggggc aaaatatctt tcaatacaga taaagatggc
     8281 agcgaatcgg cccaaatttc cgcccccacg ccggaagcgg tcaggatatg ccccaaatct
     8341 tgcgcaaggc cgtctgaaac atcctgtgcc gcccgggcaa acggcaacag cgcaagcccc
     8401 agcccaaccc ttggctcggg acggagcagc ttttgttcgc attcggcgaa cacatcatcc
     8461 ggcaacacac accgtttcag acggcagttc aaagccgccg ccgccatacc gacacgcccc
     8521 gacacccaaa tatcgtcgcc cgcaaccgcc gcatcacgcc gcaacgccct acccttcggc
     8581 aattcgccga taatggttac attgaacgcc atatcgccct tggtcgtatc gccgccgatt
     8641 aacgttacgc caaacttttt tgccaaaccg aaaaagctgc cgcaaaaccg ttccagccat
     8701 acttcatcca attcgggcaa cgccgcgctc agcagcaccc aacgcggtat cgcacccatc
     8761 gccgccatat ctgaaatatt gacggccaaa accttccaag ccaagccttc aggtttgaca
     8821 tctgcaaaaa aatgcctgtc cttcaaaagc atatccgcac tgaaacacaa atcgaagcct
     8881 tcgcgcgggc ggacaatcgc cgcatcgtcg cctatgccca atacgacatc cttatccgta
     8941 cctgtttgca aataccgttt gatgaagtcg aattctttca tatcccaaaa tatccggcgt
     9001 gcgaatccaa tttaaatcat agcaaagcag caaaggtcgt acaaacaata aagcggctca
     9061 ttcagtgttt cagcgtattt cacttttagc tttagccata ccgtacccgt atgcaccgac
     9121 caccctgttt caacacacaa acggcatatc aaagcaccgc actgcacgtc gccccgaatg
     9181 caaagcggca acttccgcca ccgattaggc tcaatcgctg caactggcgg ggtttcaacc
     9241 gaaaagggat gatgataaaa ccatatccgg catacagtgc cgcacacatc gggcacgatt
     9301 ccatccattt cccaatgagg gggaaaggcg tgaatttgta tgaattatcc ctttcggggc
     9361 ttgatacaga aaggattccc ttgctatggg caaacccaca tcctaccgcc ttaaaatcag
     9421 agtttcaacc gatatagtgg attaaattta aaccggtacg gcgttgcctc gccttgccgt
     9481 actattgtac tgtctgcggc ttcgtcgcct tgtcctgatt tttgttaatc cactataaag
     9541 cgcggcaatg ggacacaaac atgaatctga atccgaatcc gaacctgcac cccgattctg
     9601 acgatagccg tttcagaaaa ccacccaaaa aaacaaaccc cgcaaaagcg gggtttgttt
     9661 cacatcatcg ataccggccg acgtttcacg aatattgcaa cagtacccgg tacaaaatcc
     9721 gctaacctca tgcatcggca agatattacc tagccttagt gttggtgatg attgcgtgcc
     9781 ggcacaacct gacgggttac gatgctgcgg attttcacat ctacgcggcg gtcaggttcg
     9841 atacatgcaa tcagagcctc acgttttttg gctttagagg ctttcgcacc cagtttggca
     9901 acttcggctt gacaaacttg agtcatttgc gcttgagatt cgcccaagcc gacagcagaa
     9961 attctagaag caggtacgcc gttgctgacc aggttgtttg ccactacgta tgcgcggcgt
    10021 tcggacagag cctgattgta tttttcagaa cccataaagt cggtatggcc ttcgacgcgg
    10081 acagattgga cattggttcg actcaggcgt tgcgccaata ctttcaggtt gtcttgagct
    10141 tcggcgcgca atgaatcctt atcgaaaccg aacagggttt tggcagacag ggaaatggtt
    10201 tcatcaacat attgaggagc ctgctccaca acggcgacag gcgcgggttc gggctcgggg
    10261 acggcaaccg catcgccgca ttctacgcga ccttggcttg ctttatcaaa gtaggcgttt
    10321 ttccagcatt ctccatagtt gttgcgtacg atttcgttcg actggccgct tacggtgtaa
    10381 ccctgaacgg acgcctcgcc cgcaacagca gtgccggaag cgagcaatgc aacgaataat
    10441 gcgcttaatt tcagctgttt ggtcatttta ttccctcatt agatttgtac agcagataca
    10501 gaagcaactg ctgaattgcc aacatacagg ccgctgtaga aacatggcgg caaatcggat
    10561 atttcagtct ccactataaa gaaacagggg gcggactgtc aatactcaac gcctgaaaac
    10621 aaccgtaaaa cagcctgttt cctgatagtc tatcatgccg aaaaatcaat tccgtgtcat
    10681 tactttaggg ggattttttc catatcgcac gattgccgtt gcacaaaaac aacaaacaca
    10741 acggcaaagc cccataccgt accggcaaga aaatatgata aattataaac aatgttcagc
    10801 cacccgacac agacccacac cgacccgcca tgaaattaca acaattgaaa tacgccttag
    10861 aagtttaccg gcacaacctg aacgtttccg aagcggccga agccttattc acatcacaac
    10921 ccggcatctc caaacaaatc aaattgctgg aagaagaaat cggcattcag atttttatcc
    10981 gcagcggcaa gcgcgtggtt tcggtctcgc agccgggcaa ggtggttttg gatattgcgg
    11041 aacgtatttt gcgcgatgta cagaacatta aaaatatcgg cagcgagttt accggacagg
    11101 acagcggttc gctgacggtt gccacgacgc atacgcaggc gcgctatgcc ctaccgttga
    11161 ttgttgccga ttttgtgaaa cgctatccga aagtcaatct gaccatcaaa cagggaagcc
    11221 ctgccgccat cgcccaaatg gttacttcgg gagaatctga tttggcgatt gttacggaac
    11281 ggatagacga ccaccccgag ttgggaaggc tttcctgcta cgactggaca cacgcggtga
    11341 ttgtgccgaa cgaccatccg ctgctcgaat gcaggaatcc cctccgtatt gaagatttgg
    11401 cgaggtttcc gctgattact tatgaatttg cattcaatgc gggcagcagc atcgcgcggg
    11461 cattcgccaa agcccgtttg gaacggccgg atgtcgcatt ggcggcggcg gatacggatg
    11521 tattgaagac ttatgtgcgc ttgggtttgg gcgtgggact gatggcgaaa atggcataca
    11581 acccggatac ggacggcgat ttgcagcttg tggatgcggc acacctgttc gagccgtcgc
    11641 cgacgtggat agctttgcgc agcgatactt atttgcgcgg atatgcctac gactttatcc
    11701 aagcgtttgc gccgcacctg acacgcgaga aggtggatag gattctgtac acgcccatca
    11761 gcgaggattt ttcgatttag gcggctgccg gttttcaggc ggcactttgc ggcagataca
    11821 acaaacaggg cagatgtttt cgtctgccct gtgtttattg agaatgccgt ctgaaatgtt
    11881 cgtacaggtt aatcaaatgg cgtgcgaaca accggatacc atttttttca gcacctgcag
    11941 attgagaatc ttgatgtgtt tgtgttcgac ggaaatcaat ccttcctgat ggaacttgga
    12001 taatgtgcgg ctgacggttt caagtttcag cccgagataa ctgccgattt cttcgcggga
    12061 cattcttaag atgaagtcgt tggcggcgaa acctcgggaa taaaggcgtt gggaaaggtt
    12121 caacaggaag gcggcaatcc gctcttcggc gcgcatattg cccaacaaca gcataacacc
    12181 ttgatcgcgc acaatttcgc ggcttatcat gcggaaaaag tgtgtacgca ggctggggat
    12241 attctgaccg agctcttcga tatgggtaaa cggcaactcg catacctcgc tgttttccaa
    12301 ggcgaccgcg tcgcaactgt gcacatagga acagatgccg tccatgccga tgagttcgcc
    12361 cgacataaag aaacccgtta cctgatcgcg gccgtcctga ctggcgacgg ttgttttgaa
    12421 gaagcccgaa cggatggcaa agagcgaggt aaaggctccg ccggcacgga acaggtattc
    12481 gccttttttc aggcggcggc tttggcggat gacggcatcg agttggctga actcgttggg
    12541 cagcagcccg acaggcaggc agagttcccg caaagaacag gaagaacaca gcgttttcat
    12601 ctgatgtgta gtattatgcg aagccatacc gtaccttttc gtgtgttttg ccccatcccg
    12661 attatgtgct tgttgacaca tatcaagaca ggtttatcat actgtggcat tctaccaaac
    12721 tatccaaaat ttgactaaca tcataaaacc gcacacacac catgaaaatc attcagatac
    12781 agaacaatca caatgtaaac gatgaccgcc ccgagtttga ccgcgcgctg attgccagcc
    12841 tgcccgccag cggtccgcgc tatacttcct accctaccgc cgaccgtttc catgacggtt
    12901 tccgcgaagg cgaatatatc aaagttttac atttgcgcgg tatgggcgcg ttaaacaaac
    12961 cgctttccct ttacattcac attccgtttt gcaacaccat ctgctactac tgcggctgca
    13021 acaaaatcat caccaaagac aaaagccgcg ccgataccta catcgaatat cttgaaaaag
    13081 aaatggaact gctcgctcca catctgaacg gacggcacca gcttgcccaa ctgcacttcg
    13141 gcggcggcac accgaccttt ttgagcgacg aacagatcga acgtgtcttc cgcatgatcc
    13201 gcaaacattt cgaactgata ccgtccggcg aatattccat cgaaatcgac ccgcgcaaag
    13261 tcagccgcga caccgtcctc atgctcggca gactcggctt caaccgcatg agcgtcggca
    13321 ttcaggattt cgaccccaaa gtgcaggcgg cagtcaaccg catccaaagc tacgaagaaa
    13381 ccaaagaagt catcgatgcc gcccgtgagg cagggttcaa atccgtcagc gtcgatttga
    13441 tttacggctt gccgcatcag acttccgaaa gcatcaaaac caccatagat accgttttgt
    13501 cgctcgatcc cgaccgcctc gccctttacc actacgccca cctgccgcac gtgttcaaac
    13561 cgcaacgccg catcgatacc gccgccgtcc ccgacagcga agagaagctc gatatgctgc
    13621 aatactgcgt ccaaaccctg acagagcgcg gctacgtctt catcggcatg gaccatttcg
    13681 ccaaacctga cgacgaactc tccatcgccc tcaaagaagg cttcctccaa cgcaacttcc
    13741 aaggctattc gacctacgcg gattgcgatt tggtcgccat cggcgtgtcg tccatcggca
    13801 aaatcggcag cacctattcc caaaacgaac gcgacatcga tgcctactat gccgccatcg
    13861 acgaaggcag actgcccatc atgcgcggct accagctcaa tcaggacgac atcctgcgcc
    13921 gcaacatcat tcaggattta atgtgccgct tcgcgctcga ctacaggatt tacgaaagta
    13981 tgttcggcat cccgttcgac cgctacttca aagacgaact ggcggatttg gaaaaactcg
    14041 ccggtttggg attggtgcgc ctgaacagcc acgggctgac cgttacaccg aaaggacgct
    14101 tcctcatccg caacatcgcc atggtcttcg actaccacct gcgccacaaa gaaaccaaag
    14161 cgaaatattc gcaaacggtg tgattgcggc taacgtacaa aagccgtctg aaaggctttt
    14221 tcagacggca ttttgctgcc gaggataagt gttttcaaga acaggcggcg gcatatcata
    14281 acctttgtgt ccgaccgttc cgaaaccaag ataccaaaca gcatatcccg acagaacaga
    14341 ttgtgcataa ggcacaagcc cgcacattcc atcaacaaaa tgccgtctga acacgggttc
    14401 agacggcatc agtattttac aatcagaaca ctgcctgcaa aatcaaatag ctggccatcg
    14461 acaatacagc ggcggcaggc agggtaatga cccacgccaa accgatgggt ttcatcagtt
    14521 tccagttggc attgcggttg accagaccga taccgagtac cgcgccgacc aagatatgcg
    14581 tactggacac gggcagcccc atcagcgacg cgcccatcac gacggatgcg gcggacagtt
    14641 cggcggtaaa acccgaagca ggatgcattt ccgccaaact cgtaccgacg gttttaatca
    14701 cctctttacc gacaaaccac aaaccgacaa tcagcgcgat gccgaaagtc agcatcgcaa
    14761 tcggagggac ggcgctttgc gcggcaacgc tgttcgtccg caaaacatcc ataatcgcgg
    14821 caaacgggcc gatggcgttg gcgatatcgt tcgcaccgtg gctgaatgcg aagccgcagg
    14881 cggtaaagac ctgcatccat gaaaacatct gaaaggtcga tttgcccaag tctttacgct
    14941 tgaggctttt ggcaaacaca aacgtcccca tccacaccgc cgcgcctatc ataaagatgg
    15001 tcaggaagct gttgacgttg ctcatcccca aatgcaggtt tttcaagccc ttgaaaatca
    15061 gcatagcgga aatcatcatc gtgccgaacg aagcgataaa gggaatccaa gaatgcagcg
    15121 ccttgtagga atcgacattg tttttacggt tgtcgaacgc ataaagaccg cggtaatact
    15181 ccgattgcag ctcttgagga tcgaattcgg gttcgtcgta aatttgcgcg tcgtgcgcca
    15241 ttttggtggc gtactcgact ttttcggctt cggacaaacc ctcgaaaaac aggcggtgcc
    15301 gttctttata ggcctttttt tcctgcttga tgcccttgag cgttccttcc gcccaagcgt
    15361 tgtaatctaa gacgtttttc ttgacgcgcg aaaacaggaa ataggacacc gcgccgccca
    15421 acacgggcga caatacccaa gacatcccga tttcgcccag cttgccccac cgtatcaacg
    15481 ccaccccgtc cgcattatgc gtaaacgcca tacacaacgc actgccgacg atgccgccga
    15541 taatcgcgtg cgtcgtcgaa accggcaggc ctttgcggga cgcgaacagc agccacaacg
    15601 ccgccgccaa aagcgcggac atcataataa acacaaactg tatgggttcg aaatcaacac
    15661 ccttcaaatc gacgatgcct ttgcgtatgg tatcggttac ctcgccgccc gcgatgaccg
    15721 cgccgctgac ctcaaatacc gccgcaatca gcaaagcctg cgggatggtc agcgtgcctg
    15781 caccgacgct ggtgccgaaa gaattggcaa catcgttgcc gccgatgttg aacgccataa
    15841 acacaccgaa catcgtggca ataatgaata aggcggcgta attgtaatgg gtataaccca
    15901 agccccaata aataaaatag ccgactgtac ctaccagcat ggcggcaaag acggcgttga
    15961 tggttttaac atttgcgctc atttggattt gagccatggc aatcttcctt taatgtggtg
    16021 gaaatcatga gtgttgccga cactgtattg ctgcctccgg tcgaactgcc gtttcgggat
    16081 atggcagacg catccttcct tctgaaccgc atccgaatac ccccaaacct gaccgaatgc
    16141 aaaactgctg ccggaaacgg tttgtatgac acttctacta ctggatgttg cgggcgcatt
    16201 ataatatttt tccacccgtc ttaaaacatt tatttacact ttatttacac tgcggcggca
    16261 aatcggtata cgagcgtcaa tacacgttaa aatggcgttt tgcaccagtt tgggagtgat
    16321 gatggaaaca cagctttaca tcggcattat gtcgggaacc agtatggacg gggcggatgc
    16381 cgtgctggta cggatggacg gcggcaaatg gctgggcgcg gaagggcacg cctttacccc
    16441 ctaccctgac cggttgcgcc gcaaattgct ggatttgcag gacacaggca cagacgaact
    16501 gcaccgcagc aggatgttgt cgcaagaact cagccgcctg tacgcgcaaa ccgccgccga
    16561 actgctgtgc agtcaaaacc tcgctccgtg cgacattacc gccctcggct gccacgggca
    16621 aaccgtccga cacgcgccgg aacacggtta cagcatacag cttgccgatt tgccgctgct
    16681 ggcggaactg acgcggattt ttaccgtcgg cgacttccgc agccgcgacc ttgctgccgg
    16741 cggacaaggt gcgccgctcg tccccgcctt tcacgaagcc ctgttccgcg atgacaggga
    16801 aacacgcgtg gtactgaaca tcggcgggat tgccaacatc agcgtactcc cccccggcgc
    16861 acccgccttc ggcttcgaca cagggccggg caatatgctg atggacgcgt ggacgcaggc
    16921 acactggcag ctgccttacg acaaaaacgg tgcaaaggcg gcacaaggca acatattgcc
    16981 gcaactgctc ggcaggctgc tcgcccaccc gtatttctca caaccccacc caaaaagcac
    17041 ggggcgcgaa ctgtttgccc taaattggct cgaaacctac cttgacggcg gcgaaaaccg
    17101 atacgacgta ttgcggacgc tttcccgatt caccgcgcaa accgtttgcg acgccgtctc
    17161 acacgcagcg gcagatgccc gtcaaatgta catttgcggc ggcggcatcc gcaatcctgt
    17221 tttaatggcg gatttggcag aatgtttcgg cacacgcgtt tccctgcaca gcaccgccga
    17281 actgaacctc gatcctcaat gggtggaggc ggccgcattt gcgtggttgg cggcgtgttg
    17341 gattaaccgc attcccggta gtccgcacaa agcgaccggc gcatccaaac cgtgtattct
    17401 gggcgcggga tattattatt gagcgcaaat accgtctgaa agccatttcc gccgttcaga
    17461 cggcatttcc acccctttcg tttacaaacc cttaaaatcc atttacactc aaacccgttc
    17521 aaaacaaaac aaaccctgcc atgaaaaccc tgctcctcct catccccctc gtactcaccg
    17581 cctgcggcac actgaccggc atacccgccc tgaaaggacg caaagccgcc ctttacgtct
    17641 ccgttatggg cgaccaaggt tcgggcaaca taagcggcgg acgctactcc atcgacgcac
    17701 tgatacgcgg cggctaccac aacaaccccg acagcgccac ccgatacagc taccccgcct
    17761 atgacactac cgccaccacc aaatccgacg cgctctccgg cgtaaccact tccacatcgg
    17821 tcttaaacgc ccccgccgcc gccctgacga aaaacaacgg acgcaaaggc gaacgctccg
    17881 ccggactgtc cgtcaacggc acgggcgact accgcaacga aaccctgctc gccaaccccc
    17941 gcgacgtttc cttcctgacc aacctcatcc aaaccgtctt ctacctgcgc ggcatcgaag
    18001 tcgtaccgcc cgaatacgcc gacaccgacg tattcgtaac cgtcgacgta ttcggcaccg
    18061 tccgcagccg taccgaactg cacctctaca acgccgaaac ccttaaagcc caaaccaagc
    18121 tcgaatattt cgccgtcgac cgcgacagcc ggaaactgct gattgcccct aaaaccgccg
    18181 cctacgaatc ccaataccaa gaacaatacg ccctctggat gggaccttac agcgtcggca
    18241 aaaccgtcaa agcctcagac cgcctgatgg tcgatttctc cgacatcacc gcctacggcg
    18301 acacaaccgc ccaaaaccgt cccgacttca aacaaaacaa cggtaaaaac cccgatgtcg
    18361 gcaacgaagt catccgccgc cgcaaaggag gataaaccgt gaaaccgctg cgcagactga
    18421 caaacctcct tgccgcctgc gccgtagcgg cggccgcact catacagccc gccctcgcgg
    18481 cggacttggc gcaagacccg ttcattaccg ataacaccca acggcagcac tacgaacccg
    18541 gcggcaaata ccacctcttc ggcgacccgc gcggcagcgt ttccgaccgc accggcaaaa
    18601 tcaacgtcat ccaagactat acccaccaga tgggcaacct gctcatccaa caggcggcaa
    18661 tccaaggcaa tcttggttac accgtccgct tttccggaca cggacacgaa gaacacgccc
    18721 ccttcgacaa ccacgccgcc gacagcgcaa gcgaagaaaa aggcaacgtt gacgacggct
    18781 ttaccgtgta ccggctcaac tgggaaggac acgaacatca tcccgccgat gcctacgacg
    18841 gcccgaaggg cggcaattac cccaaaccta cgggcgcacg agacgaatac acctatcacg
    18901 tcaacggcac agcccgcagt atcaaactca atccgaccga cacccgcagc atccggcaac
    18961 gcatatccga caactacaac aacctcggca gcaatttctc cgaccgcgcc gatgaagcca
    19021 acagaaaaat gttcgagcac aatgccaagc tcgaccgccg gggcaacagc atggagtttg
    19081 tcaacggcgt cgccgccggc gcgctcaacc cctttatcag cgcgggcgaa gccttgggca
    19141 taggcgacat actgtacgga acgggttatg ccatagacaa agccgcgatg cgcaacatcg
    19201 cccccttgcc cgccgagggt aaattcgccg tcatcggcgg cttgggcagc gcggcgggct
    19261 ttgaaaagaa tacgcgcgaa gccgttgacc ggtggataca ggaaaacccc aatgccgccg
    19321 aaaccgtcga agccgtcttc aacgttgccg cagcagccaa agtcgcgaag ttggcaaagg
    19381 cggcaaagcc ggggaaagct gcggttagcg gggatttctc aaaatcctac acctgctctt
    19441 tccacggcag caccttggtc agaacggcag acggctacaa agccatcgcc catattcaag
    19501 ccggagaccg cgtcctttcc aaggacgagg caagcggaga aacgggatac aaacccgtta
    19561 ccgcccgata cggcaatccg taccaagaaa ccgtttacat taaagtttca gacggcatcg
    19621 gcaacagcca aaccctgatt tccaaccgca tccatccgtt ttattcggac ggcaaatgga
    19681 ttaaagcgga agatttgaaa gcgggaagca ggctgtttgc tgaaaacggt gcagagcaga
    19741 ccgttcaaag cgttaccgtc aaaccggaac cgctcaaagc ctacaatctg accgtcgccg
    19801 actggcatac ttatttcgtc aagggtagtc aggcggaaac ggaaggagtt tgggttcata
    19861 atgattgtcc gcctaaacca aaaccaacca atcatgccca acaaagaaaa gaagaagcta
    19921 aaaacgattc tcatcgaagt gtgggagatt ccaatcgtgt cgttcgcgaa ggaaagcaat
    19981 atttagattc cgacacagga aaccatgttt atgtaaaagg agataaagtg gttattctaa
    20041 ctcctgatgg aagacaggta actcaattta agaactcgaa agccaatacg tcaaaaaggg
    20101 taaaaaatgg gaaatggaca ccaaaataat caatcttcct tagaaaaaag aattttttat
    20161 ctggagcatt cggggcagta tctgatgatt tgcgccctat cggattactc ccaaaataaa
    20221 catactgtcg ttatggcgaa tttcctctat ccaaatgaaa aaatggattg gagaaattta
    20281 gatgatttgt tcaatgagtt ggttttagag gaattacagt cttcatttat ggattggtat
    20341 ccaactattg aaaaggcaat tagccatcat ttagaagact tttcgtaaca agcataaatc
    20401 aaaaagtcgt ctgaaactgc ttttaatttt cagacgacct ttccccatat cggcggatta
    20461 tgaatttaca gctatcggga aattgagtta tctctctgat agagaaaatt ccatacgcga
    20521 agccgttgac cggtggatac aggaaaatcc caatgccgcc gaaaccgtcg aagccgtctt
    20581 caacgttgcc gccgccaaag tcgcgaagct ggcaaaggcg gcgaaaccgg ggaaagctgc
    20641 ggttagcggg gatttttcga ttagctataa aaacttttct actgtcaaac caaaagtaat
    20701 tgccaaagga acgattaatg gaaagacttt tagagatgtt aatcaaagtg caaaaattgg
    20761 atcgcctgat tcacctacat taatagccca acgagttaat gcaaaaatcc aagccgacgg
    20821 taaacctcgt ccgaatgcaa ccgttgctaa ttcacatgct gaaattggtg ttatccagca
    20881 ggcttataat gcaggtgaaa cgaaaggggc atctatgaca atgacagttt caggaaaaga
    20941 tgtttgcggt tattgtaaag gagatattgc agctgctgct caagcttctg gactgaaatc
    21001 actgactgtt aatgcgacag ataatgtaac aggaaaaaat aaaacttact attggacgcc
    21061 tggtatgaaa tcaattaagg agagaaaatg aaaacatcaa caattgtttt tggtggattt
    21121 tttattacag ataatggaga acgaatccaa atccctattt tagaaaatcc taacattaaa
    21181 gaaataaata actttttttc tgtatcaaat tttgaaaaaa aagccggagt ccttgttttc
    21241 agaattattc ctgagcctga atttggcaat actgaattaa ctatctattt tgaaaaagga
    21301 tattatttac caataattca aacaatttta gaagatggag atattgaagt aaaaaatctt
    21361 aaaacagaaa attatagtgg aaatacaatg gaaattttgg gagacgtcta ccctattgag
    21421 catatctcta aaaatatttc tataattcaa gatataattt ctgaatttat tatgaaaaat
    21481 aaaccaatca caataatgat ttaattgaaa agttcaacaa tcaaaaggtc gcctgaaatc
    21541 gtgtttaatt ttcagacgac ctatttcctt catttgaaac aggatattga aaactaagtt
    21601 cttcaaaaat cctacacctg ccctttccac ggcagcacct tggtcaaaac ggcagacggc
    21661 tacaaagcca ttgcccgtat ccgggtcggc gaccacgtct tcgccaagga cgaggcaagc
    21721 ggggagacgg gatacaaacc cgttaccgcc caatacggca atccgtatcg ggaaaccgtt
    21781 tacattgaaa tttcagacgg catcggcaac a
//
LOCUS       DAWTQS010000031        21609 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.031, whole genome shotgun sequence.
ACCESSION   DAWTQS010000031 DAWTQS010000000
VERSION     DAWTQS010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21609
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.031"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            <1..1597
                     /locus_tag="WNA25_001155"
     CDS             <1..1597
                     /locus_tag="WNA25_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HFA9823914.1"
                     /translation="AWLWPGVFSHDLWNPAEPAVYTAVEALAGSPTPLVAHLFGQTDF
                     GIPPVYLWVAAAFKHLLSPWAADPYDAARFAGVFFAVIGLTSCGFAGFNFLGRHHGRS
                     VVLIHIGCIGLIPVAHFLNPAAAAFAAAGLVLHGYSLARRRVIAASFLLGTGWTLMSL
                     AAAYPAAFALMLPLPVLMFFRPWQSRRLMLTAVASLAFALPLMTVYPLLLAKTQPALF
                     AQWLNYHVFGTFGGVRHIQTAFSLFYYLKNLLWFALPALPLAVWTVCRTRLFSTDWGI
                     LGIVWMLAVLVLLAFNPQRFQDNLVWLLPPLALFGAAQLDSLRRGAAAFVNWFGIMAF
                     GLFAVFLWTGFFAMNYGWPAKLAERAAYFSPYYVPDIDPIPMAVAVLFTPLWLWAITR
                     KNIRGRQAVTNWAAGVTLTWALLMTLFLPWLDAAKSHAPVVRSMEASFSPELKRELSD
                     GIECIGIGGGDLHTRIVWTQYGTLPHRVGDVRCRYRIVRLPQNADAPQGWQTVWQGAR
                     PRNKDSKFALIRETGENILKTTD"
     gene            1655..1759
                     /locus_tag="WNA25_001156"
                     /pseudo
     CDS             1655..1759
                     /locus_tag="WNA25_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            1804..2409
                     /locus_tag="WNA25_001157"
     CDS             1804..2409
                     /locus_tag="WNA25_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HFA9823915.1"
                     /translation="MMTLYSGITCPFSHRCRFVLYEKGMDFEIKDIDIYNKPEDLAVM
                     NPYNQVPVLVERDLVLHESNIINEYIDERFPHPQLMPGDPVMRGRGRLVLYRMEKELF
                     NHVQVLENPAAANKEQAKAREAIGNGLTMLSPSFSKSKYILGEDFSMIDVALAPLLWR
                     LDHYDVKLGKSAAPLLKYAERIFQREAFIEALTPAEKAMRK"
     gene            2481..2873
                     /locus_tag="WNA25_001158"
     CDS             2481..2873
                     /locus_tag="WNA25_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFA9823916.1"
                     /translation="MPTSTKPYILRALCEWCSDNSLTPHILVWVNEHTRVPMQYVRDN
                     EIMLNIGATATQNLRIDNDWISFSARFGGQAHDIWIPVGHVLSLFARETGEGMGFELE
                     EYRPDTPSENTSAETAPRPAKKGLKLVK"
     gene            2878..3024
                     /locus_tag="WNA25_001159"
     CDS             2878..3024
                     /locus_tag="WNA25_001159"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823917.1"
                     /translation="MPSERNRVSDGILSDGAQTESVYRQNLFRRIETVLPLPIRYWDI
                     ENAV"
     gene            complement(3072..3518)
                     /locus_tag="WNA25_001160"
     CDS             complement(3072..3518)
                     /locus_tag="WNA25_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFA9823918.1"
                     /translation="MSLKTRLTEDMKTAMRAKDQVSLGTIRLINAAVKQFEVDERTEA
                     DDAKITAILTKMVKQRKDGAKIYTEAGRQDLADKENAEIDVLHRYLPQMLSAGEIRTA
                     VEAAVAETGAAGMADMGKVMVVLKTRLAGKADMGEVNKILKTVLTA"
     gene            complement(3650..3862)
                     /locus_tag="WNA25_001161"
     CDS             complement(3650..3862)
                     /locus_tag="WNA25_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFA9823919.1"
                     /translation="MPAIRVKENEPFEVAMRRFKRAVEKTGLLTELRAREAYEKPTTE
                     RKRKKAAAVKRLQKRLRSQQLPPKMY"
     gene            complement(4151..6001)
                     /locus_tag="WNA25_001162"
     CDS             complement(4151..6001)
                     /locus_tag="WNA25_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFA9823920.1"
                     /translation="MYLPSMKHSLPLLAALVLAACSSTNTLPAGKTPADNIETADLSA
                     SVPTRPAEPEGKTLADYGGYPSALDAVKQNNDAAAAAYLENAGDSAMAENVRNEWLKS
                     LGARRQWTLFAQEYAKLKPEGRAQEVECYADSSRNDYTRAAELVKNTGKLPSGCTKLL
                     EQAAASGLLDGNDAWRRVRGLLAGRQTTDARNLAAALGSPFDGGTQGSREYALLNVIG
                     KEARKSPNAAALLSEMESGLSPEQRSFAWGVLGHYQSQSLNVPAALDYYGKVADRRQL
                     TDDQIEWYARAALRARRWDELASVISHMPEKLQKSPTWLYWLARSRAATGNTQEAEKL
                     YKQAAATGRNFYAVLAGEELGRKIDTRNNVPDADTNSVLRMAEDGAIKRALVLFQHSR
                     TAGDAKMRRQAQAEWRFATRGFDEDKLLAAAQTAFDHGFYDMAVNSAERTDRKLNYTL
                     RYISPFKDTVIRHAQNVNVDPAWVYGLIRQESRFVIGAQSRVGAQGLMQVMPATAREI
                     AGKIGMDAAQLYTADGNIRMGTWYMADTKRRLQNNEILATAGYNAGPGRARRWQADTP
                     LEGAVYAETIPFSETRDYVKKVMTNAAYYASLFGAPHIPLKQRMGTVPAR"
     gene            6378..7115
                     /locus_tag="WNA25_001163"
     CDS             6378..7115
                     /locus_tag="WNA25_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFA9823921.1"
                     /translation="MIILDKVSKHYQTRDKTRFAAVEPTSLEIQDGEIFGLMGYSGAG
                     KSTLLRLINLLERPDTGKVNVCGQELTALDAAALRQARQNIGMVFQQFNLLSNRTVAG
                     NVAFPLEIAGWPSEKIKARVAECLEIVGLTERAGHYPAQLSGGQKQRVGIARALAPKP
                     QVILADEPTSALDPATTRSVLECLEDINKRFNVTIVIVTHEMSVIRRLCDRAALLDKG
                     KVVEIVEVRGNQIHAQSEIGRELIRED"
     gene            7117..7803
                     /locus_tag="WNA25_001164"
     CDS             7117..7803
                     /locus_tag="WNA25_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFA9823922.1"
                     /translation="MADLTFEQAVSTIVGMKDEIVRALGETFVMVGLSTTFAVIFGTL
                     LGVLLFVTSSRQLHYNKPVNFLLDNLVNLMRAFPFVILMIAMIPATRAIVGSTIGPVA
                     ASLVLSVSGLFYFARLVEQNLREVPKGVIEAAAAMGAPPIAIVLKVLLNEARAGMVSS
                     ITVLAIGLLSYSAAAGMIGGGGLGDLAIRYGYYRYQTEVIVFIVALLVLLVILIQSTG
                     NALARKLDKR"
     gene            7962..8828
                     /locus_tag="WNA25_001165"
     CDS             7962..8828
                     /locus_tag="WNA25_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823923.1"
                     /translation="MKTFFKTLSAAALALILAACGGQKDSAPAASAAAPSADNGAAKK
                     EIVFGTTVGDFGDMVKEQIQAELEKKGYTVKLVEFTDYVRPNLALAEGELDINVFQHK
                     PYLDDFKKEHNLDITEAFQVPTAPLGLYPGKLKSLEEVKDGSTVSAPNDPSNFARALV
                     MLNELGWIKLKDGINPLTASKADIAENLKNIKIVELEAAQLPRSRADVDFAVVNGNYA
                     ISSGMKLTEALFQEPSFAYVNWSAVKTADKDSQWLKDVTEAYNSDAFKAYAHKRFEGY
                     KYPAAWNEGAAK"
     gene            complement(8891..9460)
                     /locus_tag="WNA25_001166"
     CDS             complement(8891..9460)
                     /locus_tag="WNA25_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFA9823924.1"
                     /translation="MVRRLIIGISGASGFQYGVKALELLRAQDVETHLVVSKGAEMAR
                     ASETDYTKDEVYALADFVHPIGNIGACIASGTFKTDGMLVAPCSMRTLASVAHGFGDN
                     LLTRAADVVLKERRRLVLMVRETPLNLAHLDNMKRVTEMGGVVFPPVPAMYRKPQTAD
                     DIVAHSIAHTLSLFGIDTPDLAEWQGMAD"
     gene            9618..10478
                     /locus_tag="WNA25_001167"
     CDS             9618..10478
                     /locus_tag="WNA25_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFA9823925.1"
                     /translation="MAKVKGGLGRGLDSLLANGADNSSGDRLTAVAVKDIRPGRYQAR
                     VQIDDEALQELADSIKAQGVIQPVIVREHGLSRYELIAGERRWRAAQIAGLSEIPAVI
                     KTISDETALAMGLIENLQRENLNPIEEAQGLKRLADEFGLTHETIAQAVGKSRSAISN
                     SLRLLSLPESVQEMLYQRRLEMGHARALLTLPVVEQLELAQKAVKNGWSVREVERRSQ
                     AALQNKRPEPKKTAAADIGRLNDLLTEKLGVNAEIKTANHKKGKIILHFDTPETFDHI
                     LKQLGIDYRP"
     gene            11153..11506
                     /locus_tag="WNA25_001168"
     CDS             11153..11506
                     /locus_tag="WNA25_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFA9823926.1"
                     /translation="MKQIIILQSAVLSICAAVAFAVWGFAGFLSAVGGGLSYLLPTFV
                     AVLLLKLFRGNPFLQSRMFVFGEILKVVLSLLSMLAVFAIWHQSLVFVPFLMGLLGVS
                     HLVFLVLLRVKDYGR"
     gene            11496..12362
                     /gene="atpB"
                     /locus_tag="WNA25_001169"
     CDS             11496..12362
                     /gene="atpB"
                     /locus_tag="WNA25_001169"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFA9823927.1"
                     /translation="MAGETITAADYIKHHLQSLTSLSDVTQGQGLKNIADFSFINLDA
                     VFFAVLLGVIGSFLLWRGAKKATAGVPGRFQAAVEILFEFVDDMCKSIIHSEKSRKAV
                     APLGLTLFVWIFLMNAMDMLPVDLLPMVWQGITGNHHALLRIVPTADLNTALALAVGV
                     LLICIYYNIKIKGLGGWFHELFSAPFGAKLAPANFLLNLVEFLSKTVSHGMRLFGNMY
                     AGELVFLLIALLGGAWAASGSVEVMDPILFVFHIIAGLAWAIFHILVITLQAFIFMAL
                     AFVYIGQAHDAH"
     gene            12419..12655
                     /gene="atpE"
                     /locus_tag="WNA25_001170"
     CDS             12419..12655
                     /gene="atpE"
                     /locus_tag="WNA25_001170"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFA9823928.1"
                     /translation="MGLIAIACGLIVALGALGASIGIAMVGSKYLESSARQPELIGPL
                     QTKLFLIAGLIDAAFLIGVAIALLFAFVNPFAGA"
     gene            12725..13195
                     /locus_tag="WNA25_001171"
     CDS             12725..13195
                     /locus_tag="WNA25_001171"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFA9823929.1"
                     /translation="MNINATLFAQIIVFFGLVWFTMKFVWPPIAKALDERAAKIAEGL
                     AAAERGKSDFEQAEKKVAELLAEGRNQVSEMVANAEKRAAKIVEEAKEQASSEAARIA
                     AQAKADVEQELFRARESLRDQVAVLAVKGAESILRSEVDASKHAKLLDTLKQEL"
     gene            13200..13733
                     /locus_tag="WNA25_001172"
     CDS             13200..13733
                     /locus_tag="WNA25_001172"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFA9823930.1"
                     /translation="MAEFATIARPYAKALFGLAQEKNQIESWLGGLEKLAAVVQEGKV
                     ALLIDRPETNASEKADILIDLVGLKDKELKNFVIVLAGQKRLSILPEVYAQYQDLTLS
                     FNHIKSAVIYSAYPLTDKQVGELAQMLNKRFDSELKISVEIEPELIGGIKVEVGDQVL
                     DLSVQGKLSALYTTMTN"
     gene            13744..15291
                     /locus_tag="WNA25_001173"
     CDS             13744..15291
                     /locus_tag="WNA25_001173"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFA9823931.1"
                     /translation="MQLNPAEISDLIKAKIENLSVNAEVSTRGTVISVTDGIVRIHGL
                     SDAMQGEMLEFPGNTLGLAMNLERDSVGAVVLGEYEHIKEGDTVTCTGRILEVPVGRE
                     LVGRVVDALGRPIDGKGPINTTLTAPVEKIAPGVIARKSVDQPMQTGLKAIDSMIPVG
                     RGQRELIIGDRQTGKTAVALDAIVNQKGTGVICIYVAIGQKASSIANVVRKLEEHGAM
                     EHTIVVAATASEAAALQYIAPYSGCTMGEFFRDRGEDALIVYDDLSKQAVAYRQISLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVNEHEVEKLTNGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETDLFNAGIRPAINAGISVSRVGGAAQTKVIKKLGGG
                     IRLALAQYRELAAFSQFASDLDEATRKQLEHGEVVTELMKQKQFSTLNTAEMALTLWA
                     INNGSYSDVPVAKALAFESEFLSFVRTQHPEVLEAVNASGAMSDESEKTLEAAMKSFK
                     SSYAYQA"
     gene            15316..16191
                     /gene="atpG"
                     /locus_tag="WNA25_001174"
     CDS             15316..16191
                     /gene="atpG"
                     /locus_tag="WNA25_001174"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFA9823932.1"
                     /translation="MAVGKEILTKIRSVQNTQKITKAMQMVSTSKMRKTQERMRLARP
                     YAEKVRMVMSHLAQTNTDHGIPLLESHREIRRVGFILITSDKGLCGGLNANVLKKFLA
                     QVQEYRNQGIEEEIVCLGSKGLMACQSIGLNVVASAVNLGDTPKMEMLLGPLTELFQR
                     YEKHEIDKIHLVYSGFVNTMRQEPRMEVLLPIGENVIGDSAPKPPFSWEYRYEPTALA
                     VLEYLVRRYLESVVYQALSDNMASEQAARMVAMKAATDNAGNAIKELRLVYNKSRQAA
                     ITTELSEIVAGAAAV"
     gene            16229..17626
                     /gene="atpD"
                     /locus_tag="WNA25_001175"
     CDS             16229..17626
                     /gene="atpD"
                     /locus_tag="WNA25_001175"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFA9823933.1"
                     /translation="MSQGKIVQIIGAVVDVEFPRDMIPRVYDALKLDENGLTLEVQQL
                     LGDGVVRTIAMGSSDGLKRGMTVSNTGSPITVPVGKGTLGRIVDVLGTPVDEAGPIDT
                     DKSRAIHQAAPKFDELSSTTELLETGIKVIDLLCPFAKGGKVGLFGGAGVGKTVNMME
                     LINNIAKAHSGLSVFSGVGERTREGNDFYHEMKDSNVLDKVAMVYGQMNEPPGNRLRV
                     ALTGLTMAEYFRDEKDENGKGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTL
                     AEEMGRLQERITSTQTGSITSIQAVYVPADDLTDPSPATTFAHLDATVVLSRDIASLG
                     IYPAVDPLDSTSRQLDPMVLGQEHYDVARGVQSTLQKYKELRDIIAILGMDELSDEDK
                     LAVMRARKIQRFLSQPFHVAEVFTGSPGKYVALRDTIAGFKAILNGEYDHLPEQAFYM
                     VGSIEEAVEKAKTLN"
     gene            17637..18059
                     /locus_tag="WNA25_001176"
     CDS             17637..18059
                     /locus_tag="WNA25_001176"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFA9823934.1"
                     /translation="MSIMQVEVVSGEQKIYSGEATFIVVPTVQGELGIYPRHEPIMSL
                     VRPGALRLTVPGEDKEVLVAVSGGILEVQPDKVTVLADVAVRSAEMDRARAEEAKKAA
                     EAGISQAKDDKALAEAHKALAAAIAQLKTLDYIRSHKK"
     gene            18231..19127
                     /gene="glyQ"
                     /locus_tag="WNA25_001177"
     CDS             18231..19127
                     /gene="glyQ"
                     /locus_tag="WNA25_001177"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFA9823935.1"
                     /translation="MLTFQQIIFKLQTFWADKGCTVIQPFDMEVGAGTSHPATCLRAL
                     GPEPWFAAYVQPSRRPKDGRYGDNPNRLQHYYQFQVALKPAPANIQDLYLDSLRELGI
                     DPKVHDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCTPVLGEITY
                     GIERLAMYLQGVENVYDLVWAKTPDGNTVSYGDVYHQNEVEQSTYNFEYSDADWLLRQ
                     FNDYEAQAKRLFAEENADLALPAYELVLKAGHTFNLLDARGAISVTERATYIGRIRAL
                     SRAVAQKYIESREKLGFPLIKK"
     gene            19244..21307
                     /locus_tag="WNA25_001178"
     CDS             19244..21307
                     /locus_tag="WNA25_001178"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFA9823936.1"
                     /translation="MTTQTLLIELLTEELPPKALNNLGNHFAASVAEGLEKAQLVDGA
                     AEFTAYASPRRLAVQVKNVKAVQADQKIVKKGPAVANAVKDGAPTKALEGFARGAGAK
                     IEDLTIVHDGRQDVYAYEYVQTGKPLGGLLENIINQAVKKLPIPKVMRWGSSTFTFVR
                     PVHGLIVLHGGDVVNVSVLGLQSGNQTLGHRFLSDGEIIIENADSYAAQMRGQGKVVA
                     SFAGRKAAIQTALEGQARRLNATVAADEALLDEVTALVEWPVVLEAGFEEHFLAVPQE
                     CLILTMQQNQKYFPLLDQNGKLMNRFLLVSNLQTEDPSHIIRGNERVLRARLSDAEFF
                     YKQDQKATLESRLPKLANVVYHNKIGSQAERIERLQSIAAHIAKALGADAAAAGRAAR
                     LAKADLVTEMVGEFPELQGTMGKYYARLDGETEEIAEAIEQHYQPRFAGDKLPESKIA
                     AAVALADKLETLVGIWGIGLIPTGDKDPYALRRAALGILRMLMQYGLDVNELIQTAFD
                     SFPQGLLNEKTPSETADFMQARLAVLLQNDYPQDIVAAVLAKQPRRLDDLTAKLQAVA
                     VFKQLPEAAALAAANKRVQNLLKKADAELGAVNESLLQQDEEKALYAAAQGLQPKIAA
                     AVAEGNFQTALSELASVKPQVDAFFDGVMVMAEDAAVKQNRLNLLNRLAGQMNAVADI
                     ALLGE"
     gene            21371..>21609
                     /locus_tag="WNA25_001179"
     CDS             21371..>21609
                     /locus_tag="WNA25_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFA9823937.1"
                     /translation="MQPLVSVLICAYNVEKYFAQSLAAVVNQTWRNLDILIVDDGSTD
                     GTLAIAKDFQKRDSRIKILAQAQNSGLIPSLNIGL"
BASE COUNT         4991 a         5802 c         5733 g         5083 t
ORIGIN      
        1 tgcctggctg tggcccggcg tgttttccca cgatttgtgg aatcctgccg aacctgccgt
       61 ctataccgcc gtcgaagcac tggcaggcag ccccaccccc ttggttgccc atctgttcgg
      121 tcaaaccgat ttcggcatac cgcccgtgta tctttgggtt gccgccgcat tcaaacattt
      181 gctgtcgccg tgggcagccg acccgtatga tgccgcacgc tttgcaggcg tattttttgc
      241 cgttatcgga ctgacttctt gcggctttgc cggtttcaac tttttgggca gacaccacgg
      301 gcgcagcgtt gttttaatcc atatcggctg tatcgggctg attccggttg cccatttcct
      361 caatcccgcc gccgccgcct ttgccgccgc cggactggtg ctgcacggct actcgctggc
      421 acgccggcgc gtgattgccg cctctttcct gctcggtacg ggttggacgt tgatgtcgct
      481 ggcggcagct tatccggcgg cgtttgcgct gatgctgccc ctgcccgtgc tgatgttttt
      541 ccgtccgtgg caaagcaggc gtttgatgtt gacggcagtc gcctcgcttg cctttgccct
      601 gccgcttatg accgtttacc cgctgctctt ggcaaaaacg cagcccgcgc tgtttgcgca
      661 atggctcaac tatcacgttt tcggtacgtt cggcggcgtg cggcacattc agacggcatt
      721 cagtttgttt tactatctga aaaatctgct ttggttcgca ctgcccgcgc tgccgctggc
      781 ggtttggacg gtttgccgca cacgcctgtt ttcgaccgac tgggggattt tgggcattgt
      841 ctggatgctt gccgttttgg tgctgctcgc ctttaatccg cagcgttttc aagacaacct
      901 cgtctggctg ctgccgccgc ttgccctgtt cggcgcggcg caactggaca gcctgaggcg
      961 cggcgcggcg gcttttgtca actggttcgg cattatggcg ttcgggctgt ttgccgtgtt
     1021 cctgtggacg ggctttttcg ccatgaatta cggctggccc gccaagcttg ccgaacgcgc
     1081 cgcctacttc agcccgtatt acgttcccga catcgatccc attccgatgg cggttgccgt
     1141 actgttcaca cccttgtggc tgtgggcgat tacccggaaa aacatacgcg gcaggcaggc
     1201 ggttaccaac tgggcggcag gcgttaccct gacctgggct ttgctgatga cgctgttcct
     1261 gccgtggctg gacgcggcga aaagccacgc gcccgtcgtc cggagtatgg aggcatcgtt
     1321 ttccccggaa ttaaaacggg agctttcaga cggcatcgag tgtatcggca taggcggcgg
     1381 cgacctgcac acgcggattg tttggacgca gtacggcaca ttgccgcacc gcgtcggcga
     1441 tgtccgttgc cgctaccgta tcgtccgcct gccccaaaac gcggatgcgc cgcaaggctg
     1501 gcagacggtc tggcagggtg cgcgcccgcg caacaaagac agtaagtttg cactgatacg
     1561 ggaaaccggg gaaaatatat taaaaacaac agattgagcc gaatttctgg attaagtgcc
     1621 ggaaatcgcg tataattgcg cgattaaacc tttatatagt ggattaaatt taaatcagga
     1681 caaggcgacg aagccgcaga cagtacaaat agtacggcaa ggcgaggcaa cgccgtaccg
     1741 gtttaaattt aatccactat aaatcagccg ttttgcaggc atcacacagg agcgacaatt
     1801 attatgatga ccctctattc cggcattacc tgccccttca gccaccgctg ccgcttcgtt
     1861 ttgtacgaaa aaggtatgga ttttgaaatc aaagacatcg atatttacaa caagcccgaa
     1921 gacctcgccg tcatgaaccc gtacaaccaa gtccccgtgc tggtcgagcg cgatttggtg
     1981 ctgcacgagt ccaacatcat caacgaatac atcgacgaac gcttccccca tccgcagctt
     2041 atgcccggcg atcccgttat gcgcggccgg gggcggctgg tgctgtaccg tatggaaaaa
     2101 gaattgttca accacgtcca agttttggaa aaccccgccg ccgccaacaa ggaacaggca
     2161 aaagcgcgcg aagccatcgg caacggcctg acgatgcttt ccccttcgtt cagcaaaagc
     2221 aaatacatcc tcggcgaaga tttttctatg attgatgttg ccctggcccc gctgttgtgg
     2281 cggctcgacc attacgatgt caaactgggc aaaagtgccg cgccgctgct caaatacgcc
     2341 gagcgtatct tccagcgcga agcctttatc gaagcactga cacccgccga aaaagccatg
     2401 cgcaaataag tccgaaatgc ttgcaaaacc cgccgttttg caggcatttt cacattcagg
     2461 cacaccacac ggaacccatc atgcccacct caaccaaacc ctacatcctc cgcgcacttt
     2521 gcgaatggtg cagcgacaac agcctcacgc cgcacatcct tgtctgggta aacgagcata
     2581 cgcgcgtccc catgcagtac gtccgcgaca acgaaattat gctcaacatc ggcgcaaccg
     2641 ccacgcaaaa ccttcgaatc gacaacgatt ggatcagctt ttccgcccgt ttcggcggac
     2701 aagcgcacga catatggata cctgtcggac acgtcctcag ccttttcgca cgggagaccg
     2761 gagaaggtat ggggtttgaa ttggaggagt accgccccga cacgccgtct gaaaacactt
     2821 ctgccgaaac cgcgccccgg cccgccaaaa aaggtttgaa attggtcaaa taaatctatg
     2881 ccgtctgaac ggaatcgtgt ttcagacggc attttgtccg atggggcgca aacggaatcc
     2941 gtttatcggc aaaacctgtt tcggcgtatc gaaaccgtgt tgcccctgcc gattcgatat
     3001 tgggacattg aaaatgccgt ctgaacctgc gatacgggct tcagacggcg ttttgtccga
     3061 tattcgggca atcaggcggt cagtacggtt ttcaagattt tgttgacttc gcccatatcg
     3121 gctttgccgg cgaggcgggt tttcaatacg accatcactt tgcccatatc cgccatacct
     3181 gccgcgccgg tttcggcaac ggctgcttcg acggcggtgc ggatttcgcc ggcggagagc
     3241 atttgcggca ggtagcggtg cagcacgtcg atttcggcgt tttctttgtc tgccaaatcc
     3301 tgacggccgg cttcagtgta gattttcgcg ccgtctttgc gctgtttgac cattttggtc
     3361 aggatggcgg tgattttggc atcgtcggct tcggtgcgtt cgtctacttc aaactgtttg
     3421 acggcggcat tgatgaggcg gatggtgccg agggaaactt gatctttggc gcgcatcgcg
     3481 gttttcatat cttcggtaag gcgggttttc aggctcatga tgtcctcgct gggatgtcgg
     3541 atggaaacgg cgggtttcga tgccgtctga aaagcaaaac acaccgcaga gcaggtcttg
     3601 cggtgtgtct tcatatcaca gggctgacct gtaatctgta cttgaacgtt tagtacattt
     3661 tgggcggcag ctgttggctg cgcaggcgtt tttgcaggcg ttttacggct gccgcttttt
     3721 tgcgtttgcg ttcggtagtc ggtttttcgt aggcttcgcg ggcgcgcagc tcggtcagca
     3781 ggccggtttt ttctacggcg cgtttgaaac ggcgcatagc gacttcaaat ggttcattct
     3841 cttttacgcg gattgcaggc attttatttc ctttaataaa ttcggttgtt ccatctgccc
     3901 atcatatcgg tggaaagggt agggcagacg gtgttgtagg ttttccgtat cgctgtgcct
     3961 ttcggcggcg ggatacgcgc cgtgacggaa acatcacctc ctaacgggcc ggcttgtgcc
     4021 gtgtcaagct gcttgaaaaa gtaggaaaaa caattttcgg attgtcttat atttatggtt
     4081 cgccgtcaat gccgttcggg ataaaaatgc cgtctgaaag accgggcggg tttcagacgg
     4141 catcggcacg tcagcgtgcg ggaacggtgc ccatacgctg tttgagcggg atgtgcggcg
     4201 cgccgaagag ggaggcgtag taggcggcat tggtcatcac ttttttgaca tagtcgcgcg
     4261 tttcggaaaa cgggatggtt tcggcatata ccgcgccttc gaggggtgtg tccgcctgcc
     4321 atcggcgcgc tctgccgggg ccggcgttat agcctgcggt ggcgaggatt tcgttgtttt
     4381 gcaggcggcg tttggtgtcc gccatatacc acgtccccat acggatattg ccgtcggcgg
     4441 tgtagagctg ggcggcatcc ataccgattt tgccggcgat ttcgcgcgcg gtggcaggca
     4501 taacctgcat cagcccctgc gcgcctacgc gggattgcgc gcctataacg aagcggcttt
     4561 cctgacgaat caggccgtaa acccaagccg gatcgacatt aacattttgc gcgtggcgga
     4621 ttacggtgtc tttaaacggc gaaatgtagc gcaaggtgta gttgagtttg cggtcggtgc
     4681 gttccgcgct gttgaccgcc atatcgtaaa aaccgtggtc gaacgcggtt tgcgcggcgg
     4741 ccagcagctt gtcttcgtca aagccgcgtg tggcaaaacg ccattccgcc tgagcctgac
     4801 ggcgcatttt cgcatcgccg gcggttcggc tgtgttggaa caacaccagt gcgcgcttaa
     4861 tcgcgccgtc ttccgccata cggaggacgc tgtttgtgtc ggcatcggga acattgttgc
     4921 gcgtatcgat tttgcgcccc aactcttccc ctgccagtac ggcataaaaa ttcctgcccg
     4981 tcgctgccgc ctgtttgtaa agtttttccg cttcttgcgt gttgcccgtt gcggcgcggc
     5041 tgcgtgccag ccagtagagc caggtcgggc ttttttgcag tttttcgggc atatgcgaga
     5101 tcacggaggc cagctcgtcc caacgtcggg cgcgcaaggc ggcgcgggcg taccactcga
     5161 tttggtcgtc ggtcagttgg cggcggtcgg caaccttgcc gtaatagtcc aaggcggcag
     5221 gcacattgag gctttgcgac tgataatgcc ccaatacgcc ccacgcgaaa ctgcgttgtt
     5281 cggggcttaa accgctttcc atttcggaca gcagggcggc ggcattcggc gatttgcgtg
     5341 cttctttgcc gatgacgttc aacagggcat attcgcgcga accttgtgta ccgccgtcaa
     5401 acgggctgcc caatgcggcg gcaaggttgc gtgcgtctgt ggtttggcgg ccggccagca
     5461 gtccgcgcac gcgcctccag gcatcgttgc cgtccaacaa gccggatgcg gctgcctgtt
     5521 ccaacagttt ggtgcagccc gaaggcagtt tgcccgtatt tttaactagt tcggcggcac
     5581 gcgtatagtc gttgcggctc gaatcggcgt agcattcgac ttcttgggcg cgcccttcag
     5641 gtttgagttt ggcatattcc tgtgcaaaca gcgtccactg tctgcgcgcg cccaaagact
     5701 tcagccactc gttgcggaca ttttccgcca tcgcgctgtc gccggcgttt tccaaatagg
     5761 cggcggctgc cgcatcgttg ttctgtttca ctgcatccag tgcggacggg tagccgccgt
     5821 aatctgccag cgtttttcct tccggttcgg cagggcgggt gggaacgctt gccgaaaggt
     5881 cggcagtttc tatattgtct gccggggtct tgccggctgg cagtgtgttt gtcgaagaac
     5941 acgcggcaag caccagggcc gccagcagcg gcagggaatg cttcatagag ggtaggtaca
     6001 tcggatttcc ttaagaatcg gaaccctgaa cggtcagggt tggaaaagac aaaatgccgt
     6061 ctgaacaggc gtttgcccga attatatgcc gaaactgcac cgtctttgga atgtttccga
     6121 cataatttat atttttcaat catttgccgt ttgggtgcga accgctgcct ttgcccgttt
     6181 cagacggcat tgtccgaaat ggttgcccgc ttcctgcctt attgacaaaa aaacgctttc
     6241 ccgataatat cctacgaaaa ttaacctgcc gatttgacac agcttgcggg cataacagct
     6301 aaagcgttcc gacaatttca gctttatctt ccgcgcccgt tgtgtccgac atcgggcttt
     6361 gttgtatggg aaagacaatg attattttgg acaaggtttc caagcattac caaacgcgcg
     6421 acaaaacccg ttttgccgcc gtcgagccga ccagcctcga aattcaagac ggcgaaatct
     6481 tcgggctgat gggctattcc ggcgcgggca aatccacttt gttgcgcctg atcaacctgt
     6541 tggaacgccc cgacacgggc aaagtcaacg tctgcggaca agaactgacc gcgctcgatg
     6601 ccgccgcatt gcgtcaggcg cggcagaata tcggcatggt gtttcagcag ttcaaccttt
     6661 taagcaaccg caccgtcgcc ggcaacgttg cctttccact ggaaatcgcc ggatggccgt
     6721 ctgaaaaaat caaagcgcgc gttgccgagt gcctcgaaat cgtcggcttg accgaacgcg
     6781 ccggccacta ccccgcccag ctttcaggcg ggcagaaaca acgcgtcgga atcgcccgcg
     6841 cgctcgcgcc caagccgcaa gtcatcctcg ccgacgaacc cacttccgcc ctcgaccccg
     6901 ccaccacgcg cagtgtcttg gaatgtttgg aagacatcaa caaacgcttc aacgttacca
     6961 tcgtcatcgt aacccacgaa atgagcgtca tcagacgatt gtgcgaccgc gccgccctgt
     7021 tggataaagg gaaagtggtg gaaatcgtcg aagtacgcgg caaccaaatc cacgctcaat
     7081 ccgaaatcgg gcgcgaactg attcgggagg actgatatgg cagacttaac attcgaacaa
     7141 gccgtttcca ccatcgtcgg catgaaagac gaaatcgtcc gcgccttggg cgaaactttc
     7201 gtgatggtcg gcttgtccac cacattcgcc gtcatcttcg gcacgctctt gggcgtgttg
     7261 ctcttcgtaa cttccagccg ccaactgcat tacaacaagc cggtgaactt cctgctcgac
     7321 aacctcgtca acctcatgcg cgccttcccc ttcgtcatcc tgatgattgc gatgataccc
     7381 gccacacgcg ccatcgtcgg cagcaccatc ggcccggttg ccgcctcgct ggtgttgagc
     7441 gtgtcgggat tgttttattt tgcccgactg gtggaacaaa acctgcgcga agtcccaaaa
     7501 ggtgtgattg aagccgccgc cgcgatgggt gcgccgccga ttgccatcgt cctcaaagtc
     7561 ctcttgaacg aagcgcgcgc gggcatggtt tccagcatta ccgtgcttgc catcgggctt
     7621 ttgtcataca gcgcggcggc aggcatgata ggcggcggcg gcttgggcga cctcgccatc
     7681 cgctacggct actaccgcta ccaaaccgaa gtcatcgtct tcatcgtcgc cctcctcgtg
     7741 ctgctggtca tcctgattca aagcaccggc aacgcgttgg cgcggaaact cgacaaacgt
     7801 taaacccgaa tgccgtctga acgccaaaaa ccccgccgct atccgaaaaa tgctataaaa
     7861 tccccctgtt cgcggcaaat gccgtctgaa cgccgaatcc ggacggcagg actccctgcc
     7921 tgtcattttt gtttgaaacc gccacaacat caggagaaaa tatgaaaacc ttcttcaaaa
     7981 ccctttccgc cgccgcactc gcgctcatcc tcgcagcctg cggcggtcaa aaagacagcg
     8041 cgcccgcagc ctctgccgcc gccccttctg ccgataacgg cgcggcgaaa aaagaaatcg
     8101 tcttcggcac gaccgtcggc gacttcggcg atatggtcaa agaacaaatc caagccgagc
     8161 tggagaaaaa aggctacacc gtcaaattgg tcgaatttac cgactatgtg cgcccgaatc
     8221 tggcattggc ggagggcgag ttggacatca acgtcttcca acacaaaccc tatcttgacg
     8281 atttcaaaaa agaacacaac ctggacatca ccgaagcctt ccaagtgccg accgcgcctt
     8341 tgggactgta tccgggcaaa ctgaaatcgc tggaagaagt caaagacggc agcaccgtat
     8401 ccgcgcccaa cgacccgtcc aacttcgcac gcgccttggt gatgctgaac gaactgggtt
     8461 ggatcaaact caaagacggc atcaatccgc tgaccgcatc caaagccgat atcgcggaaa
     8521 acctgaaaaa catcaaaatc gtcgagcttg aagccgcaca actgccgcgc agccgcgccg
     8581 acgtggattt tgccgtcgtc aacggcaact acgccataag cagcggcatg aagctgaccg
     8641 aagccctgtt ccaagagccg agctttgcct atgtcaactg gtctgccgtc aaaaccgccg
     8701 acaaagacag ccaatggctt aaagacgtaa ccgaggccta taactccgac gcgttcaaag
     8761 cctacgcgca caaacgcttc gagggctaca aataccctgc cgcatggaat gaaggcgcag
     8821 ccaaataagg cagtcgtata aaatgatgcc gtctgaaccg cattccgtgt tcagacggca
     8881 tttttgtcct ttaatccgcc attccctgcc attccgccaa atccggcgta tcgattccga
     8941 acagcgacag cgtgtgtgcg atactgtgcg ccactatgtc gtccgccgtc tgcggcttgc
     9001 ggtacatcgc aggaacaggg ggaaacacca cgccgcccat ttccgttacc cgcttcatat
     9061 tgtccaaatg ggcaaggttc agcggcgttt cgcgcaccat cagcaccagc cgccgccttt
     9121 ccttcaaaac cacatccgcc gcacgcgtca agagattgtc gccgaagccg tgcgcgacag
     9181 aggcaagcgt ccgcatcgaa cagggtgcga ccagcatccc gtccgtttta aacgtaccgc
     9241 tggcaatgca cgccccgata ttgccgatcg gatggacgaa atcagccaag gcatatactt
     9301 cgtctttcgt ataatccgtt tccgaagcgc gcgccatctc cgcgcctttc gataccacaa
     9361 ggtgcgtttc gacatcttgc gcgcgcaaaa gttccaaagc cttcacgccg tattggaaac
     9421 cgctcgcccc gctgatgccg attatcaaac gccgtaccat catccgcctt tcccataaaa
     9481 ccgcctgcaa cggcaaaccg gctattatag tgaaaaaaca gaaatccgat aaacacggat
     9541 acaaattgtc ggcaacaccc aatatccgat aaaatacccg atttaacatc ctatctgaat
     9601 aggcacggga gggcggtatg gcaaaagtaa aaggcggatt ggggcgcggc ttggattcgc
     9661 tgctcgccaa cggtgcggac aacagcagcg gcgaccggct gactgcggtt gcggttaaag
     9721 atatccggcc cggccgctat caggcgcgcg tccaaatcga tgacgaagcc ttgcaggaac
     9781 tggcagattc gattaaggcg caaggcgtga tacagcccgt catcgtgcgc gaacacggac
     9841 tgtcccgata cgaactgatt gcaggcgaac gccgttggcg cgccgctcag attgccggcc
     9901 tgtccgaaat ccccgccgtt atcaaaacca tcagcgacga aaccgcattg gcaatgggtt
     9961 tgatcgaaaa cctccagcgc gaaaacctca accccatcga agaagcacaa ggcttgaaac
    10021 gccttgccga cgagttcggg ctgacccacg aaaccatcgc ccaagccgtc ggcaaaagcc
    10081 gaagcgcgat ttccaacagc ctgcgccttt taagcctgcc cgaatccgtg caggaaatgc
    10141 tttaccaacg ccgcctcgaa atggggcacg cccgcgcact gctgaccctg cccgtcgtcg
    10201 aacagctcga attggcgcaa aaggccgtca aaaacggctg gtcggtgcgc gaagtcgaac
    10261 gccgcagcca ggccgccctt caaaacaaac gtcccgagcc aaaaaaaact gccgccgccg
    10321 acatcggccg cctgaatgat ttgctgactg aaaaattggg tgtcaacgcc gaaatcaaga
    10381 ccgccaacca taaaaaaggc aaaatcatcc tgcatttcga cacgcccgaa accttcgacc
    10441 atatcttgaa gcagttgggc atagattacc ggccttaatt ttgcgggata tgccgtttga
    10501 aatatagaga atagctttcc agattttaag tgggaaatat aattctattg acattttttc
    10561 tgcttcgcgt aagaatcgtt ttcctgtttt catttttaat tttcgaggaa attatgaaca
    10621 cacgcatcaa ctttaccgca actgtgggta agcgcgtgag ccaatgctat gttaccagtg
    10681 taatcagcac aatcggcgtt accacttccg atgcaatttg ttcgggaggc ggaacgaaca
    10741 aagccaaaag tcaatgcaat gctttgctta aagcggcagg ccgctgctaa tcctttattc
    10801 ggaaaaggtc gtctgaaaat attttcagac gaccttttat ttattgagca aactccccaa
    10861 actgccgaca atccgaccga taaactacta caatttcgcg ttgatttttt attatttcca
    10921 cgtaaagcaa acaaatcacg taagaacatt aaaagtgtaa agaaattgcg gacaaactcc
    10981 ttaatatcct gtaatcatat gtaatatgtg ttagaatagc ggagttggat tttggggagt
    11041 cgctcgtctg tatcggcggc gagtttcaca aagattaaca tattgcttga cgtaataagg
    11101 cttctaaatt atattagccg cgccccaaac ctgaggcgga aacattccct ttatgaagca
    11161 gattatcatc ctacaatccg ccgtattgtc catatgcgcc gcagttgcct ttgccgtatg
    11221 gggttttgcc ggattccttt cggcggtcgg gggcggtttg tcctacctgc ttcccacgtt
    11281 tgttgcagtt ttacttctaa aacttttcag gggaaacccc ttcctgcaaa gcaggatgtt
    11341 cgtcttcgga gagattttaa aagtagtgct gtcgctgttg tccatgcttg ccgtatttgc
    11401 aatatggcat caatcgctgg tgttcgtccc gtttttgatg gggctgctcg gtgtcagcca
    11461 tctggttttt ttagtattgt tgagagtgaa agattatggc aggtgaaacc attaccgctg
    11521 ccgactacat caagcaccac ttgcagagct tgaccagttt gtcggatgtt actcagggtc
    11581 agggactgaa aaacattgct gatttttcgt ttattaacct tgatgccgtt ttttttgccg
    11641 tcctgttggg cgtaatcggc agcttcctgt tgtggcgcgg tgcaaaaaaa gcaacggcag
    11701 gcgttcccgg acgtttccag gccgccgtgg aaatcttgtt cgagtttgtg gacgatatgt
    11761 gtaagagcat cattcacagc gaaaagtcta gaaaagccgt cgcgccgttg ggtctgacgc
    11821 tgtttgtttg gattttcctg atgaacgcga tggatatgct gccggtcgat ttgctgccga
    11881 tggtatggca gggcattacc ggcaaccatc acgccctgct gcgcatcgta ccgactgccg
    11941 atttgaacac cgctttggca ctcgctgtcg gcgtgttgct gatttgtatc tattacaaca
    12001 tcaaaatcaa aggattgggc ggctggtttc acgagttgtt cagtgcgccg ttcggtgcaa
    12061 aactcgcacc tgcgaacttt ctgttgaact tggtcgagtt tctttctaaa accgtatccc
    12121 acggtatgcg gttgttcggc aatatgtatg cgggcgagct ggtattcctg ctgatagcct
    12181 tgcttggcgg tgcttgggcg gcttccggca gcgttgaagt catggatccg attctgtttg
    12241 tattccacat tattgccggt ttggcatggg cgattttcca tattttggtg attaccctgc
    12301 aggcatttat tttcatggcg ttggcgttcg tctatatcgg gcaggcgcac gatgcccact
    12361 gattttccca agtagtttgt ttttgtagtt taacctttgt ttctttaagg agtttaaaat
    12421 gggtttgatt gctatcgcat gtggtttgat cgttgcattg ggtgcattgg gtgcatctat
    12481 cggtatcgca atggtcggtt ctaaatattt ggagtcttct gctcgccaac ctgaactgat
    12541 tggtccgctg caaaccaaac tgttcctgat tgccggtctg attgatgccg cattcttgat
    12601 cggtgtcgcc attgcactac tgttcgcctt cgtcaacccg tttgcaggtg cataatcagg
    12661 acggattcgt ccgttcgtac aggtgcagcc tgtttgatta accccaatac gaaggttaag
    12721 taacgtgaat atcaatgcaa cattattcgc tcaaatcatc gtctttttcg gtttggtatg
    12781 gtttaccatg aaatttgtgt ggccgccgat tgcaaaagct ttggatgagc gtgccgcaaa
    12841 aatcgccgag ggcttggctg ccgccgagcg tggtaaaagc gatttcgagc aggctgaaaa
    12901 aaaggttgca gaacttttgg cagaagggcg taatcaggtt tccgaaatgg ttgccaacgc
    12961 cgaaaaacgt gccgccaaaa ttgtcgaaga agccaaagaa caggcttctt ccgaggcggc
    13021 gcgcattgca gctcaggcaa aggccgatgt ggagcaggaa ttgttccgcg cacgcgaatc
    13081 cctgcgcgat caggttgccg tgttggctgt caaaggtgcc gaatctattt tgcgcagcga
    13141 agtcgatgct tccaaacacg caaaactgct cgataccctg aaacaggagt tgtaatctta
    13201 tggcagagtt tgcaacgatc gccagacctt atgcaaaggc attgttcggt ctggctcagg
    13261 aaaaaaacca aattgagtct tggttgggcg gactggaaaa acttgcggcg gttgttcagg
    13321 aagggaaggt ggctttattg attgaccgtc ctgaaacgaa tgcttcagaa aaagcagata
    13381 tcctcatcga tttggtcggt ttgaaagaca aggagttaaa aaactttgtt atcgtcttgg
    13441 ccgggcagaa acgtttgtcg atattgccgg aagtgtatgc tcaatatcaa gacttgacct
    13501 tatcattcaa ccatatcaaa tctgccgtca tttacagtgc ctatccgttg accgacaaac
    13561 aggtcggcga gttggcgcaa atgctgaata agcgtttcga cagcgagctg aaaatctctg
    13621 tcgaaatcga accggagctg attggcggca taaaagttga agtgggtgat caggttttgg
    13681 atttgtctgt acaaggcaaa ctgagtgctt tgtacacgac tatgacgaat taggagagtt
    13741 ttcatgcagc ttaatcctgc tgaaattagc gatctgatta aagccaagat cgaaaatctg
    13801 tctgtaaatg ccgaagtgag tacccgtggt acggtgattt cggtaactga cggtatcgtt
    13861 cgcatccatg gtttgtcaga tgcaatgcaa ggtgagatgc tcgaattccc gggtaacact
    13921 ttaggcttgg cgatgaacct ggagcgcgac tccgtcggtg ccgtagtgtt gggcgagtac
    13981 gaacatatta aagaaggcga cacggttacc tgtaccggcc gtattttaga agtgccggtc
    14041 ggacgcgaat tggtcggacg cgtcgtcgat gcattgggtc ggcctatcga cggcaaaggc
    14101 ccgattaata caactttgac cgcccctgtc gaaaaaatcg caccgggcgt gattgcacgc
    14161 aaatcggttg atcagccgat gcaaaccggt ctgaaggcga ttgactctat gattcctgtc
    14221 ggtcgcggtc agcgtgagtt gattattgga gaccgtcaga caggtaaaac ggccgtggca
    14281 ttggatgcca tcgtcaacca aaaaggtacg ggtgttatct gtatctatgt cgctatcggt
    14341 caaaaagcat cttctattgc caacgtggtc cgcaaattgg aagagcatgg cgcgatggaa
    14401 cacacgattg tggttgctgc aactgcatct gaagcggcgg cattgcaata tatcgcacct
    14461 tattcaggtt gtacgatggg tgaattcttc cgcgatcgtg gcgaagatgc cttgattgtt
    14521 tatgacgatt tgtccaaaca ggctgtggct taccgtcaaa tttccctgct tttgcgccgt
    14581 ccgcccggcc gcgaagccta tcccggcgat gtgttctacc tgcactcccg tctgttggaa
    14641 cgtgcggcac gtgtcaatga acacgaagtg gaaaaattga ccaacggcga agtaaaaggc
    14701 aaaaccggtt ctctgaccgc gttgccgatt atcgaaaccc aagcgggcga cgtatctgct
    14761 ttcgttccga ctaacgtcat ttcgattacc gacggtcaga ttttcttgga aaccgacctc
    14821 ttcaacgccg gtatccgtcc tgcaatcaat gccggtattt ccgtatcccg cgtaggcggt
    14881 gctgcacaaa ccaaagtgat taaaaagctg ggtggcggta tccgtttggc gttggcacaa
    14941 tatcgtgaat tggcggcgtt ctcgcaattt gcatccgatt tggatgaagc tacgcgcaaa
    15001 cagttggagc atggcgaagt cgtaaccgaa ctgatgaaac agaaacagtt cagtacgttg
    15061 aatacggctg aaatggcttt gaccctttgg gcaatcaaca acggttcgta ttctgatgtt
    15121 ccggttgcca aagctctagc ttttgaatct gaatttttga gctttgtccg tacccaacat
    15181 ccggaagttt tggaagccgt caatgcttca ggtgcaatgt ccgacgagag cgagaaaacg
    15241 cttgaagcag ccatgaaatc cttcaaatct tcttacgcct atcaggcata aggcttgaag
    15301 tgaaaggagt ctgaaatggc agtaggaaaa gagattctca ccaaaatccg cagtgttcag
    15361 aatacccaaa agatcactaa agcgatgcaa atggtgtcaa cctctaaaat gcggaagact
    15421 caggaacgga tgcgtttggc gcgtccgtat gccgaaaaag tgcgtatggt gatgagccat
    15481 cttgcgcaaa ccaataccga tcatggtatt ccgttactgg aatctcatcg ggaaatcaga
    15541 cgtgtcggtt ttattttgat tacgtctgat aagggtttgt gtggcggttt gaacgccaac
    15601 gtgctgaaaa agtttttggc acaagttcaa gagtatcgga atcaaggtat tgaagaggaa
    15661 atcgtatgcc ttggcagtaa aggtctgatg gcgtgtcaga gcattggtct gaatgtggtt
    15721 gccagtgccg taaatttggg cgatacccca aaaatggaaa tgctgctcgg acctttgaca
    15781 gaacttttcc aacggtatga gaaacatgaa attgacaaaa tccatctggt gtattcgggt
    15841 tttgtcaata ccatgcgtca agaaccgagg atggaagtat tgctgcctat cggtgagaac
    15901 gtgattggcg attcagctcc caaaccaccg ttcagctggg aataccgcta cgaaccgact
    15961 gcacttgcag tgttggaata tctggttcgc cgctatttag agtctgtggt ttatcaggcg
    16021 ttgagcgaca atatggcatc cgaacaggca gcccgcatgg ttgccatgaa agctgcaaca
    16081 gacaatgcag gcaatgccat caaagagttg cgtctggtat ataacaaatc gcgtcaagct
    16141 gcgattacca cggaattgtc agaaattgta gcaggtgcgg cggccgtctg atgccgtctg
    16201 aatcctgtac ggaaattagg atacgataat gagccaaggc aaaatcgtac aaattatcgg
    16261 tgcggttgtt gacgtggaat ttccacgcga catgattccg cgcgtttacg acgctttgaa
    16321 attagacgaa aacggtctga ctttggaagt ccaacagctt ttgggtgatg gcgtagtccg
    16381 taccatcgct atgggcagct cggacggttt gaaacgcggc atgactgtga gcaatactgg
    16441 ttcgcccatt actgtgccgg taggtaaagg tacgttgggg cgcattgtcg atgtattggg
    16501 aactcctgtt gacgaggcag gtccaattga taccgacaag agtcgtgcca tccaccaagc
    16561 cgctcctaag tttgacgaac tgtcttccac aaccgaattg ctcgaaacgg gcattaaagt
    16621 gattgacttg ctgtgtccgt ttgccaaagg cggtaaagta ggtctgttcg gcggtgccgg
    16681 tgtgggtaaa accgtgaaca tgatggaatt gatcaacaac atcgccaaag cgcacagcgg
    16741 cttgtccgtg ttctcaggcg tgggtgagcg tacccgcgaa ggtaacgact tctaccacga
    16801 gatgaaagat tccaacgtat tggataaagt agccatggtg tatggccaaa tgaacgaacc
    16861 tccaggcaac cgtctgcgcg ttgctttgac cggtttgact atggctgaat acttccgtga
    16921 cgaaaaagac gaaaacggta aaggtcgtga cgtattgttc ttcgttgaca acatctaccg
    16981 ttacactctg gccggtaccg aagtatccgc actgttgggc cgtatgcctt ctgcagtggg
    17041 ttaccaaccg acattggctg aagaaatggg tcgtttgcaa gagcgtatta cctctaccca
    17101 aaccggttcc attacttcca tccaagccgt atatgtacct gcggatgact tgactgaccc
    17161 gtctccggcg acaactttcg cccacttgga cgcgaccgtc gtattgagcc gtgatattgc
    17221 ctctttgggt atttacccgg cagttgaccc gcttgactct acttcgcgcc aattggatcc
    17281 gatggtattg ggtcaagagc actacgacgt agcgcgcggt gtacagtcta ctctgcaaaa
    17341 atacaaagaa ttgcgcgaca tcatcgccat tctgggtatg gacgaattgt ctgacgaaga
    17401 caaactggct gtaatgcgtg cccgtaaaat ccaacgcttc ctgtctcaac cgttccacgt
    17461 tgccgaagtg tttacaggtt ctccaggcaa atatgtcgcc ctgcgcgata ccattgccgg
    17521 cttcaaagcc atcttgaacg gcgaatacga tcatctgccc gaacaggcat tctatatggt
    17581 cggcagcatc gaagaagcgg ttgagaaagc gaaaacctta aactgaggag gtcggcatga
    17641 gcatcatgca agttgaggtg gtaagtggcg agcagaaaat ctattcaggc gaggcaacat
    17701 ttatcgttgt tccgactgta cagggcgaac tcggtattta tccgcgacac gagccgatta
    17761 tgagtttggt gcggccgggg gctttgcgtt tgaccgttcc gggcgaggat aaagaggttt
    17821 tggttgctgt ttccggcggt attttggaag tacagcctga taaagtaact gtcttggcgg
    17881 atgttgccgt ccgcagtgcg gaaatggatc gggcacgtgc ggaagaggcg aaaaaagccg
    17941 cagaagcggg catttcccaa gctaaagacg ataaggcttt ggcggaagca cataaagcat
    18001 tggctgctgc gattgcgcag ctcaaaactt tggactatat ccgttcgcac aagaaataaa
    18061 cttgtttggt ttgaaaaagc acgaccgttt ggccgtgctt tttattttcc ggatttaaat
    18121 taaaaaatta tccatcccct ggtttcaaat caggctgtct tcgagataaa tctattcaag
    18181 cggctatgga aacagtaaaa taaaaccttt attttatcga acaataagcc atgcttacct
    18241 tccaacaaat catcttcaaa ctgcaaacct tctgggcaga caaaggctgc accgtcatcc
    18301 aacccttcga catggaagtc ggtgccggca catcccatcc cgccacctgc ctgcgcgcgc
    18361 tcggccccga gccttggttt gccgcctacg tccaacccag ccgccgcccc aaagacggcc
    18421 gctacggcga caaccccaac cgcctgcaac actattacca attccaagtc gccctcaaac
    18481 ccgcccccgc caatatccaa gacctctatc tcgactccct gcgcgaattg ggcatcgatc
    18541 ccaaagtcca cgacatccgc tttgtcgaag acgactggga aaaccccacc ctcggtgcgt
    18601 ggggtttggg ctgggaagtc tggctcaacg gcatggaagt aacccagttt acctatttcc
    18661 aacaagtcgg cggtatcgat tgcacgcccg tactcggcga aattacctac ggcatcgaac
    18721 gcttggcgat gtacctgcaa ggcgtggaaa acgtctatga cctcgtttgg gcaaaaacgc
    18781 cggacggcaa taccgtaagc tacggcgacg tgtaccatca aaacgaagtc gagcaatcta
    18841 cctacaactt cgaatacagc gatgccgact ggctgctgcg ccagttcaac gactacgaag
    18901 cgcaagccaa acgcctgttc gccgaagaaa acgccgacct tgccttacct gcctacgagc
    18961 tggtcctcaa agcggggcat acgttcaacc ttttagacgc acgcggcgcg atttccgtaa
    19021 ccgagcgggc aacttatatt ggacgtattc gtgcattgag ccgcgccgtg gcgcaaaaat
    19081 atatcgaaag ccgtgagaaa ctgggcttcc cgttgattaa aaaataacga tacgtcgtct
    19141 gaagcaggtt ttcaggcgac ctttattgcg tatccattaa tacaggccgt acggcatatg
    19201 caggctttat aagcatggcc tacggacaaa cagaaagtct cacatgacaa cccaaaccct
    19261 tttaatcgaa ctccttaccg aagaactccc gccaaaagcc ctgaataatc tgggcaacca
    19321 ttttgccgct tccgttgccg aaggcttgga aaaagcgcaa ctggttgacg gcgcggccga
    19381 atttacggct tatgcctcgc cgcgccgttt ggcggttcaa gtcaaaaacg tgaaggccgt
    19441 tcaagccgat cagaaaatcg tgaaaaaagg ccctgccgtg gcgaatgccg taaaagacgg
    19501 tgcgccgacc aaggctttgg aaggttttgc gcgcggcgca ggcgcgaaaa tcgaagacct
    19561 gaccatcgtc cacgacggca ggcaggacgt gtacgcctat gaatacgtcc aaaccggcaa
    19621 accgttgggc ggacttttgg aaaacattat caatcaagct gttaagaagc tgccgattcc
    19681 gaaagtaatg cgttggggca gcagcacgtt taccttcgtg cgccctgttc acgggctgat
    19741 tgtgctgcac ggcggcgatg tcgtgaacgt cagcgttttg ggcctgcaaa gcggcaatca
    19801 aaccttggga caccgctttc tttccgacgg cgaaatcatc attgaaaacg ccgacagcta
    19861 cgccgcacaa atgcgcgggc aaggcaaagt cgtcgcttcg tttgccgggc gcaaagccgc
    19921 gattcagacg gcattggaag ggcaggcacg ccgtctgaac gcgaccgttg ccgccgatga
    19981 agccctgttg gacgaagtga ccgcgctggt cgaatggcct gttgttttgg aagccggttt
    20041 tgaggaacac ttcctcgccg tgcctcaaga atgcttgatt ttgacgatgc agcaaaacca
    20101 aaaatacttc ccgctgctcg accaaaacgg caagctgatg aaccgcttcc tgctggtttc
    20161 caacctgcaa accgaagacc cgtcgcacat catccgaggc aacgaacgcg tattgcgcgc
    20221 gcgcctgtct gatgccgagt tcttctacaa acaagaccaa aaagcgacct tggaaagccg
    20281 cctgcccaag ctggcgaacg tggtgtacca caacaaaatc ggttcgcagg ccgaacgcat
    20341 cgagcgtctg caaagcatcg ccgcccacat cgccaaagca ttgggcgcgg atgccgcggc
    20401 agccgggcgc gccgcgcgtc ttgccaaagc cgacttggta actgaaatgg tcggcgagtt
    20461 ccccgaactg caaggcacga tgggcaaata ctacgcccgc ttggacggcg aaaccgaaga
    20521 aatcgccgaa gctattgagc aacactacca accgcgtttt gccggcgaca agctgcccga
    20581 aagcaaaatt gccgccgccg tggcacttgc cgacaaactg gaaaccctgg tcggcatttg
    20641 gggcatcggt ttgattccta ccggcgacaa agacccctac gccctgcgcc gcgccgcctt
    20701 gggtattttg cggatgctga tgcagtacgg tttggacgtg aacgaactga ttcagacggc
    20761 attcgacagc ttcccgcaag gtttgctcaa cgagaaaacg ccgtctgaaa ccgccgactt
    20821 tatgcaggcg cgccttgccg tgttgctgca aaacgattat ccgcaagaca tcgttgccgc
    20881 cgtactcgcc aaacagccgc gccgtttgga cgatttgacc gccaaactgc aagccgtcgc
    20941 cgtattcaaa caactgcccg aagccgccgc gctcgccgcc gccaacaaac gcgtgcaaaa
    21001 cctgctgaaa aaagccgatg ccgagttggg cgcggttaac gaaagcctgc tgcaacagga
    21061 cgaagaaaaa gccctgtacg ctgccgcgca aggtttgcag ccgaaaatcg ccgccgccgt
    21121 cgccgaaggc aatttccaaa ctgctttgtc cgaactggct tccgtcaagc cgcaggttga
    21181 tgccttcttc gacggcgtga tggtgatggc ggaagatgcc gccgtaaaac aaaaccgcct
    21241 gaacctgctg aaccgcttgg cagggcagat gaacgcggtg gccgacatcg cgcttttggg
    21301 cgagtaaccg ttgtacagtc caaatgccgt ctgaagcctt caggcggcat caaattatcg
    21361 ggagagtaaa ttgcagcctt tagtcagcgt attgatttgc gcctacaacg tagaaaaata
    21421 ttttgcccaa tcattagccg ccgtcgtgaa tcagacttgg cgcaacttgg atattttgat
    21481 tgtcgatgac ggctcgacag acggcacact tgccattgcc aaggattttc aaaagcggga
    21541 cagccgtatc aaaatccttg cacaagctca aaattccggc ctgattccct ctttaaacat
    21601 cgggctgga
//
LOCUS       DAWTQS010000032        20911 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.032, whole genome shotgun sequence.
ACCESSION   DAWTQS010000032 DAWTQS010000000
VERSION     DAWTQS010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20911
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.032"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(2..466)
                     /locus_tag="WNA25_001180"
     CDS             complement(2..466)
                     /locus_tag="WNA25_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823938.1"
                     /translation="MKRFILPVLLSATTALASPIVGTWHCIGTDENIHSDTKVKYLQD
                     GSFRGDAILKIDDDGNILAYRVVGEGKWRFANNALTQSQIKYGEVSRQHSPETLAWLE
                     KSEDARLLESMMYTGLVAQMDKPGKDDVYQLDKSGKLVSEDGTSREACTKVE"
     gene            complement(543..3380)
                     /locus_tag="WNA25_001181"
     CDS             complement(543..3380)
                     /locus_tag="WNA25_001181"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFA9823939.1"
                     /translation="MLDKYSPAEIESKHYQNWESQGYFRPDMDLTKPSFSIQLPPPNV
                     TGTLHMGHAFNQTIMDGLTRYYRMKGCNTAWIPGTDHAGIATQIVVERQLAAQNVSRH
                     DLGREKFLEKVWEWKEVSGGTITQQMRRVGCSADWTREYFTMDGVRAETVTEVFVRLY
                     EQGLIYRGKRLVNWDPVLGTAVSDLEVESMEEQGSMWHIRYPLADNPTEAVIVATTRP
                     ETLLGDAAVAVNPEDERYTHLIGKELILPLTGRTIPVIADEYVEKDFGTGCVKITPAH
                     DFNDYEVGKRHDTRLINVFDLEAKVLANAEVFNFKGEAQPGFSLPEKYAGLDRFAARK
                     QMVADLQEQGFLVEIKPHTLMTPKGDRTGSVIEPMLTSQWFVAMSATPNGGEPDNEFK
                     GLSLADKAKKAVDSGAVRFIPENWVNTYNQWMNNIQDWCISRQLWWGHQIPAWYDEAG
                     NVYVARNQAEAEKQAGKTGLTREEDVLDTWFSSALVPFSTLGWPSETDELKAFLPSNV
                     LVTGYEIIFFWVARMIMMTTHFTGKVPFKAVYIHGIVRDHEGKKMSKSEGNVIDPVDL
                     IDGIGLDKLLMKRTTGLRKPETAPKVEEATKKLFPEGIPSMGADALRFTMASYASLGR
                     SVNFDFKRAEGYRNFCNKIWNATNFVLMNTENQDCGYGATAAEPRGHSFPDMWIIGRL
                     NQTIEQVTQAYETYRFDLAAETLYSFVWNDYCDWYLELAKVQLQTGCASRQRATRHTL
                     LRVLEAALRLLHPIIPFITEELWQTVAPMCDAKTADSIMLARFPETDGGEIVQTAFGQ
                     MTVLQDLIGAVRNLRGETGIQPNVKAPLFVESADDLADYLKYLPMMTRLTEARQVAAL
                     PESGDAPVAVCNGARLMLKVEIDKAAETARLSKEAEKLQKALDKLNAKLSKPGYTEKA
                     PAHLVEKDKADLAELEDKMAKVQNQLAKLKD"
     gene            complement(3485..4744)
                     /locus_tag="WNA25_001182"
     CDS             complement(3485..4744)
                     /locus_tag="WNA25_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFA9823940.1"
                     /translation="MKVLVLGAGVAGVSSVWYLAEAGHEVTVIDRTEGVAMETSFANA
                     GQLSYGYTTPWAAPGIPTKALKRLFKSHPPLLFRPDGGLYQIEWLWRMLQNCTATRYQ
                     INKERMVRISEYSREMFRRFEAQTDMNFEGRKKGTLQIFRQTEEVEAAKQDIAVLERY
                     GVPYRRLKPEECAEFEPALARVTAKIVGGLHLPADATGDCRLFTENLYKLCQEKGVRF
                     YFNQTISRIDHNGLRIKAVETETGRFETDAVVCALGCFSRTVLAQLDLNLPIYPVKGY
                     SLTLPVTNSDGAPVSTVLDESYKVAITRFDNRIRVGGMAELSGYETKLPEKRRETLAL
                     VVNDLFPEGGDLSQALSWSGLRPMTPDSTPLIGRTRFENLFLNTGHGTLGWTMSPGSA
                     KLTADIVSGKDTEIRSDDLSLSRYQKL"
     gene            complement(4823..6214)
                     /locus_tag="WNA25_001183"
     CDS             complement(4823..6214)
                     /locus_tag="WNA25_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HFA9823941.1"
                     /translation="MQVFLDNLKAFFETVSSWVWGSVMLMLLVGTGIVLTVMLKGLQF
                     TMLGYALKQAFVPSKKYEGGAGHEGDISHFAALMTALSATIGTGNIAGVATAVVTGGP
                     GAVFWMWMTAIFGMATKYGEGVLAVKYRVNNSKGEMSGGPMYYIEKGLGKNWKWMAVA
                     FALFGTFASFGIGSSVQSNSVAQAVQTSFGIEPAYTGITLTVLTAVVVLGGIKGIAKA
                     ASFIVPAMAVFYVLGGLSIIVINSDALMPAVKLIFSDAFSAQAVAGGAIGTVIRYGVA
                     RGVFSNEAGMGSAPIAAAAAKTDHPVRQALVSMTGTFLDTIVVCSITGIVLVMGLLGA
                     GGEFVKPELSGAALTTVTFQKMLPGIGGWIVTIGLIFFAYSTILGWCYYGEKCAVYVF
                     GEKFAALYRVGYVSSVMPGTVLSLDLVWLASDTFNGLMALPNLTALLLMAKVIVNETR
                     DFKQKITNGELPH"
     gene            complement(6476..6580)
                     /locus_tag="WNA25_001184"
                     /pseudo
     CDS             complement(6476..6580)
                     /locus_tag="WNA25_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(6677..7453)
                     /gene="lpxA"
                     /locus_tag="WNA25_001185"
     CDS             complement(6677..7453)
                     /gene="lpxA"
                     /locus_tag="WNA25_001185"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFA9823942.1"
                     /translation="MTLIHPTAVIDPKAELDSGVKVGAYTVIGPNVRIGANTEIGPHA
                     VINGHTTIGENNRIFQFASLGEIPQDKKYRDEPTKLIIGNGNTIREFTTFNLGTVTGI
                     GETRIGDDNWIMAYCHLAHDCVVGNHTIFANNASLAGHVTVGDYVVLGGYTLVFQFCR
                     IGDYAMTAFAAGVHKDVPPYFMASGYRAEPAGLNSEGMRRNGFTAEQISAVKDVYKTL
                     YHRGIPFEEAKADILRRAETQAELAVFQDFFAQSTRGIIR"
     gene            complement(7496..8176)
                     /locus_tag="WNA25_001186"
     CDS             complement(7496..8176)
                     /locus_tag="WNA25_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HFA9823943.1"
                     /translation="MERYKNAVRKDKAAELYLLNLSLSRELFHVVSIFEIVLRNKIDI
                     CFRQEFKDRNRLYDSIQPQTNPALKYQGCFLRNGTKESAELIKVALSKIQNNSGGKFD
                     HNQLVAGLGFGFWRYLFAGGKDAQFDAAGKVLMKVFPKKPKSTPSVQYNQKWIFRELS
                     NINNFRNRLAHHEPICFKGAIKDTGYARNIYQSIFELLNYMDVDTASVFSHFSDQVIA
                     VCDEIDKL"
     gene            complement(8383..8832)
                     /gene="fabZ"
                     /locus_tag="WNA25_001187"
     CDS             complement(8383..8832)
                     /gene="fabZ"
                     /locus_tag="WNA25_001187"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFA9823944.1"
                     /translation="MDVQLPIEAKDIQKLIPHRYPFLQLDRITAFEPMKTLTAIKNVT
                     INEPQFQGHFPDLPVMPGVLIIEAMAQACGTLAILSEGGRKENEFFFFAGIDEARFKR
                     QVIPGDQLVFEVELLTSRRGIGKFNAVAKVDGQVAVEAVIMCAKRVV"
     gene            complement(8868..9911)
                     /gene="lpxD"
                     /locus_tag="WNA25_001188"
     CDS             complement(8868..9911)
                     /gene="lpxD"
                     /locus_tag="WNA25_001188"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFA9823945.1"
                     /translation="MIPATCTLSQITARLGGEWRGEDISVTAVRPLADAQAEHISFLA
                     NPKYKAEVHDSSAGAIIVSAKAADGFEGRNLIVADDPYLYFAKVARLFSPVVKARGGI
                     HPTAVVEPGATVPASCEIGANAYIGANTVLGEGCRILANAVVQHDCKLGDEVVLHPNA
                     VVYYGCTLGRHVEIHSGAVIGADGFGLAFAGDSWFKIPQTGAVTLGDDVEIGSNTNID
                     RGAMSDTIVGNGTKIDNQVQIGHNCKIGSHTVIAAKTGISGSVTIGSYCIIGGGVGTV
                     GHIEIADKTTIGGGTSVTHSITESGKHIAGIFPMSTHKEWARNAVYIHRLSEMNKRLK
                     TLEQQLSDSKDTQ"
     gene            complement(9944..10444)
                     /locus_tag="WNA25_001189"
     CDS             complement(9944..10444)
                     /locus_tag="WNA25_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HFA9823946.1"
                     /translation="MTRLTRAFAATLIGLCCTTGTHADTFQKIGFINTERIYLESKQA
                     RNIQKTLDGEFSARQDELQKLQREGLDLERQLAGGKLKDAKKAQAEEKWRGLVEAFRK
                     KQAQFEEDYNLRRNEEFASLQQNANRVIVKIAKQEGYDVILQDVIYVNTQYDVTDSVI
                     KEMNAR"
     gene            complement(10510..12888)
                     /gene="bamA"
                     /locus_tag="WNA25_001190"
     CDS             complement(10510..12888)
                     /gene="bamA"
                     /locus_tag="WNA25_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFA9823947.1"
                     /translation="MKLKQIASALMMLGISPLAFADFTIQDIRVEGLQRTEPSTVFNY
                     LPVKVGDTYNDTHGSAIIKSLYATGFFDDVRVETADGQLLLTVIERPTIGSLNITGAK
                     MLQNDAIKKNLESFGLAQSQYFNQATLNQAVAGLKEEYLGRGKLNIQITPKVTKLARN
                     RVDIDITIDEGKSAKITDIEFEGNQVYSDRKLMRQMSLTEGGIWTWLTRSDRFDRQKF
                     AQDMEKVTDFYQNNGYFDFRILDTDIQTNEDKTRQTIKITVHEGGRFRWGKVSIEGDT
                     NEVPKAELEKLLTMKPGKWYERQQMTAVLGEIQNRMGSAGYAYSEISVQPLPNAGTKT
                     VDFVLHIEPGRKIYVNEIHITGNNKTRDEVVRRELRQMESAPYDTSKLQRSKERVELL
                     GYFDNVQFDAVPLAGTPDKVDLNMSLTERSTGSLDLSAGWVQDTGLVMSAGVSQDNLF
                     GTGKSAALRASRSKTTLNGSLSFTDPYFTADGVSLGYDIYGKAFDPRKASTSVKQYKT
                     TTAGGGVRMGIPVTEYDRVNFGLAAEHLTVNTYNKAPKRYADFIKQYGKTDGADGSFK
                     GLLYKGTVGWGRNKTDSALWPTRGYLTGVNAEIALPGSKLQYYSATHNQTWFFPLSKT
                     FTLMLGGEVGIAGGYGRTKEIPFFENFYGGGLGSVRGYESGTLGPKVYDEYGEKISYG
                     GNKKANVSAELLFPMPGAKDARTVRLSLFADAGSVWDGRTYTAAENGNNKSVYSENAH
                     KSTFTNELRYSAGGAVTWLSPLGPMKFSYAYPLKKKPEDEIQRFQFQLGTTF"
     gene            complement(12945..14285)
                     /gene="rseP"
                     /locus_tag="WNA25_001191"
     CDS             complement(12945..14285)
                     /gene="rseP"
                     /locus_tag="WNA25_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFA9823948.1"
                     /translation="MQTLLAFIFAILILVSLHEFGHYIVARLCGVKVVRFSVGFGKPF
                     FTRKRGDTEWCLAPIPLGGYVKMVDTREGEVSEADLPYAFDKQHPAKRIAIVAAGPLT
                     NLALAVLLYGLSFSFGVTELRPYVGTVEPDTIAARTGFQSGDKIQSVNGVSVQDWSSA
                     QTEIVLNLEAGKVAVGVQTASGAQTVRTIDAAGTPEAGKIAKNQGYIGLMPFKITTVA
                     GGVEKGSPAEKAGLKPGDRLTAADGKPIASWQEWANLTRQSPGKKITLTYERAGQTHT
                     ADIRPDTVEQPDHTLIGRVGLRPQPDRAWDAQIRRSYRPSVVRAFGMGWEKTVSHSWT
                     TLKFFGKLISGNASVSHISGPLTIADIAGQSAELGLQSYLEFLALVSISLGVLNLLPV
                     PVLDGGHLVFYTVEWIRGKPLGERVQNIGLRFGLALMMLMMAAAFFNDVTRLIG"
     gene            complement(14320..15555)
                     /locus_tag="WNA25_001192"
     CDS             complement(14320..15555)
                     /locus_tag="WNA25_001192"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFA9823949.1"
                     /translation="MPSENASDGIRYKVILIMTPQVLTILGSTGSIGESTLDVVSRHP
                     EKFRVFALAGHKQVEKLAAQCQTFRPEYAVVADAEHAARLEALLKRDGTATQVLHGAQ
                     ALVDVASADEVSGVMCAIVGAAGLPSALAAAQKGKTIYLANKETLVVSGALFMETARA
                     NGAAVLPVDSEHNAIFQVLPRDYTDRLNEHGIDSIILTASGGPFLTTDLSTFDSITPE
                     QAVKHPNWRMGRKISVDSATMANKGLELIEAHWLFNCPPDKLEVVIHPQSVIHSMVRY
                     RDGSVLAQLGNPDMRTPIAYCLGLPERIDSGVGKLDFGALSALTFQKPDFGRFPCLKF
                     AYETINAGGAAPCVLNAANETAVAAFLDGQIKFTDIAKTVAHCLAQDFSNGMGDIEGL
                     LAQDARTRAQARAFIGTLR"
     gene            complement(15560..16357)
                     /locus_tag="WNA25_001193"
     CDS             complement(15560..16357)
                     /locus_tag="WNA25_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFA9823950.1"
                     /translation="MLKQRVITAMWLLPLMLGMLFYAPQWLWAAFCGLIALTALWEYA
                     RMAGLCKTETNHYLAATLVFGVVAYAGGWMLPNLVWYVVLAFWLAVMPLWLRFKWRLN
                     GGWQVYAVGWLLLMPFWFALVSLRPHPDDALPLLAVMGLVWVADVCAYFSGKALGKHK
                     IAPAISPGKSWEGAIGGAVCVAVYMTAVRSAGWLAFDTGWFDTVLIGLVLTVVSVCGD
                     LLESWLKRAAGIKDSSNLLPGHGGVFDRTDSLIAVISVYAAIMSVLN"
     gene            complement(16360..17106)
                     /locus_tag="WNA25_001194"
     CDS             complement(16360..17106)
                     /locus_tag="WNA25_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFA9823951.1"
                     /translation="MKSSTQTILEHTAIPRHIAVIMDGNGRWAKKRFLPRIMGHKRGL
                     DALENMVKHCAKLGVQYLTVFAFSTENWRRPEDEVSFLMGLFLQALQKQVRRLHENNM
                     RLKILGSRERFNRQILQGIEEAEALTANNTGLTLSIAADYGGRWDILQAANKLIAEGV
                     SEITEDTLAKHLMLGDAPEPDLFIRTGGETRISNFLLWQMAYAELYFTDILWPDFDET
                     ALDAAVASFQKRERRFGRTSEQLPIGQQRN"
     gene            complement(17162..17719)
                     /gene="frr"
                     /locus_tag="WNA25_001195"
     CDS             complement(17162..17719)
                     /gene="frr"
                     /locus_tag="WNA25_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFA9823952.1"
                     /translation="MINDIQKTAEGKMQRSVEVLKENLAKVRTGRAHTGLLDQVEVEY
                     WGSMVPVSQVANVTLLDARTIGVKPFEGNMAAKVEKAIRDSNLGLNPAAVGDLIRVPM
                     PMLTEERRKDLIKVVRGEAEEGRVSIRNVRRDANDHIKKLLKDKEISEDEARRGEEAV
                     QKLTDKYIAEADKVLAAKEEDLMAV"
     gene            complement(17861..18853)
                     /locus_tag="WNA25_001196"
     CDS             complement(17861..18853)
                     /locus_tag="WNA25_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFA9823953.1"
                     /translation="MKIISLFSGCGGLDLGFEKAGFEIPAANEYDKTIWATFKANHPK
                     THLIEGDIRKIKEEDFPEEIDGIIGGPPCQSWSEAGALRGIDDARGQLFFDYIRILKS
                     KQPKFFLAENVSGMLANRHNGAVQNLLKMFDRCGYDVTLTMANAKDYGVAQERKRVFY
                     IGFRKDLEIKFSFPKGSTVEDKDKITLKDVIWDLQDTAVPSAPQNKTNPDAVNNNEYF
                     TGSFSPIFMSRNRVKAWDEQGFTVQASGRQCQLHPQAPKMEKHGANDYRFAAGKETLY
                     RRMTVREVARIQGFPDNFKFIYQNVNDAYKMIGNAVPVNLAYEIAAAIKKTLER"
     gene            complement(18858..19373)
                     /locus_tag="WNA25_001197"
     CDS             complement(18858..19373)
                     /locus_tag="WNA25_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoPII family restriction endonuclease"
                     /protein_id="HFA9823954.1"
                     /translation="MTACLQKPAKMRKNGKRKTLSIIVGVVDKKKNLKHLAMVYGIDY
                     CADAECYLKIKNQIKEGIGNIGGIQFAETKELGRVNRIDPLNITYLRVRGMWGIENPW
                     FVFNYIYQRNMEKSFNFMAIINEDKWNSFNNTDKLLAIQDSKLAISDIKIKNPNNPAR
                     LRNAKLITYHL"
     gene            complement(19291..19695)
                     /locus_tag="WNA25_001198"
     CDS             complement(19291..19695)
                     /locus_tag="WNA25_001198"
                     /inference="COORDINATES: protein motif:HMM:NF021068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoPII family restriction endonuclease"
                     /protein_id="HFA9823955.1"
                     /translation="MNIIDAIINLANNPVVGVNSHSQSNNRANQAGDALEEYVKDLFS
                     GSFNLNETQRIARHAKVFSYLGNNSNPPDAMLRNGDAIEVKKIESKDSALALNSSHPK
                     SKLSVDDSMLTKACKDAEKWEEKDIIYNCRRR"
     gene            complement(19804..>20911)
                     /locus_tag="WNA25_001199"
     CDS             complement(19804..>20911)
                     /locus_tag="WNA25_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFA9823956.1"
                     /translation="RLSGRWLNSYERYRHRRLIHAVRLGGAVLFATALARLLHLQHGE
                     WIGMTVFVVLGMLQFQGAIYSKAVERMLGTVIGLGAGLGVLWLNQHYFHGNLLFYLTI
                     GTASALAGWAAVGKNGYVPMLAGLTMCMLIGDNGSEWLDSGLMRAMNVLIGAAIAIAA
                     AKLLPLKSTLMWRFMLADNLADCSKMIAEISNGRRMTRERLEQNMVKMRQINARMVKS
                     RSHLAATSGESRISPSMMEAMQHAHRKIVNTTELLLTTAAKLQSPKLNGSEIRLLDRH
                     FTLLQTDLQQTAALINGRHARRIRIDTAINPELEALAEHLHYQWQGFLWLSTNMRQEI
                     SALVILLQRTRRKWLDAHERQHLRQSLLETREHG"
BASE COUNT         4237 a         5303 c         5993 g         5378 t
ORIGIN      
        1 ttcactccac tttcgtacat gcctcacggg aagttccgtc ttcggacact aatttgcctg
       61 atttatccag ttgatacaca tcatcttttc cgggtttgtc catctgcgcg accaatccgg
      121 tgtacatcat gctttccaag agtcgtgcat cctccgactt ttctaaccac gccagcgttt
      181 cggggctgtg ctgtcgtgaa acttcaccgt acttgatttg gctttgagtt aaggcattgt
      241 tcgcaaaacg ccatttacct tcaccgacaa cacggtatgc caagatattg ccgtcatcat
      301 cgattttgag tatagcatcg ccacggaagc tgccgtcctg aagatatttg acttttgtat
      361 cactgtgaat gttttcatca gtgccgatgc aatgccatgt accgacaatc ggagaagcca
      421 aggcggttgt tgcggataaa agcaccggca gaataaagcg tttcatctat ttcctttttt
      481 aaaggaggcc gtctgaaaac cccggtttca ggttttcaga cggccttgtt tattcaatca
      541 aatcagtctt tcaacttcgc caactgattt tgcacttttg ccattttgtc ttccaattcc
      601 gccaaatcgg ctttgtcttt ttccaccaga tgcgccgggg ctttttcggt gtagccgggt
      661 ttggagagtt tggcattgag tttgtccaag gctttttgca gcttctcggc ttctttgctc
      721 aaacgggcgg tttcggcggc tttgtcgatt tcgactttca gcatcaggcg cgcgccgttg
      781 cagacggcga cgggcgcgtc tccgctttcg ggcagggcgg cgacttgccg tgcttcggtc
      841 aggcgggtca tcatcggcag gtatttgagg tagtccgcca agtcgtccgc gctttcgaca
      901 aacagcgggg ctttcacgtt gggctggatg cccgtttcgc cgcgcaggtt gcggactgcg
      961 ccgatcaaat cctgcaacac ggtcatttgc ccgaatgccg tctgaacaat ctcgccgccg
     1021 tcggtttcgg ggaaacgggc gagcatgatg ctgtcggcgg ttttggcatc gcacatgggg
     1081 gcgacggttt gccacagttc ttcggtgatg aacgggataa tcgggtgcag caggcgcagg
     1141 gcggcttcga gtacgcgcaa taaggtatgg cgtgtggcgc gctggcggct ggcgcagccg
     1201 gtttggagct gcactttggc gagttccaga taccagtcgc aataatcgtt ccatacgaag
     1261 ctgtacaggg tttccgccgc caaatcaaag cggtaggttt cgtaggcttg cgtaacctgc
     1321 tcgatggtct gattcagacg acctatgatc cacatatcgg ggaaggaatg gccgcgcggt
     1381 tcggcagcgg ttgcgccgta gccgcagtct tggttttcgg tgttcatcaa gacgaagttg
     1441 gtggcgttcc agattttgtt gcagaagttg cggtagcctt cggcgcgttt gaagtcgaag
     1501 ttaaccgaac ggcccaagct ggcgtagctc gccatggtga agcgcaatgc gtccgcgccc
     1561 atgctgggga taccttcggg gaagagtttt ttcgtggctt cttccacttt cggcgcggtt
     1621 tcgggtttgc gcaggccggt cgtacgtttc atcagcagct tgtccaagcc gataccatcg
     1681 atcaaatcca cagggtcgat gacgttgcct tcggatttgg acattttttt gccttcgtgg
     1741 tcgcgcacga tgccgtggat gtacacggct ttaaacggta ctttgccggt gaagtgggtg
     1801 gtcatcataa tcatgcgcgc cacccagaag aagatgattt catatccggt aaccaagacg
     1861 ttggacggca ggaaggcttt gagctcgtcg gtttcagacg gccagccgag cgtggagaac
     1921 ggcacgagcg cggaggagaa ccatgtgtcc aatacgtctt cttcgcgggt caaacctgtt
     1981 ttgccggctt gtttttcggc ttcagcctga ttgcgggcga cgtaaacatt acctgcttcg
     2041 tcgtaccacg cggggatttg atgcccccac cacagttggc gcgaaataca ccagtcttgg
     2101 atgttgttca tccattggtt gtaggtattg acccagtttt cagggataaa gcgcactgcg
     2161 ccgctgtcga cggctttttt ggctttgtcg gcaaggctca agcctttgaa ttcgttgtca
     2221 ggctcgccgc cgttcggtgt ggcggacatg gcgacaaacc attggctggt cagcatcggc
     2281 tcaatcactg aacctgtgcg gtcgcctttc ggcgtcatca gcgtgtgcgg tttgatttcg
     2341 accaagaagc cttgctcctg caaatcggca accatttgtt tgcgcgcggc aaagcggtcc
     2401 aagcctgcgt atttttcagg cagggaaaag cctggttgcg cttcgccttt gaagttgaac
     2461 acttcggcgt ttgccagcac tttggcttct aagtcgaaca cgttaatcag gcgcgtgtcg
     2521 tggcgtttgc cgacttcgta atcgttgaaa tcgtgcgcag gcgtgatttt cacgcaaccg
     2581 gtaccgaagt ctttttcaac gtattcgtcg gcgataaccg gaatggtgcg accggtcagc
     2641 ggcaggatta attctttgcc gattaagtgg gtgtaacgtt catcttctgg attgacggca
     2701 acggccgcgt cgcccagcaa cgtttcagga cgggtggtcg ccacgataac ggcttcggtg
     2761 ggattgtcgg ccagcggata gcggatgtgc cacatagagc cttgttcttc catgctttcc
     2821 acttccaaat ccgataccgc cgtgccaagc acgggatccc agttcaccaa gcgtttgccg
     2881 cggtaaatca agccctgttc atacaggcgc acgaacactt cggtcacggt ttcggcgcgt
     2941 acgccgtcca tcgtgaaata ctcgcgcgtc cagtcggcgg agcagcccac gcggcgcatt
     3001 tgctgggtaa tcgtgccgcc ggaaacttct ttccattccc acactttttc caagaatttt
     3061 tcgcggccca agtcatgacg ggacacgttt tgcgcggcaa gctgacgctc gaccacgatt
     3121 tgcgtggcga tgcccgcgtg gtcggtaccg ggaatccagg cggtgttgca gcctttcatg
     3181 cggtagtagc gggtcaggcc gtccatgatg gtttggttga aggcatgacc catgtgcagc
     3241 gtgccggtta cgttgggcgg cggcagttgg atggaaaaag acggtttggt caaatccata
     3301 tcgggccgga aatagccttg gctttcccag ttttgatagt gtttggattc gatttcggcg
     3361 ggggagtatt tgtctaacat atgaagccct gagaaataag atgatttgat ggtttgaatt
     3421 atagcgcaaa tgccgtctga aacgctttcg gggtttcaga cggccttaaa tttaaatatt
     3481 gcctttacag tttttgatag cgcgacaggc tcaaatcgtc gctgcggatt tcggtgtctt
     3541 tgccgctcac gatatcggcg gtcaattttg ccgaacccgg cgacatggtc cagcccaaag
     3601 taccgtgccc ggtattcaga aacaggtttt caaagcgggt gcgcccgatt aacggcgtac
     3661 tgtcgggcgt catcggcctg aggccgctcc aggacaatgc ttggctcaaa tcgccgcctt
     3721 ccgggaacaa gtcgttgacg accaaagcca aggtttcgcg gcgtttttcg ggcagtttgg
     3781 tttcgtagcc ggacaattcc gccattccgc cgacgcggat tctgttgtca aaacgcgtga
     3841 tggcgacttt gtagctttca tctaaaacag tggacaccgg tgcgccgtcg gaatttgtga
     3901 ccggcagggt caaggaatag cctttgacgg gataaatggg cagattgaga tccaactgcg
     3961 ccaacacagt cctgctgaag cagccgagcg cgcagacaac ggcatctgtt tcaaaccgcc
     4021 ctgtttcggt ttcaacggct ttgatgcgca gcccgttgtg gtcgatgcgg ctgatggttt
     4081 ggttgaagta gaaccgtacg cccttctctt gacacaattt gtacaggttt tcggtaaaga
     4141 ggcggcagtc gccggtcgca tccgcaggca ggtgcagacc gccgacaatt ttggcggtaa
     4201 cgcgtgccag cgcaggctcg aattctgcgc attcttcggg cttcagacgg cggtacggca
     4261 cgccgtagcg ttccaaaacg gcaatgtctt gttttgccgc ttcgacttct tcggtttggc
     4321 ggaaaatctg caacgtccct tttttgcgtc cctcaaaatt catgtcggtt tgcgcttcaa
     4381 aacggcggaa catttcacgg ctgtattcgg aaatcctgac catgcgctct ttattgattt
     4441 gatagcgcgt tgccgtgcag ttttgcagca tccgccacag ccattcgatt tgatacaggc
     4501 cgccgtcagg gcggaacagt aaaggcggat ggcttttaaa cagccgtttc agtgctttgg
     4561 tcggaatacc gggtgcagcc caaggcgtgg tatagccgta agaaagctgg cctgcattgg
     4621 caaaactggt ttccatcgcc acaccctcgg tgcggtcgat gaccgttact tcatgtccgg
     4681 cctctgccag ataccacacg gaggatacgc cggcaacacc cgcacctaaa acaagcactt
     4741 tcatgtttcc ccctccggct ttttcaaaac aggcttaata tgccgtgccg tctgaatatt
     4801 cggattcaga cggtctcgga tattaatgcg gcaattcgcc gtttgtgatt ttttgtttga
     4861 agtcgcgtgt ttcattgacg atgactttcg ccatcaacaa aagcgcagtc aggttgggca
     4921 atgccatcaa gccgttgaat gtatccgaag ccagccatac caaatcaagg ctcaacacgg
     4981 tacccggcat gacggaagaa acataaccga cgcggtacaa agcggcaaac ttctcgccga
     5041 agacgtaaac cgcgcatttc tcgccgtaat aacaccagcc gagaatggtt gagtaggcaa
     5101 agaaaatcag gccgatggtc acaatccagc cgccgatgcc gggcagcatt ttttggaaag
     5161 tgacggttgt cagcgccgcg ccgctcaatt caggtttcac aaactcgccg cctgcgccga
     5221 gcagacccat gaccaacacg ataccggtaa tcgagcagac aacaatggtg tccaaaaaag
     5281 taccggtcat ggaaaccaaa gcctgacgaa cggggtggtc ggttttcgcg gctgcggcgg
     5341 caataggcgc agaacccata cccgcctcat tggagaacac gccgcgcgcc acgccgtagc
     5401 ggatgaccgt accgatagcg ccgcctgcta cggcctgcgc gctgaacgca tcggagaaaa
     5461 tcagcttgac ggcaggcatc agtgcatcgg aattaatgac gataatggaa agaccgccca
     5521 acacataaaa caccgccata gcaggcacga tgaaagaagc ggctttggcg atgcctttaa
     5581 taccacctaa aacgacaacg gcagtcagaa cggtcaacgt aatgccggta taggcaggtt
     5641 cgataccgaa gctggtttgc acagcctgtg caaccgagtt ggactgcacc gagctgccga
     5701 taccgaagga agcgaatgtg ccgaacagcg caaatgcaac ggccatccat ttccagtttt
     5761 tgcccaagcc tttttcgatg taatacatcg ggccgccgga catttcgcct ttggaattgt
     5821 tgacgcggta tttcaccgcc aacacgcctt cgccgtattt ggtggccatg ccgaaaatag
     5881 cggtcatcca catccaaaat accgcgcccg ggccgccggt taccactgca gtcgccacgc
     5941 cggcgatgtt acccgtaccg atggtggcgg acagcgcggt catcaacgcc gcaaaatggg
     6001 aaatatcgcc ttcgtggcct gcgccgcctt catacttctt tgacggcaca aacgcctgtt
     6061 tcagcgcata gcccaacata gtgaactgca agcctttcag cataacggtc agcacaatgc
     6121 cggtgccgac cagcagcatc aacataacag atccccaaac ccagctgctg acggtttcga
     6181 aaaaggcttt gagattgtct aaaaatactt gcatggcttt ctcctttgtc tgttttattt
     6241 ttaaaacacc acttttgtag tgtccggtaa tttcagcaca gaatattcaa taagacaata
     6301 tgttcttttg aaaaatactt ttggtttttt caccgaagac aggccggttc aagctgcgga
     6361 aattgtttgc aattatttaa aagcagaggc ggaggtcaca atgaaatgtc cgaatgggga
     6421 tgtggcgggc ggcagaaatc atcaatgctg ccgactgcca tacttctgaa atctatagtg
     6481 gtttaaattt aaaccggtac agcgttgcct cgccttgccg tacttcttgt actgtctgcg
     6541 gcttcgtcgc cttgtcctga tttaaattta aaccactata caaaatgatg catcgatcaa
     6601 acaacatacc gctttaaaaa aaaccgatgc cgtctgaaac gctttcgggg tttcagacgg
     6661 cattgaaagg gtgcggtcag cggatgatgc cgcgcgtcga ttgtgcgaag aagtcttgaa
     6721 atacggcaag ctcggcttgg gtttcggcgc ggcggagaat gtccgccttg gcttcttcaa
     6781 acggaatgcc gcgatggtag agggttttgt atacgtcttt gacggcggaa atctgttctg
     6841 cggtaaaacc gttgcggcgc ataccttcgc tgtttaggcc ggccggttcg gcgcggtagc
     6901 ccgatgccat aaagtagggc ggcacgtctt tgtgtacgcc tgcggcgaag gcggtcatgg
     6961 cgtagtcgcc gatgcggcag aattggaaga ccagcgtgta gccgcccaaa acgacgtagt
     7021 cgccgacggt aacgtgtccg gcaagcgagg cgttgttggc gaaaatggtg tggttgccga
     7081 ccacgcagtc gtgcgcgagg tggcagtacg ccataatcca gttgtcgtcg ccgatacggg
     7141 tttcgccgat gccggttacc gtgcctaaat taaaggtggt gaattcgcgg atggtgttgc
     7201 cgttgccgat aatcagcttg gtcggctcgt cgcggtattt tttgtcctgc ggaatttcgc
     7261 cgaggctggc aaattggaaa atgcggttgt tttcgccgat ggtggtgtgg ccgttgatga
     7321 cggcgtgcgg accgatttct gtattcgcac cgattcggac gttggggccg ataacggtgt
     7381 acgcgccgac tttgacgccg gagtcgagtt cggctttggg gtcgatgacg gcggtcgggt
     7441 ggatgagggt catgttttga atactccgct gcttgttaag acaaataata gaaaatcaca
     7501 gtttgtcgat ttcatcgcag accgcaatca cttgatcgct aaaatggcta aatactgaag
     7561 cagtatctac atccatgtaa ttaagcagtt caaaaataga ttggtaaata tttctagcgt
     7621 agccggtatc ttttattgca cctttaaaac aaatcggttc gtgatgtgcc aaacgattac
     7681 ggaaattgtt aatattggaa agttctcgaa aaatccattt ttggttatat tgcacacttg
     7741 gtgtagattt gggctttttt ggaaaaactt tcatcaatac ttttcctgca gcatcaaatt
     7801 gagcatcttt ccctcccgca aacagatacc gccaaaaacc gaatcctagt cctgcaacca
     7861 attggttgtg gtcaaacttt cctccgctat tattttgtat tttagataat gcaaccttga
     7921 ttaattcggc agattctttc gtaccatttc ttaaaaagca accttgatat ttcaatgcag
     7981 gattcgtttg tggttgtatg ctatcatata accggtttct gtctttaaac tcctgccgaa
     8041 agcaaatatc aattttgttt cttaaaacga tctcaaatat agaaacaaca tgaaaaagtt
     8101 ctcttgataa agataaattc aataaataca attcagctgc tttgtctttc ctaacggcat
     8161 ttttataccg ttccattctg aaatcagaaa aaatactaca gcattcctta taattcacgc
     8221 taacccttga ttctttattt ttaaattgac gtttcaaact atatttgata tagttagata
     8281 aaactaatcc ccaccgtgtc cctgggtttg aataaaccta aaccggtggg ttttcttttt
     8341 attaggtcgt ctgaaacttt cagacgacct ttttccgaac gctcaaacca cgcgtttggc
     8401 gcacataatc acggcttcga cggcgacttg tccgtccact ttggcaacgg cgttgaattt
     8461 gccgatgccg cgccggctgg tcaggagttc gacttcaaag acgagttggt cgccggggat
     8521 gacttggcgt ttgaaacggg cttcgtctat gccggcgaag aagaaaaatt cgttttcctt
     8581 gcgcccgcct tcgctcaaaa tcgccaacgt gccgcacgcc tgcgccatcg cttcgatgat
     8641 gagtacgccg ggcataacgg gcaggtcggg gaaatggcct tggaattggg gttcgtttat
     8701 ggttacgttt ttgatggcgg tcagggtttt catcggctcg aaggcggtaa tgcggtcgag
     8761 ctggagaaac gggtagcggt gggggatgag tttttggatg tctttggctt cgatggggag
     8821 ttgtacgtcc atgtctgtcg tattccttga ataaagtcgg tttggattta ttgcgtgtct
     8881 ttgctgtctg aaagctgctg ttccagcgtt ttgaggcgtt tgttcatttc gcttaagcgg
     8941 tggatgtaaa cggcgttgcg cgcccattct ttatgggtgg acatcgggaa gatgccggcg
     9001 atgtgtttgc cgctttcggt aatgctgtgg gtaacggacg tgccgccgcc gatggtggtt
     9061 ttgtcggcga tttcgatgtg tccgaccgtg ccgactccgc cgccgatgat gcagtagctg
     9121 cctatggtta cactgccgga gatgcccgtt ttggcggcga tgacggtgtg cgaaccgatt
     9181 ttgcagttgt gtccgatttg gacttggttg tcgattttgg tgccgttgcc gacaatggtg
     9241 tcgctcatcg cgccccggtc gatgttggtg ttcgagccga tttctacgtc gtcgcccagc
     9301 gttaccgcgc cggtttgcgg gattttgaac cacgaatcgc cggcgaaggc gagtccgaaa
     9361 ccgtccgcgc cgatgaccgc gccgctgtgg atttcgacgt gtctgccgag tgtgcagccg
     9421 taataaacga cggcgttggg atgcaggacg acttcgtcgc ccagtttgca atcgtgttgg
     9481 acgacggcgt ttgccaagat gcggcagcct tcgccgagca cggtgttcgc gccgatgtag
     9541 gcgttcgcgc cgatttcaca gcttgcggga acggtcgcgc ccggttcgac gacggcggtc
     9601 ggatggatgc cgccgcgcgc tttgacgacg ggtgaaaaca ggcgcgcgac tttggcgaaa
     9661 tagagatagg gatcgtcggc gacaatcagg ttgcgccctt caaatccgtc tgctgctttg
     9721 gcggaaacga tgatcgcgcc cgcgctgctg tcgtggactt cggctttgta tttcggattg
     9781 gcgaggaagc tgatgtgttc cgcctgcgcg tctgcgagcg ggcgcacggc ggtaacggaa
     9841 atgtcctcgc cgcgccattc gccgccgagc cgcgcggtga tttgggacag ggtgcaggtg
     9901 gccggaatca tggttttcct gtttggtatg ccgtctgaaa gggttagcgg gcgttcattt
     9961 ctttaatgac gctgtcggta acgtcgtatt gggtgttgac gtaaatcacg tcctgcaaaa
    10021 tgacatcgta accttcctgt ttggcgattt tgacgatgac gcggttggcg ttttgctgga
    10081 gggaggcaaa ctcttcgttg cggcggaggt tgtagtcttc ttcaaactgc gcctgttttt
    10141 tgcggaacgc ttcgaccagc ccgcgccatt tttcttcggc ttgcgccttt tttgcgtcct
    10201 taagtttgcc gccggcgagc tgcctttcca aatccaagcc ttcgcgttgc agtttttgca
    10261 attcgtcctg acgggcggaa aattcgccgt ccagcgtttt ttggatgttg cgcgcctgct
    10321 tggattcgag gtagatgcgc tcggtgttga taaagccgat tttttggaag gtgtcggcgt
    10381 gcgtgcctgt ggtgcagcat aaaccgatca gggtggcggc aaacgcgcgg gtcaaacggg
    10441 tcatggtaaa actccttcgg atgttgccgc gaaatgccgt ctgaagggct tcagacggca
    10501 tttgcgggat tagaacgtcg tgccgagctg gaattggaag cgttggattt cgtcttccgg
    10561 ttttttcttc agcgggtagg cgtagctgaa tttcatcggg cccaaaggcg agagccaggt
    10621 aaccgcgccg ccggcggaat agcgcaattc gttggtaaag gtggatttat gcgcgttttc
    10681 cgagtaaacc gatttgttgt taccgttttc ggcggcggta taggttctgc cgtcccacac
    10741 gctgcctgcg tcggcaaaca ggctcaggcg gacggtgcgt gcgtctttcg caccgggcat
    10801 cgggaagagc agctcggcgg agacgttggc ttttttgttg ccgccgtagc tgattttttc
    10861 gccgtattcg tcatacactt tcgggccgag cgtgccgctt tcgtagccgc gcaccgaacc
    10921 caggccgccg ccgtagaagt tttcaaagaa ggggatttct ttggttctgc cgtagccgcc
    10981 cgcaatgccg acttcgccgc cgagcatcag cgtgaaggtt ttgcttaagg ggaagaacca
    11041 ggtttggttg tgggtggcgg agtagtattg cagtttgctg ccgggcaggg cgatttcggc
    11101 atttacgccg gtcaggtagc cgcgcgtcgg ccataaggcg ctgtcggtct tgttgcgccc
    11161 ccagccgaca gtgcctttgt acagcaggcc tttgaagctg ccgtctgcgc cgtcggtttt
    11221 gccgtattgt ttgataaagt cggcatagcg tttgggtgct ttgttgtagg tgttgacggt
    11281 caggtgttcc gccgccagcc cgaaattgac gcggtcgtat tcggtaacgg ggatacccat
    11341 ccttacgccg ccgccggcgg tggtggtttt atattgtttg acgctggtcg atgctttgcg
    11401 cgggtcgaag gcttttccgt aaatatcgta gcccaggctg accccgtctg ccgtgaagta
    11461 cgggtcggta aacgacagcg agccgttgag cgtggttttg cttcgcgagg cgcgcagggc
    11521 ggccgacttg cccgtaccga acaggttgtc ctgcgatacg ccggcggaca tgaccaagcc
    11581 ggtatcctga acccagcccg cgctcaagtc gagcgagccg gtggagcgtt cggtcaggct
    11641 catgttcaaa tcgactttgt cgggcgtgcc ggcaagcggg acggcatcaa actgtacgtt
    11701 gtcgaagtag cccaaaagct cgacgcgctc tttggaacgt tgcagcttgg aggtgtcgta
    11761 aggcgcggat tccatttggc gcaattcgcg gcggacgact tcgtcgcggg ttttgttgtt
    11821 gccggtgatg tggatttcgt tgacgtagat ttttctgccc ggttcgatgt gcaggacgaa
    11881 atcgacggtt ttggttccgg cgttcggcag cggctgtacg ctgatttcgc tgtatgcgta
    11941 gcctgccgag cccatgcggt tctgaatctc acccaaaacg gcggtcatct gctggcgttc
    12001 gtaccatttg ccgggcttca tggtcagcag tttttccagt tcggccttgg ggacttcgtt
    12061 ggtgtcgcct tcaatcgaca ctttgcccca gcggaaacgt ccgccttcgt ggacggtgat
    12121 tttgatggtc tgcctggttt tgtcttcgtt ggtttggatg tcggtatcga ggatacggaa
    12181 atcgaagtag ccgttgttct ggtagaagtc ggttactttt tccatgtctt gggcgaattt
    12241 ctggcggtcg aaccggtcgc ttcgtgtcag ccatgtccaa atgccgcctt cggtcagcga
    12301 catctgccgc atcagtttgc ggtcggaata gacttggttg ccttcaaatt cgatgtcggt
    12361 gattttggcg gatttgccct cgtcaatcgt gatgtcgatg tcgacgcggt tgcgggcgag
    12421 tttggttact ttgggcgtga tttggatatt gagtttgccg cgcccgaggt attcttcttt
    12481 caggccggcg actgcctggt tgagtgtcgc ctgattaaag tattgcgact gcgccagccc
    12541 gaacgattcg aggtttttct tgatggcgtc gttttgcagc attttggcgc cggtgatgtt
    12601 gagcgagccg atggtggggc gttcgataac ggtcagcaga agctgcccgt ccgcagtttc
    12661 gactcgtacg tcgtcaaaga aaccggtggc gtacaggctt ttgatgatgg cactgccgtg
    12721 tgtgtcgttg taggtgtcgc cgactttgac gggcaggtag ttgaatacgg tgctcggctc
    12781 ggtacgctgc aagccttcga cacggatgtc ttggatggtg aagtcggcaa atgccaaagg
    12841 cgatatgccc aacatcatca gtgcggaggc aatctgtttc agtttcatcg taagttcctt
    12901 gtggtgcgga atgcggtttc agactgcatt ccgaaacgta aaatctaacc gatcagccgg
    12961 gtaacgtcgt tgaagaaggc ggccgccatc atcagcatca tcagggcgag cccgaagcgc
    13021 aaaccgatgt tttggacacg ttcgcccaaa ggtttgccgc gtatccattc gacagtataa
    13081 aacacgaggt gcccgccgtc caaaacgggg acgggcagca ggttcagcac gccgaggctg
    13141 atgctgacca acgctaaaaa ttccaaataa ctttgcaagc cgagttcggc ggactgtccg
    13201 gcaatgtcgg caatggtcag cggcccggaa atatggctga cagaggcgtt gccgctgatt
    13261 agtttgccga aaaatttgag ggttgtccac gagtgggaaa cggttttttc ccagcccatg
    13321 ccgaatgcgc ggacaacaga cggacggtag ctgcggcgga tttgcgcgtc ccacgccctg
    13381 tccggctgcg gacggaggcc gacgcgcccg atcagggtgt ggtcgggctg ttcgacagta
    13441 tcggggcgga tgtcggcggt atgggtttgt ccggcgcgtt cgtaggtcag ggtgattttt
    13501 ttgcccgggc tttggcgggt caggtttgcc cattcctgcc atgaggcgat gggtttgccg
    13561 tcggcggcag tcagcctgtc gcccggtttc aggcctgctt tttcggcggg gctgcctttt
    13621 tccacgccgc cggcaacggt tgtgatttta aagggcatca gtccgatgta gccttggttt
    13681 tttgcgattt taccggcttc cggcgtgcct gcggcatcga tggtgcggac ggtttgcgcg
    13741 cccgatgccg tctgaacgcc gacggcgact ttgccggctt cgaggttgag gacgatttcg
    13801 gtttgcgcgc tgctccagtc ttggacggaa acgccgttga cggattgtat tttgtcgccg
    13861 ctttggaagc cggtgcgggc ggcaatggtg tcgggttcga ctgtgccgac atagggccgc
    13921 agttcggtta cgccgaagga aaagctcagt ccgtacagca aaaccgccag tgcgaggttg
    13981 gtcagcggac cggcggcgac gatggcgatg cgcttggcgg ggtgttgttt gtcaaaagcg
    14041 tagggtaaat cggcttctga tacttcgcct tcgcgcgtat cgaccatttt gacgtagccg
    14101 cccaacggaa tcggggcgag gcaccattcg gtgtcgccgc gctttcgggt gaaaaacggt
    14161 ttgccgaagc cgacggaaaa acgcacaacc ttgacgccgc acaacctggc gacgatgtag
    14221 tgtccgaatt cgtgcaggct gaccaaaatc aggatggcga agataaaagc tagaagggtt
    14281 tgcaaatggt tttcctttga taacggtgtt cggacggtat cagcgcagtg tgccgataaa
    14341 tgcccgcgct tgtgcgcgtg tccgggcatc ttgcgccaac agtccttcta tatcgcccat
    14401 gccgtttgaa aagtcttgtg caagacagtg ggcgacggtt ttggcaatgt cggtaaactt
    14461 aatctgtccg tccaaaaagg cggcgacggc ggtttcgttg gcggcgttca atacgcaggg
    14521 cgcggctccg cctgcgttta tggtttcata ggcgaacttc aggcagggga agcggccgaa
    14581 gtcgggcttt tggaaggtca gcgcggacaa tgcgccgaaa tcgagtttgc cgacacccga
    14641 atcgatgcgc tcgggcaagc ccaaacaata ggcgatgggc gttcgcatat cgggattgcc
    14701 cagttgcgcc agcacggagc cgtcgcggta gcgcaccata ctgtgtatca cggattgggg
    14761 atggatgacg acttcgagtt tgtcgggcgg acagttgaac agccaatgcg cttcaatcag
    14821 ttccaagccc ttgtttgcca tagtggctga atcgacggag attttgcgcc ccatacgcca
    14881 attggggtgt ttgaccgcct gctcgggcgt aatgctgtcg aacgtgctta aatcggttgt
    14941 taaaaacggg ccgccggaag cggtcaggat aatcgaatcg atgccgtgtt cgttcagacg
    15001 gtctgtgtaa tcgcgcggca aaacttggaa aatggcgttg tgttcgctgt cgacgggcaa
    15061 cactgccgcg ccgtttgcgc gggcggtttc cataaacaac gcgccggaaa ccaccagcgt
    15121 ttctttgttt gccagataaa tggttttgcc tttttgcgcc gctgcgagcg cggaaggcag
    15181 ccccgccgcc ccgacgatgg cgcacatgac gccgctgact tcgtcggcag acgcaacgtc
    15241 aaccaatgcc tgcgcgccgt gtaaaacctg agtcgccgtg ccgtcgcgtt tcaacagggc
    15301 ttcaagccgg gcggcgtgtt cggcatcggc aacgacggca tattcggggc ggaacgtttg
    15361 acattgagcc gccagtttct cgacctgctt atgccctgcc agcgcgaata cgcggaattt
    15421 ttcgggatgg cgggagacaa cgtccagcgt gctttcgcct atgctgccgg tactgcctaa
    15481 tatggtcagg acttgtggtg tcataatgag gataacttta taccggatgc cgtctgaagc
    15541 gttttcagac ggcatagaat caatttaaaa ccgacattat cgccgcatag acgctgataa
    15601 cggcaatcag gctgtcggta cggtcgaaca cgccgccgtg tccgggcagc aggttgctgc
    15661 tgtctttgat gcctgccgcg cgcttgagcc agctttccaa caggtcgccg catacgctga
    15721 caacggtcaa caccaaaccg attaacacgg tatcgaacca gcctgtatcg aatgccagcc
    15781 agccggcact tcgtacggca gtcatgtaca ctgccacgca aaccgcaccg ccgattgcgc
    15841 cttcccagct cttgccgggg ctgattgccg gtgcgatttt gtgtttgccg agcgccttgc
    15901 cgctgaaata cgcgcaaacg tcggcaaccc agaccaaacc catcacggcg agcagcggca
    15961 gggcatcatc gggatgcggg cgcagggata cgagcgcgaa ccaaaacggc atgagcaaaa
    16021 gccagccgac ggcataaacc tgccaaccgc cgttgagcct ccatttgaat ctcaaccaca
    16081 aaggcataac ggcgagccaa aatgccaaaa caacatacca aaccaaatta ggcagcatcc
    16141 agccgcccgc ataggcaact acgccgaaaa ccaaggttgc ggcgaggtaa tggttggttt
    16201 cggttttgca caaaccggcc atacgggcat actcccacaa ggcggtcagg gcaatcagcc
    16261 cgcaaaatgc agcccacagc cattgcggcg cgtaaaacag catgcccagc atcagcggca
    16321 gcagccacat agcggttatt acccgttgtt tcagcatatt cagttccttt gctgtccgat
    16381 aggcagttgc tcggaagtgc gtccgaaccg ccgttcgcgt ttttggaacg aagcgacggc
    16441 ggcatctaag gcggtttcgt cgaaatcggg ccacaggatg tcggtgaaat acagttctgc
    16501 atacgccatc tgccagagca ggaaattgct gatgcgcgtt tctccgcccg tgcggatgaa
    16561 caaatccggt tccggcgcgt cgcccagcat caagtgtttg gccagcgtgt cttccgtaat
    16621 ctcggatacg ccttcggcaa tcagtttgtt tgccgcctgc aaaatatccc agcggccgcc
    16681 gtaatccgca gcaatgctca gggtcagacc ggtattattt gccgtcaagg cttccgcctc
    16741 ttcgatacct tgcagaatct gccgattaaa gcgttcgcgg ctgcccaata ttttcaggcg
    16801 catattgttt tcgtgcaggc ggcgtacctg tttttgcaaa gcctgtaaaa acagccccat
    16861 caggaacgaa acttcgtctt cggggcgacg ccagttttcg gttgaaaagg caaacacggt
    16921 cagatattgc acgcccagtt tggcgcaatg cttcaccata ttttccaacg cgtccaagcc
    16981 gcgtttgtgt cccattatac gcgggagaaa acgttttttc gcccaacggc cgttgccgtc
    17041 cataattacg gcgatgtgcc tcgggatggc ggtgtgttcc aaaatggtct gcgtgctgct
    17101 cttcatatct gcctttcgcg gttcggcgtt caaatgccgt ctgaacgccg cgccgtgaga
    17161 tttaaaccgc catcaggtct tcttctttgg cagccaaaac cttatcggct tcggcaatgt
    17221 acttgtcggt cagtttttga accgcctctt cgccgcgacg tgcctcgtct tcggaaattt
    17281 ctttgtcttt gaggagtttt ttgatgtggt cgttggcatc gcggcgcacg ttgcggatag
    17341 agacgcgtcc ttcttccgcc tcgccgcgca cgactttaat caggtctttg cggcgttcct
    17401 cggtcagcat gggcatcggc acgcggatca aatcgccgac agctgccggg ttcagtccca
    17461 agtttgaatc gcggatggct ttctcgactt tggccgccat attgccctca aacggtttca
    17521 cgccgatggt gcgcgcgtcc aaaagggtta cgttggcaac ctggctgacg gggaccatgc
    17581 tgccccagta ttcgacttcc acttggtcga gcaggccggt atgcgcgcgg ccggtacgca
    17641 ctttcgccag attttctttc agtacttcga ccgaacgctg catcttgcct tcggctgttt
    17701 tttgaatatc gttgatcata ttgttctttc ggtaagataa ggtaggcggg cagccgttcg
    17761 aacgcgctcc aagccgttca gacggcataa agaccgttaa ctgcgaatag taccgttatt
    17821 cgggcatgac gacaaggcag atgcctgtag gaagggtgtt tcacctttct agggtttttt
    17881 taattgccgc tgcaatttcg taggcaaggt tgacggggac ggcgttgcca atcattttgt
    17941 atgcgtcgtt gacattttga tagatgaatt tgaagttgtc gggaaagcct tggattcttg
    18001 caacttcgcg taccgtcatc ctccgatata gtgtttcttt gccggcagca aaacggtagt
    18061 cgtttgcccc gtgcttttcc atctttgggg cttgcgggtg cagttggcac tgcctgcctg
    18121 aagcttggac ggtaaaaccc tgttcatccc acgctttaac tcggttccgg ctcataaaaa
    18181 taggggaaaa actgccggta aaatattcgt tgttgttgac tgcgtcgggg ttggtcttgt
    18241 tttgcggggc ggaaggtacg gctgtgtcct gcaaatccca aataacgtct ttcaatgtaa
    18301 tcttgtcttt gtcttcgacc gtcgaacctt ttggaaaaga aaattttatt tccaagtctt
    18361 tacggaaacc gatgtagaag acccttttgc gttcctgtgc tacaccgtag tctttggcgt
    18421 tggccatagt caaggttacg tcgtatccgc atctgtcaaa cattttcagc aggttttgta
    18481 cggctccgtt gtggcggttt gccagcattc cgctgacgtt ttccgctaaa aagaattttg
    18541 gctgtttgct tttcaaaata cggatgtagt cgaaaaacaa ctgtccgcgc gcatcgtcga
    18601 tgccgcgcaa agctcccgct tcagaccaag actggcaggg tgggccgccg ataatcccgt
    18661 cgatttcttc agggaaatct tcttctttaa tcttgcgtat atcgccttct atcaaatggg
    18721 tctttggatg gtttgccttg aaggttgccc aaatggtttt atcgtattcg ttggcggcgg
    18781 ggatttcaaa tcccgctttt tcgaaaccca aatccaaacc gccgcaaccg ctaaacaaac
    18841 taatgatttt cataggatta taagtggtag gtgatgagtt ttgcatttct taatcgcgcc
    18901 gggttgttgg gatttttaat tttaatgtcg gaaatggcca gtttggaatc ctgtatggca
    18961 agcagcttgt ctgtattgtt aaaactgttc catttgtctt cgttgataat ggccatgaaa
    19021 ttgaaacttt tttccatatt ccgttggtaa atatagttga agacaaacca tggattttcg
    19081 attccccaca taccccgtac tctcagataa gttatgttga gcggatcaat gcggttgacc
    19141 ctgcccagct ctttggtttc tgcaaactgt atgccgccga tgttgccgat tccttcctta
    19201 atttgatttt ttatcttcaa atagcattcg gcatcggcgc agtagtcgat tccgtaaacc
    19261 attgccaagt gtttgaggtt tttctttttg tcaacgacgc cgacaattat agataatgtc
    19321 tttctcttcc catttttccg catctttgca ggcttttgta agcatgctgt catcaacaga
    19381 gagctttgat tttgggtggc tgctattcag cgccagtgcg gaatctttac tttcaatttt
    19441 cttaacttca atggcatccc cgtttcgcaa catggcatca ggagggttgg agttgttgcc
    19501 caaatatgag aatacttttg cgtgtcgggc tatgcgttgg gtttcattta ggttgaaact
    19561 gccggaaaac aagtctttta catattcttc caaagcatca cctgcctgat tggcacgatt
    19621 gttgctttga gaatgggagt tgaccccgac aacagggttg ttagccaaat tgatgatggc
    19681 atcgataatg ttcatggttt ttccttgggg ttttgcggca gattatagca aaaccgtcag
    19741 ggtgtttatc ggaccgatgc cgtctgaagc ggcaggcgtt tcagacggca tcgtgtccgg
    19801 ccgtcagccg tgttcccgtg tttcaagcag gctttggcgc aggtgttggc gttcgtgggc
    19861 atccagccat ttgcggcggg tgcgttgcag caggatgacg agggcggaaa tttcctgacg
    19921 catattggtg ctgagccaga ggaagccctg ccattggtag tggaggtgtt cggcgagggc
    19981 ttccagttcg gggttgatgg cggtgtcgat gcggatgcgg cgggcgtgtc tgccgttgat
    20041 gagggcggcg gtttgttgca ggtcggtttg gagcagtgtg aagtggcggt cgagcagccg
    20101 gatttcgctg ccgttgagtt tgggagattg cagcttggcg gcggtggtca ggagcagctc
    20161 ggtggtgttg acgattttgc ggtgggcgtg ctgcatggct tccatcatgg aggggctgat
    20221 gcggctttcg cccgatgtgg cggcgaggtg gctgcggctt ttgaccatgc gtgcgttgat
    20281 ttggcgcatt ttgaccatat tctgctccaa acgttcgcgc gtcatacgcc tgccgttgct
    20341 gatttcggca atcattttgc tgcagtcggc caggttgtcg gcaagcatga aacgccacat
    20401 cagtgtggat ttcagcggca gcagtttggc ggcggcaatg gcgatggcgg cgccgatgag
    20461 gacgttcatc gcgcgcatca ggccgctgtc gagccattcg ctgccgttgt cgccgatgag
    20521 catgcacatc gtcagccccg ccagcatagg gacgtagccg tttttgccga ccgccgccca
    20581 gccggccagt gcgcttgccg tgccgatggt caggtagaag aggaggttgc cgtggaaata
    20641 atgctggttc agccataaaa cgcccaaacc cgcgcccagc ccgatgaccg taccgagcat
    20701 acgttccacc gccttggagt aaatcgcgcc ttggaactgg agcatgccga ggacgacgaa
    20761 gacggtcatc cctatccatt cgccgtgttg gaggtggagt agccgggcga gtgcggtggc
    20821 gaacaggacg gctccgccga gccgcacggc atgtatgagg cggcggtggc ggtagcgttc
    20881 gtaggagttg agccagcggc cggaaaggcg t
//
LOCUS       DAWTQS010000033        20618 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.033, whole genome shotgun sequence.
ACCESSION   DAWTQS010000033 DAWTQS010000000
VERSION     DAWTQS010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20618
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.033"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(<1..161)
                     /locus_tag="WNA25_001200"
     CDS             complement(<1..161)
                     /locus_tag="WNA25_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823957.1"
                     /translation="MKRIFLPALPAILPLSAYADLPLTIEDIMTDKGKWKLETSLTYL
                     NSENSRSELA"
     gene            327..1250
                     /locus_tag="WNA25_001201"
     CDS             327..1250
                     /locus_tag="WNA25_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFA9823958.1"
                     /translation="MKKRNNPLPLLNGVKPSYLVLPHEKQFYGLPLLHFLCIRFPFLG
                     ADDWRRRLNSGFVVGSDGAALDEHSLFEPGKTMFYYRETGCESEPRIPFEEKILFVDE
                     HLIVVDKPHFLPVIPGGRFLRETLLTRLRLRPELQHLNVEDITPIHRLDKDTAGVMLL
                     SHNPATRGAYQTMFQNKTVWKTYEALAPTKTDLPYPLDVVSRLVRGEKFFTTQEAEGV
                     PNAHTTVELIENRGEFSLYRLTPHTGKKHQLRVHMMGLGMPLLNDALYPVPSEAGSED
                     YRKPLKLLAKKIAFADPLSGRERVFCSGFCL"
     gene            complement(1329..1970)
                     /locus_tag="WNA25_001202"
     CDS             complement(1329..1970)
                     /locus_tag="WNA25_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFA9823959.1"
                     /translation="MLKPETIHIPGPAGILETIHIPSEQVPARGVAVINHPNPLQGGT
                     NTNKVIQTAAKALSKLGFHCYLPNLRGVGGSEGTHDYGRGETQDCLAVIDYARAQHPE
                     APEFALSGFSFGGYVATFAAQVRIPDLLLLIGAAVCHYTDRPEPSAVPNVAKTLMIHG
                     AEDEVVEIEKALKWAEPQDLPVITIAGSTHFFHGKLIVLRDTILRFAPVCLNG"
     gene            complement(2025..2816)
                     /locus_tag="WNA25_001203"
     CDS             complement(2025..2816)
                     /locus_tag="WNA25_001203"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFA9823960.1"
                     /translation="MARHPYRRLRPAKSGFPEVGISEEGNIRSLHLGSDTVQSSMNLD
                     RPSELVLSYSRAMMGWLLFAERLPQHITQIGLGGGSFARWTDTYLPDTRQTAVDINPQ
                     VIAIARNLFELPFEGGKFEIIEADGAEYIKVFRHNTDIILVDGFDGEQIIDTLVEEPF
                     FRDCRNALSSDGIFVTNWWSGDKRYQRFIERLLSVFEGRVLKLPAESHGNVAVMAFQS
                     SPKEQNIDKLKKRADKLSNAYGLDFHRMLAGLKASNPNNGKHFHL"
     gene            2982..3057
                     /locus_tag="WNA25_001204"
     tRNA            2982..3057
                     /locus_tag="WNA25_001204"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3015..3017,aa:Thr,seq:tgt)
     gene            3199..3990
                     /gene="panB"
                     /locus_tag="WNA25_001205"
     CDS             3199..3990
                     /gene="panB"
                     /locus_tag="WNA25_001205"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFA9823961.1"
                     /translation="MITVNTLQKMKAAGEKIVMLTAYESSFAALMDDAGVDVLLVGDS
                     LGMAVQGRQSTLPVSLRDMCYHTECVARGAKNAMIVSDLPFGAYQQSKEQAFAAAAEL
                     MAAGAHMVKLEGGVWMAETTEFLQMRGIPVCAHIGLTPQSVFAFGGYKVQGRGGKAQA
                     LLNDAKAHDEAGAAVVLMECVPAELAKKVTETVSCPTIGIGAGADCDGQVLVMHDMLG
                     IFPGKTAKFVKNFMRGQSSIQAAVRAYVAEVKAKTFPAAEHIFAD"
     gene            4113..4949
                     /locus_tag="WNA25_001206"
     CDS             4113..4949
                     /locus_tag="WNA25_001206"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFA9823962.1"
                     /translation="MQIIHTIRELRTWRENIGKVAFVPTMGNLHEGHLALVREARKRA
                     DNVVVSIFVNRLQFGQGEDFDKYPRTLQQDADKLAAEGVAVVFAPDEKELYPNVEQRY
                     NVEPPHLQNELCGKFRPGHFRGVATVVSKLFNIVLPDVACFGKKDYQQLAVIKGLTED
                     LNFDIEIVPVDTGRAADGLALSSRNRYLSVGERAEAPRLYRELQAVAESLKQGGLDYA
                     GLERQAADHLTAAGWLVDYVEIRRADTLEMARAGDKKLVVLAAARLGTTRLIDNVEVG
                     LP"
     gene            5276..7114
                     /locus_tag="WNA25_001207"
     CDS             5276..7114
                     /locus_tag="WNA25_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823963.1"
                     /translation="MLPARFTILSVLAAALLAGQAYAAGAADVELPKEVGKVLRKHRR
                     YSEEEIKNERARLAAVGERVNRVFTLLGGETALQKGQAGTALATYMLMLERTKSPEVA
                     ERALEMAVSLNAFEQAEMIYQKWRQIEPIPGEAQKRAGWLRNVLREGGNQHLDGLEEV
                     LAQSDDVQKRRIFLLLVQAAVQQGGVAQKASKAVRRAALKYEHLPEAAVADAVFGVQG
                     REKEKAIEALQRLAKLDTEILPPTLMTLRLTARKYPEILDGFFEQTDTQNLSAVWQEM
                     EIMNLVSLRKPDDAYARLNVLLEHNPNANLYIQAAILAANRKEGASVIDGYAEKAYGR
                     GTGEQRGRAAMTAAMIYADRRDYAKVRQWLKKVSAPEYLFDKGVLAAAAAAELDGGRA
                     ALRQIGRVRKLPEQQGRYFTADNLSKIQMLALSKLPDKREALIGLNNIIAKLSAAGST
                     EPLAEALAQRSIIYEQFGKRGKMIADLETALKLTPDNAQIMNNLGYSLLSDSKRLDEG
                     FALLQTAYQINPDDTAVNDSIGWAYYLKGDAESALPYLRYSFENDPEPEVAAHLGEVL
                     WALGERDQAVDVWTQAAHLTGDKKIWRETLKRYGIALPEPSRKPRK"
     gene            7254..7835
                     /locus_tag="WNA25_001208"
     CDS             7254..7835
                     /locus_tag="WNA25_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HFA9823964.1"
                     /translation="MKHTVSASVILLLTACAQLPQNNENLWQPSEHISSFAAEGRLAV
                     KAEGKGSYANFDWTYQPPVETININTPLGSTLGQLCQDRDGALAVDGKGNVYQAESAE
                     ELGRQLVGFKLPIQYLHIWAEGRRVAGAPYRIRSDGILEQYGWTIGRTADSGGQVRTL
                     QLNNGNLNIRLVFTEIGMPSETETPERCAARTR"
     gene            7845..8690
                     /gene="ispE"
                     /locus_tag="WNA25_001209"
     CDS             7845..8690
                     /gene="ispE"
                     /locus_tag="WNA25_001209"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFA9823965.1"
                     /translation="MNIADGRQAFPAPAKLNLDLRITGRREDGYHNIESIFCLIDLQD
                     TVYLKPRDDGKIILHNPVGGIPQEADLSYRAASLLQKYARNLAGVEIWLDKKIPTGAG
                     LGGGSSDAATVLLVLNRWWQCGLTQWQLIDLGAALGADVPFFIFGKNAFASGIGEKLI
                     GMDIPKQWYVIVKPPVHVSTAKIFTYEGLTRDSASSIMPTFQNLQPFRNDMQAVVFKE
                     YPEVWKAYSELSKYGSAMMTGSGACIFAAFQARNSAYNIYRQVSGLYEAYLAEGLSKH
                     PLLSV"
     gene            8698..8773
                     /locus_tag="WNA25_001210"
     tRNA            8698..8773
                     /locus_tag="WNA25_001210"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8731..8733,aa:Gln,seq:ttg)
     gene            8786..8861
                     /locus_tag="WNA25_001211"
     tRNA            8786..8861
                     /locus_tag="WNA25_001211"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8819..8821,aa:Gln,seq:ttg)
     gene            9029..10012
                     /locus_tag="WNA25_001212"
     CDS             9029..10012
                     /locus_tag="WNA25_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HFA9823966.1"
                     /translation="MAAYDSLMVFTGNANPELAQRVVRHLDISLGNASVSKFSDGEVA
                     VELLENVRGRDVFILQPTCAPTNDNLMEILTMADALKRASAGRITTAIPYFGYARQDR
                     RPRSVRVPISAKLVANMLYSAGIDRVLTVDLHADQIQGFFDIPVDNIYATPILLNDIK
                     QQRIENLTVVSPDIGGVVRARAVAKSLNADLAIIDKRRPKANVAEVMNIIGDIQGKTC
                     LIVDDMIDTANTLCKAAVALKERGAERVLAYASHAVFSGEAVSRIASSEIDQVVVTDT
                     IPLSEAAKNCDRIRQVTIAGLLAETVRRISNEESVSYLFNEEVMTGSMLLP"
     gene            10079..10651
                     /locus_tag="WNA25_001213"
     CDS             10079..10651
                     /locus_tag="WNA25_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFA9823967.1"
                     /translation="MTYEIQASVREAQGTGASRRLRREGQIPGILYGEGQEPVAIAVD
                     HKTVFYALEKESFHTALIKLSLNGETKDVIVRDFQMHPFRREVQHIDFQAVKADQLVR
                     IRVPLHIVNAENSQAVKLQGGRVSLLNTAVEVVALPANIPAFLDLDCAEVVAGDILHL
                     SDIKLPEGVESVSLKRNENLAVATVTGKKR"
     gene            complement(10777..11946)
                     /locus_tag="WNA25_001214"
     CDS             complement(10777..11946)
                     /locus_tag="WNA25_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HFA9823968.1"
                     /translation="MTAHKILPVLLPIILGVSHATAASPAPNRPTVHAAPTLQTPETL
                     TAAHIVIDLQSRQTLSAKNTNTPVEPAALTQLMTAYLVFKNMKSGNIQSEENLKIPES
                     AWASEGSRMFVRPGDTVSTDKLLKGMIALCANDAALTLADRLGNGSIENFVQQMNKEA
                     RRLGMKNTVFKNPTGLGREGQVSTAKDLSLLSEALMRDFPEYYPLFSIKSFKFENIEQ
                     NNRNILLYRDNNVNGLKAGHTESGGYNLAVSYSGNGRHILVITLGSESAETRASDNSK
                     LLNRALQAFDTPKIYPKGKTVAQIQISGGSKKTVRAGFLKEAYITLPHKEAKMAEQIL
                     ETIQPIPAPVKKGQILGKIKIRQNGHTIAEKEIVALENVEKRSRWQRLWTRLTGQ"
     gene            12095..13621
                     /gene="ilvA"
                     /locus_tag="WNA25_001215"
     CDS             12095..13621
                     /gene="ilvA"
                     /locus_tag="WNA25_001215"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFA9823969.1"
                     /translation="MNTPLPYSDYLIRILTASVYDVAVETPLEPARSLSVRLKNNILL
                     KREDLQPVFSFKIRGAYNKMSKLPKDALACGVIAASAGNHAQGVALSAQRLGCRAVIV
                     MPETTPKIKVDAVKSRGGEVVLRGVSYNDAYDYAMELAEQEGLTYIAPFDDPDVIAGQ
                     GTVGMEIVSQHPDPIRAVFVPIGGGGLAAGVAAFIKQVRPEIKVIGVQTNDSCCMKQS
                     VEAGEIVHLKDVGLFSDGTAVKVVGNGTFRLCKELLDEIITVDTDAVCGAVKDIFDDT
                     RSITEPAGALALAGLKAYIAREGAENQTLIAVTSGANMNFHRLRHVSERSELGEGNEG
                     IFAVTIPEERGSFLKFVNILGNRNITEFNYRYGDDEKAHIFVGLQAAGPQDLAVIGSR
                     LDEAGLPNVDLTNNEIAKIHIRYMVGGRTDKVGHERLVSFEFPERPGALARFLNHMQG
                     GWNITLFHYRNHGADYGRILVGIDVPPHDAAAFDGFLESLGYSYHEETQNAAYKLFLA
                     "
     gene            complement(13677..14753)
                     /locus_tag="WNA25_001216"
     CDS             complement(13677..14753)
                     /locus_tag="WNA25_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HFA9823970.1"
                     /translation="MSITIQNLNKKRFGNFHALKNINLNVPAGKLVSLLGPSGCGKTT
                     LLRIIAGLENADGGQILFDGQDVTAKHVRERKVGFVFQHYALFRHMNVFDNVAFGLTV
                     LPKPERPSKGQIRAKVEELLKLVQLSHLAKSYPHQLSGGQRQRIALARALAVEPKLLL
                     LDEPFGALDAKVRKELRTWLRDIHHNLGVTSILVTHDQEEALEVSDEIVVMNHGKIEQ
                     TGSAEAIYRKPENAFVTEFLGEADAFEGRIEKGIWHYNGFAWKLDAQYKWQEQTATGY
                     IRPHEWQIAAEHETPMIRAEIEKIHAVGALTHVLVKHGKQDVHITLAGSDAARYPIAE
                     GKKLNLIPKQVYVFSQNELIEYSI"
     gene            complement(14791..14898)
                     /locus_tag="WNA25_001217"
                     /pseudo
     CDS             complement(14791..14898)
                     /locus_tag="WNA25_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(14894..15715)
                     /gene="cysW"
                     /locus_tag="WNA25_001218"
     CDS             complement(14894..15715)
                     /gene="cysW"
                     /locus_tag="WNA25_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HFA9823971.1"
                     /translation="MKPYSANPNLTEPRRLRMLLIAAALGFLLLMLVVPLVAVFYEAL
                     KGGWDLYLKSLSDPEAWSAVKLTLITALIAVPVNAVLGVAMAWLLTRFDFLGKQLLTT
                     LLDLPFSVSPVVAGLMFVLLFGAHTALGSRLEAQGIQIIFAIPGIVLTALFVTFPFAA
                     REIIPLMQTQGDSEEQAALVLGASGWQMFWRVTLPNIKWTLLYGIILTNARAMGEFGA
                     VSVVSGHIRGETNTIPLLVEIFYNEYNFTGAFSLSGVLALLALATLAVQNIITKL"
     gene            complement(15904..16740)
                     /gene="cysT"
                     /locus_tag="WNA25_001219"
     CDS             complement(15904..16740)
                     /gene="cysT"
                     /locus_tag="WNA25_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HFA9823972.1"
                     /translation="MLPLKTPGVLPGFKLSLGLTVLCLSLLVVLPFAMMAAKAAEIGW
                     DGFRNTIAEPNVLAAVRLSLRMSFYAMLTNVVFGTLVAWVLVRYEFPGKGLANALVDL
                     PFALPTAVTGIALAALYAPNGWIGRFFEPLGIKIAFTPVGIWIALVVVSLPFIVRAVQ
                     PVLEELSGEYEEAAATLGANRFTTFRRVLLPEITPALLTGAGMMFARATGEYGSVIFI
                     AGSIPMISEILPLIITGKLEQFDAQGASAVALFMLLVSFVILFALNVMQWALGRRSGA
                     KG"
     gene            16921..17253
                     /locus_tag="WNA25_001220"
     CDS             16921..17253
                     /locus_tag="WNA25_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspG"
                     /protein_id="HFA9823973.1"
                     /translation="MQILSFQADIAERMLEGTEGESVNENAQFVRTDNGYWIAWHEGV
                     AALLAPDTPPGIPCFWVEGAESLEELCAMVERGEFDEVEEFDGDDDAWLEAAKDCGHH
                     GDACACGH"
     gene            17584..18093
                     /locus_tag="WNA25_001221"
     CDS             17584..18093
                     /locus_tag="WNA25_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003662333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HFA9823974.1"
                     /translation="MTMILSILSLFFIIRLLFLAVSIKHEKALIAKGAKQYGKTNSTV
                     LAAVHTLYYLACFVWVWLSDTAFNGISLIGTLTVMASFVILSLIIKQLGEIWTVKIYI
                     LPNHQINRSWLFKTFRHPNYFLNIIPELIGIALLCQAWYVLLIGLPIYLLVLFKRIRQ
                     EEQAMATLF"
     gene            complement(18325..18906)
                     /locus_tag="WNA25_001222"
     CDS             complement(18325..18906)
                     /locus_tag="WNA25_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFA9823975.1"
                     /translation="MEHKLPQLPYEPDALSPHLSKETLEFHYGKHHQTYITNLNNQIK
                     GTEFENLPLEEIVKKSSGGVFNNAAQTWNHTFYWLGFTPKGQGKPAGELAAAIDAKWG
                     SFEKFQEAFNACAAGTFGSGWAWLVKTPVGGLDLISTSNAATPLTTENTPLLTCDVWE
                     HAYYIDYRNSRPNYLEGFWEIVNWDEVAKRFAA"
     gene            19070..20476
                     /gene="dnaB"
                     /locus_tag="WNA25_001223"
     CDS             19070..20476
                     /gene="dnaB"
                     /locus_tag="WNA25_001223"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFA9823976.1"
                     /translation="MNDYAAMPPEDREVGALSLPPHSMEAEQSVLGGLMLENPAWDRI
                     ADVVSGEDFYRHEHRLIFRSIAKLINEGRPADVITVQEDLQRNEELEAAGGFEYLITL
                     AQNTPSAANIRRYAEIVRERSIMRQLAEVGTEIARSAYNPQGRDAGRLLDEAENKVFQ
                     IAESTAKSKQGFLEMPDLLKEVVQRIDMLYSRDNPDEVTGVPTGFIDLDKKTSGLQPG
                     DLIIVAGRPSMGKTAFSINIAEYVAIEKHLPVAVFSMEMGGAQLVMRMLGSVGRLDQS
                     VLKTGRLEDEHWGRLNEAVVKLSDAPMFIDETPNLTALELRARARRLARRFDGRLGLI
                     VIDYLQLMAASGRSDNRASELGEISRSLKALAKELQVPIIALSQLSRTVEQRTDKRPM
                     MSDLRESGAIEQDADLIMFMYRDEYYNQESQMKGYAECIIGKHRNGPVGKIFLTWTGQ
                     FTKFDNAAYIPEEAKIED"
BASE COUNT         4813 a         4898 c         5855 g         5052 t
ORIGIN      
        1 gcaagttcgg aacggctgtt ttcgctgttc aggtaggtaa gggaagtttc cagtttccat
       61 ttgcccttgt cggtcattat gtcttcaatc gtcaagggca ggtcggcata agcggataaa
      121 ggcaggatgg cgggcaaggc gggcaaaaag atacgtttca tatttcctct tattgatatt
      181 aattcttatt taataatagt aatgaatatc cgataattct gtttaaacaa atttttaaat
      241 caaaaaaaaa cgatatattt gtttttatca attatgtttt gatatgccgt ctgaaaagtt
      301 tgtgaaaaac ggttacaatc cgccgcatga aaaaacgcaa taatcctctt ccgctgttga
      361 acggtgtcaa acccagttat ttggtgctgc cgcatgaaaa gcagttttac gggctgccgc
      421 tgctgcattt tctgtgcatc cgctttcctt ttttgggagc ggacgattgg cggaggcggt
      481 tgaacagcgg ttttgtggtc ggttcggatg gtgcggcgtt ggacgaacat tctttgttcg
      541 agcccggtaa gacgatgttt tattaccgtg aaaccggttg tgagagcgag ccgcgtattc
      601 cgtttgaaga aaagattttg tttgtcgatg aacatttgat tgtggtggac aagccgcatt
      661 ttctgcccgt catccccggc gggcgttttt tgcgggaaac gctgctcacg cgtctgcgcc
      721 tgcgtcccga attgcagcat ttgaatgttg aagacattac gccgattcac cgcttggaca
      781 aggatacggc gggtgtgatg ctgctgtcgc acaatcctgc cacacgcggg gcttatcaga
      841 cgatgtttca aaacaaaacg gtatggaaaa cgtatgaggc gcttgcgccg acaaagacgg
      901 atttgccgta tccgctcgat gtggtttcgc gtttggtaag gggtgagaaa ttttttacga
      961 cgcaagaggc cgaaggcgtg ccgaatgcgc acacgacggt cgaattgatt gaaaacaggg
     1021 gggaattcag cctttaccgc cttacgccgc ataccggcaa gaaacaccag ttacgcgtgc
     1081 atatgatggg tttgggtatg ccgctgttga atgacgcgct ctatcccgtg ccgtctgaag
     1141 cgggtagcga ggattatcgg aaacctttga agcttttggc aaaaaagatt gcgtttgcag
     1201 atcctttgtc gggtcgggaa agggtgtttt gcagcgggtt ttgcctgtaa tgagagggaa
     1261 cgcaaacccg tatcggcaga tgccgccgga tacgggtttg atattatcga tgctgtctga
     1321 aaggggcttt atccgttcag gcatacgggt gcgaagcgga ggatggtgtc gcgcaggacg
     1381 atgagtttgc cgtgaaagaa gtgcgtcgaa ccggcgatgg taatgacggg caaatcttgc
     1441 ggttctgccc atttcagggc tttttcgatt tcgacgacct catcttccgc gccgtgtatc
     1501 atcagcgttt tggcaacgtt gggaacggcg gacggctcgg ggcggtcggt atagtggcag
     1561 actgccgcac cgatgagcag caataaatcg ggtatgcgca cttgtgcggc aaatgtggcg
     1621 acatagccgc cgaaggaaaa gccggataag gcaaattccg gggcttcggg atgttgggcg
     1681 cgggcatagt cgatgacggc gaggcagtct tgcgtttcgc cgcgtccgta atcatgtgtg
     1741 ccttcgctgc cgcctacgcc gcgaaggttg ggaaggtagc agtggaagcc gagcttgctc
     1801 aatgctttgg cggcggtttg gatgactttg ttggtgttcg tcccgccttg gagggggttg
     1861 gggtggttga tgacggcaac accgcgtgcc ggtacttgtt cggacgggat atggatggtt
     1921 tctaaaatgc cggcagggcc gggtatgtgt atggtttcgg gtttcagcat aatggtttcc
     1981 tcttgatttt ccgcatattc cgatgcagcc gcgaagatgg cggcttaaag gtggaaatgc
     2041 ttgccgttgt tggggttgga cgctttcagg ccggcgagca tgcggtggaa gtccaatccg
     2101 tatgcgttgc tcagtttgtc ggcacgtttt ttgagtttgt ctatgttttg ctctttgggg
     2161 ctgctttgga aggccattac cgcgacattg ccgtggcttt cggcaggaag tttcaatacg
     2221 cgcccttcaa aaacgctcaa caaccgttcg atgaagcgtt gatagcgttt gtcgccgctc
     2281 caccagttgg taacgaatat gccgtctgaa gagagtgcgt tgcggcagtc tctgaaaaac
     2341 ggttcttcaa ccagcgtatc gatgatttgt tcgccgtcga atccgtccac caagatgata
     2401 tcggtgttgt ggcggaagac tttgatgtat tccgcaccgt ctgcttcgat aatttcaaat
     2461 tttccgccct cgaaaggcaa ctcaaacagg ttgcgggcaa tggcgatgac ctgaggattg
     2521 atgtccacgg cggtttggcg cgtgtcgggc aggtaggtgt ctgtccagcg tgcaaacgaa
     2581 ccgccgccca agccgatttg ggtgatatgt tgcggcaggc gttccgcaaa cagcagccag
     2641 cccatcatcg cgcggctgta agagagcacc agctcggacg ggcggtcgag gttcatcgag
     2701 ctttgaacgg tgtcgctgcc caagtgaagc gaacggatat tgccttcttc ggaaataccg
     2761 acttcgggaa agccggattt tgcaggacgc aggcggcggt agggatgtct tgccatcggt
     2821 gtgaacggtc ggtttgaaag gcggatattt tatcagaatg ccgatgcttg ttctgtttca
     2881 aattaatttt gttttaaata agatacttag tgtattttta tgagggggtc gaacaaattt
     2941 cttgccaaag tgtgaagttg cctgcataat tcgccttctt tgccggtata gctcagttgg
     3001 tagagcacct gacttgtaat cagggggtcc cgagttcgac tcttggtgcc ggcaccagat
     3061 ttgacagccc gattgtgtaa aacagtcggg ctgttttgca tttgcggcac ggtcggtggc
     3121 agggctgcgg gcatttcact ataatagccc gtttcaaact gaaaaggccg tctgaaaagg
     3181 gcggggtaac aatatctgat gattactgtg aacacactgc aaaaaatgaa ggcggccggc
     3241 gagaaaatcg ttatgctgac cgcttacgaa tccagttttg ccgcgctgat ggacgatgcc
     3301 ggcgtggatg tgctgttggt cggggattct ttgggaatgg cggttcaggg gcgacagtcg
     3361 acgctgccgg tcagcctgcg cgatatgtgc tatcacaccg aatgtgtagc acgcggtgca
     3421 aaaaatgcga tgattgtcag cgatttgccg tttggtgcat atcagcagag taaggagcag
     3481 gcgtttgccg ccgccgccga actgatggct gccggcgcgc atatggtcaa actcgaaggc
     3541 ggcgtgtgga tggcggaaac gactgaattt ctgcaaatgc gcggaattcc ggtctgtgcc
     3601 cacatcggtc tgaccccgca atccgtgttt gccttcggcg gatataaagt tcaggggcgc
     3661 ggcggcaagg cgcaggcgtt gcttaacgat gccaaggcgc atgatgaagc cggagctgcc
     3721 gtcgtactga tggaatgcgt gccggcggaa ctggcaaaaa aggtaactga aactgtttcc
     3781 tgtccgacca tcggcatcgg ggcgggcgcg gattgcgacg gtcaggtttt ggtgatgcac
     3841 gatatgctcg gtattttccc gggcaaaacg gcgaaattcg tcaaaaactt tatgcgcggg
     3901 caaagcagca tccaggctgc ggttcgggcg tatgttgccg aagtcaaagc caaaaccttc
     3961 cctgctgcgg aacatatttt tgcagattga acaaggtctg ctgccgatgc cgtctgaaag
     4021 ccgtttcaga cggcattttg ttttgccgtg cgcggcgcgt ataatcggcg cgttttgtcg
     4081 ggcaggaagc ccgaaggata aggattaccg taatgcaaat catacatacc attcgagaac
     4141 tgcgcacgtg gcgtgaaaat ataggaaagg tggcatttgt gccgaccatg ggcaatctgc
     4201 atgaagggca tcttgccctt gttcgcgaag ccagaaaacg cgccgacaac gttgttgtca
     4261 gcatattcgt caaccgcttg caattcggtc agggcgagga tttcgacaaa tatccgcgta
     4321 ctttgcaaca ggatgcggac aaacttgccg ccgaaggcgt tgccgttgtt ttcgcgcccg
     4381 atgagaaaga actctatccg aacgtggaac agcgttacaa cgtcgaaccg ccccatttgc
     4441 aaaacgaatt gtgcggcaaa ttccgccccg gccattttcg cggcgtggcg acggtggtgt
     4501 ccaagctgtt caatatcgtt ttgccggatg ttgcctgttt cggcaaaaaa gattaccagc
     4561 agcttgccgt gattaaaggt ttgaccgagg atttgaattt cgatatcgaa attgtccccg
     4621 tcgatacggg gcgggcggcg gacggtttgg cactctccag ccgcaaccgg tacttgagcg
     4681 ttggggaacg cgccgaagca ccgcgcctgt accgcgagct gcaggctgtt gccgaatcgc
     4741 tgaagcaggg cggtttggat tatgcgggtt tggagcggca ggctgccgac catttgaccg
     4801 ctgcgggctg gctggtcgat tatgtggaaa tccgccgcgc cgatacgctc gaaatggcgc
     4861 gggcgggaga taagaaactg gtggtcttgg ccgccgcccg tctggggacg acgcgtctga
     4921 ttgacaatgt ggaagtcggc ctgccttagc ccgccggagc cgggaatgcc gcctgaagcg
     4981 gatttgcgtt tcagacggca ttttgccgtc ggctgtgctt cggaataatg tcggaaaata
     5041 gacggaatga aataaatttc tttttgttca acaaattaaa ctgtcaaacc aaataataag
     5101 gcaaggattg tccggcatcg catacaggca aaccgacgca atctcaaaac acagccctgc
     5161 cttcgggcgg gttttgcaaa tctacaaacc gtttggcttg tgataaagtg acgccttgtc
     5221 tagatactca atatgccgtc tgaaggttcg gacggatgtc ggataaagga tgattatgtt
     5281 acccgcccgt ttcactattt tatctgtcct cgcagcagcc ctgcttgccg gacaggcgta
     5341 tgctgccggc gcggcggatg tggagctgcc gaaggaagtc ggaaaggttt taaggaaaca
     5401 tcggcgttac agcgaggaag aaatcaaaaa cgaacgcgca cggcttgcgg cagtgggcga
     5461 acgggtcaac agggtgttta cgctgttggg cggtgaaacg gctttgcaga aagggcaggc
     5521 gggaacggct ctggcaacct atatgctgat gttggaacgc acaaaatccc ccgaagtcgc
     5581 cgaacgcgcc ttggaaatgg ccgtgtcgct gaacgcgttt gaacaggcgg aaatgattta
     5641 tcagaaatgg cggcagatcg agcctatacc gggtgaggcg caaaaacggg cggggtggct
     5701 gcggaacgta ttgagggaag ggggaaatca gcatctggac gggttggaag aggtgctggc
     5761 gcaatcggac gatgtgcaaa aacgcaggat atttttgctg ctggtgcaag ccgccgtgca
     5821 gcagggtggg gtggctcaaa aagcatcgaa agcggttcgc cgtgcggcgt tgaaatatga
     5881 acatctgccc gaagcggcgg ttgccgatgc ggtgttcggc gtacagggac gcgaaaagga
     5941 aaaggcaatc gaagctttgc agcgtttggc gaagctcgat acggaaatat tgccccccac
     6001 tttaatgacg ttgcgtctga ctgcacgcaa atatcccgaa atactcgacg gctttttcga
     6061 gcagacagac acccaaaacc tttcggccgt ctggcaggaa atggaaatta tgaatctggt
     6121 ttccctgcgt aagccggatg atgcctatgc gcgtttgaac gtgctgttgg aacacaaccc
     6181 gaatgcaaac ctgtatattc aggcggcgat attggcggca aaccgaaaag aaggtgcgtc
     6241 cgttatcgac ggctacgccg aaaaggcata cggcaggggg acgggggaac agcggggcag
     6301 ggcggcaatg acggcggcga tgatatatgc cgaccgcagg gattacgcca aagtcaggca
     6361 gtggttgaaa aaagtgtccg cgccggaata cctgttcgac aaaggcgtgc tggcggctgc
     6421 ggcggctgcc gaattggacg gaggccgggc ggctttgcgg cagatcggca gggtgcggaa
     6481 acttcccgaa cagcaggggc ggtattttac ggcagacaat ttgtccaaaa tacagatgct
     6541 cgccctgtcg aagctgcccg acaaacggga agccctgatc gggctgaaca acatcatcgc
     6601 caaactttcg gcggcgggaa gcacggaacc tttggcggaa gcattggcac agcgttccat
     6661 tatttacgaa cagttcggca aacggggaaa aatgattgcc gaccttgaaa ccgcgctcaa
     6721 acttacgccc gataatgcac aaattatgaa taatctgggc tacagcctgc tttccgattc
     6781 caaacgtttg gacgagggtt tcgccctgct tcagacggca taccaaatca acccggacga
     6841 taccgccgtt aacgacagca taggctgggc gtattacctg aaaggcgacg cggaaagcgc
     6901 gctgccgtat ctgcggtatt cgtttgaaaa cgaccccgag cccgaagttg ccgcccattt
     6961 gggcgaagtg ttgtgggcat tgggcgaacg cgatcaggcg gttgacgtat ggacgcaggc
     7021 ggcacacctt acgggagaca agaaaatatg gcgggagacg ctcaaacgct acggaatcgc
     7081 cttgcccgag ccttcccgaa aaccccggaa ataatgcagg cccatccttt cagacggcat
     7141 aaggtttgcc gggaagccgg ggcattcggg caaacggcac gcagttcgca cgcgttttgc
     7201 acggcacgcc gcacccgtcg gccggcagga tgacacccgt taaggaaact ctgatgaaac
     7261 acaccgtatc cgcatcggtc atcctgcttt tgaccgcttg cgcgcaatta cctcaaaata
     7321 acgaaaacct gtggcagccg tccgaacaca tcagcagttt tgcagcggaa gggcggttgg
     7381 cagtcaaagc ggaagggaaa ggttcgtatg caaatttcga ttggacatac caaccgcccg
     7441 tggaaaccat caatatcaac acccctttgg gcagtacgct cggacagttg tgtcaagaca
     7501 gggacggcgc attggcagtg gacggcaaag gaaatgtcta tcaggcagaa agtgcggaag
     7561 agttgggcag gcagctggtc ggtttcaaac tgccaatcca atatctgcat atctgggcgg
     7621 aaggcaggcg tgtggcgggc gcgccttacc gcatccgttc agacggcata ttggaacaat
     7681 acggttggac aatcggcaga accgccgaca gtggggggca agtccgaacg ttgcaactga
     7741 ataacggaaa tttgaacatc aggctggttt tcaccgaaat tggaatgccg tctgaaaccg
     7801 aaaccccgga acgctgtgcg gcgcgcacga gataaggcgg acagatgaat attgcggacg
     7861 gacggcaggc gtttcccgca cctgcaaaac tgaatctcga tttgaggatt accggcaggc
     7921 gggaagacgg ttatcacaat atcgaaagca tattctgcct gatagatttg caggataccg
     7981 tatatttgaa accgagggac gacggcaaaa tcatcctgca caatcctgtc ggcggaatac
     8041 cgcaggaagc cgatttgagc taccgtgccg catcgttgct gcaaaaatat gcgcgcaacc
     8101 ttgccggcgt ggaaatatgg ctggacaaaa aaatcccgac cggcgccggc ttgggagggg
     8161 ggagttccga tgccgcaacg gttttgctgg tgttgaaccg ttggtggcag tgtggtttga
     8221 cgcagtggca gctcattgat ttgggtgcgg ctttgggtgc ggacgtaccg ttttttattt
     8281 tcggaaaaaa tgcttttgca agcgggatag gtgaaaaact gataggaatg gatattccga
     8341 aacagtggta tgttattgtc aaacctcccg tccacgtttc caccgcaaaa attttcacat
     8401 acgaaggctt gacacgggat tctgcctcaa gcataatgcc gactttccaa aacctgcaac
     8461 cgttcagaaa cgatatgcag gcagtggtat ttaaagaata ccctgaagtt tggaaagctt
     8521 attccgagtt gtcgaaatac ggttctgcaa tgatgaccgg ctcaggagcc tgtatatttg
     8581 cagcatttca agctaggaat agcgcataca atatataccg acaagtttca ggtttgtacg
     8641 aggcatattt ggcagagggt ctttcaaaac atcctttgtt gtccgtataa acattgttgg
     8701 ggagtcgtca agcggttaag acactggatt ttgattccag catgcgaagg ttcgaatcct
     8761 tcctccccag ccaagccaaa caagttgggg agtcgtcaag cggttaagac actggatttt
     8821 gattccagca tgcgaaggtt cgaatccttc ctccccagcc agacaaaagc gtgtaagcct
     8881 gcttacacgc tttttatttc atagaaataa aaatattgaa acgcctttgt ttgtgtcgga
     8941 tgttgcaggt ataatgccgg gcttggtaca agcggaggga agcattgtgt tttctgagcg
     9001 gaagttaaac ataaaatcag gtgagaatat ggctgcgtac gacagtttga tggtatttac
     9061 aggcaatgcc aatcccgaat tggcacaacg tgttgtcagg catttggaca tttctttggg
     9121 caatgcttcc gtatccaagt tttcagacgg cgaagttgcc gtcgaactgt tggaaaacgt
     9181 acgcgggcgc gacgttttca tccttcagcc gacctgtgcg ccgaccaatg acaacctgat
     9241 ggaaatcctg acgatggcgg atgcactgaa gcgtgcttcg gcaggtcgta ttaccacagc
     9301 cattccgtat ttcggctatg cgcgccaaga ccgccgtccg cgttccgtcc gcgttccgat
     9361 ttctgccaaa ctggtggcaa atatgctgta ttcggcaggg atcgaccgtg ttttgactgt
     9421 cgatttgcat gccgaccaga ttcaaggttt cttcgatatt ccggtggaca atatttatgc
     9481 caccccgatt ttgttgaacg acatcaagca gcagcggatt gaaaatctga ccgtcgtcag
     9541 cccggacatc ggcggtgtcg tccgcgcccg cgccgttgca aaatccctga atgccgactt
     9601 ggcaatcatc gacaaacgcc gcccgaaagc caatgtggcg gaagtcatga acatcatcgg
     9661 cgatattcaa ggcaaaacct gtctgattgt ggacgatatg attgacactg caaatacgct
     9721 gtgcaaagcc gccgtcgcct tgaaagagcg cggtgcagaa cgtgtcttgg cttatgccag
     9781 ccacgctgta ttctccggag aggcggtcag ccgtattgcc tcatccgaaa tcgaccaggt
     9841 ggtcgtaacc gataccattc ctttgtctga agcggctaaa aactgcgacc gtatccgtca
     9901 ggtaacgatt gcaggtttgt tggccgaaac cgtccgccgc attagcaatg aagaatccgt
     9961 ctcatatctt ttcaatgaag aagtgatgac aggcagcatg ttgctgccgt aaccccgatg
    10021 ccgtcttaag ctggtcgcgg ccgatgacgg cgattttacc taacttggag tatttaacat
    10081 gacttatgaa attcaagcct ctgttcgtga agcacaaggc actggtgcga gccgccgcct
    10141 gcgtcgcgaa ggccaaatcc ccggtattct gtacggtgaa ggtcaagagc ctgttgccat
    10201 tgccgtagat cacaaaaccg tattttacgc attggaaaaa gaatctttcc atactgcgtt
    10261 gattaaattg tctctgaacg gcgaaaccaa agacgttatc gtccgcgatt tccaaatgca
    10321 cccgttccgc cgcgaagtcc aacacatcga cttccaagct gtgaaagccg atcaacttgt
    10381 acgcatccgc gttcccctgc acattgttaa cgctgaaaat tctcaagccg ttaaattgca
    10441 aggcggtcgc gtatctctgc tgaacacggc tgttgaagtg gttgcgttgc ctgccaatat
    10501 ccctgctttc ttggatttgg attgtgccga agtggttgcc ggcgacattt tgcacttgtc
    10561 agacatcaaa ctgcctgaag gtgtagaaag cgtttccctg aaacgtaacg aaaatctggc
    10621 tgttgctacc gttaccggta aaaagcgcta acttacttcc atcagcaggg cggcgcgtat
    10681 gcaatacgta ccgccctgtt gttttatgcc gtctgaaccg tgtttcagac ggcatttctt
    10741 tatttgttgg aaaagcggga tatttgaaac ggcagattac tgccctgtca gacgcgtcca
    10801 aagcctttgc caccggcttc ttttttctac gttttccagt gcgacgattt ctttttcggc
    10861 aatggtatgt ccgttttgcc tgattttgat ttttcctaaa atctgccctt tttttaccgg
    10921 ggcgggaatc ggctgtatgg tttccaaaat ctgttctgcc attttcgctt ctttatgtgg
    10981 cagagtgatg taggcttctt tgaggaagcc tgcgcggacg gtttttttgc tgcctccgga
    11041 aatttggatt tgggcaacgg ttttgccttt cggatatatt ttgggcgtat cgaaggcctg
    11101 caatgcccgg ttcagcagct tgctgttgtc cgatgcgcgg gtttccgccg attccgaacc
    11161 tagtgtgatg acaaggatgt gcctgccgtt gccggagtat gacacggcaa ggttgtagcc
    11221 gccgctttct gtgtgcccgg ctttcaggcc gtttacattg ttgtccctat ataaaaggat
    11281 attgcggttg ttttgttcta tgttttcaaa cttgaacgat ttgatggaaa acagcgggta
    11341 atattccgga aagtcgcgca tcaatgcttc agacagcagg gagaggtctt tggcggtgga
    11401 aacctgtcct tctctaccca agcctgtcgg gtttttgaat acggtgttct tcatgcccaa
    11461 gcgtcgggct tctttgttca tttgttgcac aaaattttca atcgagccgt tgcccagccg
    11521 gtcggcaagg gttagggcgg catcgtttgc gcatagggca atcatgcctt ttaagagttt
    11581 gtcggtgctg accgtatcgc cgggacgtac aaacattctg cttccttctg aagcccatgc
    11641 ggattcgggt atttttaagt tttcttcaga ttggatattt cccgatttca tgtttttgaa
    11701 aaccaaatat gcggtcatca gttgggttag tgccgccggt tcgacagggg tattggtgtt
    11761 tttggcggat aaagtctgcc tgctttgaag gtcgataacg atgtgtgccg ccgtgagggt
    11821 ttcgggtgtt tggagcgtgg gggcggcgtg taccgtcggt ctgttgggcg cgggcgatgc
    11881 agccgttgcg tgagaaacgc ctaagatgat gggaagaagg acgggcagga ttttatgtgc
    11941 tgtcatgaaa tattctaaat tgtgtgagtg tttcagcctg ccgattatac gcttagggtg
    12001 tctgatcggg cggatttttc ttgctttcgc gccgtcttgg gcgtatggtt ttgggttttg
    12061 cgattttaat aaaccgatta tcccgcattg aattatgaac acgccccttc cttattccga
    12121 ttacctcatc cgcatcctga cggcatctgt ctatgatgta gcggtcgaaa cgcctttgga
    12181 accggcacgc agcctttctg tacgtttgaa aaacaacatc cttttgaaac gcgaagattt
    12241 gcagccggtt ttttcgttca aaatacgcgg cgcgtacaac aaaatgtcca agttgccgaa
    12301 agatgcgctc gcttgcggcg tgattgcggc aagcgcgggc aatcacgcac aaggcgtggc
    12361 gttgtccgca cagcgtttgg gctgccgtgc cgttatcgtc atgccggaga ctacgccgaa
    12421 aattaaagtg gatgcggtta aaagccgtgg cggcgaggtg gttttgcggg gtgtttcata
    12481 caacgatgcc tacgattatg cgatggagct ggcggaacag gaagggctga cctatatcgc
    12541 gccgtttgac gatcccgatg tgattgcggg acaggggacg gtggggatgg aaattgtcag
    12601 ccagcatccc gatccaatcc gcgccgtatt cgtaccgata gggggcggcg gtttggcggc
    12661 gggcgtggcg gcatttatca aacaggttcg tcccgaaatt aaagttatcg gcgtacagac
    12721 caacgattcc tgctgtatga agcagtcggt cgaagcgggc gaaatcgtcc atttgaaaga
    12781 tgtcgggctg ttttcagacg gtacggcggt caaagtcgtc ggaaacggaa ccttccgcct
    12841 ctgcaaagaa cttttggatg aaatcattac agtcgatacc gatgcggttt gcggcgcggt
    12901 caaggatatt ttcgatgaca cgcgcagcat taccgagccg gccggggcgt tggcgttggc
    12961 gggtctgaaa gcctatattg cccgagaagg tgcggaaaac caaaccctga ttgccgttac
    13021 cagcggtgcg aatatgaatt ttcaccgttt gcgccacgtt tcggaacgga gcgaattggg
    13081 cgagggcaac gaaggtattt ttgccgttac catccccgaa gaacgcggca gcttccttaa
    13141 gtttgtcaat atattgggaa ataggaatat taccgagttc aactaccgct acggcgacga
    13201 tgaaaaagcg catatctttg tcggacttca agcggcaggc ccgcaggatt tggcggttat
    13261 cggcagccgg ttggatgagg cgggattgcc caatgtcgat ttgaccaata atgagattgc
    13321 caaaatccat atccgctata tggtcggagg gcggacggac aaagtagggc acgagcgtct
    13381 ggtcagtttt gagtttccgg agcgtccggg cgcgttggcg cgctttttga accatatgca
    13441 gggcggttgg aacattacgc tcttccatta ccgcaaccac ggtgcggatt acgggcggat
    13501 tttggtcggt atcgacgtgc cgccgcacga tgccgccgca tttgacggtt tcttggaaag
    13561 tctgggatac agctatcacg aggaaacgca aaatgccgcg tacaagctgt ttcttgcctg
    13621 acgtttgaca cgcaatgccg tctgaaagcc tttcagacgg cattgcgctt tcagggttaa
    13681 atcgaatatt caatcaactc gttttgagaa aaaacataaa cctgtttcgg aattaagttt
    13741 aattttttgc cttcggcgat tgggtagcgc gcggcatcgc tgccagccag tgtgatatgc
    13801 acgtcttgtt tgccgtgttt caccaaaacg tgcgtcaatg cgccgacggc gtggattttt
    13861 tcgatttcgg cacggatcat cggggtttcg tgttcggcgg cgatctgcca ttcgtgcggg
    13921 cggatatagc cggtggcggt ttgctcctgc catttgtatt gcgcgtccaa tttccacgcg
    13981 aagccgttgt aatgccagat ccctttttcg atgcgtcctt caaatgcgtc ggcttcgccg
    14041 aggaactcgg taacgaaggc gttttcgggt ttgcggtaaa tggcttcggc actgccggtt
    14101 tgttcgattt tgccatggtt catcacgacg atttcgtcgg aaacttcgag ggcttcttct
    14161 tggtcgtgag tcaccagaat gctggttacg ccgaggttgt gatgaatgtc gcgcagccag
    14221 gtgcgtaatt ctttgcgcac tttggcatcc agcgcgccga agggttcgtc caaaagcagg
    14281 agtttcggtt cgaccgcaag cgcgcgggcg agggcgatgc gctggcgttg cccgccggag
    14341 agttggtgcg gataggattt ggcgaggtgg gagagttgta cgagtttgag caattcttcg
    14401 actttggcgc ggatttgtcc tttggacggg cgttcgggct tgggcaatac ggtcaaaccg
    14461 aaagcgacgt tgtcaaacac attcatgtgg cggaagaggg cgtagtgttg gaacacgaaa
    14521 ccgactttgc gctcgcgcac atgcttggcg gttacgtctt ggccgtcaaa gagaatttgg
    14581 ccgccgtcgg cgttttccag tccggcgata atgcgtaaaa gtgtggtttt gccgcagccg
    14641 gagggaccga gcagggaaac gagtttgccg gcggggacgt tgaggttgat gtttttcagc
    14701 gcgtgaaaat tgccgaagcg ttttttgttt aagttttgaa tggtaatact catactgcgt
    14761 tcctttcggc ggcggcgagt tttttgtctt gtatagtgga ttaaatttaa accagtacgg
    14821 cgttgcctcg ccttgccgta ctatttgtac tgtctgcggc ttcgtcgcct tgtcctgatt
    14881 taaatttaat ccactataat ttggtgatga tgttctgcac cgccagcgtc gccagtgcta
    14941 aaagtgccaa tacgccggag agggagaatg cgccggtgaa gttgtattcg ttgtagaaga
    15001 tttcgaccaa aagcgggata gtgttggttt cgccgcgtat gtgtcccgat accacgctga
    15061 ccgcgccgaa ctcgcccatc gcacgggcgt tggtgaggat gatgccgtag agcagcgtcc
    15121 atttgatgtt gggcagggta acgcgccaaa acatctgcca accgcttgcg ccgagtacca
    15181 atgccgcctg ttcttcgctg tcgccctgtg tctgcattaa agggatgatt tcgcgtgcgg
    15241 caaagggaaa ggtaacgaac agcgccgtca aaacaatgcc gggaatggcg aagataatct
    15301 gtatgccttg cgcttccagc cggctgccca acgccgtgtg cgcgccgaac aataagacga
    15361 acatcaaacc ggcaaccacg ggcgatacgg aaaacggcaa atcgagcagg gtggtcagca
    15421 actgcttgcc gagaaagtca aaacgggtca acagccacgc catcgccacg cccaagacgg
    15481 cattgacggg aacggcaatc agcgcggtaa tcagcgtcaa tttgacggca gaccacgctt
    15541 cgggatcgct taaggatttc aggtacaaat cccaaccgcc ttttaaggct tcgtagaata
    15601 cggcgacgag cggcacgacc agcatcagca gcagaaagcc cagcgcggcg gcaatcagca
    15661 acatgcgcag ccggcgcggt tcggtcaggt tgggattggc ggaatagggt ttcatggcgg
    15721 tggtgttctc tggtttgttg tttaaggtgt tcggatgccg tctgaaaggg attgaggggt
    15781 ttcagctttc gtcggtcgga ttcttgaatc cgacaaggtt gctgaatcgc aaatgtttgt
    15841 cggatacaag tatccgacct acgaattctg tgttttgggg agcggattcg ttttcagacg
    15901 acctcaaccc ttcgcacccg aacgcctgcc caacgcccac tgcatcacgt tcagcgcaaa
    15961 cagaatcaca aacgaaacca gcagcataaa caacgccacc gccgacgcgc cctgcgcgtc
    16021 gaattgttcc agcttgcccg taataatcag cggcaggatt tcggaaatca tcggaatgct
    16081 gcccgcgata aaaatcaccg aaccgtattc ccccgttgcc cgcgcgaaca tcatgcccgc
    16141 gccggtcaag agtgccggcg tgatttccgg cagcagcacg cggcggaacg ttgtgaaacg
    16201 gtttgcgccc aaagtcgccg ccgcttcctc atattcgccc gacaattcct ccaataccgg
    16261 ctgcacggcg cggacgataa agggcaggct gacgacaacc agcgcaatcc aaatgccgac
    16321 gggcgtaaac gcgattttga tgcccaaagg ttcgaaaaaa cggcctatcc aaccgttggg
    16381 cgcatacagg gccgccaacg cgatacccgt aaccgccgtc ggcagcgcaa acggcaaatc
    16441 aaccagcgcg ttcgccaggc ccttgcccgg aaattcatag cgcaccaaca cccaagccac
    16501 cagtgtgccg aacacgacat tggtcagcat cgcataaaac gacatccgca aactcagccg
    16561 caccgccgcc agcacgttcg gctcggcaat cgtgttccga aagccgtccc agccgatttc
    16621 cgccgccttc gccgccatca tcgcaaacgg caaaaccacc agaagcgaca ggcacaatac
    16681 ggtcagaccg aggctgagtt tgaagccggg cagtacgccg ggtgttttga gcggtaacat
    16741 ggtcggtaag ggtggaaaat aaaggcatta ctttaacaat gccgcctgaa aaacggaaaa
    16801 aatggaaagt ttggtgcaaa gacgaatttg ttataaagcg gttggcagtt tcttaagcgg
    16861 gcgcgatgtt ctaaaataac gctttgtttt gacggaaaac catcatataa aggaacactt
    16921 atgcagattt tatcttttca ggcggacatt gcggaacgta tgctggaagg tacggaaggc
    16981 gagtcggtca acgaaaacgc acaatttgtc cgtacggaca acggctattg gattgcgtgg
    17041 catgaaggcg tggcggcact gcttgcgccc gatacgccgc cgggcattcc ctgtttttgg
    17101 gtggaagggg cggaaagcct tgaagagttg tgcgcgatgg tggaacgcgg cgagtttgac
    17161 gaagtggaag agtttgacgg cgatgacgac gcgtggctcg aagcggctaa agattgcgga
    17221 caccacggcg acgcttgcgc ctgcggacat taaaggcatg gcaggcttgc cgcaaggggt
    17281 gcaaggcttt gccgtttttt aaataatcgg cggattgcat ctgtttgcaa tgggcggaac
    17341 gcttcccttt gtattttatt gaataaaaat tatcttggta ttataataag gcagcatcaa
    17401 ttattttggg actgcaacaa acgcaaagca ttgatttgcg tttgttgcaa acttattatt
    17461 atagcaggtt gcggcgcgga cttgatggga attgtttata gagcatgata gtctgaaaca
    17521 tcattaatta aaaataccat taatccgatt tatatttatt tcaatttcaa tggaaaaaca
    17581 tcaatgacaa tgattttaag cattttaagc ctgtttttta tcattagact gttattttta
    17641 gccgtctcca ttaaacatga aaaagccttg attgccaaag gggcgaaaca atacggaaaa
    17701 accaattcca cggtgcttgc ggcagttcat acgctttatt atttggcgtg ttttgtttgg
    17761 gtatggcttt ctgacactgc ttttaatggc atatccttga ttggtacact gacggtgatg
    17821 gcttcatttg tgatattatc attgattatt aagcagttgg gggagatttg gacggttaaa
    17881 atctatattt tgccaaatca tcaaattaat cgttcgtggt tgtttaaaac attccgccac
    17941 ccgaattatt ttttaaacat catacccgaa ctgattggca tcgccttatt atgtcaagcg
    18001 tggtatgttt tattgattgg cctgcccatt tatttgctgg tcttatttaa gcgtatccga
    18061 caagaagaac aggcgatggc aacacttttt taacccgttt catcaattaa aaatatatcg
    18121 acaaagagaa ccgtctatca attaaatcca ttcttatcat tggggcaggc ttattgtcat
    18181 tgggcaagcc gtgacaaaag cccgaacctg caaaacttat ctataaggct gcatcagtgc
    18241 gtattgaagc cccgcctttt ttgacaaaaa aatgccgtct gaaggcttca gacggcattt
    18301 gttcggtttg gcaaagccgg cggcttatgc ggcaaaacgt ttggcgactt cgtcccagtt
    18361 gacgatttcc caaaaacctt ccaggtagtt ggggcggctg ttgcggtagt cgatgtagta
    18421 ggcgtgttcc cacacgtcgc aagtcaagag cggcgtgttt tcagtggtca gcggcgtagc
    18481 ggcgttggaa gtggaaatca aatccaatcc gccgacaggg gttttcacca gccacgccca
    18541 accggagccg aacgtaccgg ccgcgcaggc attgaacgct tcttggaatt tttcgaagct
    18601 gccccacttc gcgtcgatgg cggcggccag ttcgccggca ggcttgcctt ggcctttggg
    18661 cgtgaaaccc agccagtaga aggtgtggtt ccaagtttga gccgcgttgt tgaacacgcc
    18721 gcccgaagat tttttcacaa tctcttccaa aggcaggttt tcaaattcgg tgcctttgat
    18781 ttgattgttc aggttggtga tgtaggtttg atggtgtttg ccgtagtgga attccaaagt
    18841 ctctttgctc agatgcgggg acaatgcgtc cggctcataa ggcagttgcg gcagcttatg
    18901 ttccattttg tgctcctaat gttgtttttg gatgttgtgt tttggcagtg ttgctgcaaa
    18961 cgaatcggcg gctcacgcat tctacctgtt ttgagtgatg gaaaccaatt aaacctgctt
    19021 tgcgttataa tagaagattg caatttcggc atgacagatt ggatgtacca tgaacgatta
    19081 cgcagctatg ccgcctgaag accgtgaggt cggcgcattg tcgctgcctc cgcattcgat
    19141 ggaggcggaa caatccgttt tgggcggttt gatgctggaa aatccggctt gggacaggat
    19201 tgccgatgtg gtttcgggag aggatttcta ccgccacgaa caccgcctga ttttccgatc
    19261 cattgccaaa ttaatcaatg agggccgtcc cgcagatgtg attacggttc aggaagattt
    19321 gcagcggaac gaagaattgg aagcggcagg cggatttgaa tatctgatta cactggcgca
    19381 aaacaccccg tctgccgcaa acatccgccg ctacgccgaa atcgtacgcg agcgttccat
    19441 tatgcgccaa ctcgccgaag tggggacgga aatcgcccgc agcgcataca atccgcaagg
    19501 cagggacgcg gggcggcttt tggatgaggc ggaaaacaaa gtgtttcaaa tcgccgaaag
    19561 caccgccaaa tccaagcagg gctttttgga gatgcccgat ttgctgaaag aagtcgtaca
    19621 gcgcatcgat atgctttatt cgcgcgacaa tcccgatgaa gttaccggcg taccgacagg
    19681 gttcatcgac cttgataaaa aaacctcggg tctgcagccc ggcgacctga ttatcgtcgc
    19741 cggccgcccg tctatgggta aaaccgcgtt ctccatcaat attgccgagt atgtcgccat
    19801 tgaaaaacat ttgcccgtcg ccgttttctc gatggaaatg ggcggggcgc agttggtaat
    19861 gcgtatgctc ggttcggtcg gacgcttgga tcagagcgtc ctgaaaacag gcaggctgga
    19921 agatgaacat tgggggcgtt tgaacgaggc agtcgtcaaa ctttccgacg cgcctatgtt
    19981 tattgacgaa actccgaatc tgaccgccct cgaattgcgc gcccgcgccc gccgcctcgc
    20041 ccgccggttt gacggcagat taggcttgat cgtcatcgac tacctgcaac tgatggcggc
    20101 atccggccgt tcggacaacc gcgcttccga gcttggcgaa atttcccgtt ccctcaaagc
    20161 cctggcaaaa gagttgcaag tccccatcat cgccctgtcg caattgagcc gcactgtcga
    20221 acagcgcacc gacaaacgcc cgatgatgtc cgacctccgc gaatccggtg ctatcgagca
    20281 ggatgccgac ctgattatgt ttatgtaccg cgacgaatac tacaaccaag aatcgcagat
    20341 gaagggttat gcggaatgca tcatcggcaa acaccgcaac ggtcccgtcg gtaaaatctt
    20401 cctcacatgg acgggacaat tcaccaaatt cgacaatgct gcctatattc ccgaggaggc
    20461 aaagatagaa gattaaatgg ctatataaaa atttattagg cggaatcagg caaaatcgtt
    20521 taaaatcatg cttagagatt gccctaaaaa ataaaacgcg gtcttgaggc atttttgcat
    20581 tcagcccgca tataattgaa aatatagtgg attaacaa
//
LOCUS       DAWTQS010000034        20446 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.034, whole genome shotgun sequence.
ACCESSION   DAWTQS010000034 DAWTQS010000000
VERSION     DAWTQS010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20446
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.034"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            53..322
                     /locus_tag="WNA25_001224"
     CDS             53..322
                     /locus_tag="WNA25_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823977.1"
                     /translation="MSVKELFKVILDKNKDFPIRTIHRTPMGILPKPVALSIVQYEND
                     PGFYLFYLDETGQEQTDTYHDTLDSAFEQAEFEFGISKEEWMQSP"
     gene            304..456
                     /locus_tag="WNA25_001225"
     CDS             304..456
                     /locus_tag="WNA25_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823978.1"
                     /translation="MDAKSLDGKIKMPLYFSDGISVNKKVGIGAQNSLRLAGIGMKSL
                     RKNDYS"
     gene            443..988
                     /locus_tag="WNA25_001226"
     CDS             443..988
                     /locus_tag="WNA25_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HFA9823979.1"
                     /translation="MIIVEHLEHYLGEIESGIKCLDRRYHLSVSVFPSQPYKGVTTFS
                     TLGLNRYDLNYKSRFELIFTCSEEWNKENIAAFLSGVAEYLIDNRQPILRGEIIQLPR
                     VIIEGSKMDALYVSAPFYFDDDFQVCYGEHYNIVFPLLVPLYKQEAELVEKKGWNAFE
                     QFLLDNEVDNLSDMKRKPFAW"
     gene            1392..1793
                     /locus_tag="WNA25_001227"
     CDS             1392..1793
                     /locus_tag="WNA25_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823980.1"
                     /translation="MKIKPLQFSNNNHRFYVDKICVTEQDVNILASDRNYELNIEIFI
                     DNIIHFQITDESYKVKFSEYLFENKTENNSDGNPTANYFFEIIDDSYMDWLKEESFGF
                     FEKKYYKAYIFFFSDSVIEVISSTEPVFYSK"
     gene            complement(1936..2043)
                     /locus_tag="WNA25_001228"
                     /pseudo
     CDS             complement(1936..2043)
                     /locus_tag="WNA25_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            2065..2559
                     /locus_tag="WNA25_001229"
     CDS             2065..2559
                     /locus_tag="WNA25_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823981.1"
                     /translation="MENRFEKIVKDIFSFTQFVSEAENKIPNQSSRYYDCWWELEILN
                     ASALEDWEISGKPEKWEKWTEKYQTEALQSVKDLFAEEVPENNIVCDISHFLHFADRA
                     EADIPNQSRLYAEYWWEMETFKMSVLKDWERSGRPLDWEKWQRQYQSEAFELVKNLNR
                     SKQE"
     gene            2679..3812
                     /locus_tag="WNA25_001230"
     CDS             2679..3812
                     /locus_tag="WNA25_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823982.1"
                     /translation="MFKKFKPVLLSFFALVFAFWLGTGIAYEINPRWFLSDTATEIPE
                     NPNTFVAKLARLFRNADRAVVIVKESMRTEESLAGAVDDGPLQSEKDYLALAVRLSRL
                     KEKAKWFHVTEQEHGEEVWLDYYIGEGGLVAVSLLQRSPEAFVNAEYLYRNDRPFSVN
                     VYGGTAHGENYETTGEYRVVWQPDGSVFDASGRGKIGEDVYEHCLGCYQMAQVYLAKY
                     RDVANDEQKVWDFREESNRIASDSRDSVFYQNMRELMPRGMKANSLVVGYDADGLPQK
                     VYWSFDNGKKRQSFEYYLKNGNLFIAQSSTVALKADGVTADMQTYHAQQTWYLDGGRI
                     IREEKQGDRLPDFPLNLEDLEKEVSRYAEAAARRSGGRRGLSH"
     gene            complement(4315..4590)
                     /locus_tag="WNA25_001231"
     CDS             complement(4315..4590)
                     /locus_tag="WNA25_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFA9823983.1"
                     /translation="MNASNWSVYLILCENSAFYCGISPNPQQRLAIHTAGKGAKYTRV
                     FKPVAMRIVAGGMDKGTALKQEIAVKKLTAAQKRQLWEQGEKMPSET"
     gene            complement(4587..5870)
                     /locus_tag="WNA25_001232"
     CDS             complement(4587..5870)
                     /locus_tag="WNA25_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFA9823984.1"
                     /translation="MTASKSGFIGQIFSRNMLVCIFTGFASGLPLYFLINLIPAWLRS
                     EQVDLKSIGLMALIGLPFTWKFLWSPLMDAVRLPVLGRRRGWMLLTQAGLLAALAAYA
                     FLNPRNHLPLIAGLSVLVAFFSASQDIVLDAFRREILSDEELGLGNSVHVNAYRVAAL
                     IPGSLSLVLADRMPWSEVFVITSLFMLPGLLMTLFLAHEPVLPPSVPKTLKQTVVEPF
                     KEFFTRKGIASAVCVLLFIFLYKLGDSMATALATPFYLDMGFSKTDIGLIAKNAGLWP
                     AVAAGILGGVWMLKIGVNKALWLFGAVQAITVLGFVWLAGFGHFDTVGTGERLMLAAV
                     IGAEAVGVGLGTAAFVSYMARETNPAFTATQLALFTSLSAVPRTVINSFAGYLIEWMG
                     YVPFFRLCFILALPGMLLLLKVAPWNGEKTQDAGR"
     gene            complement(6019..7437)
                     /gene="glnA"
                     /locus_tag="WNA25_001233"
     CDS             complement(6019..7437)
                     /gene="glnA"
                     /locus_tag="WNA25_001233"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFA9823985.1"
                     /translation="MSIKNAVKLIEESEARFVDLRFTDTKGKQHHFTVPARIVLEDPE
                     EWFENGPAFDGSSIGGWKGIEASDMQLRPDASTAFVDPFYDDVTVVITCDVIDPADGQ
                     GYDRDPRSIARRAEAYLKSSGIGDTAYFGPEPEFFVFDGVEFETDMHKTRYEITSESG
                     AWASGLHMDGQNTGHRPAVKGGYAPVAPIDCGQDLRSAMVNILEGLGIEVEVHHSEVG
                     TGSQMEIGTRFATLVKRADQTQDMKYVIQNVAHNFGKTATFMPKPIMGDNGSGMHVHQ
                     SIWKDGQNLFAGDGYAGLSDTALYYIGGIIKHAKALNAITNPSTNSYKRLVPHFEAPT
                     KLAYSAKNRSASIRIPSVNSSKARRIEARFPDPTANPYLAFAALLMAGLDGIQNKIHP
                     GDPADKNLYDLPPEEDALVPTVCASLEEALAALKADHEFLLRGGVFSKDWIDSYIAFK
                     EEDVRRIRMAPHPLEFEMYYSL"
     gene            complement(7438..7653)
                     /locus_tag="WNA25_001234"
                     /pseudo
     CDS             complement(7438..7653)
                     /locus_tag="WNA25_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272104.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
     gene            7748..8557
                     /gene="aroE"
                     /locus_tag="WNA25_001235"
     CDS             7748..8557
                     /gene="aroE"
                     /locus_tag="WNA25_001235"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFA9823986.1"
                     /translation="MHALPRYAVFGNPAAHSKSPQIHQQFALQEGVDIEYGRICADIG
                     GFAQAVSTFFETGGCGANVTVPFKQEAFHLADEHSDRALAAGAVNTLVWLEDGRIRGD
                     NTDGIGLANDITQVKNIAIEGKTILLLGAGGAVRGVIPVLKEHRPARIVIANRTRAKA
                     EELARLFGIEAVPMADVNGGFDIIINGTSGGLSGQLPAVSPKIFRDCRLAYDMVYGEA
                     AKPFLDFARQSGAKKTADGLGMLVGQAAASYALWRGFKPDIRPVIEHMKAL"
     gene            8560..9261
                     /gene="mtgA"
                     /locus_tag="WNA25_001236"
     CDS             8560..9261
                     /gene="mtgA"
                     /locus_tag="WNA25_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFA9823987.1"
                     /translation="MFRIVKWLIALPVGIFIFFNAYVYGNIITYRAVAPHRTAFMSMR
                     MKQFEQEGRDVALDYRWMPYKRISTNLKKALIASEDVRFAGHGGFDWGGIQNAIRRNR
                     NSGEVKAGGSTISQQLAKNLFLNESRNYLRKGEEAAITAMMEAVTDKNRIFELYLNSI
                     EWHYGVFGAEAASRYFYKKPAADLTKQQAAKLTALVPAPLYYADHPKSKRLRNKTNIV
                     LRRMGSAELPESDTD"
     gene            complement(9535..9610)
                     /locus_tag="WNA25_001237"
     tRNA            complement(9535..9610)
                     /locus_tag="WNA25_001237"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9575..9577),aa:Phe,seq:gaa)
     gene            complement(9801..10535)
                     /gene="lptB"
                     /locus_tag="WNA25_001238"
     CDS             complement(9801..10535)
                     /gene="lptB"
                     /locus_tag="WNA25_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFA9823988.1"
                     /translation="MSANNSRLVVQNLQKSFKKRQVVKSFSLEIESGEVIGLLGPNGA
                     GKTTGFYMIVGLIAADAGSVMLDGRELRHLPIHERARLGVGYLPQEASIFRKMTVEQN
                     IRAILEIRTKDKNQIDREIEKLLADLNIGHLRRNPAPSLSGGERRRVEIARVLAMKPR
                     FILLDEPFAGVDPIAVIDIQKIIGFLKSRGIGVLITDHNVRETLSICDRAYIISDGAV
                     LASGKPDDLVGNEQVRAVYLGENFKY"
     gene            complement(10606..11136)
                     /gene="lptA"
                     /locus_tag="WNA25_001239"
     CDS             complement(10606..11136)
                     /gene="lptA"
                     /locus_tag="WNA25_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HFA9823989.1"
                     /translation="MIQKICKLFVLIVIFATSPAFALQSDSRRPIQIEADQGSLDQAN
                     QSTTFSGNVIIRQGTLNISASRVNVTRGGKGGESVRAEGSPVRFSQTLDGGKGTVRGQ
                     ANNVTYSSAGSTVVLTGNAKVQRGGDVAEGAVITYNTKTEVYTINGSTKSGAKSASKT
                     GRVSVVIQPSSTQKTE"
     gene            complement(11117..11698)
                     /gene="lptC"
                     /locus_tag="WNA25_001240"
     CDS             complement(11117..11698)
                     /gene="lptC"
                     /locus_tag="WNA25_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFA9823990.1"
                     /translation="MKVRWRYGIAFPLILAVALGGLSAWLGRISEVEIEEVRLNPDEP
                     QYTMDGLDGRRFDEQGYLKEHLSAKGAKQFPENSDIHFDSPHLVFFQEGRLLYEVGSD
                     EAVYHTENKQVLFKNNVVLTKTADGRRQAGKVETEKLHVDTESQYAQTDTPVSFQYGA
                     SHGQAGGMTYNHKTGMLNFSSKVKAAIYDTKDM"
     gene            complement(11695..12231)
                     /locus_tag="WNA25_001241"
     CDS             complement(11695..12231)
                     /locus_tag="WNA25_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFA9823991.1"
                     /translation="MQAISPELQARAAKIKLLILDVDGVLTDGRIFIRDDGEEIKSFH
                     TLDGHGLKMLQASGVQTAIITGRDAPSVGIRVKQLGINYYFKGISDKRAAYEELRAQA
                     GLEEAECAFVGDDVVDLPVMVRCGLPVAVPDAHWFTLQHAAYIAKRPGGAGAVREVCD
                     LIIRAKGTLGAALNGYIK"
     gene            complement(12297..13271)
                     /locus_tag="WNA25_001242"
     CDS             complement(12297..13271)
                     /locus_tag="WNA25_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HFA9823992.1"
                     /translation="MAENEKYLDWAREVLHTEAEGLREIAAELDENFVLAADALLHCK
                     GRVVITGMGKSGHIGRKMAATMASTGTPAFFVHPAEAAHGDLGMIVDNDVVAAISNSG
                     ESDEITAIIPALKRKDITLVCITARPDSTMARHADIHITASVSQEACPLGLAPTTSTT
                     AVMALGDALAVVLLRARAFTPDDFALIHPAGSLGKRLLLRVADIMHKGGGLPAVRLGT
                     PLKGAIVSMSEKGLGMLAVTDGQGCLKGVFTDGDLRRLFQECDNFTGLSIDEVMHTHP
                     KTISAERLATEALKVMQANHVNGLLVTDADGVLTGALNMHDLLAARIV"
     gene            13368..14423
                     /gene="tal"
                     /locus_tag="WNA25_001243"
     CDS             13368..14423
                     /gene="tal"
                     /locus_tag="WNA25_001243"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFA9823993.1"
                     /translation="MTILSDVKALGQQIWLDNLSRSLVQSGELAQMLKQGVCGVTSNP
                     AIFQKAFAGDALYADEVAALKRQNLSPKQRYETMAVADVRAACDVCLAEHESTGGKTG
                     FVSLEVSPELAKDAQGTVEEARRLHAAIARKNAMIKVPATDAGIDALETLVSDGISVN
                     LTLLFSRAQTLKAYAAYARGIAKRLAAGQSVAHIQVVASFFISRVDSALDATLPDRLK
                     GKTAIALAKAAYQDWEQYFTAPEFAALEAQGANRVQLLWASTGVKNPAYPDTLYVDSL
                     IGVHTVNTVPDATLKAFIDHGTAKATLTESADEARARLAEIAALGIDVETLAARLQED
                     GLKQFEEAFEKLLAPLV"
     gene            14574..14864
                     /locus_tag="WNA25_001244"
     CDS             14574..14864
                     /locus_tag="WNA25_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9823994.1"
                     /translation="MAKALEIISPDEIYSDLIFKDPVPPHTIYTELMKLVGRNAGNER
                     IADRAFDFFSPALMDDSATEEQYNALYDLTLLEEPGMELDKDEITALINSLK"
     gene            14881..15768
                     /gene="gluQRS"
                     /locus_tag="WNA25_001245"
     CDS             14881..15768
                     /gene="gluQRS"
                     /locus_tag="WNA25_001245"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFA9823995.1"
                     /translation="MYTGRFAPSPTGLLHIGSLLTAVASYADARSNGGKWLVRMEDLD
                     PPREMPGAASHILHTLEAFGFEWDGEVTYQSRRYALYEETLYRLKTAGLVYPCHCNRK
                     DWQAGARRGTDGFVYNGRCRHPGQRPALQGKQPSWRIRVPDRDIGFSDGIVGSYTQNL
                     ARDIGDFVLFRADGYWAYQLAVVADDAEQGVTHIVRGQDLLVSTPRQIYLQQCLGVPT
                     PQYAHLPLLTNAQGQKWSKQTLAPALDSNRREQLLRQVFRYLKLPEAPETDRPAELLD
                     WAVAHWDMDKVPKHAITAP"
     gene            complement(15838..16854)
                     /gene="dusA"
                     /locus_tag="WNA25_001246"
     CDS             complement(15838..16854)
                     /gene="dusA"
                     /locus_tag="WNA25_001246"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFA9823996.1"
                     /translation="MINKELIEHKGKRRLCVAPMLDWTDRHYRYLARQITRNAWLYSE
                     MVNAGAIVYGDKDRFLMFNEGEQPVALQLGGSDPSDLAKAAKAAEEYGYNEVNLNCGC
                     PSPRVQKGAFGACLMNEVGLVADCLNAMHDAVGIPVTVKHRIGVDRQTEYQTVADFVG
                     TLRDKTACKTFIVHARNAWLDGLSPKENRDVPPLKYDYVYRLKQEFPELEIIINGGIT
                     TNEAIAGHLQHVDGVMVGREAYHNPMVMREWDRLFYGDNRAPIEYADLVQRLYTYSQV
                     QIQAGRGTILRHIVRHSLGLMHGLKGARTWRRMLSDATLLKDNDGGLIFEAWKEVERA
                     NTRE"
     gene            16974..18128
                     /locus_tag="WNA25_001247"
     CDS             16974..18128
                     /locus_tag="WNA25_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFA9823997.1"
                     /translation="MATITKRRNPSGETVYRVQVRVGKKGYPAFNESRTFSKKALAVE
                     WGKKREAEIEAGPELLFKRGKVKMMTLSEAMRKYLNETLGAGRSKKMGLRFLMEFPIG
                     GIGIDKLKRSDFAEHVMQRRRGIPELDIAPIAASTALQELQYIRSVLKHAFYVWGLEI
                     GWQELDFAANGLKRSNMVAKSAIRDRLPTTEELQTLTTYFLRQWQSRKSSIPMHLIMW
                     LAIYTSRRQDEICRLLFDDWHKNDCTRPVRDLKNPNGSTGNNKEFDILPMALPVIDEL
                     PEESVRKRMLANKGIADSLVPCNGKSVSAAWTRACKVLRIKDLRFHDLRHEAATRMAE
                     DGFTIPQMQRVTLHDGWNSLQRYVSVRKRSTRLDFKEAMMQAQSDIKSGK"
     gene            complement(18178..18444)
                     /locus_tag="WNA25_001248"
     CDS             complement(18178..18444)
                     /locus_tag="WNA25_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyocin activator PrtN family protein"
                     /protein_id="HFA9823998.1"
                     /translation="MNISKEQGLLIAYGGRPYIPPREVHKDFFAHIGFNAFKASGVRC
                     ELPFPIFRLSDSRKSEYFVSVRELAKAIADKEEKAKSEYRKFRS"
     gene            complement(18552..19430)
                     /locus_tag="WNA25_001249"
     CDS             complement(18552..19430)
                     /locus_tag="WNA25_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HFA9823999.1"
                     /translation="MRRLVAAKVRRYCGEHPGVFDGAAGSGQLEQYIEPSDFRAVEIQ
                     AEACKALLQNYPAAKVYNTSLFLYTDGEPQDCTVMNPPFSIKLKDLSEDEKSRIAQEY
                     PWKKSGVADEIFVLKGLENARRFGFFILFPGIAYRKSEQRFREIIGNRLAELNRIQNA
                     FEDTPIEVLLLVIDKDKTDGGCIRELYDCKTDTLLAADTWQIEPDLWQTVQEPAPPKE
                     KEDPVLLEHECRDAAAKRIARELRFSKMVNEIEGWPHAEFDGFCDRLCNLIQAEKYGK
                     KHYFPCSLPLFGGAAG"
     gene            complement(19471..19701)
                     /locus_tag="WNA25_001250"
     CDS             complement(19471..19701)
                     /locus_tag="WNA25_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824000.1"
                     /translation="MRTHIRTCVYHDSGTKGFKHGIRHKRHGCRRGGTSVFQRGEDGK
                     PQRVSRIRKRSATPGQARERTKTHDVSRTQQP"
     gene            complement(19698..20435)
                     /locus_tag="WNA25_001251"
     CDS             complement(19698..20435)
                     /locus_tag="WNA25_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824001.1"
                     /translation="MRYACNQELINFAEHIVKQVQRLGLYCNTDDPANEESVLFACRE
                     ASQAVAQWTKDFDDLSPNQRQLVLRPLSNLFAAYEEFLKDAPARLIAEVSAYSLAVRV
                     AKKAMAFLELDGGLISAVGKVVNGADSRAEARRLKMPYAEFTGRILHAANLLYDVGIQ
                     ADKELSAMYGKPLNPVRPRRISDVRRPMMKMLVADKGGALVRAVKDSEDVIRHCDNGA
                     GFSCFNWTEHFKRTANLISLMHREAAA"
BASE COUNT         4841 a         5269 c         5414 g         4922 t
ORIGIN      
        1 tggacgtgta atagaaaata gtgtagtaac aattattcag gattagaaaa ttatgagtgt
       61 aaaagaatta ttcaaagtta tcttagataa aaataaagat tttcctatta gaacgattca
      121 tcgcactcca atggggatac ttcccaaacc tgttgcttta agtattgttc aatatgaaaa
      181 tgacccggga ttttatttgt tttatttgga tgagaccggt caggaacaaa cggatactta
      241 tcatgacaca ttagattcgg catttgaaca ggctgaattt gaatttggaa tcagcaaaga
      301 ggaatggatg caaagtcctt agacggcaaa atcaagatgc cgctttattt ttcagatggc
      361 atttccgtca ataaaaaagt cggtattgga gcccaaaaca gcctacgtct tgccgggata
      421 gggatgaaat ctttaagaaa aaatgattat agttgaacat ttagaacatt atttgggtga
      481 aatcgaaagc ggtataaaat gcttggatcg acgttatcat ctcagcgttt ccgtcttccc
      541 atcacaacca tataaagggg taactacttt ttctacttta ggcttgaatc gatatgactt
      601 gaactataag agtaggtttg aattaatttt tacctgttct gaagaatgga ataaagaaaa
      661 tattgcagct tttctatcag gagtggctga atatttgatt gacaatagac aacctatttt
      721 aagaggtgaa attattcaat tacccagggt aattatcgaa ggtagcaaaa tggatgcact
      781 gtatgtttcc gccccatttt atttcgatga tgattttcag gtctgttatg gcgaacacta
      841 caatatcgtt ttccctttgc ttgtcccctt gtataaacag gaagcagaat tggtggaaaa
      901 gaaaggttgg aatgcttttg agcagttctt gctggataat gaagttgaca acctttcgga
      961 tatgaagcgg aaaccgtttg cttggtaagg gctttcttaa atttgattaa aggtcgtctg
     1021 aaattaagct tgtttttcag atggtctttc cttcatttga agcaggatat tgagaactga
     1081 gttcttcaaa aatcctacac ctgctccttc cacggcagca ccttggtcaa aacggcagac
     1141 ggctgaaaag caaacaccgt ccgtcgtgtt gccgtttgcg gatgagtacg ggtcaacccc
     1201 aatgccgccg aaaccgtcga agccgccttc aacattgcca ccgccaaagc cgcgaagttg
     1261 gcaaaaacgg taaaaccggg gagataaaag ccgatggcag gaaagtaaat gtgaggatag
     1321 acagtacgga ggcagacctg ctttatccgg cagggcaata agaaaacaaa acttagatat
     1381 ggaaaacgat tgtgaagatt aaaccattac aattttctaa caataatcac agattttatg
     1441 tggacaaaat ttgtgttacc gaacaagatg tgaacatttt ggcatccgac cgaaattatg
     1501 aattaaatat tgaaatattt attgacaata taattcattt tcaaataacg gatgaatctt
     1561 ataaagtaaa attttcagaa tatttattcg aaaataaaac agaaaataat tcggatggga
     1621 atcctactgc aaattatttt ttcgagataa tagatgacag ttatatggac tggttgaaag
     1681 aagaaagttt tggttttttt gaaaagaaat attataaggc ttatattttc ttttttagcg
     1741 attctgtaat agaagttatc agctcgacag aacctgtatt ttattcaaaa taacaaatta
     1801 tcaaacaaaa ctctgattaa aaacccaaca atcagaatgc cgtctgaagc ggtattcggc
     1861 tttcagacgg cattttctcc gattggcaca ggatgcaggg gcttgccggc ttgattttgc
     1921 cgaagttgtt ggctcgtata gtggattaaa tttaaaccag tacagcgttg cctcgccttg
     1981 ccgtactatc tgtactgtct gcggcttcgt cgccttgtcc tgatttaaat ttaatccact
     2041 ataatttgga agaggcagca acctatggaa aatagatttg aaaaaatcgt taaggatatt
     2101 tttagtttta cccaatttgt ttccgaagcg gaaaataaaa ttcctaacca atcctcccgt
     2161 tattacgact gctggtggga attggaaatc ctgaatgcct ccgcattgga agattgggaa
     2221 atatcgggaa agcctgaaaa atgggagaaa tggacagaaa aatatcagac cgaagcgtta
     2281 cagtcagtta aggatctatt tgccgaagaa gttcctgaaa ataatatagt ttgcgatatt
     2341 tctcattttt tgcattttgc cgatcgggca gaagccgaca ttcccaatca atcacgttta
     2401 tatgccgaat attggtggga aatggaaacg ttcaaaatgt cggttttaaa agattgggag
     2461 cgttcaggca gaccactaga ttgggaaaaa tggcaaaggc aatatcaatc cgaagcattt
     2521 gaattagtca aaaatttgaa ccgaagcaaa caagagtaac aagccgcagc ctgcataggg
     2581 gtttttaaag tacggcagca accgtagatt agattaaaaa tcaaacaatc aaagcccgcc
     2641 tgatataatc gatatatcct gttaaacgaa aaataaatat gtttaaaaaa ttcaaaccgg
     2701 tattgttgtc attttttgca cttgtatttg ccttttggct gggaacgggc attgcctatg
     2761 agattaatcc gcgttggttt ttgagcgata cggcaactga aatacctgaa aatccgaata
     2821 cttttgtggc gaaacttgcc cgcctgttcc gaaatgccga cagggcggtt gtcatcgtga
     2881 aggaatcgat gaggacggag gaaagccttg ccggagctgt ggatgacggt ccgttgcagt
     2941 cggagaagga ttatctcgcg ctcgctgtcc ggctcagtcg tttgaaagaa aaggcgaaat
     3001 ggtttcacgt aacggagcag gaacatgggg aagaggtctg gctggattac tatatcggcg
     3061 agggcggttt ggttgcggtt tcgcttttgc aacgctcgcc ggaagcgttt gttaatgccg
     3121 aatatctgta tcggaacgat cgtccgtttt ctgtaaatgt gtacggcgga acggctcacg
     3181 gggaaaatta tgaaacgaca ggagaatatc gggttgtttg gcaaccggac ggttcggtat
     3241 ttgatgcgtc ggggcgcggg aaaatcgggg aagatgttta tgagcattgc ctcgggtgtt
     3301 atcagatggc ccaggtatat ttggcgaaat accgggatgt cgcgaatgat gagcagaagg
     3361 tttgggactt ccgcgaagag agcaaccgga ttgcatcgga ctcgcgcgat tctgtgtttt
     3421 atcagaatat gcgggaattg atgccccggg ggatgaaggc gaacagtctt gtggtcggct
     3481 atgatgcgga cggtctgccg caaaaagtct attggagttt cgacaatgga aaaaaacgcc
     3541 agagtttcga atattatttg aaaaacggaa atctttttat tgcacaatct tcgacggtag
     3601 cattgaaagc ggatggcgta acggcggata tgcagaccta tcatgcgcaa cagacgtggt
     3661 atttggatgg cgggcggatt atccgcgaag agaaacaggg agacagactg cctgattttc
     3721 ctttgaactt ggaagatttg gaaaaagagg tgagccgtta tgcagaggct gcggcgagac
     3781 gttcgggcgg caggcgcggc ctttctcact gaacgcaaat accgtctgaa accggttttc
     3841 agacggcatt ggagagtcaa acgctttttg caatgccgta tatgccgaac ccggttcgct
     3901 acaaattgaa aaccacaatc caaaaaacgg aaatacacat tatcaaacaa acaaataagt
     3961 tgacatattg tgcgatattg agggctgtac cgtattttcc cgttctttat atgagcggca
     4021 ggcatcctca taaaagaagg gataccgata aggaaaaatc cctcggtata aaaaatatac
     4081 tgattaatca atacgaatcc aacaaacagt tcatcatttt cttttctgtt ggcgtataaa
     4141 aaccgctcgg aaaaaaataa aatatcaaaa cggcaaaata aaaatactga tatatgcctt
     4201 cactttcata gggcgacgct ctatgtgccg tcctgtgtgt tgaaacgtcg gttttcctta
     4261 ccgcctgccc cgcttttccc tgattgctgc caactgccgt ccgaacctga accgtcaggt
     4321 ttcagacggc attttttctc cctgttccca caattgccgt ttttgtgcgg cggtcagttt
     4381 tttgacggcg atttcctgtt tgagcgcagt gcctttatcc atgccgcctg caacgatacg
     4441 catcgccacc ggtttgaata cgcgggtata tttcgcgcct tttccggctg tgtggatggc
     4501 aagccgctgt tgcggattcg ggctgatacc gcaatagaac gcgctgtttt cacacaatat
     4561 caggtaaacg ctccagtttg acgcgttcat ctgcctgcat cctgggtttt ttccccgttc
     4621 caaggcgcaa ctttcagcag cagcagcata cccggcaggg caagtatgaa acacagccgg
     4681 aaaaacggta cataacccat ccattcgatc agatagcctg caaaggaatt gatgaccgtg
     4741 cgcggcacgg cggacaggct ggtaaacagc gcaagctgcg ttgccgtaaa cgcgggattg
     4801 gtctcgcgcg ccatatacga tacaaacgcc gccgtcccca accccacgcc gaccgcttcc
     4861 gcgccgataa ctgccgccag catcagcctc tcgcctgtgc cgaccgtgtc gaaatgtccg
     4921 aaccctgcca gccatacaaa ccccaaaacc gttattgcct gcaccgcgcc gaatagccac
     4981 aaggctttgt ttacgccgat tttcagcatc cacacgccgc ccaagatacc tgccgccact
     5041 gccggccaca gtcctgcatt tttggcaatc aaaccgatgt cggtcttgct gaaacccata
     5101 tccagataaa acggcgttgc caacgcggtt gccatactgt cgccgagttt gtaaaggaag
     5161 ataaacagca gcacgcacac cgccgaagcg atgcccttgc gcgtaaaaaa ttctttaaac
     5221 ggctctacca cggtctgctt caacgtttta ggaacggaag gcggcaacac gggttcgtgc
     5281 gcgagaaaca gcgtcatcag aaggccgggc agcataaata atgaagtgat aacaaatact
     5341 tctgaccacg gcatcctgtc tgccaacacc aaactcaatg aaccgggaat cagggcggca
     5401 acccggtagg cgttcacatg aaccgagttg cccaaaccca attcttcgtc cgacaaaatc
     5461 tcgcgcctga acgcatccaa tacaatgtcc tgactggcgg aaaaaaaagc gacaagcacc
     5521 gacaggccgg caatcaacgg cagatgattc cgggggttta aaaaggcata tgccgccaaa
     5581 gccgccagta accctgcctg cgtcagcagc atccacccgc gccgccgtcc caaaacgggc
     5641 agcctgaccg cgtccatcag cggcgaccac aaaaatttcc aagtaaacgg cagaccgatc
     5701 aacgccatca gcccgatgct cttcaaatcc acctgctcgc tgcgcaacca cgccggaatc
     5761 aggttaatca gaaagtacag cggcagcccc gaggcaaacc ccgtaaaaat acagacgagc
     5821 atattacggg aaaagatttg cccgataaaa cctgatttcg atgcagtcat cgttcgtaca
     5881 gccaaaaaac aaaagcctat tgtagcaaat atcgcccgcc tgctgaaaaa tgccgccacg
     5941 cgccgccggt ttgccgttcc aatgcaaaat gccgtctgaa actgtgttca gacggcattg
     6001 ctactgaaac ccgacggttt acaggctgta atacatttca aattccagcg ggtgcggcgc
     6061 catacggatg cggcggacat cttcctcttt aaaggcgatg tagctgtcga tccagtcttt
     6121 gctgaacacg ccgccgcgca gcaggaattc gtggtcggcc ttgagggcgg caagtgcttc
     6181 ttccaaagaa gcgcagacgg tcgggacgag cgcgtcttct tccggcggca ggtcgtacag
     6241 gtttttatcg gcagggtcgc ccggatggat tttgttttga atgccgtcca aaccggccat
     6301 cagcagggcg gcaaatgcca aatacgggtt ggcggtcggg tcggggaaac gcgcttcgat
     6361 gcggcgcgcc ttgctgctgt tcacagacgg gatacggatg gaagcggaac ggtttttggc
     6421 ggaataggcc aatttggtcg gtgcttcaaa gtgcggcacg aggcgtttgt aggagttggt
     6481 ggacggattg gtaatcgcgt tcagggcttt ggcgtgtttg atgatgccgc cgatgtagta
     6541 gagcgcggta tcggacaaac cggcatagcc gtcgcctgcg aacaggtttt gaccgtcttt
     6601 ccaaatggat tggtggacgt gcataccgct gccgttgtcg cccataatcg gtttgggcat
     6661 aaaggtggcg gttttgccga aattgtgggc aacgttttgg atgacgtatt tcatatcttg
     6721 ggtttggtcg gcgcgtttga ccaaagtggc gaaacgggtg ccgatttcca tttggctgcc
     6781 ggtaccgact tcgctgtggt ggacttcgac ttcgatgccg agtccttcca aaatgttcac
     6841 catggcggag cgcaaatctt gaccgcagtc aatcggcgcg acgggcgcgt agccgccttt
     6901 gacggcgggg cggtggccgg tgttttgacc gtccatatgc aggccgcttg cccacgcgcc
     6961 gctttcggac gtgatttcgt aacgggtttt gtgcatgtcg gtttcaaatt ctacgccgtc
     7021 gaagacgaag aattcgggtt cggggccgaa ataggcggtg tcgccgatac cggaagattt
     7081 caaataggct tcggcgcggc gtgcgatgga gcgcgggtcg cggtcgtaac cctgaccgtc
     7141 ggcagggtcg atgacgtcgc aggtaatgac gacggtaaca tcatcataaa aaggatcgac
     7201 gaaggctgtg gacgcatcgg gacgcagctg catatcggaa gcctcaatgc ctttccagcc
     7261 gccgatggac gagccgtcaa acgccggtcc gttttcaaac cactcttcgg ggtcttccaa
     7321 cacgatgcgc gcaggcacgg taaagtggtg ctgcttgcct ttggtatcgg tgaagcgcaa
     7381 atcgacaaag cgggcttcgc tttcttcaat caattttacg gcgtttttaa tggacatctt
     7441 cagctcctga aaaagggaaa tggcaaaata tgcggaacgc ggcggcttga cgcaaagtcg
     7501 gcacgatttt accacgccga tgccgtctga aaccggtttt gcgtcaaatt ccaagccgga
     7561 aaatatagtt tcaaacggca agattgtcaa caatatgctt cggtttgtct gttcaaaagc
     7621 ggaatgcccg cccttttgtc cgaatgccga caaaacagat gccgtctgaa ggcggtttca
     7681 gacggcatcg ttcccatctt cgcgccgccg ttataaaatg accgccccgc ctttatggaa
     7741 tatgactatg cacgccctcc cccgctacgc cgttttcggc aaccccgccg cccacagcaa
     7801 atcgccgcag attcatcagc aatttgccct tcaggaaggc gttgacatcg aatacggacg
     7861 catttgcgcc gacatcggcg gtttcgcgca ggcggtttcg acattttttg aaacaggcgg
     7921 ttgcggggca aacgttaccg taccgttcaa acaggaagcg tttcatctgg cggacgaaca
     7981 ttccgaccgc gctttggctg ccggtgcggt caatacgctg gtgtggttgg aagacggcag
     8041 aatacgcggc gacaataccg acggtatcgg tttggccaac gacatcacgc aggtcaaaaa
     8101 cattgccatc gagggcaaaa ccatcttgct tttgggcgcg ggcggcgcgg tgcgcggcgt
     8161 gattccggtt ttgaaagaac accgccctgc ccgtatcgtc attgccaacc gtacccgcgc
     8221 caaagccgag gaattggcgc ggcttttcgg cattgaagcc gtcccgatgg cggatgtgaa
     8281 cggcggtttc gatatcatca tcaacggcac gtccggcggc ttgagcggtc agcttcctgc
     8341 cgtcagccct aaaattttcc gcgactgccg ccttgcctac gatatggttt acggcgaagc
     8401 ggcaaaaccg tttttagatt ttgcacgaca atcgggtgcg aaaaaaactg ccgacggact
     8461 gggtatgcta gtcggtcagg cggcggcttc ctacgccctc tggcgcggtt tcaaaccgga
     8521 catccgcccc gtcatcgaac acatgaaagc cctgtaacca tgttccgcat cgtcaaatgg
     8581 ctgattgccc tgcccgtcgg catctttatc ttcttcaatg cctatgtgta cggcaacatc
     8641 attacctacc gcgccgtcgc gccccatcgg actgccttta tgtcgatgcg gatgaagcag
     8701 tttgaacaag aaggtcgcga tgtcgcactg gattaccgct ggatgcccta caaacgcatt
     8761 tccaccaacc tgaaaaaagc cctgattgct tccgaagatg tccgttttgc cggacacggc
     8821 ggcttcgatt ggggcggcat tcaaaacgcc atcaggcgca accggaacag cggcgaagtg
     8881 aaggcgggcg gatcgaccat cagccagcag cttgccaaaa acctcttcct caacgaaagc
     8941 cgcaactatc tgcgcaaagg ggaagaggcg gccattacgg caatgatgga agctgttacc
     9001 gacaaaaaca ggattttcga actgtattta aactcaatcg aatggcacta cggcgttttc
     9061 ggcgcggaag ctgcgtcccg gtatttttat aaaaaaccgg ccgcagacct gaccaaacag
     9121 caggcggcga aactgacggc actcgtcccc gccccgctct actacgctga ccatccgaaa
     9181 agcaaacggc tgcgcaacaa aaccaatatc gtgctcagac gcatgggttc ggcagaatta
     9241 cccgaaagcg atacggactg attgtttcag atatggaaat gccgtctgaa ctgcggttcg
     9301 gacggcatat gttttctggt tcttatagtg gattaaattt aaaccggtac ggcgttaccc
     9361 cgccttgccg tactatgttg tactgtctgc ggcttcgtcg ctttgtcctg atttttgtta
     9421 atccactaac ctgcccgaca gccgcaaaga ccatgtcgtg catggcaggt atttgcggtg
     9481 cgggactgct gccgaaaacg gcaggcaaaa aataaccgct tataagcggt tttttggtgg
     9541 ccagaggcgg aatcgaaccg ccgacacacg gattttcaat ccgttgctct accaactgag
     9601 ctatctggcc tgtatctgtc tcgttaagag gtgcgtatta aaccaaattt cgggacgaca
     9661 cgcaagcgga tttgtcgata ttttttaccc aaccgactaa gtcgatgttt taagtttaaa
     9721 ttaatttcac agaaaaatag tgcaaatcgg ttttttgccg ttcgttcggc gatgttctgc
     9781 tttgcagacg gcaaaacgct tcaatatttg aagttctcgc ccaaatagac ggcgcgcacc
     9841 tgttcgttgc cgaccaaatc gtcgggcttg ccggaagcca gcaccgcacc gtctgaaatg
     9901 atgtaggcgc ggtcgcagat gctgagggtc tcgcggacgt tgtggtcggt aatcaatacg
     9961 ccgatgccgc gcgatttgag gaaaccgatg attttctgga tgtcgatgac ggcgatggga
    10021 tcgacgccgg caaaaggttc gtccaaaagg atgaaacgtg gtttcatggc gaggacacgg
    10081 gcgatttcga cgcgccgccg ttcgccgccg gacagcgacg gcgcaggatt gcggcgcaag
    10141 tgtccgatat tgaggtcggc gagcagtttt tcgatttctc tgtcgatttg atttttatct
    10201 ttggttctga tttccaagat ggcgcggatg ttttgttcga cggtcatttt gcggaatatc
    10261 gaggcttcct gcggcaggta gccgacaccc aggcgggcgc gttcgtgtat gggcaggtgg
    10321 cgcaattccc gcccgtccag catcacgctg cctgcgtcgg cagcgatgag cccgactatc
    10381 atgtagaagc cggtggtttt gcctgcgccg ttgggcccga gcagtccgat gacttcgccg
    10441 ctttcgatct caagggagaa acttttgacg acttggcgtt ttttgaaact tttctgcagg
    10501 ttttggacga cgaggcggct gttgtttgcg ctcataaatc tcttctcggc atttcggtcg
    10561 gcaaatgccg tctgaactgc gtttccgttt cagacggcat cggggttatt cggttttttg
    10621 tgtgcttgaa ggctggatga cgacgctgac cctgccggtt ttggaagcgg atttcgcacc
    10681 cgatttcgtg ctgccgttga tggtatagac ttcggttttg gtgttgtagg taatgaccgc
    10741 accttcggca acgtcgccgc cgcgctgcac tttggcattg ccggtcagaa cgacggtgct
    10801 tcctgcggag gaataggtaa cgttgtttgc ctgaccgcgc accgtccctt tgcccccgtc
    10861 caacgtttgg ctgaagcgga cgggcgaacc ttccgccctc acggattcgc cgcctttgcc
    10921 gccgcgtgtg acgttgacgc gcgaggcgga aatgttgagc gtaccctgtc tgatgatgac
    10981 attgccgcta aatgtggtac tttggttggc ttgatcgagc gaaccttggt cggcttcgat
    11041 ttggatgggc cgtctgctgt cgctttgaag ggcaaaagcg ggagaagttg caaaaattac
    11101 aattaaaaca aatagcttac atatcttttg tatcataaat cgcggctttc actttagatg
    11161 agaagttcaa catgcctgtt ttgtggttgt aggtcatacc gcccgcctga ccgtgcgacg
    11221 cgccatattg gaaactgaca ggcgtatcgg tttgggcata ttgagattcg gtatcgacgt
    11281 gcagtttttc ggtttcgact ttacccgcct gccgcctgcc gtcggcggtt ttggtcagca
    11341 caacgttgtt tttaaaaaga acctgtttgt tttcggtatg gtaaacggct tcatcgctgc
    11401 cgacttcgta caacagcctg ccttcttgga agaacacgag atgcggcgaa tcaaaatgga
    11461 tgtcgctgtt ttcgggaaac tgtttcgcac ctttcgcgct caaatgttct ttcaagtatc
    11521 cctgttcgtc aaaccgcctt ccgtccaagc cgtccattgt gtattgaggt tcgtcgggat
    11581 tgagcctgac ttcctcgatt tcgacttcgc tgatacggcc caaccatgcc gacaggccgc
    11641 ccaaggcaac cgccaatatc aatgggaacg caattccgta ccgccatctt actttcattt
    11701 gatgtacccg ttcaaagccg cgcccaacgt acctttcgcg cggataatca ggtcacacac
    11761 ttcgcgcacc gcgcccgcac cgccggggcg tttggcaata taggcggcgt gttgcagcgt
    11821 aaaccaatgc gcgtcgggga cggcaaccgg caatccgcag cgcaccatca ccggcaaatc
    11881 gaccacgtcg tcgccgacaa aggcgcactc ggcttcttcc aagcctgcct gcgcgcgcag
    11941 ctcttcatac gcggcgcgtt tgtccgaaat gcctttgaag tagtaattta tgcccaactg
    12001 tttgacgcgg atgccgacgg agggcgcgtc ccgacctgtg ataatcgcag tctgcacgcc
    12061 gcttgcctga agcattttca ggccgtgtcc gtccagtgtg tgaaacgatt tgatttcttc
    12121 gccgtcgtcg cggataaaga tgcgcccgtc ggtcaaaacg ccgtccacat ccaatatcag
    12181 cagtttgatt ttggcggcgc gtgcctgtaa ttcgggagaa attgcctgca ttgggatttc
    12241 cttgttttca gacggcatat cttaccacaa atgccgtctg aaacggctgc aacgccttat
    12301 acaatccgcg ccgccaaaag gtcgtgcata ttcagcgcac ctgtcagcac gccgtctgca
    12361 tcggtaacca gaagcccgtt cacatggttt gcctgcatga ctttcagggc ttcggtggcg
    12421 aggcgttcgg cggagatggt tttagggtgt gtatgcatga cttcgtctat cgaaagaccg
    12481 gtaaaattgt cgcattcttg aaacaggcgg cgcaaatcgc cgtcagtaaa cacgcctttc
    12541 agacagcctt gcccgtccgt taccgccaac atgcccaaac ctttctcgct catgctgacg
    12601 atggctcctt tcaagggcgt gccgagtcgg acggcgggca ggccgccgcc tttgtgcata
    12661 atgtcggcaa cgcgcaaaag caggcgtttg ccgaggctgc cggcagggtg gatcaaggcg
    12721 aagtcgtcgg gcgtgaacgc gcgggcgcgc agcaggacga ccgccaacgc gtcgcccaaa
    12781 gccataacgg cggtggtgct ggtggtcggg gcaagcccca acgggcaggc ttcttgcgaa
    12841 accgatgcgg tgatgtggat atcggcatgg cgcgccatgg ttgaatcggg gcgggcggtg
    12901 atgcagacga gggtaatgtc tttgcgtttg agcgcgggga tgatggcggt gatttcgtcg
    12961 ctttcgccgg aattggaaat ggcggcgacg acgtcgttgt ccacaatcat gcccaaatcg
    13021 ccgtgtgccg cttccgcagg gtggacgaaa aacgcgggcg tgccggtcga ggccatggtt
    13081 gccgccattt tgcgcccgat atgtccggac ttgcccatac ccgtgatgac gaccctgccc
    13141 ttgcagtgca acaacgcgtc tgccgcaagg acgaagtttt cgtccaattc cgccgcaatt
    13201 tcgcgcaagc cttccgcttc ggtgtgcaac acttcgcgcg cccagtcgag atatttttcg
    13261 ttttctgcca ttgccatccg cctttgctgt aacaaaacta cccaaaattt ccgaatcggg
    13321 gtagcataag ccttcaaacc ccgcccatcc atccaaagga atcaaaaatg actattttat
    13381 cggacgttaa agcattagga caacaaatct ggctggacaa cctttcccgc tcgctcgtgc
    13441 aaagcggcga attggcgcaa atgctcaaac aaggcgtgtg cggcgtaact tccaatcccg
    13501 ccattttcca aaaagccttc gccggcgacg cgctttatgc cgacgaggtc gccgccctca
    13561 agcggcaaaa cctcagcccc aaacaacgct acgaaaccat ggcggttgcc gacgtacggg
    13621 cagcctgcga cgtttgcctt gccgaacacg aatccaccgg cggcaaaacc ggtttcgtca
    13681 gtctcgaagt ttctcccgaa cttgccaaag acgctcaagg cacggtagaa gaagcccgcc
    13741 gcctccatgc cgccatcgcg cgtaaaaacg ccatgattaa agtgcctgcc accgacgcag
    13801 gcatcgatgc gctcgaaacc ctcgtttcag acggcatcag cgtgaacctg accctgctgt
    13861 tctcacgcgc ccaaaccctc aaagcctacg ccgcctacgc gcgcggcatt gccaaacgct
    13921 tggcagccgg acaaagcgtt gcccatatcc aagttgtcgc cagcttcttc atctcgcgcg
    13981 tggacagtgc gctggatgca acgctgcccg accggctcaa aggcaaaacc gccatcgccc
    14041 ttgccaaagc cgcctatcaa gattgggaac aatatttcac cgcccccgaa tttgccgcac
    14101 tggaagccca aggcgcaaac cgcgtgcagc ttttatgggc atctaccggc gtgaaaaacc
    14161 cagcctatcc cgacacgctc tacgttgaca gcctgatcgg cgtgcacacc gtcaacaccg
    14221 tccccgatgc cacgctcaaa gcctttatcg accacggcac ggcgaaagcg acgctgaccg
    14281 aaagcgcgga cgaagcacgg gcgcggctcg ccgaaattgc cgcgctcggc atcgatgtcg
    14341 aaaccttggc ggcgcgtttg caggaagacg gtttgaaaca gtttgaagaa gcctttgaaa
    14401 aactgctcgc gcctttggtt taggcatttc cccgatgccg tctgaaaggc tttcagacgg
    14461 catcgccctt ttgaccggac acagtgcggc atatcaagga tccgccctaa aaatattaat
    14521 gggcaagaag ttaatgcacg acattacccg ccaacacaaa aggaaataga atcatggcaa
    14581 aagcactcga aatcatttca cccgatgaaa tttattcaga tctgattttt aaggatccgg
    14641 tacctcccca tactatatat acagaattga tgaaactggt tggtagaaat gcaggcaatg
    14701 agagaattgc agatagagcc ttcgattttt tctcgcctgc tcttatggat gattcagcta
    14761 ccgaagagca atataatgcc ctatatgatt taaccttgct tgaagagccc gggatggaac
    14821 tggataaaga tgagataacg gctttgatta atagtcttaa ataaaggggc tgtaccggat
    14881 atgtacacag gacgcttcgc ccccagcccg accgggttgc tccacatcgg ctctcttctg
    14941 accgccgtcg cttcctatgc cgatgcacgt tcaaacggcg gcaaatggct ggtccgtatg
    15001 gaagacctcg atccgccgcg cgaaatgccg ggggcggcaa gccatatcct gcacacgctc
    15061 gaggcattcg gattcgagtg ggacggcgaa gtcacctatc agagccgccg ttacgccctg
    15121 tatgaagaaa ccctataccg tctgaaaacc gccggactgg tctatccctg ccattgcaac
    15181 cgcaaagact ggcaggccgg ggcaaggcgg ggcacagacg ggttcgtcta taacggacgc
    15241 tgccgccacc ccggccaacg ccctgcactg caaggcaaac agccgtcgtg gcgcatccgc
    15301 gtccccgacc gcgacatcgg cttttcagac ggcatcgtcg gcagttacac ccaaaacctc
    15361 gcccgcgaca tcggcgattt cgtcctgttc cgtgcagacg gttactgggc ataccagctc
    15421 gccgtcgttg ccgacgatgc cgaacagggc gttacccaca tcgtgcgcgg acaagacctg
    15481 ctcgtttcca caccgcgcca aatctatttg cagcagtgtt tgggcgttcc gacaccgcaa
    15541 tatgcccacc tgccgctgct gaccaacgcg caagggcaga aatggtcgaa gcagacgctc
    15601 gcccccgcat tggattcaaa ccgccgcgaa caacttctcc gccaagtgtt ccgctacctc
    15661 aagctgcccg aagcaccgga aaccgaccgc cctgccgaat tgctcgactg ggcggtggca
    15721 cactgggata tggacaaagt gccgaaacac gccattaccg ccccctaaac accacccgac
    15781 aaacgcacaa atgccgtctg aaaccttcag acggcatttc acacatacag ccccgcccta
    15841 ttcgcgcgta tttgcccgtt cgacttcttt ccacgcttcg aaaatcaggc cgccgtcgtt
    15901 gtctttcaag agcgttgcat cggaaagcat acgccgccaa gtccgcgcgc ctttcaaacc
    15961 gtgcatcagc ccgaggctgt ggcggacgat gtggcgcagg attgtgccgc gtccggcttg
    16021 gatttggact tggctgtatg tgtagaggcg ctgcaccaaa tcggcgtatt cgatcggggc
    16081 gcggttgtca ccgtaaaaca gcctgtccca ttcgcgcatc accatcgggt tgtggtatgc
    16141 ctcgcgcccg accatcacgc cgtcaacgtg ttgcaagtgt ccggcgattg cttcgttggt
    16201 ggtgatgccg ccgttgatga tgatttccag ctcgggaaac tcctgcttga ggcggtaaac
    16261 gtaatcgtat ttcaagggcg gaacatcgcg gttttctttg ggcgaaagac cgtccagcca
    16321 agcgttgcgg gcgtggacga tgaaggtttt gcaggcggtt ttgtcgcgca gcgtgccgac
    16381 gaaatcggca acggtttggt attcggtctg cctgtccaca ccgatgcggt gtttgacggt
    16441 aacggggatg ccgaccgcgt cgtgcatggc gttgaggcag tcggcaacca gcccgacttc
    16501 gttcatcaga cacgcgccga acgcgccttt ctgcacgcgc ggactgggac agccgcagtt
    16561 gaggttgacc tcgttgtagc cgtattcttc ggcggctttg gcggctttcg ccaaatcgga
    16621 cggatcgctg ccgcccagtt gcagggcgac gggctgctcg ccttcgttaa acatcaaaaa
    16681 acggtctttg tcgccgtaaa caatcgcgcc ggcgttgacc atttcgctgt aaagccaagc
    16741 atttcgggta atctggcggg caaggtaacg gtagtgcctg tccgtccagt cgagcatcgg
    16801 ggcgacgcat aaacgccttt tccctttatg ttcaataagt tccttgttta tcataaggtt
    16861 ttcacattct tttaactcaa acattcttta ttatttgctt ggatttttgc ccatttcact
    16921 atatttgacg taccatactt gcaccataat ttttaattct tggaatggtg caaatggcaa
    16981 caataaccaa gcggcgcaat ccttccggcg aaacagtata tcgggttcag gtacgggtcg
    17041 gcaagaaagg ctatcctgct ttcaatgaga gcaggacgtt cagtaaaaag gctttggcgg
    17101 tcgaatgggg gaagaaacgg gaggcggaaa tcgaagccgg cccagaactg cttttcaagc
    17161 gcggcaaggt caagatgatg acgctgtccg aagctatgcg gaagtatctt aacgaaacgc
    17221 ttggagcggg tcggtcaaag aaaatgggct tgcgttttct gatggagttc ccgattggcg
    17281 gcatcggcat cgataagctg aaacggtctg atttcgcgga acacgttatg cagaggcggc
    17341 gcggaatccc cgaactggac attgcgccaa tcgcggcttc tacggcattg caggagctgc
    17401 aatatatccg ttccgtgcta aaacacgcct tttatgtgtg ggggcttgaa ataggctggc
    17461 aggaattgga tttcgcggca aacgggctga aacgctcgaa tatggttgcg aaatctgcaa
    17521 tcagggacag attgccgacc acggaagaac tgcaaaccct gacaacttat ttcctgcggc
    17581 aatggcaaag caggaaatct tccataccga tgcacctgat tatgtggctg gcgatttata
    17641 cgtcaaggcg gcaggatgag atttgccgcc tgctgtttga cgactggcac aaaaatgatt
    17701 gtacccggcc ggttcgtgat ttaaaaaatc cgaacggcag cacagggaat aataaggagt
    17761 ttgatattct gcctatggct ttgccggtca ttgacgagct gccggaggaa tcggtcagga
    17821 agcgtatgct tgccaacaaa ggcatcgccg acagccttgt accgtgcaac ggaaaatcgg
    17881 tttccgccgc ttggacgagg gcgtgcaagg ttcttagaat taaagacttg cgcttccacg
    17941 atttacggca cgaggctgct acccgtatgg ctgaagacgg cttcacgatt ccgcaaatgc
    18001 aacgggtaac gctgcatgac ggttggaaca gcctgcagag gtatgtgagc gtacgcaaac
    18061 gctcgacgcg gctggatttt aaagaggcaa tgatgcaggc gcaaagcgat ataaaatccg
    18121 ggaagtgatg ttaaattaaa ggggatgtgc cgcatcccct gattcctccg tcttgcatca
    18181 agaccggaat ttccgatatt ccgattttgc cttttcttcc ttgtctgcga ttgcctttgc
    18241 aagctctcgg acgctgacga aatattccga ttttcggctg tcggacaatc ggaaaatcgg
    18301 aaacggaagc tcgcaccgta cccccgatgc tttaaacgcg ttaaagccga tatgggcaaa
    18361 aaagtctttg tgtacttccc tcggcgggat atacgggcgg ccgccgtatg cgattaaaag
    18421 cccttgttct ttactgatgt tcatatatac ctctttctgt taattctgct ttgtccgggt
    18481 ttccgctttt ttacggcttg gcggatgttc agcctgcgtc ggtgcataag tcctcctgtt
    18541 cgggcggttg ttcagccggc cgcccctccg aaaagtggca ggctgcacgg gaagtagtgt
    18601 ttttttccgt atttctctgc ctgtatcagg ttgcacaggc ggtcgcaaaa gccgtcaaat
    18661 tcggcatgcg gccagccttc tatctcgttg accattttgc tgaacctcaa ttcccgggca
    18721 atccgctttg ccgctgcgtc acggcattcg tgttccaaga ggacggggtc ttccttttcc
    18781 ttcggcggtg caggttcttg cacggtttgc cacaagtccg gttcgatttg ccaagtgtcg
    18841 gcggcaagca gggtgtctgt tttgcagtcg tacagttcgc ggatgcagcc gccgtcggtt
    18901 ttgtctttgt cgataaccag taaaagcact tcgatcggcg tgtcttcaaa cgcgttttgt
    18961 atgcggttga gttctgccag ccggttgccg atgatttcgc ggaatcgctg ctcgcttttg
    19021 cggtaggcga tgccggggaa caggatgaag aatccgaagc ggcgggcgtt ttccaatcct
    19081 tttaaaacga agatttcatc ggctacgccc gatttcttcc acgggtattc ctgtgcgata
    19141 cggctttttt cgtcttcgct caaatccttt agcttgatgg aaaacggcgg attcatgacg
    19201 gtgcagtctt gcggttcgcc gtcggtataa aggaaaaggc ttgtgttgta tactttggcg
    19261 gcagggtagt tttgcaacaa ggctttacac gcttccgcct gtatctctac ggcgcggaaa
    19321 tcggacggtt cgatgtactg ctccagctgc ccgcttcctg ccgcgccgtc aaatacgccc
    19381 gggtgttcgc cgcagtatcg gcggactttg gcggcaacca gccgcctcaa gctgccgccc
    19441 gtgatgtatt cggcgtagcc gccggctttt ttacggttgt tgtgtccttg aaacgtcatg
    19501 ggttttcgtc cgttctctgg cttgtccggg tgtggcggag cgtttgcgga tgcggctgac
    19561 gcgctgcggt ttgccgtctt cgccgcgctg aaaaacgctt gttccgccgc gccggcagcc
    19621 gtgccgctta tgcctgatgc cgtgtttgaa gccttttgtg ccggaatcgt gatacacgca
    19681 ggtccggatg tgcgtcctca tgccgccgct tccctgtgca tgaggctgat caggtttgcc
    19741 gtccgtttga aatgttcggt ccagttgaag cagctgaagc cggcgccgtt gtcgcaatgc
    19801 cggatgacgt cttccgagtc ttttacggcc cggaccaacg cgccgccttt gtccgcaaca
    19861 agcattttca tcatcggccg gcgcacgtcg cttatccgtc gggggcgcac gggattcagc
    19921 ggcttgccgt acatcgccga aagctccttg tccgcctgaa tgcccacatc gtaaagcagg
    19981 ttcgcggcgt gtaggatccg gcctgtaaat tccgcgtacg gcattttcag gcggcgggct
    20041 tccgcgcgcg aatccgcgcc gttgacgact ttgccgaccg ccgaaatcaa accgccgtca
    20101 agttctaaaa acgccatggc tttcttggca acccgcacgg ccaatgagta tgccgatact
    20161 tcggctatca gccgtgcagg cgcgtctttc agaaattctt cgtacgcggc gaagagattt
    20221 gacagcggac gcagcacgag ttggcgctga ttcgggctaa ggtcgtcgaa atccttagtc
    20281 cattgcgcga ccgcctgcga tgcttcgcgg caggcaaaca gtacgctttc ctcgttcgcg
    20341 gggtcgtccg tgttgcagta caggcccaag cgttgcacct gtttgacgat atgttcggcg
    20401 aagttgatta actcctgatt gcaggcatag cgcatatcct gcaggg
//
LOCUS       DAWTQS010000035        20238 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.035, whole genome shotgun sequence.
ACCESSION   DAWTQS010000035 DAWTQS010000000
VERSION     DAWTQS010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20238
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.035"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            19..665
                     /locus_tag="WNA25_001252"
                     /pseudo
     CDS             19..665
                     /locus_tag="WNA25_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750392.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            complement(737..2482)
                     /gene="recD"
                     /locus_tag="WNA25_001253"
     CDS             complement(737..2482)
                     /gene="recD"
                     /locus_tag="WNA25_001253"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFA9824002.1"
                     /translation="MELQTDEFAQAAARAAIRFLERYAGSGNEVLANCTERLFQALQN
                     GHSFIRLSGDEADALSALAPVVGTSAAPLILEGRRLFLGRMWQLEYDLAAEIKRLAAA
                     GTSAPDAAGARQNLAKWFQGAGSEGQRDAAALALLQFFMVITGGPGTGKTTTVAKLLA
                     LICGENENLPHIALAAPTGKAAAHMARALHRAINGFDAPEAVRRHLLKLEGQTVHRLL
                     KLSPPKMQAAFDHIRPLPFDVLIVDEASMLDTALMLQLLKAVKTGARVILLGDENQLP
                     SVGIGAVLSVLSQKTVLDGETHQRLAGFLPEHGFSVSANPPVLAQNTAHLSFSHRFGD
                     NSGIGCLARAAVSGDEGAWALFDRFPDELEHSECSPNARVERLYRAHKAYWQAVKDGN
                     IEAAYAGISDIVVLAAWRQDAEDFNEAYCSYVRRKMNIPEHLAYFAGEPIMIRQNDYA
                     LELFNGDIGLIMEDVGRQGSLAAYFADADGFKKVAVSCLPEFEPAFAMTVHKSQGSEY
                     REVWLLPPSDAPSDEGDDALSGLSKELLYTAITRAREKFVFFGGKKTFCQAVNTVKVR
                     QTALGSMLERVFSQE"
     gene            complement(2549..3244)
                     /gene="lolD"
                     /locus_tag="WNA25_001254"
     CDS             complement(2549..3244)
                     /gene="lolD"
                     /locus_tag="WNA25_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFA9824003.1"
                     /translation="MSDLILKCEGVGKRYRDGGLDVPVLYGLDLEIRTGESTGIIGSS
                     GSGKSTLLHILGGLDMPSEGRVLLMGEDLRALNQRRLGDLRNRHLGFVYQFHHLLPEF
                     SALENVMMPLLIGKKSRKEAVETAMAMLDKVGLKHRSTHRAGELSGGERQRAAIARAL
                     VTQPKCLLADEPTGNLDRANARNVLDMMLELKTELGTGLVVVTHDDELAGRFERVMVM
                     KDGSLHPRQGANA"
     gene            complement(3237..4484)
                     /locus_tag="WNA25_001255"
     CDS             complement(3237..4484)
                     /locus_tag="WNA25_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HFA9824004.1"
                     /translation="MFSLEAWIGLRYLRAKKRNGFMSFITMVSIAGIALGVTALIVVL
                     SVMNGFQKEIRGQLLNVAPHAEIGYIDNTDTDWRNLLRFAENRKGILAAAPYVSNQAL
                     LANAGEIRGVQIRGILPSEERKVVEYGDKMPAGKFEDLIPGEFDIILGVGLAEALGAE
                     VGGKVTVITPEGNVTPAGVVPRLKQFTVVGLVKTGVYEVDNSLAMTHIQDARVLYRLD
                     KEVAGLRLKLADPQNAPALTATLIPEAQRDTVWVRDWTYSNRSYFEAVELEKRMMFII
                     LTLIIAVAAFNLVSSLVMAVTEKQADIAILRTLGLSPAGVMKIFMVQGAFSGFFGTLA
                     GVVCGVLWGWNVGRVVAFFENLLGVHLINSQVYFIDYLPSDVDMGDVALIACISLGLS
                     FVATLYPSWRASKTQPAEALRYE"
     gene            4710..4988
                     /locus_tag="WNA25_001256"
     CDS             4710..4988
                     /locus_tag="WNA25_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824005.1"
                     /translation="MNIRKISALCAVAVFTVSTAYAKEIKIDANNTPYSEADAQKLAA
                     TAVGMGVKEPVSLNGGSGSITVSGSSAAQCVFTVGSGGTLQIQGLNCK"
     gene            complement(5044..5661)
                     /gene="recR"
                     /locus_tag="WNA25_001257"
     CDS             complement(5044..5661)
                     /gene="recR"
                     /locus_tag="WNA25_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HFA9824006.1"
                     /translation="MNSKKQDAFQRLIGALKVLPNVGPKSAQRMAYHLLQQKRKEAEE
                     LVDALQTALRQVRHCARCNTFCEGGLCDICADETRDGRRLMVVHMPADVSNIEAANCH
                     DGLYFVLMGQINTALGMDVSAIALDRLAQRLDGGEIEEIIIATAFTAEGNATAYVLSE
                     FFKNLPYKVSRLSQGIPLGGELEYVDAGTLAQAVYERRLIKEGGA"
     gene            complement(5728..7266)
                     /locus_tag="WNA25_001258"
     CDS             complement(5728..7266)
                     /locus_tag="WNA25_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HFA9824007.1"
                     /translation="MFHSIEKYRTPAQVLLGLIALTFVGFGVSTVSHPGADYIVQVGD
                     EKISEHSINNAMQNEQADGGSPWRDAVFQSLLQRAYLKQGAKLMGISVSSEQIKQMIV
                     DDPNFHDANGKFSHALLSQYLSQRHMSEDQFVEEIRDQFALQNLVSLVQNGVLVGDAQ
                     AEQLIRLTQVNRTIRSHTFNPDEFIAQVKASEADLQKFYNANKKDYLLPQAVKLEYVA
                     LNLKDFADKQTVSETEVKNAFEERVARLPAHEAKPSFEQEKAAVENELKMKKAVADFN
                     KAKEKLGDDAFNHPSSLAEAAKNSGLKVETQETWLSRQDAQMSGMPENLINAVFSDDV
                     LKKKHNSEVLTINSETAWVVRAKEVREEKNLLFEEAKDAVRQAYIRTEAAKLAENKAK
                     EVLTQLNGGKAVDVKWSEVSVLGAQQARQSMPPEAYAELLKAKPANGKPAYVRLTGLP
                     APVIVEVQAVTPPEDIAAQLPPAKQALAQQQSANTFDLLIRYFNGKIKQTKGAQSVDN
                     GDGQ"
     gene            complement(7315..7689)
                     /locus_tag="WNA25_001259"
     CDS             complement(7315..7689)
                     /locus_tag="WNA25_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HFA9824008.1"
                     /translation="MIILHGIPNCDTVKKAKNRLAGYGLEFGFRDFKKQMPSEAEICS
                     WLEQVPLATLFNKRGTSWRKLDAETQQKALSSTAEAVKLMSEMPSLIKRPVLECGSRV
                     YVGFSEETYDGIFNRQAPCRQG"
     gene            7839..9467
                     /locus_tag="WNA25_001260"
     CDS             7839..9467
                     /locus_tag="WNA25_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFA9824009.1"
                     /translation="MISTNGITMQFGAKPLFENVSVKFGEGNRYGLIGANGSGKSTFM
                     KILGGDLEQTAGEVAIENGVRLGKLRQDQFAYEDMRVLDVVMMGHTEMWAAMTERDAI
                     YANPEATEDDYMKAAELEAKFAEYDGYTAEARAAELLSGVGISEDLHNAKMAEVAPGF
                     KLRVLLAQALFSKPDVLLLDEPTNNLDINTIRWLEGVLNQYDSTMIIISHDRHFLNEV
                     CTHMADLDYNTITIYPGNYDDYMLASAQSRERALKDNAKAKEKLQELQEFVARFSANK
                     SKARQATSRLKQADKIKSEMVEVKPSTRQNPYIRFEADEKAKLHRQAVEVEKLAKRFE
                     TQLFRNLNFILEAGQRLAIIGPNGAGKSTLLKLLAGAYNPEYSDGLLPDEGSIKWAEK
                     ASVGYYPQDHENDFDVDMDLSEWMRQWGREGDDEQVIRGTLGRLLFGSNDVVKKVKVL
                     SGGEKGRMLYGKLLLLKPNVLVMDEPTNHMDMESIESLNMALDKYNGTLIFVSHDRQF
                     VSSLATQIIELDGKGGYEHYLGDYESYLEKKGVA"
     gene            9546..10778
                     /locus_tag="WNA25_001261"
     CDS             9546..10778
                     /locus_tag="WNA25_001261"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HFA9824010.1"
                     /translation="MQTYLVGGAVRDYLLGLPVKDRDWVVVGADAQTMLAQGFQPVGK
                     DFPVFLHPKTHEEYALARTERKTAKGYAGFSFHADKDVTLEQDLMRRDLTINAMAQDA
                     DGKIIDPFGGQRDLAAGILRHVSPAFAEDPVRILRAARFAARYGFEIAEETIKLMRQM
                     VENGEADALVAERVWQELAKGLMEKNPRKMIEMLRECGALQVLLPEVDALFGVPQRAD
                     YHPEIDSGIHTLMTLQRAADMGLSLPERYAALLHDLGKAKTPPDILPRHHGHDINGVE
                     PVREVNQRLRAPRQCAELAELVCRWHIIFHQVGQLKSQTILNVLKKTDAFRRPERFQT
                     ALNVCIADTQGRLNREHTPYPQRAHWLALLEAANQADSGKIAAECRAQGKAHFIAEQI
                     DRARLAQIAPLQKAFRGA"
     gene            11197..11505
                     /locus_tag="WNA25_001262"
     CDS             11197..11505
                     /locus_tag="WNA25_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824011.1"
                     /translation="MTLKTDLLPKINNEDYQRLILKHSAEFSGGETRLLNEILEKFNF
                     DVVQAQALAQAVMQQVRFDPNAYHIDSDDEDTTGICPHCINPPMPPLRDYLVWRETRG
                     "
     gene            11587..12618
                     /gene="ruvB"
                     /locus_tag="WNA25_001263"
     CDS             11587..12618
                     /gene="ruvB"
                     /locus_tag="WNA25_001263"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFA9824012.1"
                     /translation="MLQTDNLTAAQPQRIVAAQTASAQEELLERALRPKTLDDYIGQH
                     KAKEQLAIFIQAAKKRGEALDHVLLFGPPGLGKTTLAHIIAKELGVNLRQTSGPVLER
                     AGDLAALLTNLDPHDVLFIDEIHRLSPVVEEILYPALEDYRLDIMIGEGPAARSVKID
                     LPPFTLVGATTRAGMLTNPLRDRFGIVSRLEFYENRDLTTIVSRSAQLLQLDMGEEGA
                     MEVAKRSRGTPRIANRLLRRVRDFADVKNNGVIDAAVADAALSMLDVDAQGLDVMDRK
                     FLEAVLHKFGGGPVGLDNVAAAIGESTDTIEDVIEPYLIQQGFLQRTPRGRMATERAY
                     LHFGLPVEK"
     gene            complement(12863..13546)
                     /gene="rpe"
                     /locus_tag="WNA25_001264"
     CDS             complement(12863..13546)
                     /gene="rpe"
                     /locus_tag="WNA25_001264"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFA9824013.1"
                     /translation="MTAYRIAPSILSADFARLGEEVANVIAAGADLIHFDVMDNHYVP
                     NLTFGPMVCAALKPYASVPIDVHLMVEPVDDLIQSFAKAGASIITFHPEASRHIDRSL
                     SLIRDMGCQAGLVLNPATPVYVLENVLDRLDMVLLMSVNPGFGGQSFIPHTLEKIRQV
                     RAMLDRYEGKSGRRIAIEVDGGIKTDNIAAVARAGADTFVAGSAIFGKPDYKAVIAAM
                     RAELEKAAG"
     gene            13692..14126
                     /locus_tag="WNA25_001265"
     CDS             13692..14126
                     /locus_tag="WNA25_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HFA9824014.1"
                     /translation="MPLPAPCRFAKPAASFLSMALLSCQLSHAATAYIPPNDFQPNCD
                     IRRLGLTQGQHNELRKIRAAFKMAGDRARLKVMHSEHSRRRSVVEIISSDVFNRNEAR
                     DYVESRYHSSMDFAVDELEIQHRFFHILTPQQQQMWLSSCLK"
     gene            complement(14139..15002)
                     /locus_tag="WNA25_001266"
     CDS             complement(14139..15002)
                     /locus_tag="WNA25_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFA9824015.1"
                     /translation="MELNEFLDKAYAVLRRLDAVLPPEPGHTDWNALAFRWQSAGKKG
                     FLEHLPDPHVFPLSRLAGVGRQTELLVRNTEQFIAGRPANNVLMSGARGTGKSSLVKA
                     LLHEYADKGLRLIEVDKSDLIGLPYLLTLLKERPEKFIVFCDDLSFESGDETYKALKT
                     ALDGGLSQRCANVMVYATSNRRHLMPEYLDENAGTTGVRGEIHQKEAVEEKVSLSDRF
                     GLWLSFYPFDQNDYLAAVRSWLEDFDVPYDEAARTAALQWAQMRGNRSGRSAWQFACD
                     WAGRLPEQRAP"
     gene            complement(15010..15627)
                     /locus_tag="WNA25_001267"
     CDS             complement(15010..15627)
                     /locus_tag="WNA25_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFA9824016.1"
                     /translation="MFTGIVQGLGKLTAIHRPSEAFQTYVVELPQEAAENLQHGASVA
                     NNGCCLTITEIEGNRVSFDLMAETLAKTNLGLLKEGDCVNIERAARFGDEIGGHVMSG
                     HIMATVPIVEIERDGFNRTVWFALPHELKPYILTKGFVGLDGCSLTIGKVEDSRFNVH
                     LIPETLERTLFGSRKAGDRINIEIDPNTQAIVDTVERLMAQRYAK"
     gene            complement(15790..16404)
                     /locus_tag="WNA25_001268"
     CDS             complement(15790..16404)
                     /locus_tag="WNA25_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HFA9824017.1"
                     /translation="MKTSNFPKSPPALKTFALILAGGLADRMGGEDKGLALLEGRPLI
                     DRVIGKIRLQVSHIVISANRNLEEYARRSPHVFPDARQWQHFGPLSALCTAANDLQLA
                     AADWLLIVPCDMPYLPDDLVARFESVSKRTPLCNAFYVETPVTMHYNIMYIRPQILQS
                     AIPYLFSGMKTLRSWLQQQRARPVRFEFDGHFADLNTQTDLQEG"
     gene            16611..18380
                     /locus_tag="WNA25_001269"
     CDS             16611..18380
                     /locus_tag="WNA25_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HFA9824018.1"
                     /translation="MILPTRFSDGIPLSLRLKLLTGLWVGLAALSVVLTLLLSFRLEN
                     AASVIEEAGNLKMQAYRLAYMAGEGSPRAQIDNQIAEFEKSLKRISQSDAIHPLIPSD
                     NPLAYDLIQSMLIIDWQANILPPLQAYRRPTQIELYRFAGNIELFLQALENAGEKNTW
                     WLRRFQWVIMLMTLVSSVLMLFWHQIWVIRPLQALREGAERIGQRHFDIPVPEDGTPE
                     FKQVGRCFNQMALRLKTLYDDLEGQVAEQTHNLEKQNRNLTLLYRTTRDLHQSYTPRQ
                     AAEEFLNHILPAVGAQSGNICLENGSDTDISVHTAEHGKKPPLEKYHDETFPIEYQNE
                     KLGMLSLGFSDGTSLTGDDRTLLQTLIRQLGVSLAGAKQEEEKRLLAVLQERSLIAQG
                     LHDSIAQALTFLNLQVQMLETAFAENKREEAAENIGFIKTGVQECYEDVRELLLNFRT
                     KISNKEFPEAVADLFARFTQQTGITVETVWENGSFLPTQDEQLQMIFILQESLSNIRK
                     HARATHVKFTLSEYGGRFTMTIQDNGQGFDTEKIGEPTGSHVGLHIMQERAKRIRAVL
                     EIRSQAQQGTTVSLTGAPKESLP"
     gene            18377..19033
                     /locus_tag="WNA25_001270"
     CDS             18377..19033
                     /locus_tag="WNA25_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFA9824019.1"
                     /translation="MTIKIILIDDHTLFRSGIKALLSRQHGFEVIGEAADGLSGIKMI
                     SRLQPDVVLLDLDMPGMNGREALSQIISINPQQAVIMLTVSEDSDDLTECMRIGARGY
                     LLKNINADFLLESIRKAAEGDNVFSPEMTAKLVKSLISPQPAQRTQALSSLTPRELEI
                     LGYLAAGHSNKIIARHLDLAESTVKVHVQNLLRKLNLSSRVQAAVYAIRHNVPQPVPE
                     "
     gene            complement(19189..20097)
                     /locus_tag="WNA25_001271"
     CDS             complement(19189..20097)
                     /locus_tag="WNA25_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFA9824020.1"
                     /translation="MRFPLPITNAVLKITNGAMEFKSEDIGTIKLTKATFHLKKDTSL
                     TTPEGTTLLSGGTLTLSNTGISLSGTTSVFEKGTFTNGGIITLANQSYADKLTIEGNY
                     VGKDGVLKVNTEWNSPGDDQGGNSQSDLLEITGDASGKTTVISVGKDGKENIIDGSIG
                     ELSDRYKRSAAVVKVLGQDKGAETGKLNIEDAKHTYTMRDTFSGTAKTTGAGELQLVS
                     HKDEAGATEYFWTLTTPNQDKTIITPSAPAYALVPRQNLESGYAMLDTLHQRRGENQT
                     LSRDRQGNYRQDAEATDIKATKAPWV"
BASE COUNT         4671 a         5848 c         5064 g         4655 t
ORIGIN      
        1 gtgttacgca atatataagg ggttgccgtt ccgcagttgg gcggcagcat acacgttcct
       61 ttcggcgtat tctcgccgtt gcgcggcgaa tatctcgacc tgcttgccca tgcgccgtca
      121 acgggttttc agacggcatt cgatatcggg acgggctcgg gcgtacttgc cgccattttg
      181 gcgaaacagg gcattccttc cgtcatcggc acggatacca atccgagggc ggttgcctgc
      241 gcccgtgcca atattgcccg tttgggtttt gaaaaacagg ttgaaatccg cgaaaccgat
      301 ttatttcccg aagggtttgc cgacctgatt gtctgcaatc cgccctggct tcccgccaag
      361 ccgacttccg ccgtcgaatc cgcgttatac gaccccgaat ctgcgatgct ggctgcgttt
      421 ttgcgtgatg cgccaaacat ctgaatcccg acggagaaat ccgtcttatc atttccgacc
      481 tcgccgtgca tttggggctg cggtctgccg actttttgga aaaggcattt atccgggcgg
      541 gtttgcgtgt tgaggatgtt ttgaaaacca aaccggttca caaaaaagct gccgattcga
      601 acgatccgct ggcttttgcc cgaaacaggg aaactacttt cctgtgccgt ttgaaaaagg
      661 cataaggggc ggtggcgtgc ttccgacggt aggcatcgga acgcccgcgc cgccggcacg
      721 gcatcattcg ggcggattat tcttgtgaaa atacccgctc aagcatactg cccaacgccg
      781 tctgacggac tttgacggtg tttaccgcct gacaaaatgt ctttttgccg ccgaaaaata
      841 cgaatttttc cctggcgcgg gtaatggcgg tatataacag ctccttactc aatccggaca
      901 atgcatcgtc cccttcgtcc gaaggtgcgt cggaaggcgg cagcagccag acttcccgat
      961 attccgaacc ttggcttttg tggacggtca tggcgaatgc gggttcaaat tcgggcaggc
     1021 agcttaccgc taccttttta aatccgtccg catcggcaaa ataggcggca aggctgccct
     1081 gccgtccgac atcttccata atcagtccga tgtcgccgtt gaacagctca agcgcgtagt
     1141 cgttctgtct gatcataatc ggctctccgg cgaaataggc caaatgttcc ggtatgttca
     1201 ttttgcggcg tacatagctg caataggctt cgttgaagtc ttccgcatcc tgccgccaag
     1261 ctgccagaac cacgatatcc gagatgcctg cgtatgcggc ttcgatattg ccgtctttta
     1321 ccgcctgcca ataggctttg tgcgcccggt acaacctttc gacccgagca ttcggactgc
     1381 attccgaatg ttccagttcg tccggaaacc ggtcaaacaa tgcccacgcc ccttcatcgc
     1441 ccgatacggc ggcacgggca aggcagccga tgccgctgtt gtcgccgaag cggtggctga
     1501 acgacagatg ggcggtgttt tgcgccaaca cgggcggatt tgcgctgacg ctgaaaccgt
     1561 gttccggaag gaaaccggcc agcctttggt gcgtttctcc gtccaaaacg gttttttgcg
     1621 acaaaaccga cagcaccgcc ccgattccga cggacgggag ctggttttca tcgcccagca
     1681 gaatcacgcg cgcgccggtt ttgaccgctt tcaaaagttg cagcatcaat gcggtatcca
     1741 acatggaggc ttcatcgaca atcagcacgt cgaacggcag cggacggata tggtcgaacg
     1801 ccgcctgcat tttgggcggg ctcagcttaa gcagtcggtg gacggtttgc ccttccagtt
     1861 tgagcaaatg gcggcggacg gcctccggcg cgtcaaaacc gttgattgca cggtgcagcg
     1921 cgcgcgccat atgtgccgcc gctttgcccg tcggtgcggc aagcgcgata tggggaagat
     1981 tttcgttttc accgcaaatc agtgccagca gtttggcaac cgttgtcgtt ttgcccgttc
     2041 ccggccctcc ggtaatcacc ataaaaaact gcaacagtgc caaggcggcc gcatcgcgct
     2101 gtccttcgct tcccgcgcct tgaaaccatt ttgcgaggtt ttgccgcgcg cctgccgcgt
     2161 cgggggcgga cgtgccggct gccgccaagc gttttatttc ggcagccaaa tcgtattcca
     2221 actgccacat cctgcccaaa aacagccttc tgccttccaa aatcaaaggc gcggcggatg
     2281 ttccgaccac gggtgcgagt gccgacagcg cgtcagcctc gtccccgctc aaacggataa
     2341 acgaatgacc gttttgcaat gcctgaaaca ggcgttcggt acagtttgca agcacttcgt
     2401 tgcccgaacc cgcatagcgt tccaaaaaac ggattgccgc ccttgccgcc gcttgggcaa
     2461 attcatctgt ctgcagttcc atcgtattcc ttgtcgaaat gccgtctgaa ggcacggggc
     2521 ttcagacggt gcgtgttgtt tcggctgttt aggcgtttgc gccctgtctg gggtgcaggc
     2581 tgccgtcttt catgaccatc acgcgctcga agcggccggc gagttcgtcg tcgtgcgtta
     2641 cgaccaccag ccccgttccc aattccgttt tcagttccag catcatatcc aaaacattcc
     2701 ttgcgtttgc ccggtcgagg ttaccggtcg gttcgtcggc aagcaggcat ttgggttgcg
     2761 taaccaaggc gcgcgcaatg gcggcacgct ggcgttcgcc gccggacaat tcgcccgcac
     2821 ggtgcgtcga acggtgtttc agtccgacct tgtcgagcat cgccattgcc gtttctaccg
     2881 cctctttacg gctttttttg ccgatcagaa gcggcatcat cacgttttcc agtgccgaaa
     2941 attcaggcag aagatgatgg aactggtaca cgaaaccgag atggcggttg cgcaaatcgc
     3001 ccaaacgccg ctggtttaag gcacgcaaat cctcgcccat cagcagcacc ctgccttcag
     3061 acggcatatc cagcccgccc aaaatatgca gcagcgtcga tttgccgctg cccgaagagc
     3121 cgatgatgcc ggtgctttcc cccgtgcgga tttccaaatc caagccgtac agcaccggaa
     3181 cgtccaaacc gccgtcacgg tagcgtttgc ccacgccttc gcatttcaaa atcaaatcac
     3241 tcataacgca aagcctccgc cggttgggtt tttgacgcgc gccagctcgg gtacagtgtc
     3301 gccacgaaag acagccccaa ggaaatgcag gcaatcaggg caacgtcgcc catatcgaca
     3361 tcactgggca ggtagtcgat aaaataaacc tgcgaattga tgaggtggac accgagcagg
     3421 ttttcaaaaa acgccacgac cctgccgacg ttccaacccc aaagcacgcc gcagaccaca
     3481 cccgccagcg tgccgaaaaa gcctgaaaac gcgccctgca ccataaaaat cttcatcacg
     3541 ccggcagggg aaagacccaa agtccgcaaa atcgcaatgt ccgcctgctt ttccgtaacc
     3601 gccatcacca aggaagagac aaggttgaac gccgccacgg cgataatcag cgtcaggata
     3661 atgaacatca tccgtttttc cagttcgacc gcttcaaaat agctgcggtt gctgtacgtc
     3721 caatcgcgca cccaaaccgt gtccctttgc gcctccggaa tcagcgttgc cgtcaaggcg
     3781 ggggcgtttt gcggatcggc aagcttcagc cgcagccccg caacttcctt atccaagcgg
     3841 tacagtacgc gcgcgtcttg gatatgcgtc atcgccaatg agttgtccac ttcgtaaaca
     3901 cccgtcttga ccaggccgac cacggtaaac tgcttcaggc gcggcacgac tccggcaggg
     3961 gtaacgttgc cctccggcgt aattaccgta accttgccgc cgacttccgc ccccaaagcc
     4021 tccgccaaac cgacaccgag gataatgtca aactcgcccg gaatcaggtc ttcaaacttg
     4081 cctgccggca ttttgtcgcc gtattccacc actttgcgct cttcagacgg caaaatgccg
     4141 cgtatctgaa cgcccctgat ttcgcccgcg tttgccagca atgcctgatt ggaaacatag
     4201 ggtgcggcag ccaaaatgcc tttgcggttt tcggcaaacc gaagcaggtt gcgccaatcc
     4261 gtatccgtat tgtcgatata gccgatttcg gcgtgcggcg cgacattcag gagctgcccg
     4321 cgtatttctt tctgaaagcc gttcataacc gacaagacga caatcagcgc ggttacgccc
     4381 aaggcgattc cggcaatcga aaccatcgtg ataaacgaca taaagccgtt gcgctttttc
     4441 gccctgagat acctcaagcc tatccaagcc tctagagaaa acataacgct accttaaaaa
     4501 tgtctgcaaa cgtgccgccc cggacggcgg tttgggggag gcggcaaaag tttattgtac
     4561 cgtaaaaccc cggcagcgtc cgaacggcag gctgtgtaaa taaagacgcg gtatgtggca
     4621 aattcggaaa aacaggacgg aaaacttgca caacgcgccg aactacccta tccttctctt
     4681 gaaacaaaac cttttcttta aggaaaacaa tgaatatcag gaaaatctcc gctttgtgtg
     4741 ctgttgccgt ttttactgtt tcgacagcct atgccaaaga aatcaaaatc gatgccaaca
     4801 acacgcctta ttccgaagcc gacgcgcaaa agctggcggc aacggcagtc ggtatgggcg
     4861 ttaaggaacc tgtcagcctg aacggcggca gcggcagcat taccgtttcc ggcagcagcg
     4921 cggcgcagtg cgtctttacg gtcggcagcg ggggcacttt gcagattcaa gggctcaact
     4981 gcaagtaaac cgcccgaaaa aatgccgtct gaaggcttca gacggcattt tgcattggcg
     5041 gcgttacgcc ccgccttctt taatcagacg ccgttcgtac accgcctgcg ccagcgttcc
     5101 cgcatcgaca tattccaatt cgccgcccaa tgggatgccc tgcgacagcc tgctgacctt
     5161 gtaaggcagg tttttgaaaa actcggacag gacatacgct gtggcattgc cttctgcggt
     5221 aaaggcggtc gcgataatga tttcttcgat ttccccgccg tccagccgtt gcgccagcct
     5281 gtccaatgcg atggcggata cgtccattcc caatgccgta ttgatttgcc ccatcaggac
     5341 gaaatacagc ccgtcgtggc agtttgccgc ttctatattc gacacgtcgg caggcatatg
     5401 caccaccatc agccgccgcc cgtcgcgtgt ttcatcggca caaatatcgc acaatccgcc
     5461 ttcgcaaaat gtattgcacc ttgcgcaatg gcgaacctgc ctcaatgccg tctgtaaggc
     5521 atccaccagc tcttcagcct ctttgcgctt ttgctgcaac agatggtacg ccatccgctg
     5581 tgccgatttc ggcccgacgt tgggcaatac tttcagcgca ccgatcaggc gttggaatgc
     5641 atcctgtttt ttggaattca tcatactccg ccatatggga aaacggtcgg aatattccga
     5701 ccgttatgtt gtcaacaaaa gtgtcaatta ctgaccatcg ccgttgtcaa ccgattgtgc
     5761 tcctttagtc tgtttgattt ttccgttgaa atagcggatc agcaggtcga aagtattggc
     5821 agactgctgt tgcgccaaag cctgtttcgc aggaggaagc tgtgcggcaa tatcctccgg
     5881 aggcgtgact gcctgcacct cgacaatcac gggtgccggc agaccggtca gtctgacata
     5941 ggcgggtttg ccgtttgccg gttttgcttt cagcagttcc gcataagcct cgggcggcat
     6001 ggactgcctt gcctgctgcg cgcccaaaac ggacacttcc gaccatttca cgtcaactgc
     6061 cttgccgccg ttcagttggg taagcacttc ttttgccttg ttttcggcaa gtttggcggc
     6121 ttcggtacgg atataggcct gacgcaccgc atctttggct tcttcaaaca gtaggttttt
     6181 ttcttcgcgg acttctttgg cgcggacgac ccacgcggtt tcgctgttga tggtcagcac
     6241 ttcggaattg tgttttttct tcaatacgtc gtcgctgaat acggcattga ttaggttttc
     6301 gggcatgccg gacatttgtg cgtcctgcct gctcagccaa gtttcttggg tttccacttt
     6361 caaaccgctg tttttggcgg cttcggcaag cgaggaggga tgattgaacg catcgtcgcc
     6421 cagcttttct tttgccttgt tgaagtcggc aaccgccttt ttcattttca attcgttttc
     6481 gacggcggct ttttcctgct cgaaagaagg tttggcttca tgtgccggca aacgcgccac
     6541 gcgctcttca aacgcatttt tcacttccgt ttcactgacg gtctgcttgt ctgcaaaatc
     6601 cttcagattc aaggcgacat attccaattt gaccgcctgc ggcagcagat agtctttttt
     6661 gttcgcatta taaaatttct gcaaatcggc ttcagacgct ttgacttggg cgatgaactc
     6721 gtcggggttg aaagtgtgcg aacggatggt gcggttgacc tgcgtcagcc tgatcagctg
     6781 ttccgcctgc gcgtcgccga ccaatacgcc gttttggacg aggcttacca aattctgcaa
     6841 ggcaaactga tcgcggattt cttcgacaaa ctggtcttca gacatatggc gttgcgacag
     6901 gtattgactc aaaagcgcgt gactgaattt gccgtttgcg tcgtggaaat tgggatcgtc
     6961 cacaatcatc tgcttgattt gttcggaaga aaccgaaatg cccatcagct tcgcaccctg
     7021 tttcaggtag gcgcgttgca gcagggattg gaacaccgcg tcgcgccaag ggctgccgcc
     7081 gtccgcctgc tcgttctgca tggcgttgtt gattgagtgc tcgctgattt tttcgtcgcc
     7141 cacttggacg atgtagtcgg cgcccggatg ggaaaccgtg ctgacgccga agccgacaaa
     7201 agttaatgca atcaggccta ataagacttg ggcgggtgtt ctgtattttt cgatggaatg
     7261 gaacatattt tcagattggg aatgggaaat tcaagtcggg tattgtaacg gtttttatcc
     7321 ctgtctgcac ggggcttgcc ggttgaagat gccgtcgtag gtttcttcgc tgaagccgac
     7381 gtaaaccctg ctgccgcact ccaatacggg acgcttgatc aggctcggca tttcggacat
     7441 cagtttgacg gcctccgccg tcgaggacag ggctttttgc tgtgtttcgg catcgagttt
     7501 gcgccagctt gtcccgcgtt tgttgaacag ggttgccaaa ggcacttgtt ccagccacga
     7561 gcagatttcc gcttcagacg gcatctgttt tttgaaatcc cgaaacccaa actccaagcc
     7621 gtatccggca agcctgtttt tggctttttt gactgtgtcg caatttggaa tgccgtgaag
     7681 gattatcatt tggaaacctt ttgcctgaaa taataaaacc gatattttac tataagtgtc
     7741 tgaaaatttg cccgtctgtt tcagacggcg gggcggttat gttacaatcc gaaaattcga
     7801 aaaatttaat ctcttgttca ataaaggttt tacccataat gatttctacc aacggcatta
     7861 ccatgcagtt cggcgcgaag ccgctgtttg aaaacgtatc cgtaaaattc ggcgaaggca
     7921 accgctacgg cttgatcggc gcgaacggtt cgggcaaatc caccttcatg aaaatcctcg
     7981 gcggcgattt ggaacagacc gccggcgaag tggcgattga aaacggcgtg cgtttgggta
     8041 aattgcgcca agaccagttt gcctacgaag acatgcgcgt gctggacgtg gtgatgatgg
     8101 ggcataccga aatgtgggcg gcgatgaccg agcgcgatgc gatttacgcc aatcccgaag
     8161 ccaccgaaga cgactacatg aaagccgccg aactggaagc caagttcgcc gaatacgacg
     8221 gctacaccgc cgaagcgcgc gccgccgaat tattaagcgg cgtgggcatt tccgaagatt
     8281 tgcacaatgc gaaaatggcg gaagtcgccc cgggcttcaa actgcgcgta ttgctggcgc
     8341 aggcgctgtt ctccaagccg gatgtattgc tcttggacga accgaccaac aacttggaca
     8401 tcaataccat ccgctggctg gaaggcgtgt tgaaccaata cgactccacc atgatcatca
     8461 tctcgcacga ccgccacttc ttgaacgaag tttgcaccca catggcggat ttggactaca
     8521 acaccatcac tatttatccg ggcaactacg acgactatat gctcgcttcc gcccaatcgc
     8581 gtgagcgcgc cctgaaagac aatgccaaag ccaaagagaa actgcaagag ctgcaagaat
     8641 tcgttgcccg tttttccgcc aataaatcca aagcccgtca ggcaaccagc cgtctgaaac
     8701 aggccgacaa aatcaaatcg gaaatggtcg aagtcaaacc ttctacccgt caaaacccgt
     8761 atatccgttt tgaagccgat gaaaaagcca agctgcaccg tcaggctgtg gaagttgaaa
     8821 aactggccaa acgctttgaa acccagttgt ttagaaacct caacttcatc cttgaagcag
     8881 gccaacgcct cgccatcatc ggcccgaacg gcgcaggtaa atctaccttg ctgaaactct
     8941 tggccggcgc gtacaacccc gaatattcag acggcctgtt gccggacgaa ggcagcatca
     9001 aatgggcgga aaaagccagt gtcggctact atccgcaaga ccatgaaaac gactttgacg
     9061 tcgatatgga cttgagcgaa tggatgcgcc aatggggtcg ggaaggcgac gacgaacaag
     9121 tcatccgagg cactttgggg cgtttgcttt tcggcagcaa cgacgtggta aaaaaagtga
     9181 aagttctctc cggcggcgaa aaaggccgta tgctttacgg caaactgttg ctgttgaaac
     9241 ccaatgtctt ggtcatggac gaaccgacca accatatgga catggaaagc atcgaatcgc
     9301 tgaacatggc actggacaaa tacaacggca cgctgatttt cgtctcccac gaccgtcagt
     9361 tcgtttcctc cctggcaacc caaatcatcg aactggacgg caaaggcgga tatgaacact
     9421 acttgggcga ttacgaaagc tatctcgaga aaaaaggcgt agcataagcg cacccctccg
     9481 gttgaaacaa tgccgtctga agccgcttca gacggcattg ttgataactt taaaatagga
     9541 agcatatgca gacttacctc gtcggcggcg ccgtccgcga ttatcttttg ggcctgcccg
     9601 tcaaagaccg cgattgggtg gtcgtcggcg cagacgcgca aaccatgttg gcgcaaggtt
     9661 tccagccggt cggcaaagat tttcctgtat tcctccatcc caaaacgcac gaagaatacg
     9721 ccctagcccg caccgaacgc aaaaccgcca agggttacgc cggtttcagt ttccacgccg
     9781 acaaagacgt tacgctggaa caggacttga tgcgccgcga cctgaccatc aacgcgatgg
     9841 cgcaagatgc ggacggcaag attatcgacc ctttcggcgg gcaacgggat ttggcggcag
     9901 gcattttgcg ccacgtctca cccgccttcg ccgaagatcc cgtccgcatc cttcgtgccg
     9961 cccgctttgc cgcgcgttac ggctttgaaa tcgccgaaga aaccataaag ctgatgcggc
    10021 agatggtgga aaacggcgaa gcggacgcat tggttgccga acgcgtctgg caggagttgg
    10081 cgaaaggttt gatggaaaaa aatccgcgca aaatgattga aatgttgcgc gaatgcggcg
    10141 cactccaagt cttgctgccc gaagtcgacg ccctcttcgg cgtgccgcaa cgcgccgact
    10201 atcatcccga aatcgacagc ggcatccata ccctgatgac gctgcaacgc gccgccgata
    10261 tgggtttgag cctgcccgaa cgctatgccg ccctgctgca cgacttgggc aaagccaaaa
    10321 caccgcccga catcctgccg cgccaccacg gacacgacat caacggcgta gaacccgtgc
    10381 gcgaagtcaa tcaacggctg cgcgcgccga ggcagtgtgc cgagcttgcc gaattggttt
    10441 gccgttggca cattattttc caccaagtcg gacagcttaa aagccaaacc attctgaacg
    10501 tattgaaaaa aaccgatgcc ttccgccgtc ccgagcgttt tcagacggca ttgaacgtct
    10561 gcattgccga cacgcaaggc cgtctgaacc gagaacacac gccctacccc cagcgcgcgc
    10621 actggctcgc cctgctcgaa gccgccaatc aggcggattc gggcaaaatc gccgccgaat
    10681 gccgcgcgca gggaaaagcg cactttatcg ccgaacaaat cgaccgggcg cggctggcgc
    10741 aaatcgcccc gctgcaaaaa gcgtttcggg gcgcgtagga aaaagaaaac gccaaacgcg
    10801 gcatcttttg aggttcgccc gcattgccga aacagcgtcg gagaaaggga tggatgacat
    10861 cggtttattc ggctgccttt taatattccc ttgatgcagg cctgattagt cggacaaaaa
    10921 taccttttgt tcaacggaac cgatttctat ccggacggaa acagccggct ttccccttgc
    10981 aaacggatgg aatcaagcgg gtattcaaac acagccgttt atttttattc cgccgccaag
    11041 ttatgggata gggtttgcgt gtttcgggca ggcaaaccgc gcaaccttag acgcagtccg
    11101 aagttttgaa ggcggcatcc gggccgggtt ttaaaacagc cgccgccata tccgctataa
    11161 tccatccttc agccattccg cccccgacat aaaatcatga ccctgaaaac cgatttattg
    11221 cctaaaatca acaacgaaga ttatcaacgc ctcatcctca aacatagcgc ggaattcagc
    11281 ggcggagaaa cccgcctgtt gaacgaaatc ctcgaaaaat tcaatttcga cgttgttcag
    11341 gcgcaggcat tggcgcaggc ggtaatgcag caagttcgct tcgatcccaa cgcctaccac
    11401 atcgacagcg acgacgaaga caccaccggc atctgccccc actgcatcaa cccgcctatg
    11461 ccgcccctgc gcgactatct cgtttggcgc gaaacacgcg gataaaacgc ttttgaccgt
    11521 tatcttttca atgccgtctg aaacgccgcc gaccgttcgg acggcatacc cgacaaaggg
    11581 aacactatgc tgcaaaccga caacctgacc gccgcgcaac cgcaacgcat cgttgccgcc
    11641 caaaccgcct ccgcacagga agaactgctc gaacgcgccc tccgccccaa aacgctggac
    11701 gactacatcg ggcagcataa agccaaagag cagctcgcca tcttcatcca agccgccaaa
    11761 aaacgcggcg aagcactcga ccacgtcctg cttttcggcc cgcccggact gggcaaaacc
    11821 acccttgccc acatcatcgc caaagagttg ggcgtaaatt tgcgccaaac cagcggcccc
    11881 gtcctcgaac gcgccggcga cctcgccgcc cttttgacca accttgatcc gcacgacgta
    11941 ttgtttatcg acgaaatcca ccggctaagc cccgtcgtcg aggaaatcct ctatcccgcg
    12001 ctcgaagact accggctcga cattatgata ggcgaaggac ccgccgcacg ttccgtcaaa
    12061 atcgacctgc cgcccttcac cctcgtcggc gcgaccaccc gcgccggtat gctgaccaac
    12121 ccgttgcgcg accgcttcgg catcgtctcc cgcctcgagt tttacgaaaa ccgcgacctt
    12181 accaccatcg tcagccgttc ggcacaactg ttgcagctcg atatgggcga agaaggcgcg
    12241 atggaagtcg ccaaacgcag ccgcggcacg ccccgcatcg ccaaccgcct gttgcgccgc
    12301 gtgcgcgatt tcgccgacgt gaaaaacaac ggcgtaatcg atgccgccgt cgccgatgcc
    12361 gctttaagta tgctggacgt ggacgcgcag gggctggacg tgatggacag gaaattcctc
    12421 gaagccgttt tgcacaaatt cggcggcggc cccgtcggtt tggacaacgt tgccgccgcc
    12481 atcggcgaat ctacggacac catcgaagac gttatcgaac cctacctcat ccaacaaggc
    12541 tttttgcaac gcaccccgcg cggcaggatg gcgaccgaac gcgcctacct gcatttcggg
    12601 ctgcccgtcg aaaaataaac catgccgtct gaaaccgaac cgggtttcat acggcattgt
    12661 tatcagacgt ttgcaccggc accggtttca tcacttaaca gaaacctttt gtccggcagg
    12721 acagattctc cccgatgaaa ataactgttt ccctggcaaa cggttgaaac ccgtctggct
    12781 gccggttgaa aaataacgca atgctgtctg aaacagagct aattttcaga cggcatttct
    12841 atttcaatca ttggctcaag gttcagcctg ccgctttttc cagttccgcc ctcatcgcag
    12901 cgattaccgc cttgtaatcc ggtttgccga aaatcgccga accggcaaca aaggtatccg
    12961 caccggctcg ggcaacggcg gcaatattgt cagttttgat gccgccgtcc acttcaatgg
    13021 caatacgtct gccgcttttc ccttcatagc ggtcaagcat ggcgcgaacc tgtctgattt
    13081 tttccaaagt gtgcggaatg aagctctgac cgccgaaccc ggggttgacc gacatcagca
    13141 aaaccatatc cagcctgtcc aacacgtttt ccaacacata tacgggcgtt gccggattca
    13201 acaccagccc cgcctgacag cccatgtccc gaatcaggct caagctgcgg tcgatatggc
    13261 ggctcgcttc gggatggaaa gtgataatcg atgctcctgc tttggcaaac gattgaatta
    13321 aatcgtcaac gggttcgacc atcagatgca catcgatcgg cacgcttgca taaggcttca
    13381 acgccgcgca gaccataggt ccgaaggtca ggttcggcac ataatggttg tccatcacgt
    13441 caaaatggat cagatccgca cccgccgcaa tgacatttgc cacctcttct ccgaggcggg
    13501 caaagtctgc cgacaaaata ctgggtgcga tacggtaggc ggtcatattt tttccttcaa
    13561 tgcctttatg ggcggactca acgcgggaaa gcctcgaaac agcattatta acaaaagtta
    13621 actgccataa taccatcttt cacacggcaa tcaagtatat tgtccgaaat atccggatta
    13681 aaggaaaagc cgtgccgctg cctgctccct gccgttttgc caaacctgcc gcctcttttt
    13741 taagtatggc tttgctttcc tgccagcttt cccacgccgc cacggcttat atccccccga
    13801 acgattttca accgaactgc gacatacgcc ggctcgggct gacacagggt cagcacaatg
    13861 agctgcgtaa aatccgcgcc gccttcaaaa tggcgggcga cagggcgcgt ttgaaggtta
    13921 tgcattccga acacagccgc cgccgctctg tcgtcgaaat catttcttcg gatgttttta
    13981 atcggaacga ggcgcgcgat tatgtcgaaa gccgctacca ctccagcatg gattttgcgg
    14041 tggacgaatt ggaaatccaa caccgcttct tccatattct cacaccgcaa cagcagcaaa
    14101 tgtggctttc ttcctgcctc aaataatccc cgaaacactc acggcgcacg ttgctccggc
    14161 agcctgcccg cccagtcgca ggcaaactgc caagcggaac gtcccgagcg gttgccccgc
    14221 atctgcgccc actgcaacgc cgccgtccgg gcggcttcgt catatggcac atcaaaatct
    14281 tccaaccagc ttcgcactgc cgccagataa tcgttttgat cgaacggata aaaactgagc
    14341 cacaatccga atcggtcgga caaagatact ttttcttcca cggcctcttt ttgatggatt
    14401 tctccgcgca cgcctgtcgt acctgcattt tcatccaaat attcgggcat cagatgccgt
    14461 ctgttggaag tcgcgtacac catcacgttg gcgcaacgtt gggacagacc gccatccaat
    14521 gccgttttca atgccttata ggtttcgtcg ccgctttcaa atgacaaatc gtcgcaaaac
    14581 acaatgaatt tttccgggcg ttccttcaaa agtgtcaaca gatacggcag gccgatcaaa
    14641 tcgcttttat cgacttcgat caggcgcaat cccttatccg catattcgtg cagcagggct
    14701 ttgaccagcg aggatttgcc tgttccgcgc gcgccgctca tcaatacgtt attcgcaggt
    14761 ctgccggcaa taaattgttc ggtattacgc accagcaatt cggtctgcct gccgactccg
    14821 gcaagccgcg acaaagggaa aacgtgcgga tcgggcaagt gttccaaaaa accttttttg
    14881 cccgcactct gccagcggaa ggcaagcgcg ttccaatccg tatgcccggg ttcgggcgga
    14941 agcacggcat ccaaacgccg caaaacggca taggctttat cgagaaattc gttcaattcc
    15001 atctctacct cactttgcat atctttgcgc catcagccgt tcgacggtgt cgacgattgc
    15061 ctgcgtattc ggatcgattt cgatgttgat cctgtctccg gcctttctgc tgccgaacag
    15121 cgtccgttcc aaagtttcgg gaatcagatg gacattgaaa cggctgtctt cgactttgcc
    15181 tatggtcagg ctgcaaccgt ccaagccgac gaaccctttg gtcaggatat agggtttgag
    15241 ttcgtgcggg agcgcaaacc aaaccgtgcg gttgaacccg tcccgttcga tttcgacaat
    15301 gggcacggtt gccataatgt gtccgctcat gacgtgtccg ccgatttcgt cgccgaaacg
    15361 tgccgcccgt tcgatgttga cgcaatcgcc ttccttcagc agccccaaat tggtttttgc
    15421 caaagtttct gccattaaat cgaagctgac gcggtttcct tcgatttcag taatcgtcag
    15481 gcagcagccg ttattggcga ccgatgcgcc gtgttgcagg ttttctgccg cctcttgcgg
    15541 aagctcgacg acataagtct gaaacgcctc cgacgggcgg tggattgccg tcagttttcc
    15601 caatccttga acaatgcctg taaacataat cctgtttccc tgtgtcggta aaaatggtgc
    15661 aaattgtagc atttccccgc gaaaaatgcc gtctgaaact ccttcagacg gcattgtgcc
    15721 tccggttcgg gcaaaaaccg cccggtacgc cttgaccttt cctttccgcg ccggtcggcg
    15781 gtcttgcact tatccctcct gcaaatcggt ttgcgtgttc aagtcggcaa aatgcccgtc
    15841 aaactcgaat ctgaccggcc gcgccctttg ctgctgcaac cagcttctta atgttttcat
    15901 acccgaaaac agataaggaa tcgcgctttg cagaatttgc ggacggatat acataatgtt
    15961 gtagtgcatc gttaccggcg tttccacata aaacgcattg cacaacggtg tgcgtttgga
    16021 gacgctttca aagcgcgcga ccaaatcgtc cggcagatac ggcatatcgc aaggtacaat
    16081 cagcagccaa tcggccgccg ccaactgcaa atcgttggct gcggtacaca atgccgaaag
    16141 cgggccgaaa tgctgccact gccgggcatc cggaaaaaca tgcgggctgc ggcgggcgta
    16201 ttcttccaaa ttccggttgg cactgatgac gatatggctg acctgcagcc tgattttacc
    16261 gataacgcgg tcaatcagcg gtctgccttc caacagtgcc agccctttgt cttccccgcc
    16321 catacggtcg gccaggcctc ctgccaaaat caatgcgaac gtttttaatg cgggcgggct
    16381 tttgggaaag tttgaggttt tcatagtttc agtctgccgt ttccgatatt ttagaagcgc
    16441 gtttgaaaac ggcgtcgtat aaattcatgt tatttgaatt ttatcataac gtgatttaac
    16501 actgaaagca ttgaggctta agttttcctt aacctgtcct ttctgagcag ttgtttctaa
    16561 ttccaaagaa tgatattgtt tgcaccgttt aaaacgactt tcctccgaat atgatactgc
    16621 caacccgatt ttcagacggc atcccccttt ccctgcgcct gaaactcctg accggcttgt
    16681 gggtcggttt ggcggcactg tctgtcgttt taactctgct gctttctttc cgcctggaaa
    16741 atgccgcctc ggtcatcgaa gaggcgggca atctgaagat gcaggcatac cggcttgcct
    16801 atatggcggg cgaaggctcg ccccgcgcac aaatcgacaa tcagattgcc gaatttgaaa
    16861 aaagtttaaa acgcatttcc caaagcgatg ccatccatcc gctgattccc tccgacaatc
    16921 cccttgccta cgatttgata cagtccatgc tgattatcga ctggcaggcc aacatcctcc
    16981 ccccgttgca ggcataccgc cgccccacac aaatcgagct ttaccgcttt gccggcaata
    17041 tcgaactgtt tttgcaggcg ttggaaaatg ccggcgaaaa aaacacttgg tggctcaggc
    17101 ggtttcaatg ggtcattatg ctgatgacgc tggtgtcgtc cgtgctgatg cttttttggc
    17161 accagatttg ggttatccgt ccgctgcagg cgttaaggga aggtgcggaa cgcatcggtc
    17221 agcggcattt cgacatccct gttcccgaag acggtacgcc cgaattcaaa caggtcgggc
    17281 ggtgtttcaa ccaaatggcg ctcaggctga agactttata cgatgatttg gaaggacagg
    17341 ttgccgaaca aacccacaac cttgaaaagc aaaaccgcaa cctgaccctg ctgtaccgga
    17401 ccacgcgcga cctgcatcaa tcctatacgc cgcgacaggc ggcggaagaa tttttgaacc
    17461 acatcctgcc cgccgtcggc gcgcaatccg gcaacatctg cctggaaaac ggttccgaca
    17521 cagacatttc cgtccatacg gccgaacacg gcaaaaagcc ccctttggaa aaataccatg
    17581 acgaaacctt ccccatcgaa tatcaaaacg agaaactggg catgctttcg ctcggctttt
    17641 cagacggcac ttccctcacc ggagacgacc gcacgctgct tcaaaccctg attcggcaat
    17701 tgggcgtatc gctcgccggg gcgaaacagg aggaagaaaa acgtctgctt gccgtattgc
    17761 aggaacgcag cctgattgca caggggctgc acgacagcat cgcgcaggca ttaaccttcc
    17821 tgaacctgca ggtacagatg ctggaaaccg cctttgccga aaacaaacgg gaggaagccg
    17881 cagaaaacat cggctttatc aaaacaggcg tgcaggaatg ttatgaagat gtccgcgaac
    17941 tgctgctcaa tttccgtacc aaaatcagca ataaagaatt tcccgaagct gttgccgacc
    18001 tgttcgcgcg cttcacgcaa caaaccggga taacggtcga aaccgtttgg gaaaacggtt
    18061 cgttcctgcc tacacaggac gagcagctcc aaatgatttt catcctgcaa gaaagcctgt
    18121 ccaacatccg caaacacgcc cgcgccaccc atgtaaaatt caccctttcc gaatacggcg
    18181 gacgctttac catgaccatc caagacaacg gacaaggttt cgacacggag aaaataggag
    18241 aacctacggg cagccatgtc ggactgcaca tcatgcagga gcgtgccaaa cgcatccgtg
    18301 ccgttttaga aatccgttcc caagcacaac agggaaccac cgtctcattg acgggcgcac
    18361 ccaaagaaag cctaccatga ccatcaaaat catcctgata gacgaccaca ccctcttccg
    18421 cagcggcatc aaagcccttt tgtcgcgcca acacggtttt gaagtcatcg gcgaagccgc
    18481 agacggactc tcgggcatca aaatgatcag tcggctgcaa cccgatgtcg tcctgctcga
    18541 tttggatatg ccaggcatga acgggcgcga ggccttgtcc caaatcatca gcatcaatcc
    18601 gcagcaggca gtcattatgc tgaccgtttc cgaagacagc gacgacctta ccgaatgtat
    18661 gcgcatcggc gcgcgcggct acctgctgaa aaacatcaac gccgattttc ttctcgaaag
    18721 catacgcaaa gccgccgaag gcgataatgt attctcgccc gagatgaccg ccaaactcgt
    18781 caaaagcctg atttcccccc aacctgccca aaggacgcag gcactctcct cacttacccc
    18841 tcgtgaactg gaaatcttgg gctatctcgc cgcaggacac agcaacaaaa tcatcgcccg
    18901 ccacctcgat cttgccgaat ccaccgtcaa agtccacgtt caaaacctgc tccgcaaact
    18961 caacctcagc agccgggtgc aggccgccgt ttacgccatc cggcacaacg tcccccaacc
    19021 tgtgccggaa tagacgttca gacggcatcg ggtcaggaaa aaagcaatgc gcccctttga
    19081 cggggtgcaa tatataaggg acggcacacc ggatgccgtc cctttaagta cgccattacc
    19141 gccgtaaccc ggacggaatg gcttaggcag tttatttcca agaatatttc acacccacgg
    19201 tgccttggta gcctttatgt ctgttgcttc cgcatcctgc cgataattgc cttgcctgtc
    19261 ccgagaaagg gtttgatttt cgccacggcg ttggtggagc gtatccagca tcgcatagcc
    19321 tgattccaag ttttgacgtg gaacgagtgc gtaggccggt gcgcttggcg tgatgatggt
    19381 tttatcttga ttcggcgtgg ttaaagtcca gaagtattct gtcgcgcccg cctcatcttt
    19441 gtgtgaaacc aattgcaatt cccccgcccc tgtggttttg gcagttccgc taaaagtatc
    19501 gcgcattgtg taggtatgtt tagcgtcttc gatatttaat ttaccggttt ccgccccctt
    19561 gtcttgtccc aaaaccttaa caacggcggc actgcgtttg tagcggtcgg aaagttcgcc
    19621 gatcgagccg tcgataatat tttctttgcc atctttaccc actgaaatga ccgttgtttt
    19681 tcctgaagca tcgcctgtga tttccagtaa atcagattga gaatttcctc cttgatcatc
    19741 acccggcgag ttccattctg tattgacttt taacacaccg tctttaccga cataatttcc
    19801 ttcaatggtg agtttgtctg cataggattg attggcaagc gtaatgatgc cgccgttggt
    19861 gaatgttcct ttttcaaata cagaagttgt acccgataaa gaaatacctg tattagatag
    19921 ggttagcgtg ccgcctgata ataatgtagt cccttcgggg gttgttaatg atgtgtcttt
    19981 ttttaggtga aaagtagctt ttgttagttt tatggttcca atatcttctg atttgaattc
    20041 cattgcgcca tttgtaattt ttagcacagc attggtaata gggaggggga agcgcattgt
    20101 aaatgatttt tctgttccat ctggattgat taatgtacca gatgcgccaa agatctctcc
    20161 ttcagggata gaaacagttc catcagaaat tgatggttta tttgcgcttt tatcactgtg
    20221 tttatgaatt aatgtaat
//
LOCUS       DAWTQS010000036        20125 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.036, whole genome shotgun sequence.
ACCESSION   DAWTQS010000036 DAWTQS010000000
VERSION     DAWTQS010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20125
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.036"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            253..1317
                     /locus_tag="WNA25_001272"
     CDS             253..1317
                     /locus_tag="WNA25_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HFA9824021.1"
                     /translation="MKAARFYNKGDIRIEDIPEPTVAPGTVGINVAWCGICGTDLHEF
                     MEGPIFIPPCGHPHPISGESAPVTMGHEFSGVVYAVGEGVDDIKVGQHVVVEPYIIRD
                     DVPTGEGSNYHLSKDMNFIGLGGCGGGLSEKIAVKRRWVHPISDKIPLDQAALIEPLS
                     VGHHAYVRSGAKAGDVALVGGAGPIGLLLAAVLKAKGIKVIITELSKARKDKARESGV
                     ADYILDPSEVDVVEEVKKLTNGEGVDVAFECTSVNKVLDTLVEACKPGANLVIVSIWS
                     HPATVNVHSVVMKELDVRGTIAYCNDHAETIKLVEEGKINLEPFITQRIKLDKLVSEG
                     FERLIHNNESAVKIIVNPNL"
     gene            1458..1781
                     /locus_tag="WNA25_001273"
     CDS             1458..1781
                     /locus_tag="WNA25_001273"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HFA9824022.1"
                     /translation="MGDSVLSAIQQTIIQRKSADPSESYVAQLLHKGEDKILKKVIEE
                     AGEVLMASKDKDPSHLVYEVADLWFHTMILLTHHDLKAEDVLDELSRRQGLSGLAEKA
                     ARTES"
     gene            1855..2178
                     /locus_tag="WNA25_001274"
     CDS             1855..2178
                     /locus_tag="WNA25_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HFA9824023.1"
                     /translation="MDNCIFCKIAAKEIPAQTVYEDGEMVCFKDINPAAPLHLLLIPK
                     VHFDSLAHAAPEHQPLLGKMMLKVPEIAKASGLTDGFKTLINTGKGGGQEVFHLHIHI
                     MGTPV"
     gene            2225..2428
                     /gene="tatA"
                     /locus_tag="WNA25_001275"
     CDS             2225..2428
                     /gene="tatA"
                     /locus_tag="WNA25_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFA9824024.1"
                     /translation="MGSFSLTHWIIVLIIVVLIFGTKKLRNVGKDLGGAVHDFKQGLN
                     EGTDGKEAQKDDVIEHKKDEDKA"
     gene            2432..3118
                     /gene="tatB"
                     /locus_tag="WNA25_001276"
     CDS             2432..3118
                     /gene="tatB"
                     /locus_tag="WNA25_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatB"
                     /protein_id="HFA9824025.1"
                     /translation="MFDFGLGELIFVGIIALIVLGPERLPEAARTAGRLIGRLQRFVG
                     SVKQELDTQIELEELRKVKQAFEAAAAQVRDSLKETDTDMQNSLHDISDGLKPWEKLP
                     EQRTPADFGVDENGNPLPDTANTVSDGISDVMPSERSDTSAETLGDDRQTGSTAEPAE
                     TDKDRAWREYLTASAAAPVVQAVEVSYIDTAVETPVPHTTSLRKQAINRKRDFRPKHR
                     AKPKLRVRKS"
     gene            3131..3901
                     /gene="tatC"
                     /locus_tag="WNA25_001277"
     CDS             3131..3901
                     /gene="tatC"
                     /locus_tag="WNA25_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HFA9824026.1"
                     /translation="MSETQNEQTVQPLVEHLIELRRRLMWMVVGILVCFFGMMPFAQQ
                     LYTFIADPLMANLPKDTSMIATDVIAPFFVPVKVTLMAAFLVSLPHTLYQIWAFVAPA
                     LYQNEKRLITPLVLSSVSLFFIGMAFAYFLVFPVIFKFLAGVTPVGVNMATDIDKYLS
                     FILGMFVAFGTAFEVPIVVILLTKIGAVTTEQLKHARPYVIVGAFVVAAVITPPDIIS
                     QTLLAIPLILLYEAGIWFGRFFTPRSEQDGDIQPPAKT"
     gene            3945..4553
                     /gene="maf"
                     /locus_tag="WNA25_001278"
     CDS             3945..4553
                     /gene="maf"
                     /locus_tag="WNA25_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HFA9824027.1"
                     /translation="MNTLYLGSGSPRRMEILTQLGYRVVKLPAGIDETVKAGETPAPY
                     VQRMAEEKNQAALTLFCETNGAMPDFPLITADTCVFSDGIILGKPRSQAEAIEFLNRL
                     SGKQHTVLTAVCIHYRGKTSSRVQTNRVVFKPLSSEEISAYVQSGEPMEKAGAYAVQG
                     IGGIFIQSIEGSFSGIMGLPVYETVSMLQDLGYRPPLSALKP"
     gene            4562..4915
                     /locus_tag="WNA25_001279"
     CDS             4562..4915
                     /locus_tag="WNA25_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HFA9824028.1"
                     /translation="MNRQTAYFLASFSLIALIALSLSWELWIAPLRPGGSWLALKALP
                     LCLPLSGILKKKIYTYQYSSMLVLIYFAEAVMRLFDAYPAEKICAALSAVFSIIFFIS
                     CLSFVKQYKETNNVR"
     gene            4905..6719
                     /locus_tag="WNA25_001280"
     CDS             4905..6719
                     /locus_tag="WNA25_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase C-terminal domain-containing
                     protein"
                     /protein_id="HFA9824029.1"
                     /translation="MSAETTVSGARPAAKLPIYILPCFLWIGIIPFTFALRLKPSPDF
                     YHDAAAAAGLIVLLFLTAGKKLFDVKIPAISFLLFAMAAFWWLQARLMNLIYPGMNDI
                     ASWVFILLAVSAWACKSLVAHYGQERIVTLFAWSLLIGSLLQSCIVVIQFAGWENTPL
                     LQNIIVHRGQGVIGHIGQRNNLGHYLMWGILASAYLNGQRKIPAALGAICLIMQTAVL
                     GLVNSRTILTYIAAIALILPFWYFRSDKSNRRTMLGIAAAVFLTALFQFSMNAILETF
                     TGIRYETAVERVANGGFTDLPRQSEWNKALAAFQSAPIFGHGWNSFAQQTFLINAEQH
                     TIHDNFLSTLFTHSHNIILQLLAEMGISGTLLVAATLLTGIAGLLKRSLTPASLFLLC
                     ALAVSMCHSMLEYPLWYVYFLIPFGLMLFLSPAEASDGIAFKKAANLGILTASAAIFA
                     ELLHLDWTYTRLVNSFSPAADDSAKTLNRKINELRYISANSPMLSFYADFSLVNFALP
                     EYPETQTWAEEATLKALKYRPYSATYRIALYLMRQGKVAEAKQWMRATQSYYPYLMPR
                     YADEIRKLPVWAPLLPELLKDCKAFAAAPGHPETKPCK"
     gene            6909..7586
                     /locus_tag="WNA25_001281"
     CDS             6909..7586
                     /locus_tag="WNA25_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFA9824030.1"
                     /translation="MSRVLLVDDDALLTELLTEYLSAEGLNVRSVPDGEAGVQEILSG
                     QYDVVVLDSMMPKMNGLDVLKNVRARSTVPIIMLTAKGDDIDRIIGLEMGADDYVPKP
                     CTPRELLARINAILRRAQHSGEQNNAPNSISVSDVVLYPAKRQASVKDMPLELTSTEF
                     NLLEVLMRHAGQVVSKETLSVEALDRKLAKFDRSIDVHISSIRHKLGDASLIQTVRGL
                     GYLFVKN"
     gene            7599..9005
                     /locus_tag="WNA25_001282"
     CDS             7599..9005
                     /locus_tag="WNA25_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HFA9824031.1"
                     /translation="MKLFQRIFATFCAVIVCAIFVASFSFWLVQNTLAENQFNQRRTI
                     ETTLMGSIISAFKTRGDNGAREILTEWKNSPVSSAVYVIQGDEKKDILNRYIDNYTIE
                     RARLFAANNPHSNLVRIEYDRFGEEYLFFIKGWDNHQAQRLPSPLFIPGLPLAPIWHE
                     FIILSFIIIVGLLMAYILAGNIAKPIRILGNGMDRVANGELETRISQQVDDRDDELSH
                     LAIQFDKMAEKLEKLVAKERYLLHHVSHEMRSPLARMQAIVGLIQAQPQKREQYLKRL
                     EGELTRMDTLAGELLTLSRLETSNMALEKESLKLLPFLGNLVEDNQSIAQKNGQTVAL
                     SADGKIPENTTILANESYLYRAFDNVIRNAVNYSPEGSTILINIGQDHKHWIIDVTDN
                     GPGVDEMQLPHIFTAFYRADSSANKPGTGLGLALTQHIIEQHCGKIIAENIKPNGLRM
                     RFILPKKKTGFKTEKSAN"
     gene            9123..11546
                     /locus_tag="WNA25_001283"
                     /pseudo
     CDS             9123..11546
                     /locus_tag="WNA25_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
     gene            11591..11836
                     /gene="rpsP"
                     /locus_tag="WNA25_001284"
     CDS             11591..11836
                     /gene="rpsP"
                     /locus_tag="WNA25_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFA9824032.1"
                     /translation="MVVIRLARGGSKHRPFYNVIVTDSRSRRDGRFIERVGFYNPVAN
                     EKQERVRLNADRLNHWIAQGAQVSDSVAKLIKEQKAV"
     gene            11852..12361
                     /gene="rimM"
                     /locus_tag="WNA25_001285"
     CDS             11852..12361
                     /gene="rimM"
                     /locus_tag="WNA25_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFA9824033.1"
                     /translation="MTDTQNRVAMGYIKGVFGIKGWLKIAANTEYSDSLLDYPEWHLA
                     KDGKTVSVTLEAGKVVNGELQVKFEGIDDRDSAFSLRGYTIEIPREAFAPTEEDEYYW
                     ADLVGMTVVNKDDTVLGKVSNLMETGANDVLMIDGEHGQILIPFVSQYIETVDTGSKT
                     ITADWGLDY"
     gene            12361..13110
                     /gene="trmD"
                     /locus_tag="WNA25_001286"
     CDS             12361..13110
                     /gene="trmD"
                     /locus_tag="WNA25_001286"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFA9824034.1"
                     /translation="MLIQAVTIFPEMFDSITRYGVTGRANRQGIWQFEAVNPRKFADN
                     RLGYIDDRPFGGGPGMIMMAPPLHAAIEHAKAQSSQTAKVIYLSPQGKPLTHQKAAEL
                     AELTHLILLCGRYEGIDERLLQSSVDEEISIGDFVVSGGELPAMMLMDAVLRLVPGIL
                     GDIQSAEQDSFSSGILDCPHYTKPLEFQGMAVPEVLRSGNHGLIAEWRLEQSLRRTLE
                     RRPDLLEKRVLIPKESRLLNKILQEQREIQS"
     gene            13125..13490
                     /gene="rplS"
                     /locus_tag="WNA25_001287"
     CDS             13125..13490
                     /gene="rplS"
                     /locus_tag="WNA25_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFA9824035.1"
                     /translation="MNLIQQLEQEEIARLNKEIPEFAPGDTVVVSVRVVEGTRSRLQA
                     YEGVVIARRNRGLNSNFIVRKISSGEGVERTFQLYSPTVEKIEVKRRGDVRRAKLYYL
                     RGLTGKAARIKEKLPARKG"
     gene            13811..14536
                     /locus_tag="WNA25_001288"
     CDS             13811..14536
                     /locus_tag="WNA25_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFA9824036.1"
                     /translation="MSIIVENLTVSYRRRPAVHHVDITFEEHSMWAVFGPNGAGKSTF
                     LKSLMGLQPIDTGSIRLDGLTSQNIAYLPQQSDIDRSQPMTVFDLAAMGLWYEIGFFK
                     GINTAQKQRVHEALERVGMRQFARRQIAHLSNGQFQRVLFARMLVQNAKFLLLDEPFN
                     AVDARTTYELLDVLQKCHCGGHAIIAVLHDYEQVRAYFPNTLLLAREKIAAGATETIL
                     TESYLAQASAKMQQQESPDWCAS"
     gene            14571..15446
                     /locus_tag="WNA25_001289"
     CDS             14571..15446
                     /locus_tag="WNA25_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFA9824037.1"
                     /translation="MNLYDLLLAPFAEFDFMHYALASVFCLSLSAAPVGVFLVMRRMS
                     LIGDALSHAVLPGAAVGYMFAGLSLPAMGVGGFAAGMLMALLAGLVSRFTTLKEDANF
                     AAFYLSSLAIGVILISKNGSSVDLLHLLFGSVLAVDIPALQLIAAVSGLTLITLAVIY
                     RPLVLESIDPLFLKSVNGKGGLWHVVFLILVVMNLVSGFQALGTLMSVGLMMLPAITA
                     RLWARNMGTLILLSVLIALFCGLIGLLISYHIEIPSGPAIILCCSVLYLFSVILGKEG
                     GILPKWFKNHRHHTT"
     gene            15523..16437
                     /locus_tag="WNA25_001290"
     CDS             15523..16437
                     /locus_tag="WNA25_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HFA9824038.1"
                     /translation="MKHLKLTLITALLATAATAAPLPVVTSFSILGDVAKQIGGERVA
                     VQSLVGANQDTHAYHMTSGDIKKIRSAKLVLLNGLGLEAADIQRAVKQSKVSYAEATK
                     GIQPLKAEEEGGHHHDHDHDHEGHHHDHGEYDPHVWNDPVLMSDYAQNVAEALIKADP
                     EGKVYYQQRLGNYQMQLKKLHSDAQAAFNAVPAAKRKVLTGHDAFSYMGNRYNISFIA
                     PQGVSSEAEPSAKQVAAIIRQIKREGIKAVFTENIKDTRMVDRIAKETGVNVSGKLYS
                     DALGNAPADTYIGMYRHNVKALTNAMKQ"
     gene            16657..17523
                     /locus_tag="WNA25_001291"
     CDS             16657..17523
                     /locus_tag="WNA25_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="meta-pathway of phenol degradation family
                     protein"
                     /protein_id="HFA9824039.1"
                     /translation="MKNILLVFVSFVPLCVRTDLPLNIEDIMTDKGKWKLETSLTYLN
                     SENSRAALASPVYIQTGSASFIPVPTEIQENGSNTDMLAGTLGLRYGLTGNTDIYGSG
                     SYLWHEERKLDGNGKTRNKRMSDISAGISHTFLKDGKNPALIAFLESTVYEKSRNKAS
                     SGKSWLIGATTYKAIDPIVLSLTAAYRINGSKTLSDDVKYKAGNYWMLNPNISFAAND
                     RISLTGGIQWLGKQPDRIDGKKESARNTSTYAHFGAGFGFTKTAALNASARFNVSGQS
                     SSELKLGIQHTF"
     gene            17566..18105
                     /locus_tag="WNA25_001292"
     CDS             17566..18105
                     /locus_tag="WNA25_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824040.1"
                     /translation="MKKQITAAVMMLSMIAPAMANGLDNQAFEDQVFHTRADAPMQLA
                     ELSQKEMKETEGAFLPLAILGGAAIGMWTQHGFSYATTGRPASVRDVAGGLGAIPGDV
                     GAAGKVVSFAKYGREIKIGNNMRIAPFGNRTGHPIGKFPHYHRRVTDNTGKTLPGQGI
                     GRHRPWESKSTDRSWKNRF"
     gene            18087..18452
                     /locus_tag="WNA25_001293"
     CDS             18087..18452
                     /locus_tag="WNA25_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824041.1"
                     /translation="MEKPLLTPPFFLFEDVSLDIFQGLSELEKKIEPQDLMDDVYRAF
                     DSVGNILNFRIVEKEQKGFWVSTKIKTVVFDSADMPSDDLFLKCLQSSYKAYSETEPA
                     GLDKRQLMKTLIQKCGFSC"
     gene            18510..18887
                     /locus_tag="WNA25_001294"
     CDS             18510..18887
                     /locus_tag="WNA25_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824042.1"
                     /translation="MSFGYLIATSQPCELLTKSRGETFSLIMDKMDLWIYFRFCEGNI
                     YTIRKNETESCLTERGGEWLKHIYEFNRESFIFSDVLLQKGESEENFSEIVLKSIKNN
                     KILTVEVRSGLHFDLRNIYRIEM"
     gene            18890..19087
                     /locus_tag="WNA25_001295"
                     /pseudo
     CDS             18890..19087
                     /locus_tag="WNA25_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            19258..19793
                     /locus_tag="WNA25_001296"
                     /pseudo
     CDS             19258..19793
                     /locus_tag="WNA25_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359781.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         5399 a         5936 c         4439 g         4351 t
ORIGIN      
        1 tatcgggtat ccggggggga ttaaggggat attcgggtaa aattaggagg tatttggggc
       61 gaaaacagcc gaaaacctgt gtttgggttt cggatgtcgg gagggaaggg aattttgcaa
      121 aggtctcagt ttgtagtaaa atatctaaac agattcaatc ggaaacggtc aggcttcttc
      181 acgccattcc catttcaacc gccgcctgct agattcagac ggcattccta cttgaaaaca
      241 aggagcgtta caatgaaagc agcacgtttt tacaacaaag gcgacatccg catcgaagac
      301 atccccgaac cgaccgtcgc ccccggcacc gtcggcatca atgtcgcctg gtgcggcatc
      361 tgcggtaccg acctgcacga atttatggaa ggcccgattt tcatcccgcc ctgcggtcat
      421 ccgcatccga tttccggcga gtccgcgccc gtaacgatgg gacacgagtt ctccggcgtg
      481 gtttatgccg tcggcgaagg cgtggacgac attaaagtcg gccaacacgt cgtggtcgaa
      541 ccctacatca tccgcgacga cgtaccgacc ggcgaaggca gcaactacca cctctccaaa
      601 gacatgaact ttatcggctt gggcggctgc ggcggcggcc tgtccgaaaa aatcgccgtc
      661 aaacgccgtt gggtgcatcc gatttccgac aaaatcccgt tggatcaagc tgctttgatt
      721 gaacctctgt ctgtcggaca ccacgcctat gtacgcagcg gcgcgaaagc aggcgatgtc
      781 gcattggtcg gcggcgcagg cccgatcggt ttgctgttgg ccgccgtgtt gaaagccaaa
      841 ggcatcaaag tcatcattac cgagttgagt aaagcacgca aagacaaagc ccgcgaatcc
      901 ggcgttgccg actacatcct cgacccgtcc gaagtcgatg ttgttgaaga agtgaaaaaa
      961 ctgaccaacg gcgaaggcgt ggacgtggcg ttcgaatgta ccagcgtgaa caaagtgttg
     1021 gatactttgg tcgaagcctg caaacccggc gccaacttgg ttatcgtatc catctggagc
     1081 caccccgcca ccgtcaacgt ccacagcgtc gtgatgaaag aactggatgt gcgcggcacg
     1141 attgcctact gcaacgacca cgcggaaacc atcaaactgg tcgaagaagg caaaatcaac
     1201 ctcgaaccct tcatcaccca gcgcatcaag ctggacaaac tggtttccga aggcttcgag
     1261 cgtctgatcc acaacaacga atccgccgtt aaaattattg tgaatccaaa cctgtaagca
     1321 aattacattt gcaacagcac aaatgccgtc tgaaacgctc agacggcatt ttccaacgaa
     1381 acgcaacagg cgtattgccc gagagcagcc gtttagccta tcattccgaa caaacagaaa
     1441 caaacagaag gaaaatcatg ggagattccg tactatccgc catccaacaa accattatac
     1501 agcgcaaatc tgccgacccg tccgaatctt acgtcgcaca gctcctgcac aagggcgagg
     1561 acaaaatcct aaaaaaagtg attgaagaag cgggcgaagt gttgatggca tccaaagaca
     1621 aagacccgtc ccacctggtt tacgaagttg ccgacttatg gttccacacc atgattcttc
     1681 tgacacacca cgacctgaag gcggaagacg tattggacga actttcccgc cgtcaggggc
     1741 tgtcggggct ggccgaaaaa gccgcccgta cagaatcctg aatttatatt acaatctcat
     1801 cccttccacg tttaatccgt ctgcgtgctt ttttcagacg gcaacggagc cgttatggac
     1861 aactgtattt tctgcaaaat cgccgccaaa gagattccgg cgcaaaccgt ctatgaagac
     1921 ggcgaaatgg tttgtttcaa agacatcaac cccgccgctc cgcttcatct gctgctgatt
     1981 cccaaagtcc attttgattc gttggcacac gccgcgcccg aacatcagcc ccttttggga
     2041 aaaatgatgc tgaaagttcc cgaaatcgcc aaagcttcgg gactgaccga cggtttcaaa
     2101 acccttatca ataccggcaa aggcggcgga caagaggtct tccacctgca tatacacatc
     2161 atgggcacac ccgtataaac cgttatttca caatcaaccc ctaatactta cttaaggata
     2221 catcatgggc agtttttctc tgacgcactg gattatcgta ctgattatcg tcgttttgat
     2281 attcggcacc aaaaaattgc gcaacgtcgg caaagacctc ggcggtgcgg ttcatgactt
     2341 caaacagggg ctgaacgaag gcacagacgg caaagaagcc caaaaagacg atgtaatcga
     2401 acacaaaaaa gacgaagaca aagcataact tatgtttgat ttcggtttgg gcgagctgat
     2461 ttttgtcggc attatcgccc tgattgtcct tggtccagaa cgcctgcccg aagccgcccg
     2521 cactgccgga cggcttatcg gcaggctgca acgctttgta ggaagcgtca aacaagaact
     2581 tgacactcaa atcgaactgg aagagctgag gaaggtcaag caggcattcg aagctgccgc
     2641 cgctcaggtt cgagacagcc tcaaagaaac cgatacggat atgcagaaca gtctgcacga
     2701 catttccgac ggtctgaagc cttgggaaaa actgcccgaa cagcgcacgc ctgccgattt
     2761 cggtgtcgat gaaaacggca atccccttcc cgatacggca aacaccgtat cagacggcat
     2821 ttccgacgtt atgccgtctg aacgttccga tacttccgcc gaaacccttg gggacgacag
     2881 gcaaaccggc agtacagccg aacctgcgga aaccgacaaa gaccgcgcat ggcgggaata
     2941 cctgactgct tctgccgccg cacctgtcgt acaggccgtc gaagtcagct atatcgatac
     3001 tgctgttgaa acgcctgttc cgcacaccac ttccctgcgc aaacaggcaa taaaccgcaa
     3061 acgcgatttt cgtccgaaac accgcgccaa accgaaattg cgcgtccgta aatcataaag
     3121 ggggcatccg gtgtccgaaa cacaaaacga acaaaccgtc caaccgcttg tcgaacacct
     3181 catcgagcta cgccgccgcc tgatgtggat ggttgtcggc attctcgtct gctttttcgg
     3241 catgatgccg tttgcccagc agctctatac ttttatcgcc gacccgctga tggcaaacct
     3301 gcccaaagac accagtatga ttgcgaccga tgtcatcgca ccgtttttcg ttccggtcaa
     3361 agttaccctg atggcggcgt ttttggtttc actgccgcat acgctctacc aaatctgggc
     3421 attcgtcgcc cccgcactct accaaaacga aaaacgcctg attacgccgc tcgtcctctc
     3481 cagcgtcagc ctgtttttca tcggcatggc atttgcctat ttcctggttt tccccgtcat
     3541 tttcaaattc cttgccggcg taacccccgt cggggtcaat atggcgaccg acatagacaa
     3601 atacctgtcc tttatcttag gtatgttcgt tgcgttcggc acggcatttg aagtgcccat
     3661 cgtcgtcatc ctgttaacca aaatcggcgc agtcacgacc gagcagctca aacacgcccg
     3721 gccttatgtc attgtcggcg cgtttgtcgt tgccgcagtc atcacgccgc ccgacattat
     3781 ttctcagacc ctgcttgcca ttccgctgat cctcttatac gaggcgggca tttggttcgg
     3841 acgcttcttc actccacgtt cagaacagga tggcgacata cagccgcctg caaaaacctg
     3901 acactatgcc gtctgaagcc ccgccgcaca ttaaggagca caccgtgaac accctttact
     3961 taggttcggg cagcccgcgc cggatggaaa tcctgacaca gttgggctat cgggtcgtca
     4021 agctgcccgc cggcatcgac gaaaccgtta aagccggcga aacgcccgcc ccttacgtcc
     4081 aaaggatggc ggaagaaaaa aatcaggcgg ctttgaccct cttttgcgaa accaacggcg
     4141 caatgcccga tttccccctg attaccgccg acacctgcgt cttttcagac ggcatcatat
     4201 tgggcaaacc tcgctcccaa gccgaagcaa tcgaattttt aaaccgattg tccggcaaac
     4261 aacataccgt cctgactgct gtctgcattc attatcgcgg caaaacgtca agccgcgtcc
     4321 aaaccaaccg cgtcgttttc aagcccctga gttcggaaga aatttccgcc tatgtgcaaa
     4381 gcggcgaacc gatggagaaa gccggtgcct acgccgtgca aggcataggc ggtatcttta
     4441 tccaatctat cgaaggcagc ttcagcggca ttatggggct gcccgtttat gaaaccgttt
     4501 ccatgttgca ggatttggga taccgccccc ccttatccgc ccttaaaccg taaagtccgc
     4561 cgtgaacaga caaaccgctt acttccttgc ctccttcagc ctgattgcac tgattgccct
     4621 atccctttct tgggaattat ggattgcacc gttacgcccg ggcggttcgt ggctcgcgct
     4681 caaagccctg cccctctgcc tgccgctttc aggtattcta aagaaaaaaa tctataccta
     4741 ccaatacagc tccatgctgg ttctgattta ctttgccgaa gccgtcatgc gcctgttcga
     4801 cgcctatccc gcagaaaaaa tttgcgccgc gctttccgca gtattcagca tcatcttctt
     4861 catatcctgc ctgtcgttcg tcaaacaata caaggaaaca aacaatgtcc gctgaaacga
     4921 ccgtatccgg cgcgcgcccc gccgccaaac tgccgattta catcctgccc tgcttccttt
     4981 ggataggcat catccccttt accttcgcac tcaggctgaa accgtcgccc gacttttacc
     5041 acgatgccgc cgccgcggcc ggcctgattg tcctgttgtt cctcacggca ggaaaaaagc
     5101 tgtttgatgt caaaatcccc gccatcagct tcctcctgtt tgcaatggcg gcattttggt
     5161 ggcttcaggc acgcctgatg aacctgattt atcccggaat gaacgacatc gcctcttggg
     5221 ttttcatctt gctcgccgtc agcgcgtggg cctgcaagag tttggtcgca cactacggac
     5281 aagaacgcat cgttaccctg tttgcctggt cgctgcttat cggctccctg cttcaatcct
     5341 gcatcgtcgt catccagttt gccggctggg aaaacacccc cctgcttcaa aacatcatcg
     5401 ttcacagagg gcaaggcgta atcggacaca tcgggcagcg caacaacctc ggacactacc
     5461 tcatgtgggg catactcgcc tccgcctacc tcaacggaca acgaaaaatc cccgcagccc
     5521 tcggcgcaat ctgcctgatt atgcagaccg ccgttttagg tttggtcaat tcgcgcacca
     5581 tcttgaccta catagccgcc atcgccctca tccttccctt ctggtatttc cgttcggaca
     5641 aatccaacag acggacgatg ctcggcatag ccgcagccgt attccttacc gcgctgttcc
     5701 aattttccat gaacgccatt ctggaaacct ttacaggcat ccgctacgaa actgccgtcg
     5761 aacgcgtcgc caacggcggt ttcacagact tgccgcgcca aagcgaatgg aataaagccc
     5821 ttgccgcctt ccagtccgcc ccgatattcg ggcacggctg gaacagtttt gcccaacaaa
     5881 ccttcctgat caatgccgaa cagcacacca tacacgacaa cttcctcagc accttgttca
     5941 cccattccca caacatcatc ctccaactcc ttgcagaaat ggggatcagc ggcacgcttc
     6001 tggttgccgc aaccctgctg acgggcattg ccgggctgct gaaacgctcc ctgacccccg
     6061 catcactttt cctgctgtgc gcgcttgccg tcagtatgtg ccacagtatg ctcgaatatc
     6121 ctttgtggta tgtctatttc ctcatcccct tcggactgat gctctttctg tcccccgcag
     6181 aggcttcaga cggcatcgcc ttcaaaaaag ccgccaatct cggcatactg accgcctccg
     6241 ccgccatatt cgcagaattg ctgcacttgg actggacata cacccggctg gttaactcct
     6301 tttcccccgc cgctgacgac agtgccaaaa ccctcaaccg gaaaatcaac gaactgcgct
     6361 atatttccgc aaacagcccg atgctgtcct tttatgccga cttctccctc gtaaacttcg
     6421 ccctgccgga ataccccgaa acccagactt gggcggaaga agcaaccctc aaagcactaa
     6481 aataccgccc ctactccgcc acctaccgca tcgccctcta cttgatgcgg caaggcaaag
     6541 ttgcagaagc aaaacaatgg atgcgggcaa cacagtccta ttacccctac ctgatgcccc
     6601 gatacgccga cgaaatccgc aaactgcccg tatgggcacc gctgctgccc gaactgctca
     6661 aagactgcaa agccttcgcc gccgctcccg gccatccgga aacaaaaccc tgcaaatgac
     6721 ccgcgtcggc ggatgcggat accgcccgaa atgtaaagct ccatgcaaga cattgcaaaa
     6781 aacagcaaac cggtagggaa atacgctatc aaaaaacatt gcagccgtgt taagataaac
     6841 tgtcaaacaa tcttttcacg ccacgcccga aacagggtcg gggcataccc ttacgaaaag
     6901 gaaacaccat gagccgcgta ttactcgtag atgacgatgc cctgctgacc gaactgctga
     6961 ccgaatacct gagcgccgaa ggtctgaacg tccgcagcgt tcccgacggg gaagcaggcg
     7021 tacaggaaat cctgagcggg caatacgatg tagtcgtatt ggattccatg atgcccaaaa
     7081 tgaacggctt ggatgtcttg aaaaacgtac gcgcccgaag caccgtcccc atcatcatgc
     7141 tgaccgccaa aggcgacgac atcgaccgaa tcatcggctt ggaaatgggt gcggacgact
     7201 atgtccccaa accctgcaca ccacgcgaac tcttggcacg catcaatgcc atcctccgcc
     7261 gagcacaaca cagcggcgaa cagaacaacg cacccaacag catctccgtc agcgatgtcg
     7321 tcctataccc cgccaaacgc caggcatccg tcaaagacat gccgctcgaa ctgaccagca
     7381 ccgaattcaa cctgctcgaa gtcctgatgc gccatgccgg acaggtagtc agcaaagaaa
     7441 ccctgtccgt cgaagcactc gaccgcaaac tggcaaaatt cgaccgcagt atcgacgtac
     7501 acatctccag catccgccac aagctgggcg atgcctccct gattcaaacc gtacgcggct
     7561 tgggctacct gtttgtcaaa aactgaagca gcgggcaaat gaaactgttc caacgcattt
     7621 tcgccacatt ttgcgcggtt atcgtctgcg caatctttgt ggcgagtttt tctttttggc
     7681 tggtgcagaa cacccttgcc gaaaaccaat tcaaccaacg ccgcaccatc gaaaccacat
     7741 tgatgggcag cattatttcc gcattcaaga cacggggcga caacggcgcg cgcgaaatcc
     7801 tgaccgaatg gaaaaacagc cccgtctcat ccgccgttta cgtcatacag ggcgacgaga
     7861 aaaaagacat cttaaaccgc tatatcgaca attacaccat agaacgcgcc cggttgtttg
     7921 ccgccaacaa cccccattcc aaccttgtcc gcatcgaata cgaccgtttc ggcgaagaat
     7981 acctgttctt cattaaaggc tgggacaacc accaggcaca acgcctgccc agcccgctgt
     8041 ttatcccggg cctgccgctt gccccgattt ggcacgaatt catcatcctc tccttcatca
     8101 tcattgtcgg actgctgatg gcatatatcc ttgccggcaa cattgccaaa cccatcagaa
     8161 tcttaggcaa cggcatggac agggtggcaa acggagaact tgaaacccgc atctcccaac
     8221 aggtcgacga ccgcgacgac gaattgtccc atcttgccat ccaattcgac aaaatggcag
     8281 aaaaactcga aaaacttgtc gccaaagaac gttacctact ccaccacgtt tcccacgaaa
     8341 tgcgctcccc gcttgcccgt atgcaggcaa ttgtcggact gattcaggca cagccccaaa
     8401 aacgggagca atatctcaaa cggctggaag gcgaactgac ccgcatggat acgctggccg
     8461 gggaactgtt aaccctgtcc cgcctcgaaa cctccaatat ggctttggaa aaagaaagcc
     8521 tgaaactcct gcccttcctg ggcaacctgg tagaagacaa ccaaagtatc gcccaaaaga
     8581 acggacagac ggtcgccctg tctgccgacg gaaaaatccc cgaaaacaca accatccttg
     8641 ccaacgaaag ctacctgtac cgcgccttcg acaacgtcat ccgcaacgcc gtcaactaca
     8701 gtcccgaagg cagcaccatc ctgatcaaca tcggacaaga ccacaaacac tggataatcg
     8761 acgttaccga caacggcccc ggcgtggacg aaatgcagct cccgcacatc ttcaccgctt
     8821 tctaccgtgc agactccagc gccaacaaac ccggaacagg actggggctt gcattgaccc
     8881 aacatattat tgaacagcac tgcggcaaaa tcatcgccga aaacatcaaa ccgaacggtc
     8941 tgcggatgcg ctttatcctg cccaagaaaa aaaccggttt caaaacagaa aaaagtgcga
     9001 actgaccata atgccgtctg aacccgcttc agacggcatt gcacaaacag ttaccccaaa
     9061 acaaacacac agcgggtgca tctccaaaaa tcacgcacat accgtccgca aaggaatata
     9121 tcatgccggc acaaaccgat ccgggctact tcttcatgcc caaccacatt atcctgatag
     9181 gcgcgagcga acaaccgtac agcctgggtg aacgcgtact cagcaacctg ctgagtacgc
     9241 cctttcaagg aaaaatcacc cccgtaaacc cgcgccacca caccatagcc gggcttcccg
     9301 cctacaccag cctcaacaaa atccccggca gtgcagacct gattattacc gttaccccgc
     9361 ccgacagtta cgacaccctc ctcaaaacct gccgtaaaaa gcagctccga cacatcatcc
     9421 tcatacagga ctgggacagc ctgtctgccg cagaactgca caccgccgaa actgccatcc
     9481 gcaaacacca cggcaacgga ctcaacatca ccgcctacac caccgcaggc atccaactgc
     9541 cctcactcgg actcaacatc agtacccaag acggatatgc cgcaggccat accgccatac
     9601 tgaccggcaa tgccgccgtc agccgccaaa tcgacaacat cctgaacaaa ctccgtcaag
     9661 gcacatcccg ccacatcaac ctgcatcccg gcatcagccc catcacatcc gccgattggc
     9721 tcaaccgctt cggacacagc ctgcacacca aaaccgccgt cctacaccac aaccctgaag
     9781 aggatcagcg caggctgttc agcgcaatcc gccaatttac ccgccatacg ccgctgattc
     9841 tccacatcac ctgcctcacg acagaaaccg accgtgccgt actgcactgc ctcgcccgac
     9901 actgcaactt cctcatcagt ttcaacgccg acgacctcga agccgcactg agcgcccaac
     9961 tgtccgacct tccacccctg tcccgactcg acatcctgtc cgacacgcct gccgaatggc
    10021 tgcacgcgca cgcaccaaaa aacctcaccc tccactttcc caaccttccc caccacatcc
    10081 gcaacggaca cctgaccggc acacccacac cctcaatctg ccacgacatc gcctcacgtc
    10141 agcttgcccg ccccgacacc caagccgtcc tgaccatcct cggtccctcc ggacacgagg
    10201 attacaaaaa aacagcacgc gcccttatcc gcctgtccga acagaccgcc aaacccctgc
    10261 tcgtcaacag ccccttttca gacggcataa cacatttcga cacccccgca caggcaatcc
    10321 gcaccctttc ctaccgtaac accgccgccg ccctgaaaca ggcacagctc gacattgcac
    10381 cgccgcagcc atgccgtctg aaaacaccgc aaccccaaaa catcaaaaaa gcccttgcag
    10441 cggcaaatcc cgccctgctc gccgaagccc tgcacctccc cctaccggca caccacccat
    10501 aacgccgtcc aattccaatt cggcagccac cccctctatg gcgacatcct gaccgcacgc
    10561 tgcaacggac aaaccactgc cgtactcccg ccgtttacca cgctcgacag ccgccacctt
    10621 gcccgctttg ccgaactcga cggcacacaa accctcgatc agttcctgca cacactgacc
    10681 gtcatccccg aataccgcca acacattttc ggcatcaccc tcaacctcaa cggcggacaa
    10741 tacagcagcg atttcatctt aaagacaccc ggaacacacg acacgcccaa acgcaagaac
    10801 acaggcaaag ccgcccaaac cctcgaacat gccgccgcaa aaatgcagag tgccgccgca
    10861 tacctggaac acaaaaaccc gacagccgcc gaatttctcc gccacacaag cgaagccgcc
    10921 gcagaactgc tcggcagcaa aaccgaaacc ggagcagccg tacccaacgt acttgccccc
    10981 tatcccgcag cacaccccaa aacactgtcc ctaaaaaaca acacgaccgt taccattatc
    11041 ccccttttac ccgaagacgc agaagccaaa cagcagttcg tccgcagcct cggccccgaa
    11101 gcacggtaca cacgcttcat gacccacacc aacgaactgc ccgcagccac gttggcacgc
    11161 ctgtgcaacc ccgattacca ctgtgaaccg catggacggc aagggatgcc gacagcaaca
    11221 tcgtcgccgt cgtccgccac agccgcctga accgcaacga atgcgaattt ggcatcacac
    11281 tggcggaaca tatgcgcggc agcgggctgg cacagaaaat gatggaactc atcatccaaa
    11341 ccgccgcaca gcaaggctac ctgagcatga gtgccgacat tctcaaaacc aataccccca
    11401 tgatcaaact tgctgaaaaa tcaggattta ccctcaagga atcggacacc gaaaaaaacc
    11461 tgtaccgcgc atatctgaac cttgcggcag acaaaacaac aggaaaaaca aataaaaact
    11521 tgcgcaccgg ccacaaaata acctaaaatc ggcagtttcc atatatccga ttttctcaaa
    11581 aggactcaaa atggtagtta tccgtttggc acgcggcggc tcgaaacacc gccccttcta
    11641 caacgtcatc gttactgact cacgcagccg ccgcgacggc cgcttcatcg aacgcgtagg
    11701 cttctacaac cccgtagcca acgaaaaaca agagcgcgtc cgcctcaatg cagaccgcct
    11761 gaaccactgg attgcacaag gcgcgcaagt cagcgactcc gttgcaaaac tgattaaaga
    11821 acaaaaagcc gtctaatccg cgtttgccgc catgacagac actcaaaacc gggtagccat
    11881 gggctacatc aaaggcgtat tcggcataaa aggctggctg aaaattgccg ccaacaccga
    11941 atattccgac agccttttgg actaccccga gtggcatttg gccaaggacg gcaaaaccgt
    12001 cagcgttacc cttgaagccg gaaaagtcgt caacggcgaa ctccaagtca aattcgaagg
    12061 catagacgac cgcgattcag cattctcatt gcgcggttac accatcgaaa taccccgtga
    12121 agcattcgcc ccgacagaag aagacgaata ctactgggca gacttggtcg gcatgaccgt
    12181 cgtcaacaaa gacgataccg ttttaggcaa ggtaagcaac ctgatggaaa ccggcgcaaa
    12241 cgacgtattg atgattgacg gagaacacgg gcagattctg attccgttcg tttcccaata
    12301 tatcgaaacc gtcgataccg gcagcaagac cattactgcc gactggggtt tggactactg
    12361 atgcttatcc aggcagttac cattttcccc gaaatgttcg acagcattac ccgctacggc
    12421 gtaacgggac gcgcgaacag acagggaatc tggcagtttg aagcagtcaa tccccgaaag
    12481 tttgccgaca acagattggg ctatatcgac gaccgcccgt tcggcggcgg tccgggaatg
    12541 attatgatgg ctccgccgct tcatgcggcg atagaacacg ccaaagcaca atcttcccaa
    12601 accgcaaaag tcatctacct cagcccccaa ggaaaaccgc tgacacacca aaaagcggca
    12661 gaactggcag aacttacgca tctgattctg ctgtgcggac gctatgaggg aatagacgaa
    12721 agactgctgc aaagcagcgt cgatgaagaa atcagcatcg gagacttcgt cgtttccggc
    12781 ggagagcttc ccgccatgat gctgatggat gcggtattga ggctcgtacc cggcatattg
    12841 ggcgacattc agtctgccga acaggattcg ttctcaagcg gtattttgga ctgcccccac
    12901 tacaccaaac ccttagaatt tcaaggcatg gctgttccgg aagtattgcg ctccggaaat
    12961 catggcttga tagcggaatg gcggttggaa caatcgctgc gccgcacctt ggagcgcaga
    13021 cccgatcttt tggaaaagcg cgttttaatc ccaaaggaat cccgcctctt gaataaaatc
    13081 ctacaagagc aacgggaaat ccaatcataa tttaggaaaa aacaatgaac ctgattcaac
    13141 agctcgagca agaagaaatt gcccgcctga acaaagaaat ccccgaattc gcaccgggcg
    13201 acaccgtagt cgtatccgta cgcgtcgtgg aaggtacccg cagccgtctg caagcctacg
    13261 aaggcgtggt tatcgcccgt cgcaaccgtg gtttgaacag caacttcatc gtccgcaaaa
    13321 tctccagcgg cgaaggtgtt gaacgtactt tccaactata ttcccctact gttgagaaaa
    13381 tcgaagtcaa acgccgtggc gacgtacgcc gtgccaaact gtactacctg cgcggtctga
    13441 ccggcaaagc tgcacgcatc aaagaaaaac tgcctgcacg caaaggttga ttcaaaccgt
    13501 tttcccccaa tgccgtctga acgttcagac ggcatttttt attgccgcga tgccggcaag
    13561 gcaacgatat gcctgaccct catccacctt aattaacaat cactcccgcc tattcaagca
    13621 gaatttacag actaaacgtt atacagtatc atatccgcca tataacgata accgctttgc
    13681 caacttccct ttcaaaactg caaatcatta tgacaccttc cctcttactg tcaggattga
    13741 ccttccgact cttcctcgcc ctgattgccg tattcctttt atggggtgtt tacctttggg
    13801 cggtatcggt atgagcatca tcgttgaaaa cctgacggtc agctaccgcc gccgccccgc
    13861 cgtacaccac gtcgacatta cttttgaaga acatagtatg tgggcggttt tcggtcccaa
    13921 cggcgcagga aaatctacct ttctcaaatc cttaatggga ttgcagccta tcgatacggg
    13981 cagcatccgg ctggacggat tgaccagtca aaacattgcc tatctccccc aacaatccga
    14041 catcgaccgt tcccagccta tgaccgtttt cgacttggcg gcaatggggc tgtggtatga
    14101 aatcggtttt ttcaaaggga taaataccgc tcaaaaacaa cgcgttcacg aagccttgga
    14161 gcgtgtcggt atgcggcagt ttgcccgccg ccaaatcgcc cacctctcaa acggacaatt
    14221 ccaacgcgtc cttttcgccc ggatgctggt tcaaaatgca aaattcctgc ttttggacga
    14281 acctttcaat gctgttgatg cacggacaac ctacgagctt ctcgacgtgt tgcaaaaatg
    14341 ccattgcggc ggacacgcta tcattgccgt tttgcacgat tacgaacaag tccgtgccta
    14401 ctttcccaat accctgctgc tcgcccgcga aaaaattgcg gcaggcgcaa ccgagaccat
    14461 tctgacagaa tcctacctcg cccaagccag cgccaaaatg cagcaacagg aaagccccga
    14521 ctggtgcgcc tcataaatac tgcccgaaac cacgccccac accagacacc atgaacctct
    14581 acgacctgct ccttgccccc tttgcagaat tcgacttcat gcactacgcc ctcgcatccg
    14641 tcttctgcct gtccctcagc gccgcacccg tcggcgtatt cctcgtcatg cgccgtatga
    14701 gcctgatagg cgacgcattg agccacgccg tcctgcccgg tgccgccgtc ggctacatgt
    14761 ttgccggctt gagcctgccc gctatgggtg tgggcgggtt tgccgccggt atgctgatgg
    14821 cgctgcttgc cggactcgtc agccgcttta ccaccctgaa agaagatgcc aactttgccg
    14881 ccttttacct gagcagcctc gccatcggcg taatcctcat cagcaaaaac ggcagcagcg
    14941 tcgatttact ccacctcctt ttcggatctg tgcttgccgt cgatattccc gcactgcaac
    15001 tcatcgccgc cgtctccggc ctcacgctca ttacccttgc cgtcatctac cgccccctgg
    15061 tgctagaaag catagacccc ctgttcctca agtccgtcaa cggcaaaggc gggctttggc
    15121 acgtcgtttt cctcatcctc gtcgttatga acctcgtatc cggcttccaa gctctcggca
    15181 ccctgatgtc ggtcggactt atgatgctgc ccgccattac cgcccgctta tgggcaagaa
    15241 atatggggac gctcattctg ttgtccgtcc tcatcgccct tttttgcggt ttgatcgggc
    15301 tgctcatttc ctaccacatc gaaatccctt ccggccccgc catcatcctc tgttgcagcg
    15361 tcctttatct cttttccgtc atactcggca aagaaggcgg catcttgccc aaatggttca
    15421 aaaaccaccg ccaccacacc acctgacccc gcaagccgct tgaacaaaca ggcggcttgt
    15481 tttaccataa tgaatgttat gttatatcac agaagaacag ttatgaaaca cctcaaactc
    15541 acccttatta ccgcattgct ggccaccgcc gcaactgccg caccccttcc ggttgtaacc
    15601 agtttcagca ttttaggcga cgtagccaaa caaatcggcg gtgagcgcgt agccgtacaa
    15661 agcctcgtcg gagccaacca agatactcat gcctatcaca tgaccagtgg cgacattaaa
    15721 aaaatccgca gtgcaaaact cgtcctgctc aacggcttgg gacttgaagc cgccgacatc
    15781 caacgcgccg tcaaacagag caaagtatcc tatgccgaag cgaccaaagg catccaaccc
    15841 ctcaaagccg aagaagaagg cggacaccat cacgaccacg atcatgacca cgaaggacac
    15901 caccacgacc acggcgaata tgacccccac gtctggaacg accctgttct tatgtccgac
    15961 tatgcccaaa acgtcgctga agccctgata aaggccgatc ccgaaggcaa agtttattat
    16021 caacaacgct tgggcaacta ccaaatgcag cttaaaaaac tgcacagcga cgcacaagcc
    16081 gcatttaatg ccgtccctgc cgccaaacgc aaagtcctga ccgggcacga cgcattttcc
    16141 tacatgggca accgctacaa catcagcttc atcgccccgc aaggcgtgag cagcgaagcc
    16201 gagccgtccg ccaaacaagt cgccgccatc atccggcaaa tcaaacgcga aggcatcaaa
    16261 gccgtattta ccgaaaatat caaagacacc cgcatggttg accgcatcgc caaagaaacc
    16321 ggcgtcaacg tcagcggcaa actgtattcc gacgcactcg gcaacgcgcc cgcagacacc
    16381 tacatcggca tgtaccgcca caacgtcaaa gccttgacca acgcgatgaa gcaataaaac
    16441 cgtttgattg gtttgaattt gaaaaggcaa aaggccgtct gaaaattaaa tacgaggttt
    16501 tcagacggcc ttctcttgtg caataaagca gtaaacaaat attgataaga aaaaaacaat
    16561 cagtattgaa aatatcgtta aaaatattat ttagacagat tacatggaat ttcattaaca
    16621 tcagtagact ttgatttcaa tcaagaaaac aatgtcatga aaaatattct cttagtattt
    16681 gttagctttg tgccattatg tgtccgcact gatctgccgc tgaatatcga agacataatg
    16741 accgacaagg gaaaatggaa actggaaact tcccttacct acctgaacag cgaaaacagc
    16801 cgcgccgcac ttgcctcacc ggtttacatt cagaccggct ccgcttcctt tatccccgtc
    16861 ccgaccgaaa ttcaggaaaa cggcagcaat accgatatgc tcgccggcac gctcggtttg
    16921 cgctacggac tgaccggcaa taccgacatt tacggcagcg gcagctatct gtggcacgaa
    16981 gaacgcaaac tcgacggcaa cggcaaaacc cgcaacaaac ggatgtccga catatccgcc
    17041 ggcatcagcc acaccttcct taaagacggc aaaaatcccg cactcatcgc tttcctcgaa
    17101 agcacggttt acgaaaaatc gcgcaacaaa gcctcgtcgg gaaaatcgtg gctcatcggc
    17161 gccaccacct acaaagccat agatccgatt gtcctttccc tcaccgccgc ctaccgcatc
    17221 aacggcagca aaaccctttc agacgacgtc aaatacaaag caggcaatta ctggatgctg
    17281 aatcccaaca tctcatttgc cgccaacgac agaatcagcc tgaccggagg catccaatgg
    17341 ctgggcaaac agcccgaccg catagacggc aaaaaagaat ccgcaagaaa cacatccacc
    17401 tacgcccatt tcggcgcagg tttcggtttc accaaaaccg cggctttaaa cgcatccgca
    17461 cgtttcaacg tttcagggca aagcagttcc gaactgaaat tgggcataca gcatacattt
    17521 taagcagatt ttgattttcc aaccttgtaa tccaaaggag ttattatgaa aaaacaaatc
    17581 accgcagccg taatgatgct gtctatgatc gcccccgcaa tggcaaacgg attggacaat
    17641 caggcatttg aagaccaagt gttccacacg cgggcagatg cgccgatgca gttggcggag
    17701 ctttctcaga aggagatgaa ggagactgaa ggggcttttc ttccattggc tatcttgggt
    17761 ggtgctgcca ttggtatgtg gacacagcat ggttttagtt atgcaacgac aggcagacca
    17821 gcttctgtta gagatgttgc tggcggatta ggcgcaattc ctggtgatgt aggtgctgca
    17881 ggaaaggttg tttcctttgc taaatatgga cgtgagatta aaatcggcaa taatatgcgg
    17941 atagcccctt tcggtaatag aacaggtcat cctattggaa aatttcccca ttatcatcgt
    18001 cgagttacgg ataatacggg caagactttg cctggacagg gaattggtcg tcatcgccct
    18061 tgggaatcaa aatctacgga cagatcatgg aaaaaccgct tctaactcct cctttttttc
    18121 tatttgaaga tgtgtctttg gatatatttc aaggattgtc ggaattggaa aagaaaatag
    18181 agccgcagga tttgatggat gatgtttacc gtgcttttga ttcggttgga aatattctga
    18241 atttccgtat agtagaaaaa gagcaaaaag ggttttgggt aagtaccaaa atcaaaacag
    18301 ttgtatttga ttcggcagat atgccttctg atgatctttt tctgaaatgc ttacagtctt
    18361 catataaggc ttattctgaa actgaacctg ccggtttaga taaacggcag ctgatgaaaa
    18421 cgttaattca aaaatgcgga ttcagttgtt gacgaaaaaa tggattctga actaacagaa
    18481 actgatttcg accatgtatg aggtaaaaaa tgtctttcgg ctatttgatt gcaacatcgc
    18541 aaccttgcga attgttgaca aaatctcgag gtgaaacttt ttctttaatt atggataaga
    18601 tggatttatg gatttatttc agattttgtg aaggaaatat ttatacaata aggaagaatg
    18661 aaactgaatc ttgccttaca gaaagaggag gtgaatggtt gaaacatatt tatgaattta
    18721 atcgggaaag ttttattttt tctgatgtgc ttctacaaaa gggagaaagc gaagagaatt
    18781 tttcagaaat tgtcttaaaa tcaataaaaa ataataaaat tttgacagtt gaggtacggt
    18841 caggcttgca ttttgactta agaaatattt atcgtattga gatgtaataa tgcaattgat
    18901 atgtgccgat tggacaggta taggaaattt tatgaaaacc ttcgaaaaaa catggtctgc
    18961 acaatatcgg gatatggaaa tttcagtacg gaatttttgg aatttgaaac ggacaggggc
    19021 ggaagtctat atcaacggca ggcgggttta tcataacgaa gccgggatgg cgtctgcttc
    19081 tttgcgttcg ctaatggggg aatacctgga atttgaagaa agcggtacga aaattaccgt
    19141 tgaaatcggc agcgcgtggc attttaatga aactataaga aatattaata cacatttaag
    19201 aggtacgaaa cttccaggtt ctaactggag agttcaagat aaggggcatt ttcatctttg
    19261 gaaaagataa taaattctaa ggtaagggag actaatttta atcaggcatg gcgtgttata
    19321 cgcgccatgc aaataaataa tatatctgat gaacaggtta tgattgtcaa aatgtcttgt
    19381 tatcttaaga aagatgaaat ttttaagaat atagaaagat atggatatca gtcatcagat
    19441 attaagaaaa ttaatcctcc ttttccatta aatattttac ctgttcccaa accttattat
    19501 ttttatcttt atgataccca tttgaatcaa atttatgatt tggcttggtc atgtaatgat
    19561 tttttaatgt ttgaaatatt tattttggat agggaaagca tcaaatatat agataaagat
    19621 aaacttttta aacctggaga aacactatat ttgaatttta aatctattaa aaatcaatgc
    19681 aaaaattatt tttttgcggt tttggttttg atgcccctta ctttaaaagc ggtgcgagtc
    19741 aagtggcaga ttataatttt gttcctgatg ccttttcttt attctttatt taatcaaatt
    19801 atttgaataa gcgtagggtg ggcacttgct gcccgcgtgt tctgtttcaa gtttattatt
    19861 gggtgaatat ttggaatttg aagaaaacgg tacaaagatt gctgcggaaa tcggcagtgc
    19921 atggcatttt ttgggcgcgg cttgtcggat ttcaataaat ggaaagtatt atgcgggtaa
    19981 tcggattgtt tggtttgcca aaaaagccga aagctaggaa taatttgaca agggcagcct
    20041 gaaagatttt caggctgcct tttatgtgga aaatatgatg aagttcaaat atgtttttct
    20101 gttggcgtgt gttgccgttt cttta
//
LOCUS       DAWTQS010000037        19312 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.037, whole genome shotgun sequence.
ACCESSION   DAWTQS010000037 DAWTQS010000000
VERSION     DAWTQS010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19312
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.037"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(91..1161)
                     /gene="lptG"
                     /locus_tag="WNA25_001297"
     CDS             complement(91..1161)
                     /gene="lptG"
                     /locus_tag="WNA25_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFA9824043.1"
                     /translation="MNLISRYIIRQMAVMAVCALLAFLALYSFFEILYETGNLGKGSY
                     GIWEMLGYTALKMPARAYELMPLAVLIGGLASLSQLAAGSELAVIKASGMSTKKLLLI
                     LSQFGFIFAIAAVALGEWVAPTLSQKAENIKAAAINGKISTGHTGLWLKEKNSIINVR
                     GMLPDHTLLGIKIWARNDKNELAEAVEADSAVLNSDGSWQLKNIRRSIMGTDKIETSA
                     AAEETWPIAVRRNLMDVLLVKPDQMSVGELTTYIRHLQNNSQNTQIYAIAWWRKLVYP
                     VAAWVMALVAFAFTPQTTRHGNMGLKLFGGICLGLLFHLAGRLFGFTSQLYGTPPFLA
                     GALPTIAFALLAVWLIRKQEKR"
     gene            complement(1158..2273)
                     /gene="lptF"
                     /locus_tag="WNA25_001298"
     CDS             complement(1158..2273)
                     /gene="lptF"
                     /locus_tag="WNA25_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFA9824044.1"
                     /translation="MIYQRNLIKELSFTAVGIFVVLLAVLVSTQAINLLGRAADGRVA
                     IDAVLALVGFWVIGMTPLLLVLTAFISTLTVLTRYWRDSEMSVWLSCGLALKQWIRPV
                     MQFAVPFAILIAVMQLWVIPWAELRSREYAEILKQKQELSLVEAGEFNNLGKRNGRVY
                     FVETFDTESGIMKNLFLREQDKNGGDNIIFAKEGNFSLNDNKRTLELRHGYRYSGTPG
                     RADYNQVSFQKLNLIISTTPKLIDPVSHRRTISTAQLIGSSNPQHQAELMWRISLTVS
                     VLLLCLLAVPLSYFNPRSGHTYNILIAIGLFLIYQNGLTLLFEAVEDGKIHFWLGLLP
                     MHIIMFVIAIVLLRVRSMPSQPFWQAVGKSLTLKGGK"
     gene            2262..2444
                     /locus_tag="WNA25_001299"
     CDS             2262..2444
                     /locus_tag="WNA25_001299"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824045.1"
                     /translation="MINHKIAVRGAVSGSAQADCPRKVSDGASKNGVQSCQPSSDGLL
                     SERPSENYRQPSQSGE"
     gene            2447..3853
                     /locus_tag="WNA25_001300"
     CDS             2447..3853
                     /locus_tag="WNA25_001300"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFA9824046.1"
                     /translation="MEFSTKTEILQEQQAGAQLFVCADKAPEHNTAAHALFSALEEGQ
                     NFSDTKIPTDNGLQAVAVVRLEKTDRAALNKAAAEAAKWAQNQETVNVDVHAFDEAQA
                     AAVAEAFAIAFGNAAYRFDRYKKEAKPAKFSQAVFHSAHEAAVKEALRVAEAQVYGQS
                     LCRDLGNAAPNECTPEFLARTAKAEAEKLGAHAKIIEKDYIKENMGSFWSVAKGSVED
                     PYLVELSYFGAADKEAAPVVLVGKGITFDTGGISLKPGLNMDEMKFDMCGAATVISTF
                     CAAVKLQLPINLIAIVATCENMPSGAANKPGDVVKSMKGLTIEVLNTDAEGRLILCDA
                     LTYAEQFKPKAVIDVATLTGACIVALGHDVSGVMGNNQDLIDSLLAASYNVDDKAWQL
                     PLFETYKDQLKSNFADIPNIGTPGAGTITAATFLSYFTEGYPWAHLDIAGTAWKSGAE
                     KGATGRPVPLLMNYLRNL"
     gene            3916..4356
                     /locus_tag="WNA25_001301"
     CDS             3916..4356
                     /locus_tag="WNA25_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFA9824047.1"
                     /translation="MPKVTFYTHVDQIPLFTCRLIARAIRDGGRILVWSDSFGRLQEL
                     DKMLWQYEAESFIPHEIWETEEAMPSDTSVLLACGGNLPRIPEGMAVLNLSDGFWNTA
                     SVLPARVLEIVGNSLEDLADARERFTAYRRSGFAIEHHGMEGKA"
     gene            4616..5434
                     /locus_tag="WNA25_001302"
     CDS             4616..5434
                     /locus_tag="WNA25_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFA9824048.1"
                     /translation="MNTIFKISALTLSAALALSACGKKEAAPASASEPAAASAAQGDT
                     SSIGGTMQQASYAMGVDIGRSLKQMKEQGAEIDLKVFTDAMQAVYDGKEIKMTEEQAQ
                     EVMMKFLQEQQAKAVEKHKADAKANKEKGEAFLKENAAKDGVKTTASGLQYKITKQGK
                     GKQPTKDDIVTVEYEGRLIDGTVFDSSKANGGPATFPLSQVIPGWTEGVRLLKEGGEA
                     TFYIPSNLAYREQGAGEKIGPNATLVFDVKLVKIGAPENAPAKQPDQVDIKKVN"
     gene            5541..6167
                     /locus_tag="WNA25_001303"
     CDS             5541..6167
                     /locus_tag="WNA25_001303"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFA9824049.1"
                     /translation="MKNIVILISGRGSNMQAIVNAAIPNVRIAAVLSNSETAAGLQWA
                     AERGIPTDSLNHKNFESRLAFDTAMMEKIDAYQPDLVVLAGFMRILTPEFCAHYEGRL
                     MNIHPSILPSFTGLHTHERALEAGCRVAGCTIHFVTAELDCGQIVSQGIVPILDGDTA
                     DDVAARVLAVEHKLYPKAVADFAAGRLIIEGNRVRNSENADAARFLTA"
     gene            6183..6854
                     /locus_tag="WNA25_001304"
     CDS             6183..6854
                     /locus_tag="WNA25_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HFA9824050.1"
                     /translation="MMKTFKNIFSAAILSAALPCAYAARLPQSAVLHYSGSYGIPATM
                     TFERSGNAYKIVSTIKVPLYNIRFESGGTVVGNTLHPAYYKDIRRGKLYAEAKFADGS
                     VTYGKAGESKTEQSPKAMDLFTLAWQLAANDAKLPPGLKITNGKKLYSVGGLNKAGTG
                     KYSIGGVETEVVKYRVRRGDDTVTYFFAPSLNNIPAQIGYTDDGKTYTLKLKSVQING
                     QAAKP"
     gene            6972..7394
                     /locus_tag="WNA25_001305"
     CDS             6972..7394
                     /locus_tag="WNA25_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFA9824051.1"
                     /translation="MQVTSKWIDGMCFVGTAEGGHSVVMEGSAAEGAAKRGPSPLEML
                     LLGVAGCSSIDVVMIAEKQRQKVTDCRATVTAKRADDAPRVFTEIHIHFKVIGHDLKE
                     SAIERAVQMSAEKYCSASIMLGKAAKITHSFEIAGAEK"
     gene            7917..8633
                     /locus_tag="WNA25_001306"
     CDS             7917..8633
                     /locus_tag="WNA25_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFA9824052.1"
                     /translation="MNFENDDIIHAPTTSSLILEERHDSELFRVYALILDGITDQVLL
                     PGKKLTESELCRQMVCSRNTVRGALSLLAHDKIVDLQPNRGAFVHVPDLKEMQDVFNA
                     RIEMETMILNILAGLPDLETRLKPLYAMIRCEEEASGRGDRVGWNRLSNAFHVELARL
                     VGNDVLFDIMNTLCARSSLIVAVAGVHREKKHAINTHTHSEHREILDLLLAGRRNRVV
                     KILRRHLGNCMERLEKTLED"
     gene            8782..9684
                     /locus_tag="WNA25_001307"
     CDS             8782..9684
                     /locus_tag="WNA25_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HFA9824053.1"
                     /translation="MSSLMTLFSVLVPMFAGFFIRVPKPYLPASDKVLSVLVYAVLLL
                     IGVSLSRVEDLGSRLGDMALTVLWLFVCTVGANLLALAVLGKLSPWRIGGKGKGVSVG
                     VSGSVRQLGCVLIGFVSGKLMCDIWMPSENAGMYCLMLLVFLIGVQLKSSGVSLRQVL
                     LNRRGIRLSVWFILSSLSGGLLFAASADGVSWTKGLAMASGFGWYSLSGLVMTEAYGA
                     VWGSIMLLNDLARELFALAFIPLLMKRFPDAAVGVGGATSMDFTLPVIQGAGGLEVVP
                     VAVSFGVVVNIAAPFLMVVFSTLG"
     gene            9727..10501
                     /locus_tag="WNA25_001308"
                     /pseudo
     CDS             9727..10501
                     /locus_tag="WNA25_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742658.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
     gene            10613..12301
                     /locus_tag="WNA25_001309"
     CDS             10613..12301
                     /locus_tag="WNA25_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HFA9824054.1"
                     /translation="MLNKDQFADNHFIRTIIEDDLKSGKHEAVQTRFPPEPNGYLHIG
                     HAKSICLNFGLAYIYDGLCNLRFDDTNPEKENDEYVNAIKEDVEWLGFHWAGEPRFAS
                     DYFDRLYDYAVGLIKDGKAYVDDLTPEEMREYRGTLTEAGKNSPYRDRSIEENLDLFT
                     RMKNGEFPDGSKTLRLKIDMAAGNINMRDPVIYRIRRAHHHNTGDKWCIYPMYDYTHC
                     ISDAIEGITHSLCTLEFEAHRPLYDWVLDNIPALHATRPRQYEFSRLELLYTITSKRK
                     LNQLVVEKHVSGWDDPRMPTISGMRRRGYTPEGVRLFAKRAGISKSENIVDMSVLEGA
                     IREELENSAPRLMAVLNPLKVTLTNFQAGKTQSRRAAFHPNHEEMGDREVPVSQTIYI
                     EADDFAENPPKGFKRLIPGGEVRLRHGYVIKCGEVVKDEAGNVVELKCSIDHDTLGKN
                     PEGRKVKGVIHWVSAEHAAEIKVRLYDRLFTVERPDAVRGEDGEYLPFTDFLNPESVK
                     EITAYAEPAAKDLPAESRWQFERIGYFVTDRQDHGKDMPVFNRTVTLKDSWQPK"
     gene            12393..13349
                     /gene="gshB"
                     /locus_tag="WNA25_001310"
     CDS             12393..13349
                     /gene="gshB"
                     /locus_tag="WNA25_001310"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFA9824055.1"
                     /translation="MKVLFIADPMASFKTYKDTTYAMMREMAKRGWRLFHTLSGELSV
                     NGGLVTAQASAFEFSGAKNDDDHEWFKAADKVQTALKAFDAVIMRTDPPFDMQYLYST
                     QLLTLAEQQGAKVFNSGRAMRDFNEKLAILNFSRFTAPTLVTTRSADVRAFLKEHGDI
                     IVKPLDGMGGMGIFRLTEKDPNIGSILETLMRFDSRTIMAQRYIPEIVHGDKRILIIG
                     GEVVPYALARIPQNGETRGNLAAGGRGVAQELDGRDREIAETLAPELKRSGILLAGLD
                     VIGSNLTEVNVTSPTGFQEIMKQKSFDVAAMFADAVAAWSVR"
     gene            13508..13891
                     /locus_tag="WNA25_001311"
     CDS             13508..13891
                     /locus_tag="WNA25_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFA9824056.1"
                     /translation="MGPSSYAAEKKGKSGIRRVINAFGYSIDGIAAAYRYEAAFRQVL
                     WLNALLVCAAFFWVSETAVRLPLIIASFVSVIVELFNTAVEAAVDHTSTEKHELAKRA
                     KDAGSAAQLVAMLMLAAVWLSALFG"
     gene            14023..14496
                     /locus_tag="WNA25_001312"
     CDS             14023..14496
                     /locus_tag="WNA25_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HFA9824057.1"
                     /translation="MKKLLAAVMMAGLAGAVSAAGVHVEDGWARTTVEGMKMGGAFMK
                     IHNDEAKQDFLLGGSSPVADRVEVHTHINDNGVMRMREVKGGVPLEAKSVTELKPGSY
                     HVMFMGLKKQLKEGDKIPVTLKFKNAKAQTVQLEVKTAPMPAMNHGHHHGEAHQH"
     gene            14621..15724
                     /gene="mnmA"
                     /locus_tag="WNA25_001313"
     CDS             14621..15724
                     /gene="mnmA"
                     /locus_tag="WNA25_001313"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFA9824058.1"
                     /translation="MNTTSNTSNIIVGLSGGVDSSVTAALLKQQGYQVRGVFMQNWEN
                     DDNDEYCSIKQDSFDAIAVADIVGIDIDIVNFAAQYKDKVFAYFLQEYSAGRTPNPDV
                     LCNAEIKFKCFLDYAVGQGADTIATGHYARKEVRNGVHYLLKGLDRNKDQSYFLYRLK
                     PFQLERAIFPLGGLEKPEVRRLAAEFNLPTAAKKDSTGICFIGERPFREFLQKYLPTD
                     NGKMVTPEGKTIGEHVGLMFYTLGQRKGLGIGGAGEPWFVAAKDLTKNELIVVQGHDH
                     PLLYTRSLVMNDLSFTLPERPKAGRYTCKTRYRMADAPCELCYLDDETAELVFDEPQW
                     AVTPGQSAVLYDVDICLGGGIIQTTDKPVIITR"
     gene            15795..17465
                     /locus_tag="WNA25_001314"
     CDS             15795..17465
                     /locus_tag="WNA25_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003740634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFA9824059.1"
                     /translation="MEKIWLESYEKGVSAEIDITQYNSVSDVFRQSVEKFARLPAFQN
                     MGKTLTYAETGKLATDFASYLQNVLKLPRGERVAIMMPNVLQYPIALFGILQAGLVAV
                     NTNPLYTPRELEHQLKDSGATAIIVLENFANTLELVLPRTQIKHVIVASVGEMFGLLK
                     GSLINFIIRKIKKMVPEYRIRETVSFQTALKEGAKHVFQPVALNREDTALLQYTGGTT
                     GVARGAVLSHGNICANMLQAKEWIKNQLREGKETVIAALPLYHIFALTVNLMIFANAG
                     SKIVLIANPRDMKGFIGELKKQRVNVFIGVNTLFNAMVNRPDFAEVDFSGLRLTLGGG
                     MATQKAVAEKWKKITGTPIVEAYGLTEASPGVCCNPLNIESYSGSIGLPVPSTEVELR
                     DANGKEVPVGQPGELWVKGPQVMQGYWNRPEETAKAIDACGFLETGDIAVMDEKGRLK
                     LVDRKKDLVVVSGFNVYPNEIEEFIAHHEKVMEVACIGVPNEKTGEALKVFVVKKDPS
                     LTKEELTAFCRTGLTAYKVPKDIEFRDELPKSNVGKILRRELRQSAGK"
     gene            17577..19211
                     /locus_tag="WNA25_001315"
     CDS             17577..19211
                     /locus_tag="WNA25_001315"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFA9824060.1"
                     /translation="MTKFIFVTGGVVSSLGKGIAAASIAAILESRGLNVTMLKLDPYI
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIDSTMTRRNSFSTGQVYENVIAKE
                     RRGDYLGGTVQVIPHITDEIKRRIHEGAAGYDVAIVEIGGTVGDIESLPFLEAIRQMR
                     SQLGRNNTLFAHLSYVPYIAAAGEIKTKPTQHTVKEMLSIGLQPDILICRMDRKMPAD
                     ERRKIALFCNVEERAIVGSYDVDSIYECPEMLHDQGIDNIITEQLQLNVQQADLTAWK
                     KIVHAVKNPKHTVKIAMVGKYVDLTESYKSLIEALKHAGIHTETDVQITFVDSESIEK
                     NKGDVSVLKDMDAILVPGGFGSRGVEGKIAAVRYARENNVPYLGICLGMQIALIEYAR
                     DVAGLKGANSTEFDLKCAAPVVALIDEWQTADGSVETRDESADLGGTMRLGAQEVELK
                     AGSLAVKIYGSGHIRERHRHRYEVNNNYVSALEQAGLVIGGVSAGRERLVETIELPNH
                     PWFFACQFHPEFTSNPRKGHPLFTAFVKAALNNKKA"
BASE COUNT         4737 a         5179 c         5233 g         4163 t
ORIGIN      
        1 aatccactat aatagggaac ggctatatgt ctgcgggaat gtgtcaatga aaacaaatgc
       61 cgtctgaaga gaggttcgga cggcatttgt tcaacgtttt tcctgtttgc gtatcagcca
      121 aacagcgagc aaggcgaagg ctatggtagg cagtgcgccg gcgaggaagg gtggggtgcc
      181 gtagagttgg ctggtaaacc cgaagagcct gccggcaagg tggaacagca atccgagaca
      241 gatgccgccg aagagtttta agcccatatt gccgtggcgc gtggtttgcg gcgtaaaggc
      301 gaaggcaacg agcgccatga cccatgcggc gacggggtaa acgagtttac gccaccatgc
      361 gatggcgtag atttgggtgt tttggctgtt gttttggagg tggcggatgt aggtggtcag
      421 ctcgccgacg gacatttggt cgggcttgac gagcaatacg tccatcaggt tgcgtctgac
      481 ggcaatcggc caagtttctt cggcggcggc ggatgtttcg attttgtctg tacccatgat
      541 gctgcggcgg atgtttttca actgccagct gccgtcgctg ttcaaaacgg cggaatcggc
      601 ttccactgcc tctgccaatt cgtttttatc gttgcgcgcc caaattttga tgcccaaaag
      661 cgtatggtcg ggcaacattc cgcgcacatt gataatgctg tttttttctt tcagccaaag
      721 gccggtatgg ccggtgctga ttttgccgtt aatggcggcg gctttgatgt tttcggcttt
      781 ttggctcagc gtgggcgcaa cccattcgcc gagcgcgacg gcggcaatag caaaaataaa
      841 accgaactga gacagaatca acagcagctt tttggtgctc atgccgctgg ctttgatgac
      901 ggccagttcg ctgccggcgg caagctggct gagagaggcc agtccgccga tgaggacggc
      961 gaggggcatg agttcgtagg cgcgggcggg cattttgagg gcggtgtagc ccagcatttc
     1021 ccatatgccg taactgcctt tgccgaggtt gccggtttcg tacaggattt caaaaaagct
     1081 gtacaaagcg aggaaggcaa ggagcgcgca aaccgccata accgccattt ggcggatgat
     1141 gtaacgtgaa atcaggttca ttttccgcct ttcaatgtca gacttttgcc aaccgcctgc
     1201 cagaagggct ggctgggcat actgcggacg cgcagaagta cgattgcgat gacgaacatg
     1261 atgatgtgca taggcagcag tccgagccaa aaatggattt tgccgtcttc cacggcttca
     1321 aaaagcaggg tcagcccgtt ttggtaaatt aaaaacaaac cgatggcaat caagatattg
     1381 taggtatgtc cgctgcgcgg gttgaaatag gaaagcggca cggcgagtag gcagagcagg
     1441 aggacgctga cggtcagcga gatgcgccac atcaattctg cctgatgttg cggattgctg
     1501 ctgccaatca gttgggcggt cgaaatggtg cggcggtggg aaacggggtc gataagtttg
     1561 ggcgtggtgc tgataatcag gttgagtttt tggaaggaaa cctgattgta gtcggcgcgt
     1621 ccgggcgtgc cgctgtaacg gtagccgtgg cgcaattcga gcgtgcgttt gttgtcgttc
     1681 agcgagaagt taccttcttt ggcgaagatg atgttgtcgc cgccgttttt gtcctgttcg
     1741 cgcaggaaca ggtttttcat gatgccggat tcggtgtcaa aggtttcgac gaaataaacc
     1801 ctgccgttgc gcttgcccaa gttattgaac tcgccggctt ccaccaaaga caattcctgc
     1861 ttctgcttca aaatttcggc atattcgcgg ctgcgcagtt ccgcccacgg tatcacccaa
     1921 agctgcatga cggcaatcag gatggcaaac ggcacggcaa actgcatgac ggggcgtatc
     1981 cactgtttca acgccaatcc gcaggatagc cagaccgaca tttcgctgtc gcgccagtag
     2041 cgggtcaata cggtcagcgt gctgatgaat gcggtcaaca ccagcaaaag cggggtcata
     2101 ccgatgaccc agaagccgac taaggccaac acggcatcga tggcgacacg cccgtcagct
     2161 gcgcggccaa gcaggttgat cgcctgcgtg gacaccaaca ccgccaagag gacgacgaaa
     2221 atgccgacgg cggtaaaaga gagttctttg atgaggtttc tttgataaat cataaaatcg
     2281 cggtcagggg ggcggtaagc ggttcggcgc aggcggattg tccgcgcaag gtttcagacg
     2341 gtgcttcaaa aaatggggta caatcgtgcc agccgtcttc agacggtctt ttgagcgaaa
     2401 ggccgtctga aaactaccga caaccgtctc aatcaggaga ataaacgtgg aatttagcac
     2461 aaaaaccgaa atcttgcagg aacagcaggc aggcgcgcag ttatttgtct gtgccgacaa
     2521 agcacccgag cacaacaccg ccgcacatgc gctcttctct gctttggaag agggtcagaa
     2581 tttttccgac accaaaatcc cgacggacaa cggtttgcag gcagtcgccg tcgtccgcct
     2641 cgaaaaaacc gaccgcgccg cgctgaacaa agccgccgcc gaagccgcca aatgggcgca
     2701 aaatcaagaa acggtcaatg tggacgttca cgcctttgat gaggcgcaag cagcagccgt
     2761 tgccgaagct tttgccatcg cgttcggcaa cgctgcatat cgtttcgacc gctacaaaaa
     2821 agaagccaag cccgccaaat tttcgcaagc cgtgttccac agcgcacacg aagccgccgt
     2881 caaagaagcc ctgcgcgttg ccgaagcaca agtttacgga caaagcctct gccgcgactt
     2941 aggcaatgcc gcaccgaacg aatgcacgcc tgagttcctc gcgcgtaccg ccaaagccga
     3001 agccgagaaa ctgggcgcgc acgccaaaat catcgaaaaa gactacatca aagaaaacat
     3061 gggttcgttt tggtcggttg ccaaaggcag cgtcgaagac ccatatttgg ttgaactgag
     3121 ctatttcggt gcggccgaca aagaagccgc gcctgtggta ttggtcggca aaggcattac
     3181 cttcgacacc ggcggcattt ccctcaaacc cggcctgaac atggacgaaa tgaagtttga
     3241 catgtgcggc gcggcaaccg tcatcagcac cttctgcgcc gccgtcaaac tgcaactgcc
     3301 gatcaatctg attgccatcg tcgccacttg tgaaaacatg ccttccggcg cggcaaacaa
     3361 accgggcgat gttgtgaaaa gtatgaaagg gctgacgatt gaagtgttga acaccgatgc
     3421 cgaaggccgt ctgattttgt gcgacgcgct cacttacgcc gagcagttca aacccaaagc
     3481 cgtcatcgat gtcgccaccc tgaccggcgc gtgcatcgtc gccttggggc acgatgtcag
     3541 cggcgtgatg ggcaataatc aggatttgat cgacagcctg cttgccgctt cctacaacgt
     3601 ggacgacaaa gcgtggcaac tgccgctctt tgaaacctac aaagaccagc tcaaatccaa
     3661 ctttgccgac atccccaata tcggcacgcc cggcgcgggc acgattaccg ccgcaacatt
     3721 cctgtcctat ttcaccgaag gctacccgtg ggcgcacctt gacatcgcgg gtacggcgtg
     3781 gaaatccgga gcggaaaaag gtgcgaccgg ccgccccgta ccgctgctga tgaactatct
     3841 gcggaatctt taattttgcc acaatgccgt ctgaagcttt tcagacggca tcatcctgtc
     3901 aaatgctcaa acaatatgcc gaaagttacc ttttataccc atgttgacca aatccccctt
     3961 tttacctgtc ggttgattgc gcgcgctatc cgagacggcg gcaggatact ggtgtggtcc
     4021 gactcgttcg ggcggcttca ggaattggac aaaatgcttt ggcaatacga ggccgagagt
     4081 tttattccgc acgaaatttg ggaaacggaa gaagccatgc cgtctgacac atccgtcctg
     4141 cttgcctgcg gcggcaatct gccccgaatt cccgaaggca tggccgtttt gaacctgtcc
     4201 gacggttttt ggaacaccgc ttcggtcttg cccgcgcgcg ttttggaaat cgtcggcaac
     4261 agcctggaag atctcgccga cgcgcgcgaa cgctttaccg cctaccgccg aagcggtttt
     4321 gccatcgaac atcacggcat ggagggcaag gcatgaaatg ccgtctgaaa tgccgcattt
     4381 acaaaattta aacaggcttg attctatttg attaaaattt ccttatcggt tgaaccccgc
     4441 cacttggaca tctgtccttc ggggcggtag aatcaaacct tattttggaa ggttcaatcc
     4501 cttccaaaac agggcaacac acagattgac gctttatgtg ccatcctgtg tgttgaaaca
     4561 ttcaaactcg gctataatcc cgtttttccg actttatcga cagcgaagat ccatcatgaa
     4621 caccattttc aaaatcagcg cactgaccct ttccgccgct ttggcgcttt ccgcctgcgg
     4681 caaaaaagaa gccgcccccg catctgcatc cgaacctgcc gccgcttctg ccgcgcaggg
     4741 cgacacctct tcaatcggcg gcacgatgca gcaggcaagc tatgcgatgg gcgtggacat
     4801 cggacgctcc ctgaaacaaa tgaaggaaca gggcgcggaa atcgatttga aagtctttac
     4861 cgatgccatg caggcagtgt atgacggcaa agaaatcaaa atgaccgaag agcaggccca
     4921 ggaagtgatg atgaaattcc tgcaggagca gcaggctaaa gccgtagaaa aacacaaggc
     4981 ggatgcgaag gccaacaaag aaaaaggcga agccttcctg aaggaaaatg ccgccaaaga
     5041 cggcgtgaag accactgctt ccggtctgca gtacaaaatc accaaacagg gtaaaggcaa
     5101 acagccgaca aaagacgaca tcgttaccgt ggaatacgaa ggccgcctga ttgacggtac
     5161 cgtattcgac agcagcaaag ccaacggcgg cccggccacc ttccctttga gccaagtgat
     5221 tccgggttgg accgaaggcg tacggcttct gaaagaaggc ggcgaagcca cgttctacat
     5281 cccgtccaac cttgcctacc gcgaacaggg tgcgggcgaa aaaatcggtc cgaacgccac
     5341 tttggtattt gacgtgaaac tggtcaaaat cggcgcaccc gaaaacgcgc ccgccaagca
     5401 gccggatcaa gtcgacatca aaaaagtaaa ttaagtccga atccatgccc gaaacaggtt
     5461 ttcgggcatt tttacggcaa atgccgtctg aagccaccaa acagcggttc agacggcatc
     5521 cctttcaaaa gcgcaccatt atgaaaaaca tcgtcatcct gatttccgga cgcggcagca
     5581 atatgcaggc aatcgtcaat gccgccattc ccaacgtccg cattgccgcc gtgttgagca
     5641 acagcgaaac ggctgccggt ttgcaatggg cggccgaacg cggcatcccg accgacagcc
     5701 tgaatcataa aaactttgaa tcacgacttg ccttcgatac ggccatgatg gaaaaaatcg
     5761 acgcatatca acccgatttg gtggttttgg caggttttat gcggattctg acccctgagt
     5821 tctgcgccca ttacgaaggc aggctgatga acatccatcc gtccatcctg ccctcgttca
     5881 ccgggcttca tacgcacgaa cgcgctttgg aagcaggttg ccgcgttgcc ggctgtacga
     5941 ttcatttcgt tacggccgag ctggattgcg gccagattgt atcgcaaggg attgtgccga
     6001 tacttgacgg cgatacggca gacgatgttg ccgcccgggt tttggctgtc gaacacaaac
     6061 tctatccgaa agccgttgcc gattttgccg ccggccgcct gattatcgag ggaaaccgtg
     6121 tcagaaattc ggaaaacgcc gatgccgccc gtttcctgac ggcgtaaacc gggcgggagc
     6181 aaatgatgaa gacttttaaa aatatatttt ccgccgccat tttgtccgcc gccctgccgt
     6241 gcgcgtatgc ggcaaggcta ccccaatccg ccgtgctgca ctattccggc agctacggca
     6301 ttcccgccac gatgacattt gaacgcagcg gcaatgctta caaaatcgtt tcgacgatta
     6361 aagtgccgct atacaatatc cgtttcgagt ccggcggtac ggttgtcggc aataccctgc
     6421 accctgccta ctataaagac atacgcaggg gcaaactgta tgcggaagcc aaattcgccg
     6481 acggcagcgt aacctacggc aaagcgggcg agagcaaaac cgagcaaagc cccaaggcta
     6541 tggatttgtt cacgcttgcc tggcagttgg cggcaaatga cgcgaaactc cccccgggtc
     6601 tgaaaatcac caacggcaaa aaactttatt ccgtcggcgg cctgaataag gcgggtacgg
     6661 gaaaatacag cataggcggc gtggaaaccg aagtcgtcaa atatcgggtg cggcgcggcg
     6721 acgatacggt aacgtatttc ttcgcaccgt ccctgaacaa tattccggca caaatcggct
     6781 ataccgacga cggcaaaacc tatacgctga agctcaaatc ggtgcagatc aacggacagg
     6841 ccgccaaacc gtaaatcggt tttcaggcgg ttttttgccg ttttgtccat atttctgtat
     6901 aagccgcttg aaattgttta caataacgcc atatgcaaag cggttttaac gctattttcg
     6961 ggaatgacac catgcaggtt acatcaaaat ggatagacgg aatgtgtttt gtcggcacgg
     7021 cggaaggcgg gcacagcgtc gttatggagg gatcggcggc agaaggcgcg gccaagcgcg
     7081 ggcccagccc tttggaaatg ctgctgttgg gggtggcggg ctgttcgagc atcgatgtgg
     7141 tgatgattgc cgaaaaacag cgtcagaaag tgactgactg ccgtgcgacg gttacggcga
     7201 aacgggcgga cgatgcgccg cgcgtgttta ccgaaatcca catccatttc aaggtaatcg
     7261 ggcatgattt gaaagaatcg gccattgagc gcgccgttca gatgtctgcc gaaaaatact
     7321 gttcggcttc gattatgttg ggcaaagcgg caaagattac ccacagtttt gaaattgccg
     7381 gggcggagaa ataggcatcg ggcggcatta tggggaaacg gctgatgccg tctgaagggg
     7441 aaaggtgcgg caggcaatcc ggaggcgcat aaaaaagcag atatattcgg actgcacctc
     7501 ccgaatatat ctgcctgctg tttcctcttt attcagcctt tataatactt ggacttgtcg
     7561 gggtattgtg caggcttgat tccggattgt caacaatttt cggtcaaatt ttaaatgccg
     7621 cgttttaaaa tgatgcccgc ctgattttgc gggcgggcgg aaggcgggaa tcccgttatt
     7681 ttaagccata tattaattta ttgaattaaa ataaatttgt gcaatatagt ggattaacaa
     7741 aaaccagtac ggcattacct cgccttgccg tactattttt gtactgtctg cggcttcgtc
     7801 gccttgtact gatttaaatt taatccacta tactttaatt tctcctacac gatttgaaac
     7861 acagccgtct taaaacatct taaaaaattt gaaagcgtaa aaatacggaa aacatcatga
     7921 actttgaaaa cgacgacatt atccatgcgc cgaccacgtc ttccctgatt ctcgaagagc
     7981 ggcacgattc ggagctgttc cgtgtttacg ccctgatttt ggacggcatt accgatcagg
     8041 tgctgctgcc cggcaaaaag ctgaccgaat ccgaactttg ccgtcagatg gtgtgttcgc
     8101 gcaacaccgt ccgcggcgcg ctgtcgcttt tggcgcacga caagattgtc gatttgcaac
     8161 ccaacagggg cgcgttcgtc cacgttcccg atttgaaaga aatgcaggat gtgttcaatg
     8221 cgcgcatcga aatggagacg atgattttga acatcctcgc cggcctgccg gatttggaaa
     8281 cgcgcctcaa gccgctttat gcgatgataa ggtgcgaaga agaggcctcc ggcaggggcg
     8341 accgcgtcgg ctggaaccgc ctgtccaatg ccttccacgt cgaactggcg cgcctggtgg
     8401 gcaacgatgt gctgttcgac attatgaaca cgctgtgcgc gcggtcttcc ctgattgtcg
     8461 ccgtggcggg cgtgcatcgc gagaaaaaac acgccatcaa tacgcatacg cattccgaac
     8521 accgcgaaat cctcgacctg ctgctggcgg gcaggcgcaa ccgcgtggtc aaaatcctgc
     8581 gccgccattt gggcaactgt atggagcgtt tggaaaagac tttggaagat tgaatgccgg
     8641 agcggataag ccgcctgaac cttcaggcgg ttttttaatg gcgaacctga tgccgtctga
     8701 aatatggatg cgggtatctg caattttcag acggcaattt ttaagccgca catatcatgc
     8761 ggcaataaag gagggtaggg gatgagcagc ctgatgacgt tgttttcggt attggtaccg
     8821 atgtttgccg gattttttat ccgtgttccc aagccttacc tgcccgcttc ggacaaggtg
     8881 ctgtcggttt tggtgtatgc cgtgctgctg ctgatcggcg tatcgttgtc gcgcgtggag
     8941 gatttgggtt cgcggttggg cgatatggcg ttgacggttc tgtggctgtt tgtttgtacg
     9001 gtaggggcga acctgcttgc cttggcagtg ttgggaaagt tgtccccgtg gcggataggg
     9061 ggaaaaggga agggcgtttc ggtcggcgtg tcgggcagtg tgaggcagct cggatgcgta
     9121 ctgatcggtt ttgtgtccgg caaattgatg tgcgatattt ggatgccgtc tgaaaacgcg
     9181 ggtatgtact gcctgatgct gctggtgttc ctcatcggcg tacagctcaa aagtagcggc
     9241 gtatcgttgc ggcaggtttt gcttaaccgg cggggcatcc ggctgtcggt ttggtttata
     9301 ttgtcatctc tttcaggcgg gctgctgttt gccgcatcgg cagatggtgt gtcgtggacg
     9361 aaaggtttgg cgatggcttc cggcttcggt tggtattccc tctcgggttt ggtaatgacc
     9421 gaggcttacg gggcggtatg gggcagcatc atgctgctga acgatttggc acgagagctg
     9481 tttgcactgg catttattcc gctgctgatg aagcgttttc cggatgcggc ggtgggggtc
     9541 ggcggcgcga ccagtatgga tttcacattg cccgtaattc agggtgcggg cggtttggaa
     9601 gtcgtgccgg tagcggtcag cttcggcgtg gtggtcaata tcgccgcccc gtttctgatg
     9661 gtggtgtttt ccacgctggg ctgaacgcgg taaaatcggc atcccgatgc aaggaagcag
     9721 aaaacgatga aaccgaaaat ccaaaggcat ggagagattt taagccttgt ccgccggcat
     9781 cagtttatgt cggtggacga gcttgccgcc gcattggacg ttaccccgca gacgatacgt
     9841 tgcgacatcc gcgagttgga ggagggcggc agcctgaaac gccatcacgg cggcgcatct
     9901 tcgggcggaa acttgccgga gggtctgccc gccgaccgcc aaacccggtg tcaaaacgaa
     9961 aaaaacgcca ttgcccggct gattgcggaa cacatccccg acggttcgtc gctgttcgtc
    10021 agtatcggta cgaccatgga agccgcggca tcagagctgg tgaagcggcg cagcagcctg
    10081 cggattatta ccaacaatat ccacgtcgcc tccgtcgttt cggcacgtac ggattacacg
    10141 gtcatcatca catccggcgt cgtccgccct ttggacggcg gtattaccgg cgtggcgacc
    10201 gtcgacttta tcaaccagtt caaagtcgat tatgccgtga tgagcacgca cggcgtggaa
    10261 agcgacggtt cgcttttgga ttacgattac aaggaagtca gcgtcatgca ggcgatgatt
    10321 gccaacgcgc gcgtccgttt tctcggcgtg gatcacagca aattccgcag caacgcgctg
    10381 gtcaggctcg gcggcattac cggcgtttga caaagtattt accgacaggt tgcctgatac
    10441 cgcgatgcag aagatgctga aagaggcggg ggtggaatgc ctgattgccg atgccgtctg
    10501 aacgctatgt caaagcgcgc aagtcgggta caataaacac atcatcaaac cgcttcagac
    10561 ggcatacgga acctccccaa tgccgtctga agccatctgt ttaaagaaaa ccatgctcaa
    10621 taaagaccaa ttcgcggaca accatttcat ccgcaccatc atcgaagacg acctcaaaag
    10681 cggcaaacac gaagccgtcc aaacccgttt tccgcccgaa cccaacggct atttgcatat
    10741 cggacacgcc aaatccatct gcctgaactt cggtttggcg tatatttacg acggtttgtg
    10801 caacctgcgt ttcgacgaca ccaacccgga aaaagaaaac gacgaatacg tcaacgccat
    10861 caaagaagac gtcgagtggc tcggtttcca ttgggcgggc gagccgcgtt tcgcttccga
    10921 ctatttcgac cggctttatg actacgccgt cggtttgatt aaagacggaa aagcgtatgt
    10981 cgatgatttg acacccgaag aaatgcgaga ataccgcggt acgctgaccg aagcgggtaa
    11041 aaacagccct taccgcgacc gcagtatcga agaaaacctc gacctgttca cgcgtatgaa
    11101 aaacggcgag ttccccgacg gcagcaaaac cctgcgcctg aaaatcgaca tggcggcagg
    11161 caacatcaat atgcgcgatc ccgtcatcta ccgcatccgc cgcgcccatc accacaacac
    11221 cggcgacaaa tggtgcatct acccgatgta cgattacacg cattgcattt ccgatgccat
    11281 cgaaggcatc acgcattcct tgtgcacgct cgaatttgaa gcgcaccgtc cgctttacga
    11341 ctgggtgttg gacaacatcc ccgcgctgca cgccacccgt ccgcgccaat acgagttttc
    11401 ccgtttggag cttttgtaca ccattacctc caaacggaaa ttgaatcagt tggttgtgga
    11461 aaaacacgtt tccggctggg atgatccgcg tatgccgacc atttccggta tgcgccgccg
    11521 cggctacacg cccgaagggg tgcgcctgtt tgccaaacgc gccggtattt ccaaatctga
    11581 aaacatcgtc gacatgagcg tgttggaggg tgcgattcgc gaagagctgg aaaactccgc
    11641 cccgcgcctg atggcggttt tgaacccgct caaagtgacc ctgaccaact ttcaagccgg
    11701 caaaacccaa agccgccgtg ccgcgttcca tccgaaccac gaagaaatgg gcgatcgcga
    11761 agtacctgtt tcacaaacca tctacatcga agccgacgac tttgccgaaa atccgcccaa
    11821 aggcttcaag cgtctgattc ccggcggcga agtacgcttg cgccacggct atgtcatcaa
    11881 gtgcggtgaa gtcgtcaaag acgaagcagg caatgtggtt gaactcaaat gcagcatcga
    11941 ccacgacacc ttgggcaaaa atccagaagg ccgcaaagtt aaaggcgtaa ttcactgggt
    12001 ttccgccgaa cacgccgccg aaatcaaagt ccgtctgtac gaccgcctct ttaccgtcga
    12061 gcgtcccgat gccgtgcgcg gcgaagacgg cgaatacctg ccgtttaccg atttcctcaa
    12121 tccggaatcc gttaaggaaa tcactgctta cgccgaacct gccgcgaaag atttgccggc
    12181 ggaaagccgt tggcagttcg agcgcatcgg ctattttgtg accgaccgcc aagaccacgg
    12241 caaagacatg ccggtgttta accgcacggt gacgttgaaa gattcttggc agcctaagta
    12301 aaccccatcc ttgccgtctg aatattgttc gggcggcatt tctcctttac ccgcgaaatg
    12361 cggcacactc ggcacaccgg aaaggaaata tgatgaaagt cctctttatc gccgacccga
    12421 tggcaagttt caaaacctac aaagacacca cctacgcgat gatgcgggaa atggcaaaac
    12481 gcggctggcg gctgtttcat accttgagcg gggaattgtc tgtaaacggc ggtttggtaa
    12541 cggcacaggc atcggcattt gaattttcgg gtgcaaaaaa cgatgatgac catgaatggt
    12601 ttaaagcggc ggacaaagtt cagacggcat taaaagcatt tgatgccgtg attatgcgta
    12661 ccgatccgcc gttcgatatg caataccttt actccaccca attactgacg ctggcggaac
    12721 agcagggcgc gaaagtgttc aacagcggac gggcgatgcg cgactttaac gaaaaactgg
    12781 cgattttgaa tttcagccgc tttaccgcgc ccacgctggt aacgacccgt tccgccgatg
    12841 tccgcgcatt tttgaaagaa cacggcgaca tcatcgtcaa accgctcgac ggcatgggcg
    12901 gcatgggcat cttccgcctg accgaaaaag accccaacat cggcagcatc ctcgaaaccc
    12961 tgatgcggtt tgattcccgc accattatgg cgcaacgcta cattcccgaa atcgtacacg
    13021 gtgacaaacg catcttgatt atcggcggcg aagtcgtccc ctatgctttg gcgcgtatcc
    13081 cgcaaaacgg cgaaacacgc ggcaatctgg cggcaggcgg gcgcggtgtg gcgcaggaat
    13141 tggacggacg cgaccgggaa attgcagaga ctctggctcc cgagcttaaa cggagcggca
    13201 tcctgctggc cggtttggac gttatcggca gcaacctgac cgaagtcaac gtaaccagcc
    13261 cgaccggatt ccaagaaatt atgaaacaaa aaagtttcga cgtggcggca atgtttgccg
    13321 atgccgttgc cgcgtggtcg gtacgttaaa ccgatgccgt ctgaaaggct tttgcttcgt
    13381 aaccgcttgg tttgttcggg caggcagggt tttccctcag ccggcaggga tgcgcttaat
    13441 gccgtacgga cacccctccg ccccgttttc agacggcata tattgaggac attttgaaag
    13501 gataccgatg ggaccttcct cctacgcggc agaaaaaaaa ggcaaaagcg gcatcaggcg
    13561 cgtcatcaac gcattcggct attcgataga cggcatcgcc gccgcctacc gttacgaagc
    13621 ggcattccgt caggttttgt ggctgaacgc gctgctggtg tgcgcggcat ttttttgggt
    13681 ttccgaaacc gccgtccgcc tgccgttgat tatcgcgtct tttgtgtcgg tcattgtcga
    13741 actgttcaac accgccgtcg aagccgccgt cgatcatact tcgaccgaaa aacacgagct
    13801 ggccaaacgc gccaaagatg caggttctgc cgcacaactg gttgccatgc tgatgttggc
    13861 ggcggtttgg ctgtccgccc tgttcgggta aaacgcttgc agcaggattt gtaatccttt
    13921 aggattagta tttattattc acttaatcta catcaaattt ccgagcagta tttgtatgta
    13981 agattaagca cattccccgt ctgatattaa aggagcagga agatgaaaaa attattggca
    14041 gccgtgatga tggcaggttt ggcaggcgcg gtttccgccg ccggagtcca tgtcgaggac
    14101 ggctgggcgc gcaccactgt cgaaggtatg aaaatgggcg gcgcgttcat gaaaatccac
    14161 aacgacgaag ccaaacaaga ctttttgctc ggcggaagca gccccgttgc cgaccgcgtc
    14221 gaagtgcata cccacatcaa cgacaacggc gtgatgcgta tgcgcgaagt caaaggcggc
    14281 gtgcctttgg aggcgaaatc cgttaccgaa ctcaaacccg gcagctatca cgtgatgttt
    14341 atgggtttga aaaaacaact gaaagagggc gacaagattc ccgttaccct gaaatttaaa
    14401 aacgccaaag cgcaaaccgt ccaactggaa gtcaaaaccg cgccgatgcc ggcaatgaac
    14461 cacggtcatc accacggcga agcgcatcag cactaatctg ctggaaatat ttgaaatgcc
    14521 gtctgaaaaa gcccgggctt tcagacggca tttttatgcc cgcctttaaa atgtgttaaa
    14581 atccgccttt aaaaaccgcc gtttccaagc catcctgcgt atgaatacga catcaaacac
    14641 ttccaatatc atcgtcgggc tttccggcgg tgtcgattct tccgtaaccg ccgccctgct
    14701 caagcagcag ggttatcaag tgcgcggtgt gttcatgcag aactgggaaa acgacgacaa
    14761 cgacgaatat tgcagcatca aacaggattc gttcgatgcc atcgccgttg ccgatattgt
    14821 cggcatcgac atcgacatcg ttaatttcgc cgcgcaatat aaagacaaag tttttgctta
    14881 ttttcttcag gaatacagtg cggggcgcac gccgaatccg gatgtgttgt gcaatgccga
    14941 aatcaaattc aaatgctttt tggactacgc cgtagggcag ggcgcggata ccattgccac
    15001 cggacactat gcgcgcaaag aagtccgcaa cggcgtgcat tacctgctca aaggtttgga
    15061 tcgaaacaaa gaccaaagct attttctcta ccgcctcaag cctttccaac tcgaacgcgc
    15121 gatttttccg ttgggcggtt tggaaaaacc cgaagtgcgc cgccttgccg ccgaatttaa
    15181 tttgccgact gccgctaaaa aagacagtac cggcatctgt ttcatcggcg agcgtccgtt
    15241 ccgcgagttt ctgcagaaat acttgccgac cgacaacggc aaaatggtga cgcccgaagg
    15301 gaaaaccatc ggcgaacacg tcgggctgat gttttacaca ttgggtcagc gcaaaggatt
    15361 gggcatcggc ggcgcgggcg aaccgtggtt tgttgcggct aaagatttga cgaaaaacga
    15421 actcatcgtc gtacaaggac acgaccatcc gctgctctat acccgcagcc ttgtgatgaa
    15481 cgatttgagt ttcacgctgc ccgaacgtcc gaaggcagga cgctatacct gcaaaacgcg
    15541 ttaccgtatg gcggacgcgc cttgcgaatt gtgctatttg gatgatgaaa ccgccgagct
    15601 ggtgtttgac gaaccgcaat gggcggttac gccgggtcag tccgccgtgc tgtacgacgt
    15661 cgacatctgt ttgggcggcg gcatcatcca aacgaccgac aaacccgtca tcatcacgcg
    15721 ataaaggtaa tgccgtctga aacggttttc agacggcatt gttccgctca attccacttt
    15781 aaagaccgat acctatggaa aaaatctggt tagaaagcta cgagaagggc gtcagtgccg
    15841 aaatcgacat cacgcaatac aattccgtca gcgacgtatt ccgccaaagc gtggaaaaat
    15901 ttgcccgtct gcccgctttt caaaatatgg gcaaaacgct cacttatgcc gaaaccggca
    15961 aactggcgac cgatttcgcc tcttatctgc aaaacgtcct caagctgccg cgcggcgaac
    16021 gtgttgccat tatgatgccg aacgtattgc aatatccgat tgcccttttc ggtattttgc
    16081 aggcaggttt ggtggcggtg aacaccaatc cgctctatac gccgcgcgag ttggagcatc
    16141 agctgaaaga cagcggtgcg accgccatca tcgttttgga aaatttcgcc aacacgctgg
    16201 agctggtgct gccgcgcacg cagatcaaac acgtcatcgt cgcctccgtc ggcgaaatgt
    16261 tcgggctgct taaaggttcg ctgatcaatt tcatcatccg aaaaatcaag aaaatggttc
    16321 ccgaataccg tattcgggaa accgtttcct ttcagacggc attgaaagag ggggcgaagc
    16381 acgttttcca acctgtcgca ttaaaccgcg aagataccgc attgttgcaa tacacgggcg
    16441 gcacgacagg cgttgccaga ggcgcggtgc tgagccacgg caacatctgc gccaatatgc
    16501 ttcaggcaaa agaatggatt aaaaaccaat tgcgcgaggg aaaagaaacc gttatcgccg
    16561 ccctgccgct gtaccacatc ttcgccctga cagtgaatct gatgattttt gccaatgccg
    16621 gctcgaaaat tgtcctgatt gccaacccgc gcgatatgaa aggctttatc ggcgaactga
    16681 aaaagcagcg ggttaacgta tttatcggcg tgaacacgct gtttaacgcg atggtcaacc
    16741 ggcccgattt cgccgaagtc gatttttcag gattgcggct gactttgggc ggcggtatgg
    16801 cgacccaaaa agccgttgcc gaaaaatgga aaaaaatcac cggcacgccc atcgtcgaag
    16861 cctacggttt gaccgaagcc agccccggcg tgtgctgcaa ccccttaaac atcgaatcat
    16921 acagcggcag catcggtttg cccgtcccgt ccaccgaagt cgaactgcgc gacgcaaacg
    16981 gcaaagaagt ccccgttggg cagccgggcg aattgtgggt aaaaggcccg caagtgatgc
    17041 aaggctactg gaaccgcccc gaagaaaccg ccaaagccat agacgcgtgc ggctttttgg
    17101 aaaccggcga tattgccgtg atggacgaaa aaggccgtct gaagctggtc gatcgcaaaa
    17161 aagacctcgt cgtcgtttcc ggattcaatg tttatccgaa cgaaatcgag gaattcatcg
    17221 cgcaccacga aaaagttatg gaagttgcgt gtatcggcgt tccgaacgaa aaaaccggcg
    17281 aggcactcaa agtgttcgtc gtcaaaaaag acccgtcttt gaccaaagaa gaactcaccg
    17341 ctttctgccg taccggtctg accgcataca aagtgccgaa agacatcgaa ttccgcgacg
    17401 agttgcccaa gtccaatgtc ggcaaaatcc tgcgccgcga gttgcgccaa agtgccggga
    17461 aataaagaaa agataccgtc tgaaaacagc cgtccaccgt tcagacggca tccgtccgtt
    17521 tgccagaacc gcgcgcttca cgttaaaatc acgcattcca acatgggtat tccatcatga
    17581 ccaaattcat tttcgtaacc ggcggcgttg tctcctcact gggtaaaggt atcgccgccg
    17641 cttctattgc cgccatcctc gaatcgcgcg gcttgaacgt taccatgctc aagcttgatc
    17701 cttatatcaa cgtcgatccc ggcacgatga gcccgttcca acacggcgaa gtgttcgtaa
    17761 ccgacgacgg cgcggaaacc gacctcgact tgggacacta cgaacgtttc atcgattcca
    17821 cgatgacccg ccgcaacagc ttcagcacgg gtcaggtgta cgaaaacgtt atcgccaaag
    17881 aacgccgcgg cgactatctc ggcggtacgg ttcaagtcat cccgcacatt accgacgaaa
    17941 tcaaacgacg catccacgaa ggcgcggcgg gttacgatgt ggcgattgtc gaaatcggcg
    18001 gtacggtcgg cgacatcgaa tcgctgccgt ttttggaagc catccgccag atgcgaagcc
    18061 agttgggacg caacaacacc ctgttcgccc acttgagcta cgttccctac atcgccgccg
    18121 caggcgaaat caaaaccaag ccgacccagc acaccgtgaa agaaatgttg agcatcggct
    18181 tgcaacccga catcctgatt tgccgtatgg acaggaaaat gccggcagac gaacgccgca
    18241 aaatcgcctt gttctgcaac gtggaagagc gcgcgattgt cggcagctac gatgtggaca
    18301 gcatctacga atgccccgaa atgctgcacg accaaggcat cgacaacatc attaccgagc
    18361 agttgcagct taacgtgcag caggcggatt tgaccgcgtg gaaaaaaatc gtccacgccg
    18421 tcaaaaaccc gaaacacacc gtcaaaatcg cgatggtcgg caaatacgtt gatttgaccg
    18481 aatcctacaa atcattgatt gaagccttga aacacgcggg catccatacc gaaaccgatg
    18541 tgcagattac cttcgttgac agcgaaagca tcgagaaaaa caagggcgac gtttccgtac
    18601 tcaaagatat ggatgccatc ctcgttcccg gcggcttcgg ttcgcgcggc gtggaaggca
    18661 aaatcgccgc cgtgcgctac gcccgtgaaa acaacgtgcc atacttgggc atctgcctcg
    18721 gtatgcagat tgcgctgatt gaatacgccc gcgacgtggc aggtttgaaa ggtgcgaatt
    18781 ccactgagtt tgacttgaaa tgcgctgccc ccgtcgtcgc cctgattgac gaatggcaaa
    18841 ccgccgacgg cagcgtcgaa acccgtgacg aatccgccga tttgggcggc acgatgcgtt
    18901 tgggcgcgca agaagtcgaa ttgaaagcag gcagcctcgc cgtcaaaatc tacggcagcg
    18961 gacacattcg cgaacgccac cgccaccgct acgaagtcaa caacaactac gtttccgcgc
    19021 tggaacaggc aggtttggtc atcggcggcg tatccgccgg acgcgaacgc ttggtcgaaa
    19081 ccatcgaact gccgaaccat ccttggttct tcgcctgcca gttccatccc gagttcacgt
    19141 ccaacccgcg caaagggcat cctttgttca ccgcgtttgt caaagccgcg ttgaacaata
    19201 aaaaagcctg ataaagcgtt acttgatgat caaaatgccg tctgaaagcc tgacaaagac
    19261 tttcagacgg cattttcgca aatcggggat cataaaagcc atcaatttaa tt
//
LOCUS       DAWTQS010000038        18715 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.038, whole genome shotgun sequence.
ACCESSION   DAWTQS010000038 DAWTQS010000000
VERSION     DAWTQS010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18715
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.038"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            462..1304
                     /locus_tag="WNA25_001316"
     CDS             462..1304
                     /locus_tag="WNA25_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFA9824061.1"
                     /translation="MCQLLGMNCNTPTDIMFSFEGFRRRGGITDHHADGFGIGFFEGK
                     GVRLFHDDKPSANSPVADLVRAYQIKSENVVAHIRKASQGQTSLANTHPFMREMWGGY
                     WLFAHNGHLVDFFPEQGEFFHPVGTTDSERAFCHILNRLRTRFAARPDDDTLFDAIAG
                     LTHEIRKFGLFNFMLSDGISLFAHASTLLHYIVRQAPFGKARLLDDDVMVDFAEVTTP
                     DDRVAVISTLPLTRDESWSQLAVNELVMFREGNIVRHDRPENPVYMSAEEGLEIARAA
                     GVSV"
     gene            1333..1797
                     /gene="nrdR"
                     /locus_tag="WNA25_001317"
     CDS             1333..1797
                     /gene="nrdR"
                     /locus_tag="WNA25_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HFA9824062.1"
                     /translation="MKCPFCSHPDTRVADSRLMEERNTVRRRRHCPGCGKRFGTLETV
                     ELKMPAVIGPDKKRSPFNAQRLRNDLTAAARKSALTPEQIDETVRLTEHRLYTSGQRD
                     ISSSELVEIVLEELFGQNTEAAVRFAALHKRFDSPEHFASWLAQAVKTGGKT"
     gene            1829..2938
                     /gene="ribD"
                     /locus_tag="WNA25_001318"
     CDS             1829..2938
                     /gene="ribD"
                     /locus_tag="WNA25_001318"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFA9824063.1"
                     /translation="MFSDTNISMMENALRLAALGRFSTSPNPRVGCVIAHGRQIVGQG
                     FHVKAGEPHAEVHALRQAGEMAKGATAFVTLEPCSHYGRTPPCAEALLRSGVTRVVAA
                     MRDPNPPVAGKGLVLLKAAGIKTECGLLENKARELNRGFLSRIERRRPFVRLKCAVSL
                     DGKTALSDGSSFWITGEEARADVQVLRAESCAVLTGIGTVLADNPRLNVRAFPTLRQP
                     ARIVLDSRLRLPPNSHLVTDGQSPTYIATLERDEDKLRPYREHAHIRILMPSETADGK
                     IDLHHLMRLLADEGFGEIMVEAGSELTSAFLAENLADEIVLYRSPKILGGGKDLFSLP
                     ENRAALSAPPLWTPVSTEILGHDIKTVFRKNGNAF"
     gene            3133..4551
                     /locus_tag="WNA25_001319"
     CDS             3133..4551
                     /locus_tag="WNA25_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFA9824064.1"
                     /translation="MDTKEILGYAAGSIGCAILAVIILPLLSWYFPADDIGRIVLMQT
                     AAGLAMSVLCLGLDQAYIREYNAAADKDILFKALFLPPLLSAAAIAAFLLSHPALPSE
                     ILFSLDDAAAGIGLVLFGLSLLPIRFLLLVLRMEGRALAFSSAQLVPKSALLLLLLTV
                     WLLHFPARTAVLTSVYALANLAAAAFLLFQNRCRLKAVRRAPFSPAVLHRGLRYGIPL
                     ALDSLAYWGLTSADRLFLKKYAGLEQLGIYSMGISFGGAALLFQSIFSTVWTPYIFRA
                     IEKNAPPTRLSATAESVAALLAAALCLTGIFSPLTSLLLPENYAAVRFTVVSCMLPPL
                     FCTLAEISGIGLNVVRKTRPIAFATLGALAANLLLLGLSVPAGGARGAAVACAASFWL
                     FFVFKTESSCRLWQPLKRLPLYMHTLFCLASSAAYTCFGTPANYPLFAGVWAAYLAGC
                     ILRHRKNLHKLFHYLKKQGFPL"
     gene            4548..5615
                     /locus_tag="WNA25_001320"
     CDS             4548..5615
                     /locus_tag="WNA25_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFA9824065.1"
                     /translation="MKIILTTSMSGLGGTETATVRLGRLLKRHGHDIILASSDGPFVG
                     EAQASGIRWQPVDFYRGGLAGYLKSTFAYARMLRREQPDIIDCQMARVVPACALAAKI
                     VSPKTKIICHSHGLDAATYPKTAKLFDKLGAYIIGNCKHEREKLIRHGFPAGRIAYAY
                     NTPPPEFHFRKTEKECAVLGTLSRLDTVRAVHLMLDILKKMVGRNIPVRLNMAGIGEE
                     MDNLKAQAKRLGIEDKVTFLGGVRDLTGYFKEVDILVNTPHCVGDHGAGVGNNILEAG
                     LYDTPVVTYNMAGISEMVITGQTGYCIPFGDDEAFIEAVDTLIKHPELRSQMGKALHK
                     HVETLCSDDEIYRTTMAAYEM"
     gene            5629..6801
                     /locus_tag="WNA25_001321"
     CDS             5629..6801
                     /locus_tag="WNA25_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFA9824066.1"
                     /translation="MNITIAAPYCSLPSEPHFNRFWYLAELLSQSHDVLLITSNFKHY
                     DKSFRRPEDAEAASQGRLKVILLEESGYSKNVSLGRVTSHHRFVKHFEKWLENCRPGE
                     QDVIYSAYPLIATNLLLGKHKARLGYKLIVDVQDVWPESFSSVVPFLKKVPHKLLPFA
                     SRANRAYRYADALVAVSQTYLDRAKETNPNVPGEVIYIGADFPKLDAAPAKDFGDDKT
                     RFFYLGTLSYSYDVETVCKGVRKLLDDGENVELHIMGGGPDLDRLKQYACDGIKFYGY
                     IPYAEMMSVAKGCDIAVNAIHSYAMQSITNKLSDYMALQKPILNSQVHDEVAEVLTLL
                     PHENYRSGDVDGFVQAAKDILKRKNDPVQSEEIVRRFKRDVAYQKIVNLIERLADE"
     gene            6794..8035
                     /locus_tag="WNA25_001322"
     CDS             6794..8035
                     /locus_tag="WNA25_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NeuD/PglB/VioB family sugar acetyltransferase"
                     /protein_id="HFA9824067.1"
                     /translation="MNKFFKRLIDIIASASGLIVLSPVFLVLIYLIRKNLGSPVFFIR
                     ERPGKDGKPFKMVKFRSMRDALDSDGIPLPDSERLTPFGKKLRTASLDELPELWNVLK
                     GEMSLVGLRPLLMQYLPLYNKFQNRRHEMKPGITGWAQVNGRNALSWDEKFSCDVWYT
                     DNFSFWLDMKILFLTVKKVLIKEGISAQGEATMPPFAGNRKLAVIGAGGHGKVVAELA
                     AALGTYGEIVFLDDRTQGSVNGFPVIGTTLLLENSLSPEQFDITVAVGNNRIRRQITE
                     KAAALGFKLPVLIHPDATVSPSAIIGQGSVVMAKAVVQAGSVLKDGVIVNTAATVDHD
                     CLLDAFVHISPGAHLSGNTRIGEESRIGTGACSRQQTTVGSGVTAGAGAVIVCDIPDG
                     MTVAGNPAKPLTGKNPKTGTA"
     gene            8166..9341
                     /locus_tag="WNA25_001323"
     CDS             8166..9341
                     /locus_tag="WNA25_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HFA9824068.1"
                     /translation="MLNTALSPWPSFTREEADAVSKVLLSNKVNYWTGSECREFEKEF
                     AAFAGTRYAVALSNGTLALDAALKAIGIGAGDDVIVTSRTFLASASCIVNAGANPVFA
                     DVDLNSQNISAETVKAVLTPNTKAVIVVHLAGMPAEMDGIMALAKEHDLWVIEDCAQA
                     HGATYKGKSVGSIGHVGAWSFCQDKIITTGGEGGMVTTNDKTLWEKMWAYKDHGKSYD
                     AVYHREHAPGFRWLHESFGTNWRMMEMQAVIGRIQLKHLPEWTARRQENAAKLAESLR
                     KFKSIRLIEVAGYIGHAQYKFYAFVKPEHLKDDWTRDRIVSELNARNVPCYQGGCSEV
                     YLEKAFDNTPWRPKERLKNAVELGGTALTFLVHPTLTDDEIAFCKKHIEAVLTEAAR"
     gene            9389..11298
                     /locus_tag="WNA25_001324"
                     /pseudo
     CDS             9389..11298
                     /locus_tag="WNA25_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222987.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
     gene            11354..12646
                     /locus_tag="WNA25_001325"
     CDS             11354..12646
                     /locus_tag="WNA25_001325"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFA9824069.1"
                     /translation="MQFSAFGEKFTQHSGILQLMDDLGDALKSDKPVNMLGGGNPARI
                     PEIDRAFADIFSKLAAEHAVENIGNYSNPQGDAALIDALTAFPNREYGWNLTVGNIAL
                     TNGSQNAFFYLFNLFGGKFKLSDGTSAEKAILLPLAPEYIGYADVHVEGRHFVSVKPK
                     IENVEHEGEAGFFKYRVDFDALENLPELKAGKIGAICCSRPTNPTGNVLTDGEMARLD
                     ALAREHGIPPIIDNAYGMPFPNIIDSGVTLNWHENIILCFSLSKVGLPGVRTGIIVAA
                     PEAVKAVSSLNAIVNLAPTRFGAAIAAPLLESGEMKRLADQVIRPFYRNQAQTAVSLL
                     KRELGAYPMKIHKPEGAIFLWLWFENLPVSSQTLYEMLKAEGTLIIPGEHFFVGIDTQ
                     DYPHAGECIRMSIAQDAQTLEKGIAAIGKTVRKPYDNV"
     gene            complement(12698..13534)
                     /locus_tag="WNA25_001326"
     CDS             complement(12698..13534)
                     /locus_tag="WNA25_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFA9824070.1"
                     /translation="MIDLHCHSTVSDGMLPPAEVVRLAHQNGCTLLALTDHDHTGGIS
                     EARAEADRLGLRLINGVEISVTWRGRTIHVVGLDFDEQDENLQNLLADVRKGRLKRLE
                     AIAAKLEKKGIGGAYDGALALAANKEMVSRTHVAEFLIKNGHVKNKRQAFTKYLGDGK
                     SCAVRHEWATLADCVSAVNGAGGMAVIAHPMRYDLSATAKRNLFEEFKNLGGAGIEVH
                     SGNCCKNDRLNYALLADRFGLLASAGSDFHRLDDFSGGILGACPDLPENCKPVRAHFS
                     RH"
     gene            complement(13829..14032)
                     /locus_tag="WNA25_001327"
     CDS             complement(13829..14032)
                     /locus_tag="WNA25_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824071.1"
                     /translation="MDTTLKHKAEALLGEPLLDEPVRPESWECCGSDCGEACIQTIYW
                     ADKARYDAQRKKLKEAGWPDDAV"
     gene            complement(14010..14260)
                     /locus_tag="WNA25_001328"
                     /pseudo
     CDS             complement(14010..14260)
                     /locus_tag="WNA25_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582378.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
     gene            complement(14272..14469)
                     /locus_tag="WNA25_001329"
     CDS             complement(14272..14469)
                     /locus_tag="WNA25_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824072.1"
                     /translation="MCISVNADSSGNRICSLPLGRISLDGWRHTGFQTAFLRNRQCGH
                     IAGFAAILGAAAAGATITPSF"
     gene            14793..18227
                     /gene="dnaE"
                     /locus_tag="WNA25_001330"
     CDS             14793..18227
                     /gene="dnaE"
                     /locus_tag="WNA25_001330"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFA9824073.1"
                     /translation="MTEPTYIPLRLHTEFSITDGMVRIKKLIAKAQEYGLPALGISDL
                     MNEFGLVKFYKACRGAGIKPVGAADVWIGNPNAPDKPFRAMLVIRNDAGYLRLSELLT
                     EAYVGQDRNIHHAELNPEWLENGDNSGLICLSGAHYGEVGVNLLNGNEDAARAAALKY
                     AAWFPDTFYLELQRLPERPEWEACVSGSVKLAEELGLPVVATHPTQFMNRDDFNAHEA
                     RVCIAGGWVLTDKKRPRDFTPSQFFIPPETMLERFADLPEALENTVEIAKRCNLHITL
                     GKNFLPLFPTPDGLSLDDCLIKLSNEGLQERMVQLYPDEAERAAKMPEYQERLDFELN
                     IIIQMKFPGYFLIVQDFINWAKTHGCPVGPGRGSGAGSLVAYSLKITDLDPLKYALLF
                     ERFLNPERVSMPDFDVDFCQANRGRVIEYVREKYGAQAVSQIVTFGTMSSKAVIRDVG
                     RVLELPFMLCDKLSKLIPLEANKPLSLDDAMKAQPQIQELLEAEEADELITLAKKLED
                     LTRGLGMHAGGVLIAPGKISDYSPVYQADESASPVSMYDKGDVEDVGLVKFDFLGLRN
                     LTIIEMAQNNIKNTAGDIVDVGKIPLDDQTAYQIFRDANTTAVFQFESTGMKKMLKTA
                     HTTKFEELIAFVSLYRPGPMDNIPDFVARMKGQEFQYIHPLLEGILAPTYGIMVYQEQ
                     VMQAAQIIGGYSLGGADLLRRAMGKKKPEEMVKHREIFAEGAAKQGISREKSDEIFNY
                     MEKFAGYGFNKSHAAAYALISYQTAWLKAHYPAEFMAATMSSELDNTDQLKHFYDDCR
                     ANGIEFLPPDINESDYRFTPYPNMKIRYALGAIKGTGEAAVESIIAARQSGGKFTGLL
                     DFCERVGKEHMNRRTLEALIRGGAFDSIEPNRAMLLANIDLAMDNADQKAANANQGGL
                     FDMMEDAIEPVRLIDAPMWSESEKLAEEKTVIGFYLSGHPFGPYAQEVRQIAPTKLGR
                     LKPQDSVRLAGFVTAVRTMMGKRGKIAFVSLEDSSGQAEIMVGGQTLENCADCLKADQ
                     VLIIESKVSRDDYGGGDGLRIMANQVMTLQTARERYARSLSLALAPHHDIGGLVRLLA
                     AHQLPDTPRIPLQLSYANEKASGRLQVPPKWTVTPSSALFGELETLLGSRSVRVNW"
BASE COUNT         4283 a         5802 c         4810 g         3820 t
ORIGIN      
        1 tagggaaccg ttccctttga gccggggcgg ggcaacgccg taccggtttt tgttaatccg
       61 ctataacagc aaccctgtcg ccgtcattcc cgcgaaagcg ggaatccagt ccgttcagtt
      121 tcggtcattt ccgataaatt cctgttgctt ttcatttcta gattcccact ttcgtgggaa
      181 tgacggcgga agggttttgg ttttttccga taaattcttg aggcattgaa attctagatt
      241 cccgcctgcg cgggaatgac ggctcaaaag ttacggaacg aaaaacaacc gaaaccggac
      301 aagtcggatt cccgcctgcg cgggaatgac ggaatcttaa gtttccgtct ttgttttctg
      361 ttttcgcggg aataatgggg aacaagccgt atttcagacg gcattttcag ttcggggtat
      421 aatccgaata cttgcgacca tctgaatcat ttggacaaac tatgtgtcaa ctgctgggca
      481 tgaactgcaa tacgccgacc gatattatgt tttcctttga aggcttccgc cgcaggggcg
      541 gcattaccga ccatcacgcc gacggtttcg gcatcggctt tttcgaaggc aaaggcgtgc
      601 gcctgttcca tgacgacaag ccgagtgcaa attcccccgt cgccgacctc gtgcgcgcct
      661 accagattaa atcggaaaac gtcgtcgccc acatccgcaa agcctcgcaa ggtcaaacct
      721 cgctggcgaa cacccatccc tttatgcgcg aaatgtgggg cggctactgg ctgtttgccc
      781 acaacgggca tttggtcgat tttttccccg aacaaggaga atttttccac cccgtcggca
      841 caaccgattc cgaacgcgcg ttctgccaca tcctcaaccg cctgcgcacc cgctttgccg
      901 cccgtcccga cgacgacacg ctgtttgacg cgattgcggg gctgacgcac gaaatccgca
      961 agttcgggct gtttaacttc atgctttcag acggcatttc cctgtttgcc cacgccagca
     1021 cgctgctgca ctacatcgtc cgccaagccc cgttcggcaa agcgcgcctg ctcgacgacg
     1081 atgtaatggt cgattttgcc gaagtaacca cgccggacga ccgcgtcgcc gttatctcca
     1141 ccctgccgct gactcgcgat gaatcgtggt cccaacttgc cgtgaacgaa ctggtcatgt
     1201 tccgcgaagg caacatcgtc cggcacgacc gtcccgaaaa tcccgtctat atgagtgccg
     1261 aagaaggcct ggaaatcgcc cgcgccgccg gcgtatcggt ttgacgttca gacggcatca
     1321 ggaagaaaca ccatgaaatg cccgttttgc agccacccag acacccgagt tgccgattcc
     1381 cgcctgatgg aagagcgcaa taccgtccgc cgccgccgcc actgccccgg ctgcggcaaa
     1441 cgcttcggca cgctcgaaac cgtcgaactc aaaatgcccg ccgtcatcgg tccggacaag
     1501 aaacgttcgc ccttcaatgc acaacgcctc cgcaacgacc tgaccgccgc cgcccgaaaa
     1561 tccgccctga cacccgaaca gattgacgaa accgtccgcc tgacggaaca caggctctac
     1621 acttcgggtc agcgcgacat atcctcatcc gaacttgtcg aaatcgtgct ggaggagctg
     1681 ttcgggcaaa acacggaagc cgccgtccgt ttcgccgccc tgcacaaacg cttcgacagc
     1741 ccggaacatt tcgcctcgtg gctggcgcaa gccgtcaaaa caggcggcaa aacctgattc
     1801 ccccaaccca tactgatacg gtatccctat gttttcggac acaaatatat ccatgatgga
     1861 aaacgccctc cgacttgccg ctttggggcg tttttccact tcacccaacc cgcgcgtcgg
     1921 ctgcgttatc gcacacggca gacaaattgt cggacagggc ttccacgtca aagcgggcga
     1981 accccatgcc gaagtccacg ccctgcgtca ggcgggggaa atggcaaaag gcgcgaccgc
     2041 ctttgttacc ctcgaaccgt gcagccatta cggacgcaca ccgccctgcg ccgaagccct
     2101 gctccgttcc ggcgtaacac gcgtcgtcgc cgccatgcgc gaccccaacc cgccggttgc
     2161 aggcaaaggg cttgtcctgc tcaaagcagc gggcatcaag acggaatgcg gactacttga
     2221 aaacaaagca cgcgaactca accggggatt tttatcgcgc atcgaacgcc gccgcccctt
     2281 tgtccgcctc aaatgcgccg tttcgctgga cggcaaaacc gccctttcag acggcagcag
     2341 cttttggatt accggcgagg aagcgcgtgc cgacgtacag gttttgcgtg ccgaaagctg
     2401 cgcggtgctg accggcatcg gcacggtgtt ggcagacaat ccccggctca acgtccgcgc
     2461 ttttccaact ttgcgccaac ccgcacgcat cgttttagac agccgcctgc gcctgccccc
     2521 gaacagccat ttggttaccg acggacaatc tccgacctac atcgccacct tggaacgcga
     2581 tgaagacaaa ctccgcccct atcgggaaca cgcacacatc cgcatcctga tgccgtctga
     2641 aacggcagac ggcaaaatcg acctgcacca tctgatgcgc ctccttgccg acgaaggttt
     2701 cggcgaaatc atggtcgaag caggctccga actcacatcc gcatttttgg cggaaaattt
     2761 ggcggacgaa atcgtgctgt accgttcgcc caaaatcctc ggcggcggca aagacctgtt
     2821 ttccctgccc gaaaaccgcg ccgccctttc cgcgccgcca ttatggacac ccgtttccac
     2881 cgaaatcctc ggacacgaca tcaaaaccgt gttccggaaa aacggcaacg ccttttaaag
     2941 ggtttgccgc cgtttcacta tataataacg ccgataaaaa acggcccgtt cagaccgccg
     3001 cccgcccgga aaagcgcaac ccgggctgcc gcaccccgcc ggcagccgcg acggcctgaa
     3061 agccgtcaaa ttccgatcaa gaaaggcttc agacggcaca ggcagcatcc cgccgccgac
     3121 cggacatcaa aaatggacac aaaagaaatc ctcggctacg cggcaggatc gattggctgc
     3181 gcgattttag ccgtcatcat cctgccgctg ctgtcgtggt atttccccgc cgacgacatc
     3241 gggcgcatcg tgttgatgca gacggcggcg ggactggcga tgtcggtgtt gtgcctcggg
     3301 ctggatcagg catatatccg cgaatacaat gccgccgccg acaaagacat tttgttcaaa
     3361 gccctgttcc tgccgccgct gctgtctgcc gccgcgatag ccgccttcct gctttcccac
     3421 ccggccctgc cgtctgaaat cctgttttcg ctcgacgatg ccgccgccgg catcgggctg
     3481 gtgctgtttg gactgagctt gctgcccatc cgctttctct tactggtttt gcgtatggaa
     3541 gggcgcgccc ttgccttttc gtccgcgcaa ctcgtaccca agtccgccct gctgctgttg
     3601 ctgctgacgg tctggctgct gcactttccg gcgcgcaccg ccgtcctgac ctccgtttac
     3661 gcgctggcaa accttgccgc cgccgccttt ttgctgtttc aaaaccgatg ccgtctgaag
     3721 gccgtccggc gcgcaccgtt ttcgcccgcc gtcctgcatc gcgggctgcg ctacggcata
     3781 ccgctcgcac tggacagcct tgcctattgg ggactgacat ccgccgaccg tttgttcctg
     3841 aaaaaatatg ccggcctgga acagctcggc atttattcga tgggtatttc gttcggcggg
     3901 gcggcattat tgttccaaag catcttttca acggtttgga caccgtatat tttccgcgca
     3961 atcgaaaaaa acgccccgcc cacccgcctc tcggcaacgg cagaatctgt cgccgccctg
     4021 cttgccgccg ccctctgcct gaccggcatt ttctcgcccc tcacctccct cctactgccg
     4081 gaaaactacg ccgccgtccg gtttaccgtc gtatcgtgta tgctgccgcc gctgttttgc
     4141 acgctggcag aaatcagcgg catcggtttg aacgtcgtcc gcaaaacgcg tccgattgca
     4201 ttcgccacct tgggcgcgct ggcggcaaac ctgctgctgc tggggctttc cgtaccggcc
     4261 ggcggggcgc ggggcgcggc ggttgcctgt gccgcttcat tctggctgtt ttttgttttc
     4321 aagaccgaaa gctcctgccg cctgtggcag ccgctcaaac gcctgccgct ttatatgcac
     4381 acattgttct gcctggcctc ctcggcggct tacacctgct tcggcacgcc ggcaaactac
     4441 cccctgtttg ccggcgtatg ggcggcatat ctggcaggct gcatcctgcg ccaccggaaa
     4501 aatttgcaca aactgtttca ttatttgaaa aaacaaggtt tcccattatg aaaatcatcc
     4561 tgactacttc tatgtcgggt ctgggcggca cagaaaccgc caccgtccgc ctcgggcggc
     4621 tgctcaaacg gcacgggcac gacatcatcc tcgcctcctc ggacggccct tttgtcggag
     4681 aggcgcaagc atcgggcatc cgttggcagc cggttgattt ttaccggggc gggcttgccg
     4741 gctaccttaa aagcaccttt gcctacgccc ggatgctgcg ccgcgaacaa cccgacatca
     4801 tcgactgcca aatggcgcgc gtcgtcccgg cctgcgccct tgccgccaaa atcgtttcac
     4861 cgaaaaccaa aatcatctgc cactcgcacg gtttggacgc ggcaacctac cccaaaaccg
     4921 ccaaactctt cgacaagctg ggcgcgtaca tcatcggcaa ctgcaaacac gaacgcgaaa
     4981 agctcatccg ccacggcttc cccgccggcc ggattgccta cgcctacaac accccccccc
     5041 cggaatttca tttccggaaa accgaaaaag aatgcgccgt actcggcaca ctttcccgtt
     5101 tggacaccgt ccgcgccgtg catctgatgt tggatatttt gaagaaaatg gttggccgca
     5161 atatacccgt acgcctgaac atggcgggca taggagaaga aatggacaac ctcaaagccc
     5221 aagccaaacg tttgggtatt gaagacaaag tgaccttcct cggcggcgtc cgcgatttga
     5281 ccggctactt caaagaagtc gatattttag tgaacacgcc gcattgcgta ggtgaccacg
     5341 gcgcaggtgt cggcaacaat attttggaag ccggccttta cgacacgcct gtcgtgacct
     5401 acaacatggc gggcatttcc gaaatggtca ttaccggcca aaccggctac tgcattcctt
     5461 tcggcgatga cgaagcgttt atcgaagccg tcgatacact catcaagcat ccagagttgc
     5521 gcagccaaat gggcaaagcc ctgcacaaac atgtcgaaac cttatgctcc gacgacgaaa
     5581 tctaccgcac caccatggct gcgtacgaaa tgtaaggccg tctgaaacat gaacattacc
     5641 atagccgccc cctactgctc gctgccgtcc gaaccgcatt tcaaccgctt ttggtatctg
     5701 gcagaactat tgtcgcaatc gcacgacgtg ttgctgatta ccagcaactt caagcactac
     5761 gacaaatctt tcagacggcc cgaagatgcc gaggccgcct cacaaggccg tctgaaagtc
     5821 atactgttgg aagaaagcgg atacagcaaa aacgtatctt tgggacgcgt gaccagccat
     5881 caccgcttcg tcaaacattt tgaaaaatgg ttggaaaact gccgtccggg cgaacaagac
     5941 gtcatctatt ccgcctatcc gctgattgcc accaacctgc tgttgggcaa acacaaagcg
     6001 cgtttgggtt acaaactgat tgtcgatgtg caggacgtat ggccggagtc tttctcctcg
     6061 gtcgtaccgt ttttgaaaaa agtaccgcac aagctgctgc cctttgcttc acgcgccaac
     6121 cgggcctacc gctacgccga cgcgctggtt gccgtatcgc agacttatct cgaccgcgcc
     6181 aaagaaacca atccgaacgt acccggcgaa gtcatctata ttggtgctga ctttcccaaa
     6241 ctcgatgccg cacctgccaa agattttggc gacgacaaaa cccgcttttt ctacttgggc
     6301 acgctcagtt acagctatga cgtggaaacc gtgtgcaaag gcgttcggaa acttttggac
     6361 gacggcgaaa atgtagagct gcacatcatg ggcggcggcc ccgatttgga caggctcaaa
     6421 caatatgcct gcgacggcat caagttttac ggctacattc cttacgccga aatgatgtcg
     6481 gtcgccaagg gctgcgacat tgccgtcaac gccatccact cttacgccat gcagtcgatt
     6541 accaacaaac tctccgacta tatggctttg caaaaaccga ttctgaacag ccaggtccac
     6601 gacgaagttg ccgaagtcct taccctgctg ccgcacgaaa actaccgttc cggcgatgtg
     6661 gacggtttcg tccaagccgc caaagatatt ttgaagcgca aaaacgatcc tgttcagtcc
     6721 gaagaaatcg tccgccgttt caagcgcgac gttgcctatc aaaaaatcgt caacctgatt
     6781 gaaagattag ctgatgaata aatttttcaa acgcctcatc gacatcatcg catccgcatc
     6841 ggggctgatt gtcctgtcgc ccgtgtttct ggttttgata tacctcatcc gcaagaatct
     6901 gggttcgccc gtcttcttca ttcgggaacg ccccggaaag gacggaaaac cttttaaaat
     6961 ggtcaaattc cgttccatgc gcgacgcgct tgattcagac ggcattccgc tgcccgacag
     7021 cgagcggctc acgcccttcg gcaaaaaact gcgcaccgcc agtttggacg aactgcctga
     7081 actgtggaac gtcctcaaag gcgagatgag cctggtcggc ctccgcccgc ttttgatgca
     7141 gtatctgccg ctttacaaca aatttcaaaa ccgccgccac gaaatgaaac cgggcattac
     7201 cggctgggcg caggtcaacg ggcgcaacgc gctttcgtgg gacgaaaagt tctcctgcga
     7261 tgtttggtac accgacaatt tcagcttttg gctggatatg aaaatcctgt ttctgacagt
     7321 caaaaaagtc ttgattaaag aaggcatttc ggcgcaaggg gaagccacca tgcccccttt
     7381 cgcggggaat cgcaaactcg ccgttatcgg cgcgggcgga cacggcaaag tcgttgccga
     7441 gcttgccgcc gcactcggca catacggcga aatcgttttt ctggacgacc gcacccaagg
     7501 cagcgtcaac ggcttccccg tcatcggcac gacgctgctg cttgaaaaca gtttatcgcc
     7561 cgaacaattc gacatcaccg tcgccgtcgg caacaaccgc atccgccgcc aaatcaccga
     7621 aaaagccgcc gcgctcggct tcaaactgcc cgttctgatt catcccgacg cgaccgtctc
     7681 gccttctgca ataatcggac aaggcagcgt cgtaatggcg aaagccgtcg tacaggccgg
     7741 cagcgtattg aaagacggcg tgattgtgaa cactgccgcc accgtcgatc acgactgcct
     7801 gcttgacgct ttcgtccaca tcagcccggg cgcgcacctg tcaggcaaca cgcgtatcgg
     7861 cgaagaaagc cggataggca cgggcgcgtg cagccgccag cagacaaccg tcggcagcgg
     7921 ggttaccgcc ggtgcagggg cggttatcgt atgcgacatc ccggacggca tgaccgtcgc
     7981 gggcaacccg gcaaagcccc ttacgggcaa aaaccccaag accgggacgg cataagcgat
     8041 taaaataaac ttccgtacaa accgattttg acaacacctg cggcgcgcgc ccgattcttc
     8101 ggaacacgcc cccttcagac ggcatagggt cggaaatgcc gtccgaaccc gacggaccaa
     8161 acaccatgct gaacaccgcc ctttccccat ggccgagctt cacccgagaa gaagccgatg
     8221 ccgtttccaa agtcctgctg tccaacaaag tcaactactg gacgggcagc gaatgccgcg
     8281 aatttgaaaa agaatttgcc gcctttgccg gcacgcggta cgccgtcgcc ctttccaacg
     8341 gcacgcttgc gctcgatgcc gcactcaaag caatcggcat aggcgcgggc gacgatgtga
     8401 ttgttacctc gcgcaccttc ctcgcctccg cctcctgcat tgtgaacgcg ggcgcaaacc
     8461 ccgtgtttgc cgacgtggat ttgaacagcc aaaacatcag cgcggaaacc gtcaaagccg
     8521 tgctgacccc gaataccaaa gccgtcattg tcgtccacct tgccggtatg cctgccgaaa
     8581 tggacggcat catggctttg gcaaaagaac atgatttgtg ggtgattgaa gactgcgccc
     8641 aagcgcacgg cgcgacctac aaaggcaaat ccgtcggctc tatcgggcac gtcggcgcgt
     8701 ggtcgttctg tcaagacaaa atcatcacca ccggcggcga gggcggtatg gttacgacca
     8761 acgacaaaac cctgtgggaa aaaatgtggg cgtacaaaga ccacggcaaa agctacgatg
     8821 ccgtgtacca ccgcgaacac gcgcccggtt tccgctggct gcacgaaagt ttcggcacca
     8881 actggcgcat gatggaaatg caggctgtca tcggccgcat ccagctcaaa cacctgcccg
     8941 aatggacggc gcgccgccaa gaaaacgccg ccaagctggc agaaagtttg cgcaaattca
     9001 aaagcatccg cctgattgaa gtcgccggct acatcggaca cgcgcaatac aagttctacg
     9061 ccttcgtcaa acccgaacac ctgaaagacg actggacgcg cgaccgcatc gtcagcgaac
     9121 tgaacgcgcg caacgtcccc tgctatcaag gcggctgctc cgaagtgtat ttggaaaaag
     9181 ccttcgacaa cacgccgtgg cggcccaaag agcggctgaa aaacgccgtc gaactgggcg
     9241 gcaccgccct gacgttcttg gtgcacccga cgctgaccga cgacgaaatc gcgttctgca
     9301 aaaaacacat cgaagccgtc ctgaccgaag ccgcacgata acccttcaga cggcatatgc
     9361 cgcctgaaag cacataccgc cccacgatat gaatctggaa accctgatcg ccctgccgcg
     9421 caacatcaag aaaatctgtt tcctcataca cgattttctg atgattttca ttgccttttg
     9481 gttcacccaa agcctgaagg ccgactactc ggacgaatgg ttcgattttg ccaactggca
     9541 gtcttttttg ctgactgcct tgctgaccat cacattattt atccgaatgg ggctttaccg
     9601 cgccgttaca cgctttgtca gtttccacgt cctgaccacc gcctttgccg gcagcctcgc
     9661 ctccgccgta ttgttcttcc tcaacacatt gatttttgaa gaaaggctgc gcctcgccct
     9721 gccgattgtc tatttcttac tgctgtttgt ttccgtgacc ggctcgcgta tggttttgcg
     9781 cggactcctg tccgaccacc agaaaaaaca catgaccccg tcatcattta cggcgcgggg
     9841 cgttcgggtc ggcagctcct tgaagccgtc aaacagatgc gcgaatattc cgccgccgcc
     9901 tttgtggacg acaaccccaa gctttggcac accgtcatct acgaccttgc cgtttaccag
     9961 cccgatgcca tcgccttcct catcgaacgc tacggcgtgg aaaaaatcct gctcgccatt
    10021 cccagcgcga ctcaggaaca acgccgccga atcatcagca aactgaaagc ctatccgtgc
    10081 gaagtgttga ccattcccgg aatgaaagac ctgatggacg ggaaaatcag catcggcacg
    10141 ctcaaaaaaa tctctgtgtc cgacctgctc gggcgcgatt ccgtcgcacc cgacgaccgc
    10201 ctcatgaatg ccgacaccga aggcaaaacc gtcatggtaa ccggcgcggg cggctccatc
    10261 ggttcggaac tctgccgcca gattatccgc cgccgccccg aaaggctgct gctgttcgag
    10321 ctgtccgaat tcgccctgta caccgttgaa aaagaattgt gcgaatactg cgcccgaaac
    10381 ggcatcgccg ccgaaatcct tccctttttg ggttcggtgc aaaaccgcac gctgctcacg
    10441 cacattatga ccgccttttc cgttgcgacc gtctatcacg ccgccgccta caaacacgtc
    10501 cctatggtcg agttcaacac cgtcgagggc atacgcaaca acatcttcgg cacactcgaa
    10561 tgcgcgcttg ccgccacggc ttcgggcgtg gaaaccttcg tcctcatctc caccgacaaa
    10621 gccgtccgcc ccgccaacac gatgggtgcg ggcaaacgca tggcggaact ctgccttcag
    10681 gcactcgccg ccgaacccgg acaaaaaacc cgcttcagca tggtacgttt cggcaatgtt
    10741 ttaggctcgt ccggctccgt cgtcccgctg tttgaaaaac agattgccga aggcggcccc
    10801 atcaccctga cccaccccga catcacgcgc tatttcatga ccatacccga agcggcgcaa
    10861 ctggttatcc aagccggcgc gatgggcaga ggcggcgacg tgttcgtcct cgatatgggc
    10921 gaatccgtca gaatcaccga ccttgcccgc caaatgatta ccttaagcgg actcaaaccc
    10981 aaaacgcccg aacagcccga cggcgacatc gaaatcctca ttaccgggct gcgccccggc
    11041 gaaaaactct acgaagagtt gctcatcggc gacaacgtcc gcaaaaccgg ccatccgcgc
    11101 atcatgaccg ccgacgagac catgctgccg tggcacgaac tctccgccct gctcgaccgc
    11161 atccgcaccg cctgcgaccg ttacgaccag caggcaatcc gcgacctgct cgtcaacgcc
    11221 ccgaccggct ttaccccgac cggcggcatc tgcgacctgc tttgggtatg cgaaacacac
    11281 agaaaaaatg ccgtctgaac cttcagacgg cataccgtac aaaccaatct gccttacaca
    11341 cactgagttt gacatgcagt tctcggcatt cggcgaaaaa ttcacgcaac acagcggcat
    11401 cctgcaactg atggacgacc tcggcgacgc gctcaaaagc gacaagcccg tcaatatgct
    11461 cggcggcggc aacccggcgc gcattcccga aatcgatcgg gcgttcgccg acatattttc
    11521 caagctggcg gcggaacacg ccgtcgagaa catcggcaac tactccaatc cccaaggcga
    11581 tgccgcgctg attgacgcgc tgaccgcctt ccccaaccgc gaatacggct ggaacctgac
    11641 cgtcggcaat atcgcgctga ccaacggttc gcaaaacgcg tttttctatt tattcaacct
    11701 cttcggcggc aaattcaagc tttcagacgg cacatccgca gaaaaagcca ttttgttgcc
    11761 gctcgcgccc gaatacatcg gctatgccga cgtgcatgtc gaagggcggc acttcgtttc
    11821 cgtcaaaccc aaaatcgaaa acgtcgaaca cgaaggcgaa gcaggcttct tcaaataccg
    11881 cgtggacttt gacgcactgg aaaacctgcc cgaactcaaa gcgggcaaaa tcggcgcgat
    11941 ttgctgttcg cgcccgacca acccgaccgg caacgtgttg accgacggcg aaatggcgcg
    12001 tttggacgct ttggcgcgtg aacacggcat tccgccgatt atcgacaacg cctacggaat
    12061 gccgttcccc aacattattg acagcggcgt aacgctgaat tggcacgaaa acatcatcct
    12121 ctgcttcagc ctttccaaag tcggcctgcc gggcgttcgc accggcatca tcgtcgccgc
    12181 gcccgaagcc gtcaaagccg tcagcagcct gaacgcgatt gtgaaccttg cccccacgcg
    12241 cttcggcgcg gccatcgccg ccccgctgct ggaaagcggc gagatgaaac ggcttgccga
    12301 ccaagtcatc cggccgtttt accgcaatca ggcgcaaacc gccgtttcgc tgctcaagcg
    12361 cgagctgggc gcgtacccga tgaaaatcca caaacccgaa ggcgcgattt tcctgtggct
    12421 ctggtttgaa aacctgcccg tttcgtcgca aaccctgtac gaaatgctca aagccgaagg
    12481 tacgctgatt attccgggcg aacatttctt cgtcggcatc gacacgcaag actacccgca
    12541 cgcgggcgag tgcatccgta tgagtatcgc acaggacgct caaacgctgg aaaaaggcat
    12601 cgccgccatc ggcaaaaccg tccgaaaacc gtacgacaac gtttaaaacg caaaaaatgc
    12661 cgtctgaaga cctttcagac ggcattttca tccgcattca atgtcgggaa aaatgcgccc
    12721 gcaccggttt gcagttttcc ggaagatcgg gacacgcgcc gaggatgccg ccgctgaagt
    12781 cgtctaaacg gtggaagtcg ctgcccgcgc ttgccaacaa tccgaagcgg tctgccaaaa
    12841 gcgcgtagtt gaggcggtcg tttttgcagc agttgccgct gtggacttcg atgcccgcgc
    12901 cgccgaggtt tttaaattct tcaaacaggt tgcgcttggc ggttgccgac aaatcataac
    12961 gcatcggatg ggcaatcacc gccatgccgc ccgcgccgtt gacggcggag acgcagtctg
    13021 ccagcgtcgc ccattcgtgg cgtaccgcgc aggatttgcc gtcgcccaag tatttggtga
    13081 acgcctgccg cttgtttttg acgtgtccgt ttttgatgag gaactcggcg acgtgggtgc
    13141 ggctgaccat ttctttgttt gccgccagcg ccagcgcgcc gtcgtatgcg ccgccgatgc
    13201 ctttcttttc gagcttggcg gcgatggctt caagacgttt cagacggcct ttgcggacat
    13261 ctgccaacag gttttgcagg ttttcgtcct gctcgtcgaa atccaaaccg acaacgtgta
    13321 tggtacgccc gcgccacgtt accgaaattt ccacgccgtt aatcaggcgc aaaccgagcc
    13381 tgtcggcttc ggcacgcgct tcggagatgc cgcccgtgtg gtcgtggtcg gtcaacgcca
    13441 gcagcgtgca gccgttttga tgtgcgaggc ggacgacttc ggcggggggg agcataccgt
    13501 cggaaacggt ggaatggcag tgcaggtcta tcatggggtg ttatgtggtg gtgaatgaag
    13561 gacgggatgt ttcaatgttg tcggacagtg tggcggcggc ggtcatttta aaaaacaggt
    13621 gtatggcaaa tgcaaaggaa aagccgctat gccgtcattc ccgcgcaggc gagaatccag
    13681 actttgattt attggaaatg tttaaggtta accgctattt caaacttccg gattcctgct
    13741 ttcgagggaa tggcgatatg aacgttttca attttaatct actataaaag accgtctgaa
    13801 aacgtggttt tatcaagccc aaccgttttc agacggcatc gtccggccaa cccgcttcct
    13861 tcagtttttt ccgttgcgca tcgtagcgtg ccttgtccgc ccagtaaatc gtctgaatgc
    13921 acgcctcgcc gcagtcgctg ccgcagcatt cccacgactc ggggcggacg ggttcgtcca
    13981 aaagcggctc gcccaaaagg gcttcggctt tatgcttcag ggtcgtatcc atcggcgatt
    14041 tccaagcgcg cgccgtcaaa ctcgatgact tctccgccgc gtattttggc cgttttggta
    14101 ttttggccgt tttgcggatt tcggttccgc cgttgcgcaa caccagccct tcggcgataa
    14161 atgcttttgc ctgtccgccg ctttcggcaa gtccggccaa tttcaagagg tcgcacaaag
    14221 cgatgtattc gttgtcttcg agatagacgg tggcttccat aatgtcccct tttagaaaga
    14281 gggcgttatt gtagcacctg ccgccgccgc acccaaaatt gccgcaaaac cggcgatgtg
    14341 tccgcactgc ctgttccgta aaaatgccgt ctgaaacccc gtatgccgcc atccgtccaa
    14401 agaaatcctg cccaacggca ggctgcaaat cctgttcccc gacgaatccg cattgacgct
    14461 gatgcacatc ctcaaacgcg gactgcccga tacaccggca atcggcatca aaacgaaatc
    14521 aaaaacctgt ttgaacgtaa aatctattta actgcttgcc gtccgtccga ataaggtata
    14581 tagttcctta taactagttt gatagtcctt tatatctatc aatactcctt gggaagcctc
    14641 cgccacacgg caggaggcat ttttttgccg tcgtaaaagc tcaaaaaaca tttgcaggtc
    14701 atgccgtctg aatccgaaac ggcattacct gcaccgccat ctaaagacaa ccctgctaca
    14761 atacgccttt tattgtccac gccgattttg ccatgaccga gccgacctac atccccctgc
    14821 gcctgcatac cgaattctcc attaccgacg gtatggtgcg gattaaaaaa ctgattgcca
    14881 aagcgcaaga atacggtttg cctgctttgg gcatcagcga tttgatgaac gaattcggtt
    14941 tggtgaaatt ctataaagcc tgccgcggcg cagggattaa gcccgtcggc gcggcggacg
    15001 tgtggatagg caatccgaat gcgcccgaca agccgttccg cgccatgctg gttatccgca
    15061 acgatgcggg ttatctgcgc ttgagcgagc ttttgacgga ggcttatgtc ggccaagacc
    15121 gcaatatcca tcatgcggaa ctcaatcccg aatggctgga aaacggcgac aacagcggct
    15181 tgatttgttt gagcggcgcg cattacggcg aagtgggcgt gaatctgttg aacggcaatg
    15241 aagacgcggc gcgtgcggcg gcgttgaaat atgcggcatg gtttcccgat acgttttatt
    15301 tagagctgca acgcctgccc gaacgccccg aatgggaggc ttgcgtttcc ggcagcgtga
    15361 agctggcgga ggaactgggt ttgccggtgg tagcaacgca tccgacacag tttatgaacc
    15421 gcgacgattt caacgcgcac gaggcgcggg tgtgcatcgc gggcggctgg gtgctgacgg
    15481 acaagaaacg tccgcgcgat ttcacgccga gccagttttt cattccgccg gaaacgatgc
    15541 tggagcgttt cgccgacttg cccgaagcct tggaaaacac ggtggaaatc gccaaacgct
    15601 gcaacttgca catcacattg ggcaaaaact tcctacccct tttccccacg cccgacggtt
    15661 tatcactcga tgactgtctc atcaaacttt ccaacgaggg tttgcaggaa cgcatggttc
    15721 agctttatcc cgacgaggcg gaacgggcgg caaaaatgcc ggaatatcag gagcggctgg
    15781 actttgagct gaacatcatc atccaaatga aattccccgg ctatttcctt atcgtacaag
    15841 actttatcaa ctgggcgaaa acgcacggct gtccggtcgg gccgggacgc ggttcgggcg
    15901 cgggttcgct ggtggcgtat tcgctgaaga ttaccgacct cgacccgctg aaatacgcgc
    15961 tgctgttcga gcgtttccta aaccccgaac gcgtttccat gcccgacttt gacgtggact
    16021 tctgtcaggc aaaccgcggc cgcgtgattg aatacgtgcg cgaaaaatac ggcgctcagg
    16081 cggtcagcca gattgtgact ttcggcacga tgtcgtccaa agcggtcatc cgcgacgtgg
    16141 ggcgtgtgct ggaactgccg tttatgctgt gcgacaaact gtccaagctg attccgttgg
    16201 aagccaacaa acccctgagt ttggacgacg cgatgaaggc gcagccgcag attcaggaat
    16261 tgcttgaagc ggaagaagcg gacgaactga ttacgctggc gaaaaaactg gaagacctga
    16321 cgcgcggttt gggtatgcac gcaggcggcg tgttgattgc gccgggcaag atttccgatt
    16381 acagccccgt gtatcaggcg gacgaatccg cctcgcccgt atccatgtac gacaagggcg
    16441 acgtggaaga cgtgggtttg gtgaagttcg actttttggg cctgcgcaac ctgaccatta
    16501 tcgaaatggc gcagaacaac atcaaaaaca ccgccggcga tatcgtcgat gtcggcaaga
    16561 ttccgcttga cgaccagact gcctaccaaa tcttccgcga tgcgaacacc accgccgtct
    16621 tccagttcga gtcgaccggc atgaaaaaaa tgctgaaaac ggcgcatacg accaagtttg
    16681 aagaactcat cgccttcgta tcgctctacc gacccggccc gatggacaac attcccgact
    16741 tcgtcgcacg catgaaggga caggaattcc aatacatcca cccgctgctg gaaggcatcc
    16801 tcgcgccgac ctacggcatt atggtgtatc aggaacaagt gatgcaggcg gcgcagatta
    16861 tcggcggcta ctcgctcggc ggcgcggacc tgctgcgccg cgccatgggt aagaaaaagc
    16921 ccgaagagat ggtgaaacac cgcgaaatct tcgccgaagg tgcggcaaaa caaggcattt
    16981 cgcgcgaaaa atccgacgaa atcttcaact acatggaaaa attcgccggc tacggtttca
    17041 acaaatccca cgccgccgcc tacgccctga tttcctacca gaccgcatgg ctcaaagccc
    17101 actatcccgc cgaatttatg gcggcgacga tgtcgtccga attggacaac accgaccagc
    17161 tcaaacattt ctacgacgac tgccgcgcca acggcatcga gttcctgccg cccgacatta
    17221 acgaatccga ctaccgcttc acgccgtatc cgaacatgaa aatccgctac gcactcggcg
    17281 cgattaaagg cacgggcgaa gccgccgtcg aatccatcat cgccgcgcgg caaagcggcg
    17341 gcaaatttac cggcctgttg gacttctgcg agcgcgtcgg caaagaacac atgaaccgcc
    17401 gcaccctcga agccctgata cgcggcggcg cgttcgacag catcgaaccc aaccgcgcca
    17461 tgctcttggc gaacatcgac cttgccatgg acaacgccga ccaaaaagcc gccaacgcca
    17521 atcagggcgg gctgtttgac atgatggaag acgccatcga accggtgcgg ctcatcgacg
    17581 cacccatgtg gagcgaatcg gaaaaactcg ccgaagaaaa aaccgtcatc ggcttttacc
    17641 tgtccggcca cccgttcggc ccgtatgccc aagaagtccg ccaaatcgcc ccgaccaaat
    17701 taggccgtct gaaaccgcaa gacagcgtgc gcctcgccgg attcgttacc gccgtgcgca
    17761 ccatgatggg caaacgcggc aaaatcgcct tcgtcagcct cgaagattcg agcgggcagg
    17821 ctgaaatcat ggtcggcgga caaacgctgg aaaactgcgc cgactgcctc aaagccgacc
    17881 aagtgctgat tatcgaatcc aaagtcagcc gcgacgatta cggcggcggc gacgggctgc
    17941 gcatcatggc aaaccaagtc atgaccctgc aaacggcgcg cgaacgctac gcccgcagcc
    18001 tcagcctcgc ccttgccccg catcacgaca tcggcggact ggtacggctg ctcgccgccc
    18061 accaactgcc cgacacgccg cgcatcccgc ttcaattgtc gtatgccaac gaaaaagcgt
    18121 cgggcaggct tcaagtgcct ccgaaatgga cggttacacc gagttccgca ttgttcggcg
    18181 aactggaaac attgctcggc agccggtcgg tgcgcgtgaa ttggtaacca aaaatacaaa
    18241 tgccgtctga agcccaaaac cggtttcatt cgtactttat tcgaatgatt gaataaaagt
    18301 aactgccaag aaaaacgtat tttttggtta tttcgccagc ctaaataaat caaccggggc
    18361 gattgatatc cgtgtacatg acacagacag cgccaaaggg aaaaacggca ttttcaaaag
    18421 tatcggtgtc aaaaccgccc tttcattcca aaaataccaa atcgacgaac cgggcaaaga
    18481 aatcagaccg tgccgcctga aaaaacgccc gcccgtccgt taaattaatc tatccctgtt
    18541 tcaggcggcc tgaaacaggg atttttatat caagataaaa tgagaacggg agcgacaacc
    18601 acgggcaaag tcggcgcgaa ctatcaaaaa ttgtagcact gcgaaatgag aacgggagca
    18661 ataaccctta ggtagctctt gttatagcgg attaacgaaa atcaggacaa ggcga
//
LOCUS       DAWTQS010000039        18658 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.039, whole genome shotgun sequence.
ACCESSION   DAWTQS010000039 DAWTQS010000000
VERSION     DAWTQS010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18658
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.039"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            189..635
                     /gene="yciA"
                     /locus_tag="WNA25_001331"
     CDS             189..635
                     /gene="yciA"
                     /locus_tag="WNA25_001331"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFA9824074.1"
                     /translation="MQHEEGNRQRPQGELLLRTVAMPRDTNPNQDIFGGWIMSQMDLG
                     GGILAAEIARGRIVTVAVQEMNFIRPVKVGNVVCCYGHCVRVGNTSLQLKVEVWVKTL
                     MNDCVTEDRHLVTEAVFTYVAIDAEGNPRPIPKEGNPKLSGLLATP"
     gene            complement(686..1507)
                     /locus_tag="WNA25_001332"
     CDS             complement(686..1507)
                     /locus_tag="WNA25_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFA9824075.1"
                     /translation="MAVLITGASAGFGEAMCRTFVGAGYRVIGAARRADRLQALADEL
                     GALFYPLEMDVSCRESVENVLNGIPDEFSDIDCLINNAGLALGLDTADKADFEDWETM
                     IQTNVLGLTFLTRKILPQMVERGGGYVMNLGSIAGNYAYAGSNVYGATKAFVRQFSLN
                     LRAELADKNIRVTNIEPGLCGNTEFSNVRFKGDNERVAGVYEGVEFIRPEDIAETALW
                     LYRRPAHMNVNTIEIMPVAQTFAGMKVIKTAVPEVREDFEKQSMSLFSRIRSWFK"
     gene            1626..2084
                     /locus_tag="WNA25_001333"
     CDS             1626..2084
                     /locus_tag="WNA25_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HFA9824076.1"
                     /translation="MKTQISLAATALALFLSACGNGGAPAQPKGEISENRTAAFKSMM
                     PDFSRMGKMVKGEEPYDVEKFKQAAAAFAESSKKPFTLFESDPQGNGRALPAVWSDGA
                     KFEAEKTKFAAAVEKLNAAAQTGKLDEIKAAYGETGASCKSCHDSFRAPE"
     gene            complement(2227..3363)
                     /gene="lst"
                     /locus_tag="WNA25_001334"
     CDS             complement(2227..3363)
                     /gene="lst"
                     /locus_tag="WNA25_001334"
                     /EC_number="2.4.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyllactosaminide
                     alpha-2,3-sialyltransferase"
                     /protein_id="HFA9824077.1"
                     /translation="MGLKKVCLTVLCLIVFCFGIFYTFDRVNQGERNAVSLLKDKLFN
                     EEGKPVNLIFCYTILQMKVAERIMAQHPGERFYVVLMSENRNEKYDYYFNQIKDKAER
                     AYFFYLPYGLNKSFNFIPTMAELKVKSMLLPKVRRIYLASLEKVSIAAFLSTYPDAEI
                     KTFDDGTNNLIRESSYLGDEFYVNGAIKRNFARMMVGDWSIAKTRNASDEHYTIFKGL
                     KNIMDDGRRKMTYLPLFDASELKAGDETGGTVRILLGSPDKEMKEISEKAAKNFNIQY
                     VAPHPRQTYGLSGVTALNSPYVIEDYILREIKKNPHTRYEIYTFFSGAALTMKDFPNV
                     HVYALKPASLPEDYWLKPVYALFRQADIPILAFDDKNQSHGKSK"
     gene            complement(3354..3614)
                     /locus_tag="WNA25_001335"
     CDS             complement(3354..3614)
                     /locus_tag="WNA25_001335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824078.1"
                     /translation="MAALFALCSILACLNAAAGKEGRNGFPAFGGGVGGVVPDVRRHI
                     FGKIDFNIRQLSEYLLKFRIFRTGTLGAYVQFVGMEFLGIWG"
     gene            3693..5918
                     /locus_tag="WNA25_001336"
     CDS             3693..5918
                     /locus_tag="WNA25_001336"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFA9824079.1"
                     /translation="MTQKSTIVYTHTDEAPALATQSLLPIVQAFARHADIDVKTADIS
                     LSGRILAAFPEYLTEAQRVPDALAELGELVKQPGANVIKLPNISASVPQLTAAIKELQ
                     SKGFAVPDYPADPQTDEEKAVRERYDRIKGSAVNPVLREGNSDRRAPKAVKNFAKKNP
                     HSMGAWAKDSKTHVATMQSGDFFHNEQSVTVPDATSVSIVFTDKQGNKKELREPVALK
                     AGEIIDAAVMSKKALLAFLAEQVKDAKAKGVLFSLHMKATMMKVSDPIIFGHAVKVFF
                     APVFEKFGGKLAAAGVNVNNGFGNLIANLDKLDADTRAAVEAEIAAVYAANPDLAMVD
                     SDKGITNLHVPSDVIVDASMPAMIRNSGRMWDKNGKAQDTKAVIPDSSYAGVYQATID
                     FCREHGAFDPTTMGTVPNVGLMAQAAEEYGSHNKTFEIEADGQVQVIDAAGNVLMQHD
                     VEAGGIWRMCQTKDAPVKDWVQLAVNRARLSNTPAVFWLDENRPHDKSLLAKVKAYLA
                     ELDTNGLDIRVLAPEEAAKFSLGRLKNGEDTISVTGNVLRDYLTDLFPILELGTSAKM
                     LSIVPLMNGGGMFETGAGGSAPKHVQQFLEENHLRWDSLGEFLALAVSFEHLAQKTGN
                     AKAQVLADTLDAATEKLLLNDKSPKRKAGELDNRGSHFYLTLYWAQELAAQDKDAELK
                     AAFAPLAAALTADEAKIVEELSAVQGKAVDIGGYYAANPEKAAQAMRPSATFNQVLNA
                     L"
     gene            6215..6517
                     /locus_tag="WNA25_001337"
     CDS             6215..6517
                     /locus_tag="WNA25_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824080.1"
                     /translation="MKTVPTILTGILLATALPASAHGMHKSKPLAMDELPPICQQYFK
                     RTETCYNKAGNKADFARNNTKFLFQALPAADLGQRKQMCQIAMDSFAEKTRNLNCE"
     gene            7476..7643
                     /locus_tag="WNA25_001338"
     CDS             7476..7643
                     /locus_tag="WNA25_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824081.1"
                     /translation="MVNEKLAENRKRYEQKRVIKKVSFNAETEKELLEYAQNLDFSQW
                     VKSIIKEKIKK"
     gene            7803..8654
                     /locus_tag="WNA25_001339"
     CDS             7803..8654
                     /locus_tag="WNA25_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFA9824082.1"
                     /translation="MNAVQVLNFQQNSVRTVADNKGELWFLANDVCEILGYTNPRRTV
                     DLHCKSRGVTKRYTPTASGEQEMTYINEPNLYRLIIKSRKPAAEAFEEWVMETVLPAI
                     RKTGGYQITPKTTADDRTGLRQAVAALVGRKGIDYYSAYSMIHQRFNVESVEGIPAGK
                     LPEAVAYVHALTLHTGLTGEVLDREPLPAPQPALPIDGNALAGIAAMVYYGTWMIELG
                     KDISAPLKQLGNRQAVTMWTVWHETRSILKRSAAALEVLRGYADKDTSDRIAACLEGI
                     YRKAAAR"
     gene            complement(8840..9514)
                     /locus_tag="WNA25_001340"
     CDS             complement(8840..9514)
                     /locus_tag="WNA25_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824083.1"
                     /translation="MNLKALIARFRVLANDKAEPYFWSDEEVSGWLNDAVHEACLRGR
                     LLHSDDAFVTDVEKGRPLYAYAAGGFAAGYAYEIDSIRFVSDGKPVCLKLVSPEAADV
                     CAPGWRDGAQTGLPVYAVQGDGKLTLAPAPDRDGRLFAGGYCLPRDMAGDGDEPEINS
                     IHHRNLVYWALAEAFSIPDAETFDPQRSESARRRFELYFGLPADSDLRRITREDTPHL
                     NRHFWI"
     gene            complement(9511..9996)
                     /locus_tag="WNA25_001341"
     CDS             complement(9511..9996)
                     /locus_tag="WNA25_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824084.1"
                     /translation="MKRRPGICLCGVAVFDNAVRTAAGGWMDKVFIKYIGGRAVWRDR
                     IYHTGLVFEDGQVREVSAGAAAKLLRHGDVFAAVPGKRVEKADDTEALEKAGASELER
                     ETAAFDAVQDVILQINRMGKDELELYAKANYGQGLDKRKSAENLRGDVVRMVRQFGIA
                     Q"
     gene            complement(10002..10475)
                     /locus_tag="WNA25_001342"
     CDS             complement(10002..10475)
                     /locus_tag="WNA25_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824085.1"
                     /translation="MAEIYVKNNGGNRFGGVPYGNLAAEHYRIVAKQDGAILGADASE
                     PPKAGDVLALGVLEQGFRLDDAQIIVKTAMSSGITADVGFAYADGADDAKVPQDAAYF
                     ASGADFASAGRIRCQSAKLVTLPKQALLTVTLKGAENKKAADIDILIYGEKFGQL"
     gene            complement(10530..11825)
                     /locus_tag="WNA25_001343"
     CDS             complement(10530..11825)
                     /locus_tag="WNA25_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4043 family protein"
                     /protein_id="HFA9824086.1"
                     /translation="MAQKTNTAYGDPQAMMKQAAGLFAMHMQRNSTLNRLAGKMPAGT
                     AGAEATLRKQTTQHMPVVRCQDLTRGMGDEIRFNLVNPVSALPIMGDNTAEGRGVGMS
                     LSEAGLRVNQARFPVDGGGTMTNQRSPADYRALIRPAAQSLMDRYADQTLLVHMAGAR
                     GFHDNIEWGVPLAGDPKFNDYAVNPVKAPSKNRHFTASGDAVTGVGDNGGELKIASTD
                     LFTMDTVDSMRTVLDQIPLPPPIVKFEGDKAAGDSPLRVWLLSPAQYNRFAADPKFRQ
                     LQASAIARASQANQNPLFLGDAGLWNGFILVKMPRPIRFYAGDEMKYCADKFSEAESG
                     LKIPASFADKFAVDRSVILGGQAVLEAFANTGKHGGMPFFWSEKELDHGNRVETLVGT
                     IRGVAKTRFAVDVGGGAKEITDYGVTVVDTVVPLHGGIR"
     gene            complement(11846..12368)
                     /locus_tag="WNA25_001344"
                     /pseudo
     CDS             complement(11846..12368)
                     /locus_tag="WNA25_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146864.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12461..18367)
                     /locus_tag="WNA25_001345"
     CDS             complement(12461..18367)
                     /locus_tag="WNA25_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLxRFG domain-containing protein"
                     /protein_id="HFA9824087.1"
                     /translation="MPDGLARQYADMAAKYRAKPSEAVGIDPDDGAVSAAAALAADSG
                     AAVPSAVSDDMEARSVADDAPSGRSADADRGGVPSAYGNVRPGGAPRGAASVAPGGSA
                     AAASGGIARVSPLPAGQYFDGLDTRGRKALAKEAGLDIKGVADFGQIAAPVRRKIEQA
                     YHARIEADYQAASEAKQGYLPPPVRMADAVPVPKKGFSVPADALDKESRRRFDALPEW
                     VRRHAQTVADYTADGIMRREAGMADMRGHYPEGLAESAGAVRAYRAQHPESADVLDRL
                     NRAVYGYRRNNGWSVPLLSREGERLQGVRTALPDDGASEAVVGGGRGLTRALPTEDKG
                     LAQDVRQDVRQGLTQGGRGLTPDAGADANAAALQGLPGSAVASGNAPARRQNLQVRAR
                     AEGAAPGLSASENLAGTDGGKRAPVAGKRPDTVLPVLNPQVAASAGRVSPKKRMADAA
                     ADRRRPEKAGVPLGGGEYRFEHTDRRHIDALAGVPGRPGKGGMPEEFADMAGPSNSDG
                     LVSDGRRYLKGREAETLRAGGLSEAVPSEPGRDYRPTQEARAPARVMARPRDAAADGK
                     PAGRAQPARAKDTPVAGKAAAAKNAATEKPSSDKVRNIESGKSHFDGGKGKSAAAQGA
                     ATEKPSSDKAAKPETFAKTASDNPEEARRKARVLQGGPVYTVKERQAPQGFKALREHA
                     ENIKKRLAESIGGLAERVDVAAVSETAPDKAQMLLSQRVEGWFDGRTGKITLVAENLT
                     PERAVWAAWHELGHRGFAADGFAKYREELERADGNGLIRRIADAVQEGREGTGDAAAS
                     VRSAAVEEAVAELYAAQRTGGWAGIENRYGVKVGNGLKRGIAGVLARIGALLRRVLQR
                     LAGKAGGAMSDADVFAMLADLHGNVEGARDAPWDGNHRAVMFARAEDGAAERSKSESL
                     EKLRRAETIRISGREVPEGGNLREYKRNALEYGKSLRGPYVNKDTGREISLGRSGITE
                     ILRHDYKDAEHLQSIAAIPQIIENAVYIDTLPNEDLAKNGDIQGYEYYVSGLNVGGAD
                     YTVRAAVAVSRNGNRYYDHKLTKIEKGNLLSLLDRVSTTGASESKSPLSGIDDKRLLQ
                     ILQDKDAGKGGIADFDTEAVRFSRAANIGAAISRITGKKSDLRNALKDRWDASKGIQL
                     QFLGRRQIEDIYGGVLDGLKEYGRLSELFGADANKAVTEADKVVREWGRLKEEDAKAL
                     ADLMHDATLAKVDADPLMRKDARGRLDGIRTALDIADGKIAKARAAVASAGARTARAD
                     AAYNKAQRAADKAAYALEKAQEKHGREILADEADMRLRRLFYADSEAKRALRRAGADA
                     AAESRAKTDAVRMLEQARADVKRLEKDEVGAQKALEGLALLNRRFAGLPDAAQRVYRK
                     ARDDYRAHFGQVRDALAERLARAGQDAETVRRLKERFDNELGGVYFPLARFGDYLVVV
                     KDADGNSANVSRAETLSEAEKLRDALKADFGPGFKVSPVMKSRDYIRSRDAVGSGFMK
                     ELGEAVGRMDLDPAQRARLNDTLTQLYLNSLPDTSWAKHGIHRKGVPGFSDDARRAYA
                     QNMGSGANYLAKLRYADRMAEQLDVMQDFVDGRKYEEGFDQRQLQRVADEMRKRHEAV
                     MNPNPSKLAQALTGFGFLWMMGMSPASAVVNLSQTAMVAYPVMAAKWGYAGAARELLR
                     ASKQIGLRFGEKFNTIEDSLNGDEKAAFRKAADYGVIDLSQAHDLAGVANGDPGLAGS
                     AWQKVMDKAAWLFHHAEKFNRQVTFVAAYRLAKRAGADSEAAFEQAKKATYDGHFDYA
                     AQNRPRFMMGNAAKVVFLFKQYSQNILYALGRNAYLAFKGDKEARKTLAGLLVSHAMA
                     SGILGLPFVSTLLAVASMLGSDDDDPWDAEAALRNMLADTFGDKAGEVLAKGFSRLTP
                     LDVSGRLGLDQLVFPDIQDGLEGKKWAESLVVGSTGAVVGAGIGAADGVRTRSSVPRT
                     ANTLSLMKSW"
BASE COUNT         4383 a         6160 c         4310 g         3805 t
ORIGIN      
        1 tagggtagga tttggcggga ttggtgggct gaagcccgcc ctacgcatcc gccctacaat
       61 actccttgcg aatctgtccc gcgcccgacc ttgtatgtct caaatgggtt aaactacgca
      121 cttcccgact tcccgtttcg ggcaggggtg caggtccgat ttatatttca ataaaacaag
      181 gaaactttat gcagcacgaa gaaggcaacc gccaacgccc tcaaggcgaa ctgctcctgc
      241 gtaccgtcgc tatgccgcgc gataccaatc ccaaccaaga cattttcggc ggctggatta
      301 tgtcgcaaat ggatttgggc ggcggcatat tggcggcgga aatcgcgcgg gggcgcatcg
      361 ttaccgtcgc cgttcaggaa atgaacttca tccgcccggt caaggtcggc aacgtcgtct
      421 gctgctacgg gcattgcgtc cgcgtgggca atacttccct ccagcttaaa gtcgaagtct
      481 gggtgaaaac tttgatgaac gattgcgtta ccgaagaccg ccatctcgta accgaggcgg
      541 tgttcactta tgtcgccatc gatgcggaag gcaatccgcg cccgattccg aaagaaggca
      601 accccaaatt gtcaggctta ttggccaccc cgtaagaata cccgtaaaaa tgccgtctga
      661 aactgtcttc agacggcatt ccgtatcatt tgaaccaaga cctgatgcgg gaaaacagcg
      721 acatactctg tttttcaaag tcttcccgca cttcgggcac ggctgttttt atgaccttca
      781 ttcctgcaaa agtctgcgcc acaggcataa tttcaatcgt gttcacattc atatgcgccg
      841 gccggcggta cagccacaat gcggtttccg caatatcttc ggggcggata aattccacac
      901 cctcatacac gcccgccacc ctctcgttat cgcctttgaa gcgcacgttg gaaaactccg
      961 tattgccgca caaacccggc tcgatattgg taacgcggat gttcttatcc gccagctctg
     1021 cgcgcaaatt caggctaaac tggcgcacaa acgccttggt cgccccgtaa acgttgctgc
     1081 cggcataagc ataattgcct gcaatcgaac ccaaattcat cacataaccg ccgccgcgtt
     1141 ccaccatttg cggcaaaatc ttgcgcgtca ggaacgtcaa acccaaaaca ttggtttgaa
     1201 tcattgtttc ccaatcttca aaatccgcct tgtccgccgt gtccaaaccc aaagccagcc
     1261 cggcattgtt gatgaggcag tcgatgtcgg aaaattcatc ggggatgccg ttcaagacgt
     1321 tttccaccga ctcgcggcac gacacgtcca tttccaaagg gtaaaacaaa gcacccaatt
     1381 catccgccaa agcctgaagc ctgtccgcac ggcgcgccgc accgataacg cggtatcccg
     1441 ccccgacaaa cgtgcggcac atcgcttcgc cgaagcctgc cgaagcaccg gtaattaaaa
     1501 cagccattgt ttttcctttc tttctcgttt cccaaacctg tttcaggtat cataacaccc
     1561 ttcagacggc atcgcgccgg ctgaaaacga caacaaccgt accaccacga caggacacat
     1621 tcaacatgaa aacccaaatc agccttgccg caaccgccct cgccctattc ctttccgcct
     1681 gcggaaacgg cggcgcgccc gcacagccca aaggcgaaat ttccgaaaac cgcaccgccg
     1741 cgttcaaatc tatgatgccc gacttctcgc gtatgggcaa aatggtcaaa ggcgaagagc
     1801 cttacgatgt cgaaaaattc aaacaggcgg cggcggcgtt tgccgaaagc agcaaaaaac
     1861 cgttcacact ttttgagtcc gatccgcaag gcaacggccg cgccctgcct gccgtttggt
     1921 cggatggtgc aaaatttgaa gccgaaaaaa caaaattcgc cgccgccgtc gagaaactca
     1981 acgccgccgc ccaaaccggc aaactggacg aaatcaaagc cgcctacggg gaaaccggcg
     2041 caagctgcaa atcctgccac gacagtttcc gcgcaccgga ataacatccg aaacaaaaca
     2101 atgccgtctg aagccttcag acggcatttt ctttgtcatt tcggcacacg caaagcaatc
     2161 aaaaattatc gtcaattcaa aaaatacgcc atatcctgat ttttgcctta cttactccgc
     2221 cattttctat tttgatttac catgcgattg atttttatcg tcaaatgcca aaatcggaat
     2281 gtcggcctga cggaacagcg cataaacggg cttgagccaa taatcttccg gaagggaagc
     2341 cggtttcaat gcgtaaacgt gcacattggg aaaatccttc atcgtcaacg ccgcaccgct
     2401 gaaaaaggta taaatttcat acctcgtatg cgggtttttc ttaatttcgc gcaaaatata
     2461 gtcttcgatg acatagggcg aatttaacgc ggttacgccg gaaagcccgt aggtctggcg
     2521 gggatgcggc gcgacatatt gtatgttgaa attttttgcc gccttttccg aaatttcctt
     2581 catctctttg tcgggcgaac ccaaaagtat ccgcaccgtg ccgcccgttt cgtcccccgc
     2641 cttcagttcg gacgcatcga acagcggcag gtaagtcatc ttgcggcggc cgtcgtccat
     2701 aatgtttttc aaacccttga atatcgtgta atgctcgtcg gaagcattgc gggttttggc
     2761 gatgctccaa tccccgacca tcattcgggc aaaattccgc ttaatcgccc cgtttacata
     2821 aaactcatcg cccaaatagc tgctctcccg tatcaggttg tttgtgccgt cgtcaaaggt
     2881 cttgatttcc gcatccgggt aagtactcaa aaaggcggca atactgactt tttccaaact
     2941 cgccaaataa atccgcctga ccttcggcag cagcatcgac ttcaccttca gctccgccat
     3001 cgtcggaatg aaattaaacg atttgttcaa accgtagggc aggtagaaaa aatacgcccg
     3061 ctccgcctta tcctttatct gattgaaata ataatcgtat ttttcattcc tgttttcaga
     3121 catcagcacc acataaaacc gctcccccgg atgctgcgcc ataatccttt ctgccacctt
     3181 catctgcaat atggtatagc agaaaatcag attgacgggt ttcccctctt cattgaagag
     3241 tttgtccttc agcagggaaa ccgcgttcct ttccccctga tttacccggt caaacgtata
     3301 aaatatcccg aagcaaaaaa caatcaggca caacacggtc aaacagactt ttttcaaccc
     3361 catatcccta aaaactccat tccgacaaat tgaacatacg ccccgagtgt cccggtgcgg
     3421 aaaatgcgga attttagcag atattccgac agttgccgaa tattaaaatc aattttaccg
     3481 aaaatgtgcc gccgcacatc aggcacaacg ccgccgacgc cgccgccaaa cgcgggaaag
     3541 ccatttctcc cttcctttcc cgccgccgcg ttcaaacacg ccaagatcga acaaagcgcg
     3601 aacaaagccg ccaaaatatg ccataatagc cgcgcacatt ccccgcgcaa tgtgtttcaa
     3661 aagaaaaaac caacctacaa gagaataatc ctatgactca aaaatccacc attgtttata
     3721 cccataccga cgaagccccc gcgctggcga cccaatcgct gctgccgatt gtgcaggcgt
     3781 ttgcccgcca cgccgatatt gatgtcaaaa ctgccgacat ttccctgtcc ggccgcattt
     3841 tggcggcatt ccccgaatat ctgaccgaag cgcagcgcgt acccgacgcg cttgccgaat
     3901 tgggcgaact ggtgaaacaa cccggtgcaa acgtaatcaa actgccgaac atcagcgcat
     3961 ccgtacctca actgaccgcc gcgattaaag aattgcagtc taaaggcttt gccgttcccg
     4021 actatcctgc cgaccctcaa accgatgaag aaaaagccgt acgcgaacgc tacgaccgca
     4081 tcaaaggcag cgcggtaaac cctgtcctgc gcgaaggcaa ctccgaccgc cgcgcgccta
     4141 aagcggtgaa aaactttgcc aaaaaaaatc cgcacagcat gggcgcgtgg gcgaaagact
     4201 ccaaaaccca cgttgccacc atgcaaagcg gcgacttttt ccataacgaa caatccgtta
     4261 ccgtacccga tgcgacttcc gtatccatcg tgttcaccga caaacaaggc aataaaaaag
     4321 agctgcgcga gcctgttgcc ctgaaagccg gcgaaatcat tgacgcggcc gtaatgagca
     4381 aaaaagccct gctcgccttc cttgccgaac aagtgaaaga cgcaaaagct aaaggcgtat
     4441 tgttctcgct gcatatgaaa gccacgatga tgaaagtgtc cgacccgatt atcttcggac
     4501 acgccgtcaa agtgttcttc gcgcctgtat ttgaaaaatt cggcggcaaa ctggctgccg
     4561 caggcgtcaa cgtcaacaac ggcttcggca acctgattgc caatctggac aagctggatg
     4621 cggacacccg cgccgccgtc gaagctgaaa tcgccgccgt ttacgccgcc aaccctgatt
     4681 tggcgatggt cgattccgac aaaggcatca ccaacctgca cgttcccagc gatgtcatcg
     4741 ttgatgcttc tatgcctgcg atgatccgca attccggccg tatgtgggac aaaaacggca
     4801 aagcgcaaga caccaaagcc gtgattcccg acagcagcta tgccggtgtt taccaagcaa
     4861 ccatcgattt ctgccgcgaa cacggcgcat tcgatccgac aacaatgggt actgtgccca
     4921 acgtcggact gatggcgcaa gcggcggaag aatacggctc acacaacaaa acctttgaaa
     4981 tcgaagccga cggtcaggtt caagtgattg atgcggcggg caatgtcctg atgcagcacg
     5041 acgttgaggc aggcggcatc tggcgcatgt gccaaaccaa agacgctccc gtcaaagact
     5101 gggtacaact cgccgtcaac cgcgcccgcc tgagcaacac gcccgccgtg ttctggctcg
     5161 acgaaaaccg tccgcacgac aagagtctgc tcgccaaagt caaagcctac cttgccgaat
     5221 tggataccaa cggcctcgac atccgcgtcc tcgcgcccga agaagccgct aaattcagct
     5281 tgggccgtct gaaaaacggc gaagacacca tttccgtaac cggcaacgtc ctgcgcgatt
     5341 atctgaccga cttgttcccg attttggaac tgggtaccag cgcgaaaatg ctgtccatcg
     5401 ttccattgat gaacggcggc ggtatgtttg aaaccggcgc gggcggttct gcaccgaaac
     5461 acgtccaaca gttcctcgaa gaaaaccacc tgcgttggga ctcattgggc gaattcctcg
     5521 cgcttgccgt atcgtttgaa catctggcgc aaaaaaccgg caacgccaaa gcccaagtcc
     5581 tcgccgacac tttggatgcc gccaccgaaa aactgctgtt gaacgacaaa tcgcccaaac
     5641 gcaaagcggg cgaactcgac aaccgcggca gccatttcta cctcaccctc tactgggcgc
     5701 aagaattggc ggcgcaagac aaagatgccg aactgaaagc cgcatttgct ccattggcag
     5761 ccgctttgac tgccgacgaa gccaaaatcg ttgaagaact ctctgccgta caaggcaaag
     5821 cggtcgacat cggcggctac tacgcagcca atcctgaaaa agccgcacaa gcgatgcgtc
     5881 cgagcgcaac ctttaatcaa gtactaaacg ccttataagc acaaaggcaa aaatgccgtc
     5941 tgaacattcg tcgttcagac ggcattagtt atccctatct gcctgattat gattaaacgc
     6001 cgcacccatt ggatatatag tggattaaca aaaaccagta cggcgttgcc ccgccttagc
     6061 tcaaagagaa cgattctcta aggtgctgaa gcaccaagcg aaccggttcc gtactatttg
     6121 tactgtctgc ggcttcgtcg ccttgtcctg atttttgtta atccactata aatcatataa
     6181 tctttagttt tttaccctac ggaaagacaa taatatgaaa actgttccaa ccatcctgac
     6241 cggcatcctt ttggcaaccg cccttcccgc atccgcacac ggtatgcata agagcaaacc
     6301 cctggctatg gacgagctgc cgccgatttg ccaacaatat ttcaaacgca ccgaaacctg
     6361 ttacaacaaa gccggaaaca aagcagattt cgcacgcaac aacaccaaat tcctattcca
     6421 agccctacct gccgccgatt tgggacagcg taagcaaatg tgccaaatcg ctatggattc
     6481 gtttgcagaa aaaacccgca atctgaattg cgaataaagc cgcatcagca atagcaatgc
     6541 cgtctgaaac aaaggttcag acggcattct cacatcaaaa cgtaccggct gataaaaccc
     6601 gccttccccg catccctgac ccgaacagga ttaaacggag taacggtttc ggccattatg
     6661 gcaagtcccc atcccaaaaa tgaccatata aattaatacc ataaaacatt gataaaaagc
     6721 gtctgccggt tgcggtttgt tgcagggcgt tacggaaaat catcgtaaac cgatgatttt
     6781 tatatagtaa ttgacacaac ctgaacaaaa aagaaccgcc ctgaatcagg gcggttttgc
     6841 tttgcggcgg aaacggcagg attcgactaa attttatttt attgatttaa atacatttat
     6901 ttcttataaa aatttaattt accataaaaa cagccatata caaaaatctt ggagtaacta
     6961 ctgcattaca ctattagaag gatgcagggt catgtaatat ttaattaaat taatatataa
     7021 attaaatatt tgaattatat ttataaaaaa aggtgcaaat aaggtgcaaa taaaattagt
     7081 ttttcccaat gggttaaaga acaaatccga aaagagttct taaaatagcc cgaccgccgc
     7141 caagccgcaa acttggcggc ttctttatgc cgtccgaaag cccttcagac ggcatttttc
     7201 tttgattttg ttgggggaat ggttgacttt gaacgtgtcc acgtttataa ttcaattcat
     7261 cggcgcatcg ggtgccgata cgggaaaagc cccaccgaag cggggcttgt acagggagta
     7321 ggaaaatgaa gctcatcctt caaattctca tcctggtttt aatcttgatg ctggcaggcg
     7381 atacgcctat tagcggataa aaccggctgg ggcggtagcc gccgccccag tccctatctt
     7441 acaaaaggca gttcaaaaaa tcaaggagtt tgcctatggt taatgaaaaa ctggcagaaa
     7501 accgaaagcg ttacgagcaa aaacgagtca ttaaaaaagt ctcgtttaat gcggaaacag
     7561 aaaaagagct tttggaatat gcccaaaatc tcgatttttc ccaatgggtt aaatcaatca
     7621 taaaagaaaa aatcaaaaag taattgactt tgaacgtgtc cacgtttatt attcttacaa
     7681 cggcacagcc gttaatggaa aaccgaacca agcgggaact tggttcggtt aagtgaaaag
     7741 cagttctagg aaaactcttt tcgcgtcttt taaataacct ctaaaagaaa gactattcta
     7801 tcatgaatgc tgtccaagtt ttaaactttc aacaaaactc cgtccgtact gttgccgata
     7861 acaaaggcga gttgtggttt ttggcaaacg atgtttgcga gattttaggc tacaccaacc
     7921 ctcgccgaac ggttgacctg cactgcaaaa gcaggggcgt aacaaaacgg tacaccccca
     7981 ccgccagcgg cgaacaggag atgacctata ttaacgagcc gaatctctac cgcttgatta
     8041 tcaaatcccg caagcctgcc gcagaagcgt ttgaagaatg ggtaatggaa accgtcctgc
     8101 ccgccatccg caaaacaggc ggctaccaaa ttactcccaa aaccaccgcc gacgaccgta
     8161 ccggattgcg ccaagctgtt gccgcgcttg tcggacgcaa aggcatagac tactactccg
     8221 cgtacagtat gatacaccaa cgcttcaacg tcgaatccgt cgaaggcatc cccgccggga
     8281 agctgcccga agccgtcgcc tacgtccacg cattgacact gcacacgggc ttgacgggcg
     8341 aagtccttga ccgcgaaccg ttacccgcgc cgcaacccgc cctgcccatc gacggcaacg
     8401 ctttagccgg cattgccgct atggtttatt acggcacatg gatgattgaa ttgggcaaag
     8461 acatctccgc gccgctcaaa cagcttggca acaggcaggc ggttacgatg tggacggttt
     8521 ggcacgaaac ccgttcaatc ctgaaaagat ccgccgcggc cctcgaagtg ttgcggggat
     8581 atgcggacaa agacacctcc gaccgcatag ccgcatgtct tgaaggcatt tacagaaagg
     8641 ctgcggcaag gtaaacggaa aatgccgtcc gaaccttcgg acggcatttt tcagacggca
     8701 tagcgtttca acatttcccg ataatccgcc ggttcccgtc cggcaaattc aaaggccttc
     8761 aaatcgccgc cgcccgcatt tcggcgcccg tccggccggc aggcggtttg tttttcacaa
     8821 aatccttcgg cgtccgccgt caaatccaaa aatgcctgtt caggtgcggc gtatcctcac
     8881 gcgtgatacg gcgcaaatca ctgtcggccg gcaggccgaa atacagctcg aaacgcctcc
     8941 ttgccgattc cgaacgctgc gggtcgaaag tctccgcatc cgggatactg aaagcctccg
     9001 ccaaagccca ataaaccaaa ttccgatgat gtatggaatt gatttccggc tcgtccccgt
     9061 cccccgccat atcccgcggc aggcaatacc ccccggcaaa caacctgccg tcacggtccg
     9121 gcgcaggcgc aagcgtcagc ttcccgtcgc cctgcaccgc ataaaccggc agccccgttt
     9181 gcgccccgtc gcgccaaccc ggggcgcaaa catccgccgc ctccggcgaa accaacttca
     9241 ggcacaccgg cttgccgtcg gacacaaaac ggatactgtc gatttcatac gcataacccg
     9301 ccgcaaaccc ccctgccgca taagcataaa gcggccgccc tttttccaca tccgttacaa
     9361 acgcatcgtc cgaatgcagc agccggccgc gcaggcacgc ctcgtgaacc gcatcgttca
     9421 accatcccga cacctcttcg tcgctccaaa aatacggctc ggccttatca ttggcaagca
     9481 cgcggaaacg cgcaatcaaa gccttcaaat tcattgcgcg ataccgaact gacgaaccat
     9541 ccgaaccacg tccccgcgca aattctccgc cgatttgcgc ttatccaaac cctggccgta
     9601 attcgccttc gcatacagtt caagctcgtc cttgcccatc cggttgattt gcaggatcac
     9661 atcctgcacc gcgtcaaacg ccgccgtttc ccgttcgagt tccgaagcgc ccgccttttc
     9721 caaagcctcc gtatcatccg ccttttccac gcgtttgccc gggacggcgg caaacacatc
     9781 gccgtggcgc aacaactttg ccgcagcccc ggccgacacc tcgcgcacct ggccgtcttc
     9841 gaaaaccaag cccgtatgat agatacggtc gcgccacacg gcacgcccgc cgatatactt
     9901 gataaaaacc ttatccatcc aaccccccgc cgccgtccgg acggcattgt caaatacagc
     9961 aacgccgcaa aggcaaatcc ccgggcggcg tttcattccg cttacaactg gccgaacttc
    10021 tcgccgtaaa tcaaaatatc gatatcggcg gccttcttat tttccgcccc tttaagcgta
    10081 accgtcagca aagcctgctt gggcagcgta accagtttgg cagactggca gcggatacgc
    10141 ccggcagacg caaagtccgc gcccgaagca aaataagccg catcctgagg cactttcgca
    10201 tcatccgcac catccgcata cgcaaagccg acatcggcag tgataccgga agacatcgcc
    10261 gtcttcacaa taatctgcgc gtcatccaaa cggaaaccct gttccaagac acccagcgcc
    10321 aacacatcgc ccgccttagg cggctcagaa gcatccgccc ccaagatcgc accgtcctgc
    10381 ttcgcaacga tacggtaatg ctccgccgcc agattgccgt aaggcacgcc gccgaaacgg
    10441 ttgccgccat tgtttttcac ataaatctca gccataaaaa acctcaaccc aacaaatagg
    10501 aaaagccgcc ctttcaagcg gcattcgaat cagcggatac cgccgtgcaa aggaaccacc
    10561 gtatccacaa ccgtgacacc gtagtcggta atttccttcg cgcccccgcc gacatccacg
    10621 gcaaagcgcg ttttcgccac accgcgtatc gtaccgacga gcgtttccac acggttgccg
    10681 tggtcaagct ccttctcaga ccaaaagaaa ggcataccgc cgtgtttgcc ggtattcgca
    10741 aacgcctcca acaccgcctg gccgcccaaa ataaccgaac ggtcgaccgc aaacttgtcc
    10801 gcaaacgaag ccgggatttt caagcccgat tccgcctcgc tgaacttatc ggcgcaatac
    10861 ttcatctcat cgcccgcata gaaacggatg gggcgcggca ttttcaccag gataaagccg
    10921 ttccacaaac ccgcatcgcc caaaaacagc ggattttgat ttgcctggga ggcgcgcgcg
    10981 attgccgaag cctgaagctg gcggaatttc ggatcggcgg caaaacggtt gtactgcgcc
    11041 ggggaaagca gccacacgcg caaaggcgaa tcacccgccg ccttgtcgcc ctcaaacttc
    11101 acaatcggcg gcggcagcgg aatctggtcg agcacggtac gcatactgtc caccgtatcc
    11161 atcgtaaaca aatcggtaga ggcaatcttc aactcgccgc cgttgtcccc aacgcccgtt
    11221 accgcatcgc ccgaagccgt aaaatggcgg tttttggacg gggctttgac cggattgacc
    11281 gcataatcat tgaatttcgg gtcgcccgcc aaaggcacgc cccattcgat attgtcatga
    11341 aaaccgcgcg cgcccgccat atgcaccaac agcgtctggt cggcataacg gtccatcagg
    11401 ctttgcgccg ccggacgaat cagcgcgcga taatcggcag ggctgcgctg attcgtcatc
    11461 gtgccgccgc cgtcaacggg gaaacgcgcc tgattcacac gcaaacccgc ctccgacagg
    11521 ctcatcccca cgcccctgcc ttccgccgtg ttgtcgccca taatcggcag ggcggaaaca
    11581 gggttgacca aattgaaacg gatttcgtca cccatgccgc gcgtcaaatc ctgacagcgc
    11641 acgaccggca tatgctgggt cgtctgtttg cgcaaagtcg cctccgcacc cgcggtaccg
    11701 gcaggcatct tgcccgccaa acggttcagc gtactgttgc gctgcatatg catcgcaaac
    11761 agccccgccg cctgcttcat catcgcctgc gggtcgccgt aggccgtatt cgtcttttgt
    11821 gccataaaga gcctccctca aaaactcaag aatttaaaaa cttctcaacc tgctcttccg
    11881 tcatggaagc cagtttttcc gccagcgcgt tgccgctcaa ataatccggc gggccgtccg
    11941 aagccgtatg gtcgcgcccc gccggaatat ccgacagcgt attgggcgta ttcttcccgg
    12001 caggcggctt ttcggcgggc ttttccggtt cggcggcgga ttttgccgcc ttaaacatat
    12061 cgaacgcccc gatgacctcg gcggcagtcc cgtccttaag cgcgtcatta aacgccttgc
    12121 ggacaagcgg gttctgcgca tccagccact cctccagctc gcggctttcc acaatcgaat
    12181 ccgcatcggg gtgcgccgta tagatttcgc ggtaatgcgc ctttgccgcc tcctgccgcc
    12241 tcctgccgct tcatgtccgc ctcataaccc gccagcttct cctgaatcag cttttccacg
    12301 ccctttttca catcctcgtc ggaaaaatcc ccgaacaacg aattatcgga accgtcagcc
    12361 gtttccactc cgggtttttc cgcccccttc tcaagctggg caatgcgctc cctcaaggct
    12421 tgggcttcgg ctttcgccga cttttccgcc tcgcgcgcct ctaccagctt ttcataaggg
    12481 atagtgtgtt tgccgtcctt ggcacggatg accttgtccg cacgccgtcc gccgcgccga
    12541 tacccgcgcc gaccaccgcg cccgtactgc cgaccaccag cgattccgcc cacttcttac
    12601 cctccaaacc gtcttggata tcggggaaaa ccaactggtc caaacccaaa cgccccgaca
    12661 cgtccagcgg cgtcaggcgg ctgaagccct tggccaacac ctcgcccgcc ttatccccga
    12721 aagtgtccgc caacatattg cgcaacgccg cttccgcatc ccacgggtca tcgtcgtcac
    12781 tgcccaacat cgaagccacc gcaagcagcg tcgacacaaa cggcagcccc aagatgcccg
    12841 aagccatcgc atgcgagacc aacagccccg ccagcgtctt acgcgcctcc ttatccccct
    12901 taaacgcaag gtacgcattg cgccccagcg catacaggat attctgcgaa tactgcttga
    12961 acaggaagac caccttcgcc gcattgccca tcataaagcg cggacggttt tgcgccgcat
    13021 agtcaaaatg cccgtcgtac gtcgcctttt tcgcctgttc gaaagccgcc tcactgtccg
    13081 cccccgcccg tttcgccaaa cggtaggccg cgacaaacgt aacctggcgg ttaaacttct
    13141 ccgcatgatg gaacagccag gccgccttat ccatcacctt ctgccaagcc gaccccgcca
    13201 accccgggtc gccgttggcc acccccgcca aatcatgcgc ctgcgacaaa tcgatcacac
    13261 cgtaatccgc cgcctttcgg aacgccgcct tctcatcccc gttcaaactg tcctcaatcg
    13321 tattgaactt ctccccgaac ctcagcccga tctgttttga agcccgcagc aattcccgcg
    13381 ccgcaccggc ataaccccac ttcgccgcca tcaccggata agccaccatt gccgtctgcg
    13441 acaggttcac aaccgcagaa gcgggcgaca tccccatcat ccacaaaaag ccgaagcccg
    13501 tcaaagcctg cgccagcttg gaaggattcg gattcatcac cgcctcgtgg cgtttcctca
    13561 tctcatccgc cacacgctgc aactgacgct ggtcgaaacc ctcctcatat ttgcgcccgt
    13621 ccacaaaatc ctgcatcaca tccaactgtt ccgccatacg gtccgcatag cgcaacttcg
    13681 ccagataatt cgcaccgctg cccatattct gcgcatatgc gcgcctcgca tcatcgctga
    13741 agcccggcac gcccttgcgg tggatgccgt gtttcgccca agacgtatcg ggcagggagt
    13801 tcaaataaag ctgcgtcagc gtatcgttca atcgggcccg ttgcgccgga tccaaatcca
    13861 tcctgccgac agcctcgccc agctccttca taaatccgct gccgaccgca tcgcggcttc
    13921 ggatataatc ccgggacttc atcacgggcg aaaccttaaa ccccggcccg aaatcagcct
    13981 tcagcgcatc gcgcagcttc tccgcctcgc tcaaagtttc cgcgcgggac acattcgcac
    14041 tattcccgtc cgcatccttg accaccacca gataatcgcc gaaacgcgcc aaagggaaat
    14101 acacaccgcc cagctcgttg tcaaaacgct ccttcaggcg gcgcaccgtt tccgcatcct
    14161 gccccgcacg cgccaaccgt tcggcaagcg catcgcgcac ctgcccgaaa tgcgccctat
    14221 aatcatcccg cgccttgcgg tacaccctct gcgccgcatc aggcagcccg gcaaaacggc
    14281 ggttcagcaa agcaagcccc tccaaagcct tttgcgcccc aacctcatcc ttttccaaac
    14341 gcttcacatc cgcgcgcgcc tgctccaaca tccgtaccgc atccgtttta gcccggcttt
    14401 ccgccgccgc atccgccccg gcgcgcctca acgcccgctt cgcctccgaa tccgcataaa
    14461 acagacggcg caggcgcata tccgcctcat ccgccaaaat ttcccgaccg tgtttttcct
    14521 gcgccttctc cagcgcataa gccgccttat ccgccgcccg ttgcgcctta ttgtaagcag
    14581 catccgcacg cgcggtacgc gcaccggcgg aagcaacggc cgcccgcgcc ttcgcgattt
    14641 taccgtccgc aatatccaaa gccgtccgga tgccgtccaa acgcccccgg gcatcctttc
    14701 gcatcagcgg gtcggcatcc accttcgcca gcgtcgcatc gtgcatcaga tccgcaagcg
    14761 ctttcgcatc ctcctccttc aacctgcccc attccctgac aaccttgtcc gcctccgtaa
    14821 ccgccttgtt cgcatccgcg ccgaagagtt ccgacaaacg cccgtattcc ttcaggccgt
    14881 ccaaaacgcc gccgtaaatg tcctcgatct gccgcctgcc caaaaactgg agctgaatcc
    14941 ccttggaagc atcccagcgg tctttcagcg cgtttctcaa atcggatttt ttacccgtta
    15001 tacggctgat tgcggccccg atgttcgccg cacgggaaaa acgcaccgcc tccgtgtcaa
    15061 aatcggcaat gccgcccttg cccgcatctt tgtcttgcaa aatctgcaac aggcgtttat
    15121 catcaatgcc cgataaaggc gatttgcttt cagaggctcc cgtagttgat acgcggtcaa
    15181 gtaatgaaag caagttgcct ttttctattt tcgtcagctt atggtcgtaa tagcggttac
    15241 cgttcctgct aaccgcgacg gcagccctta ccgtgtaatc cgcaccgccg acattcagtc
    15301 ccgaaacata atattcataa ccctgaatat cgccgttctt agccaaatcc tcgttcggca
    15361 gcgtatcgat atacaccgca ttctcaataa tctgcggaat tgccgcgata ctctgcaaat
    15421 gttccgcgtc cttatagtcg tgacgcaata tttcagtgat gcccgaacgt cccaaactga
    15481 tttcacgccc cgtgtcctta ttcacataag gcccgcgcaa agatttgccg tattccagcg
    15541 cattgcgctt atattcgcgc aaattgccgc cttccggaac ctccctgccc gagatacgga
    15601 tggtttccgc acggcgcagc ttctcaaggc tttccgactt ggaacgttcc gccgcaccgt
    15661 cttcagcccg cgcgaacatc actgcacgat gattgccgtc ccaaggcgca tcccgcgccc
    15721 cttccacatt cccgtgcaaa tccgccagca tcgcaaacac atccgcgtcc gacatcgcac
    15781 cgccggcctt gcccgccagg cgttgcagca cacggcgcaa cagggcgccg atacgcgcca
    15841 acacgcccgc aatgccgcgt ttcaaaccgt tgccgacctt cacgccgtaa cggttttcaa
    15901 tgcccgccca accgccggta cgctgcgccg cataaagctc cgcgaccgcc tcttccaccg
    15961 cggcggagcg caccgaggcc gccgcatcgc ccgtgccttc gcgcccttcc tgcaccgcgt
    16021 ccgcaatgcg ccgtatcagg ccgttgccgt ccgcacgttc caattcttca cggtacttgg
    16081 cgaaaccatc cgccgcaaag cccctgtgcc ccagctcgtg ccacgccgcc cataccgcgc
    16141 gttcgggcgt aaggttttcc gccaccagcg tgattttgcc cgtcctgccg tcaaaccagc
    16201 cctccacacg ctgcgacaac agcatctgcg ccttgtccgg cgccgtttcg gacacggcgg
    16261 cgacatccac ccgttccgcc agtccgccta tgctttcggc gaggcgtttt ttgatatttt
    16321 cggcgtgctc gcgcaatgcc ttaaaacctt gcggcgcctg acgctctttc accgtataaa
    16381 caggccctcc ctgcaacaca cgcgccttgc gccgtgcctc ttccggattg tccgaagccg
    16441 ttttcgcaaa cgtttcaggc ttggcagcct tatccgaaga cggcttttcg gttgccgcgc
    16501 cttgtgcggc ggccgacttg ccctttccgc catcgaagtg ggattttccc gattcgatat
    16561 ttcgcacctt atccgaagac ggcttttcgg ttgccgcatt ttttgcagca gccgccttgc
    16621 ccgcaacagg cgtatctttt gcccgggcag gctgcgccct gcccgccggt ttgccgtcgg
    16681 cggcggcatc gcgcggccgc gccatcaccc tggccggcgc ccttgcttcc tgcgtcggac
    16741 gataatcccg gcccggctcg gacggcacgg cttccgacaa accgcccgcc cgcaaggttt
    16801 ccgcctcccg ccccttcaaa taacggcggc cgtcggagac aaggccgtca gagttggaag
    16861 gaccggccat atcggcaaac tcctccggca tcccgccttt gcccggcctg cccggcacgc
    16921 ccgcaagcgc atcaatatgc ctgcggtccg tatgctcgaa acggtattcg ccgcccccca
    16981 aaggcacacc cgccttttcc ggcctgcgcc tgtccgccgc cgcatccgcc atccgtttct
    17041 taggcgatac cctgcccgcc gaagccgcaa cctgcggatt caataccggc aacaccgtat
    17101 cggggcgttt gcccgcaaca ggcgcacgtt tcccgccgtc cgtcccggca aggttttcag
    17161 acgcggacag gccgggcgcg gcaccttccg cgcgcgcccg aacctgtaag ttttgccgac
    17221 gggccggcgc attgccggac gcaacggcgg accctggcaa accctgcaag gcagccgcat
    17281 ttgcatccgc ccccgcatca ggggtcaaac ccctgccgcc ttgggtcaaa ccctgccgca
    17341 catcctgccg cacatcctgc gccaaaccct tatcttccgt gggtaaagcc cgggtcaaac
    17401 ccctgccgcc gccgacaacg gcctcagacg cgccgtcatc cggcaacgcc gtccgaaccc
    17461 cctgcaaacg ctccccctcg cggctcaaca gcggcacgct ccaaccgttg ttgcggcggt
    17521 aaccgtaaac cgcacggtta agcctgtcca acacatccgc cgattccgga tgttgcgcac
    17581 ggtaagcgcg tacagccccg gccgattccg ccaaaccctc aggataatgt ccgcgcatat
    17641 ccgccatacc cgcctcccgg cgcataatcc cgtccgccgt atagtccgcc accgtctgcg
    17701 catgacggcg cacccattca ggcagcgcgt caaaccgcct gcgcgattcc ttatccaacg
    17761 catcggcagg gacggaaaac ccttttttag gaacaggcac ggcatccgcc atacgcacgg
    17821 gcggcggcag gtaaccctgt ttggcttcgg aagccgcctg ataatccgcc tcaatccgcg
    17881 cgtgatacgc ctgctcgatt tttcggcgca caggcgcggc gatttgcccg aaatccgcaa
    17941 cgcccttaat atcaaggccc gcctccttcg ccaaagcctt gcgcccccgg gtatccaagc
    18001 cgtcgaaata ttggcccgca ggcagcggcg agacccgcgc aatcccgccc gaagcggcgg
    18061 cggcagaacc gcccggcgca accgaagccg caccgcgcgg cgcaccgccg gggcgcacat
    18121 tgccgtaagc ggacggaaca ccgcccctgt ccgcatccgc cgaccgtccg gacggcgcat
    18181 catccgcaac cgaccgggct tccatatcgt cagacaccgc agacggcaca gccgcgcccg
    18241 aatccgccgc caaagcagcg gccgcagaaa ccgcaccgtc atccggatca atccccaccg
    18301 cctccgacgg cttggcccgg tatttagccg ccatgtccgc atactgacgc gccaaaccgt
    18361 ccggcatcac gcccgtatcc aaaaattccg ccccgtcctc cgacgggata cccgaaacat
    18421 ccggctgttt ttccgtcccg cgcgccaatt cctccgcgtg cggcgtattg ccgtcggcag
    18481 ggccgctgaa aaaccgcccc gcctcctcct gcttcgtacg gtccgcaaac tgccgcaaag
    18541 ccggatgcga ctgatcgtga tgcgccgtat caaaacccgc gccgccctgt tccgcgccgc
    18601 cgtccgcagc cgcctgtgcc gccgcacgca acgcctgcgc cccttccggg gccggcct
//
LOCUS       DAWTQS010000040        18519 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.040, whole genome shotgun sequence.
ACCESSION   DAWTQS010000040 DAWTQS010000000
VERSION     DAWTQS010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18519
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.040"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(133..1554)
                     /locus_tag="WNA25_001346"
     CDS             complement(133..1554)
                     /locus_tag="WNA25_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HFA9824088.1"
                     /translation="MRHLPLFSLMLFPASVYAADSDGANLNLLWSLPFALILLSIALG
                     PLFFSHTWHHHYGKITAFWTLLFLIPFSLVFGASAGIHTVAHALVEEYIPFILLLLAL
                     YTISGGILVWGDLNGTPKLNTALLAVGTALASIMGTTGAAMLMIRPLLKANQDRTRRV
                     HIVIFFIFLVANIGGGLTPLGDPPLFLGFLKGVDFMWTVKHMFAPVLISTAVLLTAFY
                     FIDNRFFKQESIAQDTPAQQEKPEKIAIFGKWNFLLLSGVVGAVLMSGLWKPEHPGFE
                     ILGSRYALQNLVRDVILITLTAVSMAITPKQVRAGNEFNFEPIAEVGKLFLGIFITIF
                     PVLSILKAGEAGALGGVVSLVHDTAGHPINTMYFWMSGILSAFLDNAPTYLVFFNMAG
                     GDAQALMTGPLFHSLLAVSMGSVFMGALTYIGNAPNFMVKAIAEQRGVPMPTFFGYMM
                     WSVAFLTPVFIVHTLVFFVFKLL"
     gene            complement(1569..1859)
                     /locus_tag="WNA25_001347"
     CDS             complement(1569..1859)
                     /locus_tag="WNA25_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HFA9824089.1"
                     /translation="MQIYVKINSFFVFDRLFIKDIYLFVLNKIIYQKEHIFPSTAIRL
                     PAAFRFGKLVYPDYLLYNQSENFFQKQTGSTVRLCPGGTVPVSLPRAAQGSA"
     gene            complement(1912..2271)
                     /locus_tag="WNA25_001348"
     CDS             complement(1912..2271)
                     /locus_tag="WNA25_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HFA9824090.1"
                     /translation="MIRYLLIACGCISLLLGIIGIFLPLLPTTPFVLLSAACWAKASP
                     RFHRWLHRHRYFGPMVHNWEQNGAVPRKAKIFAISMMTASCLIMFWQFPQRWWVGAVS
                     SVFCSLVAIWMWRRPES"
     gene            complement(2268..2873)
                     /locus_tag="WNA25_001349"
     CDS             complement(2268..2873)
                     /locus_tag="WNA25_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HFA9824091.1"
                     /translation="MTIMNINTSENKDAVAEHTGQWLEKAVIGLNLCPFAKAPHVKNL
                     VRIAISEAKHLDSFLEDLDEELQRLGNTPATELETTLLVHPTLFPDFDVFNDMLDIAD
                     AAVIENGLEGIVQIAPFHPQFRFQNTEPDDIGNYTNRSPYPTLHLIREDSIAKAAQAF
                     PDASAIFERNIALLEKMGHEGWAKLGITSCPYPHNKKNISK"
     gene            complement(2920..3402)
                     /locus_tag="WNA25_001350"
     CDS             complement(2920..3402)
                     /locus_tag="WNA25_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HFA9824092.1"
                     /translation="MPQLTLDKTDIKILQVLQENGRLTNVELSERVALSPSPCLRRLK
                     QLEDAGIVRQYAALLSPESVNLGLQAFIRVSIRKAKDAREDFAASVRKWPEVLSCFAL
                     TGETDYLLQAFFTDMNAFSHFVLDTLLSHHGVQDAQSSFVLKEIKHTTSLPLNHLLKE
                     "
     regulatory      3606..3703
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            3913..5013
                     /gene="gcvT"
                     /locus_tag="WNA25_001351"
     CDS             3913..5013
                     /gene="gcvT"
                     /locus_tag="WNA25_001351"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFA9824093.1"
                     /translation="MITLKTTPFHQAHQDAGAKPVDFAGWELPIHYGSQIAEHEAVRT
                     DAGMFDVSHMLVTDVAGANAKAFFRKLIANDVAKLAFVGKALYSALLNDNGGVIDDLI
                     VYRTNEAETQYRIVSNGATREKDTAQFHKVGQEFGVAFNPRYDLGMLAVQGPKAIEKL
                     LTVKPEWADVIHGLKPFQGADLGNDWFVARTGYTGEDGVEVILPGTEAVAFFKALQAA
                     GVQPCGLGARDTLRMEAGMNLYGNDMDDDTSPLEAGMGWTVDLKDESRDFVGKAALLA
                     LKEKGVAVKQVGLLLGKGGILRAHMEVLTDKGKGETTSGVFSPSLKQSIAIARVPKDF
                     DGDTAKVLMRGKEVDVRVLKLPFVRSGQKQFD"
     gene            5160..5699
                     /locus_tag="WNA25_001352"
     CDS             5160..5699
                     /locus_tag="WNA25_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824094.1"
                     /translation="MFFDTEGKSHTAIFMKIVGGNGKIAGEVVIDGEYALDIVRMNDD
                     LSIAVQDNKGRSRASDLEALSQAKSFTLIAFSRYAGTVEVDKVSAVTGICADYRSKGL
                     KVAVADNMRPKQEIVVFRASGVMASNKRKINEAYLDYSASSKLPSAQKQELEQDKNNM
                     IAVHYGRLEELIARGICVR"
     gene            5751..6137
                     /gene="gcvH"
                     /locus_tag="WNA25_001353"
     CDS             5751..6137
                     /gene="gcvH"
                     /locus_tag="WNA25_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFA9824095.1"
                     /translation="MSNNIPTELKYVASHEWLRLEEDGTITVGITHHAQELLGDIVFV
                     ELPEVGANLAAEEQSGVVESVKAASDVYAPIAGEVVAVNDDLPGAPETANSDPYGAGW
                     FFKIKPANPADYDGLLTAEQYAGEVD"
     gene            6290..7537
                     /locus_tag="WNA25_001354"
     CDS             6290..7537
                     /locus_tag="WNA25_001354"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFA9824096.1"
                     /translation="MQLTAVGLNHQTAPLSIREKLAFAAAALPEAVRNLARSNAATEA
                     VILSTCNRTELYCVGDSEEIIRWLADYHSLPIEEIRPYLYTLDMQETVRHAFRVACGL
                     DSMVLGEPQILGQIKDAVRAAQEQESMGAKLNALFQKTFSVAKEVRTDTAVGENSVSM
                     ASASVKLAEQIFPDIGDLNVLFIGAGEMIELVATYFAAKNPRLMTVANRTLARAQELC
                     DKLGVNAEPCLLSDLPAILHDYDVVVSSTASQLPIVGKGMVERALKQRQSMPLFMLDL
                     AVPRDIEAEVGDLNDAYLYTVDDMVNIVQSGKEARQKAAAAAETLVSEKVAEFVRQQQ
                     GRQSVPLIKALRDEGEKARKQVLENAMKQLAKGATAEEVLERLSVQLTNKLLHSPTQT
                     LNKAGEEDKDLVHAVAQIYHLDK"
     gene            7709..8899
                     /locus_tag="WNA25_001355"
     CDS             7709..8899
                     /locus_tag="WNA25_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HFA9824097.1"
                     /translation="MIHIISILKSIGISGIAMSCFSIRRMSAFRARITAFFTAFVFLT
                     AALPAYAERLPDFLAKIQPSEIFPGADRYGKPEGKPMVARVYKGDEQLGLVYITTDAV
                     NTRGYSSKPIDTLMALANDGTIAGAKLVDHHEPIMLIGIPQSRVDKFIDKYIGLNFIK
                     NPPTPGVAPGDIISGATVTLMVVNDSIQRSYKVIANQYRLGSDKALQTASASDVREAA
                     PASETRPRRMANPDKQDILSWDELLKQKAVGHLHITLDQINKLFEKGGKAGVADHAEQ
                     GDPDDTFIDLYVALVSQPSIGKSLLGKDGWAHLQKRLKPGQQAVLVAGEGRYSWKGSG
                     YVRGGIFDRIEMIQGENSFRFTDAQHERVVELSAADAPRFKEVSWFTIPEGVAFDGAE
                     PWRL"
     gene            8903..10090
                     /locus_tag="WNA25_001356"
     CDS             8903..10090
                     /locus_tag="WNA25_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFA9824098.1"
                     /translation="MVQRVLSVNDKAFVTADLDYELPQAYYVDDPKAPPVEISAPVEA
                     VPAAASDTASDGIAEDASAENGVSNQLWKQIWKAKQGQIVVVGIALTILLLVFLFQDW
                     IVRYEKWYDRFRFAFLTFTLFYIGWYAQAQLSVVNTLTLFSAILTEFHWEFFLMDPIV
                     FILWLFTAATMLLWNRGTFCGWLCPFGSLQELTNRIAKKLGVKQITVPHMLHTRLNVI
                     KYLIFFGFFAISLYDLGTAEKFAEVEPFKTAIILKFMCDWWFVAFAVALLIAGLFIER
                     FFCRYLCPLGAGIALPGRFRVFDWLRRYKMCGNPCQICTHECPVQAIAPEGDIHPNEC
                     IQCLHCQVMYHHDTRCPQVVAENKKKQKQAAAKSGELENVSKQPQEQVVRFVKPETAQ
                     SEK"
     gene            10219..12186
                     /gene="nosZ"
                     /locus_tag="WNA25_001357"
                     /pseudo
     CDS             10219..12186
                     /gene="nosZ"
                     /locus_tag="WNA25_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712466.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-dependent nitrous-oxide reductase"
     gene            12359..13714
                     /locus_tag="WNA25_001358"
                     /pseudo
     CDS             12359..13714
                     /locus_tag="WNA25_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712464.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
     gene            13772..14668
                     /locus_tag="WNA25_001359"
     CDS             13772..14668
                     /locus_tag="WNA25_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFA9824099.1"
                     /translation="MTTHHVELRKVTKRFGAQKAVSQVDLVLKAGESVGLAGHNGAGK
                     STIMKLILGLITPTEGEVMLLGERTGSKAGARLRSQIGYLPETVALHPSLTGIETLDF
                     YAKLKKQPLTQNRGLLERVGISQTAHRRVGTYSKGMRQRLALAQALLGEPKVLLFDEP
                     TTGPDPASRQMFYEVVRELNGRGATVLLSTHALAELDGHADRIVVMKNGVKVADGSMD
                     ELHVQSGLPLTVNIRLNAPRTLSSRWQPLSDGISYRAQCQAEERMELLGELGSLSDLA
                     YFDIHTPTLDEMYARFLKREDV"
     gene            14665..15495
                     /locus_tag="WNA25_001360"
     CDS             14665..15495
                     /locus_tag="WNA25_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFA9824100.1"
                     /translation="MNPVWIITGKEARDSLRNRWVLAAVLLLAALALSLGFLGSSPTG
                     SVKVDPLTVTVVSLSSLSIFLIPLIAMLLSYDALIGEIERGTMALLLSYPIWRNQILA
                     GKFVGHLIILALATTAGYGLAGITLQLANGGFDIAVWKPFALLIAASVILGAAFLSMG
                     YLISAKVKERGTAAGISIGVWLFFVVIFDMALLGILVADSKQVITAPVVETVLLFNPT
                     DIYRLLNLTGYENTAMYAGMAGLSGQIGLTVPVLLTAQVLWVIIPLVLAAGIFRKRRI
                     "
     gene            15492..15986
                     /locus_tag="WNA25_001361"
     CDS             15492..15986
                     /locus_tag="WNA25_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HFA9824101.1"
                     /translation="MKKTLLAIVAVFALSACRQAEEAPPPLPRQISDRSVGHYCSMNL
                     TEHNGPKAQIFLNGKPDQPVWFSTVKQMFGYTKLPEEPKGIRVIYVTDMGNVTDWTNP
                     NADTEWIDAKKAFYVIDSGFIGGMGAEDALPFGNKEQAEKFAKDKGGKVVGFDDMPDA
                     YIFK"
     gene            complement(16120..17781)
                     /locus_tag="WNA25_001362"
     CDS             complement(16120..17781)
                     /locus_tag="WNA25_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HFA9824102.1"
                     /translation="MTESITRDSMQYDVVIVGAGPSGLSAAIKLKQLAEQNGREISVC
                     VAEKGSEAGAHSLAGAVIDPIALNELIPDWKEKGAPLTRTVTQDKVLFLTEKKAFNLP
                     ITPNFDNHGNYIVSLGEVVRWLAEQAENMGVEIYPGFAAAEVLYHEDGSVKGIATGNM
                     GVGKDGGPTDSFQPGMELWARQTLFAEGCRGSLSKQIIERFQLDQNSQPQTYGLGIKE
                     VWEVPSEQHQPGLVVHSAGWPLDSKTYGGAFIYHFDDNKVAVGFVVGLDYQNPYLSPF
                     EEFQRFKTHPEIRKTFEGGRRIAYGARSLIEGGLQSLPKLSFKGGVLIGDAAGFLNMP
                     RIKGIHTAMKSAMLAAEAVFPLLENFEEVESFDSGKEAGNYQKLFEQSWLYQELYAAR
                     NVRPSFKWGVYLGSLYTGIDQMIFRGKAPWTLKHHGKDNEQLKKAAVCKPIDYPKPDG
                     VLTFDRLSSVFLANLAHEENQPDHLVLNNPQTMIDVNYKEYASPETRYCPAGVYEIIE
                     ENGSPRLQINAANCVHCKTCDIKDPTQNITWICPEGASGPNYGGM"
     gene            complement(17911..18453)
                     /locus_tag="WNA25_001363"
     CDS             complement(17911..18453)
                     /locus_tag="WNA25_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFA9824103.1"
                     /translation="MQSWKARRDFNIVKQDLDFSCGAASVATLLNNFYGQKLTEEEVL
                     EKLGKEQMRASFEDMRRIMPDLGFEAKGYALSFEQLAQLKIPVIVYLKYRKDDHFSVL
                     RGIGGNTVLLADPSPGHVSMSRAQFLEAWQTREGNLAGKILAVVPKGRDAGGDKAFFT
                     RSPKRQTEFAVGQVKWWRAY"
BASE COUNT         4489 a         4797 c         4982 g         4251 t
ORIGIN      
        1 ttaacttcgg cacaccgtcc cggcagctaa aaatcctgcg ggatcggtgt ggaatttagg
       61 gataatctag tacagcccct ttgattatgc cggaatttaa aatgccgtct gaatgttcag
      121 acggcatagc gtttacagta gtttgaaaac gaaaaagacg agggtatgta cgatgaagac
      181 gggtgtcagg aaggcgaccg accacatcat atagccgaag aaagtcggca tcggtacgcc
      241 gcgctgttcg gcaatggcct tgaccatgaa gttcggtgcg ttgccgatgt aggtcagtgc
      301 gcccatgaat accgaaccca tagaaaccgc cagcagcgaa tgaaacaggg gacccgtcat
      361 taaggcttgg gcatcgccgc ccgccatatt gaaaaacacg agataagtgg gcgcgttatc
      421 caagaatgcc gacaatatgc cgctcatcca gaaatacatc gtattaatcg gatgacctgc
      481 cgtatcgtga accagcgata ccaccccgcc cagcgcgcct gcctcgcctg ctttcagaat
      541 gctcaggacg gggaagatgg tgatgaagat gccgaggaag agtttgccca cttcggcgat
      601 gggttcaaag ttgaattcgt tgcctgcgcg gacttgtttg ggcgtgattg ccatagatac
      661 ggcggtcaat gtaatcagga tgacatcgcg gacaaggttt tgcagggcgt aacggctgcc
      721 gaggatttca aatcccgggt gttcgggttt ccaaaggccg gacattagaa ccgcgccgac
      781 cacgcccgaa agcaggagga agttccattt gccgaagatg gcgatttttt cgggtttttc
      841 ctgttgtgcc ggcgtatctt gtgcaatgct ttcctgtttg aagaaacggt tgtcgatgaa
      901 atagaaggcg gtcaacagga cagcggtgct aatcaggacg ggggcgaaca tatgtttgac
      961 cgtccacatg aaatctacgc ctttgaggaa gccgaggaag agtggggggt cgcccaaagg
     1021 ggtcaggccg ccgccgatgt ttgcaaccag gaaaatgaag aagatgacga tgtgcacgcg
     1081 gcgggtacgg tcttggttgg ctttcagcag cggacgaatc atcagcattg ccgcgccggt
     1141 cgttcccatg atagaggcaa gtgccgtacc gacggcaagc agggcggtgt tgagcttggg
     1201 tgtgccgttc aagtcgcccc aaaccaaaat gccgcctgaa atggtgtaca gggcaagcag
     1261 cagcaggatg aaggggatat attcttcaac gagtgcgtgt gcgacggtat ggataccggc
     1321 ggacgcgcca aaaaccaaac tgaacgggat gaggaagagc aatgtccaaa aggcggtgat
     1381 tttgccgtaa tggtgatgcc aggtatgcga aaaaaacaag ggacccaatg cgatagacag
     1441 caaaatcagg gcaaagggca ggctccacag caggtttagg tttgcgccgt ccgaatctgc
     1501 ggcgtaaacc gatgcgggga aaagcattag tgaaaacagg ggtaggtggc gcatcgtgtt
     1561 tcctcgattc aagcactgcc ttgcgcggcg cgtgggagtg atacaggcac cgtgccgccc
     1621 ggacataggc ggactgtgct gcctgtttgt ttttgaaaaa agttttccga ttgattgtaa
     1681 agtaaataat caggataaac cagtttccca aaccggaagg cggcgggaag gcggattgct
     1741 gtgcttggga atatatgttc tttttgataa ataattttat ttaaaacaaa taaatatatg
     1801 tctttaataa ataatctgtc gaaaacgaaa aatgaattta ttttaacata tatttgcaat
     1861 gaaacaggtt tgccccccgt ttgtttgccc ttatcccttt cagtacggca ttcaagattc
     1921 gggcctgcgc cacatccata tggcgacaag ggaacaaaaa accgatgaaa ccgccccgac
     1981 ccaccagcgt tggggaaact gccaaaacat tatcaggcag gatgcggtca tcatactgat
     2041 ggcgaatatt ttggctttgc gcggcactgc gccgttttgt tcccagttat gaaccatcgg
     2101 accgaaatag cggtgccggt gcagccagcg gtgaaagcgc ggggatgcct ttgcccagca
     2161 ggcggcggag agcagtacga acggcgtggt cggcaacagc ggcaaaaaaa tgccgatgat
     2221 acccaacagc agggaaatgc agccgcaggc aattaaaaga taacgtatca ttttgaaata
     2281 tttttcttat tgtgcggata agggcaggat gtgataccga gttttgccca gccttcatgt
     2341 cccatttttt ccagcagggc gatattgcgt tcgaatattg ccgaagcgtc gggaaaggct
     2401 tgtgcggctt tggcaatgct gtcttcgcgg atgaggtgca gcgtcggata gggagaacgg
     2461 ttggtgtagt tgccgatgtc gtcgggttcg gtattctgaa agcggaattg gggatggaac
     2521 ggtgcgattt ggacgatgcc ttccaagccg ttttcgataa cggcggcatc ggcaatgtcg
     2581 agcatatcgt tgaatacgtc gaaatcgggg aatagggtcg ggtgaaccag cagggtggtt
     2641 tccagttcgg tggcgggtgt attgcccagt cgctgcagtt cttcgtccaa gtcttccaaa
     2701 aaactgtcaa ggtgtttggc ttcgctgatt gcgatgcgga caaggttttt aacgtggggg
     2761 gctttggcaa agggacacag gttcagaccg atgacggctt tttccaacca ttgtccggtg
     2821 tgttcggcaa cagcatcttt attttcggaa gtattgatat tcattattgt catgtaaatg
     2881 tgtttgcaga ttgcacgtgc gggaaaatcg ggaagggcac tattccttca gcaggtggtt
     2941 gagcggcagg gaggtggtgt gtttgatttc ttttaaaaca aagctcgatt gcgcatcttg
     3001 tacgccgtgg tgggacagga gcgtatccaa aacaaaatgg gaaaacgcgt tcatatcggt
     3061 aaaaaacgcc tgaagcaggt agtcggtttc ccctgtcagg gcgaagcagc tcaagacttc
     3121 cggccatttt cggaccgatg cggcaaagtc ttcccgcgcg tcttttgctt tgcggatgga
     3181 aacgcggata aatgcctgaa gccccaggtt gacggattcc ggagacagca gcgcggcata
     3241 ttggcggacg atgccggcat cttccaactg cttcagacgg cgcaggcacg gagaaggcga
     3301 aagtgcgaca cgttcggaca gttcgacatt ggtcagcctg ccgttttcct ggagaacctg
     3361 taagatttta atatcggttt tgtctaaagt gagttggggc atatttgcgt tccgttttaa
     3421 ggaattcgga ttgtctgtcc gtatgtttgc ggcaatccgc acagatggag accatattaa
     3481 catataaaaa gttataccgt catccgggac aaattttgtt ttcggaaaat catgtgaaaa
     3541 cagaggcggt cggtttgcat ctcttttaag acggcttgcc caaaccgccg attcaagaca
     3601 taatcgggaa atgtgcagga gagtgttaca cccaactaca atgtaaccac cgaaggcgca
     3661 gacaccctta aatcgctcag gtatcaggga ctgcacattg aaacaaacaa tctggagagc
     3721 ggcgttggaa taacgtccac cgaaggggag aaggccgtct gaaccaccat tcagacaacc
     3781 gcgcaaagca gtgagcagac tggtttgcca tcatgcggat acggccgaaa atctcaggtt
     3841 caaggacaga tagggtcatc cgcgcatttt gtgcggacgg catctgaaca aaaaatccgg
     3901 agaaacttga gaatgattac tctgaaaacc accccatttc atcaagccca tcaagatgca
     3961 ggcgcgaagc cggtagattt tgccggctgg gagctgccca tccattatgg ttcgcaaatc
     4021 gccgaacacg aagccgtgcg caccgacgcc ggtatgtttg acgtatccca tatgcttgtt
     4081 accgacgtag caggcgcaaa tgccaaagcc tttttccgca aattgattgc caacgatgtc
     4141 gccaagctcg cttttgtcgg caaagccctt tattccgctt tgctcaacga caacggcggc
     4201 gtgattgacg acttaatcgt ttaccgcacc aacgaagccg aaacccaata ccgcatcgtg
     4261 tccaacggag cgactcgcga aaaagacacg gcgcaattcc acaaagtcgg acaagagttc
     4321 ggcgttgcct ttaatccgcg ctacgacctc ggcatgctcg ccgtacaagg ccctaaagcc
     4381 atcgaaaaac tcctgaccgt caaacctgaa tgggcggatg tgattcacgg cctcaaaccg
     4441 ttccaaggtg cagacttggg caacgattgg tttgtcgccc gcaccggcta taccggcgaa
     4501 gacggcgtcg aagtgattct gccgggcact gaagccgtcg cattcttcaa agccctgcaa
     4561 gcagcaggcg tacaaccttg cggtctcggc gcgcgcgaca ccctgcgcat ggaagccggc
     4621 atgaacctct acggcaacga tatggacgac gataccagcc cgctcgaagc aggcatgggt
     4681 tggaccgttg atttgaaaga cgaaagccgc gattttgtcg gcaaagctgc cttgttggca
     4741 ttgaaagaaa aaggcgttgc cgtcaaacag gtcggtttgc tgctcggcaa aggcggcatc
     4801 ctgcgcgcgc atatggaagt gttgaccgac aaaggcaaag gcgaaaccac cagcggcgta
     4861 ttctcgccca gcctgaaaca atccatcgcc atcgcccgcg taccgaaaga ctttgacggc
     4921 gataccgcta aagtgctgat gcgtggcaag gaagtggacg tgcgcgtact gaagctgccg
     4981 ttcgtccgca gcggtcaaaa acagtttgat taatatcggt tcagacggca tttgtttgaa
     5041 ttaagccgtc tgaaagcagg ttttaaggaa ttgttatttc ctatgaaagc aaattttggc
     5101 agtttattgt tgttattgat gttatcggct tgcgcttcgt cgccggcgag cgcggattga
     5161 tgtttttcga tacagaagga aaatcccata ctgcaatatt tatgaaaatc gttggcggca
     5221 atggaaagat tgccggcgag gtggtgatag acggggaata tgctcttgat attgtccgca
     5281 tgaatgatga tttgagtatt gccgtgcaag ataataaggg tagaagccgt gcttccgacc
     5341 tggaagcatt gtcgcaagcg aaatcattta ccctaattgc gttcagccgt tatgcgggca
     5401 cagttgaagt ggataaagta tcggctgtta caggcatttg tgcagattac cgcagcaaag
     5461 gcctgaaggt tgccgttgca gacaatatga ggcccaaaca agaaatcgtt gtattccgtg
     5521 caagcggcgt aatggcaagt aataagcgca agataaacga agcatatttg gattattctg
     5581 catcctctaa actgccttcc gcccaaaagc aagaattgga acaagataaa aacaatatga
     5641 ttgccgtcca ttacggacgc ttggaagaat taatcgcacg aggtatctgc gtacgctaag
     5701 ttgtgcagat gccgtctgaa aataaccccc taccaatgga gaatcaaacc atgagcaaca
     5761 acatcccaac agaactgaaa tacgttgcca gccatgaatg gctgcgcctt gaagaagacg
     5821 gtaccattac cgtcggtatt acccatcacg cgcaagagct gttgggcgac atcgtgtttg
     5881 tcgagctgcc cgaagtcggt gcgaacctcg ccgccgaaga acagtccggt gtggttgagt
     5941 ctgtaaaagc cgcatccgac gtgtatgcgc cgattgcggg cgaagtcgtt gccgtcaacg
     6001 atgacttgcc aggcgcgccg gaaaccgcca acagcgaccc ttacggcgca ggctggttct
     6061 tcaaaatcaa acctgccaat cctgccgatt acgacggtct gctgactgcc gaacaatacg
     6121 cgggcgaagt ggattaaacc gcccgtttgt ccgacggcaa ccgtcggaca aacggaaact
     6181 gcaccttcag acggcatttc tgcggtcgga ggtgcagttt ttttgtccgt gttttaagga
     6241 tgcagttagg ctataataac ggtctatatc catcttcacc gattttttca tgcaacttac
     6301 cgctgtcgga ctcaatcatc aaaccgcacc tttaagcata cgggaaaagc tggcgtttgc
     6361 cgccgccgcc ctgccagaag ccgtccgcaa tcttgcccga agcaatgcgg caacggaggc
     6421 ggtaatcctt tctacctgca accgcaccga gctttactgc gtcggcgatt cggaagaaat
     6481 catccgatgg cttgccgatt accacagttt gccgattgaa gaaatccgtc cgtatctgta
     6541 cacgctggat atgcaggaaa ccgtgcgcca cgccttccgc gttgcctgcg gcttggattc
     6601 gatggttttg ggcgagccgc agattttggg gcagattaaa gatgcggtgc gtgcggctca
     6661 agaacaggaa agtatggggg caaaactcaa tgccctgttc caaaaaacct tttccgttgc
     6721 taaagaagtc cgtaccgata ccgctgtcgg cgaaaattcg gtttcgatgg cttccgcgtc
     6781 cgtcaagttg gcggaacaga tttttcccga catcggcgat ttgaacgtat tgtttatcgg
     6841 cgcaggcgaa atgattgagc tggttgccac ttattttgcc gccaaaaatc cccggctgat
     6901 gacggttgcc aaccggacgc tggcgcgtgc acaggagttg tgcgacaagc tcggtgttaa
     6961 cgcggaaccg tgcctgctgt ccgatctgcc tgccattctg cacgattacg acgtggtggt
     7021 ttcttcaacg gcgagccagc ttccgatagt cggcaaaggc atggtcgaac gcgcattgaa
     7081 acagcgtcag agtatgccgt tgttcatgct tgacttggcc gtgccgcgcg atattgaagc
     7141 ggaagtcggc gatttgaacg atgcgtatct ttatacggtg gacgatatgg tcaacatcgt
     7201 ccaaagcggc aaggaggcaa ggcagaaggc cgccgccgcc gccgaaacgc tggtgtccga
     7261 aaaggttgcc gaatttgtca ggcagcagca gggcaggcag agcgttccgc tgattaaggc
     7321 cttgcgggac gagggcgaga aagcgcgcaa gcaggtgttg gaaaatgcga tgaaacagct
     7381 tgccaaaggc gcaacggcgg aagaggtttt ggaacggctg tccgtccaac tgaccaacaa
     7441 gctgctgcat tcgccaactc aaaccttgaa taaggcgggg gaagaagata aagatttggt
     7501 tcatgccgtc gcgcagattt atcatttgga caaataacgg tgcgccggaa aaggatgccg
     7561 tctgaagggg tttcagacgg catatttttg tgccgcctga caacatcgtg aaatcccaca
     7621 ttatatcgat gtaattacaa agtattatcc tatatgcgga ttggttttaa gatttgtaaa
     7681 tttgatttgc atcaaaaatc gccgatagat gattcatata atatcaatat taaagagtat
     7741 cggtatatcg gggatagcta tgtcctgttt ttcaatccgg cgtatgtctg cgtttcgggc
     7801 gcggataacg gcgtttttta ccgcctttgt ctttttgacg gcggcactgc ccgcttatgc
     7861 ggagcgtctg cctgattttc tggcgaaaat acagccttcg gaaatttttc cgggtgcgga
     7921 tcgttacggc aagccggaag gcaagcctat ggttgcccgc gtttacaaag gcgatgagca
     7981 gctcggtttg gtttatatca cgaccgatgc ggtcaatacg cgcggttatt cgagcaaacc
     8041 gatcgatacg ctgatggctt tggcaaacga cggcacgata gccggggcga aactggtcga
     8101 tcatcacgaa ccgattatgc tgatcggtat cccgcaatcg cgtgtcgata agttcatcga
     8161 caaatatatc ggtctgaatt ttattaaaaa tccgccgacc ccgggcgtgg cgccgggcga
     8221 catcatcagc ggtgcgactg ttacactgat ggtggttaac gacagcatcc agcgttcgta
     8281 caaggtcatt gccaaccaat accgtctggg ttcggacaag gcccttcaga cggcatccgc
     8341 ttccgatgtt cgggaagccg cgcctgcgtc agaaacccgt ccgcgccgta tggcaaatcc
     8401 cgacaagcag gatattttgt cttgggacga acttttgaaa caaaaggccg tcggccatct
     8461 gcatatcacg ctcgatcaaa tcaacaaact gtttgagaaa ggcggcaagg ccggcgtggc
     8521 cgatcacgcc gaacagggcg atcctgacga tacctttatt gatttgtatg ttgccttggt
     8581 cagccagcct tccatcggta aaagcctgct gggtaaggac ggctgggcgc atctgcaaaa
     8641 acggctgaaa cccgggcagc aggcggtttt ggttgccgga gagggccgtt attcttggaa
     8701 aggttcgggc tatgtgcgcg gcggtatttt cgaccgtatc gagatgattc agggggagaa
     8761 cagcttccgt tttaccgatg cccaacacga acgcgtcgtc gagctgtctg ccgccgatgc
     8821 gccgcgtttt aaagaagttt cttggtttac catccctgaa ggcgtagcgt ttgacggtgc
     8881 ggagccgtgg cggctgtaat tgatggttca gcgcgtattg agtgtgaacg ataaagcctt
     8941 cgttaccgcc gatttggatt acgaactgcc ccaagcttat tacgtcgatg atcctaaagc
     9001 gccgcccgta gaaatcagcg cgcctgtcga agcagtacct gccgcagcat ccgataccgc
     9061 ttcagacggc attgccgaag atgcgtcagc tgaaaacggt gtatccaacc aattatggaa
     9121 acagatttgg aaagcgaaac aggggcagat tgtcgtcgtc ggcattgcgc tgaccatcct
     9181 gcttttggta ttcctgttcc aagattggat tgtgcgttac gaaaaatggt acgaccgctt
     9241 ccgctttgct ttcctaactt ttacgctctt ctatatcggc tggtatgcac aggcgcagct
     9301 ttcggtcgtc aatacgctga cgctgttttc cgccatcctt accgaatttc attgggaatt
     9361 cttcctgatg gatccgattg tgttcatcct gtggctgttc acggctgcaa ccatgctgct
     9421 gtggaacagg gggacgtttt gcggttggtt gtgtccgttc ggctcattac aggaattgac
     9481 taaccgcatt gccaaaaaac tgggtgtgaa acagattacc gtgccgcata tgctgcatac
     9541 ccgtttgaac gtcataaaat acctgatttt cttcggtttt tttgcaattt cgctgtatga
     9601 tttgggtacg gcggaaaaat ttgccgaagt cgaaccgttc aaaactgcga ttatcttgaa
     9661 attcatgtgc gattggtggt ttgtcgcctt tgccgtggca cttttgattg cggggctgtt
     9721 tatcgaacgc ttcttctgcc gttatctgtg tcctttgggt gcgggcatcg cgctgccggg
     9781 cagattccgc gtgttcgact ggctgcgccg ctacaaaatg tgcggcaacc cctgccaaat
     9841 ctgtacgcac gaatgccccg ttcaggcaat cgcgcccgag ggcgacatcc atccgaacga
     9901 atgtatccag tgcctgcact gccaagtgat gtaccaccac gatacgcgct gtccgcaagt
     9961 tgtggcggaa aataagaaaa aacaaaaaca ggctgctgcc aaatcagggg aattggaaaa
    10021 tgtctctaag cagccgcagg aacaggtcgt ccgttttgtc aaacccgaaa cggcgcaatc
    10081 tgaaaaataa gcatccgggg gaagcctgaa gccaaagtgt caggcaggca gggtttcccc
    10141 ggaaggataa taaaattttt ttcccatcgg cttgccgtcc gaaaaccggt ttgccgtatt
    10201 ttatccaagg agtgttgtat gtcagacgaa aaattagaac aaaacggctt gagccgtcgt
    10261 tcgttcttag gtacggccgc cgcttccggt gcaggtattg ccggtgcggg cctgttgggt
    10321 ttggcgggtt gctctaaaga cggcgaacag gctgccgcta acgcttccgg cgcggctccc
    10381 gtcgccaagg cgcaagggga atccaaaccc ggccaactgt cttccgaagt cggtccgggc
    10441 gaactcgatc aatattacgg tttcctttcc ggcggccagt ccggcgagat gcgcctgatc
    10501 ggtctgcctt ctatgcgcga actgatgcgt atccccgtgt tcaatatgga cagcgcgacc
    10561 ggttggggac gcaccaatga gagcttgaaa gtcctcaacg gcaatattac cgaagaaacc
    10621 cgtaaattct taaaagacgg cggcctgcgc tgctacccca acggcgactt gcaccacccg
    10681 cacctgtcgt ttaccgacca aacttatgac ggccgctatg cctatgccaa cgacaaggca
    10741 aacaaccgcg tctgccgcgt gcgtttggat gtgatgaagg ccgacaaaat catcgacatc
    10801 cctaatgatt caggtattca cggtctgcgt ccgcaacgtt atccgaaaac cggttatgtt
    10861 tttgccaacg gcgaacacat tacccctgtc agcggtgtag gcaaactgga tgatgcgaaa
    10921 acctggaatg cagtgtacac cgccatcgac ggcgaaacca tggagatcgc atggcaagta
    10981 ttggttgacg gtaacctgga caacggcgat gccgactatc aaggcaaata ttcttttgcc
    11041 acctgctaca actccgagcg cgcgctgacc gtacaaggtg cgtcttccaa cgagcaggac
    11101 tggtgcgtcg ttttcgacct gaaagccatc gaagaaggca tcaaagcggg cgacttcaaa
    11161 gaagttaacg gtgtgaaaat gctggacggc cgcgctgagg ccaaatccaa atacacgcgt
    11221 tatatccctg tgccgaactc tcctcacggc tgtaacgcaa gccctgacgg taaatacatc
    11281 atgcccaacg gtaaactgcc tccaaccgtt accgtattgg atgtcagcaa actggacgat
    11341 ttgttcgccg gcaaaatcaa agagcgcgat gtggtcgtag ccgaaccgca actgggtctc
    11401 ggcccgttac acactgcatt cgacggtcgc ggcaatgctt atacgacatt gtttatcgac
    11461 agccaaatgg tgaaatggaa tattgacgat gcgatcaaag cctacaaagg cgagaaaatc
    11521 gatccgatca aacaaaaact cgacgttcac tatcaaccgg gtcacaacca tacgaccatg
    11581 ggcgaaacca aagaagccga cggtcaatgg ctggtgtcct tgaacaaatt ctccaaagac
    11641 cgcttcttga atgccggtcc tttgaaaccc gagtgcgacc aattgatcga catctccggc
    11701 gacgaaatgc gtctggtaca cgacaacccg acttttgccg aaccgcacga cttgtgtttg
    11761 gttgccgcgt ccaaactgaa cccgggcaaa acttgggacc gcaaagaccc gtggttctag
    11821 caggatgctt tggaacaggc gaaaaaagac ggtgtcgagc tggaaaaagc cgccaaagcc
    11881 gtacgcgaag gcaacaaagt acgcgtgtat atgacggccg tcgcgcctgc ttacagtatc
    11941 ccgcagtttg aagtgaacca aggcgacgaa gtaaccgttt acgttaccaa cgtcgaaacc
    12001 atcgaggacc tgacccacgg cttcactttg gaaggctgcg gcatcgctat ggaaatcggc
    12061 ccgcaagcca cttcttccgt aaccttcaaa gccgtccgcc cgggtgtgca ctggtactac
    12121 tgccaatggt tctgccacgc attgcatatg gaaatgtccg gtcagatgat tgttcatccc
    12181 aaataatgcg gcccgacggc tgaatatgga gaatggcgtc tgaaggcaac aggtcttcag
    12241 acggcattta tagccaaacc gcttgccgta ccgtttcagg cggtttcgct ataaaaacag
    12301 gcacatttga atgatgcttt attttgttcg gccataccga acgaaacaga gggacactat
    12361 gcacacatcc gcgctccgta tatggctgaa ggccgtgctg atacttgccg ccggcagcat
    12421 ttttcagacg gcatcggcag ccgtcgtcca cgtttccccg caagacaacc ttgccgaaat
    12481 ttttgcccgc gcccgggcgg gcgatacgat caaactggct tccggcgtat atcaaaccaa
    12541 actttatatc gacaaaccga ttacgattga agggcctgcc gaccgttccg caaccatcga
    12601 aggggacaag agcgggcgta ccatagccgt acacgcgccg gacgtaacgc tccgcaacct
    12661 gaccgttacc cgttccggta tgagcctgcc agcaatggat gccggtattt atctcgaaaa
    12721 agccgccccg cgccccctgg ttgaacacaa caatattttt gataattcgt tcggcgtata
    12781 ccttcatggt tctgccgatg cgatggtgcg cgagaataaa atcgtcggcg atgcgacctt
    12841 gcgcgtgaat gagcgcggca atggcgttac cgtttggaac gcgcccggcg cgcaggtcgt
    12901 cggcaacgac atttccaaag ggcgggacgg cattttttcc aataccagca cgcacaacac
    12961 ctataaaaac aaccgcttca gcgacctgcg tttcgccgtc cactatatgt acaccaacga
    13021 cagcgaagtc agcggcaata tttccgtggg caacaatatg ggctatgtgc tgatgttttc
    13081 cgaacggctc aaagtgttcg acaatatcgc cgtcggcagc cgcgattagg gcatcatgct
    13141 caactatgtc aactattccg atattcacga caatattatc aacaaagcgg gcaagtgcgt
    13201 ttttgcctac aatgccaact acgataaact gtccgccaat cattttgaaa actgccaaat
    13261 cggcatgcac tttaccgccg ccatcgaagg cacgtccctg cacgacaatt cctttatcaa
    13321 caacggaagc caggtcaaat atgtcagtac gcgctttctc gactggagcg agggcggaca
    13381 cggcaactac tggagcgaca acagcccgtt cgatttgaac ggcgacggct tcggagacag
    13441 cgcgtaccgt cccgacggca tcatcgacca aatcatctgg cgcgcgcccg tatcgcgcct
    13501 cttgatgaac agtcccgcaa tcagcatcgt caaatgggcg caggcgcagt ttcccgccgt
    13561 tctgcccggc ggcgtggtgg acagcaaacc gctgatgaag ccttatgccc ccaaaattca
    13621 aacccgttat caggcgatga aggacgagtt gctcaaagaa gccgaaacgc ggcagtcgga
    13681 acggggcagg gcggaaaacg gttctttgaa ctagtctgct tcagacggca cccggattca
    13741 aatgccgtct gaaaacacaa aaggaacaac catgaccaca catcatgtcg aattgaggaa
    13801 ggtaaccaaa cggttcgggg cgcaaaaagc cgtcagccaa gtcgatttgg ttttgaaggc
    13861 aggagaaagc gtcgggcttg ccggacacaa cggcgcgggc aagtccacca tcatgaagct
    13921 gatactcggg ctgattaccc cgaccgaagg cgaagtgatg cttttgggcg aacgtaccgg
    13981 cagcaaagcg ggggcgcggc ttcgcagcca aatcggctac ctgcccgaaa ccgttgcgct
    14041 gcacccttcg ctgaccggca tcgaaacgct ggatttttat gccaaactta aaaaacagcc
    14101 gctcacgcag aaccgggggc tgcttgagcg cgtcggcatt tcccagaccg cacaccgccg
    14161 cgtcggcact tattctaaag ggatgcgcca acgccttgcc ttggcacaag ccctgctggg
    14221 cgagcccaaa gtcctgctgt ttgacgaacc gacaaccggc cccgaccctg catcacgaca
    14281 aatgttttac gaagtcgtgc gcgaactcaa cgggcgcggc gcgaccgtat tgctcagtac
    14341 ccacgccctt gccgagttgg acggacacgc cgaccgcatt gtcgtgatga aaaacggcgt
    14401 taaggttgcc gacggcagta tggacgaatt gcacgtccaa agcgggctgc cgctgaccgt
    14461 caatatccgc ctcaatgcgc cgcgcaccct cagcagccgc tggcagccgc tttcagacgg
    14521 catttcctac cgggcgcaat gtcaggccga agaacgtatg gaactgttgg gcgaattggg
    14581 cagcctgtcc gaccttgcct atttcgacat ccacacgccg acgcttgacg aaatgtacgc
    14641 acggttcttg aaaagggagg acgtatgaac cccgtttgga ttattaccgg caaagaagcc
    14701 cgcgacagcc tgcgtaaccg ctgggtgctt gccgccgtcc tcctgcttgc cgcactcgcc
    14761 ctttctttgg gtttcctcgg cagctcgccc accggttcgg tcaaggtcga tccgctgacc
    14821 gttaccgtcg tcagcctgtc gagcctgtct attttcctga ttccgctgat tgcgatgctg
    14881 ctttcttatg acgcactgat cggcgaaatc gaacgcggta cgatggcgtt gctgttgagt
    14941 tatcccattt ggcgcaacca aatccttgcc ggcaagtttg tcggacacct catcatcctc
    15001 gcccttgcca ccacggcagg ttacggattg gcaggcatta cgctgcaact tgccaacggc
    15061 ggtttcgaca tcgccgtttg gaaacccttt gcgctgttga ttgccgccag cgtgatttta
    15121 ggtgcggcat ttctgtctat gggctacttg attagtgcaa aggtcaaaga gcgggggacg
    15181 gcggccggta tttccatcgg cgtatggttg tttttcgtcg tcatcttcga tatggcgctt
    15241 ttgggtattc tggttgccga ctcgaaacag gtcatcaccg cgcccgtcgt tgaaacggtg
    15301 ctgctgttca atcccaccga catttaccgc ctgctcaacc tgaccggtta cgaaaatacg
    15361 gctatgtatg cgggtatggc gggtttgagc ggacaaatcg gcctgaccgt ccccgttttg
    15421 ctgaccgcgc aggttttatg ggttatcatt ccgcttgttt tggcagccgg aatttttaga
    15481 aagcgacgaa tatgaagaaa accctgttgg caattgttgc cgttttcgcc ttaagtgcct
    15541 gccggcaggc ggaagaggca ccgccgcctt taccccggca gattagcgac cgttcggtcg
    15601 gacactattg cagtatgaac ctgaccgaac acaacggccc caaagcccag atttttttga
    15661 acggcaaacc cgatcagccc gtttggttct ccaccgtcaa gcagatgttc ggctatacca
    15721 agctgcccga agagcccaaa ggcatccgcg tgatttacgt taccgatatg ggcaatgtta
    15781 ccgattggac gaatcctaat gccgacacgg agtggataga tgcgaaaaaa gccttttacg
    15841 tcatcgacag cggctttatc ggcggtatgg gcgcggaaga cgcgctgccg ttcggcaaca
    15901 aggagcaggc tgaaaaattt gcaaaggata aaggcggcaa ggtcgtcggt tttgacgata
    15961 tgcccgatgc ttacattttc aagtaaatcc aaacagaaat gccgtctgaa cgttgaagcc
    16021 aaggttcaga cggcattttt tgtcataggt tcaaacgggg caggttcgaa caactgcccc
    16081 attccttatt cctgaccggg cgtaacggcg gaatgaggtt tacatcccgc cgtaattcgg
    16141 tccgcttgcg ccttcggggc aaatccaagt gatgttttgc gtcgggtctt tgatgtcgca
    16201 ggttttgcag tgcacgcagt ttgccgcgtt gatttgcagg cgcggactgc cgttttcttc
    16261 gatgatttca tatacgccgg ccggacaata gcgcgtttcg ggagaggcgt attctttata
    16321 gttcacgtct atcatcgttt gcggattgtt cagcaccaaa tggtcgggct ggttttcttc
    16381 gtgcgcgaga ttggcaagga agacgctgct caagcggtca aaggtcaaca caccgtcggg
    16441 tttcggataa tcaatcggct tgcacacggc ggcttttttg agctgctcgt tgtctttgcc
    16501 gtgatgtttc aaggtccacg gggctttgcc tctgaaaatc atctggtcga tgccggtata
    16561 aagcgagccg aggtaaacgc cccatttgaa tgacggacgg acattgcgcg cggcataaag
    16621 ctcttgatac agccagcttt gttcaaacag tttttgatag tttcccgctt ctttgccgct
    16681 gtcgaagctt tccacttctt caaagttttc caacaagggg aacacggctt cggcggcgag
    16741 catggcggat ttcatcgcgg tatgaatgcc tttgatgcgc ggcatattga ggaatcccgc
    16801 cgcatcgccg attaaaacgc cccccttgaa cgagagcttc ggcaggcttt gcaaaccgcc
    16861 ttcaatcagc gaacgcgcgc cgtaagcaat gcggcggccg ccttcaaagg ttttgcggat
    16921 ttcgggatgg gttttgaaac gttggaactc ttcaaacggc gacagatacg ggttttgata
    16981 gtccaaaccg accacgaagc cgacggcgac tttgttgtcg tcgaaatggt aaataaacgc
    17041 accgccgtag gttttgctgt ccagcggcca gcctgcgcta tgcaccacca aaccgggctg
    17101 atgctgttca gacggcactt cccaaacttc tttgatgccc aagccgtaag tttgcggctg
    17161 gctgttttgg tcgagttgga aacgttcgat gatttgcttg gaaagcgaac ctcggcaacc
    17221 ttcggcaaac agggtttgcc gcgcccaaag ctccatgccg ggctggaaac tgtcggtcgg
    17281 cccgccgtct ttgcctacac ccatattgcc ggtcgcaata cctttgaccg agccgtcttc
    17341 gtgatacagc acttcggcgg cggcaaagcc cggatagatt tccacgccca tattttccgc
    17401 ctgctccgcc aaccagcgca cgacttcgcc taagctgacg atgtagttgc cgtgattgtc
    17461 gaaattcggg gtaataggca gattgaacgc ttttttctcc gtcaggaaca acaccttgtc
    17521 ctgagttacc gtgcgcgtca gcggcgcgcc tttttctttc cagtcgggaa tcagctcatt
    17581 cagcgcaatc ggatcgataa ccgcgcccgc cagcgaatgc gcccccgcct ccgaaccctt
    17641 ctccgccacg caaacgctga tttcgcgccc gttctgttcg gcaagctgct tgagtttgat
    17701 ggcggcggac aaccccgacg ggcctgcgcc gacaatcacg acatcgtatt gcatactgtc
    17761 gcgggtgatg gattctgtca tggcggttcc tgtgtattta ttattgaatt gcaaatccgt
    17821 aattatacaa cgggaacata tagttaccaa atacaacaaa ggtcgtctga aaaccatatt
    17881 ttcggttttc agacgacctt tgtcgaaatt tcaataagca cgccaccatt ttacctgtcc
    17941 gaccgcaaac tccgtctgac gtttcggact gcgtgtgaaa aacgccttat ccccaccggc
    18001 atccctccct ttcggcacga ccgccaaaat tttgcctgcc aaatttccct cacgggtttg
    18061 ccaagcctcc aaaaactgcg ccctgctcat cgaaacatga cccggcgacg ggtcggcaag
    18121 caaaaccgta ttgccgccta tgccgcgcaa taccgaaaaa tggtcgtctt tgcggtattt
    18181 cagatacacg atgacgggga ttttcaactg cgcgagctgc tcgaaagaca gggcatagcc
    18241 cttcgcctca aaacccaaat cgggcataat gcgccgcata tcctcaaacg acgcgcgcat
    18301 ctgctcctta cccagttttt ccaacacttc ttcttccgtc agcttttgcc cgtaaaaatt
    18361 gttcaaaagc gtcgccaccg aagccgcccc gcaggaaaaa tccaaatcct gctttacaat
    18421 attgaaatcc cgccgcgctt tccaactctg caatttgatt tttccgtaaa caacaggatt
    18481 atcgttaaac atcggtgcag cattcaaacg ataagacaa
//
LOCUS       DAWTQS010000041        18494 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.041, whole genome shotgun sequence.
ACCESSION   DAWTQS010000041 DAWTQS010000000
VERSION     DAWTQS010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18494
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.041"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(339..4508)
                     /locus_tag="WNA25_001364"
     CDS             complement(339..4508)
                     /locus_tag="WNA25_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB"
                     /protein_id="HFA9824104.1"
                     /translation="MTDTAPTDTDPTENGTRKMPSEHRPAPPAKKRRPLLKLSAALLS
                     VLILAVCFLGWIAGTEAGLRFGLYQIPSWFGVNISSQNLKGTLLDGFDGDNWSIETEG
                     ADLKISRFRFAWKPSELMRRSLHITDISAGDIAIVTKPTPPKEERPPQGLPDSIDLPA
                     TVYLDRFETGKISMGKTFDKQTVYLERLNAAYRYDRKGHRLDLKAADTPWSSSSGSAS
                     VGLKKPFALDTAIYTKGGFEGETIHSTARLSGSLKDVRAELTIDGGNIRLSGKSVIHP
                     FAESLDKTLEEVLVKGFNINPSAFVPSLPDAGLNFDLTAIPSFSDGIALEGSLDLENT
                     KADFADRNGIPVRQVLGGFVIRQDGTVHIGNTSAALLGRGGIRLSGKIDTEKDILDLN
                     IGINSVGAEDVLQTAFKGRLDGSIGIGGTTASPKISWQLGTGTARTDGSLAIASDPAN
                     EQRKLVFDTVNISAGEGSLTAQGYLELFKDRLLKLDIRSRAFDPSRIDPQFPAGNING
                     SIHLAGELAKEKFTGKMRFLPGTFNGVPIAGSADIVYESRHLPRAAVDLRLGRNIVKT
                     DGGFGKKGDRLNLNITAPDLSRFGFGLAGSLNVRGHLSGDLDGGIRTFETDLSGTARN
                     LHIGKAADIRSLDFTLKGSPDTSRPMRADIKGGRLSLSGGAAVVDTAGLTLEGTGAQH
                     RIRTHAAMTLDGKPFKLDLDASGGINRELTRWKGSIGILDIGGAFNLKLQNRMTLEAG
                     AERVAASAANWQAMGGSLNLQHFSWDRKTGISAKGGARGLHIAELHNFFKPPFEHNLV
                     LNGDWDVAYGHNARGYLNISRQSGDAVLPGGQALGLNAFSLKTRFQNDRIGILLDGGA
                     RFGRINADLGIGNAFGGNMANTPLGGRITASLPDLGALKPFLPAAAQNITGSLNASAQ
                     IGGRVSSPSVNAAVNGSSNYGKINGNITVGQSRSFDTAPLGGRLNLTVADAEVFRNFL
                     PVGQTVKGSLNAAVTLGGSIADPHLGGSINGDKLYYRNQTQGIILDNGSLRSHIAGRK
                     WVIDSLKFRHEGTAELSGTVSMENSVPDVDIGAVFDKYRILSRPNRRLTVSGNTRLRY
                     SPQKGISVTGMIKTDQGLFGSQKSSMPSVGDDVVVLGEVKKEAAASLPVNMNLTLDLN
                     DGIRFSGYGADVTIGGKLTLTAQPGGNVRGVGTVRVIKGRYKAYGQDLDITKGTVSFV
                     GPLNDPNLNIRAERRLSPVGAGVEILGSLNSPRITLTANEPMSEKDKLSWLILNRAGS
                     GSSGDNAALSAAAGALLAGQINDRIGLVDDLGFTSKRSRNAQTGELNPAEQVLTVGKQ
                     LTGKLYIGYEYGISSAEQSVKLIYRLTRAIQAVARIGSRSSGGELTYTIRFDRLFGSD
                     KKDSAGNGKGK"
     gene            complement(4563..6410)
                     /locus_tag="WNA25_001365"
     CDS             complement(4563..6410)
                     /locus_tag="WNA25_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor"
                     /protein_id="HFA9824105.1"
                     /translation="MHDTRTMMIKPTALLLPALFFFPHAYAPAADLSENKAAGFALFK
                     SKSPDTESVKLKPKFPVRIDTQDSEIKDMVEEHLPLITQQQEEVLDKEQTGFLAEEAP
                     DNVKTMLRSKGYFSSKVSLTEKDGAYTVHITPGPRTKIANVGVAILGDILSDGNLAEY
                     YRNALENWQQPVGSDFDQDSWENSKTSVLGAVTRKGYPLAKLGNTRAAVNPDTATADL
                     NVVVDSGRPIAFGDFEITGTQRYPEQTVSGLARFQPGTPYDLDLLLDFQQALEQNGHY
                     SGASVQADFDRLQGDRVPVKVSVTEVKRHKLETGIRLDSEYGLGGKIAYDYYNLFNKG
                     YIGSVVWDMDKYETTLAAGISQPRNYQGNYWTSNVSYNRSTTQNLEKRAFSGGIWYVR
                     DRAGIDARLGAEFLAEGRKIPGSDVDLGNSHATMLTASWKRQLLNNVLHPENGHYLDG
                     KIGTTLGTFLSSTALIRTSARAGYFFTPENKKLGTFIIRGQAGYTVARDNADVPSGLM
                     FRSGGASSVRGYELDSIGLAGPNGSVLPERALLVGSLEYQLPFTRTLSGAVFHDMGDA
                     AANFKRMKLKHGSGLGVRWFSPLAPFSFDIAYGHSDKKIRWHISLGTRF"
     gene            6782..8011
                     /gene="sstT"
                     /locus_tag="WNA25_001366"
     CDS             6782..8011
                     /gene="sstT"
                     /locus_tag="WNA25_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFA9824106.1"
                     /translation="MAFGKSLFHAIGRVSLVRQIAAGLALGIVIGSVSPQLGLAAGLF
                     GSLFVGALKAVAPVLVFILVAATIAQHQKGNKAHIRPIIVLYLIGTFSAALTAVIAGM
                     VFPTHIVLAGAGDVSAAPPSGIVEVLKSLLMNLVANPINAIANANYIGILAWALVLGA
                     ALRNHGSDVTRQVVADLAEAVSTVVKWIIRFAPLGIFGLVSSTIAETGFGALAGYAKL
                     LAVLLGCMAFIALAVNPAIVWWKIRRNPYPLVFTCLRESGVYAFFTRSSAANIPVNMA
                     LAKKLGLHEDTYSISIPLGATVNMGGAAITITVLAMAAAHTQGIQVDFATALLLSLVA
                     TVSACGASGVAGGSLLLIPLACSLFGISNDVAMQVVAVGFIIGVIQDSAETALNSSTD
                     VLFTAAADLGRQRNRAE"
     gene            8162..9442
                     /locus_tag="WNA25_001367"
     CDS             8162..9442
                     /locus_tag="WNA25_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFA9824107.1"
                     /translation="MFKRSVIAMACIFPLSACGGGGGGSPDVKSADTPSKPAAPVVAE
                     NAGEGVLPKEKKDEEAAGGAPQADTQDATAGEGSQDMAAVSAENTGNGGAATTDNPKN
                     EDAGAQNDMPQNAAESANQTGNNQPAGSSDSAPASNPAPANGGSDFGRTNVGNSVVID
                     GPSQNITLTHCKGDPCNGDNLLDEEAPPKSEFESLSDEEKIKKYKKDGEKFTGLVAIK
                     VENNGLNKYTIIYQAQPTRSARSRRSLPAEIPLIPVNQADTLIVDGEAVSLTGHSGNI
                     FAPEGNYRYLTYGAEKLPGGSYALRVQGEPAKGEMLVGTAVYNGEVLHFHMENGRPYP
                     SGSRFAAKVDFGSKSVDGIIDSGDDLHMGTQKFKAAIDGNGFKGTWTENGGGDVSGRF
                     YGPAGEEVAGKYSYRPTDAEKGGFGVFAGKKDRD"
     gene            9442..9645
                     /locus_tag="WNA25_001368"
     CDS             9442..9645
                     /locus_tag="WNA25_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HFA9824108.1"
                     /translation="MLMPPEAQVFITAGRRVQIIKQAPEKGGIPKSGTEIAARIGGIP
                     QPAGETVGSSRNVGYFRPPFSGC"
     gene            9675..9953
                     /locus_tag="WNA25_001369"
     CDS             9675..9953
                     /locus_tag="WNA25_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824109.1"
                     /translation="MFRRPVFLTAVFQHSAGSNRQQQGDNALNPALRGGRVRAGLGKG
                     IAFPNRGGATGCALCAERVCGNGMPSEAGAFRRHCDVWGAGPVQIRIS"
     gene            complement(9946..10161)
                     /locus_tag="WNA25_001370"
     CDS             complement(9946..10161)
                     /locus_tag="WNA25_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824110.1"
                     /translation="MFKLGVYACLGLFAGWVLLLIVQLWFSFLEAELFFKITLTMAGL
                     FVIILAALLVCGQYFSEKKMKDNGFIN"
     gene            complement(10154..10369)
                     /locus_tag="WNA25_001371"
     CDS             complement(10154..10369)
                     /locus_tag="WNA25_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HFA9824111.1"
                     /translation="MKEYKVVIYQESLLSSLFFGAAKVNPVNFSAFLNKQTPEGWRVV
                     TMEKDLRRMLLFFKREAYVVILERDRV"
     gene            complement(10572..11915)
                     /gene="argG"
                     /locus_tag="WNA25_001372"
     CDS             complement(10572..11915)
                     /gene="argG"
                     /locus_tag="WNA25_001372"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFA9824112.1"
                     /translation="MNQNHTILQNLPVGQKVGIAFSGGLDTSAALLWMKLKGALPYAY
                     TANLGQPDEDDYNAIPKKAMEYGAENARLIDCRAQLAHEGIAAIQCGAFHVSTGGIAY
                     FNTTPLGRAVTGTMLVSAMKEDDVNIWGDGSTYKGNDIERFYRYGLLTNPALKIYKPW
                     LDQQFIDELGGRHEMSEFLIANGFNYKMSVEKAYSTDSNMLGATHEAKDLEFLNSGIK
                     IVKPIMGVAFWDENVEIEPEEVSVRFEEGVPVALNGKEYADPVELFLEANRIGGRHGL
                     GMSDQIENRIIEAKSRGIYEAPGMALFHIAYERLVTGIHNEDTIEQYRINGLRLGRLL
                     YQGRWFDSQALMLRETAQRWVAKAVTGEVTLELRRGNDYSILNTESPNLTYQPERLSM
                     EKVEGAAFTPLDRIGQLTMRNLDITDTRAKLGIYSQSGLLSLGEGSVLPQLGNKQ"
     gene            12040..12840
                     /locus_tag="WNA25_001373"
     CDS             12040..12840
                     /locus_tag="WNA25_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFA9824113.1"
                     /translation="MNAFNLIIIGDEILHGSRQDKHFAFFKSLLESKGLKLNQVQYLP
                     DEPDLLVRQLRRSFSDGIPTFVTGGIGATPDDRTRQTAAAALDLPVVRHPEAAKFIEG
                     ITRKRGKPLDSPEHAQRLKMADFPEGAELVPNPFNNIAGFSIREHYFFPGFPVMAHPM
                     AEWVLETYYAGRFNQTERGSRSVYVFEQPESRITPIIEHIEQTYPGVRSYSLPSVGWT
                     HSDGTQVKPHIEFGIKAEGEAVNLLDAAWAEVLHSLDGLGAKLKNRVN"
     gene            12880..13866
                     /locus_tag="WNA25_001374"
     CDS             12880..13866
                     /locus_tag="WNA25_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFA9824114.1"
                     /translation="MQYRIRRENEAPEAKNAGETLWERDIMREVLLSAYRDRRRERMW
                     KNIWRAVSTLILVALIAGIFRKDEAALQLAGNTPHTAVVNLYGKIGNGVEDQVKKLKD
                     GMEAAYKNPQAKAIVIRANSPGGSPVVSNTAFEEIRRLKAQHPGIPVYLVAEDMCASG
                     CYYIAAAADKIYADPSSIVGSIGVIGSSFDATGLMEKIGVKRRVKIAGSNKGMGDPFS
                     PETPEQSKIWEEMLTGIHGEFIKAVKTGRGGRLKFRQYPDVFSGRVYTGADALKVGLV
                     DGLGNIYSVARDVVKAPDVVDYTPKDDFGRILGRRFGAELKASVSEALQAVR"
     gene            14527..14778
                     /locus_tag="WNA25_001375"
                     /pseudo
     CDS             14527..14778
                     /locus_tag="WNA25_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(14776..14969)
                     /locus_tag="WNA25_001376"
                     /pseudo
     CDS             complement(14776..14969)
                     /locus_tag="WNA25_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor"
     gene            15071..15403
                     /locus_tag="WNA25_001377"
                     /pseudo
     CDS             15071..15403
                     /locus_tag="WNA25_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981114.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(15470..15844)
                     /locus_tag="WNA25_001378"
     CDS             complement(15470..15844)
                     /locus_tag="WNA25_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824115.1"
                     /translation="MIIQNEFNLYPSNMLPEGFCYPEKYVRISNDTSLIPYIQPHNFH
                     WWFENYGTEGAEVAYIFRNSILPDLNLIPFAGNGEWEAYFDGNDVTGNPRVIVINLDN
                     IENHEFFNSFEEWLELAIKDTW"
     gene            complement(16018..16374)
                     /locus_tag="WNA25_001379"
     CDS             complement(16018..16374)
                     /locus_tag="WNA25_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mimic protein"
                     /protein_id="HFA9824116.1"
                     /translation="MNEHNLLIFCLKDNVSISEYTEMVDWAYENIQSETVVEITENQI
                     IEYQNRGLWGLVSEITDNWLFGPSEGDWLIDKESILAVKEKLQNSDFSTEPLVKNIIH
                     VLEYAIKNEKTVIFHF"
     gene            complement(16371..17219)
                     /gene="mafB"
                     /locus_tag="WNA25_001380"
                     /pseudo
     CDS             complement(16371..17219)
                     /gene="mafB"
                     /locus_tag="WNA25_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225727.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
     gene            complement(17320..17529)
                     /locus_tag="WNA25_001381"
     CDS             complement(17320..17529)
                     /locus_tag="WNA25_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824117.1"
                     /translation="MKLIDFEGNLVKISLDKDELYIIQAIVGEIYSGVCVDCRDFEII
                     HGVEKNKVLLLDKELKKIYDTWDKC"
     gene            complement(17606..17965)
                     /locus_tag="WNA25_001382"
     CDS             complement(17606..17965)
                     /locus_tag="WNA25_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HFA9824118.1"
                     /translation="MRLKLKNYDCSDFDLEDFPQDKLENFCILLTLSIGFDESNGADY
                     FYVYIYSTEWLLSNIHRPMSLKNSIVTNRFNIEHILKLINDILEICNSTSEDKSISNL
                     AKYFDWEFDDYNLNIQD"
     gene            complement(17970..>18494)
                     /locus_tag="WNA25_001383"
     CDS             complement(17970..>18494)
                     /locus_tag="WNA25_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HFA9824119.1"
                     /translation="AGTVWGGKKVELNPAKWDWVKNTGYKKPAARHMQTVDGEMAGGN
                     RPPKSITSEGKANAATYPKLVNQLNEQNLNNIAAQDPRLSLAIHEGKKNFPIGTATYE
                     EADRLGKIWVGEGARQTSGGGWLSRDGTRQYRPPTEKKSQFATTGIQANFETYTIDSN
                     EKRNKIKNGHLNIR"
BASE COUNT         4165 a         4552 c         5047 g         4730 t
ORIGIN      
        1 gttaatccgc tatatttttc ttaaccatcc cttccaacag ccgtgcgaag gtttttttat
       61 atcagtggga aatgcaatat tctattgttt ttattgtaga attttaaaaa cagattgttg
      121 tgttccgcgt ttttgccggt ttggaagcgg tggggcgcat cagcctttta taaaggcata
      181 tcggaagcct gtataaggtt tttgaacata tcgatcctgt tcccttgcag gcggacggca
      241 gcggcggatg gcgaaaggcg ggttttcaaa tgtccggttt ggcggcgcgt ctttcagacg
      301 gcatggcggc ggagcgtgcc gcgccgtctg aaaaccgttt atttcccttt gccgtttcct
      361 gcggagtctt ttttgtccga accgaagagg cggtcgaaac gtatggtgta tgtcagctcg
      421 ccgcccgacg aacggctgcc gatacgggca accgcctgta tggcgcgggt cagccggtaa
      481 atcagtttga cggactgttc cgcgctggag atgccgtatt cgtagccgat gtagagtttg
      541 ccggtcagtt gtttgccgac ggtcagcacc tgttcggcgg ggttgagttc gccggtttgc
      601 gcgttgcggc tgcgcttgct ggtaaagccc aaatcatcca ccagcccgat gcggtcgttg
      661 atttgcccgg caagcagcgc gcctgcggct gcggacaggg cggcattgtc gccgctgctg
      721 ccgctgccgg cgcggttgag gatgagccag gagagcttgt ctttttcact catcggttcg
      781 tttgccgtca gcgtaatgcg cgggctgttg aggctgccca atatttccac gcccgcaccg
      841 acgggggaaa ggcggcgttc ggcgcggatg ttcaggttgg ggtcgttgag cgggccgaca
      901 aaggagactg tgcctttggt aatgtctaaa tcctgcccgt atgctttgta acgccctttg
      961 atgacgcgga ccgtgcccac cccacgcaca tttccgcccg gttgcgcggt cagggtcagt
     1021 ttgccgccta tggtaacgtc cgcgccgtag ccggagaagc ggatgccgtc attgaggtct
     1081 aaagtcaggt tcatattgac ggggagcgat gccgccgcct ctttcttgac ttcgcccaat
     1141 acgacgacat cgtcgccgac ggacggcatc gaggattttt gcgaaccgaa cagcccctga
     1201 tcagttttaa tcataccggt aacggatatg cctttttgcg gcgaatagcg caggcgggtg
     1261 ttgccggaaa ccgtcaggcg gcggttgggg cgggacagga tgcggtattt gtcgaacacc
     1321 gcgccgatat cgacatcggg cacgctgttt tccatgctga ccgtgccgga gagttccgcc
     1381 gtcccttcgt gccggaattt caggctgtcg attacccatt tcctgcctgc aatatgcgaa
     1441 cgcagcgagc cgttgtccaa gatgatgcct tgggtttggt tgcggtaata gagcttgtcg
     1501 ccgttgatac tgccgcccaa gtgcgggtcg gcgatgctgc cgccgagggt tacggcggca
     1561 ttcaggctgc ctttgacggt ttgtccgacc ggtaggaagt tgcggaatac ttcggcatcg
     1621 gcaacggtca ggttgagcct gccgcccaaa ggcgcggtat cgaaggagcg gctttgcccg
     1681 acggtgatat tgccgttgat tttcccgtag ttgctgctac cgttgacggc ggcattgacg
     1741 gacggagagc ttacccgtcc gccgatttgc gcggaggcat tcaggctgcc ggtaatgttt
     1801 tgcgcggcgg cgggcagaaa gggcttcaat gcgcccaagt cgggaaggga ggctgtaatc
     1861 ctgccgccga gcggtgtatt tgccatattg ccgccgaagg cgttgccgat gcccaaatcg
     1921 gcgttaatcc gtccgaaacg cgcgccgccg tcaagcagga ttccgatgcg gtcgttttga
     1981 aagcgcgttt tcagggaaaa tgcgttcaaa cccaaagcct gcccgccggg caatacggca
     2041 tcgccgcttt gccggctgat attgaggtag ccgcgcgcgt tgtgcccgta ggcaacatcc
     2101 cagtcgccgt ttaaaaccag attgtgttcg aagggcggtt tgaagaaatt gtgcaactcg
     2161 gcgatgtgca ggccgcgtgc gccgcctttt gccgatatgc cggttttcct gtcccaagaa
     2221 aagtgttgca ggttgaggct gccgcccatt gcctgccaat ttgccgcact tgccgccacg
     2281 cgttccgcac cggcttcgag cgtcatacgg ttttgcagct tgaggttgaa tgcgccgccg
     2341 atgtcgagga tgccgatgct gcctttccat cgggtaagtt ccctgttgat gccgcctgaa
     2401 gcgtccaaat cgagtttgaa cggtttgccg tccagcgtca tggcggcgtg tgtgcggatg
     2461 cggtgctgcg cgcccgtacc ttccagcgtc aggccggcgg tatcgacaac cgccgcgccg
     2521 cccgacaggg aaaggcggcc gcccttgata tcggcgcgca tcgggcggct tgtgtcgggc
     2581 gagcctttga gggtaaaatc gagcgaacgg atgtctgccg ctttgccgat gtgtaagttg
     2641 cgcgccgtgc cggaaaggtc ggtttcaaag gttcggatgc cgccgtccaa atcgccggaa
     2701 aggtgtccgc gtacatttaa agaccccgcg agtccgaaac cgaaacggga taaatcgggt
     2761 gcggtgatat tgaggttaag ccggtcgcct tttttgccga agccgccgtc tgttttgacg
     2821 atgttccgcc ccaaccgcaa atcgacggcg gcacgcggaa ggtggcggga ctcgtaaaca
     2881 atgtcggcac tgccggcaat cggcacgccg ttgaacgtac cgggcaaaaa acgcattttg
     2941 cccgtaaatt tctcttttgc cagttcaccg gcaagatgaa tcgaaccgtt gatattgcct
     3001 gccggaaatt gcggatcgat gcgcgaaggg tcgaatgcgc gggaacggat gtccagcttg
     3061 agcaggcggt ctttaaacag ctcgagatag ccttgcgcgg tcaggctgcc ttccccggcg
     3121 gagatgttga cggtgtcgaa caccagtttc cgctgttcgt ttgcggggtc gcttgcgatg
     3181 gcgaggctgc cgtccgtgcg tgccgtgccg gtgccgagtt gccaagagat tttgggcgag
     3241 gcggtcgtgc cgccgatgcc gatgctgccg tccaacctgc ctttgaacgc ggtttgcagc
     3301 acgtcttccg cgccgacgga gttgatgcct atatttaaat caaggatgtc tttttcggtg
     3361 tcgattttgc ccgacagcct gatgccgccc cgtccgagca gggcggcgga cgtattgccg
     3421 atatgcaccg tgccgtcctg ccggatgaca aagccgccca aaacctgacg gacggggatg
     3481 ccgttgcggt cggcaaagtc ggctttggtg ttttccaaat cgagcgagcc ttccagcgcg
     3541 atgccgtctg aaaacgacgg gatggcggtc aggtcgaaat tcagcccggc atcgggcagg
     3601 gaaggcacga aggcggacgg attgatgttg aatcctttga ccagtacttc ttccaatgtt
     3661 ttatccaatg attcggcaaa cgggtggatg acggattttc ccgagaggcg gatattgccg
     3721 ccgtcgatcg tcagttcggc gcgcacatcc ttcaggctgc cgctcagccg cgccgtactg
     3781 tgtatggttt cgccttcgaa tccgcctttg gtgtaaatgg cggtatcgag ggcaaacggt
     3841 tttttcaagc cgaccgaggc tgaccccgac gaactgctcc acggcgtgtc ggcggccttc
     3901 aggtcgaggc ggtgcccttt acggtcgtaa cggtatgccg cgttgaggcg ttcgagatag
     3961 acagtttgtt tgtcaaaggt tttgcccatg ctgattttgc ccgtctcgaa gcggtcgaga
     4021 tagacggtgg cgggcaggtc tatgctgtcg ggcaggcctt gaggcgggcg ttcttcttta
     4081 ggcggagtcg gtttggttac gatggcgatg tcgccggcgg agatgtcggt gatgtgcagg
     4141 ctgcggcgca tcagttcgga cggtttccac gcgaagcgga agcggctgat tttaaggtct
     4201 gccccctcgg tttctatcga ccagttgtcg ccgtcgaagc cgtcgagcag tgtgcctttg
     4261 aggttttggg aggaaatgtt tacgccgaac caggacggga tttggtacag cccgaagcgc
     4321 aaacctgctt ccgtaccggc gatccagccg aggaaacata ctgccaaaat caggacagac
     4381 agcagtgccg ccgacagctt cagcagcggg cggcgttttt ttgccggcgg ggcggggcgg
     4441 tgttcagacg gcattttgcg cgtgccgttt tcggtcggat cggtatctgt cggtgcggta
     4501 tcggtcataa tgtctgtttc aaaatggttt gctgcaatgc cgtctgaagc ggccgtatcg
     4561 gtttagaaac gcgttcccaa gctgatgtgc cagcggattt ttttgtcgct gtgtccgtag
     4621 gcgatgtcga aggaaaacgg cgcgagcggg ctgaaccagc gcacgcccag tcccgaaccg
     4681 tgtttcagct tcatacgttt gaaattggcg gcggcatcgc ccatatcgtg gaataccgcg
     4741 ccggacaggg tgcgcgtaaa cggcagttgg tattccaagc tgcccaccaa gagggcgcgt
     4801 tcgggcagga ccgatccgtt cggaccggca agcccgatgc tgtcaagttc gtaaccgcgc
     4861 acggaagacg cgccgccgct gcggaacatc agccccgagg ggacatcggc attgtcgcgt
     4921 gcaacggtgt aacccgcttg tccgcgtatg atgaacgtgc cgagtttttt gttttcgggc
     4981 gtgaagaaat aacctgcgcg ggcagaggtg cggattagcg cggtggagga caggaatgtg
     5041 cccaaagtcg tcccgatttt gccgtcgagg taatggccgt tttcggggtg cagcacgttg
     5101 ttgagcagct ggcgtttcca agaggcggtc agcatcgtgg cgtggctgtt gcccaaatcg
     5161 acatccgagc cggggatttt ccggccttct gcgagaaatt ccgcccccag cctggcatcg
     5221 atgcccgcgc ggtcgcgcac ataccagatg ccgccggaga aggcgcgttt ttcaaggttt
     5281 tgggtggtcg aacggttgta ggaaacgttg cttgtccagt agttgccctg atagttgcgc
     5341 ggctggctga tgccggcggc aagcgtggtt tcgtatttgt ccatatccca gacgaccgag
     5401 ccgatatagc ctttgttgaa gaggttgtaa tagtcgtagg cgattttgcc gcccaaaccg
     5461 tattccgaat cgaggcggat gccggtttcg agtttgtggc gtttgacctc ggttacgctg
     5521 actttgacgg ggacgcggtc gccttggagg cggtcgaagt cggcttgtac ggacgcgccg
     5581 gaataatgcc cgttttgttc gagcgcctgt tggaagtcga gcagcaggtc gaggtcgtag
     5641 ggcgtgcccg gttggaagcg cgccaggccg gagacggttt gttcggggta acgctgtgtg
     5701 ccggtgattt caaagtcgcc gaaggcgatg gggcggccgc tgtccacgac gacgttcaaa
     5761 tcggcggtgg cggtatcggg gttgacggcc gcccgggtgt tgccgagctt ggcaagcggg
     5821 tagcctttgc gcgttaccgc gccgaggacg gaagttttgc tgttttccca actgtcctga
     5881 tcgaaatcgc tgcctaccgg ctgctgccag ttttccagcg cgttgcggta gtattcggcg
     5941 aggttgccgt ctgaaaggat gtcgccgagg atggcgacgc cgacgttggc gattttggtg
     6001 cgcgggcccg gtgtgatgtg caccgtataa gctccgtctt tttccgtcag gctgaccttg
     6061 ctgctgaaat agcctttgct gcggagcatg gttttgacgt tgtccggtgc ttcttcggca
     6121 aggaatcccg tctgttcctt atccaaaacc tcttcctgct gctgcgtgat gagcggcagg
     6181 tgttcttcga ccatatcttt gatttcactg tcctgcgtgt cgatgcggac ggggaatttg
     6241 ggttttaatt tgactgattc ggtgtcgggg cttttgcttt tgaacaatgc gaaacccgcc
     6301 gccttgtttt cggaaaggtc ggcggcaggc gcgtatgcgt gcggaaagaa aaataaagcc
     6361 ggcaggagca gggcggtcgg tttgatcatc atggtacggg tgtcgtgcat cggtgtggcg
     6421 gatttcgatt ctaacactgt tcggacgggg cgggacgctt cagacggcat actgccgacg
     6481 gtatttcgga ggttgcggcc cgccggaaat gtatctgctt gttttaaggc attttgtttc
     6541 caatccgcat atggaaaaca ggctgcgttt cggaatagtg tcgaaaaata ggcaggatta
     6601 aataaattta ttctcgttca ataaattaaa ccgttgcgcc gaatgataaa acaaagcctg
     6661 tccctgcata gggcaaatca acgcacctca aaacacagct tgaaaaataa aaaaaacaca
     6721 gagaatgagt tttcttttaa aatacaacat tttttaacat atcacaacat aaggaagaat
     6781 tatggcgttc ggcaaatcct tatttcatgc gatcggccgc gtcagcctgg tcaggcagat
     6841 tgccgccggt ttggcgttgg gcatcgtaat cggttcggtt tcaccgcaac tgggcttggc
     6901 ggcaggcttg ttcggcagcc tgtttgtcgg cgcgctcaaa gcggtcgcgc cggttttggt
     6961 atttattttg gtggcggcca caatcgcgca gcaccaaaaa ggcaacaagg cgcatatcag
     7021 gccgattatc gtcctttacc tcatcggcac gttttccgca gccctgaccg ccgtcatcgc
     7081 cggtatggtt ttcccgacgc acattgtttt ggcgggcgcg ggcgatgtgt ccgccgcgcc
     7141 gccttccggc attgtggaag tgttgaaatc gctgctgatg aacctggtcg ccaacccgat
     7201 taacgcgatt gccaatgcca actatatcgg cattttggct tgggctttgg ttttgggcgc
     7261 ggcgttgcgg aatcacggtt cggacgttac gcggcaggtc gttgccgatt tggcggaagc
     7321 ggtttccacc gtcgtgaaat ggattatccg ttttgcccct ttgggtattt tcgggctggt
     7381 gtcgtccaca atcgcggaga cgggtttcgg cgcgctggcg ggttatgcga agctgcttgc
     7441 ggtgctgttg ggctgtatgg cgtttatcgc gctggcggtc aatcccgcca tcgtgtggtg
     7501 gaaaatccgc cgcaaccctt atccgctggt gtttacctgc ctgcgcgaaa gcggcgtgta
     7561 tgccttcttt acccgttctt ccgccgccaa tatccccgtg aatatggctt tggcgaaaaa
     7621 gctggggctg cacgaagaca cttattcgat ttccatcccg ttgggggcga ccgtcaatat
     7681 gggcggcgcg gcgattacga ttaccgtttt ggctatggcg gcggcgcaca cccaaggcat
     7741 acaggttgat tttgccaccg cgctgctgtt gagcctggtg gcgacggtca gcgcgtgcgg
     7801 cgcgtccggc gtggccggcg gttcgctgct gctgattccg ctggcgtgca gcctgttcgg
     7861 catcagcaac gatgtcgcca tgcaggtggt cgccgtcggc ttcatcatcg gcgtgattca
     7921 ggattcggcg gaaacggcgt tgaattcttc aaccgacgtt ttgtttaccg ctgccgccga
     7981 tttgggccga cagagaaacc gtgcggaata aaccaagcta tgccgtctga agcccgtttg
     8041 gcgttcagac ggcatatttt tacaatttta tgggcatatg gcggaaacaa taccggctgc
     8101 cttgaatatg tttcaacaca cagggcgata cataaagcgt cgccctatga aggagatgat
     8161 gatgtttaaa cgcagtgtga ttgcaatggc ttgtattttt cccctttcag cctgtggggg
     8221 cggcggtggc ggatcgcccg atgtcaagtc ggcggacacg ccgtcaaaac cggccgcccc
     8281 cgttgttgct gaaaatgccg gggaaggggt gctgccgaaa gaaaagaaag atgaggaggc
     8341 agcgggcggt gcgccgcaag ccgatacgca ggacgcaacc gccggagaag gcagccaaga
     8401 tatggcggca gtttcggcag aaaatacagg caatggcggt gcggcaacaa cggacaaccc
     8461 caaaaatgaa gacgcggggg cgcaaaatga tatgccgcaa aatgccgccg aatccgcaaa
     8521 tcaaacaggg aacaaccaac ccgccggttc ttcagattcc gcccccgcgt caaaccctgc
     8581 ccctgcgaat ggcggtagcg attttggaag gacgaacgtg ggcaattctg ttgtgattga
     8641 cggaccgtcg caaaatataa cgttgaccca ctgtaaaggc gatccttgta atggtgataa
     8701 tttattggat gaagaagcac cgccaaaatc agaatttgaa tcgttaagtg atgaagagaa
     8761 aattaagaaa tataaaaaag atggagaaaa gtttactggt ttggttgcta taaaagttga
     8821 aaataatggg ttgaataaat ataccattat ttatcaagct cagcctactc gttctgcacg
     8881 gtcgaggagg tcgcttccgg ccgagattcc gctgattccc gtcaatcagg ccgatacgct
     8941 gattgtggat ggggaagcgg tcagcctgac ggggcattcc ggcaatatct tcgcgcccga
     9001 agggaattac cggtatctga cttacggggc ggaaaaattg cccggcggat cgtatgccct
     9061 ccgtgtgcaa ggcgaaccgg caaaaggcga aatgcttgtt ggcacggccg tgtacaacgg
     9121 cgaagtgctg catttccata tggaaaacgg ccgtccgtac ccgtccggaa gcaggtttgc
     9181 cgcaaaagtc gatttcggca gcaaatctgt ggacggcatt atcgacagcg gcgatgattt
     9241 gcatatgggt acgcaaaaat tcaaagccgc catcgatgga aacggcttta aggggacttg
     9301 gacggaaaat ggcggcgggg atgtttccgg aaggttttac ggcccggccg gcgaggaagt
     9361 ggcgggaaaa tacagctatc gcccgacaga tgctgaaaag ggcggattcg gcgtgtttgc
     9421 cggcaaaaaa gatcgggatt gatgttgatg ccgcccgaag cgcaggtatt cattacggcg
     9481 ggacgacgcg ttcaaatcat caaacaagcc cccgaaaaag gcggcatccc aaaatccggc
     9541 actgaaattg ccgcgcgtat cggcggcatt ccgcagcctg ccggggaaac cgtcggcagc
     9601 agccggaatg tcgggtattt ccgcccgcct ttttcgggct gttgaaatat gaaaataaag
     9661 cataggccgt ctgaatgttc agacggcctg tttttttaac ggctgtgttt caacactccg
     9721 ccggttccaa ccggcagcag cagggagata atgccttgaa ccccgctctt cggggcggca
     9781 gggttcgggc cggtttgggg aaaggcatcg cctttccgaa tcggggcggt gcaacggggt
     9841 gcgccttatg tgccgaaagg gtttgcggca acggtatgcc gtctgaagcg ggtgcgttca
     9901 gacggcattg tgatgtttgg ggcgcgggtc cggttcaaat ccgcatcagt tgataaaccc
     9961 gttgtctttc attttctttt cggaaaaata ctggccgcat accagcaggg cggcgaggat
    10021 gatgacaaac agccctgcca tagtcagtgt gattttgaag aacaattcgg cttcgagaaa
    10081 agaaaaccaa agttgcacaa tcagcagcag cacccagccg gcaaacagtc cgagacaggc
    10141 ataaacgccg agcttaaaca cggtcccgct ccaaaatgac gacgtaggct tcgcgtttga
    10201 aaaacagcag catacggcgc aaatcttttt ccatcgttac gacccgccag ccttcggggg
    10261 tttgtttgtt gaggaacgcg ctgaaattga cggggttgac ctttgccgcg ccgaaaaaca
    10321 ggctggacaa caggctttcc tgataaatga cgactttgta ttctttcatc gtatttcctt
    10381 ttcggttgaa accccgccct ttggggcggt aggatcagac tttatttggg agggaacccc
    10441 ttccaaatca ggacgataca taggttggtg ctttatgtat cgtcctgtgt gttgaaacat
    10501 aggattcctt taatcaaggg attgcctttt caggcaatcc cttaaattgc agcgatgcaa
    10561 tgcggcgaaa tttattgctt attgcccaac tgcggcaata ccgagccttc gcccagcgag
    10621 agcaaaccgc tttgcgaata aatacccagt ttggcgcggg tgtcggtgat gtcgaggttg
    10681 cgcatcgtga gctgtccgat gcggtcgagc ggtgtgaacg ccgcaccttc gactttttcc
    10741 atactcaggc gttcgggttg gtaggtcagg ttgggcgatt cggtgttcag aatcgagtag
    10801 tcgttgccgc gccgcagttc gagggtaact tcgccggtaa cggctttggc gacccagcgt
    10861 tgggcggttt cgcgcaacat caaggcttgg ctgtcgaacc agcggccttg gtagagcaaa
    10921 cgtccgaggc gcaggccgtt gatgcggtat tgttcgatgg tgtcttcgtt gtggatgccg
    10981 gtaaccaagc gttcgtaggc gatgtggaac aatgccatgc ccggggcttc gtagatgccg
    11041 cgcgatttgg cttcgatgat gcggttttcg atttggtcgc tcatacccaa gccgtggcgg
    11101 ccgccgatgc ggttggcttc gaggaagagt tcgacggggt cggcgtattc tttgccgttc
    11161 aatgcaaccg gcacgccttc ttcaaagcgc acgctgactt cttcgggttc aatttcgacg
    11221 ttttcgtccc agaaggcaac gcccatgatg ggcttaacga ttttgatgcc cgagttcaaa
    11281 aattccaagt ctttggcttc gtgggtcgcg ccgagcatat tggaatcggt ggagtaggct
    11341 ttttcaaccg acattttgta gttgaagccg ttggcaatca gaaattcgct catttcgtga
    11401 cggccgccga gttcgtcgat aaattgctgg tcgagccagg gtttgtagat tttcagcgcg
    11461 ggattggtga gcaagccgta gcggtagaaa cgctcgatgt cgttgccttt gtaggtgctg
    11521 ccgtcgcccc agatattcac atcgtcttct ttcatggcgg aaacgagcat ggtgccggtc
    11581 acggcgcggc ccagaggcgt ggtgttgaaa taggcgatgc cgccggtgga aacgtggaac
    11641 gcgccgcatt ggatggcggc gatgccttcg tgtgccagtt gtgcgcggca gtcgatcagg
    11701 cgggcgtttt ccgcaccgta ttccattgct tttttgggaa tggcgttgta atcgtcttcg
    11761 tcgggctggc caaggttggc agtgtaggca taaggcagcg cgcctttgag tttcatccac
    11821 aacagcgcgg cagaggtatc aagaccgccg gagaaggcga tgccgacttt ctgaccgacg
    11881 gggaggtttt gtaaaatagt atggttttgg ttcatgaaaa aatcctcgat agcttgaaaa
    11941 atcaaagcca ttatcttata aatagaaaat attgtcagcc atcatgccgt ctgaacgggt
    12001 attccgccgt ttcaggcggt atttaaaacc gaaaacatta tgaacgcgtt caacctcatc
    12061 atcatcggag acgaaatctt acacggcagc cgccaagaca aacatttcgc cttttttaaa
    12121 tccctgctcg aatccaaagg gctgaagctc aatcaggtgc aatatctgcc cgacgaaccc
    12181 gatttgctgg tcaggcaact gcgccgcagc ttttcagacg gcataccgac gttcgttacc
    12241 ggcggcatcg gcgccacgcc cgacgaccgc acgcgccaaa ccgcagcggc tgctttggat
    12301 ttgcccgtcg tccgccatcc tgaagccgcc aagtttatcg aaggcataac ccggaaacgc
    12361 ggcaagccgc tcgattcgcc cgaacacgcc caacgcctga aaatggcgga tttccccgag
    12421 ggcgcagagc ttgtgcccaa cccgttcaac aacatcgccg gattttccat ccgcgagcat
    12481 tatttcttcc ccggctttcc tgtgatggcg cacccgatgg cggaatgggt tttggaaact
    12541 tattacgccg gccgcttcaa ccaaaccgaa cgcggcagcc gcagcgtgta tgtgtttgag
    12601 cagcccgaat cgcgcattac gccgattata gagcatatcg agcaaactta tcccggcgtg
    12661 cgttcgtaca gcctgcccag cgtcggttgg acgcattcag acggcacgca ggtcaaaccg
    12721 cacattgagt tcggcatcaa agccgaagga gaagccgtca accttttgga cgcggcgtgg
    12781 gcggaagtgt tgcacagctt ggacggattg ggagcaaagc tgaaaaatcg ggtaaactga
    12841 aaactgtttg cccaaatcgg acgatattta aggaaaacca tgcaataccg aatcagaaga
    12901 gaaaacgaag cacccgaagc aaaaaatgcg ggggaaacct tatgggagcg cgacattatg
    12961 cgcgaagtgc tgctgtccgc ctatcgggac aggcgcaggg agcggatgtg gaaaaacatc
    13021 tggcgcgccg tcagcaccct gattctggtt gccctgattg caggcatttt ccgaaaagac
    13081 gaagccgcat tgcagttggc gggcaacacg ccgcataccg cagtcgtcaa cctgtacggc
    13141 aaaatcggca acggcgtaga agaccaggtc aaaaaactca aagacgggat ggaggccgcc
    13201 tacaaaaacc cgcaggcaaa agccatcgtc atccgcgcca acagccccgg cggttcgccc
    13261 gtcgtctcca acaccgcttt tgaagaaata cgccgtctga aggcgcagca ccccggcatc
    13321 cccgtttatc tcgtggcaga ggatatgtgc gcgtccggct gctactacat cgcggcggcg
    13381 gcggacaaaa tctatgccga cccgtccagc atcgtcggca gcatcggcgt tatcggcagc
    13441 agtttcgacg cgaccggcct gatggaaaaa atcggcgtaa aacgcagggt taaaattgcg
    13501 ggcagcaaca aaggcatggg cgatccgttt tcgcccgaaa cgcccgaaca gtcgaaaatc
    13561 tgggaagaaa tgctgaccgg catacacggc gaatttatca aagccgtcaa aaccggacgc
    13621 ggcggccgcc tcaaattccg acaatatccc gacgtgttca gcggtcgcgt gtacacaggc
    13681 gcggacgcgc tgaaagtcgg gctggtggac ggactcggca atatttacag cgtggcgcga
    13741 gacgtggtca aagctcccga tgtggtggat tacactccga aggacgattt cggcagaatc
    13801 ttgggcagac gcttcggagc agaactcaaa gcaagcgttt cggaagctct gcaggccgtc
    13861 cggtagcgtt gtttacagta tttctaaggg aataccagaa taaaaatata cacaaaaaca
    13921 ctcacctccc cagtatgctg ccactaagtc agccgcgtta ctatttaccg tgcacttaaa
    13981 gccgcaaaag gcaggttgtc cccgggaggt tttgtgcatc ctgataccga tcggttgctg
    14041 ttgctgttgc tgttgctcaa aggacggaag gccgccgata aacgagatta cctgtttgtc
    14101 gctattgacg atttttatac tctgccattt tgccggacaa aaccgcagac agtgctgcca
    14161 agtttctgac cgaacatctg gctgacccct gcttgtacct ggttgagtac gtttactctg
    14221 acaatgatag gtaatataaa gagccgtcca acatgctttc ggtgcagctt gttatgataa
    14281 tgggattggt tggaggcttg cccgatttgc ttgtccgcag accaacggta aggcggagcg
    14341 ggttatccgt accttgatgg agatgtggca tgaggaacag tcgtttgaca gtccggaaca
    14401 tcagcaaaag gagttgtgct gctttattaa tttaggcgct gtcctagata actagggaaa
    14461 ttcaaattaa gttagaatta tccctatgag aaaagccgtc taagccggta taaacaaaat
    14521 aaactcattg ggctatttgt cgcaggtgta actgcaagaa cagcggcaga gttggtaggc
    14581 gttaataaaa atactgcagc ctatgatttt catcgtttac gatgactgat ttatcaaaac
    14641 ggtccgcatt tggaaatgtt tgatggcgaa gtagaagcag atgaaagtta ttttggcgga
    14701 caacgcaaag gtaaatgcgg tcgcggtgct gccggaaaag tcgcagtatt caggcttctc
    14761 aaacgaaatg gcaaggtttc ctttgccgtc cgttatgtac gtttcgtaac attcgaagaa
    14821 ttcctgaacc ctgcccgccg tttcggtttc cgtacccccc tgttagataa ggggggaaga
    14881 ttcgaagcgg ttgccggctt cctgccgtcc gccggcgcgt ccgtcatcac gccggcaacc
    14941 gcctttgtca tcccttgctt atctcccatg tgcgaatcct caaaaacggg caaaaaaaaa
    15001 gccctgttac ttgtagaaag taaagggcgt taatttttgt taatcgcccc ttcttaggga
    15061 cgcaatatat aaggtttata cggttactgt agcgaatact caaaccgcta ctttatttcc
    15121 tattatccgc gaacaagtga aacctgacag cattgtttat acggattgtt atcgtagcta
    15181 tgatgtattg gatgcaggcg aatttagcca tttccgtatc aatcacagca cacattttgc
    15241 cgaacgacaa aaccatatta atggaattgg gaacttttgg aaccgggcaa aacgtcattt
    15301 acgcaagttt gacggcattc ccaaagagca ttttgagccg tatttaaagg agtgcgaacg
    15361 gcgttttaac aacagtgaga taaaagttct tgttccattt taaaacaatt agtaaaatcg
    15421 agtttatcct agttgtccag gacggcctca tctctttata gatagctgct taccaagtat
    15481 ccttaattgc taattcaagc cactcttcaa aactattaaa aaattcatgg ttttctatat
    15541 tatctaaatt aataacaata accctaggat ttcctgttac atcattacca tcaaaataag
    15601 cttcccattc tccattacca gcgaatggga taagatttaa atcaggcagg atggaatttc
    15661 taaatatata agctacttct gccccttctg ttccataatt ctcaaaccac cagtgaaaat
    15721 tatgtggctg aatataaggt attaaagatg tatcgttaga gatacgaaca tacttttcag
    15781 gataacaaaa cccttcagga agcatattac taggatataa attaaattca ttttgaataa
    15841 tcatttcgtt accttagtat cataaatact accattagga aactgggtag accaatttgg
    15901 tggtgctagt ccattttcac tttgtttttt taattctaaa ttgaatagct tctccccatg
    15961 tttgcggcat ctaatgctgc ttgctctgca tcaatattac taaaaattag attagtttca
    16021 aaaatgaaaa attactgttt tttcattttt tatagcatat tcaagtacat gaataatatt
    16081 tttcactaag ggctctgtag aaaaatctga attttgtaat ttttctttta cagccaaaat
    16141 actttcctta tctattagcc aatccccctc acttggtcca aataaccaat tatcggtaat
    16201 ttcagaaaca agcccccata atccacgatt ttgatattca ataatttgat tttccgtaat
    16261 ttctacaact gtttcagatt gaatgttttc ataagcccaa tcaaccattt cagtatattc
    16321 actaattgaa acattgtctt ttaaacagaa aattaacagg ttgtgttcat tcatttgtct
    16381 aagttcctga tatgagtttg ttgttgtaaa taatcttttg cttgcttacc ttgtaaatta
    16441 ccggttttca caacattacc atttgaatca agatactgtt ttcgtgaatt atcatggatt
    16501 ctaaaatagt tgttttcgtt atcttttata attgtaattt ttccatctaa actagtgtat
    16561 cgagttttaa ctttatcagg acttaaaata ccaggtgcat tgggagttag tgttttgtgc
    16621 actgaatcaa aacttgctga actccagttg ctactaaact tcttaaaact ttgttttctt
    16681 ttttcagcgg cgctgtttgg taaagattta ataggtttat tcccacctgc catttccccg
    16741 tcaacagtct gcataggtcg ggcagcaggt ttttcatagc cggtattttt aacccaatcc
    16801 catttggtcg ggttaagttc tactttttta ccgcgccaaa ccgtacctgc ggcctctgct
    16861 acggcaaggg cagtttgggc tgttgctgtt atattcggat gggcatcagc ccattgtctg
    16921 gcggaattaa tgccgtcttt taccgcaaag gcactgtcct gtaataggct cgcgacagca
    16981 agttgtgctt ccggacttaa ttttcctaaa tcattaatac cttgtagagt ctgctgagcg
    17041 gctgtatctg tgaccgggct taccgcactg tctgtaattg ccccaatccc tactccttga
    17101 aaacccgtta aaaaaggatt aaccgcacct tggacgatgc cgcgaatatc atccatacgg
    17161 ttagcccgcc aatttttatc ggggtcgtct tcccatatca gttttcctgc ttcatccgca
    17221 cagttaggat tggcattgat gcccacccag tttcaggcag cctgaaaagg atttttaata
    17281 tacggcaaaa ggtgggcaac gagtgcccac cctatctagc taacatttat cccaagtatc
    17341 atatattttt ttaagttctt tatccaaaag aagaacttta tttttttcaa caccatgaat
    17401 tatctcaaaa tctctacaat ccacgcatac gcctgaataa atttctccta caattgcttg
    17461 aataatatac aactcatctt tatcgagact aattttaact aaattccctt caaaatcaat
    17521 taatttcatg aataattcct tttattttaa tttgtcatca aaatactgtt tacctgtttt
    17581 tggtctaaat gctgtcccac catctttagt cttggatatt tagattataa tcgtcaaatt
    17641 cccagtcaaa atattttgcc aaatttgata tagacttatc ttcagacgtt gaattacata
    17701 tttccaatat atcatttatc aattttaata tgtgttcaat attaaaacgg tttgtaacaa
    17761 ttgagttttt taatgacatc ggtctatgta tatttgaaag taaccattcg gtgctatata
    17821 tataaacata aaaataatca gcaccattac tttcatcaaa acctattgat aatgtcaaaa
    17881 gtatacaaaa attttctaac ttgtcttgtg ggaaatcttc caaatcaaag tcagaacaat
    17941 cataattttt taattttaat ctcatataat tacctaatat ttaaatgtcc atttttaatt
    18001 ttatttcttt tttcatttga atcaatagta taagtttcaa aatttgcttg aatacctgta
    18061 gttgcaaatt gtgatttttt ttctgttggt ggccgatatt gtcgagtgcc atctctactt
    18121 aaccatccgc ctccactagt ttgtcttgca ccctcaccaa cccaaatttt acctagtcta
    18181 tctgcctctt cataagttgc agttcctatt ggaaaatttt ttttaccctc atgaatagct
    18241 agactcaatc ttggatcttg agccgcaatg ttatttaagt tttgctcatt tagctgatta
    18301 accaacttag gataggttgc agcattagct tttccttccg acgttataga tttaggcggt
    18361 ctattccccc ctgccatctc cccatctaca gtctgcatat ggcgggcagc aggtttttta
    18421 tagccggtat ttttaaccca atcccatttg gccgggttaa gttctacttt tttaccgccc
    18481 caaaccgtac ctgc
//
LOCUS       DAWTQS010000042        18352 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.042, whole genome shotgun sequence.
ACCESSION   DAWTQS010000042 DAWTQS010000000
VERSION     DAWTQS010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18352
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.042"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(217..1497)
                     /gene="hemL"
                     /locus_tag="WNA25_001384"
     CDS             complement(217..1497)
                     /gene="hemL"
                     /locus_tag="WNA25_001384"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFA9824120.1"
                     /translation="MNRNEILFDRAKAIIPGGVNSPVRAFGSVGGVPRFIKKAEGAYV
                     WDENGTRYTDYVGSWGPAIVGHAHPEVVEAVREAALGGLSFGAPTEGEIAIAEQIAEI
                     MPSVERLRLVSSGTEATMTAIRLARGFTGRDKIIKFEGCYHGHSDSLLVKAGSGLLTF
                     GNPSSAGVPADFTKHTLVLEYNNIAQLEEAFAQSGDEIACVIVEPFVGNMNLVRPTEA
                     FVKALRGLTEKHGAVLIYDEVMTGFRVALGGAQSLHGITPDLTTMGKVIGGGMPLAAF
                     GGRKDIMECISPLGGVYQAGTLSGNPIAVAAGLKTLEIIQREGFYENLTALTQRLANG
                     IAAAKAHGIEFAADSVGGMFGLYFAAHVPRNYADMARSNIDAFKRFFHGMLDRGIAFG
                     PSAYEAGFVSAAHTPELIDETVAVAVEVFKAMAA"
     gene            complement(1561..1736)
                     /locus_tag="WNA25_001385"
                     /pseudo
     CDS             complement(1561..1736)
                     /locus_tag="WNA25_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244690.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
     gene            complement(2000..2074)
                     /locus_tag="WNA25_001386"
                     /pseudo
     CDS             complement(2000..2074)
                     /locus_tag="WNA25_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
     gene            2271..3599
                     /gene="miaB"
                     /locus_tag="WNA25_001387"
     CDS             2271..3599
                     /gene="miaB"
                     /locus_tag="WNA25_001387"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFA9824121.1"
                     /translation="MKKVFIRTFGCQMNEYDSEKMLSVLAEEHGGIEQVTQADEADII
                     LFNTCSVREKAQEKVFSDLGRVRPLKEKNPGLIIGVAGCVASQEGENIIKRAPYVDVV
                     FGPQTLHRLPKMIVDKETSGLSQVDISFPEIEKFDHLPPARVEGGAAFVSIMEGCSKY
                     CSFCVVPYTRGEEFSRPLNDVLTEIANLAQQGVKEINLLGQNVNAYRGEMDDGEICDF
                     ATLLRIVHEIPGIERMRFTTSHPREFTDSIIECYRDLPKLVSHLHLPIQSGSDRVLSA
                     MKRGYTALEYKSIIRKLRAIRPDLCLSSDFIVGFPGETEREFEQTLKLVKDIAFDLSF
                     VFIYSPRPGTPAANLPDDTPHEEKVRRLEALNEVIEAETARINQTMIGTVQRCLVEGI
                     SKKDPDQLQARTANNRVVNFTGTPDMINQMIDLEITEAYTFSLRGKIVEA"
     gene            3617..3781
                     /locus_tag="WNA25_001388"
     CDS             3617..3781
                     /locus_tag="WNA25_001388"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824122.1"
                     /translation="MPPEAFQTAFCLVSADGTAAVIRTSGINFQTRNGTPSTARKPPP
                     EPFLPPAGFQ"
     gene            complement(3846..5759)
                     /gene="dxs"
                     /locus_tag="WNA25_001389"
     CDS             complement(3846..5759)
                     /gene="dxs"
                     /locus_tag="WNA25_001389"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFA9824123.1"
                     /translation="MNPSPLLDLIDSPQDLRRLDKKQLPRLAGELRAFLLESVGQTGG
                     HFASNLGAVELTIALHYVYDTPEDKLVWDVGHQSYPHKILTGRKNQMHTMRQYGGLAG
                     FPKRCESEYDAFGVGHSSTSIGAALGMAATDKLLGGDRRSVAIIGDGAMTAGQAFEAL
                     NCAGDMDVDLLVVLNDNEMSISPNVGALPKYLASNVVRDMHGLLSTVKAQTGKVLDKI
                     PGAMEFAQKVEHKIKTLAEEAEHAKQSLSLFENFGFRYTGPVDGHNVENLVDVLKDLR
                     SRKGPQLLHVITKKGNGYKLAENDPVKYHAVANLPKEGGAQMPSEKEPKPAAKPTYTQ
                     VFGKWLCDRAAADSRLVAITPAMREGSGLVEFEQRFPDRYFDVGIAEQHAVTFAGGLA
                     CEGMKPVVAIYSTFLQRAYDQLVHDIALQNLPVLFAVDRAGIVGADGPTHAGLYDLSF
                     LRCVPNMIVAAPSDENECRLLLSTCYQADAPAAVRYPRGTGTGAPVSDGMETVEIGKG
                     IIRREGEKTAFIAFGSMVATALAVAEKLNATVADMRFVKPIDEELIVRLARSHDRIVT
                     LEENAEQGGAGGAVLEVLAKHGICKPVLLLGVADTVTEHGDPKKLLDDLGLSAEAVER
                     RVREWLPDRDAAN"
     gene            complement(5844..6761)
                     /gene="xerC"
                     /locus_tag="WNA25_001390"
     CDS             complement(5844..6761)
                     /gene="xerC"
                     /locus_tag="WNA25_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFA9824124.1"
                     /translation="MVLDGFAAYFDAYLENIVREGKSEHTVAAYRRDLEELFALLAQM
                     PSEDAGGVPQDLSRRDFTAALRRLSQRGLDGRTLARKLSAWRQYCAWLVKRGLMRADP
                     TADIKPPKQPERVPKALPQEWLNRMLDLPVDGGDPLAVRDHALFELMYGSGLRVSEIH
                     GLNADDVYLDEAWVHVTGKGRKQRQVPLTGKSVEALKNYLPLRQTASDGKALFTGRNG
                     TRLSQRQIQKRLESWAAQYGDGRHVSPHMMRHSYAGHLLQASRDIRAVQELLGHSSLS
                     TTQIYTKLDFDHIARLYDEAHPRAKRQDE"
     gene            6955..8019
                     /locus_tag="WNA25_001391"
     CDS             6955..8019
                     /locus_tag="WNA25_001391"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class II"
                     /protein_id="HFA9824125.1"
                     /translation="MALVSMRQLLDHAAENSYGLPAFNVNNLEQMRAIMEAADQVNAP
                     VIVQASAGARKYAGAPFLRHLILAAVEEFPHIPVVMHQDHGASPDVCQRSIQLGFSSV
                     MMDGSLLEDGKTPSSYEYNVNATRTVVNFSHACGVSVEGEIGVLGNLETGEAGEEDGV
                     GAAGKLSHDQMLTSVEDAVRFVKDTGVDALAIAVGTSHGAYKFTRPPTGDVLRIDRIK
                     EIHQALPNTHIVMHGSSSVPQEWLKVINEYGGNIGETYGVPVEEIVEGIKHGVRKVNI
                     DTDLRLASTGAVRRYLAENPSDFDPRKYLGKTIEAMKQICLDRYLAFGCEGQAGKIKP
                     VSLEKMASRYAKGELNQIVK"
     gene            8158..9015
                     /locus_tag="WNA25_001392"
     CDS             8158..9015
                     /locus_tag="WNA25_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="factor H binding family protein"
                     /protein_id="HFA9824126.1"
                     /translation="MTRSKPVNRTTFCCLSLTAGPDSDRLQQRRGGGGGVAADIGTGL
                     ADALTAPLDHKDKGLKSLTLEASIPQNGTLTLSAQGAEKTFKAGGKDNSLNTGKLKND
                     KISRFDFVQKIEVDGQTITLASGEFQIYKQDHSAVVALRIEKINNPDKIDSLINQRSF
                     LVSDLGGEHTAFNQLPDGKAEYHGKAFSSDDAGGKLTYTIDFAAKQGHGKIEHLKTPE
                     QNVELASAELKADEKSHAVILGDTRYGGEEKGTYRLALFGGRAQEIAGSATVKIGEKV
                     HEIGIADKQ"
     gene            9154..9828
                     /gene="tsaB"
                     /locus_tag="WNA25_001393"
     CDS             9154..9828
                     /gene="tsaB"
                     /locus_tag="WNA25_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFA9824127.1"
                     /translation="MQADFNRPVLAVDTGTSYLSLALRADGEIRLFHQEVGIRQSELI
                     LPEIRTLFRNAGITAADLGAIVYAKGPGAFTGLRIGIGVAQGLATPFDTPLIGIPTLD
                     AAASLPPPQSCILAAADARMGEVFYAWFDTLNRRRLSDYQVGRAADIALPEGYVFSDG
                     IGSAFALENRPPFSGKPDMPTAADFLALALGGYPATGAAHAELLYVRNKIALTAKEQA
                     ERKART"
     gene            9825..10265
                     /gene="rimI"
                     /locus_tag="WNA25_001394"
     CDS             9825..10265
                     /gene="rimI"
                     /locus_tag="WNA25_001394"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFA9824128.1"
                     /translation="MNIRPALPSDCAALAALDTVCNPSAWTQRQFESALVSPSEQVFL
                     AEKDGRIAAFIVWQNLPDESELHLIATAPECRRRGVASALLEYWYAHLPEGTQRLLLE
                     VRAGNAAAQALYTKHGFSIAGRRKNYYCAADGQTEDAVLMEKIC"
     gene            10259..10996
                     /locus_tag="WNA25_001395"
     CDS             10259..10996
                     /locus_tag="WNA25_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HFA9824129.1"
                     /translation="MLSARYLHLHEALGLGPMWLKQAAAVLPPKNTPATPAQARPQTV
                     RAATIRPSQPHNVQARLETMKALETAAVHTRKPAPETETPPPGLSDGIAPVPAASGIT
                     KLAVVSLCPPIEDAVYGQLFHGKAGILLDNILKAAGLDAAYVHKTCWVKTAAVGNPMP
                     SEQAVADALGQIAGELDGCRAPAVLFLGQAFVNLERQAMIETLCGSRPFFIIDHPARL
                     LRQPELKARNWQVLKQLKRALRQGGGS"
     gene            11058..11699
                     /gene="pyrE"
                     /locus_tag="WNA25_001396"
     CDS             11058..11699
                     /gene="pyrE"
                     /locus_tag="WNA25_001396"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFA9824130.1"
                     /translation="MTDFRQDFLKFSLAQNVLKFGEFTTKAGRRSPYFFNAGLFNDGA
                     STLQLAKFYAQSIIESGIRFDMLFGPAYKGIILAAATAMMLAEKGVNVPFAYNRKEAK
                     DRGEGGVLVGAPLKGRVLIIDDVISAGTSVRESIKLIEAEGATPAGVAIALDRMEKGT
                     GELSAVQEVEKQYGLPVAPIASLNDLFILLQNNPEFGQFLEPVRTYRRQYGVE"
     gene            11773..12201
                     /locus_tag="WNA25_001397"
     CDS             11773..12201
                     /locus_tag="WNA25_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824131.1"
                     /translation="MPACFCPHCKTRLWVKETQLNVAQGFVVCQKCEGLFKAKDHLAS
                     TKEPIFNDLPEAVSDVKLVHRIGTHAISKKQISRDEIADILNGGTTLHDTPPATAAAA
                     PAAAPQVSVPPARQEGLNWTIATLFALIVLIMQLSYLFIL"
     gene            12198..13508
                     /gene="argA"
                     /locus_tag="WNA25_001398"
     CDS             12198..13508
                     /gene="argA"
                     /locus_tag="WNA25_001398"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFA9824132.1"
                     /translation="MNAPDSFVAHFREAAPYIRQMRGTTLVAGIDGRLLEGGTLNKLA
                     ADIGLLSQLGIRLVLIHGAYHFLDRLAAAQGRTPHYCRGLRVTDETSLGQAQQFAGTV
                     RSRFEAALCGSVSGFARAPSVPLVSGNFLTARPIGVIDGTDMEYAGVIRKTDTAALRF
                     QLDAGNIVWMPPLGHSYGGKTFNLDMVQAAASVAVSLQAEKLVYLTLSDGISRPDGTL
                     AETLSAQEAQSLAEHAASETRRLISSAVAALEGGVHRVQILNGAADGSLLQELFTRNG
                     IGTSIAKEAFVSIRQAHSGDIPHIAALIRPLEEQGILLHRSREYLENHISEFSILEHD
                     GNLYGCAALKTFAEADCGEIACLAVSPQAQDGGYGERLLAHIIDKARGIGISRLFALS
                     TNTGEWFAERGFQTASEDELPETRRKDYRSNGRNPHILVRRLHR"
     gene            13604..15619
                     /locus_tag="WNA25_001399"
     CDS             13604..15619
                     /locus_tag="WNA25_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HFA9824133.1"
                     /translation="MKSYPDPYRHFENLDSAETQNFAAEANAETRARFLNNDKARALS
                     DGILNQMQDTRQIPFCQEHRARMYHFHQNAEYPKGVYRVCTAATYRSGYPEWKILFSV
                     ADFDELLGDDVYLGGVSHLVEQPNRALLTLNKSGGDTAYTLEVDLEAGELVEGGFHFP
                     AGKNHVSWRDENSVWVCPAWDERQLTESGYPREVWLVERGKSFEESLPAYQIDKGAMM
                     VNAWRYLDPQGSPIDLIEASDGFYTKTYLQVSSEGGAKPLNLPNDCDVVGYLAGHLLL
                     TLRKDWHRANQSYPSGALVAVKLNRGELGAAQLLFAPDETQALESVETTKRFVVASLL
                     ENVQGRLKAWRFADSKWQEAELPHLPSGALEMTDQPWGGDVVYLAASDFTTPLTLFAL
                     DLNVMELTVMRLQPQQFVSDGIEVRQFWAVSSDGERIPYFHVGKNAAPDTPTLVYAYG
                     GFGIPELPHYLGSVGKYWLEEGNAFVLANIRGGGEFGPRWHQAAQGISKHKSVDDLLA
                     VVRDLSERGMSSPKHIGLQGGSNGGLITAAAFVREPQSIGALVCEVPLTDMIRYPLLS
                     AGSSWTDEYGNPQKYEACKRRLGELSPYHNLSDGIDYPPALITTSLSDDRVHPAHALK
                     FYAKLRETSPQSWLYSPDGGGHTGNGTQRESADKLACVLLFLKEFLG"
     gene            complement(15732..16688)
                     /locus_tag="WNA25_001400"
     CDS             complement(15732..16688)
                     /locus_tag="WNA25_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFA9824134.1"
                     /translation="MNTAAIYRQYQTYVRSDKSGWALDGCSDSALIAQAKQPGLHLKM
                     CINRFDSGITLSRMRGGGTGAFPTEIHNFSHNCALFVMVSGQNRLQMGGREYRPSAGE
                     IWLVRGELADVSETLLPDSGGMCALHLDFSLEKLRRWHDEGLLDERLFSPQTIGRFAL
                     QRLAQNAGTLTAAACPLLQRPFESDGFGLLADEADALELSARLLRFTFRRHDNGYRRR
                     RIDEAADILNSEFARPLTIAEIARRVGLNECYLKRYFKAQTGETVAGRLRRLRLEHAL
                     ALIESGSTIQAAMHFCGYRHAGRFNEAFRRHYGFLPSDVKKC"
     gene            16924..17895
                     /locus_tag="WNA25_001401"
     CDS             16924..17895
                     /locus_tag="WNA25_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFA9824135.1"
                     /translation="MKPRFYWAACAVLPAACSPEPAAEKTVSAASQAASTPVATLTVP
                     TARGDAVVPKNPERVAVYDWAALDTLTEPGVNVGATTAPVRVDYLQPAFDKAATVGTL
                     FEPDCESLHRHNPQFVITGGPGAEAYEQLAKNATTIDLTVDNGNIRTSGEKQMETLSR
                     IFGKEARVAELNAQIDALFAQKREAAKGKGRGLVLSVTGNKVSAFGTQSRLASWIHGD
                     IGLPPVDESLRNEGHGQPVSFEYIKEKNPGWIFIIDRTAAIGQEGPAAVEVLDNALVC
                     GTNAWKHKQIIVMPAANYIVAGGARQLIQAAEQLKAAFEKAEPVAAQ"
     gene            17928..18251
                     /locus_tag="WNA25_001402"
     CDS             17928..18251
                     /locus_tag="WNA25_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003768951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFA9824136.1"
                     /translation="MQNETINLKQHLAAIKEYWQPEIINRHGFQFRLVKLLGDYGWHT
                     HGYSDKVLFAVEGDMAVDFADGGNMTIREGEMAVVPKSVSHRPRSENGCSVVLIDLPD
                     PSEAA"
BASE COUNT         4118 a         5592 c         5010 g         3632 t
ORIGIN      
        1 tttcagacgg catttttaca gtagattgaa atcaaaaatg tacacgcccg tcattcccgc
       61 gcaggcggga atccggaagg tcgggcttgc cgttattttc aatcattaca gaaactgaaa
      121 ggtccggatt cccgcctgcg cggaaatgac ggcggtgtgc attcttactt caatctgcca
      181 aacaaatcga acagagaaac tctgtccgtc aaaacatcat gcagccatcg ccttgaacac
      241 ttcaaccgca accgcaaccg tttcgtcaat cagctcgggc gtatgcgcgg cggaaacgaa
      301 acctgcttca taagcggacg ggccgaaggc aatgccgcgg tcgagcatgc cgtggaagaa
      361 gcgtttgaaa gcgtcgatat tggagcgcgc catatcggca tagtttcgcg gcacgtgtgc
      421 ggcgaaatac agaccgaaca taccgcccac gctgtcggcg gcaaactcga taccgtgcgc
      481 tttggcggcg gcaataccgt tggcaaggcg ttgtgtcaag gcggtcaggt tttcatagaa
      541 gccttcgcgc tggatgattt ccagcgtttt caagccggcg gcgacggcaa tcgggttgcc
      601 tgataatgta cctgcctgat acacgccgcc caacggggaa atacattcca tgatgtcttt
      661 gcgtccgccg aacgcggcaa gcggcatacc gccgccgatg actttgccca tcgtggtcag
      721 gtcgggcgtg atgccgtgca gcgactgcgc gccgccgagc gcgacgcgga aaccggtcat
      781 cacttcgtcg taaatcaaca ccgcgccgtg tttttcggtc aatccgcgca aggctttgac
      841 aaaggcttcg gtcgggcgga cgaggttcat attgccgacg aagggttcga caatcacgca
      901 ggcgatttcg tcgccgcttt gggcaaaggc ttcttcgagt tgggcgatgt tgttgtattc
      961 gagtaccaaa gtatgtttgg taaagtcggc aggcacaccg gcggaagaag gattgccgaa
     1021 ggtaagcaga ccgctgcctg ctttcaccaa caggctgtcg gaatggccgt ggtagcagcc
     1081 ttcaaatttg atgattttgt cgcggccggt aaaaccgcgt gccagacgga tggcagtcat
     1141 cgtcgcttcc gtgccggagc tgacgaggcg cagccgttcg acagacggca taatttcggc
     1201 aatttgttcg gcaatggcga tttcgccttc ggtgggcgcg ccgaacgaca aaccgcccaa
     1261 cgcagcttcg cgcacggctt cgacgacttc gggatgcgcg tgtccgacaa tcgcaggccc
     1321 ccaagagccg acataatcgg tgtagcgcgt gccgttttcg tcccaaacat acgcgccttc
     1381 ggcttttttg atgaagcgcg gcacgccgcc gacgctgccg aatgcgcgca cgggcgaatt
     1441 cacgccgccg gggatgatgg ctttggcgcg gtcgaataaa atttcgttac ggttcatatt
     1501 tatcctcaaa tgccgtctga acggcaggtt tcgggcttgg aagcagaaag ccccatttta
     1561 tcatttttca ggttgcgaca aggatttgcc cgcttctttg cggattacgc caaccgtatc
     1621 ccggatgacg gaacgctcgt ctttttcctc tttatatgtg taaagcggta gtcccggacg
     1681 actccctccc cgtcgtaatc cacgcaccat tcccaatgcc gccgctccga tttcatatag
     1741 atgaaattgg tcggcaaaaa attataaatc ggcaggctga cctcgtgata ggcataacag
     1801 ccgaaagggt tgcgcttccc gaaacgtgcc tctacctccg cccgggtcgt tttgccttta
     1861 acaaccgttt gtgcgattcc ctcttccgtc tgatatagtg gattaacaaa aatcaggaca
     1921 aggcgacgaa gccgcagaca gtacaaatag tacggcaagg cgaggcaacg ctgtactggt
     1981 ttaaatttaa tccactataa cgcaggaact gatgttccct gtcgccgaaa ttgctggtac
     2041 acgcacacag cagcaatgcc gcccatacac acacctcccg ttaaagccaa acattataca
     2101 gccgttccga ccgattaaat tcatatttta aaacaatatt ttgcctccca aacccacatc
     2161 gtgctataat ccgcaccgat tttcagacgg catcgtcgtg ccgtctgaaa ttttttcatt
     2221 ccaacaacca tcagccccgc gattacggct gcccgagaaa gacacaaacc atgaaaaaag
     2281 tatttatccg caccttcggc tgccagatga acgagtacga cagcgaaaaa atgctgtccg
     2341 tccttgccga agaacacggc ggcatcgaac aggttaccca agccgacgaa gccgacatca
     2401 tcttgttcaa cacctgttcc gtgcgcgaaa aagcacagga aaaagtgttc tccgacttgg
     2461 ggcgcgtccg tccgctcaaa gaaaaaaacc ccggcctcat catcggcgtt gccggctgcg
     2521 tcgcctcgca agaaggcgaa aacatcatca aacgcgcgcc ttatgttgac gtggttttcg
     2581 gcccgcaaac gctgcaccgc ctgcccaaaa tgattgtgga caaagaaacc agcgggctgt
     2641 cgcaagtcga tatttccttc cccgaaatcg aaaaattcga ccacctgccg cccgcccgcg
     2701 tcgaaggcgg cgcggcattt gtgtcgatta tggaaggctg ttccaaatac tgctccttct
     2761 gcgtcgtccc ctacacgcgc ggcgaagaat tctcccgccc gctcaacgac gtattgaccg
     2821 aaatcgccaa ccttgcccag caaggcgtga aagaaatcaa cctcttggga caaaacgtca
     2881 atgcctatcg cggcgaaatg gacgacggcg aaatctgcga cttcgccacc ctgctgcgta
     2941 tcgtccacga aatccccggc atcgaacgta tgcgcttcac caccagccac ccgcgcgagt
     3001 ttaccgactc gattatcgag tgctaccgcg acctgcccaa actggtttcc cacctgcacc
     3061 tgccgattca aagcggttcc gaccgcgtat tgagcgcgat gaaacgcggc tacaccgctt
     3121 tggaatacaa atccatcatc cgcaaactgc gcgccatccg tcctgatttg tgcctgagca
     3181 gcgacttcat cgtcggtttc cccggcgaga ccgaacgcga gttcgagcaa accttgaaac
     3241 tggtgaaaga cattgccttc gacttgagct tcgtgtttat ttacagcccg cgccccggca
     3301 cgcctgccgc caacctgccg gacgacacgc cgcacgaaga aaaagtgcgc cgcctcgaag
     3361 ccttgaacga agtcatcgaa gccgaaaccg cccgcatcaa ccaaaccatg atcggcacgg
     3421 ttcaacgctg cctggtcgaa ggcatctcca aaaaagaccc cgaccagctc caagcccgta
     3481 ccgccaacaa ccgcgtcgtc aactttaccg gcacgcccga catgatcaac caaatgatcg
     3541 atttggaaat caccgaagcc tacacctttt ccctgcgcgg caaaattgtc gaagcctaaa
     3601 accctcacgc cgaaaaatgc cgcctgaagc gtttcagacg gcattttgcc ttgtatcggc
     3661 agacggcacc gccgccgtta tacggacaag cggaataaat ttccaaacgc gcaacggcac
     3721 accaagcacc gcccgaaagc cgccgcccga acccttcctg ccgcctgcgg ggtttcaata
     3781 aaaaaaccgc agaaacaaac gccgtttaaa aacgccgccc gataacgacg gcgcggtcaa
     3841 gcggtttaat ttgccgcatc acggtccggc agccactcgc gcacccggcg ttccaccgct
     3901 tcggcactca aacccaaatc gtccaaaagt tttttcggat cgccgtgttc ggttacggta
     3961 tcggcaacgc ccaaaagcaa aacgggtttg cagatgccgt gtttcgccaa cacttccaag
     4021 accgcgccgc ctgcgccgcc ctgttcggcg ttttcttcaa gggtaacgat gcggtcgtgg
     4081 cttcgggcaa ggcggacaat caactcttcg tctatcggtt tgacgaagcg catatcggcg
     4141 acggtggcgt tcagtttttc ggcaaccgcc aatgcggtgg cgaccatact gccgaaggca
     4201 atgaaggcgg ttttctcacc ttcgcggcgg ataatgccct tgccgatttc cacggtttcc
     4261 atgccgtctg aaaccggcgc gcccgtaccc gtgccgcgcg gatagcggac ggcggcgggc
     4321 gcatccgcct gatagcaggt cgaaagcagc aggcggcatt cgttttcatc gctcggcgcg
     4381 gcaacaatca tgttcggcac acagcgcaag aagctcaaat cgtacaagcc ggcatgggtc
     4441 gggccgtccg cgccgacgat gcccgcacgg tcgacggcaa acaaaacggg caggttttgc
     4501 agggcgatgt cgtgcaccag ttggtcgtag gcgcgttgta aaaaggtgga ataaatcgcc
     4561 acgacgggct tcatgccttc gcacgccaaa ccgccggcaa aggtaacggc gtgctgctcg
     4621 gcgatgccga catcgaaata gcggtcgggg aatcgttgtt caaactccac cagtccgctg
     4681 ccctcgcgca tggcgggggt aatcgcaacc agtcgggaat ctgccgccgc ccggtcgcac
     4741 agccatttgc cgaatacttg ggtataggtc ggtttggcgg cgggcttggg ttctttttca
     4801 gacggcattt gcgccccgcc ttctttaggc aggttggcga cggcgtggta tttgacgggg
     4861 tcgttttcgg cgagtttgta gccgttgccc tttttggtga tgacgtgcag caactgaggg
     4921 cctttgcggc tgcgcaagtc tttcaatacg tccaccagat tctcgacgtt gtgtccgtcc
     4981 acggggccgg tgtagcggaa gccgaaattt tcaaacagcg acagcgactg tttggcgtgt
     5041 tcggcttctt cggcaagggt tttgattttg tgttcgactt tttgggcaaa ctccatcgcg
     5101 ccgggtattt tgtctaatac cttgcccgtt tgcgctttga cggtactcaa cagtccgtgc
     5161 atatcgcgca cgacgttgct ggcaagatat ttgggcaacg cgccgacgtt gggggaaatc
     5221 gacatttcgt tgtcgttgag gacgaccagc aaatccacat ccatatcgcc cgcgcaattc
     5281 aaggcttcaa acgcctgccc cgccgtcatc gcgccgtctc cgatgatggc gacgctgcgg
     5341 cggtcgccgc ccaagagttt gtccgtcgcc gccatgccca aagccgcgcc gatggaggtg
     5401 gaggaatgcc ccacgccgaa cgcgtcgtac tcggactcgc aacgtttcgg aaaacccgcc
     5461 aaaccgccgt attggcgcat ggtgtgcatc tggtttttcc tgcctgtcag gattttgtgc
     5521 gggtagcttt ggtgtccgac atcccacacc agcttgtctt cgggcgtgtc gtacacatag
     5581 tgcagggcga tggtcagttc gaccgcaccc agattgctgg cgaaatgccc gccggtctgc
     5641 ccgacagatt ccagcagaaa ggcgcgcaac tcgccggcaa ggcgcggcag ctgttttttg
     5701 tccaggcggc gcaaatcttg cgggctgtca atcaggtcga gtagggggct tgggttcatg
     5761 gtgtgtcttt tttatgtgtc gtccgggtgc aacggtcaat tatatatcaa gagcgtgcgg
     5821 ctgacggctg attttgccgt atgtcattcg tcctgccgct tggcgcgcgg gtgggcttcg
     5881 tcatagaggc gggcgatgtg gtcgaaatcg agcttggtat aaatctgcgt ggtcgaaagg
     5941 ctgctgtgtc cgagcagctc ctgcaccgcc ctgatgtcgc gcgaagcctg caacaggtgg
     6001 ccggcgtagc tgtggcgcat catatgcggc gaaacgtgcc tgccgtcgcc gtattgcgcc
     6061 gcccacgatt cgaggcgttt ttggatttgg cgttggctca ggcgcgtgcc gttcctgccg
     6121 gtaaacaggg ctttgccgtc cgatgccgtc tgacgcagcg gcagatagtt tttcaaggct
     6181 tccacgcttt tgccggtcag cggaacctga cgctgcttgc gccctttgcc ggtaacgtgt
     6241 acccacgctt cgtccaaata tacatcatct gcattcaagc cgtgtatctc gctcacgcgc
     6301 aaaccgctgc cgtacatcag ctcgaacagc gcgtggtcgc gcaccgccag cgggtcgccg
     6361 ccgtccacgg gcaaatccaa catccggttc agccattcct gcggcagggc tttgggtacg
     6421 cgctcgggct gcttcggcgg tttgatgtcg gcggtcgggt cggcgcgcat cagcccgcgt
     6481 ttgaccagcc aggcgcaata ctgccgccac gccgacagct tgcgcgccag cgtccgaccg
     6541 tccaaaccgc gctgcgacag ccgccgcaac gccgccgtaa aatcgcgccg cgacaagtcc
     6601 tgcggcacgc cgcctgcatc ttcagacggc atttgtgcca acagtgcaaa cagttcttcc
     6661 aaatcgcgcc ggtatgccgc aaccgtgtgc tccgacttgc cctcgcgcac gatgttttcc
     6721 aaataagcgt caaaatacgc cgcaaacccg tccaaaacca taaccgtccc acacaaatat
     6781 caaaaaacca gtgaaaacat tatcataaac aacacaccaa aatcccgaac cgcccaaaat
     6841 ccgccccatc tgcccgccgt tatcgttaat ataaaatcaa tgagccgttt atggtttttt
     6901 gccgtaaaaa acattataat ccgccttatt tacctattgc ccaaggagac acaaatggca
     6961 ctcgtatcca tgcgccaact gcttgaccac gccgccgaaa acagctacgg cctgcccgcg
     7021 ttcaacgtca acaacctcga acaaatgcgc gccattatgg aagccgccga ccaagtcaac
     7081 gcgcccgtca tcgtacaggc gagcgcaggt gcgcgcaaat acgcgggcgc gccgtttttg
     7141 cgccacctga ttctggcggc agtcgaagaa tttccgcaca tccccgtcgt gatgcaccaa
     7201 gaccacggcg catcgcccga cgtgtgccaa cgctccatcc aactgggctt ctcctccgtg
     7261 atgatggacg gctctttgct cgaagacggc aaaacccctt cttcttacga atacaacgtc
     7321 aacgccaccc gtaccgtcgt caacttctcc cacgcctgcg gcgtgtccgt cgaaggcgaa
     7381 atcggcgtat tgggcaacct cgaaaccggc gaagcaggcg aagaagacgg agtgggcgcg
     7441 gcaggcaaac tctcacacga ccaaatgctc accagcgttg aagatgccgt gcgtttcgtt
     7501 aaagataccg gcgttgacgc attggcgatt gccgtcggca ccagccacgg cgcatacaaa
     7561 ttcacccgtc cgcccacagg cgacgtattg cgtatcgacc gcatcaagga aatccaccaa
     7621 gccctgccca atacacacat cgtgatgcac ggctccagct ccgttccgca agaatggctg
     7681 aaagtcatca acgaatacgg cggcaatatc ggcgaaacct acggcgtgcc ggttgaagaa
     7741 atcgtcgaag gcatcaaaca cggcgtgcgc aaagtcaaca tcgataccga cctgcgcctc
     7801 gcttccaccg gcgcggtacg ccgctacctt gccgaaaacc cgtccgactt tgatccgcgc
     7861 aaatacttgg gcaaaaccat tgaagcgatg aagcaaatct gcctcgaccg ttatcttgcg
     7921 ttcggttgcg aaggtcaggc aggcaaaatc aaacctgttt cgttggaaaa aatggcaagc
     7981 cgttatgcca agggcgaatt gaaccaaatc gtcaaataac aggttgcctg taaacaaaat
     8041 gccgtctgaa cgccgttcgg acggcatttg atttttgctt ctctgacctg cctcattgat
     8101 gcagtatgca aaaaaagata ccataaccaa aacgtttata tattatctat tctgtgtatg
     8161 actaggagta aacctgtgaa ccgaactacc ttctgctgcc tttctttgac cgccggccct
     8221 gattctgacc gcctgcagca gcggaggggc ggaggcggtg gtgtcgccgc cgacatcggc
     8281 acggggcttg ccgatgcatt aaccgcgccg ctcgaccata aagacaaagg tttgaaatcc
     8341 ctaacattgg aagcctccat tccccaaaac ggaacactga ccctgtcggc acaaggtgcg
     8401 gaaaaaactt tcaaagccgg cggcaaagac aacagcctca acacgggcaa actgaagaac
     8461 gacaaaatca gccgcttcga cttcgtgcaa aaaatcgaag tggacggaca aaccatcaca
     8521 ctggcaagcg gcgaatttca aatatacaaa caggatcact ccgccgtcgt tgccctacgg
     8581 attgaaaaaa tcaacaaccc cgacaaaatc gacagcctga taaaccaacg ctccttcctt
     8641 gtcagcgatt tgggcggaga acataccgcc ttcaaccaac tgcctgacgg caaagccgag
     8701 tatcacggca aagcattcag ctccgacgat gccggcggaa aactgaccta taccatagat
     8761 ttcgccgcca aacagggaca cggcaaaatc gaacacctga aaacacccga gcagaatgtt
     8821 gagcttgcct ccgccgaact caaagcagat gaaaaatcac acgccgtcat tttgggcgac
     8881 acgcgctacg gcggcgaaga gaaaggcact taccgcctcg cccttttcgg cggccgcgcc
     8941 caagaaatcg ctggctcggc aaccgtgaag ataggggaaa aggttcacga aatcggcatc
     9001 gccgacaaac agtagtattt gaaaaatgcc gtccgaacac gataatttac cgttcggacg
     9061 gcatttttgc gccataccgc cctaggacac gacgacacgg caggcaagcc gatttcacta
     9121 tataatgccg tctgaaccaa cgagaaccac accatgcaag ccgatttcaa ccgccccgtc
     9181 ctcgccgtcg ataccggaac ttcctatttg tcgctcgcgc tgcgcgccga cggcgaaatc
     9241 cgtctgttcc atcaggaagt cggcatccgc cagtccgaac tgatcctgcc ggaaatccgc
     9301 accctattcc gaaatgcggg cattaccgcc gccgatttgg gggcgattgt ttatgcgaaa
     9361 ggccccggcg cgtttaccgg actgcgtatc ggcatcggcg tggcgcaagg tttggcaacg
     9421 ccgttcgaca cgcctttaat cggcatcccc acgctcgatg ccgccgcctc gctgccgccg
     9481 ccgcaaagct gcatactcgc cgctgcggac gcgcgtatgg gcgaagtgtt ttatgcgtgg
     9541 ttcgacacgc tgaaccgccg ccgtttgagc gattatcaag tcggtagggc ggcagacatt
     9601 gccctccccg aggggtacgt cttttcagac ggcatcggca gtgcgttcgc actggaaaac
     9661 cgtccgccct tctcaggcaa acccgatatg ccgaccgccg ccgattttct cgcgctcgcc
     9721 ctcggcggct atcccgcaac tggcgcggca cacgccgaac tgctctacgt ccgcaacaaa
     9781 atcgccctga ctgccaaaga acaggcggaa cggaaggcac gaacatgaac atccgcccag
     9841 ccttaccgtc cgactgcgcc gcactggccg cgctcgatac cgtctgcaac ccgtccgcgt
     9901 ggacgcaacg ccaatttgag tccgcgcttg tttcgccgtc cgaacaggtt ttccttgcgg
     9961 aaaaagacgg caggattgcc gcctttatcg tttggcagaa cctgcccgac gaatccgaac
    10021 tgcacctgat tgccaccgcg cccgaatgcc gccgacgggg cgttgcgtcc gccctgctcg
    10081 aatattggta cgcacatctg cccgaaggca cgcaacgcct gctgctcgaa gtccgcgcag
    10141 gcaacgccgc cgcacaggca ctgtatacca aacacggctt cagcattgcg ggcaggcgga
    10201 aaaactatta ctgcgccgcc gacgggcaaa ccgaagatgc cgtcttaatg gagaaaatat
    10261 gttaagcgcg cgctacctcc acctgcacga ggctttgggc ttgggtccga tgtggctgaa
    10321 acaggccgcc gccgtcctgc cgcccaaaaa cacacccgca accccggcac aggcacgtcc
    10381 ccaaaccgtc cgcgccgcca cgatccgccc ttcccaaccc cataacgttc aggcgcggct
    10441 cgaaacgatg aaagcgttgg aaaccgccgc cgtacatacg cgcaaacccg cgcctgaaac
    10501 cgaaacgccc ccgcccggcc tttcagacgg catcgccccc gttcccgccg cttcgggcat
    10561 taccaagctt gccgtcgtca gcctgtgtcc gccgatcgag gatgcggttt acgggcaact
    10621 gttccacggc aaagcgggca tcctgctcga caacatcctc aaagccgcag gactggatgc
    10681 cgcctatgtc cacaaaacct gttgggtgaa aaccgccgcc gtcggtaacc cgatgccgtc
    10741 tgaacaggcc gtcgcggatg cgctgggtca aatcgccggg gaactcgacg gctgccgcgc
    10801 ccccgccgtc ctattcctcg gacaggcgtt tgtcaacctg gaacggcagg cgatgattga
    10861 aactttgtgc ggcagccgtc ccttcttcat catcgaccat cccgcccggc tgttgcgcca
    10921 acccgaactc aaagcccgaa actggcaggt gttgaaacag ttgaaacgcg ccttgcggca
    10981 aggcggcggc agttgaagcg cgccgcacgg ggcggtagaa tcgcaactgc gtcccaatat
    11041 ctgacagaaa gcacaaaatg actgatttcc gccaagattt cctcaaattc tccctcgccc
    11101 aaaatgtttt gaaattcggc gaatttacca ccaaagccgg acggcggtcg ccctatttct
    11161 tcaatgccgg cctcttcaac gacggcgcgt ccacgctgca actggcaaaa ttctatgcac
    11221 aatccatcat tgaaagcggc atccgattcg atatgctgtt cggccccgcc tacaaaggca
    11281 ttattttggc ggcggcaacc gcgatgatgc tggcggaaaa aggcgtgaac gtcccgtttg
    11341 cctacaaccg caaagaagcc aaagaccgcg gcgaaggcgg cgtgttggtc ggcgcgccgc
    11401 ttaaagggcg cgtgctgatt atcgacgacg tgatttccgc cggcacatcc gtacgcgaat
    11461 caatcaaact gattgaagcg gagggtgcaa cccccgccgg tgtcgccatc gcgctcgacc
    11521 gcatggaaaa aggcacgggt gaattgtccg ccgttcagga agtggaaaaa caatacggcc
    11581 tgcccgtcgc ccccatcgcc agcctgaacg atttgtttat cctgttgcaa aacaaccccg
    11641 aattcggaca gttcctcgaa cccgtccgca cctaccgccg gcagtacggc gtagaataaa
    11701 aataaagcat atgccgtccg aaccacctgc cgcttcagac ggcatcaaac cgacacacac
    11761 cgagaaaaca tcatgcccgc ctgtttctgt ccgcactgca aaacccgcct ctgggtcaaa
    11821 gaaacccagc tcaacgtcgc ccaaggcttc gtcgtctgcc aaaaatgcga agggctgttt
    11881 aaagccaaag accatctggc aagcacgaaa gaacctatat tcaacgattt gcccgaagct
    11941 gtttcggatg tcaaactcgt ccaccgcatc ggcacgcacg ccattagcaa gaaacagatt
    12001 tcccgcgacg aaatcgccga tatcctcaac ggcggtacaa ccctgcacga tacgccgccc
    12061 gcaaccgccg ctgccgcacc tgccgccgca ccgcaggttt ccgtaccgcc cgcccgtcag
    12121 gaagggctca actggactat tgcaaccctg ttcgcactta tcgtcctcat tatgcagctt
    12181 tcctacctct tcatcctatg aacgcgcccg acagctttgt cgcccacttc cgcgaagccg
    12241 ccccctacat ccgccaaatg cgcggcacga cactggtcgc cggcatagac ggccgcctgc
    12301 tcgaaggcgg caccttaaat aagctcgccg ccgacatcgg gctgttgtcg caactgggca
    12361 tccgactcgt cctcatccac ggcgcgtacc acttcctcga ccgcctcgcc gccgcgcaag
    12421 gccgcacgcc gcattattgc cggggtttgc gcgttaccga cgaaacctcg ctcggacagg
    12481 cgcagcagtt tgccggcacc gtccgcagcc gttttgaagc cgcattgtgc ggcagcgttt
    12541 caggattcgc gcgcgcgcct tccgtcccgc tcgtatcggg caacttcctg accgcccgtc
    12601 cgataggcgt gattgacgga accgatatgg aatacgcggg cgttatccgc aaaaccgaca
    12661 ccgccgccct ccgtttccaa ctcgacgcgg gcaatatcgt ctggatgccg ccgctcgggc
    12721 attcctacgg cggcaaaacc ttcaatctcg atatggtgca ggccgccgct tccgtcgccg
    12781 tctcgcttca ggccgaaaaa ctcgtttacc tgaccctttc agacggcatt tcccgccccg
    12841 acggcacgct cgccgaaacc ctctcggcac aggaagcgca atcgctggcg gaacacgccg
    12901 ccagcgaaac ccgacgactg atttcgtccg ccgttgccgc gctcgaaggc ggcgtgcatc
    12961 gcgtccaaat cctcaacggg gccgccgacg gcagcctgct gcaagaactc ttcacccgca
    13021 acggcatcgg cacgtccatt gccaaagaag ccttcgtctc catccggcag gcgcacagcg
    13081 gcgacatccc gcacatcgcc gccctcatcc gcccgctgga agaacagggc atcctattgc
    13141 accgcagccg cgaatacctc gaaaaccaca tttccgaatt ttccatcctc gaacacgacg
    13201 gcaacctgta cggctgtgcc gcactcaaaa cctttgccga agccgattgc ggcgaaatcg
    13261 cctgccttgc cgtctcgccg caggcacagg acggcggcta cggcgaacgc ctgcttgccc
    13321 acattatcga taaggcgcgc ggcataggca taagcaggct gttcgcactg tccacaaata
    13381 ccggcgaatg gtttgccgaa cgcggctttc agacggcatc ggaagacgag ctgcccgaaa
    13441 cgcggcgcaa agactaccgc agcaacggac gaaacccgca tattctggtg cgtcgcctgc
    13501 accgctgacc gcaacggaaa gccgccgcag aaatgccgcc tgaaccccgt ttcagacggc
    13561 atttccccga ttataaagac ctgcccgacc ctcaaggacc ccgatgaaat cctaccccga
    13621 cccctaccgc cattttgaaa acctcgattc cgccgaaacg caaaacttcg ctgctgaagc
    13681 gaatgccgaa acgcgcgcgc gttttttaaa caacgacaag gcgcgcgcac tttcagacgg
    13741 cattttgaat caaatgcagg acacgcggca gattccgttt tgtcaggaac accgcgcgcg
    13801 gatgtaccat ttccatcaga atgcggaata tccgaagggc gtgtaccgcg tgtgtacggc
    13861 ggcgacctac cgttccggct atcccgagtg gaaaatcctg ttttcggtgg cggatttcga
    13921 tgagttgctc ggcgacgatg tgtatttggg cggcgtgtcg cacttggtgg agcagcccaa
    13981 ccgcgcgctg ctgactttga acaaatcggg cggcgatacg gcgtatacgc tggaagtgga
    14041 tttggaagca ggggaattgg tagagggcgg ttttcacttt ccggcaggca aaaaccatgt
    14101 gtcgtggcgc gatgaaaaca gcgtgtgggt gtgtccggct tgggacgaac gccagttgac
    14161 cgaatcgggc tatccgcgcg aagtgtggct ggtggaacgc ggcaagagtt tcgaggaaag
    14221 cctgccggcg taccaaatcg ataaaggcgc gatgatggta aacgcgtggc gttacctcga
    14281 tccgcagggt tcgccgattg atttgattga agcgtcggac ggtttttaca ccaagacgta
    14341 tttgcaggtg tcgtccgaag gcggggcgaa accgttgaac ctgcctaatg attgcgatgt
    14401 ggtcggctat ctggcgggac atcttttgct gacgctgcgc aaggactggc accgcgcgaa
    14461 ccaaagctat ccgagtggcg cgttggtggc ggtgaaactg aatcggggcg aactcggggc
    14521 ggcgcagctt ttgtttgcgc ccgatgaaac gcaggcattg gaaagcgtgg aaacgaccaa
    14581 gcgttttgtg gtggcaagcc tgctggagaa tgtacaaggc cgtctgaaag cgtggcggtt
    14641 tgccgacagc aaatggcagg aagccgagtt gccgcacctg ccctcgggcg cgttggaaat
    14701 gaccgaccaa ccgtggggcg gcgacgtggt ttatcttgcc gccagcgatt tcaccacgcc
    14761 gctgacgctg tttgcgctgg atttgaacgt gatggaactg accgtcatgc gcctccagcc
    14821 gcagcagttt gtttcagacg gcatcgaagt gcggcagttt tgggcggtgt cgtccgacgg
    14881 cgaacgcatt ccttatttcc acgtcggcaa aaacgccgcg cccgacacgc cgaccttagt
    14941 ctatgcttac ggaggtttcg gcattcctga attgccgcat tatctgggca gcgtcggcaa
    15001 atattggctg gaagagggca atgcctttgt attggcaaac atccgcggcg gcggagaatt
    15061 cggcccgcgc tggcatcagg cggcgcaggg aatcagcaaa cacaaaagcg ttgatgattt
    15121 gttggcagtc gtgcgtgatt tgtccgaacg cggcatgagt tcgcccaaac acatcggctt
    15181 gcagggcggc agcaacggcg gcctgattac cgccgccgcc ttcgtgcgcg aaccgcaaag
    15241 catcggtgcg ctggtgtgcg aagtaccgct gaccgatatg atccgttatc cgctgctgtc
    15301 cgccggttca agttggacgg acgaatacgg caatccgcag aaatacgaag cctgcaaacg
    15361 ccggctgggc gaattgtcgc cgtatcacaa tctttcagac ggcatcgatt atccgcccgc
    15421 actcattacc accagcctca gcgacgaccg cgtccatccc gcccacgcgc tcaaattcta
    15481 cgccaaactg cgcgaaacct cgccgcaatc ttggctctac tcgcctgacg gcggcggcca
    15541 taccggcaac ggcacccaac gcgaatccgc cgacaaactc gcctgcgtgt tgctgttttt
    15601 gaaagaattt ttgggataag ggcagggggc gacactgccg ccgcgaatga aaaaaggtcg
    15661 tctgaaattg ctttttcaga cgaccttttt taatggttgt ttcaaatcaa aatatctatg
    15721 ccgtcggccc atcagcactt tttcacatcc gaaggcaaaa atccgtaatg ccgcctgaac
    15781 gcctcgttga accgtcccgc gtggcggtag ccgcaaaagt gcatcgcggc ttggatggtg
    15841 ctgcccgatt cgatgagggc gagcgcgtgt tccagccgca ggcggcgcag gcgtccggcg
    15901 acggtttcgc cggtttgcgc tttgaaatag cgtttcaggt agcattcgtt cagcccgacg
    15961 cggcgggcga tttcggcgat ggtcagcggg cgggcgaatt cgctgttcaa aatatcggcg
    16021 gcttcgtcta tgcgccggcg gcggtagccg ttgtcgtggc ggcggaaggt gaagcgtaat
    16081 aatcgggcgg agagttccag cgcgtcggct tcgtcggcga gcaggccgaa gccgtccgat
    16141 tcgaacgggc gttgcagcag ggggcaggcc gccgccgtca gcgtccccgc gttttgcgcc
    16201 agccgttgca gggcgaatcg gcctatcgtt tgcggcgaaa acaggcgttc gtccagtaaa
    16261 ccttcgtcgt gccagcggcg cagtttttcc agcgaaaaat ccaaatgcag cgcacacatg
    16321 ccgccgctgt ctggcagcag ggtttcggat acgtccgcca gttcgccgcg taccagccag
    16381 atttcgccgg cagacgggcg gtattccctg ccgcccattt gtaaccggtt ctgccccgat
    16441 accatgacga acaaggcgca gttgtggctg aaattgtgga tttcggtggg aaacgcaccc
    16501 gttccgccgc cgcgcatccg tgataaggtg atgcccgagt cgaagcggtt gatgcacatt
    16561 ttcagatgca aaccgggctg ttttgcctgc gcaatgagtg cgctgtcgga acagccgtcc
    16621 aacgcccagc cggatttatc ggagcggaca taggtttggt actggcggta gatggcggcg
    16681 gtgttcatga ttggatagga acgagttgtc taacaaatga attaaatagg aattattacc
    16741 aataatcaag cacagggatt ggttggaacg gaaaaggtcg tctgaaaggg tgtttcagac
    16801 gaccttttcc gtttcgggaa tttgttttgc cgtatcggga attttgcgtt ttgcggcgtg
    16861 gtttctgcag gttgtttgct taataataaa cattcttatt cgtatgcaaa ggaaccgcac
    16921 accgtgaaac cgcgttttta ttgggcagcc tgcgccgtcc tgccggccgc ctgttcgccc
    16981 gaacctgccg ccgaaaaaac tgtatccgcc gcatcccaag ccgcatccac acctgtcgcc
    17041 acgctgaccg tgccgaccgc gcggggcgat gccgttgtgc cgaagaatcc cgaacgcgtc
    17101 gccgtgtacg actgggcggc gttggatacg ctgaccgagc cgggcgtgaa tgtgggcgca
    17161 accaccgcgc cggtgcgcgt ggactatttg cagcctgcat ttgacaaggc ggcaacggtg
    17221 gggacgctgt ttgagcccga ttgcgaatcc ctgcaccgcc acaatccgca gtttgtcatt
    17281 accggcgggc cgggtgcgga agcgtatgaa cagttggcga aaaacgcgac caccatagat
    17341 ttgacggtgg acaacggcaa tatccgcacc agcggcgaga agcagatgga gaccctgtcg
    17401 cggattttcg gtaaggaagc gcgcgtggcg gaattgaatg cgcagattga cgcgctgttc
    17461 gcccaaaagc gcgaagccgc caaaggcaaa ggacgcgggc tggtgctgtc ggttacaggc
    17521 aacaaggtgt ccgccttcgg cacgcaatcg cggttggcaa gttggataca cggcgacatc
    17581 ggcctgccgc ccgtggacga atctttacgc aacgaggggc acgggcagcc cgtttccttc
    17641 gaatacatca aagagaaaaa ccccggctgg attttcatca tcgaccgcac cgccgccatc
    17701 gggcaggaag ggccggctgc cgtggaagtg ttggataacg cgctggtatg cggcacgaac
    17761 gcttggaagc acaagcaaat catcgtcatg cctgccgcga actacattgt cgcgggcggc
    17821 gcgcggcagt tgatacaggc ggcggaacag ttgaaggcgg cgtttgaaaa ggcagaaccc
    17881 gttgcggcgc agtagtttta ttcaaaacaa accacaagga gaaccccatg caaaacgaaa
    17941 ccatcaacct gaaacagcac cttgccgcca tcaaagaata ctggcagccc gaaatcatca
    18001 accgccacgg gttccaattc cgcctggtca aacttttggg cgattacggc tggcacacgc
    18061 acggatacag cgacaaagtg ctgtttgccg tggagggcga catggcggtg gacttcgccg
    18121 acggcggcaa catgacgata cgcgagggcg agatggcggt cgtgccgaag tcggtgtcgc
    18181 accgcccgcg ttcggaaaac ggctgctcgg tggtgctgat tgatctgccc gacccgtccg
    18241 aggccgcctg aaaacgaagt ttccgaagga agctgagttt ctgcgaagct aaaagcagcc
    18301 tacaccttca atatgccaaa agcatagcgg attaacaaaa atcaggacaa gg
//
LOCUS       DAWTQS010000043        18331 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.043, whole genome shotgun sequence.
ACCESSION   DAWTQS010000043 DAWTQS010000000
VERSION     DAWTQS010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18331
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.043"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            6..81
                     /locus_tag="WNA25_001403"
     tRNA            6..81
                     /locus_tag="WNA25_001403"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Trp,seq:cca)
     gene            196..474
                     /gene="secE"
                     /locus_tag="WNA25_001404"
     CDS             196..474
                     /gene="secE"
                     /locus_tag="WNA25_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFA9824137.1"
                     /translation="MTEHTPEKKNVKVNQLVVQDKESASNSGKEGFFAYFSNSWSEFK
                     KVVWPKREDAVRMTVFVIVFVAVLSIFIYAADTAISWLFFDVLLRREG"
     gene            476..1012
                     /gene="nusG"
                     /locus_tag="WNA25_001405"
     CDS             476..1012
                     /gene="nusG"
                     /locus_tag="WNA25_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFA9824138.1"
                     /translation="MSKKWYVVQAYSGFEKNVQRILEERIAREEMGDYFGQILVPVEK
                     VVDIRNGRKTISERKSYPGYVLVEMEMTDDSWHLVKSTPRVSGFIGGRANRPTPISQR
                     EAEIILQQVQTGIEKPKPKVEFEVGQQVRVNEGPFADFNGVVEEVNYERNKLRVSVQI
                     FGRETPVELEFSQVEKIN"
     gene            1113..1547
                     /gene="rplK"
                     /locus_tag="WNA25_001406"
     CDS             1113..1547
                     /gene="rplK"
                     /locus_tag="WNA25_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFA9824139.1"
                     /translation="MAKKIIGYIKLQIPAGKANPSPPVGPALGQRGLNIMEFCKAFNA
                     ATQGMESGLPIPVVITAFADKSFTFVMKTPPASILLKKAAGLQKGSSNPLTNKVGKLT
                     RAQLEEIAKTKEPDLTAADLDAAVRTIAGSARSMGLDVEGVV"
     gene            1547..2242
                     /gene="rplA"
                     /locus_tag="WNA25_001407"
     CDS             1547..2242
                     /gene="rplA"
                     /locus_tag="WNA25_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFA9824140.1"
                     /translation="MAKVSKRLKVLRSSVEANKLYAIDEAIALVKKAATAKFDESVDV
                     SFNLGVDSRKSDQVIRGSVVLPKGTGKTTRVAVFTQGVNAEAAKEAGADVVGFEDLAA
                     EIKAGNLNFDVVIASPDAMRIVGQLGTILGPRGLMPNPKIGTVTPNVAEAVKNAKAGQ
                     VQYRTDKAGIVHATIGRASFAEADLKENFDALLDAIVKAKPAAAKGQYLKKVAVSSTM
                     GLGVRVDTSSVNN"
     gene            2470..2970
                     /gene="rplJ"
                     /locus_tag="WNA25_001408"
     CDS             2470..2970
                     /gene="rplJ"
                     /locus_tag="WNA25_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFA9824141.1"
                     /translation="MSLNIETKKVAVEEISAAIANAQTLVVAEYRGISVSSMTELRAN
                     ARKEGVYLRVLKNTLARRAVQGTSFVELADQMVGPLVYAASEDAVAAAKVLHQFAKKD
                     DKIVVKAGSYNGEVMNAAQVAELASIPSREELLSKLLFVMQAPVSGFARGLAALAEKK
                     AGEEAA"
     gene            3028..3399
                     /gene="rplL"
                     /locus_tag="WNA25_001409"
     CDS             3028..3399
                     /gene="rplL"
                     /locus_tag="WNA25_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFA9824142.1"
                     /translation="MAITKEDILEAVGSLTVMELNDLVKAFEEKFGVSAAAVAVAGPA
                     GAGAADAEEKTEFDVVLASAGDQKVGVIKVVRAITGLGLKEAKDIVDGAPKTIKEGVS
                     KAEAEDIQKQLEAAGAKVEIK"
     gene            3590..7768
                     /gene="rpoB"
                     /locus_tag="WNA25_001410"
     CDS             3590..7768
                     /gene="rpoB"
                     /locus_tag="WNA25_001410"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFA9824143.1"
                     /translation="MNYSFTEKKRIRKSFAKRENVLEVPFLLATQIDSYAKFLQLENA
                     FDKRTDDGLQAAFNSIFPIVSHNGYARLEFVYYTLGEPLFDIPECQLRGITYAAPLRA
                     RIRLVILDKEASKPTVKEVRENEVYMGEIPLMTPSGSFVINGTERVIVSQLHRSPGVF
                     FEHDKGKTHSSGKLLFSARIIPYRGSWLDFEFDPKDLLYFRIDRRRKMPVTILLKALG
                     YNNEQILDIFYDKETFYLSSNGVQTDLVAGRLKGETAKVDILDKEGNVLVAKGKRITA
                     KNIRDITNAGLTRLDVEQESLLGKALAADLIDSETGEVLASANDEITEELLAKFDING
                     VKEITTLYINELDQGAYISNTLRTDETAGRQAARVAIYRMMRPGEPPTEEAVEQLFNR
                     LFFSEDSYDLSRVGRMKFNTRTYEQKLSEAQQNSWYGRLLNETFAGAADKGGYVLSVE
                     DIVASIATLVELRNGHGEVDDIDHLGNRRVRSVGELTENQFRSGLARVERAVKERLNQ
                     AESENLMPHDLINAKPVSAAIKEFFGSSQLSQFMDQTNPLSEVTNKRRVSALGPGGLT
                     RERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYARTNDYGFLETPYRRVIDG
                     KVTEEIDYLSAIEEGRYVIAQANADLDSDGNLIGDLVTCREKGETIMATPDRVQYMDV
                     ATGQVVSVAASLIPFLEHDDANRALMGANMQRQAVPCLRPEKPMVGTGIERSVAVDSA
                     TAIVARRGGVVEYVDANRVVIRVHDDEATAGEVGVDIYNLVKFTRSNQSTNINQRPAV
                     KAGDVLQRGDLVADGASTDLGELALGQNMTIAFMPWNGYNYEDSILISEKVAADDRYT
                     SIHIEELNVVARDTKLGAEDITRDIPNLSERMQNRLDESGIVYIGAEVEAGDVLVGKV
                     TPKGETQLTPEEKLLRAIFGEKASDVKDTSLRMPTGMSGTVIDVQVFTREGIQRDKRA
                     QSIIDSELKRYRLDLNDQLRIFDNDAFDRIERMIVGQKANGGPMKLAKGSEITTEYLA
                     GLPSRHDWFDIRLTDEDLAKQLELIKLSLQQKREEADELYEIKKKKLTQGDELQPGVQ
                     KMVKVFIAIKRRLQAGDKMAGRHGNKGVVSRILPVEDMPYMADGRPVDIVLNPLGVPS
                     RMNIGQILEVHLGWAAKGIGERIDRMLKERRKAGELREFLNKLYNGSGKKEDLDSLTD
                     EEIIELASNLRKGASFASPVFDGAKESEIREMLNLAYPSEDPEVEKLGFNDSKTQITL
                     YDGRSGEAFDRKVTVGVMHYLKLHHLVDEKMHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYENIVKGEHKIDAGMPESFNVLVK
                     EIRSLGLDIDLERY"
     gene            complement(7733..7873)
                     /locus_tag="WNA25_001411"
     CDS             complement(7733..7873)
                     /locus_tag="WNA25_001411"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824144.1"
                     /translation="MDKGTVLSDGISFLNIVGLPRIPAEQLPLCRLKPVSVAFQIDIQ
                     TQ"
     gene            7921..12096
                     /gene="rpoC"
                     /locus_tag="WNA25_001412"
     CDS             7921..12096
                     /gene="rpoC"
                     /locus_tag="WNA25_001412"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFA9824145.1"
                     /translation="MNLLNLFNPLQTAGMEEEFDAIKIGIASPETIRSWSYGEVKKPE
                     TINYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKFKGVTCEKCGVEVTLSKVRRE
                     RMGHIELAAPVAHIWFLKSLPSRLGMVLNMTLRDIERVLYFEAFVVTDPGMTPLQRRQ
                     LLTEDDYYNKLDEYGDDFDAKMGAEGIRELLRTLDVAGEIEILRQELESTGSDTKIKK
                     IAKRLKVLEAFHRSGMKLEWMIMDVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVIN
                     RNNRLKRLLELHAPDIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIK
                     GKGGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIFHKLEKQGL
                     ASTVKAAKKLVEQEVPEVWDILEEVIREHPIMLNRAPTLHRLGIQAFEPILIEGKAIQ
                     LHPLVCAAFNADFDGDQMAVHVPLSLEAQMEARTLMLASNNVLSPANGEPIIVPSQDI
                     VLGLYYMTRDRINAKGEGSLFADVKEVHRAYHTKQVELGTKITVRLREWVKNEAGEFE
                     PVVNRYETTVGRALLSEILPKGLPFEYVNKALKKKEISKLINASFRLCGLRDTVIFAD
                     HLMYTGFGFAAKGGISIAVDDMEIPKEKAALLAEANAEVKEIEDQYRQGLVTNGERYN
                     KVVDIWGRAGDKIAKAMMDNLSKQKVIDRDGNEVDQESFNSIYMMADSGARGSAAQIK
                     QLSGMRGLMAKPDGSIIETPITSNFREGLTVLQYFIATHGARKGLADTALKTANSGYL
                     TRRLVDVTQDLVVVEDDCGTSDGFVMKAVVQGGDVIEALRDRILGRVTASDVVDPSSG
                     ETLVEAGTLLTEKLVDMIDQSGVDEVKVRTPITCKTRHGLCAHCYGRDLARGKLVNAG
                     EAVGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAASQVEAKSNGTARFSSQMRYVANN
                     KGELVVIGRSCEVVIHDDIGRERERHKVPYGAILLVQDGMAIKAGQTLATWDPHTRPM
                     ITEHAGMVKFENMEEGVTVAKQTDDVTGLSALVVIDGKRRSSSASKLLRPTVKLLDEN
                     GVEICIPGTSTPVSMAFPVGAVITVREGQEIGKGDVLARIPQASSKTRDITGGLPRVA
                     ELFEARVPKDAGMLAEITGTVSFGKETKGKQRLIITDVDGVAYETLISKEKQILVHDG
                     QVVNRGETIVDGAVDPHDILRLQGIEALARYIVQEVQEVYRLQGVKISDKHIEVIIRQ
                     MLRRVNIADAGETGFITGEQVERGDMMAANEKALEEGKEPARYENILLGITKASLSTD
                     SFISAASFQETTRVLTEAAIMGKQDELRGLKENVIVGRLIPAGTGLTYHRSRHQQWQG
                     VEQETAETQVTDE"
     gene            12385..12798
                     /locus_tag="WNA25_001413"
     CDS             12385..12798
                     /locus_tag="WNA25_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824146.1"
                     /translation="MKVSQSAMNKLVIAVIASMGLTACAGGGSDSSMSVQPSVSEQLK
                     DNANVDAKDEKVIEYLKKSSLKDVPKELQAKVLKVKGDEYTGVRKQYAGKLGKGESVK
                     AMLFLDGEEPFSKEQLQKMDVYVNGKKYEGSKGGN"
     gene            12816..13445
                     /locus_tag="WNA25_001414"
     CDS             12816..13445
                     /locus_tag="WNA25_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824147.1"
                     /translation="MSEQKIEFYGADKEQNYALLKTWVYEQPYSVVRGYFGYSRKDGN
                     PIEEDGQNPEEIPFDLYLGDIRGVATDEDKLPKAGSFQYEGRAFGGNGVLSKESLDNH
                     NGVFRYTIDFDRRKGSGSIEGMEQYGKIKLEEAAIERIPYRESGSSLGLKDRVSYFGV
                     NEGVAMLEKDNEIKKYHLGIFGEAANEVAGAVSQEHKHQAVIGFGGEKK"
     gene            13685..13927
                     /locus_tag="WNA25_001415"
     CDS             13685..13927
                     /locus_tag="WNA25_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824148.1"
                     /translation="MKSKNIFFMGYLIASATFAEDIGVPVEPINVGSRAAMPSEGESL
                     ALLPFAEDVPPVRDAMPSEVPKSAAGGDVRGGRSIP"
     gene            13839..14828
                     /locus_tag="WNA25_001416"
     CDS             13839..14828
                     /locus_tag="WNA25_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HFA9824149.1"
                     /translation="MCRRFAMQCRLKFLKARQAAMFEAADLYRELLSERPDLVYPRFD
                     LGVMLFEDKQYREALVQLHRAEEVLPPDMRQLAREYIRQAEAVQAWHPSFNMNYEQTD
                     NVNNASLSRDIVINGRKWIKSEDSLPKRANGIRYELGIDRMFNMAGNHFARLGISGSG
                     VHYWNARDFSEQAFHAEVGYRYRNSRLEWGFRPFVKQNRLGNNRYTANTGIALDYSRR
                     LNEKWRSTQSFQYGRKQYHDEYLAKRYNSKTISVSGTFSYYAMSAWQLYGGISGMFDN
                     TVEKEQASRRYGVSLGTVKILDGGLGLKLGAGYTKRIFKAPATLIYNFTRRDD"
     gene            14835..14984
                     /locus_tag="WNA25_001417"
     CDS             14835..14984
                     /locus_tag="WNA25_001417"
                     /inference="COORDINATES: protein motif:HMM:NF016462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HFA9824150.1"
                     /translation="MSAALWHKKLSWKGFTPQINFRYNKNNSNMPAFYSHSGKGWFVS
                     MEKTY"
     gene            15163..15534
                     /gene="rpsL"
                     /locus_tag="WNA25_001418"
     CDS             15163..15534
                     /gene="rpsL"
                     /locus_tag="WNA25_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFA9824151.1"
                     /translation="MPTINQLVRKGRQKPVYVNKVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCKVRLTNGFEVISYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSL
                     DTAGVKDRKQARSKYGAKRPK"
     gene            15652..16122
                     /gene="rpsG"
                     /locus_tag="WNA25_001419"
     CDS             15652..16122
                     /gene="rpsG"
                     /locus_tag="WNA25_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFA9824152.1"
                     /translation="MPRRREVPKRDVLPDPKFGSVELTKFMNVLMIDGKKSVAERIVY
                     GALEQIEKKTGKAAIEVFNEAIANSKPIVEVKSRRVGGANYQVPVEVRPSRRLALAMR
                     WVRDAARKRGEKSMDLRLAGELIDASEGRGGALKKREEVHRMAEANKAFSHFRF"
     gene            16141..18246
                     /gene="fusA"
                     /locus_tag="WNA25_001420"
     CDS             16141..18246
                     /gene="fusA"
                     /locus_tag="WNA25_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFA9824153.1"
                     /translation="MARKTPISLYRNIGISAHIDAGKTTTTERILFYTGLTHKLGEVH
                     DGAATTDYMEQEQERGITITSAAVTSYWSGMAKQFPEHRFNIIDTPGHVDFTVEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYQVPRLAFVNKMDRQGANFFRVVEQMKT
                     RLRANPVPIVIPVGAEDSFTGVVDLLKMKSIIWNEADKGTTFTYGDIPAELVETAEEW
                     RQNMIEAAAEASEELMDKYLGGEDLAEEEIVGALRQRTLAGEIQPMLCGSAFKNKGVQ
                     RMLDAVVELLPAPTDIPPVQGVNPNTEEADSRQASDEEKFSALAFKMLNDKYVGQLTF
                     IRVYSGVVKSGDTVLNSVKGTRERIGRLVQMTAADRTEIEEVRAGDIAAAIGLKDVTT
                     GETLCAESAPIILERMEFPEPVIHIAVEPKTKADQEKMGIALNRLAKEDPSFRVRTDE
                     ESGQTIISGMGELHLEIIVDRMKREFGVEANIGAPQVAYRETIRKAVKAEYKHAKQSG
                     GKGQYGHVVIEMEPMEPGGEGYEFIDEIKGGVIPREFIPSVDKGIRDTLPNGIVAGYP
                     VVDVRIRLVFGSYHDVDSSQLAFELAASQAFKEGMRQASPALLEPIMAVEVETPEEYM
                     GDVMGDLNRRRGVVLGMDDDGIGGKKVRAEVPLAEMFGYSTDLRSATQGRATYSMEFK
                     KYSEAPAHIAAAVTEARKG"
BASE COUNT         4954 a         3968 c         4755 g         4654 t
ORIGIN      
        1 tttagaggcc aatagctcaa ttggtagagt atcggtctcc aaaaccgagg gttgggggtt
       61 cgagaccctc ttggcctgcc aaataaaaaa ttaaccggcc ttgtgccggt taattttttt
      121 gtatttgtta tttagtaaac tctcttgcca tttacatgga ttgagaatag acagatgcta
      181 tgatggataa ataatatgac agaacatacg cctgaaaaaa agaatgttaa agtgaatcaa
      241 ctggttgttc aagataaaga atctgcatct aattccggta aggagggttt ttttgcatat
      301 ttctcaaatt cttggtccga attcaaaaag gtggtttggc ctaagcgtga agatgccgtc
      361 agaatgactg tatttgttat agtgtttgtt gctgtgcttt ctatatttat ctatgcggca
      421 gatacagcaa tttcgtggtt attttttgat gtattgctga gaagggaagg ttgagatgtc
      481 gaaaaaatgg tatgttgtac aggcgtattc ggggtttgag aagaatgtcc aacgaatatt
      541 ggaagagcga attgcccgtg aggagatggg agattatttc ggacaaattc tggtgcctgt
      601 agagaaagtt gttgatatcc gcaatggtcg taagactatt agtgaaagaa agtcatatcc
      661 tggttatgtg ttagttgaga tggaaatgac agatgactct tggcatcttg taaaaagcac
      721 tccccgtgtt tctggtttta ttggagggag ggctaataga cccacgccga ttagtcagag
      781 agaggctgaa attattttac agcaggttca gaccggcata gagaagccga aaccaaaagt
      841 tgaatttgag gttggtcaac aggttcgtgt aaatgaagga ccgtttgcgg attttaacgg
      901 ggtggttgag gaggtcaatt atgaacggaa taagttacgc gtgtctgttc agatatttgg
      961 tagagaaaca cccgttgagc tggagttcag ccaggttgag aagattaact gatttttata
     1021 cttgaaaaaa aagcaataag aggatagaat caaaaattaa cttggggagc ggaaatggtt
     1081 ccgcgtctta cccgttttta ggagtccgtt aagtggcaaa gaaaattatc ggctatatta
     1141 aactgcaaat tcctgcaggc aaagccaatc catccccccc agttggtcct gctttgggtc
     1201 aacgtggttt gaatatcatg gaattctgta aagcatttaa tgctgcaacc caaggtatgg
     1261 aatctggttt gccaattccg gttgtgatta ctgcatttgc agataaatca ttcacatttg
     1321 tgatgaaaac tcctccggct tctattctgt tgaaaaaagc cgccggtttg caaaaaggta
     1381 gttctaatcc tctgacaaac aaggtaggta aattgacccg tgcccagttg gaagaaattg
     1441 ctaaaactaa agagcctgat ttgactgctg ctgacttgga tgcggctgtt cgtactatag
     1501 caggttctgc tcgctcaatg ggcttggacg tggagggtgt tgtataatgg ctaaagtatc
     1561 taaacgcttg aaagttcttc gctcttctgt tgaagctaac aaactgtacg caatcgacga
     1621 agcaattgcc ttggttaaaa aagctgctac cgctaaattt gacgaatctg ttgacgtatc
     1681 tttcaacttg ggcgttgatt ctcgtaaatc tgaccaagtt atccgtggtt cagtcgttct
     1741 gcctaaaggt actggtaaga caacccgtgt ggctgtattt actcaaggtg taaatgcaga
     1801 agctgctaaa gaggctggtg cggatgtcgt cggtttcgaa gatttggctg ctgaaatcaa
     1861 agcaggcaat ctgaactttg atgttgttat tgcttctcct gatgcaatgc gtatcgttgg
     1921 tcaattgggt accatcttgg gtcctcgtgg tctgatgcca aaccctaaaa taggtacggt
     1981 tacccctaat gttgccgaag cggtaaaaaa tgcaaaagca ggtcaagtgc agtaccgtac
     2041 cgacaaggca ggtattgttc atgcaacgat tggtcgtgct tcgttcgctg aagctgattt
     2101 gaaagagaac tttgatgcgt tgctggacgc tatcgttaaa gccaaacctg ctgctgctaa
     2161 aggtcagtac ctgaaaaaag ttgctgtatc cagcactatg ggtttaggtg ttcgcgttga
     2221 tacatcaagc gtgaataact aatctaagaa attttcaagc aactctgttt ttgagttgct
     2281 tgaatttggg ctacttaaaa ttaagtagat gtccaagacc gtagggatcg taagatttaa
     2341 tcgtaactgc cctacgcaga cggtagtcct gaaacacatt gcaagattgc ttgtaagatg
     2401 tctttttagg ttaccgcgct ggtgggatat cgttttggta tcctgtttat aaacagtggg
     2461 aggtagacct tgagtctcaa tattgaaacc aagaaagtgg cggtcgagga aattagcgcg
     2521 gcaattgcta atgctcaaac cctcgtagtc gctgaatatc gcggtatcag tgtttccagt
     2581 atgactgagc ttcgtgcgaa tgcacgtaaa gaaggcgttt atttgcgcgt tctgaaaaat
     2641 actctggctc gtcgcgcagt gcaaggtact tcatttgtag aattggccga tcaaatggtt
     2701 ggtccgttgg tttacgctgc ttctgaagat gctgttgctg ctgctaaagt gttgcaccaa
     2761 ttcgcgaaaa aagatgacaa aattgtcgtt aaagccggtt cttacaatgg cgaagtaatg
     2821 aatgctgctc aggttgctga gttggcttct attccgagcc gcgaagagct gttgtccaaa
     2881 ctgttgttcg ttatgcaagc tcctgtatcg ggctttgcgc gcggtttggc tgctttggca
     2941 gagaaaaaag ccggcgaaga agctgcttaa tcgattttgt ttctgttaat caattatttt
     3001 ttaatacaat atttggagta aaatagcatg gctattacta aagaagacat tttggaagca
     3061 gttggttctt tgaccgtaat ggaattgaat gacctggtta aagcttttga agaaaaattc
     3121 ggtgtttctg ctgctgctgt tgcagttgca ggtcctgctg gtgccggtgc tgccgatgct
     3181 gaagaaaaaa ccgaatttga tgtcgttttg gcttctgccg gcgatcaaaa agtcggcgtg
     3241 attaaagttg tccgtgcaat tactggtttg ggtctgaaag aagctaaaga catcgttgac
     3301 ggcgcaccta aaaccattaa agagggtgtt tctaaagctg aagccgaaga catccaaaaa
     3361 caactggaag cagcaggcgc taaagtcgaa atcaaataat ttgatgcttc ttatgaaggc
     3421 tggcagtttt ctgccagcct tattttgctt cttaaaataa gcattaagta ttgtttacat
     3481 ttatttgcat agtttttatc aaatcattgc aaataaatgt aaatatcaga ttgatgcgta
     3541 ccgttgtttt cagacggcct attattgaaa attacttttc ggagtgtgta tgaactattc
     3601 gtttaccgag aaaaaacgta tccgtaagag ttttgcaaag cgggaaaatg ttttggaagt
     3661 tccttttcta ctggcaaccc aaattgattc ttatgcgaaa tttttgcagc tggaaaatgc
     3721 ttttgacaaa cgtaccgatg acggtctgca agcggcattt aattctattt tcccgattgt
     3781 gagccacaac ggttatgctc gtctggagtt tgtatattac acgctgggcg agcctttgtt
     3841 cgatatccct gaatgtcaat tgcgcggaat cacttatgct gcccctctgc gcgcgcgtat
     3901 ccgtttggtg attttggata aggaagcgtc taaaccgacg gtaaaagaag ttcgtgaaaa
     3961 cgaagtgtat atgggcgaaa ttccgttgat gaccccgagc ggttcttttg tgattaacgg
     4021 cacagagcgt gtgattgttt cccagttgca ccgttcgccc ggtgtgttct tcgagcatga
     4081 caaaggtaag acacactctt ccggcaaatt gttattctcc gcccgcatca ttccctaccg
     4141 tggttcatgg ttggattttg aatttgatcc gaaagatttg ctgtatttcc gtatcgaccg
     4201 ccgccgtaaa atgccggtaa cgattttgtt gaaggctttg ggctacaaca atgagcaaat
     4261 cttggatatt ttctacgaca aagaaacgtt ttatttgtct tcaaacggtg ttcaaaccga
     4321 tttggtcgca ggccgtctga aaggcgaaac tgccaaggtc gatatcttgg ataaagaagg
     4381 caatgtattg gttgccaaag gtaagcgcat tactgcgaaa aatatccgtg atattaccaa
     4441 tgcaggcctg acccgtttgg atgtagaaca ggaaagcctg ctgggcaaag cattggctgc
     4501 cgatttgatt gattcggaaa ccggcgaggt attggcttct gccaatgatg aaattacaga
     4561 agagttgttg gccaaatttg atattaacgg cgtaaaagaa attacgaccc tttacatcaa
     4621 tgagttggat cagggtgctt atatttccaa taccttgcgc acggatgaga ctgccggccg
     4681 gcaggcggcg cgtgttgcga tttaccgtat gatgcgtccg ggcgaaccgc ccaccgaaga
     4741 ggcggtcgag caattgttta accgcttgtt cttcagtgaa gacagctacg atttgtcccg
     4801 cgtaggccgt atgaaattta atacgcgcac atacgaacaa aaactgtccg aagcccaaca
     4861 aaactcttgg tacggccgcc tgttgaacga gacgtttgcc ggtgctgccg acaaaggcgg
     4921 ttatgtcctg agcgtcgaag atattgtcgc ctcgattgcg actttggtcg agttgcgtaa
     4981 cggccatggc gaagtggacg atatcgatca cttgggcaac cgtcgagtac ggtcggtagg
     5041 cgagctgact gaaaaccaat tccgcagcgg tttggctcgt gtggaacgtg ccgtaaaaga
     5101 acgtttgaat caggcggaat cagaaaactt gatgccgcac gacttgatta atgcgaaacc
     5161 cgtttctgcc gccatcaaag aattcttcgg ctccagccaa ttgagtcagt ttatggatca
     5221 gaccaatccc ttgtctgaag taaccaataa acgccgtgta tctgcattgg gcccgggcgg
     5281 tttgacccgc gaacgtgccg gcttcgaagt gcgggacgtg catccgaccc actatggacg
     5341 tgtatgtcct attgaaacgc ctgaaggtcc gaacatcggt ttgatcaact cattgtctgt
     5401 ttacgcgcgc accaatgatt acggtttctt ggaaacgcct taccgccgcg ttattgacgg
     5461 caaagtaacc gaggaaattg attacttgtc tgccatcgaa gaaggccgct atgtgattgc
     5521 acaggcgaat gccgatttgg attcagacgg caatctgatt ggcgatttgg ttacctgtcg
     5581 tgaaaaaggc gaaaccatta tggcaacgcc cgaccgcgtc caatatatgg acgtggcaac
     5641 cggtcaggtg gtatccgttg cggcatccct gattccgttc ttggaacacg atgacgcgaa
     5701 ccgcgcattg atgggtgcca acatgcaacg tcaggcagtg ccttgtctgc gtcctgaaaa
     5761 accgatggtc ggtaccggta tcgagcgttc cgttgccgtt gactctgcta ctgcaatcgt
     5821 tgcccgccga ggcggcgtgg tcgagtatgt cgatgccaac cgcgttgtga tccgtgtcca
     5881 tgacgacgaa gcgactgccg gcgaagtggg tgtcgatatt tacaatttgg ttaaattcac
     5941 ccgttccaat caatctacca acatcaacca acgtccggct gtcaaagcag gcgatgtttt
     6001 gcaacgcggc gatttggtgg ccgacggcgc gtccaccgat ttgggcgaat tggctttggg
     6061 tcaaaatatg accatcgcct tcatgccgtg gaacggttac aactacgaag actcgattct
     6121 gatttccgaa aaagtggctg cggacgaccg ctatacttcg attcacattg aggaattgaa
     6181 tgtcgttgcc cgcgatacca agctgggtgc ggaagacatt acccgcgata ttccgaactt
     6241 gtccgagcgt atgcaaaacc gtttggacga atccggtatc gtttacatcg gtgcggaagt
     6301 agaagccggc gatgtgttgg taggcaaggt aacgcctaaa ggcgaaaccc agctgacgcc
     6361 ggaagaaaaa ctgctgcgcg ccatcttcgg cgaaaaagcg tctgacgtaa aagatacttc
     6421 attgcgtatg cctaccggta tgagcggtac ggttatcgac gttcaagtct ttacccgcga
     6481 aggcatccaa cgcgacaaac gcgcccaatc cattatcgat tccgaattga aacgctaccg
     6541 tttggatttg aacgaccaat tgcgtatttt cgacaacgac gcattcgacc gtatcgagcg
     6601 tatgattgtc ggtcagaaag ccaacggcgg tccgatgaag ctggccaaag gcagcgaaat
     6661 cacgaccgaa tatctggcgg gtctgccgag caggcacgat tggtttgata tccgtttgac
     6721 cgatgaagat ttggccaagc agttggaact gattaaattg agcctgcaac aaaaacgcga
     6781 agaagcggat gagttgtatg agatcaagaa gaaaaaactg acccaaggcg acgagctgca
     6841 acccggcgta caaaaaatgg tgaaagtctt tatcgccatc aaacgccgtc tgcaagccgg
     6901 tgacaaaatg gcgggccgcc acggtaataa aggtgtggta tctcgcattc tgcctgtgga
     6961 agacatgccg tacatggcgg acggccgtcc tgtggacatc gtactgaacc cgttgggcgt
     7021 accttcccgt atgaacatcg gtcagatttt ggaagttcac ttgggttggg cggcaaaagg
     7081 tatcggcgag cgcatcgacc gtatgctgaa agagcgacgc aaagccggcg agctgcgcga
     7141 gttcttgaac aaactctaca acggcagcgg taagaaagaa gatttggaca gcctgactga
     7201 tgaagaaatc atcgaattgg cttccaatct gcgtaaaggt gcatctttcg cctctcctgt
     7261 attcgacggt gcgaaagagt ctgaaatccg cgaaatgttg aacttggctt acccaagcga
     7321 agatcctgag gttgagaaac tgggcttcaa cgacagtaaa actcaaatca cgctgtatga
     7381 cggccgttca ggcgaagcat ttgaccgcaa ggttacagtc ggtgtgatgc actatctgaa
     7441 actgcaccac ttggttgacg aaaaaatgca cgcgcgttct accggcccgt acagtctggt
     7501 tactcaacaa cctctgggcg gtaaagctca gtttggcggc caacgtttcg gtgagatgga
     7561 ggtttgggca ttggaagcat acggcgcggc atacacgctg caagagatgc tgactgtgaa
     7621 gtctgacgac gtgaacggcc gtaccaaaat gtacgaaaac atcgtcaaag gcgaacacaa
     7681 aatcgatgcc ggtatgcccg agtccttcaa cgtattggtc aaagagattc gctcactggg
     7741 tttggatatc gatttggaac gctactgata cgggtttcag acggcataag gggagctgtt
     7801 ctgcaggtat gcggggcagc ccgacaatgt ttaaaaacga aatgccgtct gaaagcactg
     7861 tacctttatc catatcgaaa atccgccacg cggtaaaaat atttccttca aggagcagaa
     7921 atgaatttgt tgaacttatt taatccgttg caaaccgcag gtatggaaga agagtttgat
     7981 gccatcaaaa tcggtattgc ctctcccgaa accatccgct catggtctta tggcgaagtt
     8041 aaaaaacctg aaaccatcaa ctaccgtacg ttcaaacccg agcgcgacgg cttgttctgc
     8101 gccaaaatct ttggcccggt caaagactac gaatgcttgt gcggaaaata caaacgattg
     8161 aaatttaaag gcgtaacctg tgaaaaatgt ggcgtagaag ttaccctgtc caaagtgcgc
     8221 cgcgaacgca tgggccatat cgaattggct gcacccgttg cccatatttg gttcttgaaa
     8281 tccctgcctt cccgcttggg tatggtattg aacatgacgt tgcgtgatat cgaacgcgta
     8341 ttgtactttg aagcattcgt ggtgaccgat cccggcatga ctccgttgca acgtcgtcaa
     8401 ttgctgactg aagacgatta ctacaacaaa ctggacgaat acggcgacga cttcgatgcc
     8461 aaaatgggtg cggaaggtat ccgcgaattg ttgcgaacct tggatgtagc aggcgaaatc
     8521 gaaatcctgc gccaagagct tgaatcgacc ggttctgaca ccaaaatcaa aaaaatcgct
     8581 aaacgcttga aagtattgga agccttccat cgttccggta tgaagctgga gtggatgatt
     8641 atggatgtgc tgccggtatt gccgcctgat ttgcgtccgt tggttccgtt ggacggcggc
     8701 cgttttgcca cttccgattt gaacgatttg taccgccgcg tcatcaaccg caacaaccgt
     8761 ctgaaacgcc tgctggaact gcatgcgcct gacatcatcg tccgcaacga aaaacgtatg
     8821 ttgcaagaag cggttgactc gctgttggat aacggccgtc gcggtaaagc catgaccgga
     8881 gccaacaaac gtccgctgaa atcgttggca gatatgatta aaggtaaagg cggccgcttc
     8941 cgtcaaaacc tgttgggcaa acgtgtggac tactccggcc gttccgtgat taccgtaggc
     9001 ccatacctgc gtctgcacca atgcggtttg ccgaaaaaaa tggcgttgga actgttcaaa
     9061 ccatttattt tccacaaatt ggaaaaacaa ggtttggcgt ctaccgttaa agctgcgaaa
     9121 aaattggtag aacaagaagt acctgaagta tgggatatct tggaagaagt catccgcgaa
     9181 catccgatta tgttgaaccg tgcgccgacc ctgcaccgtt tgggtattca agcgttcgag
     9241 cctattctga tcgaaggtaa agcgattcag ttgcacccat tggtgtgtgc cgcatttaat
     9301 gccgactttg acggtgacca aatggcagta cacgttccat tgagcttgga agcgcaaatg
     9361 gaagcacgca cgctgatgct ggcttcaaac aacgtattgt ctcctgccaa cggcgaaccg
     9421 attatcgtac cttcccaaga catcgtattg ggtctgtact acatgacccg cgaccgtatc
     9481 aatgccaaag gcgagggcag cctgtttgcc gatgtgaaag aagtgcatcg tgcataccat
     9541 accaaacagg tcgagctggg tacaaaaata accgtacgtc tgcgcgaatg ggtgaaaaac
     9601 gaagcaggtg agtttgagcc tgtcgttaac cgttacgaaa caaccgtcgg ccgtgcattg
     9661 ttgagcgaaa tcctgccgaa aggcctgccg tttgaatatg tcaacaaagc gttgaagaaa
     9721 aaagaaattt ccaagctgat taacgcatcg ttccgcctgt gcggcttgcg cgatacggtt
     9781 atcttcgccg accacctgat gtacaccggt ttcggatttg cggcaaaagg cggtatttcc
     9841 attgccgttg acgatatgga aattccaaaa gaaaaagcgg ccttgctggc tgaagccaat
     9901 gccgaagtta aagaaatcga agaccaatac cgtcaaggtt tggttaccaa cggcgaacgc
     9961 tacaacaaag tggtggatat ttggggtcgt gccggcgata agattgctaa agcgatgatg
    10021 gacaacctgt ccaaacaaaa agttatcgac cgtgacggca acgaagtcga tcaagagtca
    10081 ttcaactcca tttatatgat ggcggactcc ggtgcccgtg gttctgcggc tcagattaaa
    10141 cagttgtccg gtatgcgcgg tttgatggcg aaacctgacg gatcgattat tgaaacgccg
    10201 attacctcaa acttccgcga aggtctgacc gtattgcaat actttattgc gacccacggt
    10261 gcgcgtaagg gtttggcgga taccgcgttg aaaaccgcga actccggtta cctgacccgt
    10321 cgtctggtag acgtaactca agacttggtc gttgttgaag acgattgcgg tacttcagac
    10381 ggctttgtca tgaaggcagt ggtacaaggc ggtgatgtga ttgaagcatt gcgcgatcgt
    10441 attttgggtc gtgttaccgc gtctgacgtt gtcgatccgt caagcggcga gactttggtt
    10501 gaagccggta cgttgttgac tgaaaaactg gtggatatga ttgaccaatc cggtgtcgat
    10561 gaagtcaaag tccgtactcc gattacttgt aaaacccgcc acggtctgtg cgcgcactgt
    10621 tacggccgcg acttggcgcg cggcaaactg gtcaatgccg gcgaggcagt cggcgtgatt
    10681 gcggctcagt ctattggtga accgggtacc cagttgacca tgcgtacgtt ccacatcggt
    10741 ggtgcggcat cccgtgcggc agcagccagc caagtcgaag ccaaatccaa cggtacggca
    10801 cgtttcagca gccaaatgcg ttatgttgcc aacaacaaag gcgagttggt tgtcatcggc
    10861 cgttcttgcg aagtcgtgat tcacgatgac atcggccgtg aacgcgaacg ccacaaagta
    10921 ccttacggtg ccatcctgct ggtacaagac ggtatggcca ttaaagccgg tcaaaccttg
    10981 gcaacttggg atccgcatac ccgtccgatg attaccgaac atgcaggtat ggtgaaattc
    11041 gagaacatgg aagagggcgt taccgttgca aaacaaaccg atgatgtaac cggtttgtcc
    11101 gctttggtag tgattgacgg taaacgccgt tcctctagtg cttccaaact gctgcgtccg
    11161 actgtgaaac tcttggacga aaacggcgtg gaaatctgta ttcccggtac gtccactccg
    11221 gtttcgatgg cattccccgt tggtgcggtg attaccgtac gcgaaggtca ggaaatcggt
    11281 aaaggcgacg tattggcgcg tattccgcaa gcctcttcca aaacccgcga cattaccggc
    11341 ggtctgccgc gcgttgccga gctgtttgaa gcacgcgtgc cgaaagatgc cggtatgctg
    11401 gcggaaatta ccggtaccgt ttccttcggt aaggagacca aaggcaaaca acgtctgatt
    11461 atcactgacg tggacggtgt agcatacgag actttgattt ccaaagagaa acaaattctg
    11521 gtacatgacg gtcaagtggt aaaccgaggc gaaaccattg tggacggcgc ggtcgatccg
    11581 cacgatattc tgcgtctgca aggtatcgaa gcactggcac gctacattgt ccaagaggtg
    11641 caagaggttt accgcctgca aggtgtgaag atttccgata aacacatcga agtcatcatc
    11701 cgtcaaatgt tgcgccgtgt gaacattgcg gatgccggcg aaaccgggtt tattaccgga
    11761 gagcaggtcg aacgcggcga tatgatggcg gccaatgaaa aagctttgga agaaggcaaa
    11821 gagccggcac gttacgaaaa catattgctg ggtattacca aagcctccct gtccaccgac
    11881 agcttcattt ctgccgcatc gttccaagaa accacccgtg tgttgaccga agctgccatc
    11941 atgggcaaac aagacgaact gcgcggtctg aaagagaatg tcatcgtcgg tcgcctgatt
    12001 cctgccggta ccggtttgac ttatcaccgc agccgtcatc agcaatggca aggggtagag
    12061 caggagactg ctgaaaccca agtaacggat gaataatctt cggcgcgttc attcaataaa
    12121 aaaccgcaag ccttgagctt gcggtttttc tttgtccgat taaggcaaaa acaagcgttt
    12181 tcgtcatttt gaggcgtgtg gatataagcc cgataagtgt cccatatctt cgtgccgtca
    12241 aattatgcat cgaatagaac gagttaaatc cgaaggttat tgtgttaaat attgcaaaaa
    12301 tactttaaaa atttcgcacg gtgggagggt agaattccca gacttaaagc ttaagttttt
    12361 ttaacaaagt ttgggggagt ctaaatgaaa gtatctcaat cagcaatgaa taaacttgtt
    12421 attgctgtta ttgcttctat ggggctaaca gcgtgcgcgg gtggcggttc cgattcatct
    12481 atgtcggttc aaccaagcgt ttcggaacaa ctgaaagaca atgcaaatgt tgatgcgaaa
    12541 gatgaaaaag ttattgaata tctgaagaaa tcatcattaa aagatgtacc aaaagaattg
    12601 caagccaaag tattaaaggt taaaggtgat gaatatacag gagtgcgaaa acaatatgca
    12661 ggtaagctcg gtaagggtga atctgtcaag gcaatgctat ttctagacgg cgaagagccg
    12721 tttagtaagg aacaacttca gaagatggat gtatatgtta atggtaaaaa atacgaaggt
    12781 agtaaggggg ggaactagat gttctcccca aaggtttgag tgagcaaaag attgagtttt
    12841 acggtgcgga taaagaacag aattatgcgc tattaaaaac ttgggtttat gagcaaccgt
    12901 attctgtggt tagaggctat tttggctaca gtcgaaaaga cggtaatcct atagaagagg
    12961 atggacaaaa tcctgaagag attccttttg acttgtatct gggcgacatt agaggcgtgg
    13021 caacagatga agacaaatta cccaaggctg ggagctttca atatgaaggt cgggcatttg
    13081 gcggtaatgg ggttttatcc aaagaatcgt tagataacca taatggcgta tttagataca
    13141 caattgattt tgatcgtaga aaaggctctg gctcaattga aggaatggaa caatatggca
    13201 agatcaaatt agaagaagct gctattgaga gaatccctta tcgagagtcc ggctccagct
    13261 tggggttgaa agaccgggtt tcttatttcg gcgtgaacga aggggtggct atgttagaga
    13321 aggataatga gattaaaaag tatcatttgg gtattttcgg cgaagcggct aatgaagtag
    13381 caggtgctgt aagccaagag cataaacatc aagctgttat cggtttcggc ggcgagaaga
    13441 aatagagaaa gcactatttc tatttagtgc cgtttgtccg ggcaagatgc cgtctgaagt
    13501 ttcagacggc attttcttca aaaaacagtc cgccaaatcg aatactgcca agcggcaatg
    13561 caatacctca aaacagcaat aatttctttt aaaacagttg attcttactg aacataaatg
    13621 gccgggcaat acgaaaagaa aaatggcgtt gctgggcatt ttatttgaat ttgttatgaa
    13681 attgatgaaa tctaaaaata tcttttttat gggataccta atcgcttcgg cgacgtttgc
    13741 cgaggatatc ggcgtgcctg tcgaaccgat taacgtcggt agtcgggctg cgatgccgtc
    13801 tgaaggggaa agcctcgccc tcctgccgtt tgcagaggat gtgccgccgg ttcgcgatgc
    13861 aatgccgtct gaagttccta aaagcgcggc aggcggcgat gttcgaggcg gccgatctat
    13921 accgtgagtt gttgtcggaa agacccgatc ttgtttatcc ccgttttgat ttgggcgtga
    13981 tgctgtttga agacaaacaa tatcgtgagg cattggttca gttgcaccgg gccgaggagg
    14041 tgttgccgcc cgatatgcgg cagcttgcac gggaatatat ccgtcaggcg gaagcggtgc
    14101 aggcgtggca tccttcgttc aatatgaact atgaacaaac agataatgta aacaatgcgt
    14161 ccctttcacg ggacatcgtc atcaacggcc ggaaatggat aaaatcggaa gacagcctgc
    14221 ccaaacgcgc caacgggata aggtatgaat tgggtattga ccggatgttt aacatggcag
    14281 gcaatcactt tgcccgctta ggcatcagcg gttccggtgt gcattattgg aatgcccggg
    14341 attttagcga acaggcgttt catgccgaag taggctaccg ttatcggaat agccgtctag
    14401 agtggggatt ccggcccttt gtcaaacaaa accggctggg caataaccgt tatacggcaa
    14461 atacgggtat tgcgttggat tacagccgcc gtctgaatga aaaatggcgc agcacccaat
    14521 ctttccagta tggccgcaaa caataccatg acgaatattt ggcaaaacgt tacaacagta
    14581 aaacgatttc ggtgagcggt acgttcagct attacgctat gtcggcttgg cagttgtacg
    14641 ggggtattag cggtatgttt gataatactg tggagaaaga gcaggcttca cggcgttatg
    14701 gtgtaagttt gggtacggtg aaaatattag acggcggtct tggactgaag ttgggtgcgg
    14761 gttacacgaa acggatattt aaagcccctg caacattaat ttacaatttt actcggcgtg
    14821 atgattaaca tcggatgagc gcggctttat ggcataaaaa actgtcgtgg aaaggattta
    14881 caccccaaat aaatttccgt tacaacaaga acaacagcaa tatgcccgcc ttttattcgc
    14941 acagcggcaa gggatggttt gtcagtatgg aaaaaacgta ttgacagtat tttctccagc
    15001 cgtccgactg attgtgaggg atgtcggtaa atatttatcg gcaaacaaga aaatcatctt
    15061 ttttcttgtc gttatgcttg actgtctgct tgcaataaaa atataattcc actcttgccg
    15121 acatggtgtc ggcaagtatt taactcaaca ggacgagaaa atatgccaac tatcaaccaa
    15181 ttggtacgca aaggccgtca aaagcccgtg tacgtaaaca aagtgcccgc actggaagcc
    15241 tgcccgcaaa aacgcggcgt gtgcacccgt gtatacacga ctacccctaa aaaacctaac
    15301 tctgcattgc gtaaagtatg taaagtccgc ctgaccaacg gttttgaagt catttcatat
    15361 atcggcggtg aaggccacaa cctgcaagag cacagcgtcg tactgattcg cggcggccgt
    15421 gtaaaagact tgccgggtgt acgttaccac actgtacgcg gttctttgga tactgcaggt
    15481 gttaaagacc gcaaacaagc ccgttctaaa tacggtgcta agcgtcctaa ataattaccg
    15541 ggactcaaat aggcacgtcg gccgcctaag ctgaacaacg gccgagtaag tgaatactca
    15601 attgggtatt catgggaata gacccgactg aatagattaa aggaaattaa aatgccaaga
    15661 cgtagagaag tccccaagcg cgacgtactg ccagatccta aattcggtag cgtcgagttg
    15721 accaaattca tgaacgtatt gatgattgac ggtaaaaaat ccgttgccga gcgtatcgtt
    15781 tacggtgcgt tggaacagat tgagaaaaaa accggcaaag cagcaatcga agtatttaac
    15841 gaagccattg caaactccaa acctatcgtg gaagtgaaaa gccgccgtgt aggtggtgca
    15901 aactaccaag ttcctgttga agttcgtcct tcacgccgtc tggctttggc aatgcgttgg
    15961 gttcgcgacg cggcccgcaa acgtggtgag aaatccatgg atctgcgttt ggcaggcgag
    16021 ttgattgatg cgtccgaagg ccgtggcggt gcgttgaaaa aacgtgaaga agtacaccgt
    16081 atggctgaag ccaacaaagc attctctcac ttccgtttct aattttgaaa ggctaataaa
    16141 atggctcgta agaccccgat cagcctgtac cgcaacatcg gtatttccgc ccatatcgat
    16201 gcgggtaaaa ccacgacgac agaacgtatt ttgttctata ccggtttgac ccacaagctg
    16261 ggcgaagtgc atgacggtgc ggctactacc gactacatgg aacaagagca agagcgcggt
    16321 attaccatta cctccgctgc cgttacttcc tactggtccg gtatggcgaa acaattcccc
    16381 gagcaccgct tcaacatcat cgacaccccg gggcacgttg actttaccgt agaggtagag
    16441 cgttctatgc gtgtattgga cggcgcggta atggtttact gtgcggtggg cggtgttcaa
    16501 ccgcaatctg aaaccgtatg gcggcaagcc aacaaatacc aagttccgcg cttggcgttt
    16561 gtcaataaaa tggaccgcca aggtgccaac ttcttccgcg ttgtcgagca aatgaaaacc
    16621 cgtttgcgcg caaaccccgt acctatcgtc attccggtag gcgcggaaga cagtttcacc
    16681 ggtgttgtcg atttgctgaa aatgaaatct atcatctgga atgaagccga taaaggtaca
    16741 acctttacct atggcgatat tcctgccgaa ttggtcgaaa ctgccgaaga atggcgtcaa
    16801 aatatgattg aagccgcagc cgaagccagc gaagaactga tggacaaata cttgggcggt
    16861 gaagatctgg ccgaagaaga aatcgtaggc gcgttgcgtc aacgtacttt ggcaggcgaa
    16921 attcagccta tgctgtgcgg ttctgcattt aaaaacaaag gtgttcaacg tatgttggac
    16981 gcagttgtag aattgctgcc agctcctacc gatattcctc cggttcaagg tgttaatcct
    17041 aacactgaag aagccgacag ccgtcaagcc agcgatgaag agaaattctc tgcattggca
    17101 ttcaaaatgt tgaacgacaa atacgtcggt cagctgacct ttatccgcgt ttactcaggc
    17161 gtagtaaaat ccggcgatac cgtactgaat tctgtaaaag gcactcgcga acgtatcggt
    17221 cgtttggtgc aaatgactgc cgcagaccgt actgaaatcg aagaagtacg cgctggcgac
    17281 atcgcagccg ctatcggtct gaaagacgtt actaccggtg aaaccttgtg tgcggaaagc
    17341 gcgccgatta tcttggaacg tatggaattc cccgagccgg taatccatat tgccgttgag
    17401 ccgaaaacca aagccgacca agagaaaatg ggtatcgccc tgaaccgctt ggctaaagaa
    17461 gacccttctt tccgcgttcg tacagacgaa gaatccggtc aaaccattat ttccggtatg
    17521 ggtgagctgc acttggaaat tattgttgac cgtatgaaac gcgaattcgg tgtggaagca
    17581 aatatcggtg cacctcaagt ggcttaccgt gaaactatcc gcaaagccgt taaagctgaa
    17641 tacaaacatg caaaacaatc cggtggtaaa ggtcaatacg gtcacgttgt gattgaaatg
    17701 gaacctatgg aaccgggtgg tgaaggttac gagtttatcg atgaaattaa aggtggtgtg
    17761 attcctcgcg aatttattcc gtctgtcgat aaaggtatcc gcgatacgtt gcctaacggt
    17821 atcgttgccg gctatcctgt agttgacgta cgtatccgtc tggtattcgg ttcttaccat
    17881 gatgtcgact cttcccaatt ggcatttgaa ttggctgctt ctcaagcgtt taaagaaggt
    17941 atgcgtcaag catctcctgc cctgcttgag ccgattatgg cagttgaagt ggaaactccg
    18001 gaagaataca tgggcgacgt aatgggcgac ttgaaccgcc gtcgcggtgt tgtattgggt
    18061 atggatgatg acggtatcgg cggtaaaaaa gtccgtgccg aagtacctct ggcagaaatg
    18121 ttcggttact cgaccgacct gcgttctgca acccaaggcc gcgctactta ctctatggag
    18181 ttcaagaaat attctgaagc tcctgcccac atagctgctg ctgtaactga agcccgtaaa
    18241 ggctaatcag gcaaataggc cgtctgaaag gctgaaatga tttttcagat ggcattgttc
    18301 tttaatcgat ctttaatgta aaggaattag c
//
LOCUS       DAWTQS010000044        18272 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.044, whole genome shotgun sequence.
ACCESSION   DAWTQS010000044 DAWTQS010000000
VERSION     DAWTQS010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18272
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.044"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            275..973
                     /locus_tag="WNA25_001421"
     CDS             275..973
                     /locus_tag="WNA25_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFA9824154.1"
                     /translation="MKSRHLALGVAALFALAACDSKVQTSVPADSAPAASAAAAPAGL
                     VEGQNYTVLANPIPQQQAGKVEVLEFFGYFCPHCARLEPVLSKHAKSFKDDMYLRTEH
                     VVWQKEMLPLARLAAAVDMAAAESKDVANSHIFDAMVNQKIKLQEPEVLKKWLGEQTA
                     FDGKKVLAAYESPESQARAGKMQELTETFQIDGTPTVIVGGKYKVEFADWESGMNTID
                     LLADKVREEQKAAQ"
     gene            complement(1030..2034)
                     /locus_tag="WNA25_001422"
     CDS             complement(1030..2034)
                     /locus_tag="WNA25_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HFA9824155.1"
                     /translation="MQRQTELKNWLQTVYPERDFDLSFAAADADFRRYFRAAFSDGGS
                     VVCMDAPPDKMSVAPYLKVQKLFDMVNVPQVLHADTDLGFVVLNDLGNTTFLTAMLQE
                     QGEAAHKALLLEAIGELVGLQKASREGVLPEYDRETMLREINLFPEWFVAKELGRELT
                     FKQRQLWQQTADTLLPPLLAQPKVYVHRDFIVRNLMLTRGRPGVLDFQDALYGPISYD
                     LVSLLRDAFIEWEEEFVLDLVIRYWEKARAAGLPVPAEFDEFYRRFEWMGVQRHLKVA
                     GIFARLYYRDGKDKYRPEIPRFLNYLRRVSRRYAELAPLYALLVELVGDEELETGFTF
                     "
     gene            2104..4509
                     /locus_tag="WNA25_001423"
     CDS             2104..4509
                     /locus_tag="WNA25_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HFA9824156.1"
                     /translation="MARLFSLKPLVLALGFCFGTHCAADTVAAEEADGRVAEGGAQGA
                     SESAQAFDLTLGSTCLFCSNESGSPERTEAAVQGSGEASVPEDYTRIVADRMQGQSQV
                     KVRAEGSVIIERDGAVLNTDWADYDQSGDTVTVGDRFALQQDGTLIRGETLTYNLDQQ
                     TGEAHNVRMETEQGGRRLQSVSRTAEMLGEGRYKLTETQFNTCSAGDAGWYVKAASVE
                     ADRGKGIGVAKHAAFVFGGVPLFYTPWADFPLDGNRKSGLLVPSVSAGSDGVSLSVPY
                     YFNLAPNFDATFAPGIIGERGATFDGQIRYLRPDYSGQTDLTWLPHDKKSGRNNRYQA
                     KWQHRHDISDTLQAGVDFNQVSDSGYYRDFYGGEEIAGNVNLNRRVWLDYGGRAAGGS
                     LNAGLSVQKYQTLANQSGYKDEPYAIMPRLSADWHKNAGRAQIGVSAQFTRFSQDGRQ
                     DGSRLVVYPGIKWDFSNSWGYVRPKLGLHATYYSLDSFGGKASRSVGRVLPVVNIDGG
                     TTFERNTRLFGGGVVQTIEPRLFYNYIPAKSQNDLPNFDSSESSFGYGQLFRENLYYG
                     NDRINAANSLSTAVQSRILDGATGEERFRAGIGQKFYFKDDAVMLDGSVGKNPRSRSD
                     WVAFASGGIGGRFTLDSSIHYNQNDKRAEHYAVGAGYRPAPGKVLNARYKYGRNEKIY
                     LQADGSYFYDKLSQLDLSAQWPLTRNLSAVVRYNYGFEAKKPIEMLAGAEYKSSCGCW
                     GAGVYAQRYVTGENTYKNAVFFSLQLKDLSSVGRNPAGRMDVAVPGYIPAHSLSAGRN
                     KRP"
     gene            4611..5612
                     /locus_tag="WNA25_001424"
     CDS             4611..5612
                     /locus_tag="WNA25_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFA9824157.1"
                     /translation="MMKIKALMIAAALLAAADVHAAPQKAKTAPAKAVKAAATAQKEA
                     APAQQQGGIRFSDGIAAVADNEVITNRRLAEAVAEAKATLPEDAQISESELSRQVLMQ
                     LVNQSLIVQAGKRRNIQASEAEIDAVVAQNPALKNLSPTQRRELADNIIAEKVRQQAV
                     MQNSRVSEAEIDAFLEQAQKQGITLPEGAPLRQYRAQHILIKADSKNAAVGAESTIRK
                     IYDQARNGTDFAGLARRYSQDASAGNGGDLGWFADGVMVPAFEEAVHALKPGQVGAPV
                     RTQFGWHIIKLNEVRDAGTPEERIRNSVRQYIFQQKAGQATVNLLRDLHSGAYVDIR"
     gene            complement(6092..7972)
                     /locus_tag="WNA25_001425"
     CDS             complement(6092..7972)
                     /locus_tag="WNA25_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HFA9824158.1"
                     /translation="MNIFYEESGQFKVAVVVQKNDATYQVDTQHGKRTKVKANNVFAE
                     FDGDMAAFLENAQAQAADIDTDLLWEVCGEEEFTAEAIAEEYYGHAPTKTELAATLIA
                     LYAAPVYFYKKAKGVFKAAPEETLKQALAAIERKKQQDAQIDAWAEALKRGEMPSEIA
                     ADLRTILHAPDKQSLTYKAFTKAADALKTSAYELAKKTGGITSIPQYLQEGFEIKYFP
                     KGTGFPDLSLPEMPDLPKADVTAFSIDDESTTEVDDALSLTDLGNGTKRVGIHIAAPS
                     LAVRQGGGMEQIIMQRLSTVYFPGGKITMLPENWITAFSLDAGAYRPAVSIYFDVDGE
                     FNVGEPTCKIEAVNIAANLRIQAIEPHFNAETGLDQAGEMMFAHHQDLIWFYQFATAL
                     QKARGKYEPDRAPQYDYSIELDEEGNVSVVRRERGSPIDTLVSEMMILANSTWAQMLD
                     ENGLPGIFRVQPAGKVRMSTQSEPHIGMGVQHYGWFTSPLRRAADYINQKQLISLIDD
                     TAEPLYQKSDAALFAALRDFDTAYAAYADFQRQMEAYWSLVYLQQQGISELTATILKE
                     DLVRIEGLPLTTRATGIPFDALPKSQALFKITELDAEKQFVSLNYIKAAAPGGKTAGN
                     AV"
     gene            8128..8760
                     /locus_tag="WNA25_001426"
     CDS             8128..8760
                     /locus_tag="WNA25_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFA9824159.1"
                     /translation="MENVNRVPEQARYDAERRQADEALADVFPAVSIFGSARTPQDHA
                     DYAFACRLARRLSDSGIAVISGGGPGIMEAANKGAFAGKSVSVGLNIALPHEQKPNPY
                     QDIALRFSRFAERKAVFFRYSQAYVVMPGGFGTLDELFEILTLVQTGKVPPRPVVLVG
                     KAFWSGLAEWINAQLLARGMISEGAASLFSISDDEDEIIAYLSEHGLQTA"
     gene            8832..10202
                     /gene="purB"
                     /locus_tag="WNA25_001427"
     CDS             8832..10202
                     /gene="purB"
                     /locus_tag="WNA25_001427"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFA9824160.1"
                     /translation="MINPIASLSPLDGRYAQSVEALRPIFSEYGLMKARVKVELNWLK
                     ALAAAPEIAEVPPFSAETLAEIDKVIENFSLEDAAAVKAIEATTNHDVKAIEYWLKKR
                     FAEVPEVAAVSEFIHFACTSEDINNLSHALMLQEARETVILPKLAEIIGKLTAMAHDL
                     AAVPMMSRTHGQPATPTTLGKETANVVYRLQRQFKILQAQEFLGKINGAVGNYNAHMA
                     AYPDVDWETHCRNFVEISLGLTFNPYTIQIEPHDYMAEFFQTLSRVNTILIDFNRDVW
                     GYISLGYFKQKVKAGEVGSSTMPHKVNPIDFENSEGNLGMANAVLGFLSEKLPISRWQ
                     RDLTDSTVLRNMGVGVGYAVLGFAAHLRGLNKLEPNPAALAADLDVTWELLAEPIQTV
                     MRRYGVANPYEKLKDLTRGKGGITPEVLKGFIGSLEIPAEAKAKLLELTPALYVGKAE
                     ALAKRI"
     gene            10282..12147
                     /locus_tag="WNA25_001428"
     CDS             10282..12147
                     /locus_tag="WNA25_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFA9824161.1"
                     /translation="MQAVRYRPEIDGLRAVAVLSVIIFHLNNRWLPGGFLGVDIFFVI
                     SGFLITNIILSEIQNGSFSFRDFYTRRIKRIYPAFIAAVSLASVIASQIFLYEDFNQM
                     RKTIELSTVFLSNIYLGFRLGYFDLSADENPVLHIWSLAVEEQYYLLYPLLLIFCCKK
                     TKSLRALRHISIILFLILTATSFLPAAFYTDILNQPNTYYLSTLRFPELLVGSLLAVY
                     GQTQNGRRQTENGKRQLLSLLCFGALLVCLFVIDKHDPFIPGITLLLPCLLTALLIRS
                     MQYGTLPTRILSASPIVFVGKISYSLYLYHWIFISFAHYITGDKQLGLPAVSAVAALT
                     AGFSLLSYYLIEQPLRKRKMTFKKAFFCLYLAPSLMLVGYNLYSRGILKQEHLRPLPG
                     TPVAAENNFPETVLTLGDSHAGHLRGFLDYVGGREGWKAKILSLDSECLVWVDEKLAD
                     NPLCRKYRDEVEKAEAVFIAQFYDLRMGGQPVPRFEAQSFLIPGFQARFRETVKRIAA
                     VKPVYVFANNTSISRSPLREEKLKRFAINQYLRPIQAMGDIGKSNQAVFDLVKDIPNV
                     HWVDAQKYLPENTVEIHGRYLYGDQDHLTYFGSYYMGREFHKHESLLKHSRGGAL"
     gene            complement(12286..12900)
                     /locus_tag="WNA25_001429"
     CDS             complement(12286..12900)
                     /locus_tag="WNA25_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFA9824162.1"
                     /translation="MNFTRLLNQVLSTVQKKGSTFSGSPLNSFGGGALVAGVASMLLN
                     GKNRKTITKIGSTAALGYLAYRGYQMWQQNKGRATVTQSDFQPAGKTEETYSRTVLRT
                     MIAAAASDGMIDEAERRTIEQESGTDPETAAWLAAEYRLPASIGDIAAAVGNDEALAA
                     ETYLAARLVCADLSRKETVFLARLSQALELDDNLVESLERQLGI"
     gene            complement(12936..15086)
                     /gene="dinG"
                     /locus_tag="WNA25_001430"
     CDS             complement(12936..15086)
                     /gene="dinG"
                     /locus_tag="WNA25_001430"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFA9824163.1"
                     /translation="MLTDLEKNAIRDHYQNIGKNLPGFRPRASQREMIAAVANAFSRT
                     LTREEGGEPPKREGESIAVIEGPTGVGKSLAYLLAGGIMAQTRGKRLIVSSATVALQE
                     QLVDRDLPFLVEKSGLELTFALAKGRGRYLCPYKLYQLTQSNAQQNLLGFEAPAVLWD
                     SKPKPEELKLLRDIADEFSARRFNGDRDAWPEKIDDAIWLKVTNDRHGCLKAACPNRP
                     ECPFYLARDMLETVDVVVANHDLLLADISMGGGVILPAPENSFYCIDEAHHLPKKALS
                     RFAAEHSWNIAVWTLEKLPQLTGKIAALTDKAELANLADEAAASLLDSLHEWQFHLAE
                     EPSLSMGLSENDRRTNSEPTWLWEDGKIPEGLETTVSNTAIAARSLLKHVVGLNDALS
                     AARREKEQDGALIDRLTGEFGLFIARIEQISAVWDLLSTVSIEGEEPLAKWIARRADD
                     KNDYIFNASPISSASHLANSLWRRAAGAVLTSATLQSLGSFNLILRQTGLLWLPETTT
                     LALKSPFDFEKQGELYIPSIYASPKDPEAHTAAVIEWLPKLISPTEAIGTLVLFSSRK
                     QMQDVALHLPGDYLPLLLVQGELPKAVLLQKHHQAIEEGKASIIFGLDSFAEGLDLPG
                     TACVQVIIAKLPFAMPDNPIEKTQNRWIEQRGGNPFIEITVPEAGIKLIQAVGRLIRT
                     EQDYGRVTILDNRIKTQRYGQQLLAGLPPFKRIG"
     gene            complement(15269..16567)
                     /locus_tag="WNA25_001431"
     CDS             complement(15269..16567)
                     /locus_tag="WNA25_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFA9824164.1"
                     /translation="MPSETALLLYADTRAAHTLVWFRQNLRIRDNAALCAAVAEGSPV
                     IGIWIDDAETDNPRRAAFYRQSAAELAQGLARRGIPLYTAASPAGLVRLAVRLNIRAV
                     IADESHTFADKLADNALWHELDKHGIALTFVNDRSVFGKTDLTPDNGTAHTDFNRYRE
                     VWLDRFSKQPPAGSDLFAAYRQPFPENLPAPPPAALSDGIFLPQNGGETAAWRQWRRF
                     LEQAASYSVLKDFPSRKNTSLMGAYLSVGCISPRLLARESLERRLNAWADNIIRRDFF
                     LQLALQHADDDPSDGNPEHTLRLTLWQQGRTGIPIIDAAMRCLHKTGSLHPALRRLSA
                     DFFCHVLNLPRREGEIWFARQLTDFDAAINQGNWRLAASRHTCPDIAAASYRTDPDGT
                     FIKRHIPELAHLSADTVHTPWRFACSVDTHGYPARPVAGV"
     gene            complement(16557..17468)
                     /locus_tag="WNA25_001432"
     CDS             complement(16557..17468)
                     /locus_tag="WNA25_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFA9824165.1"
                     /translation="MDTLFSLKVFRQVVQSGGFTRAADALGISTAMASKHVSHLENTV
                     QAKLLHHNSRNLSLTEAGEEYYRQCSYALDTLDDAAQKAAGGTEKPQGLLRVTMPLWF
                     AGSQICNWLAEYRERYPEVALELILDNRHVDLIAEGVDLALRVSQTLSPSLIARPLAE
                     IEFALLASPDFLRRNGVPETPEEVAGLPAVLPTYTNQQKLDLTRKSDGKKYRLELTPV
                     IRTDNTLMMREMIKAGACIGYQPLWAAEHDLRCGPLVRLLPGYAVPTDRLNAVYADRA
                     FLSAKVRSFIDFLNEKIASRKGCRNAV"
     gene            17576..17974
                     /locus_tag="WNA25_001433"
     CDS             17576..17974
                     /locus_tag="WNA25_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFA9824166.1"
                     /translation="MSDCCNRIQPVLLSVLRIVTAYLFLLHGTSKIFAFPIEMGSGSP
                     GGLLLLAGILEIVGGILLVLGLFARPAAFVLSGQMAVAYFMAHASGNALFPIANGGES
                     AVLFCFVFLYIAAAGGGAWSLDRLFFKRKA"
     gene            18023..18211
                     /locus_tag="WNA25_001434"
                     /pseudo
     CDS             18023..18211
                     /locus_tag="WNA25_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201279.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
BASE COUNT         3920 a         4997 c         5248 g         4107 t
ORIGIN      
        1 aaggggtgta acccttccaa atcagggcaa cgcatagggc ggtgctttat gtgccgtcct
       61 gtgtgttgaa acatcattgt tccccaacag ccgtatccgt ccggggcggc gcgtattgta
      121 cgacagaaac cgcttcggat ggcatcgggt ttgatgccgt ctgaagcggt ttcccgaaac
      181 gaaaacgtcc tttttcggcg gcaaactgta tcaacacgcg aaaatacaat aaaataccaa
      241 tatccgattt atttccaaca tcacggagag ccgtatgaaa tccagacacc tcgccctcgg
      301 cgttgccgcc ctgttcgccc ttgccgcgtg cgacagcaaa gtccaaacca gcgtccccgc
      361 cgacagcgcg cctgccgctt cggcagccgc cgccccggca ggactggtcg aagggcaaaa
      421 ctacaccgtc cttgccaacc cgattcccca acagcaggca ggcaaggttg aagtgcttga
      481 gtttttcggc tatttttgtc cgcactgcgc ccgcctcgaa cctgttttga gcaaacacgc
      541 caagtctttt aaagacgata tgtacctgcg taccgaacac gtcgtctggc agaaagaaat
      601 gctgccgctg gcacgcctcg ccgccgccgt cgatatggct gccgccgaaa gcaaagatgt
      661 ggcgaacagc catattttcg atgcgatggt caaccaaaaa atcaagctgc aagagccgga
      721 agtcctcaaa aaatggctgg gcgaacaaac cgcctttgac ggcaaaaaag tccttgccgc
      781 ctacgaatcc cccgaaagtc aggcgcgcgc cggcaaaatg caggagctga ccgaaacctt
      841 ccaaatcgac ggtacgccca cggttatcgt cggcggcaaa tataaagttg aatttgccga
      901 ctgggagtcc ggtatgaaca ccatcgacct tttggcggac aaagtacgtg aagaacaaaa
      961 agccgcgcag taggcccgtt tgaaaaatgc cgtctgaaac ttggttttca gacggcattt
     1021 tgattgggtt tagaacgtaa agcccgtttc cagttcttca tcgccgacca gttcgaccaa
     1081 gagcgcgtag agcggggcga gttcggcata acggcgcgat acgcggcgca gatagtttaa
     1141 gaaacgcggg atttccggac ggtatttgtc tttgccgtcg cggtagtaca ggcgtgcgaa
     1201 gatgcccgcg accttcaagt gccgctgcac gcccatccat tcgaaccggc ggtaaaactc
     1261 gtcaaactcg gcagggacag gcaagccggc agcccgcgcc ttttcccagt agcggataac
     1321 caaatccaaa acaaactctt cttcccactc gataaaggca tcgcgcaaca gcgacaccaa
     1381 atcgtaggaa atcgggccgt aaagcgcgtc ttggaagtct aaaacgcccg gcctgccgcg
     1441 cgtcagcatc aggttgcgga cgataaagtc gcggtgcacg tagactttgg gctgcgccaa
     1501 caagggcggc agcagcgtat cggcggtttg ctgccaaagt tggcgttgtt tgaatgttaa
     1561 ttcgcgcccc aattcttttg cgacaaacca ttccgggaac aggttgattt cgcgcagcat
     1621 cgtttcacgg tcatattcgg gcaaaacccc ttcacggctt gccttctgca atccgaccag
     1681 ctcgccgatg gcctccagca acagggcttt gtgtgccgct tcgccctgct cctgaagcat
     1741 cgcggtcaaa aacgtcgtat tgcccaagtc gttcaatacc acaaacccca aatccgtgtc
     1801 cgcgtgcaat acctgcggca cattgaccat gtcaaacagc ttctgcacct tcaaataagg
     1861 cgcgacactc atcttgtcgg gcggcgcatc catacagacg acgctgccgc cgtctgaaaa
     1921 cgccgcacgg aaatagcggc ggaaatcagc atccgccgcc gcgaaggaca gatcgaagtc
     1981 ccgttcggga taaacggtct gaagccaatt tttcagttcg gtttgccgtt gcataacagt
     2041 atccgggcat ttcgggttac aataaacact attctaaccg gtaaaccgac ttgaggggcg
     2101 attttggctc gtttattttc actcaaacca ctggtgctgg cattgggctt ctgtttcggc
     2161 acgcattgtg ccgccgatac cgttgcggcg gaagaggcgg acgggcgtgt cgcagaaggc
     2221 ggtgcgcagg gcgcgtccga atccgcacaa gctttcgatt tgaccctcgg ttcgacctgc
     2281 ctgttttgca gtaacgaaag cggcagcccc gagagaaccg aagccgccgt ccaaggcagc
     2341 ggcgaagcat ccgtccccga agactatacg cgcattgttg ccgacaggat gcaaggacag
     2401 tcgcaggtta aggtgcgcgc ggaaggaagc gttatcatcg aacgggacgg cgcagtcctc
     2461 aataccgatt gggcggatta cgaccagtcg ggcgacaccg ttaccgtagg cgaccggttc
     2521 gccctccaac aggacggtac gctgattcgg ggcgaaaccc tgacctacaa tctcgatcag
     2581 cagaccggcg aagcgcacaa cgtccgtatg gaaaccgaac aaggcggacg gcggctgcaa
     2641 agcgtcagcc gcaccgccga aatgttgggc gaagggcgtt acaaactgac ggaaacccaa
     2701 ttcaacacct gttccgccgg agatgccggc tggtatgtca aggccgcctc tgtcgaagcc
     2761 gatcggggaa aaggcatagg cgttgccaaa cacgccgcct tcgtgttcgg cggcgttccc
     2821 cttttctata cgccttgggc ggacttcccg cttgacggca accgcaaaag cggactgctc
     2881 gtcccgtccg tatctgccgg ttcggacggc gtttcccttt ccgtccccta ttatttcaac
     2941 cttgccccca acttcgatgc cactttcgcc cccggcatta tcggcgaacg cggcgcgacg
     3001 tttgacggac aaatccgtta cctgcgtccc gattacagcg gacagaccga cctgacctgg
     3061 ctgccgcacg ataagaaaag cggcaggaac aaccgctatc aggcaaaatg gcagcaccgg
     3121 cacgacattt ccgacacgct tcaggcgggt gtcgatttca accaagtctc cgacagcggc
     3181 tactaccgcg acttttacgg cggcgaagaa atcgccggca acgtcaacct caaccgccgc
     3241 gtatggctgg attatggcgg cagggcggcg ggaggcagcc tgaatgccgg cctttcggtt
     3301 cagaaatacc agacgctggc aaaccaaagc ggctacaaag acgaacctta cgccatcatg
     3361 ccccgccttt ctgccgattg gcataaaaac gcaggcaggg cgcaaatcgg cgtgtccgca
     3421 caatttacac gcttcagcca agacggccgc caagacggca gccgactggt cgtgtatccc
     3481 ggtatcaaat gggatttcag caacagctgg ggctacgtcc gccccaaact ggggctgcac
     3541 gccacttatt acagcctcga cagtttcggc ggcaaagcat cccgcagcgt cgggcgcgtt
     3601 ttgcccgttg tcaatatcga cggcggcaca accttcgaac gcaatacgcg cctgttcggc
     3661 ggcggagtcg tgcaaaccat cgagccgcgc ctgttctaca actatattcc tgccaaatct
     3721 caaaacgacc tgcccaattt cgattcgtcg gaaagcagct tcggctacgg gcagcttttc
     3781 cgcgaaaacc tttattacgg caacgaccgc atcaacgccg ccaacagcct ttccaccgcc
     3841 gtgcagagcc gtattttgga cggcgcgacg ggggaggagc gtttccgcgc cggtatcggt
     3901 cagaaattct atttcaagga tgatgcggtg atgcttgacg gcagcgtcgg caaaaatccg
     3961 cgcagccgtt ccgactgggt ggcattcgcc tccggcggca taggcgggcg tttcaccctc
     4021 gacagcagca tccactacaa ccaaaacgac aaacgcgccg aacattacgc cgtcggcgca
     4081 ggctaccgcc ccgcccccgg aaaagtgttg aacgcccgct acaaatacgg gcgcaacgaa
     4141 aaaatctacc tgcaggcgga cggttcctat ttttacgaca aactcagcca gctcgacctg
     4201 tccgcacaat ggccgctgac gcgcaacctg tctgccgtcg tccgctacaa ctacggtttt
     4261 gaagccaaaa aaccgataga aatgcttgcc ggtgcagaat acaaaagcag ttgcggctgc
     4321 tggggcgcgg gcgtgtacgc ccaacgctac gttaccggcg aaaacaccta caaaaacgcc
     4381 gtcttttttt cacttcagtt gaaagacctc agcagcgtcg gcagaaaccc cgcaggcagg
     4441 atggatgtcg ccgttcccgg ctacatcccc gcccactctc tttccgccgg acgcaacaaa
     4501 cggccctgac cgtcggaaac ccggcaggag caccgttcct gcacaagata gcattccacc
     4561 gacaacccca aacccgccat caaaggcagg attcaaacga taaggaaaga atgatgaaaa
     4621 tcaaagccct gatgattgcc gccgcattgc tggcagcagc cgatgtccac gccgcaccgc
     4681 aaaaggcaaa aaccgcgccc gccaaagcgg tcaaagctgc cgccacggcg caaaaagaag
     4741 ccgcacccgc acaacagcag ggcggtatcc gcttttcaga cggcattgcc gccgttgccg
     4801 acaacgaagt catcaccaac cgccggcttg ccgaagccgt tgccgaagcc aaagccaccc
     4861 tgcccgaaga cgcgcagata agtgaatccg agctgtcccg acaggtgctg atgcagcttg
     4921 tcaaccaatc cctgattgta caggcgggca aacgccgcaa cattcaggca agtgaagcgg
     4981 aaatcgatgc cgtcgtcgca caaaatcccg ccctcaaaaa cctcagcccc acccaacgcc
     5041 gcgagcttgc cgacaacatc attgccgaaa aagtccgcca gcaggcagtg atgcaaaaca
     5101 gccgcgtgag cgaagctgaa attgatgcct tcctcgagca ggcgcaaaaa caaggcatca
     5161 ccctgcccga aggcgcaccg ttgcgccaat accgcgccca acacatcctg attaaagccg
     5221 acagcaaaaa cgccgccgtc ggcgcggaaa gtaccatccg caaaatctac gaccaggcgc
     5281 gcaacggcac ggatttcgca ggtttggcac gccgatattc ccaagacgca agcgcgggca
     5341 atggcggcga cttgggctgg tttgccgacg gcgtaatggt tcccgccttt gaagaagccg
     5401 tccacgcgct caaacccgga caggtcggcg cgcctgtccg cacccaattc ggctggcaca
     5461 tcatcaaatt gaacgaagtg cgcgatgccg gcacgccgga ggaacgtatc cgcaattccg
     5521 tgcggcaata catcttccaa caaaaagccg gacaggcaac cgtcaacctg ttgcgcgacc
     5581 tgcattccgg cgcgtatgtc gacatccgct aaggcggttt gaatccaaaa gtcataccga
     5641 tcgggcaaaa gtccgggcgg tatggctttt ttgaatttcg agttactttt acaccgccat
     5701 tcgtcattcc cgcgcaggcg ggaatctagc aacgaaaagt aacaggaatt tatcgggaat
     5761 ggccggagtt taaaggtctg gattcccgcc ttcgcgggaa tgacgattca gaagttacac
     5821 gaaactcaaa aaaaaaaacg aaaccgaacg aaccggattc ccgcttttac gggaatgacg
     5881 gcatatcggt ttccgtgagg acaaacctag attcccgcct gcgcgggaat gacgggattt
     5941 tagattgcgg gtatttatcg ggaatggcga tttaaaaatt acctaaaact aaacttaaaa
     6001 gcaactgaaa cttaaaaaac taaaatttcg cctgcgtggg aaggatggga tttcagattt
     6061 ctgtttttgt gggaatttgc cgtatcgggc ttcagacggc attgcctgcc gttttgcccc
     6121 cgggtgcggc tgccttgatg tagttgagcg agacaaactg cttctcggca tccaattcgg
     6181 tgattttaaa caatgcctgc gatttgggca gcgcgtcaaa cggaataccg gtcgcgcgcg
     6241 ttgtcagtgg cagaccttcg atgcggacga ggtcttcttt cagaatcgtc gcggtcagtt
     6301 cgctgatgcc ttgttgttgc aggtacacga ggctccagta ggcttccatc tgccgttgga
     6361 agtcggcgta ggcggcgtag gcggtgtcga aatcgcgcag ggcggcgaag agcgcggcat
     6421 cgcttttttg atacagcggc tcggcagtgt cgtcgatcag gctgatcagc tgcttttggt
     6481 tgatgtagtc ggcggcgcgg cgcagcggcg aggtaaacca gccgtaatgc tgcacgccca
     6541 tgccgatgtg cggctcggat tgggtgctca tgcgcacttt gcctgccggt tggacgcgga
     6601 aaatgccggg cagcccgttt tcgtctagca tttgcgccca agtgctgttg gcaagaatca
     6661 tcatctcgct gaccagcgta tcgatgggcg aaccgcgttc gcggcggacg acggagacgt
     6721 tgccttcttc atccaattcg atgctgtaat cgtattgcgg cgcgcggtcg ggttcgtatt
     6781 tgccgcgcgc tttttgcagg gcggtggcga attgatagaa ccaaatcagg tcttgatggt
     6841 gggcgaacat catttcgcct gcttggtcca agccggtttc tgcgttgaaa tgcggctcga
     6901 tggcttggat acgcaggttt gcggcgatgt tgacggcttc gattttgcag gtcggctcgc
     6961 cgacgttgaa ctcgccgtcc acatcgaaat aaatgctgac ggcagggcgg tatgcgcctg
     7021 catcgaggct gaacgcggta atccagtttt cgggcagcat cgtgattttg ccgccgggga
     7081 aataaaccgt actcaaccgc tgcataatga tttgttccat tccgccgcct tgtcggacgg
     7141 caagcgacgg cgcggcgatg tggatgccga cgcgcttcgt gccgttgccc aaatcggtca
     7201 ggctcaaggc atcgtccact tcggtggtcg attcgtcgtc aatggaaaag gcggtaacgt
     7261 cggccttggg caggtcgggc atttcgggaa gggagaggtc ggggaaacct gttcctttgg
     7321 ggaagtattt gatttcaaag ccttcttgca ggtattgcgg aatggacgta atgccgcccg
     7381 tttttttcgc cagttcgtag gcggaggttt tcagtgcgtc ggcggctttg gtaaaggctt
     7441 tgtaggtcag cgactgtttg tcgggcgcgt gtaagatggt tctcaaatcc gccgcgattt
     7501 cagacggcat ctcgccgcgt ttcaaggctt ccgcccaagc gtcgatttgc gcgtcttgct
     7561 gttttttgcg ttcgatggcg gcaagcgctt gtttcaaagt ttcttcgggc gcggctttga
     7621 acacgccttt ggcttttttg tagaaataca ccggcgcggc gtacagcgca atcaaagtcg
     7681 ccgccagctc ggttttggtc ggcgcatggc cgtaatattc ttcggcgatg gcttcggcgg
     7741 taaactcctc ttcgccgcat acttcccaca ataaatcggt gtcgatgtcc gccgcctgcg
     7801 cctgagcgtt ttccaaaaac gccgccatat cgccgtcaaa ctcggcaaag acattgtttg
     7861 ccttcacttt ggtgcgtttg ccgtgttggg tatcgacttg gtaggtagcg tcgttttttt
     7921 ggacgacgac ggcaactttg aactggccgg actcttcgta aaaaatattc atttttcgga
     7981 tttttctgtg gaaactcaag cgggcgattt tagcagatta ccgaaaatgc cgtctgaaaa
     8041 aaggttgggg gaaggcgggt gcggctttgc ggtgcttgcg ttgcgttttt gacggggtac
     8101 aatcgccgtt tttgaacgga ggacggaatg gagaatgtaa accgcgtgcc ggagcaggca
     8161 cgttatgatg ccgaacgcag gcaggcagac gaagcattgg cggacgtgtt tccggcagtc
     8221 agtattttcg gcagcgcgcg cacgccgcag gatcatgcgg attatgcgtt tgcctgccgt
     8281 ctggcgcggc ggctgtcgga ttcgggtatt gccgtcattt cgggcggcgg gccggggatt
     8341 atggaggcgg caaacaaggg cgcgtttgcc gggaagtcgg tttcggtagg gctgaacatt
     8401 gccctgccgc acgagcagaa accgaatccg tatcaggaca tcgccttgcg gttttcccgt
     8461 tttgccgaac gcaaggcagt gtttttccgc tattcccaag catatgtcgt gatgccgggc
     8521 ggcttcggga cgctggacga attgtttgaa atcctgacct tggtgcagac gggcaaagtg
     8581 ccgccgcgtc cggtcgtttt ggttggaaag gcgttttggt cgggcttggc ggagtggata
     8641 aacgcgcagc ttttggcgcg cggtatgatt tccgaagggg ccgcttcttt gttctccata
     8701 tcggacgacg aagacgaaat cattgcgtat ctgtcggaac acgggcttca gacggcatag
     8761 cgtcctgaga gtgatgtata attgcaaaca atttgtcaat ttttgatgtc ttttccgaac
     8821 aggatgccga tatgatcaac cccatcgcct cgctttcccc cctagacggc cgttatgccc
     8881 aatccgttga agcattgcgc ccgatttttt cagagtacgg actgatgaag gcgcgcgtca
     8941 aagtcgaatt aaactggctc aaagccctgg ccgccgcgcc ggagattgcc gaagtgccgc
     9001 ccttcagtgc cgaaacgctt gccgaaatcg acaaagtcat cgaaaacttt tcattagaag
     9061 acgcggccgc cgtcaaagcc atcgaagcca cgaccaatca tgatgtcaaa gccatcgaat
     9121 attggctgaa aaaacgtttt gccgaagtgc cggaagtcgc cgccgtgagc gagttcatcc
     9181 acttcgcctg caccagcgaa gacatcaaca acctgtccca cgccctgatg ctgcaagagg
     9241 cgcgcgaaac cgtcatcctg cccaaacttg ccgaaatcat cggcaaactc accgctatgg
     9301 cgcacgacct tgccgccgtc ccgatgatga gccgcaccca cggccagccc gccacaccga
     9361 ccactttggg caaagaaacc gccaatgtcg tgtaccgcct gcaacgccag ttcaaaatcc
     9421 ttcaggcgca agaattcctc ggcaaaatca acggcgcggt cggcaactac aacgcccaca
     9481 tggccgccta tcccgatgtg gattgggaaa cacactgccg caacttcgtc gaaatcagcc
     9541 tcggcctgac cttcaacccc tacaccatcc aaatcgagcc gcacgactat atggcggagt
     9601 ttttccaaac cctcagccgt gtcaacacca tcctgatcga ctttaaccgc gacgtttggg
     9661 gttatatttc attgggttat ttcaaacaaa aagtcaaagc gggcgaagtc ggttcttcca
     9721 ccatgccgca caaagtcaac cccatcgact ttgaaaactc cgagggcaac ctcggcatgg
     9781 caaacgccgt attgggcttt ttgtccgaaa aactgccgat ttcccgctgg cagcgcgacc
     9841 tgaccgacag caccgtattg cgcaatatgg gcgtgggcgt gggctatgcc gtgttgggtt
     9901 tcgccgccca cctgcgcggt ctgaacaagc tcgagccgaa ccctgccgcg cttgccgccg
     9961 atttggatgt cacttgggag ctgctcgccg agccgattca aaccgtaatg cgccgttacg
    10021 gtgtcgccaa tccttacgaa aaactgaaag acctgacgcg cggtaaaggc ggcatcacac
    10081 ccgaagtgct gaaaggcttt atcggatcgc tggaaattcc cgccgaagcc aaagccaaat
    10141 tgcttgagct gacccccgcg ctgtatgtgg gcaaggctga agcgttggcg aaacggattt
    10201 gagcgtttac tgaaaccgat gccgtccgaa cgcgcgttca gacggcattt ttaagataac
    10261 gggacatacg gggcgatatt gatgcaagct gtccgataca ggcctgaaat tgacggattg
    10321 cgggccgtcg ccgtgctatc cgtcattatt ttccacctga ataaccgctg gctgcccgga
    10381 ggattcctgg gggtggacat tttctttgtc atctcgggat tcctcattac caacatcatt
    10441 ctttctgaaa tacagaacgg ttctttttct ttccgggatt tttatacccg caggattaag
    10501 cggatttatc ctgcttttat tgcggccgtg tccctggctt cggtgattgc ttctcaaatc
    10561 ttcctttacg aagatttcaa ccaaatgagg aaaaccatag agctttctac ggtttttttg
    10621 tccaatattt atttggggtt ccgattgggg tatttcgatt tgagtgccga cgagaacccc
    10681 gtactgcata tctggtcttt ggcggtagag gaacagtatt acctcctgta tcctcttttg
    10741 ctgatattct gctgcaaaaa gacaaaatcg ctacgggcgt tgcgccacat cagcatcatc
    10801 ctgttcctga ttttgactgc cacatcgttt ttgccggccg cgttttatac cgacatcctc
    10861 aaccaaccca atacttatta cctttcgaca ctgaggtttc ccgagctgtt ggtgggttcg
    10921 ctgttggcgg tttacgggca aacgcaaaac ggcagacggc aaacagaaaa tggaaaacgg
    10981 cagttgcttt cattactctg tttcggcgca ttgcttgtct gcctgttcgt gatcgacaaa
    11041 cacgatccgt ttatcccggg aataaccctg ctccttccct gcctgctgac ggcgctgctt
    11101 atccggagta tgcaatacgg gacacttccg acccgcatcc tgtcggcaag ccccatcgta
    11161 tttgtcggca aaatctctta ttccctatac ctgtaccatt ggatttttat ttccttcgcc
    11221 cattacatta caggcgacaa acagctcgga ttgcctgccg tatcggcggt tgccgcgttg
    11281 acggccggat tttccctgtt gagctattat ttgattgaac agccgcttag aaaacggaag
    11341 atgaccttca aaaaggcatt tttctgcctt tatctcgccc cgtccctgat gcttgtcggt
    11401 tacaacctgt attcaagagg gatattgaaa caggaacacc tccgcccgct gcccggcacg
    11461 cccgttgctg cggaaaataa ttttccggaa accgtcttga ccctcggcga ctcgcacgcc
    11521 ggacacctgc ggggttttct ggattatgtc ggcggcaggg aagggtggaa agctaaaatc
    11581 ctgtccctcg attcggagtg tttggtttgg gtggatgaga agctggcaga caacccgttg
    11641 tgccgaaaat accgggatga agttgaaaaa gccgaagccg ttttcattgc ccaattctat
    11701 gatttgagga tgggcggcca gcccgtgccg agatttgaag cgcaatcctt cctgataccc
    11761 ggattccaag cccgattcag ggaaaccgtc aaaaggatag ccgccgtcaa acccgtctat
    11821 gtttttgcaa acaacacatc aatcagccgt tcgcccctga gggaggaaaa attgaaaaga
    11881 tttgctataa accaatatct gcgccccatt caggctatgg gcgacatcgg caagagcaat
    11941 caggcggtct ttgatttggt taaagatatt cccaatgtgc attgggtgga cgcacaaaaa
    12001 tacctgcctg aaaacacggt cgaaatacac ggacgctatc tttacggcga ccaagaccac
    12061 ctgacctatt tcggttctta ttatatgggg cgggaattcc acaaacacga aagcctgctc
    12121 aagcattccc gaggcggcgc attgtagtag cctgccttct tgccggatat tgcctttggc
    12181 agcctatgcc gctgtttgcc ctttgaggcg gcttttattt tgccgttttg aggccggggt
    12241 cgggaataac cgttttttga tgattttccc tccccggctg tgtcatcaaa tccctagttg
    12301 cctttccaaa ctctccacca gattgtcatc cagttccaaa gcctgcgaca ggcgggcgag
    12361 gaagacggtt tctttccgcg acaaatcggc acagaccaac cttgccgcca gataggtctc
    12421 cgccgccaac gcctcatcgt tgccgacggc ggcggcgatg tccccgatgc ttgcgggcag
    12481 gcggtattcg gcggcgagcc atgcggcagt ttcggggtct gtgccgcttt cctgttcgat
    12541 agtccggcgt tcggcttcgt ctatcatgcc gtctgaagcg gcggcggcta tcatggtacg
    12601 caatacggtg cggctgtatg tttcttcggt ttttccggca ggttggaaat cgctttgtgt
    12661 tacggttgcc cgccctttgt tttgctgcca catctgatag ccccggtagg cgaggtagcc
    12721 caaagcggcg gtcgaaccga ttttggtgat ggttttgcgg tttttaccgt tcagcagcat
    12781 ggaggcgaca ccggcaacca gcgcgcctcc gccgaatgaa ttgagcgggc tgccggagaa
    12841 tgtgctgcct tttttttgaa ccgtgcttaa gacttggttg agcaaccggg tgaagttcat
    12901 gatttttcct ttctgttgcg ggaagggcgg tatgtttacc ctatcctttt aaacggtggc
    12961 aggccggcca ataattgttg gccgtaccgc tgcgttttga tgcggttgtc gaggatggtt
    13021 acgcggccgt agtcttgttc ggtgcggatg aggcggccga cggcctggat gagtttgatg
    13081 ccggcttcgg ggacggtgat ttcgatgaag gggttgccgc cgcgttgttc tatccaacgg
    13141 ttttgggttt tttcgatggg gttgtcgggc atggcgaagg ggagtttggc gatgatgact
    13201 tgcacgcagg cggtgccggg caggtcgagc ccttcggcga agctgtcgag tccgaagatg
    13261 atgctggctt tgccttcttc tatggcttgg tggtgttttt gcaggaggac ggctttgggt
    13321 aattcgcctt gtacgagcaa gagcggcagg tagtctccgg gcaggtgcag ggcgacatcc
    13381 tgcatttgtt tgcgcgagga aaacaggacg agtgtgccga tggcttcggt gggcgaaatc
    13441 agcttgggca gccattcgat gacggcggcg gtgtgggctt cggggtcttt ggggctggcg
    13501 tatatggagg ggatgtagag ttcgccctgt ttttcaaagt caaaggggct tttgagggcg
    13561 agggtggtgg tttcgggcag ccacagcagg ccggtttggc gcaaaatcaa gttgaagctg
    13621 ccgagcgatt gcagggtggc cgaagtcagc accgcgcctg ccgcgcgccg ccacaggctg
    13681 ttggcaaggt gggacgcgct gctgatgggg ctggcattga aaatatagtc gtttttgtcg
    13741 tcggcacggc gggctatcca tttcgccaac ggctcctcgc cctcaatgga aacagtggag
    13801 agcaaatccc aaaccgcgct gatttgttcg atacgggcga taaaaaggcc gaactcgccg
    13861 gtcaggcggt cgatgagcgc accgtcctgt tctttttcgc ggcgtgcggc ggaaagcgca
    13921 tcgttcagcc cgacaacgtg tttgagcagg ctgcgtgcag caatggccgt attggaaacg
    13981 gtggtttcga ggccttcggg gattttgccg tcttcccaca gccaagtcgg ttcgctgttg
    14041 gttcgtctgt cgttttcaga cagccccata cttaaagacg gctcttccgc caaatggaat
    14101 tgccattcgt gcaggctgtc gagcaaggat gcggcggctt cgtcggcaag gttggcaagt
    14161 tcggctttat cggtaagcgc ggcaattttg ccggtaagct gcggcagttt ttccagcgtc
    14221 caaacggcaa tattccatga atgttcggcg gcaaaacggc tgagggcttt tttgggcagg
    14281 tggtgcgctt cgtctatgca atagaaactg ttttcgggcg caggcagaat cacgccgccg
    14341 cccatgctga tgtcggcaag cagaagatcg tggttggcaa caacgacatc gacggtttcc
    14401 aacatatcgc gtgccaggta aaaagggcat tccggacggt tgggacaggc ggctttcagg
    14461 cagccgtggc ggtcgttggt cactttgagc caaatcgcgt catcgatttt ttccggccaa
    14521 gcgtcgcggt cgccgttgaa ccgtcgggcg gaaaattcgt cggcgatgtc gcgcagcagc
    14581 ttcaattctt ccggcttggg tttgctgtcc cacaagacgg cgggggcttc aaagccgagc
    14641 aggttttgct gggcgttgct ttgcgtcagt tgatagagtt tgtaggggca gagatagcgg
    14701 ccgcgccctt tggcaagtgc gaaggtcagt tccaaaccgc ttttttcgac cagaaacggc
    14761 aggtcgcggt ctaccaactg ctcctgcaag gcaaccgtcg cgctgctcac aatcagccgc
    14821 ttgccgcgtg tttgcgccat gatgccgccc gcaagcaggt aggcaagcga tttgcccacg
    14881 ccggtcggcc cttcgattac ggcaatgctt tcgccctcgc gcttgggcgg ctcgccgcct
    14941 tcttcgcgcg tcaacgtccg cgaaaaagcg ttggcaaccg ccgcaatcat ttcccgctgc
    15001 gaagcacgcg gacggaaacc gggcaggttt ttgccgatat tttggtaatg gtcgcggatg
    15061 gcgttttttt ctaaatcggt gagcatagta tcggtgggcg gatgatttgg ggtcatttta
    15121 acattgcgcg gatgcggtac ggtatcgttg tcggacggtg gttcgggcat atctgattat
    15181 tgaaagtaaa aaaaaacgga agatattggg tcatccgtac aaaatgccgt ctgaagcggg
    15241 aaacgggctt cagacggcat tgccgcgctt atacgccggc gacggggcgg gcgggatagc
    15301 cgtgggtatc gaccgaacag gcaaaccgcc aaggcgtgtg gacggtgtcg gcggacaggt
    15361 gggcgagttc gggaatgtgc cgtttgataa aagtaccgtc cgggtcggta cggtatgagg
    15421 cggcggcaat gtcggggcag gtgtgccgtg aggcggcaag ccgccagttg ccttggttga
    15481 ttgccgcatc gaaatcggtc agctgccggg caaaccatat ctcgccttcg cggcggggga
    15541 ggtttaaaac gtggcagaaa aaatccgcgc tcaagcgtct cagggcgggg tggaggctgc
    15601 cggttttgtg caaacagcgc atcgcggcat cgataatcgg aatgccggtc cggccctgct
    15661 gccaaagcgt caggcgcagg gtgtgttccg gattgccgtc tgaagggtcg tcatccgcgt
    15721 gctgcaaggc aagttgaagg aaaaaatcgc ggcggatgat gttgtccgcc cacgcgttca
    15781 gacggcgttc gaggctttcc cgcgcgagca ggcgcggcga gatgcagccg acactcaaat
    15841 acgcgcccat cagcgaagtg tttttgcgcg aagggaaatc ctttaaaacg gagtaggaag
    15901 ccgcctgttc gagaaaccgc cgccactgcc gccaagccgc cgtttcgccg ccgttttgcg
    15961 gcaggaagat gccgtctgaa agcgcggcag gcggcggggc gggcaggttt tcggggaagg
    16021 gttggcggta tgccgcgaat aggtccgaac cggcgggggg ctgcttggaa aagcggtcga
    16081 gccatacttc gcggtagcgg ttgaaatcgg tgtgtgccgt accgttgtct ggtgtcaggt
    16141 cggttttgcc gaaaacggaa cggtcgttga cgaaggttaa cgcgatgccg tgtttgtcca
    16201 attcgtgcca aagggcgttg tcggcgagtt tgtcggcaaa agtatgggat tcgtcggcga
    16261 tgacggcgcg gatattgagg cggacggcga gccggacaag cccggcaggc gatgccgccg
    16321 tgtagagcgg gatgccgcgc cttgccagcc cttgggcgag ttcggcggcg gattggcggt
    16381 agaacgcggc gcggcgcggg ttgtctgttt cggcatcgtc aatccaaatg ccgataacgg
    16441 gcgaaccttc ggcaacggcg gcgcataagg cggcgttgtc gcggatgcgg aggttttggc
    16501 ggaaccagac gagcgtgtgc gcggcgcgcg tgtccgcata aaggaggagg gcggtttcag
    16561 acggcatttc ggcagccttt cctgctggcg attttttcgt tcagaaaatc gatgaaactg
    16621 cggactttcg cactcaaaaa tgccctgtcc gcataaacgg cgttcagccg gtcggtcggg
    16681 acggcgtatc cgggcagcag cctcaccagc gggccgcagc gcaaatcgtg ttccgccgcc
    16741 caaagcggct gataaccgat gcacgcgccc gccttaatca tttcgcgcat catcagcgtg
    16801 ttgtcggtac ggatgacggg ggtcagttca agccggtatt tcttgccgtc cgatttgcgg
    16861 gtgaggtcga gtttctgctg gttggtgtag gtcggcagga cggcaggcag ccccgccact
    16921 tcttccggtg tttccggcac gccgttgcgc ctcaggaagt cgggcgaggc aagcagggca
    16981 aattcgattt ccgccagcgg gcgcgcaatc agcgaagggg aaagggtttg ggaaacgcgc
    17041 aacgccaaat ccacgccttc ggcaatcaaa tcgacgtggc ggttgtccaa aatcagttcc
    17101 aacgccactt cgggataacg ttcgcggtat tccgccagcc agttgcatat ctggctgccg
    17161 gcaaaccaca gcggcatcgt tacgcgcagc agcccctgcg gtttttccgt ccccccggcg
    17221 gctttttgcg cggcatcgtc gagcgtgtcg agcgcgtaac tgcattgccg gtagtattct
    17281 tccccggctt cggtcaggct gaggttgcgg ctgttgtggt gcaggagttt ggcttggacg
    17341 gtgttttcca agtgactgac gtgtttgctt gccattgcgg tggagatgcc gagcgcgtcg
    17401 gcggcgcggg tgaagccgcc gctttggacg acttggcgga aaactttgag gctgaacagg
    17461 gtgtccatat tttcttgtgt ggaaaagttg tatcaataaa agcagtatat atttgaaaaa
    17521 gggaaacatc tatactctgc cgcctgaaat gaagacaaat atcaaaggag cttttatgtc
    17581 cgattgctgc aaccgtatcc aaccggtttt gctttctgtt ttgcgtatcg taaccgccta
    17641 cctgtttttg ttgcacggta cgtcgaaaat cttcgccttc cccattgaaa tgggcagcgg
    17701 ttcgcccggc gggctgttgc tgcttgccgg tattttagaa attgtcggcg gcattttgct
    17761 ggtgttgggc ctgtttgcgc gccctgccgc gtttgttttg tccggtcaga tggcggttgc
    17821 ctattttatg gcgcacgctt ccggaaatgc tttgttcccg attgccaacg gcggcgagtc
    17881 cgcagtgctg ttctgcttcg tatttctcta tatcgcggca gcgggcggcg gagcatggtc
    17941 gctggacagg ctgtttttca agcgtaaagc ctgaaccgga ctgcctaaag tgtattttgt
    18001 tgaatgtttt tgaggaaaag aaatgagccg tcaatccctg caacaggctg ccgaaagccg
    18061 ccgttccgtt tattcgttaa ataaaaacct gcccgttggc aaagatgaaa tcgtccaaat
    18121 cgtcgaacac gccgttttgc acacaccttt ttcgttcaat tcccaatctg cccgcgtggt
    18181 cgtgctgttt ggcgaagagc atgataagat actactttaa ttgacaacag catctttaca
    18241 attcattttc aacccattga atttcaatat tt
//
LOCUS       DAWTQS010000045        18153 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.045, whole genome shotgun sequence.
ACCESSION   DAWTQS010000045 DAWTQS010000000
VERSION     DAWTQS010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18153
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.045"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            <1..1146
                     /locus_tag="WNA25_001435"
     CDS             <1..1146
                     /locus_tag="WNA25_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFA9824167.1"
                     /translation="RGRRSTLPAPPSPSFDSSAYAAPRALHNPDWYENDYEEIPLDAL
                     EDEDVSESVDTSDKQPQDNTELHEKVEAVSLQPRAAQPRTQAAAQADAVSTNTNSALS
                     DAMASTQSILLDTGASLTRHIAQKSRADAEKNSVWMSNTGYGRDYASAQYRRFSSKRT
                     QTQIGIDRSLSENMQIGGVLTYSDSQHTFDQAGGKNTFVQANLYGKYYLNDAWYVAGD
                     IGAGSLRSRLQTQQKANFNRTSIQTGLTLGNTLKINQFEIVPSAGIRYSRLSSADYKL
                     GDDSVKVSSMAVKTLTAGLDFAYRFKVGNLTVKPLLSAAYFANYGKGGVNVGGKSFAY
                     KADNQQQYSAGAALLYRNVTLNVNGSITKGKQLEKQKSGQIKIQIRF"
     gene            complement(1222..2424)
                     /gene="trpB"
                     /locus_tag="WNA25_001436"
     CDS             complement(1222..2424)
                     /gene="trpB"
                     /locus_tag="WNA25_001436"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFA9824168.1"
                     /translation="MKNYHAPDEKGFFGEHGGLYVSETLIPALKELEQAYNEAKNDPE
                     FWAEFRRDLKHYVGRPSPVYHAARLSEHLGGAQIWLKREDLNHTGAHKVNNTIGQALL
                     ARRMGKKRVIAETGAGQHGVASATVAARFGMTCDVYMGADDIQRQMPNVFRMKLLGAN
                     VIGVDSGSRTLKDAMNEAMREWVARVDDTFYIIGTAAGPAPYPEMVRDFQCVIGNEAK
                     AQMQEAIGRQPDVAVACVGGGSNAIGLFYPYIEEENVRLVGVEAGGLGVDTPDHAAPI
                     TSGAPIGVLHGFRSYLMQDENGQVLGTHSVSAGLDYPGIGPEHSHLHDIKRVEYTVAK
                     DDEALEAFDLLCRFEGIIPALESSHAVAWAVKNAPKMGKDQVILVNLSGRGDKDINTV
                     AKLKGIEL"
     gene            complement(2507..3136)
                     /locus_tag="WNA25_001437"
     CDS             complement(2507..3136)
                     /locus_tag="WNA25_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HFA9824169.1"
                     /translation="MSENLLEIETHPFDPVLPPKAAVMMMGTFPPKEDKRAMQFHYPN
                     FQNDMWRVYGLVFFNDAAHFRMPSHGEAAHSEKAFDAEKIKAFLHERGIASCPTVLKA
                     AREHGNASDKFLKVVETVDLAAVLAKIPDCRHICTTGGKATEILLDIQGGGIKMPKTG
                     ETVPFPFAGRDLTLTRLPSTSRAYPLSLAKKAAAYRAFFEMAGLCEKQL"
     gene            3412..4191
                     /gene="rsmA"
                     /locus_tag="WNA25_001438"
     CDS             3412..4191
                     /gene="rsmA"
                     /locus_tag="WNA25_001438"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFA9824170.1"
                     /translation="MKEHKARKRFGQNFLQDTRIIGDIVNAVRPQADDVVIEIGPGLA
                     AITEPLAKKLNRLHVVEIDRDIVCRLKTLPFADKLVIHEGDVLQFDFNGISGKKKIVG
                     NLPYNISTPLLFKLAEVADDVADMHFMLQKEVVERMVAAPKSNDYGRLGVMLQYFFDM
                     ELLIDVPPESFDPAPKIDSAVVRMIPVKHRIGKADDFEHFAKLVKLAFRQRRKTIRNN
                     LKELADDDDLQAVGISPQDRAEHIAPEKYVALSNYLADKAV"
     gene            4206..4775
                     /locus_tag="WNA25_001439"
     CDS             4206..4775
                     /locus_tag="WNA25_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HFA9824171.1"
                     /translation="MESNLQKTERLIREINRLHAQYSQDYFETGKVRKINLSHTLKNV
                     PTEHILSYRLNLHEAVNDYLAFADTRGIDFFYRVKTAESIHDKINRYLARGTQYPTNN
                     ILNDIFGARLIWPSETVAGILEKLDGWKTEYGLKNWYLRDAGGYIGIHIYFKNSSNFY
                     YPWELQVWDEKDAKANIENHMAYKRNFVR"
     gene            4886..5341
                     /locus_tag="WNA25_001440"
     CDS             4886..5341
                     /locus_tag="WNA25_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824172.1"
                     /translation="MKYTAALLTFLLTACMNPNDAFFQNRRYQMPEAQLNGSNAVFHY
                     GYSQNPDHDLLVDQVRVPDAYRASFARDWIAKEQGYRKNDCVLEKAESGGVTYYTCDA
                     EKGFNGTRFVIYVVNMKNDKGYVKVYRGMNKPNQEELTKLVADLNKFYP"
     gene            5383..6111
                     /locus_tag="WNA25_001441"
     CDS             5383..6111
                     /locus_tag="WNA25_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFA9824173.1"
                     /translation="MIKFKNVHKHFKDLHVINGVNLEIKKGEVVVVCGPSGSGKSTLI
                     RTVNQLESIESGEIWVDGVNVADPKTDLNKIREEVGFVFQSFNLYPHLTVLDNITLAP
                     MKVKGQNAEQAEKKAMELLERVGLAHKKDAFPSQLSGGQQQRVAIARGLAMEPRVMLF
                     DEPTSALDPEMVGEVLKVMKDLAESGMTMMCVTHEMGFAREVADRVIFVDKGQILEDE
                     TPEAFFTNPKHERAKQFLQQVMTH"
     gene            6184..6543
                     /locus_tag="WNA25_001442"
     CDS             6184..6543
                     /locus_tag="WNA25_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824174.1"
                     /translation="MYFVDRTAAVLKPTARFLEWLKSTDENMPDLTIEQLRADCSVFL
                     VPQFDEPEAVVSYFDERYRQIFEAELAGWDIDKDKWPQDMGLKAFWEFFDIEIHDMVL
                     DMEEADLNITPVFDNMM"
     gene            6548..6988
                     /locus_tag="WNA25_001443"
     CDS             6548..6988
                     /locus_tag="WNA25_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FolI"
                     /protein_id="HFA9824175.1"
                     /translation="MRAFQTALRPSRILNILTVSLHSASIAVCLTWFYGRMMWFGLAA
                     LAASYAYSLRITNLKHRHAITAITIDRDGRAEIVSGKDKTAATLAGSSMVTPYALFLQ
                     WDTGGKTVRQCIMPDMTDKESYRRLKVWVLWRQPKKTAETDTSD"
     gene            7019..8293
                     /gene="folC"
                     /locus_tag="WNA25_001444"
     CDS             7019..8293
                     /gene="folC"
                     /locus_tag="WNA25_001444"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFA9824176.1"
                     /translation="MKTLQDWLSHLETAHSGGLIDMGLERVSEVKKRMNLTPQCPVVV
                     VAGTNGKGSVCAYLTQIYKQAGFKTGTLTSPHLLHYNERIAINAEPVSDDTIIASFER
                     IEAARGEISLTYFEFNALAAVDIFIREQVDVMILEVGLGGRLDAVNAFDGDCAVVTSV
                     DLDHQAFLGDTVEQVGFEKAGVFRSGKPAICGQNPAPASLVAHAEAIGAKLLMVQRDF
                     EFHAMENIQWNYRFRPQHSDGPARNRNALPFPALRGAYQLSNAACALTVLECLDDRLP
                     VDIGAIKRGLLLVENPGRFQVLPGRPLTVLDVGHNPHAARALRRNLINLAYAQKRTAV
                     FSMLSDKDIDGVSETVKDQFDEWYIAPLDVPRGMTADALKAKLEQHHIENIQTFAAVR
                     DAYRAAASKAGEDDRIVVFGSFHTVADVMSVL"
     gene            8307..9347
                     /gene="ftsN"
                     /locus_tag="WNA25_001445"
     CDS             8307..9347
                     /gene="ftsN"
                     /locus_tag="WNA25_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFA9824177.1"
                     /translation="MSENKQNEVLTGYEQLKRRNRRRLVTASSLVAASCILLAAALSS
                     DPADSNPAPQAGETGATESQTANTAQTPALKSAAENGETAADKPQDLAGEDKPSAADS
                     EISEPENVGAPLVLINDRLEDSNIKGLEESEKLQQAETAKTEPKQAKQRAAEKVSATA
                     DSTDTVAVEKPKRTAEPKPQKAERTAEAKPKAKETKTAEKVADKPKTAAEKTKPDTAK
                     SDSAVKEAKKADKAEGKKTAEKDRSDGKKHETAQKTDKADKTKTAEKEKSGKAGKKAA
                     IQAGYAEKERALSLQRKMKAAGIDSTITEIMTDNGKVYRVKSSNYKNARDAERDLNKL
                     RVHGIAGQVTNE"
     gene            9340..9837
                     /locus_tag="WNA25_001446"
     CDS             9340..9837
                     /locus_tag="WNA25_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFA9824178.1"
                     /translation="MNSLPIADLLASAVIAACIVISTMRGVIAEAGSMVAWVVSFFFA
                     KLFAAPFADLAFASFQPRLFALALSFISLFVIACLIQKMLRSLLTGAVSAVGLGFANR
                     ILGGVFGALKGVLIVTLLIMLASKTDLPDTEEWQQSYTVPFFVSLSEAVLNHTDNAPE
                     SLDDD"
     gene            10031..11575
                     /gene="purF"
                     /locus_tag="WNA25_001447"
     CDS             10031..11575
                     /gene="purF"
                     /locus_tag="WNA25_001447"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFA9824179.1"
                     /translation="MCGVLGLVSHEPVNQLLYDGLQMLQHRGQDAAGIATAEGGTFHM
                     HKGKGMVSEVFRTRNMRDLTGNAGIAHVRYPTAGNAGSSAEAQPFYVSSPFGIVLAHN
                     GNLTNTAELYENVCNKHLRHINTGSDSEVLLNVFAHELRREVSKNADPHRLNADNIFN
                     AVAEVHRLVRGAYGVVAMIAGYGMLAFRDPYGIRPLALGSQTDSEGRKSYAVASESVA
                     FNALAYDLERDIRPGEAVFVGFNGTIIARQCSDRAKLSPCLFEYVYFARPDSVIDGVS
                     VYQSRLDMGVSLAEKIKRELPVDGIDVVMPIPDTSRPSAMELAVHLNKPYREGLIKNR
                     YIGRTFIMPGQSTRKKSVRQKLSPMETEFAGKSVLLVDDSIVRGTTSREIVEMVRAAG
                     ARKVYIASAAPEVRYPNVYGIDMPTREELIANGRSAAEIAAEIGADGIVFQDLGDLEA
                     VVKALNPKIESFDSSCFNGIYQTGDIDDAYLDRLSAEKSGCGGLKIHPSRMEHSISIS
                     DAGDEE"
     gene            11675..12166
                     /gene="greB"
                     /locus_tag="WNA25_001448"
     CDS             11675..12166
                     /gene="greB"
                     /locus_tag="WNA25_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFA9824180.1"
                     /translation="MSTETKNYITPAGWQALKDELYQLVNKERPEIVQIVNWAAGNGD
                     RSENGDYLYGKRRMREIDRRIRFLTKRLEAAVVVDPELREATDQVFFGATVGLLRGDG
                     REQTVKIVGVDEIDTAQNKISWISPLARCLIKAREGDEVVLNTPEGREEIEILSVEYI
                     KID"
     gene            12225..12851
                     /locus_tag="WNA25_001449"
     CDS             12225..12851
                     /locus_tag="WNA25_001449"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HFA9824181.1"
                     /translation="MRKIRTKICGITTPEDALYAAHAGADALGLVFYPQSPRAIDIIK
                     AQKIAAALPPFVSVVALFINESAQNIRRILAEVPIHIIQFHGDEDDAFCRQFDRPYIK
                     AIRVQTASDIRNAATRFPNAQALLFDAYHPSEYGGTGHRFDWTLLAEYSGKPWVLAGG
                     LTPENVGEAVRITGAEAVDVSGGVEASKGKKDPAKVAAFIATANRLSR"
     gene            complement(13325..16156)
                     /gene="lbpA"
                     /locus_tag="WNA25_001450"
                     /gene_synonym="tbpA"
     CDS             complement(13325..16156)
                     /gene="lbpA"
                     /locus_tag="WNA25_001450"
                     /gene_synonym="tbpA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin/transferrin-binding protein LbpA"
                     /protein_id="HFA9824182.1"
                     /translation="MNKKHGFPLTLTALAIATAFPAYAAQAGAAALDAAQSQSLKEVT
                     VRAAKVGRRSKEATGLGKIVKTSETLNKEQVLGIRDLTRYDPGVAVVEQGNGASGGYS
                     IRGVDKNRVAVSVDGVAQIQAFTVQGSLSGYGGRGGSGAINEIEYENISTVEIDKGAG
                     SSDHGSGALGGAVAFRTKEAADLISDGKSRGIQAKTAYGSKNRQFMKSLGAGFSKDGW
                     EGLLIRTERQGRETRPHGDIADGVEYGIDRLDAFRQTYDIKRKTTEPFFLVEGENTLK
                     PVAKLAGYGIYLNRQLNRWVKERIEQNQPLSAEEEAQVREAQARHENLSAQAYTGGGR
                     ILPDPMDYRSGSWLAKLGYRFGGRHYVGGVFEDTKQRYDIRDMTEKQYYGTDEAEKFR
                     DKSGVYDGDDFRDGLYFVPNIEEWKGDKNLVKGIGLKYSRTKFIDEHHRRRRMGLLYR
                     YENEKYSDNWADKAVLSFDKQGVATDNNTLKLNCAVYPAVDKSCRASADKPYSYDSSD
                     RFHYREQHNVLNASFEKSLKNKWTKHHLTLGFGYDASKAISRPEQLSHNAARISESTG
                     FDEKNQDKYRLGKPEVVEGSVCGYIETLRSRKCVPRKINGSNIHISLNDRFSIGKYFD
                     FSLGGRYDRKNFTTSEELVRSGRYADRSWNSGIVFKPNRHFSVSYRASSGFRTPSFQE
                     LFGIDIYHDYPKGWQRPALKSEKAANREIGLQWKGDFGFLEISSFRNRYTDMIAVADQ
                     KTKLPDSAGRLTEIDIRDYYNAQNMSLQGINILGKIDWNGVYGKLPEGLYTTLAYNRI
                     KPKSVSNRPDLSLRSYALDAVQPSRYVLGFGYDQPEGKWGANIMLTYSKGKNPDELAY
                     LAGDQKRYSAGRVTSSWKTADVSAYLNLKKRLTLRAAIYNIGNYRYVTWESLRQTAES
                     TANRHGGDSNYGRYAAPGRNFSLALEMKF"
     gene            complement(16153..18138)
                     /locus_tag="WNA25_001451"
     CDS             complement(16153..18138)
                     /locus_tag="WNA25_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFA9824183.1"
                     /translation="MRLLRRNTAFHREDGTAIPDSKQAEEKLSFKEGDVLFLYGSKGN
                     KLQQLKSEIHKRDSDVEIRTSEKENKKYNYKFVDAGYVYTKNGKDEIEQNSGGKRFTH
                     RFGYDGFVYYSGERPSQSLPSAGTVKYFGNWQYMTDAKRHRTGKAVASDDLGYITFYG
                     NDIGATSYAAKDADDREKHPAEYTVDFDKKILKGELIKNQYVQKRNDPKEPKKPLTIY
                     NITADLNGNRFTGSAKVNTEVKTRHADKEYLFFHTDADQRLEGGFFGDNGEELAGRFI
                     SNDNGVFGVFAGKQKTNASGTNPAMPFGKHTKILDSLKISVDEATDENPRPFEVSTMP
                     DFGHPDKLLVEGREIPLVSKEKTIDLADGRKMTVSACCDFLTYVKLGRIKTERPAVKP
                     KAQDEEDSGINNGEESEDEEIAEESEDEVSEDDNGEDEDEIVEEEADEAEEIEEEAEE
                     EEPEEESPEEGNGVSDGIPPAPEALKGRDIDLFLKGIRTAEADIPKTGTAHYTGTWEA
                     RIGEPIQWDNKADKAAKAEFDVDFGNKSISGTLTEQNGVEPAFRIENGVIEGNGFHAT
                     ARTRDNGINLSGNGSTNPQSFKADNLLVTGGFYGPQAAELGGTIFNKDGKSLGITEDI
                     ENEVENEADVGEQLEPEVKPQFGVVFGAKKDNKEVEK"
BASE COUNT         4437 a         5046 c         4495 g         4175 t
ORIGIN      
        1 cgcggacgca gatctactct gccggcaccg ccctcgccat catttgattc atcagcttac
       61 gcagcaccca gggccttgca taatccggac tggtatgaaa atgattatga agaaatcccc
      121 ttggatgcgt tggaagatga agatgtatcc gaatcggttg acacatcaga caaacagcct
      181 caagacaata cggaacttca tgaaaaagtt gaggcagtga gtttgcaacc aagagccgcg
      241 cagccgcgaa cccaagccgc cgcgcaagcc gatgcagtca gcaccaatac taactcggct
      301 ttatctgacg caatggcaag cacgcaatct atcttgttgg atacaggtgc ttcattaaca
      361 cggcacattg cacaaaaatc acgcgctgat gccgaaaaaa acagtgtttg gatgtcaaac
      421 accggttatg gccgtgatta tgcttccgca caatatcgcc ggtttagttc gaaacgcacg
      481 caaacacaaa tcggcattga ccgcagcttg tccgaaaata tgcagatagg cggagtattg
      541 acttactctg acagtcagca tacttttgat caggcgggcg gcaaaaatac ttttgtgcaa
      601 gccaaccttt atggtaagta ttatttaaat gatgcttggt atgtggccgg cgatattggt
      661 gcgggcagct tgagaagccg gttacaaacg cagcaaaaag caaactttaa ccgaacaagc
      721 atccaaaccg gccttacttt gggcaatacg ctgaaaatca atcaattcga gattgtccct
      781 agtgcgggta tccgttacag ccgcctgtca tctgcagatt acaagttggg tgacgacagt
      841 gttaaagtaa gttctatggc agtgaaaaca ctaacggccg gactggattt tgcttatcgg
      901 tttaaagtcg gcaaccttac cgtaaaaccc ttgttatctg cagcttactt tgccaattat
      961 ggcaaaggcg gcgtgaatgt gggcggtaaa tccttcgcct ataaagcaga taatcaacag
     1021 caatattcag caggcgccgc gttactgtac cgtaatgtta cattaaacgt aaatggcagt
     1081 attacaaaag gcaaacaatt ggaaaaacaa aaatccggac aaattaaaat acagattcgt
     1141 ttctaaaata ccaaattcat agcaaaataa aatgccgtct gaactcaagc ttcggacggc
     1201 atttttatca gactaacaaa gctacagctc aatgcctttg agtttcgcca cggtattgat
     1261 gtctttgtcg ccgcgacccg acaggttgac caaaatcact tggtctttac ccattttcgg
     1321 cgcgtttttc accgcccaag caacggcgtg gctggattcg agcgcgggga tgatgccctc
     1381 gaatcggcag agcaagtcaa aggcttcgag tgcttcgtca tctttggcaa cggtgtattc
     1441 gacgcgcttg atgtcgtgca gatggctgtg ttccgggccg atgccggggt aatccaagcc
     1501 tgcggaaaca gagtgcgtcc ccaagacttg gccgttttcg tcctgcatca gatagctgcg
     1561 gaagccgtgc agtacgccaa tcggtgcgcc cgaagtaatc ggcgcggcgt ggtcaggcgt
     1621 atccacgccc aaaccgcctg cttctacgcc gaccaaacgt acgttttctt cttcgatata
     1681 gggatagaac aaaccgatgg cgttcgagcc gccgccgacg caggcaacgg cgacgtcggg
     1741 ctgtctgccg atagcttcct gcatctgcgc tttggcttcg ttgccgatga cgcattggaa
     1801 atcacgcacc atttcgggat acggcgcggg gccggcggcg gtgccgatga tgtagaacgt
     1861 atcgtccact cgggcgaccc attcgcgcat cgcttcgttc atcgcgtctt tcaaagtacg
     1921 gctgccactg tcaacgccga tgacattcgc accaagcagc ttcatacgga acacattcgg
     1981 catttggcgt tgaatatcgt ccgcacccat gtacacgtcg caagtcatgc cgaaacgtgc
     2041 ggcgacggtg gcagaagcca cgccgtgctg acccgcgccg gtttcggcga tgacgcgttt
     2101 tttgcccata cggcgggcaa gcaatgcctg accgatggtg ttgttgacct tgtgcgcgcc
     2161 ggtgtggttc aagtcttcgc gcttcaacca gatttgcgcg ccgcccaaat gctcggacaa
     2221 ccgcgcggcg tggtaaacgg ggctgggcct gccgacataa tgtttcaaat cgcggcggaa
     2281 ctccgcccaa aattcaggat cgtttttcgc ttcgttgtag gcctgttcca gctctttcaa
     2341 agcgggaatc agggtttcgg aaacataaag cccgccgtgt tcgccgaaaa agcccttttc
     2401 gtcgggcgcg tggtagttct tcatcggatt cttccttatg cttcaggttt tcagacggca
     2461 tttccgtttt ttcaaacatg ccgtctgaac gggaaattgt cggcaattat aactgctttt
     2521 cacacaagcc cgccatttcg aaaaacgccc gatacgccgc tgcctttttc gccaaactca
     2581 aaggataggc gcgcgaagtc gaaggcaggc gcgtcagtgt caaatcccgt ccggcaaacg
     2641 gaaacggcac ggtttcgccc gttttcggca ttttgatgcc gccgccctga atatcgagca
     2701 ggatttccgt cgccttgccg cctgtcgtac aaatatggcg gcagtctggt attttcgcca
     2761 acaccgccgc caaatcgacg gtttcaacta cctttaaaaa cttgtcggac gcattgccgt
     2821 gttcccttgc cgccttcaac acggtcggac aggacgcaat cccccgttcg tgcaaaaacg
     2881 ccttgatttt ctccgcgtca aacgcttttt cagagtgcgc agcctccccg tgggacggca
     2941 tccggaaatg cgccgcatcg ttaaaaaaca ccagcccgta aacgcgccac atatcgtttt
     3001 ggaaattcgg ataatgaaac tgcatcgcgc gtttgtcttc cttgggcgga aacgtcccca
     3061 tcatcatgac cgcagccttc ggcggcaaca cgggatcgaa gggatgggtt tcgatttcaa
     3121 gcagattttc agacacgttc aatcctccaa caggcaaagc ggcagacagc cggtttgcac
     3181 caaaccgcca aacacggcaa gccttcaaac agcattatca cgccctgccg ttttcggtta
     3241 caatgccgac atcaaacgga tactgtaaac acatttatgt tccaacacac aggacgacac
     3301 ataaagcacc gccctatgtg tcgtcctgat ttggaagggg ttacgccccg cccaaacaga
     3361 gtctgatcct gccgccctaa agggcggggt ttcaaccgaa gaggaaacac gatgaaagaa
     3421 cacaaagccc gcaagcgttt cgggcagaat tttttgcagg acacgcggat tatcggcgat
     3481 attgtcaacg ccgtgcgccc gcaggcggat gatgtcgtga ttgaaatcgg cccgggtttg
     3541 gcggcaatta ccgaaccttt ggcgaaaaag ctgaaccgcc tgcacgttgt cgaaatcgac
     3601 cgcgacatcg tatgccgtct gaaaacgctg ccgtttgcgg ataaactggt gattcacgaa
     3661 ggcgacgtgt tgcagtttga tttcaacggc atctcaggca aaaagaaaat cgtcggcaac
     3721 ctgccgtata acatctccac gccgcttttg ttcaaactgg cggaggtggc ggacgatgtc
     3781 gccgatatgc actttatgtt gcaaaaagaa gtggtcgagc gcatggttgc cgcgccgaaa
     3841 agcaacgact acggtcgctt gggtgtgatg ctgcaatatt ttttcgatat ggagctgctg
     3901 attgacgtgc cgcccgaatc gttcgatccc gcgccgaaaa tcgattccgc agtggtgcgc
     3961 atgattccgg tgaaacaccg tatcggcaag gcggacgatt tcgagcattt cgccaaactc
     4021 gtgaaactcg ccttccgcca acgccgcaaa accatacgca acaatctgaa agagcttgcc
     4081 gacgacgatg atttgcaggc agtcggcatc agtccgcaag accgtgccga acacatcgcg
     4141 ccggagaaat atgtggcgtt gagcaattat ctggcggaca aggccgtctg aaaaggaacg
     4201 cggcgatgga aagcaacctg cagaaaaccg agcgtttgat ccgcgaaatc aaccgcctgc
     4261 acgcacaata ttcgcaggat tattttgaaa ccggcaaagt gcggaaaatc aatttgagcc
     4321 acacgctcaa aaacgttccg accgaacata ttttgtctta ccgccttaat ttgcacgaag
     4381 cagttaacga ttatttagcc tttgccgata cgcgcggcat agactttttc taccgcgtga
     4441 aaaccgcaga aagcatacat gataaaatca accgctatct ggcacgcggc acccaatatc
     4501 cgaccaacaa catcctgaac gatattttcg gcgcgcggct gatttggccg tctgaaacag
     4561 tcgccgggat tttagaaaaa cttgacggct ggaaaacaga atacggcttg aaaaactggt
     4621 atttgcgcga tgccggcggc tatatcggca tacacattta tttcaaaaac agcagcaatt
     4681 tctactatcc gtgggaattg caggtttggg atgaaaaaga tgccaaagcc aatattgaaa
     4741 accatatggc gtacaaacgg aatttcgtgc gttgaattgc cggcaagccg ccttgaaacc
     4801 gtttataact gtttataaga gaataaacca taaaatacac aaacgtattc ctgacttttc
     4861 ctttaaccgc ccgagtgaac aaactatgaa atacacagcc gcactcctga cttttctttt
     4921 aactgcctgc atgaacccga acgatgcgtt tttccaaaac cggcgttatc aaatgccgga
     4981 ggcgcaattg aacggttcaa acgccgtttt ccattacggt tattcccaaa atcccgatca
     5041 tgatttattg gtcgaccaag tccgcgtgcc ggacgcgtac agggcttcat ttgcgcggga
     5101 ttggatagcg aaggagcaag gttaccgcaa aaacgactgc gtactggaaa aagcggaaag
     5161 cgggggtgtg acgtactaca cctgcgatgc ggaaaaagga ttcaacggca cacgtttcgt
     5221 catctatgtc gtgaatatga aaaacgacaa aggctacgtc aaagtttacc ggggcatgaa
     5281 taagccgaat caggaagagt taaccaaatt ggttgccgat ttgaataagt tttaccctta
     5341 atcagccaga cggattcaga ctgcccgaaa agaatggaca ctatgatcaa attcaaaaac
     5401 gtacacaaac atttcaaaga cctgcacgtc atcaacggcg taaacttgga aatcaaaaaa
     5461 ggcgaagtgg tcgtcgtctg cgggccttcg ggcagcggca aatccacact catccgcacc
     5521 gtcaaccagc ttgaaagcat cgaaagcggc gagatttggg tggacggcgt caacgttgcc
     5581 gaccccaaaa ccgatttgaa caaaatccgc gaagaagtcg gctttgtgtt tcaaagtttc
     5641 aacctctacc cgcacctgac cgtgttggac aacatcaccc tcgcaccgat gaaggttaag
     5701 gggcaaaatg cggaacaggc ggagaaaaag gcaatggagc ttttggaacg cgtcggactg
     5761 gcacacaaaa aagacgcctt cccttcccaa ctttccggcg gccagcagca acgcgtggcg
     5821 attgcgcgcg ggctggcgat ggagccgcgc gtgatgctgt ttgacgagcc gacttccgca
     5881 ctcgaccccg aaatggtcgg cgaagtgttg aaagtgatga aagacttggc ggaaagcggc
     5941 atgacgatga tgtgcgtaac ccacgaaatg gggtttgccc gcgaagttgc cgaccgcgtg
     6001 attttcgtcg ataaagggca aatccttgaa gacgaaacgc cggaagcgtt tttcaccaac
     6061 ccgaaacacg aacgcgccaa gcagttcctg caacaagtca tgacccattg atgctatgcc
     6121 gtctgaaaac cgaacgttca gacggcatag cccgacaccg caagatgatt gagaaagcac
     6181 accatgtatt ttgttgaccg caccgccgcc gtcctcaagc cgaccgcgcg ctttttggaa
     6241 tggctcaaaa gcaccgacga aaatatgccc gacctgacca tagaacagct tcgcgccgac
     6301 tgttccgtct tcctcgttcc gcagttcgac gaaccggaag ccgtcgtttc ctacttcgac
     6361 gaacgctacc ggcagatttt tgaggcggag ctggcaggtt gggacatcga caaggacaaa
     6421 tggccgcaag atatggggct gaaggcgttt tgggaatttt tcgacattga aatccacgat
     6481 atggttttgg atatggaaga agcggatttg aacatcacgc ccgtgttcga caatatgatg
     6541 taacgccatg cgcgcctttc agacggcatt gaggccgtcc cgcatcctga acatcctgac
     6601 cgtatcactc cactcggctt ccattgccgt ctgcctgact tggttttacg gccggatgat
     6661 gtggttcggt ttggcggctt tggcggcaag ttacgcatac tcgctcagga taacgaatct
     6721 gaaacaccgc catgccataa ccgccattac catagaccgc gacggacggg cggaaatcgt
     6781 atccggcaaa gacaaaacag cggcaacact tgcgggcagc agtatggtta cgccttatgc
     6841 cttattctta caatgggaca caggcggcaa aaccgtccgc caatgcatca tgcccgacat
     6901 gacggacaaa gaatcctacc ggcggctgaa agtctgggtg ctttggcggc agccgaaaaa
     6961 aaccgccgaa accgatacat cagactgatt tcagacggca tttgaaaacg aaacactcat
     7021 gaaaacatta caagactggc tctcgcattt ggaaaccgcc cacagcggcg gtttgatcga
     7081 catgggtttg gagcgcgtaa gcgaagtaaa aaagcgcatg aacctgacac cgcaatgccc
     7141 cgtcgtcgtc gtcgcgggaa caaacggcaa aggttcggtc tgcgcctacc tgacacagat
     7201 ttacaaacag gcaggcttca aaaccggcac gctgaccagc ccgcatttat tgcattacaa
     7261 cgaacgcatc gccatcaacg ccgaacccgt ttcggacgat acgattattg cctctttcga
     7321 gcgcatcgaa gccgcgcgcg gcgaaatatc gctgacttat tttgaattca atgccttggc
     7381 ggctgtcgac attttcatac gcgaacaagt ggatgtaatg atattggaag tcggcttggg
     7441 cggacgtttg gacgcggtca acgcgtttga cggcgattgc gcggtggtta ctagtgtgga
     7501 tttggaccat caggcatttt tgggcgatac ggtcgaacag gtcggctttg aaaaggcagg
     7561 cgtgttccgc agcggcaaac ccgccatctg cggtcaaaac cccgcgcccg catcattggt
     7621 cgcacacgcc gaagccatag gcgcgaaact gctgatggta cagcgcgatt tcgagtttca
     7681 cgcgatggaa aacatccaat ggaactaccg cttccggccg cagcattcag acggtcccgc
     7741 acgcaaccgc aatgccctgc ccttccccgc attgcgcggc gcataccagc tttctaacgc
     7801 cgcctgcgcg ttgaccgtat tggaatgcct cgacgacaga ctgccggtgg acattggtgc
     7861 aatcaaacgc gggctgctgc tggttgaaaa tcccggacgc ttccaagtcc tgcccggccg
     7921 gccgctgacc gtattggatg tcggacacaa cccccacgcc gcccgcgccc tgcgccgcaa
     7981 cctaatcaac cttgcctacg cgcaaaagcg caccgccgtg ttcagtatgc tgtccgacaa
     8041 agacatagac ggcgtgtcgg aaaccgttaa agaccagttt gacgagtggt atatcgcgcc
     8101 tttggacgtg ccgcgcggta tgacggcgga tgcactgaaa gccaaattgg aacaacacca
     8161 catcgaaaac atacagacat tcgccgccgt ccgcgacgcg taccgggcgg cagcctcaaa
     8221 agcgggcgaa gacgacagaa tcgtcgtatt cggctcattt cataccgtcg ccgacgtaat
     8281 gtcggtactg tgaaaaggaa acttctatgt ccgaaaacaa acaaaacgaa gtcctgaccg
     8341 gttacgaaca gctgaaacgg cgcaaccgcc gccgcctcgt aacggcaagc tccctggttg
     8401 ccgcctcctg catcctgctg gcagccgcac tcagttccga tcctgccgac agcaatcccg
     8461 caccgcaggc cggcgaaacc ggcgcaacgg aaagccaaac ggcaaacacg gcacaaaccc
     8521 ctgccttgaa atccgccgcc gaaaacgggg aaaccgccgc cgacaaaccg caggacttgg
     8581 caggcgaaga caagccttct gccgccgaca gcgaaatcag cgagcctgaa aacgtaggcg
     8641 cgccgctggt gctgattaac gaccggctcg aagacagcaa catcaaaggt ttggaagaat
     8701 ccgagaaact gcaacaggca gaaaccgcca aaaccgaacc gaagcaggca aaacaacgcg
     8761 ctgccgaaaa agtgtcggca actgccgaca gtacggatac ggtagcggtt gaaaaaccga
     8821 aacgcactgc cgaacccaaa ccgcaaaaag cggaacgcac tgccgaagcc aagcccaaag
     8881 ccaaagaaac caaaaccgcc gaaaaagttg ccgacaaacc gaaaactgct gccgaaaaaa
     8941 ccaaaccgga tacggcaaaa tccgacagcg cggtaaaaga agcgaaaaaa gccgacaagg
     9001 ctgaaggcaa aaagacagcc gaaaaagacc gttcggacgg caaaaaacac gaaacggcgc
     9061 aaaaaaccga caaagcggac aaaaccaaaa ccgccgagaa ggaaaaatcc ggcaaggcgg
     9121 gcaaaaaagc cgccattcag gcaggttatg ccgaaaaaga acgcgccttg agcctccagc
     9181 gcaaaatgaa ggcggcgggt atcgattcga ccatcaccga aatcatgacc gacaacggca
     9241 aagtttaccg cgtcaaatca agcaactata aaaacgcaag ggatgccgaa cgcgatttga
     9301 acaaactgcg cgtgcacggc atcgccggcc aggtaacgaa tgaatagcct ccccattgcc
     9361 gacctcctcg cctccgccgt catcgccgcc tgcatcgtca tttccacgat gcgcggcgtg
     9421 attgcggaag caggttcgat ggtggcatgg gtggtttcct tcttttttgc caaactcttt
     9481 gccgcaccct tcgccgacct cgcctttgcc tcgttccaac cccgcctgtt tgcattggct
     9541 ctgtcattca tttccctgtt cgtcattgcc tgtctgatcc agaaaatgct ccgttcgctg
     9601 ctgaccggcg cagtttcggc ggtcggtctg ggctttgcca accgcatttt gggcggtgta
     9661 ttcggtgcat tgaaaggcgt tttgattgtt accctgctga tcatgcttgc ttcaaaaacc
     9721 gacctgcccg ataccgaaga atggcaacag tcctataccg taccgttttt cgtatcgctt
     9781 tccgaagcgg tgttaaacca tacggacaac gcacccgaat ccctcgacga cgactaatcc
     9841 gcatacgtgg cgtttcagac agcatacctg atttatgccg tccgaaacgc cttttctttc
     9901 cttgacacac cccgacagac aaagcgcacc tgcctgactt tacacaaata ttgtcaacaa
     9961 ttcgggcaaa aagccgccgt ttgctttata attccgtttc acccttaaac ctcgaatcgg
    10021 agaagacgat atgtgcggcg tattaggttt ggtcagtcat gaacccgtga accagcttct
    10081 gtacgacggt ttgcagatgt tgcagcacag ggggcaggat gcggcgggca ttgcaacggc
    10141 ggaaggcggt accttccata tgcacaaagg caaagggatg gtgagcgaag tgttccgcac
    10201 gcgcaatatg cgcgatttga ccggcaacgc cggcatcgcc cacgtccgtt atcccacggc
    10261 gggcaacgcc ggcagcagcg cggaggcgca gcctttctac gtcagctcgc ctttcggcat
    10321 cgttttggcg cacaacggca acctcaccaa cactgccgaa ctgtatgaaa acgtgtgtaa
    10381 caaacacctg cgccacatca acaccggctc cgattccgaa gttttgctca acgtattcgc
    10441 gcacgaattg cgccgcgaag tctctaaaaa cgccgacccg caccggctca atgccgacaa
    10501 tattttcaac gccgttgccg aagtccaccg cctggtgcgc ggtgcatacg gcgtggttgc
    10561 catgattgcg ggctacggta tgctcgcctt ccgtgatcct tacggcatcc gcccgctggc
    10621 attgggttcg caaaccgaca gcgaaggcag gaaatcctat gccgtcgcct ccgaatccgt
    10681 cgccttcaac gcgcttgcct acgatttgga acgcgatatc cggccgggcg aagcggtatt
    10741 tgtcggcttt aacggcacaa tcatcgcccg acaatgcagc gaccgcgcca aactcagccc
    10801 ctgccttttt gaatatgttt attttgcccg ccccgactcc gtgattgacg gcgtgtccgt
    10861 ttaccagtcg cgcttggata tgggcgtgtc cttggcggaa aaaatcaaac gcgagctgcc
    10921 cgtggacggc atcgacgtcg tgatgcccat tcccgatacc agccgcccca gtgcaatgga
    10981 gcttgccgtc cacctcaaca aaccctaccg cgagggtttg attaaaaacc gctatatcgg
    11041 ccgcaccttt atcatgcccg gtcagtcgac gcgcaaaaaa tccgtgcgcc aaaaactcag
    11101 cccgatggaa accgaatttg caggcaaaag cgtgttgctg gtggacgact ccatcgtgcg
    11161 cgggacgacc agccgcgaaa tcgtcgaaat ggtacgcgcg gcgggcgcgc gcaaagtcta
    11221 tatcgcctcc gccgcgcccg aagtgcgcta tcccaatgtg tacggcatcg atatgcccac
    11281 gcgcgaagag ttgattgcca acgggcgcag cgcggcggaa atcgccgccg aaatcggcgc
    11341 ggacggcatc gtgtttcaag acttgggcga tttggaagcc gtcgtcaaag cactcaaccc
    11401 gaaaatcgaa tccttcgatt catcctgttt caacggcatt tatcagaccg gcgacatcga
    11461 tgatgcctac ctcgaccgcc tgtctgccga gaaatccggc tgcggcggtc tgaaaatcca
    11521 cccgagcagg atggaacaca gcatcagcat cagcgatgcg ggcgacgaag aataaggctt
    11581 cccaaaccgc gatgccgtct gaaatccgaa cgttgggttt tcagacggca tttcccatat
    11641 cggcatcaca tttcccgttt catccgcaca cgatatgagt accgaaacca aaaactacat
    11701 tacgcccgcc ggctggcagg cattgaaaga cgagctttat caactggtca ataaagaacg
    11761 ccccgaaatc gtccaaatcg tcaactgggc ggcaggcaac ggcgaccgca gcgaaaacgg
    11821 cgactatctt tacggcaaac gccgtatgcg cgaaatcgac cgccgcatcc gtttcctgac
    11881 caaacgtttg gaggctgccg tcgtcgtcga tcccgaattg cgcgaagcga ccgaccaagt
    11941 atttttcggc gcgaccgtcg gtctgttgcg cggcgacggg cgcgagcaaa ccgtcaaaat
    12001 cgtcggcgtc gatgaaatcg ataccgcgca aaacaaaatt tcttggattt cgccgttggc
    12061 gcgttgcctg attaaagcgc gcgaaggcga cgaagtggtt ttgaacacgc cggaaggccg
    12121 tgaggaaatt gaaatccttt ctgtcgaata catcaaaatc gactgatcgg gaatgccgtc
    12181 tgaaacggca caagcgcacc gtttcccaaa taaaggaata ccgaatgagg aaaatccgca
    12241 ccaaaatctg cggcatcacc acaccggaag acgcactgta tgccgcccac gccggcgcag
    12301 acgcattggg actggttttt tacccccaaa gcccccgcgc tatcgacatc attaaagcac
    12361 aaaaaatcgc cgccgcactg ccgccgtttg tcagcgttgt cgcccttttc atcaacgaaa
    12421 gcgcgcaaaa catccgccgc atccttgccg aagtgccgat acacatcatc caattccacg
    12481 gcgacgaaga cgatgcattc tgccggcagt tcgaccgccc ctatattaaa gccattcgtg
    12541 ttcagacggc atcagacatc cgaaacgccg ccacgcgctt ccccaacgct caggcactgc
    12601 tgttcgatgc ctatcaccct tcggaatacg gcggcaccgg acaccgcttc gactggacgc
    12661 tgttggcgga atattcgggc aagccgtggg tgcttgccgg cgggctgacc cctgaaaacg
    12721 tcggcgaagc cgtccgcatc accggagcgg aagcggtcga cgtatccggc ggcgtggaag
    12781 cgtctaaagg caaaaaagac cccgccaaag tcgccgcctt tatcgcaacc gccaaccgcc
    12841 tatcccgtta aagcaacaaa aattgccgcc ggaatgactt atagtggatt aacaaaaacc
    12901 agtacggcgt tgcctcgcct tgccgtacta tttgtactgt ctgcggctcg ccgccttgtc
    12961 ctgatttttg ttaatccact ataatctcaa aaatttatgc cattaaatca ataatttctg
    13021 atgaattttg aaaacttaat cccgtcattc ccgcgcaggc gggaacccgg ttcattgagc
    13081 ttcagctatt tagaataaat tttgaaactc taatcgcgtc attcccacga aagtgggaat
    13141 ccggtttttt gagtttcagt catttccgat aaattgcctt agcattgaat gtccggattc
    13201 ccgcctgcgc gggaatgacg gcggaaatct tgtttatatt gaatcaaaaa aacctgcacc
    13261 ttaatcagtt ggcggtttag tccgactttt ggggtgcaga tcaagcttcc agacggcatt
    13321 tcctttaaaa cttcatttcg agcgcgaggc tgaagttcct gcccggtgcg gcataccttc
    13381 catagttgct gtcgccgccg tgccggtttg ccgtgctttc cgcagtctgg cgcaaggatt
    13441 cccaagtaac gtagcggtag ttgccgatat tgtagatagc cgccctcaag gtcagccgtt
    13501 ttttcagatt cagataagcg gaaacatctg ccgttttcca agaagacgta acccttcctg
    13561 ccgaatatcg tttttgatcg cctgccagat aagcaagctc gtcagggttt ttccctttgg
    13621 aataggtcag cataatgttt gcgccccatt tcccctcggg ctggtcgtat ccgaacccca
    13681 aaacataacg cgacggctgt accgcatcca aagcatagct gcggagggac aagtccggcc
    13741 ggttggatac cgatttcggt ttgatacggt tgtacgccaa tgtggtgtac aggccttcgg
    13801 gcagtttgcc gtatacgccg ttccagtcga ttttccccaa gatgttgatg ccttgaagcg
    13861 acatattttg ggcattgtaa taatcgcgta tatcaatctc tgtcaatcgt cctgctgaat
    13921 ccggcaattt ggttttttga tcggcaacgg caatcatatc ggtataacgg ttgcggaaac
    13981 tgctgatttc caaaaagccg aaatcgccct tccactgcaa accgatttcc cggttggctg
    14041 ccttttccga tttcagggcg ggacgctgcc agcctttcgg ataatcgtga taaatgtcta
    14101 tcccgaaaag ttcttggaag gaaggcgttc tgaagccgct ggaggcgcgg taagacacgg
    14161 aaaaatgccg gttcggtttg aacacgatgc cgctgttcca cgaacggtcg gcataccgcc
    14221 cgctgcggac gagttcttcc gacgtggtga agtttttccg gtcgtacctg ccgcccaagc
    14281 tgaaatcaaa atatttgccg attgaaaaac ggtcgttcaa agaaatgtgg atattgctgc
    14341 cgttgatttt tcttggcacg catttgcggg aacgcagggt ttcgatatag ccgcagaccg
    14401 acccttcgac gacttcgggc ttacccaaac ggtacttatc ttgattcttt tcatcgaatc
    14461 ccgtggattc cgaaatcctt gccgcattgt gggaaagctg ttctgggcgg gagatcgctt
    14521 tggaagcatc gtaaccgaag cccaaagtca gatggtgttt cgtccatttg tttttcagcg
    14581 atttctcaaa cgaggcattc aaaacattgt gctgttcgcg gtagtggaaa cggtcgctgc
    14641 tgtcgtagga atacggtttg tccgccgacg cgcggcagga tttgtccacg gcaggataca
    14701 cggcgcaatt cagcttcagc gtgttgttgt cggttgccac gccctgtttg tcaaacgaca
    14761 acaccgcctt atccgcccag ttgtccgagt atttctcatt ttcataacgg tacagcaaac
    14821 ccatacggcg gcggcggtga tgttcgtcaa taaatttggt gcgggaatat ttcaaaccta
    14881 tgcccttgac caaattttta tcgcccttcc actcttctat attcggcaca aaatacaagc
    14941 cgtcgcggaa atcgtcgccg tcgtacaccc cgctcttgtc tctaaacttt tccgcctcgt
    15001 ccgtaccgta atactgtttt tccgtcatat cgcggatgtc gtaacgctgt ttggtatcct
    15061 caaacacgcc gccgacataa tgcctgccgc cgaagcggta gcccagcttg gcaagccaag
    15121 agccgctgcg gtaatccatc ggatcgggca atatcctgcc gccgcccgtg taggcttggg
    15181 cggacaggtt ttcgtggcgc gcctgcgcct cccgcacctg cgcctcttct tcagcactta
    15241 aaggctgatt ttgttcaata cgttctttta cccagcggtt gagctggcgg ttcaaatata
    15301 tcccgtagcc cgccaatttt gccacgggct tgagtgtatt ctcgccctct actaagaaaa
    15361 atggctctgt tgtcttgcgt ttaatatcgt atgtctggcg gaacgcgtcc aaacggtcta
    15421 tgccgtattc caccccgtcc gcaatatcgc cgtgcgggcg cgtttcccgc ccttggcgtt
    15481 cggttcggat tagcagccct tcccaaccgt ctttgctgaa ccccgcgccg agcgacttca
    15541 taaattggcg gtttttactg ccgtaagcgg tttttgcctg tatcccccgg cttttgccgt
    15601 ctgaaatcag gtctgccgcc tctttggtgc ggaaggcgac cgcgccgccg agtgcgccgc
    15661 tgccgtgatc ggacgaaccg gcgcctttgt cgatttccac cgtgctgatg ttttcatatt
    15721 cgatttcgtt gattgcgccg ctgccgccgc gtccgccgta tccgctcaac gatccctgca
    15781 cggtaaacgc ctgtatttgg gcaacgccgt caaccgaaac cgccacacgg tttttatcta
    15841 cgccgcgtat cgagtagccg ccgctcgcgc cgttgccctg ttcgacaacc gccacgcccg
    15901 gatcgtagcg cgtcaggtcg cggataccga gtacctgttc tttgttcaac gtttccgacg
    15961 ttttgacgat tttgcccaaa cctgtcgcct ctttcgatcg ccgtcccact ttggcggcac
    16021 ggacggtaac ctctttcaat gattgacttt gcgccgcatc aagtgccgcc gcccccgctt
    16081 gggcagcata agccggaaaa gcggttgcaa tggccaacgc cgtcaaagtc agcggaaaac
    16141 cgtgtttctt attcattttt ccacctcttt attatctttc ttcgcaccga ataccacgcc
    16201 gaattggggt ttaacttcag gttctaactg ttcgccaaca tcagcttcat tttcaacttc
    16261 attttcaata tcttcagtta taccaagaga tttcccatcc ttattgaaaa tagtgccgcc
    16321 caattccgcc gcctgcgggc cgtaaaagcc gcccgttaca agaagattgt cggctttgaa
    16381 actttgagga ttagtcgaac catttcccga aagattgatg ccgttatccc gagtgcgtgc
    16441 tgttgcgtgg aaaccgttgc cctcaatcac gccgttttca atacggaaag caggttctac
    16501 accgttttgc tccgtcagcg ttccggaaat cgatttattg ccgaaatcaa cgtcaaattc
    16561 tgcttttgcc gctttatccg ccttattatc ccattgaatg ggttcgccga tacgcgcttc
    16621 ccaagtgccg gtataatgcg ccgttccggt ttttggaatg tcggcttccg ccgtgcggat
    16681 acctttcagg aaaaggtcga tgtccctgcc tttaagggct tccggggcgg gcgggatgcc
    16741 gtctgaaaca ccgttgcctt cttccggcga ttcttcttcg ggttcttcct cttcagcttc
    16801 ttcttcgatt tcttcagctt catcagcttc ttcttcgacg atttcgtctt cgtcttcgcc
    16861 gttatcatct tcggaaactt cgtcttcgct ttcttcggcg atttcttcgt cttcgctttc
    16921 ttcaccgtta ttaatacccg aatcctcttc gtcctgcgcc ttcggtttga cggcggggcg
    16981 ttcggttttt atccgtccga gtttcacata ggtcaaaaag tcgcaacaag cactgacggt
    17041 cattttcctg ccgtcggcaa gatcgatggt tttctctttg ctaaccaaag gaatttcacg
    17101 cccttcgaca agaagtttgt caggatgacc aaaatcgggc atagtggaaa cctcaaacgg
    17161 acggggattt tcatcagtcg cctcgtcaac ggaaattttt agagaatcca agattttggt
    17221 gtgttttcca aacggcatag caggatttgt gcctgatgcg tttgtttttt gtttgcctgc
    17281 gaatacgccg aatacgccgt tgtcgttact gataaaccgt ccggcaagct cttctccgtt
    17341 atcgccgaaa aaaccgccct caagccgctg atcggcatcg gtatggaaaa acaaatattc
    17401 tttatcagcg tgtctcgtct tcacctcggt gttaactttg gcactgccgg taaagcggtt
    17461 gccgtttaaa tcggcagtga tgttgtaaat ggtcagcggt tttttaggtt cctttggatc
    17521 atttctcttt tgcacatact gatttttaat cagctcgccc tttaggattt ttttgtcgaa
    17581 atcaaccgta tattcggcag gatgtttttc cctgtcgtcg gcatccttag ccgcataaga
    17641 agttgcacca atatcgttac cataaaatgt tatataaccc aaatcgtcac tggcaaccgc
    17701 cttacctgtc cgatgacgtt tggcatcggt catatattgc cagttgccga aatattttac
    17761 cgttcccgcg ctcggtaaag attgggaagg acgttctccg gaataatata caaaaccgtc
    17821 ataaccaaaa cggtgggtaa accgcttacc acctgagttc tgttcgattt catcttttcc
    17881 gtttttagta tatacataac cggcatccac aaatttataa ttatattttt tattttcctt
    17941 ttctgacgtc ctaatttcta catcggaatc acgtttatga atttcgcttt taagttgttg
    18001 aagtttattt ccttttgaac cgtataaaaa cagaacatca ccttctttaa acgacagctt
    18061 ttcttctgct tgtttgctat cgggaattgc cgtgccatct tcacgatgaa aagcagtatt
    18121 ccgcctcaac aaccgcattg ccgcaccgac ggc
//
LOCUS       DAWTQS010000046        17787 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.046, whole genome shotgun sequence.
ACCESSION   DAWTQS010000046 DAWTQS010000000
VERSION     DAWTQS010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17787
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.046"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            17..328
                     /gene="rpsJ"
                     /locus_tag="WNA25_001452"
     CDS             17..328
                     /gene="rpsJ"
                     /locus_tag="WNA25_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFA9824184.1"
                     /translation="MANQKIRIRLKAYDYALIDRSAQEIVETAKRTGAVVKGPIPLPT
                     KIERFNILRSPHMNKTSREQLEIRTHLRLMDIVDWTDKTTDALMKLDLPAGVDVEIKV
                     Q"
     gene            complement(414..596)
                     /locus_tag="WNA25_001453"
     CDS             complement(414..596)
                     /locus_tag="WNA25_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824185.1"
                     /translation="MDASAQTKPDSTFFRGASLRKVMDKKYPVLFTKKCRLKILEAQN
                     KPISPTALFFDFVVLP"
     gene            complement(670..783)
                     /locus_tag="WNA25_001454"
                     /pseudo
     CDS             complement(670..783)
                     /locus_tag="WNA25_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            1114..1758
                     /gene="rplC"
                     /locus_tag="WNA25_001455"
     CDS             1114..1758
                     /gene="rplC"
                     /locus_tag="WNA25_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFA9824186.1"
                     /translation="MTLGLVGRKVGMTRVFDEQGVSVPVTVLDMSANRVTQVKSKDAD
                     GYTAVQVTFGQKKANRVNKAEAGHFAKAGVEAGRGLIEFALTEEKLAELKAGDEITVS
                     MFEVGQLVDVTGTSKGKGFSGTIKRHNFGAQRTSHGNSRSHRVPGSIGMAQDPGRVFP
                     GKRMAGQYGNTKATVQKLEVVRVDVERQLLLVKGAVPGAVNSDVVVRPSVKVGA"
     gene            1758..2378
                     /gene="rplD"
                     /locus_tag="WNA25_001456"
     CDS             1758..2378
                     /gene="rplD"
                     /locus_tag="WNA25_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFA9824187.1"
                     /translation="MELKVIDAKGQVSGSLSVSDALFAREYNEALVHQLVNAYLANAR
                     SGNRAQKTRAEVKHSTKKPWRQKGTGRARSGMTSSPLWRKGGRAFPNKPDENFTQKVN
                     RKMYRAGMATILSQLARDERLFAIEALTAETPKTKVFAEQVKNLGLEQVLFVTKQLDE
                     NVYLASRNLPNVLVLEAQQVDPYSLLRYKKVIITKDAVAQLEEQWV"
     gene            2375..2689
                     /gene="rplW"
                     /locus_tag="WNA25_001457"
     CDS             2375..2689
                     /gene="rplW"
                     /locus_tag="WNA25_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFA9824188.1"
                     /translation="MNQQRLTQVILAPIVSEKSNVLAEKRNQMTFKVLANATKPEIKA
                     AVELLFGVQVASVTTVTTKGKTKRFGRTLGRRSDVKKAYVSLAAGQELDLEAAAAAAD
                     KE"
     gene            2695..3528
                     /gene="rplB"
                     /locus_tag="WNA25_001458"
     CDS             2695..3528
                     /gene="rplB"
                     /locus_tag="WNA25_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFA9824189.1"
                     /translation="MAIVKMKPTSAGRRGMVRVVTEGLHKGAPYAPLLEKKNSTAGRN
                     HNGHITTRHKGGGHKHHYRVVDFKRNKDGIPAKVERIEYDPNRTAFIALLCYADGERR
                     YIIAPRGIQAGAVLVSGAEAAIKVGNTLPIRNIPVGTTIHCIEMKPGKGAQIARSAGA
                     SAVLLAKEGAYAQVRLRSGEVRKINVDCRATIGEVGNEEQSLKKIGKAGANRWRGIRP
                     TVRGVVMNPVDHPHGGGEGRTGEAREPVSPWGTPAKGYRTRNNKRTDNMIVRRRYSNK
                     G"
     gene            3534..3812
                     /gene="rpsS"
                     /locus_tag="WNA25_001459"
     CDS             3534..3812
                     /gene="rpsS"
                     /locus_tag="WNA25_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFA9824190.1"
                     /translation="MARSLKKGPYVDLHLLKKVDAVRASNDKRPIKTWSRRSTILPDF
                     IGLTIAVHNGRTHVPVFISDNMVGHKLGEFSLTRTFKGHLADKKAKKK"
     gene            3821..4150
                     /gene="rplV"
                     /locus_tag="WNA25_001460"
     CDS             3821..4150
                     /gene="rplV"
                     /locus_tag="WNA25_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFA9824191.1"
                     /translation="MRVNAQHKNARISAQKARLVADLIRGKDVAQALNILAFSPKKGA
                     ELIKKVLESAIANAEHNNGADIDELKVVTIFVDKGPSLKRFQARAKGRGNRIEKQTCH
                     INVTVGN"
     gene            4160..4852
                     /gene="rpsC"
                     /locus_tag="WNA25_001461"
     CDS             4160..4852
                     /gene="rpsC"
                     /locus_tag="WNA25_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFA9824192.1"
                     /translation="MGQKINPTGFRLAVTKDWASKWFAKSTDFSTVLKQDIDVRNYLR
                     QKLANASVGRVIIERPAKSARITIHSARPGVVIGKKGEDIEVLKRDLQVLMGVPIHVN
                     IEEIRRPELDAQIIADGIAQQLEKRVQFRRAMKRAMQNAMRSGAKGIKIMTSGRLNGA
                     DIARSEWYREGRVPLHTLRANVDYATSEAHTTYGVLGLKVWVYTEGNIKSSKPEHESK
                     QRKAGRRNAAAN"
     gene            4836..5252
                     /gene="rplP"
                     /locus_tag="WNA25_001462"
     CDS             4836..5252
                     /gene="rplP"
                     /locus_tag="WNA25_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFA9824193.1"
                     /translation="MLQPTRLKYRKQQKGRNTGIATRGNKVSFGEFGLKAVGRGRLTA
                     RQIEAARRAMTRHIKRGGRIWIRVFPDKPITEKPIQVRMGGGKGNVEYYIAEIKPGKV
                     LYEMDGVPEELAREAFELAAAKLPIPTTFVVRQVGQ"
     gene            5252..5443
                     /gene="rpmC"
                     /locus_tag="WNA25_001463"
     CDS             5252..5443
                     /gene="rpmC"
                     /locus_tag="WNA25_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFA9824194.1"
                     /translation="MKANELKDKSVEQLNADLLDLLKAQFGLRMQNATGQLGKPSELK
                     RVRRDIARIKTVLTEKGAK"
     gene            5443..5706
                     /gene="rpsQ"
                     /locus_tag="WNA25_001464"
     CDS             5443..5706
                     /gene="rpsQ"
                     /locus_tag="WNA25_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFA9824195.1"
                     /translation="MSETKNVRTLQGKVVSDKMDKTVTVLVERKVKHSLYGKIIRLST
                     KIHAHDENNQYGIGDVVVISESRPLSKTKSWVVSELVEKARSI"
     gene            5928..6296
                     /gene="rplN"
                     /locus_tag="WNA25_001465"
     CDS             5928..6296
                     /gene="rplN"
                     /locus_tag="WNA25_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFA9824196.1"
                     /translation="MIQMQTILDVADNSGARRVMCIKVLGGSKRRYASVGDIIKVAVK
                     DAVPRGRVKKGDVYNAVVVRTAKGVRRPDGALIKFDNNAAVLLNNKLEPLGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            6308..6631
                     /gene="rplX"
                     /locus_tag="WNA25_001466"
     CDS             6308..6631
                     /gene="rplX"
                     /locus_tag="WNA25_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFA9824197.1"
                     /translation="MNKIIKGDRVVVIAGKDKGKQGQVVRVLGGKVVVEGVNVVKRHQ
                     KPNPMRGIKGGIITKEMPLDISNIAILNPETNKADRVGIKLIENEGKVKRVRFFKSNG
                     SIIGA"
     gene            6641..7180
                     /gene="rplE"
                     /locus_tag="WNA25_001467"
     CDS             6641..7180
                     /gene="rplE"
                     /locus_tag="WNA25_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFA9824198.1"
                     /translation="MARLREFYKETVVPELVKQFGYKSVMEVPRIEKITLNMGVGEAV
                     ADKKVMEHAVSDLERIAGQRPVVTVARKSIAGFKIRDNYPVGCKVTLRRDQMFEFLDR
                     LITIALPRVRDFRGVSGKSFDGRGNYNMGVREQIIFPEIEYDKIDALRGLNITITTTA
                     KTDEEAKALLSLFKFPFKG"
     gene            7183..7488
                     /gene="rpsN"
                     /locus_tag="WNA25_001468"
     CDS             7183..7488
                     /gene="rpsN"
                     /locus_tag="WNA25_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFA9824199.1"
                     /translation="MAKKALINRDLKRQALAKKYAAKRAAIKAVINDSNATEEERFEA
                     RLRFQSIPRNAAPVRQRRRCALTGRPRGTFRKFGLGRIKIREIAMRGEIPGVVKASW"
     gene            7504..7896
                     /gene="rpsH"
                     /locus_tag="WNA25_001469"
     CDS             7504..7896
                     /gene="rpsH"
                     /locus_tag="WNA25_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFA9824200.1"
                     /translation="MSMHDPISDMLTRIRNAQRANKAAVAMPSSKLKCAIAKVLKEEG
                     YIEDFAVSSDVKSILEIQLKYYAGRPVIEQIKRVSRPGLRIYKASSEIPSVMNGLGIA
                     IVSTSKGVMTDRKARSQGVGGELLCIVA"
     gene            7910..8443
                     /gene="rplF"
                     /locus_tag="WNA25_001470"
     CDS             7910..8443
                     /gene="rplF"
                     /locus_tag="WNA25_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFA9824201.1"
                     /translation="MSRVAKNPVTVPAGVEVKFGTEALVIKGKNGELSFPLHSDVAIE
                     FNDGKLTFVANNSSKQANAMSGTARALVSNMVKGVSEGFEKKLQLIGVGYRAQAQGKI
                     LNLSLGFSHPIVYEMPEGVSVQTPSQTEIVLTGSDKQVVGQVASEIRAFRAPEPYKGK
                     GVRYVGEVVVMKEAKKK"
     gene            8457..8810
                     /gene="rplR"
                     /locus_tag="WNA25_001471"
     CDS             8457..8810
                     /gene="rplR"
                     /locus_tag="WNA25_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFA9824202.1"
                     /translation="MDKHTTRLRRARKTRARIADLKMVRLCVFRSNNHIYAQVISAEG
                     DKVLAQASTLEAEVRGSLKSGSNVEAAAIVGKRIAEKAKAAGVEKVAFDRSGFQYHGR
                     VKALAEAARENGLSF"
     gene            8829..9347
                     /gene="rpsE"
                     /locus_tag="WNA25_001472"
     CDS             8829..9347
                     /gene="rpsE"
                     /locus_tag="WNA25_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFA9824203.1"
                     /translation="MAKHEIEERGDGLIEKMVAVNRVTKVVKGGRIMAFSALTVVGDG
                     DGRIGMGKGKSKEVPVAVQKAMDQARRSMIKVPLKNGTIHHEVIGRHGATKVFMQPAK
                     EGSGVKAGGPMRLVFDAMGIHNISAKVHGSTNPYNIVRATLDGLSKLHTPADIAAKRG
                     LTVEDILGVNHG"
     gene            9340..9525
                     /gene="rpmD"
                     /locus_tag="WNA25_001473"
     CDS             9340..9525
                     /gene="rpmD"
                     /locus_tag="WNA25_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFA9824204.1"
                     /translation="MAEQKKIRVTLVKSLIGTIESHRACARGLGLRRREHTVEVLDTS
                     ENRGMINKISYLLKVES"
     gene            9527..9961
                     /gene="rplO"
                     /locus_tag="WNA25_001474"
     CDS             9527..9961
                     /gene="rplO"
                     /locus_tag="WNA25_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFA9824205.1"
                     /translation="MFLNTIQPAVGATHAGRRVGRGIGSGLGKTGGRGHKGQKSRSGG
                     FHKVGFEGGQMPLQRRLPKRGFKSLTVSANAQLRLSELESIAVNEIDILVLKQAGLIA
                     STVSNVKVIASGEISKAVALKGIKVTKGARAAIEDVGGKIEM"
     gene            9973..11283
                     /gene="secY"
                     /locus_tag="WNA25_001475"
     CDS             9973..11283
                     /gene="secY"
                     /locus_tag="WNA25_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFA9824206.1"
                     /translation="MANQQTSSGSSKFGDLKKRLLFLFGALIVFRIGAHIPVPGVDAV
                     ALAKLYESAGNGILGILNMFSGGSLERFSIFAIGIMPYISASIIVQLASEILPSLKAL
                     KKEGEAGRKVITKYTRYGTVLLAILQSLGVASFVFQQGIVVTSSFEFHVSTVVSLVTG
                     TMFLMWLGEQITERGIGNGISLIITAGIASGIPSGIAKLVTLTNQGSMSMLTALLIVF
                     GALLLIYLVVYFESAQRKIPIHYAKRQFNGRVGSQNTHMPFKLNMAGVIPPIFASSII
                     LFPSTLLGWFGSADTNSVLHKIAGLLQHGQLLYMALFAATVIFFCYFYTALVFSPKEM
                     AENLKKSGAFVPGIRPGEQTSRYLEKVVLRLTLFGALYITTICLIPEFLTTVLNVPFY
                     LGGTSLLILVVVTMDFSTQINSYRLTQQYDKLMTRSEMKSFSRK"
     gene            11288..11506
                     /gene="infA"
                     /locus_tag="WNA25_001476"
     CDS             11288..11506
                     /gene="infA"
                     /locus_tag="WNA25_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFA9824207.1"
                     /translation="MAKEDTIQMQGEILETLPNATFKVKLENDHIVLGHISGKMRMHY
                     IRISPGDKVTVELTPYDLTRARIVFRAR"
     gene            11527..11640
                     /gene="rpmJ"
                     /locus_tag="WNA25_001477"
     CDS             11527..11640
                     /gene="rpmJ"
                     /locus_tag="WNA25_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFA9824208.1"
                     /translation="MRVQPSVKKICRNCKIIRRNRVVRVICTDPRHKQRQG"
     gene            11706..12068
                     /gene="rpsM"
                     /locus_tag="WNA25_001478"
     CDS             11706..12068
                     /gene="rpsM"
                     /locus_tag="WNA25_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFA9824209.1"
                     /translation="MARIAGVNIPNNAHIVIGLQAIYGIGATRAKLICEAANIAPDTK
                     AKDLDETQLDALRDQVAKYEVEGDLRREVTMSIKRLMDMGCYRGFRHRRGLPCRGQRT
                     RTNARTRKGPRKAIAGKK"
     gene            12088..12483
                     /gene="rpsK"
                     /locus_tag="WNA25_001479"
     CDS             12088..12483
                     /gene="rpsK"
                     /locus_tag="WNA25_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFA9824210.1"
                     /translation="MAKANTASRVRKKVRKTVSEGIVHVHASFNNTIITITDRQGNAL
                     SWATSGGAGFKGSRKSTPFAAQVAAEAAGKVAQEYGVKNLEVRIKGPGPGRESSVRAL
                     NALGFKITSITDVTPLPHNGCRPPKKRRI"
     gene            12503..13123
                     /gene="rpsD"
                     /locus_tag="WNA25_001480"
     CDS             12503..13123
                     /gene="rpsD"
                     /locus_tag="WNA25_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFA9824211.1"
                     /translation="MARYIGPKCKLARREGTDLFLKSARRSLDSKCKIDSAPGQHGAK
                     KPRLSDYGLQLREKQKIRRIYGVLERQFRRYFAEADRRKGSTGELLLQLLESRLDNVV
                     YRMGFGSTRAEARQLVSHKAIVVNGQVVNIPSFQVKAGDVVSVREKAKKQVRIQEALG
                     LATQIGLPGWVSVDADKLEGVFKNMPDRSELTGDINEQLVVEFYSK"
     gene            13149..14135
                     /gene="rpoA"
                     /locus_tag="WNA25_001481"
     CDS             13149..14135
                     /gene="rpoA"
                     /locus_tag="WNA25_001481"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFA9824212.1"
                     /translation="MQNSTTEFLKPRQIDVNTFSATRAKVSMQPFERGFGHTLGNALR
                     RILLSSMNGFAPTEVAIAGVLHEYSTVGGVQEDVVDILLNIKGIVFKLHGRSQVQLVL
                     KKSGSGVVSAGDIELPHDVEILNPGHVICHLADNGQIEMEIKVEQGRGYQSVSGRQVV
                     RDENRQIGAIQLDASFSPISRVSFEVEPARVEQRTDLDKLVLDIETDGSIDPEEAVRS
                     AARILIDQMSIFADLQGTPVEEVEEKSPPIDPVLLHPVDDLELTVRSANCLKAEDIYY
                     IGDLIQRTETELLKTPNLGRKSLNEIKEVLASKGLTLGSKLEAWPPVGLEKP"
     gene            14159..14527
                     /gene="rplQ"
                     /locus_tag="WNA25_001482"
     CDS             14159..14527
                     /gene="rplQ"
                     /locus_tag="WNA25_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFA9824213.1"
                     /translation="MRHRNGNRKLNRTSSHRAAMLRNMANSLLTHETIVTTLPKAKEL
                     RRVVEPLITLGKKPSLASRRLAFDRTRDRDVVVKLFGDLGPRFTARNGGYVRVLKYGF
                     RKGDNAPLALVELVDKPAAE"
     gene            14674..15387
                     /gene="minC"
                     /locus_tag="WNA25_001483"
     CDS             14674..15387
                     /gene="minC"
                     /locus_tag="WNA25_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFA9824214.1"
                     /translation="MMVYIMNAFDIKSTKMDVLSISLHTSDLFDLEDVLVKLGKKFQE
                     SGVVPFVLDVQEFDYPESLDLAALVSLFSRHGMQILGLKHSNERWAAVAMKYHLLFCL
                     SHSENVKELGQVEVQKTEDGQKARKTVLITSPVRTGQQVYAEDGDLIVTGAVNQGAEL
                     IADGNMHIYAPMRGRALAGAKGDTSARIFIHSMQAELVSVAGIYRNFEQDLPDHLHKQ
                     PVQILLQDNRLVISAIGSE"
     gene            15416..16231
                     /gene="minD"
                     /locus_tag="WNA25_001484"
     CDS             15416..16231
                     /gene="minD"
                     /locus_tag="WNA25_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFA9824215.1"
                     /translation="MAKIIVVTSGKGGVGKTTTSASIATGLALRGYKTAVIDFDVGLR
                     NLDLIMGCERRVVYDLINVIQGEATLNQALIKDKNCENLFILPASQTRDKDALTREGV
                     EKVMQELSGKKMGFEYIICDSPAGIEQGALMALYFADEAIVTTNPEVSSVRDSDRILG
                     ILQSKSRKAEQGGSVKEHLLITRYSPERVAKGEMLSVQDICDILRIPLLGVIPESQNV
                     LQASNSGEPVIHQDSVAASEAYKDVIARLLGENREMRFLEAEKKSFFKRLFGG"
     gene            16235..16498
                     /gene="minE"
                     /locus_tag="WNA25_001485"
     CDS             16235..16498
                     /gene="minE"
                     /locus_tag="WNA25_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFA9824216.1"
                     /translation="MSLIELLFGRKQKTATVARDRLQIIIAQERAQEGQTPDYLPTLR
                     KELMEVLSKYVNVSLDNIRISQEKQDGMDVLELNITLPEQKKV"
     gene            16502..17422
                     /gene="oxyR"
                     /locus_tag="WNA25_001486"
     CDS             16502..17422
                     /gene="oxyR"
                     /locus_tag="WNA25_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator OxyR"
                     /protein_id="HFA9824217.1"
                     /translation="MTLTELRYIVAVAQERHFGRAARRCFVSQPTLSIAIKKLEEELA
                     VSLFDRSSNDIITTEAGERIVAQARKVLKEAELIRHLANEEQNELEGAFKLGLIFTVA
                     PYLLPKLIVSLRRTAPKMPLMLEENYTHTLTESLKRGDVDAIIVAEPFQEPGIVTEPL
                     YDEPFFVIVPKGHSFEELDAVSPRMLGEEQVLLLTEGNCMRDQVLSSCSELAAKQRIQ
                     GLTNTLQGSSINTIRHMVASGLAISVLPATALTENDHMLFSIIPFEGTPPSRRVVLAY
                     RRNFVRPKALSAMKAAIMQSQLHGVSFIHD"
BASE COUNT         4905 a         3342 c         4387 g         5153 t
ORIGIN      
        1 tgaaggatat tgataaatgg caaaccaaaa aatccgtatc cgcctgaaag cttatgatta
       61 cgccctgatt gaccgttctg cacaagaaat cgttgaaact gcaaaacgta ccggtgctgt
      121 tgtaaaaggc ccgattcctt tgccgaccaa aatcgagcgt ttcaacattt tgcgttctcc
      181 gcacatgaac aaaacttccc gtgaacaatt ggaaatccgc acccatttgc gcctgatgga
      241 catcgtggat tggaccgata aaactaccga tgcgctgatg aagctggatt tgccggccgg
      301 tgttgatgta gaaatcaaag tccaataatt cggactgtcg aaaatcccca agcaatcaat
      361 gcttggggat tttttatgtt atgccgtctg aaaatatgcg gcggcaaaca gatttaaggc
      421 aaaacaacaa aatcaaaaaa cagtgcggtc ggtgaaatcg gcttattttg tgcctcaaga
      481 attttcagac ggcatttttt agtgaacaaa acaggatatt ttttatccat aactttccta
      541 aggcttgccc cccgaaaaaa ggtagaatcg ggcttggttt gggcagaagc atccatctga
      601 ttctaaggat ggatgatgtt ttattctcaa gtaaataaag gaaagatgat gagcctacta
      661 atgtctttaa agatatagtg gattaaattt aaaccggtac ggcgttgcct cgccttgccg
      721 tactatttgt actgtctgcg gcttcgtcgc tttgtcctga tttaaattta atccactata
      781 ttttaaacaa caaagaattg aatttggaag aacggcatga tttggagcgg gcggttaaga
      841 tcttgcagga cacgcacgtc atcccggttt gtcggagaaa gatgcatacg ccgtgatggt
      901 cggataccga cactttaaaa gcatccgacc ccatgccttc tgaagattca aaacgcttca
      961 gacggcatat tgaagatatt tctgatattt ctgttgatat ttctttgact tgtcggatat
     1021 aatgtctgac ttggtacatt cgtgccaagt ttaactttgt ctgaaagaca ggccaatcgt
     1081 agcctgtccc tttactttaa aaggaaaata atcatgactt taggtctggt tgggcgcaaa
     1141 gttggtatga cccgcgtgtt cgacgaacaa ggtgtttctg ttccggtaac cgttttggat
     1201 atgtctgcca accgcgttac acaagtaaaa tccaaagatg ctgacggcta tactgccgtt
     1261 caagttacct ttggtcagaa aaaagctaac cgtgtcaata aagccgaagc cggacacttt
     1321 gcaaaagcag gtgttgaagc cggtcgcggt ttgattgagt ttgctttgac tgaagaaaaa
     1381 ctggctgaat tgaaggccgg tgacgaaatt accgtttcta tgtttgaagt cggtcaactg
     1441 gttgatgtaa ccggtacctc taaaggtaaa ggtttctccg gtacgatcaa acgtcataac
     1501 ttcggtgctc aacgtacttc ccacggtaac tcccgttctc accgtgttcc aggctctatc
     1561 ggtatggcgc aagacccggg tcgcgtgttc cctggtaaac gcatggccgg tcaatacggt
     1621 aacaccaaag caactgtcca aaaactggaa gttgtacgtg ttgatgtaga acgccaactg
     1681 ctgttggtca agggtgctgt tccgggtgcg gtcaacagcg atgttgtagt tcgtcctagc
     1741 gtgaaagtag gtgcgtaatg gaattgaaag taattgacgc taaaggacaa gtttcaggca
     1801 gtctgtctgt ttctgatgct ttgttcgctc gcgaatacaa tgaagcgttg gttcatcagc
     1861 tggtaaatgc ctacctggca aacgcccgct ctggtaaccg cgctcaaaaa acccgtgccg
     1921 aagtaaaaca ctcaaccaaa aaaccatggc gtcaaaaagg taccggccgt gcacgttccg
     1981 gtatgacttc ttctccgctg tggcgtaaag gtggtcgcgc gttcccgaac aaacctgacg
     2041 aaaacttcac tcaaaaagta aaccgtaaaa tgtaccgtgc tggtatggcg actattctgt
     2101 cccaattggc tcgtgacgag cgtttgtttg ctatcgaagc gttgactgct gaaactccta
     2161 aaaccaaagt ttttgctgaa caagtgaaaa atctgggtct ggagcaagtg ttgtttgtaa
     2221 ccaaacagct cgacgagaat gtttacttgg cttcacgcaa cttgccaaac gtgttggttt
     2281 tggaagctca acaagttgat ccttacagct tgctgcgtta caaaaaagta atcatcacta
     2341 aagatgcagt tgcacaatta gaggagcaat gggtatgaat caacaacgtt tgactcaagt
     2401 gattttggca cctatcgttt ctgaaaaaag caacgtattg gctgaaaaac gcaaccaaat
     2461 gacgtttaaa gtcttggcaa atgcaaccaa acctgaaatc aaagctgctg ttgagctgct
     2521 gtttggtgtt caagttgctt ctgtaactac cgttacaact aaaggcaaaa ctaagcgttt
     2581 tggtcgtact ttgggccgcc gcagcgatgt taaaaaagct tatgtaagct tggcagccgg
     2641 tcaagagttg gatttggaag ccgctgctgc agctgcagat aaggaataaa caaaatggca
     2701 atcgttaaaa tgaagccaac ttctgcaggc cgtcgcggca tggttcgcgt ggtaacagaa
     2761 ggtttgcaca aaggtgcgcc ttatgcacct ttgctcgaaa agaaaaattc tactgccggt
     2821 cgtaaccata atggtcatat caccacccgt cataaaggcg gcggtcataa acaccattac
     2881 cgtgttgtag actttaaacg taacaaagac ggtatccctg caaaagtaga gcgtatcgaa
     2941 tacgatccta accgcactgc ctttattgca ttgttgtgct atgcagacgg cgaacgtcgc
     3001 tacatcatcg ctcctcgcgg tattcaagcc ggtgctgtat tggtttccgg tgctgaagct
     3061 gccatcaaag taggtaacac cctgccgatc cgcaatattc ccgttggtac gactatccac
     3121 tgtatcgaaa tgaaacctgg taaaggtgca caaattgcac gttctgccgg tgcttctgcg
     3181 gtattgttgg ctaaagaagg tgcatacgct caagtccgtc tgcgctctgg cgaagttcgt
     3241 aaaatcaacg tagattgccg tgcgaccatc ggtgaagtcg gtaacgaaga gcaaagtctg
     3301 aaaaaaatcg gtaaagccgg tgctaaccgt tggcgcggta ttcgtccgac cgtacgcggt
     3361 gttgtcatga atcccgtcga tcacccgcat ggtggtggtg aaggccgtac cggcgaagcc
     3421 cgcgaaccgg ttagtccatg gggtactcct gctaaaggct accgcactcg taataacaaa
     3481 cgcacggata atatgattgt tcgtcgccgt tactcaaata aaggttaatt agtatggctc
     3541 gttcattgaa aaaaggccca tatgtagacc tgcatttgct gaaaaaagta gatgctgttc
     3601 gcgcaagcaa cgacaaacgc ccgattaaaa cctggtctcg tcgttctacc attctgcctg
     3661 attttatcgg tctgaccatt gccgtgcaca acggtcgcac ccatgtgcct gtgtttatca
     3721 gcgacaatat ggttggtcat aaattaggcg aattctcatt gacccgtacc tttaaaggcc
     3781 acctggccga taaaaaggct aaaaagaaat aaggtgaatc atgagagtaa atgcacaaca
     3841 taaaaatgcc cgtatctctg ctcaaaaggc tcgtttggta gctgatttga ttcgtggtaa
     3901 agacgttgcc caagctttga atattttggc tttcagccct aaaaaaggtg ccgagctgat
     3961 caaaaaagta ttggagtcag ctattgctaa tgctgagcat aataacggtg cggacattga
     4021 tgaactgaaa gtggtaacta tctttgttga caaaggtcca agcttgaaac gttttcaagc
     4081 tcgcgccaaa ggtcgcggta accgcatcga aaaacaaact tgtcatatca atgtgacagt
     4141 gggtaactaa ggaaaagcta tgggacaaaa gattaaccct acaggctttc gcctggcggt
     4201 aactaaagac tgggcttcaa aatggtttgc taaaagtacc gacttttcta ctgttttgaa
     4261 gcaggatatc gatgttcgca attatttgcg tcaaaaattg gccaatgctt cggttggtcg
     4321 agtaattatt gaacgccctg caaaatctgc acgcattacc attcactccg ctcgtccggg
     4381 tgtggttatc ggtaaaaaag gtgaggatat cgaagttttg aaacgtgact tgcaagtctt
     4441 gatgggtgta cctattcatg taaatattga agagattcgc cgtcctgagt tggatgctca
     4501 aattattgct gatggtatcg cccagcagtt ggaaaaacgc gttcaattcc gtcgtgctat
     4561 gaaacgagca atgcaaaatg caatgcgttc tggtgctaaa ggcattaaga ttatgacttc
     4621 aggccgtctg aatggtgcgg atattgcccg tagcgaatgg tatcgtgaag gtcgcgtgcc
     4681 actgcatact ttacgtgcaa atgtagatta tgcaaccagc gaagcgcaca ccacatatgg
     4741 tgtattgggt ctgaaagttt gggtttatac ggaaggcaat attaaatctt ccaaacctga
     4801 acatgagagt aaacaaagaa aggcaggtag acgtaatgct gcagccaact agactgaaat
     4861 accgtaagca acaaaagggt cgcaataccg gcatcgctac tcgcggtaat aaggtaagtt
     4921 tcggtgagtt cggcttgaaa gccgtaggtc gtggtcgttt gactgcccgt caaatcgaag
     4981 ctgctcgtcg tgcaatgacc cgtcacatca aacgtggtgg tcgtatttgg attcgtgtat
     5041 tccctgacaa accgattact gaaaagccta ttcaagttcg tatgggtggc ggtaaaggta
     5101 acgtggaata ttacattgcc gaaattaaac caggtaaagt gttgtatgaa atggatggcg
     5161 ttccagagga actggctcgt gaagcattcg agttggctgc tgccaaattg cctattccta
     5221 caacctttgt agtaagacag gtgggtcaat aatgaaagca aatgaattga aagacaaatc
     5281 cgttgagcag ttgaatgcag atttgttgga cttgttgaaa gctcagtttg gcttacgtat
     5341 gcaaaacgct accggtcaat taggcaaacc aagtgaattg aaacgtgtac gtcgcgatat
     5401 tgctcgtatt aaaaccgttt taactgaaaa aggtgctaag taatgagcga aactaaaaat
     5461 gttcgtactt tgcaaggcaa agtagtaagc gacaaaatgg ataaaaccgt aacagtattg
     5521 gttgagcgta aagtaaaaca ttcgctgtat ggtaagatta ttcgattatc tactaaaatc
     5581 catgcccatg atgaaaataa tcaatatgga attggtgatg tggttgttat atcggaatcc
     5641 cgtccattgt caaaaactaa atcttgggtt gtcagtgagc tggttgagaa agcacgttct
     5701 atttaagaat taaagcaacg tgcttggaat gggaaacgaa gtattgcagc aaatttaatt
     5761 tgcgtgtaaa cttcgtttcc tgtctttcag tttcttctgg aagtttcttc cctttcggga
     5821 tccaagactg gtttacttga accgcaaggt ttcatttaat aagcagcggc tttgctgtaa
     5881 gttatctgaa agtggtaaat taagttggtt aatttaaagg taataatatg attcaaatgc
     5941 agaccatctt agatgtggct gataactctg gtgcgcgtcg cgtaatgtgt atcaaggtat
     6001 tgggcggatc taagcgtcgc tacgcttctg ttggcgatat tattaaagtg gcagttaaag
     6061 atgcggttcc gcgtggccgt gtcaaaaaag gcgatgtata taatgcggta gttgttcgta
     6121 ctgctaaggg tgtgcgtcgt cctgatggtg cgttaattaa attcgataac aatgccgccg
     6181 tgttgctgaa taataaactt gaacctttgg gtactcgtat ctttggtccg gtaacccgtg
     6241 aattgcgtac tgagcgattt atgaaaatcg tttcattggc acctgaagta ttataaggaa
     6301 tagcacgatg aataaaatca ttaaaggcga tagggttgta gtaattgctg gtaaggataa
     6361 aggtaagcag ggtcaagtag ttcgagtgtt gggtggtaaa gttgttgttg agggcgttaa
     6421 tgttgtaaaa cgccatcaaa aacctaatcc aatgcgtggc attaagggcg gtattattac
     6481 taaagaaatg cctttggata tttctaatat cgcaatcctg aatccggaaa ctaataaagc
     6541 ggaccgtgtt ggtattaagc tgattgaaaa tgaaggcaaa gttaaacgcg ttcgtttctt
     6601 caaatcaaat ggctctatca ttggagcata aggagataac atggctcggt tgagagagtt
     6661 ttataaagag acagttgttc ctgaattggt taaacaattt ggttacaaat cagtaatgga
     6721 agtcccgcgt attgaaaaaa tcaccttgaa tatgggtgtg ggtgaggctg ttgctgacaa
     6781 aaaagttatg gaacatgctg tttccgattt agagagaatt gccggtcaaa gaccggttgt
     6841 tactgttgcc cgtaaatcta tcgcaggttt taaaatccgt gataactatc cggttggttg
     6901 caaagtaaca ttgcgtcgtg atcaaatgtt tgaattcttg gatcgtttga ttactattgc
     6961 attacctcgc gtacgtgact tccgtggtgt gagcggtaaa tcatttgatg gccgtggcaa
     7021 ttacaatatg ggtgttcgtg agcaaattat tttcccggaa attgaatacg ataaaattga
     7081 tgctttgcgt ggtttgaata ttactattac tactacagca aaaaccgatg aggaagcgaa
     7141 agctttattg tcattgttta aatttccgtt caaaggataa tcatggctaa gaaagcactt
     7201 attaatcgtg atctgaaacg tcaagctttg gctaaaaaat atgcggctaa acgcgcggca
     7261 attaaagcgg taatcaatga ttcgaatgca actgaggaag agcgttttga ggctcgtttg
     7321 aggtttcaat ccattcctcg taatgcggca cctgtgcgtc aacgtcgtcg ttgtgctttg
     7381 acaggtcgcc ctcgtggtac tttccgtaaa tttggtttgg gtcgtattaa aatccgtgaa
     7441 atcgccatgc gtggcgaaat tccgggtgtt gttaaagcca gctggtaata ggagtaatta
     7501 agaatgagta tgcatgatcc tatttccgat atgttgactc gtatccgcaa tgcgcaacgt
     7561 gctaataaag cagcagttgc aatgccttct tcaaaattaa agtgtgctat tgcaaaggta
     7621 ttgaaagaag aaggatatat tgaggacttc gcagtttcat ctgacgtaaa gtctatattg
     7681 gaaattcaat taaaatacta tgcaggtcgt cctgtaattg aacaaatcaa gcgtgtatct
     7741 cgccccggtt tgcgtattta taaagcgtct agtgagattc caagtgttat gaatggcttg
     7801 ggtattgcta tcgttagtac ttctaaaggt gtaatgactg atcgtaaagc acgttctcaa
     7861 ggtgttggtg gtgagttgtt atgcattgta gcctagtgga ggaaaagaaa tgtcacgtgt
     7921 cgcaaaaaac ccagtgacgg ttcccgctgg tgtagaagta aaatttggaa cagaggcatt
     7981 ggttattaag ggtaagaacg gtgaattgtc ttttcctttg cattctgatg tagccattga
     8041 atttaatgat ggcaaattga cttttgttgc gaataatagc agtaaacaag caaatgcaat
     8101 gtctggtact gctcgcgcat tagtcagcaa tatggttaaa ggtgtttcag aaggttttga
     8161 gaagaaatta caattgatag gcgtgggtta tcgtgctcaa gcacaaggta aaatcttgaa
     8221 tctgtctttg ggtttttctc atccgatcgt atatgaaatg cctgaaggtg tctccgttca
     8281 aactcctagc caaacagaga ttgttttaac tggctcggat aaacaagttg ttggtcaagt
     8341 tgcttctgag attcgtgcgt tccgtgctcc tgagccttat aaaggtaaag gtgttcgcta
     8401 tgtaggcgaa gtagtggtaa tgaaagaagc caagaaaaaa taattgaggt tcactaatgg
     8461 ataaacatac aacccgactc cgtcgtgcac gcaaaacccg tgctcgtatt gcggacttga
     8521 aaatggtaag attatgtgtg ttccgaagca ataatcatat ttatgctcaa gtaattagtg
     8581 ctgaaggtga taaagtattg gctcaagcct ctacattgga agctgaggtg cgcggtagtc
     8641 tgaaatctgg aagcaatgtt gaagcagctg caatagttgg taaacgtatt gctgagaagg
     8701 ctaaagcagc aggtgtagaa aaggttgctt ttgatcgttc aggtttccaa tatcacggtc
     8761 gtgtgaaggc tttggctgaa gctgctcgtg aaaatggttt aagcttctaa atatttggag
     8821 actttcagat ggcaaaacat gaaattgaag aacgcggtga cggtctgatt gaaaagatgg
     8881 tcgcagttaa ccgtgtaacc aaagtagtta aaggtggtcg cattatggct ttctctgcgc
     8941 taactgttgt tggtgatgga gatggtcgta ttggtatggg taaaggtaag tcaaaagaag
     9001 tgcctgttgc agtccaaaag gcgatggatc aagcacgacg ctctatgatt aaggtaccat
     9061 taaaaaatgg tacgatccat catgaggtta ttggtcgaca tggtgctact aaagtattta
     9121 tgcagcctgc taaagagggt agtggcgtaa aagccggtgg acctatgcgt ttggtttttg
     9181 atgctatggg cattcataat atttccgcca aagtgcacgg atctactaac ccatataata
     9241 tcgtacgtgc aacattagat ggtttgtcta agttgcatac tcctgctgat atcgcagcca
     9301 aacgtggctt gacagtggaa gacattttgg gagttaacca tggctgaaca aaaaaagatt
     9361 agggttacat tggttaaaag cctgattggt acaattgaat ctcatcgtgc atgtgcacgc
     9421 ggtttaggtt tgcgtcgtcg cgagcatacg gtagaggttt tagatacctc tgaaaaccgt
     9481 ggtatgatta ataaaatcag ctacttgttg aaagtggagt cttgatatgt ttttgaatac
     9541 aattcaacct gctgttggtg ctacgcatgc tggtcgtcgt gttggacgcg gtattggtag
     9601 tggtcttggc aaaacgggtg gtcgtggtca taaaggtcaa aagagccggt ctggtgggtt
     9661 tcataaggtg ggtttcgagg gtggtcaaat gcccttgcaa cgacgcctcc ctaaaagagg
     9721 ttttaaatct ttaacagtat cagctaatgc acagcttcgt ttaagtgaac tggaatcaat
     9781 tgctgttaat gagattgata ttttagtctt aaagcaagca ggtctgattg catctacagt
     9841 ctctaatgtt aaagttattg cttctggtga aatttctaag gcagttgctt tgaagggtat
     9901 taaagttacc aaaggtgcga gagctgctat cgaggatgtt ggtggtaaga ttgaaatgta
     9961 aggtttaata ttgtggctaa tcaacaaacg tcatcaggtt catccaagtt tggagatctt
    10021 aagaaacgtc ttttgtttct atttggagca ttgattgttt ttcgaattgg tgcccatata
    10081 cccgtacctg gggttgatgc tgttgcttta gctaaattat acgaaagcgc tggaaacggc
    10141 atcctgggaa tattgaatat gttttccggt gggtcgttag agcgctttag tatatttgca
    10201 ataggaatta tgccatatat ttcagcttct attattgtac agctcgcttc tgaaattttg
    10261 ccatcattga aggctttaaa aaaagaaggg gaggctggta gaaaggtaat tacgaaatat
    10321 actaggtatg gtactgtttt gttagcaatt cttcaaagtc taggtgttgc atctttcgta
    10381 tttcagcaag gaattgttgt aacaagttca tttgagtttc atgtttccac ggtagtttct
    10441 ttggtaacgg gaaccatgtt tcttatgtgg cttggggagc aaattactga aaggggtatc
    10501 gggaacggta tttctttaat cattacggca ggtattgctt caggtattcc ttcgggtatt
    10561 gcaaagctgg ttacactgac gaaccaaggt tctatgagca tgcttacggc gttgcttatt
    10621 gtatttggtg ccttattatt aatttatttg gttgtatact ttgaaagtgc acagcggaag
    10681 attcctattc attatgcaaa acgccagttt aatggtaggg tgggtagtca aaatacgcat
    10741 atgcctttca agttgaatat ggctggtgtt attcccccaa tttttgcttc cagtattatt
    10801 ctatttccat ctactctttt aggttggttt ggttcggctg atacaaatag tgttttgcac
    10861 aaaatagctg gattgttaca acacggtcaa ttgctgtata tggctttatt tgcagcgaca
    10921 gttattttct tttgttattt ttatacggct ttggttttta gtcctaaaga aatggcagag
    10981 aatttaaaaa agagtggtgc ttttgttcct gggattagac ctggtgagca gacctctagg
    11041 tatttagaaa aagttgtatt acgtttgaca ttgtttggag ctctttatat tacaactatt
    11101 tgtttaattc cagagttctt aactacggtt ttaaatgtac ctttttattt gggtggcacg
    11161 tctttgttga ttctagttgt tgtaacgatg gattttagta cacaaataaa ttcgtatagg
    11221 cttactcaac agtatgataa gttaatgact cgttcagaaa tgaaatcatt ttctcggaaa
    11281 tagaattatg gcgaaagaag atactatcca aatgcaaggt gaaattcttg aaactttacc
    11341 taacgcaaca tttaaagtaa aacttgagaa tgaccatatt gtattgggtc atatttctgg
    11401 gaagatgcgg atgcattaca ttcgtatttc tccgggagat aaggtcacag tagagctgac
    11461 accttatgat ttaactaggg ctcgaatcgt tttcagagca agataaacca ataaaaggaa
    11521 aataaaatgc gtgtacaacc atctgttaag aaaatttgcc gaaattgcaa gattattcgt
    11581 cgaaatcgtg tagttcgtgt gatttgtact gatccccgtc acaaacagcg tcaaggttaa
    11641 tggaatattt cttttaatgt gattctgtga tatagtgaca aaatttgctc taaaaaggaa
    11701 aaaacatggc tcgtattgca ggggtaaata tccctaataa cgcacacatc gtaattggtc
    11761 ttcaggctat ttacggtatt ggtgctactc gtgctaaatt gatttgtgag gctgcaaata
    11821 ttgcgcctga tactaaagca aaagatttgg acgagactca attagatgct ttgcgtgatc
    11881 aagttgccaa gtatgaagta gaaggtgatt tgcgtcgtga agtaactatg agtatcaaac
    11941 gattgatgga catgggctgc tatcgtggct tccgtcatcg tcgcggctta ccatgccgcg
    12001 gtcaacgcac tcgtacaaat gcgcgtactc gcaaaggtcc gcgtaaagcg attgctggta
    12061 agaaataaat tttaaggaat tttattaatg gctaaagcaa acacagcttc acgtgtacgt
    12121 aaaaaagtac gtaaaaccgt gagtgagggt attgtgcacg ttcatgcatc tttcaacaat
    12181 accatcatta caatcactga ccgtcaaggc aatgcgttgt cttgggctac ctctggcggc
    12241 gctggtttta aaggttctcg taaaagtaca ccatttgcag cacaagttgc agcagaagca
    12301 gctggtaaag ttgcccaaga gtatggcgtt aaaaatttag aggttcgtat taaaggtcca
    12361 ggtccaggtc gcgaatcctc tgtacgtgct ttgaatgctc ttggtttcaa gattaccagc
    12421 attactgacg ttaccccgtt gcctcataac ggttgccgtc cgcctaaaaa acgtcgtatt
    12481 taatattgga gtgatttgaa acatggcacg ttatattggc cctaaatgta agttggcacg
    12541 tcgcgaaggt acggatttgt ttttgaagag tgcgcgccgc tctttggatt ctaaatgtaa
    12601 aattgattcc gctcccggtc agcatggtgc aaaaaaaccg cgtttgtcag actatggttt
    12661 gcagttgcgt gaaaaacaaa aaatccgccg tatttatggc gtattagaac gtcagttccg
    12721 tcgttatttc gcagaagctg atcgccgtaa aggttctact ggcgagttgc tgttgcagtt
    12781 gctggaatct cgtttggata atgtcgttta tcgtatgggt ttcggttcta cccgagctga
    12841 agcaagacag cttgtttctc ataaggctat agttgtaaat ggacaagttg tcaatattcc
    12901 ttctttccaa gtgaaagctg gtgatgttgt ctcagttcgt gaaaaagcca aaaaacaggt
    12961 acgtattcaa gaagcattgg gtttggcaac tcaaatcggc ttgccgggtt gggtttctgt
    13021 agatgcagat aaacttgagg gtgtgttcaa aaacatgccg gatcgctctg aattgaccgg
    13081 tgatattaat gaacagctgg tggtagagtt ctactctaaa taatgctagc tcagtgaggg
    13141 acagttaaat gcagaatagc acaaccgaat ttttgaaacc tcgtcaaatt gatgtaaata
    13201 ctttttctgc aactcgtgca aaagtatcta tgcagccatt tgaacgtggt ttcggtcata
    13261 ccttaggtaa tgctttgcgc cgtatcttac tgtcatccat gaatggtttt gctcctactg
    13321 aagtagctat tgccggtgta ttgcacgaat attctactgt tggtggtgtt caggaagatg
    13381 ttgttgacat tttgctgaat attaaaggta ttgtgtttaa actccatggt cgtagccaag
    13441 ttcaacttgt gttgaagaaa tcaggttcag gtgtcgtatc tgccggtgat attgagttgc
    13501 cgcatgatgt agaaattctg aatcctggtc atgtcatttg tcatttggct gataacggtc
    13561 aaattgaaat ggaaattaaa gtagagcaag gtcgtggtta tcaatctgtt tcaggtcgtc
    13621 aggtagttcg tgatgagaac cgtcagattg gtgcaatcca gttggatgcg agcttttcgc
    13681 ccatcagccg tgttagcttt gaggttgaac ctgcacgtgt agagcagcgg acggatcttg
    13741 ataaattggt tttggatatc gaaaccgacg gttctattga tcctgaggaa gctgtacgca
    13801 gtgcggcacg tattttgatt gatcagatgt ctatttttgc tgatttgcag ggtactcctg
    13861 tggaggaggt tgaagaaaaa tcacctccta tcgaccctgt tcttttgcat ccggtggatg
    13921 atctggaatt gacagtacgt tcagctaatt gtttgaaagc tgaggatatt tattatattg
    13981 gcgatttgat tcaacgcact gaaaccgagc ttcttaaaac gccgaatttg ggacgtaaat
    14041 ctttgaatga gattaaggaa gtattggcat ctaaaggttt gacactgggt tctaagttgg
    14101 aagcatggcc acctgtaggc ttggaaaagc cttaatgaag aattaaagga taattgatat
    14161 gcgtcatcgt aatggcaatc gcaaattaaa ccgtaccagc agtcatcgtg ctgcaatgct
    14221 gcgtaatatg gcgaattcat tattgactca cgaaactatt gtaacaactc tgcctaaggc
    14281 caaggaattg cgccgtgtag tagagccgtt gattacattg ggtaaaaagc cgtcattggc
    14341 aagccgccgt ttggcatttg accgtactcg cgaccgtgat gttgtagtaa aactgtttgg
    14401 cgatttgggt cctcgtttta ctgctcgtaa cggtggttat gttcgagtgt tgaaatacgg
    14461 attccgtaaa ggtgataatg cacctctggc actggttgaa ttggttgaca aaccggctgc
    14521 tgagtaattt tagtcatata acgccatctg ccgaaaagca ggtggcgtta tttttgcaat
    14581 atctgataga taatatggta ttggctatta tgtttaaaat attaattgaa tagctaaggt
    14641 ttgcgcggta aacttacatc attaaaaaat tctatgatgg tttatataat gaatgccttc
    14701 gatataaagt cgacaaagat ggacgtattg tctatatctt tgcatacatc agacttgttt
    14761 gatttggaag atgtgctggt caaattgggc aagaagtttc aagagtctgg tgttgttcca
    14821 tttgttctgg atgttcaaga atttgattat cccgagtctt tggatcttgc tgcattggtt
    14881 tcgttgtttt caaggcatgg tatgcaaatt ttgggtctga agcattctaa tgaacgttgg
    14941 gctgctgtgg ctatgaagta tcatttgctg ttttgtctgt ctcattcgga aaatgttaaa
    15001 gaactgggtc aggttgaggt gcagaaaacg gaggatggtc agaaagcaag gaaaacagta
    15061 ttgattacat cccctgtccg taccggtcag caggtttatg ccgaagatgg cgatttgatt
    15121 gttacggggg cggtcaacca gggggcggaa ttgattgcag atggcaatat gcatatttat
    15181 gcgccgatga gagggcgtgc tttggccggc gccaaaggtg atacttctgc ccgcatattt
    15241 atccactcca tgcaggcgga gttggtttct gtcgcgggga tttaccgtaa ttttgaacag
    15301 gatttgccgg accatctgca caagcagccg gtacagatat tgttgcagga taaccgattg
    15361 gttatcagtg caattggctc agagtaattg tttgatattt taaaaaggaa atattgtggc
    15421 aaaaattatt gtagtaactt caggcaaggg cggtgtcggt aaaacgacta ccagtgcaag
    15481 tattgcaaca ggtttggcat tacgcggata taaaactgcg gtaattgatt ttgatgtggg
    15541 tttgcgcaac ctcgacctca ttatgggttg cgagcgtcgt gtcgtttatg acctgatcaa
    15601 tgtcattcag ggtgaggcga cgctcaacca ggctttgatt aaagataaaa attgtgaaaa
    15661 cctgtttatt ttgccggctt cccagactcg ggataaagac gctttgacac gcgagggcgt
    15721 agaaaaagtg atgcaggagc tgtccggcaa gaaaatgggc tttgagtata ttatttgcga
    15781 ctctcctgct ggtattgagc agggtgcatt gatggcgttg tattttgctg atgaagccat
    15841 tgtaacgacc aatcctgagg tttccagtgt gcgtgactcc gacaggattt tgggcatttt
    15901 gcaaagcaaa tcccgtaagg cggagcaagg cggttcggtt aaagaacatc tgttaattac
    15961 gcgttattct cccgaacgtg tggcaaaagg cgaaatgctg tctgtacagg atatttgcga
    16021 tattctgcgt attcctttgc tgggtgtgat tcccgaatcc caaaacgtct tgcaggcatc
    16081 caattccgga gaaccggtca tccatcagga cagcgtagcg gcttccgagg catataagga
    16141 cgttattgcc cgtcttttgg gcgagaaccg tgaaatgcgt ttcttggaag ctgagaaaaa
    16201 aagcttcttc aaacgtctgt tcggaggata aggtatgtca ttgatcgaac ttttattcgg
    16261 tagaaagcag aaaacggcaa ccgttgcccg cgaccgcctt caaatcatca ttgcccaaga
    16321 gcgcgcccaa gaaggtcaga ctccggatta cctgccgact ttacgtaaag agttgatgga
    16381 agtcctgtcc aaatatgtga atgtttcatt agacaatatc cgtatttccc aagaaaagca
    16441 ggatggtatg gatgtgcttg agttgaacat tactttgccg gaacagaaaa aggtatagga
    16501 catgacctta accgaattgc ggtacatcgt cgcagtcgcc caagaacgtc atttcggccg
    16561 ggcggcgcgg cgttgttttg tcagccagcc cactttgtct attgccatta agaaattgga
    16621 agaagagctt gccgtctctt tgtttgaccg gagcagcaac gatattatta cgaccgaggc
    16681 gggggaacgt atcgttgcac aggcgcgtaa ggtattgaaa gaggcggagc ttatcaggca
    16741 tttggcaaat gaagaacaaa acgagctgga gggtgcgttc aaactcgggc tgatttttac
    16801 ggttgcgcca tacctgctgc cgaaactgat tgtctctttg cgccgtactg caccgaaaat
    16861 gcctttgatg ttggaagaga attacacgca tactttgacc gagtcgctca aacgcgggga
    16921 cgttgacgcg attatcgttg ccgaaccgtt tcaagagccg ggcattgtta ccgaaccctt
    16981 gtatgacgaa ccgtttttcg tgattgtccc gaaagggcat tcatttgagg aactggatgc
    17041 cgtttcgccc cggatgctgg gtgaggagca ggttttgctg ctgacggaag gcaactgtat
    17101 gcgggatcag gtactctcaa gctgttctga attggcggcg aaacagcgca tacaggggct
    17161 gaccaataca ttgcagggca gctcgatcaa tacaatccgc catatggtcg ccagcggttt
    17221 ggcaatcagc gtgttgccgg caaccgcgct gaccgagaac gatcatatgc tgttcagcat
    17281 tattccgttt gaaggtacgc cgccaagccg gcgggtcgta ttggcgtatc gccgtaattt
    17341 tgtccgtccg aaggcgttgt cggcgatgaa ggcggcgatt atgcagtcgc agcttcacgg
    17401 ggtaagtttt atccacgact aggcgcagaa aatgccgtct gaaacgggtt tcagacggca
    17461 tttgttatct ttgccgtcaa tcaggcaaac ctatccgacc tacagttgct tcgaatcgtc
    17521 attcccgcgc aggcgggaat ctaggtctgt cggtgcggaa acttatcgga taaaacgctt
    17581 gctccaatct cgcgttccag attcccactt tcgtgggaat gacgggatca ggtttccgta
    17641 tggatagatt cgtcattccc gcgcaggcgg gaatctaggt ctgtcggtgc ggaaacttat
    17701 caggtaaaac ggtttctccg gtcccgagtc ctagattccc actttcgtgg gaatgacggc
    17761 atattttgac attgaataaa aaagact
//
LOCUS       DAWTQS010000047        17697 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.047, whole genome shotgun sequence.
ACCESSION   DAWTQS010000047 DAWTQS010000000
VERSION     DAWTQS010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17697
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.047"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            81..1478
                     /gene="aspA"
                     /locus_tag="WNA25_001487"
     CDS             81..1478
                     /gene="aspA"
                     /locus_tag="WNA25_001487"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFA9824218.1"
                     /translation="MTVRIEHDLLGDREIPAEVYWGIHTLRAIENFKISTQKISDVPQ
                     FVRSMVMVKKATAQANGELGAVKPEIAAAIEKACDEVLLNNRCLDQFPSDVYQGGAGT
                     SVNMNTNEVIANLALEALGYEKGRYDIVNPMDHVNASQSTNDAYPTGFRLAVYYSIGE
                     LLDKLTVLKNAFAAKAEAFKDVLKMGRTQLQDAVPMTAGQEFQSFQVLLEEEILNLDR
                     TRQLLLEVNLGATAIGTGVNTPKGYAELVVKKLSEVSGLPCKLTENLIEATSDCGAYV
                     MVHGALKRTAVKLSKICNDLRLLSSGPRAGLKEINLPELQAGSSIMPAKVNPVIPEVV
                     NQVCFKVIGNDTTITFAAESGQLQLNVMEPVIAQCMFETISLLGNAAVNLSDKCVKGI
                     TVNREICERYVFNSIGLVTYLNPYIGHRNGDLVGKICAQTGKGVREVVLERGLLSEEE
                     LNRILSPENLMNPHL"
     gene            complement(1546..3033)
                     /locus_tag="WNA25_001488"
     CDS             complement(1546..3033)
                     /locus_tag="WNA25_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HFA9824219.1"
                     /translation="MNKPLSLALFCLLLPITAHADNPADERRLLDEGSRQTQQYRESG
                     WLDTEQARGEVEENDGYISIGGEIYQVGDTAEELESAIYHALNARQWHKVRQFAARYA
                     KLPRHKPALIHLADALQKRDEGDFRAAGNSFQTALEAEPDNPRLLLEAGRFYAEDNQN
                     KESAAAFEKVLKTDIPAETRPIVENYLSELGKRRRWHGQISLGYGYNSNVNQGNGINQ
                     CVWEIAGMCLMERTLPAPTDSTFSSYSATAEKTVPLKGHHGVQVRGVLYGNRYTEKDK
                     DSAAMPDYGYRNGSLYAGYAYADARSSFSLLPYFEYDFRNRHTHYRAWGADADWSRTL
                     SPHWRINSHAGAKKTGYGGQSKTYFADFKQYELGAGAEFSITPKSGLLVNFDAARKAY
                     PEKSSSSKEYTARLGAYRLFSGGTYLNAVLLYRRSLYDAASFVSDNKRRSDKQYIMMA
                     AAGFPQWNIKGVYPELRFRRTIAHSNAVYYRYRQNEWLLGFKYRF"
     gene            complement(3304..4287)
                     /locus_tag="WNA25_001489"
     CDS             complement(3304..4287)
                     /locus_tag="WNA25_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824220.1"
                     /translation="MSIRNKTLLAVLLCGVFGVAAAAPAGGSSQRKSQDPNSDPTTVR
                     PGTSDKQPFGAGKAGLAFSINNGGSWSKSINLDDGPVKYIRAKYQAPINGNDPYANVD
                     GTRNLQYLRLGDVKPVIGWFYNPTLGQVWYENHHNNTEVYSVRQIANPAIPAAPKFGG
                     LVIAKVPGMGGSNGNVFFGEWAPRAGNPQQISTDLNMADGKRTVWYVGDNPTGNTTGV
                     RTATYNVLGINKHTPGQNDFYTGQVTATFGSGITGTMAGRISRSSDHVDFDHAKIDNR
                     NGTFADPSQGMTGRFYGNQAAAMAGYTTRGTGNVGDDVAFGGSATDIETRK"
     gene            complement(4502..7930)
                     /locus_tag="WNA25_001490"
     CDS             complement(4502..7930)
                     /locus_tag="WNA25_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFA9824221.1"
                     /translation="MMSGFSPKPKTIILSLAGAFGALAFADTPNNTEQQKELNTIVVH
                     GKRSADQKGADDVYYKNVSNAYVGKEYLERYRVQSAGDVLKGLNGVYNMNTRTAGGAI
                     TPNIRGITGKGRIPVTIDGTEQTIDVWMNNYGVGDRNYLDPALFRSIAVEKSPALTRG
                     VKSGVGGAMSIRTIEAGDIVPEGQKFGFQLKTEFANNRTRPANNLNQWLGWEDYRTLP
                     LGATADGAGGGSDPITGQQSPQALVVDNFTPPQHKSGRDNWRFGGDRSYMAAAAFKTE
                     LSDGLAAYSYRNKGNYFAGRKGAEGYLNNPVYDLQKCYDEGGSDFNCKNSATFIPNMA
                     RIYHPGVEVLNSNTETKTLLLKNNWHLPGSHRLGWQYMRTDVRFGEINPFHTTYVMNM
                     EEHNETNRPKELSPQAQSIDSTIRTDTYKLGWAWKPENSRWVDLQANLWRIKTNSTRH
                     QSGGMDLSSARPDPFYDVWYWCTQRGRIPPEHVDNYSSCNDLMNDFGVNGLTKEQVLA
                     MTPNDNGQFRVISGAEQKTRVSRTGFDISNRFRLRDRLSMTLAADYQKEKLAEEVEIV
                     NSQDLFNLAGMVTGLTKLAGPRGGERREWGTNLVFDWQAADRLKISAGIRYHNFRGFD
                     TALAEGRARRDPRYQAGDGTQHYADGAYLPYFELAGDQERRDWNVVAEQLRQAYQSGD
                     AAAIAAAVQAEQAHGARYHLLKYYDGSYRTDGYVYIGNDGRAYFDGRFGGVNEAQNQP
                     LYRVRPVYVPFVNGKLDSGALPQHFHDFVSNYQEKVTNPQGLHGTHYRYWAGVGNPID
                     CIRSDAASCLAHNLSGKVTNFQVGYPEYGDDTGLGLKIIGRHYTEEQYWAQPKPIRAH
                     AWAPTIAVSYDLTDNSRLFVRYAQMSRFPSVYEVGSFYNDVAYAGMPKAPNFRFKPER
                     SRSWEIGYSFNFAPYWSRLRAGDMRLTYYRNRIQNVIETTDYFRTVQYDRKDTAGLEW
                     QSRIDTGRFFAALGATYRLKQQMCDRDTAFDYDPYGAKGVPICIEGGYGSTRGYQALQ
                     PKYSINLDAGVRLLGEKLELGLRGIYHSRVNTKQYDQLLQKDLGIIFDTTGKPHHWRS
                     SLTWDVYGRYRLHKNLNVNLGITNLTNRYYLDPMSNVAAPGPGRTVTFGLTAKF"
     gene            complement(8138..9013)
                     /locus_tag="WNA25_001491"
     CDS             complement(8138..9013)
                     /locus_tag="WNA25_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFA9824222.1"
                     /translation="MAYLLISIVFSVSVSILLKMARKKKIDIAQAVAVNYVVAVILTL
                     SVLKPDVGNIGAFLPTWPLFAALGVLLPSVFVIMGKSVESAGIVKSDAAQRLSLFLPI
                     VAALTLFGEKLSEGKLIGLCLAFAALFCLLWKSDSGKKSGSAWRQALLLLGVWAGYGI
                     IDILFKQIAKSGTAFAGNLLVAFALAGVLMFACLFAKSVRWRVESVVGGILLGGLNFM
                     NIVTYITAHQMMKDSPTLVFAGMNIGVIVLGTLSGALFFKEKINTINTAGIVLSLCSI
                     ACLFYWTEVKALFGI"
     gene            complement(9017..9709)
                     /locus_tag="WNA25_001492"
     CDS             complement(9017..9709)
                     /locus_tag="WNA25_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HFA9824223.1"
                     /translation="MDKDLYAVLGVSPQAGADEIKRAYRKLAMKYHPDRNPGNLQAEE
                     KFKEIQRAYDTLSDLSKRTQYDASFRRHEERGRQEEAFRREQARREQFYGEQMRREQA
                     FRREFERQASRSRHAYEPSGSGSGRNYILAAYILFGLGAIMQFMPIVGVILAYMKRNS
                     LDSIVYAAHTEYLIKTFWRTFWLYLLGALTARFGIGVLVIIATTVWYFYRIIAGFIRF
                     NDGRAVAPEKWI"
     gene            complement(9731..10210)
                     /gene="ybaK"
                     /locus_tag="WNA25_001493"
     CDS             complement(9731..10210)
                     /gene="ybaK"
                     /locus_tag="WNA25_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFA9824224.1"
                     /translation="MSKHTYPITPAVRVLRENGIEFEPFTYAYEEHGGTAQFARLFGK
                     DEHLVIKTIVLQDENGKGPIVLMHGDKQISTRNLARHLGAKHIEPAAPAQANKWTGYL
                     VGGTTPFGIRTKLDIYVEQSVMDLETIYINGGKRGFIIGIRPDDLNILNPKTIQAAV"
     gene            10285..10647
                     /locus_tag="WNA25_001494"
     CDS             10285..10647
                     /locus_tag="WNA25_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFA9824225.1"
                     /translation="MDIRYFGTTPRYSEAVCAGGLIFLSGMVPENGETAAEQTADVLA
                     QTDRWLAECGSDKAHVLDAVIYLRDMGDYAEMNGVWDAWVAAGRTPARACVEARLARP
                     EWRVEIKITAVKRDAATA"
     gene            10681..11544
                     /locus_tag="WNA25_001495"
     CDS             10681..11544
                     /locus_tag="WNA25_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HFA9824226.1"
                     /translation="MKTLLPFFGVARVSGEDRQTFLHGQLSNDINNLQTGQACYATYN
                     TPKGRVIANMIVVNRGDDLLLIMAQDLLEATVKRLRMFVLRAKAVFEILEDYAVGAEL
                     AASAEPLAAQEPSLAFTSECVSDGICSVILHHRGILHIAPETALPPYDAAAENAWRLH
                     EIRSGYPWICAATKETAVAQMLNQHIIGGVHFKKGCYPGQEIIARAQYRGQVKRGLAV
                     LSGNSAAEAGILLTADGEEAGIVLDSVQDSENFTALAVIKFSAAQKELTVPNGGIFKA
                     VHLFFKTKNAE"
     gene            11636..12595
                     /gene="ttcA"
                     /locus_tag="WNA25_001496"
     CDS             11636..12595
                     /gene="ttcA"
                     /locus_tag="WNA25_001496"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFA9824227.1"
                     /translation="MSKKTKQELENNKLSKRLRHAVGDTINDFNMIEPGDKIMVCLSG
                     GKDSYALLDILRRLQASAPIDFELVAVNLDQKQPGFPEEVLPTYLESIGVPYKIVEED
                     TYSTVKRVLDEGKTTCSLCSRLRRGILYRTAKELGCTKIALGHHRDDILATMFLNMFY
                     GGKLKAMPPKLVSDNGEHIVIRPLAYVKEKDLIKYAELKQFPIIPCNLCGSQPNLQRQ
                     VIGDMLRDWDKRFPGRIESMFSALQNVVPSHLADTELFDFAGLERGQNLKHGGDLAFD
                     SEKMPERFSDGSEEDESEIKIEPQKAERKVINILANKPKTCGA"
     gene            complement(12689..15628)
                     /locus_tag="WNA25_001497"
     CDS             complement(12689..15628)
                     /locus_tag="WNA25_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HFA9824228.1"
                     /translation="MSGFNYEKNQPHQMRAVSAVLGVFDGATPKYRTADENPELLFAA
                     KQYANNILKVQSQNGIDGRFPDRSDDQNILDISMETGTGKTYTYTQTMFELHRWLGVF
                     KFIVVVPTLSIKAGTQQFLQSKALAEHFEQDFGGDYEGVRLKTYVVESAKKNKGKKSN
                     APITIEQFVKAENKKEIHVLLINAGMVNSSSMNDTGDKALKDLFDNPVDALAAVRPFM
                     IVDEPHKFPTRDSAKTWGNIKRLKPQYILRYGATFNDEYYNLLYRLTAVDAFNDGLVK
                     GVRVFQEEMQGGMDAAVKLVSSDGKEAKFELNEKDKKQTFKLAKGEDLAQIHPAISDL
                     KIDKMNKTVVVLSNGLELKTGAVINPYSYSQTVQDAMMQRAVAEHFKLERALLAERAP
                     QPKIKPLTLFFIDDIAGYRSGNELSGSLKDKFESWIRAEAARRLKTESDPFYRDYLQK
                     TLDDVSACHGGYFSKDNTDSDDRIEQEINEILHDKEKLLSLDNPRRFIFSKWTLREGW
                     DNPNVFQICKLRSSGSTTSKLQEVGRGLRLPVNELMARVRDVPYKLNYFVDSSEKDFV
                     KQLVGEINDNSFQEEISKKFTEELKQKILQKYPDIKPLVLVNQLFSDGIIDDNENFAE
                     DGYDKLKAAYPEAFPKGLDKGKVSNAKDEGKDTIIMREGKYEELKALWELIHHKAVLQ
                     YKIKDEAEFVDLFTAYLRENAAKFPQAGICTAVNEAYINNGLMLSRRIDSIEDEDFIR
                     FNTMTYREFLEKLAQTAKIQMQTLHQAFYRVRDELNIGDFLNMQTIAQIKNGFNRFLL
                     HHSFHKFELDYRLVGSKIHPTKFTNKDGKPRAVKKADLGRFEDTEHRPAAGYLFGEIF
                     YDSDIEHENVANNQIEGVIVFTKIPRNSIKIPVAGGGTYSPDFAYIVKTKSGEILNFV
                     IEAKGTDGAEDLRKSEERKIKHAEKLFAEISKEIKVVFKTQFDGERIAELIGQNMPAG
                     GHSENGH"
     gene            complement(15718..17514)
                     /locus_tag="WNA25_001498"
     CDS             complement(15718..17514)
                     /locus_tag="WNA25_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFA9824229.1"
                     /translation="MNWLGKSYARLLRNLPPETLISEDKTHNAKPENAGSQNLLIRGD
                     NLEVLKHLKNAYANSVKMIYIDPPYNTGSDGFVYQDDRKFTPAELALLANIDEDEAAR
                     ILDFTDKGSNSHSAWLTFMYPRLYIARELLKDDGVIFISIDDNEAAQLKLLCDEVFGE
                     GNFVAQLPWRKRTAKSDVPFGISQDYEWIFVFAKSCQFIAATKGKERRYYETDDFPDR
                     PWRTHDLTKQTTAAERPNSFFTMVDPKTGKKYPANPNATWRVTKDTFQDYYNKGKIVF
                     PDDYDFLNISNPVMRYFKDDDMKKAGEDFGKVAVSSRLPENVGTLADAVAEYLAIFSR
                     TLPENIGMTKEGTKEITDLFGSKIFTFPKPSQLIKFLVSISSKSNDLILDFFAGSGTT
                     AHAVMQLNAEGQNGNRRYICVQLPEKTAEKSEARKAGYPTIFDITKARIEKAAAKIRV
                     EHPDYTGDSGFKIFQTADNFRQHPDKDFSPEQPDLPLNDELSEEQLQTLLTTWTLYDG
                     AALTTPVEPVRLGSYTAYLCEKRLYLLNKGFTSADLLAFIKKLDDDADFNPNRVIVFG
                     SNMASAMQHELDQAVRGYTNKKEIELNVIIRV"
BASE COUNT         3905 a         4707 c         4630 g         4455 t
ORIGIN      
        1 tatagtgttc gtcctgctgc ggcatctttc cgatggcgca ttttttcaat attaattatc
       61 cgacttacaa aggtaaaaac atgactgtcc gtatcgaaca cgatttattg ggcgaccgcg
      121 agattcccgc cgaagtgtat tggggcatcc acaccctgcg cgccattgaa aactttaaaa
      181 tctccacaca aaaaatttcc gacgtgccgc agtttgtccg cagtatggtg atggtgaaaa
      241 aagcaaccgc gcaggcaaac ggcgaattgg gtgcagtaaa acccgaaatc gccgccgcca
      301 ttgaaaaggc ttgcgacgaa gttctgctga acaaccgctg cctcgaccaa ttcccgtccg
      361 acgtgtatca gggcggggcg ggaacttcgg tcaatatgaa caccaacgaa gtcatcgcca
      421 accttgcatt ggaagccttg ggctatgaga aaggccgcta cgacatcgtc aatccgatgg
      481 atcacgtcaa cgccagccaa tccaccaacg atgcctatcc cacgggcttc cgccttgccg
      541 tgtattacag catcggcgaa ttgctcgaca aactgaccgt attgaaaaac gcctttgccg
      601 ccaaagccga agcgtttaaa gacgttttga aaatgggtcg cacccagctt caagatgccg
      661 taccgatgac ggcaggccag gaattccaat ctttccaagt attgttggaa gaggaaatcc
      721 tcaaccttga ccgcacccgc caactgctct tggaagtcaa tttgggcgca acggcaatcg
      781 gcacgggcgt gaacacgccc aaaggctacg ccgaactggt ggtcaaaaaa ctctccgaag
      841 tcagcggctt gccttgcaaa ctgactgaaa acctgatcga agcgacctcc gactgcggtg
      901 catatgtgat ggtacacggc gcattgaaac gcacggccgt caaactctct aaaatctgca
      961 acgatttgcg ccttctctct tccggtccgc gcgccggttt gaaagaaatc aacctgcccg
     1021 aattgcaggc cggttcttcc atcatgcctg ccaaagtcaa tcccgtgatt cctgaagtcg
     1081 tcaaccaagt ctgcttcaaa gtcatcggca acgatacgac gattaccttc gccgccgaat
     1141 ccgggcaact gcaattaaac gttatggagc cggtcatcgc ccaatgtatg tttgaaacca
     1201 tttccctctt gggcaatgcc gcagtcaacc tatccgacaa atgcgtcaaa ggcattacgg
     1261 tcaaccgcga aatctgcgag cgttacgttt tcaactccat cgggctggtg acttatctga
     1321 atccgtacat cggccaccgc aacggcgact tggtcggcaa aatctgcgcc cagaccggca
     1381 aaggcgtgcg cgaagtcgta ctggaacgcg ggttgttgag cgaagaagaa ctcaaccgca
     1441 tcctctcccc cgagaacctg atgaatcctc atctgtaaac attacggaca aatgccgtct
     1501 gaaaggaagc gccctttcag acggcatttt ttatcacccg actggtcaaa accgatattt
     1561 aaaacccaac agccattcgt tttggcggta gcggtaatac accgcgttgc tgtgtgcgat
     1621 tgttcgcctg aaacgcagtt cgggatatac gcctttgata ttccattgcg gaaaacccgc
     1681 cgcagccatc atgatgtatt gcttgtcgct ccgccgtttg ttatcgctga cgaaacttgc
     1741 cgcgtcatac aggctgcggc ggtagagcag gacggcgttt aaataagtac cgcccgaaaa
     1801 aagcctatac gcgcccagcc gagccgtata ttctttgctg gaagaggatt tttcgggata
     1861 ggctttgcgt gcggcatcga aatttacaag caggccgctt ttcggcgtga tggaaaattc
     1921 cgcccccgcg ccgagttcgt attgtttgaa atcggcaaaa taagttttgc tttgtccgcc
     1981 gtatccggtc ttttttgctc cggcatgtga gttaatgcgc cagtgcgggg agagtgtgcg
     2041 cgaccagtcg gcatccgcac cccatgcgcg gtagtgggtg tggcggttgc ggaagtcgta
     2101 ttcgaaataa ggcagcaggg aaaaggagga gcgtgcgtcg gcataggcgt atcccgcgta
     2161 cagcgaaccg ttgcgatagc cgtaatccgg catggccgcc gaatctttgt ctttttccgt
     2221 gtagcggttg ccgtacagta caccgcgcac ctgtacgccg tgatgccctt tgagcgggac
     2281 ggttttttcg gcggtcgcgc tataggatga gaaggtcgaa tccgttggcg cgggcagggt
     2341 gcgctccatc aggcacatgc ccgcaatttc ccacacgcat tggttgatgc cgttgccttg
     2401 gtttacattg ctgttatagc cgtagccgag gctgatttgg ccgtgccagc ggcggcgttt
     2461 gcccagttcg gacaggtagt tttccacgat ggggcgggtt tctgcgggaa tgtccgtttt
     2521 cagaactttc tcaaacgcgg cggcagactc tttgttttgg ttgtcttcgg cataaaaccg
     2581 ccccgcttcg agcagcaggc gcgggttgtc gggttcggct tccaaggccg tctgaaaact
     2641 gttccccgcc gcgcggaaat ccccttcgtc ccgtttttgc agcgcatcgg ccaaatgaat
     2701 cagcgcgggc ttgtgccggg gcagtttggc gtagcgggcg gcgaactgcc gcaccttgtg
     2761 ccactgccgc gcattgaggg cgtggtaaat tgcgctttcc agttcttccg ccgtatcgcc
     2821 gacttggtag atttcgccgc ctatcgaaat atagccgtcg ttttcctcta cttccccgcg
     2881 tgcctgttcc gtgtccagcc aaccgctttc gcggtattgc tgcgtttggc ggctgccctc
     2941 gtccagcagg cggcgttcgt cggcggggtt gtcggcgtgg gcggtgatgg gcagaagcag
     3001 gcagaagagg gcgaggctga gtggtttgtt catggttgcc ttccgaaaag ataaagtaac
     3061 gcaggtttta attgctgtcc caaaatgtcc gataggccga aaccgtatgg gataggcgat
     3121 gtttatttac aaatatcaaa tttcaggtag cctgtgttga acacgggcta cctgaaaccg
     3181 agcagcaatc cgtatcggac ggatgattta tatggcttgg cagtcggcat aaaacggcag
     3241 cttgaaactc ctcaggggtt aaatcgagcc tagccgttgc caaaccaaat cggatggcag
     3301 ctgttatttg cgggtctcaa tatcggtggc gctaccgccg aaagctacgt catcgccgac
     3361 attaccggta ccacgtgtgg tatagccggc catggctgcc gcctggttgc cgtagaagcg
     3421 gccggtcatg ccttgactgg gatcggcaaa ggtaccattt ctgttatcga ttttggcgtg
     3481 atcaaaatcg acgtgatcgg aactgcggct gattctgcct gccatcgtcc cggttatacc
     3541 agaaccgaag gtggcggtta cctgaccggt atagaaatcg ttctgccccg gggtgtgctt
     3601 attgataccc agcacattgt aggtggcggt tctcacaccg gtggtgttgc cggtgggatt
     3661 atcgcccaca taccaaacgg tgcgtttacc atccgccata ttcaggtcgg tgctgatttg
     3721 ctgcgggtta ccggcacgcg gcgcccattc gccgaagaag acgttgccgt tgctgccgcc
     3781 catgcccggc actttggcga ttaccaggcc gccgaacttg ggtgcggcgg ggattgccgg
     3841 gttggctatc tggcgcacac tgtacacttc agtgttgttg tggtggtttt cataccacac
     3901 ctgacccaac gttgggttgt agaaccagcc gataaccggt ttcacatcgc ctaagcgcag
     3961 atattgcaga ttgcgcgtac catcaacatt agcataggga tcgttgccgt taatcggtgc
     4021 ctgatatttc gcacggatat atttgaccgg accatcatcc agattgatgg atttcgacca
     4081 acttccaccg ttattgatac tgaatgccaa accggcctta ccggcaccga aaggttgctt
     4141 gtcagaggtg ccggggcgaa ctgtcgtcgg gtcactattt ggatcttggc ttttcctttg
     4201 gctggaacca ccagccggag cagccgcagc cacaccaaaa acgccgcaca gcaagacggc
     4261 caacagggtt ttattacgta tagacataat cgtttcctta attaagagtt aaacaaaagc
     4321 caccgtttta ttaccgccga accggcggaa aaaacggtaa aaaccattac ctcggataag
     4381 cggggccggt tgggtttgtg cgtccggagt gttcaattgt ttgttgttat atccaatccg
     4441 ttttcaggta gccttcccgc catacccgca ggctacctga aaaccccact ccgccctgcg
     4501 gctagaactt ggcagtcagc ccgaaggtaa ccgtccggcc cggcccgggg gcggcaacgt
     4561 tggacatcgg gtcgagatag tagcggttgg tcaggttggt gatgccgagg ttgacgttga
     4621 ggtttttgtg cagccggtag cggccataaa catcccaagt cagcgatgac cgccaatggt
     4681 ggggcttgcc ggtggtatcg aagatgatgc ccaaatcctt ttgcagcagt tggtcgtatt
     4741 gcttggtgtt gacccggctg tggtagatgc cgcgcaggcc gagctccagt ttctcgccca
     4801 acaggcgcac cccggcatcc aggtttattg aatatttcgg ctgcaaggcc tgatagccgc
     4861 gcgtactgcc gtagccgcct tcgatgcaga tcggcacgcc tttggcacca taagggtcgt
     4921 aatcgaaagc ggtatcgcgg tcacacatct gctgtttcag gcggtaggtg gcaccgagcg
     4981 cggcaaagaa gcggccggtg tcgatgcggc tctgccattc cagcccggcg gtgtccttgc
     5041 ggtcgtattg cacggtgcgg aaataatctg tggtttcgat cacattttgg atacggttgc
     5101 ggtagtaggt caggcgcata tcgccggcac gcagccggct ccaatacggg gcaaaattga
     5161 agctatagcc gatttcccag ctgcggctgc gttccggctt gaagcggaaa tttggggcct
     5221 tcggcatacc ggcataggcc acgtcgttat aaaaactgcc cacctcatac acgctgggga
     5281 agcggctcat ctgcgcatag cgtacgaaca ggcggctgtt gtcggtcaga tcgtagctga
     5341 cggcgatggt cggcgcccag gcatgggcac ggatcggttt gggctgcgcc caatactgtt
     5401 cctcggtata gtgccgaccg atgattttga gaccgagacc ggtatcatca ccgtactcgg
     5461 gatagccgac ttgaaaattg gttacttttc ccgataggtt atgagcaagg caagaggccg
     5521 catcacttct tatacagtcg ataggattcc ccacgcctgc ccaatatcga taatgcgtcc
     5581 cgtgcagccc ctgcggattg gtcacttttt cctgataatt gctgacgaaa tcatgaaaat
     5641 gctggggcaa tgcgccgctg tccagcttgc cgttgacaaa cggcacataa acggggcgca
     5701 cccgatagag cggttggttc tgtgcctcgt taaccccgcc gaaccgtccg tcaaaatagg
     5761 cacggccatc gttgccgata tacacataac catcggtacg atacgagccg tcatagtatt
     5821 tcagcaaatg gtaacgggcg ccgtgcgcct gttctgcctg tactgccgcg gcaatggcgg
     5881 cagcatcgcc tgactgatac gcctgccgaa gctgttcggc caccacattc cagtcacgcc
     5941 gctcctgatc gcctgccagt tcgaaatacg gcagataggc accatcggca taatgctggg
     6001 ttccatcgcc tgcctgatag cgcgggtcgc ggcgggcacg cccttcggcc agggcggtat
     6061 cgaagccccg gaagttgtgg tagcggatgc cggcggagat tttcaggcgg tcggcggcct
     6121 gccagtcgaa caccaaattg gtgccccatt cgcgccgctc gccgccgcgc ggcccggcca
     6181 gcttggtcag gccggttacc atgccggcca ggttgaacag gtcttgcgaa ttgacgattt
     6241 ccacttcctc cgccagtttc tctttctgat aatcggcggc caaggtcatg ctcaggcggt
     6301 ctctgaggcg gaagcggttg ctgatatcga aaccggtgcg gctgacgcgg gttttctgct
     6361 cggcgccgga aatcacgcgg aattggccgt tgtcgttcgg ggtcatggcc aacacctgtt
     6421 ctttggtcag gccgttgacg ccgaaatcat tcatcaggtc gttgcagctg ctgtagttgt
     6481 cgacgtgctc gggcggtatc cggccgcgtt gggtacacca ataccaaacg tcgtagaagg
     6541 gatcggggcg ggcggaagac aaatccatgc cgccactctg gtggcgggtg ctgttggttt
     6601 tgatgcgcca caggttggcc tgcagatcga cccagcggct gttttccggc ttccaggccc
     6661 agccgagttt gtaggtgtcg gtgcggatgg tggaatcgat gctctgcgcc tgtggcgaca
     6721 gttctttggg gcggttggtc tcgttgtgct cttccatatt catcacatag gtggtgtgga
     6781 aggggttgat ctctccgaag cgcacgtcgg tgcgcatata ttgccagccg agccgatggc
     6841 tgccgggcag gtgccagttg tttttcagca gcagggtttt ggtttcggtg ttgctgttga
     6901 gcacttccac cccggggtgg tagatgcgcg ccatattggg gatgaaggtg gcggaatttt
     6961 tgcagttgaa atcgctgccg ccttcgtcgt agcatttttg caggtcgtat accggattat
     7021 tcaggtagcc ttccgcaccc ttgcgaccgg cgaagtagtt gcctttgttg cggtagctgt
     7081 aggcggcaag gccgtctgaa agttcggttt tgaaggcggc ggcggccata taactgcggt
     7141 cgccgccgaa gcgccagttg tcgcggccgc ttttgtgctg cggcggggta aagttgtcta
     7201 ccaccaacgc ctgcgggctc tgctgtccgg tgatcgggtc agaaccgccg cccgcgccgt
     7261 cggcggtggc gccgagcggc agggtgcggt agtcttccca gcccagccat tggttgaggt
     7321 tgttggccgg gcgggttctg ttgttggcaa actcggtttt cagctggaag ccgaatttct
     7381 gcccttcggg cacgatgtcg ccggcttcga tggtgcggat ggacatcgcg ccgccgacgc
     7441 ccgatttcac gccgcgcgtg agggcggggc ttttttccac cgcgatgctg cggaacaggg
     7501 cggggtcgag gtagttgcgg tcgcccacgc cgtagttgtt catccatacg tcgatggttt
     7561 gttcggtgcc gtctatggta accgggatgc ggcccttgcc ggtgataccg cgtatgttgg
     7621 gcgtgatggc gccgccggcg gtgcgggtgt tcatgttgta cacgccgttt aggcctttga
     7681 gtacgtcgcc ggcggattgg acgcggtagc gttcgaggta ttctttgccg acgtaggcgt
     7741 tggagacgtt tttgtaatac acgtcgtccg cgcctttttg gtcggcactg cgtttgccat
     7801 ggacgactat ggtattcagt tctttctgct gttcggtatt gttcggggtg tctgcaaaag
     7861 ccaatgcgcc aaatgcacct gcaaggctta ggataatggt cttaggtttg ggggagaatc
     7921 cggacatcat gtattcctta ttaagaatat aaaatgaatt ttattaagat acactcataa
     7981 taagaattat ttgaaatttt taattattat taatatttaa attataatta aaaataacta
     8041 ttattaatta aattttgaat ctgcccgtat aacaaaatgc cgtctgaagc agcattttcc
     8101 tgtttcagac ggcattttgt tatatcaatc agccggatta tatgccgaac agtgccttaa
     8161 cttctgtcca atagaacagg caggcaatgg agcataatga cagtacaatg cctgccgtat
     8221 tgattgtgtt gattttttcc ttaaagaaca atgcgcccga aagcgtccct aaaacaatca
     8281 caccgatatt cataccggca aaaaccagcg tgggactgtc cttcatcatt tggtgcgcag
     8341 tgatgtaggt tacgatgttc ataaaattca aaccgcccaa gagtatgccg ccgaccacgc
     8401 tctcaacgcg ccatctgacc gatttggcaa acaggcaggc aaacatcagc acacccgcca
     8461 gcgcaaatgc aaccagcagg ttgcccgcaa atgccgtgcc gcttttggca atctgtttga
     8521 acaggatgtc gataatgccg taacccgccc acacaccgag cagcaataac gcctgccgcc
     8581 acgcgctgcc tgattttttg ccgctgtcgc ttttccaaag caggcagaac agtgcggcaa
     8641 aggcgaggca cagtccgatc agtttgcctt cactgagttt ttcgccaaac agcgtcaaag
     8701 cggcaacaat cggtaaaaac agcgacaaac gctgcgccgc gtcggatttg acgataccgg
     8761 cggattctac ggatttgccc ataatgacaa atacggacgg cagcagcacg cccaaagcgg
     8821 caaacagcgg ccacgtcggc aaaaatgcac cgatattgcc gacatccggc ttcaataccg
     8881 acagggtcag tatgaccgcg accacataat tgacggcgac cgcctgcgcg atgtcgattt
     8941 ttttcttcct tgccattttc agcaaaatgg aaaccgacac gctgaacacg atgctgatta
     9001 acaggtaggc catgctctat atccatttct cgggcgcaac cgccctgccg tcgttgaagc
     9061 ggataaagcc ggcgatgatg cggtagaaat accagacggt cgttgcaata ataaccagca
     9121 cgccgatgcc gaaacgggca gtcagcgcgc ccaaaagata aagccaaaat gtgcgccaaa
     9181 aggttttaat caggtattcg gtatgtgcgg catagacaat gctgtccaaa ctgttccgtt
     9241 tcatataggc gagaatcacg ccgactatgg gcatgaactg catgattgca cccaaaccga
     9301 acaggatgta ggcggcgagg atatagttgc gcccgcttcc gctgccggac ggttcgtaag
     9361 catggcgcga acgtgaagcc tgccgttcaa actcccgtct gaacgcctgt tcgcggcgca
     9421 tctgttcgcc gtaaaactgc tccctgcgcg cctgttcgcg gcggaatgcc tcttcctgcc
     9481 gcccgcgttc ctcatgccgt ctgaaggacg cgtcgtattg cgtccgtttt gacaggtcgg
     9541 aaagcgtatc gtaagcccgt tggatttctt tgaacttttc ttccgcctgc agattgcccg
     9601 gattgcggtc gggatggtat ttcatcgcca gcttgcggta ggcgcgtttg atttcgtccg
     9661 cccccgcctg cggcgacacg cccaatacgg catacaaatc tttgtccata ttgctcctta
     9721 tacttcccgg tcaaaccgct gcctgtattg ttttcgggtt caaaatattt aaatcatcgg
     9781 ggcggatacc gataatgaac ccgcgttttc cgccattgat atagatggtt tccaaatcca
     9841 tcaccgactg ttcgacgtaa atatccaatt ttgtccggat gccgaacggc gttgtgccgc
     9901 cgaccagata gcccgtccac ttgtttgcct gcgcaggcgc ggcgggttcg atgtgtttcg
     9961 cacccaaatg ccgcgccaga ttgcgggttg aaatctgttt gtcgccgtgc atcaagacaa
    10021 tcggcccctt accgttttca tcttgcaaaa caatggtttt aatgaccaag tgttcgtctt
    10081 tgccgaacag tcgggcaaac tgcgccgtgc cgccgtgttc ctcataggca taggtaaaag
    10141 gttcaaattc gatgccgttt tcacgcaaaa cgcgtacggc aggtgttatc ggataagtgt
    10201 gtttactcat ttttcgtcat gcaaaacatg gaaatcggtc aagcgcaatg ttaccatata
    10261 tcccctttat cgaaggaaac cgatatggat atccggtatt tcggcacaac gccccgctat
    10321 tccgaagcgg tttgcgcggg cggcttgatt ttcctctccg gcatggttcc cgaaaacggt
    10381 gaaacggctg ccgaacagac tgccgacgtg cttgcccaaa ccgaccgctg gctggcggaa
    10441 tgcggctcgg acaaggcaca cgttttggat gcggtcatct acctgcgtga tatgggcgac
    10501 tatgcggaaa tgaacggcgt gtgggacgct tgggttgccg ccggcagaac gcccgcccgc
    10561 gcctgcgtgg aagcgcggct cgcccgtccg gagtggcgcg tcgaaatcaa aataaccgcc
    10621 gtgaagagag acgcggcaac tgcataaaca gatttcctta tatttcagag tacgcaaatg
    10681 atgaaaacct tactgccctt tttcggcgtg gcgcgcgtca gcggcgaaga ccgccaaacc
    10741 ttcctgcacg gacagctttc caacgacatc aataatcttc agaccgggca ggcgtgttac
    10801 gcgacttaca acacgcccaa aggccgcgtc atcgccaata tgattgtcgt caaccgtgga
    10861 gacgatttgc tgctgattat ggcgcaagat ttgcttgaag caacggtcaa acgcctgcgg
    10921 atgtttgtgt tgcgcgccaa agccgttttt gaaatccttg aagattacgc cgtcggcgcg
    10981 gaattggcag catccgccga accgcttgcc gcgcaagaac ccagtctcgc ctttacctcc
    11041 gaatgcgtct cagacggcat ctgttccgtc atcctgcacc atcggggcat tttgcacatc
    11101 gcccccgaaa ccgccctacc cccttatgac gctgccgccg aaaacgcgtg gcggctgcac
    11161 gaaatccgca gcggttatcc gtggatatgc gcggctacca aagaaaccgc cgtcgcccaa
    11221 atgctcaacc agcacatcat cggcggcgta cacttcaaaa aaggctgcta cccgggacag
    11281 gaaatcatcg cgcgcgccca gtatcggggg caggtcaaac gcggcttggc ggtattgtcg
    11341 ggaaattcgg cagccgaagc gggcatcctg ctgacggcgg acggcgaaga agccggcatc
    11401 gtactcgaca gcgttcaaga ttcggaaaac tttaccgccc ttgccgtgat taaattctcc
    11461 gccgcgcaaa aagaactcac cgtcccaaac ggcggtatct tcaaagcggt tcatctcttc
    11521 tttaaaacca aaaatgcaga ataagccctc taatgccgtc tgaagccctt cagacggcat
    11581 tgccgcccct gttttcggtt acaatgccgc atccctaatt tttcaaacca ccgccatgtc
    11641 caaaaaaacc aagcaagaac tcgaaaacaa caaactcagc aaacgcctgc gccacgccgt
    11701 cggcgacacg attaacgatt tcaacatgat cgagccgggc gacaaaatca tggtctgcct
    11761 ctccggcggc aaagacagct acgccctgtt agacatcctg cgccggctcc aagccagcgc
    11821 gccgattgat tttgaactgg tcgccgtcaa tctcgaccaa aaacagccgg gcttccccga
    11881 agaagtattg ccgacctatc tcgaaagcat cggcgttccc tacaaaatcg ttgaagaaga
    11941 cacttactcc accgtcaaac gcgtgttgga cgaaggcaaa acgacttgtt cgctgtgcag
    12001 ccgcctgcgg cgcggcatcc tctaccgcac cgcaaaagaa ttgggctgca ccaaaatcgc
    12061 cttgggacac caccgcgacg acatcctcgc caccatgttt ttaaatatgt tttacggcgg
    12121 caaactcaaa gccatgccgc ccaagctggt aagcgacaac ggcgaacaca tcgtcatccg
    12181 cccgctggcg tatgtgaaag aaaaagactt aatcaaatac gccgaattga aacagttccc
    12241 catcatcccg tgcaacctct gcggctcgca gcccaaccta caacgccaag tcatcggcga
    12301 tatgctgcgc gattgggaca aacgcttccc cggccgtatc gaatcgatgt tctccgccct
    12361 gcaaaacgtc gtcccttccc accttgccga taccgaactc tttgactttg ccggattgga
    12421 acgcggtcaa aatctgaaac acggcggcga tttggctttt gacagcgaaa aaatgcccga
    12481 acgcttttca gacggcagcg aagaagatga gagcgaaatc aaaatcgagc cgcaaaaagc
    12541 cgaacgcaaa gtcatcaaca ttctggcgaa caagccgaaa acctgcggcg cgtaaacctc
    12601 caccgtcaaa aatgccgtct gaagcccgtt tggcgtttca gacggcattt taaaatcccg
    12661 aagcagacgg cttccaaaca tttgtttctc agtgtccgtt ttcagaatgc ccgcctgctg
    12721 gcatattttg cccgatcagt tcggctatcc tctcgccgtc aaactgcgtt ttgaacacca
    12781 ccttgatttc tttggaaatc tcggcaaaca gcttttcggc atgtttgatt ttccgctctt
    12841 cgctttttcg caaatcttcc gccccgtcag tccctttggc ttcaatcaca aagttcagaa
    12901 tctcgccgct tttggttttc acgatatagg caaagtcggg cgaatacgtg ccgccgccgg
    12961 caacagggat tttgatggag tttctcggta ttttggtaaa tacgattacg ccttcaattt
    13021 ggttgttggc gacattttca tgttctatat ccgaatcgta gaaaatctcg ccgaagagat
    13081 agccggcggc aggccggtgc tccgtatctt caaatctgcc caaatctgct tttttcaccg
    13141 cgcgcggttt gccgtcttta ttggtaaatt tggtcggatg gattttgctg ccgacaagcc
    13201 ggtaatccag ttcgaattta tggaaggaat gatgaagcaa aaaccggttg aagccgtttt
    13261 tgatttgggc gatggtctgc atattcaaaa aatcgccaat gttcagttcg tcgcggacgc
    13321 ggtaaaacgc ctgatgcaaa gtctgcatct ggatttttgc cgtttgtgcc agtttttcca
    13381 gaaactcacg gtaagtcatt gtgttgaaac ggataaaatc ttcatcttca atactgtcta
    13441 tgcggcggga aagcataagc ccgttgttga tataagcttc gtttaccgcc gtgcatatgc
    13501 ctgcctgcgg gaatttggcg gcgttttcac gcaaataggc ggtaaataaa tcgacaaatt
    13561 cggcttcatc tttgattttg tactgcaaaa cggctttatg gtgaatcagc tcccacaagg
    13621 ctttgagttc ttcatatttg ccttcgcgca tgatgatggt gtctttgccc tcgtctttgg
    13681 cgttgctgac tttgcctttg tccaaacctt tggggaaggc ttcgggatag gcggctttta
    13741 atttgtcata gccgtcttcg gcaaagtttt cattgtcgtc aatgatgccg tctgaaaaca
    13801 gttggtttac caataccagc ggtttgatat cggggtattt ttgcaatatt ttttgtttca
    13861 gctcttcggt aaactttttg gagatttctt cctgaaaaga attgtcgttg atttcgccga
    13921 caagctgctt cacaaagtct ttttcgctgc tatcgacaaa ataattcagt ttgtacggta
    13981 catcgcgcac ccgcgccatc agctcgttta ccggcaggcg caggccgcgt ccgacttctt
    14041 gcagcttgga agtcgtgctg ccgctggaac gcagtttgca aatctggaaa acgttgggat
    14101 tgtcccagcc ttcgcgcagc gtccatttgg aaaaaataaa gcggcgcggg ttgtccaaag
    14161 acagcagttt ttccttatcg tgcaggattt cattgatttc ctgctcgatt ctatcgtcgc
    14221 tgtctgtatt gtctttggaa aaatagccac cgtggcaggc ggatacatcg tccaacgtct
    14281 tttgcaggta atcgcggtaa aacgggtcgc tttccgtttt cagacggcgt gccgcttccg
    14341 cgcgaatcca gctttcaaat ttatctttca ggctgcctga aagctcgttg ccgctgcggt
    14401 agcccgcgat atcgtcaata aaaaacagcg tcagcggctt gattttgggc tgtggcgcgc
    14461 gttctgccaa aagcgcgcgt tccagcttga aatgttcggc aaccgcccgc tgcatcatcg
    14521 catcctgcac cgtttgcgaa taggaataag ggttgatgac ggcacccgtt ttcaactcca
    14581 agccgttgct taacaccacc acggttttat tcattttgtc gattttcaaa tccgaaatag
    14641 ccggatggat ttgcgccaaa tcttcgcctt ttgccagttt gaacgtctgc tttttgtcct
    14701 tttcgtttaa ttcaaatttc gcttctttgc cgtccgacga caccagtttt accgccgcat
    14761 ccatgccgcc ctgcatttct tcctgaaaca cgcgcacgcc tttgaccagc ccgtcgttaa
    14821 acgcgtctac tgccgtcaaa cggtaaagca agttgtaata ttcatcgtta aatgttgcac
    14881 cgtagcgcaa aatatattgc ggttttaagc gtttgatatt gccccacgtt ttcgcgctat
    14941 ctcgggtcgg gaatttatgc ggttcgtcca caatcataaa cgggcgcacg gcagccaatg
    15001 catcaacggg attgtcaaac aaatccttca atgccttgtc gcccgtatcg ttcatggacg
    15061 acgaattaac catgcccgcg ttaatcagca gcacatgaat ttcctttttg ttttccgctt
    15121 tgacaaattg ctcaatcgtt atgggcgcat tggacttttt gcccttattc tttttcgcgc
    15181 tttccaccac ataggttttc aggcgtacgc cttcataatc gccgccgaaa tcctgttcaa
    15241 aatgctctgc caaagccttg ctttgcaaaa actgctgtgt tcccgcctta atggacaaag
    15301 tcggcacgac cacgataaat ttgaacacgc ccagccaacg gtgcagctcg aacatggttt
    15361 gtgtgtaggt ataggttttg cccgtgcccg tttccatgga aatatcaagg atattttggt
    15421 cgtccgaacg gtcggggaat cggccgtcta taccgttttg gctttgcact ttcaggatat
    15481 tgtttgcgta ttgttttgca gcaaacaaaa gttcgggatt ttcgtctgcc gtccgatatt
    15541 tgggcgttgc cccgtcaaac acgcccaaaa ccgccgaaac cgcccgcatt tggtgcggct
    15601 ggtttttctc gtaattaaaa ccgctcatga atgtttctcc atgaagtcat tgttgtatcg
    15661 tccttccccc gcaaggggaa tccaagccga aaagcagcct gcactttcgg attccggtta
    15721 caccctgata atcacattca actcaatctc ttttttattg gtataaccgc gaaccgcctg
    15781 gtcgagttcg tgctgcatgg cgcttgccat attgctgccg aatacaatca cacggttggg
    15841 attgaaatcc gcatcgtcgt ccagcttctt gataaacgcc agcaaatcgg cggaagtaaa
    15901 acccttgtta agcagataca gccgtttttc gcacagatac gccgtataac tgcccaaccg
    15961 cacaggctca accggcgtgg tcagtgccgc cccgtcatac agcgtccagg tggtcagaag
    16021 cgtttgcagc tgttcttcgc ttaattcatc gttaagcggc aaatccggtt gttcgggcga
    16081 aaaatccttg tctggatgct gcctgaaatt gtctgccgtt tgaaagattt tgaagcccga
    16141 atcgcccgtg taatcgggat gttcgacgcg gattttggcg gcggcttttt ctatgcgggc
    16201 tttggtgatg tcgaagatgg tcgggtagcc tgctttacgg gcttcggatt tttcagcggt
    16261 tttttcggga agctgtacac agatatagcg gcggttaccg ttttgtcctt cggcgttaag
    16321 ctgcatcacg gcgtgggcgg ttgtgccgct gcctgcgaag aagtctagga ttaggtcatt
    16381 actctttgaa cttattgaaa ctaaaaattt aatcaattga ctaggcttgg ggaaggtaaa
    16441 tattttgcta ccaaataaat ctgtgatttc ttttgtgcct tctttagtca ttccgatatt
    16501 ttcaggtagc gtcctactaa aaatagccaa atattcggct actgcatcgg ctaatgtacc
    16561 aacattttca ggtaatctac tgcttacagc tactttgcca aaatcctcgc cagctttttt
    16621 catgtcgtca tctttaaagt aacgcataac tggattgctg atatttaaga aatcataatc
    16681 atcagggaaa acgatttttc ctttattata ataatcttga aatgtatctt tggttacacg
    16741 ccaagttgca tttggatttg ctggatattt ttttcctgtc ttgggatcaa ccattgtgaa
    16801 aaaactattt ggcctttccg ccgcagttgt ttgtttcgtt aagtcgtggg tacgccaagg
    16861 acgatcgggg aaatcatcag tctcataata gcgtcgttcc ttgcctttag ttgctgcaat
    16921 aaattggcaa gattttgcga atacaaatat ccattcataa tcctgcgaaa taccaaaagg
    16981 cacatctgat ttagctgttc tttttcgcca aggcaattgt gcaacaaaat tcccttcccc
    17041 aaacacttca tcacacaaca atttcaactg cgccgcttcg ttatcgtcaa tcgagataaa
    17101 aatcacgccg tcgtccttta acagttcgcg ggcgatatac aggcgcggat acataaaggt
    17161 cagccacgcg ctgtgcgagt ttgagccttt gtcggtgaaa tctaaaatcc gcgcggcttc
    17221 gtcttcgtca atattcgcca ggagggcaag ttcggcgggt gtgaatttgc ggtcgtcctg
    17281 atagacaaag ccgtccgatc cggtgttgta gggcgggtcg atgtaaatca tcttcacgct
    17341 gtttgcgtag gcgtttttta agtgtttcaa cacttccaga ttatcgccgc gaatcagcag
    17401 attttggctg cctgcatttt cgggcttggc gttgtgcgtt ttgtcttcac ttatcagggt
    17461 ttcgggcggc aggttgcgaa gcaggcgggc atatgatttg cccagccagt tcatttcgta
    17521 aaattcgcgt ccgatgtcgg tctgtggcgc aatttcggct tgtaatctgt cgataaggaa
    17581 atttccgtct gcgtcaaaac aggcgggaaa cagttttttg agctgttcga gttgggtaga
    17641 gttggcggta atgccgtctg aagtgtagat tgcctcagtg ttcgccccgg ctgtgtc
//
LOCUS       DAWTQS010000048        17604 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.048, whole genome shotgun sequence.
ACCESSION   DAWTQS010000048 DAWTQS010000000
VERSION     DAWTQS010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17604
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.048"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            36..248
                     /locus_tag="WNA25_001499"
     CDS             36..248
                     /locus_tag="WNA25_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFA9824230.1"
                     /translation="MELGYTPYNLRTLRNRCKLTQAELAQIVGVKHYIQVGRWEAEPD
                     TETRRADMPLEKWRQFLDWIEKTNAV"
     gene            433..947
                     /locus_tag="WNA25_001500"
                     /pseudo
     CDS             433..947
                     /locus_tag="WNA25_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238080.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
     gene            944..1270
                     /locus_tag="WNA25_001501"
     CDS             944..1270
                     /locus_tag="WNA25_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFA9824231.1"
                     /translation="MTDAENGRGQDTLAHIAQSIRNILFTRIGTRLMREEYGSFIPDL
                     IDMPAGHAAIALIHQAAVTALARWKPRITVRRIQADTADLAAGKIKLTLDVTLADGGE
                     RTYRIK"
     gene            1291..2346
                     /locus_tag="WNA25_001502"
     CDS             1291..2346
                     /locus_tag="WNA25_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFA9824232.1"
                     /translation="MGNSRLSQLPAPAAIEETDFEGIFARKKAALTALCPESIRETVA
                     QTLELESEPLTIDLQQQAYQELLVRNRINEAVKANLLAYAQGSDLDHIAAQYGLSRKT
                     IRAADPDANPPVAAEYETDDAFRARVQAHPEKYAAGPRTAYEAHAIDAHPQITHARAV
                     RRAAGTVEVYIKTQSGTPDETILTAAREYLSAETRRPLCDNVQVTAAQPKDAAVEYSA
                     EYHPAANIQAERQTACEALDNLWRQNAHIGASVALSKIIGALDTPGVKKNHTAQPRRR
                     HRMRQRRIHPNHVHARARINEQHRPREQQPPATRTGKADRTRNCRRLPPTRPRIFTLS
                     RLRKKHRHGRGLGLCRN"
     gene            2765..3270
                     /locus_tag="WNA25_001503"
                     /pseudo
     CDS             2765..3270
                     /locus_tag="WNA25_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358063.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            3702..4952
                     /locus_tag="WNA25_001504"
     CDS             3702..4952
                     /locus_tag="WNA25_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFA9824233.1"
                     /translation="MEKTQFGRHPRRRDRVLSQSRPKPRRLSQSRRHDLCTKHLLDLY
                     RALGNTNKLPDLTRTDIGITAWFPSDQIQTGWLAFDDIRERVTESAYPELYRLLTEKY
                     GRIQNVPQAEDRFILNAGNGLAVGTKQEDEIKRHVHKVFSHWANHPDAAAVGYEDRNE
                     RQRSALVSTWTDGDLNDNGFLTPRLDSKMATGGAENRPKALVLKLCIKAADTLGEAVF
                     RIKSHGETANAGALDASRLAQGLQEKADRDHTHTTAQIQGLDEKISAAVAAQFTRQTI
                     GGVDIVRFPDGTMIQTGSYRFARGGSPIGNEVVFPIAFADGNVKCFVSERHSGRVNGE
                     RQHNWLFIRAKNHAAAIITNWYESSCDWMAIGKSASGNAASPTPIVPEIPEIDEEPQR
                     ESGRSSTGLRNPRRHRGLDFPVGS"
     gene            5064..5393
                     /locus_tag="WNA25_001505"
     CDS             5064..5393
                     /locus_tag="WNA25_001505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824234.1"
                     /translation="MPRQSCHSSPFPRKRESGTQTRQESIGKNNPTAVIPAQAGIQTH
                     NVKAVYQKKPKPNALDFRLRGNDEELGSDERREQPRKKPPTPSDGIGNKNRTAETARE
                     RIAGRAR"
     gene            complement(5409..5720)
                     /locus_tag="WNA25_001506"
     CDS             complement(5409..5720)
                     /locus_tag="WNA25_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HFA9824235.1"
                     /translation="MKMHNPAHPGLVLKEYINGASITDIAKRLGVSRVALSRIVNAQS
                     SITPEMAVRLSQLLNTSPDFWLNMQAGYDLWQIRQRKTFDIAPLFPEPPLSSMKTGAR
                     T"
     gene            complement(5728..6010)
                     /locus_tag="WNA25_001507"
                     /pseudo
     CDS             complement(5728..6010)
                     /locus_tag="WNA25_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221019.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            6092..6705
                     /locus_tag="WNA25_001508"
                     /pseudo
     CDS             6092..6705
                     /locus_tag="WNA25_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237919.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6856..7113)
                     /locus_tag="WNA25_001509"
     CDS             complement(6856..7113)
                     /locus_tag="WNA25_001509"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824236.1"
                     /translation="MPSECGAAAILPRIPFIGGAALVEPRVVLPAFQLADIRLPAVVA
                     VEKYAVDQGIYSGLTKTGTALPRLAVLFVLSAASSPCPDFC"
     gene            complement(7167..7550)
                     /locus_tag="WNA25_001510"
     CDS             complement(7167..7550)
                     /locus_tag="WNA25_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFA9824237.1"
                     /translation="MIYYPAVLFQDPDQSNWGVIIPDLPGCYPVGDTVADALADAKAA
                     AMFHLEGLLQENLPIPEAQSIEVNRDNPDYADAVLWTMVEADDAALTGQVRFNVSWPQ
                     HILNRVDACTAARHETRAVFWRKPP"
     gene            complement(7655..7795)
                     /locus_tag="WNA25_001511"
     CDS             complement(7655..7795)
                     /locus_tag="WNA25_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824238.1"
                     /translation="MPQYRNWQSGLDAKCADGDEYGVAQSDCRTREINAKTKEIQGYL
                     ID"
     gene            complement(7840..8292)
                     /locus_tag="WNA25_001512"
     CDS             complement(7840..8292)
                     /locus_tag="WNA25_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HFA9824239.1"
                     /translation="MALIKEPLDKAKQRNEKLEAAEATAQEAREAEEAAAQEALGREQ
                     EAARVSEWEERYKLSRSEFEQFWKGLPQTVQNKLQASQKTWKSGMDKICANNAKAEGE
                     TPNGIKVSELACKTAETEARLEELHNRKKALIDEMVREEDKKELPKRL"
     gene            complement(9070..10716)
                     /gene="pgi"
                     /locus_tag="WNA25_001513"
     CDS             complement(9070..10716)
                     /gene="pgi"
                     /locus_tag="WNA25_001513"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFA9824240.1"
                     /translation="MKHLHDLPAWSKLWNHFDDSKTLHMREMFEQDPQRAERYWLQVG
                     GLTLDYSKNRINDETMSLLFELAREAGVPERMRQMFHGEKINTTENRAVLHVALRNRT
                     NSPIMVDGEDVMPKVNRVLQRMGEFAHEVRSGSWLGYTNQVITDVVNIGIGGSDLGPL
                     TMCTALKPFGHPRLNMHFVSNVDGSQLRDVLSKVHPETTLFIIASKTFTTQETLTNAL
                     TAREWFLNHAGDEEAVAKHFAAVSTNRKAVAEFGIDIANMFEFWDWVGGRYSLWSAIG
                     LPIMLYLGEENFIEMLNGAHLMDRHFINTPLERNLPVILALIGIWYINYYGGGSHVIA
                     PYDQHLHRLPKFIQQLDMESNGKQVTLDGKAVGHETSPIIWGETGINGQHAFFQLLHQ
                     GTHITPIDLIASLEKRSNLPGHHEILLANVFAQAEAFMCGKTPDEVRAELKAQGMDEA
                     RIEELVPHKIFSGNRPTNLILMDKVNPRNMGSLIAMYEHKTFVQGIIWGINSFDQWGV
                     ELGKQLAKTILGELTGETEPQKHDSSTERLINLYLQTNRK"
     gene            complement(10942..11790)
                     /gene="hexR"
                     /locus_tag="WNA25_001514"
     CDS             complement(10942..11790)
                     /gene="hexR"
                     /locus_tag="WNA25_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFA9824241.1"
                     /translation="MLSKISESLADLSGAERKVAECALAEPKWFVHAAVAEIADRASV
                     SQPTVIRFCRSLGYKGLPEFKLSLSASIGHEGMPYVHEELNADDDMANVVEKVLGNAA
                     AALLGERRFLKESELENGIATLMHARRVEFYGVGNSGIVAQDAQHKFFRFGMSTVAYV
                     DTHTQLMAASVLSDQDVLVAISNTGSSIELLDAVSIAKENGASVIALTRNDSPLAQLA
                     DCVLSVATQENAELYTPMVSRLLQLAVIDILAIGLALRLGDAASLQLQKSKKSIHNKH
                     IDYDKD"
     gene            complement(11839..12825)
                     /locus_tag="WNA25_001515"
     CDS             complement(11839..12825)
                     /locus_tag="WNA25_001515"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFA9824242.1"
                     /translation="MSSTPNKHADYPRLVADIGGTNARFALETAPCVIEKVAVLPCKE
                     YDTVTDAVRAYLNQSGATGVRHAAFAIANPILGDWVQMTNHHWAFSIETTRQALGLDT
                     LILLNDFTAQALAVTQTSSKDLMQVGGQKPVEFAPKAVIGPGTGLGVSGLVHSPAGWV
                     ALAGEGGHTSFPPFDDMEVLIWQYAKNKYRHVSAERFLSGAGLSLIYETLAAKQKAEP
                     AKLMPSEITEKALNCESPLCRQALDIFCAMLGTVASNLALTLGARGGVYLCGGIIPRM
                     LDYFKTSPFRSRFENKGRFEAYLAAIPVYVVLSEFPGIAGAAAALGNHLKNV"
     gene            complement(12806..13501)
                     /gene="pgl"
                     /locus_tag="WNA25_001516"
     CDS             complement(12806..13501)
                     /gene="pgl"
                     /locus_tag="WNA25_001516"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFA9824243.1"
                     /translation="MFVWHEYENAAEAAQSLADAVADALQGALDEKGGAVLAVSGGRS
                     PIAFFNALSQKDLDWKNVGITLADERIVPTNHADSNTGLVREYLLKNKAAAAVWIPMV
                     EDGKTETELHPDAVVDYALKHYKQPDVLILGMGNDGHTASIFPKAPQFQTAIDGSAGV
                     ALVHTTPVTAPHERISMTLDAIAHTGHVFLAIQGEEKKAVFDQAAQGENREYPISLVL
                     NHQGVNCHVFYAE"
     gene            complement(13653..15098)
                     /gene="zwf"
                     /locus_tag="WNA25_001517"
     CDS             complement(13653..15098)
                     /gene="zwf"
                     /locus_tag="WNA25_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFA9824244.1"
                     /translation="MSTQTNFDLVLFGATGDLAMRKLLPCLYQAHVAGLLHPEGRILG
                     VSRSELDTEGFLAKVETSSKIHVKENFSGEAWASFVERLAYLKVDVTQPDDFAALGDL
                     VKARKETDNVVIYLSTAPKFFAQACENLAAIGLNADNVRVVLEKPLGTDLASSQQINT
                     DVARYFKEGQIYRIDHYLGKESLQNLLALRFANVMFEPLWNNKYIESVQLTIAEQLGV
                     EERGEFYDITGALRDMVQNHLMQMLCMTAMEAPASLDADAVRDEKVKVIKSLKPLTIE
                     SVNENVVRGQYTAAKGMNGYLEEINVPQDSFTETYVAIKAEIENERWKGVPFYLRTGK
                     RMAGKVAEIVLNFRPLQNHIFDNSQTAPNRLVIELQPNESVRLYTQVKTPGAGNKVEV
                     TPLGVDLGKAVEGRRAEAYERLLLDVINGKLALFNRRDELEAAWEYVMPILENWANNT
                     TPPHGYGAHSWGPEAARELLARDGNKWHEEQ"
     gene            15416..15534
                     /locus_tag="WNA25_001518"
                     /pseudo
     CDS             15416..15534
                     /locus_tag="WNA25_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            15618..17453
                     /gene="edd"
                     /locus_tag="WNA25_001519"
     CDS             15618..17453
                     /gene="edd"
                     /locus_tag="WNA25_001519"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFA9824245.1"
                     /translation="MNHTPIHPKLAEITGRIIERSRPTREKYLAKIRSAKQMGRLERN
                     QLGCSNLAHGYAAMPKSIKIEMLQETVPNLGIITAYNDMVSAHQPFKDFPDQIKDEAQ
                     KNGATAQVAGGTPAMCDGITQGYAGMELSLFSRDVIAMSTAVGLSHQMFDGSLFMGVC
                     DKIVPGLMIGALSFGHIPGIFVPAGPMSSGIGNKEKARTRQLFAEGKVGRDELLKSEM
                     GSYHSPGTCTFYGTANSNQMMMEMMGVHLPAAAFVHPYTDLREALTRYAAGHLARGIK
                     NGTIKPLGEMLTEKSFINALIGLMATGGSTNHTMHLVAMARAAGVILNWDDFDEISSI
                     IPLLIRVYPNGKADVNHFTAAGGLPFVIRELLDAGLLHDDVDTVVGHGMRHYTKEPFL
                     IDGKLEWREAPETSGNDDILRKADNPFSPDGGLRLMKGNIGRGVVKVSAVREGCRIIE
                     APAIVFNDQREVLAAFERGELERDFICVVRYQGPRANGMPELHKLTPPLGILQDRGFK
                     VALLTDGRMSGASGKVPASIHMTPEALMGGNIAKIRTGDLIRFDSVTGELNVLINEAE
                     WNVREVERIDLGANQQGCGRELFAGFRSMTSSAETGAMSFGGEFA"
BASE COUNT         4217 a         5302 c         4492 g         3593 t
ORIGIN      
        1 caaaaaatat gcgaaacaaa ataaaccgca acgacatgga actcggctac acgccttaca
       61 accttcgcac cctgcgcaac cgctgcaaac tgacacaggc cgagttggcg caaatcgtcg
      121 gggtgaagca ctacatacag gtaggccgct gggaagccga acccgatacc gaaacgcggc
      181 gggccgatat gccgctggaa aaatggcggc agttcctcga ttggatagaa aaaacaaacg
      241 ccgtctgaaa ccttcagacg acatttttta cccaccccca aacctaaaaa ccacaaccaa
      301 gccctacacc gcttgccccc acctcgccgc cgccccaaaa taacctcact ctgttggaag
      361 cctgccggaa acggcaaaag ccgccccgtt ccgccattac ggggcggttt cccacatcaa
      421 gacgagacag aaatgaccga ccgccaaata gacaacctga tcaaacccgc caccatcgcc
      481 gacaccgacc ccgcgtccgc gcacaacacg gcggcattac caccgactgg ctgccctata
      541 tcgtcccctt tgcaggcggc gtgtcagtat ggcgcatccc aagtgtcggc gaagcctgca
      601 ccatcttatc ccctgccggc gaacccgaga acggcgtagt tttatgctgc caagcctccg
      661 accgataccc cgccccgtcc gccgaccccg ccgaaaccgt cgtccgattt cccgacggca
      721 cgcacatccg ctacaaccac aacagcggcg cgatggaatt aaaagccgtt acaagcctga
      781 ccatcgacac cccccaaacc accataaccg gacacctgac cgtcaaccaa accaccaccg
      841 cccaagggct gctgacctac caaaatggca tgaacggcca aggcggcagc ctgtccgagc
      901 acacccaccc cgacgactcc ggcggtacaa cggaaaaacc ccaatgaccg acgcagaaaa
      961 cggacgcggg caagacaccc tcgcccacat cgcccaatcc atccgcaaca tcctgttcac
     1021 aagaatagga acgcggctga tgcgcgaaga atacggcagt tttattcccg acttgatcga
     1081 tatgcccgcc ggtcacgccg ccatcgccct catccaccaa gccgccgtta ccgccctcgc
     1141 aagatggaaa ccccgcataa ccgtccgccg catacaggca gacaccgccg acctcgccgc
     1201 cggcaaaatc aaattaaccc tagacgtaac ccttgccgac ggcggcgaac gaacctaccg
     1261 catcaaataa taaaaacaac aggataaaca atgggaaaca gccgattaag ccaactcccc
     1321 gcgcccgccg ccatcgaaga aaccgacttt gagggcatct tcgcgcgcaa aaaagccgcc
     1381 ctcaccgccc tatgtcccga aagcatccgc gaaaccgtcg cccaaaccct cgaattagaa
     1441 tcagaacccc taaccatcga cctgcaacag caagcctatc aagagctgct cgtccgcaac
     1501 cgcatcaacg aagccgtcaa agccaacctc ttggcatacg cacaaggcag cgacctcgac
     1561 cacatcgccg cccaatacgg actttcacgc aaaaccatcc gcgccgccga ccccgacgcg
     1621 aacccgccgg ttgccgccga atacgaaacc gacgacgcat tccgcgcccg cgtccaagcc
     1681 catcccgaaa aatacgccgc cgggccgcgc accgcatacg aagcccacgc catcgacgca
     1741 cacccccaaa tcacacacgc ccgcgccgtg cgccgcgccg ccggcacggt ggaggtttac
     1801 atcaaaaccc aaagcggcac gcccgacgaa accattttga ccgccgcgcg cgaatacctg
     1861 tccgccgaaa cacgccgccc cctttgcgac aacgtgcaag taaccgccgc ccaacccaaa
     1921 gacgcggcag tagagtattc cgccgaatac caccccgccg ccaatattca agccgaacgg
     1981 cagaccgcct gcgaagcctt ggataaccta tggcggcaaa acgcccacat cggcgcatcc
     2041 gtcgccctgt ctaaaatcat cggcgcgtta gacacccccg gcgtgaaaaa aaatcacact
     2101 gcacagcccc gccgccgaca tcgaatgcgg caacggcgaa tacatccgaa tcacgtccac
     2161 gctcgagcgc gaataaatga acagcaccgt ccccgcgaac aacagccccc tgcaacacgc
     2221 actggcaaag ctgacagaac gcgaaactgc cgccgtctcc cgccaactcg accccggatt
     2281 tttacccttt cacgccttcg caagaagcat cggcacggaa gagggctggg actttgccga
     2341 aactgacgaa gcccgccgca acctcatcgc aggctttgcc gaaatccacg cccgaaaagg
     2401 cacgccgtac gccatccgcg ccctcttccc catcttgcgg ctgggcgaaa tccaaattat
     2461 cgaacgcgac ggcgagttca agtgggacgg ctcggtcttg ttcgacggca gccgcacatt
     2521 cggcaggcgc aagggtgact gggcggaata ccgcattgtc ttaacgcgcc ccgtcagcat
     2581 ccgccaaacc gcccgcatcc gcgccatgtt ggcggaaatc gcccccttgc ggtgcgaact
     2641 taccgcgctc gactaccgca accatcccca ccgctggaac ggcaaaatcc gctttaacgg
     2701 cgaatacggt ttcggcacga cataacgcgc ccccgaaaat caacaaacaa aaggaagccc
     2761 caaaatggca aacgcaaccg aacaaaacca attcgaccaa gccgtccgcc tcatcgaacc
     2821 cggcgacagc gtcgtcgtcg gcccaggcgc gcccgtcaac caaccactgc aagccctcgc
     2881 caaccgcacc cttttgctca aaaaccaaac cgaagccctt cagacggcat ccgacaccaa
     2941 agccgcacca tcgcactggg cgcgcccggg caaatcaccg caacaagcca aaacaccgtc
     3001 ccaaaaaacg gccacaccca cgccatcgac accgcacgca ccgaccgcgc cggcatcgtc
     3061 cgattagaca atgccattag cgaagccgaa gacaccgccg ccacccccaa agccgtcaaa
     3121 accgcgatcg accaagcccg cgccgccgcc gcgaccgccg acctcaaagt ctccctctcc
     3181 gacaaccaaa ccgttaccgg ccaaaaaacc tttaccgccg aaacacaatt ccaaagcggc
     3241 atccggctgt ccgccaaccc gacgcactga aacggcggct acaaagccta tatcggcgcg
     3301 gataacgaaa acgcccacat cgtcttcggc gacgacaccc tgcgcctgca cggcgcaaat
     3361 aaccgcattt cctacaacaa ccacgacatc ttccacaaag ccaacaaacc gcgtttcaac
     3421 gaagacatcg aaggcaaacc gaacacgctg tccggctacg gcatcggcaa cttcaaagtc
     3481 gaaacattcc ggggcgattt gaacagcctc aaaaccgacg gcgtctattc cctgccgacg
     3541 gcggtcggca gctcgaacct gcccgttgaa aacaccgcct gccatatcca agtcatcgcc
     3601 ggcacgcaac ccggctggtg caggcagttg ggctatcccg cctacacgtc cgacgtgtac
     3661 gaacgctacc agaccagcag cgcaaacgac gactggagcg cgtggaaaaa actcaattcg
     3721 gaaggcatcc ccgccggcgc gatcgtgtcc tttcccaaag ccgtccgaaa ccccgcaggc
     3781 tatctcaaag ccgacggcac gatctttgca caaaacacct tctcgacctt taccgcgccc
     3841 tgggcaacac aaacaaactg cccgacctga cccgtaccga catcggcatc accgcgtggt
     3901 ttccgtccga ccaaatccag acgggctggc tggcgtttga cgacatccga gagcgcgtaa
     3961 ccgaatcggc ttatcccgaa ctttaccgcc tgctgaccga aaaatacgga cgcatccaaa
     4021 acgtcccgca ggcggaagac cgctttatcc tcaacgcggg caacggcttg gcggtcggaa
     4081 cgaagcagga agacgaaatc aaacggcacg tccacaaagt attttcacac tgggcaaacc
     4141 acccagacgc cgccgccgtc ggttacgaag accgcaatga aaggcaaaga agcgcgctcg
     4201 tatcgacttg gacggacggc gacttaaacg acaacggctt tttaaccccg cgattggaca
     4261 gcaaaatggc aacgggcggc gcagaaaacc gccccaaagc cctggtttta aaactgtgca
     4321 tcaaagccgc cgacaccttg ggcgaagccg tgttccggat aaagtcccac ggcgaaaccg
     4381 ccaacgccgg cgcactggac gcgtcaaggc tggcgcaagg tttgcaggaa aaagccgacc
     4441 gcgaccacac ccacaccacc gcccaaatcc aagggctgga cgaaaaaatc agcgccgccg
     4501 ttgccgcgca attcacacgc caaaccatcg gcggcgtgga tattgtcaga ttccccgacg
     4561 gcacaatgat acagaccggc agttacaggt ttgcaagagg cggcagcccc ataggaaacg
     4621 aagttgtctt ccccatcgcc tttgccgacg gcaacgtcaa atgcttcgta tccgaacgcc
     4681 attcgggacg cgtcaacggc gaaaggcaac acaactggct gtttatccgc gcaaaaaacc
     4741 acgccgccgc cattattacc aactggtacg aaagcagttg cgactggatg gccatcggca
     4801 aatccgcctc gggaaacgcc gccagcccca ccccgatagt ccccgaaata cctgaaatcg
     4861 atgaagaacc gcaaagagag agtggaagat catcaaccgg actccgaaac ccccgccgcc
     4921 accgaggcct cgacttcccc gtggggtcgt aagactagcg ggcggcaggg caggctgtag
     4981 agacgggctg tagagacggg cttcagccca ccgttccaag caatccgacc gaaaccggcc
     5041 gcgccgccaa tcctgcgaaa cctatgcccc gccaatcctg ccactcttcg ccattcccgc
     5101 gaaagcggga atccggaacc caaacgcggc aggaatctat cggaaagaat aacccgaccg
     5161 ccgtcattcc cgcgcaggct ggaatccaga cccacaacgt taaggcggtt tatcagaaaa
     5221 aaccgaaacc gaacgcccta gatttccgcc tgcgcgggaa tgacgaagag ttggggagtg
     5281 acgaaaggcg ggaacaaccg cgcaaaaagc cgccgactcc ttcagacggc atcggcaata
     5341 aaaaccgcac ggccgaaacc gcgcgggaaa ggatagccgg gcgcgcccga taagcagcgg
     5401 ccgcagggtt aagtccttgc acctgttttc atcgaagaca aaggcggttc gggaaataag
     5461 ggcgcaatat caaaagtttt gcgttgcctg atttgccaca aatcataacc ggcctgcata
     5521 ttcagccaaa aatcaggcga agtgttgagc agttggctta aacgaaccgc catctcgggc
     5581 gtaatcgaag attgggcgtt gacaatgcgc gataaggcaa cccggctgac acccaggcgt
     5641 ttcgctatat cggtaatgct tgcgccgtta atatactctt ttaaaacaag gcccgggtgg
     5701 gcaggattgt gcattttcat attattccta gtgataatcc tgataatcga caacttcgac
     5761 attgccgtcg ttgaaacgaa aagtcaaacg ccaatttccg ttgactttga accgcccaat
     5821 gtcccgacaa actgcccttg aggggatgaa gattccaact cggcacagcc atatccgaag
     5881 gcgtttgcgc ggcattcagc gcggttagaa gcagattcag tttgacggaa tgcccggctt
     5941 ggatgcccga tagcgagcct gttttaaaaa agcgttcaag ccctttgtgt ttgaaagaaa
     6001 caatcatcat cccaccccgt ctgtatcgcc aaaggataca aaacttttat ttgattgcaa
     6061 agcgacgata tacaaaaaag gaaaccccga aatgaccatc tatttcaaaa acggctttta
     6121 cgacgacaca ttgggcagcg tccccgaagg cgcggttgcc gtcggcggcg aagagtacgc
     6181 cgccctcttc gcaggacagg cgcagggcgg gcaaattgcc gccgattccg acggccgccc
     6241 cgttttaacc ccgccgcgcc cgtccgagta ccacgaatgg gacggcaaaa aatggaaaat
     6301 cagcaaagcc gccgcccgtt tcgccaactc ccgtttcgcc aaacaaaaaa ccgccttggc
     6361 attcagcctc gcggaaaagg cggacgaact caaaacagcc tcttggcggg ctatccccaa
     6421 gtggaaatcg acagctttta caggcaggaa aaagaaaccc tcgcgtggca ggcggacaac
     6481 gacgccccga ccccgatgct ggcgcaaatc gccgccgcaa ggggcataga attggacgtt
     6541 ttgattggaa aggttgtcga aaaacccgcc cgcctggccg ttgccgtcga cgcgattatc
     6601 ggaaagcggc agcaacttga agatagatta aataccatcg aaaccgcgcc agcattagat
     6661 gcgttagaaa gggaaatcaa agaatgggcg ctaaacgtcg gctgagagaa tatatttatc
     6721 atctactagt ggccatagac caactgttca acgctataac cggcggcgcg gcagacgaaa
     6781 ccctctcaag tcgcacctat cgcggcgcgc ggctcgccca acaacccaaa acccgctgga
     6841 agattttata gtggattaac aaaaatcagg acaaggcgac gaagccgcag acagtacaaa
     6901 tagtacggca aggcgaggta acgccgtacc ggtttttgtt aatccactat atataccctg
     6961 atcaacggcg tatttttcga ccgccaccac tgccggcagg cgtatatcag ctaactgaaa
     7021 ggcaggcagc acgacgcgcg gttcaaccaa agccgcgccg ccaatgaaag ggatacgcgg
     7081 taaaattgcc gccgcgccgc attcagacgg catcggcaat aaaaaagccc gaaaaaatcg
     7141 ggctgaaagt taaggctgca tcgcctttaa ggcggctttc gccaaaaaac cgctcgggtt
     7201 tcatgacggg cggcagtgca ggcatcgaca cggttgagga tatgttgcgg ccaactgacg
     7261 ttaaaccgca cctgtccggt taatgcggca tcgtccgctt caaccatcgt ccacaacacc
     7321 gcatcggcat aatcgggatt gtcccgatta acctctatgc tttgcgcctc gggaatcggc
     7381 aggttttcct gcaaaagccc ttcgagatgg aacattgcgg cggctttggc atcggcaagc
     7441 gcgtcggcaa ccgtatcgcc gacaggatag cagcccggca gatcgggaat aatcacgccc
     7501 cagttgcttt gatcggggtc ttggaacagc acggcgggat agtaaatcat tacttccttt
     7561 ctcggatatg cggtatcggc aaagtccaaa cgcaacgaag gggatttaaa aacgacaaaa
     7621 acgcaaaagc cgccgacatt cccgcatcca agtttcagtc aatcagataa ccttggattt
     7681 ctttggtttt cgcattgatt tctctggtac ggcagtcaga ttgtgccacc ccgtattcgt
     7741 cgccgtcggc gcatttggca tccaaaccgc tttgccagtt gcggtactgg ggcataacgg
     7801 tttcggcaat atcggcgggc agtgcctgaa ccgcgctgtt cagagccgct ttggcagttc
     7861 tttcttgtcc tcttccctga ccatttcgtc gataagggct tttttacggt tgtgcagctc
     7921 ttccaagcgt gcttcggttt ctgccgtttt acacgccaac tcactgactt ttatgccgtt
     7981 tggcgtttca ccttcggctt tcgcattgtt ggcacagatc ttgtccatac cgcttttcca
     8041 tgttttctgc gaggcttgca gcttattctg tacagtttga ggcaatcctt tccaaaactg
     8101 ctcgaactcg ctgcgcgaca gcttgtagcg ttcttcccat tcggatacgc gggcggcttc
     8161 ctgctcccga cccaatgcct cctgcgccgc cgcttcttct gcctccctcg cttcctgcgc
     8221 ggtggcttct gccgcttcaa gtttttcgtt cctttgtttc gctttgtcca acggctcttt
     8281 aatcagtgcc atagacacca gctcgctgag cggatggatg atatcgtgtg cctgactgag
     8341 ttcggcaaag attttgcttt tgtcgtctgt cggctggacg ctgtaagaaa tagttttgac
     8401 ataatacgcg ccttctttgc ggtagtaggg ttcaaaaaag tcgggcgttt tcttatagct
     8461 gttgcctata gattggttgg cggcgacggc ataatcgaca acatcgtcgg gcacgtccag
     8521 ttttaacgcg gcttcacatt tgagtttgct ggacgtgtcg gttttctcgg tagttcggac
     8581 ttcatcgacg gttatgccca aacgttcgac cattctgcgg atatgcgata tgccgatatc
     8641 ggcaaatgct tggtctttga atgcgtcgtc gtcaaaagtt ttgacggttt caccttcgac
     8701 attgtcgcgg accagtttga ccaaaatctc tttagtctct tcgcttccgc aggaaatttc
     8761 ctttccggaa aacgggttgc ctattcccaa acggtcgcag cccgacaaaa tcagcatact
     8821 tgccaatgcc agtgcgggta aatttttttt cattagattt cctttgccgt ccaaacacgg
     8881 actgtctgtt gcgtatttcc acacacgcta cggcacttga agtacatggc tgcggaacat
     8941 atatgtttaa atttatgcaa accgccatat cgcgattgca cacgccctgc aaatttaccg
     9001 acggttttcc tgttcgataa aaatgccgtc tgaaacggtc ggcgttcaga cggcattttt
     9061 ccgcaggttt tatttgcggt tggtctgcag gtagaggtta atcaggcgtt cggtcgagct
     9121 gtcgtgtttt tgaggttcgg tttcgcctgt cagttcgccc aaaatggttt tagccagttg
     9181 tttgccgagt tccacgcccc actggtcgaa gctgttgatg ccccaaatga tgccttggac
     9241 gaaggttttg tgttcgtaca tggcaatcag gctgcccata ttgcgggggt tgaccttatc
     9301 catcaggatg aggttggtcg ggcggttgcc ggagaagatt ttgtgcggaa ccaactcttc
     9361 gatgcgcgcc tcatccatac cctgcgcttt gagttcggcg cggacttcgt cgggggtttt
     9421 gccgcacata aaggcttctg cttgtgcaaa gacgttggca agcaggattt cgtggtgtcc
     9481 gggcaggttg ctgcgttttt caagcgaggc aatcaggtcg atgggggtga tgtgcgtgcc
     9541 ttgatgcagc agttggaaga aggcgtgctg accgttaatg cccgtttctc cccagataat
     9601 cggcgaggtt tcgtgtccga ctgctttgcc gtccaacgta acctgtttgc cgttgctttc
     9661 catatcgagc tgctggatga atttgggcag gcggtgcaaa tgttggtcgt aaggcgcgat
     9721 gacgtggctg ccgccaccgt agtagttgat ataccagatg ccgatgaggg cgaggatgac
     9781 gggcaggttg cgctcgagcg gggtgttgat gaagtgtcgg tccatcaggt gcgcgccgtt
     9841 gagcatttcg atgaagtttt cttcgccgag atacagcata atcggcaatc cgatggcgga
     9901 ccacaggctg taccgaccgc cgacccaatc ccaaaattca aacatattgg cgatgtcgat
     9961 gccgaattcg gcgacggctt ttcgattggt ggaaacggcg gcgaagtgtt tggcaacggc
    10021 ttcttcgtca cccgcatgat tcaaaaacca ttcgcgcgcg gttagcgcgt tggtcagcgt
    10081 ttcctgcgtg gtaaatgttt tggaggcgat gatgaacagc gtggtttccg ggtggacttt
    10141 ggacaatacg tcgcgcagtt gcgagccgtc cacgttggag acgaagtgca tattgaggcg
    10201 cggatgaccg aaaggtttga gcgcggtaca catcgtcagc gggcccaaat ccgatccgcc
    10261 gatgccgatg ttgacaacgt cggtaatgac ttggttggta tagcccagcc agcttccgct
    10321 gcggacttcg tgtgcaaatt cgcccatacg ttgcaaaacg cggttgactt tgggcatcac
    10381 atcttcaccg tcaaccataa tcggcgaatt ggtgcggttg cgaagggcga catgcaggac
    10441 ggcgcggttt tcggtggtat tgattttttc gccgtggaac atctgccgca tccgctccgg
    10501 cacgcctgct tcacgcgcca actcgaacag tagggacatg gtttcgtcgt tgatgcggtt
    10561 tttggagtag tccagcgtca gtccgccgac ttgcagccag tagcgttccg cccgctgcgg
    10621 gtcttgctcg aacatttcgc gcatatgcaa tgttttgctg tcgtcaaagt ggttccacaa
    10681 ttttgaccag gccggtaaat cgtgaaggtg tttcatctgt atgctcccga ttgaggtttt
    10741 ccgtgacggg gggcgtttcc gcacatcgat gcgcacataa ggcgcaaccc tgcgtaccgc
    10801 cccgatttgg aaggggttgc ggcaaacccg aaccctgccg ccttaaaggg cggggtttcc
    10861 gccattaagg aaattctgat aaaaggcttg ccggagcctg tccggccgtt cagacggcat
    10921 tggcgggatg ccgtctgaac atcagtcttt gtcgtaatcg atgtgcttgt tgtgtatgct
    10981 ttttttgctt ttctgcaatt gcaggctggc agcatcgccc aagcgcaggg caagtccgat
    11041 ggcgagaata tcgatgacgg caagctgcaa gaggcgggaa accatgggcg tgtagagttc
    11101 ggcgttttcc tgtgtggcaa cgctcaacac gcagtcggca agttgcgcca gaggcgaatc
    11161 gttgcgggtc agtgcgatga cggacgcgcc gttttctttg gcgatgctga ccgcatccaa
    11221 aagttcgata gacgaacccg tgttggaaat ggcaaccaaa acatcctgat cgctcaaaac
    11281 ggatgccgcc atcagttgcg tgtgcgtatc gacataggcg acggtggaca tgccgaaacg
    11341 gaaaaatttg tgctgcgcgt cctgtgccac aatgccggaa ttgcccacgc cgtaaaattc
    11401 gacgcggcgg gcgtgcatca gggtggcgat gccgttttcg agttcggatt cttttaagaa
    11461 cctcctctcc cccaacaagg ctgcggcggc gttgcccaac actttttcaa ccacattcgc
    11521 catatcgtcg tcggcattga gttcttcgtg gacataaggc ataccttcgt gcccgatgct
    11581 ggcggacagg gagagcttga attccggcag ccccttataa ccgaggctgc ggcagaaccg
    11641 gatgacggtc ggctggctga ccgaggcgcg gtcggcgatt tcggcgacgg cggcgtggac
    11701 aaaccacttg ggttctgcca aggcgcattc ggcaactttg cgttccgcac cggacaggtc
    11761 ggccagtgat tcgctgattt tgcttaacat aatgatatgc ccttcgataa tgcggccctg
    11821 ctgcaagggc tgccgtggtt agacgttttt caggtggttg ccgagcgccg ccgccgcgcc
    11881 ggcaataccc ggaaattcgc tcaagacgac ataaacggga atcgcggcaa ggtaggcttc
    11941 aaaacgccct ttgttttcaa agcggctgcg gaagggggag gttttgaagt aatccaacat
    12001 tcgcgggata atgccgccgc acaaatacac gcccccgcgc gcgcccaaag tcagggcgag
    12061 gttggaagcg accgtgccca gcatcgcgca gaaaatgtcc aatgcctgcc ggcacagggg
    12121 cgattcgcaa tttaatgcct tttcggtgat ttcagacggc atcagttttg ccggttcggc
    12181 tttctgcttg gccgccagcg tttcataaat caggctcaaa cctgcgccgc tgaggaaacg
    12241 ttcggcggaa acgtggcggt atttgttttt ggcgtactgc caaatcaaca cttccatatc
    12301 gtcaaacggc gggaaactgg tatgcccgcc ctcgcccgcc aacgccaccc agcctgcggg
    12361 gctgtgcacc aatccgctca cgcccaagcc ggtaccgggg ccgataacgg ccttgggggc
    12421 gaattcgaca ggcttttgcc cgcctacctg catcaggtct ttgcttgaag tctgcgttac
    12481 cgccaatgcc tgcgcggtaa agtcgttcaa aaggatgagg gtgtccagcc ccaaagcctg
    12541 acgggtggtt tcgatggaaa acgcccaatg gtggttggtc atctgcaccc agtcgcccaa
    12601 aatcgggttg gcgatggcaa atgccgcgtg ccgtacgccc gttgcaccac tttggttcag
    12661 ataggcacgc accgcgtcgg taaccgtgtc gtattcctta cacggcagga cggcgacttt
    12721 ctcaatcacg cacggcgcgg tttccaatgc gaaacgggcg tttgtgccgc cgatgtcggc
    12781 aaccagcctc gggtaatcgg cgtgtttatt cggcgtagaa gacatggcag ttcactcctt
    12841 gatggttcaa aacgaggctg atcggatatt cgcggttttc gccttgtgcg gcttggtcga
    12901 acacggcttt tttctcttcg ccctgtatcg ccaaaaatac atgcccagta tgggcaatcg
    12961 catccaaagt catgctgatg cgctcgtgcg gcgcggtaac gggcgtggta tgcaccagcg
    13021 cgacacctgc cgaaccgtcg attgccgtct gaaactgcgg agcttttggg aaaatcgaag
    13081 ccgtatgccc gtcgtttccc atacccaaaa tcaaaacatc gggctgcttg taatgtttca
    13141 gcgcataatc gacaacagca tcgggatgta attcggtttc agtttttccg tcttccacca
    13201 taggaatcca cactgccgca gcggctttgt tctttaacag gtattcgcgc accagaccgg
    13261 tattgctgtc ggcgtggttg gtcggcacga tgcgttcatc tgccaaggta attccgacgt
    13321 ttttccaatc caaatctttt tgcgacaggg cgttgaaaaa tgcaatcggc gaacgtccgc
    13381 cggaaactgc caacaccgca ccgcccttct cgtccagtgc gccctgcaaa gcatccgcca
    13441 ctgcgtcagc caaagactgc gccgcttctg ccgcattttc gtattcgtgc caaacaaaca
    13501 tatttgtgtc cttttgtgat ttcagacggc atatcccgtt acagggcggc tgccgggcaa
    13561 tggcttagcc gcttccgcct cgaagcggga ctgctttttc tgccccgccc tccttcagac
    13621 ggcattgcga acccgatgcc gtctgaagcg cattactgtt cttcgtgcca cttgttgccg
    13681 tcgcgcgcca acagttcgcg ggcggcttca ggaccccaag agtgtgcgcc gtaaccgtgc
    13741 ggcggcgtgg tgttatttgc ccagttttcc aaaatcggca tcacatattc ccacgcggct
    13801 tcgagttcgt cgcggcggtt gaacaaagcg agtttgccgt taatcacatc caacagcagg
    13861 cgttcgtaag cctcggcgcg gcggccttca acggccttgc ccaaatccac gcccaaaggc
    13921 gttacttcca ctttgttgcc cgcacccggg gttttcacct gcgtgtaaag gcggacggat
    13981 tcattcggtt gaagctcgat aaccaaacgg ttcggcgcgg tttggctgtt gtcgaaaata
    14041 tggttttgca gcgggcggaa gttcaaaacg atttccgcca ctttgcccgc catgcgtttg
    14101 ccggtacgca ggtagaaggg aacgcccttc cagcgttcgt tttcgatttc ggctttaatg
    14161 gcgacgtagg tttcggtaaa gctgtcttgc ggaacgttga tttcttcaag atagccgttc
    14221 atgcctttgg cagcggtata ttgtccgcgc acgacatttt cattgacaga ttcgatggtc
    14281 agcggcttca atgacttgat gactttgact ttttcatcgc gcaccgcgtc ggcatccaag
    14341 ctggcggggg cttccatcgc agtcatgcac agcatctgca tcaaatggtt ttgcaccata
    14401 tcgcgcaacg cgccggtaat gtcgtaaaac tcgccgcgct cttccacacc gagctgttcg
    14461 gcgatggtca gctgcacgct ttcgatgtat ttattgttcc acagcggctc gaacattaca
    14521 ttggcaaaac gcagcgcaag caggttttgc aggctttctt tgccaaggta gtggtcgatg
    14581 cggtaaattt gcccttcttt gaaataacgc gccacatcgg tattgatttg ttgggaagaa
    14641 gccaaatccg tacccagcgg tttttccaaa accacgcgca cattgtcggc attcaaaccg
    14701 atcgcagcaa ggttttcgca ggcttgcgcg aagaatttcg gcgcggtgga cagatagatg
    14761 acgacgttgt cggtttcttt gcgtgctttg accaaatcgc ccaaagcggc aaaatcgtcc
    14821 ggctgcgtaa catcgacttt gagatatgcg agacgttcaa caaacgaagc ccacgcctca
    14881 ccggaaaaat tttctttcac atggattttg gaactggttt ccaccttcgc cagaaaacct
    14941 tcggtatcca actcgctgcg gctgaccccc aaaatacgcc cttcgggatg aagcagaccg
    15001 gcaacatgcg cctgatacag gcagggcaaa agtttgcgca ttgccaaatc gccggtcgca
    15061 ccgaacaaca ccaaatcaaa atttgtctgt gtactcatcg cattatctcg tcaggaaagg
    15121 attttttcga tgccgtctga aacctgtttt ttccatcacg cagcatcgca atatcggaaa
    15181 caaaggcagg cggcataatg agtagtaata ctacacaccg ctacactttt tgtctattcc
    15241 catttttaca atttatttga cctagtccaa aaatcaggca ggtttcccct attccgttac
    15301 aacaatcaga agattctgcg atttaaatca aatttctttt caatgtctga tttttttgta
    15361 acaaaattac aaattttgta ctataatagc acccgcttcc cactttcaga cggcgtacct
    15421 tttaaaatat agtgaattaa atttaaatca ggacaaggcg gcgagccgca gacagcacaa
    15481 atagtacggc aaggcgaggc aacgccggac cggtttaaat ttaatccact atactcaccg
    15541 tctaacatac ccgatacaaa aatcagaaac gcacaaacaa atccccaata ccaccccgtt
    15601 ccgccaggag accgaacatg aaccacactc ctatccaccc caaactcgcc gaaatcaccg
    15661 ggcgcattat cgaacgcagc cgtccgacgc gtgaaaaata tctggcgaaa atccgcagtg
    15721 ccaaacaaat gggacgctta gagcgcaacc agctcggctg cagcaatttg gcgcacggtt
    15781 atgccgccat gcccaaaagc atcaaaatcg aaatgcttca ggaaaccgtc cccaacttag
    15841 gcatcatcac tgcctacaac gacatggttt ccgcacacca gccgtttaaa gacttccctg
    15901 accaaatcaa agacgaagca caaaaaaacg gcgcgaccgc acaagtcgcc ggcggcacgc
    15961 ctgccatgtg cgacggcatc acgcaaggct acgccggcat ggaattgtcg ctgttttccc
    16021 gcgacgtgat tgcgatgagt accgccgtcg ggctgtcgca ccaaatgttt gacggcagcc
    16081 tgtttatggg cgtgtgcgac aaaatcgttc cgggtctgat gataggtgcg ctttcgttcg
    16141 gtcatattcc cggtatcttc gttccggcag gcccgatgtc cagcggcatc ggcaataaag
    16201 aaaaagcccg tacccgccaa cttttcgccg aaggcaaagt cggacgcgac gaacttttga
    16261 aaagcgaaat gggttcttac cacagcccgg gcacctgcac tttctacggc acggcaaact
    16321 ccaaccaaat gatgatggaa atgatgggcg tacacctgcc tgccgccgcc tttgtccacc
    16381 cctataccga cctgcgcgaa gccctgaccc gctacgccgc cggacacctc gcgcgcggca
    16441 tcaaaaacgg cacgattaaa cctttgggcg aaatgttgac cgaaaaatcc ttcatcaatg
    16501 ccctgatcgg tttgatggcg accggcggct cgaccaacca caccatgcac ctcgtcgcta
    16561 tggcgcgcgc cgcaggcgtg attttgaact gggacgactt cgacgaaatt tcttccatca
    16621 tcccgctgct catccgcgtg tatcccaacg gcaaagccga cgtgaaccac tttaccgcag
    16681 caggcggcct gcctttcgtt atccgcgaat tgctggacgc aggcttattg cacgacgatg
    16741 tcgataccgt cgtcggacac ggtatgcgcc actacactaa agagcctttc ctcatcgacg
    16801 gcaaactcga atggcgcgaa gcccccgaga ccagcggcaa cgacgacatc ctgcgtaaag
    16861 ccgacaatcc gttctccccg gacggcggtc tgcgcctgat gaaaggcaac atcggacgcg
    16921 gcgtggttaa agtgtcagcc gtacgcgaag gctgccgcat catcgaagca cccgccatcg
    16981 tgttcaacga ccagcgcgaa gtgttggcgg catttgaacg cggcgagttg gaacgcgatt
    17041 ttatctgcgt cgtccgctac caaggcccgc gtgccaacgg tatgcccgaa ttgcacaaac
    17101 tgaccccgcc tttgggcatc ctgcaagacc gcggcttcaa agtggcgctg ctgaccgacg
    17161 gccgtatgtc cggcgcgtca ggcaaagtgc ccgcgtccat ccacatgacg cccgaagccc
    17221 tgatgggcgg caacatcgcc aaaatccgta ccggcgacct gattcgcttc gactccgtta
    17281 ccggcgaact caacgtcctg attaacgagg ccgaatggaa cgtccgagaa gtcgagcgca
    17341 tcgatttggg cgcgaaccaa caaggctgcg gccgcgaact cttcgccggc ttccgcagta
    17401 tgaccagcag tgcggaaacc ggtgcgatga gcttcggcgg agaatttgcc tgatgcgcgt
    17461 ttcagacggc cttttaaaac agaaggccgt ctgaaaaatt attgaagccg tttaaaatag
    17521 atgcagacgt agcgtgagct ttgcccgcga acaaaaccca agcaacatca gaatgtttta
    17581 ccccaaaggt tgtctgaaaa ctta
//
LOCUS       DAWTQS010000049        16554 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.049, whole genome shotgun sequence.
ACCESSION   DAWTQS010000049 DAWTQS010000000
VERSION     DAWTQS010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16554
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.049"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            175..2202
                     /locus_tag="WNA25_001520"
     CDS             175..2202
                     /locus_tag="WNA25_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HFA9824246.1"
                     /translation="MSLSEFIERRTSFNPMVILTTLFFVCVLVVLVLTVPDQVQMWLD
                     RAKEVIFTEFSWFYVLTFSIFLGFLLILSVSGLGNIRLGRDEDVPEFGFLSWLAMLFA
                     AGMGVGLMFFGVAEPLMHYFSDITVGAPEHRQQQALLHTVFHWGVHAWSVYGTIALAL
                     AYFGFRYKLPLALRSCFYPLLKEKISGRFGDAIDIMALLATFFGIITTLGFGASQLGA
                     GLQEMGWIAENSFGVQVLIIAAVMSLAVVSAISGVGKGVKVLSELNLGLAFLLLFFVL
                     AAGPTVYLLSAFGDNIGNYLGNLVRLSLKTYAYEREHKPWFESWTVLYWAWWCSWAPF
                     VGLFIARISKGRTIREFVFGVLLIPGLFGVLWFTVFGNTAIWLNDGVAGGMLEKMTSS
                     PETLLFKFFNYLPLPELTSIVSLLVISLFFVTSADSGIYVLNNITSRDKGLSAPRWQA
                     VMWGVLMSAVAVLLMRSGGLGNLQSMTLIVSLPFALLMLIMCFSLWKGLSADKKYFET
                     RVNPTSVFWTGGKWKERLVRIMSQTQEQDILKFLKHTASPAMHELQRELSEEYGLSVR
                     VDKMFHQDEPAIEFVIRKETMRDFMYGIKSVGQDVSDQLINDGKLPHIRHQTTYKPYA
                     YFFDGRVGYDVQYMNKDELIADILKNYERYLMLLDDVGQELMAHEQVELAE"
     gene            complement(2291..3844)
                     /locus_tag="WNA25_001521"
     CDS             complement(2291..3844)
                     /locus_tag="WNA25_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HFA9824247.1"
                     /translation="MNRTYANFYEMLAAACRKNGNGTAVFDGKEKTAYRALKQEAEAV
                     AAYLQNIGVKFGDTVALAVSNSTEFITAYFAVSAIGAVAVPMNTFLKNSEYAYILNDC
                     KARFLFASAGLSKELAGLKAQTPVEKIIWTDKSRPAGETAEGDAFFENVRRFPEKPDL
                     DRQPRINDLAHIIYTSGTTGHPKGALISYANLFANLNGIERIFKISKRDRFIVFLPMF
                     HSFTLTAMVLLPIYMACSIILVKSVFPFSNVLKQALLKRATVFLGVPAIYTAMSKAKI
                     PWYFRWFNRIRLFISGGAPLAEQTILDFKAKFPRAKLLEGYGLSEASPVVAVNTPERQ
                     KARSVGIPLPGLEAKAVDEELVEVPRGEVGELIVRGGSVMRGYLNMPAATDETIVNGW
                     LKTGDFVTIDEDGFIFIVDRKKDLIISKGQNVYPREIEEEIYKLDAVEAAAVIGVKDR
                     YADEEIVAFVQLKEGMDLGEDEIRRHLRTVLANFKIPKQIHFKDGLPRNATGKVLKRV
                     LKEQFDGNK"
     gene            complement(4096..5289)
                     /locus_tag="WNA25_001522"
     CDS             complement(4096..5289)
                     /locus_tag="WNA25_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFA9824248.1"
                     /translation="MNPKHFIAFSALFAATQAEALPVASVSPDTVTVSPSAPYTDTNG
                     LLTDYGNAAASPWMKKLRSVAQGSGETFRILQIGDSHTAGDFFTDALRKRLQKTWGDG
                     GIGWVYPANVKGQRMAAVRHSGNWQSFTSRNNTGDFPLGGILAQTGSGGGMTLTASDG
                     KTGKQRVSLFAKPLLAEQTLTVNGNTVSANGGGWQVLDTGAALPLAIQTEMPWDIGFI
                     NIENPAGGITVSAMGINGAQLTQWSKWRADRMNDLAQTGADLVILSYGTNEAFNNNID
                     IADTEQKWLDTVRQIRDSLPAAGILIIGAPESLKNTLGVCGTRPVLLTEVQQMQRRVA
                     RQGQTMFWSWQNAMGGICSMKNWLNQGWAAKDGVHFSAQGYRRAAEMLADSLEELVRA
                     AAIRQ"
     gene            complement(5286..6269)
                     /locus_tag="WNA25_001523"
     CDS             complement(5286..6269)
                     /locus_tag="WNA25_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HFA9824249.1"
                     /translation="MKNFLSLFASILMSALIAVWFSQNPINAYWQQTYHRNSPLEPLA
                     AYGWWRSGAALQENAYALSDGIKTFLSGETPPTAQDGGSADMPPEAAASEAAPPAGGT
                     EWKQGTEAAAVRSGDKVFFAGDSLMQGVAPFVQKSLKQQYGIESANLSKQSTGLSYPS
                     FFDWPKTIEETLKKHPEISVLAVFLGPNDPWDFPVGKRYLKFASDEWAQEYLKRVDRI
                     LEAAHTHRVQVVWLGIPYMKKVKLDGQMRYLDKLLSEHLKGKIILIPTAQTLSGGKGR
                     YTDSVNVNGKPVRYRSKDGIHFTAEGQKLLAEKIMEKIVFEPSTQPSSTQP"
     gene            complement(6280..7716)
                     /locus_tag="WNA25_001524"
     CDS             complement(6280..7716)
                     /locus_tag="WNA25_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HFA9824250.1"
                     /translation="MPLLSVEFALFFIVFLPIYWGFAKYPSVQNLLLLAAGMGWLYHI
                     SPVFAAIIVLYSSCVYLLGELLRSDRESTRRFWLGCGIAASITVLGFFKYFDFFRPLI
                     AQYAGKGGAIDILMPLGLSYYTFQSVAYLVYCFRAPHAARFGWHELLLHLSFFPTVTS
                     GPIIRAAAFKSTDGEQAGALAQIRTRRPRSPVRPALAVSLILLGIAKKWWLAGMLAEN
                     WVSPVFENPAQFDGWGVLAGVYGYTFQLFLDFSGYSDLVIGMAMLLGFRLPKNFSAPL
                     RAANIRAFWDKWHISLSTWIRDYIYIPLGGSKKGFLRTQLNLMAAMVLSGIWHGYGWN
                     FLIWGALHGTALALLNTGDRYFGRDALCRLKYLAPLSWFVTFHFVCLSFVVFNTANPD
                     DAGAVFSALFANAGGWNAPQRADMLLLASFASLMLLYPYLQRAFDGTVKGLEKIPMWL
                     WFIQISIILLLIIVLAPSGIPGFIYANF"
     gene            complement(7846..9011)
                     /locus_tag="WNA25_001525"
                     /pseudo
     CDS             complement(7846..9011)
                     /locus_tag="WNA25_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713084.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
     gene            9452..9664
                     /locus_tag="WNA25_001526"
     CDS             9452..9664
                     /locus_tag="WNA25_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFA9824251.1"
                     /translation="METLLLDIVGMSCGGCVKSVTRILESVKGVASVEVSLENKSATV
                     GYDPAQTAAEALIEAVEDGGYDAALK"
     gene            complement(9643..9804)
                     /locus_tag="WNA25_001527"
     CDS             complement(9643..9804)
                     /locus_tag="WNA25_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824252.1"
                     /translation="MSEGTIARKFNQFILPVQLNQMPPEGAERASDGIGETQRLQTAF
                     LPLYFNAAS"
     gene            9864..11391
                     /locus_tag="WNA25_001528"
                     /pseudo
     CDS             9864..11391
                     /locus_tag="WNA25_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357698.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase regulatory domain-containing
                     protein"
     gene            11473..12570
                     /locus_tag="WNA25_001529"
     CDS             11473..12570
                     /locus_tag="WNA25_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFA9824253.1"
                     /translation="MNQTFTLPDTRPYPQNPIKNHLLLNAYQLAHNSSQASRKLSSGQ
                     LQTEIRGMLEQNHYINLSLALTMSPDAGTYAALLSSVNAVLDCEKEGEVQWFALPVVL
                     VSGCKKERAIEMKLPTEALFACLQNYPHLRALTQETQWLPYLVHSSDLSAVAPDEWWR
                     AKQNTEAAAQHLRRFAPRPLLLPEGQSVHVVYVLGFGSGKVQAALGQNLLQAGLPLMQ
                     VWQENLASEGITLFANPLSPDTPARALSDGSHTRQRMAMDVFAANAIRAVRMQGPRVG
                     VVAAAKAGGQILFGFNATDGAFEVVPQVFCWQLSFTDNIAVIQQNFLDLMAECRVEHV
                     YLLHNPLGEQENIPSYAEALKREGRNPFFSA"
     gene            12684..13798
                     /locus_tag="WNA25_001530"
                     /pseudo
     CDS             12684..13798
                     /locus_tag="WNA25_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246235.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
     gene            13923..14378
                     /locus_tag="WNA25_001531"
     CDS             13923..14378
                     /locus_tag="WNA25_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HFA9824254.1"
                     /translation="MKKPKILFVCLGNICRSPMAEYILRRRAAEAGIPLETDSAGTSG
                     WHDGEDMHRETAKILKKYGIDASGFTSRKIRQSDATAFDYIIAMDGKNLSELEKTFGR
                     RPEKIFKLTDLIPESGYDHVPDPWYMGDFEETYRLADAGCRALLEKISK"
     gene            14455..14931
                     /locus_tag="WNA25_001532"
     CDS             14455..14931
                     /locus_tag="WNA25_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HFA9824255.1"
                     /translation="MKTNFKQKIIEQARREDLQVTALREQVLDIVLQQSGVIKAYNVL
                     SQMQQQSEGVLAPPTAYRALDFWADQGVLHKVAAVNGYILCSHAQHECNDHCHDHEEA
                     EAHHSAFILVCTECGAADEQTLSHEWAALRAGVAESGFALKEEHVVLTGICKKCQK"
     gene            14919..15845
                     /locus_tag="WNA25_001533"
     CDS             14919..15845
                     /locus_tag="WNA25_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFA9824256.1"
                     /translation="MSEVKKTKVHLISGFLGTGKTTALKSLMAQKDPNEKWVIIVNEF
                     GEIGIDGAVLSDNGIPVAEIAGGCLCCTAGPQMGVTVQKMLRDAKPDRLMIEASGLAH
                     AASVIDELKAKPLDSLLEIGAVFTVVDPRQFINPDYAQQALYKDQIGICDVLAAGKTD
                     LCTPEQLAEFHDKAAKLFPPKAKVVEVQNAQLDIQWLDIPVVEKSRYRLKALPDNTMG
                     FQSQGFTFPAGRDFDGEKLTNFFNDLPKMTEGLVRAKGVFQVLGTWVWLNWVDGQWGA
                     NQVSWRRDSRFELIAKSFDADLIEQKLKDALE"
     gene            15910..16467
                     /locus_tag="WNA25_001534"
     CDS             15910..16467
                     /locus_tag="WNA25_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFA9824257.1"
                     /translation="MKPKFKTVLTALLLAVCLPSMAATRVLMETDMGNIRLVLDESKA
                     SKTVANFVRYARKGFYDNTIFHRVIGGFVIQGGGLTEDLVQKATDKAVANESGNGLKN
                     TVGTIAMARTADPDSAAAQFFINLADNGSLDYKNGQYGYTVFGRVESGMDTVSKIARV
                     KTATLGLYQNVPVQPVKIRRVVVGQ"
BASE COUNT         3882 a         4185 c         4674 g         3813 t
ORIGIN      
        1 cccggcagct aaaaatcctg cgggatcggt gtggaattta gggataatct agtacagccc
       61 catttcggcc ggtttgtgca tttcgtcatc cggaagccgg tattttgccg gcaggttttg
      121 actactttgc ccgatgaccg ggtttgtctg tttttaacaa aggagtgaga aggtttgtcc
      181 ctgtctgaat ttatagaacg ccgaacgtca tttaatccga tggttatttt gacgactttg
      241 ttttttgtgt gtgttttggt ggtattggtt ttaaccgtgc cggatcaggt gcagatgtgg
      301 ctcgaccggg caaaagaagt catttttacc gagttcagct ggttttatgt tttaacgttt
      361 tccatttttc tgggttttct gctgatactc tcggtcagcg gtttgggaaa catcaggcta
      421 ggacgggatg aagatgtgcc ggaattcggc ttcctgtcgt ggctggcgat gctgtttgcg
      481 gccgggatgg gcgtgggcct gatgtttttc ggcgtggcag agccgttgat gcattatttt
      541 tcggacatta cggtcggcgc gccggaacac aggcagcagc aggcattgct gcacacggtg
      601 ttccattggg gcgttcacgc ctggtcggtg tacggtacga ttgcattggc tttggcttat
      661 ttcggtttcc gctacaaact gccgcttgcc ctgcgttctt gtttttaccc cctgttgaaa
      721 gaaaaaattt ccggaaggtt cggcgatgcc attgatatta tggcgttgct tgctactttt
      781 ttcggcatca tcaccacatt ggggttcggg gcttcgcaac tgggcgccgg attgcaggaa
      841 atgggctgga ttgccgaaaa cagcttcggc gtgcaggtct tgattatcgc cgccgtaatg
      901 tccctcgccg tcgtttcggc aatatccggc gtggggaagg gcgtgaaggt gttgagcgag
      961 ttgaacctgg gccttgcgtt tttgctgctg ttttttgttt tggcggcggg ccccactgtt
     1021 tacctgttgt cggcattcgg cgacaacata gggaactacc tcggaaatct ggtgcgcctc
     1081 agtttgaaaa cttatgcgta cgaacgggaa cacaagccgt ggtttgaatc ttggacggtg
     1141 ctttattggg cgtggtggtg ttcttgggcg ccgtttgtgg gtttgtttat cgcgcgcatt
     1201 tcaaaggggc gcaccatccg cgagtttgtc ttcggggttt tgctcatccc cggcctgttc
     1261 ggcgttttgt ggtttaccgt cttcggcaat acggcgattt ggctgaatga cggggttgcg
     1321 gggggaatgc tcgaaaagat gacctcctct ccggaaacgc tgctttttaa attctttaat
     1381 tacctccccc tgcccgaact gacgagcatc gtcagcctgc tggtcatttc cctgtttttt
     1441 gtaacttctg ccgactccgg gatttatgtc ctgaacaata ttacctctcg ggacaaaggc
     1501 ttgagcgcgc cacggtggca ggcggttatg tggggcgtgc tgatgtctgc cgttgccgtt
     1561 ttgctgatgc gctcgggcgg actcggcaac ctgcagtcta tgaccctgat tgtttccctg
     1621 ccgtttgccc tgctgatgct gataatgtgt ttcagcctgt ggaaaggctt gagtgcggat
     1681 aagaaatatt ttgagacccg ggtcaaccct accagtgtat tttggacggg cggcaagtgg
     1741 aaagaacggc tggtgcggat aatgagccag acgcaggagc aggatatttt aaaattcctc
     1801 aaacataccg catcgcccgc tatgcacgag ttgcaacggg agctttcgga agaatacggc
     1861 ttgagcgtcc gggtcgataa gatgtttcat caggacgagc ccgcaatcga gttcgtcatt
     1921 cggaaagaga cgatgcgcga ttttatgtac gggattaagt ctgtcgggca ggatgtatcc
     1981 gaccagttga ttaacgacgg caagctgccg catatccggc accagacaac ttacaaaccc
     2041 tacgcttatt ttttcgacgg gcgcgtcggg tacgatgtgc agtatatgaa caaggacgag
     2101 ctgattgccg acattttgaa aaactacgaa cgttatttga tgttgttgga tgatgtcggt
     2161 caggaactga tggcgcacga gcaggtggaa ttggcagagt aagcgccttc cctccggcgt
     2221 ttgccaaaaa atgcggtgaa tcagattcac cgcatttttt tgccggacgg ggcttcggac
     2281 ggcacggcgt tcatttgttt ccgtcaaact gctccttcag cacccgtttc aatactttgc
     2341 ccgtagcgtt gcgcggcagc ccgtctttaa agtggatctg tttggggatt ttgaaatttg
     2401 ccagcacggt acgcaggtgg cggcggattt cgtcctcgcc caaatccata ccttccttca
     2461 attggacgaa ggcgacgatt tcctcgtcgg cataacggtc tttcacgccg atgacggcgg
     2521 cggcttcgac ggcatcgagt ttgtagattt cttcttcgat ctcgcgcgga tagacgtttt
     2581 gacctttgga aataatcaaa tcttttttgc ggtcgacgat aaagataaag ccgtcctcgt
     2641 ctatggtaac gaaatcgccc gttttcaacc agccgttgac gatggtttca tcggtggcgg
     2701 caggcatatt gaggtagccc cgcatcaccg aaccgcccct gacgatcagt tcgcccactt
     2761 cgccgcgcgg cacttcgacc aattcttcat cgacggcttt ggcttccaaa ccgggcaggg
     2821 ggatgccgac gctgcgggct ttttgccgtt cgggcgtatt gacggcgacg acgggcgagg
     2881 cttcgctcag tccgtagcct tccagcaatt tggcgcgggg gaacttggct ttaaaatcga
     2941 ggatggtttg ttccgccaaa ggcgcgccgc cgctgataaa caggcggatg cggttgaacc
     3001 atctgaaata ccaagggatt ttcgccttgc tcatcgcggt gtaaatcgcg ggtacgccca
     3061 aaaacacggt tgcgcgtttg agcagggcct gtttcaaaac gttggaaaag gggaaaacgg
     3121 atttgaccaa aataatcgaa cacgccatat aaatcggcag cagtaccata gccgtcagcg
     3181 tgaagctgtg gaacatcggc aggaaaacga taaagcggtc gcgtttggaa attttaaaga
     3241 tgcgttcgat gccgttcagg ttggcgaaca ggttggcgta actgattagc gcgcctttgg
     3301 gatgccccgt cgtgccggag gtgtagatga tgtgtgccaa atcatttatc cggggttggc
     3361 ggtccaagtc gggtttttcg gggaagcggc gcacgttttc aaaaaaggca tcgccttccg
     3421 ccgtttcgcc ggccggccgg cttttgtccg tccaaatgat tttttcgacg ggcgtttgcg
     3481 ccttcaggcc cgccaattct tttgacaggc cggccgaggc gaacaggaag cgcgccttgc
     3541 agtcattcag gatatacgcg tattcgctgt ttttcaaaaa tgtgttcatc ggtacggcga
     3601 ccgcgccgat ggcggagacg gcgaaatagg cggtaataaa ttctgtggaa ttggaaaccg
     3661 ccagcgcgac cgtgtcgccg aacttcacgc cgatattttg cagatacgcc gcgacggctt
     3721 cggcttcctg cttgagcgcg cggtaggcgg ttttttcctt gccgtcgaac actgccgtgc
     3781 cgttgccgtt tttgcggcag gcggcggcga gcatttcgta gaaattggca taagtccggt
     3841 tcatgaaact tcctttatgg ttgcggacgg cgaacgccct catttgaaaa caatatgccg
     3901 tctgaaaacg gaaggcggca gtttatcatc tttgccttca gacggcatat caaacgccgc
     3961 ctttgcgccg catgccggag gttcgccccc gtccttccta tgtctgacct gaatgccgtc
     4021 tgaatcaata tgaagatata cgtttcagac ggcatccccc tgttgtggaa ataccgtccg
     4081 cccccccctg tccgattatt gcctgattgc ggcggcgcgg acgagttctt cgaggctgtc
     4141 ggcaagcatt tccgccgcgc gccggtagcc ttgggcggag aagtgtacgc cgtctttggc
     4201 ggcccatcct tggttgagcc agtttttcat gctgcatatg ccgcccattg cgttttgcca
     4261 agaccaaaac atcgtctgcc cctgacgggc gacgcgccgc tgcatctgtt ggacttcggt
     4321 caggaggacg gggcgcgtgc cgcatacgcc gagcgtgttt ttcagggatt cgggcgcgcc
     4381 gatgatgagg atgccggcgg cgggcaggct gtcgcggatt tggcggacgg tatccagcca
     4441 tttttgttcg gtatcggcaa tgtcgatgtt gttgttgaag gcttcgttgg tgccgtagga
     4501 aaggataacc aaatcggcgc cggtttgggc aaggtcgttc atacggtcgg cacgccattt
     4561 cgaccactgg gtcaattgtg cgccgttgat gcccatcgcg gaaacggtaa tgccgccggc
     4621 gggattttcg atgttgatga agccgatgtc ccacggcatt tcggtctgta tggccagggg
     4681 cagtgccgcg cccgtatcca gtacctgcca gccgccgccg ttggcggaga cggtgttgcc
     4741 gttgacggtc agggtttgtt cggcgagcag cggtttggca aacagggaaa cgcgctgttt
     4801 gccggttttg ccgtcagacg cggtcagggt catgccgccg ccgctgccgg tttgggcgag
     4861 gatgccgccg agcgggaaat ctccggtatt gttcctgctg gtgaagcttt gccagttgcc
     4921 gctgtgacgg acggccgcca tgcgctgccc tttgacgttg gcggggtaaa cccagcctat
     4981 gccgccgtcg ccccatgttt tttgcaggcg tttgcgcagg gcgtcggtaa agaagtcgcc
     5041 ggcggtatgc gagtcgccga tttgcaggat gcggaaggtc tcgccgctgc cttgtgcgac
     5101 ggatcggagt tttttcatcc aaggcgaggc ggcggcgttg ccgtagtcgg tcagcagccc
     5161 gtttgtatcg gtgtaggggg cggacgggga aacggtaacg gtgtcggggc tgacggaggc
     5221 gacgggcagg gcttctgcct gcgtggcggc gaacagggcg gaaaatgcga tgaagtgttt
     5281 ggggttcatg gctgtgtact tgatggttgc gtactcggtt caaaaacgat tttttccatt
     5341 attttttccg ccagcagttt ttgtccttcg gcggtaaagt gtatgccgtc cttactgcgg
     5401 tagcggacgg gtttgccgtt gacgttgacg gaatcggtgt agcggccttt cccgccgctc
     5461 agtgtttgcg cggtgggaat caggatgatt ttgcctttca agtgttccga aagcagtttg
     5521 tcgaggtagc gcatctgacc gtcgagcttg acttttttca tgtaggggat gccgagccag
     5581 acgacttgga cgcggtgcgt gtgtgcggct tcaaggatgc ggtcgacgcg tttcaggtat
     5641 tcttgcgccc attcgtcgga agcgaatttg aggtagcgtt tgccgacggg gaaatcccac
     5701 gggtcgttcg ggccgaggaa gacggcgagt acgctgattt cgggatgttt tttcaaggtt
     5761 tcttcaatcg ttttcggcca gtcgaagaat gagggatagg aaagccccgt gctttgtttg
     5821 ctgaggttgg cggattcgat gccgtattgc tgtttcaggc ttttttgcac gaaaggcgca
     5881 acgccctgca tcagcgaatc tccggcgaaa aagactttgt cgccgctgcg gacggcggcg
     5941 gcttcggtgc cttgtttcca ttctgttccg ccggccggcg gggcggcttc ggatgcggcg
     6001 gcttcaggcg gcatatctgc cgaaccgccg tcttgagccg tcggcggcgt ttcgccggac
     6061 aggaaggttt tgatgccgtc tgaaagggcg taggcgtttt cttgcaacgc cgctccgctc
     6121 cgccaccatc cgtaggcggc aagcggttcg agcgggctgt tgcggtggta ggtctgctgc
     6181 cagtaggcgt tgatggggtt ttggctgaac cacacggcaa tcagggcaga catcagtatg
     6241 gaggcgaaaa gggaaagaaa gtttttcatg tccaaaccct taaaaattgg cataaataaa
     6301 gccgggtatc cccgaagggg cgaggacgat aatcagcagc aggatgatgg aaatctggat
     6361 aaaccacagc cacatcggga ttttttccaa acctttgacc gtgccgtcga aagcgcgttg
     6421 caggtaaggg tagagcagca tcaaggatgc aaacgaggca agcagcagca tatccgcccg
     6481 ttgcggcgcg ttccagccgc cggcattggc aaagagggca ctgaaaactg cgcctgcatc
     6541 gtcggggttt gcggtattga agacgacaaa gctaaggcag acgaaatgga aggtaacaaa
     6601 ccacgagagc ggcgcaaggt atttcagacg gcatagcgcg tcgcgtccga aataccggtc
     6661 gcccgtgttg agcagcgcca gtgccgtgcc gtgcagcgcg ccccaaatga ggaagttcca
     6721 gccgtagccg tgccagatgc ctgagagtac cattgccgcc atcaggttga gctgtgtccg
     6781 caaaaagcct tttttgctgc cgcccaaggg gatgtagatg tagtcgcgta tccaggtgga
     6841 aaggctgatg tgccatttgt cccaaaatgc gcggatattc gcggcacgaa gcggtgcgga
     6901 gaagtttttg ggcagccgga agcccagcag catcgccata ccgataacca aatcggaata
     6961 tccggaaaag tctaaaaaga gttggaaggt atagccgtac acgcctgcca acacgcccca
     7021 gccgtcgaat tgggcgggat tttcaaatac gggcgacacc cagttttccg ccagcatccc
     7081 cgccagccac cattttttgg caatgcccag taaaatcagg gaaacggcga gggcggggcg
     7141 gacgggcgaa cgcggtcggc gggtgcggat ttgcgccagt gcacccgcct gttcgccgtc
     7201 cgtgcttttg aatgcggcgg cgcggataat cgggccggag gtaacggtgg ggaaaaaact
     7261 caggtgcagc agcagctcgt gccacccaaa acgcgcggcg tgcggggcgc ggaagcagta
     7321 aaccagatag gcgacagact ggaaggtata ataggaaagc cccagcggca tcaggatgtc
     7381 gattgcgccg ccttttccgg catattgtgc aatcagcgga cggaaaaaat cgaaatattt
     7441 gaaaaagccc aaaacggtaa tcgaggcggc aatgccgcac cccagccaaa agcggcgcgt
     7501 actttcgcga tcggaacgga gcagttcgcc caaaaggtac acgcaggagg aataaaggac
     7561 gatgattgcc gcaaatacgg ggctaatatg gtagagccag cccataccgg cagccaaaag
     7621 cagcaggttt tggacggacg ggtatttcgc aaagccccag taaatcggca ggaagacgat
     7681 aaaaaacagt gcgaactcga cagacagcag cggcataaac catccttggg acaaaaaaat
     7741 aggcgttaaa acacggcaaa gcccgttatt ttaagcaggg gcggatattc cttcaatcaa
     7801 aatgccgtct gaagggcggg aggggcttca gacggcatcg ctcggtcagg ttcggatatg
     7861 gtggttcaaa ttcaggcaga cccgcaaacc tgccgccgcc gtcagcatac ccgcaacgcc
     7921 gatgcaggaa atgcccgagt attgcgtaac ctaatgtccc agcagtgcgc cgccgcctat
     7981 accgacgttg taaagctccg aataaatcga gttggcaagg tctgcggcat cggaggcgaa
     8041 atccaaaact ttggcaacca tccccaagct gacgaccaca atcgccgtcc cccatacgaa
     8101 caccaacgcg taaatggcgg caggaaaatg cgccaaaggc agcaaaagcc ccgaagagag
     8161 ggcgataagc gacaccgcgc ccgccaaaaa tgcgcgcgga tgtttggcaa accatttgcc
     8221 gaacaggtaa gacgcggcaa aacccgccag gccgtacagc cccggaacca ccgtaacctg
     8281 ccgcgcggag aaaccgccga tttggatgac gaagggctcg atgtagctgt atgcggtaaa
     8341 gtgcgccgta atcaccggca ccgttaccgc atacagcagc atcagttttt tccgtttcaa
     8401 aagcaggggc aggctgctta aagaaccggt attcacgctc ggcaggcgcg gcaggctttt
     8461 ggcaagcact gccatcaccg ccgccgaaca caggccgatc agcagaaaac tcgcctgcca
     8521 gcccaaatat tgtccgacca tacgcccgag cggaatcccc gccaccatcg ccatcaccgt
     8581 ccccgtgctg agcagcccca atgcctgatt cccttttccg acaagggcga tgcgtaccgc
     8641 cagcgaagcc gtaatggacc agaacaccgc gtgcgtcagc gcaatcccca cgcggcttgc
     8701 gagtaggatt tcaaaacgcc acgaggcaaa cgacaggatg tggctgacag taatacagcg
     8761 caaacaggac cagcagcagg ccgcgccgtt ccataattgc gcgtcagcag catcagcggc
     8821 agcggcagcg aagtcagcgc gacaatccac gcataaaccg taatcatcac acccgtttcg
     8881 gtcgccgcca tgccgaagct tcgtccgatg tcgctcaaaa gcgcgatggg gatgtattcg
     8941 accgtgttga aaataaatgc gccgattgcc aaggcaatga tgctcaacca aggtttgtgc
     9001 gaagcgttca tctcaattcc ttatatgtcg caccccgcca aaagggggcg cattatggtg
     9061 aattaacaaa aaccggtacg gcgttgcccc gccccggctc aaagggaacg attccctaag
     9121 gtgatgaagc accaggcgaa ccggttccgt accatttgta ctgcctgcgg cccgccgcct
     9181 tgtcctgatt tttgttaatt cactatactt ttcttgtctg ccggacggca aaatccaaat
     9241 cagggggaag cagccgcctc ccgttcggag gaacactcgg gacgctgctt tgaaaatcgt
     9301 tccatatgtt gaaacattgc cgcctcccga ttgggcaagg ttgccattat gtcgcaagca
     9361 tccgtgccgc accgtttccg cgtttgccca agttcacgct ccgacttaca ataacgcccg
     9421 tcagattgca gtaccaacga aaggaaaaat cgtggaaacc ctcctcctcg acatcgtcgg
     9481 catgagctgc ggcggctgcg tcaaaagcgt tacccggata ttagaaagcg taaaaggcgt
     9541 ggcaagcgtc gaagtcagcc ttgaaaacaa aagtgcgacc gtcggatacg accccgcgca
     9601 aaccgcagcg gaagcactaa tcgaagccgt agaagacggc ggctatgatg cggcgttgaa
     9661 ataaagcggc aaaaatgccg tctgaagcct ttgcgtttcc ccgatgccgt ctgaagcacg
     9721 ctccgcgcct tcgggcggca tttgatttag ttgaacgggc aggatgaatt ggttaaactt
     9781 gcgggcaata gtgccttcag acaaacaaaa tatgccgaac aaaaagaaaa aagccgcctt
     9841 tgcgcgctgc aacggagaac ggtgtgccgg acgtgtccga catcaaaaat tcatatgatg
     9901 atttgatgta cgaatccaaa gcattttccc aaaccgccat caacgccttg gaggcatgcg
     9961 cccgcatgat gggtttgaag cctacggcag ccgcaaaagc caaggtcttg gagctgggct
    10021 gttcgatggg cagaaacatc atcaacgcag gcactttatt acccggatgc ggaatttgtc
    10081 ggtatcgact tgtccggcag gcaggttgcg cagggcaacg ccatcattga aaaaatgggc
    10141 ttggaaaatg tgcgtctgga agaaaaagat attttgaccg tcgatgaatc attcgggaag
    10201 ttcgactata tcatcgtcca cggcatttgg tcgcgggtgc ccgacgcagt taaagacaaa
    10261 atcttttcga tttgccggaa caacctgacc gaatacggca ttgcctatat ttcatacaat
    10321 gtttaccccg gttggaagcg gcaggagcag ttgcgcgata ttatgcagtt tgccggcagg
    10381 gatgcgcttg gagaaccctt ggaagcaagg acgcggaaag gcttggatgc gatcaaggcg
    10441 ctggcggaaa ttttggaaaa cgacaagggc ttgggcggcg gcaaacttcc ggcgattcaa
    10501 aaaatattga atcacaatac ttattatgtc gcacacgaat atatggaaat attcaacaac
    10561 ccgatttacg ttaacggttt cattgaatgg gcaaaccgcc accggctggc gtatatcggg
    10621 gataccgatt tgcatgtgtc ttttgtttct tggatggcgg agcatacgcg ggagcggatt
    10681 ctggcattgg cagggggtga ttatattgcc aaagaatttt acagcgatat tttatccgac
    10741 cgccaattcc accgttcgct tttatgccgt gaggaagtcg gggatactgt caggcgtgat
    10801 gagtcggttg ccgtcgaagt gatagaaagt ttgaatttcc gcccggcaag aggggaaaca
    10861 atcaactttg atgaaaacga taccctgctt tcgggcatac gcgatgtgat gaaaaccgga
    10921 gaggcgttta aaacggaaga tgttgcggaa aatcttgccc gccgatttcc cggtctggaa
    10981 tttgaccgca tgaaaatcaa ttcccagctt ttattgcaaa ccattctcgg gcgtttttct
    11041 gtttcatcag acaatgcggg caaaccattt tttgaagacc ataaaaccta tgtgccggcg
    11101 cgctttacaa actatgccgc cgcctttgtg gaacacggtg cgggagcgtt tgtccggcct
    11161 gccaaccgtt acaacgaaag cacgccctca ttcggatacg ggcatttgta cattatgcgg
    11221 caattgtcgc ggccgacgag caaacaggca ttgattgaaa cggttgccga aaacttgaac
    11281 atcgtcagcg ccacgcccga cggtttgaca ttccatccgc ctgccgaagt gtatgtggaa
    11341 gaaatattgg cagacttggc agacaggcat tttctcgttt cggcggattg acgggcaagt
    11401 cccgacggca atccgtccga tatattttca gacaggaaag acacacactg cgaaaaacgg
    11461 tacacgacac atatgaacca gacatttacc ttgcccgata cgcgcccgta tccccaaaat
    11521 ccgattaaaa accacctgct gctcaatgcc taccagttgg cgcacaattc gtcacaggct
    11581 tcgcgcaaac tctcgtccgg gcagcttcaa accgaaatca gggggatgct tgagcaaaac
    11641 cactatatca acctttccct cgcgctgacg atgtcgcccg atgccggaac ttatgccgcg
    11701 ctgctttcca gtgtgaacgc ggtactcgat tgcgagaaag aaggcgaagt gcagtggttc
    11761 gccctgccgg tcgtgctggt gtccggctgc aagaaagaac gggcaatcga gatgaagctg
    11821 ccgacggagg cattgtttgc ctgcctgcaa aactatccgc acctgcgcgc gttgacgcaa
    11881 gagacacaat ggctgcctta tcttgtgcat tcttccgatt tgagtgcggt cgcgccggat
    11941 gagtggtggc gtgccaaaca aaataccgaa gcggcggcgc aacacttacg ccgtttcgcc
    12001 ccgcgccctt tgctgttgcc cgaagggcag tccgtccacg tcgtttacgt gctgggtttc
    12061 ggcagcggca aagttcaggc ggcattgggg cagaaccttc ttcaggcagg cttgccctta
    12121 atgcaggtat ggcaggaaaa tcttgcatcg gaaggcatta cgctgtttgc caatccgctt
    12181 tcccccgata ccccggcgcg cgcgctttca gacggcagcc acacgcgcca acgtatggcg
    12241 atggatgttt ttgcggcaaa cgcgatacgc gccgtccgga tgcagggtcc gcgcgtcggc
    12301 gtggtcgctg cggcaaaggc gggcggacag attctattcg gatttaatgc gaccgacggc
    12361 gcgtttgaag tcgtgccgca ggtgttttgc tggcagcttt cctttaccga taacattgcc
    12421 gtcatccagc agaatttcct cgacctgatg gcagaatgcc gtgtagaaca cgtttacctg
    12481 ctgcacaatc ccttgggcga acaggaaaac atcccgagct atgcggaagc gttgaaacgg
    12541 gaagggcgca atccgttttt cagcgcatag tgatttgtag tagtgatagg ctttctcatt
    12601 tctataataa atcaaaataa agagcacaac actttttcat tctgtgttgt gccttgagtg
    12661 aaacgaaagg atgaattata aatatgaaga ttgtaattgc accggattcg tttaaggaaa
    12721 gcttgacagc tcaacaggta gctgaagcaa taaaaagagg cttccaacaa tcgatagcag
    12781 atgtggaatg tctcctctgt cctgtcggtg atgggggaga aggcactgta gatgctatcc
    12841 gacattctct tgacctagaa gaaaaatgtc tccaagtgac aggacctttt ggacaaaaaa
    12901 agtcatgcgc tattttcaaa aagaacaact agccctattc gaagttgctg atttggttgg
    12961 tcttggaaaa atcccgctag agaaacgaaa tccattacaa atccaaactc gtggtattgg
    13021 agaattgatt cgccacctca ttagtcaaga aattaaagaa atctatattg gcgttggcgg
    13081 tacggccggt aatgatggag gtattgggat tgctgctggt ttaggttatc aattttatga
    13141 tgaggatgga aatgccttac ccgcttgcgg tcaatcctta ctaaacccag cttctgtttc
    13201 aatagaaaat cgctatgaaa ttcctgaaga tgttcacatt cgtattttag cagatgttgt
    13261 gagtccctta tgtggacacc aaggtgcgac ctatacgttt ggcaagcaaa aagggctgga
    13321 ttctactatg tttgaggccg tagatcaggc aatacaagat ttttatgaaa aagtcccccc
    13381 tgcaacatta aaacttaaag gagcaggagc tggtggaggc atcgctggtg gtttgtgtgc
    13441 ctttgctcag gcaagtatcg tatctggaat tgacacctgc ctggacttga ttgactttga
    13501 taagaaagtg tcagatgttg acttggttat cgttggtgaa ggaagactag atcgtcaaag
    13561 tttagcaggg aaagcgccta ttggtgtagc aaaaagaacc cctgtcggag ttcctgttgt
    13621 cgctatttgc ggcagtttcg ctgaagattt gccttcccta ccatttgaaa atatccaagc
    13681 tgccttttct attttggaga aaagtgaacc tttagaagat agtttgaaaa atgccagtct
    13741 ttatttggag catacggctt ctaatattgg acatttatta aatatgccta agatttagcc
    13801 aagccatttc ttccagatgg atgttttggc aggttcttcc ttatgcggtt acgattaacc
    13861 ggccgatgcc gtctgaaagg gtttccgctt cagacggcat cgcgttggaa cagggatggg
    13921 atatgaaaaa accgaaaatc ctttttgtct gcctcggcaa catctgccgt tcgccgatgg
    13981 cggaatacat tttgcgccgc cgtgccgccg aagcgggcat tcccctcgaa acggacagcg
    14041 cagggacatc gggctggcac gacggcgagg atatgcaccg tgaaacggca aagatattga
    14101 aaaaatacgg cattgacgct tcaggcttta ccagccgcaa aatccgccaa agcgatgcga
    14161 cggcgtttga ctacatcatc gctatggacg gcaagaattt gtccgaattg gaaaaaactt
    14221 ttggcaggcg gccggaaaaa atattcaagc tgaccgacct gatacccgaa agcggttacg
    14281 accatgtccc cgatccgtgg tacatgggtg attttgaaga aacatacagg cttgcggatg
    14341 caggctgtcg ggcattattg gaaaagattt ccaaataaag caattgaata caaacacaaa
    14401 tataaaaccg cctcctgtcg tatcggtcgt tcggacggca taaacaggga atttatgaaa
    14461 acaaatttca aacagaaaat tatcgaacag gcacgcagag aggacttgca ggtaaccgct
    14521 ttgcgcgagc aggtattaga tattgtcttg cagcaaagcg gcgtgattaa agcctacaat
    14581 gttttgtcac agatgcagca gcagagcgaa ggcgtgcttg caccgcctac cgcctaccgt
    14641 gcccttgatt tttgggcgga tcagggcgtt ttacacaaag tggcggcggt caacggctat
    14701 attttgtgca gccacgcgca gcacgagtgc aacgaccatt gccacgacca cgaagaagcc
    14761 gaagcgcacc acagcgcgtt tattttggtc tgcaccgaat gcggcgcggc ggacgagcaa
    14821 accctcagcc acgagtgggc ggcactgcgt gcaggcgttg ccgaaagcgg ttttgcgctg
    14881 aaagaagaac acgttgtttt aactggaatc tgtaaaaaat gtcagaagtg aaaaaaacca
    14941 aagtccacct gatttcaggt tttctgggaa caggcaaaac caccgcgctc aaaagcctga
    15001 tggcgcaaaa agacccgaac gaaaaatggg tcatcatcgt caacgagttc ggcgaaatcg
    15061 gcattgacgg cgcggtattg agcgacaacg gcatccctgt ggcagaaatc gccggcggct
    15121 gtttatgctg caccgccggc ccgcaaatgg gcgtaaccgt gcagaaaatg ctgcgcgacg
    15181 ccaaacccga ccgcctgatg attgaggcaa gcggactggc gcacgccgcc agcgtcatcg
    15241 acgaattgaa agccaaacct ctggacagtc ttttggaaat cggcgccgtc tttaccgtcg
    15301 tcgatccgcg ccagttcatc aatcccgatt acgcgcaaca ggcgttgtat aaagaccaaa
    15361 tcggcatttg cgacgtattg gccgccggca aaaccgattt atgcaccccc gaacagcttg
    15421 ccgaatttca tgacaaagcg gcaaaactgt tcccacccaa agccaaagtg gtcgaagtcc
    15481 aaaacgcaca actcgatatc caatggctcg atattcccgt agttgaaaaa tcacgctacc
    15541 gcctcaaagc cctgccggac aacaccatgg gcttccagtc gcaaggtttc acattccccg
    15601 ccggacgcga tttcgacggc gaaaaattga ccaacttctt caatgatttg cccaaaatga
    15661 ccgaaggact cgtccgcgcc aaaggcgtgt tccaagtatt gggaacgtgg gtgtggctca
    15721 actgggtgga cgggcagtgg ggcgcgaacc aagtgtcttg gcggcgcgat tcgcgtttcg
    15781 agctgattgc caaatcgttt gacgcggatt taatcgaaca aaaacttaaa gacgcattgg
    15841 aatagcgttc acgcgctttc agacggcata tgaaatgaaa atgccgtctg aaggctttag
    15901 gaggacggga tgaaacccaa attcaaaacc gttttaaccg cgctgctttt ggcggtttgc
    15961 ctgccgtcta tggcggcaac ccgcgtcctg atggaaaccg atatgggcaa tatccgtttg
    16021 gttttggacg aatccaaagc ctccaaaacc gttgccaatt tcgtgcgcta tgcccgaaaa
    16081 ggcttttacg acaacacgat tttccaccgc gtcatcggcg gcttcgtcat ccaaggcggc
    16141 ggattgaccg aggacttggt gcaaaaggca accgataagg ccgttgccaa cgaatccggc
    16201 aacggcttga aaaacaccgt cggcactatc gcaatggcgc ggacggcaga ccccgattcc
    16261 gccgccgccc aattctttat caatctggcg gacaacggct cgctcgacta caaaaacgga
    16321 caatacggct acaccgtttt cggcagggtc gaaagcggaa tggacaccgt ttccaaaatc
    16381 gcccgcgtca aaaccgccac gctcggcctt tatcaaaacg tacccgtaca gcccgtcaaa
    16441 atccgtcgcg ttgttgtcgg gcagtaacac gcagacagac gttcagacgg cgtcgcccgt
    16501 ttcccaaaaa acgccgttta aagtaaaaaa atattttaaa acagacagtt gata
//
LOCUS       DAWTQS010000050        16010 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.050, whole genome shotgun sequence.
ACCESSION   DAWTQS010000050 DAWTQS010000000
VERSION     DAWTQS010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16010
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.050"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            344..2728
                     /gene="ppsA"
                     /locus_tag="WNA25_001535"
     CDS             344..2728
                     /gene="ppsA"
                     /locus_tag="WNA25_001535"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFA9824258.1"
                     /translation="MADNYVIWFENLRMTDVERVGGKNASLGEMISQLTEKGVRVPGG
                     FATTADAYRAFLAHNGLNERISAALAKLDVEDVSELARVGKEIRQWILDTPFPEQLDA
                     EIEAAWDKMVTDADGADISVAVRSSATAEDLPDASFAGQQETFLNINGLDNVKKAMRH
                     VFASLYNDRAISYRVHKGFEHDIVALSAGVQRMVRSDSGASGVMFTLDTESGYNQVVF
                     VTSSYGLGENVVQGAVNPDEFYVFKPTLKAGKPAILRKTMGSKHIKMIFTDKAEAGKS
                     VTNVEVPEEDRNRFSITDEEITELAHYALTIEKHYGRPMDIEWGRDGLDGKLYILQAR
                     PETVKSQEEGSRNLRRYAINGEKTVLCEGRAIGQKVGQGKVRLIKDASEMDSVEAGDV
                     LVTDMTDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGNATELLENGQE
                     VTVSCAEGDTGFIYAGLLDVQITDVALDNMPKAPVKVMMNVGNPELAFSFANLPSEGI
                     GLARMEFIINRQIGIHPKALLEFDKQDDELKAEIIRRIAGYASPVDFYVDKIAEGVAT
                     LAASVYPRKTIVRMSDFKSNEYANLVGGSVYEPHEENPMLGFRGAARYVAESFKDCFA
                     LECKALKRVRDEMGLTNVEIMIPFVRTLGEAEAVVKALKENGLERGKNGLRLIMMCEL
                     PSNAVLAEQFLQYFDGFSIGSNDMTQLTLGLDRDSGLVSESFDERNPAVKVMLHLAIS
                     ACRKQNKYVGICGQGPSDHPDFAKWLVGEGIESVSLNPDTVIETWLYLANELNK"
     gene            2949..4208
                     /gene="rho"
                     /locus_tag="WNA25_001536"
     CDS             2949..4208
                     /gene="rho"
                     /locus_tag="WNA25_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFA9824259.1"
                     /translation="MHVSELQTLHISKLLELAEEHGIENANRFRKQDLVFAIVRQMMK
                     KGEGFTCSGTLEILPDGFGFLRSADTSYLAGPDDIYVSPTQIRRFNLHTGDTIEGSVR
                     VPKDNERYFALVRLDSINGDHPEVCRHKILFENLTPLFPTEQLKLERDLKSEENLTGR
                     AIDLISPIGKGQRALLVAPPKIGKTVMLQNIAHAVTANYPEVELIVLLIDERPEEVTE
                     MSRSVRGEVVSSTFDEPAQRHVQVAEMVLEKAKRMVEHKKDVVILLDSITRLARAYNT
                     VVPASGKILTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALVETGSRMDDVIYEE
                     FKGTGNMELHLDRRMAEKRLFPAININKSGTRREELLVPNDQLQRMWLLRKFLHPMDE
                     IEATEFLNGKIKASKNNDDFFELMRGK"
     gene            4826..6130
                     /locus_tag="WNA25_001537"
     CDS             4826..6130
                     /locus_tag="WNA25_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFA9824260.1"
                     /translation="MKKHIWAASLLPASLSAEPLNWWKPYSAVNSGDTAWVMTAAALV
                     LLMTLPGLALFYGGMVRKKNLLSTMMHSFSIATLVGILWVAVGYSLAFTPGNAFIGGL
                     GRVFLSGMQIDAAARMLTVSPNAPTVPEPVFMFFQMTFAIISTAIITGAFAERMKYSA
                     MMLFSGIWFLLVYVPGAHWVWGGGFMSKGGVLDYAGGTVVHINAGIAGLVAALVLGRR
                     IGYGREAMPPHNMAMTLIGAAMLWFGWFGFNAGSALAADAAAGMAMAVTQVSAVFGAA
                     GWLACEKIAGHKPSALGLASGAVSGLVGITPAAGFTGPSGAAAIGILTAAACFVSVTV
                     VKHKLRYDDSLDAFGIHGFGGLVGGILTGIFFDNRIFGGDAAVWQQLWIQVKDGFIMA
                     AYSGLMSWAILKAVGKICGGLRVGKDVEREGLDLNIHGEHVE"
     gene            complement(6224..7519)
                     /locus_tag="WNA25_001538"
     CDS             complement(6224..7519)
                     /locus_tag="WNA25_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFA9824261.1"
                     /translation="MIRPDFQEYLPSYYFSSVNPHTVYPKLQCRLKAETCIIGGGLSG
                     LCTALPLAEHGHEAVVLEAARIGFGASGRSGGQVISDYACGMGEIEKQVGLEQAQWFW
                     QQSLQAVELVDERVRKHAIDCDWQRGYATVAVRPQHWEELQQWHEHAQRHYGASHYQL
                     WDKAELKQQLDSDMYQGAQFDPLSGHLHPLNYTLGVASAAAEAGAQIFEQSPMTRIEP
                     YQNGWLVYTPEGSVECKNVVYAVNTYVGLNPIFRPLERKAIAVSTFIIATEPLGARTK
                     GLIRNNMAVCDNRHILDYYRLSADGRLLFGGKDNEFIDNPARMTELVRQDMLKVFPQL
                     ADVRIEYSWGGECDITANLVPHFGRLTSNVFYTQGYSGHGMAITGIAGLAVAEAILGD
                     ECRLKPFEQLRQPNIILQPFLRKLGSFLGSKYYQWKDSR"
     gene            complement(7646..8533)
                     /locus_tag="WNA25_001539"
     CDS             complement(7646..8533)
                     /locus_tag="WNA25_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFA9824262.1"
                     /translation="MQKSKLSWFLKLMLALSLAFLYIPLVVLVIYSFNESKLVTVWGG
                     FSTKWYGALLENDTILEAAWLSLRIAVVSSLAAVVLGTLAGYAMARIKRFRGSTLFAG
                     MISAPMVMPDVITGLSMLLLIIQVQIFLQGSEWLQHLYFDRGFFTIFLGHTTLCMAYI
                     TVVIRSRLVELDQSLEEAAMDLGARPLKIFFVITLPLIAPAIASGFLLGITLSLDDLV
                     ITSFLSGPGSSTLPQVIFSKIKLGLDPQMNVLATILIGIIGTLVIIVNYWMMRQATKR
                     NREAAEAYRQEKLAAEKAN"
     gene            complement(8533..9498)
                     /locus_tag="WNA25_001540"
     CDS             complement(8533..9498)
                     /locus_tag="WNA25_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFA9824263.1"
                     /translation="MNLNKLKNKLFRRPGQRAVIAVPYIWLLVLFLIPFAIVLKISSA
                     EQEIAIPPFTPLTTIDEDLGRLNIAVSYQNYADIFQNFWHTLNPFGDSENSNIYLMTY
                     WSSIKTALTTTVICLLVGYPTAYAISRANPSVRNGLLLAIMLPFWTSFLLRVYAWMGL
                     LGHNGIINNLLIKMGIIREPLDLFYNAFSLNLVMVYAYLPFMILPLYTQLVKLDSRLL
                     EAASDLGAGPVKSFLTITLPLSKTGIIAGSMLVFVPAVGEFVIPELVGGSENLMIGKV
                     LWQAFFDQNNWPLASAVAVVMVALLVVPIALFQHYENRELEEGAK"
     gene            complement(9514..10638)
                     /gene="potA"
                     /locus_tag="WNA25_001541"
     CDS             complement(9514..10638)
                     /gene="potA"
                     /locus_tag="WNA25_001541"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HFA9824264.1"
                     /translation="MTATTASSAKPYLQIQGLVKKFGDNYAVDNIDLNIYQNEIFALL
                     GSSGSGKSTLLRMLAGMESPNQGKIILDGQDITKLAPYDRPINMMFQSYALFPHMTVE
                     QNIAFGLKQDKMPKGEIDARVEEMLRLVQMTKFAKRKPHQLSGGQQQRIALARSLAKR
                     PKILLLDEPLGALDKKLRQQTQLELVNTLEQVGVTCIMVTHDQEEAMTMATRIAIMSD
                     GQLQQVGTPSDVYDYPNSRFTAEFIGETNIFDGVVIENHADYAVIECEGLENHVRIDH
                     GLGGPSEQDLWVSIRPEDIDLYKEKPEYLGDYNWAKGTVKEIAYLGSFAIYHIKLANG
                     RVVKSQVPAPYWYVRNITPPTWDETVYISWPENQPTPLFR"
     gene            complement(10916..11185)
                     /gene="rpsO"
                     /locus_tag="WNA25_001542"
     CDS             complement(10916..11185)
                     /gene="rpsO"
                     /locus_tag="WNA25_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFA9824265.1"
                     /translation="MALTVEQKAQIVKDFQRKEGDTGSSEVQVALLTFRINDLTPHFK
                     ANPKDHHSRRGLLKMVSQRRRLLAYLRRTQPDTYRALITRLGLRK"
     gene            complement(11390..12325)
                     /gene="secF"
                     /locus_tag="WNA25_001543"
     CDS             complement(11390..12325)
                     /gene="secF"
                     /locus_tag="WNA25_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFA9824266.1"
                     /translation="MELFKIKRDIPFMSYGKLTTFISLVTFIAAVFFLVARGLNFSVE
                     FTGGTVMEVQYQQGADVNKMRERLDTLKMGDVQVQALGTNKHIMIRLPNKEGVTSAQL
                     SNQVMDLLKKDSPDVTLRQVEFIGPQVGEELVNNGLMALGFVVIGIIIYLSMRFEWRF
                     AVSAIIANMHDIVIILGCFAFFQWEFSLTVLAGILAVLGYSVNESVVVFDRIRENFRK
                     PAMRGHTVPEVIDNAITATMSRTIITHGSTEAMVVSMLVFGGAALHGFSMALTIGIVF
                     GIYSSVLVASPLLLMFGLSRDNIAKEAKQKEEIVV"
     gene            complement(12329..14182)
                     /gene="secD"
                     /locus_tag="WNA25_001544"
     CDS             complement(12329..14182)
                     /gene="secD"
                     /locus_tag="WNA25_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFA9824267.1"
                     /translation="MNRYPLWKYLLIVFTIAVAAVYSLPNLFGETPAVQVSTNRQAII
                     INEQTQSKVDAALKNAGIQTDGMFVVDNSLKVRFKDTETQLKARDVIENTLGEGYITA
                     LNLLADSPEWMAKIKANPMFLGLDLRGGVHFTMQVDMKAAMQKTFERYSGDIRRELRR
                     EKIRSGTVRQAENSLTVPLQDAGDVQKALPQLLKLFPEATLNSEGSNIVLALSEEAVN
                     KVRSDAVKQNITTLHNRVNELGVAEPIIQQSGLDRIVVQLPGVQDTAKAKDIIGRTAT
                     LEVRMVEDDPAKLREALEGNVPSGYELLSSGGEHPETLLISKQVELTGDNINDAQPSF
                     DQMGAPAVSLSLDSAGGSIFGELTAANVGKRMAMVLIDQGKSEVVTAPVIRTAITGGR
                     VEISGSMTTAEANDTSLLLRAGSLAAPMQIVEERTIGPSLGKENIEKGFHSTLWGFAI
                     VAAFMVVYYRLMGFFSTIALSANILFLIGILSAMQATLTLPGMAALALTLGMAIDSNV
                     LINERIREELRAGVPPQQAINLGFQHAWATIVDSNLTSLIAGIALLVFGSGPVRGFAV
                     VHCLGILTSMYSSVVVFRALVNLWYGRRRKLQNISIGAVWKPEAETAAGKE"
     gene            complement(14394..14660)
                     /gene="yajC"
                     /locus_tag="WNA25_001545"
     CDS             complement(14394..14660)
                     /gene="yajC"
                     /locus_tag="WNA25_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFA9824268.1"
                     /translation="MNQAVAQFAPLVLIMVVFYFLIMRPQQKKFKAHQAMLAALKAGD
                     KVVLAAGFKGKVTRVGEQFFTVDIGQGTKIEVEVERNAIAAKVD"
     gene            14791..15909
                     /locus_tag="WNA25_001546"
     CDS             14791..15909
                     /locus_tag="WNA25_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HFA9824269.1"
                     /translation="MPLTRLFLKLYALLRLFLGKNAQTAWIFHPACTGHDPGANHPDS
                     PDRILCIEQALRRAGIWQHLQTVEAEEISDTRLALVRSSKYLNRLESCLPEDGKICRL
                     DDDTVISKNSLSAARFSAGSAVQAVDMVMKRKAWHAFCAARPPGHHAKSGKAGGFCLL
                     NNVAAGVMHAIAEYRLKRIAVIDFDVHYGDGTAEIFKDDPRILFFNLFETDLFPFPEN
                     NGMPDGGNMVHLPLPSGTGSRTFREAVRRQWLPRLAAFKPELVLLSAGFDAHRLDESG
                     RLNLHEADFAWLTHKIIQTASSCPGKIVSVLEGGYTLEPLAQSAAEHIRVLAGLGKSD
                     AATAYQKTLNPTKKRFAKPKTGQVRQPTQSDRYDINAV"
BASE COUNT         4031 a         4370 c         4003 g         3606 t
ORIGIN      
        1 aacatatctg ctaatctggt acagacccaa aaacaaatca tattttaaaa caaattatct
       61 caaaatcaga tttctttaaa ataaaattcc aaaaaaaaga aacaggaaaa atacggcata
      121 aaacattaac ctattcaaaa ccatctgttt tacaaaggaa atacccgaaa aaaacaatat
      181 gcctgccatc ggtttcaaac cgctacacag ccggaacccg ctatttttca gacggcgcag
      241 cggttgtccg gacagataaa aacgtgacag cggcggaacg aatcgcttaa aataagcaag
      301 cggatttgtt tcttttttaa catattttgg attggacaca caaatggccg acaactacgt
      361 aatctggttt gaaaacctgc gtatgacaga tgttgaacgc gtgggcggta aaaacgcctc
      421 gctgggcgaa atgatcagtc aactgaccga aaaaggcgtt cgcgtccccg gtggctttgc
      481 caccacagcc gatgcctacc gcgcatttct cgcacacaac ggtctgaacg aacgcatttc
      541 cgccgctctg gcaaaattgg atgtcgaaga cgtttccgaa ctggcacgcg tcggcaaaga
      601 aatccgccaa tggattttgg acacgccctt ccccgaacag ctcgatgccg aaatcgaagc
      661 ggcatgggac aaaatggtta ccgatgccga cggcgcggac atttccgttg ccgtacgctc
      721 ttccgcaacc gccgaagacc tgcccgacgc atcatttgcc ggccaacagg aaaccttcct
      781 gaatatcaat ggcttggata acgttaaaaa agcgatgcgc cacgttttcg cctccctgta
      841 caacgaccgc gccatttcct accgcgtcca caaaggcttc gaacacgaca tcgtcgccct
      901 ttccgccggc gttcaacgca tggtgcgttc cgacagcggc gcatcaggtg tgatgttcac
      961 cctcgacacc gaatccggct acaaccaagt cgtctttgtt acctcctctt acggtctggg
     1021 cgaaaacgtc gtacaaggcg cggtcaaccc ggacgaattt tatgtgttca aacccacgct
     1081 caaagcaggc aaacccgcca tcctgcgcaa gaccatgggt tcgaaacaca tcaaaatgat
     1141 tttcaccgac aaagccgaag ccggcaaatc ggtaaccaac gtcgaagtcc ccgaagaaga
     1201 ccgcaaccgc ttctccatta ccgacgaaga aatcaccgag ttggcacact acgcgctgac
     1261 catcgaaaaa cactacggcc gcccgatgga tatcgaatgg ggacgcgacg gcctggacgg
     1321 caaactctac atcctgcaag cccgccccga aaccgtcaaa tcccaagaag aaggcagccg
     1381 caacctgcgc cgctacgcca tcaacggcga aaaaaccgtc ttgtgcgaag gtcgtgccat
     1441 cggtcaaaaa gtcggtcagg gcaaggtgcg cctgattaaa gatgcttccg agatggattc
     1501 cgtcgaagcc ggcgacgtac tggttaccga catgaccgat ccggattggg aacccgtgat
     1561 gaaacgtgct tccgccatcg tgaccaaccg cggcggacgc acctgccacg ccgccatcat
     1621 cgcgcgcgaa ctgggtattc ctgccgtcgt cggctgcggc aatgcgaccg aactcttgga
     1681 aaacggtcag gaagttaccg tatcctgtgc cgaaggcgat accggcttta tctatgccgg
     1741 cctgctggac gtacaaatta ccgatgtcgc cttggacaat atgccaaaag cacccgtcaa
     1801 agtcatgatg aacgtcggca atcccgaact cgcattcagc ttcgccaatc tgcccagcga
     1861 aggcatcggc ttggcgcgta tggaatttat catcaaccgc caaatcggca tccacccgaa
     1921 agccttgttg gaatttgaca aacaagacga cgaactgaaa gcggaaatta tccgccgcat
     1981 cgccggttac gcatcccctg tcgacttcta cgtcgataaa atcgccgaag gcgtggcaac
     2041 gcttgccgca tcggtttatc cgcgtaaaac catcgtccgt atgtccgact tcaaatccaa
     2101 cgaatacgcc aacctggtcg gcggcagcgt atacgaaccg catgaagaaa acccgatgtt
     2161 gggcttccgt ggtgcggcgc gttatgttgc cgagagcttc aaagactgtt tcgccttgga
     2221 atgcaaagcc ttgaaacgcg ttcgtgacga aatgggtctg accaacgtcg aaatcatgat
     2281 tccgttcgtc cgcactttgg gcgaagccga agcagtggtt aaggcattga aagaaaacgg
     2341 cttggaacgc ggcaaaaacg gcctgcgcct gattatgatg tgcgagctgc cgagcaacgc
     2401 ggtattggcg gaacaattcc tgcaatactt cgacggcttc tccatcggct cgaacgacat
     2461 gacccagctg accctcggcc tcgaccgaga cagcggcttg gtgtccgaat cgtttgacga
     2521 acgcaaccct gccgtcaaag tgatgctgca tcttgccatc tccgcctgcc gcaagcagaa
     2581 caaatatgtc ggtatctgcg gtcaaggtcc gtccgaccat ccggactttg ccaaatggct
     2641 ggttggagaa ggcatcgaaa gcgtgtctct gaacccggat accgtcatcg aaacttggct
     2701 gtatttggcg aatgaattga acaaataatc aattcccata cccccaagcc tgaaaagcgc
     2761 gggggtattt tttccaagca gctccgttca gacggcattt cctgtcgatg ccccgtccgc
     2821 gataatattt gacacccacg cgccgactgc ctacaattcc cccctctccg agcaaccggc
     2881 aacagtcagc ttcttctttc agacggcatc cacccgtctt ttcctttcta ttctatatat
     2941 cattgattat gcacgtctcc gaattacaaa ccctgcacat ttccaaactc ttagaattgg
     3001 cggaagaaca cggcatcgaa aacgccaacc gattccgcaa acaagacctc gtatttgcca
     3061 tcgtccgcca gatgatgaaa aaaggcgagg gttttacctg ctccggcacg ctcgaaatcc
     3121 tgcccgacgg cttcggcttc ctccgcagcg cggacacgtc ctatcttgcc ggccccgacg
     3181 acatctatgt ctcgcccact caaatccgcc gcttcaacct gcatacgggc gacaccatcg
     3241 aaggcagcgt gcgcgtcccc aaagacaacg aacgctattt tgccttggtc agactggatt
     3301 ccatcaatgg cgaccacccg gaagtatgcc gccataaaat cctgtttgaa aacctgaccc
     3361 cgctgtttcc gaccgaacag ttgaagctgg aacgcgactt aaagtccgaa gaaaacctga
     3421 ccggacgcgc catcgacctg atttcgccca tcggcaaagg tcagcgcgcc ctcttggttg
     3481 ccccgcccaa aatcggtaaa accgtgatgc tgcaaaacat tgcccacgcc gttaccgcaa
     3541 actatcctga agtcgaactc atcgtcctct tgattgacga acgccccgaa gaagtaaccg
     3601 aaatgagccg ttccgtccga ggtgaagtag tctcctccac ctttgacgaa ccggcgcaac
     3661 gccacgtcca agttgccgaa atggtgcttg aaaaagccaa gcgtatggtg gaacacaaaa
     3721 aagacgtggt catcctgctg gattcgatta cccgccttgc ccgcgcctac aataccgtcg
     3781 tccccgcctc gggcaaaatc ctgaccggcg gtgtcgatgc caacgcgctg caccgcccca
     3841 aacgtttctt cggtgcggcg cgcaacgtgg aagaaggcgg ttcgctgacc attatcgcca
     3901 ccgcattggt tgaaaccggc agccgtatgg acgatgtgat ttacgaagaa ttcaaaggta
     3961 ccggcaatat ggaattgcac ctcgaccgcc gtatggcgga aaaacgcctc ttccccgcca
     4021 tcaacatcaa caaatccggc acgcgccgcg aagagctgct tgtgccgaac gaccagttgc
     4081 aacgtatgtg gttactgcgc aagttcctgc acccgatgga cgaaatcgaa gctaccgagt
     4141 tcttaaacgg aaaaatcaaa gcatctaaaa ataatgatga tttctttgaa ctgatgcgcg
     4201 gaaaataaac gcgccgcccg cattaatgcg aaatgccgtc tgaagcctga aaatcgggtt
     4261 tcagacgaca ttttcattcg cacggccggc gcagctcccc ccccccccgc taacggcgca
     4321 accgccggca gtgtccgtgt ccgcctgccg aaggcgcggc ctttgcaaaa ccggctcgaa
     4381 cgcatcccta ccggcaatga aaaccgatgc gggaacttga ttcaaggttg cgccgaaccg
     4441 cgcccatttt tgtataaatt ttatttttta tttccaatta gaaattttta atatattaat
     4501 caataaatta attttataaa ataaaaatat tgtcaacaat cttttgactt atcgcccaaa
     4561 cctctgtata ttttcctaca gtaaattgtt gacaatccat acgcccgcat atgcgccgcc
     4621 taaggataaa tcctcccgcc ggacaacggg ggcaagggat aggatgcgat atttccatat
     4681 tcaaacaagg gatttgtttc acgcacaggg cggcacatcg gcaaaatccc cgcgccggtc
     4741 cggcagggct tgcgcccatc ccggacaagc cccgaccccg cctttccgaa agacgggccc
     4801 aaccattaag gaaactttaa tcaaaatgaa aaaacacata tgggcggcat ctttgctgcc
     4861 ggcatcccta tcggcagaac ctttaaactg gtggaaacct tattccgccg tcaattcggg
     4921 cgataccgcc tgggtgatga ctgcggctgc cttggtactg ttgatgacgc ttcccgggct
     4981 ggctttattc tacggcggta tggtgcggaa aaaaaacctg ctctcgacga tgatgcacag
     5041 cttttccatc gcgacattgg tgggcatcct ttgggtcgcc gtcggctatt ctttggcgtt
     5101 cacgccggga aatgccttta tcggcggttt ggggcgcgta tttttaagcg ggatgcagat
     5161 agacgctgcc gcacggatgc tgaccgtgtc gcccaatgcg ccgactgttc ccgaaccggt
     5221 atttatgttt tttcagatga cgtttgccat tatttcgact gccattatta ccggcgcgtt
     5281 tgccgaacga atgaaatatt cggcaatgat gctgttttcg ggcatatggt ttttattggt
     5341 ttatgtgccg ggcgcgcatt gggtgtgggg cggcggcttt atgagcaagg gcggcgtatt
     5401 ggattatgcc ggcggtacgg tggtgcacat caatgccggt atcgcgggac tcgtcgccgc
     5461 cttggttttg ggcaggcgca taggctacgg gcgcgaggcg atgcctccgc acaatatggc
     5521 gatgacgctg attggcgcgg caatgttgtg gttcggctgg ttcggcttta acgctggttc
     5581 ggcgcttgcg gcagacgcgg cggcggggat ggcgatggcg gtaacgcagg tgtcggccgt
     5641 attcggcgcg gcaggctggc ttgcctgcga gaaaatagcg ggacacaaac cttctgcttt
     5701 ggggctggct tccggcgcag tttccgggct ggtcggcatc acccctgccg ccggctttac
     5761 cggtccgtcg ggcgcggccg ccatcggtat attgactgcc gccgcgtgct ttgtgtccgt
     5821 caccgtcgtc aaacacaaat tgcgttacga tgattctttg gacgctttcg gcatacacgg
     5881 attcggcggg ctggtaggcg gaatattgac cggcatcttt ttcgacaacc gcattttcgg
     5941 tggggatgcg gcagtttggc agcagttgtg gatacaggta aaagacgggt tcattatggc
     6001 ggcatacagc gggctgatga gttgggcgat tttgaaggct gtggggaaaa tctgcggcgg
     6061 tctgcgcgtc ggcaaggatg tcgaacgcga aggtttggat ctgaatatcc acggcgaaca
     6121 cgtggaataa ggacggctat gccgtctgaa gcctgaaaat cgggtttcag acggcatttt
     6181 tcacgtttgc cgccgatggg gataaacata ctgcctgcga cgcttaacgg ctgtctttcc
     6241 actgataata tttcgagccg aggaaagaac cgagtttgcg caaaaacggt tgcaggataa
     6301 tattcggctg gcgcaactgt tcaaacggtt tcagacggca ttcgtcccct aaaattgctt
     6361 cggcaaccgc caggcctgca atgcccgtta tcgccatccc gtgcccggaa taaccttgcg
     6421 tataaaaaac attcgaggtc aaacgtccga aatgcgggac aaggttggcg gtaatatcgc
     6481 actccccgcc ccacgaatat tcgattctaa catcggcaag ctgcggaaaa actttaagca
     6541 tatcttggcg gacaagctcg gtcatacgcg caggattgtc gataaattcg ttatccttac
     6601 cgccgaaaag cagcctgccg tccgcgctga ggcggtaata atccaaaata tggcggttgt
     6661 cgcatactgc catattgttg cggataagcc cttttgtgcg cgcgcccaag ggttcggtgg
     6721 caataataaa ggtgctgacg gcaatcgcct tgcgttccaa aggccggaat atcgggttca
     6781 aaccgacata agtattgacg gcatagacca catttttaca ctcgacgctg ccttcgggcg
     6841 tgtaaaccag ccaaccgttt tgatacggtt cgatgcgcgt catcggggat tgctcgaaaa
     6901 tctgcgcgcc ggcttcggca gcggcgctgg caacacccaa cgtgtaattg agcggatgaa
     6961 gatgcccgga caagggatcg aactgtgcgc cttggtacat atcgctgtca agctgctgtt
     7021 tcaactcggc tttatcccaa agttgataat gactcgcacc gtaatgccgt tgggcgtgtt
     7081 catgccactg ctgcaactct tcccaatgct gcggacggac ggcaaccgtg gcataaccgc
     7141 gctgccaatc gcaatcgatg gcatgtttgc ggacgcgttc gtccaccagt tcgaccgcct
     7201 gcaaagactg ttgccaaaac cattgcgcct gctccaagcc gacctgtttt tcaatttccc
     7261 ccataccgca ggcgtagtcg ctgataacct gcccgccact cctgccggac gcgccgaagc
     7321 cgatacgtgc ggcttccaaa acgacggctt catgtccgtg ttccgccagc ggcaatgcgg
     7381 tacacaaacc gctcaaaccg ccgccgataa tgcaggtttc ggctttcaga cggcattgga
     7441 gtttcggata aacagtatgc ggattaaccg aactaaaata ataagaaggc agatattctt
     7501 gaaaatcagg gcgaatcatt gtgtttgctt tatcgggtat attttcggac ggaatgatac
     7561 aggctgtcgg gccatatcgt ccaaacagaa aatcggttga aaaaaaacag gctgacccag
     7621 tcatgcggtc agcctgcctt attaattaat ttgctttctc ggcagccaat ttttcttggc
     7681 ggtaggcttc tgccgcttct cggttgcgct tggttgcctg cctcatcatc caataattga
     7741 cgatgatgac caatgttccg atgatgccga tcaggatggt tgccaagaca ttcatctgag
     7801 gatcgagacc caacttgatc ttggagaaaa taacctgcgg caatgtggat gaaccggggc
     7861 cggagaggaa tgaggtaatc accaaatcat ccaaagacaa ggtaatgccg agtaggaagc
     7921 ctgaagcaat ggcaggggca atcaaaggca aagtgatgac aaaaaagatt ttcagcggac
     7981 gcgcgcccaa gtccattgcg gcttcttcga gcgactggtc aagctcgacc agccgcgaac
     8041 ggatgacaac ggtaatgtat gccatgcaca gcgtcgtatg tccgaggaag atggtgaaaa
     8101 agccgcgatc gaagtagaga tgttgcaacc attcgctgcc ctgcaaaaat atctgcacct
     8161 gaataatcag cagcagcata gacagaccgg taatcacgtc gggcatgacc atgggcgcgg
     8221 aaatcatacc ggcaaacagc gtgctgccgc gaaagcgttt gatacgcgcc atcgcatagc
     8281 ccgccagcgt gcccaaaacg acggcggcaa gcgaagacac aacggcaatc cgcagcgaca
     8341 gccaagcggc ttccaaaatg gtgtcgtttt ccagcaatgc gccgtaccac ttggtcgaaa
     8401 agccgcccca aacggtaaca agtttggatt cgttgaacga atagatgacc aaaacaacca
     8461 gcgggatata cagaaacgcc agcgatagtg ccaacatcag tttcaagaac caagataatt
     8521 tggatttctg cattatttgg ctccttcttc caattcgcgg ttttcatagt gctggaacag
     8581 ggcaatcggt acaaccagta gcgcgaccat cacgacggcg acggcagaag ccaacggcca
     8641 gttgttttga tcaaagaatg cctgccacaa tactttaccg atcatcaggt tttccgaacc
     8701 gccgaccagc tcgggaatga cgaactcacc gacagcgggg acgaaaacca gcatcgaacc
     8761 tgcaataatg ccggttttcg acaaaggcag ggtaatcgtc aagaacgatt tgaccggtcc
     8821 cgcgcccaag tcggaagccg cttcgagcag gcggctgtcc agtttcacca gctgcgtgta
     8881 aagcggcaga atcataaacg gcagataggc ataaaccatt accaaattta acgaaaacgc
     8941 attatagaac aaatctaatg gttctctgat aatacccatc ttaatcaata agttgtttat
     9001 gatgccgtta tgcccgagca aacccatcca cgcatagaca cgcagcagga acgatgtcca
     9061 aaaaggtaac atgatggcaa gcagcaaacc attgcggacg gaaggattgg cacgggaaat
     9121 cgcataggcg gtcgggtaac cgaccaacag gcaaatcacc gtcgtcgtca gcgcagtctt
     9181 aattgaagac caataagtca tcagatagat attactgttt tcactgtcgc cgaaggggtt
     9241 aagggtatgc caaaaatttt ggaaaatatc ggcatagttt tgatagctga cggcgatgtt
     9301 caggcgaccc aagtcctcat ctatcgtcgt taaaggagta aacggtggaa tggcgatttc
     9361 ttgttcggca gagctgattt tcagcacgat ggcgaacgga atcagaaaca gcaccaaaag
     9421 ccaaatatac ggtacggcaa tcaccgcacg ctgccccgga cggcggaaca gtttgttttt
     9481 cagtttatta aggttcatct cactcccctt aaatcaacgg aacaacggag tcggttggtt
     9541 ttccggccag ctgatataga cggtttcatc ccaagtcggc ggcgtgatgt tacgcacata
     9601 ccagtaaggt gcaggaactt ggcttttgac gacgcgcccg ttggcgagtt tgatatggta
     9661 tatggcgaag ctgcccaaat aggcaatttc ttttaccgtg cctttcgccc agttgtagtc
     9721 gcccaaatat tcgggttttt ctttatataa atcaatatcc tctggtcgaa tactaaccca
     9781 aagatcctgc tcgctcgggc cacccaaacc atggtcgata cggacgtggt tttccaaacc
     9841 ttcgcattcg ataacggcat agtcggcatg attttcaatc accacaccgt caaagatgtt
     9901 ggtttcgccg ataaactcgg cagtgaagcg gctgttggga tagtcgtaca cgtcgctggg
     9961 cgtgccgacc tgctgcaact gaccgtcgga cataatggcg atgcgggtcg ccatcgtcat
    10021 cgcctcttct tggtcgtgcg taaccataat gcaggttacg ccgacttgtt ccagcgtatt
    10081 gaccaactcg agctgggttt gctggcgcaa tttcttatcc aacgccccca aaggttcgtc
    10141 aagcaacagg attttcggac gttttgccaa actgcgcgcc aaggcgatac gttgctgctg
    10201 accaccggac aattggtgcg gtttgcgttt ggcaaatttg gtcatctgaa ccagacgcag
    10261 catttcttcg acgcgcgcat cgatttcgcc tttaggcatt ttgtcctgtt tcagaccgaa
    10321 ggcaatgttt tgctccaccg tcatatgcgg aaaaagcgcg taactttgga acatcatatt
    10381 gatggggcgg tcatagggtg caagtttggt aatatcttga ccatcaagga tgatttttcc
    10441 ctgattggga ctttccatac ccgccagcat acgcagcagc gtagattttc cgctgccgga
    10501 actgcccaaa agggcgaaga tttcgttttg ataaatgttc aagtcgatgt tatcgacagc
    10561 gtaattgtca ccaaactttt tcaccaaacc ttggatttgc agataaggtt tggctgaaga
    10621 cgctgtggtt gcggtcataa tggcaatact ccaataaaaa gacgagtacc ggcaaaacgg
    10681 attttcgaat gggtgataaa aagctgtttg attgctggcg ggagtcgcaa cgtctgatgc
    10741 cgtctgaaac tcttgtgaag cgcacgggca gcatgaaata aaaaggggtt ctaaagaacc
    10801 ccgcattata ttagtttata aggtttttag ggtaataaat atgggtttga gcttgtcgga
    10861 aagcaacaga aaagaaaaca ccgcccattt ttctgggcgg tgtcggaaag cgcaattatt
    10921 tacgcagacc caagcgggta atcaacgcgc gatacgtatc gggctgggta cggcgcaaat
    10981 aggccaacag gcggcggcgt tggctgacca ttttcaacag gccgcgacgg ctgtggtggt
    11041 ctttggggtt ggctttgaag tggggggtca gatcgttgat gcggaaagtc aacagggcaa
    11101 cttgtacttc agaagagccg gtgtcgcctt ctttacgttg gaaatcttta acgatttgtg
    11161 ctttttgttc tacggtcagt gccataatgg aaaaactccg aaaaataaaa agaaccccaa
    11221 atgggattcg gacaagtttg ccaagcctcg acaacggcaa actccctatt cctgaaacag
    11281 gaacagcgca ttatcacaca atttttgctg ttctgcacga tattttgcga tgtcggcgaa
    11341 atgccgtctg aaaacggaga catcttccgg tttcagacgg catttgacat cagaccacaa
    11401 tttcttcctt ctgtttcgct tccttggcga tattgtcgcg gctcaaaccg aacatcagca
    11461 agagcgggct ggcaaccaat acggaagagt agatgccgaa cacgataccg atggtcagtg
    11521 ccatagaaaa gccgtgcaag gctgcaccgc cgaacaccag catagaaaca accatcgcct
    11581 cggtcgaacc gtgggtaatg atggtgcggc tcatcgttgc ggtaatcgcg ttgtcaatga
    11641 cttccggcac ggtatgtccg cgcatcgccg gcttgcggaa attttcacgg atacggtcga
    11701 agacgacgac ggattcgttc acagaatagc ccaatacggc aaggatacct gccaagacgg
    11761 tcagcgaaaa ttcccattgg aagaaggcaa agcagccgag aataatcacg atgtcgtgca
    11821 tattggcgat aatggcagat acggcaaaac gccattcgaa acgcatcgac aggtaaataa
    11881 tgatgccgat aacgacaaaa cctaaagcca tcaatccatt atttaccaat tcctcaccga
    11941 cttgcgggcc gataaattcg acttggcgca aagtaacgtc gggactgtct tttttcagca
    12001 aatccataac ctgattggac aactgtgcgg aagtaacacc ttctttgttc ggcaggcgga
    12061 tcatgatgtg tttgttcgta cccaatgcct gaacctgtac atcacccatt ttcagcgtat
    12121 cgaggcgttc gcgcatctta ttgacatccg caccctgctg atattggact tccattaccg
    12181 taccgccggt aaattcgaca gagaaattca ggcctctggc aaccaaaaag aatacggcgg
    12241 caataaaggt aaccaacgaa atgaaggttg tcagtttgcc gtagctcata aacggaatat
    12301 cgcgtttgat tttaaagagt tccatagctt actccttacc tgccgcagtt tcggcttcag
    12361 gtttccatac cgcaccgatg gaaatattct gcaatttgcg gcggcggccg taccacagat
    12421 tgaccaacgc acggaatacg acgacggacg aatacatcga agtcagaata cccaagcagt
    12481 gtacgacggc aaaacctctt accggtccgg aaccgaatac caaaagcgcg ataccggcaa
    12541 tcagcgaagt caggttcgaa tcaacaatgg tcgcccatgc gtgttggaaa ccgagattga
    12601 ttgcctgctg cggcggcacg ccggcacgca attcctctcg gatacgttcg ttaatcaaga
    12661 cgttggagtc gattgccata cccaaagtca acgccagcgc ggccataccc ggtaacgtca
    12721 acgttgcctg catggcagac aaaataccga ttaggaacag tatgttggca ctcaatgcaa
    12781 tagtagaaaa gaaacccatc aggcggtaat aaaccaccat gaatgcagca acgatggcaa
    12841 aaccccataa agtcgaatgg aagccttttt cgatgttctc cttacccaaa gacggaccga
    12901 tggtacgttc ttcgacaatc tgcatcggtg cggcaagaga accggcacgc aacagcaaag
    12961 acgtatcgtt ggcttcggct gtcgtcatgc ttccggaaat ttccacgcgt ccgccggtaa
    13021 tggcagtacg gataaccggt gcggttacaa cctcggattt tccttggtcg atcaaaacca
    13081 tcgccatgcg tttgccgaca tttgcggcag tcagttcgcc gaaaatgctg ccgcccgcgc
    13141 tgtccaagct cagactgacg gcaggtgcgc ccatttggtc gaaactcggt tgcgcatcgt
    13201 tgatgttgtc gccggtcagc tcgacctgtt tgctgatcag cagagtttcg ggatgctccc
    13261 cgccgcttga aagcagctca taaccgctcg gcacgttgcc ttccaatgcc tcgcgcaact
    13321 tggcaggatc gtcctccacc atgcggactt ccaaagtcgc ggtacggccg atgatgtctt
    13381 ttgccttggc agtatcttgc acgccgggaa gctgtacgac aatgcggtcc aaacctgact
    13441 gttggataat cggctctgcc acacccaatt cgtttacacg gttatgcagt gtagtgatgt
    13501 tttgcttcac cgcatctgat cgtactttat tcaccgcctc ttccgaaagc gccaatacaa
    13561 tattgctgcc ttctgaattt aacgttgcct caggaaacag cttgagtaac tgaggcagtg
    13621 ccttttgcac atcgccggca tcctgcaaag ggacggtcag gctgttttca gcctgacgca
    13681 ccgtgccgct gcggattttt tcacggcgca gttcgcggcg gatgtcgccc gaataacgct
    13741 caaacgtttt ctgcatcgcg gctttcatat cgacctgcat ggtgaaatgc acgccgccgc
    13801 gcaggtccaa acccaaaaac atcggattgg ctttgatttt cgccatccat tcggggctgt
    13861 ccgccaacag gttgagcgcg gtaatatacc cttcgcccaa agtgttttcg ataacgtcgc
    13921 gcgctttaag ctgcgtttct gtgtctttga aacgcacttt cagtgaatta tccacaacaa
    13981 acatcccgtc ggtctgaatg cccgcgtttt tcagcgcagc atccactttg gattgagtct
    14041 gttcgttgat gatgatggct tgtcggttgg tcgatacctg cacggcgggc gtttcgccga
    14101 ataggttggg cagcgaatac actgcggcaa ccgcaatcgt gaacacaatc agcagatatt
    14161 tccataaagg ataacggttc atcattgttc cttaatggtt ggaaccccac cctttcggtg
    14221 gtgtcggaat cgggctattt cagaagaggc aaaaacccct ccctgccagg caagaccgaa
    14281 aggcagcatc ctgaatatgc cgccctgcgt gtcggaacat ggtcaagcct tcggttggaa
    14341 ttcaaaacaa agtgccgcat tcgggctttc ccgatgcggc ttgtcggcac aaatcaatcg
    14401 acttttgcgg caatcgcatt gcgttccact tcgacctcga tttttgtacc ctgtccgata
    14461 tccacggtaa aaaactgttc gccgactctg gttaccttac ccttgaaacc tgccgccaag
    14521 accactttgt cgccggcttt caaggcggca agcattgcct gatgcgcttt gaatttcttt
    14581 tgttgggggc gcatgatcag gaagtagaac accaccataa tcaacaccaa aggagcaaat
    14641 tgtgcaacag cttgattcat aatttatccg ttctttctaa atatgattga aaatcgagcg
    14701 gggtatataa tagcataaga ccgtaaacaa tatatcgggt ttgccgccca tacccaaccg
    14761 tatgccgtct gcaccccctt tcccgaaccc atgccgctga cccgcctgtt cctgaaactc
    14821 tacgccctgt tgcgcctttt tttgggcaaa aacgcccaga ccgcctggat tttccacccc
    14881 gcctgcaccg ggcacgaccc cggggcaaac catcccgatt cgcccgaccg catcctctgc
    14941 atcgagcagg cattgcgccg cgccggtatt tggcagcacc tccaaaccgt agaggcggaa
    15001 gaaatcagcg atacgcgcct tgcacttgtc cgctcgagca aatatctgaa ccgtttggaa
    15061 tcttgcctac ccgaagacgg caagatttgc cgtttggacg acgacaccgt tatcagcaaa
    15121 aactccctgt ccgccgcccg tttttccgcc ggttcggcag ttcaggcagt cgatatggtc
    15181 atgaaacgca aagcgtggca cgccttttgc gccgcccgcc cgccggggca tcacgcaaaa
    15241 agcggcaaag ccggcggatt ctgcctgctg aacaacgttg ccgccggcgt catgcatgcc
    15301 attgccgaat accgcctgaa acgcattgcc gtcatcgatt tcgatgtcca ctacggcgac
    15361 ggtacggcag aaatattcaa agacgatccg cgcatcctgt ttttcaacct gtttgaaacc
    15421 gaccttttcc ccttccccga aaacaacggt atgcccgacg gcggcaatat ggtgcacctg
    15481 cccttgccgt cgggaacggg cagccgcaca ttccgcgaag ccgtccgcag acagtggctg
    15541 ccccggcttg ccgcattcaa acccgaactg gtgctgctgt cggcaggatt cgacgcacac
    15601 cgtctggacg aatcgggcag gctcaacctg cacgaggcgg attttgcctg gctgacacac
    15661 aaaatcattc agacggcatc cagctgcccc ggcaaaatcg tatccgtact cgaaggcggc
    15721 tacacccttg aacctctggc acaatctgct gccgagcata tccgcgtact ggcggggctg
    15781 ggcaaatccg atgccgcaac cgcctatcag aaaacactga acccaacaaa aaaacggttt
    15841 gccaaaccga aaaccggaca ggtgcgccaa cctacccaat ccgaccgtta cgatataaat
    15901 gccgtctgaa gccgattcgg ttttcagacg gcatcattat ttgacctgac gcaaacagaa
    15961 agccttccgc gtgcttaaac acatctcttg agacctttgc aaaaatcccc
//
LOCUS       DAWTQS010000051        15598 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.051, whole genome shotgun sequence.
ACCESSION   DAWTQS010000051 DAWTQS010000000
VERSION     DAWTQS010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15598
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.051"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            195..1538
                     /locus_tag="WNA25_001547"
     CDS             195..1538
                     /locus_tag="WNA25_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HFA9824270.1"
                     /translation="MIKIKKGLNLPIAGRPEQVIYDGPAITEVALLGEEYVGMRPSMK
                     IKEGEAVKKGQVLFEDKKNPGVVFTAPASGKIAAIHRGEKRVLQSVVIAVEGNDEIEF
                     ERYVPEALAKLSSEEVRRNLIQSGLWTALRTRPFSKIPAVDAEPFAIFVNAMDTNPLA
                     ADPTVIIKEAAEDFKRGLLVLSRLTERKIHVCKAAGADVPSENAANIETHEFGGPHPA
                     GLSGTHIHFIEPVGANKTVWTINYQDVIAIGRLFVTGRLNTERVVALGGLQVNKPRLL
                     RTVLGAKVSQLTAGELVDADNRVISGSVLNGAIAQGAHDYLGRYHNQISVIEEGRGKE
                     LFGWVAPQPDKYSITRTTLGHFLKNKLFKFTTAVNGGDRAMVPIGTYERVMPLDILPT
                     LLLRDLIVGDTDSAQALGCLELDEEDLALCSFVCPGKYEYGPLLRKVLETIEKEG"
     gene            1541..2773
                     /locus_tag="WNA25_001548"
     CDS             1541..2773
                     /locus_tag="WNA25_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HFA9824271.1"
                     /translation="MGLKHFLEKIEPHFLPGGKHEKWYVLYEAAATIFYTSGAVTRKA
                     AHVRDALDSKRMMILVWLALFPAMFYGMYNVGAQAFGALTPDLLQQSIANDWHYALAN
                     ALGINMSPEAGVLGKMLFGAIYFLPIYATVFIVGGFWEVLFASVRKHEINEGFFVTSI
                     LFALIVPPTLPLWQAALGISFGVVVAKEVFGGTGKNFMNPALAGRAFLFFAYPANLSG
                     DAVWTAVDGYSGATALAQWAAHGADGLKNAVTGQTITWMDAFIGKLPGSIGEVSTLAL
                     LIGGAFIVFARIASWRIIAGVMIGMIAMSSLFNFIGSDTNAMFAMPWYWHLVVGGFAI
                     GMLFMATDPVSASFTNVGKWWYGALIGVMCVLIRVANPAYPEGMMLAILFANLFAPIF
                     DYFVAQANIKRRKARSNG"
     gene            2766..3542
                     /locus_tag="WNA25_001549"
     CDS             2766..3542
                     /locus_tag="WNA25_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HFA9824272.1"
                     /translation="MAKKFDKDSFSGTLIVVLAVSLICSVIVAGAVVGLKPIQEKQKL
                     QDKQGYILSVAGLMDKDTDIGKTFAERIEQRVVDLATGEYVKDAPKDFSARIAGKDPA
                     QSIRIKPEDDLAGIKSRAKYTEVYLVKGEDGKIGQIILPMHGNGLWSVMYGFVAIRPD
                     GNTINGITYYEQGETPGLGGEIGNPLWQQKFVGKKLFDGQGKLALHVGKGAGSDKEHG
                     VDALSGASLTSKGVQGSFAYWFGENGYIPYLNKLKSAGVQ"
     gene            3542..4168
                     /locus_tag="WNA25_001550"
     CDS             3542..4168
                     /locus_tag="WNA25_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HFA9824273.1"
                     /translation="MADMKRLKHLMFSPFIDNNPIALQVLGICSALAVTTKLQTAIVM
                     GISVALVTGFSSFFISLVRNYIPNSIRIIVQMAIIASLVTLVDQLLQAFAYELSKQLS
                     VFVGLIITNCIVMGRAEAFAMKEPPLESLIDGIGNGAGYGMMLLVVATVRELIGSGKL
                     LGYTVFQTVQDGGWYQTNGLFLLAPSAFFIIGFLIWGLRTWKPEQAEE"
     gene            4172..4765
                     /gene="nqrE"
                     /locus_tag="WNA25_001551"
     CDS             4172..4765
                     /gene="nqrE"
                     /locus_tag="WNA25_001551"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HFA9824274.1"
                     /translation="MEHYLSLFIKSVFIENMALSFFLGMCTFLAVSKKVSTAFGLGVA
                     VIFVLGLSVPANQLVYSLLKDGAIVEGVDLTFLKFITFIGVIAALVQILEMFLDKFVP
                     ALYNALGIYLPLITVNCAIFGAVSFMAQREYDFGESVVYGFGAGLGWMLAIVALAGIT
                     EKMKYSDAPKGLKGLGITFIAAGLMAMAFMSFSGIQL"
     gene            4779..5996
                     /gene="nqrF"
                     /locus_tag="WNA25_001552"
     CDS             4779..5996
                     /gene="nqrF"
                     /locus_tag="WNA25_001552"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HFA9824275.1"
                     /translation="MEIILGIVMFTVIVLALALMILFAKSKLVSEGDITIKVNDEKEL
                     TMPAGGKLLGALASQGIFVPSACGGGGSCGQCRVVVKSGGGDILPTELSHISKREARE
                     GCRLSCQVNVKTDMDIEVPEEVFGVKKWECTVISNDNKATFIKELKLAIPEGEEVPFR
                     AGGYIQIEAPPHTVAYKDFDIPKEYHEDWDKYNLWQYVSKVDEPILRAYSMASYPEEK
                     GIIMLNVRIATPPPRVPDAPPGQMSSYIWSLKPGDKVTISGPFGEFFAKDTDAEMVFI
                     GGGAGMAPMRSHIFDQLKRLHSKRKITFWYGARSKREMFYVEDFDQLAAEFPNFTWHV
                     ALSDPLPEDNWDGYTGFIHNVVYENHLKNHEAPEDCEFYMCGPPIMNQSVIKMLKDLG
                     VEDENILLDDFGG"
     gene            6513..7568
                     /locus_tag="WNA25_001553"
     CDS             6513..7568
                     /locus_tag="WNA25_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFA9824276.1"
                     /translation="MPSETRLPNLIRALIFALGFIFLNACSEQTAQTVTLQGETMGTT
                     YTVKYLSNNRDKLPSPAKIQKRIDDALKEVNRQMSTYQTDSEISRFNQHTAGKPLRIS
                     SDFAHVTAEAVRLNRLTHGALDVTVGPLVNLWGFGPDKSVTREPSPEQIKQAASYTGI
                     DKIILQQGKDYASLSKTHPKAYLDLSSIAKGFGVDKVAGELEKYGIQNYLVEIGGELH
                     GKGKNAHGEPWRIGIEQPNIIQGGNTQIIVPLNNRSLATSGDYRIFHVDKNGKRLSHI
                     INPNNKRPISHNLASISVVSDSAMTADGLSTGLFVLGETEALRLAEQEKLAVFLIVRD
                     KDGYRTAMSSEFAKLLR"
     gene            7594..7809
                     /gene="nqrM"
                     /locus_tag="WNA25_001554"
     CDS             7594..7809
                     /gene="nqrM"
                     /locus_tag="WNA25_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HFA9824277.1"
                     /translation="MKTLLLTFGIFLTVIIGMAVGYIFSKRTIKGSCGGITALGMKKM
                     CDCDTPCDTLQKKLDEEKQAGGIRVDR"
     gene            complement(7933..8511)
                     /gene="grpE"
                     /locus_tag="WNA25_001555"
     CDS             complement(7933..8511)
                     /gene="grpE"
                     /locus_tag="WNA25_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFA9824278.1"
                     /translation="MSEQTQQQNSEEAVENVEAVETVETVGNADGVQEQAAAEPAYED
                     LQARIAELEAQLKDEQLRALANEQNLRRRHQQEIADTHKFAGQKFAVEMLPVKDYLEM
                     ALLDQSGNFDALKMGVQMTLNELQKAFDATQIKEINPKAGDKLDPNIHQAMQAVASEQ
                     EPNTVVGVMKKGYTLSDRVLRPAMVTVAQKEA"
     gene            8694..9512
                     /gene="cysE"
                     /locus_tag="WNA25_001556"
     CDS             8694..9512
                     /gene="cysE"
                     /locus_tag="WNA25_001556"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFA9824279.1"
                     /translation="MKKNHLNTTGFDLWHTIREETAAAAAAEPMLASFLHQTVLRHES
                     LGSVLAYHLSSKLGSPIMDVRALFEIYQQALGSDTQISKCVEADLKAIYERDPACDEY
                     SLPLLYFKGFHAIQAHRINHRLYLDGRKTLAYFLQNRMSEVFGVDIHPAARLGYGLML
                     DHATGFVAGETAVLGNNISILHGVTLGGSGKEGGDRHPKIGDGVMIGANASILGNIRI
                     GSNAKIGAGSVVVSDVPPSITVVGVPAKPVARSLKTPSADMDQNIQFAEIDFMI"
     gene            complement(9716..11227)
                     /gene="ubiB"
                     /locus_tag="WNA25_001557"
     CDS             complement(9716..11227)
                     /gene="ubiB"
                     /locus_tag="WNA25_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFA9824280.1"
                     /translation="MKWLKRLTVIVGTFYRYRLAGLCVSLMGSGWICALLKMMPQSSK
                     LKNEPPAVRLRLALESLGPIFIKFGQVLSTRPDLIPHDYAVELAKLQDKVPPFDARLS
                     REQIEKSLGQPIEKLYAEFETEPVASASIAQVHKARLHSGERVAVKVLRPNLLPLIEQ
                     DLSLMRFGAAWVERLFSDGKRLKPREVVAEFDKYLHDELDLMREAANAGQLGRNFQNS
                     NMLIVPKVFYDYCTSDVLTIEWMDGTPVSDIAKLKADGIDLHKLADYGVEIFFTQVFR
                     DGFFHADMHPGNILVAADNRYIALDFGIVGTLTDYDKRYLAINFLAFFNRDYRRVATA
                     HVESGWVPADTRAEELEAAVRAVCEPVFNKPISQISFGLVLMRLFEVSRRFNVEIQPQ
                     LVLLQKTLLNIEGLGRQLDPDLDLWKTAKPFLVKWMNGQVGPKALWRNLKNEAPDWAQ
                     IIPSLPRKINALVDEARRQEMRDAYIHLVKVQQRQSLWLAAIAVVLLLILLLK"
     gene            complement(11262..11471)
                     /locus_tag="WNA25_001558"
     CDS             complement(11262..11471)
                     /locus_tag="WNA25_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease III domain protein"
                     /protein_id="HFA9824281.1"
                     /translation="MELETVVPPEEGEGEGGGADGEMPEYLELHGGREDDISASSQPE
                     PPRNIKPLVFGLLIAFLGYLLGKIF"
     gene            complement(11716..11862)
                     /locus_tag="WNA25_001559"
     CDS             complement(11716..11862)
                     /locus_tag="WNA25_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824282.1"
                     /translation="MPSERSDGIFTFGKYIIGMNLHIIRLLEDESGFPKGTGGTVSGV
                     FVPL"
     gene            complement(11879..12217)
                     /gene="erpA"
                     /locus_tag="WNA25_001560"
     CDS             complement(11879..12217)
                     /gene="erpA"
                     /locus_tag="WNA25_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFA9824283.1"
                     /translation="MSDESPIIFTDSCCAKVADLIAEENNPDLKLRVFVNGGGCSGFQ
                     YGFTFDEIKNDDDFEIEKNGLVFLVDPMSYQYLVGAEIDYTESLQGSQFVIRNPNAET
                     TCGCGSSFSV"
     gene            complement(12350..13036)
                     /locus_tag="WNA25_001561"
     CDS             complement(12350..13036)
                     /locus_tag="WNA25_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFA9824284.1"
                     /translation="METFKDRLVFLWKSEARQAKIASDIEMTIAGFSRIWNEGGLPKS
                     ETLKKIKQLKGCSIDWLLTGEGNPFPDEAPKKSLAYDTLGNEVDTDEFVFVPRYDIRA
                     AAGYGQFVGHEEPVFTMAFRRHWIENYVTRDTKNLSVISVKGDSMEGVLNDGDSILVN
                     HGENTPRDGLYVLRINENLLVKRLQIVPGGIINVISANEAYPAFEINLNDLTDDVEII
                     GRVEWFGRTV"
     gene            13223..13495
                     /locus_tag="WNA25_001562"
     CDS             13223..13495
                     /locus_tag="WNA25_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824285.1"
                     /translation="MKKTSKYLIYTAAFTSFCFAFQENRSEAKQPDITLSASLCEQFN
                     MLNAKDMDAEQVSLSKECDIIESSHDWEKEYGNLNEQEMLAGVVYE"
     gene            13703..>15598
                     /gene="dnaK"
                     /locus_tag="WNA25_001563"
     CDS             13703..>15598
                     /gene="dnaK"
                     /locus_tag="WNA25_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFA9824286.1"
                     /translation="MAKVIGIDLGTTNSCLAISENGQTKVIENAEGARTTPSIIAYLD
                     GGEILVGAPAKRQAVTNAKNTIYAAKRLIGHKFEDKEVQRDIESMPFEIIKADNGDAW
                     VKAQGKELSPPQISAEVLRKMKEAAEAYLGEKVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAFGMDKGDNKDRKIAVYDLGGGTFDISIIEIANLDGDKQFE
                     VLATNGDTFLGGEDFDQRLIDYIIDEFKKEQGIDLKQDVMALQRLKEAAEKAKIELSS
                     GQQTEINLPYITMDATGPKHLAMKITRAKFESLVEDLIARSIEPCRTALKDAGLSTGD
                     IDDVILVGGQSRMPKVQEAVKDFFGKEPRKDVNPDEAVAVGAAIQGEVLSGGRSDVLL
                     LDVTPLSLGIETMGGVMTKLIQKNTTIPTKASQVFSTAEDNQSAVTIHVLQGERERAS
                     ANKSLGRFNLGDIAPAPRGMPQIEVTFDIDANGILHVSAKDKGTGKAANITIQGSSGL
                     SEEEIERMVKDAEANAEEDKKLTELVASRNQAEALIHSVKKSLADYGDKLDAAEKEKI
                     EAALKEAEEAVKGDDKTAIDAKAEALGTASQKLGEMVYAQAQAEAQAGEGAQANASAK
                     KDDDVVDA"
BASE COUNT         4039 a         4319 c         3682 g         3558 t
ORIGIN      
        1 caaatcaata tcccccttcc gacaattaaa aacggcttcc gcaaggaaaa aagcggcaaa
       61 tctgacaaac aggtacaatt cctgataaaa tctaacactt tgctacatta atttattcaa
      121 tgcttttgat gccacgcgac acggtatcaa aaacacgccg tttttacctt aaccatcaca
      181 agtaatgcaa atctatgatt aaaatcaaaa aaggtctaaa tctgcccatc gcgggcagac
      241 cggagcaagt catttatgac ggcccggcca ttaccgaagt cgcgttgctt ggcgaagaat
      301 atgtcggcat gcgcccctcg atgaaaatca aggaaggtga agccgtcaaa aaaggccaag
      361 tgctgtttga agacaaaaag aatccgggcg tagtatttac tgcgccggct tcaggcaaaa
      421 tcgccgctat tcaccgtggc gaaaagcgcg tacttcagtc agtcgtgatt gccgttgaag
      481 gcaacgacga aatcgagttc gaacgctacg tacctgaagc gctggcaaaa ttgagcagcg
      541 aagaagtgcg ccgcaacctg attcaatcag gcttatggac tgcgcttcgc acccgtccgt
      601 tcagcaaaat ccctgccgta gatgccgagc cgttcgccat cttcgtcaat gcgatggaca
      661 ccaatccgct ggctgccgac cctacggtca tcatcaaaga agccgccgaa gacttcaaac
      721 gcggcctgtt ggtattgagc cgcctgaccg aacgtaaaat ccatgtgtgt aaagcagcag
      781 gcgcagacgt gccgtctgaa aatgctgcca atatcgaaac acatgaattt ggcggcccgc
      841 atcctgccgg cttgagtggc acgcacattc atttcatcga gccagtcggc gcgaataaaa
      901 ccgtgtggac catcaattat caagacgtga ttgctatcgg acgtttgttc gtaacaggcc
      961 gtctgaatac cgagcgcgtg gttgccttgg gcggcctgca agtcaacaaa ccgcgcctct
     1021 tgcgtaccgt tttgggtgcg aaggtgtctc aacttaccgc cggcgaattg gttgacgcgg
     1081 acaaccgcgt gatttccggt tcggtattga acggtgcgat tgcacaaggc gcgcatgatt
     1141 atttgggacg ctaccacaat cagatttccg ttatcgaaga aggccgcggc aaagagctgt
     1201 tcggctgggt tgcgccgcag ccggacaaat actccatcac gcgcaccact ctcggccatt
     1261 tcctaaaaaa caaactcttc aagttcacga cagccgtcaa cggcggcgac cgcgccatgg
     1321 taccgatcgg cacttatgag cgcgtaatgc cgttggacat cctgcctacc ttgcttttgc
     1381 gcgatttaat cgtcggcgat accgacagcg cgcaggcttt gggttgcttg gaattggacg
     1441 aagaagacct cgctttgtgc agcttcgtct gcccgggcaa atacgaatac ggcccgctgt
     1501 tgcgcaaagt gctggaaacc attgagaagg aaggctgatt atgggcttaa aacattttct
     1561 ggaaaaaatc gaaccgcact tcctgccggg cggcaaacat gaaaaatggt atgtcctcta
     1621 tgaagctgcg gcgacgattt tctatacatc cggcgcggta acgcgcaaag cggcgcacgt
     1681 ccgcgacgcg ctcgactcca aacgcatgat gattttggtg tggctggctt tgttccccgc
     1741 catgttttac ggcatgtaca acgtcggcgc acaggcattc ggtgccttaa cgcccgattt
     1801 gctgcaacaa agcatcgcca acgactggca ttacgccctt gccaacgctt tgggcatcaa
     1861 tatgtcgcct gaagcgggcg tgttgggcaa aatgctgttc ggcgcgattt acttcctgcc
     1921 gatttacgcg accgtattta ttgtgggcgg cttctgggaa gtcttgttcg catccgtacg
     1981 caaacacgaa atcaacgaag gtttcttcgt tacttcgatt ctgtttgcct taatcgttcc
     2041 gcccacgctg ccgctgtggc aggcggcttt gggtatttct ttcggcgttg tggttgcgaa
     2101 agaagtattc ggcggtacag gtaaaaactt catgaaccct gcgctggcag gccgcgcctt
     2161 cctgttcttc gcctaccccg ccaacttgag cggcgatgcg gtttggacgg cagttgacgg
     2221 ctattccggc gcaaccgcgc tggcgcaatg ggcggcacac ggtgcagacg gcctgaaaaa
     2281 cgccgtaacc ggtcaaacca tcacttggat ggacgcgttt atcggcaaac tgcccggctc
     2341 catcggcgaa gtctccactt tggcactctt aatcggcggc gcgtttatcg tgtttgcccg
     2401 catcgcttct tggcgcatta ttgccggcgt gatgatcggt atgattgcga tgtcttcgct
     2461 gtttaacttc atcggttctg acaccaacgc tatgtttgct atgccttggt actggcactt
     2521 ggtggtcggc ggcttcgcca tcggtatgct gtttatggcg accgaccccg tttccgcttc
     2581 ctttaccaat gtcggcaaat ggtggtacgg cgcgcttatc ggcgtaatgt gcgtgttaat
     2641 ccgcgtggcc aatccggctt accccgaagg catgatgttg gcgattctgt ttgccaacct
     2701 gtttgccccg attttcgact atttcgtcgc acaagcgaac atcaaacgca gaaaggcgcg
     2761 cagcaatggc taagaaattc gataaagaca gcttcagcgg cacgctgatt gtcgtgttgg
     2821 cggtcagcct gatttgctcg gtcatcgttg ccggtgcggt cgtcggcttg aaacccatcc
     2881 aagaaaaaca aaaactccaa gacaaacaag gctatatttt gagcgtggcc ggtttgatgg
     2941 ataaggacac cgacatcggt aaaacctttg ccgagcgtat cgagcaacgc gtggtcgatt
     3001 tggcgaccgg cgaatatgtg aaagacgcgc cgaaagactt cagcgcgcgc atcgcaggca
     3061 aagaccccgc gcaaagcatc cgcatcaaac ctgaagacga tttggcaggc atcaaaagcc
     3121 gcgccaaata caccgaggtt tatttggtaa aaggcgaaga cggcaaaatc gggcaaatca
     3181 tcctgccgat gcacggcaac ggtttgtggt cggtgatgta cggcttcgtc gccatccgac
     3241 ccgacggcaa caccatcaac ggcattacct actacgaaca aggcgaaacc ccgggcttgg
     3301 gcggcgaaat cggcaatcct ttgtggcagc aaaaattcgt cggcaaaaaa ttgtttgacg
     3361 gacaaggcaa actcgccctg cacgtgggca aaggcgcggg ttcggacaaa gaacacggcg
     3421 tagacgccct ctccggcgcg tcgctgactt ccaaaggcgt gcaaggttcg ttcgcctact
     3481 ggttcggcga aaacggctat atcccctacc tgaacaaatt gaaatcagca ggagtgcaat
     3541 aatggctgat atgaaacgct tgaaacattt gatgttctca ccctttatcg acaacaaccc
     3601 gattgccttg caggttttgg gtatttgttc ggctttggcg gttaccacca aacttcagac
     3661 ggccatcgta atgggtattt ccgtagcttt ggtaaccggt ttttccagct tcttcatttc
     3721 gctggtgcgc aactacatcc ccaacagcat ccgcatcatc gtgcaaatgg cgattatcgc
     3781 gtcgctggtt acgctggtcg accaactgct tcaggcattt gcctacgaat tgtccaagca
     3841 gctttccgta ttcgtcggcc tgattattac caactgtata gtgatgggcc gtgccgaagc
     3901 atttgcgatg aaagagccgc cgctggaaag cctgatcgac ggcatcggca acggtgcggg
     3961 ctacgggatg atgctgcttg tcgtcgccac cgtccgcgaa ctgatcggct caggcaaact
     4021 tttaggttat accgtctttc aaaccgtaca ggacggcggc tggtatcaga ccaacggcct
     4081 cttcctactc gcccccagcg cgttcttcat catcggcttt ttgatttggg gactgcgtac
     4141 ttggaaaccc gaacaggcgg aggaataagc tatggaacac tatttgagcc tcttcatcaa
     4201 atccgtcttc attgaaaata tggcgctatc cttctttttg ggtatgtgta cgtttttggc
     4261 ggtatccaaa aaagtatcca ccgcattcgg tttgggtgtg gcggtaattt tcgtactcgg
     4321 gctgtccgtc cccgccaacc aattggttta ctcgctgctc aaagacggcg cgattgtcga
     4381 aggcgtggat ttgacctttt tgaaattcat cacctttatc ggcgtgattg ccgctttggt
     4441 gcagattttg gaaatgttct tggacaaatt cgtccccgcc ctctataacg cgctgggcat
     4501 ctacctgcct ctgattaccg taaactgcgc gattttcggc gccgtatcgt ttatggcgca
     4561 acgcgaatac gacttcggcg agtccgtcgt gtacggcttc ggcgcgggtt tgggctggat
     4621 gttggcgatt gtcgctttgg cgggcattac cgaaaaaatg aaatattcgg acgctcccaa
     4681 aggcctcaaa ggcttgggca ttacctttat cgccgccggc ctgatggcaa tggcgtttat
     4741 gtcgttctcc ggcatccagt tataagaaag ggaccggtat ggagattatt ttaggtatcg
     4801 tgatgtttac cgtcatcgtc ttagctttgg cactgatgat tctgtttgcc aaatccaagt
     4861 tggtcagcga aggcgacatc accatcaaag tcaatgatga aaaagaactg actatgcccg
     4921 ccggcggcaa actcttgggc gcgcttgcca gccaaggcat ctttgtcccc tccgcctgcg
     4981 gtggcggcgg ttcgtgcgga caatgccgcg ttgtcgtgaa aagcggcggc ggcgacattc
     5041 tgccgaccga gctgtcccac atcagcaaac gcgaagcacg cgaaggctgc cgtttgtcct
     5101 gccaagtcaa tgtcaaaacc gacatggaca tcgaagtacc cgaagaagtg ttcggcgtga
     5161 aaaaatggga atgcaccgtc atttccaacg acaacaaagc aacgttcatt aaagaactca
     5221 agcttgccat tcccgaaggc gaagaagttc ctttccgcgc cggcggctac attcaaatcg
     5281 aagccccgcc gcacaccgtt gcctacaaag acttcgacat tcccaaggaa tatcacgaag
     5341 actgggacaa atacaatctg tggcaatacg tttccaaagt ggacgagccg attttgcgcg
     5401 cctactctat ggcttcgtat cctgaagaaa aaggcatcat tatgctgaac gtgcgtatcg
     5461 ccacgcctcc cccgcgcgta cccgatgcgc ctccgggaca aatgtcgtcc tacatctggt
     5521 cgctcaaacc cggcgacaaa gttacgattt ccggcccgtt cggcgaattt ttcgccaaag
     5581 acaccgatgc cgaaatggta tttatcggcg gcggtgcggg tatggccccg atgcgttccc
     5641 acattttcga ccagttgaaa cgtttgcact ccaaacgcaa aatcaccttc tggtacggcg
     5701 cacgctccaa acgcgaaatg ttctatgtcg aagacttcga ccaactcgcg gcagaattcc
     5761 cgaacttcac ttggcacgtc gccctgtccg acccattgcc tgaagacaac tgggacggct
     5821 acacgggctt catccacaac gtggtttacg aaaaccacct gaaaaaccac gaagcaccgg
     5881 aagactgcga attttatatg tgcggcccgc cgattatgaa ccagtccgtc atcaaaatgc
     5941 tcaaagactt gggcgtggaa gacgaaaaca tcctcttgga cgatttcggc ggttaaatat
     6001 tcggcttcaa acacaaaagg aagaaacccc ggtttcttcc ttttttatcc gatgccgtcc
     6061 gaacaccctt cagacggcat ccgcccatac ccgtatcaat atcgtttttt taccctgcgg
     6121 cggatgtgcg gctaaatatt ttgacgaaaa aacaaagaag tatacttctt tttagttatg
     6181 gttgattcca taactaaaaa agtatcgatc aacccatttt acgaggtacg gataatgtct
     6241 gacagctttg aaaacagccc cgaatacgat gattggattg aatcgggcgg tcgtgatgaa
     6301 gattacgaat actactacaa caaatggcaa cgcaggacgc gctgaccatt aaaccctgac
     6361 aaaccggttt gtaaaacacc ggtttgtttt ttattaccgc cacccaaacg acgggcgatg
     6421 ccgcccgaac gcccttcaaa cggcatcggc atagaaacat accggccgtt ttgcgatata
     6481 attcaagcca tttgccaaaa ccgtcaaaca ctatgccgtc tgaaacacgc ctgccgaacc
     6541 ttatccgcgc cttgatattt gccctgggtt tcatcttcct gaacgcctgt tcggaacaaa
     6601 ccgcgcaaac cgttaccctg caaggcgaaa cgatgggtac gacctatacc gtcaaatacc
     6661 tttcaaataa tcgggacaaa ctcccctccc ctgccaaaat acaaaagcgc attgatgatg
     6721 cgcttaaaga agtcaaccgg cagatgtcca cctaccagac cgattccgaa atcagccggt
     6781 tcaaccaaca cacagccggc aagcccctcc gcatttcaag cgatttcgca cacgttaccg
     6841 ccgaagccgt ccgcctgaac cgcctgactc acggcgcact ggacgtaacc gtcggccctt
     6901 tggtcaacct ttgggggttc ggccccgaca aatccgttac ccgtgaaccg tcgccggaac
     6961 aaatcaaaca ggcggcatct tatacgggca tagacaaaat cattttgcaa caaggcaaag
     7021 attacgcttc cttgagcaaa acccacccca aagcctattt ggatttatct tcgattgcca
     7081 aaggcttcgg cgttgataaa gttgcgggcg aactggaaaa atacggcatt caaaattatc
     7141 tggtcgaaat cggcggcgag ttgcacggca aaggcaaaaa tgcgcacggc gaaccgtggc
     7201 gcatcggtat agagcaaccc aatatcatcc aaggcggcaa tacgcagatt atcgtcccgc
     7261 tgaacaaccg ttcgcttgcc acttccggcg attaccgtat tttccacgtc gataaaaacg
     7321 gcaaacgcct ctcccacatc atcaatccca acaacaaacg acccatcagc cacaacctcg
     7381 cctccatcag cgtggtctca gacagtgcaa tgacggcgga cggtttatcc acaggattat
     7441 ttgttttagg cgaaaccgaa gccttaaggc tggcagaaca agaaaaactc gctgttttcc
     7501 taattgtccg ggataaggac ggctaccgca ccgccatgtc ttccgaattt gccaagctgc
     7561 tccgctaaac atacaaaccg attaggaaac accatgaaaa ccctgctcct caccttcggc
     7621 atcttcctga ccgtcatcat cggtatggcg gtcggctata ttttctccaa acgcaccatc
     7681 aaaggcagtt gcggcggcat taccgcttta ggtatgaaaa aaatgtgcga ctgcgacaca
     7741 ccttgcgaca ccctgcaaaa aaagctggat gaagaaaaac aggcaggcgg catacgggtt
     7801 gaccgttaac gccgaaacaa aatgccgcct gaagcccggc cggcgggttc agacggcatt
     7861 gccgctttac ttcgatcggt ttatacgcaa aacgcgcttc aggaaataaa tcagattatt
     7921 ccccagacgc cttcaggctt ccttctgtgc caccgtaacc atagccgggc gcaacacacg
     7981 gtcggacagg gtataaccct tcttcatcac accaaccacg gtattgggtt cctgctcact
     8041 ggccaccgcc tgcatcgcct gatggatatt cggatcgagc ttatcgcccg ctttaggatt
     8101 gatttccttg atttgcgtgg catcaaacgc cttctgcaac tcattcaaag tcatctgcac
     8161 gcccattttc agcgcatcga aattaccgct ctgatccaaa agcgccattt ccagataatc
     8221 cttgaccggc aacatttcca cggcaaactt ctgtccggcg aacttgtgcg tatcggcaat
     8281 ttcctgctgg tggcggcggc gcaggttttg ctcgtttgcc aaagcgcgca gttgttcgtc
     8341 tttcaactgc gcttccaact cggcaatccg cgcctgcaaa tcctcataag ccggctcggc
     8401 ggcagcctgt tcctgtacac cgtccgcatt tcctactgtc tcgacggttt ccaccgcctc
     8461 cacattttca accgcttctt cactgttttg ctgctgtgtc tgttcgctca tatcgtatcc
     8521 ctcaaaacaa attggaaatc aaaatccggt tattacccga gcaagataag gacattttca
     8581 agaaacttca agcaaaggca ggaaatttca catccgccgc cgaataccct accgcaaaaa
     8641 ccatataaac ggtaagatac cgccgacctt atgcttttca gacggcatcc attatgaaaa
     8701 aaaaccattt gaacacaacc ggtttcgacc tctggcacac catccgcgaa gaaaccgcgg
     8761 ccgctgccgc cgccgaaccg atgctggcaa gttttttgca ccaaaccgtg ttgcgccacg
     8821 agtccctcgg ctccgtcctt gcctaccacc tttccagcaa actcggcagc ccgattatgg
     8881 acgtgcgcgc gctgtttgaa atttaccagc aggcattggg cagcgacacc caaatcagca
     8941 aatgtgtcga agcagactta aaagccatct acgaacgcga tcccgcctgc gacgaatatt
     9001 cgctgccgct tttatatttc aaaggcttcc acgcgattca ggcacaccgc atcaaccacc
     9061 ggctgtatct cgacggacgc aaaacgctgg cgtatttcct acaaaaccgt atgtccgaag
     9121 tattcggcgt ggacatccat cccgccgccc gtttaggata cgggctgatg ctcgaccacg
     9181 ccaccggctt tgttgccgga gaaaccgccg tgttgggcaa caatatttcg attttgcacg
     9241 gcgtaacgct cggcggttcg ggcaaagaag gcggcgaccg ccaccccaaa atcggcgacg
     9301 gcgtgatgat cggcgcaaac gcctcgatat tgggcaatat ccgcatcggc agcaatgcca
     9361 aaatcggcgc gggcagcgtc gtggtttcag acgtgccgcc gtccatcacg gttgtcggcg
     9421 tacccgccaa acccgtggcg cgatcgctca aaaccccgtc ggcggatatg gatcaaaata
     9481 tccagtttgc cgaaatcgac tttatgattt gaatatccgc accaatgccg tctgaaacgc
     9541 caaacgggct tcagacggca ttgcccgctt caagcctgcg ccttaatatg ccgccgatgc
     9601 cggcacttat cagtcggaca aaatgccttt tattcgatac gcccgattcc cacgcaaccg
     9661 gaaataaccg gtttccttgt aaacgggcgg agtcgggcaa tgattcggac aaagcctatt
     9721 tcaaaagcaa aatcagcagc aaaacaaccg ctatcgctgc cagccacagg ctttgccgct
     9781 gctgcacttt gaccaaatgg atataggcat cgcgcatttc ctgccggcgc gcctcatcga
     9841 ccaaggcatt gattttgcgc ggcagggaag ggatgatttg cgcccagtct ggggcttcgt
     9901 ttttgaggtt gcgccaaagg gctttgggac cgacctgccc gttcatccat tttaccaaaa
     9961 acggtttggc ggttttccac aagtccaaat cgggatcgag ttggcggccc aagccttcga
    10021 tgttgagcag cgttttttgc agcaatacca gctgcggctg gatttcgaca ttgaagcggc
    10081 ggctgacttc aaacaggcgc atcagtacca agccgaagga aatctgcgaa atcggtttgt
    10141 tgaacacagg ctcgcacacg gcgcgaaccg ccgcttccaa ctcttccgca cgcgtgtcgg
    10201 caggcaccca gcccgattcg acgtgggcgg tggcgacacg tcggtaatcg cggttgaaaa
    10261 aggcgaggaa attgatggcg agatagcgtt tgtcgtaatc ggtcagcgta ccgacgatgc
    10321 cgaaatcaag ggcgatatag cggttgtcgg cggcaaccaa aatattgccg gggtgcatat
    10381 ccgcgtggaa aaagccgtcg cggaaaactt gcgtgaaaaa gatttccacg ccgtaatcgg
    10441 caagtttgtg caaatcgatg ccgtctgctt tgagtttggc gatgtcggaa accggcgtgc
    10501 cgtccatcca ttcaatcgtc agcacgtcgc ttgtgcagta gtcgtaaaac accttgggca
    10561 caatcagcat attgctgttt tggaaattgc gcccgagttg tcctgcattg gcggcttcgc
    10621 gcatcaaatc caattcgtcg tgcaggtatt tgtcgaactc cgccaccact tcgcgcggct
    10681 tcagacgctt gccgtctgaa aacaagcgtt cgacccaagc cgcgccgaaa cgcatcagcg
    10741 acaagtcttg ttcgataagg ggcaagaggt tggggcgcaa aactttgacc gccactcgtt
    10801 cgcccgaatg caggcgggct ttgtgtactt gggcgatgga cgcgctggcg acgggctcgg
    10861 tttcaaattc cgcatacagc ttttcgatgg gctgacccag cgatttttcg atttgttcac
    10921 gcgaaagccg cgcgtcaaaa ggcggcactt tgtcttgcag ctttgccagt tcgacggcgt
    10981 aatcgtgcgg aatcaaatcg gggcgtgtgg acaaaacctg cccgaacttg atgaaaatcg
    11041 gccccaggct ttccaaggca aggcgcagac ggacggcagg cggttcgttt ttcaatttgg
    11101 acgactgcgg catcattttc agcagagcgc atatccaacc gctacccatc agcgaaacgc
    11161 acagacctgc cagccgatag cggtaaaaag tcccgacaat gaccgtcagg cgtttcaacc
    11221 atttcatata taaaatcccg aatacagcat ttatcggaca atcaaaaaat cttaccgagc
    11281 agatagccca aaaaggcaat cagcaacccg aaaaccagcg gtttgatatt gcggggcggc
    11341 tcgggttgcg aagatgcgga aatatcatct tcccgcccgc cgtgaagttc aagatattcg
    11401 ggcatttccc catccgcgcc tcctccttcg ccctccccct cctccggcgg cacgaccgtt
    11461 tccaactcca tatacgggta ataatatcca aacccaaata gctgaaataa tcatgcaact
    11521 cttcccgttt cgcccaagtg catttttcct caaccagcac ttgcggtttg gaatcgagcg
    11581 attcgatcaa tccttccgcc ttttcttcgc cgagattctc cttaaggcac tcccggataa
    11641 gcccgcgatc cacttggggc gggtaggaaa catatacatc gtacaaatcg tttcggtaat
    11701 ctttactcat ctcaatcata agggaacaaa tacgcctgat accgttccgc ccgttccttt
    11761 cgggaaaccc gattcatctt caagcaatcg gattatatgt aaattcatcc cgataatata
    11821 ttttccaaaa gtaaaaatgc cgtccgaacg ttcagacggc ataacacaaa ccagccggtc
    11881 acacggaaaa cgacgatccg caaccgcagg ttgtttccgc gttcggattg cggatgacga
    11941 actgcgaacc ctgcagactt tccgtatagt cgatttccgc accgaccaga tattgatagc
    12001 tcatcggatc gaccaaaaag accaaaccgt ttttctcaat ttcaaaatcg tcgtcgtttt
    12061 tgatttcgtc aaaagtaaat ccgtactgga aacccgaaca gccgccgccg ttgacaaaaa
    12121 cccgcaattt caaatcggga ttgttttctt cggcaatcaa atcggcaact ttggcacagc
    12181 agctgtcagt aaaaataata gggctttcgt ccgacatagt gttattcctt aatataatat
    12241 tttctgcatt ctctctcaac cccccttcaa aggcaagcag ggcttcgcct ttgaaaaagg
    12301 aattgtccca atagataagt ttgaccgcct gcaatttcaa gccccaaact caaaccgtcc
    12361 tgccgaacca ctcgacacgc ccgataatct ccacatcatc ggtcaagtcg ttcaaattga
    12421 tttcaaaagc aggataagcc tcgttggcgg aaatgacatt gataatcccg ccggggacga
    12481 tttgcaggcg tttgaccaac agattttcat taatccgcaa cacatacagt ccgtctctcg
    12541 gcgtattttc cccatgattg accaaaatcg aatctccgtc attcaaaacc ccctccatcg
    12601 aatccccctt gacggaaatt acagacaggt ttttcgtatc tcgggtaaca taattctcaa
    12661 tccaatgccg tctgaacgcc attgtaaata ccggttcctc atgaccgaca aactgcccgt
    12721 atcccgcagc cgcccgaata tcatatctcg gcacaaagac aaactcgtcc gtatcaactt
    12781 catttcccaa ggtatcgtaa gcaagggatt tttttggggc ttcatccgga aacggattac
    12841 cctcccctgt cagcagccaa tcgatactac accccttcaa ctgcttaatt tttttcaatg
    12901 tttcagactt cggcagaccg ccttcattcc atattctgct gaagcccgca atcgtcattt
    12961 caatatcgga tgcgattttt gcctgcctcg cttcactttt ccataaaaaa accagtctgt
    13021 ctttaaaggt ttccataaat atccttccgc tccccttcat tcaatcatct aaagtaaaca
    13081 tgaaatatat tttacattag ctatcccaaa aacggaataa ctatgataaa ctaaatttaa
    13141 attatgattt ttcttatcca atacataatt tttcagattt tccataggtt tgatcccgaa
    13201 ttgacagaaa aaggatagaa aaatgaagaa aaccagcaaa tatcttatct atactgcggc
    13261 atttacctca ttctgctttg ccttccaaga aaaccgttct gaagccaaac agcccgacat
    13321 cactttatcc gcatccctgt gcgaacaatt caacatgctg aacgccaaag atatggatgc
    13381 agaacaagtc tccctttcca aagaatgcga catcatcgag tcttcacacg actgggaaaa
    13441 agagtacggc aacttgaacg aacaggaaat gctcgccggc gtcgtctatg aataaacctg
    13501 cctgccattt gaaacattat gcttgaatgc attggagcca aatgtattaa atcaaatata
    13561 aaaccaatat attcataaag ttatatactt atagccatgc tgtagcttga aacagcccgc
    13621 cgcccgccct atttacagcc catcgggaca aaatgttcca acatatttct caaaataagc
    13681 aaaatcaaat aggagtatcc acatggcaaa agtaatcggt atcgacttag gtacaaccaa
    13741 ctcttgtttg gccatttccg aaaacggtca aaccaaagtc atcgaaaacg cagaaggcgc
    13801 acgcaccacg ccgtccatta tcgcttattt ggacggcggc gaaatcctcg tcggcgcgcc
    13861 tgccaaacgc caagcggtaa ccaacgccaa aaacactatt tacgccgcca aacgtttgat
    13921 cggtcacaaa tttgaagaca aagaagtcca acgcgacatc gaatctatgc ctttcgaaat
    13981 catcaaagcc gacaacggcg acgcatgggt aaaagcacaa ggcaaagagc tgtctcctcc
    14041 tcaaatttcc gcagaagtcc tgcgtaaaat gaaagaagcc gccgaagctt acttgggcga
    14101 aaaagtaacc gaagccgtga ttaccgtccc tgcctacttc aacgacagcc aacgtcaagc
    14161 caccaaagac gcaggccgta tcgccggttt ggacgtaaaa cgcatcatca acgagccgac
    14221 cgcagccgct ttggcattcg gtatggacaa aggcgacaac aaagaccgca aaatagccgt
    14281 atatgacttg ggcggcggta ccttcgatat ttccatcatc gaaatcgcca acctcgacgg
    14341 cgacaaacaa tttgaagtat tggctaccaa cggcgatact ttcttgggcg gtgaagactt
    14401 cgaccaacgc ttgattgact acatcattga cgagtttaaa aaagaacaag gcattgattt
    14461 gaaacaagac gtaatggctc tgcaacgtct gaaagaagct gccgaaaaag ccaaaatcga
    14521 attgtccagc ggccagcaaa ccgaaatcaa cctgccgtac attaccatgg acgcaaccgg
    14581 cccgaaacac ttggcaatga aaattacccg cgccaaattc gagagcctgg ttgaagacct
    14641 gattgcccgc tctatcgagc cttgccgcac cgcattgaaa gatgccggct tgagcaccgg
    14701 cgacatcgac gacgtgattt tggtcggcgg tcagtcccgt atgccgaaag tacaagaagc
    14761 cgttaaagac ttcttcggca aagaaccgcg caaagacgtg aaccctgacg aagccgttgc
    14821 cgtaggtgca gcgattcaag gcgaagtatt gagcggcggc cgcagcgacg tattgctgct
    14881 ggacgtaacc cctctgtcct tgggtatcga aaccatgggt ggtgtgatga ccaagctgat
    14941 tcaaaagaac actactattc cgactaaagc gtctcaagtg ttctctactg ccgaagacaa
    15001 ccaaagcgca gtaaccatcc acgtactgca aggcgaacgc gaacgcgctt ctgccaacaa
    15061 atctttaggt cgattcaact tgggcgacat cgcacccgca ccacgcggta tgccgcaaat
    15121 tgaagttact ttcgacatcg acgccaatgg tatcttgcac gtttccgcca aagacaaagg
    15181 cactggcaaa gcggccaaca ttaccatcca aggttcttca ggtttgagcg aagaagaaat
    15241 cgaacgcatg gtgaaagatg ccgaagccaa tgccgaggaa gataaaaagc tgactgaatt
    15301 ggtcgcttcc cgcaaccaag ccgaagccct gattcactcc gtgaaaaaat ctttggcgga
    15361 ctacggcgac aaactcgacg ctgccgagaa agaaaaaatc gaagccgcgc tgaaagaagc
    15421 cgaagaagcc gtgaaaggcg acgacaaaac cgccatcgat gccaaagccg aagcactggg
    15481 tacagccagc caaaaactgg gcgaaatggt ttacgcgcaa gcgcaagccg aagcccaagc
    15541 cggcgagggc gcacaagcca atgcttctgc aaagaaagac gatgatgtcg tagatgcc
//
LOCUS       DAWTQS010000052        14916 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.052, whole genome shotgun sequence.
ACCESSION   DAWTQS010000052 DAWTQS010000000
VERSION     DAWTQS010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14916
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.052"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            391..1914
                     /locus_tag="WNA25_001564"
     CDS             391..1914
                     /locus_tag="WNA25_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HFA9824287.1"
                     /translation="MYRVCRLCAALCVWGAVGAYAAGLPDVRDDAAALRAQRAAAEGW
                     AGMPPEGDSAANGGSRVIDGDFLLSRPQLLEHVLRDALNGNQADLIASLADLYAKLPD
                     YDAVLYGRARALLAKLAGRPAEAVARYRALHRDNAADERVLLDLAAAEFDDFRLKSAG
                     LHFAEAAKLDLPAPVLENVGRFRKKTEGLTGWHFSGGISPSVNKNANNAAPQYCLQGG
                     GSRICSVTGPVRAAGLDYEAGAEKLTALADNHYLLFRANVGGTSYYFSRKSAYDDAFG
                     RAYLGWQYKNARQTVGVLPFYQAQLSGSDGFDAKTKPPADRRLAPYMLAHGAGVQLAH
                     SYRLSRRSQLSVSLERYRQRYREQGRAERNDGWQDGLYVSLARRLGGSATVFGGWQFA
                     RFVPKRETVGGAVNNAAYRRNAVHIGWAQEWGGTGGLNSRIAASYAHRNYKGIAAFST
                     EAQRNREWNVSLALSHDKLSYKGIVPTLNYRFGKTESNVPYAKRRNSEVFVSADWRF"
     gene            complement(1975..3849)
                     /locus_tag="WNA25_001565"
     CDS             complement(1975..3849)
                     /locus_tag="WNA25_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase component I
                     family protein/aminotransferase class IV"
                     /protein_id="HFA9824288.1"
                     /translation="MPYFALFDDAVSRRAKLYQNHVESRFFRPEELDALDGALQSGWQ
                     KGLHSVLFADYEFGLPLMGMESERGGNIALHWFADCADTDAESWLARHSDGIPAGIST
                     PQSSVSETDYLDRIRQIHEAIRRGDTYQINYTTRLHLQAYGNPVSLYRRLRQPVPYAV
                     LSHLPDEAGQSAWTLCFSPELFLNIASDGTISTEPMKGTAPILGDGQDERRAAELQTD
                     PKNRAENVMIVDLLRNDLGKIAQTGKVCVPEPFKVSRFGSVWQMTSTIRAQALPDTSF
                     ADTLRAAFPCGSITGAPKKMSMQIIESLETEARGLYTGSIGYLNPCSGGLGFEGAFNV
                     VIRTLSLKPVSDGIVSGIGGTDSNAQARTAGQGGETPHPFEANPPYRGVYGVGSGIVI
                     DSDPAAEYRECGWKARFLNELRPDFGIFETLRAENRQCALLDRHLCRLKTSAQALNLP
                     LPDGCENQIKQYIADLPDGAFRIKALLASDGISLSHAVLNRLTDKQRVIISPTILPAQ
                     NYLRRFKTTHRAIFDQAWQTAETQGAFDSLFFNSDGILLEGGRSNVFVKHRGQWLTPS
                     LDLDILNGIMRQAVSDEPQKYLHTNQVIETHITQKTLQGAEEIRLSNALRGVFAAVLA
                     "
     gene            4020..4949
                     /gene="ppk2"
                     /locus_tag="WNA25_001566"
     CDS             4020..4949
                     /gene="ppk2"
                     /locus_tag="WNA25_001566"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HFA9824289.1"
                     /translation="MTDRQLQPFENVELGEKQDQLQVFEKAVLEHEGKGSAEDSGTAP
                     LPENYPCRKRMRRAVYEAEKAKLQIELLKVQSRVKDSGQRIVSLFEGRDAAGKGGTIK
                     RFMEHLNPRGARVVALEKPTTTERGQWYFQRYIQNLPTAGEMVFFDRSWYNRAGVERV
                     TGFCEPNEYMLFMCQAPELERMLVASGIHLFKFWFSVSREEQLRRFISRRDDALKHWK
                     LSPVDIQSLDRWDDYTEAKNAMFFHTHTGDAPWVIIRSDDKKRARLNCIRYFLHQLDY
                     PGKDVKAIGKVDDKIVLVPDTRYKEKTIDVGHD"
     gene            complement(5031..6335)
                     /gene="aldA"
                     /locus_tag="WNA25_001567"
     CDS             complement(5031..6335)
                     /gene="aldA"
                     /locus_tag="WNA25_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFA9824290.1"
                     /translation="MTAARAAQPAWERLPAVERGAYLRKIAQGIRGRADELTDTIVAE
                     GGKTKDLARVEVMFTADYLDYQAEWARRYEGEIIQSDRPRENILSFKRPLGVIAGILP
                     RNFPFFLIARKMGPDLVTGNTIVVKPSSVTPINCHIFAEIIDAVGLPAGVFNVVDGPG
                     AEIGNALSAHPQVDMVSLTGSVEAGRQVMEAASANITKVSLELGGKAPAIVLKDADLD
                     LAVKSILASRVGNTGQICNCAERVYVHSSLKDAFIEKMTAAMKGVRYGNPAEAEAGAP
                     EMGTLIEERAVKAVAEKVERAVKQGAKLVCGGKRTEGRGYFFEPTLLTDTDNSMDIMK
                     EETFGLVLPVSAFDTLDQVIALANDCEFGLTSSVYTTNLNEAFYVTRRLQFGETYINR
                     ENFEAMQGFHAGWKKSGIGGADGKHGLEEYLQTQVIYLETDI"
     gene            complement(6262..6462)
                     /locus_tag="WNA25_001568"
     CDS             complement(6262..6462)
                     /locus_tag="WNA25_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFA9824291.1"
                     /translation="MKQLAMYINGRFENDFNGEWRDVLNPSTEETIAREPKGGRADVD
                     RGARGATGLGASACGRTRRVFA"
     gene            complement(6570..7418)
                     /locus_tag="WNA25_001569"
                     /pseudo
     CDS             complement(6570..7418)
                     /locus_tag="WNA25_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221212.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7658..8458
                     /locus_tag="WNA25_001570"
     CDS             7658..8458
                     /locus_tag="WNA25_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFA9824292.1"
                     /translation="MSPSPFIEMKDVAFAYGDRPILNDINFSIPQGNFAAVMGGSGSG
                     KTTLMRLITGQIRPQSGQVLIEGRDLAGFSADELYEHRRRMGVLFQHGALFTDLSVFD
                     NIAFPMRELTQLPEAVIRDLVLLKLNAVGLRGVENLMPSELSGGMSRRVALARTIALD
                     PEIMLYDEPFTGLDPISLGVIAHLISRVNKALRSTSIMVTHDIEKSLEIVDQVIFLAH
                     GEIMFSGSPQEMRELDSPWVRQFVGGLADGPVAYRYPAQTSLQQDLLG"
     gene            8506..9282
                     /gene="mlaE"
                     /locus_tag="WNA25_001571"
     CDS             8506..9282
                     /gene="mlaE"
                     /locus_tag="WNA25_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFA9824293.1"
                     /translation="MNFIRSVGAKTLGLIQPFGSITLFLLNILAKSGTAFARPRLSVR
                     QVYFAGVLSVLIVAVSGLFVGMVLGLQGYTQLSKFKSADILGYMVAASLLRELGPVLA
                     AILFASSAGGAMTSEIGLMKTTGQLEAMNVMAVNPVARVVAPRFWAGVFSMPLLASIF
                     NVAGIFGAYLVGVSWLGLDSGIFWPQMQNNITIHYDVINGLIKSAAFGVAVTLIAVHQ
                     GFHCIPTSEGILRASTRTVVSSALTILAVDFILTAWMFTD"
     gene            9333..9827
                     /gene="mlaD"
                     /locus_tag="WNA25_001572"
     CDS             9333..9827
                     /gene="mlaD"
                     /locus_tag="WNA25_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFA9824294.1"
                     /translation="MKKNILEFWVGLFVLIGAAAVAFLAFRVAGGAAFGGSDKTYAVY
                     ADFGDIGGLKVNAPVKSAGVLVGRVGAIGLDPKSYQARVRLDLDGKYQFSSDVSAQIL
                     TSGLLGEQYIGLQQGGDTENLAAGDTISVTSSAMVLENLIGKFMTSFAEKNAEGGNAE
                     KAAE"
     gene            9858..10448
                     /locus_tag="WNA25_001573"
     CDS             9858..10448
                     /locus_tag="WNA25_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFA9824295.1"
                     /translation="MKKSSFISALGIGILSIGMAFASPADAVGQIRQNATQVLTILKS
                     GDAASARPKAEAYAVPYFDFQRMTALAVGNPWRTASDAQKQALAKEFQTLLIRTYSGT
                     MLKFKNATVNVKDNPIVNKGGKEIVVRAEVGIPGQKPVNMDFTTYQSGGKYRTYNVAI
                     EGASLVTVYRNQFGEIIKAKGIDGLIAELKAKNGGK"
     gene            10499..10777
                     /locus_tag="WNA25_001574"
     CDS             10499..10777
                     /locus_tag="WNA25_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFA9824296.1"
                     /translation="MHTELKNGTLHIGGDITVKTLTGDAFGRFRQQCRLKETIAVDFG
                     GVKRADSACVSLLLEVLRGCKGSVRLTGIPESVRALSELYEIKDWLKS"
     gene            10774..11601
                     /locus_tag="WNA25_001575"
     CDS             10774..11601
                     /locus_tag="WNA25_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HFA9824297.1"
                     /translation="MKKTAYAILLLIGFASAPAFAETRPADPYEGYNRAVSKFNDQAD
                     RYIFAPAARGYRKVTPKPVRAGVSNFFNNLRDVVSFGSNILRLDIKRASEDLVRVGIN
                     TTFGLGGLIDIAGAGGVPDNKNTLGDTFASWGWKNSNYFVLPVLGPSTVRDALGTGIT
                     SVYPPKNIVFHTPAGRWGTTAAAAVSTREGLLDLTDSLDEAAIDKYSYTRDLYMKVRA
                     RQTGATPAEGTEDNIDIDELVESAETGAAEPAVHEDSVSETQAEAAGEAETQPGTQP"
     gene            11726..12088
                     /locus_tag="WNA25_001576"
     CDS             11726..12088
                     /locus_tag="WNA25_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ"
                     /protein_id="HFA9824298.1"
                     /translation="MYEVNRSVFVLIPLEPFWNWLQTLPGNHLDGLTLEDIQADANSY
                     LVRPCETADEVWDEIEARFEDIFAAELADWCEDEREWPALDADIFNEWFDIQLSTVIT
                     DLEHEPLAREAFQPINLN"
     gene            12085..12471
                     /locus_tag="WNA25_001577"
     CDS             12085..12471
                     /locus_tag="WNA25_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFA9824299.1"
                     /translation="MMKLTVRNYHLDGYGHVNNARYLEFLEEARWAFFEKRGLMHELA
                     GLILIVARIDIRYSRPAVEGDVLQFSCRLKTPGMRRIVLTQTITLPNGKTAAEADITL
                     MPVHAATQRTVSLPATLARALEALSE"
     gene            12468..12956
                     /locus_tag="WNA25_001578"
     CDS             12468..12956
                     /locus_tag="WNA25_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HFA9824300.1"
                     /translation="MKKILTAAAVALIGILLATVLIPDSKTAPAFSLPDLHGKTVSNA
                     DLQGKVTLINFWFPSCPGCVSEMPKVIKTANDYKNKDFQVLAVAQPIDPIESVRQYVK
                     DYGLPFTVIYDADKAVGQAFGTQVYPTSVLIGKKGEILKTYVGEPDFGKLYQEIDTAL
                     AQ"
     gene            complement(13037..13204)
                     /locus_tag="WNA25_001579"
     CDS             complement(13037..13204)
                     /locus_tag="WNA25_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFA9824301.1"
                     /translation="MEDDVWPGRRVIVMAGVTVGRGSVVGAGAVITKDIPPYSLAAGN
                     PAVVKKNLPEG"
     gene            complement(13171..13533)
                     /locus_tag="WNA25_001580"
     CDS             complement(13171..13533)
                     /locus_tag="WNA25_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-o"
                     /protein_id="HFA9824302.1"
                     /translation="MESKFFFLLLRFAGSVLPPSHMRGIGIIGRRVLGFLARRISPHI
                     GRGAYVFPDTVLGDGSGIGANCEICRGLVVGKNVMMGPECLFYSTNHKFDREKNALRA
                     TRKSVRLRWRTMSGRGAG"
     gene            13668..13883
                     /gene="rpmE"
                     /locus_tag="WNA25_001581"
     CDS             13668..13883
                     /gene="rpmE"
                     /locus_tag="WNA25_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFA9824303.1"
                     /translation="MKQGIHPNYREVNVTCSCGNKFVTKSAMEKENFNIEVCSLCHPF
                     YTGTQKIVDTTGRVDKFNNKFGNLFKR"
     gene            14141..14776
                     /locus_tag="WNA25_001582"
     CDS             14141..14776
                     /locus_tag="WNA25_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HFA9824304.1"
                     /translation="MFYDSKCCYFNNPVFWDSRRLTYYPNVIFCQKKSRKDIINIYLG
                     QFLGSVSLILLSLLFAFVLDYIPSKEILGLLGLIPIFLGLKVLLLGDSDGESIAKEGL
                     RKDNKNLIFLVAMITFASCGADNIGVFVPYFTTLNLANLIVALLTFLVMIYLLVFSAQ
                     KLAQVPSVGETLEKYSRWFVAVVYLGLGIYILIENNSFDMLWTVSGQEKIL"
BASE COUNT         3256 a         4044 c         4158 g         3458 t
ORIGIN      
        1 cacaggcggc aagggcggcg gcaagcggca gtaagggcag ggctttgtat ttcatcgcat
       61 actcaatctt gatgatgtat taataatagt tttgattatc aatttaaatg ttatatgatg
      121 tcaactgttt tatggggcgg cgcggaaatc ggacgtgcct gcgtttgcct gccgcgtttc
      181 tacctttgtt ttttatagcg gattaacaaa aaccggtacg gcgttgcctc gccttgccgt
      241 actatctgta ctgtctgcgg cttcgtcgcc ttgtcctgat ttttgttaat ccactatatg
      301 atgcgccgcg tccgccgcgc gtggggcttg cggcgtttct gccgccgccg tccggcggtg
      361 ttttttaagg aaggcaaatt gaaggatagt gtgtatcggg tatgccggct gtgtgccgcg
      421 ctgtgtgttt ggggtgcggt cggcgcgtat gccgccggct tgcccgacgt tcgggacgat
      481 gcggcggcgt tgcgcgccca gcgtgcggct gcggagggtt gggcgggaat gccgccggaa
      541 ggggattctg ccgcaaacgg cggttcgcgc gtaatcgacg gggatttcct gctgtcgcgc
      601 ccgcagttgt tggaacacgt tttgcgcgac gcgctcaacg gcaatcaggc ggatttgatc
      661 gcttcgttgg cggatttgta tgccaagctg ccggattatg atgcggtgtt gtacggcagg
      721 gcgcgggctt tgctggcgaa attggcggga aggccggcgg aggcggtggc gcgctatcgg
      781 gcgttgcaca gggataatgc ggcagacgag cgggttttgc tggatttggc ggcggcggag
      841 tttgacgatt tccggctgaa gtcggcaggg ctgcattttg cggaggcggc aaaattggat
      901 ttgccggcgc cggttttgga aaatgtgggg cgtttccgga aaaaaacgga ggggctgacg
      961 ggctggcatt tttcgggcgg catcagccct tctgtgaaca aaaacgccaa caatgccgcg
     1021 ccgcaatact gcctgcaggg cggcggcagc cggatatgca gcgttaccgg acctgtccgt
     1081 gcggcagggt tggattatga ggccggggcg gaaaagctga ctgcgctggc ggacaaccat
     1141 tatctgctgt tccgcgccaa tgtcgggggc acaagctatt atttcagccg caaatcggct
     1201 tatgacgatg ctttcggcag ggcttatttg ggctggcagt ataaaaatgc gcggcagacg
     1261 gtgggggttt tgccgtttta tcaggcgcaa ttgtcgggta gcgacggctt tgatgcgaaa
     1321 acgaaaccgc ctgccgaccg ccggcttgcg ccgtatatgc tggcgcacgg ggcgggtgtg
     1381 cagttggcgc attcttaccg cctgagccgc cggtcgcagc tttcggtgtc gctggaacgt
     1441 taccgccaac gctaccgcga acaaggccgg gcggaacgca acgacggttg gcaggacggg
     1501 ctttatgtgt cgctggcaag gcggttgggc ggctcggcaa ccgtgttcgg cggctggcag
     1561 tttgcgcggt ttgtgccgaa acgcgaaacg gtgggcggcg cggtcaacaa tgccgcctac
     1621 cgccgcaacg ccgtccatat cggttgggcg caggagtggg gcgggacggg cggtttgaac
     1681 agccggattg ccgcgtctta cgcccaccgc aactacaaag gcattgcggc attttcgacg
     1741 gaggcgcagc gtaaccgcga atggaatgtc tcgctggctt tgagccacga caagttgtcg
     1801 tacaaaggca tcgtgccgac gttgaactac cgcttcggta agacggaaag caatgtgccg
     1861 tatgcaaaac gccgcaacag cgaggtgttt gtgtcggcgg attggcggtt ttgaatggtg
     1921 ggataatgcc gtccgaactt tgcggcagca ggttcggacg gcatttttgc gcgttcaggc
     1981 aaggacggcg gcaaatacgc cgcgcaaggc gttggagagg cggatttctt cggctccttg
     2041 cagtgttttt tgtgtgatgt gtgtttcaat cacttgattt gtgtgcaaat atttttgcgg
     2101 ttcgtccgac acggcttggc gcattatgcc gtttaaaatg tctaaatcta aagagggcgt
     2161 gagccattgt ccgcgatgtt tgacgaacac gttgctcctg ccgccttcga gtaggatgcc
     2221 gtccgaattg aaaaacaggc tgtcgaacgc gccttgcgtt tcggcggttt gccacgcttg
     2281 gtcgaagatg gcgcggtgtg tggttttgaa gcggcgcagg tagttttgtg cgggcaggat
     2341 ggtcggcgaa atgatgacgc gctgtttgtc ggtcagacgg tttaaaacgg cgtgggacag
     2401 gctgatgccg tctgaagcga gcagggcttt gatgcggaac gcgccgtcgg gcaagtcggc
     2461 aatgtattgt ttgatttgat tttcgcagcc gtcgggcagg ggcaggttga gggcttgggc
     2521 ggaggttttc agacggcata ggtggcggtc gagcagggcg cattgcctgt tttccgcgcg
     2581 cagggtttca aaaatgccga aatcggggcg cagttcgttg aggaatcggg ctttccagcc
     2641 gcattcgcga tattcggcgg cggggtcgct gtcgatgacg atgccggaac cgacaccgta
     2701 cacgccccga tacggcggat tggcttcaaa cggatgcggc gtttccccgc cttgtcctgc
     2761 cgttcgggct tgcgcgtttg aatccgtgcc gccgatgcct gaaacaatgc cgtctgaaac
     2821 gggtttgagc gacagggtgc ggatgacgac gttgaacgcg ccttcaaacc ccaagccgcc
     2881 ggaacacggg ttcaaatagc cgatgctgcc cgtataaagc ccgcgcgctt cggtttcgag
     2941 cgattcgata atctgcatac tcattttttt gggcgcgccg gtgatgctgc cgcaggggaa
     3001 ggcggcgcgg agggtgtcgg cgaacgaggt gtccggcagg gcttgcgccc ggatggtgct
     3061 ggtcatctgc cagacgctgc cgaaacgcga tactttaaac ggctcgggta cgcatacttt
     3121 accggtttgg gcgattttgc cgaggtcgtt gcgcagcaaa tcgacaatca tcacgttttc
     3181 ggcgcggttt ttcgggtcgg tttgtaactc ggcggcgcgg cgttcgtctt gtccgtcgcc
     3241 caaaatcggc gcggtgcctt tcatcggttc ggtgctgatg gtgccgtctg aagcgatgtt
     3301 gaggaagagt tcgggcgaga aacacagcgt ccacgcggat tgcccggctt catcgggcag
     3361 gtgggacaat acggcatagg ggacgggctg gcgcaggcgg cggtagaggc tgacggggtt
     3421 gccgtaggct tgcaggtgca ggcgggtggt gtagttgatt tgataggtgt cgccgcgccg
     3481 gatggcttcg tggatttggc ggatgcggtc gaggtaatcg gtttcggata cggaggattg
     3541 cggcgtggaa ataccggcgg ggatgccgtc tgaatgtcgg gcaagccagc tttcggcatc
     3601 ggtgtcggcg cagtcggcaa accagtgcag ggcaatgttg ccgccgcgtt cggattccat
     3661 ccccatcagc ggcaaaccga attcgtagtc tgcaaacaac accgaatgca gccctttttg
     3721 ccatcccgat tgcagcgcgc cgtctaaagc atcgagttct tcgggacgga aaaaacggct
     3781 ttccacatga ttttgataga gttttgcgcg tcggctgacg gcatcgtcaa acagggcgaa
     3841 ataaggcatg gcaattcggg gcaaatgttt tgattatacg ccccttttac acggattttc
     3901 agacggcggg gcaaattcgg gcaacaaaat gtaattttat gtagagaaag cggtggcgaa
     3961 agtgtaaaat tgtgaaaacg gactttcatt tccaacctga aatacaatag gagaccttta
     4021 tgacagaccg ccagttgcaa ccgtttgaaa acgtagaatt gggcgaaaag caagaccagc
     4081 tccaagtatt tgaaaaagct gttttggaac atgaaggcaa aggttctgcc gaagattccg
     4141 gcacagcccc gctgcccgaa aactacccct gccgcaaacg tatgcgccgt gccgtatacg
     4201 aagccgaaaa agccaaactg caaatcgagc tgctgaaagt gcaaagccgg gtcaaagact
     4261 ccggccagcg catcgtcagc ctgtttgaag gccgggatgc ggcaggtaag ggcggcacca
     4321 tcaaacgctt tatggaacat ttaaacccgc gtggcgcgcg cgtggttgct ttggagaaac
     4381 caaccactac cgaacgcgga caatggtatt tccaacgcta catccaaaac ctgccgactg
     4441 cgggcgaaat ggtattcttc gaccgctcgt ggtacaaccg cgccggcgtg gaacgcgtga
     4501 cgggcttctg cgaacccaac gaatatatgc tctttatgtg ccaagcccct gagttggaac
     4561 gtatgcttgt cgccagcggc atccacctgt tcaaattctg gttctccgta tcccgcgaag
     4621 aacaactgcg ccgcttcatc tcccgccgcg acgacgccct gaaacactgg aaactttccc
     4681 ccgtggacat ccagtcgctc gaccgctggg acgactacac cgaagccaaa aacgcgatgt
     4741 tcttccacac ccacaccggc gacgcgcctt gggtcatcat ccgttcggac gacaaaaaac
     4801 gcgcccgttt gaactgtatc cgctacttcc tgcaccagtt ggactacccg ggtaaagacg
     4861 tgaaagccat cggcaaagtg gacgataaaa tcgttcttgt tcccgatacg cgttacaaag
     4921 agaaaaccat cgatgtcggt catgactgat tgccggtgaa gtaataaaaa tgccgtctga
     4981 acccgatttc aggttcagac ggcattttct atccgtgtgt caaaccggca tcagatgtcg
     5041 gtttccagat aaatgacttg ggtttgcagg tattcttcca aaccgtgttt gccgtccgcg
     5101 ccgccgatgc cggatttttt ccagcccgcg tggaagccct gcatggcttc gaagttttcg
     5161 cggttgatgt aggtttcgcc gaattgcagg cggcgggtaa cgtagaaggc ttcgttcagg
     5221 ttggtggtgt acacggagct ggtcagaccg aattcgcagt cgtttgccag ggcgatgacc
     5281 tggtcgagcg tgtcgaaagc ggaaacgggc agcacgaggc cgaaagtttc ttctttcata
     5341 atgtccatac tgttgtcggt gtcggtcagc agggtaggct cgaagaaata gccgcgtcct
     5401 tcggtgcgtt tgccgccgca aaccaatttc gcaccttgtt tgacggcccg ttccactttt
     5461 tcggcaacgg ctttgacggc gcgctcttca atcagcgtgc ccatttccgg cgcgcctgct
     5521 tcggcttcgg cagggttgcc gtagcgcacg cctttcatcg cggcggtcat tttttcaatg
     5581 aacgcgtctt tcaggctgct gtggacatag acgcgctcgg cgcagttgca gatttgaccg
     5641 gtgttgccga cgcgcgaagc caagatggat ttcaccgcta aatccaaatc cgcgtctttc
     5701 aacacgatgg cgggggcttt gccgccgagt tccagcgaga ctttggtgat gttggcggaa
     5761 gcggcttcca tcacttggcg acccgcttca accgagccgg tcaggctgac catatcgact
     5821 tgcggatggg cggacaaggc gttgccgatt tccgcacccg gaccgtccac cacgttgaac
     5881 acgcctgcgg gcagtccgac cgcatcgatg atttcggcga agatgtggca gttgatcggg
     5941 gttacgctgc tgggtttgac gacgatggtg ttgcccgtaa ccagatccgg gcccattttg
     6001 cgggcgatca ggaagaaggg gaagttccgc ggcaaaatgc cggcaatcac gcccagcgga
     6061 cgtttgaacg ataaaatgtt ttcgcgcggg cggtcgcttt ggatgatttc gccttcgtaa
     6121 cggcgcgccc attcggcttg gtaatcgaga tagtcggcag tgaacatcac ttccacacgc
     6181 gccaagtctt tggttttgcc gccttcggca acgatggtgt cggtcagctc gtcggcgcgt
     6241 ccgcgtatgc cttgggcgat tttacgcaaa tacgcgccgc gttcgaccgc aggcagacgc
     6301 tcccaagccg gttgcgccgc gcgcgccgcg gtcaacgtcc gccctgccgc ctttgggttc
     6361 gcgggcgatg gtttcttcgg tggacgggtt caatacgtcg cgccattcgc cgttgaaatc
     6421 gttttcaaag cgtccgttga tgtacatggc caattgtttc atttcgggcc ctccagtttt
     6481 gtagtcggat gtagttttgg tttattccca aataaattgc cgtacaagtt tctttacacg
     6541 cggattttgg gtttcaagtc aaatacggct taccggtttt cttttttagt ttccgcaggc
     6601 gtgctgccga aatattgcct gaatgcctga ataaagctgg aaacgtggcg ctagccgcac
     6661 aaatacgcgg tttcgcccac gtttttgccc ccgttttgca acagatagag cgcgtgctgc
     6721 atttgtttgt ggtgcagcca ctcgcttgcc gtaatgccga aatggtcgcg catacggcgt
     6781 tgcagcgtcc tttcgctgat gttcagcgcg gctgtcagcc ggttgacttg gtgtgcgccg
     6841 ccgtcgaacg cggcattcag ggtgcggctg aagtcttcag acggcatagc gtctgcttcc
     6901 gccgtttgcc ccgccgccgg ctcgatgccg tctgaaaccg tgtcccacaa atccgacagc
     6961 agccgcaaca cgtccgcctc gcggcgcaat gtttcgccca aatgcccctt tgggacggtt
     7021 tgcaggcagg atgccgccaa gccgcgcagg tttgggggca aatcccatat cctgaccggt
     7081 tcgcggtaaa gcaggggtgc gaaacgcgcg tattccggac gcggcagcca ttgttccata
     7141 cctttaatgg tcattttgac cgttttgccg ccgcgataaa gatagcggct gaacaggact
     7201 tcttccccga cggcaattag gacaatcttg ccgccgtccg catcgatgcg gaagcggcgg
     7261 cggttgatgc cgaagtccaa acgcccttcc agcaacagca cgaacgacac ataaggttcg
     7321 gcgaggcggc tgctgcaaaa atcgcaccgt gccgttaccg taccgccgtg gatggaaatg
     7381 ccgttggaca aagtatcgaa acggtagctg ccctccacat aagccgccgc cgcttttgac
     7441 cagattgacg aaccggctgc tgttcaaatg ttctgctttt tccatagttt tctgataaag
     7501 gttatcattt gaaagataac atttttcgcc acagcaaaca atccgccgcc gacaaaggca
     7561 ttttcacaac acttgccgtc cgtatgccgt ctgaaaattg cccgtcttcc cgataagcct
     7621 taaaatacac gccgtcaaac ctaccgtccg cccgtacatg agcccatccc cctttatcga
     7681 aatgaaagac gtcgccttcg cgtatggcga ccgcccgatt ctgaacgaca tcaatttcag
     7741 cattccgcaa ggcaattttg ccgccgtgat gggcggttcg ggcagcggca aaaccacgct
     7801 gatgaggctg attacaggac agattcgtcc gcagtccggg caggttttga ttgaaggacg
     7861 ggatttggcg ggtttttcgg ctgacgaact ctacgaacac cgccgccgta tgggcgtatt
     7921 gttccaacac ggtgcgctgt ttaccgattt gtcggtattc gacaatatcg cttttccgat
     7981 gcgcgaactg acgcaacttc cggaagcggt tattcgcgat ttggttttgt tgaaattgaa
     8041 cgcggtcggt ctgcgcggcg tggaaaacct gatgccgtcc gagttgtccg gcgggatgtc
     8101 gcgccgcgtc gcgctggcac gcacaatcgc gctcgacccc gaaattatgt tgtacgacga
     8161 accgtttacc ggcctcgacc cgatttcctt gggcgtgatt gcccacttga tcagccgcgt
     8221 caacaaggcc ttgcgttcga ccagcattat ggtaacgcac gacattgaaa aatctttgga
     8281 aatcgtcgat caggtaattt tcttggcgca cggcgaaatt atgttctccg gctcgccgca
     8341 ggaaatgcgc gaactggatt cgccttgggt gcgccagttt gtcggcgggc tggcagacgg
     8401 ccccgtggca taccgctatc cggcgcaaac gtcgttgcag caggatttgc tcgggtaaat
     8461 gagcaggtgc cgtctgaaac cgttgaacct tacatataga aatttatgaa ttttatccgt
     8521 tccgtcgggg cgaaaaccct cggccttatt caacccttcg gcagtatcac gctgtttctg
     8581 ctgaacattt tggcgaaatc cggcacggct ttcgcccgtc cgcgcctgag cgtgcgccaa
     8641 gtgtattttg ccggcgtgct gtcggtgctg attgttgccg tttcggggct gttcgtcggt
     8701 atggttttgg gtttgcaggg ctatacgcag ttgtcgaaat tcaaatccgc cgatattttg
     8761 ggctatatgg tcgcggcttc tctgttgcgc gaactgggtc ccgtgttggc ggcgattctg
     8821 tttgccagca gcgcgggcgg tgcgatgacc agcgaaatcg gtttgatgaa aacgaccgga
     8881 cagctcgaag cgatgaacgt gatggcggtc aaccccgtcg cccgcgtggt tgccccgcgt
     8941 ttttgggcgg gcgtgttttc tatgccgctt ttggcttcga ttttcaacgt cgcgggcatt
     9001 ttcggcgcgt atttggtcgg cgtgagctgg ctgggtttgg acagcggtat tttctggccg
     9061 cagatgcaga acaacattac gatacattac gatgtaatca acggtttgat caaatccgcc
     9121 gcgttcggcg tggcggtaac gctgattgcc gtgcatcagg gcttccactg catcccgact
     9181 tcggaaggca ttttgcgcgc cagcacgcgc acggtggttt cgtccgccct gacgattttg
     9241 gcggtcgatt ttatattgac cgcgtggatg tttacagatt gacagactgt ctgaagacga
     9301 aacgcggaac atcggatatt caaggaactt taatgaaaaa gaacatattg gaattttggg
     9361 tcggactgtt cgtcctgatc ggcgcggcgg cggttgcctt tctcgctttc cgcgtggcgg
     9421 gcggcgcggc gttcggcggt tcggacaaaa cttacgccgt ttatgccgat ttcggcgaca
     9481 tcggcggttt gaaggtcaat gcccccgtca aatccgcagg cgtattggtc gggcgcgtcg
     9541 gcgctatcgg gcttgacccg aaatcctatc aggcgagggt gcgccttgat ttggacggca
     9601 agtatcagtt cagcagcgac gtttccgcgc aaatcctgac ttcgggactt ttgggcgaac
     9661 agtacatcgg gctgcagcag ggcggcgata cggaaaacct tgctgccggc gacaccatct
     9721 ccgtaaccag ttctgcaatg gttctggaaa acctgatcgg taaattcatg accagcttcg
     9781 ccgagaaaaa cgctgagggc ggcaatgcgg aaaaagccgc agaataaaca tacacatcca
     9841 ttttcaggaa agacatcatg aaaaaatcct ccttcatcag cgcattgggc atcggtattt
     9901 tgagcatcgg catggcattt gcctccccgg ccgacgcagt gggacaaatc cgccaaaacg
     9961 ccacacaggt tttgaccatc ctcaaaagcg gcgacgcggc ttctgcacgc ccaaaagccg
    10021 aagcctatgc ggttccctat ttcgatttcc aacgtatgac cgcattggcg gtcggcaacc
    10081 cttggcgtac cgcgtccgac gcgcaaaaac aagcgttggc caaagaattt caaaccctgc
    10141 tgatccgcac ctattccggc acgatgctga aattcaaaaa cgcgaccgtc aacgtcaaag
    10201 acaatcccat cgtcaataag ggcggcaagg aaatcgtcgt ccgtgccgaa gtcggcatcc
    10261 ccggtcagaa gcccgtcaat atggacttta ccacctacca aagcggcggc aaataccgta
    10321 cctacaacgt cgccatcgaa ggcgcgagcc tggttaccgt gtaccgcaac caattcggcg
    10381 aaatcatcaa agccaaaggc atcgacgggc tgattgccga gttgaaagcc aaaaacggcg
    10441 gcaaataacc gacaaaccat accgtccgag ggcggtatgg gtttttcgaa caaacgctat
    10501 gcatacagaa ctcaaaaacg gaacactgca catcggcggc gacatcaccg tcaaaaccct
    10561 gaccggggac gcatttgggc gtttccggca acaatgccgt ctgaaagaaa ccattgccgt
    10621 cgatttcgga ggtgtcaaac gtgccgattc cgcctgcgtg tcgctgctgc tcgaagtgct
    10681 gcgcggctgc aaaggcagcg tcaggctgac cggcattccc gaatccgtgc gcgcgctgtc
    10741 cgaactgtac gaaatcaaag actggctgaa atcatgaaaa aaaccgccta cgccatcctc
    10801 ctgctgatcg ggttcgcttc cgcccctgca tttgcagaaa cccgccccgc cgacccttat
    10861 gaaggctaca accgcgccgt ttccaaattc aacgaccaag ccgaccgcta cattttcgcc
    10921 cctgccgcgc gcggctaccg caaagttacg ccgaaacccg tccgcgccgg cgtgtccaat
    10981 ttttttaaca acctgcgcga cgtggtcagt ttcggcagca atatcttgcg tttggacatc
    11041 aaacgcgcaa gcgaagacct cgtccgcgtc ggcatcaata ccaccttcgg tttgggcggg
    11101 ctcattgata ttgccggcgc gggcggcgtt cccgacaata aaaacacttt gggcgacacg
    11161 tttgcctcgt ggggctggaa aaacagcaat tatttcgtgt tgcccgtctt aggcccgtcc
    11221 accgtccgcg acgcgctcgg cacgggcatt acctctgttt atccgcccaa gaatatcgtt
    11281 ttccataccc ctgccggacg ctggggcacg actgccgctg ccgccgtcag tacgcgcgaa
    11341 ggcctgctcg atttgaccga cagtctggac gaagccgcca tcgacaaata cagctacacg
    11401 cgcgacctct atatgaaagt ccgcgcacgg cagaccggtg caacacctgc cgaaggtacg
    11461 gaagataaca tcgacatcga cgaattggtc gaaagtgccg aaaccggcgc ggcagagccc
    11521 gccgttcacg aagattccgt atccgaaaca caggcagaag cagcagggga agccgaaacg
    11581 caacctggaa cacaacccta aagccatgcc gtctgaaaat ccgtgttcgg acggcattcc
    11641 cttccgtcgg aacggacagt ccgacactac agattgggct ataatccctt ttttgcgcat
    11701 accgcgcgac agcaaaggaa aaaccatgta cgaagtcaac cgcagcgtat tcgtcctcat
    11761 ccctttagaa cctttttgga actggctgca aaccctgccg ggcaaccacc tcgacggact
    11821 gaccctcgaa gacatccaag ccgatgccaa ttcctacctt gtccgcccgt gcgaaaccgc
    11881 cgacgaagta tgggacgaaa tcgaagcccg tttcgaagac attttcgccg ccgaacttgc
    11941 cgactggtgc gaagacgaac gcgagtggcc cgcactcgat gccgacattt tcaacgaatg
    12001 gttcgacatc cagctttcta ccgtcatcac cgacctcgaa cacgaaccgc ttgcccgcga
    12061 agccttccaa cccatcaacc tgaactgatg aagctgaccg tccgcaatta ccatctcgac
    12121 ggctacggtc atgtcaacaa tgcacgctac ctcgaatttc tcgaggaagc gcgctgggcg
    12181 ttttttgaaa aacgcggact gatgcacgag ttggcaggcc tcatactgat tgtcgcccgc
    12241 atcgacatcc gatacagccg tcccgccgtc gaaggcgacg tattgcagtt ttcatgccgc
    12301 ctgaaaaccc ccggcatgcg ccgcattgtg ctgacccaga cgattacact gccaaacggc
    12361 aaaaccgccg cagaagccga catcaccctg atgcccgtcc acgccgccac acaacgcacc
    12421 gtcagcctgc ccgccaccct tgcccgcgca ctggaagcct tgtccgaatg aaaaaaatac
    12481 tcaccgccgc cgccgtcgca ctgatcggca tcctccttgc caccgtcctc atccccgaca
    12541 gtaaaaccgc gcccgccttc tccctgcccg acctgcacgg aaaaaccgtt tccaacgccg
    12601 acctgcaagg caaagtcacc ctgattaatt tttggtttcc ctcctgtccg ggttgtgtga
    12661 gcgaaatgcc caaagtcatc aaaacggcaa acgactacaa aaataaagat ttccaagtcc
    12721 tcgccgttgc ccagcccatc gatccgatag aaagcgtccg ccaatacgtc aaagactacg
    12781 gactgccgtt taccgtcatt tatgatgcgg acaaagccgt cggacaggca ttcggcacac
    12841 aggtttatcc gacttccgtc cttatcggca aaaaaggcga aattctcaaa acctacgtcg
    12901 gagaacccga tttcggcaaa ctctaccaag aaatcgatac cgcgctggcg caatagcgat
    12961 gccgtctgaa gcgcaaaaaa cagaaacgat gttcctgttt tttcagatca tccgccccga
    13021 cacgttcagg cggcattcaa ccttccggca gattcttttt taccaccgca ggattgcctg
    13081 ccgccaaaga gtagggcgga atgtcttttg taattaccgc gcctgcgccc acgaccgagc
    13141 cgcgtccgac ggttacgccc gccatcacaa tcaccctgcg ccccggccag acatcgtcct
    13201 ccaacgtaat cggacggatt tccgtgtagc cctcaaagcg tttttttcac ggtcaaactt
    13261 gtggttggtc gaatagaaca ggcattccgg ccccatcatc acatttttgc cgaccaccag
    13321 tccacggcag atttcacagt ttgccccgat gcccgagccg tcgcccaaaa ccgtatccgg
    13381 aaacacatac gccccgcgtc cgatatgcgg cgaaatccgc cgcgccagaa aaccgagcac
    13441 ccgtctgccg atgatgccga tgccgcgcat atgcgacggc ggcagcaccg aaccggcaaa
    13501 acgcagcagc aggaaaaaga attttgattc catcaaacct tcctcaaaag aactttttac
    13561 attttatcga atatcctctt gacgaaacaa cagaaaatag cgagaatgcc gagcttgtct
    13621 gaggcatagg tacatcccct atgcccgctt ttttgaaagg aaatcaaatg aaacaaggta
    13681 ttcacccgaa ctaccgcgaa gttaacgtta cctgctcttg cggcaacaaa ttcgtaacca
    13741 aatccgcaat ggaaaaagaa aacttcaaca tcgaggtttg ctccctgtgc cacccgttct
    13801 ataccggcac ccaaaaaatc gtcgatacca ccggccgcgt ggacaaattc aacaacaaat
    13861 tcggcaacct gttcaaacgc tgattgccgc tttgcaagaa ttaagccctg ccatcatttc
    13921 gatggcaggg ctttttaata tttggcacac tgacttctta tctatatagt ggattaacaa
    13981 aaatcaggac aaggcgacga agccgcagac agtacaaata gtacggcaag gcgaggcaac
    14041 gccgtaccgg tttttgttaa tccactatat tttgacgggg ttgaagttga cagctacaaa
    14101 gtttaatgtt tgaatacatt aaaaaatata tttgaatggg atgttttatg attcaaaatg
    14161 ttgttacttc aataatcctg tattctggga cagccgtaga cttacttatt atcctaatgt
    14221 tattttttgc caaaaaaaaa gcagaaaaga catcattaac atctatttag gacaatttct
    14281 aggctctgtt agcctgatat tgctaagttt gctttttgca tttgtcttag attatattcc
    14341 tagtaaagag attttaggtt tgctcggctt gattccgatt ttcctaggcc tcaaagtttt
    14401 gctgttagga gattccgatg gagagtctat tgccaaagag ggtttgcgca aagataataa
    14461 aaacctgatt tttctagtcg ctatgattac ttttgcaagt tgtggcgctg acaatattgg
    14521 tgtctttgtc ccatatttta ctaccttaaa tttagcgaat ttgatagtgg ctttacttac
    14581 ctttctagtt atgatttatc tcttggtttt ttctgcccaa aaattggcac aagtcccttc
    14641 tgtcggagaa actttggaaa aatatagcag atggtttgtt gccgttgttt atttaggatt
    14701 ggggatatat atcctgattg aaaacaacag ttttgatatg ctatggactg tgtcgggcca
    14761 ggaaaaaata ttatgaaaaa gatagtatct gcaaagactg ccccgtgcag tcttttttgt
    14821 tgccggtctt tttgtgtctg atgccgtctg aagcagtctc tgcacgacct ttgtgcgaat
    14881 atttgctaca cttggcaatc cgtccatttc ttctca
//
LOCUS       DAWTQS010000053        14607 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.053, whole genome shotgun sequence.
ACCESSION   DAWTQS010000053 DAWTQS010000000
VERSION     DAWTQS010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14607
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.053"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            565..1440
                     /gene="prpB"
                     /locus_tag="WNA25_001583"
     CDS             565..1440
                     /gene="prpB"
                     /locus_tag="WNA25_001583"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFA9824305.1"
                     /translation="MSQHSAGARFRQAVKESNPLAVVGCVNAYFARLATQSGFKAIYL
                     SGGGVAACSCGIPDLGITTMEDVLIDARRITDNVDTPLMVDIDVGWGGAFNIARTIRN
                     FERAGVAAVHIEDQVAQKRCGHRPNKAIVSKDEMVDRIKAAVDARVDENFVIMARTDA
                     LAVEGLDAAIERAQACVEAGADMIFPEAMTDLKMYRQFADAVKVPVLANITEFGATPL
                     YTQSELAENGVSLVLYPLSSFRAASKAALNVYEAIMRDGTQAAVVDSMQTRAELYEHL
                     NYHAFDQKLDKLFQK"
     gene            1525..2673
                     /gene="prpC"
                     /locus_tag="WNA25_001584"
     CDS             1525..2673
                     /gene="prpC"
                     /locus_tag="WNA25_001584"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFA9824306.1"
                     /translation="MTETTQTPTFKPKKSVALSGVAAGNTALCTVGRTGNDLSHRGYD
                     ILNLAQKCEFEEVAHLLIHGHLPNKFELAAYKTKLKSMRGLPIRVIKVLETLPAHTHP
                     MDVMRTGVSMLGCVHPERESHPDSEARDIADKLIASLGSILLYWYQYSHNGKRIEVES
                     DEETVGGHFLHLLHGKRLTESQVKAMHVSLILYAEHEFNASTFTARVIAGTGSDMYSC
                     ITGAIGALKGPKHGGANEVAYDIQKRYRNADEADIRERIGRKEIVIGFGHPVYTISDP
                     RNVVIKEVARGLSREAGDMRLFDIAECLESVMWEEKKMFPNLDWFSAVSYQKLGVPTA
                     MFTPLFVISRTTGWSAHVLEQRKDGKIIRPSANYTGPEDLAFVEIEER"
     gene            2825..3613
                     /locus_tag="WNA25_001585"
     CDS             2825..3613
                     /locus_tag="WNA25_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HFA9824307.1"
                     /translation="MEDLYIILALGLVAMIAGFIDAIAGGGGLITLPALLLAGIPPVS
                     AIATNKLQAAAATFSATVSFARKGLIDWKKSLPIAAASFAGGVVGALSVSLVSKDILL
                     AVVPVLLIFVALYFVFSPKLDGSKEGKARMSFFLFGLTVAPLLGFYDGVFGPGVGSFF
                     LIAFIVLLGCKLLNAMSYTKLANVACNLGSLSVFLLHGSIIFPIVATMAVGAFVGANL
                     GARFAVRFGSKLIKPLLIVISISMAVKLLIDERNPLYQMIVSMF"
     gene            3690..6295
                     /gene="acnD"
                     /locus_tag="WNA25_001586"
                     /pseudo
     CDS             3690..6295
                     /gene="acnD"
                     /locus_tag="WNA25_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
     gene            6845..8007
                     /gene="prpF"
                     /locus_tag="WNA25_001587"
                     /pseudo
     CDS             6845..8007
                     /gene="prpF"
                     /locus_tag="WNA25_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464330.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
     gene            8319..9518
                     /locus_tag="WNA25_001588"
     CDS             8319..9518
                     /locus_tag="WNA25_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFA9824308.1"
                     /translation="MSDQLILVLNCVSSSLKGAVIDRKSGSVVLSCLGERLTTPEAVI
                     TFNKDGNKRQVPLSGRNCHAGAVGMLLNELEKHGLHDRIKAIGRRIAHGGEKYHESVL
                     IDQDVLDELKACIPFAPLHNPANISGILAAQEHFPGLPNVGVMDTSFHQTMPERAYTY
                     AVPRELRKKYAFRRYGFHGTGMRYVAPEAARILGKPLEDIRMIIAHLGNGASITAVKN
                     GKSVDTGMGFTPIEGLVMGTRCGDTDPGVYSYPTFHAGMDVAQVDEMLNEKSGFPGIS
                     ELPNDCRTLEIAADEGREGARLALEVMTCRLAKYIASMAVACGSVDALVFTGGIGENS
                     RNIRAKTVSYLDFLGLHIDTKANMEKRYGNSGIISPTDSSPAVLVVPTNEELMIACDT
                     AELAGIL"
     gene            9629..10249
                     /locus_tag="WNA25_001589"
     CDS             9629..10249
                     /locus_tag="WNA25_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HFA9824309.1"
                     /translation="MAQFFAVHPDNPQERLIKQAVEIINQGGVVVYPTDSCYALGCKL
                     GDKAAMERILSIRKIDLKHHLTLMCADLSELGTYAKIDNVQFRQLKAATPGPYTFILQ
                     ATKDAPARALHPKRKTIGLRIPDNAVAQALLEELGGPLLSCTLMLPEDGEPLTDPYEI
                     RERLEHAVDLVIDGGWCGTDPTTVIDMTDGTELVRRGFGDTAVFGL"
     gene            complement(10411..10854)
                     /locus_tag="WNA25_001590"
     CDS             complement(10411..10854)
                     /locus_tag="WNA25_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFA9824310.1"
                     /translation="MYLTQHTDYGLRVLIYTAANDDALVNIATIASTYGISKSHLMKV
                     VTALVKGGFLHSVRGKGGGLRLAAPPERINIGAVVRHLEPMQLVECMGPNNECLITPS
                     CRLTGILGGAMKSFFTYLDGFTLQDLLNKPTYDLLYESKIPIAVR"
     gene            11028..11396
                     /locus_tag="WNA25_001591"
     CDS             11028..11396
                     /locus_tag="WNA25_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HFA9824311.1"
                     /translation="MKPLKRHSALVGLSREHHHSLSLCVRMLRTPGADHRAELELHFA
                     ELETHFREEEAKFAPIWQNVAPELKTRFEGDHAKLRQMMASPECGNAAWNTAFATTLR
                     DHARFEERELFPAVEPFLPA"
     gene            11441..12595
                     /locus_tag="WNA25_001592"
     CDS             11441..12595
                     /locus_tag="WNA25_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HFA9824312.1"
                     /translation="MKFTKHPVWAMAFRPFYSLAALYGALSVLLWGFGYTGTHELSGF
                     YWHAHEMIWGYAGLVVIAFLLTAVATWTGQPPTRGGVLVGLTAFWLAARIAAFIPGWG
                     AAASGILGTLFFWYGAVCMALPVIRSQNRRNYVAVFAIFVLGGTHAAFHVQLHNGNLG
                     GLLSGLQSGLVMVSGFIGLIGMRIISFFTSKRLNVPQIPSPKWVAQASLWLPMLTAIL
                     MAHGVMPWLSAAFAFAAGVIFTVQVYRWWYKPVLKEPMLWILFAGYLFTGLGLIAVGA
                     SYFKPAFLNLGVHLIGVGGIGVLTLGMMARTALGHTGNSIYPPPKAVPVAFWLMMAAT
                     AVRMVAVFSSGTAYTHSIRTSSVLFALALLVYAWKYIPWLIRPRSDGRPG"
     gene            complement(12653..13525)
                     /locus_tag="WNA25_001593"
     CDS             complement(12653..13525)
                     /locus_tag="WNA25_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFA9824313.1"
                     /translation="MPILNHIALIGVGLIGGSFVLDLKRQGLVRTVTGIDTDRDNLER
                     ALERGVINRASVVIDADSIGGADLVLIATPVATVPAVLTALRPVLPEHTWISDVGSTK
                     SSVIEAFRRCLPDRLHRCIAAHPIAGSDRNGAQAAQFGLFRHRKLIITPHGGEDSDGI
                     ALVENLWRAVGADIFTMDAQHHDAVFAAVSHMPHLTAFAYVHQILDHPDGQEYLKFAA
                     TGFRDFTRIASGHPAVWADICLANKDSLLQLVQGLGKQLDVLADILTADDREALYRYF
                     EEAKTTRDRWLDGN"
     gene            13610..14422
                     /locus_tag="WNA25_001594"
     CDS             13610..14422
                     /locus_tag="WNA25_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HFA9824314.1"
                     /translation="MDKIRVAAVQMVSGVSPETNVAAMKRLVARAAEQGADWVLLPEY
                     WVLMGANDTGKLALAEPLGGGRFQTALSETAKECGVVLFGGTVPLQSPEAGKVMNTLL
                     VYGCDGVRTGLYHKMHLFGFSGLGERYAEADTIRAGREVPHLSAEGMPVAAGICYDVR
                     FPEFFRRQLPFDVLMLPAAFTHTTGKAHWELLLRARAVENQCYVVAAAQGGLHENGRR
                     TFGHSMIVDPWGDVLDVLPEGEGIVTADIDANRLNSVRNRLPALKHRVLDAV"
BASE COUNT         3340 a         3924 c         4054 g         3289 t
ORIGIN      
        1 cagatatatt taattttaaa taaataatat aaaaataaaa tagataataa actacatcgg
       61 attacttgaa aataattcat tgttttatat ataaaatatt ttataatttt tcaggatttt
      121 gattattgaa aatttttcta aatttcctat taattttatc ggaaacaaat aagttaaaat
      181 taatttatat taatcataat gttaaaataa aataataaaa ttatgtaatt agtctcctta
      241 aaaatgtttg acattccagt cttgtgtttt agattaccga aaaataaaac tacataatac
      301 tacaaaggaa cattactatg aaaccaattc agatgttttt cccttttctg aataatcccc
      361 ttgttttctt cttgtctgcg gttttgccgc ataattccga acggtctgct gtttttcttt
      421 gattcgtttt aaatatcaat aagataattt ttcccatata tttttaatga ttgggatgcc
      481 cgacgcgtcg gatggctgtg ttttgccgtc cgaatgtgat ggaagcctgt ccatactgaa
      541 aaaagtctat aaaggagaaa tatgatgagt caacactctg ccggagcacg tttccgccaa
      601 gctgtgaaag aatcgaatcc gcttgccgtc gtcggttgcg tcaatgctta ctttgcacga
      661 ttggccaccc aaagcggttt caaagccatc tatctgtccg gcggcggcgt ggcagcctgt
      721 tcttgcggta tccctgattt gggcattacc acaatggaag atgtgctgat cgacgcacga
      781 cgcattacgg acaacgtgga tacgcctctg atggtggaca tcgatgtggg ttggggcggt
      841 gcattcaata ttgcccgtac cattcgcaac tttgaacgcg ccggtgttgc agcggttcac
      901 atcgaagatc aggtagcgca aaaacgctgc ggccaccgtc cgaacaaagc catcgtttcc
      961 aaagatgaaa tggtcgaccg catcaaagct gccgtagatg cgcgcgttga tgagaacttc
     1021 gtgattatgg cgcgtaccga tgcgctggcg gtagaaggtt tggatgccgc tatcgaacgc
     1081 gctcaggctt gtgtcgaagc cggtgcggac atgattttcc ctgaagccat gaccgatttg
     1141 aaaatgtacc gtcaatttgc agatgcagtg aaagtaccag tattggccaa cattaccgag
     1201 tttggtgcta caccgcttta tacgcaaagc gagttggctg aaaacggcgt gtcactggtg
     1261 ctgtatccgt tgtcatcatt ccgcgcagca agcaaagccg ctttgaatgt ttacgaagcg
     1321 attatgcgcg atggcactca ggcggcggtg gtggacagta tgcaaacccg tgccgagctg
     1381 tacgagcatc tgaactatca tgccttcgat caaaaactgg ataaattgtt tcaaaaatga
     1441 tttaccgctt tcagacggcc tttcaacaaa tccgcatcga ccgtctgaaa aataaaaccc
     1501 ataaaaatac aaaggagaaa taccatgact gaaactactc aaaccccgac cttcaaacct
     1561 aaaaaatccg ttgcgctttc aggtgttgcg gccggtaata ccgctttgtg taccgttggc
     1621 cgtaccggta acgatttgag ccatcgcggt tacgacattc taaatttggc acaaaaatgc
     1681 gagtttgaag aagtcgcaca cttgctgatt cacggccatc tgcccaacaa attcgagctg
     1741 gccgcttata aaaccaagct caaatccatg cgcggcctgc ctatccgtgt gattaaagtt
     1801 ttggaaaccc tgcctgcaca tacccatccg atggacgtga tgcgtaccgg cgtatccatg
     1861 ctgggctgcg ttcatcccga acgcgaaagc catcccgaca gcgaagcgcg cgacatcgcc
     1921 gacaaactga tcgccagcct cggcagcatc ctgctctact ggtatcaata ttcgcacaac
     1981 ggcaaacgta ttgaagttga aagcgacgaa gagaccgtcg gcggccactt cctgcacctg
     2041 ttgcacggca aacgcctgac cgaatcgcag gtaaaagcca tgcacgtttc gctgattctg
     2101 tatgccgaac acgagttcaa cgcctccacc tttactgccc gcgtgattgc cggtacgggt
     2161 tcggatatgt attcctgcat caccggcgcc atcggcgcgc ttaaaggtcc gaaacacggc
     2221 ggtgcgaacg aagtcgccta cgacatccaa aaacgctacc gcaacgccga cgaagccgac
     2281 atccgcgaac gcatcggccg caaagaaatc gtgatcggtt tcggccatcc ggtttacacc
     2341 atttccgacc cgcgcaacgt cgtgattaaa gaagtggcgc gcggtttgag ccgggaagcg
     2401 ggcgacatgc gcctcttcga catcgccgaa tgcttggaga gcgtgatgtg ggaagagaaa
     2461 aaaatgttcc cgaatctgga ctggttctct gccgtttcct accaaaaatt gggcgtaccg
     2521 accgctatgt tcacaccgct gttcgtgatt tcccgtacaa ccggttggag cgcacacgtc
     2581 ctcgagcaac gcaaagacgg caaaatcatc cgtccgagcg caaactacac cggccctgaa
     2641 gatttggcgt ttgtggagat tgaagaacga taattgaaga atgcaatagc agtttgttct
     2701 ttatttcggt atgcaaagct gaggatttca gacgaccttg ccttattgga aaggttgtct
     2761 gaaataagtt taatctaata ggagaagata atcctgtatt ggcgcaagta acaggataag
     2821 aaacatggaa gatttataca taatactcgc tttgggtttg gttgcgatga tcgccggatt
     2881 tatcgatgcg attgcgggcg ggggtggttt gattacgctg cctgcactct tgttggcagg
     2941 tattcctccc gtgtcggcaa ttgccaccaa caagctgcaa gcagccgctg ctacgttttc
     3001 ggctacggtt tcttttgcac gcaaaggttt gattgattgg aagaaaagtc tcccgattgc
     3061 cgcagcatcg tttgcaggcg gcgtggtcgg tgcattatcg gtcagcttgg tttccaaaga
     3121 tattttgctg gcggtcgtgc cggttttgtt gatatttgtc gcgctgtatt ttgtgttttc
     3181 gcccaagctc gacggcagta aggaaggcaa agccagaatg tctttttttc tattcgggct
     3241 gacggttgca ccgcttttgg gtttttacga cggtgtgttc ggaccgggtg tcggctcgtt
     3301 ttttctgatt gcctttattg ttttgctcgg ctgcaagctg ttgaacgcga tgtcttacac
     3361 caaattggcg aacgttgctt gcaatcttgg ttcgctatcg gtattcctgc tgcacggttc
     3421 gattattttc ccgattgtgg caacgatggc ggtcggtgcg tttgtcggtg cgaatttagg
     3481 tgcgagattt gccgtccgct tcggttcgaa gctgattaag ccgctgctga ttgtcatcag
     3541 catttcgatg gctgtgaaat tgttgataga cgagagaaat ccgctgtatc agatgattgt
     3601 ttcgatgttt taaacccttt cagacgactc cttcaaaaca tcggctgaaa cccaaaccac
     3661 aagaaaaata gatccacagg agaaccgaca tgactgccaa ccaaagttac cgcaaaccgc
     3721 tgcccggtac ggatttggaa tactacgacg cgcgtgcggc gtgtgaggac atcaaacccg
     3781 gctcttacga caagctgccg tacacgagcc gcattttgtc tgagaacttg gtcaaccgcg
     3841 cggacaaagt cgatttgccg atgctgcaaa gctggctggg ccagttgata gaagggaagc
     3901 aggaaatcga ttttccgtgg tatcccgcgc gggtggtgtg ccacgatatt ctggggcaga
     3961 ccgcgctggt ggatttggca ggcttgcgcg atgcgattgc cgaaaaaggc ggcgatcctg
     4021 ccaaagtgaa tccggtggtg cagacgcagc ttatcgtcga ccactctttg gcggtggagt
     4081 gcggcggtta cgatcctgat gccttccgca aaaaccgcga aatcgaagac agacgtaacg
     4141 aagaccgttt ccacttcatc aactggacaa aaaccgcatt tgaaaatgtg gacgtgattc
     4201 cggcgggcaa cggcatcatg caccaaatca atctggaaaa aatgtcgccc gtcgtccaag
     4261 tcaaaaacgg cgtggctttc cccgatacct gcgtcggcac ggactcgcat acgccgcacg
     4321 ttgatgcgct gggcgtgatt tccgtggcgt gggcggtttg gaagcggaaa ccgtgatgct
     4381 gggacgcgcg tccatgatgc gcctgcccga tattgtcggc gtggagctga cgggcaaacg
     4441 gcaggcgggc attactgcca cagacatcgt gttggcactc accgaattct tgcgtaaaga
     4501 gcgcgtggtc ggggcgtttg tcgaattttt cggcgagggc gcgagaagcc tgtctatcgg
     4561 cgaccgcgcg accatttcca acatgacgcc ggagttcggc gcgactgccg ccatgttcgc
     4621 catcgacgcg caaactattg attatttgaa actgaccgga cgtgacgacg cgcaggtgaa
     4681 attggtggaa acctacgcca aaaccgcagg cttatgggca ggtggcttga aaaccgccgt
     4741 ttatccgcgc gttttgaaat ttgatttgag cagcgtaacg cgcaatatgg caggcccgag
     4801 caacccgcac gcgcgttttg ccaccgccga tttggcggcg aaagggctgg cgaagcctta
     4861 cgaagagcct tcagacggcc aaatgcctga cggtgcagtg attattgccg cgattacttc
     4921 gtgtaccaat acttccaacc cgcgcaacgt tgtcgccgcc gcactgttgg cacgcaatgc
     4981 caaccgcctc ggcttgaaac gcaaaccttg ggtgaaatct tcgtttgccc cgggttcaaa
     5041 agtagccgga atctatttga aagaagcagg cttgttgccc gaaatggaaa aactcggctt
     5101 cggtatcgtc gccttcgcat gtaccacctg taacggcatg agcggcgcgc tcgacccgaa
     5161 aatccaacaa gaaatcatcg accgcgattt gtacgccacc gccgtattgt caggcaaccg
     5221 caacttcgac ggccgtatcc atccgtatgc gaaacaggct ttcctcgctt cgcctccttt
     5281 ggtcgttgcc tacgcattgg caggtagcat ccgtttcgat attgaaaacg acgtactcgg
     5341 cgttgcagac ggccgcgaaa tccgcctgaa agatatctgg ccgacagacg aagaaatcga
     5401 tgccatggtt gccgaatatg tgaaaccgca acaattccgc gacatttata tcccgatgtc
     5461 cgacaccggc acagcgcaaa aagcaccaag cccgctgtac gactggcgac cgatgtccac
     5521 ctacatccgc cgtccgccct attgggaagg cgcactggca ggggaacgta cattaagagg
     5581 tatgcgtccg ccggcgattt tgcccgacaa catcaccacc gaccacatct cgccatccaa
     5641 tgcgattttg gccggcagtg ccgcaggcga atatttggcg aaaatgggtt tgcctgaaga
     5701 agacttcaac tcttacgcaa cccaccgcgg cgaccacttg accgcccaac gcgcaacctt
     5761 cgccaatccg aaactgttta acgaaatggt gagaaacgaa gacggcagcg tacgccaagg
     5821 ttcgttggca cgcgttgaac cagaaggcca aaccatgcgc atgtgggaag ccatcgaaac
     5881 ctatatgaac cgcaaacagc cgcttatcat cattgccggt gcggactatg gtcaaggctc
     5941 aagccgcgac cgggcggcga agggcgtgcg gctggcgggt gtggaagcca tcgccgccga
     6001 aggtttcgag cgcatccacc gcaccaacct catcggcatg ggcgtcttgc cgctgcaatt
     6061 caaacccggc accaaccgcc ataccctgca actggacggt acggaaacct acgacgttgt
     6121 cggcgaacgc acaccgcgct gcggcctgac cctcgtgatt caccgtaaaa acggagaaac
     6181 cgtcgaagtt ccggttacct gccgccccga tactgcagaa gaagtattgg tatatgaagc
     6241 cggcggcgtg ttgcaacggt ttgcacagga ctttttggaa gggaacgcgg cttagagttc
     6301 gtctgaaaag caagacgtag cgtgggtcgg gttctcattc acgtaattcc cgatatggca
     6361 ggcatttact gtaaatcgtc attcccgcgc aggcgggaat ccagaaagtg gaattgagga
     6421 aaccttttta tccgatgagt ttctgtgcgg ataaatctgg atttccgcct acgcgggaat
     6481 gacggggttt aataatctac cgtatcacaa cacagcagcc gtagattggg gcgaaccccg
     6541 acagtttgcg gaatcaaacg gctttggtcg gagtggtagc ctaatgtact tctggaaagt
     6601 gggtgtagcg tgggctttgc ccacgaaata aaggctgaat tgacatggta tagcggatta
     6661 acaaagatca ggacaaggcg gcgggccgca ggcagtacaa atggtacgga accggttcgc
     6721 ccggtgcttg ggcgccttag ggaatcgttc cctttgagcc ggggcggggc aacccgtacc
     6781 ggtttaaatt aatccactat aaaaatcgtg ggcagagccc acgctacata aggagaaccc
     6841 taaaatgccg caaatcaaaa tccccgccgt ttactaccgc ggcggtacat ctaaaggcat
     6901 tttcttcaaa cgcaccgatc tgccggcagc tgcgcaggaa gcaggcgggg cacgcgacaa
     6961 aatcctcttg cgcgtactcg gcagccccga cccttacggc aaacagattg acggtttggg
     7021 caacgccagt tcgtccacca gcaaagctgt gatttcggac aaatccgaac gcgccgatca
     7081 cgatgtcgat tacttgttcg gacaagtttc catcgacaaa ccatttgtcg attggagcgg
     7141 caactgcggc aaccttaccg ccgccgtggg cacattcgcc atcgaacagg gcttggtcga
     7201 taaatccaaa atcccttcag acggcatctg cacggtcaaa atctggcaga aaaacatcgg
     7261 caaaaccatt attgcccatg tgccgatgca aaacggagca gtgttggaaa caggcgattt
     7321 cgagcttgac ggcgtaattt tccggcagcc gaagtacaaa tcgaattttt agatccagcc
     7381 gacggcgaag gcagtatgtt cccaaccggc aatttggtcg acgaaattga tgtgccgaat
     7441 ataggtcgtc tgaaagccac gctcatcaac gcgggcattc cgaccgtttt cctgaacgcc
     7501 gccgacttgg gctacacggg caaagagttg caggacgaca tcaacaacga tgccgccgcg
     7561 ctggaaaaat ttgaaaccat ccgcgcatat ggcgcgctga aaatgggttt gatcagcgac
     7621 gtatccgaag ccgccgcccg cgcgcacacg ccgaaacccg ccttcgtcgc gcccgccgcc
     7681 gattacaccg cctccagcgg caaaaccgta aacgccgccg acatcgattt gccggtacgc
     7741 gccctgagca tgggcaaact gcaccacgct atgatgggca tcgcctcggt cgccatcgcc
     7801 gccgccgtgc tcggtacgct ggtcaacctt gccgcaggcg gcggaacgcg taaagaagtg
     7861 cgcttcgggc atccgtcagg tacgctgcgt gtcggtgctg ccgccgaatg tcaggacgga
     7921 caatggacgg ccgccaaagc ggtcatgagc cgcagcgcac gcgtgattat ggaaggttgg
     7981 gtgcgcgttc ccgatgattg tttttgaaat catgtaggac gcacattgct gtccgctgtt
     8041 tcagacggca tttgattaag gattaccata aagccgaata accttaccgc cgtcattccc
     8101 acgcaggcgg gaatccgcag ccgatattct gtttcctgat ttttatcaga gcctgcgtgt
     8161 gagcaaatat ggattcccgc ctgcgtggga atgacgctag gaaatacggc atacgctttg
     8221 cccaaagagg ccgtctgaaa cacactccgc ccaacgccca tcctttttca gacggccccg
     8281 caccaaaaaa acaaccacaa actacaagga gaaacatcat gtccgaccaa ctcattcttg
     8341 tcctgaactg cgtcagttca tcgctcaaag gcgccgttat cgaccgcaaa agcggcagcg
     8401 tcgtcctaag ctgcctcggg gaacgcctga ctacgcccga agccgtcatt accttcaaca
     8461 aagacggcaa caaacgccaa gttcccctga gcggccgcaa ctgccacgcc ggcgcggtgg
     8521 gtatgctgtt gaacgaactg gaaaaacacg gactgcacga ccgcatcaaa gccatcggcc
     8581 gccgcatcgc ccacggcggc gaaaaatatc acgagtccgt cctcatcgac caagacgtcc
     8641 ttgacgaact gaaagcctgc atcccgttcg ccccgctgca caaccccgcc aacatcagcg
     8701 gcatcctcgc cgcgcaggaa cactttcccg gcctgcccaa cgtcggcgtg atggacacct
     8761 cgttccacca aaccatgccg gagcgggcct acacttatgc cgtgccgcgc gaattgcgca
     8821 aaaaatacgc cttccgccgc tacggtttcc acggtaccgg tatgcgttac gtcgcccctg
     8881 aagccgcacg catcttgggc aaacctctgg aagacatccg catgattatt gcccacttag
     8941 gcaacggtgc atctattacc gccgtcaaaa acggcaaatc cgtcgatacc ggtatgggtt
     9001 tcacgccgat cgaaggtttg gtaatgggta cacgttgcgg cgacaccgat ccgggcgtat
     9061 acagctatcc gactttccac gcagggatgg atgttgccca agttgatgaa atgctgaacg
     9121 aaaaatcagg tttccccggt atttccgaac ttcccaacga ctgccgcacc ctcgaaatcg
     9181 ccgccgacga aggccgcgaa ggcgcgcgcc tcgccctcga agtcatgacc tgccgcctcg
     9241 ccaaatacat cgcttcgatg gctgtggcct gcggcagtgt tgacgcactc gtgttcaccg
     9301 gcggtatcgg cgaaaactcg cgtaatatcc gtgccaaaac cgtttcctat cttgatttct
     9361 tgggtctgca catcgacacc aaagccaata tggaaaaacg ctacggcaat tcgggcatta
     9421 tcagcccgac cgattcttct ccggctgttt tggtcgtccc gaccaatgaa gaactgatga
     9481 ttgcctgcga cactgccgaa cttgccggca tcttgtagcc aaaaaagggg gcgagtccgc
     9541 aaaaatgccg tctgaaaggt tttcagacgg cattttgttt ggcgtttggg cgtacaatac
     9601 ggaaaacatg acgataagga aacaaactat ggcacaattt ttcgccgttc atcccgacaa
     9661 tccccaagaa cgcctcatca agcaggcagt cgaaatcatc aatcaaggcg gcgtggtggt
     9721 ttatccgacc gattcctgtt acgccttggg ttgcaaactc ggcgataagg cggcgatgga
     9781 acgcatactc tccatccgaa aaatcgattt gaaacaccac ctgaccctga tgtgcgccga
     9841 tttgagcgag ttgggcacat acgccaaaat cgacaacgta cagtttcgtc agcttaaagc
     9901 cgccacgccc gggccttata cctttatctt gcaggcgacg aaggatgcgc cggcgcgcgc
     9961 cctgcacccg aaacgcaaaa ccatcgggct gcgtattccc gataatgccg ttgcacaagc
    10021 cctgctggag gagttgggcg ggccgctttt aagctgcacc ctgatgctgc ccgaagacgg
    10081 cgaaccattg accgaccctt atgaaatccg cgagcgtttg gaacacgccg tcgatttggt
    10141 aattgacggc ggctggtgcg gaaccgatcc gactaccgtc atcgacatga ccgacggcac
    10201 ggaactggtg cgccggggtt tcggcgatac ggcggtgttc ggtttgtagg gaaaccgatg
    10261 ccgtctgaag catcagccgt tcagacggca tcgcgcgcct tgccggcggc agcccgaaat
    10321 gccggcgcgt atcgcgcccg gtcggaatat ccgtttgaaa cggcaaacaa aatgccgtcc
    10381 gaacgcgggt ttgggcggca ttttgatgcg ttatcgcacc gcaatcggga ttttcgattc
    10441 ataaagcagg tcgtaggtcg gcttgttgag caggtcttgc agcgtgaaac cgtccagata
    10501 cgtgaaaaac gacttcattg caccgccgag tatgcccgtc agccggcagg acggcgtaat
    10561 caggcattcg ttgttcgggc ccatacactc gaccagctgc atcggttcga ggtggcggac
    10621 gaccgcgccg atattgatgc gttcgggcgg cgcggccagc ctcagcccgc cgcctttccc
    10681 gcgtacgctg tgcaagaacc cgcctttgac cagcgcggta accactttca tcaaatggct
    10741 tttggaaatg ccgtaggtcg aggcgatggt ggcgatattg accagcgcgt cgtcgttggc
    10801 ggcggtgtag atgaggacgc gcagcccgta gtcggtatgt tgggtcagat acatgatttt
    10861 ctcggtatgg atcgttattc ttatcggtac ggtttaagtt tcacggaaaa taccttaatg
    10921 gttgaaaccc tgtccgtcgg ggcggtagaa tgtagcctgt ctgcggcggt atgccgtctg
    10981 aaacatccgc gctaccgttt gataatttgt tattgtaacc taaatttatg aaaccgttga
    11041 aacgacattc cgcccttgtc gggctttcgc gcgaacacca ccactcgctt tccctgtgcg
    11101 tgcgtatgtt gcggacgccc ggggcagacc atcgtgccga actcgagctg cattttgccg
    11161 aactggaaac ccattttcgc gaagaagaag ccaagtttgc cccaatttgg cagaatgtcg
    11221 cgcctgagtt gaaaacccgt tttgaaggcg atcacgccaa actgcggcag atgatggcaa
    11281 gccccgaatg cggtaacgcg gcgtggaata ccgcttttgc cacaaccctg cgcgaccacg
    11341 cgcgctttga agaacgcgag ctgtttcccg ccgtcgaacc gtttttgccg gcatgattcc
    11401 gttttgcggc aaatatatta atgataaaca aggaacacac atgaaattta ccaaacatcc
    11461 cgtctgggca atggcgttcc gcccgtttta ttcactggcg gcactgtacg gcgcattgtc
    11521 cgtattgctg tggggtttcg gctacacggg aacgcacgag ctgtccggtt tctattggca
    11581 cgcgcatgag atgatttggg gttatgccgg tctcgtcgtc atcgccttcc tgctgaccgc
    11641 cgtcgccact tggacgggac agccgcccac gaggggcggc gttctggtcg gcttgaccgc
    11701 cttttggctg gctgcgcgga ttgccgcctt tatcccgggt tggggtgcgg cggcaagcgg
    11761 catactcggt acgctgtttt tctggtacgg cgcggtgtgc atggctttgc ccgttatccg
    11821 ttcgcaaaac cggcgcaact atgtcgccgt attcgcaata tttgtgctgg gcggtacgca
    11881 tgcggcgttc cacgtccagc tgcacaacgg caacctaggc ggactcttga gcggattgca
    11941 gtcgggcctg gttatggtgt cgggctttat cggcctgatt gggatgagga ttatttcgtt
    12001 ttttacgtcc aaacggttga acgtgccgca gattcccagt ccgaaatggg tggcgcaggc
    12061 ttcgctgtgg ctacccatgc tgaccgccat actgatggcg cacggcgtga tgccttggct
    12121 gtcggcggct ttcgcgtttg cggcgggcgt gatttttacc gtacaggtgt accgctggtg
    12181 gtataaaccc gtattgaaag aaccgatgct gtggattctg tttgccggct atctgtttac
    12241 cggattgggg ctgattgcgg tcggcgcgtc ttatttcaaa cctgccttcc tcaatctggg
    12301 cgtacatctg atcggggtcg gcggtatcgg cgtgctgact ttgggcatga tggcgcgtac
    12361 cgcgctcggt catacgggca attcgattta tccgccgccc aaagccgttc ccgttgcgtt
    12421 ttggctgatg atggcggcaa ccgccgtccg tatggttgcc gtattttctt ccggcactgc
    12481 ctacacgcac agcatccgca cgtcttcggt tttgtttgca ctcgcgctgc tggtgtatgc
    12541 gtggaaatac attccgtggc tgatccgtcc gcgttcggac ggcaggcccg gttgagtaaa
    12601 accgccgcag atttcgggtc tgcggcggtt tgcttttcag acggcatggc ggtcagttgc
    12661 cgtccagcca gcggtcgcgc gtggttttgg cttcttcaaa atagcggtac agggcttcgc
    12721 ggtcgtcggc ggtcaggatg tctgccaaaa cgtccaactg tttgcccaag ccttgaacca
    12781 gttgcagcag gctgtctttg ttggcaaggc agatgtccgc ccacacggcg ggatggccgg
    12841 aggcgatgcg ggtgaagtcc cgaaagcccg tggcggcgaa tttcagatat tcctgtccgt
    12901 cggggtggtc gagaatctgg tggacatagg cgaaggcggt caggtggggc atatgggaga
    12961 cggcggcgaa aaccgcgtcg tggtgttgcg cgtccatcgt gaaaatgtcc gcaccgaccg
    13021 cgcgccacag gttttctacc aatgcgatgc cgtctgaatc ttcgccgccg tggggcgtga
    13081 tgatgagttt tctgtggcgg aacagcccga actgcgcggc ttgcgcaccg tttctgtccg
    13141 aaccggcaat cgggtgtgcg gcgatgcagc ggtgcaggcg gtcgggcagg cagcggcgga
    13201 aggcttcgat gaccgaagat ttggtgctgc cgacatcgga aatccaagtg tgttccggca
    13261 aaacggggcg cagcgcggtt aaaacggcgg gaacggtggc gacgggcgtg gcaatcagta
    13321 ccaagtccgc accgccgatg ctgtccgcgt cgatgacgac ggaagcccgg ttaatcacgc
    13381 cgcgttccaa tgcacgttcg aggttgtcgc ggtcggtgtc gataccggta acggtgcgga
    13441 cgagtccctg ccttttgagg tcgagaacga acgaaccgcc gatcagccct acaccgatga
    13501 gggcaatatg gttcaaaatg ggcatttgtg taaacggttt tcgcaaagtg ccgccatggt
    13561 agcctatcgg cggaatatgc cgcaaggtcg gcaggaaaaa ggagaagaaa tggacaaaat
    13621 cagagttgcc gccgtgcaga tggtgtcggg cgtgtcgccg gaaaccaacg tcgccgccat
    13681 gaaacgcctg gtcgcacggg cggcggagca gggtgcggat tgggtgctgc tgcccgaata
    13741 ttgggtgctg atgggcgcaa acgataccgg caaactcgca cttgccgagc ctttgggcgg
    13801 cggacgcttt cagacggcat tgagcgaaac ggcgaaagaa tgcggcgtgg tgctgttcgg
    13861 cggaaccgtg ccgctgcaaa gccccgaagc gggcaaggtc atgaatactt tattggtgta
    13921 cgggtgcgac ggcgtaagga cggggctgta ccacaaaatg cacctcttcg gtttttccgg
    13981 tttgggcgaa cgctatgccg aagccgatac catccgcgca gggcgggaag tgccgcactt
    14041 gtcggcagaa ggcatgccgg tggcggcagg tatttgttac gatgtccgct ttcccgaatt
    14101 tttccgccgc caactgccgt ttgacgtatt gatgctgccg gcggcattta cccatacgac
    14161 gggcaaggcg cactgggagc tgctgctgcg cgcgcgcgcc gtcgaaaacc aatgttacgt
    14221 cgtggcggcg gcacagggcg gtttgcacga aaacggacgg cgcacgttcg gacacagtat
    14281 gattgtcgat ccgtggggcg acgtgttgga cgtattaccc gaaggcgaag gcatcgttac
    14341 ggcagacatc gatgccaacc gcctgaacag cgtccgcaac cgcctgcccg ccttgaaaca
    14401 ccgggttttg gatgccgtct gaaggttcag acggcatcgg tgtcggggaa tcagcgggcc
    14461 gtagcaaccg aaccgtcatt cccacggaag tgggaatcta ggacgcgggg tttcagttat
    14521 ttccgataga ttcccgccgc gtcgggggtc cggatttccg cctgcgcggg aatgacgggt
    14581 ttcgagattg cggtgttgtc ggacggg
//
LOCUS       DAWTQS010000054        14270 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.054, whole genome shotgun sequence.
ACCESSION   DAWTQS010000054 DAWTQS010000000
VERSION     DAWTQS010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14270
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.054"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            870..1979
                     /locus_tag="WNA25_001595"
     CDS             870..1979
                     /locus_tag="WNA25_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFA9824315.1"
                     /translation="MSNWKPDIPYNDLPPLPPKQDIESKTILKRCIAARASLARLKQA
                     AELIPNQAMLINTLPVMEARASSEIENIVTTTDKLFQSLQMDTERQDPATKEALQYRT
                     ALFAGYESLASRPLCTQTAIMVCNAIKHPYETAIRKTGGTALKGGNSGNVVYTPPEGE
                     ETIRGKLANWERFIHESGDLDPLVIMAAAHYQFEAIHPFTDGNGRTGRILNSLLLIEK
                     GLLDLPILYLSRYIIENRADYYRLLLGVTERQDWESWIIYILDGVADTADWTVSKIDA
                     IRCLFEQTRQHIRTHAQGIYTHELVNLLFEQPYTRIANLEAAGIAKRQTASKYLKELS
                     GIGVLQEIAIGRDKLFIHPRLMELLRGEGNSFTSF"
     gene            complement(2708..3520)
                     /locus_tag="WNA25_001596"
     CDS             complement(2708..3520)
                     /locus_tag="WNA25_001596"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFA9824316.1"
                     /translation="MENIGRQRPIGVFDSGIGGLTNVRALMERLPMENIIYFGDTARV
                     PYGTKSKATIENFSMQIVDFLLGHDVKAMVIACNTIAAVAGRKIRQKTGNMPVLDVIS
                     AGAKAALATTRNNKIGIIATNTTVNSNAYARAIHRDNPDTLVRTQAAPLLVPLVEEGW
                     LEHEVTRLTVCEYLKPLLADGIDTLVLGCTHFPLLKPLIGREAHNVALVDSAITTAEE
                     TARVLAQEGLLDTGNNNPDYRFYVSDIPLKFRTIGERFLGRTMEQIEMVSLG"
     gene            3708..4169
                     /gene="tsaE"
                     /locus_tag="WNA25_001597"
     CDS             3708..4169
                     /gene="tsaE"
                     /locus_tag="WNA25_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFA9824317.1"
                     /translation="MSDFSPVSRFLADEAATLDLGAAWSSRLNAPLVIYLEGDLGAGK
                     TTLTRGILRGLGHQGAVKSPTYAIVESYPLERFALHHFDLYRFSFPEEWEDAGLDELF
                     AANSVCLIEWPQQGGEFTPPADITATLTHGGGGRKCLLTAHTERGRESLPL"
     gene            4166..5416
                     /locus_tag="WNA25_001598"
     CDS             4166..5416
                     /locus_tag="WNA25_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFA9824318.1"
                     /translation="MTKLTRRQIIRRTAGTLFALSPIASAVAKTVRAPQFTAARIWPS
                     HTYTRLTLESTAALKYQHFALDNPGRLVVDIQNANINTVLHGLSQKVMADDPFIRSIR
                     AGQNTPTTVRLVIDLKQPTHAQVFALPPVGGFKDRLVVDLYPHGMDADDPMMALLNGS
                     LNKTLRGSPEADPAQNTTPRPGRGKNGRRPVIMLDPGHGGEDPGAVSPGGLQEKHVVL
                     SIARETKKQLEALGYNVFMTRNEDVFIPLGVRVAKGRARRADVFVSIHADAFTSPSAR
                     GTGVYMLNTKGATSSAAKFLEQTQNNADAVGGVPTSGNRNVDTALLDMTQTATLRDSR
                     KLGKLVLEELGRLNHLHKGRVDEANFAVLRAPDMPSILVETAFLSNPAEEKLLGSESF
                     RRQCAQSIASGVQRYINTSVLKRG"
     gene            5498..6316
                     /locus_tag="WNA25_001599"
     CDS             5498..6316
                     /locus_tag="WNA25_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFA9824319.1"
                     /translation="MKGMDKLRYQRDFLNIRPIFTAGEQEYLTELSDRLPLSVLTDSV
                     RNIEEIGIDFVYSPAKLEGNTYNQYDTQALLKLGQTAGGKLYSDAVMLINLRESYRHL
                     LSGLDSPKPFDWLDFLKTTHSLISENLLEKGSGGVVRRDSVTISGTDYTPLSNPQSLD
                     TELKWLLQEAPKIENPFDRAVYLHNNLAYLRYFKDCNKRTARNCMTLSLMRSGFFPCV
                     FSPDSYPAYAEAVVAYYETGDYGLFKKYFISAYENTVNKYGPQPDVDIFRNFSI"
     gene            complement(6362..7504)
                     /locus_tag="WNA25_001600"
     CDS             complement(6362..7504)
                     /locus_tag="WNA25_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFA9824320.1"
                     /translation="MNTLYTLFATCPRGLETVLSQELESLGCTEVRAFDGGVSCRGGL
                     EQAYAANLHSRTAGRILLRLTKGTYRTERDIYKLAKNINWFNWFTLQQTFKVKIEAKR
                     ANVKSLQFAGLTVKDAVCDAFRDIYDARPSVDKAAPDVRIHAFLDERNVEIFIDTSGE
                     ALFKRGYRLDTGEAPLRENLAAGLLLSAGYDGTQPFQDPFCGSGTIAIEAAWIAAGRA
                     PGMMRRFGFEKLQNFDKTLWADLRRRAEAQTRPVRAPIAGSDNGRRIVQTALDNAHNA
                     GADDIVSFSVADAQSVRPNGENGIMVSNPPYGVRLEEVRALQALYPQLGTWLKKHYAG
                     WRVGMFTGDREMPKFMRLLPKRKIPLYNGNIDCRLFLIDMVQGSNR"
     gene            complement(7553..7975)
                     /locus_tag="WNA25_001601"
     CDS             complement(7553..7975)
                     /locus_tag="WNA25_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824321.1"
                     /translation="MNKNRKLLLAVLLLTAFAAFKLVLLQWWQAQQPQAVAAQCDLTE
                     GCTLPDGSRVRAAAVSTKKPFDIYIEHAPAGTEQVSISFSMKNMDMGFNRYMFERQPS
                     GNWQAARIRLPVCVEGRRDFTADITIGSRTFQTAFTAE"
     gene            complement(7968..8777)
                     /locus_tag="WNA25_001602"
     CDS             complement(7968..8777)
                     /locus_tag="WNA25_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFA9824322.1"
                     /translation="MIQDTRPLIRVVAGILLDSDGNYLLGSRPEGKPYAGYWEFAGGK
                     VEAGETDFQALQREFGEELGIRILAATPWLTKIHSYEHARVCLKFLWVNPDQWEGGPQ
                     SREGQEWSWQKAGDFTVAPMLPANGALLRSLSVPRRLYGSLKTGLYGENSMGAYRVLP
                     LGSAGGSGANVLMEAAQWQDRSEHAGSVWMMVQTREQWRQAQEKGADVVVWRVCDDVQ
                     AQEAAEALRQGVSVPLVLAANGQTVARYGKLWLGLGAHAVVRDETIGKNHE"
     gene            complement(8859..9236)
                     /locus_tag="WNA25_001603"
     CDS             complement(8859..9236)
                     /locus_tag="WNA25_001603"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFA9824323.1"
                     /translation="MIYGIGTDIVSLKRIIRLNKKFGQAFAGRILTPEELLEFPQAGK
                     PVNYLAKRFAAKEAFAKAVGTGIRGAVSFCNIGIGHDALGKPEFFYGPALSEWLEEQG
                     ISRVSLSMADEGDTVLAFAVAEK"
     gene            complement(9286..10014)
                     /gene="pdxJ"
                     /locus_tag="WNA25_001604"
     CDS             complement(9286..10014)
                     /gene="pdxJ"
                     /locus_tag="WNA25_001604"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFA9824324.1"
                     /translation="MLLGVNIDHIATVRNARGTTYPSPVEAALVAETHGADLITMHLR
                     EDRRHIKDADVFAVKNAIRTRLNLEMALTEEMLENALKVMPEDVCIVPEKRQEITTEG
                     GLDVLAQQEKIAEFAKILTDAGIRVSLFIDADDRQIQAAYDVGAPVVELHTGAYADAQ
                     SHAEQIRQFERIQNGAHFAGDLGLVVNAGHGLTIHNVTPVAQILAIRELNIGHSLIAQ
                     ALFLGLPEAVRQMKEAMFRARLLP"
     gene            complement(10067..10810)
                     /gene="recO"
                     /locus_tag="WNA25_001605"
     CDS             complement(10067..10810)
                     /gene="recO"
                     /locus_tag="WNA25_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFA9824325.1"
                     /translation="MSEYRVNHEPVFMLASSPWRESSLRVEAFSRRYGRVALLARSAR
                     KRQSELRGVLVPFVPASVSWYGSQELKTLHRAEWMGGWRQPQGRALFSGLYVNELVLK
                     LTAREDPMSELYDALAKVMEAVCREANHIADLRRFEWKLLNALGVAPDLHADGTGGDI
                     LADKTYRLMPEEAVMPVCRDTGALSHEAGAIVEGQSLIDLREGSFRTAESLQQALKIT
                     RLLIGTLLPEGLKSRQVLEQIRQFDRNTA"
     gene            complement(10847..11974)
                     /gene="pheA"
                     /locus_tag="WNA25_001606"
     CDS             complement(10847..11974)
                     /gene="pheA"
                     /locus_tag="WNA25_001606"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFA9824326.1"
                     /translation="MLECTANYRSGEIMSQTIDELLLPHRNAIDTIDAEILRLLNERA
                     QHAHAIGELKGTGAVYRPEREVAVLRRIQDLNKGPLPDESVARLFREVMSECLAVERP
                     LTIAYLGPQGTFTQQAAIKHFGHAAHTMACPTIDDCFKQVETRQADYLVAPVENSTEG
                     SVGRTLDLLAVTALQACGEVVLRIHHNLLRKNNGSTEGIAKVFSHAQALAQCNDWLGR
                     RLPNAERIAVSSNAEAARLVAESDDGTVAAIAGRTAAEIYGLDMVAECIEDEPNNTTR
                     FLVMGHHETGASGSDKTSLAVSAPNRAGAVASLLQPLTESGISMTKFESRPSKSVLWE
                     YLFFIDIEGHRRDAQIQTALERLGERASFVKAIGSYPTAVL"
     gene            complement(12013..13050)
                     /locus_tag="WNA25_001607"
     CDS             complement(12013..13050)
                     /locus_tag="WNA25_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HFA9824327.1"
                     /translation="MFGTAFGQVAGGAVSDIKGRKPVALTGLIVYCLAVAAIVFASST
                     EQLLNLRAVQAFGAGMAVVIVGAMVRDYYSGRKAAQMFALIGIILMVVPLAAPMVGAL
                     LQGLGGWRAIFVFLAAYSPVLPGLVQYFLPNPAVGGKIGRDVFGLVAGRFKRVLKTRA
                     AMGYLFFQAFSFGSMFAFLTESSFVYRQLYHVTPHRYAWVFALNIITMMFFSRVTAWR
                     LKTGAHPQSILLRGIVVQFAANPSQLAAVLFFGLPPFWLPVACVMFSVGTQGLVGADT
                     QACFMSYFKEEGGSANAVSGVFRSLIGAGVVMAATVMAATMTASASCGIALLWLCSHR
                     AWKENGQSEYL"
     gene            complement(13066..13344)
                     /locus_tag="WNA25_001608"
     CDS             complement(13066..13344)
                     /locus_tag="WNA25_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824328.1"
                     /translation="MPSEAVSGGISFGFAPPSDMLNLRPCRPSFATVSLFDLNGKTMS
                     PTLPPMSGKLMAVLMAVLVALMPFSTDAYLPAIPEMAQPLNADIHRIE"
     gene            complement(13334..14209)
                     /locus_tag="WNA25_001609"
     CDS             complement(13334..14209)
                     /locus_tag="WNA25_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFA9824329.1"
                     /translation="MFYQILALIIWSSSFIAAKYVYGGIDPALMVGVRLLIAALPALP
                     ACRRHVGKIPREEWKPLLIVSFVNYVLTLLLQFVGLKYTSAASASVIVGLEPLLMVFV
                     GHFFFNDKARAYHWICGAAAFAGVALLMAGGAEEGGEVGWFGCLLVLLAGAGFCAAMR
                     PTQRLIARIGAPAFTSVSIAAASLMCLPFSLALAQSYTVDWSVGMVLSLLYLGLGCGW
                     YAYWLWNKGMSRVPANASGLLISLEPVVGVLLAVLILGEHLSPVSALGVFVVIAATFA
                     AGRLSRRDAQNGNAV"
BASE COUNT         3295 a         4086 c         3835 g         3054 t
ORIGIN      
        1 ctgccctctc tctaactctc tcccgcaggg agagaggacg gtgcggctgt tggggttaag
       61 gtttatgcaa actaatacgg cttgctgccc tctctctaac tctctcccac ggggagagag
      121 gactatgatg cccaccggcg gcttgctatt aggaatatct gaatcgtcat tcccacgaaa
      181 gtgggaatct agaacgtcaa atctcaagaa accgttttat ccgataagtt tctgttccga
      241 caggtccgga ttcccgcctg cgcgggaatg acgaatccat ccatacggaa acctgcatcc
      301 cgtcattccc acgaacctac attccgtcat tcccacgaaa gtgggaatct agttttttga
      361 gtttcagtca tttccgataa attgccttag cattgaaggt ccggattccc gcctgcgcgg
      421 gaatgacggg atttgaggtt tctgttttta attttctgtt ttcacgggaa tgacggttta
      481 aaagttaccc gaaacctcaa aaaaaaaaag ctgtgtttta atatagtgtt gatagacgta
      541 cttggcctcc atgtattcaa tcgtggaaat ctatatcttc gtcctcgccg aaatagtcta
      601 tgcccgatat acaattttga tacacaaact tggaaatatg ggtatcgtcg ccggagcgat
      661 agaatgcgga cagttatata tatacggttt ttttagggga gcgggcagat gaagggcgga
      721 ttttgaatta accccatgca aattgacttt tgcgggcggt tttaccgccc atatatttac
      781 aaaagccaaa tttctaaaca tatatccttg atatatacac ggcgtaaaca tatactggaa
      841 acatctttaa attttccgaa attttaaaca tgagcaactg gaaacccgat attccctata
      901 acgatttacc acccctgccg ccaaaacagg atattgaaag caaaaccatc ctgaaacgtt
      961 gtatagccgc ccgtgcatcc cttgcccgtt taaagcaggc ggcagaattg ataccgaatc
     1021 aagccatgct gattaacacc cttcctgtta tggaagcccg tgcaagttcg gaaattgaaa
     1081 acatcgtaac caccacggac aagctgtttc aatccctgca aatggatacg gaacggcaag
     1141 accctgccac gaaagaagcc ctgcaatacc gcaccgccct gtttgcaggc tatgaatcac
     1201 tggcgagccg ccctttatgc acacaaaccg ccatcatggt ctgcaacgcc atcaagcacc
     1261 cctacgaaac ggccatccgc aaaacaggcg gcacagccct aaaaggaggt aacagcggaa
     1321 atgttgtcta taccccgccc gaaggagaag aaaccatacg cggcaagctg gcaaattggg
     1381 agcggtttat tcacgaaagc ggcgatttag acccgcttgt catcatggcg gcggcacatt
     1441 accaatttga agccatccac ccgtttacgg acggcaacgg gcggacggga cgcatattga
     1501 acagcctgct attgattgaa aaagggcttt tggatttgcc tattttgtat ttgagccgct
     1561 acatcatcga aaacagggcg gactattacc gcctgctttt aggcgtaacc gaacggcagg
     1621 attgggaaag ctggataatc tacatcttag acggcgtagc cgacaccgcc gattggacgg
     1681 tatcgaaaat agatgcgata cgctgcctgt tcgagcagac acgtcaacac atacggacac
     1741 acgcacaagg aatctacacg cacgaactgg taaatcttct gtttgagcag ccatatacac
     1801 gcattgccaa cctagaagcg gcagggatag ccaaacggca gacggcctct aagtacctga
     1861 aagagctttc aggcataggt gtgctgcaag aaatcgccat cggcagggac aaactattca
     1921 ttcatccgcg cctaatggaa ctattgcggg gagagggcaa cagctttact tcattctaac
     1981 cccctcttca tccctacatg actaacacga aacagggatt ttgacacccg aaccggtacc
     2041 ccttgtattt tccccgcgaa aagccggcat ccgcccgcgc atcatgggag caacaaaacc
     2101 cctgcctaaa attttgactt gtgcaaatta ggggtatatt tggggggtat attgaaaaat
     2161 ggctaaaata aaatgtttaa taaacaaaat gttgaaactt aatttcgata gagcctctgc
     2221 atatcgtatt gaggcgttaa tggaatttga gaaagctatt tttaaataag aaaaggtaac
     2281 tattaaatag ctacctttct aaaattaaat atcaacacat cgttaaaaca cagccaaaaa
     2341 aaaaccgaaa ccgaacggtc cggattcccg cctgcgcggg aatgacggat ttcaagatta
     2401 cggtattgtc ggggatgatg ggaaacggtg ggaattgtgt aaaaaatgcc gtctgaagcc
     2461 tttcaggcgg catttgcggc attcggacat ttaaaaatcc cgtcattccc gcgcaggagg
     2521 gaatccgaat acatccgcac agaaacctgc atcccgtcat tcccgcgaaa gcgggaatct
     2581 agaacgtaaa atctaaagaa accgttttat ccgacaagtt tccgcaccga caggtctaga
     2641 ttcccacttt cgcgggaatg acgggatttt aggtctctgt aatctcgggc agctttccgt
     2701 tatcgtttta acccaaagac accatttcaa tctgctccat cgtcctgccc agaaaacgct
     2761 cgccgatggt tctgaatttc aaaggaatat cgctgacgta aaaacggtag tcgggattgt
     2821 tgttgccggt atcgagcaat ccttcctgtg caaggacgcg tgcggtttct tcggccgtcg
     2881 taatcgcgga atcgaccagc gcgacattgt gcgcctccct gccgattaag ggcttgagca
     2941 agggaaagtg cgtgcagccc aacaccagcg tatcgatgcc gtctgcaagc aatggtttga
     3001 ggtattcgca tacggtcagg cgggtaactt cgtgttccaa ccaaccttcc tccaccaaag
     3061 ggacgagcag cggcgcggcc tgcgtgcgga cgagcgtgtc ggggttgtcc ctgtggatgg
     3121 cgcgcgcata agcattgctg ttgactgtcg tattggtggc gatgatgccg attttattgt
     3181 tgcgcgtcgt tgccagcgcg gctttggcgc cggcggaaat cacgtccaaa acgggcatat
     3241 tgcccgtctt ttgacggatt ttccgccccg caaccgccgc aatcgtgttg cacgcgataa
     3301 ccatcgcctt gacatcgtgt cccaataaaa aatcgacaat ctgcatcgag aaattttcga
     3361 tggtcgcctt agatttcgtc ccgtaaggca cgcgcgccgt gtcgccgaaa taaatgatgt
     3421 tctccatcgg cagccgttcc atcagcgcgc gcacattggt caaaccgccg attcccgagt
     3481 caaaaacgcc gatgggccgc tgcctgccga tattttccat cctttttcca atccgtccta
     3541 aacatacaat ccgcatattg tacacgcgcc gctttttctt gacagccatg ccgtctgaaa
     3601 gcaggatgcg tatttggctg ttataatgta cggcaatgcc gccgccgggc gcacggtttt
     3661 ccgaccgcct gccctccgat ccgccacata aaagttgtaa agcccctatg tctgattttt
     3721 cccccgtttc ccgattcctt gccgacgaag ccgccacact cgatttgggc gcggcgtggt
     3781 cttcccgttt aaacgcaccg ctggtcattt acctcgaagg ggatttgggt gcgggcaaaa
     3841 ccacgctgac gcgcggcatc ctgcgcggat tgggtcacca gggcgcagtc aaaagcccga
     3901 cctacgccat cgtcgaatct tatccgctgg aacgcttcgc cctgcaccat ttcgacctct
     3961 accgcttctc gttccccgaa gaatgggaag acgcggggct tgacgagctg tttgccgcaa
     4021 acagcgtctg cctgatcgaa tggccgcaac agggaggcga atttaccccg cctgccgaca
     4081 tcaccgcaac attgacacac ggcggcggcg gcagaaaatg cctgctgacc gcccataccg
     4141 aacgaggacg cgaaagcctg ccgctatgac caaactgaca cgaagacaaa tcatccgccg
     4201 caccgccggc acactgttcg ccctgagccc catcgcatcc gccgttgcca aaacggtacg
     4261 cgccccgcag tttaccgccg cacggatatg gccgtcgcac acctacaccc gcctgacgct
     4321 ggaaagcacc gccgcgctca aataccagca cttcgcgctc gacaacccgg gcaggttggt
     4381 cgtcgatata caaaacgcaa acatcaatac cgtattgcac gggctttccc aaaaagttat
     4441 ggcggacgac ccctttatcc gcagcatacg cgcgggtcag aacacgccga ccaccgtccg
     4501 cctcgtcatc gacctgaaac agcccaccca cgcacaagtc ttcgcgcttc cgcccgtcgg
     4561 cggctttaag gaccgcctcg tcgtcgacct ctatccgcac gggatggatg ccgacgatcc
     4621 gatgatggcg ctgctcaacg gcagcctgaa caaaaccctg cgcggctctc cggaagccga
     4681 ccccgcccaa aacaccacgc cccgacccgg gcggggcaaa aacgggcgca gacccgtcat
     4741 catgctcgat ccgggacacg gcggtgaaga ccccggcgcc gtcagcccgg gcggtttgca
     4801 ggaaaaacac gtcgtcctct ccatcgcccg cgaaaccaaa aaacaattgg aagccttggg
     4861 ttacaacgtc tttatgacgc gcaacgaaga cgtgttcatc ccattgggcg tgcgtgtcgc
     4921 caaagggcgc gcacggcggg cggacgtatt cgtctccatc cacgccgacg ctttcaccag
     4981 cccctccgcg cgcggaacgg gggtttatat gctgaacacc aaaggcgcga ccagctctgc
     5041 cgccaaattc ttggaacaaa cccaaaataa tgccgacgcg gtcggcggcg taccgaccag
     5101 cggcaaccgc aacgtcgata ccgccctgct cgacatgacg caaaccgcca cactgcgcga
     5161 cagccgcaaa ctcggcaaac tggtgcttga agaattgggc aggctcaacc atctgcacaa
     5221 aggcagggtg gacgaagcca atttcgccgt tttgcgcgca cccgatatgc cgtctatcct
     5281 ggtcgaaacc gccttcctgt ccaatcctgc cgaagagaag ctgctgggca gcgaatcctt
     5341 ccgtcggcag tgcgcccaat ccattgcctc gggtgtccaa cgctacatca atacatccgt
     5401 attgaagcgg ggttgattga aaaggcggtg tttcaaaata tagttgtcgg atgaaggcag
     5461 gcataaacct acttccgccc caaaccgcgt ttttatgatg aaaggtatgg ataaattgag
     5521 ataccaacgg gattttttaa atatccgccc catttttaca gccggcgaac aagaatacct
     5581 gaccgaattg tccgatcggc ttcctttatc tgttttaacg gactccgtgc gtaatattga
     5641 agaaatcggt atcgattttg tgtacagtcc tgccaaattg gaaggaaaca cttataatca
     5701 atacgatacg caggcattgc ttaaactcgg gcaaaccgca ggcgggaagt tatattcgga
     5761 tgccgtaatg ttaatcaacc tgagggaaag ttaccggcac ctgctgtccg gtttggacag
     5821 ccccaagccg tttgactggt tggatttctt aaaaaccacc cacagcctga tttcggaaaa
     5881 cttactggaa aaaggctccg gcggcgtagt ccgccgcgac agcgttacta taagcgggac
     5941 agactacacc cctttatcca acccgcaaag tttggatacg gaattgaaat ggttgcttca
     6001 agaagccccc aaaatagaaa atcctttcga ccgcgccgtt tatctccata ataatttggc
     6061 gtatctccga tattttaagg actgcaataa acgtactgcc agaaactgta tgaccttgtc
     6121 gctgatgcgc tccggttttt ttccttgcgt attctccccc gacagttatc ccgcctatgc
     6181 cgaggctgtc gtagcctatt acgaaaccgg cgattacggt ttattcaaga aatattttat
     6241 ttcggcatac gaaaataccg taaataagta cggtccccag cctgatgtgg atattttccg
     6301 aaatttctcc atttaaaaag ccgtctgaac atttttcaga cggcattttt gtacactttc
     6361 ctcaacggtt cgatccctgc accatatcga tcaggaacag gcggcagtcg atgttgccgt
     6421 tataaagcgg gattttccgc ttgggcagca ggcgcatgaa tttgggcatt tccctgtcgc
     6481 cggtaaacat gccgacccgc cagccggcat agtgtttttt caaccacgta cccaactgcg
     6541 gataaagtgc ctgcaaagcg cggacttcct caaggcgcac gccgtagggc ggattggaca
     6601 ccatgatgcc gttttcgccg ttcggacgga cggactgcgc gtcggcaacg ctgaaggaaa
     6661 cgatgtcgtc cgctccggca ttgtgtgcgt tgtccaatgc cgtctgaacg atgcggcggc
     6721 cgttgtcgct gcctgcaatc ggggcgcgga cggggcgggt ttgcgcttcg gcgcggcggc
     6781 gcaaatccgc ccacagcgtt ttatcgaaat tttgcagttt ttcaaaaccg aagcggcgca
     6841 tcatacccgg cgcgcggccg gcggcaatcc aagcggcttc gatagcaatc gtgccgctgc
     6901 cgcaaaacgg gtcttgaaac ggctgcgtgc cgtcgtagcc tgccgagagc agcagcccgg
     6961 cggcaaggtt ttcgcgcagc ggggcttcgc cggtatccag gcggtagccg cgtttgaaca
     7021 gggcttcgcc cgaagtgtcg atgaagattt cgacattgcg ttcgtccaaa aaggcgtgga
     7081 tgcggacatc gggcgcggct ttgtccacgc tcggacgtgc gtcgtaaatg tcgcggaaag
     7141 cgtcgcagac ggcatctttg acggtcaggc cggcaaattg gaggcttttg acgttggcgc
     7201 gttttgcctc gattttgact ttgaacgtct gctgcaaggt aaaccagttg aaccagttga
     7261 tgtttttggc aagtttgtag atgtcgcgct cggttcggta tgtgcctttg gtcaggcgca
     7321 gcaggatgcg gccggcggtg cgcgagtgca ggttggcggc gtaggcctgt tccaatccgc
     7381 cacggcagga aacgccgccg tcaaacgccc gaacctcggt gcagccgagg ctttcgagtt
     7441 cttgcgataa aacggtttcc aagccgcgcg ggcaggtggc gaaaagtgta tataaagtat
     7501 tcatagcgtg tcctttccgg acggatgttc cggcaatggc ggattgaaag gtttattcgg
     7561 cggtaaatgc cgtctgaaat gtccggctgc cgattgtaat gtccgccgta aaatcgcgcc
     7621 tgccttcgac acagacgggc aggcggatgc gtgctgcctg ccaattcccc gacggttgcc
     7681 gctcgaacat atagcggttg aaacccatat ccatattttt catactgaag ctgatgctga
     7741 cctgttccgt gccggcgggc gcgtgttcga tataaatatc aaacggtttt ttggttgaaa
     7801 cggcggcggc gcggacacgg cttccgtccg gcagcgtgca accctcggtc aaatcgcatt
     7861 gcgccgccac ggcttgcggc tgctgcgcct gccaccattg caacaaaacg agcttgaagg
     7921 cggcaaaggc agtcagcagc agtacggcaa gcagtaattt gcggttttta ttcatgattc
     7981 ttccctattg tttcatccct taccaccgcg tgcgccccca atccgagcca tagttttcca
     8041 taacgtgcaa ccgtctgtcc gtttgctgca agtacgagcg gcacggatac gccctgccgc
     8101 agggcttctg ccgcctcttg tgcctgaaca tcatcgcaca cgcgccacac gaccacatcc
     8161 gcgccctttt cctgcgcctg ccgccattgt tcgcgggtct gcaccatcat ccacacgctg
     8221 ccggcgtgtt cggacctgtc ctgccattgt gccgcctcca tcaaaacgtt cgcaccgctt
     8281 ccccctgccg aacccaaagg caggacgcgg tacgcgccca tactgttttc gccgtacaaa
     8341 cctgttttca ggctgccgta caaacggcgc gggacggaca gcgaacgcaa aagcgcaccg
     8401 ttggcaggca gcatgggggc aacggtaaaa tcacccgcct tctgccaaga ccattcctgc
     8461 ccttcgcgcg attgcggtcc gccctcccat tgatcggggt ttacccataa gaatttcagg
     8521 cagacgcggg cgtgttcgta ggaatggatt ttggtcagcc aaggcgtggc ggcgaggatg
     8581 cggatgccga gttcttctcc aaactcgcgt tgcagggctt ggaagtcggt ttcgcccgcc
     8641 tcgaccttgc cgccggcaaa ttcccaatat ccggcatagg gcttgccttc ggggcgcgag
     8701 ccgagcaggt aattgccgtc tgaatcgagc aggatgccgg caacgacgcg gataaggggg
     8761 cgggtatctt ggatcatggc gggtatgcgg ttggtcggtc ggaatgcaaa tgagggaagg
     8821 gcaatgccgt caacgggttt gccgaatttc agacggcatt atttttcggc aacggcaaac
     8881 gccaacacgg tatcgccttc gtccgccata ctcaggctga cgcggctgat gccttgttct
     8941 tccagccatt cggacagggc ggggccgtag aaaaattcgg gcttgcccaa tgcgtcgtgc
     9001 ccgatgccga tgttgcagaa ggaaaccgcg cctcgtatgc ccgtgccgac ggctttggcg
     9061 aaggcttctt tggcggcaaa gcgtttggca aggtagttga cgggtttgcc cgcctgcgga
     9121 aattcaagca gctcttcggg agtgagtatg cgccctgcaa acgcctgtcc gaattttttg
     9181 tttaagcgga tgatgcgctt gagggaaacg atgtctgtac cgatgccgta aatcatattt
     9241 gcgctccttt ttcgtgaaat gccgtctgaa acggtttgcc tgcccttacg gcagcagcct
     9301 tgccctgaac atcgcctcct tcatctggcg cacggcttcg ggaagtccga ggaagagggc
     9361 ttgggcaatc agcgaatgcc cgatgttcag ttcgcggatg gcgaggattt gggcgacggg
     9421 ggtaacgttg tgtatggtca gtccgtgtcc ggcgttgacg accaagccca aatcgccggc
     9481 gaaatgcgcg ccgttttgga tgcgctcgaa ctgcctgatt tgttcggcgt ggctttgtgc
     9541 gtcggcatat gcgccggtgt gcagctcgac aacgggcgcg ccgacatcat aggcggcttg
     9601 gatttgcctg tcgtcggcat cgataaacaa ggacacgcgt atgcccgcgt cggtcaggat
     9661 tttggcgaat tcggcgattt tttcctgttg cgccaatacg tccaaaccgc cttcggtcgt
     9721 gatttcctgc cgtttttcag gcacgatgca cacgtcttcc ggcatcactt taagcgcgtt
     9781 ttcgagcatt tcttccgtca acgccatttc aaggttcagg cgcgtgcgga tggcgttttt
     9841 gacggcaaat acatccgcgt ctttgatgtg gcggcggtct tcgcgcaggt gcatggtaat
     9901 caggtctgca ccgtgcgttt cggcaaccag tgccgcctcc acggggctgg gataagtcgt
     9961 gccgcgcgcg ttgcggacgg tggcgatgtg gtcgatgttg acacccagaa gcataatgtt
    10021 tccttttctc ttcgttttcc tgtttgacgg catggtgcgg gcgcagctac gcggtattgc
    10081 ggtcgaactg ccttatctgt tccaacacct gccgcgattt caagccttcg ggaagcagcg
    10141 tgccgattag cagccgtgtg attttcaatg cctgttgcag gctttcggcg gtgcggaaac
    10201 tgccttcgcg caaatcgatc aggctctgcc cttcgacaat tgcgccggct tcgtgcgaaa
    10261 gcgcgcctgt gtcccggcat acgggcatga ccgcctcttc gggcatcagc cggtaggttt
    10321 tgtccgccaa aatatcgccg cccgtgccgt cggcgtgcaa atcgggggca acgcccaagg
    10381 cattgagcag cttccattcg aagcggcgca aatcggcaat atggttggct tcacggcaca
    10441 ctgcctccat cacttttgcc aacgcgtcgt acagttcgga catcgggtct tcgcgcgcgg
    10501 tcagtttcaa caccagctcg ttcacataca gaccgctgaa caacgccctg ccctgcggct
    10561 gccgccatcc gcccatccat tcggcgcggt gcagggtttt gagttcctga ctgccgtacc
    10621 acgacacgct ggcagggacg aacggaacca atacgccgcg cagctcgctc tgccgtttgc
    10681 gcgcgctgcg cgccagcaaa gccacacgcc cgtaacggcg gctgaatgct tcaacccgca
    10741 ggctgctttc gcgccaaggc gaagatgcca gcataaaaac gggttcgtgg ttgactcggt
    10801 attcggacat aatcgttgtg gaaatgccgt ctgaacgctg ccgcgactac aaaacggcag
    10861 tcggatacga accgatggct ttgacaaacg aagcgcgttc gcccaagcgt tccaatgccg
    10921 tctgaatctg cgcgtcccgg cggtgtcctt cgatgtcgat gaagaacagg tattcccaca
    10981 aaacggattt gctcggacgg ctctcaaact tggtcatgga aatacccgat tcggtcagcg
    11041 gttgcagcag cgaggcaacc gcgccggcgc ggttgggcgc ggaaacggcc agcgaggttt
    11101 tgtcgctgcc gcttgcaccg gtttcgtgat gtcccatgac cagaaagcgc gtggtgttgt
    11161 tcggttcgtc ttcgatgcac tcggcaacca tatcgagtcc gtaaatttcc gccgccgtgc
    11221 gtccggcgat ggcggcaacc gtaccgtcgt ccgattcggc aaccagcctt gcggcttcgg
    11281 cattgctgga cacggcaatc cgttcggcat tgggcagacg cctgcccaac cagtcgttgc
    11341 actgcgccaa cgcctgcgcg tgcgaaaata ctttggcgat accctcggtg ctgccgttgt
    11401 ttttgcgcaa caggttgtgg tggatgcgca aaacgacttc gccgcacgcc tgcaacgcgg
    11461 taacggcaag caaatccaat gtgcgcccga ccgagccttc ggtcgagttt tccacgggcg
    11521 cgaccagata atccgcctga cgggtttcaa cctgtttgaa gcagtcgtct atggtcggac
    11581 acgccattgt atgcgcggcg tgtccgaaat gcttgattgc cgcctgttgg gtaaacgtgc
    11641 cctgcggccc cagataggca atggtcagcg gacgttcgac ggcgaggcac tcgctcatca
    11701 cttcccgaaa caggcgtgct accgattcgt cgggcagcgg gcctttgttc aaatcctgaa
    11761 tgcggcgcaa cacggcaacc tcgcgctccg ggcggtatac cgcgcccgtg cctttcagct
    11821 cgccgatggc gtgcgcgtgt tgcgcgcgtt cgttgagcag gcgcaggatt tccgcatcga
    11881 tggtgtcgat ggcgttgcgg tggggaagga ggagttcgtc gatagtttgg gacataattt
    11941 cgcctgaacg gtaatttgcc gtgcattcta acatcaaatg ccgtctgaag cggtttcaga
    12001 cggcattttc cgttaaaggt attcgctttg cccgttttct ttccacgcac gatgcgagca
    12061 gagccacaaa agcgcaatgc cgcaagaggc ggacgcggtc atggttgccg ccatcacggt
    12121 tgccgccatg accacgcccg cgccgattaa ggaccggaat acacccgaca cggcgttcgc
    12181 gctgccgccc tcttctttga aataagacat aaagcatgcc tgcgtgtccg caccgaccag
    12241 gccctgcgta ccgacggaaa acatcacgca cgcgaccggc agccaaaacg ggggcaaccc
    12301 gaaaaacagc acggcggcga gttgggacgg gttggcggca aattggacga caatcccccg
    12361 cagcaggatg ctttgcggat gcgcgccggt tttaagccgc cacgcggtaa cgcggctgaa
    12421 aaacatcatc gtgatgatgt tgagtgcaaa cacccatgcg taccggtgcg gcgtaacgtg
    12481 gtagagctgc cggtacacga aggaagattc ggtcagaaag gcgaacatcg aaccgaagct
    12541 gaatgcctga aaaaacagat aacccatcgc ggcacgggtt ttcaatacgc gcttgaaccg
    12601 ccccgccacc agcccgaaca catccctgcc gattttgccg ccgacggcgg gattgggcag
    12661 gaaatactgt accaaaccgg gcagcaccgg cgaatacgcc gccaagaaaa cgaaaatcgc
    12721 ccgccatccg cccaatccct gcaacaatgc gccgaccatg ggtgcggcca gcggcacaac
    12781 catcagaatg atgccgataa gggcaaacat ctgcgcggct ttgcgtccgg aataataatc
    12841 gcgcaccatc gcaccgacga tgactacagc catgcctgcg ccgaacgcct gtaccgcacg
    12901 caggttaagg agctgttcgg tactcgaagc aaatacgatg gcggcaacgg caaggcaata
    12961 tacaatcaaa ccggtcaggg cgacgggttt gcgccctttg atgtcggaca ccgcgccgcc
    13021 ggccacttgc ccgaacgccg tgccgaacat aaacaaactc agactctatt cgatacggtg
    13081 gatatccgcg ttcagcggct gcgccatttc gggaatcgcg ggcaggtagg catcggtgga
    13141 aaacggcatc agcgcgacca gtaccgccat caaaaccgcc atcagttttc cgctcatcgg
    13201 aggaagggtc ggagacatgg tttttccgtt caaatcaaat aaagaaaccg tcgcaaacga
    13261 cggtcggcac gggcgcaagt ttaacatatc ggacggcggg gcaaaaccga aggatatgcc
    13321 gcccgaaacc gcttcagacg gcattgccgt tttgcgcgtc cctgcgcgac agccggccgg
    13381 cggcgaaagt ggcggcgatg acgacaaaca cgcccaaggc ggacacgggc gataaatgtt
    13441 cgcccaaaat caaaaccgcc aacagcacgc cgacgacggg ttcgagcgaa atcaacagtc
    13501 ccgacgcgtt ggcaggaaca cggctcatcc ccttgttcca cagccaatag gcgtaccagc
    13561 cgcaccccaa acccaaatac aacagcgaca ataccatccc gacgctccag tccacggtat
    13621 aactttgcgc caaagcaagc gaaaacggca ggcacatcaa cgatgcggcg gcaatggaaa
    13681 cagatgtgaa tgccggtgcg ccgatgcggg caatcagcct ttgcgtcgga cgcatagcgg
    13741 cacaaaagcc cgcgcccgcc aacaacacca gcaggcagcc gaaccagccg acttcgccgc
    13801 cctcttccgc accgcccgcc atcagcagcg cgacaccggc aaatgccgcc gcgccgcata
    13861 tccagtggta ggcacgcgct ttgtcgttga agaaaaagtg tccgacaaac accatcagca
    13921 gcggctcgag tccgacaatg accgatgcgc tggcggcgga agtgtatttc aacccgacaa
    13981 actgaagcag cagggtcagc acatagttga cgaacgacac aatcagcaac ggcttccatt
    14041 cctcacgcgg aatcttgccg acatgacggc ggcaggcggg cagtgcaggc agcgcggcaa
    14101 tcagcaggcg cacgccgacc atcaatgcgg gatcgatgcc gccatagaca tatttggcgg
    14161 caataaacga gctgctccag ataatcaggg caaggatttg gtaaaacata gtgagtggta
    14221 aagtcagatg gtcatacaaa agtatagcgg attaacaaaa atcaggacaa
//
LOCUS       DAWTQS010000055        13357 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.055, whole genome shotgun sequence.
ACCESSION   DAWTQS010000055 DAWTQS010000000
VERSION     DAWTQS010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13357
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.055"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            289..579
                     /locus_tag="WNA25_001610"
     CDS             289..579
                     /locus_tag="WNA25_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFA9824330.1"
                     /translation="MTIRPLHDRVVVKRLEAEEKTASGIVLPGAAAEKPDMGEVIAVG
                     AGKIGKDGARRPLDVKAGDKIIFGKYSGQTVKADGEELLVMREEDIFGIVEK"
     gene            672..2306
                     /gene="groL"
                     /locus_tag="WNA25_001611"
     CDS             672..2306
                     /gene="groL"
                     /locus_tag="WNA25_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271863.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFA9824331.1"
                     /translation="MAAKDVQFGNEVRQKMVNGANILANAVRVTLGPKGRNVVVDRAF
                     GGPHITKDGVTVAKEIELKDKFENMGAQMVKEVASKTNDVAGDGTTTATVLAQSIVAE
                     GMKYVTAGMNPTDLKRGIDKAVAALVEELKNIAKPCDTSKEIAQVGSISANSDEQVGA
                     IIAEAMEKVGKEGVITVEDGKSLENELDVVEGMQFDRGYLSPYFINDAEKQIAGLDNP
                     FVLLFDKKISNIRDLLPVLEQVAKASRPLLIIAEDVEGEALATLVVNNIRGILKTVAV
                     KAPGFGDRRKAMLQDIAILTGGVVISEEVGLSLEKATLDDLGQAKRIEIGKENTTVID
                     GFGDAAQIEARVAEIRQQIETATSDYDKEKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KDRVEDALHATRAAVEEGVVAGGGVALLRARAALENLHTGNADQDAGVQIVLRAVESP
                     LRQIVANAGGEPSVVVNKVLEGKGNYGYNAGSGEYGDMIGMGVLDPAKVTRSALQHAA
                     SIAGLMLTTDCMIAEIPEEKPAVPDMGGMGGMGGMM"
     gene            3010..4545
                     /locus_tag="WNA25_001612"
     CDS             3010..4545
                     /locus_tag="WNA25_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HFA9824332.1"
                     /translation="MSDSKTKERATFGTRRAFMIAAIGSAVGLGNIWRFPYIAFENGG
                     GAFILPYLVALLTAGIPLLLLDYAIGHRYRGSAPLAFRRLGRWFEPVGWWNVMTNIVI
                     CIYYAVIIGWAASYTYYSVNAAWGADPQGFFFKDFLQMAGPEALGLDFVGKVAGPLAG
                     VWVFTAAIMALGVQKGVARASSFFMPLLLVMFLIMVGISLTLPGAAKGLDALFTPDWS
                     KLADSKVWVAAYGQIFFSLSICFGIMVTYSSYLKKKTDLGGTGLVVGFANSSFELLAG
                     IGVFAALGFMAQAGGKAVNEVASGGIGLAFIAFPTIINQAPMGWLIGILFFGSLVFAG
                     VTSMISILEVIVAAIQDKLNIGRVNATLLVCIPMGIVSTLLFGTATGLPVLDVMDKFV
                     NTYGIVAAGFVYVAAIIIGGRLPELRRHLNALSSIRVGGLWTACVVFTVVMLGYMLYQ
                     DTAGLLEKNYGDYPDGFLNIFGWGMSAALIMFGLLLSLLPWKHGQDFNVKDEHEHEQG
                     GEK"
     gene            4542..4634
                     /locus_tag="WNA25_001613"
     CDS             4542..4634
                     /locus_tag="WNA25_001613"
                     /inference="COORDINATES: protein motif:HMM:NF033493.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HFA9824333.1"
                     /translation="MSTSAIVMMVAAIAVIWGGLLLSLLRLPEE"
     gene            complement(4670..5110)
                     /locus_tag="WNA25_001614"
     CDS             complement(4670..5110)
                     /locus_tag="WNA25_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824334.1"
                     /translation="MKHAPIILLLTASSAALLSAGCTALPSDRPPLRTLPKSAPIQPA
                     NPYSRPPSGTPDGAFSTRASNGRVLKSIVKNGVFDRFVDIYHPNGKLHSHTPVENGVA
                     QGYTEQGILRTRILYRGTGISSERKLWMRRARWNANGSRKTNAV"
     gene            complement(5304..6524)
                     /gene="lysA"
                     /locus_tag="WNA25_001615"
     CDS             complement(5304..6524)
                     /gene="lysA"
                     /locus_tag="WNA25_001615"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFA9824335.1"
                     /translation="MTLFCEQVAYPRLAEEFGTPLYVYSQSALTGAFENYQTAFAALN
                     PLVCYAVKANGNLSIIKHFASLGSGFDIVSGGELARVLAAGGDAAKTIFSGVGKSEAE
                     IEFALNAGVKCFNMESIPEIDRIQKIAARLGKTAPVSLRVNPDVDAKTHPYISTGLKA
                     NKFGIAYADALEAYRHAAQQPNLKIIGIDCHIGSQLTDLSPLVEACERILILVDALAA
                     EGIVLEHLDLGGGVGIVYKDEGVPDLGAYARAVQKLMGTRRLKLILEPGRSLVGNAGA
                     LLTRVEFVKHGEEKNFVMVDAAMNDLMRPALYDAYHHIEAVETKNIEPLTANIVGPIC
                     ETGDFLGKDRTIACEEGDLLLIRSAGAYGASMASNYNTRNRAAEVLVDGGGYKLIRRR
                     ETLEQQMANELACL"
     gene            complement(6535..6705)
                     /locus_tag="WNA25_001616"
     CDS             complement(6535..6705)
                     /locus_tag="WNA25_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFA9824336.1"
                     /translation="MKYGVFFAAATALLLSACGYKGGLYLPKEGDTARFGAIQTGLQL
                     QGKPQSAPPTQK"
     gene            6776..7099
                     /gene="cyaY"
                     /locus_tag="WNA25_001617"
     CDS             6776..7099
                     /gene="cyaY"
                     /locus_tag="WNA25_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFA9824337.1"
                     /translation="MMTESEFIRASEALFEHIEDQIDENGWDFDCRFAGNVLTIEAGD
                     GTQIIVNRHTPNQELWIAAKSGGYHFAEQNGKWLATRDSRDFYDVLNEALSAASGEAV
                     EIAEL"
     gene            7132..7551
                     /locus_tag="WNA25_001618"
     CDS             7132..7551
                     /locus_tag="WNA25_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HFA9824338.1"
                     /translation="MAQLPLYRTAEIGNFTVGTPKVLESFSKHIPYGVVFEDDGNTGY
                     FYAASQEGILDALHIYNVEDVSDKHIPNHVLILWDGACTIAALCINDYIHAVYDFVEQ
                     AGYCRNGFPEAGGEWVKVENRVLDDELLDKILSRKPT"
     gene            7573..8079
                     /gene="luxS"
                     /locus_tag="WNA25_001619"
     CDS             7573..8079
                     /gene="luxS"
                     /locus_tag="WNA25_001619"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFA9824339.1"
                     /translation="MPLLDSFKVDHTRMHAPAVRVAKTMTTPKGDTITVFDLRFCIPN
                     KEILPEKGIHTLEHLFAGFMRDHLNGAGVEIIDISPMGCRTGFYMSLIGTPSEQQVAD
                     AWLASMQDVLNVKDQSKIPELNEYQCGTYLMHSLAEAQQIAQNVLARKVAVNRNGDLA
                     LDESLLNA"
     gene            8223..11015
                     /gene="polA"
                     /locus_tag="WNA25_001620"
     CDS             8223..11015
                     /gene="polA"
                     /locus_tag="WNA25_001620"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFA9824340.1"
                     /translation="MSNRPTLLLVDGSSYLYRAYHAMGQNLTAPDGAPTGALYGVLNM
                     LRRLRSEYPHDYCAVVFDAKGKNFRHQMFEEYKATRPPMPDDLRPQAEALPDLVRLTG
                     WPVLVIGQVEADDVIGTLAKQGAEHGLRVIVSTGDKDMAQLVDERVTLVNTMSGETLD
                     IEGVKAKFGVRPDQIRDYLALIGDKVDNVPGVEKCGPKTAVKWLEAYGSLQGVMEHAP
                     EIKGKVGENLQAALPQLPLSYDLVTIKTDVDLHTELSDGIESLRRTAPKWAQLAVDFK
                     RWGFRTWLKEAESNMNTGSTDDLFGSDSIGEQAALNAEIPSEKRAEKATAPEKLDYQA
                     VTTEAQFAALLDKLAKADTIGIDTETTSLDAMNAELVGISIAFQAGEAVYIPVGHSLT
                     AAPEQLDLQDVLGRLKPHLENPALKKIGQNLKYDQHVFANYGIALNNIAGDAMLASYI
                     IESHLGHGLDELSGRWLGLETITYESLCGKGAKQISFADVAIGQATEYAAQDADFALR
                     LEARLRAQMDDKQLEMYEKMELPVAQVLFEMECNGVQIDRAELARQSAELGAELMKLE
                     QEAYAAAGQPFNLNSPKQLQEILFDKMGIPTKGLKKTAKGGISTNEAVLEQLAPDYPL
                     PKIILQNRSLAKLKSTYTDKLPEMISPRDNRVHTTYAQAVAITGRLASNNPNLQNIPI
                     RTAEGRRVRRAFTAPPGSVIVSADYSQIELRIMAHLSGDKTLIAAFQSGEDVHRRTAA
                     EVFGTAPENVSPEQRRYAKTINFGLIYGMGQYGLAKSLGIDNLSAKNFIDRYFARYPG
                     VAEYMQRTKEQAAAQGYVETLFGRRLYLPDIRNKNANARAGAERAAINAPMQGTASDL
                     IKRAMINVRNWLSDGIGSKLVMQVHDELVLEVVETELDFVKEKLPQIMAKVDGGLLDV
                     PLVAEVGVGENWEEAH"
     gene            11577..12272
                     /locus_tag="WNA25_001621"
     CDS             11577..12272
                     /locus_tag="WNA25_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFA9824341.1"
                     /translation="MFPDKYKLSLEENIFLAKKVLVAQIHNLSRFENCQTTLLQTEQI
                     INGKNVASASLEDIQTILNLKRAYQYVISHISNGEPVNISLLKRINNIVAKDDSLVPV
                     DFRTGSVGVTLLDGSRHAPNPVKEIEVARVLQNIGLQSGSTTEAAVRFMLYCMRQQVF
                     WDGNKRTATLFANGLMMAGGCGILEISEMQMPRFNEKLSAFYRTGDDTDISKFVYQNC
                     ISGIDYFGADEDI"
     gene            complement(12264..12374)
                     /locus_tag="WNA25_001622"
                     /pseudo
     CDS             complement(12264..12374)
                     /locus_tag="WNA25_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(12512..>13357)
                     /locus_tag="WNA25_001623"
     CDS             complement(12512..>13357)
                     /locus_tag="WNA25_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFA9824342.1"
                     /translation="YANSGLSEFSATLNSVFAVQDELDRVFAEDRRNAVWTSGIRDTK
                     HYRSQDFRAYRQQTDLRQIGMQKNLGSRRVGILFSHNRTENTFDDGIGNSARLAHGAV
                     FGQYGIGRFDIGISAGAGFSSGSLSDGIRGKIRRRVLHYGIQARYRAGFGGFGIEPHI
                     GATRYFVQKADYRYENVNIATPGLAFNRYRAGIKADYSFKPAQHISITPYLSLSYTDA
                     ASGKVRTRVNTAVLAQDFGKTRSAEWGVNAEIKGFTLSLHAAAAKGPQLEAQHSAGIK
                     LGYRW"
BASE COUNT         3165 a         3521 c         3648 g         3023 t
ORIGIN      
        1 ccgcaggcag tacagatagt acggaaccga ttcacttggt gcttcagcac cttagagaat
       61 cgttctcttt gagctaaggc ggggcaacgc cgtaccggtt taaatttagt ccactataaa
      121 aaacggcgga aataaatttt tttccgcctc acttgaattt tcccgcacac accctaattt
      181 tgccgacttg tacgggcagg ccgttaggcg gcagcccggt ttccacttca atttgtccga
      241 accgttcggg cagatgattg ttttcaaacc atttatcgga gcataaatat gaccatccgt
      301 cctttacacg accgcgtcgt cgtcaaacgc ttggaagctg aagaaaaaac cgcatcgggc
      361 atcgtcctgc cgggtgcggc cgccgaaaaa cccgatatgg gcgaagtcat cgccgtgggc
      421 gcgggtaaaa tcggcaaaga cggcgcgcgc cgtccgctgg atgtcaaagc cggcgacaaa
      481 atcattttcg gcaaatacag cggccaaacc gtaaaagccg acggcgaaga gctgttggta
      541 atgcgcgaag aagatatttt cggcatcgtt gaaaaataaa tacggacacg atgccgtctg
      601 aaacggcaaa ccgccttcag acggcataaa cggttttatc agacagtttt aatgattttt
      661 ggagaattga aatggcagca aaagacgtac aattcggcaa tgaagtccgc caaaaaatgg
      721 taaacggcgc gaacattctg gcaaacgccg tgcgcgtaac cttgggcccc aaaggccgca
      781 acgtagtggt tgaccgcgct ttcggcggcc cgcacatcac caaagacggc gttaccgtcg
      841 ccaaagaaat cgaactgaaa gacaagtttg aaaatatggg cgcgcaaatg gtgaaagaag
      901 tcgcgtccaa aaccaacgac gtagccggcg acggtacgac taccgccacc gtattggcac
      961 aatccatcgt tgccgaaggc atgaaatacg ttaccgccgg catgaacccg accgatctga
     1021 aacgcggcat cgacaaagcc gttgccgctt tggttgaaga gctgaaaaac atcgccaaac
     1081 cttgcgatac ttccaaagaa atcgcccaag tcggctcgat ttccgccaac tccgacgaac
     1141 aagtcggcgc gattatcgcc gaagcgatgg aaaaagtcgg caaagaaggc gtgattaccg
     1201 ttgaagacgg caaatctttg gaaaacgagc tggacgtggt tgaaggtatg cagttcgacc
     1261 gcggctacct gtccccttac tttatcaacg acgcggaaaa acaaatcgcc ggtctggaca
     1321 atccgtttgt tttgctgttc gacaaaaaaa tcagcaacat ccgcgacctg ctgcccgtgt
     1381 tggaacaagt ggcgaaagcc agccgcccgc tgttgattat cgctgaagac gtagaaggcg
     1441 aagccttggc gactttggtc gtgaacaaca tccgcggcat cctgaaaacc gttgccgtca
     1501 aagcccccgg cttcggcgac cgccgcaaag cgatgctgca agacatcgcc atcctgaccg
     1561 gcggcgtagt gatttccgaa gaagtcggcc tgtctttgga aaaagcgact ttggacgact
     1621 tgggtcaagc caaacgcatc gaaatcggta aagaaaacac taccgtcatc gacggcttcg
     1681 gcgacgcagc ccaaatcgaa gcgcgtgttg ccgaaatccg ccaacaaatc gaaaccgcga
     1741 ccagcgatta cgacaaagaa aaactgcaag agcgcgttgc caaactggca ggaggcgtgg
     1801 cagtgatcaa agtcggcgcg gcgaccgaag tcgaaatgaa agagaaaaaa gaccgcgtgg
     1861 aagacgcgct gcacgctacc cgcgcagccg ttgaagaagg cgtggttgca ggcggcggcg
     1921 tagccctgtt gcgcgcccgc gccgctttgg aaaacctgca caccggcaat gccgaccaag
     1981 acgcaggcgt acaaatcgta ttgcgcgccg ttgagtctcc gctgcgccaa atcgttgcca
     2041 acgcaggcgg cgaacccagc gtggtggtga acaaagtgtt ggaaggcaaa ggcaactacg
     2101 gttacaacgc aggctccggc gaatacggcg acatgatcgg aatgggcgta ctcgaccctg
     2161 ccaaagtaac ccgttccgcg ctgcaacacg ccgcgtctat cgccggtctg atgctgacga
     2221 ccgactgcat gattgccgaa atccctgaag aaaaaccggc tgtgcccgat atggggggaa
     2281 tgggcggtat gggcggcatg atgtaagcaa tgccgtctga aaccttcaga caacaagccg
     2341 cacggtcaac gccgtgcggt ttttttgcga ataagtgcgg ctaaggcgca aaccttaaaa
     2401 ttatagcgga ttaacaaaaa ccggtacgtc gttgccccgc cccggctcaa agggaacgat
     2461 tccctaaggc gcccaagcac cgggcgaacc ggttccgtac cattcgtact gcctgcggct
     2521 ggccgccttg tcctgatttt tgttaatccg ctatatttcc cgccatccca aaaacgaaga
     2581 gcggcaggaa tttatcggaa aaacagcacc tctccgccgt cattcccgcg aaagcgggaa
     2641 tctaggttcg tccggtttcg gttatttccg atagattcct gccgcgttgg gggtctggat
     2701 tcccgtctgc gcgggaatga cgggacttta ggtttctgtt tttgtttttc tgtttttgcg
     2761 ggaatgacgg ggaagttggc ggttgtttct tatggagtgg tttttgtatt tggataaaaa
     2821 atttttcgtt ttcaagcctt caccgcttgc catcggcgtt aaaatttttt acgataagca
     2881 catagattgt aaacaatcgg ccacaagccg gtttgttttt tcagaagaca ttatccctgt
     2941 cagacgctgt ttctatatat gtttgcctat aacggcttgt ttttaataaa taattcaaga
     3001 ggtaccaacg tgtctgattc caaaacgaaa gaacgcgcca cattcggcac gcgccgcgcg
     3061 tttatgattg ccgccatcgg gtccgccgtc ggcttgggca atatttggcg tttcccctat
     3121 attgcttttg aaaacggcgg cggcgcattc atcctgccct atctggtagc gcttctgacg
     3181 gcgggcatcc cgctgctgct gctcgattat gccatcggcc accgttaccg gggttctgcg
     3241 cccttggctt tccgccgcct cggacgctgg ttcgagccgg tcggctggtg gaacgtgatg
     3301 accaatatcg tcatctgcat ctattacgca gtgattatcg gttgggcggc aagctatacc
     3361 tattattcgg tcaacgccgc ctggggtgcg gatccgcagg gttttttctt taaggacttc
     3421 ctgcaaatgg cgggcccgga agccttgggt ttggattttg tcggcaaagt cgccggccct
     3481 ttggcgggcg tgtgggtttt taccgccgcc attatggctt tgggcgtgca aaagggcgtg
     3541 gcgcgcgcct cgtcgttctt tatgccgctg cttttggtga tgtttttgat tatggtcggc
     3601 atttcgctga cgctgccggg tgcggcaaag ggcttggacg cattgtttac gcccgactgg
     3661 tcgaaactcg ccgattccaa ggtctgggtg gcggcatacg ggcagatttt cttttcgctt
     3721 tccatctgct tcggcattat ggttacctat tcttcttatt tgaagaaaaa aaccgacttg
     3781 ggcggaacgg ggctggtggt cggttttgcc aacagcagct tcgaactgct cgcgggcatc
     3841 ggcgtgtttg ccgcattggg ctttatggcg caggcgggcg gcaaggcggt caacgaggtt
     3901 gcctccggcg gcatcggttt ggcgtttatc gcctttccga ccattatcaa ccaggcgccg
     3961 atgggctggc tgatcggcat attgtttttc ggttcgctgg tgttcgccgg cgttacgtcg
     4021 atgatttcca tccttgaagt gattgtggcg gcgattcagg acaagctgaa catcgggcgc
     4081 gtcaacgcca cgctgctggt ctgcattccg atgggcattg tttccacgct gctgttcggt
     4141 acggcgacgg ggctgcctgt tttggacgtg atggacaaat tcgtcaacac ctacggcatt
     4201 gttgccgccg gctttgttta tgttgccgcc atcatcatcg gcggcaggtt gccggaatta
     4261 cgcaggcacc tgaacgcctt gtcctccatc cgcgtcggcg gcttgtggac ggcctgcgtc
     4321 gtgtttaccg tcgtgatgct cggctatatg ctgtatcagg acaccgccgg attgttggaa
     4381 aaaaactacg gcgattatcc ggatggtttc ctcaatattt tcggctgggg gatgtcggcg
     4441 gcgttaatca tgttcgggct gctgctgtcg ctgctgcctt ggaaacacgg tcaggatttt
     4501 aatgtcaaag acgaacacga acatgaacaa ggaggagaaa aatgagtact tccgccattg
     4561 tgatgatggt tgccgccatc gcggtgattt ggggcgggct gctgctttcc ctgttaaggc
     4621 tgccggaaga gtaagccttt agaacgttaa aaatgccgtc tgaaccgctt cagacggcat
     4681 ttgttttacg gctgccattc gcgttccacc ttgcccgacg catccaaagt ttgcgctcgg
     4741 acgatatgcc cgtcccccgg tacaggatgc gcgtccgcaa tatgccctgt tcggtatagc
     4801 cttgcgccac gccgttttca accggcgtgt gcgaatgcag tttgccgttt ggatggtaaa
     4861 tatcgacaaa gcggtcgaac acgccgtttt tgacgatgct tttcaagacg cgcccgtttg
     4921 aggcgcgggt gctgaatgcg ccgtccggcg tgcctgaagg cggccggctg tatgggttgg
     4981 cgggttggat gggcgcgctt ttcggcaggg tgcgaagcgg cggccggtca gagggaaggg
     5041 cggtgcagcc cgccgaaagt agggcggcgg acgaagcggt aagcaggagg ataatcggag
     5101 cgtgcttcat ttcaattcct tagatattgc accccataaa aggggcgcgt aatttttttc
     5161 aacaattccc ttcgtcagtc gggcgagacg gggatttgtt gcgtcgtcat ttgaaaacag
     5221 gctgtgtttg aatattccgc caagatgtca ctttaaacgg acaatgccgt ctgaaggctt
     5281 cagacggcat tttgatgttc ggcttatagg caggcgagtt cgtttgccat ttgctgttcc
     5341 aaggtttcgc gccggcggat gagtttgtat ccgccgccgt caaccaacac ctccgccgca
     5401 cggttgcgcg tgttgtaatt gctagccata ctggccccgt atgcgcccgc gctgcggata
     5461 agcagcaaat cgccttcttc gcaggcgatg gtgcggtctt tgccgaggaa gtcgccggtt
     5521 tcacaaatcg ggccgacgat gttggcggtc agaggctcaa tgtttttggt ttcaaccgct
     5581 tcgatgtggt ggtaggcatc gtatagggct gggcgcatca aatcgttcat cgccgcatcg
     5641 accatcacaa agtttttctc ttcaccgtgt ttgacaaatt cgacgcgcgt cagcaatgca
     5701 cctgcgttgc cgaccaagct gcggcctggc tcaagaatga gtttcagacg gcgtgtcccc
     5761 atcagttttt gaaccgctcg ggcatacgca cccaaatcgg ggacgccttc gtctttgtaa
     5821 acaatgccga cgccgccgcc taagtccaaa tgttccaaaa caatgccttc ggcggcaaga
     5881 gcgtcaacca aaatcaaaat gcgttcgcag gcttcgacca gtgggcttaa gtcggtcagt
     5941 tgcgaaccga tgtggcagtc gatgccgatg attttcaaat tgggctgttg tgcggcatgg
     6001 cggtaggctt cgagcgcgtc ggcgtaggcg atgccgaatt tgttggcttt cagacctgtg
     6061 gagatgtagg gatgggtttt tgcatcgaca tcgggattga cgcgcaggga gacgggcgcg
     6121 gttttgccca aacgcgcggc aattttctga atgcggtcga tttcggggat gctttccata
     6181 ttgaagcatt ttacgccggc attcagcgcg aactcgattt ccgcctcgct tttgcctacg
     6241 ccggaaaaaa tcgttttcgc cgcatcgccg cctgccgcca aaacgcgtgc caattcgccg
     6301 cccgacacaa tgtcaaaacc gctgcccaaa gaagcaaagt gtttgataat gctcaggttg
     6361 ccgtttgcct tgacggcgta gcagacaagc gggttcaaag cggcaaaggc ggtttgatag
     6421 ttttcaaatg ctccggtcag cgcggattgg ctgtacacat aaagcggcgt gccgaattct
     6481 tcggcaaggc gggggtaggc gacttgttcg caaaacaggg tcatattttc gttttcattt
     6541 ttgggttggt ggggcggatt gcggtttgcc ttgaagctgc aaaccggttt ggattgcgcc
     6601 gaaacgcgcc gtatcgcctt ctttgggcag gtagaggccg cctttgtaac cgcaggcgga
     6661 aagcaggagg gcggttgccg ccgcaaaaaa tacgccgtat ttcatcggta aacttccttc
     6721 ataagcgcga atgtggcaag attcggcatc ttaaacaaaa aacacgcaaa aagctatgat
     6781 gaccgaaagc gagtttatcc gcgcgagcga agcattattt gaacacatcg aagaccaaat
     6841 cgacgaaaac ggctgggatt tcgactgccg gtttgccgga aacgtcctga ccatcgaagc
     6901 cggagacggc acgcaaatca tcgtcaaccg ccacacgccc aaccaagaat tgtggattgc
     6961 cgcaaaaagc ggcggctacc atttcgccga acaaaacggc aaatggctgg caacgcgcga
     7021 cagccgcgat ttttacgacg ttttaaacga agccctgagc gcggcttcgg gcgaagcggt
     7081 tgagattgcc gaattgtgat tcaattttcg atataaaaag attgttaccc aatggcacaa
     7141 ttacctctat atcggactgc cgaaatcgga aattttactg tcggcacgcc taaagtttta
     7201 gaatcatttt ccaaacatat cccttatggt gttgtctttg aagatgatgg caacacgggc
     7261 tacttctatg ccgcttcgca agaggggatt ttggatgcct tgcacatcta caatgtcgaa
     7321 gatgtatccg acaaacatat ccccaatcat gtcttgattt tatgggatgg cgcctgcacc
     7381 atagccgcat tgtgtatcaa cgactacatc catgccgtct atgattttgt cgaacaggca
     7441 ggatattgcc gcaacggctt ccctgaagca ggcggcgaat gggtaaaagt cgaaaaccgc
     7501 gtcttggacg atgaattgct ggacaaaatc ctatcccgaa aacctacata acccgcacaa
     7561 aaggataccc aaatgcccct gttagacagt ttcaaagtcg atcacacccg tatgcacgcc
     7621 cctgccgtac gcgtggcgaa aaccatgacc acgcccaaag gcgacaccat tactgtattc
     7681 gacctgcgct tttgcatccc caataaagaa atcctgcccg aaaaaggcat ccacacgctg
     7741 gagcatttgt ttgccggctt tatgcgcgac cacttgaacg gcgccggcgt ggaaatcatc
     7801 gacatttccc cgatgggctg ccgcaccggt ttttatatga gccttatcgg cacgccttcc
     7861 gaacagcagg ttgccgatgc gtggttggct tcgatgcagg atgttttgaa tgtcaaagac
     7921 caaagtaaaa tccccgagtt gaacgaatac caatgcggca cttatctgat gcactcgctc
     7981 gccgaagcgc aacaaatcgc gcaaaacgtg ttggcgcgca aagtggcggt taaccgaaac
     8041 ggcgatttgg ctttggatga aagcctgctg aacgcttaat ccgccgcaaa tgctgtctga
     8101 acaagggttt cagacggcat ttgccttttc cgttataatc cggggttgta cgggggcggg
     8161 ttttaagccg gcatcgtcct tccctatttt tctgtccctt atcggtttca agcgggtttt
     8221 ttatgtccaa cagacctaca ctcctcctcg ttgacggctc ttcctatctc taccgcgcgt
     8281 atcacgcgat ggggcaaaac ctgactgcgc ctgacggtgc gccgacgggt gcgctgtatg
     8341 gcgtattgaa tatgttgcgc cgtttgcggt cggaatatcc gcacgattat tgcgcggtgg
     8401 tttttgatgc gaaaggcaaa aatttccgcc atcaaatgtt tgaagaatac aaggcgacgc
     8461 gcccgccgat gccggacgat ttgcgcccgc aggcggaagc cctgccggat ttggtgcgcc
     8521 tgacgggctg gccggtattg gtgattgggc aagtggaggc ggacgatgtg atcggcacgc
     8581 tggcgaaaca gggggcggaa catggtttgc gagtcattgt ttcgacgggc gataaggata
     8641 tggcgcagtt ggtggatgag cgcgttacgc tggtgaacac gatgagcgga gaaacgctgg
     8701 atattgaagg cgtgaaggca aaattcggcg ttcgccccga ccaaatccgc gattatctcg
     8761 cgctgatcgg cgacaaggtg gacaacgtac cgggcgtgga aaaatgcggc ccgaaaacgg
     8821 cggtgaaatg gctggaagcc tacggttcgt tgcaaggcgt gatggaacac gctccggaaa
     8881 tcaaaggcaa ggtcggcgaa aacctgcaag ccgcgctgcc ccaactgccg ctgtcgtatg
     8941 atttggtcac gattaaaacc gatgtggact tgcacaccga gctttcagac ggcatcgaaa
     9001 gcctgcgccg taccgcgccg aaatgggcgc agcttgcggt tgatttcaaa cgctggggct
     9061 tccgtacttg gctgaaagaa gcggaatcaa acatgaatac cggctcgacc gatgatttgt
     9121 tcggtagcga cagcatcggc gagcaggcgg ctttgaatgc ggaaataccg tctgaaaaac
     9181 gagctgaaaa agccaccgcc cctgaaaaac tggattatca agccgttacc accgaagctc
     9241 agtttgccgc cttgttggac aaactagcga aggcggacac aatcggcatt gatacggaaa
     9301 ccacgtcatt agatgcgatg aatgccgagc tggtcggcat cagcattgcg ttccaagcag
     9361 gcgaagcggt ttacatcccc gtaggccaca gcctgactgc cgcgcccgaa cagcttgatt
     9421 tgcaagacgt attaggccgt ctgaagccgc atttggaaaa ccccgccctg aaaaaaatcg
     9481 gtcaaaacct caaatacgac caacacgttt tcgccaacta cggcatcgcg ttaaacaaca
     9541 ttgccggcga cgcgatgctc gcttcctaca tcatcgagag ccatctcgga cacggcttgg
     9601 acgaattgtc cggacgctgg ctcggtttag aaaccattac ctacgaatcg ctgtgcggca
     9661 aaggcgcgaa gcaaatcagt tttgccgatg tcgccatcgg gcaggcgacc gaatacgccg
     9721 cccaagatgc cgatttcgcc ctgcgcctcg aagcgcgcct gcgcgcgcaa atggacgaca
     9781 aacaacttga aatgtatgaa aaaatggagc ttcccgtcgc gcaggtattg tttgaaatgg
     9841 aatgcaacgg cgtgcaaatc gaccgcgccg aactcgcccg ccaaagcgcg gaactcggcg
     9901 cggagctgat gaagctcgaa caggaagcct acgccgctgc aggtcaaccg tttaacctca
     9961 actcgcccaa acagctgcaa gaaatcctgt tcgacaaaat gggcatcccg acaaaagggc
    10021 tgaaaaaaac cgccaaaggc ggcatttcca ccaacgaagc cgtgctcgaa cagctcgcgc
    10081 ccgactaccc cctgcccaaa atcatcctgc aaaaccgcag cctggcgaaa ctcaaatcca
    10141 cctacaccga caaactgccc gaaatgattt cgccccggga taaccgcgtg cataccacct
    10201 atgcccaagc cgtcgccatt accggccgcc tcgccagcaa caaccccaac ctgcaaaaca
    10261 tccccattcg caccgccgaa ggccgccgcg tgcgccgcgc cttcactgca ccgccgggca
    10321 gcgtcatcgt ttccgccgac tattcccaaa tcgagctgcg cattatggcg cacctctccg
    10381 gtgacaaaac cctcattgcc gcatttcaaa gcggcgaaga cgtacaccgc cgcaccgccg
    10441 ccgaagtgtt cggcactgcg cccgaaaacg tctcgcccga gcaacgccgc tacgccaaaa
    10501 ccatcaactt cggcttaatc tacggtatgg ggcaatacgg tttggcaaaa tcattgggca
    10561 tcgacaacct ttccgccaaa aactttatcg accgctactt cgcccgttat cccggcgtcg
    10621 ccgaatacat gcagcgcacc aaagaacaag ccgccgccca aggctacgtc gaaaccctgt
    10681 tcggcagaag gctctatttg cccgacatcc gcaacaaaaa cgccaacgcc cgcgcaggag
    10741 ccgagcgtgc cgccatcaac gcccccatgc aaggcaccgc ttccgacctc atcaaacgcg
    10801 ccatgataaa cgtgcgaaac tggctttcag acggcatcgg cagcaaactg gtcatgcagg
    10861 tgcatgacga actggtgctg gaagtcgttg aaaccgaact ggattttgtc aaagaaaaac
    10921 tgccgcagat tatggcgaaa gtggacggcg gattattgga tgtaccgctg gtggctgagg
    10981 ttggcgtagg ggagaattgg gaagaggcac attgagaatg cacaagtatt gaactgttac
    11041 gttttgattt atcaaaattt aaggaagtaa aatacccgat tcgaatgatt ggaaatcaat
    11101 agcaagccgt aggtcggata cttgtatccg acaaaagcct gccatctcaa atagcagtcg
    11161 gattcgagaa tccgacctgc caaaccgggc gcggacgctc cggtcggcag ttagcgcaca
    11221 aatcgaacag aacatcacaa aaaagcctga ttcggatttt ccaatcgggc ttttttgcgc
    11281 ccgttttgtc atcccgtgaa atatccgcat aacaaaaata atgcaaaatc atgacaaaac
    11341 cggcgcgagg ttacacaaac ggatgaaatc aaccgatatt caaacacagc catttttagc
    11401 gcattttcag cgtatcttta tgcggaaaat ttcgtgaaca ggttttttgc acaggcttcc
    11461 gccgcgtttc gcgggatggg aaaccgtatt agaaaacgga cgcacgcaac ataaaccccg
    11521 aaagtgatga taagatgatg atttaacgta ctgctttaat tatttaagga attatcgtgt
    11581 ttcccgacaa atacaagttg agtttggaag aaaatatttt tctggcaaag aaagtattgg
    11641 ttgctcaaat tcacaacctc agccgttttg agaattgtca gacgaccttg ttgcagaccg
    11701 agcaaattat caatggcaaa aatgtagcct ccgcgtcact ggaagacatc caaaccatct
    11761 tgaacctgaa acgtgcctat caatatgtga tttcgcatat ttcaaacggc gaaccggtca
    11821 atatttcact ccttaaaaga atcaacaaca ttgttgccaa ggacgattct ttggtacccg
    11881 tggatttccg taccggttcg gtcggcgtaa cgctattgga cggttcccgt catgccccga
    11941 atccggtgaa ggaaattgaa gtggcccgcg tgttgcaaaa tatcggactg caaagcggtt
    12001 cgacgacgga agcagccgtt cgtttcatgc tctattgtat gcggcagcag gttttttggg
    12061 acggcaacaa acggacggca accctatttg ccaacggcct gatgatggcg gggggctgcg
    12121 gtatcctgga aatctccgaa atgcagatgc cgcgattcaa tgaaaaacta tccgcattct
    12181 atcgcaccgg cgacgatacc gatatttcca agtttgtgta tcaaaattgt atatcgggca
    12241 tagactattt cggcgcggac gaagatatat agtggattaa atttaaaccg gtacggcgtt
    12301 gcctcgcctt gccgtactat ttttactgtc tgcggcttcg ccgccttgtc ctgatttaaa
    12361 tttaatccac tatagatttc cccgactcgc cgaacatggg ctaaaaatca atttgacggt
    12421 tatcagacga tggagtaggc acaaggtggc agaaaaaggg gtttgacagt gcacggcggc
    12481 atcgtcaaac ccctttcggc atatccggcg gttaccagcg gtagcctaat ttgatgcccg
    12541 cgctgtgctg cgcttccaat tgcggcccct tggcggcggc agcgtggagg gacagcgtga
    12601 aacctttgat ttcggcgttt acgccccatt ccgcactgcg ggttttgccg aaatcctgcg
    12661 ccaatacggc ggtattgacg cgcgttcgga ctttgccgga agcggcatcg gtataggaca
    12721 ggctcaaata aggcgtgatg gaaatgtgtt gcgccggttt gaatgaataa tctgccttaa
    12781 tgcccgcgcg gtagcggttg aatgcaaggc ccggggtggc gatattgacg ttttcgtatc
    12841 ggtaatccgc tttttggacg aaatagcgcg ttgcgccgat gtgcggttcg atgccgaatc
    12901 cgccgaaacc tgcgcggtat cttgcctgaa tgccgtaatg cagcacgcgg cggcggattt
    12961 tgcctctgat gccgtctgaa aggctgccgc tactaaaacc cgcgcccgcg ctgatgccga
    13021 tgtcgaacct gccgatgccg tattgcccga aaacggcacc gtgggcaagc cgtgccgagt
    13081 tgccgatgcc gtcgtcgaag gtgttttcgg tccggttgtg cgaaaacagg atgccgacgc
    13141 gcctgctgcc gaggtttttc tgcataccga tttggcgcag gtcggtttgt tggcggtagg
    13201 cgcggaaatc ttgcgaacgg tagtgtttgg tgtcccggat gccgcttgtc caaacggcgt
    13261 tgcggcggtc ttcggcaaac acgcggtcca attcgtcctg tacggcgaaa acgctgttga
    13321 gcgtggcgga aaattcactc aaaccgctat tggcata
//
LOCUS       DAWTQS010000056        13222 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.056, whole genome shotgun sequence.
ACCESSION   DAWTQS010000056 DAWTQS010000000
VERSION     DAWTQS010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13222
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.056"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(40..975)
                     /gene="hemC"
                     /locus_tag="WNA25_001624"
     CDS             complement(40..975)
                     /gene="hemC"
                     /locus_tag="WNA25_001624"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFA9824343.1"
                     /translation="MNPKKLVIASRESLLAMWQAKHIQGRLKALYPDCEVEILGMTTR
                     GDRILDRTLSKVGGKGLFVKELEQSLQDGRADLAVHSIKDVPMDLPEGFALAAISERA
                     NPFDAFVSNRYARLEEMPEGAVVGTSSLRREAQLRARYPHLLIKPLRGNVQTRLSKLD
                     NGEYDAIILAAAGLQRLELDERIRMILSESDSLPAAGQGALGIEIATHREDLYEVLKP
                     LNHDTTHACVTAERALARALGGSCQVPLAAYCTEENGLLILRGLVGHPDGSIVLQADA
                     QAPAGYADALGRAVAKKLADDGAQELIGAVLNTEN"
     gene            1094..1609
                     /gene="ybeY"
                     /locus_tag="WNA25_001625"
     CDS             1094..1609
                     /gene="ybeY"
                     /locus_tag="WNA25_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFA9824344.1"
                     /translation="MKRAKKYPFLTLQRQRFHLNFENVSSAAGIPAERDFYRWAWSAL
                     KNEYLRADIGLILLDEEEARAYNRDYRGKDYATNVLSFALNEGEILPCQVSERLYGDL
                     IICPQVVLKEAAEQGKTPERHFAHLTIHGTLHLMGYDHIKDDEAEIMEAEEIRLMRAA
                     GYPNPYREDGH"
     gene            1611..2435
                     /locus_tag="WNA25_001626"
     CDS             1611..2435
                     /locus_tag="WNA25_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFA9824345.1"
                     /translation="MDGAQPKTNFFERLIARLAREPDSAEDVLNLLRQAHEQEVFDAD
                     TLTRLEKVLDFAELEVRDAMITRSRMNVLKENDSIERITAYVIDTAHSRFPVIGEDKD
                     EVLGILHAKDLLKYMFNPEQFHLKSVLRPAVFVPEGKSLTALLKEFREQRNHMAIVID
                     EYGGTSGLVTFEDIIEQIVGDIEDEFDEDESADNIHSVSAERWRIHAATEIEDINAFF
                     GTEYGSEEADTIGGLVIQELGHLPVRGEKVLIGGLQFTVARADNRRLHTLMATRVK"
     gene            complement(2578..3957)
                     /gene="nhaC"
                     /locus_tag="WNA25_001627"
     CDS             complement(2578..3957)
                     /gene="nhaC"
                     /locus_tag="WNA25_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFA9824346.1"
                     /translation="MFAFKSLLDMPRGEALAVVVALIAAMGYTIISLEWLPHMSIIAA
                     IVVLILYGLARGLKYNDMQAGMIGALNQGMGAVYLFFFIGLMVSALMMSGAIPTLMYY
                     GFGLISPTYFYFSAFALCSVIGVSIGSSLTACATAGVAFMGMAAAFQADMAMTAGAIV
                     SGVFFGDKMSPLSDTTGISASIVGIDLFEHIKNMMYTTIPAWLISAALMLWLLPSVAA
                     QDLNSVESFRSQLEATGLVHGYSLIPFALLVVLALMRVNAVVAMLFTVIAAVAVTYLH
                     STPDLRQLGAWFYGGYKLEGEAFKDIAKLISRGGLESMFFTQTIVILGMSLGGLLFAL
                     GVIPSLLEAVRTFLTNAGRATFSVAMTSVGVNFLIGEQYLSILLSGETFKPVYDKLGL
                     HSRNLSRTLEDAGTVINPLVPWSVCGVFISHALGVPVWEYLPYAFFCYLSLALTLLFG
                     WTGLTLSKK"
     gene            complement(4247..5470)
                     /locus_tag="WNA25_001628"
     CDS             complement(4247..5470)
                     /locus_tag="WNA25_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HFA9824347.1"
                     /translation="MSAQSQNNHTSPLVVLTTLFFMMGFITCMNDILIPHLKEIFDLS
                     YVQAMLIQFCFFTAYAVMSIPMGAFVGKVGYKNGVIGGFLLTAVGCLLFYPAAGSHSY
                     AVFLGALFILASGVTLLQVAGNPYVTLLAKPGKESATLTLVQAFNALGTTIAPQIGAF
                     LILADATQTVSKAEQISSVQIPYLGLAGLLIILAVFVKMIRLPDARKIAAEESAHNHD
                     GKTGVWQYKHLVFGTAGIFCYVGAEVSIGSLMVNVLGYLKGLDHASAAHYLSFYWGGA
                     MVGRFLGSAVMAKFAPNRYLAFNASAAVVLLAVAMATGSGNADVAMWSLLAIGFFNSI
                     MFPTIFSLATKGLGKFTNAASGVLCTAIVGGAVVPVIQGWAADTYTLMSSFVVSVICY
                     LYIVFFAVYGYRADK"
     gene            5907..6320
                     /locus_tag="WNA25_001629"
     CDS             5907..6320
                     /locus_tag="WNA25_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFA9824348.1"
                     /translation="MIPSYTYAALAFSAFTSATLLPGTSEAAFALFVRNFPKHAYGAL
                     LCAGLANGLGSMVSYWMGRLLPSRKMPSEKTLNLIRRFGIWLLAFAWLPVVGDALPLT
                     AGWLRLNPWTSGLMLVIGKTARYAFILWGMQYYAA"
     gene            6367..6996
                     /gene="nth"
                     /locus_tag="WNA25_001630"
     CDS             6367..6996
                     /gene="nth"
                     /locus_tag="WNA25_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFA9824349.1"
                     /translation="MNRQIRQEIFERFRAANPHPTTELNFNSPFELLIAVLLSAQATD
                     VGVNKATAKLFPVADTPQAMLDLGLDGVMEYTKTIGLYKTKSKHIMQTCRILLEKYNG
                     EVPEDREALESLPGVGRKTANVVLNTAFGHPVMAVDTHIFRVSNRTKIAPGKDVREVE
                     DKLMRFIPKEFLMDAHHWLILHGRYTCKALKPQCQTCIINDLCEYPAKS"
     gene            7134..8633
                     /locus_tag="WNA25_001631"
     CDS             7134..8633
                     /locus_tag="WNA25_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HFA9824350.1"
                     /translation="MFKKYQYFALAALCAALLAGCEKAGSFFGADKKEASFVERIEHT
                     KDDGSVSMLLPDFAQLVQSEGPAVVNIQAAPAPRTQNGSGNAETDSDPLADSDPFYEF
                     FKRLVPNMPEIPQEEADDGGLNFGSGFIISKNGYILTNTHVVAGMGSIKVLLNDKREY
                     TAKLIGSDVQSDVALLKIDATEELPVVKIGNPKNLKPGEWVAAIGAPFGFDNSVTAGI
                     VSAKGRSLPNESYTPFIQTDVAINPGNSGGPLFNLKGQVVGINSQIYSRSGGFMGISF
                     AIPIDVAMNVAEQLKNTGKVQRGQLGVIIQEVSYGLAQSFGLDKASGALIAKILPGSP
                     AERAGLQAGDIVLSLDGGEIRSSGDLPVMVGAITPGKEVSLGVWRKGEEITIKAKLGN
                     AAEHTGASSKTDEAPYTEQQSGTFSVESAGITLQTHTDSSGKHLVVVRVSDAAERAGL
                     RRGDEILAVGQVPVNDEAGFRKAMDKAGKNVPLLVMRRGNTLFIALNLQ"
     gene            8836..10356
                     /locus_tag="WNA25_001632"
     CDS             8836..10356
                     /locus_tag="WNA25_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HFA9824351.1"
                     /translation="MQLIDYSHSFFSVVPPFLALALAVITRRVLLSLGIGILVGVAFL
                     VGGNPVDGLTHLKDMVVGLAWADGDWSLGKPKILVFLILLGIFTSLLTYSGSNQAFAD
                     WAKRHIKNRRGAKMLTACLVFVTFIDDYFHSLAVGAIARPVTDKFKVSRAKLAYILDS
                     TASPMCVLMPVSSWGASIIATLAGLLVTYKITEYTPMGTFVAMSLMNYYALFALIMVF
                     VVAWFSFDIGSMARFEQAALNEAHDETAASDATKGRVYALIIPVLALIASTVSAMIYT
                     GAQASETFSILGAFENTDVNTSLVFGGTCGVLAVVLCTFGTIKTADYPKAVWQGAKSM
                     FGAIAILILAWLISTVVGEMHTGDYLSTLVAGNIHPGFLPVILFLLASVMAFATGTSW
                     GTFGIMLPIAAAMAVKVEPALIIPCMSAVMAGAVCGDHCSPISDTTILSSTGARCNHI
                     DHVTSQLPYALTVAAAAASGYLALGLTKSALLGFSTTGIVLAVLIFLLKDKKRADV"
     gene            10413..11498
                     /gene="nagZ"
                     /locus_tag="WNA25_001633"
     CDS             10413..11498
                     /gene="nagZ"
                     /locus_tag="WNA25_001633"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFA9824352.1"
                     /translation="MTVPHIPRGPVMADIAAFRLTEEEKQRLLDPAIGGIILFRRNFQ
                     NIEQLKTLTAEIKALRTPELIIAVDHEGGRVQRFIEGFTRLPAMSTLGEIWDSEGAET
                     AETHAEHIGWVLATELSACGIDLSFTPVLDLDWGNCAVIGNRSFHRNPEAVARLALAL
                     QKGLAKGGMKSCGKHFPGHGFVEGDSHLVLPEDGRSLDELEAADLAPFRIMSREGMAA
                     VMPAHVVYPQVDTKPAGFSEIWLKQILRRDIGFKGVIFSDDLTMEGACGAGGIKERAR
                     ISFEAGCDIILVCNRPDLVDELRDGFTIPDNQDLAGRWQYMENSLGHEAVQAVIQTTG
                     FQAAQAFVAGLASPQDTAGGVKVGEAF"
     gene            11723..11799
                     /locus_tag="WNA25_001634"
     tRNA            11723..11799
                     /locus_tag="WNA25_001634"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11757..11759,aa:Pro,seq:cgg)
     gene            complement(12135..12773)
                     /locus_tag="WNA25_001635"
     CDS             complement(12135..12773)
                     /locus_tag="WNA25_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFA9824353.1"
                     /translation="MKKISVAPENPQYRIVEIFESLQGEGRNTGMPAVFVRLGKCNLA
                     CGWCDTDYLTFGMMSLSDILGCLKTYAARNIIITGGEPTIQPHLDMLLDTLKAEGYFL
                     CLETNGLKPAPPQIDYVATSPKACYAAKYETNCIAEADEVRIVADGDVLAFCENMERK
                     IRARHYYLSPCEQNGVMNIYDTIRQIGILNSRPDAPVHWQLSVQTHKWAGIE"
     gene            complement(12770..13138)
                     /locus_tag="WNA25_001636"
     CDS             complement(12770..13138)
                     /locus_tag="WNA25_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFA9824354.1"
                     /translation="MKLLSTILVLLVAAEHFYIAWLEMTQIPGEKAAEMFKLPYEFME
                     QKRVQTLFGNQGLYNGFLAVGLLWAQFAAPDNAVYGATVLFLGFVLIAAAWGAFSSGN
                     KGILVKQGLPAFLAAAVLAV"
BASE COUNT         3290 a         3782 c         3340 g         2810 t
ORIGIN      
        1 acaataactt atattatttt ctttaaattt tcgataaaat caattttccg tattcaatac
       61 tgctccaatc aattcctgcg caccgtcgtc cgccagtttt ttggcaaccg cacgtccaag
      121 cgcatcggca tatccggcag gggcttgcgc gtccgcctgc aacacaatcg atccgtcggg
      181 gtgcccgacc aatccgcgca agatcagcag cccgttttct tcagtgcaat acgcggccag
      241 cggcacttgg cagcttccgc ccaaagcgcg cgccaaagcg cgttcggcgg taacgcaggc
      301 gtgtgtggta tcgtggttta acggcttcaa gacttcgtac aaatcttcgc gatgcgtggc
      361 aatttcaata cccaatgcgc cttgtccggc ggcaggcagg ctgtcggatt ccgacaaaat
      421 catgcggatg cgttcatcca attccagacg ctgcaacccg gcggcagcca agataattgc
      481 atcgtattcg ccgttgtcga gtttggacaa acgggtttgc acattgccgc gcaaaggttt
      541 gataagcaaa tgcggatagc gcgcgcgcaa ctgggcttca cggcgcaggc tggatgtgcc
      601 gacaaccgcg ccttcgggca tttcttccaa acgcgcgtat cggttggaca caaacgcgtc
      661 aaacggattg gcgcgttcgc tgatggcggc aagggcgaag ccttcgggca aatccatcgg
      721 cacgtcctta atcgaatgca ccgccaaatc ggcgcgcccg tcttgaaggg attgttccaa
      781 ctctttgaca aacaagcctt tcccgccgac ttttgacaaa gttctgtcca aaatccggtc
      841 gccgcgcgtg gtcatgccca aaatttcgac ttcgcaatcg ggatacagcg ccttcagacg
      901 gccttggata tgtttcgcct gccacatggc aagcaggctt tcgcggctgg cgataacaag
      961 ttttttcggg ttcatgttca atgcgttcaa agaatggaaa cggcgtggag tctatcatat
     1021 tttgaacgtt tcttcagacg gcaccgtcag gtctatgtta tatttacgcc cacaaaacac
     1081 gaaacccgcc acaatgaaac gcgccaaaaa ataccctttc ttaacgttgc agcggcaacg
     1141 tttccatttg aactttgaaa acgtctcttc cgccgccggc atccccgccg aacgcgattt
     1201 ttaccgctgg gcgtggtctg ccttgaaaaa cgaatacctc cgcgccgaca tcggtttgat
     1261 tcttttggac gaagaagaag cccgcgccta caaccgcgac taccggggca aagattatgc
     1321 caccaatgta ttgagtttcg cgctcaacga aggcgaaatc ctgccctgcc aagtttcgga
     1381 aagactgtac ggcgatttga ttatctgccc gcaagtcgtt ttaaaagaag ccgccgaaca
     1441 gggcaaaacg cctgaacggc attttgccca cttaaccata cacggcacgc tgcacctgat
     1501 gggatacgac cacatcaaag acgatgaggc cgaaataatg gaagccgaag aaatccgcct
     1561 gatgcgggcg gcaggctatc ccaaccccta ccgagaggac ggacattaaa atggacggcg
     1621 cacaaccgaa aacaaatttt tttgaacgcc tgattgcccg actcgcccgc gaacccgatt
     1681 ccgccgaaga cgtattaaac ctgcttcggc aggcgcacga acaggaagtt tttgatgccg
     1741 acacactgac ccggctggaa aaagtattgg actttgccga gctggaagtg cgcgatgcga
     1801 tgattacgcg cagccgcatg aacgtattga aagaaaacga cagcatcgaa cgcatcaccg
     1861 cctacgtcat cgataccgcc cattcgcgct tccccgtcat cggcgaagac aaagacgaag
     1921 ttttgggcat tttgcacgcc aaagacctgc tcaaatatat gttcaacccc gagcagttcc
     1981 acctgaaatc cgtcttgcgc cctgccgttt tcgtgcccga aggcaaatct ttgaccgccc
     2041 ttttaaaaga gttccgcgaa cagcgcaacc atatggcaat cgtcatcgac gaatacggcg
     2101 gcacgtcggg tttggtcacc tttgaagaca tcatcgagca aatcgtcggt gacatcgaag
     2161 acgagtttga cgaagacgaa agcgccgaca acatccactc cgtttccgcc gaacgctggc
     2221 gcatccacgc ggctaccgaa atcgaagaca tcaacgcctt tttcggtacg gaatacggca
     2281 gcgaagaagc cgacaccatc ggcggcttgg tcattcagga attgggacac ctgcccgtgc
     2341 gcggcgaaaa agtccttatc ggcggtttgc agttcaccgt cgcccgcgcc gacaaccgcc
     2401 gcctgcacac gctgatggcg acccgcgtga agtaagcaga gcctgcccgc accgccgttt
     2461 ctgcacagtt taggatgacg gtacggtcgt tttctgtttc aatccgcccc atccgccaaa
     2521 cataaaaatg ccgtctgaaa ccggaaaaca ggttcagacg gcatttcgct tatccgctta
     2581 ttttttgctc aaagtcagcc ccgtccagcc gaataacagg gttaaagcca aactcaaata
     2641 gcagaaaaag gcataaggca gatattccca aacgggtacg ccaagggcgt ggctgataaa
     2701 tacgccgcac acgctccacg gcacgagcgg gttaatcacc gtccccgcat cttccagagt
     2761 ccgcgacagg ttgcgcgaat gcaggccgag tttgtcgtaa acgggtttga acgtttctcc
     2821 cgaaagcagg atgctcaaat attgctctcc aatcaggaaa ttgaccccga ccgaagtcat
     2881 ggcaacgctg aacgtcgcgc gtccggcatt cgtcaagaag gtacggacgg cctccagcag
     2941 ggaaggaatc acaccgagcg caaacagcag cccgcccaaa ctcataccga ggataacgat
     3001 ggtctgcgta aagaacatac tctccaagcc gccgcgcgaa atcagtttgg caatgtcttt
     3061 aaacgcttcg ccttcgagtt tgtagccgcc ataaaaccac gcgccgagct gacgcagatc
     3121 gggcgtgctg tgcagatacg ttacggcaac ggcggcaatg acggtaaaga gcatggcgac
     3181 cacggcattg acgcgcatca atgccaaaac gaccaacagt gcaaacggaa tcagcgaata
     3241 gccgtgcacc aatcccgtgg cttcaagctg gctgcggaag gattcgacgc tgttcaaatc
     3301 ctgcgcggcg acgctgggaa gaagccaaag catcagtgcc gcgctgataa gccacgcggg
     3361 gatggtggtg tacatcatgt ttttgatgtg ttcaaacagg tcgataccga cgatggacgc
     3421 ggaaatgccc gtggtgtcgg aaagcgggga cattttatcg ccgaaaaaca caccggaaac
     3481 aatcgcgccc gccgtcatcg ccatatcggc ctgaaacgcc gccgccatcc ccataaaggc
     3541 aacgccggca gtggcgcagg cggtcaggct gctgccgatg gacacgccga tgacggaaca
     3601 cagcgcgaag gcggaaaaat aaaaataagt cggggaaatc agcccgaaac cgtaatacat
     3661 cagcgtcgga atcgcgccgc tcatcatcag cgcgctgacc atcagcccga tgaagaaaaa
     3721 caggtaaacc gcgcccatac cctgattcaa cgcgcctatc atccctgcct gcatatcgtt
     3781 gtatttcaaa ccgcgcgcca agccgtacaa aatcagcacg acgatggcgg caataatgga
     3841 catatgcggc agccactcca atgaaatgat ggtatagccc attgcggcaa tcagagcgac
     3901 gacgacggca agtgcctcac cgcgcggcat atcgagtaag gatttgaaag caaacatgat
     3961 tctttgcccg atttgatata aagttaacgg attctaacac accatgccga atctgcccga
     4021 cgttttcaga cggcatttta gcgttttaca aaagccgggc agaaacaaaa cggcaatttt
     4081 ctcgatttaa attaattttt ttgaaatata atttttattt ttttatttgt tggcatataa
     4141 gacttttatg gattgaagta ttaaaacctc ggcacacacg ccgcaaacac cggtacgcaa
     4201 aaatgccgtc tgaaagatgt tcagacggca tttctcaaga aaaagattat ttgtccgccc
     4261 tatatccgta caccgcaaaa aacacgatat acaggtaaca gataacggaa acgacaaacg
     4321 aagacatcag ggtgtaagta tccgccgccc aaccttgaat taccggcacg accgcaccgc
     4381 cgacaatcgc ggtacacagc acaccggaag ccgcgttggt gaatttgccc aaccctttgg
     4441 ttgccaaaga gaaaatcgtc gggaacataa tcgagttgaa aaaaccgatg gcaagcagcg
     4501 accacatcgc cacatccgca ttgccgctgc ccgtcgccat cgcgacggca agcagcacga
     4561 ccgcagccga tgcgttaaac gccaaataac ggttgggcgc gaatttcgcc attaccgccg
     4621 aaccgaggaa acgtccgacc atcgctccgc cccaataaaa cgacaggtaa tgcgcggcag
     4681 aagcatgatc cagccctttc agataaccca acacattgac catcagcgaa ccgatagaca
     4741 cctccgcacc gacgtagcag aaaatgcctg ccgtaccgaa cacgagatgt ttgtattgcc
     4801 atacgccggt tttgccgtcg tggttgtgcg cgctttcctc ggcggcaatt ttgcgcgcgt
     4861 cgggcagccg gatcattttc acgaaaacgg caaggataat cagcagcccc gccagtccca
     4921 aatagggaat ctgtacggaa gaaatctgtt ccgccttgct gacggtttgg gttgcgtccg
     4981 ccaaaatcag gaacgcgccg atttgaggcg caatggtcgt acccaaagcg ttaaacgcct
     5041 gaaccagcgt cagcgttgcc gattccttgc cgggtttcgc cagcagggta acataaggat
     5101 taccggcgac ctgaagcagc gttacgccgg aagccaaaat aaacaacgcg cccaaaaata
     5161 ccgcgtaaga atggctgccc gcagcaggat aaaacagcag gcacccgacc gccgtcagca
     5221 gaaagccgcc gataacgccg tttttgtagc cgactttgcc gacaaaagcc cccatcggga
     5281 tggacattac cgcataggcg gtaaaaaagc aaaattgaat cagcatcgcc tgaacgtaag
     5341 agaggtcgaa aatttctttc aaatgaggga taaggatgtc gttcatgcag gtaataaaac
     5401 ccatcatgaa gaacagcgtg gtcaagacga ccaatgggga cgtatggttg ttttgtgatt
     5461 gcgcagacat gatttgcctt tcgtggtgtc ggaatattgc ccgtttcaag aaaatagaaa
     5521 ttgacatttc ttaacggcaa tgcaaatcat actgatattt gaacattttg caaactttat
     5581 cggatgcagg caatgcgccg atctgaaatt caagcagcag aaattaatat tttacagttg
     5641 tttgcatagg ttttaataga ttttaaaaaa ttccggcggg cttgcaaaaa ccgttcccaa
     5701 taaaatgaaa atcctccgcc gtatccggaa aattcggaaa tgccgcatcg gggcatcaaa
     5761 accggagcgg aaagaatgtc gtatgcccga caaataaagg ggcatacggc tgtgccgtat
     5821 ttttgcaccg tcctccgccc ttcggcgggg gcgtgaaaat accgtataat ccgtcgccga
     5881 tttttacctg tttgggactg tttgcaatga taccctccta cacctacgcc gccctcgctt
     5941 tttccgcctt cacttccgcc accctcctgc ccggcacgtc cgaagccgcc ttcgccctgt
     6001 tcgtccgcaa cttccccaaa cacgcatacg gcgcgttgct gtgcgccggc cttgccaacg
     6061 gattgggcag tatggtttcc tattggatgg gacgtttgct gccttcccga aaaatgccgt
     6121 ctgaaaaaac actgaatctg atacggcgtt tcggtatttg gctgcttgcg tttgcgtggc
     6181 tgcccgtcgt cggcgacgca ctgccgctta ccgccggctg gctgcggttg aatccgtgga
     6241 caagcgggct gatgctggtt atcggcaaaa cggcgcgtta cgccttcatt ctgtggggaa
     6301 tgcaatatta cgccgcctga accgtgcgtt ataatgccga ccgcacaaac cgactgcaca
     6361 aaaaaaatga acagacaaat ccgccaagaa atcttcgagc gtttccgcgc cgccaacccc
     6421 catccgacca ccgagctgaa tttcaactcc cctttcgagc ttttaatcgc cgttctgctt
     6481 tcggcgcagg cgaccgatgt cggcgtaaac aaggcgacgg cgaagctgtt tccggttgcc
     6541 gatacgccgc aggcgatgct ggatttgggc ttggacggcg tgatggaata cacgaaaacc
     6601 atcgggctgt ataaaaccaa gtccaaacac attatgcaaa cctgccgcat cctgctggaa
     6661 aaatacaacg gcgaagtgcc ggaagaccgc gaggctttgg aatcgttgcc tggcgtgggg
     6721 cgcaaaacgg caaacgtggt attgaacaca gcgttcggac accccgttat ggcggtcgat
     6781 acgcatattt tccgcgtgtc caaccgaacc aaaatcgcac ccggaaaaga tgtacgcgaa
     6841 gtcgaagaca aactgatgcg cttcattcct aaagaatttc tgatggacgc gcaccactgg
     6901 ctgattttgc acggacgcta cacctgcaag gctttaaagc cgcaatgcca aacctgcatc
     6961 atcaacgatt tgtgcgaata tcccgccaaa tcctgaccgc cgccgccctt caaaatgaaa
     7021 cgcttgaaaa ttcggacgga cgttgtagaa tttcaaccta tgccgtctga agccgacaaa
     7081 atgcgcttca gacggcatta aagccggtac cggcatccga caaaggacaa aacgtgttca
     7141 aaaaatacca atacttcgct ttggcggcac tgtgtgccgc cttgctggca ggctgcgaaa
     7201 aggcaggcag ctttttcggt gcggacaaaa aagaagcatc cttcgtagaa cgcatcgaac
     7261 acaccaaaga cgacggcagt gtcagtatgc tgctgcccga ctttgcccaa ctggttcaaa
     7321 gcgaaggccc ggcagtcgtc aatattcagg cagcccccgc cccgcgcacc caaaacggca
     7381 gcggcaatgc cgaaaccgat tccgacccgc ttgccgacag cgacccgttc tacgaatttt
     7441 tcaaacgcct cgtcccgaac atgcccgaaa tcccccaaga agaagcagat gacggcggat
     7501 tgaacttcgg ttcgggcttc atcatcagca aaaacggcta catcctgacc aatacccacg
     7561 tcgttgccgg tatgggcagt atcaaagtcc tgctcaacga caagcgcgaa tataccgcca
     7621 aactcatcgg ttcggatgtc caatccgatg tcgcccttct gaaaatcgac gcaacggaag
     7681 agctacccgt cgtcaaaatc ggcaatccca aaaatttgaa accgggcgaa tgggtcgctg
     7741 ccatcggcgc gcccttcggc tttgacaaca gcgtgaccgc cggcatcgtg tccgccaaag
     7801 gcagaagcct gcccaacgaa agctacacac ccttcatcca aaccgacgtt gccatcaatc
     7861 cgggcaattc cggcggcccg ctgttcaact taaaaggaca ggtcgtcggc atcaattcgc
     7921 aaatatacag ccgcagcggc ggattcatgg gcatctcctt tgccatcccg attgacgttg
     7981 ccatgaatgt cgccgaacag ctgaaaaaca ccggcaaagt ccaacgcgga caactgggcg
     8041 tgattattca ggaagtatcc tacggtttgg cacagtcgtt cggtctggat aaagccagcg
     8101 gcgcattgat tgccaaaatc cttcccggca gccccgcaga acgtgccggc ctgcaggcgg
     8161 gcgacatcgt cctcagcctc gacggcggag aaatacgttc ttccggcgac cttcccgtca
     8221 tggtcggcgc cattacgccg ggaaaagaag tcagcctcgg cgtatggcgc aaaggcgaag
     8281 aaatcacaat caaagccaag ctgggcaacg ccgccgagca taccggcgca tcatccaaaa
     8341 cagatgaagc cccctacacc gaacagcaat ccggtacgtt ctcggtcgaa tccgcaggca
     8401 ttacccttca gacacatacc gacagcagcg gcaaacacct cgtcgtcgta cgggtttccg
     8461 acgcggcaga acgcgcaggc ttaaggcgcg gcgacgaaat cctcgccgtc gggcaagtcc
     8521 ccgtcaatga cgaagccggt ttccgcaaag ctatggacaa ggcaggcaaa aacgtccccc
     8581 tgctggtcat gcgccgtggc aacacgctgt tcatcgcatt aaacctgcaa taacgcacat
     8641 aatttaacca aaatattttt aaatgataaa atgcccgtca ataccgatta acgggcattt
     8701 tacaaaaatc agttggttga ttttaaacaa atatttttta cgacaaaaaa tatttccacc
     8761 cattttgcca acaaaaccca aaccggaggc ggacagatac ccacccatcc ggacgcttaa
     8821 caccccccat aaaccatgca gctgattgac tattcacatt catttttctc ggttgtgcca
     8881 ccctttttgg cactggcact tgccgtcatt acccgccgcg tactgctgtc tttaggcatc
     8941 ggtattttgg tcggcgttgc ctttttggtc ggcggcaacc ccgtcgacgg tctgacacac
     9001 ctgaaagaca tggtcgtcgg cttggcttgg gcagacggcg attggtcgct gggcaaacca
     9061 aaaatcttgg ttttcctgat acttttgggc attttcactt cactgctgac ctactccggc
     9121 agcaatcagg cgtttgccga ctgggcaaaa cggcacatta aaaaccggcg cggcgcgaaa
     9181 atgctgaccg cctgcctcgt gttcgtaacc tttatcgacg actatttcca cagcctcgcc
     9241 gtcggtgcga ttgcccgccc cgttaccgac aagtttaaag tttcccgcgc caaactcgcc
     9301 tacatcctcg actccactgc ctcgcccatg tgcgtgctga tgcccgtttc aagctggggc
     9361 gcgtcgatta tcgccacgct tgccggattg ctcgttacct acaaaattac cgaatacacg
     9421 ccgatgggga cgtttgttgc catgagcctg atgaactatt acgcgctgtt tgccctgatt
     9481 atggtattcg tcgtcgcatg gttctccttc gacatcggct cgatggcgcg tttcgaacag
     9541 gctgcgttga acgaagccca cgacgaaacc gccgcttcag acgctaccaa aggtcgtgtt
     9601 tacgcattga ttattcccgt tttggcctta atcgcctcaa cggtttccgc catgatctac
     9661 accggcgcgc aggcaagcga aaccttcagc attttggggg catttgaaaa taccgacgta
     9721 aacacttcgc tggtattcgg cggcacttgc ggcgtgcttg ccgtcgtcct ctgcacgttc
     9781 ggcacgatta aaaccgccga ttatcccaaa gccgtgtggc agggtgcgaa atccatgttc
     9841 ggcgcaatcg ccattttaat cctcgcctgg ctcatcagta cggttgtcgg cgaaatgcac
     9901 acgggcgact acctctccac gctggttgcg ggcaacatcc atcccggctt cctgcccgtc
     9961 atcctcttcc tgctcgccag cgtgatggcg tttgccacag gcacaagctg ggggacgttc
    10021 ggcattatgc tgccgattgc cgccgccatg gcggtcaaag tcgaacccgc gctgattatc
    10081 ccgtgtatgt ccgcagtaat ggcgggggcg gtatgcggcg accactgttc gcccatctcc
    10141 gacacgacca tcctgtcgtc caccggcgcg cgctgcaacc acatcgacca cgttacctcg
    10201 caactgcctt atgccctgac ggttgccgcc gccgccgcat cgggctacct cgcattgggt
    10261 ctgacaaaat ccgcgctgtt gggctttagc acgaccggta ttgtattggc ggtgctgatt
    10321 tttctgttga aagataaaaa acgcgccgac gtttgacctt cccccctgtt cagacggcat
    10381 atgccgtctg aagttttttg gagcaaaccg caatgaccgt cccccatatt ccgcgcggcc
    10441 ccgtcatggc agacattgcc gccttccgcc tgaccgaaga agaaaaacaa cgcctgctcg
    10501 atcccgcaat cggcggcatc atcctcttcc gccgcaactt ccaaaacatc gaacaactca
    10561 aaacactcac cgccgaaatc aaagccctgc gcacacccga actcattatc gccgtcgatc
    10621 acgaaggcgg cagggtgcaa cgtttcatcg aaggttttac ccgcctgccc gcaatgagta
    10681 cgctcggcga gatttgggac agtgaaggcg cggaaactgc cgaaactcat gccgaacaca
    10741 tcggatgggt tttggcaacc gagctttccg cctgcggcat cgatttgtcc ttcacgcccg
    10801 tcttagattt ggactgggga aactgcgccg tcatcggcaa ccgcagcttc caccgcaatc
    10861 ccgaagcggt cgcccgcctc gccctcgccc ttcaaaaagg tttggcaaaa ggcggtatga
    10921 aatcatgcgg caaacatttc cccggacacg gatttgtcga aggggacagc catttggtgc
    10981 tacccgaaga cgggcgcagc ctcgacgaac tcgaagccgc cgacctcgcc cccttccgca
    11041 ttatgagccg cgaaggtatg gcggcggtca tgcccgccca cgtcgtttat ccgcaagtgg
    11101 acacaaagcc cgcagggttt tccgaaatct ggctcaaaca aattttgcgc cgcgatatcg
    11161 gattcaaagg cgtgattttt tctgacgatt tgaccatgga gggcgcgtgc ggggcaggcg
    11221 gcatcaaaga acgcgcccgc atttcctttg aggcaggttg cgacatcata ttggtctgca
    11281 accgtcccga cttggtcgat gaattgcgcg acggttttac cattcccgac aaccaggatt
    11341 tggccggacg ttggcaatac atggaaaact cgctcggtca tgaggcggta caagccgtta
    11401 tacagacaac gggtttccaa gcggcgcaag cctttgttgc cggccttgcc tcgccgcagg
    11461 acacggcggg cggcgtaaaa gtcggagagg ccttttaaac cggctgcgtt tcaacaatct
    11521 gccgatttca accgttcgtc agggaaaggc aatgattttt tcggtcaaat agaaagttat
    11581 cttattgaat tactttgaga taaggatgct tatcaatact gtattcaaac acagcccttt
    11641 aaatccgccc gacacgcggc aaaaaggcaa aacggtatag gcacgccgta ttttttgcgc
    11701 tataatcaca ccttaattaa ttcggaatgt agcgcagccc ggtagcgcac ttcgttcggg
    11761 acgaaggggt cggaggttcg aatcctctca ttccgaccat attcagaccg cctcgacatc
    11821 gattgccggg gcggtttttc ccttttggcg gcaacaaaaa aatgccgtct gaaacagaat
    11881 gctgtttcag acggcatttc gttttcccca atcctgccat ttgcgcgcac tgacaaaggg
    11941 tatcggcaag gttcaaaaga ccatcggaat ctaggaagcc tggcgtgtac ccaaccgcgg
    12001 catcttattc attaaggctt cgtcctgatt gggcggcgga aatgtcggca gtttcacgcg
    12061 gaagcggcac actttccccg tatgtcaaac atcggcaaga aggtacgcct gccctttggg
    12121 ttacactgct taaactactc tatccccgcc catttgtgcg tctgcacgct caactgccaa
    12181 tgcacgggcg cgtcggggcg gctgtttaaa ataccgattt ggcggatggt gtcgtagatg
    12241 ttcatcacgc cgttttgttc gcagggagaa agatagtaat ggcgcgcacg gattttgcgt
    12301 tccatatttt cgcaaaacgc aaggacatcg ccgtcggcaa caatccgtac ttcgtcggct
    12361 tcggcgatgc agttggtttc atacttggcg gcgtagcagg ctttggggct ggtggcgaca
    12421 tagtcgattt gcggcggcgc gggcttgagc ccgttggttt cgagacagag gaaatagcct
    12481 tccgccttga gcgtgtccag cagcatatcg agatgcggct ggatggtcgg ctctccgccg
    12541 gtgatgatga tgttgcgggc ggcataagtt ttcagacagc ctaagatgtc ggacaggctc
    12601 atcataccaa atgtcaaata atcggtatca caccagccgc acgccagatt gcatttgccc
    12661 aagcggacga aaacggcagg catacccgtg tttcggccct caccttgcag gctttcaaag
    12721 atttcgacga tgcggtactg gggattttcg ggggcgacgc tgattttttt cataccgcca
    12781 acaccgccgc cgccaaaaat gcgggcaaac cttgtttgac gagtatgcct ttgttgccgg
    12841 acgaaaacgc accccacgcg gcggcaatca ggacgaagcc gagaaacagt accgtcgcgc
    12901 cgtacacggc attatccgga gcggcaaact gcgcccacaa aagcccgacg gcaagaaagc
    12961 cgttgtacag cccttggttg ccaaacaggg tctgcacgcg tttctgttcc ataaattcgt
    13021 aaggcagctt gaacatttcc gccgcttttt cgccgggaat ctgcgtcatt tcaagccagg
    13081 cgatgtagaa atgttcggcg gcgacgagga ggacgagaat ggtggagagg agtttcatgg
    13141 gtgttccttt caatgggtgt gccggtatga aatgccgtct gaaaaaacgg ggaatccgtg
    13201 caggagaaaa aaccgcttta cg
//
LOCUS       DAWTQS010000057        13220 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.057, whole genome shotgun sequence.
ACCESSION   DAWTQS010000057 DAWTQS010000000
VERSION     DAWTQS010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13220
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.057"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(201..686)
                     /gene="purE"
                     /locus_tag="WNA25_001637"
     CDS             complement(201..686)
                     /gene="purE"
                     /locus_tag="WNA25_001637"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFA9824355.1"
                     /translation="MIQIGIIMGSNSDWPVMRQAAQFLEEFGVEYEARVVSAHRTPDL
                     MFEYAETARARGIKAIIAGAGGAAHLPGMVAAKTTVPVLGVPVPSKYLRGEDSLLSIV
                     QMPKGVPVATFAIGEAGAANAALFAISMLANENPELAQKLADFRAKQEQTVLNMELEQ
                     I"
     gene            complement(734..1447)
                     /locus_tag="WNA25_001638"
     CDS             complement(734..1447)
                     /locus_tag="WNA25_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system YbgF family protein"
                     /protein_id="HFA9824356.1"
                     /translation="MKTKLPLFIIWLSVSASCASVLPVPEGSRTEMPTQENASDGIPY
                     PVPTLQDRLDYLEGKIVRLSNEVEMLNGKVKALEHTKIHPSGRTYVQKLDDRKLKEHY
                     LNTEGGSASAHTVETAQNLYNQALKHYQNGRFSAAAALLKGADGGDGGSIAQRSMYLL
                     LQSRARMGNCESVIEIGGRYANRFKDSPTAPEVIFKIGECQYRLQQKDIARATWRSLI
                     QTYPGSPAAKRAAAAVRKR"
     gene            complement(1447..2115)
                     /locus_tag="WNA25_001639"
     CDS             complement(1447..2115)
                     /locus_tag="WNA25_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFA9824357.1"
                     /translation="MTTHLSNVAPDLQNYLNAIGEPEHPVLTRLREKAGHHRMGKMAI
                     AREQAAVLVWLAKLIRAEKYLEIGVFTGYSSTALALALPEHGRITACDINVTFTDTAR
                     QVWNEAGVAHKISLHLQPALLTLDDLIAQGEAGSYDLALIDADKPPTPQYFERCLKLV
                     RQGGIIAIDNILLNGRVMREAAFDAPPSVKILKDFNQNLPNDTRIVPITLPVGDGLTL
                     LLKK"
     gene            complement(2126..2311)
                     /locus_tag="WNA25_001640"
     CDS             complement(2126..2311)
                     /locus_tag="WNA25_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HFA9824358.1"
                     /translation="MYHYQSEATQFLNRLIEEKPELAQERLKNRGLLWDVELNPEEQK
                     NFESAKVAKKPYTYYQD"
     gene            2455..4431
                     /gene="mutL"
                     /locus_tag="WNA25_001641"
     CDS             2455..4431
                     /gene="mutL"
                     /locus_tag="WNA25_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFA9824359.1"
                     /translation="MPRIAALPDHLVNQIAAGEVVERPANALKEIVENSIDAGATAVD
                     VELEGGGIRLIRVGDNGGGIHPDDIELALHRHATSKIKTLNDLEHVASMGFRGEGLAS
                     IASVSRLTLTSRQEDSSHATQVKAEDGKLSSPTAAAHPVGTTIEAAELFFNTPARRKF
                     LKSENTEYAHCATMLERLALAHPHIAFSLKRDGKQVFKLPAQSLHERIAAIVGDDFQT
                     ASLEIDSGNSALRLYGAIAKPTFAKGKTDKQYCFVNHRFVRDKVMLHAVKQAYRDVLH
                     NALTPAFVLFLELPPKAVDVNVHPTKTEIRFRDSRQVHQLVFHTLNKALADTRANLTE
                     SVSNAGEVLHDITGVTPAPMPSENDSENLFDSASNHPTGNKPDTRNAFGSSGKTAPMP
                     YQAARAPQQHSLSLRESRAAMDTYAELYKKTDDIDLELSQFEQARFGNMPSETPAHKT
                     DTPLSDGIPSQSELPPLGFAIAQLLGIYILAQAEDSLLLIDMHAAAERVNYEKMKRQR
                     QENGNLQSQHLLIPVTFAASHEECAALADHAETLAGFGLELSDMGGNTLAVRAAPVML
                     GKSDVVSLARDVLGELAQVGSSQTIASHENRILATMSCHGSIRAGRRLTLPEMNALLR
                     DMENTPRSNQCNHGRPTWVKLTLKELDTLFLRGQ"
     gene            4526..6649
                     /gene="dnaX"
                     /locus_tag="WNA25_001642"
     CDS             4526..6649
                     /gene="dnaX"
                     /locus_tag="WNA25_001642"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFA9824360.1"
                     /translation="MTYQVLARKWRPKTFSDLVGQEHVVKALQNALDEGRLHHAYLLT
                     GTRGVGKTTIARILAKSLNCENAQHGEPCGVCQSCTQIDAGRYVDLLEIDAASNTGID
                     NIREVLENAQYAPTAGKYKVYIIDEVHMLSKSAFNAMLKTLEEPPEHVKFILATTDPH
                     KVPVTVLSRCLQFVLRNMTAQQVADHLAHVLDSEKIAYDPPALQLLGRAAAGSMRDAL
                     SLLDQAIALGSGKVAENDVRQMIGAVDKQYLYELLTGIVNQDGEALLAKAQEMAACAV
                     GFDNALGELAILLQQLALIQAVPSALAHDDPDSDILHRLAQTISGEQIQLYYQIAVHG
                     KRDLGLAPDEYAGFMMTLLRMLAFAPLAAASCDANAVIENTELQSPSAQTAEKETAAK
                     KPQPRPEADAAQTPVQTASAAAMPSEGKTAGPVSHQENNDVPPWEDAPDKTETAAGTA
                     RTSAKSIQTASEAETPPENQVSKNKAADNETEASLSEVPSENPIQATPNDEAVETETF
                     AHEAPAEPFYGFPDNDCPPEDGVEIPPPDWANVLPADTAGGGTDEEAEAGGIGGNNTP
                     SAPPPEFSTENWAAIVRHFARKLGAAQMPAQHSAWTEYRSDTGLMVLAMTAEARATAD
                     KKRLDKIRDTLAQAYGLQLTLQTEDWRDEAGRETPAMQDKRVQAEDRQKAQALLEADP
                     AAQKILQAFGAQWQPESLELAANRP"
     misc_feature    5715..5754
                     /gene="dnaX"
                     /locus_tag="WNA25_001642"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01843"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="This ribosomal frameshift element is known only in
                     the genus Neisseria; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01843"
     gene            6730..7065
                     /locus_tag="WNA25_001643"
     CDS             6730..7065
                     /locus_tag="WNA25_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFA9824361.1"
                     /translation="MFGKAGLGGLMKQAQQMQENMKKAQAKLAETEIEGEAGNGLVKI
                     TMTCAHEVRKIDISPDLIQEAADDKEMLEDLILAALKSARGKAEETANKTMGAFTQDL
                     PPGVGDFFR"
     gene            complement(7718..8764)
                     /gene="recA"
                     /locus_tag="WNA25_001644"
     CDS             complement(7718..8764)
                     /gene="recA"
                     /locus_tag="WNA25_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFA9824362.1"
                     /translation="MSDDKSKALAAALAQIEKSFGKGAIMKMDGSQQEENLEVISTGS
                     LGLDLALGVGGLPRGRIVEIFGPESSGKTTLCLEAVAQCQKNGGVCAFVDAEHAFDPV
                     YARKLGVKVEELYLSQPDTGEQALEICDTLVRSGGIDMVVVDSVAALVPKAEIEGDMG
                     DSHVGLQARLMSQALRKLTGHIKKTNTLVVFINQIRMKIGVMFGSPETTTGGNALKFY
                     SSVRLDIRRTGSIKKGEEVLGNETRVKVIKNKVAPPFRQAEFDILYGEGISWEGELID
                     IGVKNDIINKSGAWYSYNGAKIGQGKDNVRVWLKENPEISDEIDAKIRALNGVEMHIT
                     EGTQDETDGERPEE"
     gene            8964..9728
                     /locus_tag="WNA25_001645"
     CDS             8964..9728
                     /locus_tag="WNA25_001645"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFA9824363.1"
                     /translation="MHTSLTVKNTVIGSGRTKIAVPLVARDAADLSSVLSQIKNLPFD
                     IVEFRADFLECAGSIGEVLRHTQAVRDALPDKPLLFTFRRHCEGGSFPCSDDYYFELL
                     DALIESRLPDIIDIELFSGETAVRRAVANAQKNGIAALLCNHEFHRTPPQEEIVCRLK
                     QMEDCGADICKIAVMPQSSEDVLTLLSATLEAKRLVAKPVITMSMGQTGAVSRLAGQV
                     FGSSITFGSGTQNSAPGQIGVSALRAALDCLESGAD"
     gene            9748..11763
                     /gene="rep"
                     /locus_tag="WNA25_001646"
     CDS             9748..11763
                     /gene="rep"
                     /locus_tag="WNA25_001646"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFA9824364.1"
                     /translation="MMKLNPQQLEAVRYLGGPLFVLAGAGSGKTGVITQKIKHLIVNV
                     GYLPHTVAAITFTNKAAAEMQERVAKMLPKPQTRGLTICTFHSLGMKILREEANHIGY
                     KKNFSILDSTDSAKIIGELLGGTGKEAVFKAQHQISLWKNDLKTPEDVVQTASNVWEQ
                     QTARVYASYQETLQSYQAVDFDDLIRLPAVLLQQNSEVRNKWQRRLRYLLVDECQDTN
                     TCQFALMKLLTGAEGMFTAVGDDDQSIYAWRGANMENLRKMQENYPQMKVIKLEQNYR
                     STARILKIANKVIENNPKLFTKKLWSQLGEGEPVKVVACQNEQHEADWVVSQIVKQKL
                     IGGDKTRYADFAVLYRGKHQARIFEEALRGARIPYRLSGGQSFFDKAEIKDVLSYVRL
                     LANPNDDPAFLRAVTTPKRGIGDVTLGKLNAYAHEHECSLYEAAQNEEALATLNNTNR
                     QHLQAFMDMFGNYRAKAEIDEAGEFINSLLEEIDYENHLMQNEEGKAGEIKWRNVGEL
                     VSWFARKGERDGKNVIELAQTVALMTLLEGKDEEETDAVSLSTLHAAKGLEYPYVFLV
                     GCEEGVLPHNDSIEEGNVEEERRLMYVGITRAKRQLTLTHCVKRKKQGIWQFPEPSRF
                     IDEMPQEDLKILGRKGGEPIVSKEEGRRNLADIIGRIDNLKKSGPAD"
     gene            complement(11833..12891)
                     /gene="dinB"
                     /locus_tag="WNA25_001647"
     CDS             complement(11833..12891)
                     /gene="dinB"
                     /locus_tag="WNA25_001647"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFA9824365.1"
                     /translation="MSLRKIIHIDMDAFYASVELREQPHLKGRPVVVAWEGARSVICA
                     ASYEARQFGLHSAMSVATVKRLCPQAVYVPPHFDLYRQVSAQIHAVFRRYTDLIEPLS
                     PDEAYLDVTRNFKNIPYAGEVAKEIRAAIFAETGLTASAGIAPNKFLAKIASDWRKPN
                     GQFVLPPHKVMAFLETLPLGKIPGAGKVTLKKMQSLGMRTAGDLRRFERGELLNHFGR
                     YGYRLYDLARGTDEHPVKAERERLQISTEITLPEDLPLGQAAGHLPHLAEDLWRQITR
                     KNVEAQSVTLKLKTYDFRIITRTLTYSSVLPDCAALLQAAQMLMARVPPQTEDAFRLI
                     GIGVGRLVPKNQQQDLWA"
BASE COUNT         3244 a         3841 c         3425 g         2710 t
ORIGIN      
        1 ctgtctgcgg cttcgtcgcc ttgtcctgat ttaaatttaa tccactatat atttcaaaaa
       61 ttgaaacccg cacagattgt taaaccccaa aacaggggcg ggcgaaattc ttccggcaat
      121 gggttcatgc aaacaaaatg ccatctgaac cccgaaaaca gggcttcaga cggcatttgt
      181 gtattcgcgg acggcgggtt ttaaatctgt tccaattcca tgtttaaaac ggtttgttcc
      241 tgtttggctc ggaagtctgc cagtttttgc gccagttcgg ggttttcgtt ggcgagcatg
      301 gagatggcga acaatgcggc atttgccgcg cctgcctcgc cgatggcgaa tgtggcgacg
      361 ggtacgcctt tgggcatttg tacaatcgat aaaagcgaat cttcgccgcg caggtatttg
      421 cttgggacgg gtacgcccaa aacggggacg gtggtcttgg cggcaaccat gccgggtaaa
      481 tgcgccgcgc cgcccgcacc cgcgatgatg gctttgatgc cgcgcgcccg tgcggtttcg
      541 gcgtattcaa acatcaaatc cggagtgcgg tgtgcggaaa cgacgcgcgc ctcatattct
      601 acgccgaact cttcaagaaa ctgcgctgcc tgccgcataa cgggccaatc gctgttgctg
      661 cccatgatga tgccgatttg tatcataaat ccttcttggt gcggatgggg taaaaagcgg
      721 aaaaatggaa aaactatcgt ttgcgtacgg ctgcggcggc gcgttttgcc gccgggctgc
      781 cgggataggt ctgtatcagg ctgcgccaag tcgcccttgc aatgtctttt tgctgaagcc
      841 tgtattggca ttcgccgatt ttgaatatga cttcgggcgc ggttgggctg tctttgaaac
      901 ggttggcgta acgccctccg atttcgatga cagattcaca gttccccata cgcgccctgc
      961 tttgcagcaa caggtacata ctgcgttgcg cgatgctgcc gccgtctccg ccgtccgccc
     1021 ccttcaacaa ggcggctgcg gcagaaaacc tgccgttttg atagtgtttg agtgcctgat
     1081 tgtagaggtt ttgtgcggtt tcgacggtat gtgcggatgc gctgccgcct tcggtattga
     1141 ggtaatgctc tttcaatttg cggtcgtcga gtttttggac gtatgtcctg ccggaagggt
     1201 gtattttcgt atgctccaat gctttgactt tcccgtttaa catttccact tcgttcgaca
     1261 gccggacgat tttgccttcc agatagtcca aacggtcttg cagagtggga acgggatagg
     1321 gaatgccgtc tgaagcattt tcctgtgtcg gcatttcggt tcggctgccc tccggaacgg
     1381 gtaaaacgga agcacaggag gcagacacag acagccaaat gataaaaagc ggtaatttgg
     1441 tcttcattat tttttcagaa gcagggtcaa accgtcgccg acgggcaggg tgatggggac
     1501 aatccgcgta tcgttcggca ggttttgatt gaaatctttg agaattttga cactgggcgg
     1561 cgcatcaaaa gccgcttcgc gcatcaccct tccgttcaac aaaatattgt cgatggcgat
     1621 gatgccgcct tgccggacga gtttgaggca acgctcgaaa tattgcggcg tgggcggttt
     1681 gtctgcgtct atcagtgcca aatcgtagct tccggcttca ccctgtgcaa tcaaatcatc
     1741 caatgtcagc aatgcgggtt gcaggtgcag gctgatttta tgcgccacac cggcctcgtt
     1801 ccaaacctga cgcgccgtat cggtaaaggt tacattgatg tcgcaggcgg taatccgccc
     1861 gtgttcgggc agcgccaatg caagcgcggt gctgctgtat ccggtaaata cgccgatttc
     1921 cagatatttt tccgcgcgaa tcagctttgc cagccaaacc aaaaccgccg cctgttcgcg
     1981 cgcaatcgcc attttgccca tacggtgatg cccggccttc tcacgcagcc gcgtcaaaac
     2041 gggatgttcg ggttcgccga tggcattcaa atagttttgc aggtccggtg cgacattgga
     2101 cagatgggtc gtcatttagg cgggttcagt cttggtaata ggtataaggt tttttcgcca
     2161 cttttgccga ttcaaagttt ttttgctctt cgggattgag ttcgacatcc cacaaaagcc
     2221 ctcgattttt caaacgttct tgcgccaatt caggtttttc ttcgatcagg cggttgagga
     2281 attgtgtggc ttcggattgg tagtggtaca tctttgcgct ccaattttac gggatacggc
     2341 gtgattatac tggtattttc caaacgggat aaacggcttt tatcaagaat acgggcagaa
     2401 agataagggg ttttattata gaataaggcg ttttttgcaa cggaagaccg ccttatgcca
     2461 cgcattgccg ccctgcccga ccacctcgtc aaccaaattg ccgccggcga agtggtcgaa
     2521 cgccctgcca acgccctgaa agaaatcgtt gaaaacagca tagacgcggg cgcaacggcg
     2581 gttgatgtgg aactggaagg cggcggcatc cgcctcatcc gcgtcggcga caacggcggc
     2641 ggcatccacc ccgacgacat cgaactcgcg ctccaccgcc acgccaccag caaaatcaaa
     2701 accctgaacg atttggaaca cgttgccagc atgggctttc gcggcgaagg cttggccagt
     2761 atcgcctccg tcagccgcct gaccctgacc agccgccaag aagacagttc gcatgcgacc
     2821 caagtcaaag ccgaagacgg caaactcagc agccccaccg ccgccgccca ccccgtcggc
     2881 accaccatcg aagccgccga actcttcttc aacacccccg cgcggcgcaa gttcctcaaa
     2941 tccgaaaaca ccgaatacgc tcactgcgcc accatgctcg aacgcctcgc gctggcgcat
     3001 ccgcacattg ccttctcact caaacgcgac ggcaaacaag tgttcaaact tcctgcccaa
     3061 agcctgcatg aacgtatcgc cgccattgtc ggcgatgatt ttcagacggc atctttggaa
     3121 attgacagcg gcaatagcgc gctgcggctc tatggcgcga ttgccaagcc gactttcgcc
     3181 aaaggcaaaa ccgataaaca atattgcttc gtcaaccatc gttttgtccg cgacaaagtc
     3241 atgctccatg ccgtcaagca ggcataccgc gacgtattgc acaacgccct cacccctgct
     3301 ttcgtccttt tcctcgagct gccgcccaaa gccgtggatg tcaacgtcca cccgaccaaa
     3361 accgaaatcc gcttccgcga cagccggcaa gtacaccaac tcgtgttcca cacgctcaac
     3421 aaagcccttg ccgacacacg cgccaacctg accgaaagcg tcagcaacgc aggcgaagtg
     3481 ttgcatgaca ttaccggcgt tacgcctgcc ccaatgccgt ctgaaaacga cagcgaaaat
     3541 ctgtttgata gcgcatccaa ccacccgaca ggcaacaaac ccgatacacg caatgccttt
     3601 ggttcatcag gtaaaaccgc gcccatgcct taccaggccg cgcgtgcgcc gcaacaacac
     3661 agcctgtccc tgcgcgaaag ccgcgcggca atggacacct atgccgaact ctacaaaaaa
     3721 accgacgaca tcgaccttga gttgagccaa ttcgaacagg cacgtttcgg caatatgccg
     3781 tctgaaacgc ctgctcacaa aacagatacg ccgctttcag acggcatccc gtcccaatcc
     3841 gaactgccgc cgctcggttt cgccattgcc caattacttg gcatctacat tcttgcccaa
     3901 gccgaagaca gcctgttgct catcgatatg cacgccgctg ccgaacgcgt caactacgag
     3961 aaaatgaaac gccaacgtca ggaaaacggc aacctgcaaa gccagcacct gcttattccc
     4021 gtaacctttg ccgcgtccca cgaagaatgc gccgccctcg ccgatcatgc cgaaacgctg
     4081 gcaggcttcg gcctggaact gtccgacatg ggcggcaaca ccctcgccgt ccgcgccgcg
     4141 cccgtaatgt tgggcaaatc cgatgtcgtc tccctcgcac gcgacgtatt gggcgaactc
     4201 gcccaagtcg gcagcagcca aaccatcgca tcacacgaaa accgcatcct cgccacaatg
     4261 tcctgccacg gctcaatccg cgccggccgc aggctcaccc tgcccgaaat gaacgcgctg
     4321 ctgcgcgata tggaaaatac gccgcgcagc aaccagtgca accacggcag gccgacgtgg
     4381 gtcaaactga ctttgaaaga attggacaca ctgttcttgc gcggacagta agccgaaaat
     4441 gctagaatac gccgcccgaa gccgccgttc agacggcatt ccgacgcacc gacagaaaca
     4501 tcacgaccga aaccaagaga aaaacatgac ctatcaagtt ctcgcccgaa aatggcggcc
     4561 caaaaccttt tccgacttag tcggtcagga acacgtcgtc aaagccctgc aaaatgcctt
     4621 ggacgaaggc aggctgcacc acgcctacct gctgaccggg acgcgcggcg tgggcaaaac
     4681 caccatcgcc cgcatccttg ccaaaagcct caactgcgaa aatgcgcaac acggcgaacc
     4741 ttgcggcgtg tgccaaagct gcacccaaat cgatgccgga cgctatgtcg acctgctcga
     4801 aatcgacgcc gcctccaaca caggcatcga caacatccgc gaagtcttgg aaaacgccca
     4861 atacgcgccg accgccggca aatacaaagt ctatatcatc gacgaagtgc atatgctttc
     4921 caaaagcgcg ttcaacgcca tgctcaaaac gctggaagag ccgcccgaac acgtcaaatt
     4981 catcctcgcc accaccgatc cgcacaaagt ccccgttacc gtattaagcc gctgcctgca
     5041 attcgtctta cgcaatatga ccgcgcaaca ggttgccgac caccttgccc acgtcctcga
     5101 cagcgaaaaa atcgcctacg acccccccgc cctgcaactt ttgggacgtg ccgccgccgg
     5161 atcgatgcgc gatgccttaa gcctgctcga ccaagccatc gccctaggtt cgggcaaagt
     5221 tgccgaaaac gatgtccgcc aaatgatcgg cgcggttgac aaacaatacc tgtacgaact
     5281 gctgacaggc atcgtcaacc aagacggcga agccctgctg gcaaaagcgc aggaaatggc
     5341 ggcgtgtgcc gtcggctttg acaacgcctt gggcgaactg gccatactgc tgcaacaact
     5401 cgccctgata caggcagtcc cctccgcctt ggcgcacgac gaccccgatt ccgatatttt
     5461 gcaccgcctc gcccaaacca taagcggcga acaaatccag ctttactacc aaatcgccgt
     5521 ccacggcaaa cgcgacctgg gcctcgcccc cgacgaatac gccggcttta tgatgaccct
     5581 gctgcgtatg ctggcgttcg cccccttggc ggcggcttcg tgtgatgcaa acgccgtgat
     5641 tgaaaatacc gaacttcaat ccccgtcggc acaaaccgcc gaaaaggaaa ccgccgcaaa
     5701 aaagccacaa ccgcgccctg aagcggatgc cgcccaaacg cccgttcaga cggcatccgc
     5761 agcagcaatg ccgtctgaag gcaaaactgc cggccccgtt tcccatcaag aaaacaacga
     5821 tgttccgcct tgggaagacg cgccggacaa aaccgaaacc gcagccggca cggcgcgaac
     5881 gtcggcaaaa agcattcaga cggcatccga agccgaaacg ccgcccgaaa accaagtttc
     5941 caagaacaag gcagccgaca acgaaaccga ggcttccttg tccgaagtgc cgtctgaaaa
     6001 ccccattcag gcaacaccga atgatgaagc cgttgaaacg gaaacatttg cacacgaagc
     6061 ccctgcagaa cctttctacg gttttccgga taatgactgc cccccggaag acggcgtgga
     6121 aatcccgccg cccgattggg caaatgtcct ccctgccgat accgcaggcg gagggacgga
     6181 cgaagaagca gaagcgggcg gcatcggcgg aaacaacacg ccgtccgccc cgccgcccga
     6241 attttccacc gaaaactggg cagccatcgt ccggcacttc gcccgcaaac tcggcgcggc
     6301 gcaaatgccg gcgcaacact ccgcgtggac ggaataccgt tccgacaccg gactgatggt
     6361 tttggcaatg accgccgaag cccgcgccac cgccgacaaa aagcggctcg acaaaatccg
     6421 cgacaccctc gcccaagcct acgggctgca actcaccctg caaaccgaag actggcgcga
     6481 cgaagccggc cgggaaaccc ccgcgatgca ggacaagcgt gtccaagccg aagacaggca
     6541 aaaagcacaa gcattgctcg aagccgaccc cgccgcacaa aaaatcctcc aagcattcgg
     6601 cgcgcaatgg cagcccgaat cactggaatt ggcggcaaac cggccataaa cagatataat
     6661 gccgcccgaa cccttcggac ggcattgccg cttccctcat tcattcaaac acaaacagga
     6721 gtattcagta tgttcggaaa agccggatta ggcggcctga tgaaacaggc gcagcaaatg
     6781 caggaaaaca tgaaaaaggc acaagccaaa cttgccgaaa ccgaaatcga aggcgaagca
     6841 ggcaacggct tggtcaaaat cacaatgacc tgcgcgcacg aagtacgcaa aatcgacatc
     6901 agccccgatt tgattcaaga agccgccgat gacaaagaaa tgctcgaaga cctcatcctc
     6961 gccgccctca aatccgcccg gggcaaagcc gaagaaaccg caaacaaaac aatgggcgca
     7021 ttcacccaag atctgccccc cggcgtgggc gacttcttcc gctgaccccg accgtcattc
     7081 ccgcgaacgc gggaatccgg taaccgaaaa accacaggaa tctatcggaa aaaaacagaa
     7141 acccccgccg ccgtcattcc cgcgcaggcg ggaatctaga acgtagaatc tgaaaaaccg
     7201 ttttactcga taaatttccg tgccgacagg tctggattcc cgccttcgcg ggaatgacgg
     7261 catatttttt gcatttgata taaagggtcg tttgaatttt gttcagcaag tgcaaagtat
     7321 tgcacataaa agggcgcagg cttcatccgt aaagtcggcg caggatagag gcaaagcggg
     7381 cgtaggtcgg gctgtagcaa ctgtattttt caccccgtcg ggtaaaaata tagtggatta
     7441 acaaaaaccg gtacggcgtt gcctcgcctt agatcaaaga gaacgattct ctaagatgct
     7501 gaagcaccaa gtgaatcggt tccgtactat agtggattaa atttaaacca gtacggcgtt
     7561 gcctcgcctt gccgtactat ttgtactgtc tgcggctctc cgccttgtcc tgatttttgt
     7621 taatccgcta taccaaaact caaatcaagc cgtccggagg cggctcaaaa aaaacggtac
     7681 ttcgcagcag aagtaccgtt tatcgggatt tcaggtttta ttcttcgggt cgttcgccgt
     7741 cggtttcgtc ctgcgtccct tcggtgatgt gcatttctac gccgttgagg gcgcggattt
     7801 ttgcgtcgat ttcgtcggaa atttcggggt tttctttcag ccagacgcgg acgttgtctt
     7861 tgccctgacc gattttcgcg ccgttgtagc tgtaccacgc gccggatttg ttgatgatgt
     7921 cgtttttcac gccgatgtcg atcaattcgc cttcccaact gatgccttct ccgtagagga
     7981 tgtcaaactc tgcctgacgg aacggggggg cgactttgtt tttgatgact ttgacgcggg
     8041 tttcgttgcc caatacttct tcgccttttt tgatggatcc ggtgcggcgg atgtcgaggc
     8101 ggacggaaga atagaatttc agcgcgttgc cgccggtcgt ggtctcgggg ctgccgaaca
     8161 ttacgccgat cttcattcgg atttggttga tgaacacaac cagcgtattg gtttttttga
     8221 tgtgtccggt cagtttgcgc aaagcctgac tcatcaggcg cgcctgcagt ccgacgtggc
     8281 tgtcccccat atcgccttcg atttcggctt tggggacgag tgcggctacg gaatcgacga
     8341 ctaccatatc tatgccgccc gaacggacga gcgtgtcgca gatttccaaa gcctgttcgc
     8401 cggtatcggg ctgggacagg taaagctctt cgacttttac gccgagtttg cgggcgtaaa
     8461 cgggatcgaa ggcgtgttcg gcatcgacaa aggcgcacac gccgccgttt ttctggcatt
     8521 gggcgacggc ttcgaggcag agtgtggttt tgccggagga ttcggggccg aagatttcga
     8581 cgatgcgccc gcgcggcagg ccgccgactc cgagggcgag gtctaatccg agcgatccgg
     8641 tggaaatgac ttcgaggttt tcttcctgct ggctgccgtc cattttcatg atggcgcctt
     8701 tgccgaaact tttttcgatt tgcgccagtg cggcggcaag ggctttgctt ttgtcgtctg
     8761 acatgggggt tactccggaa caaatgcggt atgtgggctg cggcgcaaca cgggctgcgg
     8821 cgcggaatat gaatcggtcg gtcattatcg catgtttgcc tttcgggggc taccatccgc
     8881 ttcagaccga atgccgtctg aaacattgtc cctcctttgc tataatggcg cccttatttt
     8941 tcccgccagc cgcttgaacc gccatgcaca ccagcctgac cgtcaaaaat accgttatcg
     9001 gaagcgggcg caccaaaatc gccgtgccgc ttgtcgcccg agatgccgcc gatttatcct
     9061 ccgtgctgtc ccaaatcaaa aacctgccct tcgatatcgt cgagttccgc gccgactttt
     9121 tggaatgcgc gggcagcatc ggcgaagtat tgcgccacac gcaggccgtc cgcgacgcgc
     9181 tgcccgacaa accgctgctg tttacgttca gacggcattg cgaaggcggc tcgttcccgt
     9241 gttcggacga ttattatttt gaactgctcg acgcgctgat cgaaagccgc ctgcccgaca
     9301 ttatcgatat cgagctgttt tccggcgaaa ccgccgtccg ccgcgccgtg gcaaatgctc
     9361 aaaaaaacgg catcgccgcc ctgctctgca atcatgagtt tcaccgcacg ccgccgcaag
     9421 aagaaatcgt atgccgtctg aaacagatgg aggactgcgg cgcggacatc tgcaaaattg
     9481 cggtgatgcc gcaaagctcg gaagatgtgc tgactttgct ttccgccacg ctcgaagcga
     9541 aacggcttgt cgccaaaccg gttattacga tgtcgatggg gcagacgggt gcggtcagcc
     9601 ggcttgccgg acaggtgttc ggctcaagca tcacgttcgg ttcgggaacg caaaactccg
     9661 cgccggggca aatcggcgta tccgccctcc gtgcggcact cgactgcctc gaaagcggcg
     9721 cagactgatt tcagacggca tcaaaacatg atgaaactca atccccaaca gctcgaagcc
     9781 gtccgctacc tcggcggccc cctgttcgtc cttgccggcg caggcagcgg caaaaccggc
     9841 gtgattacgc aaaaaatcaa gcatttgatt gtcaatgtcg gctatctgcc gcataccgtc
     9901 gccgcgatta cctttaccaa caaagccgct gcggaaatgc aggagcgcgt cgccaaaatg
     9961 ctgcccaaac cgcaaacgcg cgggctgacg atttgcacgt tccattcttt gggcatgaag
    10021 attctgcgcg aagaggcgaa ccatatcggt tacaaaaaaa acttctccat tctcgactct
    10081 accgacagcg ccaaaatcat cggcgaactc ttaggcggca cgggaaaaga agccgtattc
    10141 aaggcgcagc accagatttc cctttggaaa aacgatttaa aaacgcctga agatgtcgtt
    10201 cagacggcct ctaatgtgtg ggaacaacaa accgcgcgcg tgtatgcaag ttatcaggaa
    10261 accctgcaaa gctatcaggc agtagatttt gacgacttaa tccgcctgcc tgccgtgctg
    10321 ttgcagcaaa acagtgaagt gcgcaacaaa tggcagcggc ggctgcgtta tctgttggtt
    10381 gacgaatgcc aagatacgaa tacctgccaa tttgcgctga tgaaactctt gacaggcgcg
    10441 gaagggatgt ttaccgccgt cggcgacgac gaccagtcca tctacgcatg gcgcggcgcg
    10501 aacatggaaa acctgcgcaa aatgcaggaa aactatccgc agatgaaggt catcaaactg
    10561 gagcaaaact accgctccac cgcgcggatt ctcaaaattg ccaacaaagt catcgaaaac
    10621 aaccccaagc tgtttaccaa aaaactttgg tcgcaattgg gcgaaggcga gccggttaaa
    10681 gtcgttgcct gccaaaacga gcagcacgaa gccgattggg tcgtcagcca aatcgtcaaa
    10741 caaaaactca tcggcggcga caaaacccga tacgccgatt ttgccgtgtt ataccgcggc
    10801 aagcatcagg cacggatttt cgaggaagca ttgcgcggcg cgcgcatccc ctaccgtctc
    10861 tccggcggac aaagcttttt cgacaaagcc gaaatcaaag acgtgttgtc ttatgtgcgg
    10921 ctgcttgcca accccaacga cgatcccgcc tttctgcgcg ccgttaccac gccaaaacgc
    10981 ggcatcggcg atgtaacgct gggcaaactc aacgcctacg cgcacgagca cgaatgcagc
    11041 ctgtatgaag ccgcacaaaa cgaagaagcc cttgccacac tgaacaatac caaccgccaa
    11101 cacctgcaag cctttatgga tatgttcggc aactaccgcg ccaaagccga aatcgacgaa
    11161 gcgggcgagt tcatcaacag cctgctcgaa gaaatcgact atgaaaacca tttgatgcaa
    11221 aacgaagaag gcaaagccgg cgaaatcaaa tggcgcaacg tcggcgagtt ggtatcgtgg
    11281 ttcgcacgaa aaggcgaacg ggacggcaaa aacgtcatcg aactcgccca aaccgtcgcc
    11341 ttgatgacgc ttttggaagg aaaagacgaa gaagaaaccg atgccgtctc gctatccacg
    11401 ctgcacgccg ccaaaggttt ggagtatccg tatgttttcc ttgtcggttg cgaagaaggt
    11461 gttttgccgc acaacgacag catcgaagaa ggcaacgtcg aagaagaacg ccgcctgatg
    11521 tacgtcggca tcacccgtgc caaacgccaa ctcacgctga cccactgtgt caaacgcaaa
    11581 aaacaaggca tatggcaatt ccccgaaccc agccgattca tagacgaaat gccgcaggaa
    11641 gatttgaaaa tcctggggcg caaaggcggc gaaccgattg tcagcaaaga agaaggcaga
    11701 cgtaaccttg ccgatataat cggaaggatc gacaacctaa aaaaaagcgg cccggcggat
    11761 taaaccggag ccgcaatgcc gtctgaaaga cgttcagacg gcatattttt tgaacggcgc
    11821 gcgtcaagcg gtttacgccc acaaatcctg ctgctgattt ttcggtacaa gcctgccgac
    11881 accgataccg atgaggcgga acgcgtcttc cgtctgcggc gggacgcgcg ccatcaacat
    11941 ttgcgcagcc tgcagcagag ctgcgcagtc gggcaatacg gaggaataag tcagcgtgcg
    12001 cgtgatgatg cggaaatcgt aggtcttcag cttgagcgtt acgctttggg cttcgacgtt
    12061 tttgcgcgtg atttgccgcc acaggtcttc ggcaagatgg ggcaggtgtc cggcagcctg
    12121 cccgagcggc aggtcttcgg gcagggtgat ttctgtggaa atttggaggc gttcgcgttc
    12181 ggctttgacg gggtgttcgt ccgtaccgcg cgccaaatca tagaggcggt agccgtagcg
    12241 tccgaaatga tttaagagct cgccgcgctc gaaacggcgc aaatcgcccg ccgtccgcat
    12301 acccagcgac tgcatttttt tcagcgttac cttgcccgct ccggggattt tgcccaaagg
    12361 cagggtttcc aaaaatgcca taactttgtg cggcggcaac acaaactgcc cgttcggctt
    12421 gcgccagtcg gaagcaattt tcgccagaaa tttgttcggc gcgatgcctg cggatgcggt
    12481 caaacccgtt tccgcaaaaa tggcggcacg gatttctttg gcaacttcgc cggcgtaagg
    12541 gatatttttg aaattacggg taacgtcaag ataggcttcg tccggcgaca agggttcgat
    12601 taaatcggta taacgcctga atacggcgtg aatctgcgcg gaaacctgac ggtacaaatc
    12661 gaaatgcgga ggcacataca ccgcttgcgg acacagcctt ttcaccgttg ccaccgacat
    12721 cgcggaatgc agcccgaact gccgtgcctc atacgatgcg gcgcaaatca ccgaacgcgc
    12781 gccctcccac gcgacgacca ccggccgccc tttcaaatgc ggctgctcgc gtagttctac
    12841 cgatgcgtag aacgcgtcca tgtcgatgtg gatgattttg cgtaaagaca tcggctctgc
    12901 cgaggataaa agggatattt tactgcgggc atcggacaaa atccaaatat actaacaggt
    12961 taaatacaaa agagttggct ggattaattt tgaaactcga taaaaataat ggtttttatc
    13021 aagcagaaag aaacagtaat ggaaattatg atatcgtaag aaaaaattat ctgaaaaata
    13081 ttaccaaaat acaatcaaat atattgattt acttagataa taccagtacc ggatttaaaa
    13141 ttcaacagag gagaaaacaa atcagagcac aaatttcagc cagacaatgg agaagataaa
    13201 agatgaataa aaatataatt
//
LOCUS       DAWTQS010000058        13123 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.058, whole genome shotgun sequence.
ACCESSION   DAWTQS010000058 DAWTQS010000000
VERSION     DAWTQS010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13123
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.058"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            <1..1264
                     /locus_tag="WNA25_001648"
     CDS             <1..1264
                     /locus_tag="WNA25_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFA9824366.1"
                     /translation="YSPKKYAVALGSSSTASGEYSYASGYNSVASGNKSYAAGYASVA
                     SAEGSVVIGDSRQVKPEADQGVAVGSKATVKNKAKQRVAVGSEAKVNAERGIAIGKEA
                     KAGGKTTNTLLDGPAYYADAIAVGYQAEAGKGGAIALGKQAKATKQNGMALGVESEAA
                     GDFSTAVGNESKAKGQGGVGLGNQSKAEADFAVAVGNKAEATKENSLVIGRYARANGN
                     HSVSLGSRSEIKDGVSNSVAPGYGSVASENNVVSVAYKETPQSTELSYRKIVGVDDGV
                     NDFDAVNVRQLKAMQGQNMAELFSVRSEVRGVAASSAALSALTPLSYDANNPTQFMVG
                     FGTYKGRQAMALGLSHFVNDRFMVKVGGTLGGNKTGHMMNVGLTWKFGTSQGVPATSD
                     KVLMEQLMQDNRQLKARLEKLEARLNAM"
     gene            complement(1403..1918)
                     /gene="coaD"
                     /locus_tag="WNA25_001649"
     CDS             complement(1403..1918)
                     /gene="coaD"
                     /locus_tag="WNA25_001649"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFA9824367.1"
                     /translation="MLPNTPRRAVYAGSFDPPTLGHLWMIRQAQSMFDELIVAIGINP
                     DKRNTYTAAERQDMLCAITDNFPNVRIEVFQNRFLVHYAREVDAGFIVRGIRSTSDYE
                     YERSMRHINSDLAPEISTVFLMPPREIAEVSSTMIKGLVGPEGWMETVKRYVPPAVYQ
                     KMIAEHHNNNA"
     gene            complement(2048..2731)
                     /locus_tag="WNA25_001650"
     CDS             complement(2048..2731)
                     /locus_tag="WNA25_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFA9824368.1"
                     /translation="MQHDVYDYTAHTVSKNTVLQKTYRLLGFSFIPAAAGAALAANAG
                     FNFYAAFGSRWIGFAVVLAFFYGMIHFIEKNRYSNTGVTLLMVFTFGMGVLIGPVLQY
                     ALHIADGAKIVGIAAAMTAAVFLTMSALARRTRLDMNALGRFLTVGAVILMVAVVANL
                     FLGIPALALTISAGFVLFSSLMIMWQVRTVIDGGEDSYISAALTLFISLYNIFSSLLN
                     ILLSLNGDD"
     gene            complement(2888..3154)
                     /locus_tag="WNA25_001651"
     CDS             complement(2888..3154)
                     /locus_tag="WNA25_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFA9824369.1"
                     /translation="MARMVFCVKLNKEAEGMKFPPLPNELGKRIFENVSQEAWAAWTR
                     HQTMLINENRLSLADPRAREYLAQQMEQYFFGDGADAVQGYVPQ"
     gene            complement(3228..3698)
                     /gene="rlmH"
                     /locus_tag="WNA25_001652"
     CDS             complement(3228..3698)
                     /gene="rlmH"
                     /locus_tag="WNA25_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFA9824370.1"
                     /translation="MNITVLAVGTKMPRWVDEAVAEYAKRFGRDAAYALKEIKPEKRG
                     AGVNAVQGMAAEEKRILEAIPQGAFLVVLDERGKAPTSVELAEHLKSWRQNGEHVCFV
                     IGGADGMTDRLKQQARMMMRLSSLTLPHGMVRVLLTEQLYRAVSILHNHPYHRE"
     gene            complement(3751..4137)
                     /gene="rsfS"
                     /locus_tag="WNA25_001653"
     CDS             complement(3751..4137)
                     /gene="rsfS"
                     /locus_tag="WNA25_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFA9824371.1"
                     /translation="MNEQELQDLQKMVGVAVNALEDIKAKDISVLETQDKTSLFARMI
                     IASGDSTRQVKALANNVAVDLKEAGFEILSTEGDSGEWTLVDAGDLVVHVMLPAVRDF
                     YDIDTIWGGEKPSFHAGMQKPWHAAD"
     gene            complement(4196..4801)
                     /gene="nadD"
                     /locus_tag="WNA25_001654"
     CDS             complement(4196..4801)
                     /gene="nadD"
                     /locus_tag="WNA25_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HFA9824372.1"
                     /translation="MKKIGLFGGTFDPIHNGHFHIARAFADEIGLDAVVFLPAGGPYH
                     KDAASASAADRLAMVELATAEDARFAVSDCDIVRESATYTFDTVQIFRRQFPSAQLWW
                     LMGSDSLLKLHTWKKWQLLVRETNIAVAMRQGDSLHQTPRELHAWLGNALQDGSVRIL
                     SAPMHNVSSTEIRRNLSAAGVSDGIPPVAARYIRKHGLYEK"
     gene            complement(4798..5397)
                     /locus_tag="WNA25_001655"
     CDS             complement(4798..5397)
                     /locus_tag="WNA25_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HFA9824373.1"
                     /translation="MSELSEILSYNQKFVESGEYEKYFTDKYPERGLAVLSCMDARII
                     GLLPDALGLKNGDAKLIKNAGALVTHPWGSVMRSLLVAVFELKVREIMVIAHHDCGMQ
                     GLNAEEFLGRVRESRIPEDRIETLRYAGVDLDGWLTGFDNVEDSVRHTVDLIRNHPLM
                     PRHIAVHGLVIHPVTGKLTLVVDGSVSDGMDLSEGMETS"
     gene            complement(5426..6967)
                     /locus_tag="WNA25_001656"
     CDS             complement(5426..6967)
                     /locus_tag="WNA25_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HFA9824374.1"
                     /translation="MDGRRWAVRGAFSLLPSAFLAVMVVAPLWAVAAYDGLAWRAVLS
                     DAYMFKRLAWTVFQAAATCVLVLPLGVPVAWVLARLAFPGRALVLRLLMLPFVMPTLV
                     AGVGVLALFGADGLLWRGRQDTPYLLLYGNVFFNLPVLVRAAYQGFAQVPAARLQTAR
                     TLGAGAWRRFWDIEMPVLRPWLAGGVCLVFLYCFSGFGLALLLGGSRYATVEVEIYQL
                     VMFELDMAGASALVWLVLGVTAAAGLLYAWFGRRAVSDKAVSPVMPSPPQSVGEYVLL
                     AFSVAVLSVCCLFPLSAIVVKAWSAGESRRVLMESETWQAVWNTLRFSAAAVFAAAVL
                     GVVYAAAARRLVWMRGLVFLPFMVSPVCVSAGVLLLYPGWTASLPLLLAMYALLAYPF
                     VAKDVLSAWDALPPDYGRAAAGLGANGFQTACRITFPLLKPALRRGLTLAAATCVGEF
                     AATLFLSRPEWQTLTTLIYAYLGRAGEDNYARAMVLTLLLSAFAVCIFLLLDNGEGGK
                     RTETL"
     gene            7358..7867
                     /locus_tag="WNA25_001657"
                     /pseudo
     CDS             7358..7867
                     /locus_tag="WNA25_001657"
                     /inference="COORDINATES: protein motif:HMM:NF014500.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
     gene            7842..8744
                     /locus_tag="WNA25_001658"
     CDS             7842..8744
                     /locus_tag="WNA25_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HFA9824375.1"
                     /translation="MHVFLPPHPGPIAASEFYGANIGQVLILGLPTAFITWYFSGYML
                     GKVLGRAIHVPVPELLSGGTRDSDPPKEPAKAGTVVAVMLIPMLLIFLNTGVSALISE
                     KLVSADETWVQTAKMIGSTPVALLISVLAALLVLGRKRGESGSTLEKTVDGALAPVCS
                     VILITGAGGMFGGVLRASGIGKALADSMADLGIPVLLGCFLAALALRIAQGSATVALT
                     TAAALMAPAVAAAGFTDWQLACIVLATAAGSVGCSHFNDSGFWLVGRLLDMDVPTTLK
                     TWTANQTLIALIGFALSALLFAIV"
     gene            8764..9282
                     /locus_tag="WNA25_001659"
     CDS             8764..9282
                     /locus_tag="WNA25_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFA9824376.1"
                     /translation="MTTHFVVTGVCGCGKTTAAPSLQKHLGQCPYAEGDEFHTQANRD
                     KMGAGIPLTDEDRYPWLGNLRDWMTQQAQNGADHTIVTCSALKRGYRDILRGAEGKAA
                     FIHLSPPQDINLERMMSRKGHYMKAGMLDSQLEILEELGEGEYGVKIANPGTPEAVEA
                     DILNWVASENLL"
     gene            complement(9468..10385)
                     /gene="thrB"
                     /locus_tag="WNA25_001660"
     CDS             complement(9468..10385)
                     /gene="thrB"
                     /locus_tag="WNA25_001660"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFA9824377.1"
                     /translation="MSVYTSVSDDEMRGFLSGYDLGEFVSLQGIAQGITNSNYFLTTT
                     SGRYVLTVFEVLKQEELSFFLELNRHLSMKGVAVAAPVARKDGRLDSVLAGKPACLVA
                     CLKGSDTALPTAEQCFHTGAMLAKMHLAAADFPLEMENPRYDAWWTEACARLLPVLSQ
                     DDAALLCSEIDALKDNLGNHLPSGIIHADLFKDNVLLDGGQVSGFIDFYYACRGNFMY
                     DLAIAVNDWARTADNKLDEALKKAFIGGYEGVRPLSAGEKAYFPTAQRAGCIRFWVSR
                     LLDFHFPQAGEMTFIKDPNAFRNLLLSLD"
     gene            complement(10422..11150)
                     /gene="ubiG"
                     /locus_tag="WNA25_001661"
     CDS             complement(10422..11150)
                     /gene="ubiG"
                     /locus_tag="WNA25_001661"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFA9824378.1"
                     /translation="MGGKMSDKKYNVDEGEIAKFSRIADKWWDKSGEFKTLHDINPLR
                     LDYIDGHADLCGKRVLDVGCGGGILAESMARRGAAFVKGIDMAEQSLETARLHAALNN
                     VADIEYECIRVEDLAEAEPHSFDVVTCMEMMEHVPDPAAIVRACAKLVKPDGMVFFST
                     INKNPKSYLHLIVAAEYLLKFVPKGTHDWKKFIAPAELARMCRQAGLDVADTKGMTYH
                     VLSQTYALCDSTDVNYMFACRPAF"
     gene            11302..12543
                     /locus_tag="WNA25_001662"
     CDS             11302..12543
                     /locus_tag="WNA25_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235268.1"
                     /note="tryptophan transporter of high affinity; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFA9824379.1"
                     /translation="MPTKTPSLFGGAMIIAGTVIGAGMLANPTATSGVWFTGSLAVLL
                     YTWFSMLSSGLMILEVNTHYPHGASFDTMVKDLLGRGWNIINGIAVAFVLYLLTYAYI
                     FVGGDLTAKGIGSAVGGKISLTVGQLVFFGILAFCVWASARLVDRFTGVLIGGMVLTF
                     IWATGGLVADAKPSVLFDTQAPAGTGYWIYAATALPVCLASFGFHGNVSSLLKYFKGD
                     APKVAKSIWAGTLVALVIYVLWQTAIQGNLPRNEFAPVIAAEGQVSVLIETLSKFAQT
                     GNMDKILSLFSYMAIATSFLGVTLGLFDYIADIFKWNDSMSGRTKTAALTFLPPLISC
                     LLFPTGFVTAIGCVGLAATVWTGIIPAMLLYRSRKKFGAGKTYKVYGGLWLMVWVFLF
                     GIANIAAQVLSQMELVPVFKG"
     gene            12695..>13123
                     /locus_tag="WNA25_001663"
     CDS             12695..>13123
                     /locus_tag="WNA25_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFA9824380.1"
                     /translation="MKLKIDIATNNFKHGGGTERYTLDLVKGLNRQNITPAVYATKFD
                     HGIPEYAMIEPHLVDQRRTLKKLRSFLFSSRLAQTRKNSAAKLIACHHADYADLLICG
                     GTHLGYLHHMAQKPNLLDRLAIRRNRSNYATAKLIVAHSHM"
BASE COUNT         3097 a         3843 c         3405 g         2778 t
ORIGIN      
        1 gtacagcccc aagaaatatg cagtagcatt aggatctagt tctactgcca gtggagagta
       61 ttcctatgcg tcgggctata attctgttgc cagtggaaat aaatcctatg ctgcaggcta
      121 tgcttctgtt gccagtgcag aaggcagtgt tgtaattggc gattcaaggc aagtaaaacc
      181 ggaagcagat caaggggttg cagtaggttc taaagcaacg gtaaagaaca aagctaaaca
      241 aagggttgcg gtaggttctg aggcaaaggt aaatgcagaa cgtggtattg cgattggtaa
      301 agaagccaaa gcagggggca aaacaacgaa cacactgtta gatggaccgg catattatgc
      361 ggacgctatt gccgtcgggt atcaagcaga ggctggcaaa ggtggcgcca tcgctttggg
      421 aaaacaagca aaagcaacga agcaaaatgg aatggcactt ggcgtagaat cagaagctgc
      481 cggtgatttt agtaccgccg tgggtaatga aagcaaggca aaggggcaag gcggcgtagg
      541 tctcggtaac caatctaagg cagaggcaga ttttgcggtg gctgtgggga ataaggctga
      601 agcgactaag gaaaactcac ttgtcattgg ccgatatgca agggcaaacg gaaatcattc
      661 tgtgagttta ggatctcgta gtgaaataaa agatggtgtg agcaattctg tggcaccggg
      721 ttatggttca gtagctagtg agaataatgt cgtctctgtt gcctataaag aaacaccaca
      781 atctacagaa ctttcctata gaaaaattgt aggtgttgat gatggtgtca atgattttga
      841 tgccgtcaat gtacgccagt tgaaagcgat gcaaggacaa aatatggctg agctattttc
      901 agtacgcagc gaagtgcgtg gtgtggcagc gagtagtgca gccctatcgg ctctgactcc
      961 attatcctat gatgcaaaca atcctacgca gtttatggtc ggtttcggca catacaaagg
     1021 acgtcaagct atggcattag gattgtccca tttcgtcaat gaccgcttta tggtaaaagt
     1081 aggcggaacc ttaggcggca ataagacggg acatatgatg aatgtaggct tgacttggaa
     1141 atttggaacg agccaaggtg ttccggctac aagtgataag gtgcttatgg aacagttgat
     1201 gcaagataat cgtcaattaa aggcacgatt ggagaaatta gaagcacgtc tgaatgctat
     1261 gtagccttgt ggcgataacg gtttattgag tatatcgcca atgataaagt gcataatttt
     1321 caaaccgttg ataatataca ataaattgta accaatacct caaacgggtt tgaacccgaa
     1381 tatgcctgac aggaactttc tgtcaggcat tgttgttgtg atgttctgca atcatttttt
     1441 ggtacacggc cggcggcaca tatcttttga cggtttccat ccagccctcg ggcccgacca
     1501 gtcctttgat catagtggac gacacttcgg cgatttcgcg cggcggcatg aggaatacgg
     1561 tagatatttc gggtgcgagg tcgctgttga tatggcgcat ggagcgttcg tattcataat
     1621 cggaggtgga acggatgccg cgcacgatga atcccgcatc tacctcacgg gcgtaatgca
     1681 ccaaaaaccg gttttgaaac acttcaattc tgacgtttgg gaagttgtcg gtaatggcgc
     1741 acaacatatc ctgcctttca gcggcggtat aggtgttgcg tttgtcggga ttgatgccga
     1801 tggcgacgat gagttcgtca aacatagatt gcgcctgccg tatcatccac agatgcccca
     1861 atgtgggcgg atcgaaactg ccggcataaa cggcgcggcg cggtgtattc ggtaacattt
     1921 gattcctccc ggcttcatag ccggctgtgt ttttatgtgt ttgcatccgt attgtatgcc
     1981 caaaacaaaa tgccgtctga aaataagtgc tttcagacgg catggtttgc atggtgtttc
     2041 gcctacatca gtcgtcgccg tttaaggaca gcaggatgtt gagcaggctg ctgaagatgt
     2101 tgtaaagcga gataaacagt gtcagtgccg cgctgatgta actgtcttcg ccgccgtcga
     2161 tgacggtgcg tacctgccac ataatcatta aggaactgaa caagacaaaa ccggcggaaa
     2221 tggtcagggc gagtgcggga atacccaaaa acagattggc aaccacggcg accatcagaa
     2281 tgaccgcacc tacggtcagg aagcgtccga gcgcgttcat atcgagccgg gttcggcgtg
     2341 ccaatgcgga catcgttaaa aagacggcgg cggtcatcgc ggcggcaatg ccgacgattt
     2401 tcgcaccgtc ggcaatatgg agtgcgtatt gcagcacggg gccgatcaat acgcccatac
     2461 cgaatgtgaa taccatcagc agggtaacgc cggtattgct gtaacggttt ttttcgatga
     2521 agtggatcat accgtagaaa aacgccaata cgacggcaaa tcctatccag cgcgaaccga
     2581 aggcggcgta aaaattgaaa ccggcattgg cggcaagtgc cgcgcctgcg gctgccggaa
     2641 tgaatgaaaa tccgagcagg cggtaggttt tctgcaggac ggtgttttta gaaaccgtat
     2701 gcgcggtgta gtcgtaaacg tcgtgttgca tatcatctgc tcctgaaagc gcggttggaa
     2761 ataacggggg attttaacat tgcccaatgt caaaatttgt ccggttgcgt gaagataaag
     2821 ttgtccggcg tattttaaag gccgtctgaa gcagtttcgg acagcctgtg ttcaaaacgg
     2881 aaaaccgtta ttgcggaacg tatccctgca cggcatccgc gccgtcgccg aagaaatact
     2941 gctccatctg ctgagccagg tattcgcgcg cgcgcggatc ggcaaggctt aagcggtttt
     3001 cgttaatcag catggtttgg tggcgcgtcc acgccgccca tgcttcttgc gatacgtttt
     3061 caaaaatgcg tttgcccaat tcgttgggca gcggcggaaa tttcatgcct tcggcttctt
     3121 tgttgagctt gacgcagaat accattcgtg ccatagcgga ttcctttgct gtgttcagaa
     3181 ataacggggc gattttaacc gattaggggg tacagacaaa agccttttta ttcccgatga
     3241 taaggatggt tgtgcaggat ggaaacggcc cggtagagct gctcggtcag aaggacgcgc
     3301 accatgccgt gcggcagggt gaggctggac aggcgcatca tcatgcgggc ttgttgtttg
     3361 aggcggtcgg tcatgccgtc cgcgccgccg atgacgaagc agacgtgttc gccgttttgc
     3421 cgccagcttt tgaggtgttc cgccagctcg acggaggtcg gtgctttgcc gcgttcgtca
     3481 agaacgacga ggaacgcgcc ttgcggaatg gcttcaagga tgcgtttttc ttccgccgcc
     3541 ataccttgga cggcattcac gcccgcgccg cgtttttcgg gtttgatttc tttgagtgcg
     3601 taggcggcgt cgcgtccgaa gcgtttggcg tattcggcga cggcctcatc aacccaacgc
     3661 ggcattttgg tgccgactgc caagacggta atgttcaatg ctttccccct tacaggaaat
     3721 gccgtatgaa tattcagacg gcatcgggaa tcagtctgca gcgtgccacg gcttctgcat
     3781 tccggcgtgg aaactcggtt tctcgccgcc ccagatggtg tcaatgtcgt agaagtcgcg
     3841 cacggcaggg agcatgacgt ggacgacgag gtctcctgca tcaaccaacg tccattcgcc
     3901 gctgtctcct tcggtactga ggatttcaaa accggcttct ttcaaatcga cggcaacgtt
     3961 gttggccagt gctttgactt ggcgcgtact gtcgccgctg gcgataatca ttctggcaaa
     4021 cagcgaagtt ttgtcttgcg tttcgagaac ggaaatgtct ttggctttga tgtcttcgag
     4081 ggcattgacg gcgaccccga ccattttttg caggtcttgc agttcttgtt cgttcattgt
     4141 tttcctaacg ggatgttttc agacggcatt atagccgttc cttactgatt tgactttatt
     4201 tttcatacaa accgtgtttt cggatataac gggcggcgac aggcgggatg ccgtctgaaa
     4261 caccggcggc actcaggttg cggcggattt ccgttgacga cacattatgc atcggggctg
     4321 acaagatgcg gacgctgccg tcctgaaggg cattgcccag ccacgcgtgc agttcgcgcg
     4381 gggtttggtg caggctgtcg ccctgccgca tggcgacggc gatattggtt tcgcgcacga
     4441 gcaactgcca ttttttccaa gtatgcagct tcagcaggct gtcgctgccc atcagccacc
     4501 aaagttgcgc ggacgggaac tgccggcgga agatttggac ggtatcaaaa gtataggttg
     4561 cactttctcg gacgatgtcg cagtcgctga cggcaaaacg cgcgtcttct gccgtcgcca
     4621 gttcgaccat ggcaaggcgg tcggcggcgg aagcggaggc ggcgtctttg tgatacgggc
     4681 cgcctgccgg caggaaaaca accgcgtcca acccgatttc gtcggcaaag gcacgggcga
     4741 tatgaaaatg cccgttgtgt atcgggtcga aagtgccgcc gaacagtccg attttcttca
     4801 tgatgtttcc attccttccg ataagtccat gccgtctgaa acactgccgt ccacaaccag
     4861 cgtcagtttg ccggtaacgg gatggatgac cagtccgtga acggcgatat ggcgcggcat
     4921 cagcggatgg ttgcggataa ggtccaccgt gtggcgcacg ctgtcttcga cgttgtcgaa
     4981 accggtcagc cagccgtcga ggtcgacacc tgcataacgc agggtttcga tacggtcttc
     5041 gggaatccgg ctttcccgga cgcgcccgag gaattcttcg gcattcagcc cctgcatacc
     5101 gcaatcgtga tgggcgatga ccataatctc tctgaccttc aattcaaaca cggcaaccaa
     5161 aaggctccgc atcaccgaac cccacgggtg cgtaaccagc gcgccggcat ttttaatcag
     5221 cttggcatcg ccgtttttca aacccaacgc gtcgggcagc agcccgataa tccgcgcatc
     5281 catacaggac aaaaccgcca gcccgcgttc ggggtatttg tcggtaaagt atttttcata
     5341 ttctcccgac tcgacaaact tctgattata ggaaaggatt tcgctcaact cgctcatttt
     5401 gccgtcctct gaaaaagggt tcgcattata acgtttccgt ccgttttccg ccttcgccgt
     5461 tgtccaacag caggaaaatg cacaccgcaa atgccgacaa cagcaatgtc aacaccattg
     5521 cccgcgcata attgtcctca cccgcacgcc ccaaataggc ataaatcaaa gtcgtcaacg
     5581 tctgccattc cggacgcgac aggaacaagg ttgccgcaaa ttcgcccaca cacgtcgccg
     5641 ccgccaaagt cagaccgcgc cgcaacgccg gtttcaagag ggggaacgtg atacggcatg
     5701 ccgtctgaaa gccgtttgcg cccaaacctg ccgccgccct gccgtaatcc ggcggcagtg
     5761 catcccaggc cgataaaaca tcttttgcca caaacggata cgccagcagc gcatacatcg
     5821 ccagcagcag cggtaacgaa gccgtccacc ccggataaag cagcagcacg cccgccgaaa
     5881 cacaaaccgg cgacaccata aacggtaaaa acaccagtcc gcgcatccac accagccgcc
     5941 gcgccgccgc cgcatacacc acacccaaaa ccgccgccgc aaacaccgcc gccgccgaaa
     6001 agcgcaaagt attccacact gcctgccacg tttcactttc cattaacaca cgccgcgatt
     6061 cgccggccga ccacgctttc acaacaattg ccgacaaagg aaacaggcag cacacggaca
     6121 acaccgccac cgaaaatgcc agcaatacat attcccccac cgattgaggc ggcgacggca
     6181 tcacggggga aaccgcctta tccgaaaccg cgcgcctgcc gaaccacgca tacagcaacc
     6241 ctgccgccgc cgttaccccc aacaccagcc acaccagcgc cgaagccccc gccatatcga
     6301 gttcgaacat aaccaactgg taaatttcca cttcgaccgt ggcataacgg ctgccgccca
     6361 acagcaatgc cagcccgaac cccgaaaaac aatacaggaa gacaaggcac acgccgccgg
     6421 caagccacgg gcgcaaaacg ggcatttcaa tgtcccaaaa ccgccgccac gcccccgcgc
     6481 ccaacgtccg tgccgtctga agccgtgccg caggcacttg agcaaacccc tgatacgccg
     6541 ccctgaccaa cacgggcagg ttaaaaaaca cattgccgta caacaacaga tacggcgtat
     6601 cctgccggcc gcgccacaac agcccgtccg ccccgaacag agccagcacg cccacgcccg
     6661 ccaccagcgt cggcatcaca aacggcagca tcagcaggcg cagcaccaaa gcccgccccg
     6721 ggaacgccag ccgcgccagc acccacgcga caggcacgcc caaaggcagc accagcacac
     6781 aggttgccgc cgcctgaaac accgtccacg ccaaacgttt gaacatatag gcatccgaca
     6841 gcaccgcgcg ccacgccaaa ccgtcatacg ccgccaccgc ccacaaaggc gcaacgacca
     6901 ttaccgccaa aaaagccgaa ggcagcaggg aaaaagcacc ccgtaccgcc caacgccgtc
     6961 catccatcgc cttccccact tgaaacactg atgttgcgat tgtacccaaa agccccacat
     7021 accgtatatt tcaatccgac tacgtaccgt atccgccttc ctcccgccgt ctgaaatata
     7081 gcggattaac aaaaatcagg acaaggcggc gggccgcagg cagtacaaat ggtacggaac
     7141 cggttcgccc ggtgcttcag cgccttaggg aaccgttccc tttgagccgg ggcggggcaa
     7201 cgccgtaccg gtttttgtta atccactata aatcgttcaa ataaacagga atataacttc
     7261 agacaaacaa cttaccgccc gatttgtgct atcgttttcg cacaacttaa aaaaacctga
     7321 caattttgta cttttattac agagaaaggc tttacaaatg gacggccgga cacagacgct
     7381 gtccgcgcaa accttgttgg gcatttcggc ggcggcaatc atcctcattc tgattttaat
     7441 cgtcaaattc cgcatccgcg cgctgctgac actggtcatc gccagcctgc tgacggcttt
     7501 ggcaaccggt ttgcccacag gcagcatcgt caacgacgta ctggtcaaaa acttcggcgg
     7561 cacgctcggc ggcgtggcgc ttctggtcgg tctgggcgca atgctcggac gtttggtaga
     7621 aacatccggc ggcgcacagt cgctggcgga cgcgctgatc cggatgttcg gcgaaaaacg
     7681 cgcaccgttc gcgccgggcg ttgcctcgct gattttcggc ttcccgattt tcttcgatgc
     7741 cggactaatc gtcatgctgc ccatcgtatt cgccaccgca cggcgcatga aacaggacgt
     7801 actgcccttc gcgcttgcct ccgtcggcgc atttatccgt catgcacgtc ttcctgccgc
     7861 cccatccggg cccgattgcc gcttccgaat tttacggcgc gaacatcggc caggttttga
     7921 ttttgggtct gccgaccgcc ttcatcacat ggtatttcag cggctatatg ctcggcaaag
     7981 tgttggggcg cgccatccat gttcccgttc ccgaactgct cagcggcggc acgcgagaca
     8041 gcgacccgcc gaaagaacct gccaaagcag gaacggtcgt cgccgtcatg ctgattccca
     8101 tgctgctgat tttcctgaat accggcgtat cggccctcat cagcgaaaaa ctcgtgagcg
     8161 cggacgaaac ctgggttcag acggcaaaaa tgatcggttc gacacctgtc gcccttctga
     8221 tttccgtatt ggccgcactg ttggtcttgg gacgcaaacg cggcgaaagc ggcagcacgt
     8281 tggaaaaaac cgtggacggc gcactcgccc ccgtctgttc cgtgattctg attaccggcg
     8341 cgggcggtat gttcggcggc gttttgcgcg cttccggcat cggcaaggca ctcgccgaca
     8401 gcatggcgga tttgggcatt cccgtccttt tgggctgctt ccttgccgcc ttggcactgc
     8461 gtatcgcgca aggttcggca accgtcgccc tgaccaccgc cgccgcgctg atggctcctg
     8521 ccgttgccgc cgccggcttt accgactggc agctcgcctg tatcgtattg gcaacggcgg
     8581 caggttcggt cggttgcagc cacttcaacg actccggctt ctggctggtc ggccgcctct
     8641 tggatatgga cgtaccgacc acgctgaaaa cctggacggc caaccaaacc ctcatcgcac
     8701 tcatcggctt tgccttgtcc gcactgctgt ttgccatcgt ctgacagacg gaaaggatag
     8761 taaatgacta cgcattttgt cgttacgggc gtatgcggct gcggcaagac caccgccgcg
     8821 ccgtccctgc agaaacacct cggtcaatgt ccctatgccg aaggcgacga gttccacacc
     8881 caagccaacc gcgacaagat gggcgcgggt attccgctga ccgatgaaga ccgctatccg
     8941 tggttgggca atctgcgcga ctggatgacg caacaggcgc aaaacggcgc ggaccacacc
     9001 atcgtaacct gttccgccct caaacgcggc taccgcgaca ttctgcgcgg agccgaaggc
     9061 aaagccgcct tcatccacct cagtccgccg caagacatca acctcgaacg catgatgtcg
     9121 cgcaaagggc actacatgaa agcagggatg ctcgattcgc aactggaaat cctcgaggaa
     9181 ctgggcgaag gcgaatacgg ggtcaaaatc gccaaccccg gcacgcccga agcggtcgaa
     9241 gccgatattc tgaactgggt tgcctcggaa aacctgcttt gaagcaatat gccgtctgaa
     9301 gcccggcaca ggatgggttt cagacggcat aaacatcggg aacagaatga attacattga
     9361 tttaaaatgg aaaatacccg gctatcgtct cattttcgtt ttaatcagcc ataaaaatgc
     9421 cgtccgaaac cctttcagac ggcatttctg tcaaacgccg gacgcactca atccaaactc
     9481 aacagcaggt tgcggaacgc gttcgggtct ttgataaacg tcatctcgcc cgcctgcgga
     9541 aaatgaaaat ccaacaggcg cgacacccaa aaacggatgc agccggcgcg ttgggcggtc
     9601 gggaaatacg ccttttcccc ggcactcaag gggcgcacgc cctcataacc gccgataaac
     9661 gcctttttca acgcctcatc caacttattg tccgccgtcc ttgcccaatc gttgaccgca
     9721 atcgccaagt catacataaa attgccccgg caggcataat agaaatcgat gaagcccgat
     9781 acctgaccgc cgtcaagcaa cacattgtct ttaaacaggt cggcgtggat gatgcccgaa
     9841 ggcagatggt tgccgagatt gtccttcagc gcatcgattt cggaacacag cagcgcggca
     9901 tcgtcttgcg acaggacggg cagcagccgg gcgcacgctt ccgtccacca cgcatcgtaa
     9961 cgcgggtttt ccatttccaa agggaaatcg gcggcggcaa ggtgcatttt cgccaacatc
    10021 gcaccggtat ggaaacactg ctcagccgtc ggcagcgcgg tatccgaacc tttcaggcag
    10081 gcaaccaggc aggccggctt gcccgccaaa acggaatcga gccggccgtc tttgcgcgca
    10141 accggcgcgg caaccgccac gcccttcata ctcaaatgcc ggttaagctc cagaaaaaac
    10201 gacagctctt cctgtttcaa cacttcaaac acggtcagca cataacgtcc cgaagtcgtc
    10261 gtcagaaaat aattgctgtt ggtaatcccc tgcgcaatgc cctgcaggga aacaaattcc
    10321 cccaaatcgt aaccgctcag gaagccgcgc atttcatcat cggaaacact ggtatagaca
    10381 gacatatcaa cttacttgct caaaaacggg caaacccgcc gtcagaacgc cggacggcag
    10441 gcaaacatat aattcacatc ggtcgaatcg cacagggcat aagtttgcga caacacatgg
    10501 taagtcatac ccttcgtatc cgccacatcc aagcccgcct gacggcacat ccgcgccagc
    10561 tcggcaggcg cgatgaattt tttccagtcg tgcgtgcctt tggggacgaa cttcaacaga
    10621 tattccgccg ccacaatcag atgcaggtac gatttcgggt ttttattgat ggtggaaaaa
    10681 aacaccatgc cgtccggctt gaccagcttg gcacaagcac gcacgatggc ggcgggatcg
    10741 gggacgtgtt ccatcatttc catgcacgtt accacatcga acgagtgcgg ttccgcctcg
    10801 gcaaggtctt ccacgcggat acattcgtat tcgatatcgg cgacattgtt caaagccgcg
    10861 tgcaggcggg cggtttccag cgactgctcc gccatgtcga tgcccttgac aaacgccgcg
    10921 ccgcgccgcg ccatgctttc cgcaaggatg ccgcctccgc agcccacgtc caaaacccgt
    10981 ttgccgcaca aatccgcgtg tccgtcgata taatcgagcc ggagcggatt gatgtcgtgc
    11041 aaggttttga actcgcccga cttgtcccac catttgtcgg caatccggct gaatttggcg
    11101 atttccccct catcgacatt atattttttg tcggacattt tccctcccat ctgacgaacc
    11161 gcccactcca aaacccaaga ttatactatg gaacacaatg ccgtctgaaa gccttttcgg
    11221 gcggcgcggc gcaaatgcct tacaaatcct tacactttac ggcataatgg cggctcgctt
    11281 tttctggcag aaagacaaaa tatgcccacc aaaacccctt cactgttcgg cggcgcgatg
    11341 attatcgccg gcacggtcat cggcgcaggt atgctcgcca acccgaccgc cacatccggc
    11401 gtatggttta ccggctcgct ggccgtgttg ctgtacacct ggttttctat gctttccagc
    11461 ggcctgatga ttttggaagt caacacccat tatccgcacg gcgcaagttt cgacacgatg
    11521 gtcaaagacc tgctcggacg cggctggaac atcatcaacg gcatcgccgt cgccttcgtt
    11581 ttatacctgc tcacctacgc ctacatcttc gtcggcggcg acctgaccgc caaaggcatc
    11641 ggcagcgcag taggcggcaa aatttcgctc accgtcggac aactcgtctt cttcggcatc
    11701 ctcgcctttt gcgtatgggc atccgcacgc ttggtcgacc gctttaccgg cgtcctcatc
    11761 ggcggcatgg tattaacctt tatttgggca accggcggcc tggttgccga tgccaaaccg
    11821 tccgtcctct tcgacaccca agcccccgcc ggcaccggct actggattta cgccgccacc
    11881 gccctgcccg tctgcctcgc ttccttcggc ttccacggca acgtttccag cctgctcaaa
    11941 tactttaaag gcgacgcgcc caaagtggcg aaatccatct gggcaggtac gttggttgcc
    12001 ttggtaattt acgtcctctg gcaaaccgcc atccaaggca acctgccgcg caacgagttc
    12061 gcccccgtga ttgccgccga agggcaagtc tccgtcctga ttgaaaccct gtccaaattc
    12121 gcccaaaccg gtaatatgga taaaatattg tccctatttt cctacatggc aatcgccacc
    12181 tcgtttttag gcgtaacctt aggcctgttc gactacatcg ccgacatctt caaatggaac
    12241 gacagcatgt ccggccgcac caaaaccgcc gcgctgacct tcctgccgcc cctgatttcc
    12301 tgcctgctct tccccaccgg cttcgttacc gccatcggct gcgtcggcct ggcggcaacc
    12361 gtctggacag gcatcatccc cgccatgctg ctctaccgtt cgcgcaaaaa attcggcgca
    12421 ggcaaaacct ataaagttta cggcggcttg tggctgatgg tttgggtctt ccttttcggc
    12481 atcgccaaca tcgccgcaca ggtattgagc caaatggaac tcgtccccgt atttaaagga
    12541 taaaggcaaa atgccgtttg aaacccgccg gcggcttcag gcggcattgc cgcaacaaac
    12601 ggcaaccgta ttccggcaca cagcgcatta ccctaccccc tcacgcacaa atcccgcccc
    12661 gacaggcgcg ggacgcaacc ataaaggaac aatgatgaag ctcaaaatag acattgcaac
    12721 caacaacttc aaacacggcg gcggcacgga acgctacaca ttggatttgg taaagggtct
    12781 gaacagacaa aacatcacac cggccgttta tgcgacgaaa tttgatcacg gcattcccga
    12841 atacgccatg atcgaacccc atcttgtcga tcaacgccgg acgctgaaaa aactacgctc
    12901 gttcctcttt tcaagccggc tcgctcaaac cagaaaaaac agtgccgcca aactgattgc
    12961 ctgccaccac gccgattacg ccgacctcct catctgcggc ggcacgcact tgggctacct
    13021 gcaccatatg gcgcaaaaac cgaacctgct cgaccgcctc gccatacgcc gcaaccgcag
    13081 caactacgcc accgccaaac tgattgtggc gcattcccat atg
//
LOCUS       DAWTQS010000059        12495 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.059, whole genome shotgun sequence.
ACCESSION   DAWTQS010000059 DAWTQS010000000
VERSION     DAWTQS010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12495
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.059"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            40..1184
                     /locus_tag="WNA25_001664"
                     /pseudo
     CDS             40..1184
                     /locus_tag="WNA25_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258633.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            1747..1929
                     /locus_tag="WNA25_001665"
     CDS             1747..1929
                     /locus_tag="WNA25_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824381.1"
                     /translation="MFWYVIGFCAFVVALLSLWVNAGAFGMQEDDTPQSDYEKRLGLG
                     AKLKNKNTPKAGEKRQ"
     gene            complement(1990..2991)
                     /locus_tag="WNA25_001666"
     CDS             complement(1990..2991)
                     /locus_tag="WNA25_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HFA9824382.1"
                     /translation="MIDGQTTEPKQKTRIIPAPMRGLVDDVMRDLLTRIGGYDECVSE
                     FVRITHTVHSRSIWLKYVPEIANGNKTFSGTPCTVQLLGSDADNMAANALEAVRFGAD
                     KIDLNFGCPAPTVNKHKGGAILLKEPELIFHIVKTLRGRLPAHIPLTGKMRLGYEDKS
                     PALECACAIAEGGACGLTVHARTKAEGYEPPAHWEWIRKIHDTVNIPVTANGDVFSLQ
                     DYIGIKTISGCNSVMLGRGAVIRPDLARQIKQYENGGPVKDTDFAEVSTWIGQFFELC
                     LTKEANNKYPIARLKQWLGMMKKTFDPAQTLFDRVRTVKDADEVRRILNAFEHEMDV"
     gene            complement(3068..6901)
                     /locus_tag="WNA25_001667"
     CDS             complement(3068..6901)
                     /locus_tag="WNA25_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/FMN-binding oxidoreductase"
                     /protein_id="HFA9824383.1"
                     /translation="MTTTTAPQRIREIPYNYTSYTDREIVIRLLGDEAWQILQDLRGQ
                     RKTGRSARMLFEVLGDIWVVVRNPYLVDDLLEHPKRRAALVREMRHRLNEIRKRRDDN
                     QQVDVLIAAAEKAVERFDGSFDETRQKRRQILERLSKITKPHNIMFDGLARVTHVTDA
                     TDWRVEYPFVVVNPDTEAEIAPLVRALIELDLVIIPRGGGTGYTGGAVPLDANSAVIN
                     TEKLDKHRGVEYVELAGLDGKHPIIRCGAGVVTRRVEETAHQAGLVFAVDPTSADASC
                     VGGNVAMNAGGKKAVLWGTALDNLAYWNMVNPQGEWLRIERVRHNFGKIHDEETAVFD
                     VHTLDSDGINIVKTERLEIPGHKFRKVGLGKDVTDKFLSGLPGVQKEGTDGIITGVAF
                     VLHKMPKYTRTVCMEFFGTVATATPSIVEIRDFLLAHDSVRLAGLEHLDWRYVRAVGY
                     ATKAAGKGRPKMVLLADVVSDDEAAVEAAAEHICELARARDGEGFIAVSPEARKTFWL
                     DRSRTAAIAKHTNAFKINEDVVIPLERLGEYSDGIERINIELSIQNKLKLCAALEQYL
                     SGKLPIDKMGTDLPTAELLGERGKHALAHVAAVKARWDWLLAHLDAPLADYKSRYGTA
                     VHAAPEAKDDESCFTAFRDFRLRVSVKADVMKPLAEIFSGKTDTKIIQGLGKIHAKTV
                     RSRVFVALHMHAGDGNVHTNIPVNSDDAEMLQTAYRSVERIMKIARSLGGVISGEHGI
                     GITKLEFLTDEDLQPFWNYKNQVDPKHTFNRHKLMKGSDLRNAYTPSFELLGAESLIM
                     EKSDLGTIADSVKDCLRCGKCKPVCSTHVPRANLLYSPRNKILGVGLLTEAFLYEEQT
                     RRGVSVKHFEELMDIGDHCTVCHRCVKPCPVNIDFGDVTVAVRNYLADSGHKRFAPAA
                     SMGMAFLNATGPKTIKALRAAMIQTGFPAQNFAYKIGKLLPIGTKKQKAEPKATVGKA
                     PIKEQVIHFINRPLPKSVPAKTPRSLLGIEDGKSIPIIHNPAAPEDAEAVFYFPGCGS
                     ERLFSQIGLAVQAMLWHVGVQTVLPPGYMCCGYPQDAGGNKAKAEEMSTNNRVAFHRM
                     ANTLNYLDIKTVVVSCGTCYDQLEKYRFEEIFPGCRIIDIHEYLLEKGVKLDGVKGQQ
                     YLYHDPCHTPIKTMNATQMASSLMGQKVVLSDRCCGESGMFAVKRPDIATQVKFRKQE
                     EIEKNLKELPQGEPVKMLTSCPACLQGLSRYSDDNNMPADYIVIEMAKHILGENWLDE
                     FVKKANNGGVEKVLL"
     gene            7162..7258
                     /gene="ffs"
                     /locus_tag="WNA25_001668"
     ncRNA           7162..7258
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="WNA25_001668"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            7481..7900
                     /locus_tag="WNA25_001669"
     CDS             7481..7900
                     /locus_tag="WNA25_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824384.1"
                     /translation="MKKLLMITLTGMLAACSTGVNVGRLMVEMPQGERSVVVQVPATN
                     NPLSDAVAVGMIKTSGSPSASNMIEMLGADNINVGVAGGSQMFNKATALYSLNHAKKV
                     GNNVSVYMTGDSESDKADLENAANAKNIKLHYFFNQK"
     gene            7997..9688
                     /gene="dld"
                     /locus_tag="WNA25_001670"
     CDS             7997..9688
                     /gene="dld"
                     /locus_tag="WNA25_001670"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFA9824385.1"
                     /translation="MNASQLISSLTQTVGEKYIITDPAKTEQYRQGYRFGEGKALAVV
                     RPGSILEMWKILQACVEADVIVITQAANTGLTGGSTPDGNDYDRDIVIVNTMRMNIIQ
                     TINNNEQVVCLPGSTLNQLELLLKPLGREPHSVIGSSCIGASVLGGVCNNSGGALVQR
                     GPAYTEMALFAQINEEGRLELVNHLGIDLGDMPEEILTNLQGHHYQKKDIKQDAGKGH
                     DHAYCEHVRQVDEPTAARFNADPARHYEASGCAGKLMVFAVRLDTFPQEKQTAVFYIG
                     TNDINELTDIRRAALGEFESLPVSGEYIHRHAFDIADVYGKDTFYVIKKFGTHQLPKL
                     FDLKARVDRFGKKVSFLPKHFSDKAMQFVSKFLPDHLPKSMRDYRDKYEHHLILKMGG
                     KGVDEARAFLKEYFSHHGGAFFECNAEETQAAMLHRFAVASAAIRYRSVHDDEVEDLV
                     ALDIALRRDDRDWFEKLPPEIDNKIIHKLYYGHFMCHVFHQDYIIKKGNGCMALEHEM
                     LHLLDQRGAQYPAEHNVGHLYEAKPALKQFYRKLDPTNSFNPGIGKTSKKKNWAE"
     gene            9873..10244
                     /locus_tag="WNA25_001671"
     CDS             9873..10244
                     /locus_tag="WNA25_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HFA9824386.1"
                     /translation="MKPYKIYTHPALPPQAVKQGWSWPGLLFGTLWACFKRMWGLGLG
                     MTGAMFVLAVFAQLVYGDTPATDSAFNVLGLAVSVWFGAKGNSLYARHLLSRGYTELP
                     ETVEAANPQAALAQYFGRGGG"
     gene            10384..10932
                     /locus_tag="WNA25_001672"
     CDS             10384..10932
                     /locus_tag="WNA25_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFA9824387.1"
                     /translation="MARLTVHTLETAPEAAKPRVEAVPKNNGFIPNLIGVLANAPEAL
                     AFYQEVGKLNAANSLTAGEVEVIRIIAARTNQCGFCVAGHTKLATLKKLLSEQSLNAA
                     RALAAGEFDDAKLGALAAFTQAVMAKKGAVSDDELKAFFDAGYNRQQAVEVVMGVALA
                     TLCNYANNLAQTEINPKLQAYA"
     gene            11070..12152
                     /locus_tag="WNA25_001673"
     CDS             11070..12152
                     /locus_tag="WNA25_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HFA9824388.1"
                     /translation="MNAQTLLANVAELVKTKLKPLVDDIDRKGYYPEAFMRELGAIGA
                     VGIEAEGGNGLGLATRIAVLREIGKECGATSFSAWCQAACAWYLHQTPNQAVKDKYLA
                     DILQGKVLAGTGMSNTVKHLAGIEKHNLQAECVDGGYKVNGALPWVSNIGEDHIWANT
                     AQIGGGYVMFITGGQREGVSLQNCPEFCALEGTRTFSLNFKDVFIPDEDIIAAPEQFA
                     GYIQSIKAGFILLQIGIGAGVIDGSLGIIRLANIVNAEVNSYLDDGYDSLKARLDGAW
                     AETERLAGLAWDNTPDNLATLKLREAAAVLALAAAQSAALHSGAKGYLMRSPAQRRVG
                     EAMFVAIVTPAIKHLCKEIAAIEAAK"
     gene            12285..12455
                     /locus_tag="WNA25_001674"
     CDS             12285..12455
                     /locus_tag="WNA25_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HFA9824389.1"
                     /translation="MAQYMCGPCGWIYDEELGDPEHGIAPGTKFEDIPDDWKCPECGV
                     GKEDFYLLDFVI"
BASE COUNT         2903 a         3383 c         3413 g         2796 t
ORIGIN      
        1 tgcccgatgt ttttagcaaa tttacaaaag gaagccgata tgcgaaaaaa acttaccgcc
       61 ctcgtattgt ccgcactgcc gtttgcggca gttgccgatg tcagcctgta cggcgaagtc
      121 aaagctggtg tggaaggcag gaacatccgg ctgcagttga ccgagccacc ctcagaaggt
      181 caaacgggca atacagttac taaggccaaa agccgcatca ggacgaaagt cagtgatttc
      241 ggctcgttta tcggctttaa gggggtgggg atttgggcgg cgggctgaag gctgtttggc
      301 agctcgagca agacgtatcc gttgccggcg gcggcgcgac ccgttggggt aacagggaat
      361 cctttatcgg cttggcaggc gaattcggca cggcgctcgc cggtcgcgtt gcgaatccgt
      421 ttggcgatgc cagcaaagcc attgatcctt gggacagcaa taataatgtg gcttcgcaat
      481 tgggtatttt caaacgccac gacggtatgc cggtttccgt gcgttacgat tcccccggat
      541 tttccggttt cagcggcagc attcaatttg ttccgagtca aaacagcaag tccgcctata
      601 cgcctgctac tttcacgctg gaaagtaatc agatgaaacc agttccggct gttgtcggca
      661 agccggggtc ggatgtgtat tatgccggtc tgaattacaa aaatggcggc tttttcggaa
      721 attatgccct taaatatgcg aaacacgcca atgaggggca tgatgctttc tttttgttct
      781 tgctcggcag agcgagtgat accgatccat tgaaaaacca tcaggtacac cgcctgacgg
      841 gcggctatgg ggaaggcggc ttgaatctcg ccttggcggc tcagttggat ttgtctgaaa
      901 atgccgacaa aaccaaaaac agtacgaccg aaattgccgc cactgcttcc taccgcttcg
      961 gtaatacagt cccgcgcatc agctatgccc atggtttcga ctttgtcgaa cgcagtcaga
     1021 aacgcgaaca taccagctat gatcaaatca tcgccggtgt cgattacgat ttttccaagc
     1081 gcacttccgc catcatgtct gccgcttggc tgaaacgaaa taccggcatc ggcaactaca
     1141 ctcaaattaa tgccgcctcc gttggtctgc gccacaaatt ctaaatatcg gggcggtgaa
     1201 gcggatagct ttgtttttga tggcttgcct tcattcttcg attgcaatct gactgccaat
     1261 ctgcttcagc cccaaacaaa aatccggata cggaagaaaa gcggcaataa agatagcaaa
     1321 tcccgtctga agccctgatt gggcttcaga cgggatttcg catacggctt cccttttcag
     1381 attcggatta aacatgctat atttatgaat ctttcaaacc gttttccaaa tgaggtaagg
     1441 aaatgagcga aaccgcgaaa atcaaactca acgaccggcc cgaaaatgca gaaaaaccaa
     1501 acgaaaaagt cgaactgccc attgtcgata acgacaaaaa aggcggacac ggcgaaggcg
     1561 gttgctgcgg ctgatacgtt tttcggcaaa gcagggcatc tgaaaagttt cgcaaaattt
     1621 tcaggtgccc ttttttgttt tcaaggtaaa tgaatgaaat actgcggtca gaagatttat
     1681 ttgctcgtgc gcttgtgata aattcggcac tgtatttgat atgattttaa caaatttgga
     1741 ggggcaatgt tttggtatgt aatcggcttt tgcgcctttg tcgtcgcgct gctgtcgctg
     1801 tgggtcaatg ccggcgcgtt cggtatgcag gaagacgata ccccgcaatc ggattatgaa
     1861 aaacgtttgg gtttgggggc gaaactgaaa aacaaaaata caccgaaagc aggggaaaaa
     1921 cggcaataaa gataacaaat gccgtctgaa aggttttcag acggcatttg ctttgaagcg
     1981 cgggagacat caaacatcca tctcatgctc aaatgcgttc aggattcggc gcacttcatc
     2041 cgcatcttta accgttcgga cgcggtcgaa cagcgtttgc gccgggtcga acgttttttt
     2101 catcatgccc agccattgct taagacgggc gatcgggtat ttgttgtttg cctcttttgt
     2161 caggcacagc tcgaaaaact gccctatcca tgtggaaact tcggcaaaat ccgtgtcttt
     2221 gaccggtccg ccgttctcgt attgcttgat ttggcgcgcc aaatcggggc ggatgaccgc
     2281 accgcgaccg agcatcacgc tgttgcagcc gctgattgtt ttgatgccga tatagtcctg
     2341 caggctgaaa acgtcgccgt tggcggtaac gggaatattg acggtatcgt ggattttcct
     2401 tatccattcc caatgcgccg ggggttcgta gccctcggct ttggtgcgcg cgtgcacggt
     2461 cagtccgcac gcgccccctt cggcaatcgc acaggcgcat tccaaagcag ggcttttgtc
     2521 ttcatagcct agccgcattt tccccgtcag cggaatatgt gcaggcaaac gtccgcgcag
     2581 cgttttgacg atgtggaata tcagttccgg ctctttcaga agaattgcgc cgcctttgtg
     2641 tttgttgacg gtgggcgcgg ggcagccgaa gttcaaatcg attttgtccg cgccgaatcg
     2701 gacggcttcc aatgcgtttg ccgccatatt gtccgcatcg ctgcccagaa gttggacggt
     2761 gcagggcgtg ccggaaaacg ttttgtttcc gttggcgatt tcggggacat attttaacca
     2821 tatggatcgt gaatgcacgg tatgggtaat gcgtacaaat tcgctgacgc attcgtcgta
     2881 gccgccgata cgtgtcagca ggtcgcgcat cacatcgtct accagccccc gcatgggggc
     2941 tggtatgatt ctggtttttt gtttgggttc ggttgtttgt ccgtcaatca tgattcgggt
     3001 aggtatttga ttcaaacaat gccgtctgaa ggctttcggc gttcagacgg catttccgtg
     3061 tccgttgtta tagcaacact ttctctacgc cgccgttgtt ggcttttttt acaaactcat
     3121 ccagccagtt ttcgccgagg atgtgtttcg ccatttcaat gacgatgtag tcggcaggca
     3181 tattgttgtc gtcggaatag cggctcagtc cttgcaggca ggcggggcag gaggtcagca
     3241 ttttgacggg ttcgccctgc ggcagctctt tgaggttttt ctcgatttcc tcttgtttgc
     3301 ggaacttgac ctgtgtggcg atgtcggggc gtttgacggc gaacataccg gattcgccgc
     3361 agcagcggtc gcttaaaacg actttctgcc ccatcaggct gctggccatt tgggtggcgt
     3421 tcatggtttt aatcggggtg tggcaggggt cgtggtagag gtattgctga cctttcacgc
     3481 cgtcgagttt cacgcctttt tcgagcaggt attcgtggat gtcgatgatg cggcagccgg
     3541 ggaagatttc ctcgaagcgg tatttttcaa gctggtcgta acaagtgccg caactgacga
     3601 cgacggtttt gatgtcgagg tagttgaggg tgttcgccat acggtggaag gcgacgcggt
     3661 tgttggtgct catttcttcg gcttttgcct tgttgccgcc agcgtcttgc ggatagccgc
     3721 aacacatata gccgggcggc aggacggttt gtacgccgac gtgccagagc atggcttgga
     3781 cggcgagtcc gatttggctg aacaaacgct ccgaaccgca gccggggaag tagaacacgg
     3841 cttcggcatc ttcgggcgcg gcggggttgt ggatgatggg gatgcttttg ccgtcttcga
     3901 tgcccaataa ggagcgcggt gttttggcgg gtacgctttt gggcagtggg cggttgatga
     3961 aatggataac ctgttcttta atcggggctt tgccgacggt tgccttgggt tcggcttttt
     4021 gctttttcgt gccgatcgga agaagcttgc cgattttgta ggcaaagttc tgcgcgggga
     4081 agccggtctg tatcatcgcg gcgcgcaggg ctttgatggt tttcggaccg gtggcgttca
     4141 aaaacgccat acccattgaa gccgcaggcg caaagcgttt gtggccggaa tcggcaaggt
     4201 agttgcggac ggctacggta acgtcgccga agtcgatgtt gacggggcag ggtttgacgc
     4261 agcggtggca cacggtgcag tggtcgccga tgtccatcag ttcttcaaaa tgtttgacgg
     4321 aaacgccgcg ccgggtttgt tcttcgtata agaaggcctc ggtcagcaaa cccacgccga
     4381 ggattttgtt gcgcgggctg tacagcaggt tggcgcgcgg aacgtgggtg gagcagacgg
     4441 gtttacattt gccgcagcgc aggcagtctt tgacggaatc ggcaatcgtg ccgaggtctg
     4501 atttttccat aatcagcgat tccgcgccca acagctcgaa agacggcgtg taggcgttgc
     4561 gtaagtccga acctttcatc agtttgtggc ggttgaaggt gtgcttgggg tcgacttggt
     4621 ttttgtagtt ccaaaacggt tgcaaatctt catcggtgag gaattcgagc ttggtgatgc
     4681 cgatgccgtg ttcgccggaa atcacgccgc cgagcgaacg ggcgattttc ataatgcgtt
     4741 ccacagaacg gtaggccgtc tgaagcattt cggcatcgtc tgagttgacc ggaatattgg
     4801 tgtggacgtt accgtcgccg gcatgcatat gcagggcgac aaagacgcgg ctgcgtacgg
     4861 ttttggcgtg gattttgccc aagccttgga taattttggt atcggttttg ccgctgaaga
     4921 tttcggcaag cggcttcatt acgtctgctt tgacagacac gcgcaggcgg aaatcgcgga
     4981 aggcggtgaa gcagctttca tcgtctttgg cttcgggcgc ggcgtggacg gctgtaccgt
     5041 agcgtgattt gtagtcggca agcggcgcat ccaaatgggc gagcagccag tcccaacgcg
     5101 ctttgacggc ggcaacgtgg gcgagggcgt gtttgccgcg ttcgcccaac agttcggcgg
     5161 tcggcaggtc ggtgcccatt ttgtcgatgg ggagtttgcc cgaaagatat tgctccaaag
     5221 cggcacagag tttgagtttg ttttggatgg aaagctcgat gttgatgcgt tcgatgccgt
     5281 ccgaatactc gcccagccgc tccagcggaa tcaccacgtc ttcgttgatt ttaaaggcgt
     5341 tggtgtgttt ggcgatggcg gcggtgcggc tgcggtcgag ccaaaaggtt ttgcgcgctt
     5401 cgggcgatac ggcgataaag ccttcgccgt cacgggcgcg ggcaagttcg cagatgtgtt
     5461 cggcggctgc ctctacggcg gcttcgtcgt ctgaaaccac gtccgccagc aagaccattt
     5521 tcggtcgtcc tttgcccgcc gctttggtgg cgtagccgac ggcgcggaca taacgccagt
     5581 ccaaatgctc caaacccgcc agccgcacgc tgtcgtgtgc gagcagaaaa tcgcggattt
     5641 cgacgataga aggcgtggcg gtggcgaccg tgccgaaaaa ctccatacac acggtgcgcg
     5701 tgtatttcgg cattttgtgc aatacgaagg caacgccggt aatgatgccg tccgtacctt
     5761 ctttctgcac gccgggcagt ccgctcaaaa atttgtcggt aacgtctttg cccaaaccga
     5821 ctttgcggaa tttgtgtccg gggatttcca agcgttcggt tttaacgatg ttgatgccgt
     5881 ctgaatccag cgtgtgcacg tcgaacacgg cagtttcttc gtcgtggatt ttgccgaaat
     5941 tgtggcgcac gcgttcgata cgcagccatt cgccttgagg gttaaccatg ttccagtagg
     6001 cgaggttgtc caacgccgtc ccccacagca cggctttttt gccgcccgcg ttcatcgcca
     6061 cattgccgcc cacgcaggac gcatcggcgg aagtcggatc gacggcgaac accaagcccg
     6121 cctgatgcgc ggtttcttcc acccgccgcg taaccacgcc cgcgccgcac cggataatcg
     6181 gatgtttgcc gtccaagcct gccagctcaa cgtattccac gccgcgatgc ttgtccagct
     6241 tttccgtatt gatgactgcg ctgtttgcgt ccaaaggtac tgcgccgccg gtataacccg
     6301 taccgccgcc gcgcgggata ataaccaagt ccaattcgat taaggcgcgc accaaagggg
     6361 caatctctgc ctcggtatca ggattgacga cgacaaacgg atactccacg cgccagtcgg
     6421 ttgcatctgt aacgtgcgtt acgcgagcca agccgtcaaa catgatgttg tgcggcttgg
     6481 tgattttgct taagcgttcc aaaatctgcc gccgcttttg gcgcgtttcg tcaaagctgc
     6541 cgtcaaaacg ctccactgcc ttttccgccg ccgcaatcaa cacatccact tgctgattgt
     6601 cgtcgcggcg tttgcggatt tcatttaagc gatggcgcat ttcgcgtacc aacgcggcgc
     6661 ggcgtttcgg atgctccagc aaatcatcga ccagatacgg attgcgcacg accacccaaa
     6721 tatcgcccaa cacttcaaac aacatccgcg ccgaacgtcc tgttttgcgt tgaccgcgca
     6781 ggtcttgcag gatttgccac gcttcatcgc ccaacaggcg gatgacgatt tcgcggtcgg
     6841 tataagaagt atagttgtag ggaatttccc gaatacgctg cggggcggta gtcgtggtca
     6901 tggcgtgtcc gtgtttggtt gtttttattt gagtgttgtc cgaacggtcg gttgcagaca
     6961 aattttgaaa ggcaataatg tagtttaatt cttaatattt ttcaatgccg aaacaggaaa
     7021 taccctcata gaaacaaaat gacgctttga aaaattgttg acaatattat cggatataag
     7081 ttttagacag tgtttccggc gtatgaccgt atcaaaacgg ttgagttttt cacaaaaccc
     7141 gttataatgc cgtttctccg aggcgggtct ccccgcatgg caaatcggaa caccgggtca
     7201 ggggcggaag ccagcagccc actccgatgc gccagtgccg ggggtttggt ccgccgccct
     7261 atttgaaacg ccggagcttc gatgttccgg cgttcccgta tcgcaccgat gccgtctgaa
     7321 agccgttcag acggcatttc ctttactttg ccataacgca tcattacaat gtatccatac
     7381 aggccaatga agaaccggct tcataaaaat ggaagtatgg ttcaatttca agttatatga
     7441 cgaacagggc ggtttgatta aagactaaag gagtaggcaa atgaaaaaac ttctaatgat
     7501 aaccctcacc ggtatgcttg cagcttgttc aacaggtgtc aatgtcggcc ggttgatggt
     7561 tgaaatgccg cagggagaac gctctgttgt cgtgcaggtt cccgcgacga ataacccgct
     7621 ttccgatgcg gtggctgtcg gaatgattaa aacatccgga tcgccttcgg catcaaatat
     7681 gattgaaatg ctcggcgcgg acaatatcaa cgtcggcgtg gcgggaggca gccaaatgtt
     7741 taataaggcg accgcacttt attccttaaa ccatgcaaag aaagtcggaa ataatgtcag
     7801 tgtctatatg acgggcgata gcgaaagcga caaggccgat ttggaaaacg cggcaaatgc
     7861 caaaaatatt aaattacatt atttctttaa ccaaaaataa tttgcagcgt gcaacttgcc
     7921 gcaccaaaat atccaaattg aacatacggc ggcgtaccgg gatttgcgcc gccgcttatc
     7981 ccgataggag agtgttatga acgcctcgca attaatcagc agcctgaccc aaaccgtagg
     8041 cgaaaaatac atcatcaccg accccgcgaa aaccgaacaa taccgccaag gctaccgttt
     8101 cggcgaaggc aaggcgttgg cggtggtgcg cccgggaagc attttggaaa tgtggaaaat
     8161 tctgcaggcc tgcgtcgaag cggacgtgat tgtgattacg caggcggcga ataccggact
     8221 gaccggcggc tcgacccccg acggcaacga ttacgaccgc gacatcgtga ttgtgaacac
     8281 catgcggatg aacatcatcc aaaccatcaa caacaacgaa caagtcgtct gcctgcccgg
     8341 ctcgaccctg aaccagcttg aactgctgct gaaacctttg ggacgagaac cgcattcggt
     8401 catcggctct tcttgcatcg gcgcgtccgt tttaggcggc gtgtgcaaca actccggcgg
     8461 cgcgttggta cagcgcggcc cggcctacac cgaaatggcg ttgttcgccc aaatcaacga
     8521 agagggcagg ttggaactgg tcaaccactt gggcatagac ttgggcgaca tgcccgaaga
     8581 aatcctgacc aacctgcaag gtcatcatta tcagaaaaaa gacatcaaac aagacgcggg
     8641 caaaggacat gaccacgcct attgcgaaca cgtccgccaa gtggacgaac cgaccgccgc
     8701 gcgtttcaat gccgaccccg cccgccatta cgaagcctcg ggctgcgcgg gcaagctgat
     8761 ggtttttgcc gtccgtttgg acaccttccc gcaagaaaaa caaaccgccg tgttctacat
     8821 cggcacgaac gacatcaacg agctgaccga catccgccgc gccgccttgg gcgaatttga
     8881 aagcctgccc gtttccggcg aatacatcca ccgccacgct ttcgacattg ccgacgtgta
     8941 cggcaaagac acgttctacg tcatcaaaaa attcggtacg caccaactgc cgaaattatt
     9001 tgacttgaag gcgcgcgtgg acaggttcgg caaaaaagtc agcttcctgc ccaaacattt
     9061 ttccgacaag gcaatgcagt tcgtcagcaa attcctgccc gaccacctgc ccaaatccat
     9121 gcgcgattac cgcgacaaat acgaacacca cctgattctg aaaatgggcg gaaaaggcgt
     9181 ggatgaggcg cgcgcgttct taaaagaata tttttcacac cacggcggcg cgtttttcga
     9241 gtgcaacgcc gaagaaaccc aagccgcgat gctgcaccgt ttcgccgtcg cttccgccgc
     9301 catccgctac cgttccgtgc acgacgacga agtggaggac ttggtcgcgc tggatatcgc
     9361 cctgcgccgc gacgaccgcg actggtttga aaaactgccg ccggaaatcg acaataaaat
     9421 catccataaa ttgtattacg ggcattttat gtgccacgtt ttccatcagg attacatcat
     9481 caaaaaaggc aacggctgta tggcgttgga acacgaaatg ctacatctct tagaccaacg
     9541 cggcgcgcaa tatcccgccg aacacaacgt cggccatttg tatgaagcca agcccgcgct
     9601 caaacagttt taccgcaaac tcgacccgac caacagcttc aacccgggta tcggcaaaac
     9661 cagcaaaaag aaaaactggg cggaataagc gcgtccgctt tgaaggcagg caatgccgtc
     9721 tgaaggcaaa acaccgttca gacggcattg tcggatctgt cttccccgac gggcatgttc
     9781 aagccgtatg tctgcacacg ggcgcgcgtc tcacaaataa agtctgatcc taccttccca
     9841 aagggcaggg tttcaaccga aaaggaaaca cgatgaaacc atacaaaatc tacacccatc
     9901 ccgccctgcc gccccaagcc gtcaaacaag gctggtcgtg gccgggtctt ttgttcggca
     9961 cgctgtgggc gtgtttcaaa agaatgtggg gtttggggct ggggatgacg ggcgcgatgt
    10021 tcgtgttggc ggtgtttgcc caactggttt acggcgacac gcccgccaca gactcggcgt
    10081 ttaacgtatt gggcttggcg gtttccgtct ggttcggcgc aaagggcaac agcctttatg
    10141 cacgccacct tttatcgcgc ggctataccg aattgcccga aacagtcgaa gccgccaacc
    10201 cccaagccgc attggcgcaa tatttcggac gcgggggcgg gtaggcgtgt ttcccggcaa
    10261 tgccgtctga aaggcttcag gcggcatttc ttataacttt ttgtttgtgg cacagtcttt
    10321 cctattccgt tccgaaatgc gtaccatgcg cccgtcccca atatttgcga acaaggaaag
    10381 aaaatggcac gtttaaccgt acacaccctc gaaaccgccc ccgaagccgc caaaccgcgc
    10441 gtagaggccg tacccaaaaa caacggcttt atccccaacc tcatcggcgt attggcaaac
    10501 gcccccgaag ccttggcgtt ttaccaagaa gtcggcaagc tcaacgccgc caacagcctg
    10561 accgccggcg aagtcgaagt catccggatc attgccgcac gcaccaacca atgcggcttc
    10621 tgcgtggcag ggcacaccaa actcgcaacc ctgaaaaaac tcctgtccga gcaatccctc
    10681 aatgccgccc gcgctttggc ggcaggtgaa tttgacgatg ccaaactcgg cgcgcttgcc
    10741 gccttcaccc aagccgtaat ggcgaaaaaa ggcgcagtat ccgacgacga actcaaagca
    10801 tttttcgatg cgggctacaa ccggcagcag gcagtcgaag tcgtaatggg cgtagccttg
    10861 gcaactttgt gcaactacgc caacaacctc gcccaaaccg aaatcaaccc caaattgcag
    10921 gcatacgcct aagcaaacaa aaccgcctga atgcaggcgg tttttcaaaa acaccccctc
    10981 aaacaaaaac aagccgccca agcggcggga aaagcagccg cgcatcaggc gtgcccccga
    11041 tttcccccca aaaaccttaa ggaacaaaga tgaacgccca aaccctgctt gccaacgttg
    11101 ccgaactcgt caaaaccaag ctcaaacccc tcgtcgacga catcgaccgc aaaggatact
    11161 accccgaagc atttatgcgc gaactcggcg caatcggcgc agtcggtata gaagccgaag
    11221 gcggcaacgg cttaggcttg gcaacacgaa tcgccgtctt gcgcgaaatc ggcaaagaat
    11281 gcggcgcgac ctccttcagc gcgtggtgtc aggcggcttg cgcgtggtat ctgcaccaaa
    11341 cgcccaacca agccgtcaaa gacaaatacc tcgccgacat cctgcaaggc aaagtattgg
    11401 cgggcaccgg aatgtccaat accgtcaaac accttgccgg cattgaaaaa cacaaccttc
    11461 aagccgagtg cgtggacggt ggttacaaag tcaacggcgc gctgccgtgg gtgtccaaca
    11521 tcggcgaaga ccacatttgg gcgaataccg cccaaatcgg cggcggctac gtcatgttca
    11581 tcacaggcgg gcaaagggaa ggcgtaagcc tgcaaaactg ccccgaattt tgcgccctcg
    11641 aaggcacgcg caccttcagc ctgaacttca aagacgtatt tatccccgac gaagacatca
    11701 tcgccgcgcc cgaacagttt gccggctaca tccaaagcat caaagcaggc tttatcctcc
    11761 tgcaaatcgg catcggcgcg ggcgtgatag acggcagcct cggcatcatc cgcctcgcca
    11821 acatcgtcaa tgcagaagtc aacagctatc tcgacgacgg ctacgacagc ctcaaagcaa
    11881 ggctggacgg cgcgtgggca gaaaccgaac ggctcgccgg attggcgtgg gacaacacgc
    11941 ccgacaacct cgccaccctc aagctgcgcg aagccgccgc cgtactggcc cttgccgctg
    12001 cgcaatccgc cgccctgcat tccggcgcga aaggctacct gatgcggagt cccgcccaaa
    12061 ggcgcgtcgg cgaagcgatg tttgtcgcca tcgtaacccc cgcaatcaaa cacctctgca
    12121 aagaaatagc agcaatcgaa gccgcaaaat aaacaggcgg aaaatgccgt ctgaagcaca
    12181 aaccgcccaa ggcgggacag atgcggcggc acgcttcaga cggcatccga accaaacccc
    12241 ggcggcccgc tgcaaaaaag cacggtacaa acaaggaaac acaaatggct caatatatgt
    12301 gcggcccctg cggctggatt tacgatgaag aactcggcga cccggaacac ggcatcgccc
    12361 ccgggacaaa gtttgaagac atccccgacg actggaaatg ccccgaatgc ggcgtgggca
    12421 aagaagattt ctacctgttg gatttcgtga tatagcggca gccgcacccc ttggaaaatc
    12481 cctcggcgtg ttccg
//
LOCUS       DAWTQS010000060        11983 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.060, whole genome shotgun sequence.
ACCESSION   DAWTQS010000060 DAWTQS010000000
VERSION     DAWTQS010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11983
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.060"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            1..486
                     /gene="def"
                     /locus_tag="WNA25_001675"
     CDS             1..486
                     /gene="def"
                     /locus_tag="WNA25_001675"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009118779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFA9824390.1"
                     /translation="MQYPDERLHTVAKPVEQVDERIRKLVADMFETMYESRGIGLAAT
                     QVDVHERVVVMDLTEDRSEPRVFINPVIVEKDGETTYEEGCLSVPGIYDAVTRAERVK
                     VEALNEKGEKFTLEADGLLAICVQHELDHLMGIVFVERLSQLKQGRIKTKLKKRQKHT
                     I"
     gene            574..1500
                     /locus_tag="WNA25_001676"
     CDS             574..1500
                     /locus_tag="WNA25_001676"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFA9824391.1"
                     /translation="MKVIFAGTPDFAAAALKAVAAAGFEIPLVLTQPDRPKGRGMQLT
                     APPVKQAALELGLRVAQPEKLRNNAEALQMLKEVEADVMVVAAYGLILPQDVLDTPKH
                     GCLNIHASLLPRWRGAAPIQRAIEAGDAETGVCIMQMDIGLDTGDVVSEHRYAIQPTD
                     TANEVHDALMEIGAAAVVADLQQLQSKGRLNAVKQPEEGVTYAQKLSKEEARIDWSES
                     ADIIERKIRAFNPVPAAWVEYQGKPMKIRRAEVVAQQGTAGEVLSCSADGLVVACGES
                     ALKITELQPAGGRRMNIAAFAAGRSIEAGAKL"
     gene            1579..2838
                     /gene="rsmB"
                     /locus_tag="WNA25_001677"
     CDS             1579..2838
                     /gene="rsmB"
                     /locus_tag="WNA25_001677"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFA9824392.1"
                     /translation="MSMALAQKLAADSIAAVAEGRNLQDVLAHIRAAHPQLTAQENGA
                     LQDIAYGCQRYLGSLKHMLAQMLKKPIDNPQLESLLLAALYQLHYTRNAPHAVVNEAV
                     ESIAKIGRGQYRSFANAVLRRFLREREKLAASCKKDDVAKYNLPLWWVAYLKNHYPKH
                     WHNIAAALQSHPPMTLRVNRRHGNAESYLEKLAVEGIAAKALDEYAVTLEEAVPVNRL
                     PGFAEGLVSVQDFGAQQAAYLLNPKDGERILDACAAPGGKTGHILELADCRVTALDID
                     AGRLKRVEDNIARLGFQTASAACADARDLAAWYDGKPFDAILADVPCTASGVARRNPD
                     VKWLRRPTDALKTARRQEALLDALWQALKQGGRMLLATCSVFVEENDGQLQKFLNRHA
                     DAEPIESRVLLPNKHQDGFYYALIQKH"
     gene            2804..3403
                     /locus_tag="WNA25_001678"
     CDS             2804..3403
                     /locus_tag="WNA25_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HFA9824393.1"
                     /translation="MAFITRLFKSIKQWLVLLPMLSVLPDAAAEGIAATRAEARITDG
                     GRLSISSRFQTELPDQLQQALRRGVPLNFTLSWQLSAPTIASYRFKLGQLIGDDDNID
                     YKLSFHPLTNRYRVTVGAFSTDYDTLDAALRATGAVANWKVLNKGALSGAEAGETKAE
                     IRLTLSTSKLPKPFQINALTSQNWHLDSGWKPLNIIGNK"
     gene            3403..5523
                     /locus_tag="WNA25_001679"
     CDS             3403..5523
                     /locus_tag="WNA25_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HFA9824394.1"
                     /translation="MRRFLPIAAICAVVLLYGLTAATGSTSSLADYFWWIVSFSAMLL
                     LVLSAVLARYVILLLKDRRNGVFGSQIAKRLSGMFTLVAVLPGLFLFGISAQFINGTI
                     NSWFGNDTHEALERSLNLSKSALDLAADNAVSNAVPVQIDLIGTASLSGNMGSVLEHY
                     AGSGFAQLALYNAASGKIEKSINPHQFDQPLPDKEHWEQIQQTGSVRSLESIGGVLYA
                     QGWLSAGTHNGRDYALFFRQPIPENVAQDAVLIEKARAKYAELSYSKKGLQTFFLVTL
                     LIASLLSIFLALVMALYFARRFVEPILSLAEGAKAVAQGDFSQTRPVLRNDEFGRLTK
                     LFNHMTEQLSIAKEADERNRRREEAARHYLECVLDGLTTGVVVFDEKGRLKTFNKAAE
                     QILGMPLAPLWGSSRHGWHGVSAQQSLLAEVFAAIGAAAGTDKPVQVEYAAPDDAKIL
                     LGKATVLPEDNGNGVVMVIDDITVLIRAQKEAAWGEVAKRLAHEIRNPLTPIQLSAER
                     LAWKLGGKLDDQDAQILTRSTDTIIKQVAALKEMVEAFRNYARAPSLKLENQDLNALI
                     GDVLALYEAGPCRFEAELAGEPLMMAADTTAMRQVLHNIFKNAAEAAEEADMPEVRVK
                     SETGQDGRIVLTVCDNGKGFGKEMLHNAFEPYVTDKPAGTGLGLPVVKKIIGEHGGRI
                     SLSNQDAGGACVRIILPKTVETYA"
     gene            5516..6793
                     /locus_tag="WNA25_001680"
     CDS             5516..6793
                     /locus_tag="WNA25_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HFA9824395.1"
                     /translation="MRSSDILIVDDEVGIRDLLSEILQDEGYSVALAENAEEARKLRH
                     QARPAMVLLDIWMPDCDGITLLKEWAKNGQLNMPVVVMSGHASIDTAVEATKIGAIDF
                     LEKPISLQKLLSAVENALKHGAAQIETGSVFDKLGNSAAIQEMNREVEAAAKRTSPVF
                     LTGEAGSPFETVARYFHKNGTPWVSPARVEYLIDMPMELLQKAEGGVLYVGDIAQYSR
                     NIQTGITFIIGKAERCRVRVIASCSYAAGSDGISCEEKLAGLFSESVVRIPPLSMQHE
                     DIPFLIQGIACNVAESQKITPTAFSDDALAVLSRYDWPGNFEQLQNVVTTLLLEADGQ
                     EIGIGAASSALGRGVPADGTGHMACGFDFNLPLRELREEVERRYFEYHIAQEGQNMSK
                     VAQKVGLERTHLYRKLKQLGIGVSRRAGEKTEE"
     gene            6884..8071
                     /gene="dprA"
                     /locus_tag="WNA25_001681"
     CDS             6884..8071
                     /gene="dprA"
                     /locus_tag="WNA25_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFA9824396.1"
                     /translation="MTEDERFAWLQLAFTPYIGAESFLLLMRSFGSAQNALSAPAEQV
                     APAVRHKHALEAWRNAEKRALARQAAEAALEWEMRDGCRLMLLQDEDFPEMLTQGLTA
                     PPVLFLRGNVRLLHKPSAAIVGSRHATPQAMRIAKDFGRALGGKGIPTVSGMASGIDT
                     AAHQGALEAEGGTIAVWGTGIDRIYPPANKNLAYEIAEKGLIVSEFPIGTRPYAGNFP
                     RRNRLIAALSQVTLVVEAALESGSLITAGLAAEMGREVMAVPGSIDNPHSKGCHKLIK
                     DGAKLTECLDDILNECPGLLQNTGASSYSINKDTPDTGRRAVQTAYAPPPAAKMPSEG
                     AAGGTAVGGILDKMGFDPIHPDVLAGQLAMPAADLYAALLELELDGSVAAMPGGRYQR
                     IRT"
     gene            8097..8558
                     /locus_tag="WNA25_001682"
     CDS             8097..8558
                     /locus_tag="WNA25_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HFA9824397.1"
                     /translation="MTEVIAYLIEHFQDFDTCPPPEDLGMLLEEAGFDTMEIGNTLMM
                     MEVLLNSSEFSAEPAGSGALRVYSKEETDNLPQEVMGLMQYLIEEKAVSCEQREIIIH
                     ALMHIPGDEITVDTAKVLTLLLLWANKSELPVLVGDELMSALLLDNKPTMN"
     gene            8636..10942
                     /gene="topA"
                     /locus_tag="WNA25_001683"
     CDS             8636..10942
                     /gene="topA"
                     /locus_tag="WNA25_001683"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFA9824398.1"
                     /translation="MAKNLLIVESPSKAKTLKKYLGGDFEILASYGHVRDLVPKSGAV
                     DPDNGFAMKYQLISRNGKHVDAIVAAAKEAENIYLATDPDREGEAISWHLLEILKSKR
                     GLKNIKPQRVVFHEITKNAVLDAVAHPREIEMDLVDAQQARRALDYLVGFNLSPLLWK
                     KIRRGLSAGRVQSPALRLICERENEIRAFEAQEYWTVHLDSHKGRSKFTAKLAQYNGA
                     KLEQFDLPNEAAQADVLKELEGKEAVVTAIEKKKRSRNPAAPFTTSTMQQDAVRKLGF
                     TTDRTMRTAQQLYEGIDVGQGAIGLITYMRTDSVNLADEALTEIRHYIENKIGKEYLP
                     SAAKQYKTKSKNAQEAHEAIRPTSVYRTPESVKPFLSADQFKLYQMIWQRTVACQMTP
                     AKFDQTTVDITVGKGVFRVTGQVQTFAGFLSVYEESSDDEESEDGKKLPEMSEGDKLP
                     VDKLYGEQHFTTPPPRYNEATLVKALEEYGIGRPSTYASIISTLKDREYVTLEQKRFM
                     PTDTGDIVNKFLTEHFAQYVDYHFTAKLEDQLDEIADGKRRWIPVMDKFWKPFIKQVE
                     EKEGIERAKFTTQELNETCPKCGEHKLQIKFGKMGRFVACAGYPECSYTRNVNETAEE
                     AAERIAKAEAEQAELDGRECPKCGGRLAYKYSRTGSKFIGCANYPKCKHVEPLEKPKD
                     TGVQCPQCKKGNLVERKSRYGKLFYSCSTYPDCNYATWNPPVAEECPNCHWPVLTIKT
                     TKRRGVEKVCPQKECGWKEQIEPPAPQE"
     gene            11190..11825
                     /locus_tag="WNA25_001684"
                     /pseudo
     CDS             11190..11825
                     /locus_tag="WNA25_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT         2937 a         3310 c         3395 g         2341 t
ORIGIN      
        1 ttgcaatatc ccgacgagcg tctgcacacg gtggcaaagc ctgtcgaaca agttgacgag
       61 cgcatccgga agctggttgc cgatatgttt gaaacgatgt acgaatcgcg cggcatcggg
      121 ctggcggcga cgcaggtcga tgtgcacgaa cgcgtggtcg tgatggattt gaccgaagac
      181 cgcagcgaac cgcgcgtgtt catcaacccc gtcatcgttg aaaaagacgg cgaaaccact
      241 tacgaagagg gctgcctgtc cgtaccgggc atttacgacg ccgttacccg cgccgaacgc
      301 gtcaaggtcg aggctttgaa cgaaaaaggc gaaaaattca cgctggaggc ggacgggctg
      361 ctggcgattt gcgtgcagca cgagttagat cacttgatgg gcatcgtgtt tgtcgaacgc
      421 ctttcccaac tcaagcaggg gcggattaag accaaactga aaaaacgtca gaaacatacg
      481 atttgattcg cttcgtcatg ccgtctgaac gctgcaaggc tttcagacgg cacagccttg
      541 tccgacaact ttacgcacac gcaggaacac gctatgaaag tcatctttgc cggcacgccc
      601 gattttgccg ccgccgcctt aaaagccgtt gccgccgccg gttttgaaat cccgctggtg
      661 ctgacccagc ccgaccgtcc gaaagggcgc ggtatgcaac tgaccgcccc gcctgtcaaa
      721 caagccgcgc tggaactcgg tttgcgcgtg gcacagcccg aaaaactgcg caacaacgcc
      781 gaagccctgc aaatgctcaa agaggtcgag gcggacgtaa tggtggttgc cgcgtacggc
      841 ttgattctgc cgcaggacgt gttggatacg ccgaaacacg gctgcctcaa tatccatgct
      901 tcgctgctgc cccgttggcg cggcgcggcg ccgattcagc gcgcgattga ggccggcgat
      961 gccgaaaccg gcgtgtgcat tatgcagatg gacatcggtt tggacaccgg cgatgtggtc
     1021 agcgaacacc gctacgccat ccaacctacc gatactgcca acgaagtcca cgacgcgctg
     1081 atggaaatcg gtgcggcggc ggttgttgcc gatttgcaac agcttcaaag caaaggccgt
     1141 ctgaacgcgg tcaaacagcc cgaagaaggc gtaacttacg cccaaaaact aagcaaagaa
     1201 gaagcgcgta tcgattggag cgaaagcgcg gacattatcg aacgcaaaat ccgcgccttc
     1261 aaccccgtgc ctgccgcgtg ggtcgaatat cagggcaagc cgatgaaaat ccggcgtgcc
     1321 gaagtggtgg cgcaacaagg tacggcaggc gaagtgttgt cctgttcggc ggacggtttg
     1381 gtcgttgcct gcggcgaaag cgcgctgaag attaccgaat tgcagcctgc cggcggcagg
     1441 cgtatgaata ttgcggcgtt tgcggcggga cggtctattg aagcaggggc gaagctgtaa
     1501 atcccttcag acggcattcc gatccgcaaa cgggaatgcc gtctgaaacc atcagttgaa
     1561 gaaagcgaat cacataatat gagtatggca cttgcccaaa aacttgccgc cgacagcatt
     1621 gcggcggttg ccgaagggcg caaccttcag gacgtgttgg cgcacatccg cgcggcgcat
     1681 ccgcagctga cggcgcagga aaacggcgcg ttgcaggata tcgcctacgg ctgccagcgt
     1741 tatttgggca gtttgaaaca tatgctcgcg cagatgctga aaaagcccat cgacaatccg
     1801 cagctcgaaa gcctgctttt ggcggcgttg taccaactgc attacacgcg caacgcgccc
     1861 cacgccgtgg tcaacgaagc tgttgaaagc atagccaaaa tcgggcgcgg gcagtaccgt
     1921 tcgtttgcca acgcggtttt gcgccgcttt ttgcgcgaac gtgaaaaatt ggcggcttcc
     1981 tgcaaaaaag acgatgtggc gaaatacaac ctgccgctgt ggtgggttgc ctacctgaaa
     2041 aaccattatc cgaaacactg gcacaacatc gccgccgcgc tgcaatccca tccgccgatg
     2101 acgttgcgcg tcaaccgccg gcacggcaac gccgaaagct atttggaaaa actggctgtg
     2161 gaaggtatcg cggctaaggc gttggacgaa tatgcggtta cgttggaaga agccgtgccg
     2221 gtaaaccgtc tgcccggttt tgccgaagga ctggtgtcgg tacaggactt cggcgcgcag
     2281 caggcggcgt atctccttaa cccgaaagac ggcgaacgga ttttggacgc gtgcgccgcg
     2341 ccgggcggca agacggggca tattttggaa ctggcggact gccgcgttac cgccttggac
     2401 atcgatgcag gccgtctgaa gcgtgtggag gacaatatcg cccgtttggg ctttcagacg
     2461 gcatcggcgg cgtgtgccga tgcgcgggat ttggcggcgt ggtatgatgg aaagcctttc
     2521 gacgcaatcc ttgccgacgt gccgtgcacc gcttccggcg tggcgcggcg caatcccgac
     2581 gtgaaatggc tgcgccgacc gaccgacgcg ctcaaaaccg cccgccggca ggaagccctg
     2641 ctggacgcac tttggcaggc gttgaaacag ggcggcagaa tgctgcttgc cacctgttcc
     2701 gtgttcgtcg aagaaaacga cgggcaactg caaaaattcc tcaaccgcca tgccgatgcc
     2761 gaaccgatcg aatcgcgggt gcttttaccg aacaaacacc aagatggctt ttattacgcg
     2821 cttattcaaa agcattaaac aatggcttgt gctgttgccg atgctctccg ttttgccgga
     2881 cgcggcggcg gagggcattg ccgcgacccg cgccgaagcg aggataaccg acggcgggcg
     2941 gctttccatc agcagccgct tccaaaccga gctgcccgac cagctccaac aggcgttgcg
     3001 ccggggcgta ccgctcaact ttaccttaag ctggcagctt tccgccccga caatcgcttc
     3061 ttatcggttt aaattggggc aactgattgg cgatgacgac aatattgact acaaactgag
     3121 tttccatccg ctgaccaacc gctaccgcgt taccgtcggc gcattttcca ccgattacga
     3181 cactttggat gcggcattgc gcgcgaccgg cgcggttgcc aactggaaag tcctgaacaa
     3241 aggcgcgttg tccggtgcgg aagcagggga aaccaaggcg gaaatccgcc tgacgctgtc
     3301 cacttcaaaa ctgcccaagc ctttccaaat caacgcattg acttctcaaa actggcattt
     3361 ggattcgggt tggaaacctc taaacatcat cgggaacaaa taatgcgccg cttcctaccg
     3421 atcgcagcca tatgcgccgt cgtcctgctg tacggattga cggcggcaac cggcagcacc
     3481 agttcgctgg cggattattt ctggtggata gtctcgttca gcgcaatgct gctgctggtg
     3541 ttgtccgccg ttttggcacg ttatgtcata ttgctgttga aagacaggcg caacggcgtg
     3601 ttcggttcgc agattgccaa acgcctttcc gggatgttca cgctggtcgc cgtactgccc
     3661 ggcttgttcc tgttcggcat ttccgcgcag tttatcaacg gcacgattaa ttcgtggttc
     3721 ggcaacgaca cccacgaagc cctcgaacgc agccttaatt tgagcaagtc cgcactggat
     3781 ttggcggcag acaatgccgt cagcaacgcc gttcccgtac agatagacct catcggcacc
     3841 gcctccctgt cgggcaatat gggcagtgtg ctggaacact acgccggcag cggttttgcc
     3901 cagcttgccc tgtacaatgc cgcaagcggg aaaatcgaaa aaagcatcaa tccgcaccaa
     3961 ttcgaccagc cgcttcccga caaagaacat tgggaacaga ttcagcagac cggttcggtt
     4021 cggagtttgg aaagcatagg cggcgtattg tacgcgcagg gatggttgtc ggcaggtacg
     4081 cacaacgggc gcgattacgc gctgttcttc cgccagccga ttcccgaaaa tgtggcacag
     4141 gatgccgttc tgattgaaaa ggcgcgggcg aaatatgccg aattgagtta cagcaaaaaa
     4201 ggtttgcaga ccttttttct ggtaaccctg ctgattgcct cgctgctgtc gatttttctt
     4261 gcgctggtaa tggcactgta ttttgcccgc cgtttcgtcg aacccattct gtcgcttgcc
     4321 gagggcgcaa aggcggtggc gcagggtgat ttcagccaga cgcgccccgt attgcgcaac
     4381 gacgagttcg gacgtttgac caagctgttc aaccatatga ccgagcagct ttccatcgcc
     4441 aaagaagcag acgaacgcaa ccgccggcgc gaggaagccg cccgccacta cctcgagtgc
     4501 gtgttggatg ggttgactac cggtgtggtg gtgtttgacg aaaaaggccg tttgaaaacc
     4561 ttcaacaagg cggcggaaca gattttgggg atgccgctcg cccccctgtg gggcagcagc
     4621 cggcacggtt ggcacggcgt ttcggcacag cagtccctgc ttgccgaagt gtttgccgcc
     4681 atcggtgcgg cggcaggtac ggacaaaccg gtccaggtgg aatatgccgc gccggacgat
     4741 gccaaaatcc tgctgggcaa ggcgacggta ttgcccgaag acaacggcaa cggcgtggtg
     4801 atggtgattg acgacatcac cgtgctgata cgcgcgcaaa aagaagccgc gtggggtgaa
     4861 gtggcgaagc ggctggcaca cgaaatccgc aatccgctca cgcccatcca gctttccgcc
     4921 gaacggctgg cgtggaaatt gggcgggaag ctggacgatc aggacgcgca aatcctgacg
     4981 cgttcgaccg acaccatcat caaacaggtg gcggcgttaa aagaaatggt cgaggcattc
     5041 cgcaattacg cgcgcgcccc ttcgctcaaa ctggaaaatc aggatttgaa cgccttaatc
     5101 ggcgatgttt tggccctgta cgaagccggc ccgtgccggt ttgaggcgga acttgccggc
     5161 gaaccgctga tgatggcggc ggatacgacc gccatgcggc aggtgctgca caatattttc
     5221 aaaaatgccg ccgaagcggc ggaagaagcc gatatgcccg aagtcagggt aaaatcggaa
     5281 acggggcagg acggacggat tgtcctgacg gtttgcgaca acggcaaggg attcggcaag
     5341 gaaatgctgc acaatgcttt cgagccgtat gtgacggata agccggcggg aacgggactg
     5401 ggtctgcctg tagtgaaaaa aatcattgga gaacacggcg gccgcatcag cctgagcaat
     5461 caggatgcgg gtggggcgtg tgtcagaatc atcttgccaa aaacggtaga aacttatgcg
     5521 tagcagcgat attttaattg tagacgacga agtcggcatc cgcgacctgc tgtcggaaat
     5581 cctgcaggac gaaggctatt cggtcgcgtt ggcggaaaat gccgaagagg cgcgcaagct
     5641 gcgccatcag gcgcgcccgg cgatggtgct gctggatatt tggatgcccg attgcgacgg
     5701 tatcaccctt ttgaaggagt gggcgaaaaa cgggcagctc aatatgccgg tggtggtgat
     5761 gagcgggcat gccagcatcg ataccgccgt agaagcgacc aaaatcggcg cgatcgattt
     5821 tttggaaaaa ccgatttccc tgcaaaagct gctgtctgcg gtcgaaaacg cgttgaaaca
     5881 cggtgcggcg caaatcgaaa cgggatcggt gttcgacaaa ctgggcaaca gtgccgcgat
     5941 tcaagagatg aaccgcgaag tcgaagcggc ggcaaagcgc acgtcccctg tatttttgac
     6001 gggcgaggcg ggttcgccgt ttgaaaccgt ggcgcgctat ttccataaaa acggtacgcc
     6061 gtgggtcagt ccggcaaggg tcgaatatct gatcgatatg ccgatggaac tgttgcagaa
     6121 ggcggagggc ggcgttttgt atgtcggcga catcgcccaa tacagccgca atatccaaac
     6181 cggcattacc tttatcatcg gcaaggcaga acgctgccgt gtcagggtga ttgcttcgtg
     6241 cagctacgcg gcaggttcag acggcatttc atgtgaagaa aaattggccg ggcttttttc
     6301 ggaatcggtc gtccgtattc cgcccctgag tatgcagcat gaagacattc ccttcctgat
     6361 acaggggatt gcctgcaatg tggcggaaag ccaaaaaatc acgcctaccg cattcagtga
     6421 cgatgcgctt gccgtattgt cccgttacga ctggccgggg aatttcgagc agttgcaaaa
     6481 cgtcgtgaca acgctgctgc tggaggcgga cgggcaggaa atcggtatcg gcgcggcttc
     6541 ttcggcactg gggcgcggtg tgcctgccga cggaacgggg catatggcgt gcgggttcga
     6601 ctttaacctg ccgctgcgcg aattaaggga ggaggtggag cggcgttatt tcgagtacca
     6661 catcgcccaa gaaggtcaga atatgagcaa agtggcgcag aaggtcggtt tggaacgcac
     6721 gcacctttac cgcaaactca aacagctcgg catcggcgtt tcgcgccggg cgggggaaaa
     6781 aaccgaagaa taggcccgga cggccggttt cccggctgcg ggcttttgtt ttcagacggc
     6841 atttggagaa aatgccgtct ggaatcgtaa ggggatggat tttatgacag aggacgaacg
     6901 tttcgcgtgg ctgcaattgg cgtttacgcc ctatatcggc gcggaaagtt tcctgctgct
     6961 gatgcgcagt ttcggcagcg cgcaaaatgc cttgtccgca ccggcggaac aggttgcccc
     7021 ggcggtgcgg cataagcacg cgcttgaggc ttggcgcaat gcggaaaaac gcgctttggc
     7081 gcggcaggcg gcagaagcgg cgttggaatg ggaaatgcgg gacggatgcc gcctgatgct
     7141 gcttcaggat gaagatttcc ccgaaatgct gacgcagggg ctgaccgcgc cgccggtttt
     7201 gtttttgcgc ggcaacgtgc ggctgctgca caagccttcc gccgccatcg tcggcagccg
     7261 ccatgccacg ccgcaggcga tgcggattgc caaagatttc ggcagggcgt tgggtgggaa
     7321 aggcattcct accgtgtcgg gtatggcttc gggcatcgac accgccgccc atcagggcgc
     7381 actggaggca gaaggcggca ccatcgccgt gtgggggacg ggcatagacc gcatttatcc
     7441 gccggccaac aaaaaccttg cctatgaaat cgccgaaaaa gggctgattg tcagcgagtt
     7501 ccccatcggc acgcggccgt atgccggcaa ctttccgcgc cgcaaccgcc tgattgccgc
     7561 gctgtcgcag gttacgctgg tggtcgaggc cgccttggaa tccggttcgc tgattaccgc
     7621 cggcctggcg gcagaaatgg ggcgcgaagt gatggcggta cccggttcga tagacaaccc
     7681 ccacagcaaa ggctgccaca aactgattaa agacggagcg aaactgacgg aatgtttgga
     7741 cgacatcctg aacgaatgcc cggggctatt gcaaaatacg ggtgcttcgt catattctat
     7801 aaataaggat acgcccgaca cgggccgccg cgccgttcag acggcatacg ccccgccgcc
     7861 tgccgcaaaa atgccgtctg aaggggcggc aggcggcaca gccgtcggcg gcatactgga
     7921 taaaatgggt ttcgacccca tccatcccga cgtgcttgcc ggacagttgg ctatgcctgc
     7981 cgcagatttg tatgccgcac tgttggaatt ggaattggac ggcagcgttg ccgccatgcc
     8041 cggcggcaga taccagcgta tccgaacttg aacgcacttt atattaagga acacgaatga
     8101 ccgaagtcat cgcctacctc atcgaacatt tccaagattt cgatacctgc ccgccgcccg
     8161 aagacttggg tatgctgctt gaagaagcgg gtttcgatac gatggaaatc ggcaacaccc
     8221 tgatgatgat ggaagtattg ctcaacagct ccgaattttc cgccgaaccc gccggcagcg
     8281 gcgcattgcg cgtgtacagc aaagaagaaa ccgacaacct gccgcaggaa gtgatggggc
     8341 tgatgcagta tctgattgaa gaaaaagccg tcagctgcga acagcgggaa atcatcatcc
     8401 acgcgctcat gcacatcccc ggcgacgaaa ttaccgtaga taccgccaaa gtgctgaccc
     8461 tgctgctttt atgggcaaac aagagcgagc tgcccgtatt ggtcggcgac gagctgatga
     8521 gcgcgctttt actcgacaac aaacccacga tgaactgaag cggcttcaga cggcgccggc
     8581 ccgcccgatg ccgtctgaaa caccggcgtc aaaaccacca tccagagaac gacaaatggc
     8641 gaaaaacctc ttaatcgtcg aatccccgtc caaagccaaa accctgaaaa aatatttggg
     8701 cggcgatttt gaaatcctcg cgtcctacgg acacgtccgc gacctcgtcc ccaaaagcgg
     8761 cgcggtcgat cccgacaacg gctttgcgat gaaataccaa ctcatcagcc gcaacggcaa
     8821 acatgtcgat gcgattgttg ccgctgccaa agaagccgaa aacatctacc tcgcaaccga
     8881 cccggacagg gaaggcgaag ccatttcctg gcatcttttg gaaatcctca aatccaaacg
     8941 cggcctgaaa aacatcaagc cgcagcgtgt cgtgttccac gaaatcacca aaaacgccgt
     9001 gctcgatgcg gttgcccatc ctcgcgaaat cgaaatggat ttggtcgatg cgcaacaagc
     9061 ccgtcgcgct ctggactatc tggtcggttt caacctctcg ccgttgctgt ggaaaaaaat
     9121 ccgacgcggt ttgagcgcgg gccgcgtgca aagccctgct ttgcgcctga tttgcgaacg
     9181 cgaaaacgaa atccgcgcgt ttgaagcgca ggaatattgg acggtacatc tagacagcca
     9241 caaaggccgc agcaagttca ccgccaaact cgcccaatac aacggcgcga aactcgaaca
     9301 attcgacctg ccgaacgaag ccgctcaagc cgatgtgttg aaagaactcg aaggcaaaga
     9361 ggccgtcgtt accgccatcg aaaagaaaaa gcgcagccgc aaccccgccg cgccgttcac
     9421 cacatccacc atgcagcagg atgccgtgcg taaacttggt ttcaccaccg accgcaccat
     9481 gcgtaccgcc cagcagcttt acgaaggtat agacgtaggg cagggcgcca tcggtctgat
     9541 tacctatatg cgtaccgaca gcgtgaattt ggctgacgaa gcgttaaccg aaatccgcca
     9601 ttacattgaa aacaaaatcg gcaaagaata tctgccgagt gccgccaaac aatacaaaac
     9661 caaatccaaa aacgcccaag aagcccatga agcgatccgc ccgacttccg tgtaccgcac
     9721 gcccgaaagc gtcaaaccct tcctgagtgc agaccagttt aaactttatc aaatgatttg
     9781 gcagcgcacc gtcgcctgtc agatgacgcc cgccaaattc gaccaaacca ccgtcgatat
     9841 taccgtcggc aaaggcgtgt tccgagtaac cggacaagtg caaaccttcg caggcttcct
     9901 cagcgtttac gaagaaagca gcgacgatga agagagcgaa gacggcaaaa aactgcccga
     9961 aatgagcgaa ggcgacaaat tgccggtcga caaactctac ggcgaacaac acttcaccac
    10021 tccgccgccg cgctacaacg aagccacact ggttaaagcc cttgaagaat acggcatcgg
    10081 ccgcccctcg acctacgcca gcatcatctc cacgctcaaa gaccgcgaat acgttaccct
    10141 tgagcaaaaa cgcttcatgc ccaccgacac aggcgacatc gtcaataaat tcctgaccga
    10201 acacttcgcc caatacgtcg attaccactt caccgccaaa ctcgaagacc agcttgacga
    10261 aatcgccgac ggcaaacgcc gctggattcc cgtgatggac aaattctgga aaccgttcat
    10321 caaacaagtg gaagaaaaag agggcatcga acgcgccaaa tttaccacgc aggaacttaa
    10381 cgaaacctgc ccgaaatgcg gcgaacacaa actgcaaatc aagttcggca aaatgggccg
    10441 cttcgtcgcc tgtgccggct atcccgaatg cagctacacg cgcaacgtca acgaaaccgc
    10501 cgaagaagcc gccgagcgca tcgccaaagc cgaggctgaa caagccgaac tcgacggacg
    10561 cgaatgcccc aaatgcggcg gtcggttagc gtataaatac agccgtaccg gcagcaaatt
    10621 catcggctgc gccaactacc ccaaatgcaa acacgtcgag ccgctggaaa aaccgaaaga
    10681 caccggcgtc caatgcccgc aatgcaaaaa aggtaacctc gtcgagcgca aatcccgcta
    10741 cggcaaactg ttttacagtt gcagcaccta tcccgactgc aactacgcca cttggaaccc
    10801 gcccgttgcc gaagaatgcc cgaactgcca ttggccggtc ttgaccatca aaaccactaa
    10861 acgccggggt gtggaaaaag tctgcccgca aaaagaatgc ggctggaaag aacaaatcga
    10921 accgcctgca ccgcaagagt gaagcggttg ggattaagat gaaagaaaat gccgtctgaa
    10981 aggggtttca gacggcattt gtaaattaga aggggctgtc ccagatggct ggggaaattt
    11041 aaattaagtc agaattatcc ctatgagaaa agccgtccaa gccggtacaa acaaaagaaa
    11101 cccatcggac tgtttgccgc aggggtaact gcaagaacgg cagcagggtt ggcagcagtt
    11161 aatcaaaaca cggcagcctg ttattttcta tggcgaaata gtgtttggca catcatcggg
    11221 aaaacttcct ttacacgcat ttggacgaaa tctgcgaaat tatggaggca tacgacatat
    11281 cgtttacgat tacttatccg tcaaaacagt ccgcatttgg aaatgtatga cggcgaagca
    11341 ggggcgggtg aaagttattt ccgtaaaggc agacgcggcc gcagtgccgc cggcaaagcc
    11401 gccgtattcg ggcttttgaa gcgcaacggc aaggtttgta cggttactgt cggacactcg
    11461 aaccgctatt ttattgccta ttatccgcgg acgggtgaaa cccggcagca ttgtttatac
    11521 ggattgttcg tagttacgat gtatcagatt gtaagcggat ttggccgttt ccgtatcaat
    11581 cgcggcacac attttgccga acggcaaaac catatcaacg caattgggaa cttttggaat
    11641 cgggcaaacg tcatttgcgc aagtttgacg gcattcccaa agagcatttc gggctgtatt
    11701 tgaaggagtg cggacggcgt tttgacaaca gtggggtaaa agttcgaatt tccattttaa
    11761 aacaattggt aaagcaggat ttatcccggt tggccgggat tttaaaaaat aaatacattc
    11821 tttaacaaaa caaatcatat cccggcaaaa caaatctgat aaaatacttg gcgtttatta
    11881 acaatctttt taataattta atcttagata tttggagttg atatgcatac ggtcgacaaa
    11941 aaacttctct tctcttctct tctcttctct tctcttctct tct
//
LOCUS       DAWTQS010000061        11735 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.061, whole genome shotgun sequence.
ACCESSION   DAWTQS010000061 DAWTQS010000000
VERSION     DAWTQS010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11735
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.061"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(241..1320)
                     /gene="apbC"
                     /locus_tag="WNA25_001685"
     CDS             complement(241..1320)
                     /gene="apbC"
                     /locus_tag="WNA25_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFA9824399.1"
                     /translation="MNIQNIRTLLDTVAVPNTARTLGGEKAVRSVGQRSDGIHIALHF
                     GFPVAHIAAALADAVQETLMPETGGAHIHLSIDTEIGTHKVRPGVATIKGVKNIIAVA
                     SGKGGVGKSTTTANLAAAMARMGARVGVLDADLYGPSQPTMLGVHDRKPDQKNQKLIP
                     VESSDGIQVMSIGFLVDTGQAVVWRGPMVSQALQQLMFQSEWDEVDYLFIDLPPGTGD
                     IQLTLSQRIPVTGSVIVTTPQDIALIDARKAVDMFRKVNIPILGVLENMSVHICSNCG
                     HSEALFGTDGGKDLAARLNVPLLGQLPLSLPVREAMDGGTPARLFDEHPAIARIYTDA
                     AFQIALGIADKGKDFSSRFPKIVVE"
     gene            1539..1614
                     /locus_tag="WNA25_001686"
     tRNA            1539..1614
                     /locus_tag="WNA25_001686"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1572..1574,aa:Lys,seq:ttt)
     gene            1687..2196
                     /locus_tag="WNA25_001687"
     CDS             1687..2196
                     /locus_tag="WNA25_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HFA9824400.1"
                     /translation="MKKKLLSGIKFAVRTALVFLLVSLFLDWVRKPDEPAGAAGRPLT
                     LLSGQRLTLGQFSRDRTVLVYFWGSWCGVCRYQSPIIDDLAADGVPVVGVAVRSGSAS
                     EVAAYMAKRGLGFPTVSDEDGGLARSWRIAATPAVVLVKNGKMVRYTTGISSYWGLRA
                     RIFQADFFG"
     gene            2537..2977
                     /gene="nadR"
                     /locus_tag="WNA25_001688"
     CDS             2537..2977
                     /gene="nadR"
                     /locus_tag="WNA25_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family adhesin repressor NadR"
                     /protein_id="HFA9824401.1"
                     /translation="MPTQSKHASINIGLIQAREALMTQFRPILNQANITDQQWRIIRL
                     LAENGTLDFQDLANQACILRPSLTGILTRLEKAGLVVRLKPSNDQRRVYLKLTSEGEK
                     LYEEIGEEVDERYDAIEEVLGREKMLLLKDLLAELAKIEDALNS"
     gene            3092..3592
                     /gene="hpaC"
                     /locus_tag="WNA25_001689"
     CDS             3092..3592
                     /gene="hpaC"
                     /locus_tag="WNA25_001689"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     reductase component"
                     /protein_id="HFA9824402.1"
                     /translation="MADLQKTFQTSFRDAMASCAAGVHVITTDGAAGRYGITMTAVAP
                     VTDEPPTVMLCINRSARIIPILSENGSLCINMLADEHQDVAEHFAGLTGLSPEERFAY
                     HIWHRGKTGQLEIEGALAHLHGHIVGKHEIGTHFVFYVRLDEIKNCGCKRPALLYFRR
                     QFRPLD"
     gene            3608..4303
                     /locus_tag="WNA25_001690"
     CDS             3608..4303
                     /locus_tag="WNA25_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HFA9824403.1"
                     /translation="MKAMILAAGRGERMRPLTDTTPKPLLDVAGKPLIGWHLCRLKQA
                     GFTEIVINHAWLGRQIENALGDGSAYGVNIAYSPEPAGGLETAGGIAQALPLLGGQPF
                     LVANGDVLTDIDFTAAFQTASSLPGHISAHLWLVGNPPHNPDGDFSLLPDGSVRPEVS
                     GGNGLTFSGVGIYRPEMFDGIEAGSVAKLAPVLLNEMRQNRVSGQKHTGLWLDVGTVC
                     RLKEAQALAAAWK"
     gene            complement(4357..4767)
                     /locus_tag="WNA25_001691"
     CDS             complement(4357..4767)
                     /locus_tag="WNA25_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFA9824404.1"
                     /translation="MGSNAWLFWALASAGFASLTAVFAKMGLQGIDSDFAAFIRTLVI
                     LAALVLFLTYTGKWQGVNGFTGRNRTFLVLSGLATGASWLAYFKALQLGNAPQVAPVD
                     KFSLVLVALMAVIFLDERPNTQEWIDLGLVTAAC"
     gene            4942..6618
                     /locus_tag="WNA25_001692"
     CDS             4942..6618
                     /locus_tag="WNA25_001692"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFA9824405.1"
                     /translation="MSFKTDAETAQSSTMRPIGEIAAKLGLNVDNIEPYGHYKAKINP
                     AEAFKLPQKQGRLILVTAINPTPAGEGKTTVTIGLADALRHIGKDSVIALREPSLGPV
                     FGVKGGAAGGGYAQVLPMEDINLHFTGDFHAIGAANNLLAAMLDNHIYQGNELNIDPK
                     RVLWRRVVDMNDRQLRNIIDGMGKPVDGVMRPDGFDITVASEVMAVFCLAKDISDLKE
                     RFGNILVAYAKDGSPVYAKDLKAHGAMAALLKDAIKPNLVQTIEGTPAFVHGGPFANI
                     AHGCNSVTATRLAKHLADYAVTEAGFGADLGAEKFCDIKCRLAGLKPDAAVVVATVRA
                     LKYNGGVERANLGEENLEALAKGLPNLLKHISNLKNVFGLPVVVALNRFVSDSDAELA
                     MIEKACAEHGVEVSLTEVWGKGGAGGADLARKVVNAIDNQPNNFGFAYDVELGIKDKI
                     RAIAQKVYGAEDVDFSAEASAEIASLEKLGLDKMPICMAKTQYSLSDNAKLLGCPEGF
                     RITVRGITVSAGAGFIVALCGNMMKMPGLPKVPAAEKIDVDEHGVIHGLF"
     gene            6732..7823
                     /gene="ychF"
                     /locus_tag="WNA25_001693"
     CDS             6732..7823
                     /gene="ychF"
                     /locus_tag="WNA25_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFA9824406.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTQSGIEAANYPFCTIEPNVGIV
                     EVPDPRMAELAKIVNPQKMQPAIVEFVDIAGLVAGASKGEGLGNRFLANIRETDAIVN
                     VVRCFDDDNIVHVSGKVDPIADIETIGTELALADLASVEKAIVREEKRARSGDKDAQK
                     LVDLCKKLLPHLDEGKPVRSFGLDAEERALLKPLFLLTAKPAMYVGNVAEDGFENNPH
                     LDRLKELAAKENAPVVAVCAAMESEIAELEDGEKAEFLAEMGLEEPGLNRLIRAGYDL
                     LGLQTYFTAGVKEVRAWTIHKGDTAPQAAGVIHTDFERGFIRAQVIAYDDFVSLGGEA
                     KAKEAGKMRVEGKEYVVQDGDVMHFLFNV"
     gene            7861..8100
                     /locus_tag="WNA25_001694"
     CDS             7861..8100
                     /locus_tag="WNA25_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824407.1"
                     /translation="MPSEAGFGGFRRSHTAGMQPHQNKIPPAFPICPPRFLQNKPPAL
                     PLREAVRYSEYPEPRYKMTFQTAFAQPAAFQVKTL"
     gene            8097..9972
                     /locus_tag="WNA25_001695"
                     /pseudo
     CDS             8097..9972
                     /locus_tag="WNA25_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259993.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
     gene            10034..11329
                     /gene="tyrS"
                     /locus_tag="WNA25_001696"
     CDS             10034..11329
                     /gene="tyrS"
                     /locus_tag="WNA25_001696"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFA9824408.1"
                     /translation="MSVIQDLQSRGLIAQTTDIEALDALLNEQKIALYCGFDPTADSL
                     HIGHLLPVLALRRFQQAGHTPIALVGGATGMIGDPSFKAAERSLNSAETVAGWVGSIR
                     SQLTPFLSFEGGNAAIMANNADWFGSMNCLDFLRDIGKHFSVNAMLNKESVKQRIDRD
                     GAGISFTEFAYSLLQGYDFAELNKRHGAVLEIGGSDQWGNITAGIDLTRRLNQKQVFG
                     LTLPLVTKSDGTKFGKTEGGAVWLNAKKTSPYQFYQFWLKVADADVYKFLKYFTFLSI
                     EEIGVVEAKDKASGSKPEAQRILAEEMTRLIHGEEALAAAQRISESLFAEDQSRLTES
                     DFEQLALDGLPAFEVSDGINAVEALVKTGLAASNKEARGFVNAKAVLLNGKPAEANNP
                     NHAAERPDDAYLLIGEYKRFGKYTILRRGKRNHALLVWK"
BASE COUNT         2601 a         3210 c         3268 g         2656 t
ORIGIN      
        1 tttcaaaccg cctgcaaccg aaaaatctgc cgcaatccgc tccctccccc gtgggggagg
       61 gccggggaga gggcattctc caagttgcgg caacctttcc caacaactca accgcctaaa
      121 tacaagcctt gcggcttgtt gccctctctc cagccctctc ccacgggaga gaggactatg
      181 aagcccgtcg gcaggcgcgg ggcttgggac ggcattgctg ttgcggtttc gggcgcggct
      241 ttattcgacg acaattttag ggaaccggct gctgaagtct ttgcctttgt cggcaatgcc
      301 cagggcgatt tggaatgcgg catcggtgta gattcgggcg atggcggggt gttcgtcgaa
      361 cagtcgcgcc ggtgtgccgc cgtccatggc ttcgcgcacg ggcaggctta ggggaagctg
      421 tccgagcagg gggacgttga ggcgggccgc caaatctttg ccgccgtccg tgccgaacag
      481 tgcttcgcta tgcccgcagt tggagcagat gtggacggac atgttttcca atacgcctaa
      541 aatgggaatg ttgaccttgc ggaacatatc cacagccttg cgcgcgtcta tcagcgcgat
      601 gtcctgcggc gtggttacga tgacggaccc ggttacgggg atgcgttggg acagcgtgag
      661 ttggatgtcg cccgtgccgg ggggcaggtc gatgaaaaga tagtccactt cgtcccactc
      721 gctttggaac atcagctgct gcaaggcttg gctgaccatc ggcccgcgcc agacgacggc
      781 ttggccggta tcgactagaa agccgatgga cataacctgt atgccgtctg aagattcgac
      841 gggaatcagt ttttggtttt tctgatcggg tttgcggtcg tgtacgccca gcatcgtggg
      901 ctggctcgga ccgtaaaggt cggcatcgag cacgccgacg cgtgcgccca tgcgcgccat
      961 tgcggcggca aggttggcag tggtggttga tttgcccacg ccgccttttc ccgatgcgac
     1021 ggcaatgatg tttttcacgc ctttgatggt ggcgacgccg ggccggactt tgtgtgtgcc
     1081 gatttcggtg tctatggaca gatggatgtg cgcgccgcct gtttcgggca tcagggtttc
     1141 ttgtacggca tcggcgaggg cggcggcgat gtgcgcgaca gggaagccga aatgcagggc
     1201 gatgtggatg ccgtctgaac gctgtccgac cgaacggacg gccttttcgc cgccgagcgt
     1261 gcgtgccgta ttcggaacgg cgacggtgtc gaggagggtg cggatgtttt gtatattcat
     1321 gatgtgtgcc tctgttcgca agtcgtgtga ggggaagata gacggaaagt gtacatgatt
     1381 ttgggcagtt tgcttttttc ttgtgcgaac ttttacacgg tatgcggcgg attgtgaaaa
     1441 tccgttgcca aaatatcccg tgcaaacagc agtctgcaat taagcctgta aaatttgctt
     1501 gacggcgggg aacggggctg tataatgcac ggcttatcgg gtcgttagct cagccggtag
     1561 agcagcggac ttttaatccg ttggtcgagc gttcgaatcg ctcacgaccc accagataac
     1621 cggaagccaa gtttcgagac ttggcttttt tgtttgcctg ccggggcgta ccgtttgcgg
     1681 gggtgtatga aaaagaaact gctttcgggc atcaaatttg ccgttcggac ggcattggtg
     1741 tttttgctgg tgtcgctgtt tttggattgg gtacgcaagc ccgacgaacc tgccggggcg
     1801 gcagggcggc ctttgaccct gctgtcgggg cagcggctga ctttgggaca gtttagccgg
     1861 gataggacgg tgctggtgta tttttgggga agctggtgcg gcgtgtgccg ttatcagtcg
     1921 ccgataatcg atgatttggc ggcggacggc gtgccggtcg tcggcgtggc ggtacgttcc
     1981 ggcagcgcgt ccgaagtggc ggcatatatg gcaaaacggg gcttgggctt tccgactgtc
     2041 agcgatgagg acgggggttt ggcaaggtct tggcggattg ccgcaacgcc tgccgtcgtt
     2101 ttggtcaaaa atgggaaaat ggtccgctat acgacgggaa tcagcagtta ttggggcttg
     2161 cgcgcacgaa tttttcaggc ggattttttc ggttaacttt tgtttttgtc aaactttccg
     2221 ctatgtagag aatcaaacgg catatgcctt gcgcggcgga tgcggcggct tttgtttttt
     2281 gggaaagagc cggcgcgcct gccgatgtaa tcccttgttg tgattgtggg aaaaatagat
     2341 taaaatataa ctattaaaat attttcagat aggattatcg gaattaaagt cttttatacc
     2401 cggtcgtccg atgaggttta tagcgtattg ttgctatatg ttcgttttgt tatataacgg
     2461 ttgcatcaaa aattacgccc acaggctttc ccgacggttt gaaagtttga ttttcgataa
     2521 cttggagact taaacaatgc ctacccaatc aaaacatgcg tctatcaata tcggtctgat
     2581 acaggcaagg gaagccctga tgacccaatt caggcctatt ctgaatcagg cgaatattac
     2641 cgatcagcaa tggcggatta tccgtctttt ggcggaaaac ggcacattgg attttcagga
     2701 tttggcgaat caggcgtgca ttttgcgccc cagcctgacc ggtatcctga cccgccttga
     2761 aaaagcgggg ttggtcgtcc gcctgaaacc ttccaacgac caacggcgcg tttatctgaa
     2821 gctgacttcc gagggcgaga agctgtatga ggaaatcggc gaagaagtgg acgagcgtta
     2881 cgacgctatc gaggaagtgc tgggccgcga gaaaatgctg ctgcttaaag acctgttggc
     2941 agaacttgcc aaaatcgagg atgcgttgaa ctcgtaatac gccgtaatgc gcggaaacgt
     3001 ccgaccgacg gctttttgaa tcagaactgc tgcacatggg ggatgccttg tgtgcagcat
     3061 tcttatatag gggacggttt aaaggggaaa aatggcggat ttgcagaaaa cttttcaaac
     3121 ttcgttccgt gatgcgatgg catcctgtgc ggcaggcgtt catgtcatca cgacagacgg
     3181 tgcggcaggg cgttacggca ttacaatgac ggcggttgcg ccggttaccg acgagccgcc
     3241 gaccgtgatg ctgtgcataa accggagtgc gcgaatcatt ccgatcctgt cggaaaacgg
     3301 cagcctctgc atcaatatgc tggcggacga acatcaggat gttgccgaac attttgccgg
     3361 gctgaccggc ctgtcgcccg aagagcggtt tgcctaccat atttggcatc gcggcaaaac
     3421 gggacaactt gaaatagaag gcgcgttggc gcacctgcac gggcatattg tcggcaaaca
     3481 tgaaatcggc acgcattttg tgttttacgt caggctcgac gaaatcaaaa actgcgggtg
     3541 caaacgcccc gcgctgctgt atttcagacg gcagtttagg cctttagact gatattcgga
     3601 cagatatatg aaagcgatga tactggcggc aggacgcggc gagcgtatgc gccctttgac
     3661 cgacaccact ccgaagccgc tgctcgatgt ggcgggtaag cctctaatcg gttggcactt
     3721 gtgccgtctg aagcaggcgg ggtttaccga aatcgtcatc aaccacgctt ggctgggtcg
     3781 gcagatagaa aatgctttgg gcgacggctc ggcttatggc gtgaacatcg cctattcgcc
     3841 cgaacccgca ggcggtttgg aaacggcagg cggcatcgcg caggcattgc cgctgttggg
     3901 tgggcagccg tttttggtgg ccaacggcga cgtgctgacc gacatcgatt ttaccgccgc
     3961 gtttcagacg gcatcgtccc tgcccgggca catttccgca catttgtggc tggtgggaaa
     4021 tcccccgcac aaccccgacg gcgatttttc cctgctgccc gacggcagcg tgcggccgga
     4081 agtatccggc ggcaacggac tgacattcag cggcgtgggt atttaccgtc ctgaaatgtt
     4141 tgacggaatc gaagcgggca gtgtggcaaa actcgcgccc gtattgctga acgaaatgcg
     4201 gcaaaaccgc gtgagcggtc agaagcatac gggcctgtgg ctggatgtcg gcacggtatg
     4261 ccgtctgaag gaagcgcaag cccttgcagc ggcttggaag tgaaaactcg gtttcaggcg
     4321 gtatggcgga ttcggtttaa cgtttcagtg ccaacgtcaa cacgccgccg taaccagccc
     4381 caagtctatc cattcctgcg tgttcgggcg ttcgtctaag aaaatcaccg ccatcagcgc
     4441 gaccaagacc aggctgaatt tgtcgacggg cgcgacttgc ggggcgttgc ccagttgcag
     4501 ggctttgaag taggcgagcc aagatgcgcc ggtggcgagt ccggacaaaa ccagaaacgt
     4561 ccggttgcgc cccgtgaagc cgtttacgcc ctgccatttg ccggtgtagg tcaaaaacaa
     4621 taccaaggcg gcgaggatga ccaaggtgcg gataaaggcg gcgaaatccg aatctatgcc
     4681 ctgcaagccc attttggcga aaacggcggt caatgaggcg aagcctgccg atgccaatgc
     4741 ccaaaacagc catgcgttgc tgcccatgtt ttctcctttg attgtgaaca atatgaacgg
     4801 tatttttgtt gctgcgtcaa aaatttcact gcgggtttgg tgcggataac gttataatat
     4861 gcctgatatt attttcaatc cacctgtttg tcgcctgatg ctttcagacg gcatgtccct
     4921 cctcatttct aaaggaaaat catgagcttc aaaaccgatg ccgaaaccgc ccaatcctcc
     4981 accatgcgcc cgattggcga aattgccgcc aagctgggtt tgaacgttga caacattgag
     5041 ccttacggtc attacaaagc caaaatcaat cctgccgaag cgttcaagct gccgcaaaaa
     5101 caaggcaggc tgattttggt taccgccatc aacccgactc cggcgggcga aggcaaaacc
     5161 accgtaacca tcggtttggc ggacgcattg cgccatatcg gcaaagactc tgtgattgct
     5221 ttgcgcgagc cttctttggg tccggtgttc ggcgtgaaag gcggcgcggc aggcggcggc
     5281 tacgcgcaag ttttgccgat ggaagacatc aacctgcact tcaccggcga cttccacgcc
     5341 atcggtgcgg cgaataacct cctcgccgcc atgctcgaca accatatcta ccaaggtaac
     5401 gagttgaaca tcgaccccaa acgcgtgctg tggcggcgcg tggtcgatat gaacgaccgc
     5461 cagttgcgca acatcatcga cggtatgggc aagcctgttg acggcgtgat gcgtcccgac
     5521 ggcttcgaca tcaccgtcgc ctccgaagtg atggcggtat tctgccttgc caaagacatc
     5581 agcgatttga aagagcgttt tggcaatatt ctcgtcgcct acgccaaaga cggcagcccc
     5641 gtttacgcca aagatttgaa ggcacacggc gcgatggcgg cattgctaaa agatgcgatt
     5701 aagcccaatt tggtgcaaac catcgaaggc actccggcct ttgtacacgg cggcccgttc
     5761 gccaacatcg cccacggctg caactccgtt accgcaaccc gtctggcgaa acaccttgcc
     5821 gattacgccg taaccgaagc aggcttcggc gcggacttgg gtgcggaaaa attctgcgac
     5881 atcaaatgcc gccttgccgg tttgaaacct gatgcggcag tcgtcgtggc gactgtccgc
     5941 gccctgaaat acaacggcgg cgtggaacgc gccaaccttg gtgaagaaaa cctcgaagcc
     6001 ttggcaaaag gtttgcccaa cctgttgaaa cacatttcca acctgaaaaa cgtattcgga
     6061 ctgcccgtcg tcgttgcgct caaccgcttc gtgtccgact ccgatgccga gttggcgatg
     6121 attgaaaaag cctgtgccga acacggcgtt gaagtttccc tgaccgaagt gtggggcaaa
     6181 ggcggcgcgg gcggcgcgga tttggcgcgc aaagtcgtca atgccatcga caaccaacct
     6241 aataacttcg gtttcgccta cgatgtcgag ttgggcatca aagacaaaat ccgtgcgatt
     6301 gcccaaaaag tgtacggcgc ggaagatgtc gatttcagcg cggaagcgtc tgccgaaatc
     6361 gcctcgctgg aaaaactggg cttggacaaa atgccgatct gcatggcgaa aacccaatat
     6421 tcattgagcg acaacgccaa actcttgggc tgccccgaag gcttccgcat caccgtacgc
     6481 ggtatcactg tttccgccgg cgcgggcttc atcgttgcgt tgtgcggcaa tatgatgaaa
     6541 atgccgggcc tgccgaaagt tccggctgcc gagaaaatcg atgtggacga acacggcgtg
     6601 attcacggct tgttctgaac ggtttttgaa accggatgcc gtctgaagcc gtttcagacg
     6661 gcattttttc ggaacgcggg cggcggtatg ctataatccg ccgttaaatt tctctatttt
     6721 caggaaaaac catgagtttg aaatgcggca tcgtcggttt gcccaacgtc ggcaaatcca
     6781 ccctttttaa cgcgctgacc caatcgggca tcgaagcggc aaactatccc ttctgcacca
     6841 tcgaacccaa cgtcggcatc gtcgaagtgc ccgacccgcg tatggcggag ctggcgaaaa
     6901 tcgtcaatcc gcaaaaaatg cagcccgcca tcgtcgagtt tgtcgatatt gccggcttgg
     6961 ttgcgggcgc gagcaaaggc gaaggcttgg gcaaccggtt ccttgccaac atccgtgaaa
     7021 ccgatgccat cgtcaacgtc gtgcgctgct ttgacgacga caacatcgtc cacgtttccg
     7081 gcaaagtcga tccgattgcc gacatcgaaa ccatcggcac cgaattggcg cttgccgact
     7141 tggcaagtgt ggaaaaagcc atcgtccgcg aagaaaaacg cgcccgatca ggcgacaaag
     7201 acgcgcaaaa actagtcgat ttgtgcaaaa aactgctgcc gcatctggac gaaggcaaac
     7261 ccgtgcgttc cttcggtttg gacgcggaag aacgcgcgct gctgaagccg ctgttcctgc
     7321 tgaccgccaa acctgcgatg tatgtgggaa acgttgccga agacggtttt gaaaacaacc
     7381 cgcacctcga ccgcctgaaa gaattggcgg caaaagaaaa cgcccccgtc gttgccgtct
     7441 gcgccgcgat ggagagcgaa atcgccgaat tggaagacgg tgaaaaagcc gaatttctcg
     7501 ccgaaatggg cttggaagaa ccgggcttga accgcctcat ccgcgcgggt tacgaccttt
     7561 tgggactgca aacctacttc accgccggcg tgaaagaagt ccgcgcgtgg acgatacaca
     7621 aaggcgacac cgcgccgcaa gccgccggcg tgatccatac ggattttgaa cgcggcttca
     7681 tccgcgccca agtgattgcc tacgatgatt ttgtctcgct cggcggcgaa gccaaagcca
     7741 aagaagccgg caaaatgcgc gtggaaggca aggaatacgt cgtgcaggac ggcgacgtga
     7801 tgcacttttt gtttaacgtg taacccaaat gcggcaggtt ttcagacggc tttgccggaa
     7861 atgccgtctg aagccggttt tggtggtttt cgacgttccc ataccgccgg aatgcagccg
     7921 catcaaaata aaatcccgcc cgcatttccg atttgccctc cccgattcct gcaaaacaaa
     7981 ccgcctgccc tgccgttacg ggaagccgtc cggtattccg aatatcccga accccgatac
     8041 aaaatgacct ttcagacggc atttgcacag cccgccgcgt ttcaagtaaa aacattatga
     8101 gccaagcctt accctaccgc ccggacatcg acacattgcg cgccgccgcc gtcttgtccg
     8161 tcatcgtgtt ccatatcgaa aaggattggc tgccgggcgg gtttctcggt gtcgatatat
     8221 tctttgtgat ttcaggcttt ttgatgacgg cgatcctcct tcgcgaaatg tccggggggc
     8281 gtttcttcct caagacattt tatatccgcc gcatcaaacg gattttgccc gcatttttcg
     8341 ccgtattggc ggcaacgctg gcaggcggct tctttttatt caccaaagat gatttctttc
     8401 ttttgtggaa atccgcgctg accgccttgg gtttcgcctc caacctgtat tttgcaaggg
     8461 ggaaggatta tttcgatccc gcgcaggaag aaaagcccct gctgcacatc tggtctttgt
     8521 cggtcgaaga acaattttac tttgtctttc cgatattgct gttgcttgtc gcccgcaaaa
     8581 gcctgcgcgt acagttcggc ttcctcgccg cattgtgcgc cttaagcctt gccgcttcct
     8641 ttatgccttc cgcgctcgat aaatattacc tgccccacct gcgcgcctgc gaaatgctgg
     8701 tcggatcgct gaccgccgtg cggatgcggt accggcaaca gcggaatccc gccgtcggga
     8761 aacggtatgc cgccgtcggc gcattgtttt ccgcgtgcat actgtccgcc tgcctgtttg
     8821 cctattcgga acaaaccgcc tatttcccgg gctccgccgc cctgattccc tgtctggctg
     8881 ttgccgcgct gatttatttc aaccattacg aacacccgct taaaaaattt tttccaatgg
     8941 aaaatcaccg ttgccgccgg tttgatttcc tattcgcttt atctgtggca ttggccgata
     9001 ttggccttta tgcgctatat cggcccggac aacctgccgc cttattcgcc ggcggcagcg
     9061 atcgtcctga ccctggcgtt ttccctgatt tcttatcact gcatcgaaaa gccgtttaaa
     9121 aaatggaaag gctcgttcgc acaatccgtt ttatggattt atgccttgcc tatgctcgtt
     9181 ttgggggcgg gctcgttttt cgcgatgagg ctgccgttta tggcgcaata cgaccgcttg
     9241 gggctgacgc gttccaacac ctcctgccac aacaataccg gcaaacaatg cctgtggggg
     9301 gatacggaaa aacagccgga actgctggtt ttgggcgact cccacgccga ccattacaaa
     9361 acattcttcg atgccgtggg caaaaaagaa aaatggtccg ccactatggt ttccgccgac
     9421 gcctgcgcct atgtggaagg ctacgcgtcc cgtgtgttcc aaaactgggc cgcctgccgc
     9481 gccgtttacc gctatgccga agaacacctg ccccggtatc cgaaagtggt tttggcgatg
     9541 cgctggggca gccagatgcc cgaaaacagc cgctcccttg cctacgatgc cggttttttc
     9601 caaaaattcg accgtatgct gcacaaactc tcatccgaaa aacaagccgt ttacctgatg
     9661 gcggacaact tggcttcgtc ttacaacgtc cagcgcgcct atatcttgtc ttcacgcata
     9721 ccgggttgcc gccaaacact gcgcccggac gacgaaagca ccctgaaagc caatgcccgc
     9781 atcagggaat tggcagccaa ataccccaac gtctatatta ttgatgccgc cgcctatatc
     9841 cccgcagatt tccaaatcgg cggattgccg gtttactcgg acaaagacca catcaaccct
     9901 tacggcggca cagaattggc gaagcgtttt tccgaaaaac aaaggtttct cgatacgcgc
     9961 cataaccatt gattcgctta aatttgttac aatcggcggt ttgcaaaacc gctaattttt
    10021 ttgaaagaga ccgatgagcg tcatccaaga cctgcaatcg cgcggcctta tcgcgcaaac
    10081 caccgacatc gaagccttag acgctttgct gaacgaacaa aaaatcgccc tttactgcgg
    10141 tttcgacccg acagccgaca gcctgcacat cggacacctg ctgcccgtat tggcattgcg
    10201 ccgcttccaa caggcggggc atacgccgat tgcactggtg ggcggcgcga ccggtatgat
    10261 cggcgacccc agcttcaaag ccgccgaacg cagcttgaat tccgccgaaa ccgttgccgg
    10321 ctgggtagga agcatacgca gccaattaac ccctttcttg agctttgaag gcggaaacgc
    10381 cgccattatg gcgaacaatg ccgactggtt cggcagcatg aactgcctcg acttcctgcg
    10441 cgacatcggc aagcatttct ccgtcaacgc catgctgaac aaagaatccg tcaaacagcg
    10501 catcgaccgc gacggcgcag gcatttcctt caccgagttc gcctattccc tgctgcaagg
    10561 ctacgacttc gccgagttga acaaacgcca cggcgcggtt ttggaaatcg gcggttccga
    10621 ccagtggggc aacatcaccg ccggtatcga cctgacccgc cgcctgaacc aaaaacaagt
    10681 gttcggtctg accctgcctt tggttaccaa atccgacggt accaaattcg gcaaaaccga
    10741 aggcggcgcg gtgtggctga acgcgaaaaa aacctcgccg taccagttct accagttctg
    10801 gctgaaagtc gccgatgccg atgtgtataa attcctgaaa tactttacct tcctgtccat
    10861 cgaagaaatc ggtgtcgtcg aagccaaaga caaggcaagc ggcagcaagc ccgaagcgca
    10921 acgcatcctc gccgaagaaa tgacccgcct gattcacggc gaagaagccc ttgccgccgc
    10981 gcaacgcatt tccgaaagcc tgtttgccga agaccaaagc cggcttaccg aaagcgactt
    11041 cgagcagctc gccctcgacg gcctgcctgc atttgaagtt tcagacggca tcaacgccgt
    11101 cgaagccttg gtcaaaaccg gcttggcagc gtccaacaaa gaagcgcgcg gctttgtcaa
    11161 tgccaaagcg gttctgctca acggcaaacc ggctgaagcc aacaacccca accacgccgc
    11221 cgaacgcccc gacgatgcct atctgttgat aggcgaatac aaacgtttcg gcaaatacac
    11281 catcctccgg cgcggcaaac gcaaccacgc gcttttggtt tggaaataat ccgattgccg
    11341 cagaaatgcc gtctgaagct ttcagacggc atttttatca aatgcaaaac accctgcgcc
    11401 tgccgatatg tcgtcatttc catgcaggcg ggaattcaaa cttgtccgca cggaaactta
    11461 tcgggcaaaa cggtttcttc agttctacgt tctagatgcc cgctttcgtg ggaatgacgg
    11521 ttcagttgct acggttactg tcaggtttcg gttatgttgg aatttcggga aacttatgaa
    11581 ttgtcattcc cgtgaaagtg ggaatccagg actcaaaatc tcaagaaacc gttttgcccg
    11641 ataagttcct gcactgacag atctagattc ccgcctgcgc gggaatgacg ggtttcgaga
    11701 ttgcggtgtt gtcgggaatg acgggtttta agatt
//
LOCUS       DAWTQS010000062        11646 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.062, whole genome shotgun sequence.
ACCESSION   DAWTQS010000062 DAWTQS010000000
VERSION     DAWTQS010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11646
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.062"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(91..525)
                     /gene="queD"
                     /locus_tag="WNA25_001697"
     CDS             complement(91..525)
                     /gene="queD"
                     /locus_tag="WNA25_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFA9824409.1"
                     /translation="MKITKIFTFDSSHMLDGHDGKCQNLHGHTYKLEITVSDGTIKGG
                     AKDGMVMDFTDLKAIVKQHITDHFDHAFIYHGGNGRESQIAALLEGWNMKTLRLPCRT
                     TAENMAVEMYCRLKNAGLNVCRVKLWETPTSCAEYEGGEGIF"
     gene            complement(613..1128)
                     /locus_tag="WNA25_001698"
     CDS             complement(613..1128)
                     /locus_tag="WNA25_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1543 domain-containing protein"
                     /protein_id="HFA9824410.1"
                     /translation="MPKLHMFYLGGNAGRSNIEVHDIQFAVCDDYREAVPALKAAWFG
                     DTDKIHIDGWQVVEWADGYDIAVSETPKTKMPPENAPRLYFANVGGYRAGQLAEAHAF
                     GLFAAATPAEAKQKALQTLLTDYVRQHKDNLKDVDNLLALEHIGNFHIRLTPNPHGKP
                     AEIGFQGYLPI"
     gene            complement(1156..1815)
                     /gene="queC"
                     /locus_tag="WNA25_001699"
     CDS             complement(1156..1815)
                     /gene="queC"
                     /locus_tag="WNA25_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFA9824411.1"
                     /translation="MSNQKALVIFSGGQDSTTCLIQAIQTYGRENVQAITFRYGQRHA
                     VELERAEWIAQDLGVSQTVLDLSLMRQITHNALMDETAAIETADNGVPNTFVDGRNAL
                     FLLYAAIFAKGQGIRHIIAGVCETDFSGYPDCRGVFVKSMNVTLNLAMDYDFQIHTPL
                     MYLTKAQTWALADEMGVLDYIREQTHTCYKGIVGGCRECPSCILRERGLAECLESKKA
                     V"
     gene            complement(2000..2205)
                     /locus_tag="WNA25_001700"
                     /pseudo
     CDS             complement(2000..2205)
                     /locus_tag="WNA25_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779592.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
     gene            2304..3530
                     /locus_tag="WNA25_001701"
     CDS             2304..3530
                     /locus_tag="WNA25_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HFA9824412.1"
                     /translation="MTFLQRWQGLADNKICAFAWFVIRRFSEERVPQAAASMTFTTLL
                     ALVPVLTVMVAVASIFPVFDRWSDSFVSFVNQTIVPQGADMVFDYIDAFRDQANRLTA
                     IGSVMLVVTSLMLIRTIDNAFNRIWRVNTQRPWMMQFLVYWALLTFGPLSLGVGISFM
                     VGSVQDSVLSSGAQQWADALKTAARLAFMTLLLWGLYRFVPNRFVPARQAFVGALITA
                     FCLETARFLFTWYMGNFDGYRSIYGAFAAVPFFLLWLNLLWTLVLGGAVLTSSLSYWQ
                     GEAFRRGFDSRGRFDDVLKILLLLDAAQKEGRTLSVQEFRRHINMGYDELGELLEKLA
                     RYGYIYSGRQGWVLKTGADSIELSELFKLFVYRPLPVERDHVNQAVDAVMTPCLQTLN
                     MTLAEFDAQAKKQQQS"
     gene            complement(3551..3832)
                     /locus_tag="WNA25_001702"
     CDS             complement(3551..3832)
                     /locus_tag="WNA25_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFA9824413.1"
                     /translation="MIREEISAMPMGYETLIGDMGSALSGGQKQRIVLARALYCEPKI
                     LFLDEAASHLDIANEKAVNANLNGLSIIKIMAAHRKETVESADRKMSLG"
     gene            3911..4027
                     /locus_tag="WNA25_001703"
                     /pseudo
     CDS             3911..4027
                     /locus_tag="WNA25_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(4025..5746)
                     /locus_tag="WNA25_001704"
     CDS             complement(4025..5746)
                     /locus_tag="WNA25_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HFA9824414.1"
                     /translation="MDYLQNLSLGLTKKLPVILQTEVAECGLACLAAVAGFYGFYTDL
                     RALRSKYCLSLKGENLADIVRFADDMGLTGRALRLDLDELGSLRLPCILHWDLNHFVV
                     LESVSSDGAAVMDPASGRRKVKTEEISRKFTGIALELWPNTRFEAGEEKQEIRILPML
                     RGISGLGRTLFQLLALAAAMEVFALVSPFFMQWVIDHVVVTGDRNLLATLALGFTLLL
                     LVQNTVSVMQGWLGMHFSTTLNVQWKSNVFKRLIDLPTDYFAKRHLGDVVSRFGAVDS
                     IQGTLTSTFFVLVLNSLTAVFTFVLMIVYSPILTGLVAATLVVYIAVRWVAYYPLRRA
                     TEENIIHAAKQSSYFMETVRGIRAVKQFGKGPQRYSAWMGLLVDTVNTGLTVQKLGIW
                     FGLANKLLFGLANILIIYLAAGMVLDGVFTVGVLMAFLAYKGQFEGRIGSLVDQFVQI
                     KMLSLHAERLADTVLNETESEATPDVGIPDISDDIEISVENVSFRYADNEPYVLQNVS
                     FKIGRGESLALIGRSGCGKSTFLDILSGNLPPESGKVMINGHDIYSLPPRFIRNLSAM
                     VRQDDVL"
     gene            complement(5848..6063)
                     /locus_tag="WNA25_001705"
     CDS             complement(5848..6063)
                     /locus_tag="WNA25_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824415.1"
                     /translation="MDFAHYLKHWKAAVLIYLAISILTDILCYFLNFDGVFYKGRFFS
                     VTVAGPVGALSFLAYLLYLKREENRSH"
     gene            complement(6077..6424)
                     /locus_tag="WNA25_001706"
     CDS             complement(6077..6424)
                     /locus_tag="WNA25_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824416.1"
                     /translation="MRELTMNEMTAVFGGTEGTWGHRGRVSGEWVGSKVGGALGSFAG
                     AFTGMAGGALVTGGNPAGIAIGGAGGGWVGAEGGSALGGHIGGFAGAKWGKMIDRRNC
                     QCGEDCHCDPCNC"
     gene            complement(6609..7104)
                     /locus_tag="WNA25_001707"
                     /pseudo
     CDS             complement(6609..7104)
                     /locus_tag="WNA25_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237325.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
     gene            complement(7354..7884)
                     /locus_tag="WNA25_001708"
                     /pseudo
     CDS             complement(7354..7884)
                     /locus_tag="WNA25_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234249.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin D"
     gene            complement(7945..8299)
                     /locus_tag="WNA25_001709"
                     /pseudo
     CDS             complement(7945..8299)
                     /locus_tag="WNA25_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234250.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8292..8519)
                     /locus_tag="WNA25_001710"
     CDS             complement(8292..8519)
                     /locus_tag="WNA25_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824417.1"
                     /translation="MKNWKQFIFFVILVIACYQLLYSLSDMFLLDYINKYSWNLNFIQ
                     GTLNFFSIYLPYVFVSRIFRNTNQEKEYKND"
     gene            complement(8528..9682)
                     /locus_tag="WNA25_001711"
     CDS             complement(8528..9682)
                     /locus_tag="WNA25_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFA9824418.1"
                     /translation="MGIHLDFGISPKTFRQTYLYQKPKLFKGAVRNLEAASCKYINEI
                     YQRADPTAPLFHLRKKGAIVPKEEYVESFDDLGKTRYRFIKSVIYEHMKNGASLVYNH
                     INNEPFSDHIARQVARFAGAHTIVSGYLAFGSDESYKNHWDARDMYAVQLFGKKRWQL
                     TAPDFPMPLYMQQTKDTDISIPEHIDMDIILEAGDVLYIPRGWWHRPIPLGCETFHFA
                     VGTFPPNGYNYLEWLMKKFPTIESLRHSFSDWEQDRTRINDTAAQIAAMIADPVNYEA
                     FSEDFLGKERTDTAFHLEQFANPNATPLSDDVRLRLNANNLDTLEKGYLIGNGMKISV
                     DELGKKVLEHIGKNEPLLLKNLLVNFNQAKHEEVRKLIYQLIELDFLEIL"
     gene            9934..10215
                     /locus_tag="WNA25_001712"
     CDS             9934..10215
                     /locus_tag="WNA25_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824419.1"
                     /translation="MKTLVLLLLSFSTTTAFAAYGLGLGQAPKYPAGFRAYGYVYSGR
                     QGWVLKTEADAIKLDTLFKRFVYRPSAANQNPVGQRSDGIPANHTKEKP"
     gene            10212..11255
                     /locus_tag="WNA25_001713"
     CDS             10212..11255
                     /locus_tag="WNA25_001713"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFA9824420.1"
                     /translation="MSKKIKAGIVGATGYTGVELLRLLAAHPDVEVAAVTSRSEAGTA
                     VADYFPSLRGVYGLAFQTPDEAGLEQCDIVFFATPNGIAMKDAPRLIEQGVRVIDLSA
                     DFRIRDIPTWEHWYGMTHAAPGLVSQAVYGLSELNREAVAQARLVANPGCYPTCVSLP
                     LVPLLRQCRLKPGMPLIADCKSGVSGAGRKGNVGSLLCEAGDNFKAYGTAGHRHLPEI
                     RQTIAGLQDGIAEGFVFTPHLAPMIRGMHATVYLHLSDGSDPETVLRDYYRDSPFMDI
                     LPAGSTPETRSVRGANLCRISIRQAAQSDVWVVLSVIDNLVKGAAGQAVQNMNIMFGL
                     EETHGLGGIPLLP"
BASE COUNT         2766 a         3028 c         2844 g         3008 t
ORIGIN      
        1 aatcgttctc tttgatctga ggcgaggcaa cgccgtaccg gtttaaagtt aatccaccat
       61 accttatttg aattgactat atcgatacgt tcaaaatatt ccctcccccc cttcatactc
      121 cgcgcacgat gtcggcgttt cccacaattt gacgcggcac acgttcagcc ccgcgttttt
      181 cagacggcag tacatttcga ccgccatatt ttcggcagtt gtgcggcagg gcaggcgcag
      241 ggttttcatg ttccagcctt ccaaaagcgc ggcgatttga gattcgcgcc cgttgccgcc
      301 gtggtagatg aaggcgtggt cgaaatggtc ggtaatgtgt tgtttcacaa tggctttcaa
      361 gtcggtaaag tccatcacca taccgtcttt cgctccgcct ttgatggtgc cgtctgaaac
      421 ggtgatttcg agtttgtagg tatgtccgtg caggttttgg catttgccgt catgcccgtc
      481 gagcatatgc gaggagtcga aggtaaatat cttggtaatt ttcatcccga ttccttttca
      541 gtgaaaaccc cgccgctttc cgcccggagg gtggcttgat gtgtcgtttt gcgtggaaac
      601 atatccgctg cttcaaatgg gcaaatagcc ttgaaagccg atttcggcgg gtttgccgtg
      661 cggattcggg gtcaggcgga tatggaaatt gccgatgtgc tcgagcgcaa gcaggttgtc
      721 cacgtctttc aagttgtctt tatgctgccg aacatagtcg gtcaacaggg tttgcagggc
      781 tttttgtttg gcttcggcag gcgtggcggc ggcgaacagc ccgaacgcgt gcgcctcggc
      841 aagctgcccc gcgcgatagc cgccgacatt ggcaaaatac aggcgcggag cgttttcagg
      901 cggcattttc gttttgggcg tttcggatac ggcgatgtcg taaccgtccg cccattcgac
      961 aacctgccaa ccgtcgatgt ggattttgtc cgtatcgccg aaccacgcgg ctttgagtgc
     1021 ggggacggct tcgcggtagt cgtcgcacac ggcaaattgg atgtcgtgca cttcgatatt
     1081 cgaccggccg gcattgccgc cgaggtaaaa catatggagc ttgggcatat cgtattcctt
     1141 gtgctttgcg cgcgttcaga cggccttttt actttccaga cattccgcca gcccgcgttc
     1201 gcgcaagata cagctcgggc attcgcggca gccgccgacg atacccttgt agcaggtgtg
     1261 ggtctgttcg cggatgtagt ccaacacgcc catttcgtcc gccaacgccc acgtttgcgc
     1321 cttggtcagg tacatcagcg gcgtgtggat ttgaaaatcg tagtccatcg ccagattaag
     1381 ggtaacgttc atggatttga cgaacacgcc gcggcagtcg ggatagcccg aaaaatcggt
     1441 ttcgcacacg cccgcgatga tgtgccggat accctgccct ttggcaaaaa tggcggcgta
     1501 aagcaggaaa agcgcgttac gcccgtccac aaaggtattg ggaacgccgt tgtcggcggt
     1561 ttcgatggcg gcggtttcgt ccatcagggc gttgtgcgta atctgccgca tcaggctcaa
     1621 atcgagtacg gtttgactga cacccaaatc ctgcgcgatc cactctgcgc gttccagctc
     1681 gacggcatgg cgttgcccgt atcggaaggt gatggcttgg acgttttcgc gcccgtaggt
     1741 ttggattgcc tgaatcaggc aggtggtcga atcctgaccg cccgagaaga tgaccaaggc
     1801 tttttggttt gacatatcga atcctagttt tggtaacgcg ggaggtttgc gaaccgcgaa
     1861 gttggacagt aaagacgcgg attatacagg caaacgcgtt cagacggcat ctgaacgcca
     1921 tgccgtctga acacgcggcg tatgccgact tgacggcgag ttccgccaag cgtctgcctt
     1981 gtgcgaaggc gatattgttt tcttcggcgg tcaggacggg agccgctgtc gggaatatct
     2041 ttatttgacg gcttcgcaga cggcggacac tttggggacg gtgcgcaata cggcttcgca
     2101 accttcaacg ctgtcgatgc cgcgagtgat tcggcgtgcg ggattgcggg tgctgccgtt
     2161 ttgggaatag tagaggacga ggattttgag gggatttggg ttcatgggcg gattccgtta
     2221 aaatagtttg gtgttcagac ggcataacgg ttgtccagat gccgtctgaa acagtttgaa
     2281 aacatgaaac ctggtaagag attatgacct ttttacaacg ttggcaaggt ttggcggaca
     2341 ataaaatctg tgcatttgca tggttcgtca tccgccgttt cagtgaagag cgcgtaccgc
     2401 aggcagcggc gagcatgacg tttacgacac tgctggcact cgtccccgta ctgaccgtaa
     2461 tggtcgcggt cgcttcgatt ttccccgtgt tcgaccgctg gtcggattcg ttcgtctcct
     2521 tcgtcaacca aaccattgtg ccgcagggcg cggatatggt gttcgactat atcgacgcat
     2581 tccgcgatca ggcaaaccgg ctgaccgcca tcggcagcgt gatgctggtc gtaacctcgc
     2641 tgatgctgat tcggacgata gacaatgcgt tcaaccgcat ctggcgggtt aacacgcaac
     2701 gcccctggat gatgcagttc ctcgtttatt gggcgttgct gactttcggg cctttgtctt
     2761 tgggtgtggg catttccttt atggtcgggt cggttcaaga ctccgtactc tcctccggag
     2821 cgcaacaatg ggcggacgcg ttgaagacgg cggcaaggct ggctttcatg acgcttttgc
     2881 tgtgggggct gtaccgcttc gtgcccaacc gcttcgtgcc cgcccggcag gcgtttgtcg
     2941 gagctttgat tacggcattc tgcctggaga cggcacgttt cctgttcacc tggtatatgg
     3001 gcaatttcga cggctaccgc tcgatttacg gcgcgtttgc cgccgtgccg tttttcctgc
     3061 tgtggttaaa cctgctgtgg acgctggtct tgggcggggc ggtgctgact tcgtcgctgt
     3121 cttattggca gggcgaggcc ttccgcaggg gattcgactc gcgcggacgg tttgacgacg
     3181 tgttgaaaat cctgctgctt ctggatgcgg cgcaaaaaga aggccgaacc ctgtccgttc
     3241 aggagttcag acggcatatc aatatgggtt acgatgaatt gggcgagctt ttggaaaagc
     3301 tggcgcggta cggctatatc tattccggca gacagggctg ggttttgaaa acgggggcgg
     3361 attcgattga gttgagcgaa ctcttcaagc tcttcgtgta ccgcccgttg cctgtggaaa
     3421 gggatcatgt gaaccaagct gtcgatgcgg taatgacgcc gtgtttgcag actttgaaca
     3481 tgacgctggc ggagtttgac gctcaggcga aaaaacagca gcagtcttga gtatttttga
     3541 aactgtattt ttatcctaaa gacattttcc tatctgctga ttccaccgtt tcctttctgt
     3601 gtgccgccat aatttttata atagacaagc cattcaagtt tgcattgact gctttttcat
     3661 tggcaatatc caaatgactg gccgcttcat ctaaaaatag gattttcggt tcgcaatata
     3721 aggcccgcgc caatacgata cgttgttttt gtccgcctga cagtgcgctg cccatatcgc
     3781 cgatcaaggt ttcatagccc ataggcatgg cggagatttc ttcgcgaatc atagcaaggc
     3841 aggcgcaatg ttcgattttt cctctatccg gttctgtatc gaaaaaacag atattctctg
     3901 caattgagcc agcaaatgca aatatagtgg attaaattta aatcaggaca aggcggcgaa
     3961 gccgcagaca gtacagatag tacggcaagg cgaggcaacg ccgtaccggt ttaaatttaa
     4021 tccactataa aacatcgtcc tgcctgacca tcgcactcaa attgcggata aaacgtggcg
     4081 gtaagctgta aatgtcgtgc ccatttatca tgactttgcc tgattcggga ggtagattgc
     4141 cgcttaaaat atccaaaaat gtcgatttac cgcagcccga tcgtccgatt aacgcaagcg
     4201 attcaccacg tccgatcttg aagctgacgt tttgtaaaac atatggctcg ttatcggcat
     4261 aacggaaaga aacattttcc actgagattt cgatgtcgtc tgaaatatca ggaatgccta
     4321 catccggcgt agcttccgat tctgtttcat tcaatacggt gtcagctaac cgttccgcat
     4381 gaagtgacag cattttaatt tgcacaaact gatcgaccag cgagccaata cgtccttcaa
     4441 actgtccctt ataagccaaa aaagccatta gtacgccgac ggtaaagacg ccgtccaata
     4501 ccatgcccgc agctaagtaa ataatcaaaa tatttgccaa accgaaaagc agcttgtttg
     4561 ccaagccgaa ccaaattccc agtttctgca ccgtcagtcc cgtattaacc gtatccacca
     4621 gcagccccat ccatgcagaa tatcgttgag gacctttacc aaattgcttg actgctcgga
     4681 taccgcgtac cgtttccata aaatagctgc tttgtttggc agcatggatg atgttttcct
     4741 ccgtcgcccg tcgcaacgga taataggcta cccaacggac tgcgatataa actaccagtg
     4801 tcgctgcaac caaacctgtc agtatcgggc tgtacactat cattagcaca aaagtaaaaa
     4861 cagccgttag gctgtttaaa accaacacga aaaacgttga agtcagagtt ccctgaatac
     4921 tatccaccgc cccgaagcgc gataccacat cgcccaaatg ccgtttagcg aaataatctg
     4981 taggcaaatc tatcaaacgt ttgaaaacgt ttgatttcca ctgaacattt aacgtcgttg
     5041 aaaaatgcat gcccaaccag ccctgcatga cagacaccgt attttgtacc agaagtagca
     5101 aggtaaagcc caaagccagc gtcgctaaca gattacgatc gcccgttacc actacatggt
     5161 cgatgaccca ttgcataaaa aacggcgaca ccaaagcaaa cacttccatt gctgctgcca
     5221 aagccaaaag ctgaaacaat gtccgcccca gcccagaaat cccgcgcaac atgggtagga
     5281 tgcggatttc ctgcttttct tcccctgcct cgaaacgcgt gtttggccac agttccaaag
     5341 caattcccgt aaacttgcgc gatatttcct ccgtcttgac tttgcgtcgt cccgaagccg
     5401 gatccatgac ggcagccccg tccgaagata ccgattccag caccacaaaa tgattcaaat
     5461 cccaatgtag aatacagggc aggcgcaaac tgcccaattc gtctaaatcc agcctcaacg
     5521 cccgtcccgt cagccccata tcatcagcaa aacgaacaat atctgccaaa ttctcaccct
     5581 taagtgacag acagtatttt gaacgcagtg cgcgcaaatc cgtatagaaa ccataaaatc
     5641 cggccacagc cgctagacat gccaagccac actccgctac ttctgtttgc agtataacgg
     5701 gcagcttttt tgtcaagccc aaagacaggt tttgcagata atccatagat ttccttgttg
     5761 aatcagaaaa gagagatggg aagacgggaa tggtttggaa aggccgtctg aaaaggtttc
     5821 agacggcctg cagccccata ttgactttca atgcgaccgg ttttcctcgc gtttcaaata
     5881 cagcagatac gcaagaaaag acaaagcccc tacaggtcct gcgacggtaa ccgaaaaaaa
     5941 cctgcctttg taaaacacac cgtcgaagtt taaaaaatag caaaggatgt cggtcaggat
     6001 gctgatggca agataaatca gcacagcggc tttccagtgt ttcaggtaat gtgcgaaatc
     6061 catttcaaac tcccgatcaa cagttgcacg ggtcgcaatg gcagtcttct ccgcattggc
     6121 agttgcgcct gtcgatcatt tttccccatt ttgcaccggc aaaaccgccg atatgcccac
     6181 ccaatgcaga accgccctca gctcctaccc atccgccgcc tgcgccgccg atggcaattc
     6241 cggcaggatt tccgcctgtt accaatgcgc cgcccgccat tcccgtgaat gctccggcaa
     6301 acgaccctaa agcgccgccg accttgcttc cgacccattc tccggagact ctgcccctgt
     6361 gcccccaagt accttctgtg ccgccgaaaa cagcggtcat ttcgttcatg gtcaattctc
     6421 tcatttcaaa actcctttta tcgtcaatcg aaaaattgaa agggcagata ccctatcctt
     6481 gcttttacag acagagtatc tgtaaaaggg ggtagtttca ggcagcttca gtgtttatgt
     6541 ttttatgtag tgttttacag gtcgtctgaa gaggtttcag acgacttttt atctttcagc
     6601 ctcaagcctt acaacctgcc cgacatacta taaatcggct ccaataccca ttcgtacagc
     6661 cgccgtttct cgtgtaggat gtctgcttcc aacgtcatgc cgatttgcag cggtttttcc
     6721 tcaccgtatg cagtgatggt tggtttgtcg ggttttattt tcacgagata aacaggttcg
     6781 ttgctcttcg ccaaatcgga ggataccatg cccaatcccg acaattcctg tctgcccagt
     6841 gccgtttttg ccactgatac gacactgccg gaagcaagcc cgaatttttg atagggatat
     6901 gcctgataac gcaggacaac cttgtctttc ggcttgataa agcctgctgc actgctgggg
     6961 gatatataga tgggcatata actcggtacg ttcgggaaca atgctcaaga acagtttgga
     7021 aggatcaacc tgccgtccga cttcgacgtt cggtattgct atataacccg accgtcctgc
     7081 acggatgatt tgttcagagc gcatttcaaa atccaaaact tcttgagaaa tatcggcaat
     7141 ggtgcgttca agctggcttt gttctgtctc atgccgtttg gggaggctgg ccaatgtcag
     7201 attctgcgtg cggatttcct gaagcagccc ggcttcttct cggcggtagg catcaagttt
     7261 ggctttctgc tctaaaagct ctgcctcgac attcatcatt tcttgttttg acactgcatc
     7321 attggcggat aggaaacgat atttccgcaa catttcttcc gcaagtctaa tgcgcctttt
     7381 ctggccgtct atctgttgcg aaatatggag tttctggttt tccaaacgtt cgacagttgc
     7441 tttaaggctg cgcgtttcat tctcgtgtat cagcttcaga cgacccagtt cctgttctgc
     7501 caacgttttc ttcaaaactg cctccgtttt caactgctgc tgcacgctac ctcctgcgcc
     7561 gaaacgtgag gtcgaaagcg caaatagctt gtcgccagcc ttaacctttt ctccatcttc
     7621 cacgaatttc gctgtaattg tccccgtatc cggtgcatac accctgatta cgcccgatgc
     7681 aggtagaatt tgtccctcca ttgttgtctt tcgcgtatag ttaccgaata tcaaaaacag
     7741 gataatcagt aacgcagata tcgatgcaaa tgtcgtccac agggaaaatg acaacgggcg
     7801 tgtcagaatc actttacccg tcaggctggt ttggcgggca atggcgactt cagggcggaa
     7861 gaaaggttgc ttgggtctat tcataaaatt gaagttaaga aagtttcaga cgacccctag
     7921 agatcgtctg aacaatgaga aatatcagca gtaatctgta ccgtcagtgt agccgtttcc
     7981 tgatttatct gcttttgttg cgggagcagt taatccatgt tcaatctcaa agattggtct
     8041 tccgttataa ggaggtgcat taacggcatc atttacccaa tcacgagtca gattgtatac
     8101 accatttgca ccatatagca ccgaaaaaat ttttcggcag gaaatgaact gtcgctgcag
     8161 cagcaggtat tgcaacaaaa tccccccatg tgggacctcc tttggttgtg gcagcattag
     8221 ctacatttcc agctatattg tctgttacag gacctccccc tgaaaccagc ttcaattcat
     8281 gaagttgaag ttcaatcatt tttatactcc ttttcttggt tggtattcct aaaaattcgg
     8341 ctaacaaaaa catatggcag atatattgaa aaaaaattca aagtaccctg aataaaattc
     8401 aaattccaac tatatttgtt aatgtagtcg agaagaaaca tatctgataa agaatatagc
     8461 aattgataac aagctattac taatattacg aaaaatataa actgcttcca gtttttcata
     8521 gaatccctca caaaatttcc agaaaatcta actctatcaa ctgatagatc aacttcctaa
     8581 cttcttcatg ttttgcctga ttgaagttaa ccagtagatt tttcaacaat aacggttcat
     8641 tcttaccgat gtgttctaac acttttttcc ccaactcatc tacgcttatc ttcatcccat
     8701 tcccaatcaa atatcccttt tccaacgtat ccaaattatt ggcatttagt ctcaacctga
     8761 cgtcgtctga aagcggagta gcgttgggat tcgcgaactg ttcgagatga aaagcggtat
     8821 cggtacgttc tttgccgaga aagtcttcac tgaaagcttc gtaattgacg gggtcggcaa
     8881 tcatggcggc aatttgtgcg gcagtatcgt tgatacgcgt cctatcttgc tcccagtctg
     8941 agaaactgtg gcgcagactt tctatggtgg gaaatttctt cattagccac tcgaggtaat
     9001 tatagccgtt tggtgggaag gtaccgacag cgaagtggaa ggtttcacag ccgagcggga
     9061 taggtctgtg ccaccaaccg cgtgggatgt agaggacatc acctgcttca aggataatat
     9121 ccatatcgat atgttcagga atggaaatat cagtatcttt agtctgttgc atatacaatg
     9181 gcatagggaa atcaggggca gtaagttgcc aacgtttctt gccgaaaagc tggacggcat
     9241 acatatctcg ggcgtcccaa tggtttttat aagattcgtc gctgccaaaa gcaagatatc
     9301 cactaacaat agtatgtgcg ccggcaaagc gggcgacttg acgggcgata tggtctgaaa
     9361 acggctcgtt gttaatatgg ttatagacta acgacgcacc gttcttcata tgttcgtaga
     9421 taacggattt aataaaacgg tagcgagttt tgcccaaatc gtcgaaactt tcgacgtatt
     9481 cttctttagg aacgattgcg ccttttttac gcagatgaaa cagcggtgcg gttgggtctg
     9541 ctcgttggta tatctcgttg atatatttac aagatgcggc ttcgagattc cgaaccgctc
     9601 ctttaaagag cttgggcttt tgatacagat aagtctgtcg gaacgtttta ggactaatgc
     9661 cgaagtcgag atggatgccc attacttccc cttactcaga aaatatttaa aatttataat
     9721 gttacatata gttacaaata ttagagtttt ttgtgtgtgc gtcaaggaat tgttgacaat
     9781 tttagttaaa aatttgtctc caaacggaaa aaagcggttt gttttttgtt gttaacattt
     9841 ttattgtaat taaataaaag gtcgtctgaa aacggttttc agacgacctt ttgctataat
     9901 cgggcttcat cgccccgttc ggtttggaac cttatgaaaa ccctcgtcct cctcctgctt
     9961 tccttctcca cgaccaccgc tttcgccgca tacggtttgg gtttggggca ggcaccgaaa
    10021 tatcctgccg gctttcgcgc ctacggttat gtttattccg gacggcaggg ctgggtttta
    10081 aaaacagagg cggatgccat taaattagac acgcttttca aacgctttgt gtaccgtcct
    10141 tccgccgcca atcaaaaccc cgtcggacag cgttcggacg gcatacccgc caaccacaca
    10201 aaggaaaaac catgagcaaa aaaatcaaag ccggcattgt cggcgcgacg ggctacacgg
    10261 gcgtggaact gctgcgcctg cttgccgccc atccagatgt cgaagtcgcc gccgtaacca
    10321 gccgcagcga agcgggaacc gcagttgccg attactttcc aagtctgcgc ggcgtgtacg
    10381 gcctcgcctt ccaaacgccc gacgaggcag gtttggaaca atgcgacatc gtcttcttcg
    10441 ccacgcccaa cggcatcgcc atgaaagacg cgccgcgcct gattgaacag ggcgtgcgcg
    10501 tcatcgacct ttccgccgac ttccgcatac gggacattcc gacctgggaa cactggtacg
    10561 gcatgaccca cgccgccccc ggcctcgttt cccaagccgt gtacggattg agcgaactca
    10621 accgcgaagc cgtcgcacag gcgcgcctcg tcgccaaccc cggctgctac ccgacctgcg
    10681 tatccctgcc gctcgtgccg ctgttgcggc aatgccgtct gaagcccggt atgccgctga
    10741 ttgccgactg caaatccggc gtgtccggcg cgggcaggaa aggcaatgtc ggttcgctgt
    10801 tgtgcgaagc cggcgacaac ttcaaagcct acggcacagc cggacaccgc cacctgcccg
    10861 aaatcaggca gaccatcgcc gggcttcagg acggcatcgc cgaaggattc gtgttcacgc
    10921 cgcacctcgc gccgatgata cgcggtatgc acgccaccgt ttacctccac ctttcagacg
    10981 gcagcgaccc cgaaaccgtc ctgcgcgact actaccgcga cagcccgttc atggacatcc
    11041 tgcccgccgg ttcgacaccc gaaacccgca gcgtgcgcgg cgcaaacctc tgccgcatca
    11101 gcatccgtca ggcggcgcaa tccgatgtgt gggtcgtcct ttccgtcatc gacaacctcg
    11161 tcaaaggcgc ggcgggacag gcggtgcaaa atatgaacat tatgttcgga ctggaggaaa
    11221 cacacggctt gggcggcatc cccctgctcc cctgaagcgc aaacggcaaa ccgcaggcat
    11281 cgtgcctgcg gttttttgat gccgtctgaa agcggcggtt tttttgggtt cggacggctt
    11341 ttggctcatc attccgcgaa aacaaaaatc taaaataccg tcattcccgc aaaagcggga
    11401 atctagttta tccggcttca gcaatttccg acacatttcc acacgcttcg attccgtcat
    11461 ttctccggtt tcaatcgttt ccgataacac cgtggttttt catttctaga ttcccgcctg
    11521 cgcgggaatg acggcggggg gcttgccgtt ttcccgataa gcctctgcgg cttctcattg
    11581 ccggatttcc gcccgcttgg gaataacggg atttaaaatc atgaaaatgt gtcaaaaata
    11641 atatag
//
LOCUS       DAWTQS010000063        11010 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.063, whole genome shotgun sequence.
ACCESSION   DAWTQS010000063 DAWTQS010000000
VERSION     DAWTQS010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11010
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.063"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(54..1082)
                     /gene="trpD"
                     /locus_tag="WNA25_001714"
     CDS             complement(54..1082)
                     /gene="trpD"
                     /locus_tag="WNA25_001714"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HFA9824421.1"
                     /translation="MITPQQAIERLISNNELFYDEMTDLMRQMMSGKVPPEQIAAILT
                     GLRIKVETVSEITAAAAVMCEFASKVPLEDADGLVDIVGTGGDGAKTFNISTTSMFVA
                     AAAGAKVAKHGGRSVSSSSGAADVMEQMGANLNLTPEQIAQSIRQTGIGFMFAPNHHS
                     AMRHVAPVRRSLGFRSIFNILGPLTNPAGAPNQLLGVFHTDLCGILSRVLQQLGSKHV
                     LVVCGEGGLDEITLTGKTRVAELKDGKISEYDIRPEDFGIETRRNLDEIKVANTQESL
                     LKMNEVLDGKEGAARDIVLLNTAAALYAGNIAASLSDGISAAREGIDSGRAKAKKEEF
                     VGFTRQFA"
     gene            complement(1146..1736)
                     /locus_tag="WNA25_001715"
     CDS             complement(1146..1736)
                     /locus_tag="WNA25_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HFA9824422.1"
                     /translation="MLLFIDNYDSFTYNIVQYFAELGQEVAVRRNDDITLEEIEALNP
                     QYLVIGPGPCSPKEAGISVEAMRHFAGRLPIMGVCLGHQTIGEAFGGDVVRAKTLMHG
                     KVSPVSHSGKGMFKGLPNPVTCTRYHSLVIERGTLPDCLEITAWTEDGEIMGVRHKEY
                     AVEGVQFHPEALLTERGHDMLNNFLVEFQNFKPQKI"
     gene            2147..4423
                     /locus_tag="WNA25_001716"
     CDS             2147..4423
                     /locus_tag="WNA25_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFA9824423.1"
                     /translation="MAQITLKPIVLSILLINTPLLAQAHETEQSVGLETVSVVGKSRP
                     RATSGLLHTSTASDKIISGDTLRQKAVNLGDALDGVPGIHASQYGGGASAPVIRGQTG
                     RRIKVLNHHGETGDMADFSPDHAIMVDTALSQQVEILRGPVTLLYSSGNVAGLVDVAD
                     GKIPEKMPENGVSGEAGLRLSSGNLEKLTSAGINIGLGKNFVLHTEGLYRKSGDYAVP
                     RYRNLKRLPDSHADSQTGSIGLSWVGEKGFIGAAYSDRRDRYGLPAHSHEYDDCHADI
                     IWQKSLINKRYLQLYPHLLTEEDIDYDNPGLSCGFHDGDGAHAHTHNGKPWIDLRNKR
                     YELRAEWKQPFPGFEALRVHLNRNDYHHDEKAGDAVENFFNNKTHNARIELRHQPIGR
                     LKGSWGVQYLGQKSSALSAIPETVQQPMLIDNNVRHYSFFGVEQANWDNFTLEGGVRV
                     EKQKASIRYDKALIDRENYYNQPLPDLGAHRQTARSFALSGNWYFTPHHKLSLTASHQ
                     ERLPSTQELYAHGKHVATNTFEVGNKHLNKERSNNIELALGYEGDRWQYNLAAYRNRF
                     GNYIYAQTLNDGRGPKSIEDDSEMKLVRYNQSGADFYGAEGEIYFKPTPRYRIGVSGD
                     YVRGRLKNLPSLPGREDPYGKRPFIAQDDQNAPRIPAARLGFHLKTSLTDRIDANLDY
                     YRVFAQNKLARYETRTPGHHMLNLGANYRRNTRYGEWNWYVKADNLLNQSVYAHSSFL
                     SDTPQMGRSFTGGVNVKF"
     gene            complement(4963..5742)
                     /locus_tag="WNA25_001717"
     CDS             complement(4963..5742)
                     /locus_tag="WNA25_001717"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HFA9824424.1"
                     /translation="MNRLYPHPIIAREGWPIIGGGLALSLLVSMCCGWWSLPFWVFTV
                     FALQFFRDPAREIPQNPEAVLSPVDGRIVVVERARDPYRDVDALKISIFMNVFNVHSQ
                     KSPADCTVTKVVYNKGKFVNADLDKASTENERNAVLATTASGREITFVQVAGLVARRI
                     LCYTQAGAKLSRGERYGFIRFGSRVDVYLPVDAQAQVAIGDKVTGVKTVLARLPLTDS
                     QADPVSQAASVETAANPSAEQQQIEAAAAKIQAAVQDVLKD"
     gene            5896..8745
                     /gene="uvrA"
                     /locus_tag="WNA25_001718"
     CDS             5896..8745
                     /gene="uvrA"
                     /locus_tag="WNA25_001718"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFA9824425.1"
                     /translation="MCNHHPRHSHDNDTIRIRGARTHNLKNIDLDIPRHKLVVVTGLS
                     GSGKSSLAFDTLYAEGQRRYVESLSAYARQFLQMMDKPDVDLIEGLSPAISIEQKSTS
                     HNPRSTVGTVTEIHDYLRLLYARVGTPYCPEHKLPLSSQTVSQMVDAVLKLPEDTRVM
                     ILGPAVRERKGEFVDFFADLQAQGFARVRVDGEVYQLDEVPKLEKNIKHNIDVVIDRV
                     KVKADIKQRLAESFETALRHGNERALAMEMDSGEEHWFSARFACPVCSYSLPELEPRL
                     FSFNNPMGSCPTCDGLGNTNFFDPEKVVAHPELSLATGAIDGWDKRNQFYFQMIQSLA
                     HHYKFDVNVAWETLPEKVKKVVLHGSGKEVIDFTYLSERGTTFNRSHAFEGIIPNLER
                     RYRETDSETVREKLREYQNHRACPSCGGARLRKEARYVYVGGEPLHEVSAWPLTKTHR
                     FFETLDLDGNKKQIAEKILKEITERLGFLINVGLDYLNLSRSAETLSGGEAQRIRLAS
                     QIGSGLTGVMYVLDEPSIGLHQRDNDRLLATLKRLRDLGNSVIVVEHDEDAIREADFV
                     VDMGPGAGEHGGNVLIADTPENVAKCEKSVTGQYLGGKKSIAVPSERTPVNPGRMLVL
                     KGARGNNLKNVTLELPLGLITCITGVSGSGKSTLINDTLAKITARELNRAQEEPAPYD
                     DIRGLEHLDKVINVDQSPIGRTPRSNPATYTGLFTPIRELFAGVPLSRERGYNVGRFS
                     FNVKGGRCEACQGDGVIKVEMHFLPDVYVPCEVCHGKRYNRETLEIQYKGKNISQVLD
                     MTVEEAREFFDAVPTVSRKLQTLMDVGLGYIRLGQSATTLSGGEAQRVKLALELSKRD
                     TGRTLYILDEPTTGLHFADIALLLEVIGRLKGKGNSIVIIEHNLDVIKTADWIVDLGP
                     EGGDGGGKVIAKGSPEQVAKIKGSYTGKYLKVVLK"
     gene            8763..9404
                     /locus_tag="WNA25_001719"
     CDS             8763..9404
                     /locus_tag="WNA25_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eco29kI family restriction endonuclease"
                     /protein_id="HFA9824426.1"
                     /translation="MNNNFDRSKHIYHNEAFVELVKDAIRFFHGTPVHTLPPPKQFKG
                     AGVYAIYYIGNNPLYKQYADWNRLSYNAPIYVGKAVPKGWRQARNSDNALNQSTELFH
                     RLKEHSRSIAAVSDLDPSDFMCRFVIFEGAGSNMIGTIEAALIKLHKPLWNSCVDGFG
                     NHDPGKGRYEQAKSDWDVLHSGRVWADRLNGIPNSYESILENINTHLEIIKRK"
     gene            9408..10541
                     /gene="dcm"
                     /locus_tag="WNA25_001720"
     CDS             9408..10541
                     /gene="dcm"
                     /locus_tag="WNA25_001720"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HFA9824427.1"
                     /translation="MKSLEIFSGAGGLAKGLELAGFQHASFIELNKDACNSLRSNFNP
                     KLVYQGDVADFDLSSQEGIEVIAGGPPCQPFSLGGKHLAHEDRRDMFPHAVRYVEYYR
                     PKAFIFENVKGLLRKSFADYFEYILLRLTYPNLGILQNEDWKGHLTRLKEIEFNLYKG
                     IKYKVSYQLLNAADYGVPQKRERVVIVGIRADLDIDWKFPKRTHSEDRLNWEKYVTGE
                     YWEKHNEPKRFNKDIAEKLQKKYGIFEPEKKPWQTVRDTLSDIPHPLGNHKITGHEYR
                     DGARIYPGHTGSGIDEPSKTIKAGGHGVPGGENMIRYDDGTVRYFTSYEAKLLQTFPE
                     EFVISGAWGEAMRQIGNAVPVKLSEILGKHLMGVLSEKSSLHN"
     gene            10849..>11010
                     /locus_tag="WNA25_001721"
     CDS             10849..>11010
                     /locus_tag="WNA25_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFA9824428.1"
                     /translation="MDVQKQYPFETVAVCVLPNHIHAIWTLPPDDADYSLLRRLIKTK
                     FSAYSPHTKK"
BASE COUNT         2909 a         3099 c         2555 g         2447 t
ORIGIN      
        1 cgccaattcc gccgttcaga cggcatttgt tgctttatat caagtttgcc ggcttaggcg
       61 aattgccgtg taaaaccgac aaactcctct tttttcgcct tcgccctacc tgaatcgatg
      121 ccttcccgtg cggcagatat gccgtctgaa agcgaagcag cgatatttcc ggcatataag
      181 gcggcggcgg tattaagcaa tacgatatcg cgcgccgccc cttctttccc gtccagtacc
      241 tcgttcattt tcaacaaaga ttcctgagta ttggcaactt tgatttcatc caaattgcgg
      301 cgggtttcga taccgaaatc ttctgggcgg atgtcgtatt cgctgatttt tccgtctttg
      361 agttcggcaa cgcgcgtttt gcccgtcagt gtaatttcat ccaaaccgcc ctccccgcaa
      421 acgaccaaaa cgtgttttga gccgagttgt tgcaagaccc gcgacaaaat gccgcacaaa
      481 tcggtgtgga acacgcccaa aagctggttc ggcgcgcccg caggattggt taacggaccc
      541 aatatgttga aaatgcttcg gaaaccgagc gaacggcgca ccggggcgac atggcgcatg
      601 gcactgtggt gattgggtgc gaacataaac cctataccgg tctgcctgat actttgggca
      661 atctgttcgg gagtcaggtt gaggtttgcg cccatttgct ccatcacgtc agccgcaccg
      721 ctggaggaag agaccgaccg gcctccgtgt ttggcaacct ttgcgcctgc cgctgcagca
      781 acaaacatcg aagtcgtcga aatattgaag gttttcgcgc catccccgcc cgtaccgacg
      841 atatcgacca gcccgtccgc atcctccagc ggcacttttg acgcaaactc gcacatcacg
      901 gcggcggcgg cggtgatttc ggaaacggtt tcaaccttga tacgcaagcc ggtcaaaatc
      961 gccgcaattt gttcgggcgg cacttttccg ctcatcattt ggcgcatcaa gtcggtcatt
     1021 tcatcgtaaa acaactcgtt attgctgatt aatcgttcga tagcctgttg cggtgtaatc
     1081 attttttgtc ctccgttcaa tattcggacg aaaatgccgc ccgaagggct tcagacggca
     1141 tcacgtcaga ttttttgcgg tttgaagttt tgaaattcaa ctaaaaaatt gttcaacata
     1201 tcatgtccgc gttcggtcaa gagggcttcg gggtggaact gcacgccctc gacggcatat
     1261 tccttatggc gcacgcccat aatttcgccg tcttccgtcc acgccgtgat ttccaagcaa
     1321 tccggcagcg tgccgcgttc gataacgagg ctgtgataac gcgtacaggt aaccggattg
     1381 ggcaaaccct taaacatacc cttgcccgaa tgggacacgg gcgacacctt accgtgcatc
     1441 aaggtttttg cccgtaccac atctccaccg aacgcttcgc ctatcgtctg atgcccgagg
     1501 cacacgccca taatcggcag ccggccggca aaatggcgca tggcttctac tgaaataccc
     1561 gcctccttag gggaacacgg tccgggaccg ataacgagat attgcggatt caatgcctcg
     1621 atttcctcca acgtaatatc atcgttgcgg cgcacggcga cttcctgccc caattctgcg
     1681 aaatactgga cgatgttgta agtaaaactg tcgtaattgt cgataaacaa aagcattttg
     1741 tgattaacct attgatttat aatttgttta ttattaactg catttccgat acccccatcc
     1801 ataccccctt atatcttagc gtgcccgatg cgccctcgtg aatctggcgc agagcctcgc
     1861 gttctgacaa gtacgccatt ttgccacctt ttcgaccgtt cgggaacgta ccgtttttat
     1921 gccctcataa attttggtta tccgatagct aacatatagc ggcgttcgtg ttcacatcat
     1981 taacttccct aaaattaggg ttaatacaaa ttaacgccat taaatatcac aaccgtgtca
     2041 tttgaaaaac ttttgcctat gccctctgag tattctcaaa atcttatgat ataaaaaatg
     2101 caatgttata taataacaaa cttttgaaat gcgacgagaa gccaaaatgg cacaaatcac
     2161 actcaaaccc attgttttat caattctttt aatcaacaca cccctcctcg cccaagcgca
     2221 tgaaactgag caatcggtgg gcttggaaac ggtcagcgtc gtcggcaaaa gccgtccgcg
     2281 cgcgacttcg gggctgctgc acacttcgac cgcctccgac aaaatcatct ccggcgatac
     2341 tttgcgccaa aaagccgtca acttgggcga cgctttggac ggcgtaccgg gcattcatgc
     2401 ctcgcaatac ggcggcggcg catccgctcc cgttattcgc ggtcaaacgg gcagacggat
     2461 taaagtattg aaccatcacg gcgaaacggg cgatatggcg gacttttctc ccgatcacgc
     2521 cattatggta gataccgcct tgtcgcaaca ggttgaaatc ctgcgcgggc cggttacgct
     2581 cttgtacagc tcgggcaatg tggcggggct ggtcgatgtt gccgatggaa aaatccccga
     2641 aaaaatgcct gaaaacggcg tatcgggcga agccggattg cgtttgagca gcggcaattt
     2701 agaaaaactg acatccgcag gcatcaatat cggactgggc aaaaacttcg tgctgcatac
     2761 cgaaggcttg taccgcaaat cgggcgatta cgccgtaccg cgttaccgca atctgaaacg
     2821 cctgcccgac agccatgccg attcgcaaac gggcagcatc gggctgtctt gggtaggcga
     2881 aaaaggcttt atcggcgcag catacagcga ccgtcgcgac cgctacggcc tgcctgccca
     2941 cagccacgaa tacgatgatt gccacgccga catcatctgg caaaagagtt tgatcaacaa
     3001 acgctatttg cagctttatc cgcacttgtt gaccgaagaa gacatcgatt acgacaatcc
     3061 gggcttgagc tgcggcttcc acgacggcga cggtgcacac gcacacaccc acaacggcaa
     3121 accgtggata gacctgcgca acaaacgcta cgaactccgc gccgaatgga agcagccatt
     3181 ccccggtttt gaagccctgc gcgtacatct gaaccgcaat gactaccacc acgacgaaaa
     3241 agcaggcgat gcagtagaaa acttcttcaa caacaaaaca cacaacgccc gtatcgagtt
     3301 gcgccaccaa cccataggcc gtctgaaagg cagctggggc gtgcaatatt tgggacaaaa
     3361 atccagcgcg ctttccgcca ttcccgaaac cgtccaacaa ccgatgttga ttgacaacaa
     3421 tgtccgccat tacagctttt tcggtgtaga acaggcaaat tgggacaact tcacgcttga
     3481 aggcggcgta cgcgtggaaa aacaaaaagc ctccatccgg tacgacaaag cattgattga
     3541 tcgagaaaac tactacaacc agcccctgcc cgacctcggc gcgcaccgcc aaaccgcccg
     3601 ctcgttcgca ctttcgggca actggtattt cacgccacac cacaaactca gcctgaccgc
     3661 ctcccatcag gaacgcctgc cgtcaacgca agaactgtac gcacacggca agcacgtcgc
     3721 caccaacacc tttgaagtcg gcaacaaaca cctcaacaaa gagcgttcca acaatatcga
     3781 actcgcgctg ggctacgaag gcgaccgctg gcaatacaat ctggcagcct accgcaaccg
     3841 attcggcaac tacatttacg cccaaacctt aaacgacgga cgcggcccca aatccatcga
     3901 agacgacagc gaaatgaagc tcgtgcgcta caaccaatcc ggtgccgact tctacggcgc
     3961 ggaaggcgaa atctacttca aaccgacacc gcgctaccgc atcggcgttt ccggcgacta
     4021 tgtacgaggc cgtctgaaaa acctgccgtc cctacccggc agggaagatc cctacggcaa
     4081 acgtcccttc atcgcgcagg acgaccaaaa cgccccccgc attccggctg cgcgcctcgg
     4141 cttccacctg aaaacctcgc taaccgaccg tatcgatgcc aatttggact actaccgcgt
     4201 gttcgcccaa aacaaactcg cccgctacga aacgcgtacg cccggacacc atatgctcaa
     4261 cctcggtgca aactaccgcc gcaatacgcg ctatggcgag tggaattggt acgtcaaagc
     4321 cgacaacctg ctcaaccaat ccgtttacgc ccacagcagc ttcctctctg atacgccgca
     4381 aatgggccgc agctttaccg gcggcgtaaa cgtgaagttt taaaatcgga caggcaaaca
     4441 aaaaaggttt tcagacggca ttctgaaaac ctttttttct ttttaatcaa cttgttagga
     4501 aatatggcaa agacaaggaa caaaccactg tgccatcatt cccattttct ttgtttcatc
     4561 attcttgctt tcacgccgtc attctcgcaa aagcagaaaa atcaaaagca aaaacccaag
     4621 atcccatcat tcccgcgcag gcgggaatcc aattcattcg gtttcagccg tttccgataa
     4681 atcaccgtaa cgttaagttc ccggattccc actttcgtgg gaatgacggc gtaaaggttt
     4741 ctgtttttta agttttaggt aactaatgag tcgtcattcc cgcgcaggcg ggaatccaat
     4801 tcgttcggtt tctgttattt ctgataaatc accgtaacgt taagttcccg gattcccatt
     4861 ttcgtgaaca taacggtgtg aacattagca aaaattcaaa aaaccaaact tcctcattcg
     4921 tgtgaatgat ggtactgata ttttctattt cagttcgcaa aattaatctt tcaacacatc
     4981 ttgcacagcc gcctgaatct tagccgccgc cgcttcgatt tgctgctgtt cggcagatgg
     5041 gtttgccgct gtttcaaccg aagcagcttg tgaaacagga tcggcttgag aatcagtcag
     5101 cggcaaacgc gccaatacgg tttttacgcc ggttacttta tcaccaatcg ccacttgcgc
     5161 ctgcgcatcg acaggcagat acacatccac gcgcgaaccg aagcggataa agccatagcg
     5221 ttcgccgcgg gacagtttcg cacctgcttg ggtgtaacac aaaatacggc gtgccaccag
     5281 accggccact tgaacaaaag taatttcacg accggaagcc gtagtcgcca aaaccgcgtt
     5341 gcgttcgttt tccgtgctgg ctttgtccaa atccgcattc acgaatttgc ctttgttata
     5401 gaccactttc gttaccgtac aatcggcagg cgatttttgc gaatgcacgt tgaacacgtt
     5461 cataaaaata ctgattttca aagcatcgac atcacgatac ggatcgcgtg cgcgctcgac
     5521 caccacgata cggccgtcaa ccggactcaa caccgcttca ggattttgcg gaatttcacg
     5581 cgcagggtcg cggaaaaact gcaatgcaaa tacggtaaac acccaaaacg gcaaagacca
     5641 ccagccgcag cacatcgaca ccagcaggct caaagccaaa ccgccgccga taatcggcca
     5701 gccctcacgg gcgataatcg ggtggggata aagacggttc atttgtcatc cttttattca
     5761 aatatattaa atagcgacat tataacggaa tccgccgcgt tccgccgaca aatgccgtct
     5821 gaacgcggct tcagacggca tccgagaaag tgctacaata tcggttttcc tttatttttt
     5881 caggcggcat ccattatgtg caaccatcat ccccgacatt cgcacgacaa cgacaccatc
     5941 cgcattcgcg gcgcgcgcac gcataatttg aaaaacatcg atttggacat tccccgccac
     6001 aagctcgtgg tggtaacagg attgtcaggc agcggcaaat catcgctggc gtttgatact
     6061 ttatatgccg aagggcagcg gcgttatgtc gagagcctgt ctgcctatgc gcggcagttt
     6121 ttgcagatga tggacaaacc cgatgtcgat ttgattgaag gcctgtcgcc tgcgatttcc
     6181 atcgagcaga aatccaccag ccacaatccg cgttccaccg tcggcacggt aacggaaatc
     6241 cacgactacc tgcgcctttt atacgcccgt gtcggcacgc cgtattgccc cgaacacaag
     6301 ctgccgctat ccagccaaac cgtatctcaa atggtcgatg ccgtattaaa actgccggaa
     6361 gatacgcgcg tgatgattct ggggccggcg gtgcgcgagc gtaagggcga gtttgtcgat
     6421 ttctttgccg acttgcaggc gcaaggcttt gcccgtgtgc gcgtggacgg cgaagtctat
     6481 cagcttgacg aagtaccgaa gctggaaaaa aacatcaaac acaatatcga cgtcgtcatc
     6541 gaccgcgtga aagtgaaagc cgacatcaaa caacgactgg cggaaagttt tgaaaccgcg
     6601 ctgcgccacg gcaacgagcg cgcgctggcg atggaaatgg acagcggcga agaacattgg
     6661 ttttccgcgc gttttgcctg ccccgtatgc tcatacagcc tgcccgaatt ggaaccgcgc
     6721 ctcttttcgt tcaacaaccc gatgggttcc tgcccgactt gcgacggctt gggcaacacc
     6781 aatttcttcg accccgaaaa agtagtcgcc catcccgaat tgtcgctggc aaccggcgcg
     6841 attgacggtt gggacaaacg caaccaattc tatttccaaa tgattcaatc gctggcgcat
     6901 cattataaat ttgatgtcaa cgtcgcttgg gaaactttgc ctgaaaaagt caaaaaagtc
     6961 gtgttgcacg gctcgggcaa agaagtcatt gatttcacct acctgtccga acgcggcacc
     7021 accttcaacc gcagccacgc cttcgagggc atcatcccca atctcgaacg ccgctaccgc
     7081 gaaaccgaca gcgaaaccgt gcgcgaaaaa ctgcgcgagt accaaaatca ccgtgcctgc
     7141 ccgagctgcg gcggcgcacg tttgcgcaaa gaagcacgct acgtttacgt cggcggcgaa
     7201 ccgttgcacg aagtctccgc ctggccgctg accaaaaccc accgattctt cgaaacgctg
     7261 gatttggacg gcaacaaaaa acagatcgcc gaaaaaatcc tcaaagaaat caccgagcgg
     7321 ctcggcttct tgattaacgt cgggctggat tacctgaatc taagccgttc cgccgaaacc
     7381 ctttccggcg gcgaagccca gcgcatccgc cttgccagcc aaatcggcag cggcctgacc
     7441 ggcgtgatgt acgttttgga cgaaccctcc atcggcctgc accagcgcga caacgaccgc
     7501 ctgctcgcca ccctcaaacg cctgcgcgat ttgggcaaca gcgtaattgt ggtcgaacac
     7561 gacgaagacg ccatccgcga agccgatttc gtcgtcgata tgggccccgg cgcgggcgaa
     7621 cacggcggca acgtactgat tgccgacacg cccgaaaacg tcgccaaatg cgaaaaatcc
     7681 gttaccggac aatacctcgg cggtaaaaaa tccattgccg tgccgtctga acgcacgccc
     7741 gtcaatcccg gccgaatgct cgtcctcaaa ggcgcgcgcg gcaacaacct caaaaacgta
     7801 accctcgagt tgccgcttgg tttgattacc tgcatcaccg gcgtatccgg cagcggcaaa
     7861 tccaccctga ttaacgacac cctcgccaaa atcaccgccc gcgaactcaa ccgcgcccaa
     7921 gaagaacccg ccccatacga cgacatccgc ggcctcgaac acctcgacaa agtcatcaac
     7981 gtcgaccaat cccccatcgg ccgcaccccg cgctccaacc ccgccaccta caccggcctg
     8041 ttcaccccca tccgcgaact cttcgccggc gtacccctct cgcgcgaacg cggctacaac
     8101 gtcggcagat tctccttcaa cgtcaaaggc ggccgctgcg aagcctgcca aggcgacggc
     8161 gtaatcaaag tcgaaatgca cttcctgccc gacgtgtacg tcccctgcga agtctgccac
     8221 ggcaaacgct acaaccgcga aaccctcgaa atccaataca aaggcaaaaa catcagccaa
     8281 gtcctcgaca tgacggtcga agaagcccgc gaatttttcg acgccgtccc caccgtatcg
     8341 cgcaaactgc aaaccctgat ggacgtaggc ctaggctaca tccgcctcgg ccaatccgcc
     8401 accaccctct ccggcggcga agcccagcgc gtcaaactcg ccttggaact ctccaaacgc
     8461 gacaccggca gaacgctcta catcctcgac gaacccacca ccggcctgca cttcgccgac
     8521 atcgccctgc tgctggaagt cataggccgt ctgaaaggca aaggcaactc gattgtgatt
     8581 atcgagcata atctggatgt aattaaaacc gcggattgga ttgtggactt agggccggaa
     8641 ggcggcgatg gtgggggtaa ggttatcgcg aaaggtagcc ctgaacaagt ggctaaaatt
     8701 aaggggagtt ataccgggaa gtatttgaaa gtggttttga agtaaacgaa cggatataga
     8761 aaatgaacaa taattttgac cgcagcaagc atatctatca taacgaagct tttgtagaac
     8821 tggttaaaga tgccatacgc ttttttcatg gtacgcctgt tcatacactt cctccaccca
     8881 aacagttcaa aggtgcaggg gtttatgcga tttactatat cggtaacaat cctttgtaca
     8941 aacaatatgc ggactggaac agattatcct acaatgcgcc aatttatgtt ggcaaggccg
     9001 tccccaaagg ctggcggcaa gcaagaaatt ctgataatgc gctaaaccaa tcaaccgaac
     9061 tgttccaccg gttgaaagaa cacagcagga gtattgccgc tgtttctgat ttagatcctt
     9121 ccgatttcat gtgcagattc gttatctttg aaggtgccgg ttctaacatg ataggcacaa
     9181 tcgaagctgc cctgattaaa ttacacaaac ctctatggaa ctcttgtgtc gatggtttcg
     9241 gaaaccacga ccccggaaaa ggccgttacg aacaagccaa atcggattgg gatgtccttc
     9301 atagcgggcg ggtttgggca gataggctaa acggcatacc gaatagctat gagtccatac
     9361 ttgaaaatat caacacacat ttagagataa taaagagaaa ataacctatg aagtctttgg
     9421 aaattttttc cggcgcgggc gggttggcaa aaggccttga acttgcagga ttccaacatg
     9481 catcttttat tgaactcaat aaagatgcat gcaattcttt aagaagcaac tttaacccca
     9541 agcttgttta tcagggagat gtggcagatt ttgatctttc cagtcaagaa ggcatagaag
     9601 tcattgcagg cggtcctcct tgccagccct tttcattggg cgggaaacac cttgcccatg
     9661 aagacagaag ggatatgttt cctcatgcgg tcagatatgt ggagtattac cgccccaaag
     9721 cttttatctt tgagaatgta aaaggacttt taagaaaatc attcgctgat tattttgaat
     9781 atatcctact tcggcttact tatccgaatc ttggtatttt gcagaatgaa gattggaaag
     9841 gccatctgac acggctgaaa gaaattgaat tcaatttata taaaggaatc aaatataagg
     9901 tttcatatca attgttgaat gcagctgatt acggagtgcc gcaaaaacgc gaacgcgtgg
     9961 ttatcgtagg tatccgggcc gatttggata ttgattggaa attcccaaaa agaacacatt
    10021 ccgaagacag gttgaattgg gaaaagtatg taacggggga atactgggaa aaacacaacg
    10081 aacccaaaag attcaataaa gatattgctg aaaagttaca aaaaaaatac ggtatattcg
    10141 aaccagaaaa aaaaccttgg caaacggtaa gggatacctt gtccgacatc ccgcatcctt
    10201 tggggaatca taaaattaca ggacatgaat atagggatgg cgcaagaatt tatcccggac
    10261 acacaggaag cgggatagac gaaccgtcca aaaccattaa agcaggtggg catggcgttc
    10321 ccggcggaga aaatatgatt cgttatgatg atggaacagt cagatacttt accagctatg
    10381 aagcaaaact tcttcaaaca ttccctgaag aatttgtcat ttctggagct tggggagaag
    10441 caatgcgaca aattggcaat gcggttcctg tcaaattgtc ggaaatttta ggcaaacatc
    10501 tgatgggggt gttgtcggag aaaagcagcc tgcacaacta acccgcccac tgcgtgtgtg
    10561 cggtacgcac gcggttgctt gattgacggt ttgccgtttc aacttttaac ttcgttgaag
    10621 ctagtgcttt cagacggcct ccaatgtcgt ttggaaacat caggtccaac aaagttaaac
    10681 cttaaacaac aaaccattcc ttctccaaca cccaaaccac aaacaaaaat cctatggcgc
    10741 gttatcgcag aaacttcatt gccggcggca cattcttttt caccgtcaaa cccgccgccc
    10801 cgaaatcgcg tctgcttgtc ggacatatcg gcttcgcgtg cggcttatat ggatgtgcaa
    10861 aaacaatatc cctttgaaac cgtcgccgta tgcgtgctgc cgaaccatat tcacgccatt
    10921 tggacgctgc cgcccgacga tgcggattat tccctactcc ggcggctgat taaaaccaaa
    10981 ttctccgcat attcccctca tactaaaaaa
//
LOCUS       DAWTQS010000064        10811 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.064, whole genome shotgun sequence.
ACCESSION   DAWTQS010000064 DAWTQS010000000
VERSION     DAWTQS010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10811
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.064"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(<1..188)
                     /locus_tag="WNA25_001722"
     CDS             complement(<1..188)
                     /locus_tag="WNA25_001722"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824429.1"
                     /translation="MFPPAEITGRGRVVLQQGFAGFGLYFHGVKFRRLNIPFKLPASC
                     RAVKYARMFAAVSADFGG"
     gene            313..996
                     /locus_tag="WNA25_001723"
     CDS             313..996
                     /locus_tag="WNA25_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HFA9824430.1"
                     /translation="MDKEKVLDKIKKCLALGRSANEHEAAQALRQAQALMEKYKVNAE
                     DIALSKVSEQKADRKMAFKLAGWQWGVANMIADIFGCKSYQRGKTMMFYGIGNRAETS
                     AYAFDVVYRQISADRRKFLKTCRAGKPSHRTYLADRFCGGWIASAWETVKKFEMSDEE
                     KAIMDGYKKKEYPDMAEARTRDAKSSILQGSKMEYEALTRGMESGKQVKLHYAVNGTG
                     FVKQIGGQT"
     gene            1191..1460
                     /locus_tag="WNA25_001724"
     CDS             1191..1460
                     /locus_tag="WNA25_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824431.1"
                     /translation="MESSDVQGRMETLAFATAPKKGGIRKSGFGRVILIKSGHIVIHN
                     ADGDSFATGYGVIAADIVSPDLKTDFDIFFPPESLLRYKKIGKKK"
     gene            complement(1816..2172)
                     /locus_tag="WNA25_001725"
     CDS             complement(1816..2172)
                     /locus_tag="WNA25_001725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824432.1"
                     /translation="MPLSRAMRWVLDQARGLNVNGRFVFESVNFQGHINKESPRVAMR
                     RSGLDTTAHGLRSLARTYLREVLKVYNDVAEKLLAHSLGTRTQTAYNRSELWEERKDA
                     PERWGNDVLRLADNGK"
     gene            complement(2121..3016)
                     /locus_tag="WNA25_001726"
                     /pseudo
     CDS             complement(2121..3016)
                     /locus_tag="WNA25_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259344.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
     gene            complement(3207..3296)
                     /locus_tag="WNA25_001727"
     tRNA            complement(3207..3296)
                     /locus_tag="WNA25_001727"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3260..3262),aa:Ser,seq:cga)
     gene            complement(3373..3463)
                     /locus_tag="WNA25_001728"
     tRNA            complement(3373..3463)
                     /locus_tag="WNA25_001728"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3427..3429),aa:Ser,seq:tga)
     gene            complement(3546..4325)
                     /gene="yaaA"
                     /locus_tag="WNA25_001729"
     CDS             complement(3546..4325)
                     /gene="yaaA"
                     /locus_tag="WNA25_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFA9824433.1"
                     /translation="MFFVLSPAKNLNEKDPCPVSEFTQPDLLAESEILMRQLRELAPQ
                     QIAELMHVSDKIALLNAERNAAWHTPFTPENAKQAVFMFNGDVYEGMDANTLNTNQIQ
                     YLQNHVRLLSGLYGLMRPLDLMQPYRLEMGTAFANLRGKNLYEFWGDIITNLLNDTLA
                     QAGSNTLVNLASQEYFKSVNTKKLRARLITPIFKDEKNGKYKIISFYAKRARGLMVRY
                     AAEHNITDPEMLKNFNYEGYAFNDAASNESEWVFMRSEQIK"
     gene            complement(4481..5668)
                     /gene="dapC"
                     /locus_tag="WNA25_001730"
     CDS             complement(4481..5668)
                     /gene="dapC"
                     /locus_tag="WNA25_001730"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HFA9824434.1"
                     /translation="MNTLLKQLKPYPFARLREAMQGISAPEGMEAVPLHIGEPKHPTP
                     KVITDALTASLRELEKYPLTAGLPELRQACANWLKRRYDGLTVNPDNEILPVLGSREA
                     LFSFVQTVLNPVSDGLKPVIVSPNPFYQIYEGATLLGGGEIHFANCPAPSFNPDWRSI
                     SEEVWERTKLVFVCSPNNPSGSVPDLDGWREVFDLQDKYGFIIASDECYSEIYFDGNK
                     PLGCLQAAAQSGRSRQKLLMFTSLSKRSNVPGLRSGFVAGDAELLKNFLLYRTYHGSA
                     MSIPVQRASIAAWNDEQHVIDNRRLYQEKFERVIPILQQVFDVKLPDASFYIWLKVPD
                     GDDLAFARNLWQKAAIQVLPGRFLARDTEQGNPGEGYVRIALVADVATCVKAAETIVC
                     LYR"
     gene            complement(5740..6192)
                     /gene="dut"
                     /locus_tag="WNA25_001731"
     CDS             complement(5740..6192)
                     /gene="dut"
                     /locus_tag="WNA25_001731"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFA9824435.1"
                     /translation="MNIEVEMKVLDERMADFIPAYATEGSAGLDLRACLDEEVVLQPG
                     ETFLVPTGLAIYLANPAYTAVLLPRSGLGHKHGIVLGNLVGLIDSDYQGELKVSLWNR
                     GSEPFTVKPFERIAQMVIVPVVQAGFKRVEEFVGSSRGEGGFGSTGSH"
     gene            6358..7069
                     /locus_tag="WNA25_001732"
                     /pseudo
     CDS             6358..7069
                     /locus_tag="WNA25_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686491.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
     gene            7066..7374
                     /locus_tag="WNA25_001733"
     CDS             7066..7374
                     /locus_tag="WNA25_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD family protein"
                     /protein_id="HFA9824436.1"
                     /translation="MKDLLSFPSFLLFSCMLAVTCPTQLIGFFALRNRTLSRRAQTVM
                     ETAPGCVLISVIAPYFVSDKPHELIAIALTAFAACRFSMLFTVLIGVGSSGISGWLMA
                     "
     gene            complement(7444..7917)
                     /locus_tag="WNA25_001734"
     CDS             complement(7444..7917)
                     /locus_tag="WNA25_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFA9824437.1"
                     /translation="MEQKGFTLIEMMIVVAILGIISVIAIPSYQSYIEKGYQSQLYTE
                     MVGINNVLKQFILKNPQDNNQIIKSKLETFVLGYKMNPKIAKKYSVSVAFANTEKPRA
                     YRLVGVPKAGTGYTLSVWMNSVGDGYKCRNATSAQTYSETLSANTGCEAFSNRKK"
     gene            complement(7919..8530)
                     /locus_tag="WNA25_001735"
     CDS             complement(7919..8530)
                     /locus_tag="WNA25_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HFA9824438.1"
                     /translation="MRKQNTLTGIPTSDGQRGSALFIVLMVMIVVAFLVVTAAQSYNT
                     EQRISANESDRKLALSLAEAALREGEFQVLDLEYAADSKVTFSENCEKGLCTAVNVRT
                     NDNGNEEAFGNIVVQGKPTVEAVKRSCPAKSGKNSTGLCIDNQGVEYKKGTGKVSKMP
                     RYIIEYLGVKNGQNVYRVTAKAWGKNANTVVVLQSYVGNNDEQ"
     gene            complement(8509..9450)
                     /locus_tag="WNA25_001736"
     CDS             complement(8509..9450)
                     /locus_tag="WNA25_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HFA9824439.1"
                     /translation="MKRKMLNVPKGGYDGMKGFTIVEFLVAGLLSVIVLIAVVSSYFT
                     SRKLNDAANERLAEQQDLRNAATLIVRDARMAGSFGCFNMSEHIGNDVVFNVAQKNAL
                     FSLKRNSTNSTNKLIPITESPNINYQNFFQVSSALIFQYGIDDVDASADTTVVSSCAA
                     ISKPGKQIPTLEDAKKELKIQNSDKEQNGNIARQRHVVNAYAVGKIAGEEGLFRFQLN
                     EKGEWGNPQLLAKKIKRMRVRYIYVSGCPEDEDAGKEEQFKYTDKFDSSVTPAGVEVL
                     LDSGSDAKIAASSDNIIYAYRINATIRGGNVCANRTL"
     gene            complement(9447..10055)
                     /gene="pilV"
                     /locus_tag="WNA25_001737"
     CDS             complement(9447..10055)
                     /gene="pilV"
                     /locus_tag="WNA25_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HFA9824440.1"
                     /translation="MKNNDCLRLKNPQSGMALIEVLVAMLVLTIGILALLSVQLRTVA
                     SVREAETQTIVSQITQNLMEGMLMNPTIDSDSNKKNYNLYTGSYPPTSSDGDFKLNNL
                     ISKTDLAKAQLDRFGYELKQALPDAVAIRYAVCKDSSGDAPTLSGNTFSSNCDKKANG
                     DTLIKVLWVNDSAGDSDIFRTNLGVSGGNIVYTYQARVGGRE"
     gene            complement(10087..10749)
                     /locus_tag="WNA25_001738"
     CDS             complement(10087..10749)
                     /locus_tag="WNA25_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tfp pilus assembly protein FimT/FimU"
                     /protein_id="HFA9824441.1"
                     /translation="MCTRKQQGFTLIELLIVMAIAAIMATIALPNMGGWIASRRIASH
                     AEQVANLLRFSRGEAVRLNLPVYICPVQVKKDGAPNNGCDFSKKGRGMLAFGDKNGNK
                     AYDGDTKDVFLRNVVLNDTDDSRINYAFNHIAFGSSQPKADRVVWTFNQNGTFGYLPD
                     QNLKDNSKFVYSDGYIQIVLTDARAVSDADKKFRSAVVLIDSSGRVEVCRKNDTRAVC
                     KH"
BASE COUNT         2857 a         2821 c         2416 g         2717 t
ORIGIN      
        1 ttgccgccaa agtccgccga tactgcggcg aacatccggg cgtatttgac ggcgcggcag
       61 gaagcaggca gcttgaacgg tatattaagc cgtctgaatt tcacgccgtg gaaatacagc
      121 ccgaagcctg caaagccttg ttgcaaaact accctgccgc ggccggtaat ctcggcaggc
      181 ggaaacaaaa aaagccccga gttcgtaagc gtatcagccc tagccgaatg gcccgaccga
      241 atcgaaagca aagcgaaaga cgaatgggca agggtgcgaa attaaaatac cggacaaatt
      301 aaagggttta atatggataa agagaaagtc ttagacaaaa tcaagaaatg tttggctttg
      361 ggtcggtcgg caaatgaaca tgaagccgca caggcgttaa ggcaggcgca ggctttaatg
      421 gaaaagtaca aagtaaatgc cgaggatatc gccttatcaa aagtctccga acagaaagcc
      481 gatcggaaaa tggcttttaa attggcaggc tggcaatggg gcgttgcaaa tatgattgcc
      541 gatatattcg ggtgcaaatc ttaccaacgc gggaaaacaa tgatgtttta cggcattgga
      601 aatcgagcgg aaacctcagc ctatgccttt gatgtagtct atcgtcagat ttccgccgac
      661 cgccgcaaat ttttgaaaac ctgccgggcg ggaaagccct cgcacagaac ttatcttgcc
      721 gaccgatttt gcggcggttg gattgcgagc gcttgggaaa cagtcaaaaa atttgagatg
      781 tcagatgaag aaaaagccat tatggacgga tacaaaaaga aagaatatcc ggatatggcc
      841 gaagcaagga caagagatgc gaaatcgtca atcctacaag ggtcaaaaat ggaatatgag
      901 gcattaaccc gaggaatgga atcgggtaag caagtgaagt tacactatgc cgtgaacggt
      961 acgggcttcg taaaacaaat cggagggcaa acatgaatcg atacggcggc ggatgcggaa
     1021 acttcggcgg attggggaaa ggatatgccg aatatgaaag acgcaattgc cgcatgccaa
     1081 gccgtaaaaa agcggaaacc cggacaaatc ggaatcaacg gaaagaggat tgaagacatg
     1141 gaatacgaag tattcaataa acgcccgtca aaaaagcggt cggcgaagca atggagtctt
     1201 ccgacgtaca agggcggatg gaaacactgg catttgcaac cgccccgaaa aaaggcggaa
     1261 tccggaaatc cggattcggc cgcgttattc taatcaaaag cgggcatata gttattcata
     1321 acgctgatgg cgacagcttt gccaccggct acggcgtgat tgccgccgac atcgtcagtc
     1381 ccgatttgaa aacagacttt gacatattct tcccgccgga aagcttgttg cgatataaaa
     1441 aaatcgggaa gaagaaatag gcagacaaac gacagagccg taaaaacggc gcaagattaa
     1501 accgaacagg agagtaaaaa aatgaacatc accaaaccgg gcgcaaggat agaccgcccg
     1561 accatccgcg aactgattgc ctacgcaacc tgccgcaacc accccatttc aaactcgaca
     1621 ctgctgcgta tggaaaaaga cggacggata ccgtgccgtc tgaacctccc tcgcatcccc
     1681 cgtatgggac acccgcgaag ttttggaagc attagggttg cagcaataaa aaaacgcccg
     1741 attagggcgt tttcacataa tgcggaaaca gctttcttat ctacatttcc acataacgca
     1801 aaacacacaa aaaaatcatt ttccgttgtc ggcaagcctc aaaacatcat tcccccaccg
     1861 ttccggcgca tccttacgct cctcccaaag ctccgagcgg ttgtaagccg tttgcgtcct
     1921 cgtccccaac gaatgggcaa gcagcttttc cgccacatca ttatacacct tcagaacctc
     1981 gcgcaaataa gtacgcgcaa gcgagcgcaa accgtgcgcc gtcgtatcca gccccgaccg
     2041 ccgcatcgcc acgcgcggac tttccttatt gatatgccct tgaaaattca cactttcaaa
     2101 cacaaaccgc ccgttcacat tcagcccccg cgcctgatcc aacacccacc gcatcgcccg
     2161 cgacagcggc acatcatgcc cccgccgctc ctttttccgt tcggcgggga tacgccaaac
     2221 ctccccgtcc aactccgacc actccatcaa cgccgcctcc cgaatccgcg tcatcgtcaa
     2281 cagcagccaa tagatacaaa accgcgttac aggatggata tgcaccctgc ccgcataaac
     2341 cgcaaattca ttgcgctgct ccaaaaaatc aatcaggcgc ggcaattcgg acggactcaa
     2401 cgccgtcatg tttttggttt tcacccgttc aaacacaccc gaaccgattt gcgcgacagg
     2461 gttgatttcc atcattcccg aaccgaccgc aaacgcaaac gcaaacacca tcttcagact
     2521 gtttttcgtt ttgcgcaacg tatcaacgat accgcgcgcc tccatcacac gcaggcagcc
     2581 gaccacgtcc gccgtcctga cttgacggat atcaagattg ccgatagccg gaaaaaccca
     2641 ccgttcaaaa ttccgcataa cctgtccggc atacttttca gaccgcccct tcgaccaacg
     2701 cacaaaccaa tcacgcgcca ccttctcaaa agcaaaatcc gcccgcacct tcttattgac
     2761 gacattttcc ccgtgtgccc gttttcggcg cacctcctcc cgccattccc gcgcctcagc
     2821 cagcgagaaa tcaggatacc gccccagcga aattgtctgt tgccttccgt cctgcataaa
     2881 cgacagcttc caactccgtc cgcccgtcgg gtagacccac aaagccagcc cccccgtccg
     2941 acaacttata cagcttatcg cgcggcttcg catttttaac ctgatttgcc gacagcggcg
     3001 taatgatttt cgccattatg gtaaattccc gtttgtccaa aatttaccat ataaattacc
     3061 ataaaaaaat gcaataagaa gatgccgcaa gattgcaaat gatttgcctt gaagcgttat
     3121 gataggtcaa gccgatgatt tttatatggt aatcaacaaa acttgaacaa aaaagaaccg
     3181 ccctgaatca gggcggttat gttttctggc ggaaacggtg ggattcgaac ccacggagga
     3241 tttgcaccct cagcggattt cgagtccgct gcattcagcc tctctgccac gtttccgata
     3301 atgcagtaaa accaaataaa aataacaata ttcgcggcta ttgcattttt atttggtttt
     3361 acgctcaaaa attggcggaa gcggtgagat tcgaactcac ggagggctat caaccctcga
     3421 cggttttcaa gaccgttgca ttaaaccgct ctgccacgct tccgtcttct tgaagagtag
     3481 gaataataat gaagtttata cattttgcca agcacttttt ttgggaaata cttaatttat
     3541 tgttttcact ttatttgttc cgaacgcata aacacccatt cgctttcatt ggaagccgcg
     3601 tcattgaatg cgtagccttc gtaattaaaa tttttcagca tttcaggatc ggtaatattg
     3661 tgttctgccg catagcgcac catcaatccg cgcgcgcgtt tggcatagaa actgatgatt
     3721 ttatatttac cgtttttttc gtctttaaat atcggggtaa tcagccgcgc ccgaagtttt
     3781 ttcgtgttga cggacttgaa atattcctgt gaggcaaggt tgacaagcgt attgctgcct
     3841 gcttgggcaa gcgtgtcatt taaaaggttg gtaatgatgt cgccccaaaa ctcatacaaa
     3901 ttcttgccgc gcaaattggc aaatgccgtt cccatttcca ggcggtaggg ctgcatcaaa
     3961 tccagtggac gcataagacc gtacaagccg gacagaaggc ggacatggtt ttgcagatat
     4021 tgtatttgat tggtattcaa tgtgtttgca tccatacctt cgtaaacatc gccgttgaac
     4081 ataaagactg cctgtttggc gttttccggc gtaaacggcg tgtgccacgc tgcatttcgt
     4141 tcggcgttta gaagtgcgat tttgtcggaa acgtgcatca actctgcaat ttgttgaggg
     4201 gcaagctccc tcagttggcg catcaggatt tcagattccg ccaaaagatc gggttgggta
     4261 aactcgctga cagggcaagg gtctttttcg ttaaggtttt tcgcagggga aaggacaaaa
     4321 aacataatcc gctcggtata gtgtcaaacg tcggaatttt agatttaaaa gcatcctcag
     4381 gcaagccgat tgattgtgat gtaaataccg ttataataaa tacatcttga tagtattaaa
     4441 aaaagccgtc aggaaaagtc cggacggcat tttttattct ttaccgatat aagcaaacaa
     4501 tggtttccgc agctttgaca caagttgcga catctgcaac caaagcaatc cgcacataac
     4561 cttccccggg attgccctgt tcggtatccc gtgccaaaaa gcgtccgggc aatacttgga
     4621 tagcggcttt ttgccataaa ttgcgtgcga atgccaaatc gtcgccatcg gggactttca
     4681 accagatgta aaacgaggca tccggtaatt taacgtcaaa tacctgttgc aaaatgggaa
     4741 taacacgctc aaatttttcc tgatacaggc ggcggttgtc gataacgtgt tgttcgtcat
     4801 tccaggcggc aatactggca cgctgcacgg gaatgctcat tgcgctgccg tgataggttc
     4861 tgtaaagcag aaagttctta agcagttccg catcaccggc gacaaaaccg gaacgcaggc
     4921 ccggaacatt ggaccgcttg gacaagctgg taaacataag cagtttttgc ctgcttcgcc
     4981 ccgactgtgc ggcggcctgc aggcagccca aaggtttgtt gccgtcaaaa tagatttcgg
     5041 aatagcattc atccgaggca ataatgaaac catatttatc ctgtaaatca aaaacttctc
     5101 tccatccgtc caaatccggc acgctgccgc tggggttgtt gggcgagcag acgaacacca
     5161 gtttggtgcg ttcccaaacc tcttcggaaa tactgcgcca atcggggttg aaagacggcg
     5221 cggggcaatt ggcaaaatgg atttcaccgc cgcccaaaag tgtcgcacct tcataaatct
     5281 gataaaaggg attcgggctg acgattacgg gttttaggcc gtctgaaaca gggttcaaca
     5341 cggtttgaac aaaagaaaac aacgcctccc tactgcccaa aaccggcaga atttcattat
     5401 ccggattgac cgtcaagccg tcataacggc gttttaacca gttcgcacac gcctgacgca
     5461 gttcaggcag acccgcagtc agcggatatt tttccaactc gcgcaatgag gcggtcagcg
     5521 catccgtaat gactttcggt gtcgggtgtt tcggttcgcc gatatgcagt gggacggctt
     5581 ccatgccttc aggcgcggaa atgccctgca tcgcctcgcg cagtcgggca aagggatatg
     5641 gtttgagctg cttgagtaag gtattcatga ttttctccgg ttatcggtat atcggaagat
     5701 atgccgcctg aaccgtcctt cagacggcat tctatatctt tagtgagaac ccgtactgcc
     5761 gaagccgccc tcaccccggc tgcttccgac aaattcctcg acacgtttga agcccgcctg
     5821 tacgaccggc acgataacca tttgtgcgat acgctcgaac ggtttgacag taaacggttc
     5881 gctgccccta ttccataacg acaccttcaa ttctccctga taatcggagt cgattaagcc
     5941 gaccaaattg cccaagacaa tgccgtgttt atgccccaag ccggaacggg gcagcaaaac
     6001 ggcggtatat gcgggattcg ccaaataaat tgccaaacct gtcggcacaa gaaacgtttc
     6061 gcccggctgc aaaacgactt cctcatccaa acaggcgcgc aaatccaaac ctgcagaacc
     6121 ctccgtcgca taggcaggga taaaatccgc catcctctcg tccaacactt tcatttctac
     6181 ttcaatattc atcgtcctgc tcctgcatac ggccggtttc aaagattgga gattatacac
     6241 aagccgtgtt tacgctgaaa cacaacaaac tgcttttgtt ttgaaaccat cctcgactgt
     6301 caaaaacatt ctgcctgtgt atgataccgc cccgtctctt catatataaa cattaacatg
     6361 acgcacacag cctctccacg tgatgaattc atacgcggca tcaaagaaag ttcgcccatg
     6421 ctgattgggc ttttgccttg ggcattgata ctcggaatgc agggcgggca aaaaggtatg
     6481 ggccggctgg aaatgctgct gatgacgggg atgaacttcg ccggcggctc cgaatttgcc
     6541 acggtcaacc tgtgggcgga acctctgccg atactgccta tcgccaccat aacctttatg
     6601 attaattcgc ggcatatcct gatggggggc ggcgcttgcc acgcacatga aagaaatacc
     6661 gctgaaaaaa gccgtgcccg cgctgctttt tatgtgtgat gaaagccggg ccatggcatt
     6721 ctccgaaatc caaaaacgga aagcagccgg tttgcctgca ttcaatatgc ctttttatat
     6781 gggcgcatgc tttatcctct acattacctg gatagggttt gccgccctcg gcgcggcagt
     6841 agggcccgtg ttcggtaatg tggccgcttg ggggttcggt atggcgtttc ctgccgtatt
     6901 tttggtttta ctgaggggaa tgtggaaaag tttttccgca tcaagacttt ggtttgtcaa
     6961 cctgtttgtc gcatccgccg tttacctgac cgttgacggc gcgtggtatg ttcccgccgg
     7021 cacgctgtcc ggcctgttgt ccgcctattt ttggaatggg cggaaatgaa agatttattg
     7081 tcgttcccat cattcctgct gttttcctgt atgcttgccg ttacctgccc tacccagtta
     7141 atcggctttt tcgcattgcg taaccgaacc ttaagccgcc gcgcccaaac cgttatggaa
     7201 accgcgccgg gctgcgtcct catttccgtt attgccccct attttgtctc cgacaagccg
     7261 cacgaactta ttgccatcgc gttaaccgcc tttgcggcct gccgtttttc tatgttgttt
     7321 accgtattga taggggtcgg ctcgtcaggc atctcgggat ggttgatggc ataattcatt
     7381 tcaaaatgcc cataaaaaat accgtctgaa aattgatctt cagacggtat tggcaaaaca
     7441 accctatttt ttacgattag agaaagcttc acagccggta tttgcggaca aggtctccga
     7501 ataggtctgg gcagaagtgg cattacggca tttgtatccg tcgcccacgc tgttcatcca
     7561 taccgacaag gtataacccg tccctgcctt cggaacgccg accaacctgt atgcccttgg
     7621 tttttccgta ttggcaaatg ccaccgaaac actatatttt ttggcaattt tcggattcat
     7681 cttatagcct aagacaaatg tttccagttt gctcttgatg atctgattat tgtcctgggg
     7741 atttttcaaa ataaactgtt tgagaacatt gttgataccg accatctccg tataaagctg
     7801 ggactgatag cctttttcaa tataactttg ataagaaggt atggcaatga cgctgataat
     7861 gccgagtatc gcgacaacta tcatcatctc aatcaatgta aacccttttt gttccatttt
     7921 attgctcatc attattgcct acataagatt gaaggacgac cacggtattg gcattcttac
     7981 cccaagcctt ggcagtaacc cgataaacat tttgtccgtt cttcacgcct aaatattcga
     8041 taatatagcg cggcattttg ctgacttttc ccgtaccttt cttatattcc actccctgat
     8101 tgtcaatgca caggccggta gaatttttgc cagactttgc agggcaagaa cgcttcaccg
     8161 cctcaacggt gggcttgcct tgcaccacga tattgccaaa agcctcttca ttaccattat
     8221 catttgtccg cacattcact gcggtacaca gacctttttc acagttttcg ctaaacgtaa
     8281 ccttactgtc cgcagcatat tccaaatcca aaacctgaaa ttcgccctcc cgcaaagccg
     8341 cctcggctaa agacaaagcc aatttcctgt ctgattcgtt ggcactgatc ctctgttcgg
     8401 tattgtagga ctgggcggca gttacaacca aaaaggccac gactatcatc accatcagca
     8461 cgataaacag tgcggacccc ctctgtccgt cagaagtcgg gattcctgtc aaagtgttct
     8521 gtttgcgcat acatttcccc cgcgtattgt cgcattgata cggtaagcat aaataatatt
     8581 atctgaagaa gcggcaatct tggcatcact accgctatcc aataaaacct ccaccccggc
     8641 aggcgtaaca gagctgtcga atttatccgt atatttgaat tgttcctctt tgccggcatc
     8701 ttcatcttca ggacaaccgg aaacatagat ataccgcact ctcatacgtt taatcttttt
     8761 cgcgagcaac tgaggattac cccactcgcc cttttcattc aattggaagc ggaacaaacc
     8821 ttcctcaccg gcaatcttgc cgaccgcata ggcattgacc acatgccttt gacgcgctat
     8881 attgccattt tgctccttat cagaattctg aatcttcaat tctttttttg catcttctaa
     8941 agtagggatt tgcttacccg gtttcgatat tgcggcacag ctgctgacga cggtagtatc
     9001 ggcgcttgca tcaacatcat cgattccgta ttgaaaaatc aatgcgctac taacctggaa
     9061 aaaattctga taattgatat ttggagattc cgttatgggg ataagtttat ttgtactgtt
     9121 tgtactgttc ctttttaagg aaaaaagagc gtttttttgc gccacattga aaacaacatc
     9181 atttccgata tgctcagaca tattgaaaca accgaagctc cccgccattc ttgcatcgcg
     9241 gacaatcaat gttgccgcat tccgcaaatc ctgttgctcg gcaagacgct cgtttgccgc
     9301 atcatttaat ttccgggatg taaagtaact cgatacgacc gctatcagga caatcacact
     9361 gagcaggccc gcaaccagaa attcaacaat ggtaaaaccc ttcataccat cataaccgcc
     9421 ttttggtacg tttagcattt tacgtttcat tcacgacctc cgacccttgc ctgataggta
     9481 tatacgatat tgccgccgct caccccaaga ttcgtacgga aaatatccga atcccctgcc
     9541 gaatcattta cccacaatac tttaatcaaa gtatccccgt ttgccttctt gtcgcaattt
     9601 gaagaaaaag tattgccgga caatgtcggc gcgtcacccg acgaatcctt gcagacggcg
     9661 taacgaatag ctaccgcatc cggcaaggct tgtttcaatt cataaccgaa cctgtccaac
     9721 tgggcttttg ccaaatccgt cttacttatc aaattattaa gcttgaaatc gccgtcagag
     9781 gaagtggggg ggtacgaccc cgtgtaaaga ttatagtttt tcttgttgct gtccgaatca
     9841 atggtcggat tcatcaacat tccttccatc aggttttgcg tgatttggct gacgatggtt
     9901 tgtgtctccg cctccctgac ggaagcgact gtccgcaact gtacggacag caatgccaaa
     9961 ataccgatgg tcagaacgag catagcgacc aagacttcta tcaacgccat accggactgg
    10021 ggatttttca ggcgcaagca atcattattc ttcatttttt atttcccaaa ccgactaaaa
    10081 cagtcattaa tgtttgcata cggcgcgcgt atcgttttta cgacaaactt cgaccctgcc
    10141 gctgctgtca atcaaaacca ccgccgaacg gaatttctta tcggcatctg aaactgctct
    10201 cgcatctgtc agcacgattt ggatataacc gtcggaataa acaaatttgg aattatcctt
    10261 gagattctga tcgggcaaat agccgaatgt cccgttttgg ttgaacgtcc aaactacacg
    10321 gtcggctttc ggctgagacg aaccgaaagc gatatggttg aaggcataat taatccggct
    10381 gtcgtcggta tcattcaata ccacgttgcg gagaaaaaca tccttagtat cgccgtcata
    10441 tgccttattg ccgtttttgt cgccgaaagc caacattccc cgccccttct tgctgaaatc
    10501 acatccattg ttgggcgcac cgtctttttt aacttgaaca ggacagatat agacagggag
    10561 attgagccga acggcttcgc ccctggaaaa acgcaaaagg ttggcaacct gctccgcgtg
    10621 actggcaatg cggcgtgatg caatccaccc gcccatattg gggagggcta tcgtcgccat
    10681 aatggctgca atggccatca cgatgagcag ctctattagc gtgaaacctt gttgttttcg
    10741 tgtacacata agcaatagga cgttaattgg taatgtatag tggattaaat tcaaaccggt
    10801 acggcattgc c
//
LOCUS       DAWTQS010000065        10272 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.065, whole genome shotgun sequence.
ACCESSION   DAWTQS010000065 DAWTQS010000000
VERSION     DAWTQS010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10272
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.065"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(205..681)
                     /gene="greA"
                     /locus_tag="WNA25_001739"
     CDS             complement(205..681)
                     /gene="greA"
                     /locus_tag="WNA25_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFA9824442.1"
                     /translation="MQKIPLTVRGAELLKQELQQLKSVARPEVIEAIAEARSHGDLSE
                     NAEYEAAKERQGFIEGRISELEHKLSVAHIINPAEIHAEGKIVFGTTVTLEDLETEEH
                     VTYQIVGEDEADIKQGKIYVGSPIARALIGKEEGDTAEVQAPGGVREYDIIEVRYI"
     gene            1072..2373
                     /gene="aroA"
                     /locus_tag="WNA25_001740"
     CDS             1072..2373
                     /gene="aroA"
                     /locus_tag="WNA25_001740"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFA9824443.1"
                     /translation="MTESVRLPAASLKPSTVALPGSKSISNRTLLLAALSDNVCEIHS
                     LLKSDDTDRMLEALDKLGVQIEHLAEGRLKVHGTGGRFPNRSADLFLGNAGTAFRPLT
                     AALAVLGGDYHLHGVPRMHERPIGDLVDALRIAGADVEYLGNEHYPPLHIGKRQDCGE
                     RVIPIKGNVSSQFLTALLMALPLTGQAFEIRMVGELISKPYIDITLKLMAQFGVQVAN
                     EGYRVFKIPADAHYHAPEHLHVEGDASGASYFLAAGLIAATPVRVTGIGANSIQGDVA
                     FARELEKIGADVVWGENFVEVSRPKGRAVQAFDLDANHIPDAAMTLAIVALATRQTCT
                     LRNIGSWRVKETDRIAAMANELRKLGAKVVEEAEAIHITPPETPTPDAVIDTYDDHRM
                     AMCFSLISLLGVPVVINDPKCTHKTFPTYFEVFSSLTETAE"
     gene            complement(2451..2984)
                     /locus_tag="WNA25_001741"
     CDS             complement(2451..2984)
                     /locus_tag="WNA25_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFA9824444.1"
                     /translation="MFSPDKTLFLCLGALLLASCGTTSGKHRQPKPKQTVRQIQAVRI
                     SHIGRTQGSQELMLHSLGLIGTPYKWGGSSTATGFDCSGMIQFVYKNALNVKLPRTAR
                     DMAAASRKIPDSRLKAGDLVFFNTGGAHRYSHVGLYIGNGEFIHAPGSGKTIKTEKLS
                     TPFYAKNYLGAHTFFTE"
     gene            complement(3041..4567)
                     /locus_tag="WNA25_001742"
     CDS             complement(3041..4567)
                     /locus_tag="WNA25_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003765285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HFA9824445.1"
                     /translation="MKTRSLISLLCLLLCSCSSWLPPLEERTESRHFNTSKPVLLDNI
                     LQIRHTPHNNGLSDIYLLDDPHEAFAARAALIESAEHSLDLQYYIWRNDISGRLLFNL
                     MYLAAERGVRVRLLLDDNNTRGLDDLLLALDSHPNIEVRLFNPFVLRKWRALGYLTDF
                     PRLNRRMHNKSFTADNRATILGGRNIGDEYFKVGEDTVFADLDILATGSVVGEVSHDF
                     DRYWASHSAHNATRIIRSGNIGKGLQALGYNDETSRHALLRYRETVEQSPLYQKIQTG
                     RIDWQSVQTRLISDNPAKGLDRDRRKPPIAGRLQDALKQPEKSVYLVSPYFVPTKSGT
                     DALAKLVQDGIDVTVLTNSLQATDVAAVHSGYVKYRKPLLKAGIKLYELQPNHAVPAT
                     KDKGLTGSSVTSLHAKTFIVDGKRIFIGSFNLDPRSARLNTEMGVVIESPKIAEQMER
                     TLADTTPEYAYRVTLDKHNRLQWHDPATRKTYPNEPEAKLWKRIAAKILSLLPIEGLL
                     "
     gene            complement(4679..6220)
                     /locus_tag="WNA25_001743"
     CDS             complement(4679..6220)
                     /locus_tag="WNA25_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFA9824446.1"
                     /translation="MIFDNNYHYNAMPIRLLVNIQDADSAGIQTASENNNGTRMGYNH
                     PMEKNTLSARAPSPWLPLLLAIAIFMQMLDATILNTALPEIAADLNESPPDMQQAVVA
                     YTLTVALLIPLSSYLADKFGTKKVFFGSIAVFMLGSALCAASGSLFELALSRVVQGIG
                     GSMLVPIPRLTILRVYEKSKLLNAINYAVMPALIGPALGPLAGGYLVEYASWHWIFLL
                     NLPIGLLGFILGRNIMPDVKGNDTALDFKGYLTFSVAACLLLLAAESLSHALPPYFAL
                     LPLCGGLLFARRYFRHMKTASKPIYSADLFLIRTFRLGLAGNLFSRLGISSIPFLMPL
                     MFQVAFGFGASLSGWLVAPVALSSLLVKPLIAPLMKRFGYRTVLLWNTKLLAAFIMLL
                     ALPDGNSPLWIWIFLSLAIGACNSLQFSAMNTLTLADLRPQQTGSGNSLMAVNQQLAI
                     SVGIVAGALILKNWTFLIPASSGLHFAFRMTLLSIGGITLASSLVFKRLHVSDGANLT
                     RGTRP"
     gene            6368..7147
                     /locus_tag="WNA25_001744"
     CDS             6368..7147
                     /locus_tag="WNA25_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFA9824447.1"
                     /translation="MSATIPPKIIRYDSNPTDVYFFGTCVLDLFMPEAGMDAIALIEQ
                     QGIRVHFPMAQSCCGQPAYSSGHPTEAFDVAKAQLDLFPENWPIVVPSGSCGGMMKHH
                     WPTLFKNTEYESKAVDCANRIIEFTHFLLAIGYKPEDKGEPVKVAVHTSCAARREMNV
                     HLSGWQLIDGMENVERIVHDHESECCGFGGTFSVKQADISGAMVTDKVAALKETGATE
                     IISADCGCMMNIGGKIAKDEPDMPRPKHIASFLLERTGGKV"
     gene            7144..7845
                     /locus_tag="WNA25_001745"
     CDS             7144..7845
                     /locus_tag="WNA25_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFA9824448.1"
                     /translation="MSARENILAKLKKAGALPMEEPAVFDYYREKGVSWDSEAERLKH
                     WAAAMRAVKTEIYWVTKSNWMQVFREAAEGKGLKNILLPLATEHGQIARAALAGSNID
                     PIAFEREIDTWKTEFFTNIDAGFSGAQCGIARTGTLMLFSSPEEPRTLSLVPPVHFCL
                     FDTSKMYNEFHNAVEGEKLVENGMPTNVFLISGPSKTADIQLTLAYGAHGPRDLVILA
                     ILPDHISPADLEENA"
     gene            7842..9296
                     /locus_tag="WNA25_001746"
     CDS             7842..9296
                     /locus_tag="WNA25_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HFA9824449.1"
                     /translation="MTTQTIKFHMKPETFKQNAAISLQDKPLRKSLRTAMDMLMTKRK
                     AVLTDEEELQSLRDLCEHIRQRSLSKLPALLEQLEENLTKLGVKVHWAETPAEACQII
                     HDIITAKNGKLMVKGKSMVSEEIELNHYLEAKGIKAVESDLGEFIVQMAGEKPTHIVM
                     PAIHKTKEQVSELFHQNLGTPLTDDVDQLTGFARKALRDIYSTADVGLSGVNFAVAET
                     GTLCLVENEGNGRLSTTVPPVHIAITGIEKVVAKLSDVPPLYSLLPRSAIGQNITTYF
                     NMITGPRRSEELDGPQEMHLVLLDNGRSQAYAEDQMRRTLQCIRCGACMNHCPVYTRI
                     GGAAYGTTYPGPIGEIISPHLLGLDATRDLPTACTMCGACVEVCPVRIPITEQMQRLR
                     VEAQRSPTETVPHPIRGQGASHTFGEQMAWRTFNGIFSGSKTYRAFGWAATKFRNLTP
                     RKQLGWTQNRVPMKPAKKTLHELMAEKMRQKEQA"
     gene            complement(9445..9864)
                     /gene="fitB"
                     /locus_tag="WNA25_001747"
     CDS             complement(9445..9864)
                     /gene="fitB"
                     /locus_tag="WNA25_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin FitB"
                     /protein_id="HFA9824450.1"
                     /translation="MILLDTNVISEPLRPQPNERVVAWLDSLILEDVYLSAITVAELR
                     LGVALLLNGKKKNVLHERLEQSILPLFAGRILPFDEPVAAIYAQIRSYAKTHGKEIAA
                     ADGYIAATAKQHSLTVATRDTGSFFAADVAVFNPWHD"
     gene            complement(9864..10100)
                     /gene="fitA"
                     /locus_tag="WNA25_001748"
     CDS             complement(9864..10100)
                     /gene="fitA"
                     /locus_tag="WNA25_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin FitA"
                     /protein_id="HFA9824451.1"
                     /translation="MASVVIRNLSEATHNAIKFRARAAGRSTEAEIRLILDNIAKAQQ
                     TVRLGSMLASIGQEIGGVELEDVRGRNTDNEVSL"
BASE COUNT         2457 a         2667 c         2820 g         2328 t
ORIGIN      
        1 ttttgaaaaa taatatttca atataaaaca ataaattaac ttttttgaaa atgttgcttt
       61 cccgcaacat gatttaaata cagaccttgt caaagccgat gcggcgcaga cccggatgcc
      121 gtctcgtttt ttaaaacacc ataaaaaaac acacgcggta ctttaatttc ctgcgtgtgt
      181 cgagtgtatc gaaatcaagc cgaatcaaat ataccggact tcgataatat cgtattcgcg
      241 tacgccgccc ggtgcctgaa cttccgccgt atccccttct tccttgccga tcagggcgcg
      301 ggcaatcgga gagccgacat agattttgcc ctgtttgatg tcggcttcgt cttcgccgac
      361 aatttgatag gtaacgtgtt cttccgtttc caaatcttcc agcgtaaccg tcgtaccgaa
      421 cacgattttg ccttcggcgt ggatttcggc cggattgatg atgtgggcaa cggaaagttt
      481 gtgttccaac tcggaaatgc ggccctcgat aaaaccttgg cgttctttgg cggcttcgta
      541 ttcggcgttt tcggacaaat cgccgtgcga tctggcttcg gcaatcgctt cgatcacttc
      601 gggacgcgcc acgcttttga gctgctgcaa ttcctgtttc agcaattccg caccgcgtac
      661 ggtcaggggg attttttgca tcggtctttt tctccatatt ccggcacacc ggtttgcggc
      721 ggcaagcata ccgcgtaccg tcttgttttg tgcgtccgga tattaaaata aaaatacaag
      781 ccgcccggaa aatcggcggc ttgtctgtcg ttgaacagcg gctattctac caaattctat
      841 gaaattggca atcgtgccgt gccgccggca gacgcgctat gtccgcaaca aaagccgaaa
      901 atatgccggc aaaaaaattt tagaaacaaa aaatttaaaa aaaatcaatt ttcggcataa
      961 aaaaccaaat tcacgtactt taaaaccgaa aatgccaaac ctgagatttt ccagacggca
     1021 tttgcaccag tataatgcag gctgttttta tctttatatt tacgttttgc catgaccgaa
     1081 tccgtccgcc tccccgccgc ctcgctcaaa ccttccaccg tcgccctgcc cggctccaaa
     1141 agcatcagca accgcaccct gctgctcgcc gccctgtccg acaatgtttg cgaaatccat
     1201 tccctgctca aatccgacga taccgaccgt atgctcgaag cactcgataa actcggcgtt
     1261 caaatcgaac atcttgccga aggccgtctg aaagtacacg gcacgggcgg acgcttcccc
     1321 aaccgctctg ccgatttgtt tttgggcaac gcgggcacgg cgttccgtcc gctgaccgcc
     1381 gcgctggccg ttttgggcgg cgattatcat ctgcacggcg tgcctcgtat gcacgaacgc
     1441 cccatcggcg atttggtcga tgcgttgcgg attgccggcg ccgatgtcga atatctcggc
     1501 aacgaacact atccgccgct tcatatcggc aaacgtcaag actgcggcga gcgcgtgatt
     1561 cctattaaag gcaatgtgtc cagccagttt ctgaccgccc ttttgatggc gttgccgctg
     1621 accgggcagg cgtttgaaat ccgtatggtc ggcgagttga tttccaagcc ctatatcgac
     1681 attactttga aactgatggc gcaattcggc gtacaggttg ccaatgaagg ctaccgcgtt
     1741 ttcaaaatcc ctgccgatgc gcactatcac gcacccgaac acttgcacgt cgaaggcgat
     1801 gcctccggcg cgtcctactt cctcgcagcc ggtttgattg ccgccacgcc cgtccgcgtt
     1861 accggcatcg gcgcaaacag catacagggc gatgtcgcct ttgcccgcga actggaaaaa
     1921 atcggtgcgg acgtggtttg gggcgaaaac ttcgtcgaag tctcgcgccc gaaaggacgc
     1981 gccgtccaag cctttgattt ggatgcgaac catatccccg atgccgcgat gaccctcgcc
     2041 atcgtcgcgc ttgccacaag gcaaacctgc acgcttcgca acatcggttc gtggcgcgtt
     2101 aaggaaaccg accgcatcgc cgcaatggcg aacgagttgc gcaaactcgg tgcaaaagtc
     2161 gtcgaagaag ccgaagcgat tcacattacc ccgcccgaaa cgccgacacc cgatgccgtc
     2221 atcgacactt acgacgacca ccgcatggcg atgtgtttct cgctgatttc gctgttggga
     2281 gtacccgtcg tcatcaacga cccgaaatgc acccataaaa ccttcccgac ttattttgaa
     2341 gtgttctcat cactgaccga aacagcggaa taaggcggca aaatgccgtc tgaaacccga
     2401 aaacagggct tcagacggca ttttgccgcg attccggcgc ggcgggcggc tcattctgta
     2461 aaaaacgtat gcgctccaag gtagtttttg gcgtaaaacg gtgtggagag tttttcggtt
     2521 ttgatggttt tgccgctgcc gggggcatgg atgaattcgc cgttgccgat gtagagtccg
     2581 acgtgtgagt agcggtgtgc gccgccggtg ttgaagaata cgaggtcgcc ggccttgagg
     2641 cggctgtcgg ggattttgcg gcttgccgcc gccatgtcgc gggcggtgcg cggcagcttg
     2701 acgttgaggg cgtttttgta aacgaattga atcatgccgc tgcagtcgaa gccggttgcg
     2761 gtgctgctgc cgccccattt gtagggcgtg ccgatgagtc cgaggctgtg gagcatgagt
     2821 tcctgcgagc cttgtgtgcg gccgatgtgg ctgatgcgga cggcttggat ttgccggact
     2881 gtctgtttgg gtttcggttg gcggtgtttg ccggaggtcg tgccgcatga ggcgaggagc
     2941 agtgcgccga gacagaggaa aagggttttg tcgggggaaa acatggtttt ttcctttgcg
     3001 ggttcggata tgccgtctga aggtgtttca gacggcattt ctataataaa ccttcgatgg
     3061 gcagcaggga taggattttt gcggcgatgc gtttccaaag tttggcttcg ggttcgttcg
     3121 ggtaggtttt tcgggtggcg ggatcgtgcc attgcaggcg gttgtgtttg tcgagggtaa
     3181 cgcggtaggc gtattcgggt gtggtatcgg cgagggtgcg ctccatctgt tctgcgattt
     3241 tggggctttc gatgacgacg cccatttcgg tattgagccg tgcggaacgg gggtcgaggt
     3301 tgaatgagcc gatgaagatg cgtttgccgt ccacaatgaa ggttttggca tgcaggctgg
     3361 ttacggagct gccggtcagg cctttgtctt ttgtggcggg gacggcatgg ttgggttgca
     3421 gctcgtagag tttgatgccg gctttgagca gcggttttcg gtatttgacg tagccggaat
     3481 ggacggcggc aacgtcggtc gcctgtagcg agttggtcag gacggtaacg tctatgccgt
     3541 cctgcaccag ttttgccagt gcgtctgtgc cggattttgt agggacgaaa tagggtgaaa
     3601 ccagatagac gcttttttcg ggctgtttga gcgcgtcttg cagcctcccg gcaatcggcg
     3661 gtttgcggcg gtcgcggtcg agtccttttg cagggttgtc gctgatcagg cgggtttgga
     3721 cgctctgcca gtcgatgcgt cccgtctgta ttttttggta gaggggcgac tgttcgacgg
     3781 tttcgcggta gcgcaggagc gcgtgtctgg atgtttcgtc gttgtatccg agtgcttgaa
     3841 gacccttgcc gatgttgccg ctgcggatga tgcgcgtggc gttgtgggcg gaatggcttg
     3901 cccagtagcg gtcgaagtcg tgcgatactt cgccgacgac gctgccggtg gcgaggatgt
     3961 ccaggtcggc gaaaacggtg tcctcaccga ctttgaagta ttcgtcgccg atattgcgtc
     4021 cgccgagtat ggtggcgcgg ttgtcggcgg taaaggattt gttgtgcatg cggcggttga
     4081 ggcgggggaa gtcggtcagg tagccgagtg cgcgccattt gcgtaggacg aaggggttga
     4141 acaggcgcac ttcgatattg ggatggctgt cgagggcgag caggagatcg tccaacccgc
     4201 gcgtgttgtt gtcgtccaac agcaggcgta cgcgcacgcc gcgttctgcg gcaaggtaca
     4261 tgaggttgaa cagcagcctg ccggaaatgt cgttgcgcca aatgtagtat tgcaaatcga
     4321 ggctgtgttc ggcagattcg ataagggcgg cgcgggcggc aaaggcttcg tgggggtcgt
     4381 cgagcaggta gatgtcggat agcccgttgt tatgaggggt gtgccggatt tgcaggatgt
     4441 tgtccaggag gacaggtttg gaagtattga aatgacggct ttccgtccgt tcttccagtg
     4501 ggggcaacca tgaagaacat gaacagagaa ggaggcataa aagggaaatg aggctgcgtg
     4561 ttttcatcgg ggatatagtt tcagacggca ttgcctgtat tttggggtct gtatgcattg
     4621 aaatgaagca tcataatcta aaggttggaa tgggtaaaag ttttgtcggg atgcggggtt
     4681 aaggccgcgt gcctcttgtc aggtttgcac cgtctgaaac gtgcagacgc ttaaaaacca
     4741 acgacgaggc aagggtgatg ccgccgatac tgagcagggt catacggaag gcgaaatgca
     4801 ggcctgaaga agccggtatc agaaatgtcc agtttttaag gattaatgcg ccggcaacaa
     4861 tgcccacgct gatggcaagc tgttggttga ccgccatcag gctgttgccg ctgccggtct
     4921 gttgcgggcg caaatcggcg agggtcagtg tgttcatggc agaaaactgt agggagttgc
     4981 atgcgccgat cgccagcgag aggaaaatcc aaatccacag cggcgagttt ccgtcaggca
     5041 gggcgagcag catgatgaag gcggcaagca gcttggtgtt ccaaagcagt accgtgcggt
     5101 agccgaaacg tttcatgagc ggtgcaatca gcggtttgac cagcagcgaa gacagggcga
     5161 cgggtgcgac cagccagccc gacaggcttg cgccgaagcc gaaagcgact tgaaacatca
     5221 ggggcatcag aaaaggaatc gagctgatgc cgagacggct gaacagattg cccgccagtc
     5281 ccaaacggaa ggtgcgtatc agaaacaggt cggcggaata aatcggtttg gacgcggttt
     5341 tcatatgtcg gaaataacgg cgtgcaaaca gcagtccgcc gcacagcggt aacagtgcaa
     5401 aatacggagg cagcgcgtgc gacaggcttt ctgccgcaag cagcagcagg caggcggcaa
     5461 cggaaaaggt cagatagcct ttgaaatcca gagcggtatc gttgcctttg acatcgggca
     5521 tgatgttgcg tcccaatatg aaacccagca gaccgatggg caggttgagc aggaaaatcc
     5581 agtgccacga agcgtattcg accagataac cgcccgccag aggtcccaac gcagggccga
     5641 tcagtgcagg catgaccgca taattgatgg cgttgagcag cttcgatttt tcatatacac
     5701 gcaagatggt cagacgcggt atcggaacca gcatcgaacc gccgatcccc tgaacgacac
     5761 gggaaagcgc caattcaaac agcgaacccg atgcggcgca caatgccgat ccgagcataa
     5821 aaacggcaat cgaaccgaaa aagacttttt tcgttccgaa cttgtccgcc aaataactgc
     5881 tcaaaggaat cagcagggca accgtcagcg tataggcaac gactgcctgt tgcatatccg
     5941 gaggcgattc gttcaggtcg gcggcaattt cgggcagtgc ggtatttaaa atggtcgcat
     6001 ccaacatctg cataaaaatg gcaattgcca gcagaagcgg cagccaaggg gatggtgcgc
     6061 gggcggacag ggtgtttttt tccatagggt gattgtaccc catccttgtg ccgttattgt
     6121 tttcagatgc tgtctgaatg ccggcagagt cggcatcctg aatgttcaca agcaaacgaa
     6181 tcggcattgc attgtaatga taattattat cgaaaatcat cagagtaagg tacagtaagc
     6241 gttatggggc agtttgtaag aaaaaccgga ttatttttta aaattagact tgacccgcaa
     6301 cggtcaatta cttaaagtaa acgcttacct ttctacagaa aaaacgggtt tcccgttatc
     6361 aaaaaatatg agcgcaacca ttcccccaaa aatcatccga tacgacagca atccgacaga
     6421 tgtctatttt ttcggcactt gcgtccttga tctttttatg cccgaagcag gcatggatgc
     6481 cattgcccta atcgagcagc agggcatacg cgtccatttc ccgatggcgc aaagctgctg
     6541 cggtcagcct gcctattcat ccggccatcc gaccgaagcc ttcgatgtcg ccaaagcgca
     6601 actcgacctt ttccccgaaa actggccgat tgtcgtaccg tccggctcat gcggcggcat
     6661 gatgaaacat cactggccta ccctgtttaa aaatacagaa tacgaatcca aagcggttga
     6721 ttgtgccaac cgtatcattg agtttactca tttcttgctt gccatcggct acaagcctga
     6781 agacaaaggc gagccagtca aagtcgccgt tcacacttcc tgtgccgccc gccgagaaat
     6841 gaatgtccat ctttcaggct ggcagttgat tgacggtatg gaaaacgtcg aacgcatcgt
     6901 ccacgaccac gaaagcgagt gttgcggctt cggcggcaca ttctccgtca aacaagccga
     6961 tatttccggc gcgatggtaa ccgacaaagt tgccgcgctg aaagaaaccg gcgcaaccga
     7021 aatcatcagc gcggactgcg gctgtatgat gaacatcgga ggcaaaatcg ccaaggacga
     7081 gccggatatg ccgcgtccga agcatatcgc atccttcttg ttggaacgca ccggaggcaa
     7141 agtatgagcg cgcgcgaaaa tattttggca aaactgaaaa aagccggcgc attgccgatg
     7201 gaagagcctg cggtttttga ttattaccgc gaaaagggcg tttcttggga cagcgaagcc
     7261 gagcgtctga aacattgggc tgccgccatg cgcgcggtca aaaccgaaat ctattgggtg
     7321 acgaaaagca attggatgca ggttttccgc gaagcggcag aaggcaaggg tttaaaaaac
     7381 atcctgctgc ccttggcgac cgaacacgga caaattgccc gtgccgcatt ggcgggcagc
     7441 aatatcgacc cgattgcctt cgagcgcgaa atcgatactt ggaaaaccga gtttttcacg
     7501 aacatcgatg caggcttcag cggcgcgcaa tgcggcatcg cccgcaccgg cacgctgatg
     7561 ctgttttcca gccctgaaga accgcgtact ttgagcctcg ttccgcccgt gcatttctgc
     7621 ctgttcgata cgtccaagat gtacaacgag tttcacaacg ccgtcgaagg cgaaaaactg
     7681 gtggaaaacg gtatgccgac caatgtattc ctgatttccg gcccgtccaa aaccgcagac
     7741 atccagttga cgctcgctta cggtgcgcac ggtccgcgtg atttggtcat cctcgccatt
     7801 ttgcccgacc acatttcccc tgccgatttg gaggaaaacg catgactacg caaaccatca
     7861 agttccacat gaagccggaa actttcaaac aaaacgccgc aatttccctt caagacaagc
     7921 ccttgcgtaa aagcctgcgt accgcgatgg atatgctgat gaccaagcgc aaagccgttt
     7981 tgaccgacga agaagagttg caaagcctgc gcgatttgtg cgaacacatc cgtcagcgtt
     8041 ccctctccaa attacccgcg ctgctggagc agttggaaga aaacctgact aagttgggcg
     8101 tgaaagtaca ctgggcggaa acccctgccg aagcctgcca aattatccac gacatcatca
     8161 cagccaaaaa cggcaagctg atggtcaaag gcaaatccat ggtcagcgaa gaaatcgagc
     8221 tgaaccatta tcttgaagca aaaggcatta aggcggtgga aagcgacttg ggcgagttta
     8281 tcgtccaaat ggcaggcgaa aaaccgaccc atatcgtgat gcccgcaatc cataaaacca
     8341 aagaacaggt cagcgaactg ttccaccaaa acctcggcac accgcttaca gacgacgtgg
     8401 accaactgac cggattcgcc cgcaaagccc tgcgcgatat ttacagcact gccgatgtcg
     8461 gcttgagcgg cgtaaacttc gccgttgccg aaacgggcac gctgtgtctg gtcgaaaacg
     8521 aaggcaacgg ccgcttgagt actaccgttc cgcccgtaca tatcgccatt acgggtattg
     8581 aaaaagttgt cgccaagctg tctgatgttc cgcctttgta cagtctgctg ccgcgttccg
     8641 ccatcggtca gaacattacc acttatttca atatgattac cggcccgcgc cgcagtgaag
     8701 aattagacgg tccgcaggaa atgcacttgg ttttgctcga caacggccgc agccaagctt
     8761 atgccgaaga ccaaatgcgc cgcaccctgc aatgtatccg ttgcggcgcg tgtatgaacc
     8821 attgcccggt ttatacacgc atcggcggcg cggcatacgg cacaacctat cccggtccga
     8881 ttggcgagat tatttccccg cacctgttgg gtcttgatgc cacccgcgac ctgccgaccg
     8941 cctgcacgat gtgcggcgcg tgcgtggaag tttgtccggt acgcatcccg attaccgaac
     9001 aaatgcaacg tttgcgcgtt gaagcacaac gttcgccgac cgaaaccgtg ccgcacccca
     9061 tccgagggca aggcgcatcg cataccttcg gcgaacaaat ggcgtggcgc acattcaacg
     9121 gtattttcag cggtagcaaa acctaccgcg ccttcggttg ggcagccacc aagttccgca
     9181 atctgacccc gcgcaaacag ttgggttgga cgcaaaaccg cgtaccgatg aaaccggcga
     9241 agaaaaccct gcacgaactg atggcggaaa aaatgcgcca aaaagaacag gcataaaaag
     9301 ttgttcgcaa aaataccgtc tgaaacccga aaacagggct tcagacggcc tgaaaagcag
     9361 cctgaacgcg caaaaataaa taatgaaacg gaatgctaag acgctttcag gcagcctgac
     9421 attaaggctg cttaaaagcg tcttttaatc gtgccacgga ttgaacaccg cgacatcggc
     9481 cgcaaaaaat gagccggtat cacgcgtagc aactgtcaaa ctgtgctgtt ttgcagtggc
     9541 ggcaatatag ccgtctgcgg cagcaatctc tttgccatgt gttttggcat aggaacgaat
     9601 ttgcgcatag attgcggcaa ccggttcatc aaaaggcaga atccgccccg caaataaagg
     9661 caaaatggat tgttccaaac gttcgtgcag cacattcttt ttcttgccat tgagcaacaa
     9721 cgccacaccc aaacgcaatt ctgcaacagt aatggcagac aaatacacat cttccaatat
     9781 caaactatcc aaccatgcca ccacacgttc attgggttgt gggcgcaaag gttcggaaat
     9841 cacattcgtg tccagcaaaa tcatcacaaa gaaacctcgt tatcagtatt acgaccgcgt
     9901 acgtcttcca gctcaacacc tccgatttcc tgccctattg atgccaacat tgaccccaaa
     9961 cgtacagttt gttgtgcttt ggcgatgtta tccaaaatta agcggatttc tgcttcggta
    10021 ctgcgccctg cggctcgcgc acggaatttg attgcgttgt gcgtggcctc ggataaattt
    10081 ctaatcacaa cagaagccat tgcctttcct tcctgaaaaa agtttttcaa atgctatcaa
    10141 aataaaaaaa atgatagcaa tctatttttt aacgcctgcg gatgcctgaa aattcaggtg
    10201 gccgcaggcg tttttttaca tctaaaaaaa cgtctcccct ccctctttct gataaataaa
    10261 aaacagtcgc tc
//
LOCUS       DAWTQS010000066        10246 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.066, whole genome shotgun sequence.
ACCESSION   DAWTQS010000066 DAWTQS010000000
VERSION     DAWTQS010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10246
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.066"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(<1..153)
                     /locus_tag="WNA25_001749"
     CDS             complement(<1..153)
                     /locus_tag="WNA25_001749"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HFA9824452.1"
                     /translation="MLSSGVNNEIKNKKLSLWAKREAGSVKWFCGQPVKRNDAANDDV
                     TDDAGTD"
     gene            complement(300..680)
                     /locus_tag="WNA25_001750"
                     /pseudo
     CDS             complement(300..680)
                     /locus_tag="WNA25_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            complement(709..1296)
                     /locus_tag="WNA25_001751"
     CDS             complement(709..1296)
                     /locus_tag="WNA25_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HFA9824453.1"
                     /translation="MPVFECGRQRVFIRQPAAPAIDQNRTRLHLPQFVFAGHYPNHGK
                     WPENNGAAGVASPASDIKGKYVQSVTVANGVVTAQMKSDGVNKEIKNKKLSLWARREA
                     GSVKWFCGQPVTRDKAVTDDAVKDVTGNDKIETKHLPSTCRCFQIGSVKWFCGQPVTR
                     DNAGTDAVTADTTGKDKEIDTKHLPSTCRDKSSAE"
     gene            1189..1497
                     /locus_tag="WNA25_001752"
                     /pseudo
     CDS             1189..1497
                     /locus_tag="WNA25_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228827.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
     gene            complement(1572..3626)
                     /gene="metG"
                     /locus_tag="WNA25_001753"
     CDS             complement(1572..3626)
                     /gene="metG"
                     /locus_tag="WNA25_001753"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFA9824454.1"
                     /translation="MTRKILVTSALPYANGSIHLGHMVEHIQTDVWVRFQKLRGHECY
                     YCCADDTHGTPVMLAAQKQGIAPEDMIAKVRKEHLADFTGFFIGYDNYYSTHSTENKQ
                     FSQDIYRALKANGKIESRVIEQLFDPEKQMFLPDRFVKGECPKCHAQDQYGDNCEVCG
                     TTYSPTELINPYSAVSGAKPELRESEHFFFKLGECADFLKAWTSGNNPHDGKPHLQPE
                     ALNKMKEWLGEGGETTLSDWDISRDAPYFGFEIPDAPGKYFYVWLDAPVGYMASFKNL
                     CDRIGIDFDEYFKADSQTEMYHFIGKDILYFHALFWPAMLHFSGHRAPTGVYAHGFLT
                     VDGQKMSKSRGTFITAKSYLEQGLNPEWMRYYIAAKLNSKIEDIDLNLQDFISRVNSD
                     LVGKYVNIAARASGFIAKRFEGRPKDVSGSALLAKLAAESDTIAEQYENREYARALRD
                     IMALADIVNEYVDANKPWELAKQEGQDERLHEVCSELINAFTMLTAYLAPVLPQTAAN
                     AARFLNLDAITWKNTRETLGEHAINKYEHLMQRVEQKQVDDLIEANKQSIQTASAPVE
                     EGKYEKVAEQAGFDDFMKIDMRVAKVLNCEAVEGSTKLLKFDLDFGFEKRIIFSGIAA
                     SYPNPAELNGRMVIAVANFAPRKMAKFGVSEGMILSAATADGKLKLLDVDAGAQPGDK
                     VG"
     gene            complement(3742..5580)
                     /gene="glmS"
                     /locus_tag="WNA25_001754"
     CDS             complement(3742..5580)
                     /gene="glmS"
                     /locus_tag="WNA25_001754"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFA9824455.1"
                     /translation="MCGIVGAIRAHHNVVDFLTDGLKRLEYRGYDSSGIAVYSDGKIK
                     RVRRVGRVQLMEDAAREKGISGGIGIGHTRWATHGGVTEPNAHPHISGGMIAVVHNGI
                     IENFESERKRLEGLGYRFESQTDTEVIAHSINHEYAQNGGKLFEAVQEAVKRFHGAYA
                     IAVIAQDKPDELVVARMGCPLLVALGDDETFIASDVSAVIAFTRRVAYLEDGDIALLA
                     SDGIKRLTDKNGLPAERKVKVSELSPASLEPGPHSHFMQKEIHEQPRAIADTAEVFLD
                     GGFIPENFGKNAKSVFESIRSVKILACGTSYYAALTAKYWLESIAKIPADVEIASEYR
                     YRSVIADPDQLVITISQSGETLDTMEALKYAKSLGHRHSLSVCNVMESALPRESSLVL
                     YTRAGAEIGVASTKAFTTQLVVLFGLAVTLAKVRGVVSGEDEARYTEELRQLPGSVQH
                     ALNLEPQIAAWAQQFAKKTSALFLGRGIHYPIALEGALKLKEITYIHAEAYPAGELKH
                     GPLALVDENMPVVVIAPNDSLLDKVKANMQEVGARGGELFVFADLDSNFNAAEGVHVI
                     RAPRHVGELSPVVHTVPVQLLAYHTALARGTDVDKPRNLAKSVTVE"
     gene            5852..6358
                     /locus_tag="WNA25_001755"
                     /pseudo
     CDS             5852..6358
                     /locus_tag="WNA25_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224225.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6662..7987)
                     /locus_tag="WNA25_001756"
     CDS             complement(6662..7987)
                     /locus_tag="WNA25_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFA9824456.1"
                     /translation="MKKHLLRSALYGIAAAILAACQSRSIQTFPQPDTSVINGPDRPA
                     GIPDPAGTTVAGGGAVYTVVPHLSMPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCA
                     QAFQTPVHSFQAKRFFERYFTPWQVAGNGSLAGTVTGYYEPVLKGDGRRTERARFPIY
                     GIPDDFISVPLPAGLRGGKALVRIRQTGKNSGTIDNAGGTHTADLSRFPITARTTAIK
                     GRFEGSRFLPYHTRNQINGGALDGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIR
                     IGYADKNEHPYVSIGRYMADKGYLKLGQTSMQGIKAYMRQNPQRLAEVLGQNPSYIFF
                     RELAGSGNEGPVGALGTPLMGEYAGAIDRHYITLGAPLFVATAHPVTRKVLNRLIMAQ
                     DTGSAIKGAVRVDYFWGYGDEAGELAGKQKTTGYVWQLLPNGMKPEYRP"
     gene            8220..9039
                     /locus_tag="WNA25_001757"
                     /pseudo
     CDS             8220..9039
                     /locus_tag="WNA25_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285760.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family iron permease"
     gene            9036..10202
                     /locus_tag="WNA25_001758"
     CDS             9036..10202
                     /locus_tag="WNA25_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFA9824457.1"
                     /translation="MRKFNLTALSVMLALGLTACQPPEAEKAAPAASGETQSANEGGS
                     VGIAVNDNACEPMNLTVPSGQVVFNIKNNSGRKLEWEILKGVMVVDERENIAPGLSDK
                     MTVTLLPGEYEMTCGLLTNPRGKLVVADSGFKDTANEADLEKLPQPLADYKAYVQGEV
                     KELAAKTKTFTEAVKAGDIEKAKSLFADTRVHYERIEPIAELFSELDPVIDACEDDFK
                     DGAKDAGFTGFHRIEHALWVEKDVSGVKETAAKLMTDVEALQKEIDALAFPPGKVVGG
                     ASELIEEAAGSKISGEEDRYSHTDLSDFQANADGSKKIVDLFRPLIEAKNKALLEKTD
                     TNFKQVNEILAKYRTKDGFETYDKLSEADRKALQAPINALAEDLAQLRGILGLK"
BASE COUNT         2056 a         2545 c         3174 g         2471 t
ORIGIN      
        1 gtcggtgccg gcgtcgtcgg taacgtcgtc gttggcggcg tcgttgcgct taaccggctg
       61 tccgcagaac cattttaccg aaccggcttc acgcttggcc cacagggaga gttttttgtt
      121 tttgatttca ttgtttacgc cgcttgaaag cattttggcg gtaacgacgc ctttttcgac
      181 cgtaacgctt ttaacatatt tgcctttgat gtcggagggg gggatgccac gccggcagaa
      241 gcgttgtttt ccggccattc gccgtgattc aggtaatatt cgggtgtttt tgtgcaaccg
      301 gcagttgatt tatcgcggca ggttgacggc aggtgcttgg tttcgatgtt ggtgccgttg
      361 ccggcgtcgt tggcggcggt gacggtgtcg tctttggcgt tggcttcggt gcgcgtaacc
      421 ggctgtccgc agaaccattt taccgaaccg tcttgacgcc tggcccacag ggagagtttt
      481 ttgcctttga tttctttgtt tacgccggtt gaagccattt gggcggtgac gacgcctttt
      541 gcgacttcaa ctttctgaac atatttgcct ttgattgttg aagcggatgc cacgccggca
      601 gcgccgttgt ttgcgggcca tttgccgtta ttcgggtaat attcggtgac ggctgagggt
      661 aatacccggt gatggctgat ttatttgaaa tttaaggcct aatttgcctt attcggcaga
      721 tgatttatcg cggcaggttg acggcaggtg cttggtgtcg atttctttgt ctttgccggt
      781 ggtgtcggcg gtgacggcgt cggtgccggc gttgtcgcgc gtaaccggct gtccgcagaa
      841 ccattttacc gaaccgattt gaaagcaacg gcaggttgac ggcaggtgct tggtttcgat
      901 tttgtcgttg ccggtgacgt ctttgacggc gtcgtcggtg acggctttgt cgcgcgtaac
      961 cggctgtccg cagaaccatt ttaccgaacc ggcttcacgc ctggcccaca gggagagttt
     1021 tttgtttttg atttctttgt ttacgccgtc tgatttcatt tgggcggtaa cgacgccgtt
     1081 tgcgaccgta acgctttgaa catatttgcc tttgatgtcg gaggcggggg atgccacgcc
     1141 ggcagcgccg ttgttttccg gccatttgcc gtgattcggg taatgtccgg cgaaaacgaa
     1201 ttgcggcaga tgaagccggg tgcggttttg atcaattgcg ggcgcggcgg gctggcggat
     1261 gaaaacgcgc tgcctgccgc actcaaatac gggcagatcg gcggggcggg tgtcgatgtt
     1321 ttgacggagg aaccgccgag gggcggcaat cccttgctga atgcacgatt acccaatctg
     1381 attgttacgc cgcataccgc gtgggcgagc cgcgaggctt tggatcggtt gtttgaaata
     1441 ttgttggcga acattcacgc ctttgtgaaa ggcgaggcgc aaaaccgcgt ggtttgaacc
     1501 tgtcgggatt gcggaaaaaa acgccgtctg aacgcccaag gggttcagac ggcgtttttt
     1561 gagatttctg cctaaccgac tttgtcgccc ggctgagcgc ccgcatccac gtcaagcagc
     1621 ttcagtttgc cgtctgccgt ggcggcggag aggatcatac cttcggatac gccgaatttt
     1681 gccattttgc gtggggcgaa gttggcgact gcgatgacca tgcgaccgtt caattcggca
     1741 ggatttgggt aagacgcggc gatgccggag aagatgatgc gtttttcaaa accgaaatcg
     1801 aggtcgaatt tcaaaagttt ggtgctgcct tcgacggctt cgcagttcaa tactttggcg
     1861 acgcgcatat cgattttcat aaagtcgtcg aagcccgcct gttcggcgac tttttcgtat
     1921 ttaccctctt caacgggcgc ggatgccgtc tgaatgcttt gtttgttggc ttcgatcaaa
     1981 tcgtccactt gtttttgctc cactcgttgc attaaatgtt cgtatttgtt gatggcgtgt
     2041 tcgcctaggg tttcgcgcgt gttcttccag gtaatcgcgt ccagattgag gaaacgcgcg
     2101 gcgttggcgg cggtttgcgg caacacgggg gcgaggtagg cggtcagcat ggtgaaggcg
     2161 ttgatgagtt cgctgcatac ttcgtgcagg cgttcgtctt gaccttcttg tttggcgagc
     2221 tcccacggtt tgttggcatc gacgtattcg ttgacaatgt ctgccaatgc catgatgtcg
     2281 cgcagggcgc gggcgtattc gcggttttcg tattgctcgg cgatggtgtc gctttcggcg
     2341 gcgagttttg ccagcaatgc gctgcctgaa acgtctttcg gacgaccttc aaagcgtttg
     2401 gcgatgaaac ccgaagcgcg ggcggcgatg ttgacgtatt tgccgacaag gtcgctgttg
     2461 acgcgcgaaa taaagtcttg caggttcaaa tcgatgtctt cgattttgct gttgagcttg
     2521 gcggcgatgt agtagcgcat ccactcgggg ttcaggcctt gttccaaata ggatttggcg
     2581 gtgatgaagg tgccgcgcga tttggacatt ttttgtccgt ccacggtcaa aaagccgtgt
     2641 gcgtacacgc cggtcggggc gcggtggccg gagaaatgca gcatggcggg ccagaacagg
     2701 gcgtggaaat agaggatgtc tttgccgatg aagtggtaca tctcggtttg gctgtcggct
     2761 ttgaagtatt cgtcgaaatc gatgccgatg cggtcgcaca ggtttttaaa cgacgccata
     2821 tagccgacgg gcgcgtccag ccaaacgtag aagtatttgc ccggcgcgtc ggggatttca
     2881 aaaccgaaat acggcgcgtc gcgggaaatg tcccagtcgg acagggtggt ttctccgcct
     2941 tcgcccagcc attccttcat tttgttgagg gcttcgggtt gcagatgggg cttgccgtcg
     3001 tgcgggttgt tgccggaagt ccatgctttg aggaagtcgg cgcattcgcc cagtttgaag
     3061 aagaagtgtt cggattcgcg caattcgggt ttcgcgccgg aaacggcgga atacgggttg
     3121 atcagttcgg tcggggaata ggtcgtgccg cagacttcgc agttgtcgcc gtattggtct
     3181 tgggcgtggc atttcgggca ttcgcctttg acgaagcggt cgggcaggaa catttgtttt
     3241 tcggggtcga aaagctgctc gatgacgcgg ctttcgattt tgccgttggc tttcagcgcg
     3301 cggtaaatgt cttgggaaaa ctgtttgttt tcagtggaat gggtgctgta ataattgtcg
     3361 tagccgatga aaaagccggt aaagtcggca aggtgctctt tgcgcacttt ggcaatcatg
     3421 tcttcgggcg cgatgccttg tttttgcgcg gcaagcataa cgggcgtgcc gtgggtgtcg
     3481 tcggcgcagc agtagtagca ttcgtggccg cgcagttttt gaaagcgcac ccaaacgtcg
     3541 gtttggatgt gttcgaccat gtggccgagg tggatgctgc cgttggcata gggcagggcg
     3601 gaggtaacta agattttgcg tgtcatattg tgctttgcaa acaatgggta aaggcggatt
     3661 ataccgcaaa tcaaacgggg aaatgccgtc tgaagcctga aaaatcgggc ttcagacggc
     3721 atttttgcca accggcggga tttattcgac ggttacggat ttcgccaggt tgcgcggctt
     3781 gtccacatcc gtgccgcgcg cgagggcggt gtggtaggcg aggagctgca cggggacggt
     3841 atgcacgacg ggggacagtt cgccgacgtg gcgaggtgcg cggataacgt gcacgccttc
     3901 ggcggcatta aaattgctgt cgaggtcggc aaagacgaaa agttcgccgc cgcgcgcgcc
     3961 gacttcctgc atattggctt tgactttgtc caacaggctg tcgttgggcg cgatgacgac
     4021 gacgggcatg ttttcgtcca ccaaggcaag cggcccgtgt ttcagttcgc cggcaggata
     4081 ggcttcggcg tggatgtagg tgatttcctt tagcttcaac gcaccttcaa gggcaatcgg
     4141 gtaatggatg ccgcgcccca aaaacagcgc gctggttttc ttggcaaact gttgcgccca
     4201 agcggcaatt tgcggttcga ggttcaaagc gtgctgcacg ctgccgggaa gctggcggag
     4261 ttcttcggtg taacgtgctt catcttcgcc ggataccacg ccgcgcactt tcgccagcgt
     4321 taccgccaaa ccgaacagca cgaccagttg cgtggtaaat gctttagtcg aggcgacgcc
     4381 gatttccgca ccggcacggg tataaagcac aaggctgctt tcgcgcggca gggcggattc
     4441 catcacgttg cagacggaga ggctgtggcg gtgtcccaaa gatttggcgt atttcaaagc
     4501 ctccatcgtg tccagcgttt cgccggattg ggaaatggta atgaccagtt ggtcggggtc
     4561 ggcaatcacg ctgcggtacc ggtattcgct tgcgatttcg acgtcggccg ggattttggc
     4621 gatggattcc aaccaatatt tggcggtcag cgcggcgtaa taggacgtgc cgcaggcaag
     4681 gattttgacg ctgcggatgc tttcaaacac gcttttggcg tttttgccga agttttcggg
     4741 gatgaatccg ccgtcgagga aaacttccgc cgtgtccgca atcgcgcgcg gctgctcgtg
     4801 gatttctttt tgcataaagt ggctgtgcgg acccggttcc aaagaggcgg gcgagagttc
     4861 ggataccttg actttgcgtt cggcaggcag gccgttttta tcggtcagcc gtttgatgcc
     4921 gtctgaagcc agcagcgcga tgtcgccgtc ttcgaggtac gccacgcggc gcgtaaaggc
     4981 gatgacggcg gatacgtccg aagcgataaa ggtttcatcg tcgcccaaag cgaccaaaag
     5041 cgggcagccc atacgcgcca caactaattc atcaggcttg tcttgggcaa taaccgcgat
     5101 ggcgtatgcg ccgtggaaac gtttgaccgc ttcctgcacg gcttcaaaca atttgccgcc
     5161 gttttgcgcg tattcgtgat tgatgctgtg tgcgatgact tcggtatccg tttgcgattc
     5221 aaaccggtat cccaaacctt ccaagcgttt gcgttcgctt tcaaagtttt cgatgatgcc
     5281 gttgtgtacg accgcaatca taccgccgct gatgtgcggg tgggcgttcg gctcggtaac
     5341 gccgccgtgt gtcgcccagc gcgtatgccc gatgccgatg ccgccgctga tgcctttttc
     5401 gcgtgccgcg tcctccataa gctgcacgcg tccgacgcgg cgcacgcgct tgattttgcc
     5461 gtctgaatac acagcaatgc ctgatgagtc ataaccccgg tattcgaggc gtttgaggcc
     5521 gtcggtcaga aaatcgacga cgttgtgatg ggcgcggatg gcgccgacga taccgcacat
     5581 aaactgttcc ttagtatccg gttgaaaaaa aagatgcgga cggcttccgt accgcacctt
     5641 ccgtttcgga ttataaaccg cctcccgcgc cggaaaacag caaaatgccg tctgaaggct
     5701 tgggcttgct caaaaaaggg agggatttcc ccgcctgacc gggatgggcg ttcagacggc
     5761 attgcctgcc gccggtttat agtttttgca aatcaatatt aacaagctga aaaaaaacaa
     5821 tatacccgcc cggtcttaat gttaacggag tatggaaatg aaacaaatgc ttttggccgt
     5881 cggcgtggcg gcggtgttgg cgggctgcgg caaggatgcc ggcggttacg agggttattg
     5941 gcgcgaaaag tcggacaaaa aagagggcgt gattgccgtc aaaaaagaaa aaggcaatta
     6001 cttccttaat aaaatcaacg tgtttacagg caaggaggag tctttgcttt tgtctgaaaa
     6061 agacggcgcg ctttcgataa acacggggat aggggaaatc ccgatcaaac tttccgacga
     6121 cgggaaagag ctgtatgtcg aacgcaggcg gtatgtgaaa accgatgcgg cgatgaagga
     6181 caaaatcatc gcccaccaga aaaagtgcgg acaaacggca caggcatacc tcgacgcgcg
     6241 aaatgcgttg ccgtcaaacc aaacgtatca gcagcgtcag gcggcgatcg agcaattgaa
     6301 acggcggttt gaagccgagt ttgacgaatt ggaaaaagaa atcaaatgca acggcagaac
     6361 cccggcattg ttgctttggc aggggacaac cgggaggatg ccgccgtccg aatcggacgc
     6421 gtggtttcca taccggtacg ggcgggcggg aatgtccgcc tttttttgtt cggatgcgtt
     6481 tgaatacccg tccgattctg tccgtttgca agggggattt ccgttcgggc ggaaatccat
     6541 tccgtcaaat aagaaaggcg ttttgtccgg ccggccggct tgtgcctgcg gtatcgcaaa
     6601 acttcgcctt aatgcgcccg attggctagg gacgggcttc ggacggcatt gttttgccgg
     6661 ttcacgggcg gtattcgggc ttcatgccgt tgggcaggag ctgccagacg tatcccgtgg
     6721 ttttctgttt gccggcaagt tcgccggctt cgtcgccgta accccaaaaa taatccacgc
     6781 gcaccgcgcc tttgatcgcg ctgcctgtat cctgcgccat aatcaggcgg ttgaggactt
     6841 tgcgggtaac cggatgggcg gtggcgacaa ataagggcgc gcccaaggta atgtagtgcc
     6901 ggtcgattgc gccggcgtat tcccccatca gcggcgtgcc cagtgcgccg acggggccct
     6961 cattgccgct tccggcaagc tcgcggaaaa agatatagct ggggttttgc cccaaaactt
     7021 cggcgaggcg ttgcggattt tgccgcatat aggctttgat gccctgcatg gaggtttgcc
     7081 cgagcttgag gtagcctttg tccgccatat agcgtccgat ggaaacatac ggatgctcgt
     7141 ttttgtcggc atagccgatg cggatgtatt tgccggacgg ggttttcaga cggcccgagc
     7201 cttgtatgtg catgaaaaaa agttcgacgg ggtcttcggc gtaaccgagt atcggggctt
     7261 tgccgtcaag cgcgccgccg ttgatttggt ttcgcgtgtg gtaggggagg aagcggcttc
     7321 cttcaaacct gcccttgatt gccgttgtgc gcgcggtaat ggggaatcgg gaaaggtcgg
     7381 cggtatgcgt gccgccggca ttgtcgattg tgccgctgtt ttttcccgtc tgcctgatgc
     7441 ggacaagggc tttcccgccc cgcaaaccgg caggcagcgg gacggagata aaatcgtcgg
     7501 gaataccgta aatcgggaag cgggcccgtt ccgtccgcct gccgtcgccc ttcagcaccg
     7561 gctcgtaata gccggtaacc gtacctgcaa ggcttccgtt gcctgcaacc tgccacggcg
     7621 tgaaatagcg ttcaaaaaac cgctttgcct gaaaggaatg cacgggggtt tgaaaggctt
     7681 gggcgcacac atcctgccag ccttggcggt ttttcaaatt ggcgcagccg aggcggaagg
     7741 attgcaggct tttggcaaaa tcctgcgccg cccagtgggg catggacagg tgcggcacaa
     7801 cggtatagac ggccccgccg ccggcaaccg tcgttccggc ggggtcgggg atgccggccg
     7861 gccggtccgg gccgttgatg acggatgtgt cgggttgcgg aaaggtttgg atgctcctgc
     7921 tttggcaggc ggcgaggatg gcggcggcga tgccgtacag ggcggagcgg agcaggtgtt
     7981 ttttcataat ggacaatgtt gtcgtcagta ataaaaaaag atggtttcgg gcggcgttgc
     8041 ggtagccggg gagaggggat tttaacacag ggcgcagccg cagcctgcgg aactttccgc
     8101 cacgcggcaa tgcagataaa aataacttgc atttgtattt gcaagtaatg aaaatattcc
     8161 gataatatat tattaatcat ccttgttcat tcgcgtttac gctggtcgct tttttaatta
     8221 tgttgcgcga gggtatcgaa gccgcgctca tcgtcggcat cgttgccggt tttttgaaac
     8281 agtccggaca ttcaaaaact gatgcctaag gtctggttcg gggtcgtcct tgcttctttg
     8341 atgtgtttgg gcatcggata cggcatccat tcggcaacgg gcgagattcc ccggaagcag
     8401 caggagttcg tcgtcggcat tatcggtttg gttgccgtcg cgatgctgac ttgtgtggtt
     8461 ttatggatga aaaaggcggc gcgttcgatg aagcggcagc ttcgggattc tgtacaggcg
     8521 gctttgaacc gtggcagcgg tcaaggatgg gccttggtcg gtatggcgtt tcttgccgtg
     8581 gcgcgcgaag gtttggagag cgtttttttc ctgcttgccg tattcaaaca gagcccgacg
     8641 tggcagatgc cggccggcgc ggtagcgggg gttttggctg ccgccgtgat tggcgcgttg
     8701 atttatcagg gcgggatgcg cctgaatctg gcgaagtttt tccgttggac gggggcgttt
     8761 ctgattatcg ttgccgccgg tctgcttgcc ggctcgctgc gtgcgctgca tgaggcaggc
     8821 atttggaacg cgcttcagga cattgtgttg gacccatcaa aatatttgca cgaagacagt
     8881 ccgttgggtg tgctgctcgg cggatttttc ggctataccg accatccggc gcagggctgg
     8941 gccttggttt ggctgctgta ccttattccc gtcatgattt ggtttttgcg cggcagcagg
     9001 ccgtctgaaa ctttaacccg taaagaggaa ttgaaatgag aaaattcaat ttgactgcat
     9061 tgtccgtgat gcttgccttg ggtttgaccg cgtgccagcc gccggaggcg gagaaagccg
     9121 cgccggccgc gtccggtgag acccaatccg ccaacgaagg cggttcggtc ggtatcgccg
     9181 tcaacgacaa tgcctgcgaa ccgatgaatc tgaccgtgcc gagcggacag gttgtgttca
     9241 atattaaaaa caacagcggc cgcaagctcg aatgggaaat cctgaagggc gtgatggtgg
     9301 tggacgaacg cgaaaatatc gccccggggc tttccgacaa aatgaccgta accctgctgc
     9361 cgggggaata cgaaatgacc tgcggccttt tgaccaatcc gcgcggcaag ctggtggtag
     9421 ccgacagcgg ctttaaagac accgccaacg aagcggattt ggaaaaactg ccccaaccgc
     9481 tcgccgacta taaagcctac gttcaaggcg aggttaaaga gctggcggcg aaaaccaaaa
     9541 cctttaccga agccgtcaaa gcaggcgaca ttgaaaaggc gaaatccctg tttgccgaca
     9601 cccgcgtcca ttacgaacgc atcgaaccga ttgccgagct tttcagcgaa ctcgaccccg
     9661 tcatcgatgc gtgtgaagac gacttcaaag acggtgcgaa agatgccggg tttaccggct
     9721 tccaccgtat cgaacacgcc ctttgggtgg aaaaagacgt atccggcgtg aaggaaaccg
     9781 cggccaaact gatgaccgat gtcgaagccc tgcaaaaaga aatcgacgca ttggcgttcc
     9841 ctccgggcaa agtggtcggc ggcgcgtccg aactgattga agaagcggcg ggcagtaaaa
     9901 tcagcggcga agaagaccgt tacagccaca ccgatttgag cgacttccaa gctaatgcgg
     9961 acggatctaa aaaaatcgtc gatttgttcc gtccgttgat tgaggccaaa aacaaagcct
    10021 tgttggaaaa aaccgatacc aacttcaaac aggtcaacga aattctggcg aaataccgca
    10081 ccaaagacgg ttttgaaacc tacgacaagc tgagcgaagc cgaccgcaaa gcattacagg
    10141 ctcctattaa cgcgcttgcc gaagaccttg cccaacttcg cggcatactc ggcttgaaat
    10201 aagccgcaag cgttcagacg gcatttggcg gcagataccg tctgaa
//
LOCUS       DAWTQS010000067         9914 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.067, whole genome shotgun sequence.
ACCESSION   DAWTQS010000067 DAWTQS010000000
VERSION     DAWTQS010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9914
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.067"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            446..1009
                     /locus_tag="WNA25_001759"
     CDS             446..1009
                     /locus_tag="WNA25_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFA9824458.1"
                     /translation="MAIELNGNWTKGFALDHHMQKSILLGYDELGHPHFDSKRSVIGE
                     WVYQLKYRNQTQNVSLLVDYILQKFSGLESINLIVPAPFTTERINQPVQLIAKELSDR
                     LNIPYSPILRKSSSHTPLKNIEEKSEKLEILNNSITIDNIDLSNKNILVIDDLFDSGA
                     TLEISTEKLFSKNARSVIVLAMTKTRG"
     gene            1012..1560
                     /locus_tag="WNA25_001760"
     CDS             1012..1560
                     /locus_tag="WNA25_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824459.1"
                     /translation="MRTIFFSGSRSISRLNPQIRERINNILSNNFDIVIGDANGADKA
                     IQEFLQEQDYANVHIYFSGKIYRNNVGNWQFVQVDSKGTGRVFYTAKDKKMAEIADYG
                     FILWDGKSIGSLNNIAELLQLNKPSLVYHSQTKEFFKIKSSADLENILSNIEDDVLAS
                     ILEKGNTFLKSYVTKQPSLIQE"
     gene            1567..4782
                     /gene="carB"
                     /locus_tag="WNA25_001761"
     CDS             1567..4782
                     /gene="carB"
                     /locus_tag="WNA25_001761"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFA9824460.1"
                     /translation="MPKRTDLKSILIIGAGPIVIGQACEFDYSGAQACKALREEGYKV
                     ILVNSNPATIMTDPEMADVTYIEPIMWQTVEKIIAKERPDAILPTMGGQTALNCALDL
                     ARNGVLAKYNVELIGATEDAIDKAEDRGRFKEAMEKIGLSCPKSFVCHTMNEALAAQE
                     QVGFPTLIRPSFTMGGSGGGIAYNKDEFLAICERGFDASPTHELLIEQSVLGWKEYEM
                     EVVRDKADNCIIICSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIG
                     VDTGGSNVQFAVNPENGEMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGFTLDE
                     LRNDITGGRTPASFEPSIDYVVTKIPRFAFEKFPAADDRLTTQMKSVGEVMAMGRTIQ
                     ESFQKALRGLETGLCGFNPRSEDKAEIRRELANPGPERMLFVADAFRAGFTPEEIHEI
                     CAIDPWFLAQIEDLMKEEKSVSDGQLQDLDYAALRRLKRKGFSDKRLAQLLNVSEKEV
                     REHRYALKLHPVYKRVDTCAAEFATETAYLYSTYEEECESRPSDRKKVMILGGGPNRI
                     GQGIEFDYCCVHAALALRESGFETIMVNCNPETVSTDFDTSDRLYFEPLTLEDVLEIV
                     RTENPWGVIVHYGGQTPLKLANALVENGVNIIGTSADSIDAAEDRERFQKVLNDLGLR
                     QPPNRIAHNEEEALVKAEEIGYPLVVRPSYVLGGRAMQIVHSAEALQKYMREAVQVSE
                     DSPVLLDFFLNNAIEVDVDCVSDGKDVVIGGIMQHVEQAGIHSGDSGCSLPPYSLSEE
                     IQDEIRRQTKAMAYALGVVGLMNVQFAVQDGVVFVLEVNPRASRTVPFVSKATGVPLA
                     KVGARCMAGISLKEQGVEKEVVPDFYAIKEAVFPFIKFPGVDTILSPEMRSTGEVMGV
                     GASFGEAYYKAQLGAGERLNPTGKIFLSVREEDKERVIKTAKNFQALGYGICATRGTA
                     QYLTEHGLIVQAINKVPEGRPHIGDALKNGEIALVVNTVSSDPQSVSDSHIIRQSALQ
                     QRVPQYTTTAGGEAMSEGAKSRDYLGVYSVQELHGRLKNRN"
     gene            complement(4983..6023)
                     /gene="queA"
                     /locus_tag="WNA25_001762"
     CDS             complement(4983..6023)
                     /gene="queA"
                     /locus_tag="WNA25_001762"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFA9824461.1"
                     /translation="MDISDFDFTLPEHLIAQHPPEVRGSSRLLVALPDMPLQDRVFGD
                     LPDYVEAGDVLVFNNTKVMKARLFGQKDSGGRIEALIERVLDNHTALAHIRSSKSPKP
                     GMGLVFEGGIRAVMVGREGELFCLRFEGGQTVYELLEQNGHLPLPPYIERAADADDDS
                     RYQTVYAKYQGAVAAPTAGLHFTEELLRRLKDKGAVTAEVTLHVGAGTFQPVRVDKIE
                     EHKMHSEWFEVPSETVAAVEAAKARGNKAWAVGTTSMRALESAARATGYLKDGQGDTD
                     IFITPGYRFNVVDRLVTNFHLPKSTLLMLVGAFSGMGHIRAVYRHAIEREYRFFSYGD
                     AMVLGRNEGGGL"
     gene            6140..6397
                     /locus_tag="WNA25_001763"
     CDS             6140..6397
                     /locus_tag="WNA25_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HFA9824462.1"
                     /translation="MRKIKLPAHFPQKPPFFDILLDIYRQLRENEHNFTVVSHHRKTV
                     SEHHSARFAPLPQAGCFLKNQRNNPPEHRQPLKEQKWICAN"
     gene            6379..6840
                     /locus_tag="WNA25_001764"
     CDS             6379..6840
                     /locus_tag="WNA25_001764"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFA9824463.1"
                     /translation="MDLRKLKKLIDLVEESGIAEIEVTEGEEKVRITRTIAAAAAPVY
                     AAPVPAAAPAVTPAAAPVAASAPAAAPAARDLSDAQKSPMVGTFYRAPGPNAAAFVEV
                     GQQVKAGDTLCIIEAMKLMNEIEAEKSGTVKEILVENGTPVEFGEPLFIIG"
     gene            6954..8315
                     /gene="accC"
                     /locus_tag="WNA25_001765"
     CDS             6954..8315
                     /gene="accC"
                     /locus_tag="WNA25_001765"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFA9824464.1"
                     /translation="MLKKVLIANRGEIALRVLRACREMGIATVAVHSEADKGSLHVKL
                     ADESVCIGPAASAQSYLNIPAIIAAAEVTFADAVHPGYGFLAENADFAEQVEQSGFTF
                     IGPKPDTIRLMGDKVSAKHAMIAAGVPCVPGSDGALPDDDAEILKIADKVGYPVIIKA
                     SGGGGGRGMRVVEKKEDLLQSVEMTKAEAGAAFGNPMVYMERYLQRPRHVEIQVLADE
                     HGNAVYLAERDCSLQRRHQKVIEEAPAPFIDEEARKKIGKACTDACKRIGYRGAGTFE
                     FLYEDGEFFFIEMNTRVQVEHPVTELITGVDIVQEQLRIASGLPLQYKQKDIKIEGHA
                     FECRINAEDPYNFIPSPGPIESCHLPGGFGIRVDSHIYQGYRIPPYYDSLIGKICVHG
                     KTREQAMAKMRVALAELAVTGIKTNTPLHRDLFADAGFQEGGVSIHYLEHWLEARKTK
                     QDK"
     gene            8419..9306
                     /gene="prmA"
                     /locus_tag="WNA25_001766"
     CDS             8419..9306
                     /gene="prmA"
                     /locus_tag="WNA25_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFA9824465.1"
                     /translation="MPYQQITVNVNDAVAERLADVLMEHGALSAAIEDACAGTQNEQA
                     IFGEPGMPTEQIWQQSKVIALFGEHDEAAAVIDAAAQECGLKDLAYTGETIEDQDWVR
                     LTQSQFDPIRISDRLWITPSWHEAPEGCAVNLRLDPGLAFGTGSHPTTRLCLKWLDTQ
                     LKNGESVLDYGCGSGILTIAALKLGAGSAVGVDIDEQAVRAGRDNAEQNNVDAQFFLP
                     DSLPQGQFDVVVANILANPLRMLGEMLAARTKQGGRIVLSGLLDEQAEELGGIYSQWF
                     DLDPAETDEGWARLSGVKR"
     gene            9324..9887
                     /gene="orn"
                     /locus_tag="WNA25_001767"
     CDS             9324..9887
                     /gene="orn"
                     /locus_tag="WNA25_001767"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFA9824466.1"
                     /translation="MQDKNNLCWLDMEMTGLNPETDRIIEVAMIITDSDLNVLAQSEV
                     YAIHQSDDLLDNMDEWNTATHGRTGLTQRVRESSHTEAEVEQKLLDFMSEWIPGRATP
                     MCGNSIHQDRRFMVKYMPKLENYFHYRNLDVSTLKELAKRWNPPIAKSVVKRGSHKAL
                     DDILESIEEMRHYREHFLISAPKAEAQ"
BASE COUNT         2540 a         2810 c         2521 g         2043 t
ORIGIN      
        1 gagagggcaa caagccgcaa ggcttgtatt tgggcggcga aggtattagg aaagattgcc
       61 accattcgga gaatgccctc cccccagccc tcccccacgg gggagggagt gggtttcggc
      121 agatttttcg gttgccggtg gtttgaaaaa taacttaaat ttgcaaccgt tgatttcagg
      181 tcgtctgaaa aaggatgcac gaaatatcaa cagcgggaat ttttcaggta gcctttattg
      241 tcaagcagat ggaacaaacg ccgcgagcgt tttttcagac aatctttgaa ctcatcggta
      301 gagtgtgtgc cgcaaggcgc gcatgcggtg ggtcggggtt acagggaaaa tgaagaacgc
      361 gtgcgtgcgt accgcacacc ctacatgcgg gctacggctt gctaggattg ctcgttatac
      421 ttaattttga caaggagtca atgaaatggc gatagaacta aatggtaact ggacaaaagg
      481 atttgcttta gaccatcata tgcaaaaaag tattttgtta ggttatgatg aattagggca
      541 tcctcacttt gatagcaaga gaagtgtgat tggagaatgg gtttatcaat taaaatatcg
      601 taatcaaacc caaaatgttt ctttgctggt agactatatt ttacaaaaat ttagtggttt
      661 agaatctata aatttaattg ttcccgcccc ttttacaaca gagcgtatta atcaaccagt
      721 tcaattaatt gccaaagaac taagtgatcg cttgaatatt ccatattctc ctattttaag
      781 aaaatctagc tcacacacac cactcaaaaa tattgaagaa aaatcagaaa aactagaaat
      841 actgaataat tccatcacaa ttgacaatat agatctatcc aataagaata ttttagtaat
      901 cgatgattta tttgatagcg gagcaacatt ggaaatatca actgaaaaat tgttttcaaa
      961 aaatgctaga tctgtaattg ttttagcaat gacaaagact agaggataaa aatgcgcact
     1021 attttctttt ctggttcacg cagcatttca cgactaaatc cacaaatacg agaaagaatt
     1081 aacaatattc ttagcaataa ttttgatatt gttattggag atgctaatgg ggctgataaa
     1141 gctatccaag aatttctaca agaacaagat tatgctaatg tgcatattta cttttctggc
     1201 aaaatttacc gaaataatgt gggaaattgg cagtttgtcc aagtagacag caaaggaact
     1261 ggaagggtat tttatacagc aaaagataag aaaatggcag aaattgctga ttatggcttt
     1321 attttatggg atggtaagag tattggttca ttaaataata tagcagaact tttacagcta
     1381 aataaacctt ctcttgtata ccattctcaa acaaaagaat tctttaaaat taaatcaagc
     1441 gcagatttag agaatatttt atctaacata gaagatgatg ttttagcttc aattttagaa
     1501 aagggaaata cttttcttaa aagctatgta acaaaacaac cttctttaat tcaagagtaa
     1561 ccacccatgc ccaaacgtac cgacctaaaa tccatcctta tcatcggcgc cggccctatc
     1621 gttatcggtc aggcctgcga atttgactat tcgggcgcac aggcctgcaa agccttgcgt
     1681 gaagaaggct ataaagtcat tttggtgaat tccaaccccg ccacgattat gaccgacccc
     1741 gaaatggcgg atgttaccta catcgagccg attatgtggc agacggtgga gaagattatc
     1801 gccaaggagc ggcccgatgc gattctgccc acgatgggcg gtcagaccgc gctgaactgt
     1861 gcgctggatt tggcgcgtaa cggcgtgctg gcgaaataca atgtcgagtt aatcggcgca
     1921 acggaagacg cgatcgacaa ggcggaagac cgcggccgct ttaaagaagc gatggaaaaa
     1981 atcggcctct cttgcccgaa atcttttgtc tgccacacca tgaacgaagc cttggcggcg
     2041 caagaacagg tcggctttcc gacgctgatt cgtccgtctt tcacgatggg cggttcgggc
     2101 ggcggcattg cctacaataa ggatgagttt ttggcgattt gcgaacgcgg tttcgatgcg
     2161 tcgcctacgc atgagctgct gattgagcag tctgtgctcg gctggaaaga gtacgagatg
     2221 gaagtggtgc gcgataaggc ggacaactgc atcatcatct gttcgattga aaacttcgac
     2281 ccgatgggcg ttcatacggg cgactcgatt acggttgcgc cggcgcaaac gctgacggac
     2341 aaggaatacc aaatcatgcg caacgcttcg ttggcggtat tgcgcgaaat cggcgtggac
     2401 acgggcggct cgaacgtgca gtttgcggtg aaccctgaaa acggcgagat gattgtgatc
     2461 gagatgaacc cgcgcgtgag ccgttcgtcc gcgctggctt ccaaagcaac gggcttcccg
     2521 attgcgaagg tggcggcgaa gctggcggtc ggctttacgc tggacgagtt gcgcaacgac
     2581 atcaccggcg gccgcacgcc cgcgtcgttc gagccttcca tcgactatgt ggtaaccaaa
     2641 atcccgcgtt tcgcgtttga aaaattcccc gccgcagacg accgcctgac cacgcagatg
     2701 aaatctgtgg gcgaagtaat ggcgatgggc cgtaccattc aggaaagctt ccaaaaagcc
     2761 ttgcgcggtt tggaaaccgg cttgtgcggt ttcaatccgc gcagcgaaga caaagcggaa
     2821 atccgccgcg aactggccaa ccccggcccc gaacgtatgc tgtttgtggc agacgcgttc
     2881 cgcgcgggct tcacgccgga agaaatccac gagatctgcg ccatcgaccc ttggttcttg
     2941 gcgcaaatcg aagacttgat gaaggaagaa aaatcggttt cagacggcca gttgcaagat
     3001 ttggattacg ccgccctacg ccgtctgaaa cgcaaaggct tctccgacaa acgtttggca
     3061 caattgttga acgtaagcga aaaagaagtt cgcgaacacc gctacgcgct gaagctgcat
     3121 ccggtttaca aacgcgtcga tacctgcgcc gccgagttcg ccaccgaaac cgcctatctt
     3181 tactccactt acgaagaaga atgcgaatcc cgtccttccg accgtaaaaa agtgatgatt
     3241 ctcggcggcg gcccgaaccg catcggtcag ggcatcgagt ttgactactg ctgcgttcac
     3301 gccgcactcg ccctgcgcga atcgggcttt gaaacgatta tggtcaactg caaccccgaa
     3361 accgtgtcca ccgacttcga caccagcgac cgcctctatt tcgagccgct gacattggaa
     3421 gacgtgttgg aaatcgtccg caccgaaaac ccgtggggcg tgattgttca ttacggcggt
     3481 caaaccccgc tgaaactcgc caacgcattg gttgaaaacg gcgtgaacat catcggcaca
     3541 tccgccgaca gcatcgatgc cgccgaagac cgcgaacgct tccaaaaagt gttgaacgac
     3601 ttaggcctgc gccaaccgcc caaccgcatc gcccacaacg aagaagaagc actcgtcaaa
     3661 gccgaagaaa tcggttatcc gctggtcgtg cgcccgtctt atgttttggg cggacgcgcg
     3721 atgcagattg tccactccgc cgaagccttg caaaaataca tgcgcgaagc cgtgcaggtt
     3781 tccgaagaca gccccgtgct gctcgatttc ttcctcaaca acgcgattga agtcgatgtg
     3841 gactgcgttt cagacggcaa agatgtggtt atcggcggca tcatgcagca cgtcgaacag
     3901 gcgggcatcc actccggcga ctccggctgc tcgctgccgc catactcctt aagcgaagaa
     3961 atccaagacg aaatccgccg ccaaaccaaa gcgatggcgt acgcgctggg cgtggtcgga
     4021 ctgatgaacg tgcagtttgc cgtgcaggac ggcgtggtgt tcgtgttgga agtgaacccg
     4081 cgcgccagcc gtaccgtccc ctttgtctcc aaagccaccg gcgtgccgct cgccaaagtc
     4141 ggcgcgcgct gcatggcagg catttccctg aaagaacaag gcgtggaaaa agaagtcgtc
     4201 cccgatttct atgccattaa agaagccgtg ttcccattca tcaaattccc gggcgtggat
     4261 acgattttga gtccggaaat gcgctccacc ggcgaagtga tgggcgtggg cgcaagtttc
     4321 ggcgaagcct actacaaagc ccaactcggc gcgggcgaac gcctcaaccc gaccggcaaa
     4381 atcttcctct ccgtgcgcga agaagacaaa gaacgcgtca ttaaaaccgc caaaaacttc
     4441 caagccttag gctacggcat ctgcgccacg cgcggcacgg cgcaatacct gaccgaacac
     4501 gggctgattg tgcaggccat caacaaagtc cccgaaggcc gcccgcacat cggcgacgcg
     4561 ctgaaaaacg gcgaaatcgc actggtcgta aataccgttt ccagcgatcc gcaatccgtg
     4621 tccgacagcc acatcatccg ccaaagcgca ttgcagcaac gcgtgccgca atacaccacc
     4681 accgccggcg gcgaagcgat gagcgaaggc gcgaaaagcc gggattatct gggtgtgtac
     4741 agcgttcaag agctgcacgg tcgtttgaaa aaccgcaact gatgcctgaa tcaggttgaa
     4801 caatgccgtc tgaagccgtt ttgcggtttc agacggcatt ttgttgcaca tagccttcac
     4861 tttcttttga cctttaagca ccctaatttg gggcggtgga ttatccaact gccggacatc
     4921 cataaaaata ccgcccgaac cacggttcag acggcatctg ccttgcaacg gacagcagca
     4981 gtttaaagcc cgcccccttc gttccgcccc aaaaccatcg catctccgta gctgaagaaa
     5041 cggtattcac gttcaatcgc atgacggtac acggcgcgga tatgacccat acccgaaaac
     5101 gcgccgacca acatcagcag cgtcgatttc ggcagatgaa aattggtaac cagcctgtcg
     5161 acaacattaa aacggtagcc cggcgtgatg aaaatatcgg tgtcgccctg tccgtctttc
     5221 aaatatcccg ttgcgcgcgc ggcagactcg agggcgcgca tggaagtcgt gccgaccgcc
     5281 caggctttgt tcccccgggc ttttgccgcc tcaacggcgg cgacggtttc agacggcact
     5341 tcaaaccatt cgctgtgcat tttgtgttct tcgattttat cgacgcgcac gggttggaat
     5401 gtccccgcac cgacgtgcag ggttacttct gcggttaccg cgcctttgtc tttcagacgg
     5461 cgcaaaagtt cttccgtaaa atgcaggccc gccgtcggcg cggcgaccgc gccctgatat
     5521 ttggcataaa cagtttgata acggctgtcg tcgtccgcat cggcggcacg ttcgatataa
     5581 ggcggcaggg gcaggtgtcc gttctgttcc aaaagttcgt aaacggtttg accgccttca
     5641 aaacgcaggc agaacagttc gccctcacgt ccgaccatca cggcacggat accgccttca
     5701 aacaccagcc ccataccggg cttgggggac ttggacgaac ggatgtgcgc caatgcggta
     5761 tggttgtcca acacacgctc aatcagggct tcgatcctgc cgccgctgtc tttctgccca
     5821 aacagccgcg ccttcatgac tttggtgttg ttgaatacca aaacgtcgcc tgcctcgaca
     5881 taatcgggca aatcgccaaa cacccggtct tgcagcggca tatcgggcag cgcgaccaaa
     5941 agccggctgc tgccgcgcac ctcgggcgga tgctgggcaa tcaggtgttc gggcagggta
     6001 aagtcaaaat ctgaaatatc catttttaca ctctcgttcg ggcaagccgc cattatacgc
     6061 actttagccc tttttcagac ggcatctttg cccgaaaaac caacagatta gaataaacac
     6121 tcttgactcg gggcatcttg tgcgcaaaat caaacttcct gcacatttcc cccaaaaacc
     6181 gccgtttttt gatattttac tggacattta ccgacaactt cgggaaaatg aacacaattt
     6241 cacggtcgtt tcccaccaca ggaaaaccgt atccgaacac cattccgccc ggtttgcgcc
     6301 gttgccgcaa gccggctgtt ttctgaaaaa ccaacgcaac aacccgccgg aacaccggca
     6361 gcctttaaag gaacagaaat ggatttgcgc aaattaaaaa aactgattga tttggttgaa
     6421 gaatcgggta tcgccgaaat cgaagtaacc gaaggcgagg aaaaagtccg cattacccgt
     6481 accattgcag ctgcggcagc tcccgtttac gccgcgcccg tacctgccgc cgcgccggcc
     6541 gtaacgccgg ccgccgcacc cgttgcggca tccgcgcccg ccgccgcgcc ggccgcccgc
     6601 gatttgtccg acgctcaaaa atcgcctatg gtcggcacgt tctaccgcgc gcccggcccg
     6661 aatgccgccg catttgtcga agtcggtcaa caggtcaaag ccggcgacac gctgtgcatc
     6721 atcgaagcga tgaagctgat gaacgaaatc gaagccgaaa aatccggcac ggtcaaagaa
     6781 attctggtcg aaaacggtac gcccgtcgaa ttcggcgaac cgctcttcat catcggataa
     6841 tcctgttttc agacggcata aacttccgat gccgtctgaa atgctttccc ccccttcagc
     6901 gttcccgcac ccttttttac ggacgggttg ccggaaccgc aggaaaggtc atcatgctga
     6961 aaaaagtttt aatcgccaac cggggcgaaa tcgccttgcg cgtactccgt gcctgccgcg
     7021 aaatgggcat tgccaccgtt gccgtgcatt ccgaagccga caaaggcagc ctgcacgtca
     7081 aactcgccga cgaatccgtg tgcatcggtc ctgccgcttc tgcgcaaagt tatctgaaca
     7141 ttcccgccat cattgccgcc gccgaagtaa ccttcgcgga cgctgtccac ccgggctacg
     7201 gcttccttgc cgaaaacgcc gatttcgccg aacaggtcga acagtccggc tttaccttca
     7261 tcggcccgaa acccgacacc atccgcctga tgggcgacaa agtctccgcc aaacacgcga
     7321 tgatagcggc aggcgtgccc tgcgtccccg gctctgacgg cgcattgccc gacgatgatg
     7381 ccgaaatcct caaaatcgcc gacaaagtcg gttatcccgt gattatcaaa gcatcgggcg
     7441 gcggcggcgg gcgcggtatg cgcgtggtcg agaaaaaaga agacctcctc cagtctgtcg
     7501 aaatgaccaa ggctgaagcg ggcgcggcgt tcggcaaccc gatggtttat atggaacgct
     7561 acctgcaacg cccgcgccac gtcgaaatcc aagtgcttgc cgacgaacac ggcaacgccg
     7621 tctatcttgc cgagcgcgac tgttcgctgc aacgccgcca ccagaaagtc atcgaggaag
     7681 cacccgcccc gtttatcgat gaagaggcac gcaaaaaaat cggcaaagcc tgtaccgacg
     7741 cgtgcaaacg catcggctac cggggcgcgg gcacgtttga atttttatac gaagacggcg
     7801 aatttttctt tatcgagatg aacacgcgcg ttcaggtcga gcatccggtt accgaactca
     7861 ttaccggcgt ggacatcgtc caagagcaac tccgcatcgc atccggcctg ccgctgcaat
     7921 acaaacagaa agacatcaaa atcgaaggcc acgccttcga gtgccgcatc aatgccgaag
     7981 acccgtacaa cttcatcccc agcccgggcc cgattgaaag ctgccacctg cccggcggct
     8041 tcggcatccg cgtggacagc cacatctacc aaggctaccg catcccgccg tactacgaca
     8101 gcctgatcgg caaaatctgc gtacacggca aaacgcgtga acaggcaatg gcgaaaatgc
     8161 gcgtcgcact cgccgagctg gcggtaaccg gcatcaaaac caatacgccg ttgcaccgcg
     8221 acctgttcgc cgatgcgggt ttccaagaag gcggagtgag catccattac ctggagcact
     8281 ggctggaagc gcgcaaaacc aaacaggaca aataagccgc cgccgatatg ccgtctgaag
     8341 ccgcccttcc gcgttcagac ggcatttccc ttgccccgcc gtctgaaacc gatttcgata
     8401 tttccaagaa agcccgttat gccctaccaa caaatcaccg tcaacgtcaa cgatgccgtc
     8461 gccgaacgcc tcgccgacgt gctgatggaa cacggtgcac tctccgccgc catcgaagat
     8521 gcctgcgccg gcacgcaaaa cgaacaggcg attttcggcg aacccggtat gcccaccgaa
     8581 caaatctggc agcagagcaa agtcatcgcc ctgttcggcg aacacgacga agccgccgcc
     8641 gtcatcgatg ccgccgcaca agaatgcggg ttaaaagact tggcatacac cggcgaaacc
     8701 atcgaagacc aagactgggt gcgcctcacg caatcgcaat tcgaccccat ccgaatttcc
     8761 gaccgcctgt ggattacccc ctcttggcac gaagcccccg aaggctgcgc cgtcaacctc
     8821 cgcctcgatc ccggactcgc cttcggcacc ggcagccacc cgaccacgcg cctctgcctc
     8881 aaatggctgg atacgcaact caaaaacggc gaaagcgtcc tcgactacgg ctgcggttcg
     8941 ggcatcctga ccatcgccgc cctcaaactc ggtgcaggtt ccgccgtcgg cgtggatatt
     9001 gacgaacagg ccgtccgcgc cggcagggac aacgccgaac agaacaacgt cgatgcacaa
     9061 ttcttcctgc ccgacagtct gcctcaaggg caattcgatg tcgtcgtcgc caacatcctc
     9121 gccaaccctt tgcgtatgct cggcgaaatg cttgcagccc gcaccaaaca gggcggacgc
     9181 atcgtgttgt ccggtttgtt ggatgaacag gccgaagaac tcggcggcat ttacagccaa
     9241 tggttcgatc tcgacccggc ggaaactgac gaaggctggg cgcgcttgag cggcgtaaaa
     9301 cgctgaaacg gaaaggaaac accgtgcagg acaaaaacaa cctctgctgg ctcgatatgg
     9361 aaatgacggg gctgaatccc gaaaccgacc gcattatcga agtcgcgatg attattaccg
     9421 actcggattt gaatgtgttg gcgcaatccg aagtttacgc catccaccaa agcgacgatc
     9481 tgctcgacaa tatggacgag tggaacaccg ccacacacgg caggacgggg ctgacgcagc
     9541 gcgtgcgcga atcgtcgcat accgaagccg aagtcgaaca gaaactgctg gactttatgt
     9601 cggaatggat acccggacgc gccacaccga tgtgcggcaa ctccatccac caagaccggc
     9661 gttttatggt caaatatatg ccgaaactgg aaaactactt ccactaccgc aacctcgacg
     9721 tttccacgct gaaagaactc gccaaacgct ggaatccgcc cattgccaaa agcgtcgtca
     9781 aacgcggttc gcacaaggca ttggacgaca ttttggagag catcgaagaa atgcgccact
     9841 accgcgaaca cttcctgatt tccgccccga aagccgaagc gcaataagaa acaaacaatg
     9901 ccgtctgaaa cgct
//
LOCUS       DAWTQS010000068         9859 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.068, whole genome shotgun sequence.
ACCESSION   DAWTQS010000068 DAWTQS010000000
VERSION     DAWTQS010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9859
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.068"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            120..1385
                     /gene="efeB"
                     /locus_tag="WNA25_001768"
     CDS             120..1385
                     /gene="efeB"
                     /locus_tag="WNA25_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HFA9824467.1"
                     /translation="MSQNQPAQPTKRNLFKTALAVGAIGAIGGYFGGKKQGETAERTA
                     ESQHSPQAYPCYGEHQAGIVTPRQAFSIMCAFDVTAQSAKQLENLFRTLTARIEFLTQ
                     GGEYQDGDDKLPSAGSGILGKAFNPDGLTVTVGVGSSLFDGRFGLKDKKTVHLQEMRD
                     FPNDKLQKSWCDGDLSLQICAFTPETCQAALRDIIKHTAQTAVIRWSIDGWQPKSEPG
                     AMAARNLLGFRDGTGNPKVSDPKTADEVLWTGVAANSLDEPEWAKNGSYQAVRLIRRF
                     VEFWDRTPLQEQTDIFGRRKYSGAPMDGKKEADQPDFAKDPEGNTTPKDSHMRLANPR
                     DPEFLKKHRLFRRAYNYSRGPASSGQLDVGLVFVCYQANLADGFIFVQNLLNGEPLEE
                     YISPFGGGYFFVLPGVGKGGFLGQGLPGV"
     gene            complement(1457..1786)
                     /locus_tag="WNA25_001769"
     CDS             complement(1457..1786)
                     /locus_tag="WNA25_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HFA9824468.1"
                     /translation="MSLPPCPQCASEYTYEDGGQYICPECAHEWNETESAADLAAQVR
                     DANGAALQNGDTVILIKDLKVKGSSMTIKQGTKVKGIRLQEGDHNIGCKIDGSAMNLK
                     SEFVKKA"
     gene            complement(1861..3231)
                     /gene="glmU"
                     /locus_tag="WNA25_001770"
     CDS             complement(1861..3231)
                     /gene="glmU"
                     /locus_tag="WNA25_001770"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFA9824469.1"
                     /translation="MPQNTLNTVILAAGKGTRMYSQMPKVLHCIGGKPMVERVIDTAA
                     ALNPQNICVVVGHGKEQVLDTVKRDAVWVEQTEQLGTGHAVKTALPHLASEGRTLVLY
                     GDVPLIDVETLETLLEAAGNEVGLLTDVPADPAGLGRIIRDGSGSVTAIVEEKDASAT
                     QKTIREINTGILVLPNAKLENWLNSLSSNNAQGEYYLTDLIAKAVADGIKVRPVRVRA
                     SHLAAGVNNKRQLAELERIFQTEQAQELLKAGVTLRDPARFDLRGRLKHGQDVVIDVN
                     VVIEGEVELGDNVEIGANCVIKNAKIGANSKIAPFSHLEGCEVGENNRIGPYARLRPQ
                     AKLADNVHVGNFVEIKNAAIGKGTKANHLTYIGDAEVGSKTNFGAGTIIANYDGVHKH
                     KTVIGDEVRIGSNCVLVAPVTLGNKVTTGAGSAITRNIEDNKLALARARQTVIEGWVR
                     PEKDKQ"
     gene            complement(3286..3558)
                     /locus_tag="WNA25_001771"
     CDS             complement(3286..3558)
                     /locus_tag="WNA25_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex protein"
                     /protein_id="HFA9824470.1"
                     /translation="MRKTFLILTVSAALLSGCAWETYQDGNGKTAVRQKYPAGTPVYY
                     QDGSYSKNMNYNQYRPERRAVLPDQTGNNADEEHRQHWQKPKFQNR"
     gene            complement(3551..4264)
                     /locus_tag="WNA25_001772"
     CDS             complement(3551..4264)
                     /locus_tag="WNA25_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFA9824471.1"
                     /translation="MQENPTVWLFDLDNTLHDADAGIFHLINRAMTRYMARRLKLSES
                     AASDLRQDYWHRYGATLAGLQIHHPEIDIAEFLRESHPIDAILTRLHGMADTENTLCR
                     LNGRKAVFSNGPSFYVRAVAGALGLENCFDALFGTDDFGLLYKPNPQAYLNVCRLLDV
                     PPECCIMVDDSADNLHQAKALGMKTVRFGAKSHTPPFIDASVSDMAQLARYAETLSER
                     RQNHYNTRTPPENTKECYA"
     gene            4323..5324
                     /locus_tag="WNA25_001773"
     CDS             4323..5324
                     /locus_tag="WNA25_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFA9824472.1"
                     /translation="MKRKIWLLPLLAVSAYLQAQTEVRLAVHKSFSLPKGLIARFERA
                     NDAKVSIIQAGGANEMLNKLILSRANPIADAVYGLDNANIGKAREMGILAAAQPESAP
                     VAVGLPSALAVDYGYVSVNYDKKWFEGKKLPLPQTLQDLTRPEYKNLLVVPSPATSSP
                     GLGFLMANIGGLGEEGAFKWWAQMRQNGVKVAKGWSEAYYTDFSHNGGAYPLVVGYAA
                     SPAAEVYFSKGKYSEPPTGNLFLKGGVFRQVEGAAVLKGAKQPKLAAKLVQWLQSPEV
                     QQAVPSEMWVYPAVKNTRLPDVFRFAQAPAHTTAPAQRDIDANQRGWVSRWIRTVLK"
     gene            5418..6266
                     /locus_tag="WNA25_001774"
     CDS             5418..6266
                     /locus_tag="WNA25_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFA9824473.1"
                     /translation="MDFKQFDFLHLISVSGWGHLAEKAWAFGLNLAAALLIFLVGKWA
                     AKRIVAVMRAAMTRAQVDATLISFLCNVANIGLLILVIIAALGRLGVSTTSVTALIGG
                     AGLAVALSLKDQLSNFAAGALIILFRPFKVGDFIRVGGFEGYVREIKMVQTSLRTTDN
                     EEVVLPNSVVMGNSIVNRSSLPLCRAQVIVGVDYNCDLKVAKEAVLKAAAEHPLSVQN
                     EERQPAAYITALGDNAIEITLWAWANEADRWTLQCDLNEQVVENLRKVNINIPFPQRD
                     IHIINS"
     gene            6292..6774
                     /locus_tag="WNA25_001775"
     CDS             6292..6774
                     /locus_tag="WNA25_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HFA9824474.1"
                     /translation="MDALHTIARNLTKKRQTVSCAESCTGGMLAAAFTSVAGSSQWFD
                     QSFVTYSNKAKEYRLGVLPETLLEHGAVSRQTVYEMARGAKAVAQADYAVGISGIAGP
                     GGGESKPVGTVWFGFAFPGGSCEAMRRFDGNRESVRAQAVAFALERLAGLVENGGDAV
                     "
     gene            complement(6858..8426)
                     /gene="msrAB"
                     /locus_tag="WNA25_001776"
     CDS             complement(6858..8426)
                     /gene="msrAB"
                     /locus_tag="WNA25_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional peptide-methionine (S)-S-oxide
                     reductase MsrA/peptide-methionine (R)-S-oxide reductase
                     MsrB"
                     /protein_id="HFA9824475.1"
                     /translation="MKHRTFFSLCAKFGCLLALGACSPKIVDAGAATVPHTLSTLKTA
                     DNRPASVYLKKDKPTLIKFWASWCPLCLSELGQAEKWAQDAKFSSANLITVASPGFLH
                     EKKDGEFQKWYAGLNYPKLPVVTDNGGTIAQNLNISVYPSWALIGKDGDVQRIVKGSI
                     NEAQALALIRNPNADLGSLKHSFYKPDTQKKDSAIMNTRTIYLAGGCFWGLEAYFQRI
                     DGVVDAVSGYANGNTENPSYEDVSYRHTGHAETVKVTYDADKLSLDDILQYYFRVVDP
                     TSLNKQGNDTGTQYRSGVYYTDPAEKAVIAAALKREQQKYQLPLVVENEPLKNFYDAE
                     EYHQDYLIKNPNGYCHIDIRKADEPLPGKTKAAPQGKGFDAATYKKPSDAELKRTLTE
                     EQYQVTQNSATEYAFSHEYDHLFKPGIYVDVVSGEPLFSSADKYDSGCGWPSFTRPID
                     AKSVTEHDDFSFNMRRTEVRSRAADSHLGHVFPDGPRDKGGLRYCINGASLKFIPLEQ
                     MDAAGYGALKGEVK"
     gene            8572..9825
                     /gene="ftsY"
                     /locus_tag="WNA25_001777"
     CDS             8572..9825
                     /gene="ftsY"
                     /locus_tag="WNA25_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFA9824476.1"
                     /translation="MFSFFRRKKKQETPALEEAQVQETAAKVESEVAQIVGNIKEDVE
                     SLAESVKGRAESAVETVSGAVEQVKETVAEMPSEAGEAAERVESAKEAVAETVGEAVG
                     QVQEAVATTEEHKLGWAARLKQGLAKSRDKMAKSLAGVFGGGQIGEDLYEELETVLIT
                     GDMGMEATEYLMKDVRDRVSLKGLKDGNELRGALKEALYDLIKPLEKPLVLPETKEPF
                     VIMLAGINGAGKTTSIGKLAKYFQAQGKSVLLAAGDTFRAAAREQLQAWGGRNNVTVI
                     SQTAGDSAAVCFDAVQAAKARGIDIVLADTAGRLPTQLHLMEEIKKVKRVLQKAIPGA
                     PHEIIVVLDANIGQNAVNQVKAFDDALGLTGLIVTKLDGTAKGGILAALASDRPVPVR
                     YIGVGEGIDDLRPFDARAFVDALLD"
BASE COUNT         2036 a         2603 c         2992 g         2228 t
ORIGIN      
        1 gttaatccgc tatatccgcc atatgttgca gggcgggctt tcagaccgcc gctatcggtt
       61 aatggaaaaa cggcgtgcag ggatgcccac cctgctgcac ggatattgaa ggaaacgcca
      121 tgagtcaaaa ccaacccgca caaccgacca aacgcaatct gttcaaaacc gcccttgccg
      181 tcggcgcaat cggcgcaatc ggaggttatt tcggcggcaa aaaacagggc gaaaccgccg
      241 aacgcaccgc cgaaagccaa cactcgcccc aagcctatcc ctgctacggc gaacatcagg
      301 caggtatcgt tacgccgcgg caggcgtttt ccattatgtg cgccttcgac gtaaccgcgc
      361 aaagtgccaa gcagctggaa aacctgttcc gcacactgac cgcccgcatc gagtttctca
      421 cccaaggcgg agaataccaa gacggcgacg acaaactccc gtcagccggc agcggcattt
      481 tgggcaaagc cttcaacccc gacggattga ccgttaccgt gggggtgggc agcagcctgt
      541 ttgacggccg gttcggactc aaagacaaaa aaacggttca tttgcaggaa atgcgcgact
      601 tccccaacga taagctgcaa aaaagctggt gcgacggcga tttgagcctg caaatctgcg
      661 ccttcacccc cgaaacctgc caagccgccc tgcgcgacat catcaaacac accgcccaaa
      721 ccgccgtcat ccgctggagt atcgacgggt ggcagcctaa atccgaaccc ggcgcgatgg
      781 cggcgcgcaa cctgttgggc ttccgagacg gcacgggcaa ccccaaggtt tccgatccca
      841 aaaccgccga cgaggtttta tggacgggcg tggccgccaa cagcctcgac gaaccggagt
      901 gggcgaaaaa cggcagctat caggcagtcc gccttatccg ccgctttgtc gagttttggg
      961 acaggacgcc gcttcaagag caaaccgaca ttttcgggcg gcgaaaatac agcggggcgc
     1021 cgatggacgg caaaaaagaa gccgaccaac cggatttcgc caaagacccc gagggtaata
     1081 ccacgcccaa agacagccat atgcgcctgg cgaatccgcg cgatcccgaa ttcctcaaaa
     1141 aacaccgcct cttccgccgc gcctacaact attctcgcgg acccgcctca agcggacagc
     1201 ttgatgtcgg gctggtgttc gtctgctatc aggcaaatct tgccgacggt ttcatcttcg
     1261 tgcaaaacct cctcaacggc gaaccgctgg aagaatacat cagccccttc ggcggcggct
     1321 atttcttcgt cttgcccggc gtgggaaaag gcggattctt gggacaaggg ctgccgggcg
     1381 tataaacctg cctgacggac aaatgccgtc cgaaccttgc caaacgggtt cggacgggat
     1441 tttactgttt ggacggtcag gcttttttga cgaattcgga ttttaaattc atcgcgctgc
     1501 cgtcgatttt gcagccgatg ttgtgatcgc cttcctgcag gcgtatgcct ttgaccttcg
     1561 taccttgttt gatcgtcatc gagctgcctt ttaccttgag gtctttgatg aggatgacgg
     1621 tgtcgccgtt ttgcagcgcc gcgccgttgg catcgcgcac ttgagccgca aggtcggcgg
     1681 cggattcggt ttcattccat tcgtgggcgc attcggggca gatgtattgt ccgccgtctt
     1741 cataggtgta ttcggaggcg cattgcgggc aagggggtaa tgacatggtt tgccgtcctt
     1801 atcggatgtt tgttttggga tgccgtctga aacctgaaac cggcttcaga cggcacggct
     1861 ttattgtttg tctttttccg gccttaccca gccttcgatg acggtttggc gggcgcgggc
     1921 gagggcgagt ttgttgtctt cgatattgcg ggtaatcgcg ctgcctgcgc ctgtggttac
     1981 tttgttgccc agtgttacag gggcaaccag tacgcagttc gaaccgatgc gcacttcgtc
     2041 gccgatgacg gttttgtgtt tgtgcacgcc gtcgtagttg gcaataatcg tgcccgcgcc
     2101 gaagttggtt ttgctgccga cttcggcatc gcctatgtag gtgaggtggt tggctttggt
     2161 gcctttgccg atggcggcgt ttttgatttc gacgaagttg ccgacgtgta cattgtcagc
     2221 cagtttggct tgcggacgca aacgggcgta cgggccgatt cggttgtttt cgccgacttc
     2281 gcagccttcg aggtgggaga agggggcgat tttgctgttt gcgccgattt tggcgttttt
     2341 gatgacgcag tttgcgccga tttcgacgtt gtcgccgagt tcgacttcac cttcgatgac
     2401 gacgttgacg tcaatcacga catcctgtcc gtgtttcaga cggccccgca aatcgaaacg
     2461 cgccggatcg cgcagggtca cgcctgcttt gagcaattct tgcgcctgtt cggtttggaa
     2521 gatgcgttcg agttcggcga gctggcgttt gttgttcacg ccggcggcga ggtgggaggc
     2581 gcgcacccgg acggggcgga ctttgatgcc gtcggcaacg gctttggcga tgaggtcggt
     2641 taaataatat tcgccttggg cattgttgct ggaaaggctg ttcagccagt tttccagttt
     2701 ggcgttgggc aggacgagga tgcctgtgtt gatttcgcgg atggtttttt gggtggcact
     2761 tgcgtctttt tcttcgacaa tggcggttac gctgccgctg ccgtcgcgga tgatgcgccc
     2821 caagcctgcc gggtcggcgg gcacgtcggt caacagcccg acttcgttgc ctgcggcttc
     2881 gagcagggtt tcgagggttt caacgtcaat taaaggaacg tcgccgtaca acaccagcgt
     2941 gcggccttcg gaggcaaggt ggggcagggc ggttttgacg gcgtggccgg tgccgagttg
     3001 ttcggtttgt tcgacccaga cggcatcgcg tttgacagtg tctaaaactt gctctttgcc
     3061 gtgtccgacg acgacgcaga tgttttgcgg attcagcgcg gcggcggtgt cgataacgcg
     3121 ctcgaccatg ggcttgccgc cgatgcagtg cagcactttg ggcatttggg aatacatgcg
     3181 cgtgcctttg ccggcggcga ggatgacggt gtttaaagtg ttttgcggca tagcgttttc
     3241 ctgtgccatg ccgtctgaag cggtttcaga cggcataggg taggtttatc ggttttgaaa
     3301 ctttggtttt tgccagtgtt ggcgatgctc ttcgtcggcg ttgttgccgg tttggtcggg
     3361 cagcacggcg cggcgttcgg gacggtattg gttgtagttc atatttttcg agtagctgcc
     3421 gtcttggtaa taaacgggcg tgccggcggg atatttttga cggacggcgg ttttgccgtt
     3481 gccgtcttga taagtttccc acgcgcagcc cgacaaaagg gcggcggaga cggtcaggat
     3541 aaggaaggtt ttacgcatag cattctttcg tattttcggg gggggtgcgg gtattgtaat
     3601 gattttggcg gcgttctgac aaagtttctg cataccgtgc cagttgcgcc atatcgctta
     3661 cggaggcatc gataaagggc ggcgtgtggg attttgcacc gaaccggacg gttttcatgc
     3721 ccaatgcttt tgcctgatgg aggttgtccg cgctgtcgtc caccataatg cagcattcgg
     3781 gcggtacgtc caacaggcgg cagacattga gatacgcttg cggattgggt ttgtacagca
     3841 gtccgaaatc atccgtgccg aacagtgcgt cgaaacagtt ttccaaaccg agtgcgccgg
     3901 caacggcacg gacgtaaaac gacgggccgt tggaaaaaac cgccttgcgc ccgttcagac
     3961 ggcatagggt gttttccgta tccgccatgc cgtgcagcct ggtcaggatt gcatcgatcg
     4021 gatggctttc gcgtaaaaat tccgcaatgt cgatttcggg atggtggatt tgcagtccgg
     4081 cgagcgttgc gccgtaacgg tgccaatagt cttgacgcag gtcggacgcg gcggattcgg
     4141 agagtttgag gcggcgtgcc atatagcgcg tcatagcgcg gttgatgagg tggaagatgc
     4201 ctgcgtcggc atcgtgcagc gtgttgtcga ggtcgaacag ccacacggtc gggttttctt
     4261 gcattttgaa ccgtgaaaat ttgttagaat gttattttac agcgaataaa ggaagactcg
     4321 gaatgaaacg gaaaatttgg ctgctgccgc tgctggcggt ttcggcatac ctgcaggcgc
     4381 agacggaagt caggctggcg gtgcataagt cgttcagcct gcccaaaggg ctgattgcgc
     4441 gcttcgagcg ggcaaacgat gcgaaagtgt cgattattca ggcgggcggc gcgaacgaaa
     4501 tgctcaacaa actgattttg agccgagcca acccgattgc cgacgcggtg tacggtttgg
     4561 acaacgccaa tatcggcaag gcgcgggaaa tgggcatttt ggcggcggcg caacccgaat
     4621 ccgcccccgt cgcggtcggg ctgccttcgg ctttggcggt cgattacggc tatgtgtccg
     4681 tcaattacga caaaaaatgg tttgaaggca aaaagctgcc cctgccgcaa accctgcagg
     4741 atttgacccg ccccgaatat aaaaacctat tggtcgtgcc gtcccccgcc acatcttccc
     4801 ccggcttggg cttcctgatg gcgaacatcg gcggtctggg cgaagaaggc gcgtttaaat
     4861 ggtgggcaca gatgcggcag aacggcgtga aggtcgccaa aggctggagc gaggcgtatt
     4921 acaccgactt ttcgcacaac ggcggcgcgt atccgctggt ggtcggttat gccgccagcc
     4981 ctgcggcgga agtatatttt tccaaaggca aatacagcga gccgccgacg ggcaacctgt
     5041 ttttaaaagg cggcgtattc cgtcaggtcg aaggcgcggc ggtcttgaag ggcgcgaaac
     5101 agccgaagct ggcggcaaaa ctggtgcaat ggctgcaaag tccggaagtg cagcaggcgg
     5161 ttccgtctga aatgtgggtt taccccgccg tcaaaaacac gcgcctgccc gacgtgttcc
     5221 gcttcgccca agccccggcg cacactaccg cccccgcgca gcgcgatatt gatgcgaacc
     5281 agcgcggatg ggtttcccgt tggatcagga cggttttgaa ataaaacaaa cataccgccc
     5341 cgcagggctt cagacggcat ttttacacct gtgccgatta cgccgcacgg ggcggatgtt
     5401 cgatcgagag gaaaacaatg gacttcaaac aatttgattt tttacacctg atcagtgttt
     5461 ccggttgggg gcatctggct gaaaaggcgt gggcgttcgg gctgaacctt gccgccgcgc
     5521 tgcttatttt cttggtcggg aaatgggcgg cgaaacgcat tgtcgccgtg atgagggcgg
     5581 cgatgacgcg cgcgcaggtc gatgccacgc tgattagttt tttgtgtaat gttgccaata
     5641 tcggcttatt gattttggtg attattgccg cattgggcag attgggcgtt tccacaacat
     5701 ccgtaaccgc cttaatcggc ggcgcgggtt tggcggtggc gttgtcctta aaagaccagc
     5761 tgtccaattt tgccgccggc gcgctgatta tcctgttccg cccgttcaaa gtcggcgact
     5821 ttatccgtgt cggcggtttt gaaggatatg tccgggaaat caaaatggtg cagacttctt
     5881 tgcggacgac cgacaacgaa gaagtcgtgc tgcccaacag cgtggtgatg ggcaacagca
     5941 tcgtcaaccg ttccagcctg ccgctttgcc gcgcccaagt gatagtcggc gtcgattaca
     6001 actgcgattt gaaagtggcg aaagaggcgg tgttgaaagc cgccgccgaa caccccttga
     6061 gcgttcaaaa cgaagagcgg cagcccgccg cctacatcac cgccttgggc gacaatgcca
     6121 tcgaaatcac attatgggct tgggcaaacg aagcagaccg ctggacgctg caatgcgact
     6181 tgaacgaaca agtggtcgaa aacctccgca aagtcaatat caacatcccg ttcccgcaac
     6241 gcgacataca catcatcaat tcttaaacgc cgtctgaaag gggggtggga aatggacgcg
     6301 ttgcacacca tcgcccgaaa cctgacgaaa aaacgtcaaa ccgtaagctg tgccgaatcc
     6361 tgcacgggcg gaatgcttgc cgccgcattc acaagcgttg caggcagttc gcaatggttc
     6421 gaccagagtt ttgtaacata cagcaacaaa gccaaagaat accgcttggg cgtgttgccc
     6481 gaaaccctgc tcgaacacgg cgcggtcagc cgccaaaccg tctatgagat ggcgcgcggc
     6541 gcgaaagccg tggctcaggc ggattacgcc gtcggtattt ccggcatcgc cggcccgggc
     6601 ggcggcgaaa gcaaacccgt cggcacggtt tggttcgggt ttgcctttcc gggcggaagt
     6661 tgcgaagcaa tgcgccgttt tgacggcaac cgcgaatccg tccgcgcgca ggcggtcgcc
     6721 ttcgcgttgg aacggttggc cgggctggtt gaaaacggcg gcgatgctgt ctgaacaaat
     6781 ccccgccgga taaaaaatgc cgtctgaaac gttgcaggtt tcagacggca ttttgccggg
     6841 ttaggcggcg gtgcggctta tttcacttcg cccttcaacg cgccgtagcc tgccgcgtcc
     6901 atttgttcca gcgggatgaa tttcaagctc gcgccgttga tgcagtagcg cagtccgcct
     6961 ttgtcgcggg ggccgtcggg gaagacgtgt cccaagtgcg aatcggcggc gcggcttctg
     7021 acttcggtgc ggcgcatatt gaagctgaaa tcatcgtgtt cggtaacgga ttttgcatca
     7081 atcgggcgcg tgaagctcgg ccagccgcag ccggaatcat atttgtcggc ggagctgaac
     7141 aggggttcgc cgctgacaac gtccacataa atgccgggtt tgaacaaatg gtcgtattcg
     7201 tggctgaagg cgtattcggt cgcgctgttt tgggtcactt ggtattgctc ttcggtcagg
     7261 gtgcgtttga gttcggcgtc actcggtttt ttatacgttg ccgcgtcgaa gcctttccct
     7321 tgcggtgcgg ctttggtttt gcccggcagc ggttcgtcgg ctttgcggat gtcgatgtgg
     7381 cagtagccgt tggggttttt aatcaggtag tcctgatggt attcctcggc gtcgtagaag
     7441 tttttcagcg gctcgttttc aacaacgagg ggcagttggt atttttgctg ctcgcgtttg
     7501 agggcggcgg cgatgacggc tttttcggcg gggtcggtgt agtacacgcc gctgcggtat
     7561 tgcgtgccgg tgtcgttacc ctgtttgttg aggctggtcg gatcaacgac gcggaaataa
     7621 tattgcagga tgtcgtccag gctgagtttg tcggcatcgt aggtcacttt gacggtctcg
     7681 gcatggcccg tatggcggta ggacacgtct tcgtagctcg ggttttccgt gttgccgttg
     7741 gcgtagccgg ataccgcgtc aaccacgccg tcgatgcgtt ggaaataggc ttccaagccc
     7801 cagaagcagc cgccggcgag gtagatggtg cgcgtgttca tgattgctga atcctttttc
     7861 tgagtgtcgg gtttgtagaa cgaatgtttc aaactgccca aatcggcatt cgggttgcgg
     7921 attaacgcca atgcctgcgc ttcgttgatg ctgcctttga cgatgcgctg cacgtcgccg
     7981 tctttaccga ttaacgccca agaaggataa acgctgatat tcaggttttg ggcgatcgtg
     8041 ccgccgttgt cggtaacgac gggcagcttg gggtagttca aaccggcata ccatttttga
     8101 aactcgccgt cttttttctc gtgcaaaaag ccgggggagg cgacggtaat caggttggcg
     8161 gagctgaatt ttgcatcttg cgcccatttc tcggcctgtc ccaattcgga caaacataaa
     8221 ggacaccagc tcgcccaaaa tttaatcagc gtcggtttgt cttttttcaa ataaacactg
     8281 gcggggcggt tgtccgcggt ttttaacgtg gataaagtgt gcggcacggt cgcggccccg
     8341 gcatcgacga ttttgggcga acaagcgccc agcgcaagca ggcagccgaa cttggcgcaa
     8401 agggaaaaga aagtacggtg tttcattttg atgtttcctg tgtggacggt ttgcatgatt
     8461 agacgtttga gatgccgaaa ccttacggcc cggattttca gacaaccttg ccgcgcaaaa
     8521 tacgctacaa tacgccctat ttcaagtttc taaaattaaa aggaaaattc aatgttcagc
     8581 ttcttccgtc gcaagaaaaa acaggaaacg ccggctctcg aggaggccca agttcaggaa
     8641 accgcagcaa aagtagaatc tgaagttgct caaatagttg gaaatattaa agaagatgtc
     8701 gaatctttag cagaaagcgt caaagggcgg gccgaatctg ccgttgaaac cgtcagcggt
     8761 gcggttgaac aggtaaagga aaccgttgcc gagatgccgt ctgaagcagg ggaagcggcg
     8821 gaacgcgttg aatccgcaaa agaagctgtt gccgaaaccg tcggcgaggc tgtcgggcaa
     8881 gttcaagaag ccgttgcgac aactgaagaa cacaagctcg gttgggctgc gcgtttgaaa
     8941 caaggcttgg ccaaatcacg cgacaaaatg gcgaaatcgc tggccggcgt gttcggcggc
     9001 ggacaaatcg gcgaggattt gtacgaagag ctggaaaccg tgctgattac cggcgatatg
     9061 ggcatggagg ccaccgaata cctgatgaaa gacgtgcgcg accgcgtcag cctcaaaggg
     9121 ctgaaagacg gcaacgaatt gcgcggcgcg ttgaaagaag ccttgtacga cctgattaag
     9181 ccgttggaaa aaccgctggt cttgcccgaa actaaagagc ctttcgtgat tatgcttgcc
     9241 ggtatcaacg gcgcgggcaa aaccacgtct atcggcaaac tcgccaaata tttccaagcg
     9301 cagggcaaat ccgtattgct ggcggcaggc gatactttcc gcgccgccgc gcgcgagcag
     9361 cttcaggctt ggggcgggcg caacaatgtg accgtgattt cacaaactgc gggcgattcc
     9421 gccgccgtgt gcttcgatgc cgtccaagcc gcgaaagcgc gcgggatcga catcgtgctt
     9481 gccgacaccg ccggccgcct gcccacgcag cttcatttga tggaagaaat caaaaaagtg
     9541 aagcgcgtgc tgcaaaaagc cattcccggc gcgccgcacg aaatcatcgt cgtactcgat
     9601 gccaatatcg ggcaaaacgc cgtcaaccaa gtcaaagcct ttgacgacgc attggggctg
     9661 acggggctta tcgttaccaa actcgacggc acggcaaaag gcggcatcct cgccgcgctt
     9721 gcttccgacc gccccgtccc cgtccgctac atcggcgtgg gcgaaggcat agacgacctg
     9781 cgcccgtttg acgcgcgcgc gtttgtggac gcactgctgg attgagccga aatgccgtcc
     9841 gaaaacggca gaccgccgc
//
LOCUS       DAWTQS010000069         9320 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.069, whole genome shotgun sequence.
ACCESSION   DAWTQS010000069 DAWTQS010000000
VERSION     DAWTQS010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9320
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.069"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            114..389
                     /locus_tag="WNA25_001778"
     CDS             114..389
                     /locus_tag="WNA25_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824477.1"
                     /translation="MNVVVSAGLTKLAYKKMMRRHSRYALFLSGVAACAAVAWIFKLL
                     LGSAALGGLSGEAVSEYAFAVWLVSMLTLPKRLTRAPVQPVVFHRKK"
     gene            484..1107
                     /gene="rsmG"
                     /locus_tag="WNA25_001779"
     CDS             484..1107
                     /gene="rsmG"
                     /locus_tag="WNA25_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFA9824478.1"
                     /translation="MERKERLRAGIAAMGPDISETAQDRLLAYVDLLKKWNKTYNLTA
                     LRDEEKMIVHHLLDSLTLLPYIEGAQTMLDVGSGGGQPGIPAAVCRPDVQITLLDANT
                     KKTAFLRQAAIELGLDNVRVVSGRVEAVSDVRADVVTSRAFAELADFVSWTAHLLKDG
                     GYWAAMKGVYPQGEIGRLPQDVCVEKVQRLDVPGLDAERHIVILSKR"
     gene            1207..1980
                     /locus_tag="WNA25_001780"
     CDS             1207..1980
                     /locus_tag="WNA25_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFA9824479.1"
                     /translation="MSANILAIANQKGGVGKTTTTVNLAASLASRGKRVLVVDLDPQG
                     NATTGSGIDKAGLQSGVYQVLLGDADVQSAAVRSKEGGYAVLGANRALAGAEIELVQE
                     IAREVRLKNALKAVAEDYDFILIDCPPSLTLLTLNGLVAAGGVIVPMLCEYYALEGIS
                     DLIATVRKIRQAVNPDLDITGIVRTMYDSRSRLVAEVSEQLRSHFGDLLFETVIPRNI
                     RLAEAPSHGMPVMAYDAQAKGAKAYLALADELAARVSGK"
     gene            complement(2726..3310)
                     /gene="rnhB"
                     /locus_tag="WNA25_001781"
     CDS             complement(2726..3310)
                     /gene="rnhB"
                     /locus_tag="WNA25_001781"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFA9824480.1"
                     /translation="MHILTAGVDEAGRGPLVGSVFAAAVILPETFDLPGLTDSKKLSE
                     KKRDALAEMIKEQAVAWHVAASTPEEIASLNILHATMLAMKRAVYGLAARPEKIFIDG
                     NRIPEHLGIPAEAVVKGDSKIIEISAASVLAKTARDAEMYALAQRRPQYGFDKHKGYG
                     TKQHLEALKQYGVLPEHRRDFAPVRNLLAQQALF"
     gene            complement(3378..5273)
                     /gene="mnmG"
                     /locus_tag="WNA25_001782"
     CDS             complement(3378..5273)
                     /gene="mnmG"
                     /locus_tag="WNA25_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFA9824481.1"
                     /translation="MTHMIYPKTYDVIVVGGGHAGTEAALAAARMGAQTLLLTHNIET
                     LGQMSCNPSIGGIGKGHLVRELDALGGAMALATDKSGIQFRRLNASKGAAVRATRSQA
                     DRILYKASIREMLENQENLDLFQQAVEDVTLEGERISGVITAMGAEFKARAVVLTAGT
                     FLSGKIHIGLENYEGGRAGDPAAKSLGGRLRELKLPQGRLKTGTPPRIDGRTIDFSQL
                     TEQPGDTPVPVMSVRGNAEMHPRQVSCWITHTNTQTHDIIRSGFDRSPMFTGKIEGVG
                     PRYCPSIEDKINRFADKDSHQIFLEPEGLTTHEYYPNGISTSLPFDIQIALVRSMKGL
                     ENAHILRPGYAIEYDYFDPRNLKASLETKTIEGLFFAGQINGTTGYEEAAAQGLLAGA
                     NAVQYVRGQDPLLLRREQAYLGVLVDDLITKGLNEPYRMFTSRAEYRLQLREDNADMR
                     LTEDGYKIGLVGEAQWRMFNEKREAVEREIQRLKTTWYAPQKLAEDEQIRVFGQKLSR
                     EANLHDLLRRPNLDYAALMTLEGAIPSERLSAEVVEQVEIQVKYQGYIDRQNEEIDSR
                     RDIETLKLPDGIDYGRVKGLSAEVQQKLNQHKPETVGQASRISGVTPAAVALLMVHLK
                     RGFKDAK"
     gene            complement(5446..6873)
                     /locus_tag="WNA25_001783"
     CDS             complement(5446..6873)
                     /locus_tag="WNA25_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HFA9824482.1"
                     /translation="MNENFTEWLHGWVGAINDPMWSYLVYMLLGTGLFFTVTTGFVQF
                     RLFGRSIKEMLGGRKQGDDPHGITPFQAFVTGLASRVGVGNIAGVAIAIKVGGPGAVF
                     WMWVTALIGMSSAFVESSLAQLFKVRDCDNHHFRGGPAYYITHGLGQKWLGVLFALSL
                     IFCFGFVFEAVQTNTIADTVKAAWGWEPHYVGVALVILTAPIIFGGIRRISKAAEIVV
                     PLMAVLYLFIALFIILTNIPMIPDVFGQIFSGAFKFDAAAGGLLGGLISQTMMMGIKR
                     GLYSNEAGMGSAPNAAAAAEVKHPVSQGMIQMLGVFVDTIIVCSCTAFIILIYQQPYG
                     DLSGAALTQAAIVSQVGQWGAGFLAVILFMFAFSTVIGNYAYAESNVQFIKSHWLITA
                     VFRMLVLAWVYFGAVANVPLVWDMADMAMGIMAWINLVAILLLSPLAFMLLRDYTAKL
                     KMGKDPEFKLSEHPGLKRRIKSDVW"
     gene            7032..8024
                     /gene="pdxA"
                     /locus_tag="WNA25_001784"
     CDS             7032..8024
                     /gene="pdxA"
                     /locus_tag="WNA25_001784"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFA9824483.1"
                     /translation="MKQPVFAVTSGEPAGIGPDICLDLAFARLPCRCAVLGDKHLLRA
                     RAEALGKSVVLRDFDPESGGAHGEGGLEVLHIPAAEAVEAGWLNPANAAYVLQLLDTA
                     LAGISDGIFDGIVTAPLHKGIINAARASTGFFSGHTEYLAEKSGTGQVVMMLAGKGLR
                     VALVTTHLPLKDVAAAITQPLIESVARILHHDLKHKFGIKNPKILVAGLNPHAGEGGH
                     LGHEETDTIIPALENLRREGINLAGPYPADTLFQPFMLEGADAVLAMYHDQGLPVLKY
                     HSFGQGVNITLGLPFIRTSVDHGTALDLAATGRADSGSLITAVETAVEMARGSL"
     gene            complement(8254..>9320)
                     /locus_tag="WNA25_001785"
     CDS             complement(8254..>9320)
                     /locus_tag="WNA25_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFA9824484.1"
                     /translation="AVPAAETSEKRSTANRSGSRANNRRQNPRRSKREGSKIEVREAA
                     GKTAGQKARADKAETRNNGNRRRNERGDRATERANEAEIQSRNVQPAAPVADAAPPET
                     EGQTGKRRRNGSRNERGQTAPETAAVAETAVQTAENTPPEPYTAEDKGSKPKSERNRR
                     ERDSRDAKERRERNNQRDRRQNGKKRNIPSAAKIEQYLNIHDTADKVRSAAAHVFGET
                     DANAPITVSIADPLIATPVQTASSAVSNGDALIYDAAEKIRRAAADILPEGAAPKAAA
                     QEMPSETATFTAAAEQARETAQTGGLVLIETDPAALKAWAAQPEVQAGRGLRRSEQPK
                     PSEAATVPAEEMIQVETRQG"
BASE COUNT         1800 a         2416 c         2923 g         2181 t
ORIGIN      
        1 ttgcatgtgc gttcggacgg gcggggctgc tggtgtcggt gtcggccggc ggattcggcc
       61 ggcgtggcgg ttgggacggc actgtttggt ttgtgttcgg tgtgtttgcg tttttgaatg
      121 tggttgtgtc ggcgggcctg acgaaactgg cgtacaaaaa gatgatgcgg cggcattcgc
      181 gttacgcgct gtttctgtcg ggcgtggcgg cttgcgcggc ggtggcttgg attttcaagc
      241 tgttgttggg cagtgccgcc ttgggcgggc tgagcgggga ggcggtgtcg gaatatgcgt
      301 ttgccgtgtg gctggtgtct atgctgacgc tgccgaaacg cctgacgcgc gcgccggtgc
      361 agcctgtggt gttccacagg aaaaaatagg acggaacgga aatgccgtct gaaacccgat
      421 acgcggtttc agacggcatg tttttccgct aacattacgc ctgaatatgg acaggaagca
      481 gatatggaac gtaaagaacg cctgcgtgca ggcattgccg cgatggggcc ggatatttcg
      541 gaaacggcgc aggacaggct tttggcctat gtggatttgt tgaaaaagtg gaacaaaacc
      601 tacaatctga ccgccctgcg cgacgaggaa aaaatgattg tccatcatct tttggacagc
      661 ctgacgctgc tgccctatat cgaaggcgca cagacgatgc tggatgtcgg ttcgggcggc
      721 ggccagcccg gcattccggc ggcggtgtgc cgtccggatg tgcagataac cttgttggat
      781 gcgaatacga agaaaacggc ttttttgcgg caggcggcta tcgagttggg gttggacaat
      841 gtgcgcgtgg tgtcggggcg cgtggaggcg gtttcggacg tgcgtgccga cgtggttacc
      901 agccgtgcgt ttgcggaact ggcggatttt gtgtcgtgga cggcgcacct gttgaaagac
      961 ggcggctact gggcggcgat gaagggcgta tatccgcagg gggaaatcgg ccgcctgccg
     1021 caggatgtgt gcgtcgaaaa agtccaaagg ctcgacgtgc cgggcttgga tgcggaacgc
     1081 catatcgtca tcctgagcaa gcgttgagcg cgcttcagac ggcatgaata ccttttttgt
     1141 gcggataaag gtaaaattcc gcactgtttt tctttttttc aacatcagac gggacacggg
     1201 cgggacatga gtgcgaacat ccttgccatc gccaatcaga agggcggtgt gggcaaaacg
     1261 acgacgacgg taaatttggc ggcttcgctg gcatcgcgcg gcaaacgcgt gctggtggtc
     1321 gatttggatc cgcagggcaa tgcgacgacg ggcagcggca tcgacaaggc gggtttgcag
     1381 tccggcgttt atcaggtctt attgggcgat gcggacgtgc agtcggcggc ggtacgcagc
     1441 aaagagggcg gatacgctgt gctgggtgcg aaccgcgcgc ttgccggcgc ggaaatcgag
     1501 ctggtgcagg aaatcgcccg ggaagtgcgt ttgaaaaacg cgctcaaggc agtggcggaa
     1561 gattacgact ttatcctgat cgactgtccg ccttcgctga cgctgttgac gcttaacggc
     1621 ttggtggcgg cgggcggcgt gattgtgccg atgttgtgcg aatattacgc gctggaaggg
     1681 atttccgatt tgattgcgac cgtgcgcaaa atccgtcagg cggtcaatcc cgatttggac
     1741 atcacgggca tcgtgcgcac gatgtacgac agccgcagca ggctggttgc cgaagtcagc
     1801 gaacagttgc gcagccattt cggggatttg ctttttgaaa ccgtcatccc gcgcaatatc
     1861 cgccttgcgg aagcgccgag ccacggtatg ccggtgatgg cttacgacgc gcaggcaaag
     1921 ggtgccaagg cgtatcttgc cttggcggac gagctggcgg cgagggtgtc ggggaaatag
     1981 gtcaatccaa atcgggctgc ccgtgccttt atgctgtttg gccgggtgcg ttaatatggc
     2041 ggattaaaat aaaaatactt aatatcgtca tttatagcgg attaacaaaa accggtacgg
     2101 cgttgccccg ccccggctca aagggaacga ttccctaagg cgcccaagca ccaggcgaac
     2161 cggttccgta ctatccgtac tgtctgcggc ccgccgcctt gtcctgattt ttgttaatcc
     2221 gctatatcgt cattcccgca aaaacaaaaa aatcaaaaac agaaaactga aatatcgtca
     2281 ttcccgcgca ggcgggaatc taggtctgtc ggtacggaaa cttatcggga aaaacggttt
     2341 ttccaaccct gagactccgg attcctgttt tcgcgggaat ccggtttttt gagtttcagt
     2401 catttttgat aaattcttgc agctttgagt ttctagattc ccgcttttgc gggaatggcg
     2461 gtttggaagt tacctgaaat tcaaaaaaaa aacggaaacc ggacggattg gattcccgcc
     2521 tgcgcgggaa tgacggattt taggtttttt tgattttctg tttttcgcgg gaatgacggt
     2581 tgggaagttt cccgaaactt aaaaacaact gaaacccaaa aaactaaatt accgcctgcg
     2641 ggggaatgac ggttcgggtt ctttctcttt gaagttgcga tgctggaaat gccgtctgaa
     2701 gttcagacgg catttttgtg ccggtttaaa acaaggcctg ctgcgcgagc aggtttctga
     2761 cgggggcgaa gtcgcggcgg tgttcgggca gcacgccgta ttgtttgagg gcttccagat
     2821 gctgctttgt gccgtaacct ttgtgtttgt caaaaccgta ttggggacgg cgttgcgcca
     2881 gtgcgtacat ttccgcatcg cgtgcggtct ttgccaaaac ggatgcggcg gagatttcga
     2941 tgattttgct gtcgcctttg acgactgctt cggcagggat gcctaaatgt tcgggaatgc
     3001 ggttgccgtc gatgaatatt ttttcgggac gcgcagccaa accgtaaacg gcacgtttca
     3061 tggcgagcat ggtggcgtgc aggatgttga ggcttgcgat ttcttcgggc gtggaggcgg
     3121 cgacgtgcca cgcgaccgcc tgttctttaa tcatttcggc aagcgcgtcg cgttttttct
     3181 cgctgagttt tttggagtcg gtcagtccgg gcaggtcgaa tgtttccgga aggatgacgg
     3241 cggcggcaaa cacgctgccg accaaaggtc cgcgtcctgc ctcgtccacg ccggcggtca
     3301 gtatgtgcat gatgtttcct gtcgggatgg tacgatgccg tctgaaaatg gtttcagacg
     3361 gcatcgtacc gatgtgttta tttcgcgtct ttaaacccgc gcttcaaatg caccatcagc
     3421 agtgcgacgg cggcaggcgt aacgccggaa atgcggcttg cctgcccgac ggtttcgggt
     3481 ttgtgctggt tgagcttttg ttgcacttct gccgacaagc ctttgactct gccgtaatcg
     3541 atgccgtcgg gcagttttaa ggtttcgatg tcgcggcggc tgtcgatttc ttcgttttgg
     3601 cggtcgatat agccttggta tttgacttgg atttcgactt gttcgaccac ttcggcggag
     3661 aggcgttcag acggtatcgc gccttcgagc gtcatcagcg cggcgtaatc gaggttcggg
     3721 cggcgtaaca aatcgtgcag gttggcttcg cggctgagtt tttgtccgaa cacgcggatt
     3781 tgttcgtctt cggcgagttt ttgcggcgcg taccacgttg ttttcaaacg ttggatttcg
     3841 cgttcgacgg cttcgcgttt ttcgttgaac atgcgccatt gcgcttcacc caccaagccg
     3901 attttgtagc cgtcttcggt caggcgcata tcggcgttgt cttccctgag ttgcaggcgg
     3961 tattcggcgc ggctggtaaa catccggtaa ggttcgttca ggcctttggt aatgaggtcg
     4021 tccaccaata cgccgaggta ggcttgttcg cggcgcagca ggagcggatc ttgtccgcgg
     4081 acgtattgca cggcgttcgc gcccgccagc aatccttgcg cggcggcttc ttcatagcct
     4141 gtcgtgccgt tgatttgccc ggcgaaaaac agtccctcaa tggttttggt ttcgaggctt
     4201 gctttgaggt tgcgcggatc gaagtagtcg tattcgatgg cgtagccggg gcgcaggata
     4261 tgggcgtttt caagaccctt catactgcgc acgagcgcga tttggatgtc gaacggcagg
     4321 ctggtggaga taccgttggg gtagtattcg tgcgtggtca gaccttcggg ttcgaggaaa
     4381 atctggtggc tgtctttgtc ggcgaagcgg ttgattttgt cttcgataga cggacaataa
     4441 cgcggaccca cgccttcgat tttgccggta aacatcgggc tgcggtcgaa gcctgagcgg
     4501 atgatgtcgt gggtttgcgt gttggtatgc gtaatccagc aggacacttg gcgcgggtgc
     4561 atttcggcgt tgccgcgcac ggacatgacg ggaacgggcg tgtcgccggg ctgttcggtg
     4621 agttgggaaa agtcaatcgt gcgtccgtca atacgcggcg gcgtgccggt tttcagacgg
     4681 ccttgcggca gcttcaattc gcgcaaacgt ccgccgagcg atttggcggc tgggtcgccg
     4741 gcgcggccgc cttcgtagtt ttccaaaccg atgtggattt tgccggacaa gaacgtgcct
     4801 gcggtcagca ccacggcgcg tgctttaaac tccgcgccca tcgcggtgat cacgccgctg
     4861 atgcgttcgc cctcgagtgt tacgtcttcg acggcttgtt ggaaaaggtc gaggttttct
     4921 tggttttcca acatttcgcg gatggaggct ttatacagga tgcggtctgc ctgcgagcgc
     4981 gtggctcgta ctgccgcgcc tttgctggcg ttcaggcggc ggaattggat acctgatttg
     5041 tcggttgcca acgccattgc gccgccgagc gcgtcgagtt cgcgtaccaa atgccctttg
     5101 ccgatgccgc cgatagaggg gttgcacgac atttgtccga gcgtttcgat attgtgtgtg
     5161 agcaaaagcg tctgcgcgcc catacgggcg gcggcaagcg cggcttccgt gccggcgtgt
     5221 ccgccgccga cgacgatgac atcgtaggtt ttggggtaaa tcatgtgggt catgtgtgta
     5281 ttgcctatcg gtttttcaga cggcattcca agcaggatgc cgtctgaaaa acgaaacgga
     5341 ttcaaaagta aagggttggg attgtacgcc tgttcgccct gtttttacag tgcgcggaaa
     5401 gggaaaagcc gcttcgcggg gaagcggctc cggtaagggt gggatttacc aaacatcgga
     5461 tttgatgcgg cgtttcaggc ccggatgttc ggaaagtttg aactcggggt ctttgcccat
     5521 tttcagcttg gcggtgtaat cgcgcagcag cataaacgcc aatggcgaga gcagcaggat
     5581 ggcgacgagg ttgatccacg ccatgatgcc catcgccata tccgccatat cccagaccaa
     5641 aggcacattg gcaaccgcgc cgaaatagac ccacgccaaa accagcatac ggaaaacggc
     5701 ggtaatcagc caatggcttt tgatgaattg gacgttggac tcggcatagg catagttgcc
     5761 gataacggtg gaaaaggcaa acataaacag gatgacggcg aggaaacccg cgccccattg
     5821 ccccacttgg ctgacaatcg ccgcctgcgt cagcgccgca ccgctcaaat cgccataagg
     5881 ctgttggtaa atcaagatga tgaaggcggt gcaagaacaa acgatgatgg tatcgacaaa
     5941 cacgcccagc atttgaatca taccttgcga aacagggtgt ttcacttcgg cggcggcggc
     6001 ggcgttcggc gcggaaccca tacccgcctc gttggaatac aggccgcgtt tgatgcccat
     6061 catcatcgtt tgcgaaatca gaccgccgag taagccgcct gctgccgcgt cgaatttgaa
     6121 cgcgcccgaa aaaatctgac cgaacacgtc cggaatcatc ggaatattgg tcaaaatgat
     6181 gaaaagcgcg ataaagaggt acaaaaccgc catcaggggc acgacgattt ccgccgcttt
     6241 ggatatgcgc ctgatgccgc cgaagataat cggcgcggtt aaaatcacca gggcgacgcc
     6301 gacataatga ggctcccagc cccacgccgc tttgacggta tcggcgatgg tattggtctg
     6361 aaccgcttca aacacaaaac cgaaacagaa aatcaggctc agggcgaaca acacgcccag
     6421 ccatttctgc cccagaccgt gcgtgatgta gtaggcaggg ccgccccgga aatggtggtt
     6481 gtcgcagtcg cggactttaa agagctgcgc cagcgaagat tcgacaaacg ccgaactcat
     6541 gccgattaag gcggttaccc acatccaaaa caccgcgccc ggcccgccga ctttgatggc
     6601 gattgccacg cccgcgatat tgcccacgcc cacgcggctg gcaaggccgg ttacaaatgc
     6661 ctgaaacggc gtgatgccgt gagggtcgtc cccctgtttg cggccgccga gcatttcttt
     6721 gatgctgcgc ccgaacaggc ggaattggac aaagcccgtg gttacggtga agaaaagccc
     6781 cgtacccaaa agcatataaa ccaagtatga ccacatcgga tcgttgatgg cgccgaccca
     6841 gccgtgcagc cattcggtaa agttctcgtt catatcgctt ccttaaagtt gaaacccgca
     6901 catatggcgg tatgcaagca gggtttaaat tttgtaaacg cccattctag cagattgtca
     6961 acaaaatcag aaaaatttac atcgccgcgc gcctgtggcg ttagaatcgc gttttgtttg
     7021 gagcaaacac gatgaaacag cctgtttttg ccgttacttc cggcgagcct gccggcatcg
     7081 gccccgatat ttgtttggac ttggcgtttg cacgcctgcc ctgccgctgc gcggtattgg
     7141 gcgacaaaca cctgttgcgc gcgcgcgccg aagccttggg caaaagcgtc gtcctgcgcg
     7201 acttcgatcc ggaatcaggc ggcgcgcacg gagaaggcgg gctggaagtg ctgcacatcc
     7261 ctgccgccga agcggttgag gcagggtggc tcaatcccgc caacgccgcc tatgtgctgc
     7321 aacttttgga caccgcgctc gcaggcattt cagacggcat tttcgacggc atcgttaccg
     7381 cgccgctgca caaaggcatc atcaacgccg cgcgcgcaag cacaggtttt ttcagcggac
     7441 acaccgaata tctggcggaa aaaagcggca cggggcaggt cgtgatgatg cttgccggca
     7501 aaggcctgcg cgtcgccctc gtaacgaccc acctgccgct gaaagacgtt gccgccgcca
     7561 tcacgcaacc gctgattgaa agcgtcgcac gcattttgca tcacgactta aaacacaaat
     7621 tcggcatcaa aaatcccaaa atcctagtcg ccggactcaa tccccacgcc ggcgaaggcg
     7681 gacacctcgg acacgaagaa accgacacca ttatccccgc gctcgaaaac ctgcgccgcg
     7741 aagggataaa cctcgccggc ccgtatccgg cggacacatt gttccaaccg tttatgctcg
     7801 aaggtgcgga tgccgtattg gcgatgtacc acgaccaagg gctgcccgtg ttgaaatacc
     7861 acagcttcgg gcagggcgtg aacatcacgc tggggctgcc ctttatccgc acctccgtcg
     7921 atcacggcac cgcgctcgat ttggcggcaa ccggcagggc ggactccggc agcctgataa
     7981 ctgccgtgga gaccgccgtc gagatggcgc gcggcagcct ttaaagatga taaaagaccc
     8041 gtcattcccg cgcaggcggg aatccggacc ttcgggcggc ggcaatattc aaaggttatc
     8101 cgaaagtttg aggttctaga ttcccgtttt cacgggaatg acgaaaggtg gcgggaatca
     8161 cgggaatggc gaagtttcag acggtattgc aggtatccga aaccatgcaa aaagaggttc
     8221 tgcggaacag aacctctttt tgccgccgtc ggttcagcct tgccgggttt cgacttggat
     8281 catttcttcg gcagggacgg ttgcggcttc agacggtttt ggctgttcgg aacggcgcaa
     8341 accgcgtccg gcttggactt cgggttgtgc cgcccatgcc ttcaatgcgg cagggtcggt
     8401 ttcgatcagg acgagtccgc cggtttgtgc ggtttcccgt gcctgttccg ccgcagccgt
     8461 aaaggttgcg gtttcagacg gcatttcctg tgctgcggct ttcggtgccg cgccttcggg
     8521 caggatgtcg gcggcggcac ggcggatttt ttccgccgca tcataaatca gtgcgtcgcc
     8581 gtttgaaacg gcggaagatg ccgtctgaac gggtgtggcg atgagcggat cggcaatgct
     8641 gacggtaatc ggcgcgtttg cgtcggtttc gccgaaaacg tgcgcggcgg cggaacggac
     8701 tttgtcggcg gtgtcgtgaa tattcaggta ctgctcgatt ttggcggcag acgggatatt
     8761 gcgttttttg ccgttttgac ggcggtcgcg ctgattgttg cgttcgcggc gttctttggc
     8821 atcgcggctg tcgcgttcgc ggcggttgcg ttcggatttg ggcttgctgc ctttgtcttc
     8881 tgcggtatac ggttcgggcg gcgtgttttc cgctgtctga acggctgttt cggcaacggc
     8941 ggcggtttcc ggtgcggttt ggccacgttc gttgcggctg ccgttgcggc ggcgttttcc
     9001 ggtttgacct tcggtttcgg gcggtgcggc atctgcaacg ggtgcggcag gctgtacgtt
     9061 gcggctttgg atttccgctt cgttggcgcg ttcggtggca cggtcgccgc gttcgttgcg
     9121 gcggcggttg ccgttgttgc gcgtttcggc tttgtcggcg cgcgctttct gtccggcagt
     9181 tttgcccgcc gcttcgcgga cttctatttt gctgccttcg cgtttgctgc ggcgcgggtt
     9241 ttggcggcgg ttgttggcgc ggctgccgct gcggtttgcc gtgctgcgtt tttcggaggt
     9301 ttcggcagcg ggtacggctt
//
LOCUS       DAWTQS010000070         9291 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.070, whole genome shotgun sequence.
ACCESSION   DAWTQS010000070 DAWTQS010000000
VERSION     DAWTQS010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9291
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.070"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            39..1695
                     /locus_tag="WNA25_001786"
                     /pseudo
     CDS             39..1695
                     /locus_tag="WNA25_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709079.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(2178..3590)
                     /gene="trkA"
                     /locus_tag="WNA25_001787"
     CDS             complement(2178..3590)
                     /gene="trkA"
                     /locus_tag="WNA25_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFA9824485.1"
                     /translation="MKILILGNGQVGSTVAQNLAAITNNDVTVIDIDEKALQETGSRL
                     DVQTVFGNGASPFTLERAGAEDADLLLALSRSDETNIVACKVAADLFNIPGRIARVRS
                     SEYLEYLSPKLENNENGSLSIFGITETISPEQLVTEQLAGLIDCPGALQVLRFADDRV
                     RMVIIQARRGGLLVGRSIADIAQDLPDGADCQICAVYRNNRLIVPAPQTVIIEGDEIL
                     FAAAAENIGAVIPELRPKETSTRRIMIAGGGNIGYRLAKQLEHAYNVKIIECRPRRAE
                     WIAENLDNTLVLQGSATDETLLDNEYIDEIDVFCALTNDDESNIMSALLAKNLGAKRV
                     IGIVNRSSYVDLLEGNKIDIVVSPHLITIGSILAHIRRGDIVAVHPIRRGTAEAIEVV
                     AHGDKKTSAIIGRRISGIKWPEGCHIAAVVRAGTGETIMGHHTETVIQDGDHIIFFVS
                     RRRILNELEKLIQVKMGFFG"
     gene            complement(3688..5210)
                     /locus_tag="WNA25_001788"
                     /pseudo
     CDS             complement(3688..5210)
                     /locus_tag="WNA25_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221888.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
     gene            complement(5517..6323)
                     /locus_tag="WNA25_001789"
     CDS             complement(5517..6323)
                     /locus_tag="WNA25_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HFA9824486.1"
                     /translation="MNMKKWIAAALACSALALSACGGQGKDAAAPAANPGKVYRVASN
                     AEFAPFESLDSKGNVEGFDVDLMNAMAKAGNFKIEFKHQPWDSLFPALNNGDADVVMS
                     GVTITDDRKQSMDFSDPYFEITQVVLVPKGKKVSSSEDLKKMNKVGVVTGHTGDFSVS
                     KLLGNDNPKIARFENVPLIIKELENGGLDSVVSDSAVIANYVKNNPAKGMDFVTLPDF
                     TTEHYGIAVRKGDEATVKMLNDALEKVRESGEYDKIYAKYFAKEGGQAAK"
     gene            complement(6375..6626)
                     /locus_tag="WNA25_001790"
     CDS             complement(6375..6626)
                     /locus_tag="WNA25_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824487.1"
                     /translation="MGSIEQRLEYLEEANDVLRMQNHVLSTAFKALIRALPADTAEIA
                     VESIQLAFEDALAELSYEDSPHTDLFHDVTYAFFREKER"
     gene            6728..7498
                     /locus_tag="WNA25_001791"
     CDS             6728..7498
                     /locus_tag="WNA25_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824488.1"
                     /translation="MDTNNQDKEPNWEKRLENLRQTRRMNTAPAYVPLTKPSEVGKEE
                     PIPKLDDANRDRVLKAYLKKWQEEHNSAVADCGEEVETDPMIVLQENWLNAQASLQMP
                     VSEKHIESRRRIRFDAKKDSAEFETAQIEGGENADAAESGTAADGVAENGEGGAEEAL
                     MPVQINILNPKAVNRREVFCLSEQELTERLIKRLRPHLTDTVNGMIRTAVQKQMALFT
                     YQLQQMLHEQAGAVVEDVLEHDVRKILNDIKYELKYKR"
     gene            7644..8813
                     /gene="metZ"
                     /locus_tag="WNA25_001792"
     CDS             7644..8813
                     /gene="metZ"
                     /locus_tag="WNA25_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HFA9824489.1"
                     /translation="MSKKLHPQTLAIRGGKEQTGYREHNQALFLTSSFMWDNAQHAAD
                     LFSKKIKGFTYTRTANPTIAAFEKRIAALEGAERAVATSTGMSAIQAAFFTFLQAGGH
                     VVSSRSLFGTTVGFINNIVTKFGIGVSRVSPTDINEWKAAVKANTKLLFLETPSNPLG
                     EVADLEALAELAHGIGALLVVDNSLLSPVGSQPLKHGADISVSSATKAIDGHGRVMGG
                     VLAGSEELMAQVAVYCNSCGLAMSPFNAWQLLSGVETLSLRMEKQFDNALRIAQWLQE
                     QPQVQAVYYTGLPDHPQAELIRKQQNGGGIVIGFEVADQAAAWKVVDGVELFSRTANL
                     GDVRSTITNPWTTTHGRMQPEEKLAAGIRPGLVRLSVGLEYVGDLIDDLKQALAR"
     gene            8908..9114
                     /locus_tag="WNA25_001793"
     CDS             8908..9114
                     /locus_tag="WNA25_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HFA9824490.1"
                     /translation="MDEAVFADWALKICLTGLIIFLGFIVWNLGKESKAGKFGIAVLF
                     LVLGLGVFGFVFKELLIKFLVLPK"
BASE COUNT         2127 a         2280 c         2714 g         2170 t
ORIGIN      
        1 aatgttaagc agaaacgatt gtctcaaaaa aagcaacttt ggataaatac aatcaagaat
       61 tggctgaact aggtgaaaat gaaaagaaaa aagaagaact agaaaaaaag taaaacagca
      121 agaaaacaaa attcagatca gaaaaaggca catagaacgt ctaaaagaag acatagaaaa
      181 ggttgcggta ctcgaagaag aaatcaaaag atctgaaaag ttcttcgatg aaaacggttg
      241 gcttaaagac agcattatcg acgacaaaac aaaaaacgcc gtcaacctca aaatgagcaa
      301 cggcgcgcgt tggacggtta ccaatgattc aatgctgaaa gagttggatt tgtcggaaga
      361 cgcgcaggtc gaatttagcg acaacaataa attcgtcaaa gtgtccgtga gcaagctcaa
      421 gggcgatggc ggcgtgttca aaatgtatgg cgacatcgtc aaaggagaat cggacaagct
      481 gattacccga aaaggcagcg aagggacgca catcatcgaa tatatggacg atgccaaggc
      541 aaaaacgacg ggaagggaat atctgaaact ggtcgaaaac aagggcaatc aagaagacaa
      601 taaagcatca aataaagcat catacaaatt gaatgtccgc tgtacggaac agggagggtg
      661 gtgttttgcc ttgggtgagt cgggtgcttc caaaaaggtc aatatttcca cagacggtaa
      721 gcgggacttc tacctctacc cggataccct gacgccgggc gcatcgagca gcgtcctttt
      781 cggcgaggcg ttgtatcagt tgaacgcggt ttccgatgaa acgctggtgc agcgtatggg
      841 cgaaatccac gccgacggaa cgccgcagga agacaacaac gtttggatca aacgcgtcgg
      901 cggaaaattc tccggcagcc gcagcgatta ccgcgtgggc ggttacggca accgctattg
      961 gggctttgcc ggcggcttca acaggacggg gttcggcgat aaatggattc attacaaagg
     1021 gctgatgctc cgccacctcc aatcgtccta cgcttccgaa gactacgtcg gcagcggcaa
     1081 aatcttacgg cagggcggca ggtgtttatt ccgcctggct caaccgggaa agcagggctt
     1141 attatgattt ggtcgccaac atcgcccgat ataagggcag ctacgggctg accaattacg
     1201 ccggcaaacg ggtcgaatcg gacgaggcgc gcctgaatgc ctatatgctt tccgccgaag
     1261 ccggtaggcg gatggaaaaa caagacggcg gcaaaaacct atcggtggca gccggaagtg
     1321 cagttgtctt actggtttac gcgcggctac ggctttccgc tgtcaaacgg gctgtctgcc
     1381 gagacggaca acttccgcag cctgatgggg cggttcggct ttcgggcggg cgtggacggt
     1441 ttggacggcg gcaggctgaa catctacggc aagctgatgt acaaacgcga gtttatcggc
     1501 acgatccgcc acaggttcaa cggctctgcc gtggaggaat tcaaacaccg gggcggctgg
     1561 ttggaatacg gcttgggcgt ggtgcgccgc aatgccggaa acggacggca gctttatttt
     1621 gaggcgcaaa ggtcttcgat gcacacgatg cgccagaatt ggcaggtcaa tatgggcgtg
     1681 cgcagtatgt tctgatgcgc ggatacgcct gaaaacaatc cggaacggtc agacgcgttc
     1741 cggattgttt ttttgtcggg gcggtttgct gccatatcaa aacaggcggg cgatgccgtc
     1801 tgaaacttcg tcattcccgc gaaagcggga atctagaacc tcaaactttc agataatctt
     1861 tgaatattgc tgttgctcaa tggtttggat tcccgcaacc tttcgtcatt cccgtgaaaa
     1921 cgggaatcta gaatctctaa agcttcagct aacctttgaa tattgccgct ttccaatggt
     1981 ctagattccc gcctgcacgg gaatgacgat ttggaaatta cccgaaaccc aaaaacaact
     2041 gaaaccgaac aggccggatt cccgctacgc gggaatgacg gggggcggat gccgtctgaa
     2101 gcggtgggct gaagcccact ctactattat ttttgcttcc ataccgcttg gcggcaatgt
     2161 gtccggaatg gagcggttta tccgaaaaag cccattttga cttggatgag tttctccagt
     2221 tcgttcagga tgcgccggcg cgagacgaaa aagatgatgt ggtcaccgtc ttggatgacg
     2281 gtttcggtat ggtgtcccat aatggtttcg ccggttccgg cgcggacgac ggcggcaatg
     2341 tggcagcctt cgggccattt gatgccgctg atgcgcctgc cgatgatggc ggaagttttt
     2401 ttgtcgccgt gcgcgacgac ttcgatggct tccgccgtgc cgcgccggat ggggtggacg
     2461 gcaacgatgt cgccgcgccg gatgtgggcg agtatcgagc cgatggtgat gaggtggggg
     2521 gagacgacga tgtcgatttt gttgccttcg agcaaatcga cgtagcttga gcggttgacg
     2581 atgccgatga cgcgcttcgc gccgaggttt ttcgccaaaa gggcggacat aatgttgctt
     2641 tcgtcgtcgt tggtcagggc gcagaatacg tcgatttcgt cgatgtattc gttgtcgagc
     2701 agggtttcgt cggttgccga accttgcagg acgagggtgt tgtcgaggtt ttcggctatc
     2761 cattcggcac ggcgcggccg gcattcgatg attttgacgt tgtatgcgtg ttcgagctgc
     2821 ttggcgaggc ggtagccgat gttgccgccg ccggcaatca tgatgcggcg ggtgctggtt
     2881 tctttggggc gcaattcggg tatgaccgcc ccgatgtttt cggcggcggc ggcaaacagg
     2941 atttcgtcgc cttcgatgat gacggtttgc ggcgcgggga cgatgaggcg gttgttgcgg
     3001 taaacggcgc agatttggca gtcggccccg tcgggcaaat cttgggcgat gtcggcaatg
     3061 ctgcgtccga caagcagccc gccgcgccgc gcctgtatga tgaccatccg cacgcggtcg
     3121 tctgcaaaac gtaaaacctg caatgcgccc gggcagtcta tcaggccggc aagctgttcg
     3181 gtaacgagct gttcggggct gatggtttcg gttatgccga atatggaaag gctgccgttt
     3241 tcgttgtttt cgagcttggg gctgaggtat tcgaggtatt cgctggaacg gacgcgcgcg
     3301 atgcggccgg ggatgttgaa caggtcggcg gcaactttgc aggcgacgat gttggtttcg
     3361 tcgctgcggg agagcgcgag cagcaagtcg gcatcttccg cgccggcgcg ttctaatgtg
     3421 aagggggatg cgccgttgcc gaaaacggtt tggacatcga ggcggctgcc tgtttcctgc
     3481 aatgcttttt cgtcgatgtc gataacggtt acgtcgttgt tggtgatggc ggcaaggttt
     3541 tgcgcgacgg tagaacctac ctgcccgttg cctaaaatga ggattttcac ggtatgggtc
     3601 gccgggtgat gaaatggtgg attgtaccgc aaccggcggc ggaaatgccg tctgaaggca
     3661 gattttgcgt tcagacggca ttgcgcttca agactcgacg gggatgatgc cgattttcgc
     3721 ctgccatttg cgcggggcgg tggcgtggat ggattcgcct ttgctgtcca cggcgacggt
     3781 tacgggcata tctttgactt caaattcgta aacggcttcc atacccaatt cggggaacgc
     3841 caagactttg gaagatttga tggcttttgc cacgaggtat gccgcgccgc cgactgccat
     3901 gaggtacacg gccttgttgt cggcgatggc ttcgcaggtg gccgcgccgc gctcggattt
     3961 gccgatcatg cccaagaggc cggtttgttt gagcatttgg cgggtaaatt tgtccatgcg
     4021 ggtggctgtg gtcggacctg cgggaccgac gacttcatcg ccgaccggat cgacggggcc
     4081 gacgtagtaa atcaggcggt tggtgaaatc gacgggcaac tcctcgcctt tgtcgagcat
     4141 attgacgagg cgtttgtgcg cggcatcgcg gccggtgagg attttgccgt tcaacagcaa
     4201 tacgtcgccg gttttccagc ttgccacttc ttctttggtc agcttatcga catcgacgcg
     4261 tttgccgttg tcggggctgt aagtcagatc gggccagtct tcgacgcgcg gcggcgtgag
     4321 ttcgacagga cctgagccgt ccaattcaaa ttcgacgtgg cgggtggcgg cacagttggg
     4381 aatcatggca atcggtttgg aggcggcatg ggtcgggtaa tcgaggattt tcacgtccaa
     4441 cacggtggtc agaccgccca agccttgcgc gccgatgccc agcgcgttga ccttttcaaa
     4501 gagttcgagg cgcagggctt cggtggtgga caattccgcg ccggacgcgg ctttttcctg
     4561 caattcttgg atgtcgatgt ggctcatcag ggattctttc gccatcaaca cggctttttc
     4621 gggcgtgccg ccgatgccga tgcccaagat gccgggagga caccagcccg cgcccatcgt
     4681 cgggatggtt ttcaataccc aatcgacgat gttgtcggaa gggttgagca taggagtttg
     4741 gatttgtttt cagagccgcc gccttttgcc gcgcaggtta cttcgacttt accgcccggc
     4801 acgatgctca tgtggatgac ggcgggggtg ttgtctttgg tgttttggcg tttgccggcc
     4861 ggatcggcga ggacggaagc gcgcagggtg ttgccttccc aagtgtaggc gcggcgtacg
     4921 ccttcgttaa ccatctcttc cacgctcatg tccgcatccc attgcacatc cataccgact
     4981 ttgaggaaga cggttgcgat acctgtgtct tggcagatgg ggcggttgtt ttcggcacac
     5041 atacggctgt tgaccaaaat ctgcgtcatc gcgtctttgg cggcgggatt ttcttccttc
     5101 tgccacgcct tataaagcgc gtcgatgtag tcttttggat ggtagtagct gatgaattgg
     5161 aaggcatcgc agatactttg aataaagtct tcttgcttga taacggtcat gattgtgttc
     5221 cttttcaagg ttgtggcggt aagtgtctgc tgctgtttta aacaaccttt ttgaaggctg
     5281 ttttttatca ggttgaaata gtgccagcgt ttgtacggtg aagacaatac ataattctaa
     5341 tctcttgatt atcaatttgt tttaataaaa tactgtttta ttcggatttt gatatctgta
     5401 atctttgcct gttgctttgc ggtagggaga aatcagcagg cagttcattg tggaccggtt
     5461 gatgaacaat gccgtctgaa agggcttcag acggcatttt gttcggacgg gcgggcttat
     5521 ttcgcagcct gtccgccctc tttggcaaaa tatttggcgt agatcttgtc gtattcgccg
     5581 ctttcgcgta ctttttccaa cgcatcgttc agcattttga cggttgcttc gtcgcctttg
     5641 cgtaccgcga tgccgtagtg ttcggtggtg aagtcgggca gggtaacgaa gtccattcct
     5701 ttggccgggt tgtttttcac ataattggcg atgaccgcgc tgtcgctgac cacggaatcc
     5761 aagccgccgt tttccagttc tttgataatc agggggacgt tttcgaagcg cgcgattttc
     5821 ggattgtcgt tgcccaagag tttggaaacg gagaaatcgc ccgtgtggcc ggtaaccacg
     5881 ccgactttgt tcatcttttt caaatcttcg gaagaagata cttttttgcc tttcggaacg
     5941 aggacgactt gggtgatttc aaaatacggg tcgctgaaat ccatagactg tttgcggtcg
     6001 tcggtaatgg ttacgcccga catcacaacg tccgcatcgc cgttgttcaa ggcggggaaa
     6061 aggctgtccc acggctggtg tttgaattcg attttaaaat tgcccgcctt cgccatcgcg
     6121 ttcatcaaat ccacgtcgaa accttcgaca ttgcctttcg agtctaaaga ttcaaagggg
     6181 gcaaactcgg cgttggaagc cacgcggtac actttgccgg ggttggcggc aggcgcggcg
     6241 gcatctttgc cctgaccgcc gcaggcagac agcgcgagtg cggaacaggc aagggcggcg
     6301 gcaatccatt ttttcatatt catcaaaatt cctttcggta atcaaaaagc ctaaaaaatc
     6361 agtttaacat aaaattaacg ttccttttca cggaaaaacg cataagtaac gtcgtggaac
     6421 aaatccgtat gcgggctgtc ctcatagctc aattctgcca aggcgtcctc aaaagcaagc
     6481 tgaatcgact cgaccgcgat ttcggcggtg tctgcgggaa gggcgcggat cagggctttg
     6541 aaggcggtgg acaggacgtg gttctgcata cgcagcacat cgtttgcttc ttccagatat
     6601 tccaaacgct gttcgatgct gcccatgatg tatcctcgcg aatctgttaa aatgcgccat
     6661 tatatcaaac tgcgctttgg gcgcgggttt gcccgcctgc aaagattaag aaccggaagc
     6721 cgaaaaaatg gatacaaaca atcaggataa agagccgaat tgggaaaaac gcctggaaaa
     6781 cctgcgtcag acgcgccgta tgaataccgc gcctgcctat gtgccgctga ccaaaccttc
     6841 tgaagtcgga aaggaagagc ccataccgaa attggacgat gcaaaccgag accgtgtgtt
     6901 aaaggcgtat ctgaagaaat ggcaggaaga acacaacagc gccgttgccg attgcggcga
     6961 ggaagtcgaa accgacccga tgatcgtcct tcaggaaaac tggctcaacg cgcaggcttc
     7021 cctgcaaatg ccggtttcgg aaaaacacat cgaatcgcgg cggagaatcc ggttcgatgc
     7081 caaaaaagac agtgccgaat ttgaaaccgc acagattgag ggcggggaaa acgccgatgc
     7141 ggcagaatcc ggtacggcgg cagacggggt tgcggaaaac ggcgaaggcg gtgcggaaga
     7201 ggcgttgatg cccgtccaaa tcaatatcct gaacccgaaa gccgtcaacc ggcgcgaagt
     7261 gttctgcctg tccgaacagg agctgaccga acggttgatc aaacgcctgc gcccgcatct
     7321 gaccgatacc gtcaacggca tgatccgtac ggcggtgcag aagcagatgg cgctctttac
     7381 ttatcagctc cagcaaatgc tgcacgagca ggcgggtgcg gtagtcgaag acgtgttgga
     7441 acacgatgtc cgtaaaatcc tcaacgacat caagtacgaa ttaaaataca aacgctgatt
     7501 gccatgccgt ctgaaagccg tgttcagacg gcattttcct gcccgtccga cttgaaagcc
     7561 tcatggaaca ggcgcaaaat attaataaat taaacaacgg gtgcacgccc cgttgccgaa
     7621 cggacaaaac cgaggaatca ccgatgagca aaaaacttca tccgcaaacg ctcgccatac
     7681 gcggcggcaa agaacaaacc ggataccgcg aacacaatca ggcattgttc ctgaccagca
     7741 gcttcatgtg ggacaacgcc caacacgctg ccgatttgtt ttcaaaaaaa atcaaaggct
     7801 tcacttatac ccgtaccgcc aacccgacca tcgcggcctt tgaaaaacgc atagccgctt
     7861 tggaaggcgc ggaacgcgcg gtcgccactt cgaccggcat gtccgcgatt caggcggcgt
     7921 ttttcacctt cctgcaggcg ggcggccatg tggtttccag ccgcagcctg ttcggcacga
     7981 ccgtcggctt tatcaataac atcgttacca aattcggcat cggagtgagc cgcgtgtcgc
     8041 cgactgatat aaatgaatgg aaagccgccg tcaaagccaa caccaaactg ctgtttttgg
     8101 aaacgccgtc caaccccttg ggcgaagtgg ccgacttgga agccttggcg gaattggcgc
     8161 acggcatcgg cgcgctcttg gtggtggaca acagcctgct gtcgcccgtc ggctcgcagc
     8221 ctttgaaaca cggcgcggat atttccgttt cctccgccac caaagccata gacggacacg
     8281 ggcgcgtgat gggcggcgtg ttggcgggtt cggaagaact gatggcgcag gtcgccgttt
     8341 actgcaactc ttgcggtttg gcgatgtcgc cgtttaacgc gtggcagttg ttgagcggcg
     8401 tggaaaccct gtcgctgcgt atggaaaaac agtttgacaa cgccttgaga atcgcgcaat
     8461 ggctgcaaga gcagccgcag gttcaagcag tgtactacac cggattgccc gaccatccgc
     8521 aggcggagct tatccgcaaa cagcaaaacg gcggcggcat cgtcatcggt tttgaagtcg
     8581 ccgaccaagc agccgcgtgg aaagtcgtgg acggcgtgga actcttttcc cgtaccgcca
     8641 acctcggcga cgtgcgctcg accatcacca atccgtggac aaccacgcac ggcaggatgc
     8701 agcccgaaga aaaactcgcc gccggtatcc gccccggact ggtgcgcctg tcggtcggtt
     8761 tggaatatgt tggcgatttg attgacgatt tgaaacaggc actcgcgcgc tgattgcgtg
     8821 tttcagacgg catcggcgga aaaatgcggc agatgccgtc tgaaaccctt attgcgtatg
     8881 gcatacacat caacaatagg aaaaaatatg gatgaagcgg tttttgccga ttgggcgttg
     8941 aaaatctgcc tgaccggttt gattattttt ttgggtttta tcgtttggaa tttgggcaag
     9001 gagtccaaag cgggcaaatt cggcatcgcc gttttgtttt tggttttggg cttgggtgtg
     9061 ttcggttttg tgttcaaaga gttattgatt aagtttttgg tattgcccaa ataggtttgc
     9121 cgtctgtcga aatgccgtct gaaatcataa aaagtaatct gcaccctaaa agtttgattt
     9181 aaccgccaac tgattaaggt gcgggttttt atgacaaaat aaaaatcatc tttccgcaat
     9241 cattcccacg gatgcgaaag cctgtctttt tgaatcccgg ataattttac a
//
LOCUS       DAWTQS010000071         9223 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.071, whole genome shotgun sequence.
ACCESSION   DAWTQS010000071 DAWTQS010000000
VERSION     DAWTQS010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9223
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.071"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(102..2954)
                     /gene="gcvP"
                     /locus_tag="WNA25_001794"
     CDS             complement(102..2954)
                     /gene="gcvP"
                     /locus_tag="WNA25_001794"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFA9824491.1"
                     /translation="MKLSELFNPNEFAARHLSFGDEAALLAAVGEKSMDDFVGNTLPQ
                     SIRMPSELDLPEALTEADALAKLKGIASKNVINKSYIGLGYYPTRVPNVILRNVLENP
                     GWYTAYTPYQAEIAQGRLEALLNFQQVCIDLTGFPVAGASLLDEATAAAEAMAMAHRV
                     GKVKSERFFVDARVYPQTLDVMKTRAKYFGFELVVSDFAQADEGEYFGALFQYVGKDG
                     DVQDLQDVIGRLKAKGTIVAVAADIMSLVLLKSPAELGADIALGNTQRFGVPMGFGGP
                     HAAYFAFKDEFKRSAPGRIIGVSKDASGKPALRMALSTREQHIRREKATSNICTAQAL
                     LANLAGMYAVYHGPKGVKRIANRIHTLASVFADALVSDGLKVVHEVFFDTVTVDFGSK
                     EKADQVFAAALESGYNLRRVNNTQVAAAFHETSVYEDLADLYRAFTGKDTFTFADDVK
                     GRLNAELLRQDDILQHPVYNSYHTEHEMLRYLKKLEDRDLAMNRSMISLGSCTMKLNA
                     TAEMLPITWTEFSDIHPYAPEAQTAGYRELLADMENSLKAITGFDAISFQPNSGAQGE
                     YSGMLAIRRYQEAQGEAHRNICLIPKSAHGTNPATAAMLGLKVVVVDTDEHGNVNIDD
                     LKAKAEQHRDALSAIMITYPSTHGVYEEGIRDICRIIHENGGQVYMDGANLNAQIGIM
                     QPAEVGADVLHMNLHKTFCIPHGGGGPGMGPIGLKAHLAPFAPGHTLTDTHSASAGQT
                     SVAAAAFGSASILPITWMYLTMMGKQGMEQATRWALLNANYVAKRLSEDYPILYTGKN
                     GRIAHECIVDLRPLKAESGITETDIAKRLMDYGFHAPTVSFPVAGTLMIEPTESESKA
                     ELDRFIAALKSIRREVQKVIDGEWPKDDNPLVNAPHTAADITGEWAHPYSREEAVFPL
                     PFVREHKFWPFVNRVDDVYGDRNLVCSCPPMENYED"
     gene            complement(2985..3344)
                     /locus_tag="WNA25_001795"
     CDS             complement(2985..3344)
                     /locus_tag="WNA25_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824492.1"
                     /translation="MTDRPNLLRIKPGQAEIMEYCEPEEASDPYATYRRANLMAGLPL
                     FVVILVLLNVVFPLPAHPLAWLVPAGFMVLGGGFPLSLPLVALLVPTCCILARCPPLS
                     RLLCHPCPNHPMSKNSA"
     gene            3292..3522
                     /locus_tag="WNA25_001796"
     CDS             3292..3522
                     /locus_tag="WNA25_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HFA9824493.1"
                     /translation="MISACPGFIRSRFGRSVMPSEPAGRLSQKTPYRCNFMRLYGVGF
                     ALQTAYASRSSLVSQSVHDYINNLGADKPLFI"
     gene            complement(3515..4156)
                     /locus_tag="WNA25_001797"
     CDS             complement(3515..4156)
                     /locus_tag="WNA25_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HFA9824494.1"
                     /translation="MPSEKPMNRRTFLLGAGALLLTACGRKSARTHAKIPEGSTVLAL
                     GDSLTFGYGANPGESYPAQLQKLTGWNIVNGGVSGDTSAQALSRLPALLARKPKLVIV
                     GIGGNDFLRKVPEEQTRANIAKIIETVQKENIPAVLVGVPHITLGALFGHLSDHPLYE
                     DLSEEYGIPLFGGAWAEILGNNNLKSDQIHANGKGYRKFAENLNQFLRKHGFR"
     gene            complement(4146..4697)
                     /locus_tag="WNA25_001798"
     CDS             complement(4146..4697)
                     /locus_tag="WNA25_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFA9824495.1"
                     /translation="MNYCEFAASLPENTDNPNKHYHDTQYGFPIKDDNGLFERLVLEI
                     NQAGLSWTLMLKKRQAFQTAFEGFDIDTVAAFGEADIERLLTDAGIVRNRLKIDAAIF
                     NARQIQALQQEHGSFKNWLDAHHPRSKDEWVKLFKKHFKFVGGEIVGEFLMSTGYLKG
                     AHAESCPVYRKTLKYHPKWLDAV"
     gene            complement(4701..5219)
                     /locus_tag="WNA25_001799"
     CDS             complement(4701..5219)
                     /locus_tag="WNA25_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFA9824496.1"
                     /translation="MRLFPIAAALTLAACGTVQSTQYFVLPDSRYIRPATQGGETAVE
                     VRLAEPLKRGGLVYQTDPYRINTAQNHVWADTLDDMLEAALSNAFNRLDSTRTFVPAS
                     RSGSTDKWTVYIDAFQGSYTGKTLISGYAVLPDGKNRPFHIETEQQGDGYAAMTAALE
                     QGLKQAAQQMVE"
     gene            complement(5219..6880)
                     /gene="pqiB"
                     /locus_tag="WNA25_001800"
     CDS             complement(5219..6880)
                     /gene="pqiB"
                     /locus_tag="WNA25_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFA9824497.1"
                     /translation="MTDNSPPPNGHAQARVRKNNTFLSAVWLVPLIALIAGGWLWVKE
                     IRNRGPVVTLLMDSAEGIEVNNTVIKVLSIDVGRVTRIKLRDDQKGVEVTAQLNADVS
                     GLIRSDTQFWVVKPRIDQSGVTGLGTLLSGSYIAFTPGKSGEAKDVFQVQDIPPVTAI
                     GQSGLRLNLIGKNDRILNVNSPVLYENFMVGQIESAHFDPSDQSVHYTIFIQSPNDKL
                     IHSASRFWLESGINIETTGSGIKLNSAPLPALLSGAISFDSPKTKNSKNVKSEDSFTL
                     YDSRSEIANLPDDRSLYYTAFFKQSVRGLTVGSPVEYKGLNVGMVSDVPYFDRNDSLH
                     LFENGWIPVRIRIEPSRLEINADEQSKEHWKQQFQTALNKGLTATISSNNLLTGGKMI
                     ELNDQPSASPKLRPHTVYAGDTVIATRGGGLDDLQAKLADLLDKFNNLPLDKTVAELN
                     GSLAELKSALKSANAALSSIDKLVGNPQTQNIPNELNQTLKELRITLQGVSPQSPIYG
                     DVQNTLQSLDKTLKDVQPVINTLKEKPNALIFNNSSKDPIPKGSR"
     gene            complement(6873..8333)
                     /locus_tag="WNA25_001801"
     CDS             complement(6873..8333)
                     /locus_tag="WNA25_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HFA9824498.1"
                     /translation="MGLSAGGAAAQKIFLPDTKGLFILPNLPVLHPDWVYRFFSFIMP
                     PFIFATFPMKPFAENIPHSLRGNCRDEALPPHTVDCPECGCRTDVPQLDKGEAAFCPR
                     CGHKLFRVGSHPFSGPPAYAAASLILMAFAYSMTYIEVGIPGAASVLSLPEMMRLMVF
                     QDYGFLAEVMFVLTFGAPVLFLLLCLYVYAALIRKQAYPALRLATRVMVRLRQAMMVD
                     VFFVSTLVAYIKLSSVAKVRFGPAFYLMFALSVMLIRTSVSVPQHWVYFQIGRLTGNN
                     AVQTASEGKTCCSRCLYFRDSAESPCGVCGAELYRRRPKSLSISSAFLTAAVVLYFPA
                     NILPIMISSNPAATEANTIFSGIAYMWDEGDRLIAAVIFSASILVPVLKIAAMSVLIA
                     AARFALPAGAKKLSHLYRITEAVGRWSMIDIFVIIILMCSFHTYAARVIPGSAAVYFC
                     LVVILTMLSAYYFDPRLLWDKRASDGIAFNETEKYD"
     gene            8518..9147
                     /locus_tag="WNA25_001802"
     CDS             8518..9147
                     /locus_tag="WNA25_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HFA9824499.1"
                     /translation="MSETENQALTFAKRLKADTTAVHDSVDNLVMSVQPFVSKENYIK
                     FLKLQSVFHKAVDHIYKDPELNKAISELEYMARYDAVTQDLKDLGEEPYKFDKELPHE
                     TGNKAVGWLYCAEGSNLGAAFLFKHAQKLDYTGEHGARHLAPHPDGRGKHWRAFVEHL
                     NALNLTPEAEAEAIQGAQEAFAFYKVILRETFGLPEGTEAPEGMMPHRH"
BASE COUNT         2049 a         2385 c         2636 g         2153 t
ORIGIN      
        1 agttatagtc gagatacgtg tatcggtgca accggtctgc cgttgacgaa aatgccgtct
       61 gaaagtgttt cagacggcat ttgtcttcgg ggtttcaaca gtcagtcttc ataattttcc
      121 atcggcgggc agctgcacac gaggttgcgg tcgccgtaca cgtcgtccac gcggttgacg
      181 aagggccaga acttgtgttc gcgcacgaag ggcagcggga agacggcttc ttcgcgagag
      241 tacggatgcg cccattcgcc ggttatatct gcggcggtgt gcggcgcgtt gaccagtggg
      301 ttgtcgtctt tcggccattc gccgtcgatg actttctgca cttcgcggcg aatggatttc
      361 agggcggcga tgaagcggtc gagttcggct ttgctttcgc tctcggtcgg ttcgatcatc
      421 agcgtgccgg caacagggaa ggaaaccgtc ggcgcgtgga agccgtagtc catcaggcgt
      481 ttggcgatgt cggtttcggt gatgccgctt tcggctttga gcggacgcaa atcgacgatg
      541 cattcgtgcg cgatgcggcc gtttttgcct gtatacagaa tcggatagtc ttcgctcaag
      601 cgtttagcga catagttggc gttgagcaat gcccagcgtg ttgcctgttc catgccttgt
      661 ttgcccatca tggtcaggta catccaagta atcggcagga tggatgcaga accgaaggct
      721 gcggcggcaa cggaggtttg cccggcactt gcgctgtggg tgtcggtcag ggtatgcccc
      781 ggtgcgaacg gggcgaggtg ggctttcaag ccaatcggac ccatgcccgg gccgccgccg
      841 ccgtgaggga tacagaaggt tttgtgcagg ttcatgtgca acacgtccgc gccgacttcg
      901 gcgggctgca tgatgccgat ttgggcattg aggtttgcgc cgtccatgta aacctgtccg
      961 ccgttttcgt gaatgatccg gcagatgtcg cggatgcctt cttcgtacac gccgtgtgtg
     1021 gacggatagg taatcatgat ggcggacaag gcatcgcggt gttgttcggc tttggctttc
     1081 aaatcgtcaa tattgacgtt gccgtgttcg tcggtatcga cgacgacgac tttcaaaccg
     1141 agcatggcgg cggtggcggg gttggtgccg tgggcggatt tgggaatcag gcagatgttg
     1201 cggtgtgctt cgccttgggc ttcctgataa cggcggatgg cgagcatacc gctgtattcg
     1261 ccttgtgcgc cggagttggg ctggaaggaa atcgcgtcaa agccggtgat ggctttcagg
     1321 ctgttttcca tatcagcaag caattcgcgg tagccggcgg tttgcgcttc gggagcgtaa
     1381 gggtggatgt cggagaactc agtccaagta atcggcaaca tttccgcagt cgcattgagc
     1441 ttcatggtgc agctcccgag cgaaatcatg ctgcggttca tagccaagtc gcggtcttcg
     1501 agttttttca ggtagcgcaa catttcgtgt tcggtgtggt aactgttgta cacaggatgt
     1561 tgcagaatgt cgtcttgacg cagtaattcg gcgttcagac ggcctttgac atcatcggca
     1621 aatgtgaacg tatccttgcc ggtaaacgcg cggtacaaat cggccaaatc ttcgtatacc
     1681 gacgtttcat ggaatgcagc cgcaacttga gtattgttga cgcggcgcag gttgtaaccc
     1741 gactccaaag cagcggcaaa cacttggtct gctttctctt tgctgccgaa atcgacggta
     1801 acggtatcga agaagacttc gtgaaccact ttgaggccgt ctgaaaccag cgcgtcggca
     1861 aagacggaag ccagtgtgtg aatgcggttg gcgatgcgtt tcacgccttt ggggccgtga
     1921 taaacggcgt acatgcccgc caagttcgcc agcaatgcct gtgcggtaca aatattggat
     1981 gtcgcttttt cgcggcggat gtgttgttcg cgggtggaca aagccatgcg caaggcaggt
     2041 ttgcccgatg cgtctttgga tacgccgatg atgcggcccg gggcggagcg tttgaactcg
     2101 tctttaaacg cgaaataagc ggcgtgcgga ccaccaaagc ccatcggtac gccgaaacgt
     2161 tgggtgttgc ccaaagcaat atctgcaccc aattcagccg gagacttcag caaaaccaag
     2221 ctcatgatgt cggcggcaac agcaacgatt gtgcctttgg ctttcagacg gccgataacg
     2281 tcctgcaagt cttgcacgtc gccgtcttta ccgacgtatt ggaacagcgc gccgaagtat
     2341 tcgccttcat ccgcttgggc aaaatcgctg accaccagct cgaagccgaa atatttggcg
     2401 cgggttttca tcacgtccaa agtttgcgga tacacgcgcg catccacaaa gaaacgctcg
     2461 gatttcactt tgcccacgcg gtgcgccatc gccatcgctt cggcggcggc ggtcgcttcg
     2521 tccaacaaag acgcgcccgc cacagggaaa ccggtcaaat cgatgcacac ctgctggaag
     2581 ttcaacaacg cttccaaacg accttgggca atctccgcct gatacggcgt gtaggcggtg
     2641 taccagcccg ggttttccaa tacgttgcgc aaaatcacgt tcggcacgcg ggtcgggtaa
     2701 taacccaagc cgatatagga cttgttgatc acgtttttcg acgcgatgcc tttcaatttc
     2761 gccaatgcgt ccgcctcggt cagggcttcg ggcaaatcga gttcggacgg catacggatg
     2821 ctttgcggca aggtgttgcc gacaaagtcg tccatgctct tctcgccgac agcggcaagc
     2881 aacgccgctt cgtcgccgaa actcaaatgg cgcgcggcaa attcgttggg gttgaacagt
     2941 tcggacagtt tcatttctac tccttcgggt ttgtagggct gccatcaggc agaattttta
     3001 gacatcggat gattcgggca agggtggcac aaaaggcggg ataatggcgg acaacgcgcc
     3061 agaatgcagc aggtcgggac aagcagcgcc acaagcggca gcgataaggg aaagccgccg
     3121 cccaaaacca tgaaacctgc aggcaccagc caagccaagg gatgagccgg aagcggaaaa
     3181 acaacattga gcagaaccaa aatcacgaca aacagcggca gccccgccat caggttggca
     3241 cgcctgtatg ttgcgtacgg gtcggatgct tcttcaggtt cgcaatattc catgatttca
     3301 gcctgtccgg gctttatccg cagcaggttc ggacggtcgg tcatgccgtc tgaaccggca
     3361 ggccgtcttt ctcaaaaaac gccataccgc tgcaacttca tgcggctgta tggcgtaggt
     3421 tttgcacttc agacggcata tgcgtcccga agcagtttgg tgtcccagtc ggttcatgat
     3481 tatataaaca acttgggtgc ggataaacct ttgtttatct aaacccatgt tttctcaaaa
     3541 attgattcaa attttcggcg aatttccgat agcctttgcc gttggcgtgg atttggtcgg
     3601 atttcagatt attattgccc aaaatttccg cccacgcgcc gccgaacaac ggaatgccgt
     3661 attcctcgga caaatcctca tacagcggat ggtcgctcaa atgcccgaac aacgcgccca
     3721 gtgtgatgtg cggcacgccg acgaggacgg cgggaatgtt ttccttttgc acggtttcga
     3781 tgattttcgc gatattggcg cgggtctgct cctcgggaac tttgcgcaga aagtcgttgc
     3841 cgcctatgcc gacaatcaca agcttgggtt tgcgtgccaa cagcgcgggc aggcgcgata
     3901 gggcttgcgc ggacgtatcg cccgatacgc cgccgttgac aatattccaa cccgtcagtt
     3961 tttgcagttg cgcggggtag gattcgccgg ggtttgctcc gtagccgaag gtgagcgaat
     4021 cgcccaaggc aagcacggtg cttccttcgg gaattttggc gtgggttcgg gcggattttc
     4081 tgccgcaggc ggtaagaagc aacgcgcctg cgccgaggag gaaggttctt ctgttcattg
     4141 gtttttcaga cggcatcgag ccatttcggg tggtatttca gggttttacg gtaaaccgga
     4201 cagctttcgg cgtgcgcgcc tttgaggtag ccggtactca tcagaaattc gccgacgatt
     4261 tcgccgccga cgaatttgaa atgtttttta aagagtttga cccattcgtc tttgcttcgc
     4321 ggatggtgcg cgtcgagcca gttcttgaac gaaccgtgtt cttgttgcaa cgcttggatt
     4381 tgccgtgcat tgaaaatggc ggcatcgatt ttcagacggt tgcggacaat acccgcatcg
     4441 gtaagcagcc gttcaatatc ggcttcgcca aatgcggcga ccgtatcgat gtcgaaacct
     4501 tcaaatgccg tctgaaacgc ctgccgcttc ttcagcatca gcgtccaact taatcctgcc
     4561 tgattgattt ccaacaccag ccgttcaaac agtccgttgt cgtccttaat cggaaaaccg
     4621 tattgcgtat cgtggtaatg tttgttcggg ttgtcggtgt tttcgggaag tgaggcggca
     4681 aattcgcaat agttcacggt ttactcgacc atctgttgcg ccgcctgttt cagtccctgt
     4741 tcgagtgcgg cggtcatggc ggcgtagccg tcaccctgct gttcggtttc gatatggaag
     4801 ggtctgttct taccgtcggg taggacggcg tagccgctga tgagggtttt gcccgtgtag
     4861 ctgccttgga atgcgtcgat atagaccgtc catttgtcgg tactgccgct gcgtgaggca
     4921 ggaacaaagg tgcgtgtgct gtccaaacgg ttgaatgcat tgctcaacgc cgcttcgagc
     4981 atatcgtcca aggtgtctgc ccaaacatgg ttttgtgcgg tgttgatgcg gtaggggtcg
     5041 gtttgataga ccagtccgcc gcgtttgagc ggttcggcaa gacggacttc gacggcggtt
     5101 tcgccgcctt gcgttgcagg acggatgtag cggctgtcgg gcaacacgaa atattgtgtg
     5161 ctttgcacag taccgcaggc ggcaagcgtc agggcggcgg cgatggggaa aaggcgcatt
     5221 atcggcttcc tttcgggata gggtctttgc tgctgttgtt gaaaatcagt gcgttgggtt
     5281 tttctttcaa agtgttaatg acgggttgaa cgtcttttaa ggttttgtcc aaactttgca
     5341 gcgtattttg tacgtctccg tagataggcg attgaggcga tacgccttgc agggttatgc
     5401 gcaactcttt cagagtttgg ttcagttcgt tcgggatgtt ttgcgtctgc ggattgccga
     5461 ccagtttgtc aatggagctt agggcggcat tggcggattt gagtgcggac ttgagttcgg
     5521 cgagcgagcc gttcaattcg gcaacggttt tatccaatgg cagattgttg aatttgtcca
     5581 gcaaatccgc caatttggcc tgcaagtcgt ccaaaccgcc gccccgtgtg gcgatgacgg
     5641 tatcgcctgc ataaacggta tgcggtcgca gcttgggcga ggcggaaggc tgatcgttca
     5701 actcaatcat tttgccgccg gtcagcaggt tgttgctgga gatggtggcg gtcaggcctt
     5761 tgtttaaggc cgtctggaat tgttgtttcc aatgctcttt gctttgctcg tcggcattga
     5821 tttccaaacg ggaaggctcg atgcggatgc gtacgggaat ccagccgttt tcaaacaggt
     5881 gcaggctgtc attgcggtca aaataaggga catcggaaac catgccgaca ttcagccctt
     5941 tgtattcgac aggcgaaccg acggtcagtc cgcgcacgga ttgtttgaaa aacgcggtgt
     6001 agtacagcga gcggtcgtca ggcaggttgg cgatttcgct gcggctgtcg taaagcgtga
     6061 agctgtcctc gcttttgacg tttttactgt ttttggtttt cggcgaatca aatgaaatcg
     6121 cgcctgacag cagggcaggc agaggggcgg aattgagttt gatgccgctg cctgtggttt
     6181 cgatattgat gccgctttcc agccaaaaac ggctggcgga atgaatcagt ttgtcgttgg
     6241 ggctttggat gaagatggtg taatgcacgc tttggtcgga cgggtcgaaa tgcgcgcttt
     6301 cgatttgccc gaccataaag ttttcataca aaacagggct gttgacgttg aggatgcggt
     6361 cgtttttacc aatcaaattc aagcgcagcc cgctttgccc gatggcggta acgggcggaa
     6421 tgtcctgcac ttggaacacg tcttttgcct cgccgctttt gccgggtgta aaagcgatgt
     6481 acgaacccga aagcagcgta cccaaaccgg ttacgccgct ttggtcgata cgcggcttga
     6541 ccacccagaa ctgggtatcg ctgcggatga ggccggatac gtccgcattg agttgggcag
     6601 taacttccac gcctttttgg tcgtcgcgca gtttgattcg ggtaacgcgt ccgacatcga
     6661 tgctcaatac cttaatgacc gtattgttga cttcgatgcc ttccgcgctg tccatcaaga
     6721 gcgtaaccac aggccccctg ttgcggattt ccttaaccca aagccagccg ccggcaatca
     6781 gcgcgatcag cgggaccagc cagacggcgg agaggaaggt gttgtttttg cggacgcgtg
     6841 cttgagcgtg tccgtttgga ggagggctgt tgtcagtcat atttttccgt ttcgttgaaa
     6901 gcaatgccgt ctgaagcgcg tttgtcccaa agcaggcgcg ggtcgaaata ataggcggac
     6961 agcatcgtca gaatcacgac caggcagaaa tagactgccg cactgcccgg aatgacgcgc
     7021 gcggcataag tgtggaacga acacatcaaa ataataatca caaaaatatc aatcatcgac
     7081 cagcggccga ccgcttcggt gatgcggtag aggtgcgaca atttctttgc gcccgccggc
     7141 aaagcgaacc gtgccgccgc aatcaaaacc gacattgccg caatcttcag caccggcacc
     7201 aaaatactcg cgctgaaaat aaccgccgca atcagcctgt cgccctcgtc ccacatataa
     7261 gcgatgccgc taaagatggt gttggcctcc gtggcggcag gattggacga aatcataatc
     7321 ggcaggatat tggcagggaa atacaaaaca accgccgccg tcagaaacgc cgacgaaata
     7381 ctcagacttt tcggccgtcg gcggtacagt tccgcgccgc acaccccgca gggggattcg
     7441 gcactgtcgc ggaaatacag gcagcggctg caacaggttt tgccttccga tgccgtctga
     7501 accgcattat tccccgtcag ccgcccgatt tggaaataca cccaatgctg gggaaccgat
     7561 accgaagtcc gaatcagcat aaccgacagc gcgaacatca gataaaacgc cggcccgaag
     7621 cgaacctttg ccacagacga gagcttgata tacgccacca gagtggaaac aaaaaacaca
     7681 tccaccatca tcgcctgcct caagcgcacc atcacacgcg ttgccaaacg cagcgcagga
     7741 tacgcctgtt tccgtatcag cgcggcatag acatacaggc acagcagcag aaacagaacc
     7801 ggcgcgccga aagtcagcac aaacatcact tcggccaaaa aaccataatc ctgaaacacc
     7861 atcaggcgca tcatctcggg cagcgaaagg acggatgccg cacccggtat cccgacctcg
     7921 atatacgtca tactgtaagc aaacgccatc aaaatcagcg aagccgccgc ataggcgggc
     7981 ggaccggaaa aagggtggct gcccaccctg aagagtttgt gtccgcaacg gggacagaac
     8041 gccgcctctc ccttgtccaa ctggggcacg tctgtacggc agccgcattc cgggcaatct
     8101 accgtatgcg gcggcagggc ttcgtcgcgg cagttgccgc gaaggctgtg ggggatattt
     8161 tcggcaaacg gcttcatcgg gaaagtggca aatataaatg gcggcattat aaaggaaaaa
     8221 aagcgataca cccaatcagg atgcaaaacc ggcaggttcg gcagtatgaa caaacccttg
     8281 gtgtccggca aaaatatttt ttgagcggct gcccctccgg ccgacaagcc tatttgataa
     8341 aaccccgatt ttgtaaataa tcataaaata cccgcttaaa aatataacgc aaatgatatt
     8401 ttttcgtaaa atatgtgata ataaatttcg tttatgattc tttattattt ttgtatgcgg
     8461 gcaagtccgg attttgcccg caccgcatcc ttatccctta ccgacaaagg agaacttatg
     8521 agcgaaaccg aaaaccaagc cctgacgttt gccaaacgcc tgaaggcgga taccacggcg
     8581 gttcacgaca gcgtggataa cctcgttatg tctgtccaac cgtttgtcag caaagaaaac
     8641 tacatcaaat ttttgaaact gcaatccgtg ttccacaaag cggttgatca tatctataaa
     8701 gaccccgagt tgaacaaagc catttccgag ctggaataca tggcgcgata cgatgccgta
     8761 acgcaagact tgaaagactt gggcgaagag ccttacaaat tcgacaaaga attgccacac
     8821 gaaaccggca acaaagccgt cggctggctt tattgcgccg aaggatccaa tttgggcgcg
     8881 gcatttttgt tcaaacacgc gcaaaagctg gattacaccg gcgaacacgg cgcgcgccac
     8941 ctcgctccgc atccggacgg gcgcggcaaa cactggcgcg ccttcgtcga gcacctgaat
     9001 gccctgaact taactccgga agcagaagca gaagccattc aaggcgcgca ggaagcgttt
     9061 gctttctata aagtgattct gcgtgaaacc ttcggtttgc cggaaggaac agaagccccc
     9121 gaaggcatga tgccgcacag acattaaaaa tggggctgta ctagattatc cctaaattcc
     9181 acaccgatcc cgcaggattt ttagctgccg gggcggtgtg ccg
//
LOCUS       DAWTQS010000072         8351 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.072, whole genome shotgun sequence.
ACCESSION   DAWTQS010000072 DAWTQS010000000
VERSION     DAWTQS010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8351
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.072"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            182..466
                     /locus_tag="WNA25_001803"
     CDS             182..466
                     /locus_tag="WNA25_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFA9824500.1"
                     /translation="MQPAVYILASQRNGTLYIGVTSDLVQRIYQHREHLIEGFTSRYN
                     VTMPVWYELHPTMESAITREKQLKKWNRAWKLQLIEENNVSWRDLWFDII"
     gene            complement(1020..3170)
                     /gene="yccS"
                     /locus_tag="WNA25_001804"
     CDS             complement(1020..3170)
                     /gene="yccS"
                     /locus_tag="WNA25_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HFA9824501.1"
                     /translation="MKTPLLKPLLITSLPVFASVFTAASIVWQLGEPKLAMPFVLGII
                     AGGLVDLDNRLTGRLKNIIATVALFTLSSLTAQSTLGTGLPFILAMTLMTFGFTILGA
                     VGLKYRTFAFGALAVATYTTLTYTPETYWLTNPFMILCGTVLYSTAIILFQIILPHRP
                     VQESVANAYEALGGYLEAKADFFDPDEAAWIGNRHIDLAMSNTGVITAFNQCRSALFY
                     RLRGKHRHPRTAKMLRYYFAAQDIHERISSAHVDYQEMSEKFKNTDIIFRIHRLLEMQ
                     GQACRNTAQALRASKDYVYSKRLGRAIEGCRQSLRLLSDGNDSPDIRHLSRLLDNLGS
                     VDQQFRQLRHSDSPAENDRMGDTRIAALETGSFKNTWQAIRPQLNLESGVFRHAVRLS
                     LVVAAACTIVEALNLNLGYWILLTALFVCQPNYTATKSRVYQRIAGTVLGVIVGSLVP
                     YFTPSVETKLWIVIAGTTLFFMTRTYKYSFSTFFITIQALTSLSLAGLDVYAAMPVRI
                     IDTIIGASLAWAAVSYLWPDWKYLTLERTAALAVCSSGTYLQKIAERLKTGETGDDIE
                     YRITRRRAHEHTAALSSTLSDMSSEPAKFADSLQPGFTLLKTGYALTGYISALGAYRS
                     EMHEECSPDFTAQFHLAAEHTAHIFQHLPDMGPDDFQTALDTLRGELGTLRTRSSGTQ
                     SHILLQQLQLIARQLEPYYRAYRQIPHRQPQNAA"
     gene            complement(3287..4513)
                     /locus_tag="WNA25_001805"
     CDS             complement(3287..4513)
                     /locus_tag="WNA25_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HFA9824502.1"
                     /translation="MNTDNLHDILDETVQVYSQKNKAESETPAEIGAHFHPLLDRLCE
                     TAEAQNASDILISKGFPPSLKINSALTPQPQKALTGEETAAIAASTMNAEQSEIFRRD
                     GEINYSVQSRSGTRYRANAYYSQGSAGLVLRRINHVIPQMRELGLPEKLKDLAVAPRG
                     LLIIVGPTGSGKSTTMATMLEHRNKTLPGHIVTIEDPIEFIYKPRRCIFTQREIGVDT
                     INWQTAVQNAMRQSPDVVCIGEVRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERI
                     LNFYPKEQHNQILIDIALNLTGIICQRLALKKDKTGRTAVVDLLINTPAIQDFILKGD
                     LMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGK
                     TPELLYDRVNGLNLIS"
     gene            complement(4677..5720)
                     /locus_tag="WNA25_001806"
     CDS             complement(4677..5720)
                     /locus_tag="WNA25_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFA9824503.1"
                     /translation="MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMRRINLPEMSA
                     EEVGNMVTSVMNDHQRKIYQQNLEVDFSFELPNVARFRVNAFNTGRGPAAVFRTIPST
                     VLSLEELKAPSIFQKIAESPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
                     PIEFVHQSKKSLINQRELHQHTLSFANALSSALREDPDVILVGEMRDPETIGLALTAA
                     ETGHLVFGTLHTTGAAKTVDRIVDVFPAGEKEMVRSMLSESLTAVISQNLLKTHDGNG
                     RVASHEILIANPAVRNLIRENKITQINSVLQTGQASGMQTMDQSLQSLVRQGLIAPEA
                     ARRRAQNSESMSF"
     gene            5840..6526
                     /locus_tag="WNA25_001807"
     CDS             5840..6526
                     /locus_tag="WNA25_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFA9824504.1"
                     /translation="MTVLQERYREVSDRIGKLVLQAGREPHSVSLIAVSKTFPSDGIR
                     EVYAAGQRDFGENYIQEWYGKTEELADLTDIVWHVIGDVQSNKTKFVAERAHWVHTVC
                     RLKTAVRLSRQRRSLMPPLQVCVEVNIAGEAAKHGVAPEEAVALAVEVAKLPNLVVRG
                     LMCVAKANSSETELKTQFQTMQKLLADLNAAGVKADVLSMGMSDDMPAAIECGATHVR
                     IGSAIFGKRG"
     gene            6526..6933
                     /locus_tag="WNA25_001808"
     CDS             6526..6933
                     /locus_tag="WNA25_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFA9824505.1"
                     /translation="MEIRVIKYTATAALFAFMVAGCRLAGWYECSSLSGWCKPRKPAA
                     IDFWDIGGESPLSLEDYEIPLSDGNRSVRANEYESAQKSYFYRKIGKFEACGLDWRTR
                     DGKPLVERFKQEGFDCLEKQGLRRNGLSERVRW"
     gene            6982..7773
                     /locus_tag="WNA25_001809"
     CDS             6982..7773
                     /locus_tag="WNA25_001809"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFA9824506.1"
                     /translation="MNVYFLGGGNMAAAVAGGLVKQGGCRIHIANRGAEKRERLGKEL
                     GVETSATLPELHSDDVLILAVKPQDMEAACKNIRTNGALVLSVAAGLSVGTLSRYLGG
                     SRRIVRVMPNTPGKIGLGVSGMYAEAEVSETDRRIADRIMKSVGLTVWLEDEAQMHSI
                     TGISGSGPAYVFYLLDALQNAAIRQGFDMAEARALSLATFKGAVALAEQTGEDFEKLQ
                     KNVTSKGGTTHEAVEAFRRHRVAEAISEGVCACVCRSQEMERQYQ"
     gene            7922..8338
                     /gene="dksA"
                     /locus_tag="WNA25_001810"
     CDS             7922..8338
                     /gene="dksA"
                     /locus_tag="WNA25_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFA9824507.1"
                     /translation="MVKLTEQDILNWIGPEDDYMNDDHLAFFRELLVKMQDELIENAS
                     VTTGHLQEHESAPDPADRATQEEEYALELRTRDRERKLLSKIQATIRNIDEGDYGFCA
                     DTGEPIGLKRLLARPTATLSVEAQERRERMKKQFAD"
BASE COUNT         1749 a         1816 c         2743 g         2043 t
ORIGIN      
        1 cgtgggacaa cagcaatatt caaaggttag ctgaagcttt agagattctg gattcccgct
       61 ttcgcgggaa tgacgaagag ttgcgggaat gatggaaagc tatggggata acgaagggtt
      121 aaagtaatca ccggatggtg ttcgcgggaa tataaattta aataattcaa aagggtatta
      181 tatgcagcct gcggtttata ttttagcaag ccaacgtaat ggcacgttat acattggcgt
      241 tacatctgat ttggtgcaac gtatttacca acatagggag catttgattg aaggatttac
      301 atcacggtac aacgttacta tgccggtttg gtatgaactg catcctacga tggagagcgc
      361 aattactcgg gaaaaacagt tgaagaaatg gaacagggct tggaaattgc aactgattga
      421 agaaaataat gtttcttggc gggatttatg gtttgatatt atttagcccg tcattcccgc
      481 tcaggcggga atccggcttg ttcggtttcg gttttttttg aggtttcggg caacttctaa
      541 accgtcattc ccgcgcaggc gggaatctag aacgtaaaat ctaaagaaac cgttttaccc
      601 gataagtttc tgtgccgaca gacctagatt cccactttcg tgggaatgac gggattagag
      661 tttcaaaatt tattctaaat agctgaaact caacgcactg gattcccgcc tgcgcgggaa
      721 tgacgggatt tgagattgcg gcatttatcg gaaaaaacag aaaccgctcc gccgtcattc
      781 ccgcgcaggc gggaatctag acattcaatg ctaaggcaat ttatcgggaa tgactgaaac
      841 tcaaaaaacc ggattcccac tttcgtggga atgacgggat tttaggtttc tgtttttggt
      901 tttctgtttt tgagggaatg acggttcagt tgctacgcat ttaccctgcg caaagcctta
      961 tccactatct tgcaacctgc ctgacaatcc gccccccttt acaaaatgcc gaaatttttt
     1021 caggctgcgt tttggggctg cctgtgcgga atttgtcggt aggcgcggta gtagggttcg
     1081 agctgccggg cgatgagttg gagctgttgg aggaggatgt ggctttgtgt tccgctgctg
     1141 cgggtgcgga gggtgccgag ttcgccgcgc agtgtatcca atgccgtctg aaagtcgtcg
     1201 ggtcccatgt cgggcaggtg ttggaagatg tgggcggtgt gttcggcggc aaggtggaac
     1261 tgtgcggtaa agtcggggct gcattcttcg tgcatttcgc tgcggtatgc gccgagggcg
     1321 gagatgtagc cggtcagggc gtagccggtt ttgagcaggg taaagccggg ttgcaggctg
     1381 tcggcgaatt ttgcgggttc gctgctcatg tcggaaaggg tgctgctgag ggcggcggtg
     1441 tgttcgtggg cgcggcggcg ggtgatgcgg tattctatgt cgtcgccggt ttcgccggtt
     1501 ttgaggcgtt cggcaatttt ttggaggtat gtgccgctgc tgcatacggc aagggcggcg
     1561 gtgcgttcga gcgtgaggta tttccagtct ggccacaggt agctgaccgc cgcccaggca
     1621 agggatgcgc cgataatggt gtcgatgatg cgcacgggca tggcggcgta tacgtccaaa
     1681 cctgcgaggg agaggctggt cagtgcctga atggtgatga agaaggtgga aaaactgtat
     1741 ttgtaggtgc gggtcatgaa aaagagggtg gtaccggcga tgacgatcca gagtttggtt
     1801 tcgacggagg gggtgaagta ggggacgagc gagccgacga ttacgccgag tacggtgccg
     1861 gcgatgcgtt ggtacacgcg gcttttggtg gcggtgtagt tgggttggca gacgaaaagg
     1921 gcggtcagca gtatccagta gccgaggttg aggttgaggg cttcgacgat ggtgcaggcg
     1981 gcggcaacga cgagggacag gcggacggca tggcggaata cgcctgattc gaggttcagc
     2041 tgcggacgga ttgcctgcca ggtgtttttg aagctgccgg tttcgagggc ggcgatgcgg
     2101 gtgtcgccca tgcggtcgtt ttcggcgggg gagtcgctgt gtcggagttg gcggaactgc
     2161 tggtcgacgc tgccgaggtt gtcgagaagg cggctcaggt ggcggatgtc gggactgtcg
     2221 ttgccgtctg aaaggaggcg cagcgattgg cggcagcctt cgatggcgcg tccgaggcgt
     2281 ttgctgtaaa cgtagtcttt gcttgcgcgc agggcttgtg cggtgttgcg gcaggcttgt
     2341 ccctgcattt cgagcaggcg gtggatgcgg aagatgatgt cggtgttttt gaatttttcg
     2401 gacatctctt ggtagtcgac gtgggcggag ctgatgcgtt cgtggatgtc ttgggcggcg
     2461 aagtagtagc gcagcatttt ggcggtgcgc gggtggcggt gtttgccgcg caaacggtaa
     2521 aacagggcgg aacggcattg gttgaaggcg gtgatgacgc cggtgttgct catggcgagg
     2581 tcgatgtggc ggttgcctat ccaggctgcc tcatcggggt cgaagaagtc ggctttggct
     2641 tcgaggtagc cgccgagtgc ttcgtaggcg ttggcgacgc tttcttggac ggggcggtgg
     2701 ggcaggatga tttggaacag gatgatggcg gtgctgtaca gtacggtgcc gcataaaatc
     2761 atgaaggggt tggtcagcca gtaggtttcg ggggtgtagg taagcgtggt gtaggtggcg
     2821 acggcgagtg cgccgaaggc gaaggtgcgg tatttcagcc cgaccgcgcc taaaatggta
     2881 aagccgaagg tcatcagggt catggcgagg atgaagggca gccctgtgcc gagggtgctt
     2941 tgcgccgtga gcgaggagag ggtaaacagg gcgacggtgg cgatgatgtt tttcagccgt
     3001 ccggtcaggc ggttgtccaa atcgaccagg ccgccggcga tgatgccgag tacgaagggc
     3061 atggcgagct tgggttcgcc tagctgccag acgatggagg cggcggtaaa gacactggcg
     3121 aaaacgggaa gcgaggtaat gagcagaggc ttgaggagtg gggttttcat ggttttaccg
     3181 gtttattgtt atgaagtgaa taaagtgtgg cacatgaatg gggcggataa aatcatgccg
     3241 tctgaaaacg gggatgcggt tttcagacgg cattgggttt tgcggatcag gaaatgaggt
     3301 tgagaccgtt gaccctgtcg taaaggagtt cgggcgtttt gccttctttg tgcagttgga
     3361 tgtgcaatcg caggttgttg gcggaaacgg actggcgcag ggcttcttcg taactgatga
     3421 tgccgtgacg gtacagttcg aaaaggtttt ggtccatcgt ctgcattccg tcggttttgg
     3481 cggtttccat gattttactg atgttcatca ggtcgccctt caggatgaag tcttggatgg
     3541 cgggcgtgtt gatgagcaag tcgacaaccg ccgtcctgcc cgttttgtct tttttgaggg
     3601 cgaggcgttg gcagatgatg ccggtcagat tgagggcgat gtcaatcagt atttggttgt
     3661 gctgttcttt ggggtagaag ttgagtatgc gttcgagcga ctgcggcgcg gtgttggcgt
     3721 ggagcgtaaa aatgcacagg tggccggttt gggcgagctg catcgcgtat tccatacttt
     3781 ccctgctgcg gacttcgccg atacagacca cgtctgggga ttggcgcata gcgttttgta
     3841 ccgccgtctg ccagtttatg gtgtcgacgc cgatttcgcg ctgggtaaag atgcagcggc
     3901 gcggtttgta gataaactca atcgggtctt cgatggtaac gatatggccg ggcagggttt
     3961 tgttgcggtg ttcgagcata gtcgccatcg tggtggattt gcccgaaccg gtaggcccga
     4021 cgataatcag cagcccgcgc ggtgcgacgg cgaggtcttt gagtttttcg ggcaagccca
     4081 attcccgcat ttgcgggatg acgtggttga tgcgccgcaa aaccaaacct gcgctgcctt
     4141 ggctgtagta ggcgttggcg cggtagcgcg tgccgctgcg cgattggacg gagtagttga
     4201 tttcgccgtc gcgccggaat atttccgatt gttcggcgtt catcgtcgat gcggcgatgg
     4261 cggcggtttc ctcgcccgtc agagcctttt gcggctgcgg ggttaatgcg ctgttgattt
     4321 tcaacgaggg cgggaatcct ttgctgataa ggatgtcgga cgcgttttgt gcttctgcgg
     4381 tttcgcacag gcggtcgagc agcgggtgga agtgtgcgcc gatttcggcc ggggtttcgg
     4441 attcggcttt gtttttttga gaatacactt gaaccgtttc gtccaagatg tcgtgcaggt
     4501 tatcggtatt catcgttagc ttcttttcgg tttaagcctt gcagtttgcg gcggcaggtt
     4561 tcaacaggaa ggcggacgct tcctgttcgg aagggtatgc cgggcgggat gccgcgtccc
     4621 gcccggcgtg tttgcgcctt gttttcccgc cggtatggcc ggaaggcggt tgtgtgtcag
     4681 aaactcatac tttcgctgtt ttgcgcgcgt ctgcgtgcgg cttccggtgc gatcagccct
     4741 tggcgcacca gcgattgcag cgattggtcc atcgtctgca taccgctcgc ctgcccggtt
     4801 tgcaggacgg agttaatctg cgtgattttg ttttcgcgga tgaggttgcg gacggcgggg
     4861 ttggcaatca ggatttcgtg cgaggcgaca cggccgttgc cgtcgtgcgt tttcagcagg
     4921 ttttgggaga tgacggcggt cagcgattcg gacagcatgg aacgcaccat ttctttctct
     4981 ccggcgggga atacgtccac gatacggtcg acggtttttg ccgcgccggt cgtgtgcagc
     5041 gtgccgaaaa ccaagtgtcc ggtttcggcg gcggtcagtg ccaagccgat ggtttcgggg
     5101 tcgcgcatct ctccgacaag gataacgtcg gggtcttcgc gcaatgcgga actcagcgcg
     5161 ttggcgaagc tgagggtgtg ctggtgcagc tcgcgttggt taatcaggga ttttttgctt
     5221 tggtggacga attcgatcgg gtcttcgatg gtcaggatgt gtgccggctg ggtttcgttg
     5281 atgtagttga tcatcgcggc aagcgtggtc gatttgcccg aaccggtagg gccggtaacc
     5341 aataccatgc cgcgcggcga ttctgcgatt ttttggaaaa tgctcggggc tttcaattct
     5401 tccagcgata agacggtgct gggaatggtg cggaatacgg cggcggggcc gcggccggtg
     5461 ttgaaggcgt tgacgcggaa tcgggcgacg ttgggcagtt cgaacgagaa gtcgacttcc
     5521 aagttttgct ggtagatttt ccgctggtgg tcgttcatca ccgaagttac catattgccg
     5581 acctcttccg cgctcatttc gggaaggttg atgcgccgca tgtcgccgtg aacccgaatc
     5641 ataggggata tgcccgaact caggtgaagg tcggatgctt tgtttttagc gccgaaggcg
     5701 agtaagtcgg taatctgcat aatgcggctc tgtttagtat aatgtttcga ttggttggaa
     5761 tgattctaac aaccttgatt ataccgccct gactggaggg gtttcaactg tttaatcatt
     5821 tttaattagg ggatgatcta tgacggtgtt gcaagaacgt tatcgtgagg tgtccgaccg
     5881 tatcggaaaa ttggttctgc aggcgggcag ggagccgcat tccgtcagcc tgattgccgt
     5941 cagcaagacc ttcccttcag acggcatccg cgaagtttac gctgccggac agcgtgattt
     6001 cggcgagaac tatattcagg agtggtacgg caaaacggag gaattggcgg atttgaccga
     6061 catcgtgtgg cacgtcatcg gagatgtgca gtccaacaaa accaaatttg tcgccgaacg
     6121 cgcgcattgg gtgcataccg tatgccgtct gaagaccgcc gtccggctga gcaggcagcg
     6181 tcgctcctta atgccgccct tgcaggtgtg tgtcgaggta aacattgcgg gcgaggcggc
     6241 aaaacacggt gtcgcgcccg aagaagcggt cgcgcttgcc gtggaagtgg cgaagctgcc
     6301 caaccttgtc gtgcgcggac tgatgtgcgt tgccaaagcc aacagcagtg aaacggagtt
     6361 gaaaactcag tttcagacca tgcaaaagct gcttgccgac ctcaatgcgg ctggcgttaa
     6421 ggcggacgtg ctgtctatgg ggatgtcgga cgatatgcct gccgccattg agtgcggtgc
     6481 gacacacgtc cgtatcggca gcgcgatttt cgggaaaagg ggctgatgga aattcgggta
     6541 ataaaatata cggcaacggc tgcgttgttt gcatttatgg ttgcaggctg ccggctggcg
     6601 gggtggtatg agtgttcgtc cttgtccggc tggtgtaagc cgagaaaacc tgccgccatc
     6661 gatttttggg atattggcgg cgagagtccg ctgtctttag aggactacga gataccgctt
     6721 tcagacggca atcgttccgt cagggcaaac gaatatgaat ccgcgcaaaa atcttacttt
     6781 tataggaaaa tagggaagtt tgaagcctgc gggttggatt ggcgtacgcg tgacggcaaa
     6841 cctttggttg agaggttcaa acaggaaggt ttcgactgtt tggaaaagca ggggttgcgg
     6901 cgcaacggcc tgtccgagcg cgtccgatgg taaaaaattg ggaatgaatt tagtaaggta
     6961 attttgaata gggtagaaat aatgaatgtt tattttctcg gcggcggaaa tatggcggct
     7021 gccgttgcgg gcggattggt caaacaaggg ggatgccgca tccatatagc caaccgtggt
     7081 gcggaaaagc gcgaacgttt gggaaaagag ttgggggtcg aaacttcggc aaccctgccg
     7141 gagcttcatt ccgacgatgt tttaatcctt gccgtcaaac cgcaggatat ggaagccgcg
     7201 tgcaaaaata tccgcaccaa cggtgcattg gtgctttctg tcgcagccgg attgtcggtc
     7261 ggtacgctca gccgttacct cgggggaagc cgccgcattg tccgggttat gccgaataca
     7321 cccggaaaaa tcgggctggg cgtatccggt atgtatgccg aagcggaagt atcggaaaca
     7381 gaccgcagga ttgccgatcg aatcatgaaa tcagtcggtt tgaccgtttg gttggaagat
     7441 gaagcgcaaa tgcacagcat taccggcatc agcggcagcg ggccggctta tgtgttttat
     7501 ctgctggacg cattgcaaaa tgccgccatc cgacaagggt ttgatatggc agaagcacgc
     7561 gcgctcagtc tggcaacgtt taaaggagcg gttgcccttg ccgagcagac gggtgaagat
     7621 ttcgagaagc ttcaaaaaaa tgtaacgtca aaaggcggga caacccacga agccgtggaa
     7681 gccttcagac ggcatcgtgt cgccgaagcc ataagtgagg gcgtttgtgc ctgtgtgtgc
     7741 cgttcgcagg aaatggaacg gcaatatcaa taatgtaaag aaaataaaaa aaaccaatcc
     7801 aaaacgtgtt atgatgcgcg ttttcaaaaa cgccttaggc aataagcctt ataaaaatca
     7861 aaggaataaa gccgctttgt agtgctttgt ttttttgcgg tgaaccgaga gggcatacat
     7921 tatggtaaag ctgacagaac aagatatttt gaattggatt gggccggaag acgattatat
     7981 gaatgacgac catttggctt ttttccgcga attgctggta aaaatgcaag acgaactcat
     8041 cgaaaacgcc tccgttacaa cagggcatct ccaagaacac gaatcagccc ccgatcctgc
     8101 cgaccgtgcc acacaggaag aagagtacgc attggaactc cgtacccggg atcgggaacg
     8161 aaaacttctc agtaaaatac aggcgaccat ccgcaatatt gatgaagggg actatggatt
     8221 ctgtgccgat acgggagaac ctatcggtct gaagcggctg ctggcacgcc cgacagccac
     8281 tttatccgtt gaggcccaag aacgccgaga gaggatgaag aaacagtttg ccgactgatg
     8341 gaggcaaaca a
//
LOCUS       DAWTQS010000073         8099 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.073, whole genome shotgun sequence.
ACCESSION   DAWTQS010000073 DAWTQS010000000
VERSION     DAWTQS010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8099
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.073"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            332..1009
                     /locus_tag="WNA25_001811"
     CDS             332..1009
                     /locus_tag="WNA25_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFA9824508.1"
                     /translation="MTFFKPFTVVLTASALALSGCVADPVTGQQSPNKSAMYGLGGAA
                     VCGIVGALTHSGKGARNSALACGAIGAGVGGYMDYQEQRLRQNLAGTQIEIQRQGNQI
                     RLVMPESVTFATGSAALGGSAQYALNTAAQTLVQYPDTTLTINGHTDNTGSDAVNNPL
                     SQHRAQAVAYYLQTRGVAASRLTVYGYGSHMPVASNATVEGRAQNRRVEILINPDQRA
                     VNAARHM"
     gene            complement(1283..2053)
                     /gene="xth"
                     /locus_tag="WNA25_001812"
     CDS             complement(1283..2053)
                     /gene="xth"
                     /locus_tag="WNA25_001812"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFA9824509.1"
                     /translation="MKITTWNVNSLNVRLPQVQNLLADNPPDILVLQELKLDQDKFPA
                     AALQMMGWHCVWSGQKTYNGVAIVSRSVPQDVHFGLPSLPDDPQRRVIAATVGGVRVI
                     NVYCVNGEALDSPKFKYKEQWFAALTEFVRDEMTRHGKLVLLGDFNIAPADADCYDPE
                     KWYEKIHCSSVERQWFQNLLDLGLTDSLRQVHPEGAFYTWFDYRGAMFQRKLGLRIDH
                     ILVTSEMAAVLKDVRIDLETRALERPSDHAPVAAEFDL"
     gene            complement(2113..2394)
                     /locus_tag="WNA25_001813"
     CDS             complement(2113..2394)
                     /locus_tag="WNA25_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFA9824510.1"
                     /translation="MNTIPLHTILKLMAHPERMAILIQLLDSERNIVELAKSLSLSAT
                     AVSCHLNRLRAGGLVDFTRYHRIIEYRLVSEDAATILRTIRDLENKRAA"
     gene            2647..2898
                     /locus_tag="WNA25_001814"
     CDS             2647..2898
                     /locus_tag="WNA25_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824511.1"
                     /translation="MDIFRESRPFCATGCPTPTSGSAFHHRRRRYGQDRIGRAFLTMH
                     RILDKENQIHTDMALALPAVVKDAVAIWADMDALDRCSG"
     gene            complement(3129..4010)
                     /gene="nadC"
                     /locus_tag="WNA25_001815"
     CDS             complement(3129..4010)
                     /gene="nadC"
                     /locus_tag="WNA25_001815"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFA9824512.1"
                     /translation="MPSENPLFALPDTLLRPMVEQALSEDLGRRGDITSAAVIAPDKT
                     AKLFLVSREDGVIAGMDLARLAFQTMDPCVRFQAEIQDGQAVRAGQTLAAVEGNARAL
                     LAAERTALNYLTHLSGIATATARAVAEVAEYGTDIVCSRKTIPLLRVLQKYAVRAGGG
                     ANHRMGLDDAVLIKDNHLAYCGSIAQAVRQAKQAVGPLTCVEIEVDTLAQLDEAIAAG
                     AERILLDNMDDETLKEAANRCHTQTAHPHTVYCEASGGIGFDRLKRVAQTGVDGIALG
                     YLTHSSRSLDIGLDFVA"
     gene            complement(4086..5024)
                     /locus_tag="WNA25_001816"
     CDS             complement(4086..5024)
                     /locus_tag="WNA25_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824513.1"
                     /translation="MDAYPEAEAPLQSIVELVPVLIAVTDGGLRVLTVAQGTLLPNGP
                     LSPLRNSLQAGVKLWVAKQTSQPMGYVEQLYTFVDTHRRNEHGMPVLYVSYLGLVREA
                     ADSILHPDAKWQDCYGYFPWEDLRTDGGQRDAVVSRLRIWANSADTEEVRQKRLKHIH
                     LCWGVEPENWSEEYVLQRYEMLYESGLIAEAAEPQANFDFALTGQTMRHDHRRVLATA
                     LSRLRAKIKYRPVIFELMPPGFTLLQLQNSVEAISGRLLHKQNFRRQIQQQNLIEPSD
                     TGVSGSKGRPAQLYRFRDNVLSDRLISDIGLPLGSR"
     gene            5226..6338
                     /gene="nadA"
                     /locus_tag="WNA25_001817"
     CDS             5226..6338
                     /gene="nadA"
                     /locus_tag="WNA25_001817"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFA9824514.1"
                     /translation="MQTAARRSFDYDMPLIQTPTSACQIRQAWAKVADTPDHETAGRL
                     KDEIKALLKRKNAVLVAHYYVDPLIQDLALETGGCVGDSLEMARFGAEHEAGTLVVAG
                     VRFMGESAKILCPEKTVLMPDLEAECSLDLGCPEEAFSAFCDQHPDRTVAVYANTSAA
                     VKARADWVVTSSVALEIVSYLKSRGEKLIWGPDRHLGDYIRRETGADMLLWQGSCIVH
                     NEFKGQELAALKAEHPDAVVLVHPESPQSVIELGDVVGSTSKLLKAAVSRPEKKFIVA
                     TDLGILHEMQKQAPDKEFIAAPTAGNGGSCKSCAFCPWMAMNSLGGIKHALTGGRNEI
                     LLDRKLGEAAKLPLQRMLDFAAGLKRGDVFNGMGPA"
     gene            6390..7898
                     /gene="nadB"
                     /locus_tag="WNA25_001818"
     CDS             6390..7898
                     /gene="nadB"
                     /locus_tag="WNA25_001818"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFA9824515.1"
                     /translation="MQTDCDVLIAGNGLAALTLALSLPESFRIVILCKNRLDDTASRH
                     AQGGIAAAWSGEDDIGKHVADTLEAGAGLCDEAAVRTILSQGKPAIEWLLAQGVAFDR
                     NHNDLHLTREGGHTCRRIAHVADYTGEAVMQSLIIQIRRRPNIRVYERQMALDVQTES
                     GAACGLTVLDCRTQETYRIRARHTVLAGGGLGQIYAATTTPPECTGDAIAMAIRAGCA
                     IENLEFIQFHPTGLARPSENGRTFLISEAVRGEGGILTNQSGERFMPHYDRRAELAPR
                     DIVARAIAAETAKQTQDFVSLDISRQPAAFVRRHFPSIHRHCLSQCGLDITRQAIPVR
                     PVQHYTCGGIQTDPSGRTSLPQLYALGETACTGLHGANRLASNSLLECVVTARLAAQG
                     IADGQAFQIEPFQRPSESPSAETDIFSNDLQNTFSRPVLQTFNQRHLGILRNDTDLHR
                     AIDQLRLWKQNQAEPHTASEYENRNLLECSLAVAQAAYRRRQNIGAHFNSDC"
BASE COUNT         1765 a         2386 c         2267 g         1681 t
ORIGIN      
        1 tcctaaaccc accatccgcg cctgccgcac gtacacgtcg acaaaccgaa aatatggctg
       61 ctcggcggca tcgtcggcgc gtggggctgc cataaatcgg acaccatttc tccctgcccg
      121 tttcggcatt attgccgata ccgggtgcag gctcggtcgg ctacgatgtg aaggtacgtt
      181 tttttgcgga tgtggctgcg gtttcggcgc aaatcacgat aaacccgcgt tgtttcggga
      241 aaaatcgggt aatttcaggt taattgcact ttttcagacg gcatgattgc ttatagttgt
      301 acggtttgac atatgcacgg aaggaaacgc catgactttc ttcaaaccct ttaccgtcgt
      361 gctgaccgca tccgcactcg cgctttccgg ctgcgttgcc gaccccgtaa ccggacagca
      421 gtccccaaac aaatccgcca tgtacggttt gggtggcgcg gcagtgtgcg gcatcgtcgg
      481 cgcactgacc cacagcggca aaggcgcacg caattccgcg cttgcctgcg gcgcaatcgg
      541 cgcaggcgtg ggcggctata tggactacca agagcagcgt ttgcgccaaa accttgccgg
      601 cacgcaaatc gaaatccaac gccaaggcaa ccaaatcagg ctggtgatgc ccgaaagcgt
      661 taccttcgcc accggcagcg cggcgttggg cggcagtgcg caatacgccc tgaacactgc
      721 cgcacagacg ctggtgcagt atcccgacac gacgctgacc atcaacgggc acaccgacaa
      781 cacaggttcc gatgcagtca acaatccgct ttcgcaacac cgcgcccaag cggttgccta
      841 ctatctgcag acgcgcggcg tggcggcttc gcgcctgacg gtttacggct acggttcgca
      901 tatgccggtc gcgtccaacg ctacggttga aggccgcgcg caaaaccgcc gcgtcgaaat
      961 cctcatcaac cccgaccaac gcgccgtcaa cgccgcacgg cacatgtaac gcgccctgca
     1021 acgccaatgc cgtctgaatg tttttcagac ggcatttccg tattcgcaag cacagccgta
     1081 ccccgactgt aatgctatgg ataacgtctc gccgtttgcc gggaaaccgc agattgcagc
     1141 cggtggcgtg caagtgggcc caaccttgca gacgttaccg ccataccgcg atacccctgt
     1201 aacctcatcg tttaaacaga tattcaatca aaatgccgtc tgaaaccctg cgaagtttca
     1261 gacggcattt gcctttcccg aattacaaat caaattctgc cgccacgggc gcgtggtcgc
     1321 tcggacgctc cagcgcgcgc gtctccaaat cgatgcggac atccttcaaa acggccgcca
     1381 tttcggatgt aaccaatata tggtcgatac gcaggcccag tttgcgttgg aacatcgcgc
     1441 cgcgatagtc gaaccaggta tagaacgcgc cttcggggtg gacttggcgc aggctgtcgg
     1501 tcaatcccaa atccagcaga ttttgaaacc actgccgttc gacggacgaa cagtggattt
     1561 tttcatacca tttttcaggg tcgtaacagt ccgcatcggc aggcgcaata ttgaaatcgc
     1621 ccagcagtac cagtttgccg tggcgggtca tttcgtcgcg gacaaactcc gtcagtgcgg
     1681 caaaccactg ttccttatat ttgaatttgg ggctgtccaa agcctcgccg ttgacgcaat
     1741 agacattgat gacgcgcacg ccgcctacgg tcgccgcaat cacgcggcgt tgcgggtcat
     1801 ccggcaggga aggcaaaccg aaatgcacgt cctgcggcac gctgcggctg acgattgcca
     1861 caccgttgta ggttttctgc ccgctccaaa cacagtgcca gcccatcatc tgcaaggcgg
     1921 cggccggaaa tttgtcctga tcgagtttga gttcctgcaa aaccaaaata tcgggcggat
     1981 tgtcggcaag caggttttgc acctgcggca gccgcacatt gagcgaattg acgttccaag
     2041 tggtgatttt cataacattt ccgaaaaatg ccgtctgaaa cgttcagacg gcaaaagaaa
     2101 aggattctaa cactatgccg cgcgtttgtt ttccaaatcg cggattgtcc gcagaatcgt
     2161 tgccgcgtct tcggaaacca ggcggtattc gataatgcgg tggtaacgcg taaagtccac
     2221 cagaccgccc gccctcaggc ggttcaaatg acaggaaacc gcggtagccg acagggataa
     2281 ggattttgcc agttcgacga tattgcgttc gctgtccaac agttggatca gtatcgccat
     2341 acgttcggga tgcgccataa gtttgagtat ggtgtggagc ggtattgtgt tcatcggtat
     2401 tcctaaaacc ttttccgccc gccaagcgga ttgacgggcg gcagatatgg atgattttga
     2461 ttatctttat aaattttatt ataaatcaat tgtttatttc tcattgtaca ggattgtatg
     2521 ttccttttcc cactattttt gcaaacaagc gtaaagacag gctttcagac ggcataaaac
     2581 tgccgcccct accctgcctc accctgttgc cgaacggaat catccgtatt ttttaagaaa
     2641 accattatgg atattttccg tgaatcaagg ccgttctgcg cgacaggctg tccaacgcca
     2701 acatccggtt cggcatttca tcatcgccgc cgacgatacg ggcaagaccg catcggtcgt
     2761 gccttcctaa cgatgcaccg catcctcgac aaggaaaacc agatacatac cgatatggct
     2821 ttagcgttgc cggcggtagt gaaagacgca gtggcgatat gggcagatat ggatgcattg
     2881 gataggtgtt ccggctgacc gctccgttac tgccgtatct ttcggctacc gttcatcaaa
     2941 tggttccaat gccgaaatgc ctaaatcgcg cctgcctgaa atgctgtttt ttctaaagca
     3001 tcacgacctt cacgaaaaat gccgtctgaa gaatatttca gacggcattc cctccctgag
     3061 gcaataaccg gattacggtt aagcggttcc ttacctgaca tatcagacag ccgcccgcac
     3121 cctgaaactc acgccacgaa atccaaacct atgtccaacg atcggctgct gtgggtcaga
     3181 tagccgaggg cgatgccgtc cacgccggtt tgcgccacgc gcttcaggcg gtcgaagccg
     3241 atgccgcccg atgcttcgca atagacggtg tgtgggtggg cggtttgtgt gtggcagcgg
     3301 tttgccgctt ctttcagggt ttcgtcgtcc atgttgtcca gcaaaatccg ttccgcgccc
     3361 gctgcgatgg cttcgtccag ttgtgccaac gtgtccactt cgatttccac gcaggtcaac
     3421 ggtccgaccg cctgttttgc ctgccgaacg gcttgggcga tgctgccgca ataggcgagg
     3481 tggttgtctt tgatgagcac ggcgtcgtcc aaacccatgc ggtggttcgc gccgccgccc
     3541 gccctgacgg cgtatttttg caggacgcgc agcaggggga tggttttgcg gctgcacacg
     3601 atgtctgtac cgtattcggc gacctcggca acggcacgag cggtggcggt ggcgatgccg
     3661 ctcaagtgcg tgaggtagtt gagcgcggtg cgttcggcgg cgagcagcgc gcgggcgttg
     3721 ccttcgacgg cagccagtgt ctgacctgcg cggacggctt gcccgtcttg gatttcggct
     3781 tggaagcgga cgcacggatc catcgtctga aaggcgaggc gcgccaagtc cataccggcg
     3841 ataacaccgt cttcgcggct gacgaggaaa agtttggcgg ttttgtcggg cgcgatgacg
     3901 gcggcggacg taatgtcgcc gcgcctgccc aagtcttcgc tcaaggcttg ttccaccatg
     3961 gggcgcaaca aggtgtcggg cagggcaaag agggggtttt cagacggcat ggggtttcct
     4021 ttgttaaaaa agattgatgt tgcaccgatg gggcaaaact tcatttgatg ccgtctgaaa
     4081 acgggctaac ggctgcccag cggcagtccg atgtccgaaa tcagcctgtc ggacaggacg
     4141 ttgtcgcgga agcggtaaag ctgcgcggga cggcctttgc tgcccgatac gccggtatcc
     4201 gacggctcga taaggttttg ctgctgaatc tggcggcgga agttttgctt gtgcagcaat
     4261 ctgccgctga tggcttcgac gctgttttgc agttgcagca gtgtaaatcc gggcggcatc
     4321 agttcgaaaa tcacggggcg gtatttgatt ttggcgcgca ggcgggacag ggcggtcgcc
     4381 aacacgcggc ggtggtcgtg gcgcatggtc tgccccgtga gcgcgaagtc gaagtttgcc
     4441 tgcggctcgg cggcctccgc tatcagaccg ctttcataca acatttcata gcgttgcaaa
     4501 acgtattctt ccgaccagtt ttccggttcg accccccaac acaaatgaat atgcttgagc
     4561 cgcttttggc ggacttcctc cgtgtccgcc gagtttgccc aaatgcgcag gcggctgacg
     4621 acggcgtcgc gctgcccgcc gtcggtgcgc aagtcttccc atgggaaata gccgtagcag
     4681 tcctgccatt tcgcgtcggg atgcaggatg ctatcggctg cctcgcgcac cagccccaaa
     4741 tagctgacgt acagcacggg catgccgtgt tcgttgcggc ggtgggtatc gacaaaggtg
     4801 taaagctgtt ccacatagcc cataggctgc gaagtctgct tggcgaccca cagctttacg
     4861 cccgcctgca gggaattgcg caggggggaa agcgggccgt tgggcaggag cgtgccttgg
     4921 gcgacggtca acacccgcag gccgccgtcg gtaacggcaa tcaatacggg aaccagctcg
     4981 acgatgcttt gcagcggagc ttcggcttcg gggtaggcgt ccatgctcgg ctttctgcgg
     5041 gtgggaaagg ctttattgta aaccaaagcg gtttgtcgga acgaagcggg tcggttttgc
     5101 ggtatcgcaa acctgttttc agacgacctt gcatattata ctcaagttga gcataatgta
     5161 aaaacagttt gaaaaacaga cggcaaacct tcatgccgtc tgaaaaccag ctacaaggaa
     5221 acatcatgca aaccgccgcc cgccgctcgt tcgattacga tatgcccctc atccaaacgc
     5281 cgacttccgc ctgccaaatc cgtcaggcgt gggcgaaggt tgccgacacg cccgaccacg
     5341 agacggcagg tcgtctgaaa gacgaaatca aggccttgct gaagcggaaa aacgcggtct
     5401 tggtggcgca ttattacgtc gatccgctga ttcaggattt ggctttggag acgggcggat
     5461 gcgtgggcga ttcgctggaa atggcgcgct tcggcgcgga acacgaagcc ggtacgctgg
     5521 tagtggcggg cgtgcgtttc atgggcgaga gcgcgaaaat cctctgccct gaaaaaacgg
     5581 tgctgatgcc tgatttggaa gcggaatgtt ctttggattt gggttgtccc gaagaagcgt
     5641 tttcagcgtt ttgcgaccaa caccccgacc gcacggtggc ggtgtacgcc aatacttccg
     5701 cagctgtgaa agcgcgtgcc gactgggtgg taacgtcttc ggtggcgttg gaaatcgtgt
     5761 cgtatctgaa atcgcgcggc gagaaactga tttgggggcc cgaccgccat ctcggcgact
     5821 acatccgccg cgaaacgggc gcggatatgc tgttgtggca gggttcgtgc atcgtccata
     5881 acgaattcaa agggcaggaa ctggcggcgt tgaaggcaga gcatcccgat gctgtggtgc
     5941 tggttcatcc cgaatcgccg caaagcgtca tcgaactggg cgacgtggtc ggctcgacca
     6001 gcaaactact caaagccgcc gtatcgcgcc ctgaaaaaaa attcatcgtg gcaaccgatt
     6061 tgggcatcct gcacgaaatg caaaagcagg cgcccgacaa agaatttatc gccgcgccga
     6121 cggcgggcaa cggcggaagc tgtaaaagct gcgcgttctg cccgtggatg gcgatgaatt
     6181 cgctgggcgg catcaaacac gccctgacag gcggacgcaa cgaaatcctg ttggacagaa
     6241 agctgggcga agccgccaaa ctgcctttgc agcgtatgct cgacttcgcg gcaggactca
     6301 aacgcgggga tgtgttcaac ggcatggggc ccgcctgatt tgccgtccat atcgccgttt
     6361 cagacgacct ctcaaacaag gacaacacca tgcaaaccga ttgcgacgta ttgattgccg
     6421 gaaacgggct ggcggcactg acgctcgccc tgtcgctgcc tgaatcgttc cgcatcgtca
     6481 ttttgtgcaa aaaccggctg gacgacaccg ccagccgtca tgcgcaaggc gggattgcgg
     6541 cggcgtggtc gggagaggac gacatcggaa aacacgtcgc cgatactttg gaagcgggcg
     6601 caggtttgtg cgacgaagcc gccgtccgca ccatcctgtc gcagggcaaa ccggcaatcg
     6661 aatggctgct ggcgcagggc gtggcgttcg accggaatca taacgacctg cacctgacgc
     6721 gcgaaggcgg gcatacctgc cgccgaatcg cccacgtcgc cgactacacg ggcgaagccg
     6781 tcatgcagag cctgattatc caaatacgcc gccgaccgaa catccgcgtt tacgagcggc
     6841 agatggcgtt ggacgttcaa accgaatcag gcgcagcgtg cggactgacc gtcctcgact
     6901 gccgaacgca agaaacctac cgcatccgtg cccgccatac cgtactcgca ggcggcggcc
     6961 tcgggcaaat ttacgccgcc accaccacgc cgcccgaatg cacgggcgac gccatcgcca
     7021 tggcgatacg cgcgggttgc gcaatcgaaa acctcgaatt tatccaattc caccccacag
     7081 gcttggcaag gccgtctgaa aacggccgca ccttcctgat ttccgaagcc gtgcgcggcg
     7141 aaggcggcat cctgaccaat caatcgggcg aacggtttat gccgcattac gaccgccgcg
     7201 ccgaactcgc gccgcgcgac atcgttgccc gcgccatcgc cgccgaaacc gccaaacaaa
     7261 cgcaagactt cgtctcgctc gacatcagcc gccaacccgc agcgttcgtc cgtcggcatt
     7321 tcccgtccat ccatcggcac tgcctgtccc aatgcggctt ggacatcacg cgccaagcca
     7381 tccccgtccg ccccgtgcaa cactacacct gcggcggcat ccaaaccgac cccagcggca
     7441 gaacctccct gccgcagctc tacgccttag gcgaaaccgc ctgtaccgga ctgcacggag
     7501 ccaaccgcct cgccagcaac tccctgctcg aatgcgtcgt taccgccagg cttgccgccc
     7561 aaggcatcgc ggacggacaa gcgttccaaa tcgaaccatt ccaaaggccg tctgaaagcc
     7621 cctccgccga aacagacatc ttttcaaacg acctccaaaa cacattcagc cgccctgtcc
     7681 tgcaaacgtt caaccaacgc catctcggca tcctccgcaa cgataccgac ctgcaccgcg
     7741 ccatcgacca actgcggctt tggaagcaaa accaagccga accgcacacc gcgtccgaat
     7801 acgaaaaccg caacctactc gaatgcagcc tcgccgtcgc ccaagccgca tacaggcgga
     7861 gacagaacat cggtgcgcat tttaatagtg attgttaagg ttggctggtt agaaaaaaat
     7921 cgttcaaata aatttgaata gcatggttaa gagattggca ttcagttttt atggttgcgt
     7981 aagttgggtt gaggcctttg caaaaaagcc cttccttcgg catccgaaac ccaaacacag
     8041 gttttcggct gttttcgccc caaatacctc ctaattttac ccgaatatcc ccttaatcc
//
LOCUS       DAWTQS010000074         7852 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.074, whole genome shotgun sequence.
ACCESSION   DAWTQS010000074 DAWTQS010000000
VERSION     DAWTQS010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7852
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.074"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            276..899
                     /locus_tag="WNA25_001819"
     CDS             276..899
                     /locus_tag="WNA25_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFA9824516.1"
                     /translation="MKKPTDTLPVNLQRRRLLCAAGALLISPLAHAGAQREETLADDV
                     ASVMRSSVGSVNPPRLVFDNPKEGERWLSAMSARLARFVPDEGERRRLLVNIQYESSR
                     AGLDTQIVLGLIEVESAFRQYAISGVGARGLMQVMPFWKNYIGKPAHNLFDIRTNLRY
                     GCTILRHYRNLEKGDIVRALARFNGSLGSNKYPNAVLGAWRNRWQWR"
     gene            1011..2393
                     /locus_tag="WNA25_001820"
     CDS             1011..2393
                     /locus_tag="WNA25_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFA9824517.1"
                     /translation="MARDNRIQMFPHEWRASTTLSGVYALRMLGMFLVLPVLAIYAAS
                     LPGAEDNKTLVGLAMGIYGLTQALLQLPLGIASDKFGRKKTIYVGLVVFAAGSFLAAA
                     ADTLPMLVAARAIQGAGAVSAAVTALLADLTRDGVRTRAMAMIGLSIGLTFSVSLVVA
                     PMIADVAGVRGLFMLTGILTVISIGVVAWMTPDPEVSKLHEDTQAQPSRIGEVLKNRR
                     LLTLDFGIFALHAAQMALFTALPFAMTRLGLEKIQHWKVYLPSTITGLVVMVPLIIVG
                     ETRNKLKQVFVLGIVCIAAAQLGLLSGMHSIWLITAYLVVYFIGFNVLEASLPSMVSK
                     IAPSDLKGTAMGVYNTMQSLGLFAGGAAGGLLFQKYGFAGVFAFCSILMLLWLVIAVL
                     SPAPKPVKNLSYPVGGVWQGNRDGLQRALLQLEGVEDIGFSFDGQTVYLKVLQKGFDQ
                     AAAEKIITGV"
     gene            2397..2921
                     /locus_tag="WNA25_001821"
     CDS             2397..2921
                     /locus_tag="WNA25_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFA9824518.1"
                     /translation="MSLNKVILIGRLGRDPEVRYMPNGEAVCNFSVATSETWNDRNGQ
                     RVERTEWHNITMYRKLAEIAGQYLKKGGLVYLEGRIQSRKYQGKDGIERTAYDIVANE
                     MKMLGGRNENSGGAPYDEGYGQSQEAYQRPAQQSRQPAPDAPSHPQEAPAAPRRQPVP
                     AAAPVEDIDDDIPF"
     gene            3294..4217
                     /locus_tag="WNA25_001822"
     CDS             3294..4217
                     /locus_tag="WNA25_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFA9824519.1"
                     /translation="MENQRPLLGFALALLAAMTWGMLPIAVRQVLKFVDAPTLVWVRF
                     TVAAAVLFVLLALGGRLPKRRDFSWHSFRLLLLGVTGISANFVLIAQGLHYISPTTTQ
                     VLWQISPFTMIVVGVLVFKDRMTAAQKIGLVLLLVGLLMFFNDKFGELSGLGAYAKGV
                     LLCAAGSMAWVCYAVAQKLLSAQFGPQQILLLIYAASAAVFLPFAEPAHIGSLDGTLA
                     WVCFVYCCLNTLIGYGSFGEALKHWEASKVSAVTTLLPVFTVIFSLLGHYVMPDTFAA
                     PDMNGLGYVGALVVVGGAVTAAVGDRPFKRR"
     gene            complement(4282..5670)
                     /gene="fumC"
                     /locus_tag="WNA25_001823"
     CDS             complement(4282..5670)
                     /gene="fumC"
                     /locus_tag="WNA25_001823"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFA9824520.1"
                     /translation="MNTRTEHDTMGNVEVPSEAYWGAQTQRSRNNFKIGGETLPQPLI
                     YALALVKKAAAATNVSLGRIKPEQADLITQAADDVLNGRLDGQFPLVVWQTGSGTQSN
                     MNMNEVLANRANEIAGTGLAAYRPVHPNDHVNRAQSTNDAFPTAIHVAAAIEINRHLI
                     PAVKALRDTLDKKAQAFAPIVKIGRTHLQDATPLTLGQEFSGYVSQLDHSLGRLNDAL
                     KGLYELALGGTAVGTGLNSHPEYAEIAAAKLAELSGLPFVSAPNKFEALGGRDAAVAA
                     SGALKTLAASLNKIANDIRWLASGPRCGLGEIKIPENEPGSSIMPGKVNPTQCEAMTM
                     VCCQVFGNDVTIGMAGASGNFELNVYMPVIAYNLLQSIRLLGDACNSFNENCAAGIEP
                     VPEKIDYFLHHSLMLVTALNRKIGYENAAKVAKTAYKNDKSLRETAVGLGLLTGEEFD
                     ELVVPADMVHPR"
     gene            complement(5814..7793)
                     /gene="tkt"
                     /locus_tag="WNA25_001824"
     CDS             complement(5814..7793)
                     /gene="tkt"
                     /locus_tag="WNA25_001824"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFA9824521.1"
                     /translation="MSQLANVIRFLSADAVQKANSGHPGAPMGMAEMAETLWTKFLNH
                     NPANPKFYNRDRFVLSNGHASMLLYSLLHLTGYNLSIEDLKNFRQLHSKTPGHPEYGY
                     TDGVETTTGPLGQGIANAVGMALAEKILAAEFNKDGLNIVDHYTYVFMGDGCLMEGVS
                     HEACSLAGTLGLGKLIVLYDDNNISIDGKVDGWFTENIPQRFESYGWHVVPNVNGHDT
                     AAIQTAIEAARAETGKPSIICCKTLIGKGSANKEGSHKTHGAPLGADEIEATRKHLGW
                     AYPAFEIPQEIYDAWNAKEKGAKLEAGWNELFAQYQAKYPAEAAEFVRRMDKKLPENF
                     DEYVQTALKEVCAKAETVATRKASQNSIEILAKELPELVGGSADLTPSNLTDWSNSVS
                     VTRDKGGNYIHYGVREFGMGAIMNGLVLHGGVKPFGATFLMFSEYERNALRMAALMKI
                     NPVFVFTHDSIGLGEDGPTHQPIEQTATLRLIPNMDVWRPCDTAESLVAWAEAAKAED
                     HPSCLIFSRQNLKFQARSEQQLNDIKRGAYVISEAQGNAQAVIIATGSEVGLAVEAQK
                     VLAGQGIAVRVVSMPSTSVFDRQDAAYQAAVLPEGLPRIAVEAGHTNGWYKYVGLNGA
                     VVGINRFGESAPADLLFKAFGFTVDNVVDTVKSVL"
BASE COUNT         1524 a         2017 c         2399 g         1912 t
ORIGIN      
        1 ttccgcctgc tgccgcccga aaagcgtaat gcgctgatgg tgctgctgca caatccggac
       61 gacggataac cggtgaaaac gggaaatgcc cgggcggtgt cgtctgtgcc tgtttggaca
      121 agcattcttg agggcggtaa aatcaaggtt tgcccgaaaa gggcagggcg atatgccgcg
      181 cttggatttt gcgcgcggtt tttcgcgttg gaagcaatca tgtctgtttg aatatttaat
      241 gccgtctgaa acgtccggac gcacccccgt tccttatgaa aaaaccgacc gataccctac
      301 ccgtcaatct gcaacgccgc cgcctgctgt gtgccgccgg cgcgctgttg atcagcccgc
      361 tggcgcacgc cggcgcgcaa cgtgaagaaa cgcttgccga cgatgtggct tccgtgatga
      421 ggagttctgt cggcagcgtc aatccgccga ggctggtgtt cgacaatccg aaagagggcg
      481 aacgttggtt gtccgcgatg tcggcacgtt tggcaaggtt cgtccccgac gagggggagc
      541 ggcgcaggct gctggtcaat atccaatacg aaagcagccg ggccggtttg gatacgcaga
      601 ttgtgttggg gctgattgaa gtggaaagcg cgttccggca gtatgcgata agcggggtcg
      661 gcgcgcgcgg tctgatgcag gttatgccgt tttggaaaaa ctacatcggc aaaccggcgc
      721 acaacctgtt cgatatccgt accaacctgc gttacggctg caccatcctg cgccattacc
      781 gaaaccttga aaagggcgac atcgtccgcg cgcttgcccg cttcaacggc agcctcggca
      841 gcaataaata tccgaacgcc gttttgggcg cgtggcgcaa ccgctggcag tggcgttgat
      901 tttgaacccg tgccgcaacc gaaatacggc gaatcctgta taatccgaaa atctgttcac
      961 tggaagttca gacggcattg caactgttga tgccgtctga aaaattattt atggcaagag
     1021 acaaccgcat ccaaatgttt ccgcacgaat ggcgcgccag tacgacgctt tccggcgtgt
     1081 acgcgctgcg tatgctgggt atgttcctcg tgctgcccgt tttggcaata tatgccgcct
     1141 cgctgcccgg tgcggaagac aataaaacgc tggtcgggct ggcaatgggc atttacgggc
     1201 tgacgcaggc attgctgcaa ctgcctttgg gtatcgcttc cgacaagttc gggcgcaaga
     1261 aaaccattta tgtcgggctg gtcgtgtttg ccgccggcag cttccttgcc gccgccgccg
     1321 atacgctgcc catgctggtt gccgcgcgcg ccatacaggg cgcgggggcg gtcagtgctg
     1381 ccgtaaccgc gctgcttgcc gacttgacgc gcgacggcgt gcgtacccgc gcgatggcga
     1441 tgatcggttt gagtatcggt ctgacgtttt cggtcagcct ggtggttgcg ccgatgattg
     1501 ccgatgtggc cggcgttcgc ggactgttta tgctgaccgg cattctgacc gtcatcagca
     1561 tcggcgtagt ggcgtggatg actcctgatc ccgaagtttc caagctgcat gaggacacgc
     1621 aggcgcagcc ttcgcgcata ggcgaagttt tgaaaaaccg taggctgctg acgctcgatt
     1681 tcggcatttt cgccctgcac gccgcgcaaa tggcattgtt taccgcgctg cctttcgcga
     1741 tgacccggct cggtttggaa aaaatccagc attggaaagt atatctgccc tcgaccatta
     1801 cgggcttggt ggtgatggtt ccgctgatta tcgtcggcga aacgcgcaac aagctcaaac
     1861 aggtttttgt tttgggcatc gtctgcatcg cggcggcgca gcttggtttg ctgtccggaa
     1921 tgcactcgat ttggctgatt accgcttatc tggttgttta ctttatcggt tttaatgtgt
     1981 tggaagcgag cctgccgtct atggtttcca aaatcgcgcc gtccgacctg aagggtacgg
     2041 cgatgggcgt gtacaacacg atgcagtcgc tcggactgtt tgccggcggc gcggcaggag
     2101 gcttgctgtt tcaaaaatac ggctttgccg gcgtgtttgc cttttgcagc atattgatgc
     2161 tgctgtggct ggtgattgcc gttttatcgc ccgcgcccaa gcccgtcaaa aacctcagtt
     2221 accctgtcgg cggcgtgtgg cagggcaatc gggacggttt gcaacgcgcc ctgttgcagt
     2281 tggaaggcgt ggaagacatc ggtttcagtt tcgacgggca gaccgtctat cttaaagtgt
     2341 tgcagaaggg tttcgatcag gctgccgctg aaaaaatcat tacaggagtt taaaaaatgt
     2401 cattgaacaa agtcatcctt atcggccgcc tcggacgcga tcccgaagtg cgctatatgc
     2461 ccaacggcga ggcagtgtgt aatttcagcg tcgccaccag cgaaacttgg aacgaccgca
     2521 acggccagcg cgtagagcgc accgaatggc acaacatcac gatgtaccgc aaactggcgg
     2581 aaattgccgg gcaatacctc aaaaaaggcg ggctggttta tctggaaggc agaatccaaa
     2641 gccgcaaata ccaaggcaaa gacggcatcg aacgcaccgc ttacgatatt gtcgccaatg
     2701 aaatgaaaat gttgggcggg cgcaatgaaa acagcggcgg cgcgccttac gatgaaggtt
     2761 acggtcagag tcaggaggct taccaacgcc ccgctcagca aagccggcag cccgctcccg
     2821 acgcgccgtc ccatccccaa gaagcacccg ccgcgccgcg ccgccaaccc gtgccggccg
     2881 ccgccccggt cgaggacatt gacgacgaca tcccgttctg aattttacgg ccggacatcc
     2941 tcgcggaggg aagtcataaa ggacggaaaa accttaaacc ttacggggca aggtttctct
     3001 tttttgcatt tgggccggct tgttgccgcc ccctgcgttg ggcgtaacgt tgcgattgcg
     3061 ccgttcgggc gttttgaggc aagcgatgcc tgccgcactc ccccgctgtt ttcagacggc
     3121 attttaaaat tgaggcggtg ttttcaaata gtgcgaagca atagtctggc ttcagacagt
     3181 ttgtttgaaa tcaaagggtt ggaatcaaac gccaaacgct tgaaccatca ttgcccgatg
     3241 cggcaaaccg gcatcgtccg gcaatacggt tgtttaaaaa tagggaaatc aggatggaaa
     3301 accaaaggcc gctcctaggc ttcgcgttgg cacttttggc ggcgatgacg tgggggatgc
     3361 tgccgattgc cgtgcggcag gtattgaagt ttgtcgatgc gccgacgctg gtgtgggtgc
     3421 gttttacagt ggcggcggcg gtattgtttg ttttgctggc attgggcggg cggctgccga
     3481 agcggcggga tttttcttgg cattcattca ggctgctgct gctcggcgtg acgggcattt
     3541 cggcaaactt tgtgctgatt gcccaagggc tgcattatat ttcgccgacc acgacgcagg
     3601 ttttgtggca gatttcgccg tttacgatga ttgttgtcgg cgtgttggtg tttaaagacc
     3661 ggatgactgc cgcgcagaaa atcggtttgg ttttgctgct tgtcggtttg cttatgtttt
     3721 ttaacgacaa attcggcgag ttgtcgggtt tgggcgcgta tgcgaagggc gtgttgctgt
     3781 gtgcggcagg cagtatggcc tgggtgtgtt atgccgtggc gcaaaagctg ctgtcggcgc
     3841 aattcgggcc gcaacagatt ctgctgttga tttatgcggc aagtgccgcc gtgttcctgc
     3901 cgtttgccga accggcacac atcggaagtt tggacggtac gttggcgtgg gtttgttttg
     3961 tgtattgctg cttgaatacg ttaatcggtt acggctcgtt cggcgaggcg ttgaaacatt
     4021 gggaggcttc caaagtcagc gcggtaacaa ccttgctccc cgtgtttacc gtaatatttt
     4081 ctttgctcgg gcattatgtg atgcctgata cttttgccgc gccggatatg aacggtttgg
     4141 gttatgtcgg cgcactggtc gtggtcgggg gtgcggttac ggcggcggtg ggggacaggc
     4201 cgttcaaacg ccgctagttc gcaggcaacg gaaaatgccg tctgaacgag gcttcagacg
     4261 gcattttatt tgagggaagg attagcgcgg atgaaccata tcggcaggga cgaccagttc
     4321 gtcaaactct tcgcccgtca gcaagcccaa cccaacggca gtttcgcgca gcgatttatc
     4381 gtttttgtag gcggttttgg cgactttggc ggcgttttcg tagccgattt tgcggtttaa
     4441 cgcagtaacc agcatcaggg aatggtgcag gaaatagtcg attttttccg gtacgggttc
     4501 gatgccggcg gcgcagtttt cgttgaagct gttgcacgcg tcgcccaaga ggcggatgga
     4561 ttgcaagagg ttgtaagcga taacgggcat atagacgttc agctcgaaat tgcccgatgc
     4621 gcccgccata ccgatggtaa cgtcgttgcc gaacacttgg cagcacacca tcgtcatcgc
     4681 ttcgcattgg gtcgggttga ctttgcccgg cataatggac gaacccggtt cgttttcggg
     4741 gattttgatt tcgcccaagc cgcagcgcgg gccgcttgcc agccagcgga tgtcgttggc
     4801 gattttgttc aggcttgccg ccagcgtttt caatgcgccc gaagcggcaa cggcggcatc
     4861 gcgtccgccc aaggcttcaa atttgttcgg cgcgctgaca aacggcaggc cggacagttc
     4921 ggcgagtttg gcggcggcta tttcagcgta ttcgggatgg ctgttcaaac ccgtaccgac
     4981 cgccgttcca cccaaggcga gttcgtacaa acctttgagc gcgtcgttca gacggcctaa
     5041 gctgtggtcg agttgggaaa cgtagccgga aaattcttgt cccaaagtca gcggcgtcgc
     5101 atcctgcaag tgggtacggc cgattttgac gatgggggcg aaggcttggg cttttttgtc
     5161 caatgtatcg cgcaaggctt ttaccgccgg aataaggtgg cggttgattt caatcgcggc
     5221 ggcgacgtgg atggcggtcg ggaacgcgtc gttggtcgat tgcgcgcggt tcacatggtc
     5281 gttggggtgg acgggccggt acgccgccaa acccgtacct gcaatttcgt tggcgcggtt
     5341 tgccagcact tcgttcatgt tcatattgga ctgcgtaccg gaaccggtct gccaaaccac
     5401 taaggggaac tgcccgtcga gcctgccgtt cagtacatca tccgccgcct gcgtaatcaa
     5461 atccgcctgt tcgggcttaa tcctgccgag ggaaacattg gtcgcggcgg cggctttttt
     5521 caccaacgcc aaagcgtaaa tcagcggctg cggcagggtt tcgccgccga ttttgaaatt
     5581 gttgcggctg cgctgggtct gcgcgcccca ataggcttcg gacgggactt cgacattgcc
     5641 catcgtgtcg tgttcggtgc gggtgttcat acgtttctcc ttttgaaatg tgaataagag
     5701 tgattcgcaa atattataat ggagattggc ggaatgagga agcatcaagg cgggagggca
     5761 aaaaaatgcc gtctgaaaag cattcagacg gcatttgatg cggcggctga ggtttacagc
     5821 acggatttca ccgtatcaac cacattgtcc acggtaaagc cgaatgcttt gaacagcaga
     5881 tcggcagggg cggattcgcc gaagcggttg atgccgacga ctgcgccgtt cagtccgaca
     5941 tatttgtacc agccgttggt gtgtccggct tctacggcga tgcgcggcag gccttccggt
     6001 aggacggcgg cttgataggc ggcgtcttgg cggtcgaaga cgctggtgga cggcatggaa
     6061 acgacgcgca cggcaatacc ttgtcctgcc aatacttttt gcgcttctac agccaatcct
     6121 acctcggaac cggtggcaat gatgacggct tgggcgttgc cttgggcttc actgattacg
     6181 taagcaccgc gtttgatgtc gttcagttgt tgctcgctgc gtgcttggaa tttcaggttt
     6241 tgacggctga aaatcaggca ggacgggtgg tcttcggctt ttgccgcttc tgcccaagcg
     6301 accagagatt ctgcggtgtc gcacggccgc catacgtcca tattcggaat caggcgcagg
     6361 gtggcggttt gctcgatagg ctggtgggtt gggccgtcct cgcccaaacc gatggaatcg
     6421 tgggtgaata cgaagacggg gttgattttc atcagtgccg ccatgcgtaa ggcgttgcgt
     6481 tcgtattcgc tgaacatcag gaaagtcgcg ccgaagggtt ttacaccgcc gtgcaatacc
     6541 aaaccgttca taatcgcacc cataccgaac tcgcgcacgc cgtagtggat gtagttgccg
     6601 cctttgtcgc gggtaacgga gacgctgttt gaccagtcgg tcaggttgga cggggtcagg
     6661 tcggcagaac cgcccaccag ttcgggcagc tcttttgcca agatttcgat gctgttttgg
     6721 ctggctttgc gggtggcgac ggtttccgct ttggcgcaca cttctttcaa tgccgtctga
     6781 acgtactcgt caaagttttc cggcagcttt ttatccatgc ggcgcacgaa ttctgcggct
     6841 tcggcaggat atttggcttg atattgcgcg aacagttcgt tccatccggc ttccagtttc
     6901 gcgccttttt ctttggcatt ccacgcatcg taaatttctt gcgggatttc aaacgcagga
     6961 taagcccaac ccaaatgttt gcgcgtggct tcgatttcgt ccgcacccaa aggcgcgccg
     7021 tgggttttgt ggctgccttc tttgttggcg ctgcctttgc cgattaaggt tttgcagcag
     7081 atgatggacg gcttgccggt ttcggcgcgg gcggcttcga tggccgtctg aatggcggcg
     7141 gtgtcgtggc cgtttacgtt gggaacgacg tgccagccgt agctttcaaa gcgttgcggg
     7201 atgttttcgg taaaccagcc gtccactttg ccgtcgatgg agatattgtt atcgtcatac
     7261 aaaacaatca gtttgcccaa gcccaaggtg ccggcgagtg agcaggcttc gtgcgatacg
     7321 ccttccatca ggcagccgtc gcccataaag acgtaagtgt agtgatcgac gatgttcaaa
     7381 ccgtctttat taaattcggc ggcaaggatt ttttctgcca atgccatgcc cactgcgttg
     7441 gcaatacctt gccccaacgg gccggtcgtg gtttccacgc cgtcggtgta gccgtattcg
     7501 ggatggccgg gggttttgct gtgcagctgg cggaagtttt tcagatcttc aatgctcagg
     7561 ttgtagccgg tcaggtgcag caggctgtac aacagcatag acgcatgacc gttggagagg
     7621 acgaagcggt cgcggttgta gaatttggga ttggcgggat tgtgattgag gaatttcgtc
     7681 cacaatgttt ccgccatttc cgccataccc ataggcgcgc cggggtggcc ggaattggct
     7741 ttttgaacgg catcggccga gaggaagcgg attacgtttg ccagttgaga cattttgtat
     7801 tttccttgct ggtgtttcgg ataagtggat aatcggaagg cgttgattat cg
//
LOCUS       DAWTQS010000075         7733 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.075, whole genome shotgun sequence.
ACCESSION   DAWTQS010000075 DAWTQS010000000
VERSION     DAWTQS010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7733
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.075"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(20..1786)
                     /locus_tag="WNA25_001825"
     CDS             complement(20..1786)
                     /locus_tag="WNA25_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFA9824522.1"
                     /translation="MIERLKAVENQAEAMGRGARSAYLKQQAQELGISLATLYRKLEA
                     VSVKPTRKRRSDAGKTELKPEEAKLISAVLAEAMRRNGKRLMPVRQAVEMLRANGKIE
                     AARIDGETGEVIPLSENTITRALREYKLHSDQLLQPDPVSRMKSEHPNHCWQIDPSLC
                     VLYFLPRQGKDTGLRVMKEEEFYKNKPKNVVKIENDRVWRYTGTDHASGTISVRYYFG
                     GETSANLCDFFIYMMQAKKDIGKDPFRTVPRMVMLDPGSANTSAAFKNLCKPLDVHVQ
                     INKPGNPRAKGQVEKANDIVETAFESGLRFTEVHDIDQLNALSERWMRYYNGTQKHSR
                     HGMTRYQAWNKIKPEQLILPPPAEYCRELAVSAPKEAKVSADLEIRFGGRVYSVKGIK
                     GILVGKKVLVGKNPWEANGARVATYDAEGNEVWVSVPEVVFDEMGFKADAAVIGAEYK
                     APADTDAQRHRKELDKLAMGAETLEAAAAKRKGKAVPFGGKIDPYKHQEDTLAASNTL
                     FMPKQGQQMDYNKMEVAEQVLSKVEIAKRLKPRVEADGGDWKQAVSVILKHYPEGVTE
                     GRLEEAFERTRTRCRLKLLKTG"
     gene            complement(1779..2652)
                     /locus_tag="WNA25_001826"
                     /pseudo
     CDS             complement(1779..2652)
                     /locus_tag="WNA25_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009426280.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2689..2973)
                     /locus_tag="WNA25_001827"
     CDS             complement(2689..2973)
                     /locus_tag="WNA25_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFA9824523.1"
                     /translation="MATSKKAQRLLRVFLAMDAHPVIGISNKELSDGLGLTPSQVSRD
                     IDDLVASGLVIKLENGNYAYGIKTLQIAERFRKQQERLNARLQELENRIY"
     gene            complement(2960..3190)
                     /locus_tag="WNA25_001828"
     CDS             complement(2960..3190)
                     /locus_tag="WNA25_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFA9824524.1"
                     /translation="MEKPLNFKPIPYPQTRGSTAVWFKRNGVCKTHRAKHFNLERTAV
                     EHPLRGKLKGNFGKSHEAAVKFGLKEDADGDE"
     gene            3462..3842
                     /locus_tag="WNA25_001829"
     CDS             3462..3842
                     /locus_tag="WNA25_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFA9824525.1"
                     /translation="MEKTSLFGKRLKEERIKLGLNQAEAAEKCGFSREMWGKWERGEN
                     RPSSEKLFSFSKIGIDIDYVMHGRRGETAAMPSESLSAEEKELLALFRQLGSGSRKEL
                     ADYAAFKLVVEKKAQTALGKVSNG"
     gene            4076..5116
                     /locus_tag="WNA25_001830"
     CDS             4076..5116
                     /locus_tag="WNA25_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFA9824526.1"
                     /translation="MKKIITHRIYPKNENRERVEPKISTHLIDLPITARRTLETRLTK
                     ALGNKSHGIEMSVVNTAENSFFQIAAAIQLKEEAEFIEDSAQFAHMLTDAQLNTNAPG
                     GILLVLKGRVGDTGKPFLCVIKAEPQDGFRTKEEDDFITIEFLEELLLTDSARLFKIG
                     FLVAETVRPLEQIQSGNYRAFLYDHLMTQTETRPAASYFYQVFLGMSIAASSRKLTQD
                     FFEWTRNFIDNSDLSDDAKLDAHEALRVTLKSAEATISVNNFAQNHLPQEKRTTYTEF
                     MVEKDFPQNAVSKDIEYIKTRLRKRRSYGFSNGVVILTPPEHTQDYMEIAPTEDGEYT
                     VVLIKGQLQQQK"
     gene            5113..5487
                     /locus_tag="WNA25_001831"
     CDS             5113..5487
                     /locus_tag="WNA25_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824527.1"
                     /translation="MSDIDDFKSYLESHQAAFSAWGRFVAEEIQNQLSNVISPVPVAN
                     FLKIEAKPGVKEISSALAKIGRKNYTSPQTQMTDLVGVRFVALLAEHIQIVCEIIESS
                     SQWNAKVSKDFADEIQQNPKAI"
     gene            5484..6122
                     /locus_tag="WNA25_001832"
     CDS             5484..6122
                     /locus_tag="WNA25_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824528.1"
                     /translation="MTIQSKHYEIRPKQAFITPENVSIPADLCCEVQVRSLLQHAYAE
                     LVHDNIYKPDGNVPKQAEREVAKSMALMETTDDLFSRTLAILKEANQPQEELLPQLSQ
                     LYQKEIGLVPEVDKKTNMIFLETFQSSISQSSILSDIRSLLNEKKYIAKRIKENAEEM
                     YFFSQPAALLVYWLIEKVGADEVWKKWPLPAYNKNLKFICTDLDKQPSHELF"
     gene            6242..6529
                     /locus_tag="WNA25_001833"
     CDS             6242..6529
                     /locus_tag="WNA25_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824529.1"
                     /translation="MIEFVRAKKRLLWAFVLLLAWACGYRYAADKAEAEQTALIAAYR
                     HSSMVAAEQYALQLKKAQDERQRWYDFSQKQSTDLAAALSELDKTRNTLQE"
     gene            6603..6824
                     /locus_tag="WNA25_001834"
     CDS             6603..6824
                     /locus_tag="WNA25_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824530.1"
                     /translation="MPSDTPIKTVAVAEIPPVPSGLLVEYERPERPAGGSPEQLLNHA
                     VRYGGYYRKLEIQIEGWQNWHTKGRLKHD"
     gene            6817..7026
                     /locus_tag="WNA25_001835"
     CDS             6817..7026
                     /locus_tag="WNA25_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFA9824531.1"
                     /translation="MTDFADRASEREAEFLVEALAKHQPPSENTAGFSHCEDCGDPIP
                     EAKRKAVRGCTRCVVCHEYSQLKTK"
     gene            7294..7614
                     /locus_tag="WNA25_001836"
     CDS             7294..7614
                     /locus_tag="WNA25_001836"
                     /inference="COORDINATES: protein motif:HMM:NF013842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824532.1"
                     /translation="MSRNTLYLRIRLKKQTGSLKHQVTGLNAAKSDRQKPARYVGRHP
                     DACLHEIAKHFDCTAAAVCHAPKQMRMARKKDRRLQRTRPGQSNALFDTAGRIFRLPT
                     CLSG"
BASE COUNT         1938 a         1972 c         1815 g         2008 t
ORIGIN      
        1 agctgttttc ggttgaaacc taaccggttt tcaaaagttt cagacggcat cttgtccggg
       61 tgcgttcaaa cgcctcttcc agcctgccct cggtcacacc ttccgggtag tgtttgagga
      121 tgaccgacac tgcctgtttc cagtcgccgc cgtctgcctc gacgcggggt tttaagcgtt
      181 tggcgatttc gaccttgctc aatacctgct cggcgacctc catcttgttg taatccatct
      241 gttgcccctg tttgggcata aagagcgtat tgcttgcggc aagcgtatct tcttgatgtt
      301 tgtacgggtc gattttgccg ccgaacggga ctgccttgcc tttgcgtttg gcggctgccg
      361 cctccagcgt ttccgcaccc atcgccagct tgtccagctc tttgcgatgc cgttgcgcgt
      421 ctgtatcggc cggggctttg tattccgccc cgatgaccgc ggcatcagcc ttgaagccca
      481 tctcgtcaaa aactacttcg ggtacggata cccaaacctc gttaccctcc gcgtcataag
      541 tggcgacccg cgccccgttt gcctcccaag gattcttacc gaccaaaact ttcttaccga
      601 ccaaaatccc cttgatgcct tttacgctat atacccgtcc gccaaagcgg atttccaaat
      661 ccgccgagac tttcgcctct ttcggcgcgc tgacggcaag ctctcggcaa tattcggcag
      721 gcggcggcag gatgagctgc tcgggtttga ttttgttcca cgcctgatag cgggtcatgc
      781 cgtggcggct gtgtttttgc gtaccgttgt agtaacgcat ccagcgttcc gataaagcat
      841 tgagctggtc gatgtcgtgt acctcggtaa agcgcaaccc gctctcaaat gccgtttcca
      901 caatatcgtt ggctttttcc acttgccctt tggcacgcgg attgcccggc ttgttgattt
      961 gcacatgcac atccaagggc ttgcacaaat ttttaaacgc cgccgaagta ttcgcgctgc
     1021 ccgggtcaag catgaccatg cgcggtacgg tccggaacgg gtctttgccg atgtcttttt
     1081 tcgcctgcat catgtagatg aaaaaatcac agaggttcgc gctggtttcg ccgccgaagt
     1141 aataacgcac cgaaatcgtg ccggaggcat ggtctgtccc cgtgtaccgc caaacgcggt
     1201 cgttttcgat tttgacgacg tttttcggct tgtttttata aaactcctct tccttcatca
     1261 cccgcagccc cgtatccttg ccctgacgcg gcaggaaata caaaacgcac aaactcgggt
     1321 cgatttgcca acaatggttc gggtgttccg atttcatgcg gctgacgggg tcgggctgca
     1381 aaagctggtc ggaatgcagc ttgtattccc gtaaagcccg ggtaatggtg ttttcagaaa
     1441 gggggatgac ttccccggtt tccccgtcaa tccgcgccgc ctcgattttc ccgttggcgc
     1501 gcagcatttc caccgcctgc cgcaccggca tcaaccgctt gccgttgcgc ctcatcgcct
     1561 ccgccaaaac cgccgaaatc aatttggctt cttccggttt aagctccgtc ttgcccgcat
     1621 cgctgcgccg tttgcgcgtc ggcttgacgc tgaccgcctc cagcttgcgg tatagcgtgg
     1681 caaggctgat gcccaattcc tgcgcctgct gcttaagata tgcagagcgt gcgccgcgcc
     1741 ccattgcttc cgcctgattt tcgactgctt taagacgctc aatcattgcc ggattcatcg
     1801 ccttctcccg tttcaccgcc caaccattcc ggcacattgt ctgccggtgc ttcggtcggt
     1861 agggcatagc tttcgcgcag ttgctcgcag tccaaaataa tttgattgag cgtgccgacc
     1921 atcttcgcgc ggtggtcaaa tccatgcgcc tcgccgtgcg ccgccatctg ctcgaacata
     1981 tcgcgcaatc ggctgatttg cgagcggata ccgacttcca agctgccaag ctgcatcgtc
     2041 agctcgatgc ccacatccgc aggtttaggc tctttgacac ccgtctgctt tttcgacagc
     2101 ttttccgcca gctcatcgac ctttttattt ttatcggcaa tcaccttatc tttcgcttcc
     2161 gccgtttcac ggctttcgcg cagggcgacg cgcagctcct taatagtcat acggtcgaca
     2221 tcgtcaaaag tgttgccgtt gacctcaccg ccttcggcaa gctccaacaa agtgtcgtcg
     2281 tcttcgacca gcagctcgag cagtttggat ttgcccaacg tcatcagctt aggctgcgct
     2341 tgtttcattt tcgggtcgat aaaacggagg gtggcattca tcagtctttg ggattcccgc
     2401 cgtccgaggc caactctttt tcagcgattt cggcaaaccg cccatgcggc gtatgctctt
     2461 tgatgatgat cagcgcgcgt cccggctcaa acatcccttc catcgtttgg cgtccgcaaa
     2521 tcgtccgcgc tcaatccata ccgtctcgtt gtaggcctcg ccgcctgaaa acttgtccat
     2581 aacctccata ctgtggatag ccaattcgtt tgccgttacg ccgaccgtat gtcctaaaat
     2641 ttctgttgcc atttcttttg ctcctcaaat gcgacgtcgt cgtcgcattt agtaaattcg
     2701 gttttccaat tcttgcaagc gcgcattcag ccgctcttgc tgttttctga accgctctgc
     2761 gatttgtagg gttttgatgc cgtaggcgta gttgccgttt tcaagcttga tgaccaatcc
     2821 cgatgcaacc aaatcatcaa tatccctgct gacttgtgat ggcgtcagcc ccaggccgtc
     2881 tgaaagctcc ttattgctga tgccgattac cggatgcgcg tccatcgcca aaaacacccg
     2941 caacagccgc tgcgcctttt tactcgtcgc catccgcatc ctccttcagc ccaaatttta
     3001 ccgccgcttc gtggcttttg ccgaaattgc ctttcagctt gccgcgcaag gggtgttcca
     3061 ccgcggtgcg ctccaaattg aaatgtttcg cccggtgcgt cttgcacacg ccgttgcgct
     3121 taaaccacac ggccgtgctg ccgcgcgttt gcggataggg aataggcttg aaatttaaag
     3181 gtttttccat attatttatg cctttcgtgt ggtataatgt gtttagtttt aggcctcttt
     3241 gcacgataaa catccggtct gttgtgcagc agcagggtct tggcttgttt taaaataaac
     3301 aagctttttc ccgtagccgg gtgtatgtga tccatcatga gctgtcgcgc atgagccttc
     3361 aaagtgttca ttttttcgtc ctttttcgtg atgatttagg gtgtttcatg tcttgatgtg
     3421 tgaattatga gaaaaatatt tctcttttca aggaaattta agtggaaaaa acttctcttt
     3481 ttggtaagcg attgaaagaa gaaagaatta aattaggctt gaaccaagca gaagctgctg
     3541 aaaaatgtgg tttttctcgt gagatgtggg gaaagtggga gcgtggtgaa aatcgcccat
     3601 caagtgaaaa attattctct ttttctaaaa tcggcatcga tattgattac gtcatgcacg
     3661 gcagacgcgg cgaaacagcc gccatgccgt ctgaatcctt gagtgctgaa gaaaaagaac
     3721 tgcttgccct gttccgtcag ctcggcagcg gcagccgcaa agagcttgct gactacgccg
     3781 cctttaagct ggtggtagag aaaaaagccc aaactgcgct tggtaaagtg agcaacggat
     3841 aaaaaatgcc gtctgaagat ttcagacggc atttgggaca ctctgacagt cgggctgctc
     3901 cggtgttctg aaacagatat taagcaatag gagcggccgt gcattttaaa gcgtattaaa
     3961 aaccaccccg atattcaggt catcggccaa gtggttcaga tttcaaaaga ccttaattaa
     4021 cttacaatag gaaaaatgat gacagaaacg aattactctt ttgaagggct ggtaattgaa
     4081 aaaaattatt acccatcgta tttatccgaa aaatgaaaat agagagcgtg tagaacctaa
     4141 aatcagtaca catttgattg atttgcctat tacggcaaga agaacattgg aaaccaggct
     4201 gaccaaagct ttgggtaata aatctcatgg cattgaaatg tctgttgtca atacggctga
     4261 aaatagtttt tttcagatag cggcagccat acaactcaaa gaggaagcag agtttattga
     4321 ggattccgca caatttgccc acatgttgac cgatgcacaa ctgaatacaa atgcaccggg
     4381 tggtattttg ttggttttaa aaggtagggt tggagatacc ggtaagccgt ttttatgtgt
     4441 aattaaggct gaacctcaag atgggttccg aaccaaagaa gaggatgact ttatcacgat
     4501 tgaattctta gaagaattat tactgaccga ttcagcaaga ttattcaaga taggtttttt
     4561 ggtggctgaa acagtaaggc cgctagagca aatacaatct gggaattatc gagctttttt
     4621 gtatgaccat ctgatgacac aaacggaaac tagaccggca gcttcctatt tctatcaagt
     4681 attcttgggt atgagtatag ctgcttcttc ccgtaaattg acgcaggatt tttttgagtg
     4741 gacacgcaat tttatcgata actctgattt aagtgatgat gcaaaattag atgcgcatga
     4801 agcattgcgc gttacattga aaagtgcgga agcaaccatt agtgtaaata attttgccca
     4861 aaatcattta cctcaagaaa aacgaacaac ttatacagaa tttatggtgg aaaaggattt
     4921 tcctcaaaat gccgtaagta aagatattga atatattaaa actcgtttac gcaaacggag
     4981 gtcttacgga tttagtaacg gtgtagttat cttgactcct cccgagcata ctcaggacta
     5041 tatggaaatt gcgccaacgg aagatgggga atatactgtt gtcctaatta aaggacagtt
     5101 acaacaacaa aaatgagtga tattgatgat ttcaaaagct atttagaaag ccaccaagcg
     5161 gcattttccg cttggggtag atttgtggct gaagaaattc aaaatcaatt atccaatgtc
     5221 atttctcctg ttccggttgc caattttcta aaaattgaag caaagcctgg agtcaaagag
     5281 atttcttcag ctctagccaa gattggccga aaaaattaca cttcacctca aactcaaatg
     5341 actgacttgg tcggcgtacg ttttgtggca ttgcttgcgg aacatattca aatagtttgt
     5401 gaaatcattg aatcctcatc tcagtggaat gctaaagttt cgaaagattt tgcagatgaa
     5461 attcagcaaa atcccaaagc aatttgacta tacaatccaa acattacgag attcgtccta
     5521 aacaagcatt cattacaccc gaaaatgtca gcatccctgc tgatttgtgc tgtgaagttc
     5581 aggtgcgttc tttactccaa catgcttatg cagagctggt tcatgacaat atatacaagc
     5641 ctgacggcaa tgttccaaag caagcagagc gcgaagtcgc gaagagcatg gctttgatgg
     5701 aaactacaga tgacttattt agtcgtactc tggcaatttt gaaagaagca aatcaaccac
     5761 aagaagagct tcttccccaa ttatcacagc tttatcaaaa ggaaataggg cttgttcctg
     5821 aagttgataa aaaaaccaat atgatttttc tagaaacatt tcaatccagc atttcccaat
     5881 caagtattct ttctgacatc cgttcacttt tgaatgagaa gaaatatatt gccaagcgga
     5941 taaaagaaaa tgctgaagaa atgtattttt tcagtcagcc agcagctttg ttggtctatt
     6001 ggctgattga gaaagttggt gcagatgaag tatggaaaaa atggcctttg cctgcataca
     6061 acaaaaacct gaaattcatt tgtactgatt tggataagca accatcccat gaactctttt
     6121 aacccccttt aaaagcccat tcagacggcc tttcctaatt atgccttaat caaacgcggc
     6181 atagcggcag ttccgcagct ggcggaaatc aaaaaatccg caaatcagga agggggcggc
     6241 aatgattgaa tttgtccgag ccaaaaaacg gctgctttgg gcatttgtac ttttgcttgc
     6301 gtgggcgtgc ggctaccggt acgctgccga caaggccgaa gcggaacaaa ccgccctgat
     6361 tgccgcctat cggcattctt ctatggttgc ggcggaacaa tacgccttgc agcttaaaaa
     6421 agcgcaggac gaaaggcagc ggtggtacga cttttcccaa aaacaaagca cggatttggc
     6481 ggccgccctg agcgaattgg acaaaacgcg caacacctta caggagtaaa cccgtgaagc
     6541 ggcagacaaa gacggcaacc gttttaacgg ccttggcacg aacagcctgc acctctacaa
     6601 ccgtgccttc ggataccccg attaaaaccg ttgccgtcgc ggaaattccg cccgttcctt
     6661 ccggactgct ggtcgaatac gaacgccccg agcgtccggc cggcggctct cccgaacaac
     6721 ttttaaacca cgccgtccgc tatggcggat actaccgaaa gctcgaaatc caaattgagg
     6781 gctggcagaa ctggcacacg aaaggccgtc tgaaacatga ctgattttgc cgaccgcgca
     6841 tccgaacgcg aagccgaatt tctggtggaa gccttggcga aacaccaacc gccgtctgaa
     6901 aacaccgccg gcttcagcca ttgcgaagac tgcggcgacc cgataccgga agcaaagcga
     6961 aaagccgtcc gaggctgtac gcgctgtgtt gtctgccacg aatatagtca attaaaaaca
     7021 aaatagtaca acactcgacg ttgaaggtct aaccatggca tactctgcgg acttaagaaa
     7081 caaagcctta aaccatagcg gattaacaaa aatcaggaca aggcggcggg ccgcaggcag
     7141 tacgaatggt acggaaccga tccgcccggc gcttcattac cttagggaat cgtcctcttc
     7201 gagccggggc ggggcaacgc cgtaccggtt tttgttaatc cgctatatta cggacaatgc
     7261 aaaaacaccg gccaaaccgc agcaacgttt aacttgtcaa gaaacacgct ttacctgcgg
     7321 attcgcctta aaaaacaaac aggcagccta aaacatcaag ttaccggcct aaatgccgcc
     7381 aaatcggata ggcaaaaacc ggctcgatat gtcgggcggc atccggatgc ctgtctgcat
     7441 gaaatcgcca aacattttga ttgtacggca gccgccgttt gccatgcgcc caaacagatg
     7501 cggatggcgc gcaaaaaaga ccgccgctta caaaggacaa gacccggcca aagtaacgca
     7561 ttatttgaca cggccggccg gattttccga ctgccaacgt gtttgtccgg atgaaacagg
     7621 attcgaccgc cgcctgttcc gcccctatgc ccgcagcctg aaagggcaaa tggcgaaagc
     7681 gcggataagt gggaaaagat accggcgctt atctttggtg tccgcacaag tcg
//
LOCUS       DAWTQS010000076         7698 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.076, whole genome shotgun sequence.
ACCESSION   DAWTQS010000076 DAWTQS010000000
VERSION     DAWTQS010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7698
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.076"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(38..763)
                     /locus_tag="WNA25_001837"
     CDS             complement(38..763)
                     /locus_tag="WNA25_001837"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFA9824533.1"
                     /translation="MRIPLLAPDNYAFPDPAYALARCDGLVGVSRDLDAGRLLEAYRN
                     GVFPWFSRDGWFFWYAVGPRAVIVPERLHVPRSLAKTLRNGSYRVAVNGCFAEVVAHC
                     AAAARPNQDGTWIAPEFQTAYLKLHEMGHAHSFECHYPDENGKTRLAGGFYGVQIGRV
                     FYGESMFALQPDASKIAFACAVPFLADLGVELIDCQQDTEHMRRFGSELLPFADFAER
                     LRMLNAVPLKEEIGRREVACKGL"
     gene            complement(833..1336)
                     /gene="fur"
                     /locus_tag="WNA25_001838"
     CDS             complement(833..1336)
                     /gene="fur"
                     /locus_tag="WNA25_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFA9824534.1"
                     /translation="MIVGKQAEIAYYSVYYFTGILNIMEKFSNIAQLKDSGLKVTGPR
                     LKILDLFEKHAEEHLSAEDVYRILLEEGVEIGVATIYRVLTQFEQAGILQRHHFETGK
                     AVYELDKGDHHDHIVCVKCGEVTEFHNPEIEALQDKIAEENGYRIVDHALYMYGVCSD
                     CQAKGKR"
     gene            1494..1871
                     /locus_tag="WNA25_001839"
     CDS             1494..1871
                     /locus_tag="WNA25_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFA9824535.1"
                     /translation="MNKTLILALSALFSLTACSVERVSLFPSYKLKIIQGNELEPRAV
                     AALRPGMTKDQVLLLLGSPILRDAFHTDRWDYTFNTSRNGIIKERSNLTVYFENGVLV
                     RTEGDALQNAAEALRAKQNADKQ"
     gene            1881..2690
                     /gene="dapB"
                     /locus_tag="WNA25_001840"
     CDS             1881..2690
                     /gene="dapB"
                     /locus_tag="WNA25_001840"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFA9824536.1"
                     /translation="MIPLKIAIAGANGRMGRVLVEAVNNHPDTVLSGALEHSGSEALG
                     LDAGYAVGLKTGIAISDDVDAVLAQSDVLIDFTRPEPTLKHLQKCVEKQVNIIIGTTG
                     FDDAGKAAIRAAAEKTGIVFAANFSVGVNLTFHILDTVARVLNEGYDIEIIEGHHRHK
                     VDAPSGTALRMGEVIAGALGRDLKQCAVYGREGHTGPRDPSTIGFATVRAGDIVGDHT
                     ALFATDGERVEITHKAGSRMTFAAGAVRAAVWVNGKTGLYDMQDVLGLNNR"
     gene            complement(2804..3118)
                     /locus_tag="WNA25_001841"
     CDS             complement(2804..3118)
                     /locus_tag="WNA25_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824537.1"
                     /translation="MFHLFFVNFYFRQLCPTPNYTDWVKIKFKQFSYLKFIYGYATEN
                     QDKDIDNTLELGELKQDDEILDYGGALALIRRRYNLPTSFIIDIVCREIELEFLDQES
                     FN"
     gene            complement(3164..3322)
                     /locus_tag="WNA25_001842"
     CDS             complement(3164..3322)
                     /locus_tag="WNA25_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824538.1"
                     /translation="MDYFPEAARITKKGGEIVINGTSNNKYLRGIPNETELARMGLRL
                     KYNGSLTD"
     gene            complement(3330..4502)
                     /gene="lpxB"
                     /locus_tag="WNA25_001843"
     CDS             complement(3330..4502)
                     /gene="lpxB"
                     /locus_tag="WNA25_001843"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFA9824539.1"
                     /translation="MWGQTNMVDKKSPLIAVSVGEASGDLLGAHLIRAIRKRCPQARL
                     TGIGGELMKAEGFESLYDQERLAVRGFVEVVRRLPEILRIRRELVRDLLSLKPDVFVG
                     IDAPDFNLGVAEKLKRAGIPTLHYVSPSVWAWRRERVGKIVHQVNRVLCLFPMEPQLY
                     LDAGGRAEFVGHPMAQLMPLEDDRETARKTLGADVGIPVFALLPGSRVSEIDYMAPVF
                     FQTALLLLERYPAARFLLPAATEATKRRLAEVLQRPEFAGLALTVTDRQSETVCRAAD
                     AVLVTSGTATLEVALCKRPMVISYKISPLTYAYVKRKIKVPHVGLPNILLGKEAVPEL
                     LQSEAKPEKLAAALADWYEHPDKVAALQQDFGALHLLLKKDTADLAARAVLEEAGC"
     gene            complement(4498..4598)
                     /locus_tag="WNA25_001844"
                     /pseudo
     CDS             complement(4498..4598)
                     /locus_tag="WNA25_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(4653..5645)
                     /locus_tag="WNA25_001845"
     CDS             complement(4653..5645)
                     /locus_tag="WNA25_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFA9824540.1"
                     /translation="MKTHEISKGSVSLIGVAEHEAGQRLDNYLIKILKGVPKGYIHRI
                     IRAGEVRLNKKRCKPDSRIAEGDTVRIPPVRVAEKEMPSERRAAVPARAFEVVYEDDA
                     LLVVNKPSGVAVHGGSGVSFGVIEQLRRARPEAKYLELVHRLDKDTSGLLMVAKKRSA
                     LVKLHEAIRNDHPKKIYLALGVGKLPDDNFHVKLPLFKYTGAQGEKMVRVSEDGQSAH
                     TVFRVLSRFSDGILHGVGLSHLTLVRATLKTGRTHQIRVHLQSQGCPIAGDERYGDYQ
                     ANRRLQKLGLKRMFLHASELHLNHPLTGEPLVLKAEPPPDLAQFAVMLENGTKM"
     gene            6138..>7698
                     /locus_tag="WNA25_001846"
     CDS             6138..>7698
                     /locus_tag="WNA25_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HFA9824541.1"
                     /translation="MKRMLFNATQAEELRVAIVDGQNLLDLDIETLGKEQRKGNIYKG
                     IITRIEPSLEACFVDYGTDRHGFLPFKEVSRSYFLGYEGGRARIQDVLKEGMEVIVQV
                     EKDERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRIEGEERQELKAAMAQLDIPN
                     GMSIIARTAGIGRSAEELEWDLNYLKQLWQAIEEAGKAHHDPYLLFMESSLLIRAIRD
                     YFRPDIGEILVDNQEVYDQVAEFMSYVMPGNAGRLKLYEDHTPLFSRFQIEHQIESAF
                     SRSVSLPSGGAIVIDHTEALVSIDVNSARATRGADIEDTAFKTNMEAAEEVARQMRLR
                     DLGGLVVIDFIDMENPKHQRDVENVLRDALKKDRARVQMGKLSRFGLLELSRQRLKPA
                     LGESSHAACPRCAGTGVIRGIESTALHVLRMVQEEAMKDNTGEVRAQVPVDVATFLLN
                     EKRAELFAMEERLDVNVVLIPNIHLENPHYEINRIRTDDVEEDGEPSYKRVAEPEEDE
                     SAKPFGGEKAKA"
BASE COUNT         1864 a         2462 c         1813 g         1559 t
ORIGIN      
        1 ccactatatt tgaacctgac cgaagcataa gccgccatca aagccccttg cacgccactt
       61 cgcgccgccc gatttcctct ttcaacggca cggcgttcaa catccgcaga cgttcggcaa
      121 aatccgcaaa cggcagcagc tccgaaccga aacggcgcat atgttccgta tcctgctggc
      181 agtctatcag ttccacgccc aaatccgcca aaaacggcac ggcgcaggca aacgcgattt
      241 tcgacgcatc cggttgcaac gcaaacatcg attcgccata aaacaccctg ccgatctgaa
      301 cgccgtaaaa gccgcccgcc aacctcgttt taccgttttc atcgggataa tggcactcga
      361 aagaatgcgc gtgtcccatt tcgtgcagct tcaaatatgc cgtctgaaac tcgggcgcaa
      421 tccaagtccc atcctgattc gggcgcgccg ctgccgcaca atgcgcgaca acttccgcaa
      481 aacagccgtt gaccgcaacc cgatagctgc cgttacgcag cgttttcgcc agcgagcgcg
      541 gaacgtgcag acgttcggga acaattaccg cacgcggccc gaccgcatac caaaaaaacc
      601 aaccgtcccg cgaaaaccaa ggaaacacgc cgttccgata cgcctcgagc agccgccccg
      661 cgtccaaatc cctgctcacg ccgaccagcc cgtcgcaccg ggccaaagca taggcaggat
      721 cgggaaaggc ataattgtca ggggcaagca gcggaatacg catggcagtt ctccaaaaac
      781 acagacggca tccaaaatca tgaatgccgt ctgaacaaca aaccgtccgg atttaacgtt
      841 tgcccttggc ctgacagtcg ctgcacacgc cgtacatata aagcgcgtga tcgacgatgc
      901 ggtagccgtt ttcctcggcg attttgtctt gcagggcttc gatttcggga ttgtggaatt
      961 ccgttacctc gccgcacttc acgcagacga tatggtcgtg gtggtcgccc ttgtccaact
     1021 cataaaccgc cttgcccgtt tcaaaatggt ggcgttgcag aatgcccgcc tgctcgaact
     1081 gcgtcagcac gcggtaaatc gtcgccacac cgatttccac gccctcttcc agcagaatgc
     1141 ggtacacatc ttccgcactc aaatgctctt ccgcgtgctt ctcgaacaaa tccaaaatct
     1201 tcaaacgcgg gccggtaacc ttcagaccgc tgtctttcag ttgcgcaatg ttgctgaatt
     1261 tttccataat attcaatatc cctgtaaaat aatagacgct ataatacgca atttcagcct
     1321 gcttgcccac tatcacacca tagcagttcg caatagcaaa accgcaacgg cggacgggca
     1381 gtcggacaca gacagggtaa aataccgctt aagcgtatga ttatcgttcg cttttataaa
     1441 atattcaagc agtgctacac tagacatccc gatttgcaca gaaaggttct cccgtgaaca
     1501 aaaccctcat cctcgccctt tccgccctgt tcagcctgac cgcgtgcagc gtcgaacgcg
     1561 tctcgctgtt tccctcctac aaactcaaaa tcatccaagg caacgaactc gaaccgcgcg
     1621 ccgttgccgc cctgcgcccc ggcatgacca aagaccaagt cctgctcctg ctcggcagcc
     1681 ccatactgcg cgacgctttc cataccgacc gctgggacta taccttcaac acctcccgca
     1741 acggcatcat caaagaacgc agcaacctga ccgtctattt tgaaaacggc gtactcgtcc
     1801 gcaccgaagg cgacgccctc caaaatgccg ccgaagccct ccgcgcgaaa caaaacgcag
     1861 acaaacaata aggaacacac atgataccgc tcaaaatcgc catcgccggc gcaaacggcc
     1921 gcatgggacg cgtcctcgtc gaagccgtca acaaccatcc cgacaccgtc ctttccggcg
     1981 cgctcgaaca ctcaggctca gaagccctcg ggctggacgc gggctacgcc gtcggactca
     2041 aaaccggcat cgccatttca gacgacgttg acgccgttct cgcccaaagc gacgtactca
     2101 tcgacttcac ccgccccgag ccgaccctca agcacctgca aaaatgcgtt gaaaaacaag
     2161 tcaacatcat catcggcaca acaggcttcg acgatgcggg caaagccgcc atccgcgccg
     2221 ccgccgaaaa aacaggcatc gttttcgccg ccaacttcag cgtcggcgtc aacctcacct
     2281 tccacatcct cgacaccgtc gcccgcgtcc tcaacgaagg ctacgacatc gaaatcatcg
     2341 aaggccacca ccgccacaaa gtcgatgccc ccagcggcac agcattacga atgggcgaag
     2401 tcatcgccgg cgcgctcggc cgcgacctca aacaatgcgc cgtttacggc cgcgaaggcc
     2461 acactggtcc gcgcgatcct tccaccatcg gctttgccac cgtccgcgca ggcgacatag
     2521 tcggcgacca caccgccctc ttcgccaccg acggcgaacg cgtggaaatc acccacaaag
     2581 ccggcagccg catgaccttt gccgccggtg ccgtccgcgc cgcagtttgg gtaaacggca
     2641 aaacgggttt gtacgatatg caggacgtac tcgggctgaa caaccgttaa cccccataca
     2701 aaatgccgtc tgaaaagata ttgttcagac ggcattttgc cgacaggctt cgtatcggca
     2761 tatcaataaa gttgcttgtg cagcatgaca agctacggct cgtttaattg aaactctcct
     2821 gatctaaaaa ttccaactct atttcccggc aaactatatc tatgataaaa ctggtcggaa
     2881 gattatacct acgccttatt aatgccagtg cacctccata atccaagatt tcatcatcct
     2941 gctttaattc tccaagctcc aaggtattat cgatatcttt atcttggttc tccgtagcgt
     3001 atccatagat aaatttcaaa tagctgaact gcttgaattt aatttttacc cagtctgtgt
     3061 aatttggtgt aggacacaat tgcctaaaat agaaatttac gaagaacaaa tgaaacaagg
     3121 tacgtaagcg tattagtata aactataatc atatataata aaattaatca gttaacgaac
     3181 cattatattt taaccttaat cccattcggg ccagttctgt ttcatttgga attcctctta
     3241 aatacttatt gtttgatgtg ccattaatca caatttctcc cccttttttt gtaatacgtg
     3301 ctgcttcggg aaaataatcc attaaccgct caacatcccg cctcttccaa aaccgcacgc
     3361 gcggccaaat ccgccgtatc ttttttcaac agcaggtgca acgccccgaa atcctgttgc
     3421 agcgcggcaa ccttatcggg gtgttcgtac cagtccgcca atgccgccgc cagtttttcc
     3481 ggttttgctt cagattgcaa taattccggc acggcctcct tacccaacag gatattcggc
     3541 aggccgacat gcggcacttt gattttgcgt ttcacataag cataggtcag cggcgaaatc
     3601 ttgtagctga tgaccatcgg acgcttgcac aacgccacct ccaaagtcgc cgtaccgctc
     3661 gttaccagca ccgcatccgc cgccctgcac accgtttcag actgtctgtc ggttaccgtc
     3721 agcgccaatc cggcaaactc cggccgctgc aaaacttccg ccaaacgccg cttcgtcgcc
     3781 tccgttgcgg caggcagcag gaagcgggcg gcgggatagc gttccaacaa caataatgcc
     3841 gtctgaaaaa acaccggcgc catatagtcg atttcgctga cgcggctgcc gggcagcagg
     3901 gcgaatacgg ggatgccgac atccgcgccc aaagttttcc gcgccgtttc acggtcgtct
     3961 tccaagggca taagctgtgc catcggatga ccgacaaact ccgcacgtcc gcccgcatcg
     4021 agataaagct gcggctccat cgggaacagg cacagcacgc ggttgacctg atgcacgatt
     4081 ttgcccacgc gttcgcgccg ccacgcccaa accgacggac tgacataatg cagggtcgga
     4141 atgcccgccc gtttcagctt ttccgccaca cccagattaa aatcgggcgc atcgataccg
     4201 acaaagacat caggtttcaa cgacagcaaa tcccgtacca gctccctgcg tatccgtaaa
     4261 atttccggca gccgcctgac cacttcgaca aagccgcgca ccgccagccg ctcctgatca
     4321 taaaggctct cgaaaccttc cgccttcatc agttccccgc cgatgccggt taaccgtgcc
     4381 tgcgggcaac gctttcggat ggcgcgtatc aggtgcgccc ccaacaggtc gcccgacgct
     4441 tcgccgacac tgacggcaat caaagggctt tttttatcaa ccatattcgt ctgtccccac
     4501 atatagtgga ttaacaaaaa ccggtacggc gttgcctcgc cttgccgtac tatctgtact
     4561 gtctgcggct tcgccgcctt gtcctgattt ttgttaatcc actatacttt cacgccccga
     4621 tgccgtctga agccttcaga cggcatcggg gttcacattt tcgtcccgtt ttccaacatc
     4681 accgcaaact gcgccaaatc cggcggcggc tccgccttca acaccagcgg ctcacccgtg
     4741 agcggatggt tcaggtgcag ctcggacgcg tgcaaaaaca tgcgcttcaa acccaatttc
     4801 tgcaaacgcc ggttcgcctg ataatcgccg tagcgttcgt cgcccgcaat cggacagcct
     4861 tgagattgca gatgcacgcg gatttggtgc gtacggcccg ttttcagcgt tgcccgcacc
     4921 aaagtcaggt gcgacagccc gacaccgtgc aaaatgccgt ctgagaaacg gcttaacaca
     4981 cggaacaccg tatgcgccga ctgcccgtcc tcgctgacgc gtaccatctt ttcgccttgt
     5041 gcgccggtat atttgaacag gggcagtttg acatggaaat tgtcgtccgg cagtttgccc
     5101 acccccagcg caaggtagat ttttttgggg tggtcgttac ggatagcttc gtgcagtttg
     5161 acgagcgcgc tgcgtttctt cgccaccatc aacaagccgc tcgtatcctt gtccaaacgg
     5221 tggaccaact ccaaatactt cgcctccgga cgggcgcggc gcaactgttc gataacgccg
     5281 aaactcacgc cgctgccacc gtgtaccgcc acaccggaag gcttattgac caccagtagc
     5341 gcgtcgtcct catacaccac ctcaaacgca cgcgccggta cggcggcacg cctttcagac
     5401 ggcatttcct tctccgccac gcgcacaggc ggaatccgca ccgtatctcc ctccgcaata
     5461 cggctgtcgg gtttgcagcg tttcttgttc aaccgcacct cgccggcgcg gataatgcgg
     5521 tggatatagc ccttgggcac gcccttgagg atttttatca gatagttatc aaggcgttga
     5581 cccgcctcat gttcggcaac ccctatcagg ctgaccgaac ctttgcttat ttcgtgcgtt
     5641 ttcatctata atccgaacat ccgtttcagc agaaagcgcg tccgccgcgc cccctgaaac
     5701 ggtttacttt aaaacggaat tttatattaa aaacgcactc cgcaaagcat tttccctgcg
     5761 cctgttccca gccggcaggc gcagaacgta gtcaagtttg aattgatttt gccgtttcgg
     5821 cgcggaagta agacggcagc ggagccccaa gccccgaacg caaaccgccc gaaaacacag
     5881 gcatcacgat aacaagaaga agccggcgcc catttttccg accgcagcgt tcggaacgcg
     5941 gcaggcagac cgccgcatcg atctttatag ccttctttat ccgacccttc cgcacacggt
     6001 tccccggaac gtgcatccct gcggattttc aactcaaaaa ctgattaccg gttttatccg
     6061 aagacatcgg aaacccagcc ttacggaatg ccgaaccgga ccggcgcgcc cgaatgccgc
     6121 ctgtccacga ggtgatcatg aaaagaatgt tattcaacgc aacgcaggcc gaagagctgc
     6181 gcgttgccat cgtcgacgga caaaacctgc tggatttgga catcgaaacg ctgggcaaag
     6241 aacagcgcaa aggcaatatc tacaaaggca tcattacccg catcgagccg tcgctggaag
     6301 cgtgtttcgt cgattacgga accgaccgcc acggcttttt gccgtttaaa gaagtatcgc
     6361 gttcgtattt cctcggctac gaaggcgggc gcgcgcgcat tcaggacgtg ctcaaagaag
     6421 gcatggaagt catcgtccaa gtcgaaaaag acgagcgcgg caacaaaggc gcggcgctga
     6481 ccaccttcat cagcctcgcc ggacgctatc tggtattgat gccgaacaat ccgcgcggcg
     6541 gcggcgtatc ccgccgtatc gaaggcgaag agcgtcaaga actcaaagcc gcgatggcgc
     6601 aactcgacat tccgaacggc atgagcatca tcgcgcgtac cgccggcatc ggccgcagcg
     6661 cggaagagtt ggaatgggat ttgaactacc tcaaacaact ctggcaggca atcgaagaag
     6721 caggaaaagc gcatcacgac ccctacctgc tctttatgga aagctcgttg ctcatccgcg
     6781 ccatccgcga ctacttccgt cccgacatcg gcgaaatttt ggtggacaat caagaagttt
     6841 acgaccaagt tgccgagttc atgagctacg tcatgccggg caacgcaggt cgtctgaaac
     6901 tctacgaaga ccacacgccg ctgttttccc gcttccaaat cgaacaccag atcgaaagcg
     6961 cgttctcgcg cagcgtcagc ctgccctccg gcggcgcgat cgtcatcgac cacaccgaag
     7021 ccctcgtctc catcgacgtg aactccgcac gcgccacacg cggcgcggac atcgaagaca
     7081 ccgccttcaa aaccaatatg gaagctgccg aagaagtcgc ccgacaaatg cgcctgcgcg
     7141 acttgggcgg cttggtcgtc atcgacttca tcgacatgga aaaccccaaa caccagcgcg
     7201 atgtggaaaa cgtcctgcgc gacgcactca aaaaagaccg cgcccgcgta cagatgggca
     7261 aactctcccg tttcggactt ttggaattga gccgccaacg cctgaaaccc gctttgggcg
     7321 aaagcagcca cgccgcctgt ccgcgctgcg ccggcaccgg cgtgatccgc ggcatcgaat
     7381 ccaccgccct gcacgttttg cgcatggttc aggaagaagc gatgaaggac aacaccggcg
     7441 aagtgcgcgc ccaagtgccc gtcgatgtcg ccaccttcct gctgaacgaa aaacgcgccg
     7501 agctgtttgc aatggaagag cgtttggatg tgaacgtcgt cctgattccg aacatccacc
     7561 tcgaaaatcc gcactacgaa atcaaccgca tccgcaccga cgacgtagaa gaagacggcg
     7621 aaccgagcta caagcgcgtt gccgagcctg aagaagacga atccgccaaa cctttcggcg
     7681 gcgaaaaagc caaagccg
//
LOCUS       DAWTQS010000077         7573 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.077, whole genome shotgun sequence.
ACCESSION   DAWTQS010000077 DAWTQS010000000
VERSION     DAWTQS010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7573
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.077"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(95..301)
                     /locus_tag="WNA25_001847"
     CDS             complement(95..301)
                     /locus_tag="WNA25_001847"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFA9824542.1"
                     /translation="MARITTEDCTGKISNHFDLTLVAARRARQLENGNTPLVDDVRNN
                     KPTVTALREIAAGHIGTELLTRNK"
     gene            complement(359..976)
                     /gene="gmk"
                     /locus_tag="WNA25_001848"
     CDS             complement(359..976)
                     /gene="gmk"
                     /locus_tag="WNA25_001848"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFA9824543.1"
                     /translation="MSAYRKGNIFIISAASGTGKTTLVSRLLANHNGLRVSVSHTTRP
                     PREGEANGVHYHFVSKEEFESLIAQEAFLEYADVFGNYYGTSTEGVNALAAAGYDVIL
                     EIDVQGAAQVRNALPEAVGIFILPPSFDVLAARLKGRGTDSREVIQRRLSKARHEIEQ
                     SVLFDFVVVNDDLEKAEGDLLHIVNACRLKRSRQLGFIADLLENS"
     gene            1104..1703
                     /locus_tag="WNA25_001849"
     CDS             1104..1703
                     /locus_tag="WNA25_001849"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFA9824544.1"
                     /translation="MALKTSNLEHAMLVHPEAMSVGALADKIRKIENWPQKGILFHDI
                     TPVLQSAEYFRLLVDLLVYRYMDQKIDIVAGLDARGFIIGAALAYQLNVGFVPIRKKG
                     KLPFETVSQSYALEYGEAAVEIHTDAVKPGSRVLLVDDLVATGGTMLAGLELIRKLGG
                     EIVEAAAILEFTDLQGGKNIRASGAPLFTLLQNEGCMKG"
     gene            complement(1749..2537)
                     /locus_tag="WNA25_001850"
     CDS             complement(1749..2537)
                     /locus_tag="WNA25_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFA9824545.1"
                     /translation="MNPKIVFFDIDDTLYRKYTDTLRPSVKTAVAALRGKGILTALAT
                     GRSLATIPEKVRDMMAETGMDAVVTINGQFALLHGKTVREVPMDTGLMGRVCAHLDGL
                     GMDYAFVGGEGIAVSALSECVCRALQHIASDFFADKDYFSSKPVYQMLVFAEENEMPL
                     WSDIVEREGLKTVRWHEEAVDLLPAGMSKTDGIRSVVEALGLEMADVMAFGDGLNDVE
                     MLSEVGFGVAMGNGEQAAKEAAKYVCPGVDEDGVLRGLQDLGVI"
     gene            2863..4218
                     /gene="yegQ"
                     /locus_tag="WNA25_001851"
     CDS             2863..4218
                     /gene="yegQ"
                     /locus_tag="WNA25_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFA9824546.1"
                     /translation="MKAPELLLPAGGLERMRAAYDYGADAVYAGSPRYSLRARNNEFA
                     KLDVLEQGIKEAHERNKKFFLTVNTLPHNSKLKTFVADMEPLIAMKPDALIMADPGLI
                     MTVREKWPEMPIHLSVQANTTNYWGVKFWQNIGVERIILSRELGMEEIAEIRQECPDI
                     ELEVFIHGALCIAYSGRCLLSGYFNHRDPNQGTCTNSCRWDYKVHNATESEAGDAQLL
                     QGFNFEKAQEEANQNFEGINGQKRHPYADKVFLIEESNRPGEMMPIMEDEHGTYIMNS
                     KDLRGIEVVEKLAKIGVDSLKVEGRTKSLYYVARVAQSYRKAIHDAVAGRPFDYSLLS
                     ELEGLANRGYTSGFLERHQTQDYQNYLSGHSTAKQSQYVGHVTEIDENGWATIEVKNR
                     FAVGDSLEIIHPSGNQTIKLEQMTRKGQPVDVAPGNGIQVKIPNMQGKEKALIARVLN
                     P"
     gene            4349..4822
                     /locus_tag="WNA25_001852"
     CDS             4349..4822
                     /locus_tag="WNA25_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease"
                     /protein_id="HFA9824547.1"
                     /translation="MLAACQSGRALKNAHTASAGTPAGESCKVIRGDTAGGGKNIIYR
                     CSNGQAAINAATAAGVLESGIPVSFGGGVPVANGRNLVTRQAANGVGKSDSEACEHAL
                     INAARKFQQTAGKLGGRSVTGFHSYFGKQSLQGGQYDCQAGSFHVRAVMRANVVR"
     gene            complement(5031..7404)
                     /locus_tag="WNA25_001853"
                     /pseudo
     CDS             complement(5031..7404)
                     /locus_tag="WNA25_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222141.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
BASE COUNT         1873 a         2150 c         1780 g         1770 t
ORIGIN      
        1 atttcagacg gctttcggga tgtatgtgtt ttttcatttc aaaccgtcgg actgcacggc
       61 ggcagagtgt gccggcgtgc gtttccggca gaatttattt attgcgcgtc aacagttccg
      121 taccgatatg cccggctgcg atttccctta aggcggtaac ggtcggtttg ttattgcgga
      181 catcgtccac aagcggcgta ttgccatttt caagctggcg ggcccgacgg gccgctacca
      241 atgtcaggtc aaaatggttg gaaatttttc cggtacagtc ttcggtggta atacgtgcca
      301 tattatttgc tttctttcaa aaatatttaa attgggaaac cggatatttt cgcggttttt
      361 aggaattttc caacaaatct gcaataaatc ccagttgccg cgaccttttc agacggcagg
      421 cattcacaat atgaagcaaa tccccctccg ccttctccag gtcgtcattg accacgacaa
      481 agtcgaacaa tacggactgt tcgatttcat gccttgcctt cgacagcctc ctttggataa
      541 cttcccgact gtccgtcccg cgccctttga ggcgcgcggc aagcacgtcg aaagaaggcg
      601 gcaggataaa gataccgaca gcttccggca gcgcgttgcg aacctgcgcc gcgccctgaa
      661 cgtcgatttc caaaatcacg tcatagcctg ccgccgccaa cgcattcacg ccctccgtgc
      721 ttgtgccgta atagttgccg aatacgtctg cgtattccaa aaaagcctcc tgcgcgataa
      781 gcgattcaaa ctcttctttg gaaacaaagt gataatgtac gccgtttgct tcgccttcac
      841 gcggcgggcg cgtcgtatgc gacacggaaa cgcgcaaacc gttatggttt gccaacagcc
      901 gcgacaccag cgtggttttg cccgtgccgg aagcggccga aatgataaag atgttgcctt
      961 ttcgataagc ggacatattt tttacctgta tattttccaa ccgattgtat cacaacggac
     1021 accctatttc atatttgccg atgcccatat tttgccgcta ttgttttgat tgatttggca
     1081 agcggcaggc tgacggctac aatatggcgt taaaaacatc aaacttggaa cacgcaatgc
     1141 tggttcatcc cgaagctatg agtgtcggcg cgcttgccga caaaatccgc aaaatcgaaa
     1201 actggccgca aaaaggcatc ttattccacg acatcacgcc cgtcctgcaa agtgcggaat
     1261 acttccgcct tttggtcgat ttgctggttt accgctatat ggatcagaaa atcgacatcg
     1321 ttgccggctt ggacgcgcgc ggcttcatta tcggcgcggc actcgcctac cagctcaacg
     1381 tcggcttcgt ccccatccgc aaaaaaggca agctgccttt tgaaaccgta tcgcaaagct
     1441 acgcgctcga atacggggaa gctgcggtgg aaatccacac cgatgccgtc aaacccggtt
     1501 cgcgcgtcct gctggtcgat gatttggttg ccacgggcgg cacaatgctt gccgggctgg
     1561 aactgatccg caaactcggc ggggaaattg tcgaagccgc cgccattttg gaatttaccg
     1621 accttcaagg cggcaagaat atccgcgcaa gtggcgcgcc cttatttacc ctgcttcaaa
     1681 acgaaggctg catgaaaggc tgaaaaccga ccctgccgtc tgaaaccggc agggttgtta
     1741 ttatacgttc aaatcacacc caaatcttgc aagcccctca acacgccgtc ttcatcaacg
     1801 ccggggcaaa catatttcgc cgcttctttc gctgcctgtt ccccgttacc cattgccacg
     1861 ccgaacccga cttcagacag catttccaca tcgttcaaac cgtcgccgaa cgccatcacg
     1921 tctgccattt ccaaacccaa tgcttcaacc acgcttctga tgccgtctgt tttcgacatt
     1981 cccgcaggca gcagatcgac cgcttcctcg tgccagcgca ccgttttcaa gccttcccgt
     2041 tccacaatat ccgaccaaag cggcatttcg ttttcctccg caaacaccag catctgatac
     2101 accggtttgc ttgaaaaata atccttatcg gcaaaaaaat cgctggcgat atgctgcaag
     2161 gcgcggcaca cgcattccga cagcgcggac acggcgatcc cctctccgcc gacaaacgca
     2221 taatccatgc ccaagccatc caaatgcgca caaaccctgc ccatcaaacc ggtatccatc
     2281 ggtacttcgc gcacagtttt accgtgcagc agcgcaaact gtccgtttat cgttaccacg
     2341 gcatccatac ccgtttccgc catcatgtcc ctgacctttt cgggaatcgt cgccaaagac
     2401 cgccccgttg ccaacgccgt caatatacct ttgccgcgca aagccgccac cgccgttttc
     2461 acggaagggc gcaaggtatc cgtatatttg cggtacagcg tatcgtcaat gtcgaaaaac
     2521 acgattttgg gattcatcat attctctctc gcactcaaac tgccgcattg tatcccaaga
     2581 aggcaaatac ttgataaatc cttataaatt tcccggcaaa attgaccgcg aacacgaaaa
     2641 ggcggataat ccgcccatct tcaaacaccc ttcagacggc atttgcagca atgccgtctg
     2701 aaacattttt acaaagcata caaatcatgc ttcaacacac aggacgacac ataaagcacc
     2761 gccctattat gtcgtcctga ttcggaaggg gttacgcccc tcccaaacaa agtctgatcc
     2821 tgccgcccta aagggcgggg tttcaaccga aaaggaaaca cgatgaaagc acccgaactc
     2881 ttattgcccg ccggcggatt ggaaagaatg cgcgccgcct acgattacgg cgcagatgcc
     2941 gtttacgccg gcagcccgcg ttactccctg cgcgcccgca acaacgaatt tgccaaactc
     3001 gacgtcctcg agcaaggcat taaagaagcg cacgaacgca acaaaaaatt ctttttgacc
     3061 gtcaacaccc tgccgcacaa ttccaaactc aaaaccttcg ttgccgacat ggagccgctg
     3121 attgccatga aacccgacgc gctgattatg gcggatccgg gtttgattat gaccgtgcgc
     3181 gaaaaatggc cggaaatgcc gatccatctg tccgtacagg cgaacaccac caactactgg
     3241 ggcgtgaaat tttggcagaa cattggtgtc gaacgcatca tcctgtcacg cgaattgggc
     3301 atggaagaaa ttgccgaaat ccgccaagaa tgccccgaca tcgaactcga agtcttcatc
     3361 cacggcgcat tgtgcatcgc ctactcaggc cgctgcctgt tgtcgggcta tttcaaccac
     3421 cgcgatccca accaaggcac ctgcaccaac tcctgccgtt gggactacaa agtccacaac
     3481 gccacggaaa gcgaggcagg cgatgcccag cttctgcaag gtttcaactt tgaaaaagcc
     3541 caagaagaag ccaaccaaaa ctttgaaggc atcaacggtc aaaaacgcca tccctacgcc
     3601 gacaaagttt tcctgattga agaatccaac cgcccgggcg agatgatgcc gattatggaa
     3661 gacgaacacg gcacctacat catgaactcc aaagatcttc gcggtatcga agtcgtcgaa
     3721 aaactcgcca aaatcggtgt ggacagcctc aaagtcgaag gccgtaccaa gtctctctac
     3781 tacgtcgccc gcgtcgccca gtcctaccgc aaagcgattc acgatgccgt cgcaggccgt
     3841 ccgtttgatt acagcctgtt gagcgaactc gaaggccttg ccaaccgagg ctacaccagc
     3901 ggcttcctcg aacgccacca aactcaggat tatcaaaact acctcagcgg ccattccacc
     3961 gccaaacaaa gccaatacgt cggacacgtt accgaaatcg acgaaaatgg ctgggcgacc
     4021 atcgaagtta aaaaccgctt cgccgtcggc gattcgctcg aaatcatcca cccgagcggc
     4081 aaccaaacca tcaaattgga acaaatgacc cgcaaaggcc aacctgtcga tgttgccccg
     4141 ggcaacggca ttcaggtcaa aatccccaat atgcagggca aagaaaaagc cctcatcgca
     4201 cgcgtgttga acccctgagc cacaatgccg tccgaacccc gttttcagac ggcattttgt
     4261 tgaatcttac tgtacaatgg caatgatttc ccacagcaaa caaggacatc cgacataaaa
     4321 aatcctcata ctctccgccg ccgccctgat gctggcagcc tgccaatccg gccgcgcgct
     4381 taaaaacgcg cacacggcat ccgccgggac accggccgga gaatcatgca aagttatccg
     4441 cggagacacc gcaggaggcg gcaaaaacat catttaccgt tgtagcaacg gtcaggcggc
     4501 aataaatgca gccaccgccg ccggcgtatt ggaatcaggg attcccgtca gcttcggcgg
     4561 cggcgtaccg gttgcaaacg gcaggaatct agttacacgg caagcggcaa acggcgttgg
     4621 caaatccgac tccgaggcct gcgaacacgc cctaatcaat gccgcccgca aattccagca
     4681 gaccgccggc aaactcggcg ggcgcagtgt taccggcttc cacagctact ttggcaagca
     4741 atcgctgcaa ggcggacaat acgactgtca ggcaggttcg ttccacgtcc gcgcggtcat
     4801 gcgcgccaat gtcgtccgtt aacatatcgg caatcaaaaa atgccgtctg aaacattttt
     4861 cagacggcat ttttaatcct gcaacattac tccctgccct gagttcggat actgtatcaa
     4921 tataaaaccc catcacacag atttacggta aaaagccgtc cgaatgagtt cctgaaaaca
     4981 attcggacag ctttaatccc caacgaggca attaatccaa taatactaga ttaaaacttc
     5041 cattccagcg atacggcgta attacggcct gaggcgcggt agcggtctaa gcctttgcca
     5101 tctcggtcga ccgcgttggt ggtgctgtag ctatacaaac cgcgcaggga atcccaagtg
     5161 gtgtatttgc ggttgaacac attatatacg cctgcacgca aagtcaggtt tttagccggt
     5221 ttgtagaagc cgtacatatc aaacacataa gccgacttgt tcagccacgg gtaatctttt
     5281 acctttttct gcaaaggcgt accccggccc ttgttttcat aaacggtgta ttgcgcgtct
     5341 ttggcctttt tcgcacccag ataagtcagg cgggagaaca caccccattt ttcgctcggg
     5401 cttcgtagtc gacaccggca atcactttcg gcggctgtgt ggacagcagg ctgttgtcgc
     5461 ccgacagttt gcttttcgca taacccagcg agccgaacaa tttccagccc tcaggaacaa
     5521 aagacgctat tttgtccaca ttcagacggc ctgtcagctc aagaccacgg attcgggcct
     5581 tatcgatatt ttgcatctgc cattccggtt tttcggaata aggattgcta cacataccgt
     5641 agtagtaatt catctgagta cagccgacat cgccgctggt ggtcagcttc tgctcttcag
     5701 acaagaagtt gcggtaattg ttttgataca ggttggcatc caaagtacct ttttcgctgc
     5761 ggccttgcag agacagggtg tgggtggtgc tgcgctcggc tttcaggttg ggattgggca
     5821 gccaattacc cgaaccgtgg ttgtaagtga aatacacttc ggacgcattg gggacacggt
     5881 agccggaagt aatgtcgtaa ccgacatgcc aagcctgatt cagttgcgcc gccaaaccga
     5941 caaatccgct ccagccttta taagtattgg ctgcaggcgg tgttttgtca caagcatgac
     6001 actcggcatt caattcctga ggcgtcattt tggtatgatc gtaacggata tctgcacggc
     6061 tgctgaacac gtcgttccat tggatttgat cagacagtga gaaaccataa ttagtggttt
     6121 tcacagggtg ttgaatcgag ctggtagtac gggatatctg tccgctgaag taataatcgt
     6181 cgcggtttaa gttttcaaac tcacgccgac tggcgaaagt tttaaacgac aagcgatgtt
     6241 ggccgcccag ttgcaacggt tggctgtcca tacgcaaagt aaaacgtttg aatcgggtgt
     6301 ccatgctgcg gttgtatatt tcgtccaaat ccttctgatt tatagttgcg cgtccaggtg
     6361 gaataatcca tcgggaacga gcctttgttg ttaaccgccg ccactttggt tttctgataa
     6421 tcgaagtccg ccttcaaaga cgacaaccaa tttgaatcag gcgtccattc gtaaaagagg
     6481 ttggcattgc gccgtctgtt tacgtcatcg gcttcgcgcc aggaagaagc ggtcaggtta
     6541 taagactctt caaatcgtgt aattatgccc ctgctggccg ttaaacgatg cgccgatgcg
     6601 gtgcttgtcg ttgatttgat aagcaatctt acccaagaag ttgtggtatt tgtgtttgga
     6661 cggatcaggg ataccgcgtg ccgaaccacg gatattcgct ccgctgccag caccctctac
     6721 cggatagcca cgctcgcccg cgctttcggt ctcatgaccg caacgttgcg aatacagcaa
     6781 agcggcatcc acgcggtcgt tgctcacacc gaaaccgagt gtatttgtcc attcgcggtt
     6841 gcggctgctg taaccgtttt tcatcatcac gccgaattgc ctgtcgtcca acagcaaatc
     6901 atggccttgc agggtttggt aattcacgcc gccacccaat gcgccgctac cggtattgaa
     6961 agagtcagcg cccttcgcga tttcgatgtt gcgcacgagt tcggggtcga tagacaggcg
     7021 cgagctgttg aagttgccat aacgtgcata cagtgagttt tcttccgaat caggcaggct
     7081 cacgccgtca atgctgacac cgacacggtt gccttccacg ccgcgcacag caaagccttt
     7141 ttgatggcgg ccgctatcgc tcaagccgac gtcggtggag taacgcacca agtctttgtt
     7201 gtcgcgtatc atttcctgtt gaatgcggcc gaggttgaca cgttccacgg ttgcaggcgc
     7261 attaagctgg tctttaacgc gcacttcttt tatctctgct ttaacgggtg tggtttcggt
     7321 tgcagcttca tccgctgcca agaccggatt gccgaaaata ctgccgacca gcgcggcaat
     7381 aggaagcatg tgtaatggtt tcatattatc aactcaagat gtaatggatt gttcatccat
     7441 cagttaattc ataataaatt ttctgataat cattaatatt taataagaca gcaacccgta
     7501 caaataaatc tgtgtcgtgt aattaaaggt ctctgcaaac atctatgccg aggccttgtt
     7561 tgtttcatta ttt
//
LOCUS       DAWTQS010000078         7498 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.078, whole genome shotgun sequence.
ACCESSION   DAWTQS010000078 DAWTQS010000000
VERSION     DAWTQS010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7498
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.078"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            243..1025
                     /locus_tag="WNA25_001854"
     CDS             243..1025
                     /locus_tag="WNA25_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824548.1"
                     /translation="MKLRRLIMEMQLDKQTLTVVVRNSEPVELSVFAQSMMSLADDYE
                     SMCGSAGSHARLYIKEIRQGSIIAELAPLLPLAGTLFEGAGQILAYAKNFIEITDWLM
                     GKGKEPAGVTDSQIRNIANIMQPAASDKNGSIEINVNDNNGSVVNNITYNYFAANTVQ
                     NQARRILGERAEASESGDYGQMVMYFVQAAPTKETNQAVIEGIYSRPVKILIPEHIKR
                     EMFAEPYPFEKYYIVDVSVQTARGKPRLYKVTGYHGVVDGDD"
     gene            complement(1124..1525)
                     /locus_tag="WNA25_001855"
     CDS             complement(1124..1525)
                     /locus_tag="WNA25_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFA9824549.1"
                     /translation="MFIPAALHKDEHSAYGVTIPDLPGCFSCGDTVEEAVANARSAAY
                     MHIDGMIEDGGFKNLAVSSIADLSQEPDYHGATWVMIEIDPAKISRQQIRFNVSWPQY
                     LLDRVDEYTSANHETRSGFLAKAALLTMNQA"
     gene            complement(1627..1809)
                     /locus_tag="WNA25_001856"
     CDS             complement(1627..1809)
                     /locus_tag="WNA25_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFA9824550.1"
                     /translation="MNSLDVIALLKQDGWYKVAQSGSHSQYKHPTKKGRVTVPHPKKD
                     LPTGTVKNIYKQAGLK"
     gene            complement(1979..2797)
                     /locus_tag="WNA25_001857"
     CDS             complement(1979..2797)
                     /locus_tag="WNA25_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HFA9824551.1"
                     /translation="MNQYAMRFAVIRFMPYVQTREFANIGIIITHPKSGYFDFKIEQR
                     YSRLSRFFRRFDSSVYKAATRAFAEELQRIGKLAAYSAPDQIRAMLDHLTRPREALIM
                     AAQPGVTLAPDRGQELNRLFDYFVARSFAKSQPEAELTRQIQAMLKPLQTAYPFKEST
                     IGDPSGFHASIPLVQKAENGEIRKIIKPIYFGQKDPADIYYKSDKWIAGIKRLRRGGY
                     IDRSEILFAYEPPERPDKAQEKALLDVLGDLEEQGIRLADNRSEGKIIRNFACG"
     gene            complement(2794..3492)
                     /locus_tag="WNA25_001858"
     CDS             complement(2794..3492)
                     /locus_tag="WNA25_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824552.1"
                     /translation="MDRAEQGVTSPFICMAENGLEYFVKGLHATRASQINEWIGGNMA
                     QALGLPVAPFELLEVGEELYEELPTKMKEIGKGICFGSLAQKGCALLEPADIPRIDTV
                     MQRQIAAFDWFVRNEDRTIGNPNLLYRNCNNSLIVIDHNCAFDTGFNPNNFLQNHIFS
                     SAFRQILEDWVLREEMELWLKSALPAYRKACDNLPPEWAWANEERDLPAAYNRSRTDE
                     TVRRIDNGTLWSIS"
     gene            complement(3731..4429)
                     /locus_tag="WNA25_001859"
     CDS             complement(3731..4429)
                     /locus_tag="WNA25_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFA9824553.1"
                     /translation="MNRVDRIKNLIADRFNGNQAEFSRAINKAPAQINQWLNGYRNIG
                     DGVAAQIETALGLPRGWVDGKDEPNIPIDSIKSNATVIGTVDAWDSKTPLSDDDCEVP
                     FYKDVCLSAGNGFSDEIEDYNGYKLRFSKSTLRRHGISPDDVVCVSADGDSMEPVFPD
                     GATLGINTADKMIKDGKIYAINHSGLLRTKILQKLPDNQVRIKSYNPEYKDETASLDS
                     LTVIGRVFWWSVLD"
     gene            complement(4469..5122)
                     /locus_tag="WNA25_001860"
     CDS             complement(4469..5122)
                     /locus_tag="WNA25_001860"
                     /inference="COORDINATES: protein motif:HMM:NF012920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFA9824554.1"
                     /translation="MKNLVEEAGSVAELAKRAGYAQPSYLYQIINQTAIQNGKPKNIG
                     GAMARKLESAMNKAEGWLDMNHGETLPLPPEDTETIRIDHLDMAGACGGGIEADDWPS
                     PISSVEFPLDAVRRLFSGRDTSGLKIVGTRGDSMEPTIPETSAVLIDTKADTFAGDGI
                     YFFAYAGGLYTKRLQKTPAGLLALSDNPLYQSFPIDENDRFRIIGRYYGVISLKLAQ"
     gene            5320..5505
                     /locus_tag="WNA25_001861"
     CDS             5320..5505
                     /locus_tag="WNA25_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFA9824555.1"
                     /translation="MTRLAAAINEKLSNLSSMRHGKKTVPPYKCRRIVEATNGEVTLK
                     DLRPDDWHEIWPELKED"
     gene            5507..5707
                     /locus_tag="WNA25_001862"
     CDS             5507..5707
                     /locus_tag="WNA25_001862"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824556.1"
                     /translation="MEIVPVKWEDAFGCPEGWQMLDGVGRETSLVQSVGFVAAETETT
                     LTIVPHVGGLNRERGRLLERVY"
     gene            5904..6710
                     /locus_tag="WNA25_001863"
     CDS             5904..6710
                     /locus_tag="WNA25_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFA9824557.1"
                     /translation="MKYIPNSFQIANAVVDDFLCRMSGNAWKCYAVIVRKTTGWQKEI
                     DYISVSQFKNLTGIKTDVTVADALKELVELNLIASVKRHGQVTGYRINMPEPSPENGG
                     TPPPENGGTATPKNWGSTKHTTKPTNTKHSISASAAADAPLSAEPPETAPAAKAKKTG
                     RHETELSLLADYGITGQVAADFLQVRKAKRQPLTETAMRLIAADAEKCGMTALQAAEY
                     AIASGWGSFRADWLQNKTFGRSGNRGGPTHNQTAAVPDAGSYGDMPTTDF"
     gene            6724..>7498
                     /locus_tag="WNA25_001864"
     CDS             6724..>7498
                     /locus_tag="WNA25_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFA9824558.1"
                     /translation="MALRNASDFLGAYGGGVRVERRQCAEHGGYAAKSVLRGVWTGCP
                     ACRKLEAADEMAAYAETLRRGAMRDALEKRIGRSGIAPRFRNCRIENYAVSDSIPGMA
                     RAKAAAAEYAANFADVLQTGRSMIFSGRRGTGKNHLACGIAREVIAAGKSALVITVGD
                     MLRTVKDSFGGGGGEAGAVGVFVKPDLLVLDEFGAGSLSETDGRILFSVVNARYERLM
                     PMLVLTNLTAEAFRENTDARIRDRLRDGGGKLIPFDWESY"
BASE COUNT         1756 a         1800 c         2100 g         1842 t
ORIGIN      
        1 ccctcgcgcc gacccggttt attttgctga ttaattcgtt catcccgttt ccttcaagtt
       61 gttgtttgtt tcgatgggtg tatattatct aatagataat cttaggtcaa taagttagat
      121 aagtttttta gataattttt attatctatt tgaatttgaa aagaattaaa ttcttctgtt
      181 tgctcgtatc ttttaggcaa caaaaaaacc gcccgatatt tcgggcggcg tattaaaatc
      241 agatgaaatt aaggaggctt attatggaaa tgcaattgga caaacagaca ttgacagtgg
      301 ttgtcagaaa cagcgagccg gtggaattgt cggtatttgc gcagtctatg atgagccttg
      361 ccgatgatta cgaatccatg tgcggcagcg cgggcagtca tgcgcggctg tatattaagg
      421 aaatcaggca gggcagcatt attgccgagc ttgcacccct cttgcctttg gcgggtacgc
      481 tttttgaagg cgcggggcag atattggcgt acgccaagaa ttttattgag attaccgatt
      541 ggctgatggg taaaggcaaa gaacccgcag gggtaacgga cagccaaatc cgcaatattg
      601 caaatatcat gcagcctgcc gcatcggata aaaacgggtc gattgaaatc aacgtgaacg
      661 acaacaacgg ctccgtcgtc aataatatta cctacaacta tttcgcggca aacacggtac
      721 aaaatcaggc aaggcggata ctgggcgaac gggcggaagc gtccgaatca ggggattacg
      781 ggcagatggt gatgtatttc gtacaagccg cgccgacaaa ggaaaccaat caggcggtta
      841 ttgaagggat atacagccgt ccggtcaaaa tcctgattcc cgaacacatc aagcgcgaaa
      901 tgtttgcaga gccttatcca tttgaaaaat attatattgt tgacgtaagc gtacagacgg
      961 cgcgcggcaa gcccaggctc tacaaagtta ccgggtatca cggggtagtc gacggggacg
     1021 attgaaaacc gccttttcgg gcggctttgt cggttttgtg ttgttttcag gttcggcggg
     1081 gcgtgaaaaa agcccgcgtg tgcgggcggg ttgaacgggc tggctatgcc tgattcatgg
     1141 tgagcagggc ggctttggct aaaaaaccgc tacgggtttc atgatttgcg gatgtatatt
     1201 catccactct atcaagaagg tattgcggcc agctgacgtt gaatcggatt tgctgccgac
     1261 tgatttttgc agggtcgatt tcaatcatta cccatgttgc cccatgataa tcaggttctt
     1321 ggctcaaatc ggcaatgctg cttacggcaa ggtttttgaa ccctccgtcc tcaatcatac
     1381 cgtcaatatg catataggcg gcagaacggg cgttggcgac ggcttcttca acggtgtcgc
     1441 cgcaagagaa acagccggga aggtcgggga tggttacgcc atatgccgaa tgttcgtctt
     1501 tgtgcagggc ggcagggata aacatttgat ttcctttaat tatgttgttc aggtttatgg
     1561 gcgcgtgaga taacccgtcg attgtttgat tagaaggggg gcggcgggaa accccgctgc
     1621 ctgccgttac ttcaaaccgg cttgcttata gatatttttt acagtacctg tcggcaagtc
     1681 ttttttcggg tgcggcacgg ttacacggcc tttttttgtt gggtgcttat attgcgaatg
     1741 gctcccggat tgtgcaactt tataccaacc gtcttgtttg agtagggcaa taacgtctag
     1801 gctattcaat tatcactcct aatcttaatg catggcacgc ctgccaatgt gcgtaataat
     1861 acacactaat ttttattttg tcaattttta tgtgtatttg tgtgtaaaaa tatctttcgg
     1921 cagtagttgc agggaagggt gtaaaaagcc cgcataacgc gggcggtctt gagggctgtc
     1981 agccgcaggc aaagtttctg atgattttgc cttcgcttcg gttatcggca agccgtatgc
     2041 cctgttcctc caggtcgccc aatacgtcaa gcaatgcctt ttcttgtgcc ttgtcgggac
     2101 gctccggcgg ttcgtaggca aacaatattt cagagcggtc gatgtatccg ccgcgccgca
     2161 accgttttat gccggcaatc cacttatcag acttgtaata gatgtcggca gggtctttct
     2221 gtccgaaata gataggcttg atgattttcc gtatttcgcc gttttccgcc ttttgcacca
     2281 aggggatgga ggcgtgaaag cctgacgggt cgccgattgt gctttctttg aaggggtatg
     2341 ccgtctgaag cggctttaac attgcctgta tttgtcgggt aagttctgct tcgggttggc
     2401 ttttagcaaa gctgcgggcg acaaaataat caaacaggcg gttcagttcc tgccccctgt
     2461 cgggggcgag ggttacgccc ggttgggcgg ccataatcag ggcttcgcgc gggcgggtca
     2521 aatggtcgag catggcgcgt atctgatcgg gcgcggagta tgccgccaat ttcccaatcc
     2581 gctgtaattc ttctgcaaag gcacgggtcg ccgctttata gacggacgaa tcgaagcggc
     2641 ggaaaaaacg gctcaatctg ctgtaacgct gttcgatttt aaagtcgaag tagccgcttt
     2701 tgggatgggt tatgatgatg ccgatgttgg caaattcgcg cgtttggaca tagggcataa
     2761 accgtatgac ggcaaaacgc atggcatatt ggttcatgag atgctccaaa gtgttccgtt
     2821 atcaatgcgg cggacggttt cgtcggtgcg gctgcggtta taggcggcgg gcaggtcgcg
     2881 ctcttcgttt gcccatgccc actccggcgg cagattatca cacgcttttc tgtaggctgg
     2941 caatgcgctt ttcagccata attccatctc ttcccgaagc acccaatctt ccaaaatctg
     3001 tctaaatgcc gatgagaaaa tatgattttg cagaaagtta tttggattga agccggtgtc
     3061 gaaggcgcag ttatgatcaa taacaatcaa ggaattgttg caatttctat acaaaaggtt
     3121 agggttgccg attgttctgt cttcattacg gacaaaccaa tcgaaagcgg cgatttgccg
     3181 ctgcatgact gtatcgattc gcgggatgtc cgcaggctcc agtaacgcac agcctttttg
     3241 cgccagtgag ccgaaacaga tgcctttgcc gatttctttc atctttgtgg gcaattcttc
     3301 gtacagctct tctccgactt ccaacagctc aaacggagca acgggcaagc ccaaagcctg
     3361 cgccatgttt ccgcctatcc attcgtttat ttggcttgca cgggtggcgt gcagcccttt
     3421 aacgaaatat tccagaccat tctccgccat acagatgaac ggcgaggtaa caccttgctc
     3481 cgcacgatcc attattgtct gtatctgcaa catttctttt ctttatccta tgtttcaaca
     3541 cacaggacga cacataaagc accgccctat gtgttgccct gattcggaag gggttacgcc
     3601 cctcccaaac agagtctgat cctgccgccc taaagggcgg ggtttcaacc gaaaaggaaa
     3661 tacgatgaaa tttacacttt gtgtaaatct gtaaatccga ctttttgaga tttcccccta
     3721 gaggaatttg tcaatccagc acgctccacc aaaataccct accaataacg gtcaggctgt
     3781 ctaaagaggc ggtttcgtct ttgtattccg ggttgtaact cttgatacgg acttgattat
     3841 caggcagctt ttgcaagatt tttgtacgta acagcccact gtggttgatg gcatagattt
     3901 tgccgtcttt aatcatttta tcggcagtat taatacccag tgtagccccg tcggggaata
     3961 ccggctccat actgtctccg tcggcagata cgcagacaac atcgtcaggg cttatgccgt
     4021 gccgtcttaa ggttgatttt gagaaacgca gtttatagcc gttgtagtct tcaatttcat
     4081 ctgaaaagcc gttacccgcc gacaagcaaa cgtctttata aaatggaact tcgcaatcat
     4141 catcggataa cggggttttg ctatcccaag cgtcaacagt gccgataacg gtggcgtttg
     4201 atttgatgga atcaataggt atattcggct cgtcttttcc atccacccat ccgcgcggta
     4261 atccaagcgc ggtctcaatt tgggcggcta cgccgtcccc tatatttctg tatccgttaa
     4321 gccattgatt tatctgtgca ggagctttgt taattgcccg agaaaactca gcttgattgc
     4381 cgttaaacct gtcggctatc agatttttaa ttctatctac ccggttcatt tcatcacccc
     4441 ttcaaataga tataaatata aggcatagct actgcgcgag cttcagggaa atcacgccgt
     4501 agtatctgcc gatgatgcgg aagcggtcgt tttcgtcgat ggggaagctt tggtacagcg
     4561 ggttgtcgga gagggcgagc aggccggcgg gggttttttg caggcgtttg gtgtagaggc
     4621 ctccggcgta ggcgaagaag tagatgccgt cgcctgcgaa ggtgtcggct ttggtgtcga
     4681 taaggacggc ggatgtttcg gggatggtgg gctccatgct gtcgccgcgc gtgccgacga
     4741 ttttgaggcc ggaggtgtcg cggccggaga agaggcggcg cacggcgtcg aggggaaatt
     4801 ccacgctgct gatgggggac ggccagtcgt cggcttcgat gccgccgccg cacgcgcccg
     4861 ccatgtcgag atggtctatg cggatggttt cggtgtcttc gggagggagc ggcagggttt
     4921 ctccgtgatt catatccaac cagccctccg ccttgttcat ggctgactct aattttctcg
     4981 ccattgcccc gcctatgttt ttcggcttcc cgttttgtat cgctgtctga ttgatgattt
     5041 gatacagata gctaggttgg gcatatcccg cacgttttgc caattcggca acgcttccgg
     5101 cttcctctac taaatttttc atattctgca aacggatttc tgcaagaaaa ttcatgtttc
     5161 aactcctatt gttatcgacg agataacaat ataaccaaaa attctctgtt cacaaagttg
     5221 tcaaaagata acataataga tacaatgcaa ttatctaacg gataattaag cgtttgtatg
     5281 aatctgaact cttatctaaa cagcaagcct cggggcgaga tgacgcgcct tgctgctgca
     5341 atcaatgaaa aattatcgaa tttatccagt atgagacacg gaaagaagac tgtgcctccg
     5401 tacaagtgcc gccgaattgt cgaagctaca aacggggaag ttacacttaa agacctgcgc
     5461 ccggacgact ggcacgaaat ttggcctgag ttgaaggagg attgagatgg agattgtgcc
     5521 ggtgaagtgg gaggacgctt tcggttgtcc cgaaggctgg cagatgctgg acggggtggg
     5581 gcgggaaacc agccttgtgc agtcggtcgg ctttgtcgcg gcggaaacgg aaacgacgct
     5641 gacgattgtg ccgcatgtcg gcggcctgaa tcgcgaaaga ggtcggctgc tcgaaagagt
     5701 ttattaataa aatcggtaac ggcgagcgaa aaaacccacg ttatcaaatt gttgattctt
     5761 taaggagttt atacaggaag aatcaaaacc aacccaaata aaaagcccgt cggggatgac
     5821 gggcagccgg ttacgcatta ctcgattgtt gaaaacgggg tggaagtgtg ggcgcaagac
     5881 caatgggaat ggaaggacgg taaatgaaat atattccaaa ttcgtttcag atagcaaacg
     5941 cggtagtgga cgatttcctc tgccgaatga gcggcaacgc gtggaaatgc tacgccgtca
     6001 tcgtgcgcaa aacgaccggc tggcaaaagg aaattgacta catctctgtt tcccaattta
     6061 aaaacctgac cggaatcaaa acagacgtaa cagttgccga cgcgctgaaa gagcttgtgg
     6121 aattgaacct gattgcctcc gtcaaacggc acggtcaggt aaccggctac cgcatcaata
     6181 tgcccgaacc gtcccccgaa aatgggggta caccaccccc ggaaaatggg ggtactgcca
     6241 cccccaaaaa ttgggggtct acaaaacaca ctacaaaacc cactaataca aaacacagta
     6301 ttagcgcatc cgcagcggcg gacgcgcccc tttctgccga acctcccgaa accgcaccgg
     6361 cggcgaaggc gaaaaaaacc ggcaggcacg aaaccgagct ttcgctgctt gccgactacg
     6421 gcatcacggg gcaggtggcg gcggacttcc tgcaagtccg caaggcaaaa cggcagccgc
     6481 tgacggaaac ggcaatgcgc ctgattgccg ccgatgcgga gaaatgcggg atgacggcgc
     6541 tgcaggcggc ggagtacgcc atcgccagcg gctggggcag cttccgcgcc gactggctgc
     6601 aaaacaaaac tttcggcagg tccggaaacc gcggcggccc gacgcacaac caaaccgccg
     6661 ccgtgccgga tgcgggaagc tacggcgata tgccgacgac ggatttttga ggggggttcg
     6721 gatatggctt tgaggaacgc gtctgatttc ttgggggctt acggcggcgg cgtgcgggtc
     6781 gagcggaggc aatgcgcgga acacggcgga tacgcggcga aaagcgtttt gcgcggcgtg
     6841 tggacgggct gcccggcctg ccggaagctg gaggcggcgg acgaaatggc ggcatacgcg
     6901 gaaacgctgc gccgcggggc gatgcgcgac gcgctggaaa aacgcatcgg gcgttcgggc
     6961 atcgccccgc ggttcagaaa ctgccggatt gaaaactacg ccgtcagcga ttcgatcccg
     7021 gggatggcca gggcgaaggc ggccgccgcc gagtatgcgg caaacttcgc cgatgtgttg
     7081 cagacggggc ggagcatgat tttttcgggc aggaggggca cgggcaaaaa ccaccttgcc
     7141 tgcggcatcg cccgcgaagt catcgccgcc ggcaaaagcg cgctggtcat cacggtgggc
     7201 gatatgctgc ggacggtcaa ggacagtttc ggcggcggcg gcggcgaggc gggggcggtc
     7261 ggggttttcg tgaagcccga tttgctggtg ctggacgagt tcggcgcggg cagtctgtcg
     7321 gaaacggacg ggcggatttt gttttccgtc gtcaacgccc ggtacgagcg gctgatgccg
     7381 atgctggtgc tgaccaacct gacggcggaa gccttccgcg aaaacaccga cgcgcggatc
     7441 agggacaggc tgcgggacgg cggcggcaag ctgattccgt tcgactggga gagctacc
//
LOCUS       DAWTQS010000079         7202 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.079, whole genome shotgun sequence.
ACCESSION   DAWTQS010000079 DAWTQS010000000
VERSION     DAWTQS010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7202
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.079"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            79..2241
                     /locus_tag="WNA25_001865"
     CDS             79..2241
                     /locus_tag="WNA25_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFA9824559.1"
                     /translation="MNAPFFRLSLLSLTLAAGFAHAAENNANVALDTVTVKGDRQGSK
                     IRTNIVTLQQKDESTATDMRELLKEEPSIDFGGGNGTSQFLTLRGMGQNSVDIKVDNA
                     YSDSQILYHQGRFIVDPALVKVVSVQKGAGSASAGIGATNGAIIAKTVDAQDLLKGLD
                     KNWGVRLNSGFAGNNGVSYGASVFGKEGNFDGLFSYNRNDEKDYEAGKGFRNDNGGKT
                     VPYSALDKRSYLAKIGTTFGDGDHRIVLSHMKDQHRGIRTVREEFAVSEKNSRITIKR
                     QAPSYRETTQSNTNLAYTGKDLGFVEKLDANAYVLEKKRYSADDKDNGYAGNVKGPNH
                     TRIATRGMNFNFDSRLAEQTLLKYGINYRHQEIKPQAFLNSEFSIPIKEKKNGQEVDK
                     PMEQQKKDRADEAIVRSYRLTNPTKTDTGAYIEAIHEIDGFTLTGGLRYDRFKVKTHD
                     GKTVSSSSLNPSFGVIWQPREHWSFSASHNYAGRSPRLYDALQTHGKRGIISIADGTK
                     AERARNTEIGFNYNDGTFAANGSYFRQTIKDALANPQNRHDSVAVREAVNAGYIKNHG
                     YELGASYRTGGLTAKVGVSRSKPRFYDTHPKKLLSANPEFGAQTGRTWTASLAYRFKN
                     PNLEIGWRGRYVQKATGSILAAGQKDRDGKLENVVRQGFGVNDVFANWKPLGKDTLNV
                     NLSVNNVFDKFYYPHSQRWTNTLPGVGRDVRLGVNYKF"
     gene            complement(2401..2463)
                     /locus_tag="WNA25_001866"
                     /pseudo
     CDS             complement(2401..2463)
                     /locus_tag="WNA25_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2547..3416
                     /locus_tag="WNA25_001867"
     CDS             2547..3416
                     /locus_tag="WNA25_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFA9824560.1"
                     /translation="MLRLTTLTLCTVLALGACSPQNSDSAPQAKEQAVSAAQSESASV
                     TVKTARGDVQIPQNPERIAVYDLGMLDTLSKLGVKTGLSVDKNRLPYLEEYFKTTKPA
                     GTLFEPDYETLNAYKPQLIIIGSRAAKAFDKLNEIAPTIEMTADTANLKESAKERIDA
                     LAQIFGKQAEADKLKAEIDASFEAAKTAAQGKGKGKGLVVLVNGGKMSAFGPSSRLGG
                     WLHKDIGVPAVDESIKEGSHGQPISFEYLKEKNPDWLFVLDRSAAIGEEGQAAKDVLN
                     NPLVAETTAWKKG"
     gene            3536..3727
                     /locus_tag="WNA25_001868"
     CDS             3536..3727
                     /locus_tag="WNA25_001868"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824561.1"
                     /translation="MPSETRMQTASCVSDGIARYGRYRGNGGKRFDWNLPHPLPVRRF
                     QTRLSAPTVRTALFESPTP"
     gene            3746..4714
                     /locus_tag="WNA25_001869"
     CDS             3746..4714
                     /locus_tag="WNA25_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFA9824562.1"
                     /translation="MTAKPFSLNLANLLLPAVLFAVSLSVGVADFRWSDVFSLSDSQQ
                     VMFISRLPRTFAIVLTGASMAVAGMIMQILMRNRFVEPSMAGAGQSAALGLLLMSLLL
                     PAAPLPVKMSVAAVAALIGMLVFMLLIRRLPPTAQLMVPLVGIIFGGVVEAVATFIAY
                     KNEMLQMLGVWQQGDFSSVLLGRYELLWITGGLAVFAYLIADRLTILGLGETVSVNLG
                     LNRTAVLWSGLIIVALITSLVIVTVGNIPFIGLVVPNIVSRLTGDRLRQSLPAVALLG
                     ASMVLLCDILGRMIVFPFEIPVSTVFGVLGTALFLWLLLRKPAHAV"
     gene            4704..5678
                     /locus_tag="WNA25_001870"
     CDS             4704..5678
                     /locus_tag="WNA25_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFA9824563.1"
                     /translation="MPSEKNIGFMAGSSRPLRVAFALLLVSCILFMTVNVKGDWDFVL
                     HLRLTKLAALLMVAYAVGVSTQLFQTLTNNPILTPSILGFDSLYVFLQTLLVFTFGGV
                     GYTSLPLTGKFGFELVVMMGGSLLLFYTLIRQGGRDLPRMILIGVIFGILFRSLSSLL
                     SRMIDPEEFTAAQANMFAGFNTVRSELLGIGALVLLVSAAVVWRERYRSDVHLLGRDQ
                     AVNLGISYTRNTLWILLWIAALVATATAVVGPVSFFGLLAASLANHFSPSVRHSVRLP
                     MTVCVGGILLVGGQTVFEHFLGMKAVLSVVVEFAGGLVFLYLVLKHKK"
     gene            5723..6349
                     /locus_tag="WNA25_001871"
     CDS             5723..6349
                     /locus_tag="WNA25_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824564.1"
                     /translation="MTQERLPEFFDRAPTLTVQDPLAAFLGAAENGILTYRYADAVRL
                     CGHSCPTVAGAYLMVIKGLKALYGEELPERGGIEAAMQGTRDEGTVGVTASVVQLLTG
                     AAPETGFGGIGMQGRFARRNLLSFGAGEINGTLTLRRKDSGKTVAVSLNAALQPFAPQ
                     MRDIMPKAVGGSASAKELERFGQLWQARVKAFLTESADDPQFVIVREL"
     gene            6433..7191
                     /locus_tag="WNA25_001872"
     CDS             6433..7191
                     /locus_tag="WNA25_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFA9824565.1"
                     /translation="MITIRNVSYRIGTHPILDNVSLDIPEGGITALIGPNGAGKSTLF
                     SFMARLRPLESGSIAYRGKNLAGTPTAELARTLSILTQENSIMSRITVRDLLMFGRYP
                     YHQGRPTAECRRIVNGAIEEFHLQDLSDRYLTELSGGQRQRAMIAMVFCQSTDYVLLD
                     EPLNNLDMYHARSLMQILQKLTHEHKRTTVAVLHDINQAAAYADHVVAMKNGKAALTG
                     TPEEVFTVHNIKELFDMDVDVLDYEGKKLIVHHI"
BASE COUNT         1654 a         2166 c         1872 g         1510 t
ORIGIN      
        1 tattattatt ttttcttatc ctgccaaacc ttaacggttt ggcttaactt cccttcatac
       61 actcaaaagg acgaacaaat gaacgccccg tttttccgcc tcagcctgct ctcgctcaca
      121 cttgccgccg gctttgccca cgcggcagaa aataatgcca atgtcgcatt ggataccgtt
      181 accgtaaaag gcgaccgcca aggcagcaaa atccgtacca acatcgttac gcttcaacaa
      241 aaagacgaaa gcaccgcaac cgatatgcgc gaactcttaa aagaagagcc ctccatcgat
      301 ttcggcggcg gcaacggcac gtcccaattc ctgacgctgc gcggtatggg tcagaactct
      361 gtcgacatca aggtggacaa cgcctattcc gacagccaaa tcctttacca ccaaggcaga
      421 tttattgtcg atcccgcttt ggttaaagtc gtttccgtac agaaaggcgc gggttccgcc
      481 tctgccggta tcggcgcgac caacggcgcg atcatcgcca aaaccgtcga tgcccaagac
      541 ctgctcaaag gcttggataa aaactggggc gtgcgcctca acagcggctt tgccggcaac
      601 aacggcgtaa gctacggcgc aagcgtattc ggaaaagagg gcaacttcga cggtttgttc
      661 tcttacaacc gcaacgatga aaaagattac gaagccggca aaggtttccg caatgacaac
      721 ggcggcaaaa ccgtaccgta cagcgcgctg gacaaacgca gctacctcgc caaaatcgga
      781 acaaccttcg gcgacggcga ccaccgcatc gtattgagcc acatgaaaga ccaacaccgg
      841 ggcatccgca ctgtgcgtga agagtttgcc gtcagcgaaa aaaattcacg gataactatt
      901 aaacgccaag ccccatccta ccgcgaaacc actcaatcca acaccaactt ggcgtacacc
      961 ggcaaagatt tgggctttgt cgaaaaactg gatgccaacg cctatgtgtt ggaaaagaaa
     1021 cgctattccg ccgatgacaa agataacggc tacgcaggca atgtaaaagg ccccaaccat
     1081 acccgaatcg ccactcgggg catgaacttc aacttcgaca gccgccttgc cgaacaaacc
     1141 ctgttgaaat acggtatcaa ctaccgccat caggaaatca aaccgcaagc gtttctgaat
     1201 tcggaattct ccatcccgat aaaagaaaag aaaaacggtc aagaagtcga taaaccgatg
     1261 gaacaacaaa agaaagaccg tgcagatgaa gccattgtcc gttcctaccg cctgaccaac
     1321 ccgaccaaaa ccgataccgg cgcgtatatc gaagccattc acgagattga cggctttacc
     1381 ctgaccggcg ggctgcgtta cgaccgcttc aaggtaaaaa cccacgacgg caaaaccgtt
     1441 tcaagcagca gccttaaccc gagtttcggc gtgatttggc agccgcgcga acactggagc
     1501 ttcagcgcga gccacaacta cgccggccgc agcccgcgcc tgtatgacgc gctgcaaacc
     1561 cacggcaaac gcggcatcat ctcgattgcc gacggcacca aagccgaacg cgcgcgcaat
     1621 accgaaatcg gcttcaacta caacgacggc acgtttgccg caaacggcag ctacttccgg
     1681 cagaccatca aagacgcgct tgccaatccg caaaaccgcc acgactccgt cgccgtccgc
     1741 gaagccgtca acgccggcta catcaaaaac cacggttacg aattgggcgc gtcctaccgc
     1801 accggcggtc tgaccgccaa agtcggcgta agccgcagca aaccgcgctt ttacgatacc
     1861 catcctaaaa aactgttgag cgcgaacccc gagtttggcg cacaaaccgg ccgcacttgg
     1921 acggcctccc ttgcctaccg cttcaaaaac ccgaatctgg aaatcggctg gcgcggacgc
     1981 tatgttcaaa aagctacggg ttcgatattg gcggcaggtc aaaaagaccg cgacggcaaa
     2041 ttggaaaacg ttgtacgcca aggtttcggt gtgaacgacg tcttcgccaa ctggaaacca
     2101 ttgggcaaag acacgctcaa tgtcaatctt tcggttaaca acgtgttcga caagttctac
     2161 tatccgcaca gccaacgctg gaccaatacc ctgccgggcg tgggacgtga tgtacgcttg
     2221 ggcgtgaact acaagttcta aaacgcacat cccgaaaaaa tgccgtctga aagcctttca
     2281 gacggcatct gtcctgataa tttgatatat agtggattaa caaaaaccgg tacggcgttg
     2341 ccccgccccg gctcaaaggg aacgattctc taaggtgctg aagcaccaag tgaatcggtt
     2401 ccgtactatt tgtactgtct gcggcttcgc cgccttgtcc tgatttaaat ttaatccact
     2461 atagattatc atttatcctt tctaaagccg ttccggtttg tccgaccggc ggctttgccc
     2521 caatatcccc attttggaga caccctatgt tgcgtttgac cacattgacc ctctgcaccg
     2581 tcctcgcctt gggcgcgtgt tcgccgcaaa attccgactc tgccccacaa gccaaagaac
     2641 aggcggtttc cgccgcacaa tccgaaagcg cgtccgttac cgtcaaaacc gcgcgcggcg
     2701 acgttcaaat accgcaaaac cccgaacgca tcgccgttta cgatttgggt atgctcgaca
     2761 ccttgagcaa actgggcgtg aaaaccggtt tgtccgtcga taaaaaccgc ctgccgtatt
     2821 tagaggaata tttcaaaacg accaaacctg ccggaacttt gttcgagccg gattacgaaa
     2881 cgctcaatgc ctacaagccg cagctcatca tcatcggcag ccgcgccgcc aaggcgtttg
     2941 acaaattgaa cgaaatcgcg ccgaccatcg agatgaccgc cgacaccgcc aacctcaaag
     3001 aaagtgccaa agagcgcatc gacgcgctgg cgcaaatctt cggcaaacag gcggaagccg
     3061 acaagctgaa ggcggaaatc gacgcgtctt ttgaagcggc gaaaaccgcc gcgcaaggca
     3121 aaggcaaagg caaaggtttg gtggttttgg tcaacggcgg caagatgtcg gctttcggcc
     3181 cgtcttcacg actgggcggc tggctgcaca aagacatcgg cgttcccgct gtcgatgaat
     3241 caattaaaga aggcagccac ggccagccta tcagcttcga atacttgaaa gagaaaaatc
     3301 ccgactggct gtttgtcctc gaccgcagcg cggccatcgg cgaagagggt caggcggcga
     3361 aagacgtgtt gaacaatccg ctggttgccg aaacgaccgc atggaaaaaa ggataggtcg
     3421 tttacctcgt tcccgaaact tatttggcag ccggcggcgc gcaagagctg ctgaacgcga
     3481 gcaaacaggt tgcggatgcg ttcaacgccg cgaaataatg aaacggcggc attcgatgcc
     3541 gtctgaaaca cggatgcaaa ccgcttcctg tgtttcagac ggcattgccc gatacgggcg
     3601 ttatcgcggg aatggcggca agcgttttga ttggaacttg ccccatcctc tgcccgtccg
     3661 gaggtttcaa acaaggcttt ccgctccgac ggttcggact gccttgtttg aatctcctac
     3721 gccgtaacgc ttttcccttc tgtttatgac tgccaaacct ttttccctca acctggccaa
     3781 cctcctgctg ccggcggtat tgtttgccgt cagcctgtcg gtcggcgttg ccgatttccg
     3841 ctggtcggat gtgttttcgc tgtccgacag ccaacaggtc atgttcatca gccgcctgcc
     3901 gcgcacgttt gcgattgtgt tgacgggcgc gtcgatggcg gtggcgggga tgattatgca
     3961 gattctgatg cgcaaccgtt ttgtcgagcc ttctatggcg ggtgcgggcc aaagtgcggc
     4021 tttgggtttg cttctgatgt ccctgctgct gcctgccgcg ccgctgccgg tcaaaatgtc
     4081 ggttgccgcc gttgccgcgc tgatcgggat gttggtcttt atgctgctga tccgccgcct
     4141 gccgccgacg gcgcaactga tggtgccgct ggtggggatt attttcggcg gcgtggttga
     4201 ggcggtggcg acgtttatcg cgtataaaaa cgaaatgctg caaatgctcg gcgtgtggca
     4261 gcagggcgat ttttcgagcg tgctgctggg gcggtacgag ctgctttgga ttacgggcgg
     4321 tttggcggtg tttgcctacc tgattgccga ccggctgacg attttggggc tgggcgaaac
     4381 ggtgagcgtg aatttgggtt tgaaccggac ggcggtgttg tggtcgggtt tgattattgt
     4441 ggcactgatt acatcgctgg tcattgtaac ggtcggcaat attccgttta tcgggctggt
     4501 cgtgccgaat atcgtcagcc gcctgacggg cgacaggctg cgccaaagcc tgcctgcggt
     4561 cgccctcttg ggcgcgtcta tggtgctgct gtgcgatatt ttgggacgca tgattgtgtt
     4621 tccgtttgaa attccggtct ctacggtttt tggtgtgttg ggtacggctt tgtttttgtg
     4681 gcttttatta aggaaacctg cccatgccgt ctgaaaaaaa tatcggtttt atggcaggaa
     4741 gcagccgtcc gttgcgggtc gcctttgcgc tgttgctggt ttcctgcatc ctgtttatga
     4801 cggtcaacgt caaaggcgat tgggactttg tcttgcacct gcgcctgacc aagcttgccg
     4861 cgctgctgat ggtcgcctat gcggtcggcg tgtccactca actcttccaa acgctgacca
     4921 acaacccgat tctgacccct tcgattttgg gtttcgattc gctgtatgtg tttttgcaga
     4981 ccttgctggt gtttacgttc ggcggcgtgg gctatacatc cctgccgttg acgggcaaat
     5041 tcggctttga actggttgtt atgatgggcg gctcgctgct gctgttttac acgctcatcc
     5101 gtcagggcgg gcgcgatttg ccgcgcatga ttttaatcgg cgtgattttc gggattttgt
     5161 tccgcagtct gtcgtcgctg ctttcgcgca tgatagaccc cgaagaattt accgccgcgc
     5221 aggcgaatat gtttgccgga ttcaataccg tccgcagcga gcttttgggc ataggcgcgc
     5281 tggtcctgct cgtcagcgcg gcggtcgttt ggcgcgaacg ctaccgctcg gacgtacacc
     5341 ttttggggcg cgaccaagcc gtcaatttgg gcatcagcta cacgcgcaac accttatgga
     5401 tactgctttg gattgccgca ttggtggcga cggcgaccgc cgtggtcggc ccggtaagct
     5461 ttttcgggct tctcgccgcc tcgcttgcca accacttttc cccgtccgtg cgccattccg
     5521 tccgcctgcc gatgacggtt tgcgtcggcg gcatcctctt ggtcggcgga caaaccgtat
     5581 tcgaacactt cttgggcatg aaggcggtat taagcgtggt ggtcgaattt gcgggcggac
     5641 tcgttttcct ctatctcgtt ttaaaacaca aaaaatgacg gatgccgtct gaacggcagc
     5701 ccgccccgaa aggacaaacc atatgacgca agaacgttta cccgaatttt tcgaccgcgc
     5761 cccgacgctg accgtacaag acccgcttgc cgcattcctc ggcgcggccg aaaacggcat
     5821 cctcacttac cgctacgccg atgccgtgcg cctgtgcgga cattcctgcc cgaccgtcgc
     5881 gggcgcgtac ctgatggtta tcaaaggcct gaaagcactt tacggcgaag agctgcccga
     5941 acgcggcggc atcgaagccg ccatgcaggg aacgcgcgac gaaggcacgg tcggcgtaac
     6001 cgcgtccgtc gtccaactcc ttaccggcgc agcccccgaa accggcttcg gaggcatcgg
     6061 aatgcaggga cgcttcgccc gccgcaacct cttatccttt ggtgcaggcg aaatcaacgg
     6121 cacactgacc ctgcgccgca aagacagcgg caaaaccgtc gccgtcagcc tcaacgccgc
     6181 cctgcaaccc tttgcacctc aaatgcgcga catcatgccc aaagccgtcg gcggcagcgc
     6241 aagcgcaaaa gaactcgaac gcttcggaca actttggcag gcacgcgtta aagcattttt
     6301 gaccgaatcg gcggacgacc cgcagttcgt catcgtccgc gaactgtgaa cgttcagacg
     6361 gcattccgaa tttcaaatgc cgtccgaacc cccgccaaac aacaacaaac ctacgcccga
     6421 caagcatccg ccatgattac catccgcaac gtcagctacc gcatcggcac acaccccatc
     6481 ctcgacaacg tcagcctcga catccccgaa ggcggcatta ccgcgctaat cggccccaac
     6541 ggtgcgggca aatccaccct gttttccttt atggcgcggc tgcgcccgct tgaaagcggc
     6601 agcatcgcct accgaggcaa aaaccttgcc ggcaccccca ccgccgaact cgcccgcacg
     6661 ctctccatcc tcacccaaga aaacagcatc atgagccgca tcaccgtgcg cgacctgctg
     6721 atgttcggcc gttaccccta ccatcaaggc agaccgactg ccgaatgccg ccgtatcgtt
     6781 aacggtgcaa tcgaagaatt ccacctgcag gacctctccg accgctacct gaccgaactc
     6841 tccggcggac aacgccaacg cgccatgatt gcgatggtgt tctgccaaag caccgactac
     6901 gtccttttgg acgaaccgct gaacaacctc gatatgtatc acgcccgctc gctcatgcag
     6961 attctgcaaa agctcacgca cgaacacaaa cgcaccaccg tcgccgtatt gcacgacatc
     7021 aaccaggccg ccgcctacgc cgaccacgtc gtcgccatga aaaacggcaa agccgccctg
     7081 accggcacac ccgaagaagt gttcaccgta cacaacatca aagaactgtt cgacatggac
     7141 gtggacgtac tcgactacga aggcaaaaaa ctcatcgtcc accacatctg aagctcaaaa
     7201 ag
//
LOCUS       DAWTQS010000080         7189 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.080, whole genome shotgun sequence.
ACCESSION   DAWTQS010000080 DAWTQS010000000
VERSION     DAWTQS010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7189
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.080"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            89..493
                     /locus_tag="WNA25_001873"
     CDS             89..493
                     /locus_tag="WNA25_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HFA9824566.1"
                     /translation="MTAQPCPICTAQDEDILLQTPKLRVIAVHNDSGSPAFCRVIWHG
                     HIAEITDLSAAERGELMEMVYKVEAAMRQVFRPAKINLASLGNVVPHLHWHIIARFEN
                     DATFPAPIWANPVRKHGMTLPQNWTEQLKKLL"
     gene            533..1714
                     /locus_tag="WNA25_001874"
     CDS             533..1714
                     /locus_tag="WNA25_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HFA9824567.1"
                     /translation="MTEPTSRRRFLKTCTAAGAGLLQACGTSATSVPPLPSSHSVVKA
                     RTVPLQTPRRQSSDGNLLRVVASSGFAEDTNRVNTALTRLYNAGFTVTNQQAGSRRFQ
                     RFAGTDAQRAADFQEVASGRVATPKVLMGLRGGYGAARILPHIDFASLGARMREHGTL
                     FFGFSDVCAVQLALLAKGNMMSFAGPMAYSDFGKPAPGAFTMDAFIKGTTQNRLTVDV
                     PYIQRADVETEGTLWGGNLSVLASLAGTPYMPDIDGGILFLEDVGEQPYRIERMLNTL
                     YLSGILGKQRAIVFGDFRMEKIRDLYDSSYDFSAVAKHISRTAKIPVLTGFPFGHIAD
                     KITFPLGAHTRIRMNGNGGYSVAFEGYPTLDASALTLDTLLPPPDLPIFPESGVADIS
                     E"
     gene            complement(1816..4071)
                     /locus_tag="WNA25_001875"
     CDS             complement(1816..4071)
                     /locus_tag="WNA25_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HFA9824568.1"
                     /translation="MGQYKKLWYLLFAVLAVCFTILGYMGSEVYKKAPPYPEQVVSAS
                     GKVLMTKDDILAGQSAWQTTGGMEVGSILGHGAYQAPDWTADWLHRELSAWLDLTAQQ
                     AYGKKFDEVSPEEQAVLKTRLADEYRNQSRIKEDGSVVISDTRVKAIESILPYYHGVY
                     GDDPKLQTTREHFAMKNNTLPSQEAREKLFDFFFWTSWSASTNRPGEVFTYTNNWPHE
                     PLINNVPTTENYMWSFTSVVLLLMGIGLLMWGYSFLTKHEEVEVPSEDPISKIQLTPS
                     QKALGKYVFLTVALFVVQVLLGGLTAHYTVEGQGFYGIDEALGFEMSDWFPYALTRTW
                     HIQSAIFWIATGFLTAGLFLAPIVNGGKDPKFQRAGVNFLYIALFIVVGGSYAGNFFA
                     LTHILPPEFNFWFGHQGYEYLDLGRFWQLLLMVGLLLWLFLMLRCTVSAFKEKGVDKN
                     LLAIFVASMVGVGVFYAPGLFYGEKSPIAVMEYWRWWVVHLWVEGFFEVFATAAFAFV
                     FYNMGFVRRSTATASTLAAAAIFMLGGVPGTLHHLYFSGSTSASMAIGACFSALEVVP
                     LVLLGREAYEHWSYQHLSDWAKRLRWPLMCFVAVAFWNMIGAGVFGFLINPPISLFYI
                     QGLNTSAVHAHAALFGVYGFLALGFVLLVARYLKPNARFDDKLMTWGFWLLNGGLVGM
                     IAISLLPVGVIQAYASITHGLWYARSEEFLQMEILDTLRWVRTAPDLIFIGGAICVAI
                     QATKIVFGRDK"
     gene            4444..5622
                     /gene="nirK"
                     /locus_tag="WNA25_001876"
     CDS             4444..5622
                     /gene="nirK"
                     /locus_tag="WNA25_001876"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase, copper-containing"
                     /protein_id="HFA9824569.1"
                     /translation="MKRQALAAMIASLFALAACGGEQAAQAPAETPAASAEAASSAAQ
                     ATAETPAGELPVIDAVTTHAPEVPPAIDRDYPAKVRVKMETVEKTMKMDDGVEYRYWT
                     FDGDVPGRMIRVREGDTVEVEFSNNPSSTVPHNVDFHAATGQGGGAAATFTAPGRTST
                     FSFKALQPGLYIYHCAVAPVGMHIANGMYGLILVEPKEGLPKVDKEFYIVQGDFYTKG
                     KKGAQGLQPFDMDKAVAEQPEYVVFNGHVGAIAGDNALKAKAGETVRMYVGNGGPNLV
                     SSFHVIGEIFDKVYVEGGKLINENVQSTIVPAGGSAIVEFKVDIPGSYTLVDHSIFRA
                     FNKGALGQLKVEGAENPEIMTQKLSDTAYAGSGAASAPAASAPAASAPAASASEKSVY
                     "
     gene            5800..6555
                     /locus_tag="WNA25_001877"
     CDS             5800..6555
                     /locus_tag="WNA25_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HFA9824570.1"
                     /translation="MKYVRLFFLGTALAGTQAAAAEMVQIEGGSYRPLYLKKDTGLIK
                     VKPFKLDKYPVTNAEFAEFVNSHPQWQKGRIGSKQAEPAYLKHWMKNGSRSYAPKAGE
                     LKQPVTNISWFAANAYCAAQGKRLPTIDEWEFAGLASATQKNGSNEPGYNRTILDWYA
                     DGGRKGLHDVGKDRPNYWGVYDMHGLIWEWTEDFNSSLLSSGNANAQMFCSGASVGAS
                     DSSNYAAFLRYGIRTSLQSKYVLHNLGFRCASR"
     gene            6741..>7189
                     /locus_tag="WNA25_001878"
     CDS             6741..>7189
                     /locus_tag="WNA25_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HFA9824571.1"
                     /translation="MSDFQIIFEYCRCSKVRIPARAQVSEAILLAEGQKSAVTEYYLN
                     NGEWPENNGAAGVASASDIKGKYVESVTVANGVVTAKMKPSGVNKEIQGKKLSLWAKR
                     ENGSVKWFCGQPVTRTEANAKAGTDDVAKDDAAGKKIDTKHLPSTCR"
BASE COUNT         1818 a         2038 c         1808 g         1525 t
ORIGIN      
        1 tggtacaatt aacggcaatg cggacagaca aattaaacta tcagaaattg ccctaccgtt
       61 ttttaaacac tttcaggaat aaggaaaaat gaccgcccaa ccctgcccca tctgcacggc
      121 acaagatgaa gatattttgc tgcaaacccc caagctgcgc gtcatcgccg tccataacga
      181 cagcggttcg ccggcattct gccgcgtcat ttggcacgga cacattgccg aaattaccga
      241 cctttcggca gcggaacgcg gcgaattgat ggaaatggtg tacaaagtcg aagccgctat
      301 gcgccaagtg ttccggccgg caaaaatcaa cctcgccagc ttgggcaatg tcgtgccgca
      361 cctgcattgg catattatcg cccgctttga aaacgatgcg actttccctg cgccgatttg
      421 ggcaaacccc gtccgaaaac acggtatgac cctgccgcaa aattggacgg aacagcttaa
      481 aaagctgctt taaacccgcc gatgccgtct gaaaccgtat gaaagggaaa ttatgaccga
      541 accgacctcc cgccgccgtt ttctgaaaac ctgcaccgcc gccggcgcgg ggctgcttca
      601 ggcttgcggc acatccgcca catccgttcc gccccttccc tcttcccatt ccgttgtgaa
      661 agcccgaacc gtgcctctcc aaacgccacg ccgtcaaagt tcggacggca accttctgcg
      721 cgttgtcgct tcgtcaggat ttgccgaaga cactaaccgc gtcaacacag ccttaacccg
      781 cctttacaat gccggtttta ccgtaaccaa ccaacaggcg ggcagccgcc gtttccaacg
      841 gtttgccggc acggacgcgc aacgcgcggc agatttccaa gaggtcgcct ccggccgcgt
      901 cgccacgcct aaagtgctga tgggcttgcg cggcggttac ggtgcggcgc ggattctgcc
      961 gcatatcgat tttgcctcgc tcggcgcaag gatgcgcgaa cacggcacgc tgtttttcgg
     1021 attcagcgac gtatgcgccg tccaactggc attgttggca aaaggcaata tgatgagttt
     1081 tgccggcccg atggcttata gcgattttgg caaacctgcc cccggtgcgt ttacgatgga
     1141 tgcctttatc aaggggacaa cccaaaaccg cctgaccgtt gatgttcctt atatccaacg
     1201 cgccgatgtc gaaaccgaag gcacgttgtg gggcggcaac ttaagcgtcc tcgcctcgct
     1261 cgccggtacg ccttatatgc ccgacatcga cggcggcatt ttgttcctcg aagatgtcgg
     1321 cgaacagccc taccgcatcg aacgtatgct caatacgctg tatctttcgg gtattttggg
     1381 caaacagcgc gccatcgtgt tcggcgattt ccgtatggaa aaaattcgag atctctatga
     1441 ttcgtcttat gatttttccg ccgttgccaa gcatatttcg cgcacggcga aaatccccgt
     1501 gctgacgggc ttcccgttcg gacacattgc cgacaaaatc actttccctc tgggcgcgca
     1561 cacccgaatc cgtatgaacg gaaacggcgg ctattcggtc gcgtttgaag gctaccccac
     1621 actcgatgcg tccgccctga ctttggatac cctgctccca ccgccggatt tgcctatctt
     1681 ccccgaaagc ggtgttgccg atatttcgga ataaacccgc aaacggacaa atgccgtctg
     1741 aagccttcag acggcatttt cctgtaaaaa aaccgcacct gatggcaggt gcggtttcca
     1801 ttttcaggct aaaacttatt tgtcgcggcc gaatacgatt ttagtggctt ggatggcaac
     1861 gcagattgca ccgccgataa agattaagtc gggggcagtg cgtacccagc gcagggtgtc
     1921 gaggatttcc atttgcagga attcttcgct gcgggcatac cacaaaccgt gcgtgataga
     1981 ggcgtatgcc tgaatcacgc cgacaggcag caggctgatg gcaatcatac cgaccaagcc
     2041 gccgttgagc agccagaagc cccaagtcat cagtttgtcg tcaaaccgcg cgttcggttt
     2101 caagtagcgc gcaaccaaca atacgaagcc caatgccaag aaaccgtaca caccgaacaa
     2161 ggcggcgtgc gcgtgaacgg cggaagtgtt caaaccttgg atatagaaca gggaaatcgg
     2221 cggattgatc agaaagccga atacgccggc accgatcata ttccaaaaag cgactgccac
     2281 gaagcacatc agcggccaac gcaggcgttt cgcccagtcg gacaggtgtt ggtaagacca
     2341 gtgctcgtat gcctcgcggc ccagcaacac cagcggcacg acttccaaag cggagaagca
     2401 ggcgccgatt gccatagagg cggaggtaga gccggagaag tacaggtggt gcagcgtgcc
     2461 cggaacgccg cccaacataa agatggcggc agcggccaaa gtggaggcag tggcggtact
     2521 gcggcggaca aagcccatat tgtagaagac aaaggcaaag gcggcagtgg caaatacttc
     2581 aaagaagcct tccacccaca ggtggaccac ccaccaacgc cagtattcca ttacggcaat
     2641 cggggatttt tcgccataga acaggcccgg tgcgtagaac acgcccacgc cgaccataga
     2701 agcgacaaag attgccagca ggtttttgtc cacgcctttt tctttgaagg cggaaaccgt
     2761 gcagcgcaac atcaggaaca gccacaacag cagtccgacc atcaaaagga gttgccagaa
     2821 acgtcccaaa tcgaggtatt cgtaaccttg gtgtccgaac cagaagttaa attcgggggg
     2881 aaggatgtgc gtcaacgcga agaagttgcc cgcgtaagaa ccgccgacca cgatgaagag
     2941 ggcgatatag aggaagttca cgcctgcacg ttggaacttg ggatctttgc cgccgttgac
     3001 aatcggcgcg aggaacaaac ctgccgtcaa aaagccggtt gcaatccaga agatggcgga
     3061 ttggatgtgc caagtacggg tcagggcgta ggggaaccag tcggacattt caaagcccaa
     3121 cgcctcgtca atgccgtaga aaccctgacc ttcgacggtg tagtgcgcgg tcaggccgcc
     3181 cagcaatact tgtaccacaa acagggcgac cgtcaggaag acgtatttgc ccaatgcttt
     3241 ttgcgaaggg gtcagttgga ttttggaaat cgggtcttca gacggcactt ccacttcctc
     3301 gtgtttggtc aggaaggaat aaccccacat caacaaaccg atgcccatca agagcagtac
     3361 gacactggta aacgaccaca tgtagttttc agtagtcggt acattgttga tcaaaggctc
     3421 gtgcggccag ttgttggtgt aggtaaagac ctcgccggga cggttggtcg aagcagacca
     3481 agaagtccag aagaagaagt cgaacagttt ttcacgcgct tcttggcttg gcaatgtgtt
     3541 gtttttcatt gcaaagtgtt cgcgggtggt ttgcaacttg ggatcatcgc cgtaaacgcc
     3601 gtgatagtaa ggcaggatgc tttcgatggc tttcacgcgc gtatcgctga tgacgacgct
     3661 gccgtcttct ttaatacggc tttgattgcg gtattcatcg gccaagcggg ttttcagaac
     3721 ggcttgttct tcaggggaaa cttcatcgaa ttttttgccg taagcctgtt gcgcggtcaa
     3781 atccaaccag gcggacaact cacgatgcag ccagtcggcc gtccagtccg gagcctgata
     3841 tgcgccgtgt cccagaatcg aaccgacttc catgccgccg gtagtctgcc acgcagactg
     3901 acctgccaaa atatcgtctt tcgtcatcag cactttgccg gatgcggaaa cgacctgttc
     3961 ggggtaaggc ggggctttct tataaacctc gctgcccata tagccaagaa tggtaaagca
     4021 taccgccaga acggcaaaca gcaagtacca cagcttcttg tactgtccca ttttgagagc
     4081 tccttttaat aaatcagttg tttaaaattc acaaaatatg aatgttaaag attgtagcat
     4141 ggtttaccgc gcaaataaac atttgttcaa acaaactcac atatagaaca aatacatata
     4201 tgataataac tatcattatt ctttactcgg aaattgccct gcctttgcct gctctgccgg
     4261 agcccctagc aaatcagcct attcattgta atttttagta gttataaagt attagaagcg
     4321 tcattttaag ttcatatttt atgaattatt tgacttaaat taaaatgccc gcaatgggac
     4381 aaccgcataa tcacaccaag ttcttaacta atccccctac ttttcttaca aaaggaaaac
     4441 attatgaaac gccaagcatt agccgcaatg attgcttcct tattcgcatt ggccgcctgc
     4501 ggtggcgaac aagctgctca agcccctgcc gaaacccctg ccgcttccgc agaagccgca
     4561 agttccgccg cacaagctac cgccgaaacg cctgcaggcg aactgcccgt catcgatgcg
     4621 gtgaccaccc acgctcccga agtacctccc gcaatcgacc gcgactatcc tgccaaagta
     4681 cgcgtaaaaa tggaaaccgt cgaaaaaacc atgaaaatgg acgacggggt ggaataccgc
     4741 tactggacat ttgacggcga cgttccgggc cgtatgatcc gcgtacgcga aggcgatacg
     4801 gttgaagtcg aattctccaa caatccttct tctaccgttc cgcacaacgt cgacttccac
     4861 gccgcaaccg gtcagggcgg cggtgcagcc gcgaccttta ccgccccggg ccgcacttcc
     4921 acattcagct tcaaagccct gcaaccgggc ctgtacatct accactgcgc cgtcgcgccg
     4981 gtcggtatgc acatcgccaa cggtatgtac ggtctgattt tggtcgagcc taaagaaggc
     5041 ctgccgaaag tggataaaga gttttacatc gtccaaggcg acttctacac caaaggcaaa
     5101 aaaggcgcgc aaggcctgca accgttcgat atggacaaag ccgttgccga acagcctgaa
     5161 tacgtcgtat tcaacggcca cgtaggcgct atcgccggcg acaacgccct gaaagcgaaa
     5221 gcaggcgaaa ccgtgcgtat gtacgtcggt aacggcggcc cgaacttggt gtcttccttc
     5281 cacgtcatcg gcgaaatctt cgacaaagtt tatgttgaag gcggcaaact gattaacgaa
     5341 aacgtacaaa gcaccatcgt gcctgccggc ggttctgcca tcgtcgaatt caaagtcgac
     5401 atcccgggca gctacacttt ggtcgaccac tccatcttcc gcgcattcaa caaaggcgcg
     5461 ttggggcaat tgaaagtaga gggtgcggaa aaccctgaaa tcatgactca aaaattgagt
     5521 gataccgctt acgccggcag cggcgcggct tctgcccctg ctgcttccgc accggctgct
     5581 tctgccccgg cagcctctgc atccgaaaaa agcgtttatt aaatcggata cccgtcatta
     5641 gcgggacaaa ccactgccgc cgtacttcat tacgcacggc agtggttttt taacaaccaa
     5701 tctttccttt cggaagattg atttttcaga cggcattcgc ggctttttgt ttaaaatgcc
     5761 gtctgaaaaa tcaatgctaa ccgcctgtca ggaggcttta tgaagtacgt ccggttattt
     5821 ttcctcggca cggcactcgc cggcactcaa gcggcggctg ccgaaatggt tcaaatcgaa
     5881 ggcggcagct accgcccgct ttatctgaaa aaagataccg gcctgattaa agtcaaaccg
     5941 ttcaaactgg ataaatatcc cgttaccaat gccgagtttg ccgaatttgt caacagccac
     6001 ccccaatggc aaaaaggcag gatcggttcc aaacaggcag aacccgctta cctgaagcat
     6061 tggatgaaaa acggcagccg cagctatgcg ccgaaggcgg gcgaattgaa acagccggtt
     6121 accaatattt cctggtttgc cgccaacgcc tattgcgccg cacaaggcaa acgcctgccg
     6181 accatcgacg aatgggaatt tgccggactt gcttccgcca cgcagaaaaa cggctcaaac
     6241 gaacccggct acaaccgcac tattctcgat tggtatgccg acggcggacg gaaaggcctg
     6301 cacgatgtcg gcaaagaccg cccgaactac tggggtgttt atgatatgca cgggctgatt
     6361 tgggaatgga cggaagattt caacagcagc ctgctttctt ccggcaatgc caacgcgcaa
     6421 atgttttgca gcggcgcatc tgtcggggcg agcgactcgt ccaactatgc cgccttcctc
     6481 cgctacggca tccgcaccag cctgcaatcc aaatatgtcc tgcacaactt gggcttccgc
     6541 tgcgcaagcc gataacccct tcaattatag tggattaaca aaaaccagta cggcgttgcc
     6601 tcgccttgcc gtactggttt ttgttaatcc gctatattcc gccatctcta agatttacag
     6661 cgatacacgg gtaatttaag gaatgcccga accgtcattc ccgccacttt tcgtcattcc
     6721 cgcgaaagcg ggaatctgga atctcggact ttcagataat ctttgaatat tgccgttgtt
     6781 ccaaggtccg gattcccgcc cgcgcgcaag tttccgaagc catccttttg gccgaaggtc
     6841 aaaaatcagc cgttaccgag tattacctga ataacggcga atggccggaa aacaacggcg
     6901 ctgccggcgt ggcatccgcc tccgacatca aaggcaaata tgttgaaagc gttacggtcg
     6961 caaacggcgt cgttaccgcc aaaatgaaac caagcggcgt aaacaaagaa atccaaggca
     7021 aaaaactctc cctgtgggcc aagcgtgaaa acggttcggt aaaatggttc tgcggacagc
     7081 cggttacgcg caccgaagcc aacgccaaag ccggcaccga cgacgtcgcc aaagacgacg
     7141 ccgccggcaa aaaaatcgac accaagcacc tgccgtcaac ctgccgcga
//
LOCUS       DAWTQS010000081         7152 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.081, whole genome shotgun sequence.
ACCESSION   DAWTQS010000081 DAWTQS010000000
VERSION     DAWTQS010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7152
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.081"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            74..223
                     /locus_tag="WNA25_001879"
     CDS             74..223
                     /locus_tag="WNA25_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824572.1"
                     /translation="MNAEGFCEKQTACRPDGRRNAGDRADLKAFEFAGRELANYREML
                     KRYAV"
     gene            253..3300
                     /locus_tag="WNA25_001880"
     CDS             253..3300
                     /locus_tag="WNA25_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFA9824573.1"
                     /translation="MSDLEAKVRITVENHTKQGFDSAAADADKAAEKMRSSGDNAAKG
                     FKAAIDSMHETMRNLHADVKAGFEAAGNQAQQASEKVRAEVGKIGSGLSGLTKLLAGL
                     ATADFAKSVLDTADAMQLINSQVRQVTSSETEYLAVQQQLLDTANRTSASLESTANLY
                     VSTSRALKDYGYTQQEILKFTEATNNAMTIGGVGAQQQAVALMQLSQALGSGVLQGDE
                     FKSISEAAPILLDTIAEYMGKSRDEIKKLGSEGKLTADVIFKAISGTSEKFGEQAAKM
                     PVTMGQALTVFSNNWQSMVSKLLNDSGTMSGIAAVIKLIADNLNLVVPIVAGFAVAVA
                     AAVAPTLALNLALLANPFGIIAVAIGTVVGLIAKFGDEIDVFGGGWSNLSDVIRAVWQ
                     IITETVGEAVGTVKSWFDGLTGRLNEGAGGWPSLFGRVMSVISSAIGAYVNVYINIFA
                     TGWMLIKEAANDMPQFFANLGKAIGNVFISAIERMINKAVGMINSMIDFANKAASMAG
                     ISGIGKLNKVRMGRMDDGGLGERIHDSLTKDRAGAMANAVRERAADIHEADALKGRGG
                     GGHAKTARKKPGANQGGGKGGKSRSGGPGAAKDPMQAWEGEIKARKLAHREMQRETLT
                     HQEWDLAREAEYWRAKLATVDANGKTGVKIREKILTLEDQLSKQSTEAKMNQAAEWEK
                     LDKHKLEMEKDAADQALADGRISQLERLDLEIEFENRRYQIAYDALQERIALAEQDPA
                     YSQAAIGKLKAQMGELGRGHERTQAKNEGKRENQRRKDAPNVMEMLQDGGRNVWQEAQ
                     QQMGQAFTAMLARAQNFRQAMNGFFKSMGQTFIQEMVTKPLTGLMRRMVEESAIYKMI
                     FGTKETLETAAAAKTAATKATETTAVVSSNAVQAASGAAASQAHIPYVGPILAVAAMA
                     AMMSAVMGLMGGGGGSQTTTTTTRIPSAAGGWDIPAGINPLTQLHENEMVLPAEHAQT
                     IREMADQQGGGSTVIINSTGGDFIHKNDLAKLLKQMKRDFKFV"
     gene            complement(3360..3839)
                     /locus_tag="WNA25_001881"
     CDS             complement(3360..3839)
                     /locus_tag="WNA25_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4760 domain-containing protein"
                     /protein_id="HFA9824574.1"
                     /translation="MTDNLIQIATPILTVIGVFVAAYGIMRNTENAKKRATIDMIMAE
                     RNNAALQEAITIVNGLAKTDGCILATYTSDTPDKKKDREAILTVLNQREFVCAGVLGG
                     ALHEKMYKDFEYSMLLRDWDNLSSFIFEIRRIRSAPTAFQEFEAVARKWKKKPLKTK"
     gene            complement(4181..5170)
                     /locus_tag="WNA25_001882"
     CDS             complement(4181..5170)
                     /locus_tag="WNA25_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFA9824575.1"
                     /translation="MATITQRNGKWRVQIRMKGVSRSATFERASDAKAWAARIESQIM
                     DGIQGNAPRNTIFADLIRRYLSEVTPSKRGAREESYRIGRALKTPLAKVRLADLRPQD
                     FADWRDQRLQEVSPTSVGRELTTLSAVCEHAMKEWGLLRENPVRKISKPKKSRARTRR
                     PTEQEIADICAALLYRPNEKPKMAVQRVAVAVLFAIETAMRAGEICGLKWADVNMRRR
                     IAHLPITKNGDSRDVPLSLRAAELIEQLRGIDDTWVFSLDAKSLDVLFRRARDNCGIQ
                     GLHFHDTRREALTRLSKKVPVEVLAKISGHRDLRILLNVYYRPDMADIAKMLD"
     gene            complement(5430..5618)
                     /locus_tag="WNA25_001883"
     CDS             complement(5430..5618)
                     /locus_tag="WNA25_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824576.1"
                     /translation="MADGRKGFFDAIIKINIFFFPVKTPTLGAANPIAGGSTRFRFGA
                     EQMAVLYVPFCVLKHIGR"
     gene            5859..6893
                     /locus_tag="WNA25_001884"
     CDS             5859..6893
                     /locus_tag="WNA25_001884"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFA9824577.1"
                     /translation="MSTSLSYRDAGVGIDAGDQLVEKIKPFAKRTMRPEVLGDLGGFG
                     ALVEIGKKYQNPVLVSGTDGVGTKLKLAFDWDKHDTVGIDLVAMSVNDILVQGAEPLF
                     FLDYFACGKLDVPRATDVIKGIAQGCEESGCALIGGETAEMPGMYPVGEYDLAGFAVG
                     VVEKENVITGRSIGAGDVVLGLASNGAHSNGYSLIRKIIERDNPDLDAEFDNGKTLRE
                     AVIAPTRLYVKPILAALEKFTIKGMAHITGGGITENVPRVLPKNTVAQIDAESWELPK
                     LFQWLQKAGNVETQEMYRTFNCGIGMVVIVAAEDADAVRSFLSGQGETVYRLGCIRER
                     QGNEHQTQVA"
BASE COUNT         1805 a         1724 c         1982 g         1641 t
ORIGIN      
        1 tattccctgc gcgtcaaacc gccttcgtgt aaagcggttg caaaaaagcc ttttattttg
       61 acagggtgca gagatgaacg ccgaaggatt ttgtgaaaaa caaaccgcct gccggccgga
      121 cgggcgccga aatgcgggcg atcgtgccga tttgaaggcc tttgagtttg ccggacggga
      181 attggcgaat tatcgggaaa tgttgaaacg ttatgccgtc tgaaaagacg gcttttttat
      241 ttggggctgt aaatgagcga tttggaagcc aaagtcagga taacggtaga aaaccatacg
      301 aagcagggtt ttgattcggc cgccgccgat gctgataagg cggcggaaaa gatgcgcagc
      361 agcggcgata atgccgcaaa gggctttaaa gctgcaatcg acagtatgca cgagaccatg
      421 cgcaatttgc acgccgatgt gaaagcggga tttgaagcgg cgggcaatca ggcgcaacag
      481 gcgtctgaaa aggttagggc cgaagtgggt aaaatcggct caggcttatc ggggctgacc
      541 aaactgctgg caggattagc aaccgcagac ttcgccaaat cggtgcttga taccgccgat
      601 gcgatgcagt tgataaacag ccaagtccga caagtcacgt cgtctgaaac ggagtatctg
      661 gccgtgcagc agcagcttct cgatacggca aaccgtacca gtgcatcgct tgaatcaacg
      721 gcaaacctgt acgtttccac aagccgcgcg ttgaaagact acggctacac gcagcaggaa
      781 attttaaaat tcaccgaggc aaccaacaac gcgatgacca tcggcggcgt tggcgcgcaa
      841 caacaggccg tcgcgctgat gcagttatcg caggctttgg gcagcggcgt attgcagggc
      901 gatgaattta aatccatttc cgaagccgca cctattttgc tggataccat tgcggaatat
      961 atgggcaaat cccgcgacga aatcaaaaag ctgggcagcg aagggaaatt gacggcggat
     1021 gtgattttca aagccatttc cggcacgtcg gagaagttcg gggagcaggc ggccaaaatg
     1081 cccgtgacga tggggcaggc tttgacggtg ttctcgaaca actggcaaag catggtttca
     1141 aaactgctga acgacagcgg cacaatgtcg gggattgccg ccgttattaa actgattgcg
     1201 gataacctta atttggttgt tccgatcgtc gcaggttttg ccgttgccgt tgccgccgcc
     1261 gttgcaccga cactggcttt gaatcttgca ctgctggcaa acccgttcgg gattattgcc
     1321 gtcgcaatcg gcacggttgt cgggctgatt gcgaaattcg gcgatgaaat cgatgttttc
     1381 ggcggcggct ggtcgaatct ttccgatgtg attcgggcgg tttggcaaat catcacggaa
     1441 accgtcgggg aagcggtggg aaccgtcaaa tcgtggtttg acgggctgac aggccggctg
     1501 aacgagggcg cgggcggctg gccgtcgttg ttcgggcgcg tgatgagcgt catctcaagc
     1561 gcaatcggcg cgtatgtgaa cgtttatatc aacatattcg caaccggctg gatgctgatt
     1621 aaagaagccg caaacgatat gccgcaattc ttcgccaatc tcggcaaggc cataggcaac
     1681 gtgtttattt ccgcgatcga acggatgata aacaaagcgg taggcatgat taacagcatg
     1741 attgactttg ccaacaaagc cgcgtcgatg gccgggatat cgggcattgg aaagctgaac
     1801 aaggttcgga tggggcggat ggacgacggc gggcttggcg agcgtattca tgacagcttg
     1861 acgaaagacc gcgccggcgc aatggcaaat gccgtccgcg aacgggccgc cgatattcac
     1921 gaagccgatg ccctcaaggg acgcggcggc ggaggacacg ccaaaaccgc ccggaaaaag
     1981 ccgggcgcaa atcagggcgg cggcaaaggg ggcaaatccc gttcgggcgg tccgggcgcg
     2041 gccaaagacc cgatgcaggc ttgggaaggg gaaatcaaag cccgaaaact cgcacaccgc
     2101 gaaatgcagc gcgaaacgct cacgcaccaa gaatgggatt tggcgcgtga ggcggaatat
     2161 tggcgggcga agctggcaac ggtggacgcc aacggcaaaa caggcgtaaa aatccgcgaa
     2221 aaaatcctga cgcttgaaga ccaattatcg aagcagtcaa ccgaagccaa aatgaatcag
     2281 gcggccgaat gggagaaatt ggacaagcac aagctggaga tggagaaaga cgcggcagac
     2341 caagccctgg cagacggacg tatttcgcaa ctcgaacgcc tggacttgga aatcgagttt
     2401 gaaaaccgcc gttaccaaat cgcctatgac gcattgcagg aacgcatcgc acttgccgaa
     2461 caagacccgg catacagtca ggcggcaatc ggcaagctca aggcccaaat gggcgaattg
     2521 gggcgggggc acgaacggac gcaggcgaag aacgagggca aacgcgaaaa ccaacgccgc
     2581 aaagacgcgc ccaacgtcat ggaaatgctg caagacggcg gcaggaacgt ttggcaggaa
     2641 gcacagcagc agatggggca ggcgttcacc gccatgctgg cgagggcgca gaacttcagg
     2701 caggcgatga acggtttctt caaaagtatg gggcaaacct ttattcagga aatggttacg
     2761 aaacctttga cgggcttgat gcggcgcatg gttgaggaat cggccattta caagatgatt
     2821 ttcggaacta aggaaacgct ggagacagcg gcggcagcca agacggcggc aactaaggca
     2881 accgagacaa cggcggttgt cagtagtaat gctgttcaag cggcttcagg cgcggcggct
     2941 tcccaggcgc atatcccata tgtcggcccg attcttgccg ttgcggcaat ggcggcaatg
     3001 atgtcggcag tgatggggtt gatgggcggc ggcggcggtt ctcaaacgac cacaaccacg
     3061 acgcgcattc cctcggcggc aggcggctgg gacatcccgg caggcatcaa cccgctgacc
     3121 caactgcacg aaaacgagat ggtcctgcct gccgaacacg cgcaaaccat ccgtgaaatg
     3181 gcggatcagc agggaggcgg cagcaccgtc atcatcaatt cgacaggagg cgatttcatc
     3241 cataaaaatg acttggcgaa gctcttgaag cagatgaaac gggactttaa atttgtctga
     3301 gaaaatgccg tccgaaaggt tcggacggcc tatgttgcgg cgtacgtcaa gctattaagc
     3361 tatttggttt tcagaggctt tttcttccat ttccgggcta cggcttcaaa ttcttgaaag
     3421 gccgtcggcg cgctcctgat acggcgtatt tcaaaaatga agctgcttag gttgtcccag
     3481 tcgcgtaaca gcatggagta ttcgaaatct ttatacattt tctcgtgcag cgccccgccc
     3541 aatacgcccg cacagacaaa ttcgcgctgg tttaaaactg tcagtatggc ttcacggtct
     3601 ttcttcttgt ccggggtatc tgatgtatag gtggcgagta tgcatccgtc tgtttttgcc
     3661 agcccgttta ctatggttat ggcttcttga agggcggcat tgttacgttc ggccatgatc
     3721 atgtcgatgg tggcgcgctt tttggcgttt tccgtattcc tcatgatgcc gtaagcggca
     3781 acaaaaacgc cgataacagt caatatcggc gttgctatct gtattaggtt gtcagtcatt
     3841 gccgtcccag ccgtccgaaa ggcggaaatc acggctttcc gaataaatat gccccattgt
     3901 catcccctta agctgatgtt gagaataaat ccttgcgtcg gaatatacgg cattgcctga
     3961 ctattttcaa gattccgcac cccgcggacc ggactataca aaaaaaccgc cttcgtgtaa
     4021 agcggttgaa gagagccttt caataaaaat gccgtccgaa gggctttcgg acggcatttt
     4081 ttatagattc ttttttaatg aacctttgaa accgaagata attaattttc ctctttattt
     4141 tcaatacgca gtgtgcccta tgtttttaaa taaaaatatt ttaatccaac atttttgcaa
     4201 tatccgccat gtcggggcgg tagtaaacat tgagtaaaat ccttaaatcc cgatgcccgc
     4261 tgatttttgc caagacctcc acaggcactt tttttgatag gcgcgtgagg gcttcgcggc
     4321 gggtgtcgtg aaaatgcagg ccttgtatgc cgcaattatc ccgcgctcgc ctgaataata
     4381 catcaaggct tttggcatcc aggctaaaca cccatgtgtc atcaattccg cgcagttgct
     4441 ctattagttc ggcggctcgc aaggataatg gcacgtcacg gctgtcgccg tttttagtta
     4501 ttgggaggtg ggcaatacga cgccgcatat tcacatctgc ccattttagg ccgcagattt
     4561 cgcctgcccg catggcggtt tcgatggcaa ataggacggc aacggcaacc cgttgcaccg
     4621 ccattttcgg tttctcatta ggccggtata ggagggcggc gcaaatatcg gcaatttcct
     4681 gctcggttgg ccgtcttgtc cttgcccggc tttttttcgg cttgctgatt ttgcgtacag
     4741 ggttttcgcg aagaagtccc cactctttca tggcgtgttc gcagacggcg gataaagtgg
     4801 ttaattcgcg tccgacgctg gtgggggata cctcctgcaa ccgttgatct cgccaatcgg
     4861 cgaagtcttg agggcgcagg tcggcaagcc gcacctttgc caaaggtgtt tttagtgcgc
     4921 gtccaatgcg gtatgattct tcccgtgcgc cccgctttga tggtgtaacc tcggataaat
     4981 accgccggat aaggtcggca aagatagtat ttcgcggggc attgccttgg atgccgtcca
     5041 taatttgcga ctctattcgc gccgcccacg ccttcgcgtc tgatgcccgc tcaaatgtgg
     5101 cggaacggga aacgcctttc atacggattt gtacgcgcca tttgccgtta cgttgagtga
     5161 tggttgccat atttattcgg gtgcaattcg ggtgcaaata taatagagca aaacagatat
     5221 aaagcggggc aaaatagggc tatattaaat ttaatgcttt gatatgtagg gataaataga
     5281 tttaaaaaga cgaaagaagt gcggacgtag aataagcgta gatgcgctcc gtccgcacca
     5341 ataaaaatat ataaatcata ttgttaatga ttattgggac aattcggtgg cacaaaatat
     5401 aagctgtctg cttttcagcg ggcggctttt tatctgccta tgtgtttcaa cacacaaaac
     5461 ggcacataaa gcaccgccat ttgttccgca ccgaatcgga agcgggtgct tccgcccgca
     5521 atcggattcg ccgcccctaa tgtcggggtt ttaaccggaa agaagaaaat attgattttg
     5581 atgatggcat caaaaaaacc cttccgcccg tctgccaaaa ggaatggtta agaaaagcaa
     5641 tgcgtctttt gatggaacgc gatatataag aaattgaggg gaagggcagg cgggtttctg
     5701 cccgttaatc ctgcggacgg ccccgccaaa cccgacacgc gcgggcatat ggcggattgt
     5761 ttacaatatt gtctaaaaat ctgaacctgc aaacgcgaaa taaggtagaa tacgccccgt
     5821 tattttaccg tccaatcccc atttaccaag gaaaaacgat gagtacttca ttgagttacc
     5881 gcgatgcggg cgtcggtatc gacgcaggcg accaactggt cgaaaaaatc aaaccgtttg
     5941 ccaaacgcac catgcgtccg gaagtattgg gggatttggg cggttttggc gcattggtcg
     6001 aaatcggcaa aaaatatcaa aaccccgtat tggtcagcgg tacggacggc gtgggtacca
     6061 agctcaaact tgccttcgat tgggacaaac acgatacggt gggcatcgac cttgttgcaa
     6121 tgagtgtcaa cgatattttg gttcaaggag ctgaaccatt gtttttcttg gattattttg
     6181 cctgcggtaa attggatgtt ccgcgtgcga ccgatgtcat caaaggcatt gcccaaggtt
     6241 gcgaagaatc cggctgcgcc ctgattggcg gggaaactgc cgaaatgccg ggtatgtatc
     6301 ccgttggtga atacgatttg gcgggttttg ccgtcggcgt ggtggaaaaa gagaatgtca
     6361 ttaccggccg cagcatcggc gcgggcgatg tggtattggg tttggcttcc aacggcgcac
     6421 attcaaacgg ctattccctt atccgtaaaa tcatcgaacg cgacaatccc gatctggatg
     6481 ccgagtttga taatggcaaa actttacgtg aagccgttat tgcgccgaca cgtctgtatg
     6541 tgaaaccgat tcttgccgct ttggaaaaat ttaccattaa aggtatggcg cacattaccg
     6601 gcggcggcat taccgaaaac gtgccgcgcg tgttgcctaa aaacacggtt gcccaaatcg
     6661 atgccgaatc gtgggaattg cccaagctct tccaatggct tcaaaaggcg ggcaatgtgg
     6721 aaacccaaga aatgtaccgg acctttaact gcggcatcgg tatggtcgtc atcgttgctg
     6781 ccgaagatgc cgatgcggtt cggtcattcc tgagcgggca gggcgagaca gtgtaccgtt
     6841 tgggttgcat ccgagagcgt caagggaacg agcatcaaac ccaagttgcc tgacttccaa
     6901 gcatcaaatg ccgtctgaat ttgtttcaga cggcatttcc gcatcttcca aacgcgtatc
     6961 cgcaacacag ttctgcaaac tgatattttg cccggtatgg cggcttaaac ttcttcatca
     7021 tatttcgata atgctataaa atgatataat cataaaatat tgtttattcc tacatcgtat
     7081 tcatcctatt ttccgaaatg tgagcgtttt atgaagcaat ccgtccataa taaaacaatt
     7141 ttaagtgttt tg
//
LOCUS       DAWTQS010000082         6918 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.082, whole genome shotgun sequence.
ACCESSION   DAWTQS010000082 DAWTQS010000000
VERSION     DAWTQS010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6918
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.082"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(1014..1697)
                     /locus_tag="WNA25_001885"
     CDS             complement(1014..1697)
                     /locus_tag="WNA25_001885"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFA9824578.1"
                     /translation="MELVFIRHGQSEWNAKNLFTGWRDVKLSEQGLAEAAAAGKKLKE
                     NGYEFDIAFTSVLTRAIKTCNIVLEESDQLFVPQIKTWRLNERHYGRLQGLDKKQTAE
                     KYGDEQVRIWRRSYDTLPPLLDKDDAFSAHKDRRYAHLPADVVPDGENLKVTLERVLP
                     FWEDQIAPAILSGKRVLVAAHGNSLRALAKHIEGISDEDIMGLEIPTGQPLVYKLDDN
                     LKVIEKFYL"
     gene            1846..4149
                     /gene="parC"
                     /locus_tag="WNA25_001886"
     CDS             1846..4149
                     /gene="parC"
                     /locus_tag="WNA25_001886"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFA9824579.1"
                     /translation="MNTQPHASHTDSNTLMLGRYAERAYLEYAMSVVKGRALPEVSDG
                     QKPVQRRILFAMRDMGLTAGAKPVKSARVVGEILGKYHPHGNSSAYEAMVRMAQDFTL
                     RYPLIDGIGNFGSRDGDGAAAMRYTEARLTPIAELLLSEINQGTVDFMPNYDGAFDEP
                     LHLPARLPMVLLNGASGIAVGMATEIPSHNLNEVTQAAIALLKKPTLETADLMQYIPA
                     PDFAGGGQIITPADELRRIYETGKGSVRVRARYEIEKLARGQWRVIVTELPPNANSAK
                     ILAEIEEQTNPKPKAGKKQLNQDRLNTKKLMLDLIDRVRDESDGEHPVRLVFEPKSSR
                     IDTDTFINTLMAQTSLEGNVSMNLVMMGLDNRPAQKNLKTILQEWLDFRIVTVTRRLK
                     FRLNQVEKRLHILEGRLKVFLHIDEVIKVIRESDDPKADLMAAFGLTEIQAEDILEIR
                     LRQLARLEGFKLEKELNELREEQGRLNIFLGDENEKRKLIIKEMQADMKQFGDARRTL
                     VEEAGRAVLTQTAADEPITLILSEKGWIRSRAGHNLDLSQTAFKEGDRLKQTLEGRTV
                     LPVVILDSSGRTYSIDAAEIPGGRGDGVPISSLIELQNGAKPVAMLTGLPEQHYLLSS
                     SGGYGFIAKLGDMVGRVKAGKVVMTADSGETVLPPVAVYASSFINPDCKIIAATSQNR
                     ALAFPIGELKIMAKGKGLQIIGLNAGESMTHTAVSSEPEILIESEGRRGAAHKDRLPV
                     ALIEAKRGKKGRLLPISGSLKQLSSPK"
     gene            4169..5893
                     /locus_tag="WNA25_001887"
     CDS             4169..5893
                     /locus_tag="WNA25_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HFA9824580.1"
                     /translation="MVISNPRELEKLKDRIPNLINIIRVAIVFPLMIMHILGLETGSR
                     ANLHASWTAWAFYLWLAIACWLIFFSTLNPQWQWQWQALRIPSFSAVADITMIGVLTY
                     LFGGIDSGFGILILPFVGSSCLLSYGRYPLLYASYASILLIFNALADSNINMYPLILD
                     AKTVTNTFVVVAGSYFVAMIASLSVRYIDRAGKLAHENHVAYRRIRGLNQIVLNRVQE
                     AVVVINVEHQTILFNKKAKDLLPMLEIGQHTSLFDPVAILWDKTSSRTFEHYIDTPEL
                     TARIRAVPMNKKQNKLLILYIRPQSEIQAEALSVKLAALGQLTANLAHEIRNPMSAIR
                     HADGGTLFLDEVADLPLSMQVKLLRAIQEKAVRRIGDATEQPVDVRIVCATHKNLEAL
                     VESGAFRQDLYYRLNVVSLNMPSLREMRENLKLPTPYLLYKHSHNNRPYTLSPAAQQM
                     LLNYSYPGNFRELENILERAVALCVGYTVQIDDLQIQDVHHKPVRTETAVPVADTLPS
                     ETAAAPSRLLPFDPDTMQIQDYLDKIERDIIGQVLKQTEGNRTQAAKRLGISFRSMRY
                     RMERLNID"
     gene            5993..6745
                     /locus_tag="WNA25_001888"
     CDS             5993..6745
                     /locus_tag="WNA25_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFA9824581.1"
                     /translation="MTDILIDDTATEAVRTLIRAFPLVPVSQPPEQGSYLLAEHDTVS
                     LRLVGEKSNVIVDFTSGAAQYRRTKGGGELIAKAVNHTAHPTVWDATAGLGRDSFVLA
                     SLGLTVTAFEQHPAVACLLSDGIRRALLNPETQDTAARINLHFGNAAEQMPALVKTQG
                     KPDIVYLDPMYPERRKSAAVKKEMAYFHRLVGEAQDEVILLHTARQTAKKRVVVKRPR
                     LGEHLAGQAPAYQYTGKSTRFDVYLPYGADKG"
BASE COUNT         1664 a         2057 c         1697 g         1500 t
ORIGIN      
        1 cagctaaaaa tcctgcggga tcggtgtgga atttagggat aatctagtac agcccctact
       61 attttagtag ttttaccatt agaaaaagga tatgaatgga attgccttga ggtagaagta
      121 ctatccgctg ctcaatctct agcaaatatc tattgcaaca aggttcgcct catagatggt
      181 catgtttggg aggaataaat gaaaaagcgt agttggtaat ttttagttgc gcagggtggg
      241 cttcagccca ccgttttttc ttcattttgg gttgttgatt ggtgggctaa agcccaccct
      301 acgattacta aaatcccgtc attcccgcgt agagcgcatc ataataaggc gggaatccag
      361 acctttcggt gcgaaaactt atcggataaa acggtttccc ctgcatcctg gattcccact
      421 ttcgtgggaa tgacgggata taggtttccc tacggacgtg ttcagattcc tgccttcgcg
      481 ggaataacgg cggagcagtt tctgcttttc caataaatga ccgtaactta aaatcccgtc
      541 atccccgcga aagcaaaaat cctgcctgtc ggatttcggt tttttcggga aactgatgaa
      601 tcgtcattcc cgcgcaggcg ggaatccaga acgcgggacg gcggcaatat tcaaaggttg
      661 tctgaaaatt cagaggttcc agattcccgc cttcgcggga atgacagcgg agcagtttct
      721 gcttttccga taaatgaccg caacctaaaa cccatccttc acacgaaaac agaaaaacaa
      781 aaacctaaaa tcccgtcatt cccgcgataa cagttgcgta attgcgtagg gtgggcttca
      841 gcccaccgtt tcttcatttt tgcttgtcaa ttagtggact gaagcccacc ctgcggttac
      901 tgtttcttct ttttcgatta ttaattggca ggctgaagcc ctccctacgt ttaccgtcaa
      961 aaatgccgtc tgaaatgctc aagccctcag acggcatttt ttatttcgca cccttacagg
     1021 taaaatttct cgatgacttt caggttgtcg tctaatttgt acaccagcgg ctggccggtc
     1081 gggatttcca agcccatgat gtcttcgtcg gaaatgccct cgatgtgttt tgccagcgcg
     1141 cgcagggagt tgccgtgcgc cgctaccaag acgcgtttgc cgctcaaaat cgcgggggcg
     1201 atttggtctt cccaaaacgg taatacgcgc tccagcgtta ctttcaggtt ttcgccgtcg
     1261 ggtacgacat cggcaggcag atgggcatag cgacggtctt tgtgtgcgga aaacgcgtcg
     1321 tctttgtcca aaagcggcgg cagggtgtcg tagctgcgcc gccagatgcg gacttgctcg
     1381 tcgccgtatt tttcggcggt ttgttttttg tccaaacctt gcagtcggcc gtagtggcgt
     1441 tcgttcagcc tccatgtttt gatttgcggt acgaacagtt ggtcggattc ttccaaaacg
     1501 atgttgcagg tcttaatcgc gcgggtcagg acggatgtga aggcgatgtc gaactcatag
     1561 ccgttttctt tcagtttttt accggcggcg gcggcctcgg caagcccctg ctcgctcagc
     1621 ttcacgtcgc gccagcctgt aaacaggttt ttcgcgttcc attcgctttg tccgtggcgg
     1681 ataaatacca attccatatc gtctccaatg tgtgaaagtg ggaaagcttt atttataaca
     1741 tattttcaca tttcccgtat ttgattcaga ttcaggcacg gcgcccacta tggtttgcca
     1801 ttttgattta caataatgtc ctttgcttta cattccgcat acacaatgaa tacgcaaccg
     1861 cacgcttccc ataccgattc caacacgctg atgctcggcc gatacgccga acgcgcctat
     1921 ctcgaatacg ccatgagcgt ggtcaaaggc cgcgcgctgc ctgaagtttc agacggccaa
     1981 aagcccgtgc agcggcgcat tttgtttgcc atgcgcgata tgggtttgac ggcgggggcg
     2041 aagccggtga aatcggcgcg cgtggtcggc gagattttgg gtaaatacca tccgcacggc
     2101 aacagttccg cctatgaggc gatggtgcgc atggctcagg attttacctt gcgctatccc
     2161 ttaatcgacg gcatcggcaa cttcggttcg cgcgacggcg acggggcggc ggcgatgcgt
     2221 tacaccgaag cgcggctgac gccgattgcg gaattgctat tgtccgaaat caatcagggg
     2281 acggtggatt ttatgccgaa ctacgacggc gcgtttgacg agccgctgca ccttcccgcc
     2341 cgcttgccta tggtgttgct caacggcgcg tcgggcatcg cggtgggtat ggcgaccgag
     2401 attccgtcgc acaatttgaa cgaagtcacg caggcggcga ttgcactgtt gaagaaaccg
     2461 acgctggaaa ccgccgacct gatgcaatat attcctgctc ccgattttgc cggcggcggt
     2521 caaatcatca cgccggcgga cgaattgcgc cgtatttacg aaaccggcaa gggcagcgtg
     2581 cgcgtgcgtg cgcgttatga aatcgagaaa ttggcgcgcg gacagtggcg cgtcatcgta
     2641 accgaactgc cgccgaacgc caactccgcc aaaatccttg ccgaaatcga agagcaaacc
     2701 aacccgaaac cgaaagcggg caagaagcag ctcaaccaag accggcttaa taccaaaaag
     2761 ctgatgctgg atttaatcga ccgcgtgcgc gacgagtccg acggcgaaca tcccgtgcgc
     2821 cttgtatttg aaccgaaatc cagccgcatc gataccgata ccttcatcaa cacgctgatg
     2881 gcgcaaactt cgctggaagg caatgtgtcc atgaacttgg tgatgatggg tttggacaac
     2941 cgccccgcgc agaaaaacct gaaaacgatt ttgcaggaat ggctggattt ccgcatcgtt
     3001 accgtaacac gccgtctgaa attccgttta aaccaagtgg aaaaacggct gcacatcctc
     3061 gaaggccgtc tgaaagtctt tctgcacatc gacgaagtga ttaaagtcat ccgcgaatcg
     3121 gacgacccga aagccgattt gatggcggca ttcgggctga ccgaaatcca ggccgaagac
     3181 attttggaaa tccgcctgcg tcagctggcg cgtttggaag gtttcaaact cgaaaaagaa
     3241 ttgaacgaat tgcgcgaaga acaaggccgt ctgaatatct ttttgggcga cgaaaacgaa
     3301 aaacgcaagc tgattatcaa agagatgcag gcggacatga agcagttcgg cgacgcgcgc
     3361 cgcacgctgg tggaagaggc cggacgcgcc gtgctgacac aaaccgccgc cgacgaaccc
     3421 atcacgctga ttttgtcgga aaaaggctgg atacgcagcc gtgccggaca taatctcgat
     3481 ttgagccaaa ccgcgttcaa agaaggcgac cgcctcaaac aaacccttga aggccgcact
     3541 gttttacccg tcgtcatcct cgattcatcg ggcagaacct actcgatcga tgccgccgaa
     3601 atccccggcg gacgcggcga cggcgtaccg atttcctcct taatcgagtt gcaaaacggc
     3661 gcgaaacccg tcgcgatgtt gacaggattg ccggaacaac attatttatt atcaagcagc
     3721 ggcggctacg gctttatcgc caagctgggc gatatggtcg gacgcgtgaa agcgggcaaa
     3781 gtggtgatga ccgcagacag cggcgaaacc gtcctgccgc cggttgccgt ctatgcctcc
     3841 tcgttcatca accccgactg caaaatcatt gcagccacca gtcaaaaccg cgccctcgcc
     3901 ttccccatcg gcgaattgaa aattatggcg aaaggcaaag gactgcaaat catcggatta
     3961 aacgccggcg aatcgatgac gcataccgcc gtttcttccg agccggaaat cctgattgaa
     4021 agcgaaggca ggcgcggcgc ggcgcacaaa gaccgcctcc ccgtcgccct gattgaggca
     4081 aaacgcggca aaaaaggcag actgttgccc atatcgggca gcctgaaaca gctttcttcc
     4141 cccaaataaa cccggttccg cacatattat ggtgatttcc aacccccgcg aacttgaaaa
     4201 actcaaagac cggattccca atctgatcaa catcatccgc gtcgccatcg ttttcccgct
     4261 gatgattatg cacatcctcg ggctggaaac cggcagccgt gcgaacctgc acgcctcatg
     4321 gacggcgtgg gcgttttact tgtggcttgc cattgcctgc tggctgattt tcttttccac
     4381 cctcaacccg caatggcaat ggcaatggca ggctttgaga ataccgagtt tcagcgccgt
     4441 ggccgacatc acgatgatcg gcgtgctgac ctacctgttc ggcggcatcg attcggggtt
     4501 cggcatcctg atcctgccct tcgtgggcag ctcctgccta ctcagttacg ggcgttatcc
     4561 cctgctctat gccagctatg catccatcct gctgatattc aacgcccttg cagacagcaa
     4621 tatcaatatg tatccgctga ttctggatgc aaaaaccgtc accaatacct tcgtcgtcgt
     4681 agccggctcc tatttcgtcg ctatgattgc ctcgctgtcg gtcagataca tcgaccgtgc
     4741 cggcaaactt gcccatgaaa accacgtcgc ctaccgccgt atcaggggct tgaaccaaat
     4801 cgtgctcaac cgcgttcagg aagcggtcgt cgtcatcaac gtcgagcatc agaccatact
     4861 gttcaataaa aaggcaaaag atctgctccc catgcttgaa atcggacagc atacctccct
     4921 gttcgaccct gtcgccatct tatgggataa aacatcctca cgcaccttcg aacactatat
     4981 cgacacgccc gaactgaccg cccgcatccg cgccgtgccg atgaacaaaa agcagaacaa
     5041 gctgctcatc ctctatatcc gcccgcaaag cgaaattcag gcggaagccc tgtccgtcaa
     5101 acttgccgcg ctcggacaac tgaccgccaa ccttgcccac gaaatccgca acccgatgtc
     5161 cgccatccgc cacgccgacg gcggcacttt atttttggac gaagtggccg acctgcccct
     5221 ttccatgcag gtcaaactct tgcgcgcgat tcaagaaaaa gccgtgcgcc gtatcggcga
     5281 cgcgaccgag cagcccgtcg atgtccgcat cgtctgcgcc acccacaaaa acctcgaagc
     5341 ccttgtcgaa agcggcgcat tccgccaaga cctgtattac cgtctcaatg tcgtcagcct
     5401 caatatgccg tccctgcgtg aaatgcgcga aaatttgaag ctgcccaccc cctacctcct
     5461 gtacaaacac agccacaaca accggcccta cacactctcc cccgccgcgc aacagatgct
     5521 cctgaattac agttatccgg gcaatttccg cgaactcgaa aacatcctcg aacgcgccgt
     5581 cgccctgtgc gtcggatata cagtgcaaat cgacgacctg caaatccaag atgtgcacca
     5641 caaacccgtc cggacggaaa ccgccgtccc cgttgccgat accttgccgt ctgaaacagc
     5701 cgccgcaccg tcccgccttc tcccgttcga ccccgatacc atgcagatac aggactatct
     5761 cgacaaaatc gaacgcgaca tcatcggaca agtcctcaaa caaaccgaag gcaaccgcac
     5821 gcaagccgcc aaacgcttgg gcatcagctt ccgctctatg cgctaccgta tggaacgcct
     5881 caacatagac tgacaaacgg catccgcacc atctccgccc acccgaaaaa atgccgtctg
     5941 aaacggcacg gggaagcggg ttcgccccac gaccgaacgg acacaataca ccatgaccga
     6001 catcctcatt gacgataccg ccaccgaagc cgtccgcacc ctgatacggg cattccccct
     6061 cgtgcccgtt tcccaaccgc ccgaacaagg cagttacctc cttgctgaac acgataccgt
     6121 cagcctcagg cttgtcgggg aaaaaagcaa cgtcatcgtc gattttacct ccggcgcggc
     6181 acaataccgg cgcacaaaag gcggaggaga actcatcgcc aaagccgtca accacaccgc
     6241 gcaccccacc gtatgggatg caaccgcagg attggggcgc gacagcttcg tcctcgcctc
     6301 gctcggactg accgttaccg ccttcgagca acatcccgcc gtcgcctgcc tgctttcaga
     6361 cggcatccgc cgcgccctcc tcaatcccga aacgcaagac accgccgcgc gcatcaacct
     6421 ccatttcggc aatgccgccg aacaaatgcc tgcccttgtt aaaacacaag gcaaacccga
     6481 catcgtttat ctcgacccga tgtatcccga acgccgcaaa agtgccgccg tcaaaaaaga
     6541 aatggcttat ttccaccggc ttgtcggcga agcgcaagat gaggtcatcc tccttcatac
     6601 cgcacgccaa acggcaaaaa aacgcgtcgt cgtcaaacgc ccgcgcctcg gcgaacacct
     6661 tgccggacaa gcccccgcct accaatacac cggcaaaagc acccgcttcg acgtttacct
     6721 gccctacgga gcggacaagg gataacgccc ataaaacaag acaccgaaaa atttgccgtt
     6781 cttatgcaaa cgagaaaccg gtttttgcgt ttcgactgtt ttggataagt catcacacct
     6841 taaagtttgt cattcccacg gaagtgggaa tccgattcgt tcagttttag ttgttttcaa
     6901 gtttcaggca acttccaa
//
LOCUS       DAWTQS010000083         6573 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.083, whole genome shotgun sequence.
ACCESSION   DAWTQS010000083 DAWTQS010000000
VERSION     DAWTQS010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6573
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.083"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(61..258)
                     /locus_tag="WNA25_001889"
     CDS             complement(61..258)
                     /locus_tag="WNA25_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824582.1"
                     /translation="MQKVYVVQSVSTGDFLYLSPETGDIGHTKLITNADYFYDFEEAV
                     NAGLEEIGNQCEFVVFGFLKD"
     gene            complement(263..553)
                     /locus_tag="WNA25_001890"
     CDS             complement(263..553)
                     /locus_tag="WNA25_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824583.1"
                     /translation="MNIQLQGHIVGVKKFNGQIEGKSFDYCRLIVATPLDSSQGNALG
                     SSTTEYDFGGSANFEQFRNAQFPIEANLNVEIVTTGKTQKLKVTGFQFVKKG"
     gene            complement(598..1946)
                     /locus_tag="WNA25_001891"
                     /pseudo
     CDS             complement(598..1946)
                     /locus_tag="WNA25_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            complement(2085..2507)
                     /locus_tag="WNA25_001892"
     CDS             complement(2085..2507)
                     /locus_tag="WNA25_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HFA9824584.1"
                     /translation="MTDIFTPSKRSFVMSKIHSKETKPEVLVRKFLFSQGFRYRKNDK
                     RYAGKPDIVLPKYKTVVFIHGCFWHGHSCNKGHIPKSNMDFWLEKITKNRERDIKNET
                     ELEKIGFKVIVVWECELKNKAICRERLNRLVEEIKDAV"
     gene            complement(2513..4156)
                     /locus_tag="WNA25_001893"
                     /pseudo
     CDS             complement(2513..4156)
                     /locus_tag="WNA25_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237377.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02391 family protein"
     gene            complement(4170..4499)
                     /locus_tag="WNA25_001894"
     CDS             complement(4170..4499)
                     /locus_tag="WNA25_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFA9824585.1"
                     /translation="MAFAHIPELQLECFIGKDNSFKDTFGRLWWDKPAPTITTKFFSI
                     SNGRFAHPEEDRALSLREGATLQSFPRNYVFKAGSRDKIARLIGNAVPPMYAEKIGRA
                     IVDNIEC"
     gene            complement(4538..5146)
                     /locus_tag="WNA25_001895"
     CDS             complement(4538..5146)
                     /locus_tag="WNA25_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFA9824586.1"
                     /translation="MQVLAGIDYELSCKETYEANINAKFIQADIFELQPETLEKELGL
                     KKNDDDLILIGCSPCQYWSVIQTDKRKSEKSKSLLLEFQRFVEYFNPGYVVVENVPGI
                     LSRMKESGLDNFIKLLEEKGFTVHFGIHNTADYGIPQSRKRFTLIANRITKKSWNQSS
                     IRANGLRYAMFWEWKTAFPKLWQDIKTRRILCIVVRDYPISI"
     gene            5515..5661
                     /locus_tag="WNA25_001896"
     CDS             5515..5661
                     /locus_tag="WNA25_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824587.1"
                     /translation="MSRIYLPLLFPPHIVERGLLYFQQDKVRDVQKISLGRYRAKVFD
                     SENY"
     gene            complement(6038..6487)
                     /locus_tag="WNA25_001897"
     CDS             complement(6038..6487)
                     /locus_tag="WNA25_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HFA9824588.1"
                     /translation="MTDNRGFTLVELISVVLILSVLALIVYPSYRNYVEKAKINAVRA
                     ALLENAHFMEKFYLQNGRFKQTSTKWPSLPIKEAEGFCIRLNGIARGALDSKFMLKAV
                     AIDKDKNPFIIKMNENLVTFICKKSASSCSDGLDYFKGNDKDCKLLK"
BASE COUNT         1779 a         1504 c         1178 g         2112 t
ORIGIN      
        1 aaaaagtgtt tggcggtaat ggatggagag tttttcagac gaccgccgaa catccgaaaa
       61 tcagtctttc aaaaatccga atacgacaaa ttcgcattgg ttgccgattt cttccaaacc
      121 tgcgttaacc gcttcttcga agtcgtagaa ataatcggca ttggtaatta atttggtatg
      181 tccgatgtcg cccgtttcag gagagagata cagaaagtcc cctgttgata cggactggac
      241 aacatagact ttctgcattc aatcagcctt tcttaacgaa ttgaaaaccg gtgactttca
      301 gtttttgagt tttgcccgta gtgacgattt ccacgttcag gtttgcttcg atcggaaatt
      361 gggcgtttcg gaactgctcg aaattggcag agccgccgaa atcgtattca gtagtagagc
      421 tgcccaatgc gttgccttgg gagctgtcta agggtgtggc gacaatcagg cggcaatagt
      481 cgaagctctt gccttcgatt tgtccgttga attttttaac gccgacgatg tggccttgaa
      541 gttggatgtt cattttttgg tttccttgtg tgattaaacg tctttcgggc agacacttta
      601 agcccatgaa atcggtagtc ttgcgaattt gtcgtaaatg aagttgttat agctttcttc
      661 attgttgacg tgtttttgct gttcaagctg tttttcaaga ttctcgtaat attcgtacat
      721 ataataaggg tcattgtacg gtttgaatgc gggctgttca tgaatgactt gagatttcaa
      781 aaaggcgcag tcgtaggctt cgagagccaa agacttgggc agcttgtgat gactcggctc
      841 aatcagttca aacagtttgg ctttgtccaa ttcgggaaaa atgaatttca gaccgtttgc
      901 cgcacgtccg aactgttttt ttacccattc aaggcagcgg tcggctgaaa caaccttatc
      961 ttccttaacc gcgtgtatgc gcgttgcctt ttgggcgaat cgttcgcaaa tgggatatgc
     1021 gccgccgaaa tattcgcccg gattctgcaa aacttcgaaa gggataacga tgtcttttgc
     1081 tttgaattca atttcaaagc gcgtccatgt gcttgtttta tcgcccaact gcttgccttt
     1141 ttcatagacg cggacatatt tggacgattc acgggagccg ataccatagg tcttgccttt
     1201 ggtcattttg gcttcatcgt cttcttccca atccgacccc aaacattcgc ccttcggttt
     1261 gacgtgatgg cacgtaaaca aacctttatt gcggtcttct cttgcctgat tcgggctgta
     1321 ctctccgttg aaaaagtctt ttgcaatgtc aacgcgcgtt atttttggac gaatcgcatt
     1381 tgtcagaaaa tcaaaaagcc ttgattccca tccaatattt gcagcattgc agcctaccgc
     1441 cgtcaattca accaacatcg tttcacgttg gccgccgtaa tggacgcgcc cgtattgggc
     1501 attttccgtt cccatcagcc agcagccttc atagaaacgc ccgcccgaat gcttggcttt
     1561 ttcaatgatt ccgaatccga aaatttcctc catcttggcg gaagccgcgc gtatgaaatc
     1621 gtcatcatcg aacaaatcaa acgaaagtcc ataaacatgg aaaaacgtgt cttcatgaat
     1681 tgaaaatgtg atttggtcaa tgaaagccga atctgataca ccgcgcctaa gaggaacgcc
     1741 taacaggttt cctttaccgt ccgttatgta cgtttcgtaa cattcgaaga cttcctgaac
     1801 cctgcccgcc gtttcggttt ctgtgccccc ccctgttaga taaggggggg gggaagattc
     1861 gaagcggttg ccggcttcct gccgtccgcc ggcgcctccg tcatcacgcc ggcaacctcc
     1921 tttgtcatcc cttgcttatc ttccatttgc gaatcctcaa aaacgggcaa aaaaaaagcc
     1981 ctgttacttg tagaaagtaa agggcgttaa tttttgttaa tcaccccttc ttagggacgc
     2041 aatatataag ggtcttaatt aaaggaagat gccgtctgat tttttcagac ggcatctttt
     2101 atttcctcga ccaaccgatt taatcgctcc ctacaaatag ctttattttt taattcgcac
     2161 tcccaaacga caatcacttt gaaaccaatc ttttccagct cggtttcatt tttaatatca
     2221 cgttcgcgat tttttgtgat tttttccaac caaaaatcca tattgctttt cgggatatgt
     2281 cctttattac aagaatgccc gtgccaaaaa cagccgtgta taaacacgac tgttttatat
     2341 ttaggcaaaa cgatatccgg tttgcctgca tagcgcttat cgtttttccg ataacggaaa
     2401 ccttgggaaa aaagaaattt ccttaccaat acttccggtt tggtttcttt gctatggatt
     2461 tttgacatca cgaaggaacg tttggatgga gtgaaaatat cggtcataaa atttaaggcg
     2521 atgtaggttt agctcgtttt tttgtttgtt ccacttttct caataaatat gaaactaagc
     2581 tgattaaatc caaagcatcc ttgtcattaa aaatactagg agataaattt gctcttaact
     2641 catgttgcac aggatttcta aatccagtcc atgttccgca tgcaagctgt tcaagaccat
     2701 cttctaaatt ttgttccgcc tgcgttttgt tattcgttaa tagaagcata gattttttac
     2761 tcccgaaggc tttttcaatc aattctcttc cgtctatgct atgaagacct gtctgcttct
     2821 gaaccttatc ttcgaaaact ttaactgcct cgattgcagc acttaaataa tccttctttt
     2881 cattgtaaaa atcgttacag gcagtatgca aatcctgatg caaatgccgc caatgatagt
     2941 aaggatattc tggaataata ctatgtaagg cagagattac ttccgcttgt tctttatttg
     3001 taagctccga atcttccaaa cgattcaaaa taacttctac cggttctctt tccttttctg
     3061 gcaatttgtt tttccaattt tcgatatcaa ctccgctttc acctttcact tcgtaatctt
     3121 tctctttttt tcttcttttt ctccaatctt gcccgatggc tcagacgatt tccccaagga
     3181 aaacccgtaa ttttgaagtt ttaggatgct tccaatccaa tgatgtccta tctgtagcga
     3241 ttaaatcttc accatcatta tctataaaat caatacttaa ccatcctgtc agataggatt
     3301 agaaatggct ggattcgcta tcagtgaaaa actcgggcat atttaccatt ctgccgttgg
     3361 caaacaaggt aatacctttc atattattct ttaaaggttt ttccgttgta atgaacttac
     3421 cttgtattaa tccgctgtat tcatactgtt cgaattttga agataacgat gaaatattgg
     3481 ttgctagatc ctggtattcc cattcgaatt gtggggtaac aagattatat tttaggtttc
     3541 cgtcaatgat tcttggttcc gagccgttca agcttacttt gattttaaaa tcctgagccg
     3601 gaaaatcaaa taaacgggaa agatgcccca cataattatc taacgggtat ccttgttttt
     3661 tcgtcagctc ggttaaagtt atagttgttc cgttttcggt attgggttta acagactctt
     3721 tctgaaactc cggttgataa atacgctcac tttttttaat ctctgcataa tccaaagtaa
     3781 aagtaacccg ttcgtttcct tggatagtag agatttcgat tttgttgcca agcctgaata
     3841 atgccaattt accaagacct tttttacccg ttggaattct tccgcacggg gaggcttgtt
     3901 tttcttccct tctgttccga ccgattctca aataaaaatc attgatttca tcgaagctca
     3961 ttcctatgcc attatcttta ataacgattt tatgctccgg tttatcgaat aacctaactt
     4021 ccacttccgt agcacatgca tcgtaggcat tcgctatcaa ctcggcaatc gcaggaacag
     4081 tgtgcgaata catctttaca cccaaatgtt cgattacggt tggctcgaaa ctcattttca
     4141 atttttcttc agacatgttt tctccttttc taacattcga tattatcaac aattgccctg
     4201 cctatttttt ccgcatacat cggaggaacg gcattaccaa tcaaacgggc tatcttgtcc
     4261 ctactgcccg ctttaaagac atagttacga gggaacgatt gcaatgttgc accttctcgc
     4321 agggataacg ccctatcttc ttcaggatgg gcaaaccgcc cgttggaaat gctaaagaat
     4381 ttggtcgtta tggtaggtgc gggcttatcc caccatagcc taccgaacgt gtctttaaaa
     4441 ctattgtctt ttccgataaa acattccagc tgtaattcgg gaatatgcgc aaaagccaaa
     4501 cggtttcctc cgtttttagg tatcaaagcc aatcgtttca aattgatatc ggataatccc
     4561 gcacaactat gcataaaatc cgtctcgtct tgatgtcctg ccataatttt gggaaagccg
     4621 ttttccattc ccaaaacatc gcgtaccgta agccgtttgc ccgaatactt gactggttcc
     4681 agcttttttt ggttattctg tttgcaatta acgtaaatct tttacggctt tggggaattc
     4741 cgtaatcagc cgtattgtga ataccgaaat ggacggtaaa tcctttttct tccaataact
     4801 ttatgaaatt atcaagcccg ctttctttca ttctgctcag aattcccggt acgttctcta
     4861 caacaacata tccgggattg aaatattcta caaaccgctg aaactccaaa agcaaactct
     4921 tggatttttc cgattttctt ttgtctgttt gaataacact ccaatattga caaggactac
     4981 aacctataag aatcaaatca tcatcattct tttttaagcc gagttctttt tccaaggttt
     5041 caggctgcaa ttcaaaaata tcagcctgta taaatttggc atttatattg gcttcatatg
     5101 tttctttgca gctaagttcg taatctatcc cggctaaaac ctgaataccg gcggattaca
     5161 ttccatagct cattccgcct ccgccacaga aaaaatcaac cgcctttaac ttactgtttt
     5221 tcatttctat ttaaataatt agactagtga gaactaactt tatataacat ataatttatt
     5281 gcttatcaaa aataatttga gattttatta aaattcatgc atctaaataa gcagaactta
     5341 atttcctcca aacgatcgga tatcttcaaa tgcacatttc acagaaaggt cgtctgaaac
     5401 ccattttcag acgacctttt tccacttcgc aaaacaagaa ttcccacccc tctccccaaa
     5461 catcgcccaa accagtaaat tccccatatt tcaacaactc cttggaagca aaccatgtcc
     5521 cgtatctacc tacccctcct atttccgccc cacatcgtcg aacgcggcct tttgtatttt
     5581 cagcaggaca aggttcgcga tgtccaaaaa atttccttag ggcgttatcg ggcgaaggta
     5641 ttcgattcgg aaaactatta ggcatggttg aagctggata gtgatttgta cattaaggac
     5701 ggggctgcga ttgcccttat gtctaaaggt gcaaacatgc cttaaattac taagcctata
     5761 acgaattgga aacaaaatgc cgtctgaaaa catcttcaga cggcattata aaatctgttc
     5821 accttttcag atgagtaatg tacaccccta tacaattttt gctaccatgc accataaatc
     5881 cacggctaaa gataattggg tttattcttt tatccagaaa caatccagat acggatcacg
     5941 ggtcataact ataggcttaa tattacacga ttctcattcc atcaaggcgg aaaaccgcac
     6001 aaatactgaa acactatcga tcgatttgta aacaagccta cttaagtaac ttgcagtcct
     6061 tatcatttcc tttaaaataa tccagcccgt cactacacga actggcggac ttcttgcaaa
     6121 taaaggttac tagattttca ttcatcttaa taataaaagg atttttatct ttatctatgg
     6181 ctaccgcctt caacatgaat ttactgtcta aagccccgcg cgcgattcca ttcaaacgga
     6241 tacaaaagcc ttctgcctct ttaatcggca aacttggcca tttggtagat gtttgtttaa
     6301 atctcccatt ctgcagataa aacttttcca taaaatgtgc attttctaac aaggctgccc
     6361 gcactgcatt tatctttgct ttctcaacat aattgcgata gctcggataa acaattaaag
     6421 caagtacaga caatatcaag accactgata ttaattcaac cagcgtaaac ccccgattat
     6481 cagtcattac tttacttcca ataagaacag attattcaac atatttcttt gaacagactt
     6541 actatcccat tcagcagtat gcatatttcc cac
//
LOCUS       DAWTQS010000084         6494 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.084, whole genome shotgun sequence.
ACCESSION   DAWTQS010000084 DAWTQS010000000
VERSION     DAWTQS010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6494
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.084"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            240..1780
                     /locus_tag="WNA25_001898"
     rRNA            240..1780
                     /locus_tag="WNA25_001898"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1881..1957
                     /locus_tag="WNA25_001899"
     tRNA            1881..1957
                     /locus_tag="WNA25_001899"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1915..1917,aa:Ile,seq:gat)
     gene            1963..2038
                     /locus_tag="WNA25_001900"
     tRNA            1963..2038
                     /locus_tag="WNA25_001900"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1996..1998,aa:Ala,seq:tgc)
     gene            2385..5276
                     /locus_tag="WNA25_001901"
     rRNA            2385..5276
                     /locus_tag="WNA25_001901"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            5373..5486
                     /gene="rrf"
                     /locus_tag="WNA25_001902"
     rRNA            5373..5486
                     /gene="rrf"
                     /locus_tag="WNA25_001902"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            5776..6138
                     /locus_tag="WNA25_001903"
     CDS             5776..6138
                     /locus_tag="WNA25_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFA9824589.1"
                     /translation="MLIRHIVARCGLLFATLKGKTMKKMFVLFCMLFSCAFSLAAVNI
                     NAASQQELEALPGIGPAKAKAIAEYRAQNGAFKSVDDLIKVKGIGPAVLAKLKDQASV
                     GAPAPKGPAKPVLPAVKK"
BASE COUNT         1732 a         1419 c         1921 g         1422 t
ORIGIN      
        1 cgctgccgaa gcggcggacg aactgaaaag tatagcacgg aatgtcggaa atatcgagag
       61 atatcttaga caggcggaag ggatacttta taatttcgca acgctcttta acaaaacaga
      121 ttaccgataa gtgtgagtgc cttgggcctc gcactgtttg aaagacagac gagatgatgt
      181 tttgaacatt gtcctgtcgg tttctttgaa gcagaccaga agttaaaaag ttagagattg
      241 aacataagag tttgatcctg gctcagattg aacgctggcg gcatgcttta cacatgcaag
      301 tcggacggca gcacagggaa gcttgcttct cgggtggcga gtggcgaacg ggtgagtaac
      361 atatcggaac gtaccgggta gcgggggata actgatcgaa agatcagcta ataccgcata
      421 cgtcttgaga gggaaagcag gggaccttcg ggccttgcgc tatccgagcg gccgatatct
      481 gattagctgg ttggcggggt aaaggcccac caaggcgacg atcagtagcg ggtctgagag
      541 gatgatccgc cacactggga ctgagacacg gcccagactc ctacgggagg cagcagtggg
      601 gaattttgga caatgggcgc aagcctgatc cagccatgcc gcgtgtctga agaaggcctt
      661 cgggttgtaa aggacttttg tcagggaaga aaaggccgtt gccaatatcg gcggccgatg
      721 acggtacctg aagaataagc accggctaac tacgtgccag cagccgcggt aatacgtagg
      781 gtgcgagcgt taatcggaat tactgggcgt aaagcgggcg cagacggtta cttaagcagg
      841 atgtgaaatc cccgggctca acccgggaac tgcgttctga actgggtgac tcgagtgtgt
      901 cagagggagg tggaattcca cgtgtagcag tgaaatgcgt agagatgtgg aggaataccg
      961 atggcgaagg cagcctcctg ggataacact gacgttcatg tccgaaagcg tgggtagcaa
     1021 acaggattag ataccctggt agtccacgcc ctaaacgatg tcaattagct gttgggcaac
     1081 ttgattgctt ggtagcgtag ctaacgcgtg aaattgaccg cctggggagt acggtcgcaa
     1141 gattaaaact caaaggaatt gacggggacc cgcacaagcg gtggatgatg tggattaatt
     1201 cgatgcaacg cgaagaacct tacctggttt tgacatgtgc ggaatcctcc ggagacggag
     1261 gagtgccttc gggagccgta acacaggtgc tgcatggctg tcgtcagctc gtgtcgtgag
     1321 atgttgggtt aagtcccgca acgagcgcaa cccttgtcat tagttgccat cattcggttg
     1381 ggcactctaa tgagactgcc ggtgacaagc cggaggaagg tggggatgac gtcaagtcct
     1441 catggccctt atgaccaggg cttcacacgt catacaatgg tcggtacaga gggtagccaa
     1501 gccgcgaggc ggagccaatc tcacaaaacc gatcgtagtc cggattgcac tctgcaactc
     1561 gagtgcatga agtcggaatc gctagtaatc gcaggtcagc atactgcggt gaatacgttc
     1621 ccgggtcttg tacacaccgc ccgtcacacc atgggagtgg gggataccag aagtaggtag
     1681 ggtaaccgca aggagcccgc ttaccacggt atgcttcatg actggggtga agtcgtaaca
     1741 aggtagccgt aggggaacct gcggctggat cacctccttt ctagagaaag aaggggcttt
     1801 aggcattcac acttatcggt aaactgaaaa gatgcggaag aagcttgagt gaaggcaagg
     1861 ttcgcttaag aagggaaacc gggtttgtag ctcagctggt tagagcacac gcttgataag
     1921 cgtggggtcg gaggttcaag tcctcccaga cccaccaaga acgggggcat agctcagttg
     1981 gtagagcacc tgctttgcaa gcagggggtc atcggttcga tcccgtttgc ctccaccaaa
     2041 actttacaaa tgaaagcaag tttgctgttt ttagcagctt attttgattt gcgaagtaga
     2101 ataacgacgc atcgatcttt aacaaattgg aaagccgaaa tcaacaaaca aagacaatga
     2161 gtttgttttg attttttatt ctttgcaaag gataaaaacc tctcgcaaga gaaaagaaaa
     2221 caaacatagt atttgggtga tgattgtatc gacttaatcc tgaaacacaa aaggcaggat
     2281 taaggcacaa caaagcagta agctttatca aagtagggat ttcaagtttg cttacttagt
     2341 caacgggtag gtaaacgaag tcaaagaagt tcttgaaatg atagggtcaa gtgaataagt
     2401 gcatcaggcg gatgccttgg cgatgatagg cgacgaagga cgtgtaagcc tgcgaaaagc
     2461 gcgggggagc tggcaataaa gctatgattc cgcgatgtcc gaatggggaa acccactgca
     2521 ttctgtgcag tatcctaagt tgaatacata ggcttagaga agcgaacccg gagaactgaa
     2581 ccatctaagt acccggagga aaagaaatca accgagattc cgcaagtagt ggcgagcgaa
     2641 cgcggaggag cctgtacgta ataactgtcg agatagaaga acaagctggg aagcttgacc
     2701 atagcgggtg acagtcccgt attcgaaatc tcaacagcgg tactaagcgt acgaaaagta
     2761 gggcgggaca cgtgaaatcc tgtctgaata tggggggacc atcctccaag gctaaatact
     2821 catcatcgac cgatagtgaa ccagtaccgt gagggaaagg cgaaaagaac cccgggaggg
     2881 gagtgaaaca gaacctgaaa cctgatgcat acaaacagtg ggagcgccct agtggtgtga
     2941 ctgcgtacct tttgtataat gggtcaacga cttacattca gtagcgagct taaccggata
     3001 ggggaggcgt agggaaaccg agtcttaata gggcgatgag ttgctgggtg tagacccgaa
     3061 accgagtgat ctatccatgg ccaggttgaa ggtgccgtaa caggtactgg aggaccgaac
     3121 ccacgcatgt tgcaaaatgc ggggatgagc tgtgggtagg ggtgaaaggc taaacaaact
     3181 cggagatagc tggttctccc cgaaaactat ttaggtagtg cctcgagcaa gacactgatg
     3241 ggggtaaagc actgttatgg ctagggggtt attgcaactt accaacccat ggcaaactca
     3301 gaataccatc aagtggttcc tcgggagaca gacagcgggt gctaacgtcc gttgtcaaga
     3361 gggaaacaac ccagaccgcc ggctaaggtc ccaaatgata gattaagtgg taaacgaagt
     3421 gggaaggcac agacagccag gatgttggct tagaagcagc catcatttaa agaaagcgta
     3481 atagctcact ggtcgagtcg tcctgcgcgg aagatgtaac ggggctcaaa tctataaccg
     3541 aagctgcgga tgccggttta ccggcatggt aggggagcgt tctgtaggct gatgaaggtg
     3601 cattgtaaag tgtgctggag gtatcagaag tgcgaatgtt gacatgagta gcgataaagc
     3661 gggtgaaaag cccgctcgcc gaaagcccaa ggtttcctac gcaacgttca tcggcgtagg
     3721 gtaagtcggc ccctaaggcg aggcagaaat gcgtagtcga tgggaaacag gttaatattc
     3781 ctgtacttga ttcaaatgcg atgtggggac ggagaaggtt aggttggcaa gctgttggaa
     3841 tagcttgttt aagccggtag gtggaagact taggcaaatc cgggttttct taacaccgag
     3901 aagtgatgac gagtgtctac ggacacgaag caaccgatac cacgcttcca ggaaaagcca
     3961 ctaagcttca gtttgaatcg aaccgtaccg caaaccgaca caggtgggca ggatgagaat
     4021 tctaaggcgc ttgagagaac tcgggagaag gaactcggca aattgatacc gtaacttcgg
     4081 gagaaggtat gccctctaag gttaaggact tgctccgtaa gccccggagg gtcgcagaga
     4141 ataggtggct gcgactgttt attaaaaaca cagcactctg ccaacacgaa agtggacgta
     4201 tagggtgtga cgcctgcccg gtgccggaag gttaattgaa gatgtgcaag catcggatcg
     4261 aagccccggt aaacggcggc cgtaactata acggtcctaa ggtagcgaaa ttccttgtcg
     4321 ggtaagttcc gacccgcacg aatggcgtaa cgatggccac actgtctcct cccgagactc
     4381 agcgaagttg aagtggttgt gaagatgcaa tctacccgct gctagacgga aagaccccgt
     4441 gaacctttac tgtagctttg cattggactt tgaagtcact tgtgtaggat aggtgggagg
     4501 cttggaagca gagacgccag tctctgtgga gtcgtccttg aaataccacc ctggtgtctt
     4561 tgaggttcta acccagaccc gtcatccggg tcggggaccg tgcatggtag gcagtttgac
     4621 tggggcggtc tcctcccaaa gcgtaacgga ggagttcgaa ggttacctag gtccggtcgg
     4681 aaatcggact gatagtgcaa tggcaaaagg tagcttaact gcgagaccga caagtcgggc
     4741 aggtgcgaaa gcaggacata gtgatccggt ggttctgtat ggaagggcca tcgctcaacg
     4801 gataaaaggt actccgggga taacaggctg attccgccca agagttcata tcgacggcgg
     4861 agtttggcac ctcgatgtcg gctcatcaca tcctggggct gtagtcggtc ccaagggtat
     4921 ggctgttcgc catttaaagt ggtacgtgag ctgggtttaa aacgtcgtga gacagtttgg
     4981 tccctatctg cagtgggcgt tggaagtttg acgggggctg ctcctagtac gagaggaccg
     5041 gagtggacga acctctggtg taccggttgt aacgccagtt gcatagccgg gtagctaagt
     5101 tcggaagaga taagcgctga aagcatctaa gcgcgaaact cgcctgaaga tgagacttcc
     5161 cttgcggttt aaccgcacta aagggtcgtt cgagaccagg acgttgatag gtggggtgtg
     5221 gaagcgcggt aacgcgtgaa gctaacccat actaattgcc cgtgaggctt gactctatca
     5281 tttgaaggac ttcaagagat aaaagcttac tgactgattc agtcattacc gaatatattg
     5341 attaaggctt taccgatttg caacagttta agtttggcgg ccatagcgag ttggtcccac
     5401 gccttcccat cccgaacagg accgtgaaac gactcagcgc cgatgatagt gtggttcttc
     5461 catgcgaaag taggtcactg ccaaacaccc tttcagaaaa cccccggata tccgggggtt
     5521 tttgctttgc ccggaaaaaa tgtcggggat ggcgggacgg catctgtacg gtgtccggtc
     5581 gggtttgcgg aggaacggct tgaaactttg ggatattcat tttagaatga cccgttttat
     5641 agcggattaa caaaaatcag gacaaggcgg cgggccgcag gcagtacgga tagtacggaa
     5701 ccggttcgcc cggtgcttca tcaccttagg gaaccgttcc ctttgagccg gggcggggca
     5761 acgccgtacc ggtttttgtt aatccgccat atcgtcgcaa gatgcggttt gttgtttgca
     5821 acccttaaag gaaaaaccat gaaaaaaatg tttgtattgt tctgtatgct gttctcctgc
     5881 gccttctccc ttgcggcggt aaacatcaat gcggcttcgc agcaggagct ggaggcgctg
     5941 ccgggcatag gcccggcgaa ggcgaaggcc attgcggaat accgcgcgca aaacggcgcg
     6001 ttcaagtctg tggacgattt gatcaaggtg aagggcatcg gtccggcggt gctggcgaag
     6061 ctgaaagacc aggcttccgt cggcgcgccc gcaccaaaag gccccgccaa accggtgctg
     6121 cctgcggtta aaaaataggg gaacctgtaa aggaaggggc atcggctgcc gccggcgctt
     6181 ttttgttctt tgtttggaag ggaaatggct ggagaaagac ctgcgctata tcggggaatg
     6241 aatcgatgca aaggaaatgc cgtctgaaca tgcgttcggg cagcgttttt ataacaaaaa
     6301 cacttaatgg cggttggttt tatacctatc taagtttttg tgtcgtgcat atctgaaaag
     6361 tgtattccgt ttcagtttgt aagctgtggc attctgagac ctttgcaaaa aagcccttcc
     6421 ttcgacagcc gaaacccaaa cacaggtttt cagctgtttt cgccccaaat acctcctaat
     6481 tttacccgaa tatc
//
LOCUS       DAWTQS010000085         6269 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.085, whole genome shotgun sequence.
ACCESSION   DAWTQS010000085 DAWTQS010000000
VERSION     DAWTQS010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6269
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.085"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(67..1153)
                     /locus_tag="WNA25_001904"
                     /pseudo
     CDS             complement(67..1153)
                     /locus_tag="WNA25_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951001.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
     gene            complement(1090..1305)
                     /locus_tag="WNA25_001905"
     CDS             complement(1090..1305)
                     /locus_tag="WNA25_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HFA9824590.1"
                     /translation="MSTQFDVAVIGAGPAGSAASALLRKKGYQVCVLENSTFRASSSA
                     KACCRTVWKCWKKPVLPMPFAPGPAFS"
     gene            complement(1485..2858)
                     /locus_tag="WNA25_001906"
     CDS             complement(1485..2858)
                     /locus_tag="WNA25_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFA9824591.1"
                     /translation="MSNPFSSLGLGTELVSALTAQGYENPTPIQAAAIPKALAGHDLL
                     AAAQTGTGKTAAFMLPSLERLKRYATASTSPAMHPVRMLVLTPTRELADQIDQNVQGY
                     IKNLPLRHTVLFGGVNMDKQTADLRAGCEIVVATVGRLLDHVKQKNISLNKAEIVVLD
                     EADRMLDMGFIDDIRKIMQMLPRQRQTLLFSATFSAPIRKLAQDFMNAPETVEVAAQN
                     TTNANVEQHIIAVDTFQKRNLLERLIVDLHMNQVIVFCKTKQSVDRVTRELVRRNLSA
                     QAIHGDRSQQSRLETLNAFKDGSLRVLVATDIAARGLDIAELPFVINYEMPAQPEDYI
                     HRIGRTGRAGADGVAISLMDESEQKMFEAIKELTGNKLLIERIEGFEPRWWEQEGSKP
                     EKTETSEPRQRNRYESARAQREKNTRPETTANDAGAACGKIAGRSRRSRRGHRTCALL
                     QPRYGVK"
     gene            3072..4076
                     /gene="dusB"
                     /locus_tag="WNA25_001907"
     CDS             3072..4076
                     /gene="dusB"
                     /locus_tag="WNA25_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFA9824592.1"
                     /translation="MHIGGYFIDNPIALAPMAGITDKPFRRLCRAFGAGWAVCEMLAS
                     DPTLRNTGKTLHRSDFADEGGIVAVQIAGSDPEQMADAARYNVGLGAQVIDINMGCPA
                     KKVCNVQAGSALMQDEPLVAAILEAVVKAAGVPVTLKTRLGWHDDHQNLPAIAKIAED
                     CGIAALAVHGRARTQMYKGEARYELIAETKSRLNIPVWVNGDITSPQKAAAVLKQTAA
                     DGIMIGRGAQGRPWFFRDLKHYAEHGVLPPALSLAECRAAILNHIRAMHAFYGETVGV
                     RIARKHIGWYIGEMPDGEQARREINRLDNAAAQYDTLAGYLERLAGKTDRWACGYREG
                     "
     gene            4089..4238
                     /locus_tag="WNA25_001908"
     CDS             4089..4238
                     /locus_tag="WNA25_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824593.1"
                     /translation="MPPFGFGRHLSAWFGGRAESRPANVENNKCRLKTSGTGFAKRLS
                     DGIPS"
     gene            4265..4504
                     /locus_tag="WNA25_001909"
     CDS             4265..4504
                     /locus_tag="WNA25_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HFA9824594.1"
                     /translation="MNPATADIAQCIEQNLNQYFKDLNGTEPCGVYDMVLHQVEKPLL
                     VCVMEQCGGNQSKASVMLGLNRNTLRKKLIQHGLL"
     gene            4507..5043
                     /gene="ruvC"
                     /locus_tag="WNA25_001910"
     CDS             4507..5043
                     /gene="ruvC"
                     /locus_tag="WNA25_001910"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFA9824595.1"
                     /translation="MSATVRILGIDPGSRVTGFGIIDVRGRDHFYVASGCIKTPADEP
                     LADRIAVIVRHIGEVVAVYKPQQAAVEQVFVNVNPASTLMLGQARGAALAALVSHKLP
                     VSEYTALQVKQAVVGKGKAAKEQVQHMVVQMLGLSGTPQADAADGLAVALTHALRNHG
                     LAAKLNPSGMQVKRGRFQ"
     gene            5094..5963
                     /locus_tag="WNA25_001911"
     CDS             5094..5963
                     /locus_tag="WNA25_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HFA9824596.1"
                     /translation="MKFIFFVLYVLQFLPFALLHKIAGLIGSLAYLLVKPRRRIGEIN
                     LAKCFPEWDEEKRKTVLKQHFKHMAKLMLEYGLYWYAPAKCLKSLVRYRNKHYLDDAL
                     AAGEKVIILYPHFTAFEMAVYALNQDVPLISMYSHQKNKILDEQILKGRNRYHNVFLI
                     GRTEGLRALVKQFRKSSAPFLYLPDQDFGRNNSVFVDFFGIQTATITGLSRIAALANA
                     KVIPAIPVREADNTVTLQFYPAWKSFPSEDAQADAQRMNRFIEERVREHPEQYFWLHK
                     RFKTRPEGSPDFY"
BASE COUNT         1370 a         1608 c         1858 g         1433 t
ORIGIN      
        1 tgaatgatga aataaaaatg ccgtctgaac ccgattggat tcagacggca ttttgtatgg
       61 caggatttat tcgctttcca actgaccgac ccattctgac aaggcagtca ggcgttgttc
      121 ggatttcgcc acgaacgggt tttcggtatc ccacgcgtag cctgccaaaa tcgaagaaag
      181 catacggctg atttccgggc tgcggtcggg cgcgtacacg acgttttgga agcggaaatc
      241 ataccagccg tccacatagg tgcggaacgc gtctacgccg atcatcaggg gttcggcaaa
      301 ttcggtttgc caatcggcgg cttcgccttt gagttgtttt gtcagcagat cggcagcaag
      361 ttcggcggag tgcagcgcga tggttacgcc cgacgagaac acggggtcga ggaactcggc
      421 ggcattgccc aacagcgcga aatgcctgcc gtgcagtgat ttgacgttgg cggaatagcc
      481 ttggatggag cggaacggaa aatcgttttc ccaaacggct ttgtccaaaa tttcgctcaa
      541 catcgggcat tcgtaaacaa attttttcaa caccgtttcc gattcgccgg caagtttgtc
      601 gggtgtgccg accacgccga cggaacagcg gttgtcgccg aagggaatca gccaaatcca
      661 tacgtcgcgg ttgttgcgga tgggtggtaa tcaggatttt gttgcggtcg aatttcgggt
      721 gggtaatgtt gtcgtcgatg tgcgtgaaat gcgtttggcg cggcggcagg tgcgagggcg
      781 tttccaagtt tagcaggcgc ggcagcacgc gtccgtagcc gcttgcgtcc aagacgaatt
      841 tcgcggtcag ttcatagctc tcgccggtgt cggtttcgat gttcaagcgg gcaaaatcgc
      901 cgctgttgtc gaacgcggtt acgccgtgcc cgaaacgtac ttcaacgcct tgtttggcgg
      961 cttcttcaat caggattttg tcgaacacgg cgcggcggac ttggtaaacc gtgccggggc
     1021 cgtctgaaaa tttatcggtg aaatcaaact cggtatagcg gctgccccac gaaaacgccg
     1081 caccgttttt caactgaaag ccgggcccgg tgcgaacggc atcggcaaaa ccggcttctt
     1141 ccagcatttc catacagtgc ggcagcaggc tttcgccgat gacgaagcgc ggaaagtgct
     1201 gttttccaac acgcagactt gataaccttt tttgcggagc agggcggatg cggccgaacc
     1261 tgccggacct gcgccgatta cggcgacatc gaattgggtg gacattggga aatcctgatg
     1321 gtttgagtgg aaaaggaaat gatattgtac aggcaggaaa tgccgtctga aactgtttgc
     1381 ggaagaaatt tatccggtgc gggcggttgc tcccaatgcg tcagtccggt ttgaaaaatg
     1441 ccgtctgaaa cgggaaatgt tcagacggca tttgattttc agggttattt tacgccgtaa
     1501 cgcggttgga gcagggcgca cgtccggtgt ccccggcggc ttcggcggct gcgcccggca
     1561 atttttccgc aggccgcgcc cgcatcgttt gccgtggttt ccggccgggt atttttttcg
     1621 cgctgagccc tggcggattc gtagcggttg cgttgtctcg gttcgcttgt ttcggttttt
     1681 tccggttttg agccttcctg ttcccaccat cggggctcga agccttcgat gcgttcgatg
     1741 agcagcttgt tgccggtcag ttctttgatg gcctcaaaca ttttctgttc ggattcgtcc
     1801 atcagggaaa tcgccacgcc gtccgcaccg gcgcgccccg tgcgtccgat gcggtggatg
     1861 tagtcttcgg gctgggcggg catttcgtaa ttgatgacga agggcagttc ggcgatgtcc
     1921 agcccacgcg cggcgatatc ggtggcgacg aggacgcgca ggctgccgtc tttgaaggcg
     1981 ttgagtgttt cgagcctgct ttgttgggaa cggtcgccgt gtatcgcctg tgcggacagg
     2041 ttgcggcgca ccagttcgcg cgttacgcgg tcgacgcttt gtttggtttt gcaaaagacg
     2101 ataacttggt tcatatgcag atcgacaatc agccgttcga gcaggttgcg cttttggaag
     2161 gtatcgacgg cgatgatgtg ttgttcgacg ttggcgttgg tggtgttttg ggcggcaacc
     2221 tcgacggttt cgggcgcgtt catgaagtct tgcgccagtt tgcgtatcgg ggcggagaag
     2281 gtggcggaaa agagcagggt ttggcgttgg cggggcagca tctgcatgat tttgcggatg
     2341 tcgtcgataa accccatatc caacatgcgg tctgcttcgt ccagaacgac gatttcggct
     2401 ttgtttaaac tgatgttttt ctgtttcaca tggtcgagca gccgtccgac ggtggcgacg
     2461 actatttcgc agccggcacg caggtcggcg gtttgtttgt ccatgttgac accgccgaag
     2521 aggacggtat gccgcagcgg caggttttta atgtagccct gcacgttttg gtcgatttgg
     2581 tcggcaagtt cgcgcgtcgg ggtcaacacg agcatacgca cggggtgcat tgcaggcgag
     2641 gtgctggcgg tggcgtaacg tttgaggcgt tccagactgg gcagcataaa ggcagcggtt
     2701 ttgcctgtgc cggtttgcgc ggcggcaagc aggtcgtgtc cggccagtgc tttgggaatg
     2761 gccgcggctt ggatgggcgt cgggttttcg taaccttgcg cggtcagtgc ggaaaccagt
     2821 tccgtaccca aacctaaaga ggaaaatgga ttactcatga ttgtagtctt tctttcagac
     2881 cttatgccgt ctgaagcggg aaaccgatag gacggggaaa taaactgccc gcctgtgaac
     2941 ggcggggacg ggaatttgtg tgcggattat gccataaaac ggtgcccgcc ccaatcgcgg
     3001 gcgcgtccgc agattggaaa tcctgcttaa aaaatgtaca atggcgcact tttttgaaac
     3061 gcgggcccat tatgcacatc ggcggttatt ttatcgacaa ccccatcgca cttgcgccga
     3121 tggcgggcat taccgacaaa cccttccgcc gcctctgtcg ggcgtttggc gcaggttggg
     3181 cggtgtgcga aatgctggcc agcgatccga cgctcaggaa taccggaaaa accctgcacc
     3241 gcagtgattt tgccgatgaa ggcggcatcg ttgccgtgca gattgccggc agcgaccccg
     3301 aacagatggc ggatgcggcg cgttacaacg tcggactcgg ggcgcaggtc atcgacatca
     3361 atatgggctg ccccgccaag aaagtgtgca acgtccaagc cggtagcgcg ctgatgcagg
     3421 acgagccgct ggttgccgcc attttggagg cggtggtcaa ggcggcgggc gtacccgtta
     3481 ccctcaaaac ccgtttgggt tggcacgacg accatcaaaa cctgcccgcc atcgccaaaa
     3541 tcgccgaaga ttgcggcatt gccgcccttg ccgtccacgg gcgcgcgcgc acgcaaatgt
     3601 acaaaggcga ggcgcgttac gaactcatcg ccgagaccaa aagccgtctg aacatcccgg
     3661 tctgggtcaa cggcgacatc acttcgccgc aaaaagccgc cgccgtcctc aaacaaaccg
     3721 ccgccgacgg catcatgata gggcgcggcg cgcaaggcag gccgtggttt ttccgcgatt
     3781 tgaagcatta tgccgaacac ggcgttttac cgcctgcctt gagtttggca gaatgcagag
     3841 ccgccatttt gaaccacatc cgcgccatgc acgcgtttta tggtgagacc gtcggtgtgc
     3901 gcatcgcacg caaacacata ggctggtaca tcggcgaaat gcccgacggc gaacaggcgc
     3961 ggcgtgaaat caaccgcttg gacaatgcgg cggcgcaata cgacacactt gccggttatc
     4021 ttgaaaggct tgccggaaaa accgaccgtt gggcgtgcgg ctatcgggaa gggtagggca
     4081 gtattgccat gccgccgttc gggttcggac ggcatctgtc tgcatggttc ggaggtcggg
     4141 cggaatccag gccggcgaat gtcgaaaaca ataaatgccg tctgaaaacg agtggaacgg
     4201 gtttcgccaa aaggctttca gacggcattc cgtcttaaaa acatttcaat caaaaggata
     4261 tgcgatgaac cctgcgactg cggacattgc gcaatgtatc gagcagaact tgaaccaata
     4321 tttcaaagac ctgaacggca ccgaaccttg cggcgtgtac gatatggtac tgcatcaggt
     4381 ggaaaagccg ctgctggtgt gcgtgatgga gcaatgcggc ggcaaccagt ccaaagcctc
     4441 cgtgatgctg ggactgaacc gcaatacctt gcgtaagaaa ctgattcaac acggtttgct
     4501 gtgaatatgt cggcaaccgt ccgtatcttg ggcatcgacc cgggcagccg cgtaacgggt
     4561 ttcggcatca tcgatgtcag ggggcgcgat catttttacg tcgcctccgg ctgcatcaaa
     4621 acgcctgccg atgagcctct ggcagacagg attgccgtga ttgtgcggca tatcggcgaa
     4681 gtcgttgccg tttacaagcc gcaacaggcg gcggtggaac aggtgttcgt caacgtcaat
     4741 ccggcatcga cgctgatgct cggtcaggcg cgcggagcgg cattggcggc tttggtcagc
     4801 cataagctgc ccgtttcgga atacacggcc ttgcaggtca aacaggcggt ggtcggcaaa
     4861 ggcaaggcgg cgaaagaaca ggtgcagcat atggtggtgc aaatgctggg actttcggga
     4921 acgccgcagg cggatgcggc ggacggtctt gccgtcgcgc tgacccacgc cttacgcaac
     4981 cacgggcttg ccgccaaact caatccttcg gggatgcagg tcaagcgcgg aaggtttcaa
     5041 tagtttcaga cggcatttgt attttgccgc ctgaaaagaa aatgtgtacc gagatgaaat
     5101 ttatattttt tgtactgtat gttttgcagt ttctgccgtt tgcgctgctg cacaagattg
     5161 ccggcctgat cggttcgctt gcctaccttc tggtcaaacc gcgccgccgt atcggcgaaa
     5221 tcaatttggc aaaatgtttt cccgaatggg acgaagaaaa gcgtaaaacc gtgttgaaac
     5281 agcatttcaa acacatggca aaactgatgc tcgaatacgg cttatattgg tacgcgcctg
     5341 ccaaatgcct gaaatcgctg gtgcgctacc gcaataagca ttatttggac gacgcgctgg
     5401 cggcggggga aaaagtcatc atcctgtacc cgcactttac cgcgttcgag atggcggtgt
     5461 acgcgcttaa tcaggatgtc ccgctgatca gtatgtattc ccaccaaaaa aacaagatat
     5521 tggacgaaca gattttgaaa ggccgcaacc gctatcacaa cgtcttcctt atcgggcgca
     5581 ccgaagggct gcgcgccctc gtcaaacagt tccgcaaaag cagtgcgccg ttcctgtatc
     5641 tgcccgatca ggatttcgga cgcaacaatt cggtttttgt ggattttttc ggcattcaga
     5701 cggcaacgat taccggcttg agccgcattg ccgcgcttgc aaatgcaaaa gtgatacccg
     5761 ccattcccgt ccgcgaggcg gacaatacgg ttacattgca attctatccc gcttggaaat
     5821 cctttccgag tgaagacgcg caagccgacg cgcaacgtat gaaccgcttt atcgaagaac
     5881 gcgtgcgcga acatccggaa caatatttct ggctgcacaa gcgtttcaaa acccgtccgg
     5941 aaggcagccc cgatttttac tgactacata aaattacaaa acaaatcagg cgtttcagat
     6001 caaaaacccc gattgttttt gggaatttga aacccgggtt gtacaaacag gatttgccgg
     6061 acggttttaa cggttcagtt gtttgtaaaa acaatgcttt tttaaaattg acaaaaaacg
     6121 aaatcggttt taaaggctta ttccgagaac aaaggggagt ggatgccgaa aacccggtta
     6181 atatattata gtggattaac aaaaaccaat acggcgttgc ttcgccttag ctcaaagaga
     6241 acgattccct aaggtgctga agcaccaag
//
LOCUS       DAWTQS010000086         6248 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.086, whole genome shotgun sequence.
ACCESSION   DAWTQS010000086 DAWTQS010000000
VERSION     DAWTQS010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6248
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.086"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(33..723)
                     /locus_tag="WNA25_001912"
                     /pseudo
     CDS             complement(33..723)
                     /locus_tag="WNA25_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
     gene            complement(1425..2267)
                     /locus_tag="WNA25_001913"
     CDS             complement(1425..2267)
                     /locus_tag="WNA25_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFA9824597.1"
                     /translation="MLICNPYEVVIHGTTSSGKIFRPGDWAERLCGILSSFTKDNRLS
                     YSKWVRPMLVDNIRCVAVDKKLETDNPQMFRFLMDFAADNDLRVIDCKALLEEREQGG
                     QNDPANERVLLAQAIEEKHAAEKTQEQTASGASYVLREIGADDTATAFAALSVLRSAL
                     TDIGRFTEQINKTQRPQGYRLLGIFEEGKHNAVAVCGFREACTLAGGRHIHIDDIVTL
                     PQSRRKGYASRLLEEVRKISAETGVTKIHLNVHVNHDRTDAHRLYFKNGFEICAYHFR
                     CDPK"
     gene            2502..3422
                     /gene="pyrB"
                     /locus_tag="WNA25_001914"
     CDS             2502..3422
                     /gene="pyrB"
                     /locus_tag="WNA25_001914"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFA9824598.1"
                     /translation="MPNPLYRQHIISISDLSREQLECLLQTALKLKAHPRGDLLEGKL
                     IGSCFFEPSTRTRLSFETAVQRLGGKVIGFSDGANTSAKKGETLADTARIISGYTDAI
                     VQRHPKDGAARVAAEFSRVPVINAGDGTNQHPSQTLLDLVTIYETQGRLDKLKIAMAG
                     DLKYGRTVHSLCQALKRWGCEFAFVSPPSLAMPDYITEELEEADCRYRALGSLEEAAE
                     WADILYMTRVQRERFDEQEFAKIQGKFNLEASMLARAKPNLRVLHPLPRTDEIHPDVD
                     AAPHAYYFEQATNGVYARMAILSLVLNEEV"
     gene            3432..3890
                     /locus_tag="WNA25_001915"
     CDS             3432..3890
                     /locus_tag="WNA25_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFA9824599.1"
                     /translation="MEAQKLSVEAIEKGTVIDHIPAGRGLTILRQFKLLHYGNAVTVG
                     FNLPSKTQGSKDIIKIKGVCLDDKAADRLALFAPEAVVNTIDNFKVVQKRHLTLPDEI
                     AEVFRCPNPNCAGHGEPVKSRFYVKKHNGQTRLKCHYCEKTYNRDSVAEA"
     gene            3952..4617
                     /locus_tag="WNA25_001916"
                     /pseudo
     CDS             3952..4617
                     /locus_tag="WNA25_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273228.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5022..6245)
                     /locus_tag="WNA25_001917"
     CDS             complement(5022..6245)
                     /locus_tag="WNA25_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFA9824600.1"
                     /translation="MQRRIITLLCAAGMAFSTQTLAANLEVRPDAPQRYTVKQGDTLW
                     DISGKYLYSPWQWGSLWGANRDQIHNPDLIYPGQVLVLRYVGGEPRLDLEQTDGIPVV
                     KMSPDKEVSGYGIPAVNANFYRIFIRHPQIVSRKETAAAPRLLSGPEGRLLYTKGARV
                     YTKGLKEPGRYLTYRINKNITDPDTGKFLGQEVAFSGIVRSLDYTDSALEQRSKQAEE
                     RLKDNEYYTRTHPLITPVRTRSIQPLVVETAISEIQQGDYLMKMPEDTDRFNMVPHEP
                     SRPVQAKIVSVFEGVGVGGQFKTITIDKGGDDGLDKGAVLSLYKRKKTMQVNLSNNLT
                     EEPKSRDTVELISTPAEEVGLAMVYHTAPKLAYAIILENISDISEGDTAANPGRDLDN
                     MPDQGRARVDSDPFQ"
BASE COUNT         1492 a         1522 c         1749 g         1485 t
ORIGIN      
        1 ctatataaaa taaatgggca aaaatcggtt tattatcgtt tttgccgcat ttggatttgt
       61 tctaccgtaa aacgtgtttg acgaacggga tttttattaa aaaacatctg atttctaaca
      121 aaatcagtat tttttggcac gatggctaaa atttttcctt ccatttcgcc atcacgtgtt
      181 ttccatgcgc tcaagaattg tgatttgctc gttgagacgt gccccagcga tggatcagcc
      241 agcaaaacag tttctccgtt aataccgttc aataccgaaa aatggttgtt tttacggtat
      301 tttaaataca caattacagg aatttttagt tgtaccaact gttcaaatgg caaagcataa
      361 ccttgtgctt caaaacccag ttcgggcatt atgcgttgca tatcatcaaa agaagcacgc
      421 atttgggttt tatccatttt gtctaagatt tccgcttcag aataatgtct gccataaaaa
      481 ttattcagta acgtggcaat cgaagccgcg ccgcaagaaa aatccaaatc ttgttttact
      541 atgccggaat ctcgccgtgc tttccaactc cgtacatgga tgttttggta agaagcgggg
      601 gtcaacaaac ataggccaag caaaaactat atttggggcg aaaccaatca aagccgcata
      661 atttatcaat ttataaagat tttttatcat aatatgtata cgcggaataa acgcatgaaa
      721 cataaaaaaa taaaatcata tgcaatttta ttgcatgaat aaatatgaat aaaatagata
      781 atgatgggag taaatacgcc atgtattttg gaagtttaaa tttattaata ataaaataat
      841 ttatcgtagc gcaaataaat cccaaaattg gaacgattag aaaaaaaatt aatacaccca
      901 taatcatgca acctatatta ataaatagct aactaattct attacagaaa ttagttagct
      961 gattaaaagt gattaacaat tatagtggat taacaaaaat caggacaagg cgacgaagcc
     1021 gcagacagta caaatagtac gaaaccgatt cacttggtgc ttcagcacct tagggaatcg
     1081 ttctctttga gctaaggcgg ggcaacgccg tactggtttt tgttaatcca ctataaatag
     1141 cgagcatcga ttcacggtgc gctttagtgc aaagcgtaca acgcgagcct gaaccaccag
     1201 ccgcaatagg aaaagaaagc cgatagagtg caatgcttgc caacgcgcaa gcgagcttgc
     1261 aagaacgctt ggctcaacga gagcaggcaa gacagaaagc aggataggag aggcaacgca
     1321 aaggtctcgg gctttgattt cgccgtaaac ctgagtgccg ccttgtccgg acggggtggt
     1381 tcaggcggcg agtgccgaca gggtgcagat gaggaggagt attttcattt ggggtcgcaa
     1441 cggaagtggt aggcgcagat ttcaaaaccg tttttgaaat acaggcggtg cgcgtcggta
     1501 cggtcgtggt tgacgtggac gttaaggtgg attttggtaa cccctgtttc cgcgctgatt
     1561 ttgcggactt cttccaaaag gcgcgaggcg tagcctttgc ggcggctttg cggcagggta
     1621 acgatgtcgt cgatgtggat gtggcggccg ccggcgaggg tgcaggcttc gcggaagccg
     1681 cagacagcga cggcgttgtg tttgccttct tcaaaaatgc ccagcaggcg gtagccttgg
     1741 gggcgttggg ttttgttgat ctgttcggta aagcggccga tgtcggtcag ggcggaacgc
     1801 aaaacactca aggctgcaaa ggcggtggcg gtgtcgtccg cgccgatttc gcgtaaaacg
     1861 taggatgcgc ccgaggcggt ctgttcctgt gttttctcgg cggcgtgttt ttcttcgatt
     1921 gcctgtgcca gcaggacgcg ttcgttggca gggtcgtttt gtccgccctg ttcgcgttct
     1981 tcgagcaggg ctttgcagtc gatgacgcgc aggtcgttgt cggcggcaaa gtccatcagg
     2041 aagcggaaca tctgcggatt gtcggtttcc agttttttat cgacggcgac gcagcggatg
     2101 ttgtccacca gcatggggcg cacccatttc gaataggaga ggcggttgtc tttggtgaac
     2161 gaggacagga tgccgcacag gcgttccgcc cagtcgccgg ggcggaaaat cttgccggaa
     2221 ctcgttgtgc cgtggatgac gacttcgtag gggttgcaga ttaacatggc ggcttcctga
     2281 aaagaaatgt ctggcgcgat tataccttat gcttatgcgg gcgtgtttgg atatgccgtc
     2341 tgaaaaatac aggattcgtg cggtaaaact ttgtggaggc aaatgtgcga taatacgcgc
     2401 cgtgtcgccg cttttgcgaa gctgttccgc aaacatacgg gcggcgtgga cgtataaccg
     2461 gatgcccgcc tgacgcgggt tttttacgga aggggggcaa aatgcctaat ccgctttaca
     2521 gacagcacat catctccatt tcggatttgt cgcgcgaaca gttggaatgc ctgcttcaga
     2581 cggcattgaa gctgaaggcg catccgcgcg gcgatttgtt ggaaggcaaa ctcatcggtt
     2641 cgtgcttttt cgagccgtcc acgcgcacga ggctgtcgtt tgaaacggcg gtgcagcgtt
     2701 tgggcggtaa ggtcatcggc ttttcggacg gcgcgaatac cagtgccaaa aaaggcgaaa
     2761 cgcttgccga taccgcccgt atcatttccg gctacaccga cgcgattgtc caacgccatc
     2821 ccaaagacgg cgcggcgcgc gtggcggcgg agttttcgcg cgtccccgtc atcaacgccg
     2881 gcgacggcac gaaccagcac cccagccaaa ccttgttgga cctggtaacg atttatgaaa
     2941 cgcaggggcg tttggacaag cttaaaatcg ccatggcggg cgacttgaaa tacgggcgca
     3001 ccgtgcattc gctgtgtcag gcgttgaaac gctggggctg cgaatttgcc ttcgtctcgc
     3061 cgcccagcct cgcgatgccc gactacatta ccgaagagtt ggaagaagcc gactgccgat
     3121 accgcgccct cggcagtttg gaagaggcgg cggaatgggc ggacatcctg tatatgaccc
     3181 gcgtccagcg cgaacgtttc gacgaacagg aatttgccaa aatccaaggc aaattcaacc
     3241 tcgaagcgtc tatgctcgcc cgcgccaaac cgaacctgcg cgtgctgcac cccctgccgc
     3301 gcacggacga aatccacccc gatgtcgatg ccgcgccgca cgcctattat ttcgagcagg
     3361 cgaccaacgg cgtttatgcg cgtatggcga tattgtcgct ggtgttgaac gaagaagtgt
     3421 gaggaaccga tatggaagcg caaaaactca gtgtcgaagc catcgaaaaa ggcacggtta
     3481 tcgaccatat tcccgccggc agggggctga ccatcctgcg ccagttcaaa cttttgcact
     3541 acggcaacgc ggtaaccgtg ggcttcaacc tgcccagcaa aacccagggc agcaaagaca
     3601 tcatcaaaat caaaggcgtg tgcttggacg ataaagccgc cgaccgcctc gccctgttcg
     3661 cccccgaagc ggtggtcaac accatcgaca atttcaaggt cgtacagaag cggcatttga
     3721 ccctgcccga cgaaatcgcc gaagtgttcc gctgcccgaa tcccaattgc gccggccacg
     3781 gcgagccggt caaaagccgg ttttatgtta agaaacacaa cgggcagacg cggctgaaat
     3841 gccactactg cgaaaaaacc tacaaccggg attcggtggc ggaagcctga cggattcccg
     3901 agcccggcgg cggaattttg cctgccgcct gttttgccaa tctgaaatgg aatgatgatg
     3961 cacgcttctg tccaaagtcg tttcgcaccg atactttatg ttttgatttt ctttgccggt
     4021 tttttgaccg cgcaaatctg gttcaatcag aaagcctata ctgaagagct gcctccgctt
     4081 ctgtccgcat tgtccgccgt cgcgctggtg tggctggcgt gggcgttcgt gtcggtgcgt
     4141 tcaaaggcta aggcagaaaa gttctaccgc gaaaaaatga tacagaacga aagcataccc
     4201 ccgtcctgca cgcttctttg caacacttgg aacacaagcc gcaaatgctc gccctgctgg
     4261 tcaaaaacca cggcaaaggc atggcggaac aggtcaggtt caaggcggaa gtgctgcccg
     4321 acgacgaaga cgcgcgcacg attgccgccg agttggcaaa aatggatatg ttcgcattgg
     4381 ggacggacgc ggtcgcctcg ggcgaaacct atgggcgcgt gttcgccgat attttcgagt
     4441 tgtcggcggc tttggaaggg cgcgcgttca aagggatact gaaactgacg gcggaatata
     4501 aaaaacatct tcggcgatgc ctgccgttcg gaaacggcgt tggatttggg cgcgctcaat
     4561 caggcgttga gggaaatctc gaaaacgccg gaaaagccta agcagatatt ttattgaagg
     4621 tgtcccgatg tatggggagc gcacgggtgg cgcggcggca aagcggcaag attgggtttg
     4681 gatacccggt tggttttata gtagattaac aaaaatcagg acaaggcggc gggccgcaga
     4741 cagtacaaat agtacggaac cgattcgccc ggtgcttgga caccttaggg aatcgttccc
     4801 tttgagccgg ggcggggcaa cgccgtaccg gtttttgtta attcgctata tctgtctgca
     4861 agggagacag ccgctgtgat ttccgttcgg gcggaaaccg attccatcgg ataaaaggca
     4921 ttttgtccga ctaaatcagg gttttgcatc aacgggatat gaaaacacag cctaaataaa
     4981 aatgccgtct gaatgctcca gacggcattg ttgccgaggg gttattggaa agggtcggaa
     5041 tcgacgcggg cgcggccctg atccggcata ttgtccaaat cccgtcccgg attggcggcg
     5101 gtgtcgcctt cggaaatatc ggagatgttt tccaaaataa tggcgtaagc cagtttcggc
     5161 gcagtgtggt aaaccattgc caagcccact tcttcggcag gtgtcgaaat cagctcgacg
     5221 gtatccctgc ttttaggttc ttcggtcaaa ttgttggaga ggttgacctg catcgttttc
     5281 ttgcgtttgt aaaggcttaa aactgcgcct ttgtccaaac cgtcgtcccc gcctttgtct
     5341 atcgtaatgg ttttgaactg tccgccgacc cccacgcctt caaacacgga aacgattttt
     5401 gcctgaaccg ggcgggacgg ttcgtgcggc accatattga agcggtcggt gtcttccggc
     5461 attttcatca ggtagtcgcc ctgctgtatt tcggaaatgg cggtttcgac caccagcggc
     5521 tgtatcgaac gggtgcgcac cggggtaatc aaaggatggg tgcgggtgta gtattcgttg
     5581 tctttcagcc gttcttcagc ctgtttcgag cgttgttcga gggcggagtc ggtatagtcg
     5641 agggagcgca cgatgccgct gaatgcgact tcttgtccga ggaatttacc cgtatccgga
     5701 tcggtgatgt ttttattgat gcggtaggtc aggtagcggc ccggctcttt caggcctttg
     5761 gtgtaaaccc tggcgccttt ggtgtacagc agcctgcctt ccgggcccga gagcaggcgc
     5821 ggcgcggcag cggtttcttt gcgggaaacg atttgcgggt gccgtataaa gatgcggtag
     5881 aagttggcat tgacggcagg gatgccgtat ccggacactt ctttatccgg actcattttg
     5941 acgacgggga tgccgtctgt ctgttccaaa tcaaggcgcg gttcgccgcc aacgtagcgc
     6001 aacaccaata cctggcccgg ataaatcagg tcgggattgt ggatttgatc ccggttcgcg
     6061 ccccacaggc tgccccattg ccacgggctg tacaggtatt tgccggagat gtcccacagg
     6121 gtgtcgccct gtttgaccgt gtagcgttgc ggcgcatccg ggcgcacctc cagatttgcc
     6181 gccaaagttt gtgttgagaa tgccatgcct gccgcgcaga gcagggttat aatacgacgt
     6241 tgcataac
//
LOCUS       DAWTQS010000087         6234 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.087, whole genome shotgun sequence.
ACCESSION   DAWTQS010000087 DAWTQS010000000
VERSION     DAWTQS010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6234
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.087"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(208..1593)
                     /locus_tag="WNA25_001918"
                     /pseudo
     CDS             complement(208..1593)
                     /locus_tag="WNA25_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238907.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
     gene            1711..1787
                     /locus_tag="WNA25_001919"
     tRNA            1711..1787
                     /locus_tag="WNA25_001919"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1745..1747,aa:Arg,seq:acg)
     gene            1805..1879
                     /locus_tag="WNA25_001920"
     tRNA            1805..1879
                     /locus_tag="WNA25_001920"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1838..1840,aa:Glu,seq:ttc)
     gene            2042..2158
                     /locus_tag="WNA25_001921"
                     /pseudo
     CDS             2042..2158
                     /locus_tag="WNA25_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            2316..3491
                     /locus_tag="WNA25_001922"
     CDS             2316..3491
                     /locus_tag="WNA25_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HFA9824601.1"
                     /translation="MQNIFDPLIIRGKSLIPIVQGGMGVGVSASGLSSAVARENGIGT
                     IASVDLRHLHEDLLAESQINPSEEKYTSLNCTALDREIQKAKSASEGKGLIAVNVMKA
                     VKDHAAYVRQACESGADAVVMGAGLPLDLPEMTEGYHKDVALLPILSESRGINIVLKR
                     WMKKGILPDAIVVEHPAHAAGHLGASTVEGVNDAKFDFKRVIEETFEVFKNLGLEGEK
                     IPLILAGGMANFEKVKTALKNWGASAVQIGTAFAVTEEGDAHLNFKKTLASAETEKVV
                     EFMSVAGLPARGIRTKFLDSYIRREGKLQANAKADPRRCTQGLNCLTSCGLRDGLSKA
                     GQFCIDIQLAAAFRGEVDKGLFFRGKDQLPFGNAIRTVRETIQYLLTGSEPVATLGR"
     gene            complement(3833..4381)
                     /locus_tag="WNA25_001923"
     CDS             complement(3833..4381)
                     /locus_tag="WNA25_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HFA9824602.1"
                     /translation="MSQNNPIKPWYKHVWPWVLMAGPIFVVIASVAMFFVAQQHATDL
                     VTDDYYKDGKHIDIQLHRDEEAVRRHIGVQVLISPDMNAAKVFVGGEFDGKQPLNLLL
                     MHPTRKADDQTVALKPVGSAQNGRAEYEAVFKTLPPANHWYVRVEDAAGVWRVENKWI
                     TSQGNAVDLTPMDKLFNNAGSK"
     gene            complement(4384..5880)
                     /gene="ccoG"
                     /locus_tag="WNA25_001924"
     CDS             complement(4384..5880)
                     /gene="ccoG"
                     /locus_tag="WNA25_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HFA9824603.1"
                     /translation="MTTENQAGSPASGIGTSEQTKAAPKVKKTFDPRASVIQIHPEGE
                     RIHPKKAEGRFAKLRIAAVLATQFVFYVIPWFNWSGRQAVVFNIPERHFFIFGLSLGV
                     GDLIYLALLLMICAFGLFWWTTIAGRLWCGYSCPQTVYTEIMLWIDNLVEGDRNKRLK
                     LEKSPWNFTKIRIKATKYLLIFLVCAWTGITFAGWFVPIRQFVPDLFTGAAGGGAMFA
                     AAFYGFMTFFFAHIMREKVCLHMCPYARFQSAMFDKDTLIVSYDAERGEPRGARKKTV
                     NKEEAGLGDCINCAMCVQVCPVGIDIRNGLQYQCIGCAACIDACDEIMDKMGYPSGLI
                     RYTTESALEHEYAEKDIKKRLLRPRVAGYGAVLAVVVAAFLVGLSTRKMVEVDILKDR
                     GVMVRENAKGWLENAYSLRIINNSEKEQLITASVKGFDEIALTGLPEGGIKVAPRETI
                     TLPVQVSTIPEYADKGSHPIEFIFQYRESGASDGKPVVLEEDATFIGE"
BASE COUNT         1620 a         1690 c         1421 g         1503 t
ORIGIN      
        1 ccaccctgct tgtccgatgc ctcgggacgg acgggcaaac cgacacggca cgcaaaccgc
       61 gtcggcaaaa atgccgtctg aaaaaattca gacggcatct tcagacacat tacctgcaaa
      121 cggcaacaca ttccaatcca aaccgatcag gcaatcaata cgaacggctg tttacatact
      181 taccagcttt caccaaccgg tatcgattta accgatttcc ttaatatttt tcctgtccgt
      241 tttaaacttc gccttaaaca catccggtaa atctttatcg aaataccaaa gcccgtcatc
      301 catttccaat gcgccgccca ttccatgcag aacgactttt cccccgttaa aggatcggtt
      361 tcggtaattt ccacctcatg catttttccc caatccaaaa caggcaactt gtgtgcaaac
      421 gccaaaactt gttcgtcaaa acggccgcac gccttatcgc atcggcctga aacatatacg
      481 ggacaaggct catcctctgc ataaccgtcc ggaaggatac cggctgaggc aggacacaat
      541 ccgtatgttt ccgcccacga cgacaaagcc cgtctcgccg cctttttatt ggcaaataat
      601 ccggtaggcg gattatccgt cacaccgttt ttcaaagccg ctgttttcgc attcaacatg
      661 ccgtctgaac ctttttcaaa cctgacggtc gtaaatgttt taagcagatt tttggcagat
      721 acataacaac ccgaatgata acgtccgacc aattccgctt taatcttata tgcatgcagg
      781 ctgcccaatg cgggaaaaaa acggacttcc tccgtattgc accaatcaaa cgggggcttt
      841 tccggaatcc aacaaagccg caacctcgcc atatgcccgt tcaaacgtac cgatataatt
      901 tacttttccc ccgccgtcga aacaggcaag cacacctgta ccgtcgggca aaccgtacag
      961 ccgttccctc aaccgttcgg gaagcgcggc aggaagaagt ttcggattcg tcaaacggaa
     1021 acactgcctg ctccactcct caagcccgtg tgccgacagg ctgtattcca aataatcaca
     1081 caacgccgat acatccgcca tcgcacgatg cctgtcttcc acaacaatcc ccaacctttc
     1141 gatgataccg tccaggctgt gcttgtaaaa ctgcggatac agccgacgcg aaagctgcac
     1201 actgcacaaa gcaggcgatg aaaatccgat acccgcacga tgaaactcat gctttaaaaa
     1261 cgtatagtcg aaacggctgt tgtgcgccac cagcacgcac cccttcaata ccgaaaacaa
     1321 cccgccggca atttcagcaa acaccggcgc gtcggcaacc atgccgtctg aaatccccgt
     1381 cagttccgcc acaaacttcg gaatcggttt ttgaggattg accagccatt catacctctc
     1441 cgcccttccc tgcccaaact tgaccaaagc cacttcggtt accctgtctt catacagatt
     1501 gccgcccgtc gattccaaat caaccacggc aacaggcatt ccaaaccgta aaaacgcctt
     1561 ttccagcaag ggccagcggg aaacaacaat cattttattc tctttaaatt caaacaacaa
     1621 accgatattt tacactttta aggcatttca tccaacaaaa taattgacag aatccgatga
     1681 ttaccataaa attcaaaaat ttattgcagc gcacccgtag ctcagttgga tagagtatct
     1741 ggctacgaac cagagggtcg ggcgttcgaa tcgctccggg tgcgccagta agaaaataca
     1801 atacgcgccc atcgtctagc ggttaggaca tcgccctttc acggcggtaa ccggggttcg
     1861 attccccgtg ggcgtgccag attccaaatc cccgaaataa tccctcgggg atttttattg
     1921 cctcggcaat tcgttaccat atctttacct acccacttca tcagaatctc agacgtaatc
     1981 gaatcatatt caaacttttg ccatgcaaac cgatatccca taaccggatg cggtgtccgt
     2041 tcaatatagt ggattaaatt taaatcagga caatgcgacg aagccccaga cagtacaaat
     2101 agtacggcaa ggcgaggcaa cgccgtaccg gtttaaattt aatccactat attttgtccg
     2161 attggtacag atataacagc atattccaaa gctccttctg cttttctgtt taccactgca
     2221 atatttagag tataatacca aatttgagta atggttctaa aacagttaaa accatttttc
     2281 atgagcctga ctgattcgta tattcggaga aactgatgca gaatattttt gaccctttga
     2341 ttattcgtgg aaaatccctt attcccatcg tgcaaggcgg tatgggggtc ggtgtttccg
     2401 catcgggttt atccagcgcg gtggcgcgtg aaaacggcat cggaacgatt gccagtgtag
     2461 atttgcgcca ccttcacgaa gacctgctcg ccgaatccca aatcaatccg agtgaagaga
     2521 aatatacgtc tttgaactgt accgcattag acagggaaat ccaaaaagcc aaaagtgctt
     2581 cagagggaaa aggactgatt gcggtcaacg tgatgaaggc ggtcaaagac cacgccgcgt
     2641 atgtccgcca ggcttgcgaa tcaggggcgg atgcggtcgt gatgggtgcc ggcctgcctt
     2701 tagacctgcc ggaaatgacc gagggctatc ataaggatgt cgcgctgctg ccgattctgt
     2761 ccgaatcgcg cggtattaat atcgtcttga aacgttggat gaaaaaaggc atattgcccg
     2821 atgcgattgt agtcgaacat cctgcccatg cggccggaca tttgggtgca tcaaccgttg
     2881 aaggcgtaaa cgatgccaaa ttcgacttca aacgcgtgat tgaggaaacg tttgaagtgt
     2941 ttaaaaattt ggggctggag ggagaaaaaa tcccgcttat tcttgcggga ggcatggcaa
     3001 attttgaaaa agtcaaaacc gccctaaaga actggggagc ctccgccgtt caaatcggta
     3061 cggcttttgc cgttaccgaa gaaggagatg cacacctcaa ctttaaaaaa acgctcgcca
     3121 gtgcggaaac tgaaaaagta gtcgaattta tgtctgttgc cggtttgccg gcgcgcggta
     3181 tccgcaccaa attcctagac agctatatca ggcgcgaagg caaacttcag gcaaacgcca
     3241 aagccgaccc gcgccgctgt acccaaggtt taaactgcct gaccagctgc ggtctgcgcg
     3301 acgggctttc caaagcaggg caattctgta ttgatatcca gcttgctgcc gcattccgtg
     3361 gagaagttga taaggggttg tttttcaggg gaaaagacca gcttcccttc ggtaatgcca
     3421 tccgcaccgt ccgcgaaacg atacaatatc tgctgacggg gagcgaacct gttgcaacgc
     3481 tcggacgctg acatcaggta ggatttgatt tgcaaacaaa atgccgtccg aagggctttc
     3541 agacggcgtt tttcaggttg tgttccggaa cagtgtcgaa aaaacggaga gaaataaaaa
     3601 cgtttttatg tgaacaaaat gccttccgca ttatgtcgga catacaacac aaatgccgtc
     3661 tgaagccttt cagacggcat ttttcaggct gcctttcgga atgatgttaa aaaataaacg
     3721 ggatgagata catttatttt cgtccgacaa atcagaccat cacgccgaat aatcaaacaa
     3781 tgcctgcccg acattgcata taacaaacca atgcaatgaa aacacggctt ttttatttgc
     3841 ttcctgcatt attgaaaagt ttgtccatcg gggtcaaatc gaccgcattg ccctggctgg
     3901 taatccattt gttctcgacg cgccacacgc ctgccgcgtc ctccacgcgc acataccagt
     3961 ggttggccgg cggaagggtt ttgaacaccg cctcatattc cgccctgccg ttctgcgcgc
     4021 tgccgacggg cttgagggcg acggtttgat cgtccgcctt gcgggtcggg tgcatcagca
     4081 gcaggttcaa aggctgtttg ccgtcaaact cgccgccgac aaacactttt gccgcattca
     4141 tatcgggaga aatgaggacc tgcaccccga tatgccgtct gacggcttct tcatcccgat
     4201 gaagctggat gtcgatatgc ttgccatcct tataataatc gtccgtaacc aaatctgtcg
     4261 cgtgctgctg cgcgacaaaa aacatagcga cgctggcgat gacgacaaaa atcggccccg
     4321 ccatcaagac ccacggccag acgtgtttgt accaaggctt gattggattg ttttgagaca
     4381 cggttattct ccgataaagg ttgcgtcttc ttccaaaaca accggcttgc cgtctgatgc
     4441 accgctttcc cggtattgga agataaactc gataggatga ctgcctttgt ccgcgtattc
     4501 cggaatggtg gacacttgga cgggaagggt tattgtttcg cgcggagcca cttttatacc
     4561 gccttcaggc agcccggtca gggcgatttc gtcaaatcct ttgacacttg cggtaatcag
     4621 ctgttctttt tcgctgttgt tgatgatacg caggctatat gcgttttcca gccaaccttt
     4681 ggcgttttcg cgcaccatta cgccacggtc tttcagaata tcgacttcga ccattttgcg
     4741 cgtggacaaa ccgaccagga aggcggcgac aaccactgcc aacaccgcgc cgtaacctgc
     4801 cacgcgcggt ctgagcagcc gttttttaat gtctttttcg gcatattcgt gttccagcgc
     4861 gctttcggtc gtatagcgta tcaaaccgct cggataaccc attttgtcca taatctcatc
     4921 gcacgcgtcg atacaggcgg cgcagccgat acattggtat tgcaggccgt tgcggatgtc
     4981 gatgccgacg gggcagactt ggacgcacat cgcacagttg atgcagtcgc ccaaacccgc
     5041 ctcttccttg ttgaccgttt tcttgcgggc gccgcgcggt tcgccgcgct ccgcgtcata
     5101 agaaacaatc agcgtgtcct tatcgaacat cgcgctttgg aaacgtgcgt acggacacat
     5161 atgcaggcac actttttcgc gcataatatg ggcgaagaag aaggtcataa agccgtaaaa
     5221 cgctgcggca aacatcgcgc cgccgcctgc tgctccggtg aataaatcgg gaacaaactg
     5281 gcggatgggg acaaaccagc cggcaaacgt gatgcccgtc cacgcgcaga caaggaaaat
     5341 caacaggtat ttggtggctt tgatgcggat tttggtgaaa ttccacggcg atttttccag
     5401 cttcaggcgt ttgtttctat cgccttcgac caggttgtcg atccacagca taatttcggt
     5461 gtaaaccgtt tgcgggcagg aatagccgca ccacaaacgg ccggcaatcg tcgtccacca
     5521 aaacagcccg aaggcgcaaa tcatcagcag cagggcaagg taaatcaaat cgcccacccc
     5581 caacgacaat ccgaaaatga agaaatgccg ttcggggata ttgaaaacga cggcctgcct
     5641 tccgctccaa ttgaaccacg gaatgacgta aaacacaaac tgggtcgcca atacggcggc
     5701 gatacgcagt ttggcgaacc gtccttccgc ctttttggga tggatgcgtt cgccttcggg
     5761 atggatttga atcacgctgg cacgcggatc gaacgttttt ttcactttcg gcgcggcttt
     5821 tgtttgttcg gacgtgccga ttccggatgc cggactgccg gcttggtttt ccgtggtcat
     5881 tctgcattcc ttagattttt gattgatggt ttgcccgtta ccgccgccgt tttgcttttc
     5941 agacgtcatt tttcttgttt ttttaaggca ttgtgtttca agttttgaga aaatccgctt
     6001 ttcccaaaat atattcccgc tattgtacaa ctttatgcgc cgtccggatg tattgggcga
     6061 atacatttcc catccgcatc aaaacgccct gattttaccg caccgcccga acaaaattaa
     6121 aatactctta aaaaacaaag actaaaaaaa acagacaccc cttatcggca gaaccgccgg
     6181 cgcaagctca taaacaaacg ctatcaacaa tccggcacac aatctataac gttt
//
LOCUS       DAWTQS010000088         5714 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.088, whole genome shotgun sequence.
ACCESSION   DAWTQS010000088 DAWTQS010000000
VERSION     DAWTQS010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5714
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.088"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(<1..814)
                     /locus_tag="WNA25_001925"
     CDS             complement(<1..814)
                     /locus_tag="WNA25_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLxRFG domain-containing protein"
                     /protein_id="HFA9824604.1"
                     /translation="MSDLVRYDPLEHGRLAGGLKEYRGFTQKDARAAADDTALTRGFK
                     NSMRSARMGWNALTGDKEELGRLKAEDMDYRKIQEGRKSQARRELGEAWEKGGGVGGG
                     LSNVWGELKKDWREKGLDGALEDVGEMGGAVLEQAPNALVPIATTTAGGILGALAGGN
                     AAVGAYAGATLGNTLMEYGGQLDRAAEAAGVDPADKDAVMAFIGRGAPGALKNAAVKG
                     AVVGAADMAAMKLGGSILNMGKKAAGKAALEKMGVAAADKAAVAAAKGTPEFA"
     gene            complement(811..2007)
                     /locus_tag="WNA25_001926"
     CDS             complement(811..2007)
                     /locus_tag="WNA25_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824605.1"
                     /translation="MGLFEPSAGDFWEMKEKEKKEKARKGAEERERAAAQAHRADAVR
                     RTVANYEAGPARYRNVMDLSRNNIEDGARRLRRAGAFERGADAGLGFSGGDKALSPDA
                     RAGADFARRDTRPTDAGGRTPPPLGFDGNVYRDGKPVRDFDAQRPLVSAGPDALSPEE
                     RELYRRATTPHAGALNGQLTAAQLNAARGIVAEHNKNAAVRELGRERLAAAAAENAAN
                     REAVLQEGRFDAAVKANEGALNREMAQRNADRAFDVQQAELGMKRQGFEMKREADALE
                     LEDRKRIADLTRAYGFAKSDGQRGEIARQIDALNGKFERQGEKGFDPNVFKTISYEVA
                     DPDTGLTAKREGIVDLRTGKPLDVEFAGEREKRYAQLGFKPNGQKTAGGKIIYENEKG
                     EKRVEQ"
     gene            complement(2244..2432)
                     /locus_tag="WNA25_001927"
                     /pseudo
     CDS             complement(2244..2432)
                     /locus_tag="WNA25_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503496.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2418..3455)
                     /locus_tag="WNA25_001928"
                     /pseudo
     CDS             complement(2418..3455)
                     /locus_tag="WNA25_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701186.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
     gene            complement(3436..3678)
                     /locus_tag="WNA25_001929"
     CDS             complement(3436..3678)
                     /locus_tag="WNA25_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824606.1"
                     /translation="MAENLSKSAEQIVAELYGSPEDTFTKARMFQTVAAGFKVLVEIE
                     RKSYGMDTAESKISESAKTAGIRIEFVGPEDDGKDG"
     gene            complement(3736..3975)
                     /locus_tag="WNA25_001930"
                     /pseudo
     CDS             complement(3736..3975)
                     /locus_tag="WNA25_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706439.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3975..4397)
                     /locus_tag="WNA25_001931"
     CDS             complement(3975..4397)
                     /locus_tag="WNA25_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824607.1"
                     /translation="MSGLSDIDKVYQGVVDLHNAEQVVSREALAETTGLKMSEITKFT
                     KLLVEHGKIYRVTRGIFKPAIGFGETRPVSVSVLDSGMGVLEIGDTVLHLNPQEMRSL
                     GALMSGFGQQFSSIQMGREFSVLRNYLECSAKNGRLDL"
     gene            complement(4462..4980)
                     /locus_tag="WNA25_001932"
     CDS             complement(4462..4980)
                     /locus_tag="WNA25_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFA9824608.1"
                     /translation="MGLTQEVLKELLRYDDNTGKLYWAERPRKYFNSGLHYKSWNTGF
                     SGKEVFLYKGRLGYLKLKIFKKQYNAHRLIWLFVYGKHASSIDHINRDKTDNRISNLR
                     DVTHAENMKNRGKFKNNTSGHTGVYFHKPSKKWQARIMVNRKNKILGLFEHIEDAVKA
                     REAASKDFGFVV"
     gene            complement(4971..5354)
                     /locus_tag="WNA25_001933"
     CDS             complement(4971..5354)
                     /locus_tag="WNA25_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFA9824609.1"
                     /translation="MNGAEFTLTPQNKKQVMRSIWDSPDGWFENGNLEITIRPRKSKR
                     SVEQNRRLWFLYREISEKVFIDGRRFSQDVWHEFLKRKFIGCIEMPNGQLMGISTTKL
                     SVREMSEYQEKIISWASMEHGVLWD"
     gene            complement(5351..5701)
                     /locus_tag="WNA25_001934"
     CDS             complement(5351..5701)
                     /locus_tag="WNA25_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 family protein"
                     /protein_id="HFA9824610.1"
                     /translation="MRQTRVGISSDRRVGMSSAIRKAAKGGQCTPNIAGVCNDNPETV
                     VLCRFPGETHGAGLKSGGLGAGFGCGCRRGAIDGRGAGLSREDKEFYMRRSQLRTIRR
                     LEALGVVGVKGRLK"
BASE COUNT         1219 a         1967 c         1184 g         1344 t
ORIGIN      
        1 ccgcaaattc aggcgttccc ttagccgccg caaccgccgc cttatccgcc gccgcaacac
       61 ccattttctc caatgcggct ttcccggcgg ccttcttgcc catattcaaa atactgccgc
      121 caagtttcat cgccgccata tccgccgcgc cgaccaccgc gcccttgacc gccgcatttt
      181 tcaacgcacc cggcgcacct cggccgataa acgccatcac cgcatccttg tccgcagggt
      241 cgacgcccgc cgcctctgcc gccctgtcca gctgcccgcc gtattccatc agcgtattgc
      301 ccaaggtcgc gcccgcatag gcgccgacag ccgcgttacc gcccgccaaa gcgcccaata
      361 tgccgccggc ggttgtcgta gcaataggga caagcgcatt gggcgcctgc tccagcaccg
      421 cgccccccat ctcgcccaca tcttccaagg cgccgtccaa acccttctcg cgccagtcct
      481 tcttaagctc cccccacaca ttcgacaggc cgccgccgac acccccgccc ttttcccaag
      541 cctcgcccag ctccctgcgc gcttgggatt tgcgcccctc ctgaatcttc cgataatcca
      601 tatcctcggc cttgagccgg cccagttcct ctttgtcgcc cgtaagggca ttccacccca
      661 tacgcgccga acgcatagaa tttttaaacc cgcgcgtcaa cgccgtatcg tcggcggccg
      721 cccgcgcatc cttttgcgta aagccgcggt actccttcaa acccccggca agccgcccgt
      781 gttccaacgg atcgtatctg accaaatcac tcattgctca accctcttct cacccttctc
      841 attctcataa atgattttac cgccggccgt tttctgaccg ttcggcttaa agcccaactg
      901 cgcataacgc ttctcgcgct ctcccgcaaa ctccacatcc aaaggcttgc ccgtccgcaa
      961 atcgacaatc ccctcgcgct tcgccgtcaa gcccgtatcc gggtcggcaa cctcataact
     1021 gatagtcttg aacacattcg ggtcaaagcc cttctcccct tgccgctcaa acttcccgtt
     1081 aagcgcatca atctgccgcg caatctcccc gcgctgcccg tccgacttcg caaaaccata
     1141 agcccgcgtc aaatcggcga tgcgcttcct atcctcaagc tccagcgcat ccgcctcacg
     1201 cttcatctca aacccctgcc gcttcatccc cagctcggcc tgctgcacat caaacgccct
     1261 gtccgcattc ctctgcgcca tctcgcggtt caacgcaccc tcgttcgcct taaccgccgc
     1321 atcaaaccgc ccctcctgca acaccgcctc acggttcgcc gcattctccg ccgcagccgc
     1381 cgccagcctt tccctgccca attccctgac cgccgcattt ttattatgtt ccgccacaat
     1441 cccgcgcgcc gcattaagct gcgccgccgt caactgaccg ttcaaggccc ccgcatgagg
     1501 cgtagtcgcc ctcctgtaaa gctcccgctc ctcaggcgac agcgcatccg gcccggcaga
     1561 caccaaagga cgctgcgcgt caaaatcacg caccggtttg ccgtcccgat acacattccc
     1621 gtcaaatccc aaaggcggcg gcgtccgtcc gcccgcatcc gtcgggcgcg tatcgcgccg
     1681 cgcgaaatcc gccccggccc gcgcatcggg ggaaagcgcc ttatcgccgc ccgaaaaccc
     1741 caaaccggca tccgcacccc gttcgaaagc acccgcccgg cgcaaccgcc gcgccccatc
     1801 ctcaatattg ttacggctca aatccatcac attccgataa cgcgccggcc cggcctcata
     1861 attcgcaacg gtacgccgca ccgcatcggc acggtgcgcc tgtgccgccg cccgctcccg
     1921 ctcctccgcc cccttccggg ccttctcttt tttctccttt tccttcatct cccaaaaatc
     1981 cccggcagac ggctcaaaca aacccatcgc aaaccctccg aacaaataaa gcccaaaata
     2041 agcaaaaacc caaacccaat caaacgccta aagttaagaa caaacaaaaa tcgcaaccac
     2101 aaaccaatta ataaacaaca agttacacaa aaaaacacac cgacgggcca tttccggggg
     2161 caaataaaaa ccgccttcgt gcaaggcggt tgcaaaaaag cctttcattt aacgaaaggc
     2221 tttttgccgc ccgcgtaccg cgttcagagg ttgtccgaca ccgtcggcgt ttccatgcct
     2281 gtttgggcgt gattcgcact ctttggcggc acggccgtca tcgggccggt atccgcgccg
     2341 taggcttcag ggatgccttc gggcggtatt tgcgtttcag gcggcatggc ggggacgggg
     2401 aagccgggat cgtcgccccg cgccgccctg atgccttctt cgataccggt cgcgttttgc
     2461 acgaaaaccg acttgcgccc cagtccggtc aaaatctcca tcgtactttt gcctgtttgg
     2521 aagttgcgcg tcctgccgtc gtgcggcagg aagtccgtcc cccaccggta gggcagcttt
     2581 tccaattcgg caacatacca gtccaacgtc cggtgcgtgt cttcgatgta gccgatgatg
     2641 cgcacgctcg tcaaatcgcg ctgcaccagc ccgatggtca tggcatcgtt ccagcccaaa
     2701 tcccaaaccg tatgcacggg caaagaagaa tcataaggga cgagtgtaac gcgtccggaa
     2761 tgaaaagcgt cctgtatttc atgccggtaa accgccccct ccgataccat gcgcggcctg
     2821 ccttcccaaa tattcccgta gtcctcttta ttcatcgaac gctgtgcttt gagccgctcc
     2881 cggttcaatg cttcgggaaa ccacggattg tcgcgccagt tgatttcgca caaccaagtg
     2941 tcttcggacg gcatagcgat aaaacgccgg taggtttcgt ccgtctccat atcgggattg
     3001 agggtaatcc aaatttccga accttctttg cgtatggtcg gcgtgagcac gtcccagctt
     3061 tttttactga cgccgtgccc ttcctcaacc catacgatgt cgataccttc aaacgatttg
     3121 atgctgtcca cggtatgcga ctgcaggccc gaaaacacga acagcgtgcc gtttgcgccg
     3181 cgtatctcga agtcggttat ttcgtagaaa tgccccaaac ccaactgcgc cactttgtct
     3241 ttcaacaggc ggtgtacggt atcgcgcatc gatttttgaa tttcgcgcac gcataaaata
     3301 cgcaaagggc gttgcgcccc cagcgccagc agtgcggatg ccgcgccgtg cgatttgccg
     3361 ccgccgcgcc cgccgtacat aaccttgtac cggcaaggct tgaacagccc gtccagtttt
     3421 gcgggcaatt tcaaatcaac cgtctttccc gtcatcttca ggccctacaa attcgatacg
     3481 gatgcccgcc gtcttggcgg attccgaaat cttcgattcc gcggtatcca taccgtatga
     3541 tttacgctcg atttccacca aaaccttaaa ccccgccgcc accgtttgga acatacgcgc
     3601 ctttgtgaat gtgtcctccg ggcttccgta caattccgcg acaatctgct ccgcgctttt
     3661 ggacaggttt tccgccaaat tccgatgcct gcgctgtacg gcggcaaccc gtttcgcttc
     3721 cgcgtccgtt acttgtttaa ggttgaaatc cgcataatcc ggcgcattgc cgtttttgtc
     3781 cgcggacagc accatcgccc gcgcctttgc ctgtacctcg tcattcaaat cgcgcgtcca
     3841 cacgcgttct ttggcaaccc tgccgacgtg ttgcgccgaa acattgtatt tgcggccgat
     3901 ttcggcaatg ctcaaaaccc cgcgccggta atccagctcg actttgcgcc aatcttcatc
     3961 tgacaatctc ggcattaaag gtccaacctc ccgtttttgg cggaacattc cagataattc
     4021 cgcaatactg aaaactcgcg ccccatctga atactggaaa actgctgccc gaaccccgac
     4081 atcaaagccc ccaacgagcg catttcctgc gggttgaggt gcaataccgt atcgcctatt
     4141 tccaatacgc ccatccccga atccaatacc gatacgctga cggggcgcgt ctcgccaaac
     4201 cctatggcgg gcttgaaaat cccccttgtc acgcggtaga ttttgccgtg ctcgaccagc
     4261 agcttggtaa atttagtgat ttccgacatt ttcaggcctg tcgtttccgc caaagcctcg
     4321 cggctgacca cctgctccgc gttgtgcaaa tccacaaccc cctggtacac cttgtcaata
     4381 tccgacaatc ccgacatcaa atcaaactcc taaacatccc aaacgattcc aaactccgcc
     4441 gccgcccgcg attgcaggcg gttacactac aaagccaaaa tctttagatg ctgcctctct
     4501 cgctttcact gcatcttcaa tatgttcaaa taaacctaat attttatttt ttctattaac
     4561 cataatccta gcttgccatt tcttagacgg cttatggaaa taaaccccag tatgcccgct
     4621 agtattattt ttaaacttcc ctctattttt catattttca gcatgtgtaa catctctcaa
     4681 attagatatt ctattatctg tcttatccct attgatatgg tctattgaag aagcgtgttt
     4741 cccataaaca aaaagccaaa ttaatctatg tgcattatat tgtttcttaa atatttttaa
     4801 cttcaaatac cccaacctgc ctttgtataa gaaaacctcc ttgccggaaa atccggtatt
     4861 ccaagatttg taatgcaaac cgctattgaa atactttctt ggacgctccg cccaatataa
     4921 ctttcccgta ttgtcatcat atcttaataa ttcttttaaa acctcttgtg ttaatcccat
     4981 aaaacaccat gctccataga tgcccaagat ataatctttt cttgatattc agacatttcc
     5041 cgaactgata attttgtcgt tgatataccc attaattgcc cgttaggcat ttcaatacat
     5101 ccaataaatt ttctttttaa aaattcatgc catacatctt gactaaacct tctcccatcg
     5161 ataaaaactt tttctgaaat ttcacgatac aaaaaccata gccgcctgtt ctgctcgacg
     5221 ctccgttttg acttgcgcgg gcggattgtg atttcaaggt tgccgttttc aaaccacccg
     5281 tccgggctgt cccaaatcga ccgcataacc tgctttttgt tttggggcgt cagtgtaaat
     5341 tccgccccgt tcatttcaga cggcctttca cgccgacaac ccccaatgct tcaaggcggc
     5401 gtatcgtgcg caattgcgaa cggcgcatat aaaactcttt gtcttcgcgg cttaggcctg
     5461 cgcccctgcc gtcgatcgcg ccgcggcggc aaccgcaccc gaaacccgcg cccaaaccgc
     5521 cgcttttcaa tcccgccccg tgcgtctcgc ccgggaaacg gcacaacacg accgtttcag
     5581 ggttgtcatt gcacacgccc gcgatgttcg gcgtgcattg cccgcctttg gcggctttgc
     5641 gtatcgcgct agacatccca accctcctgt ccgatgaaat cccaaccctc gtctgccgca
     5701 tccgtctgtt ccat
//
LOCUS       DAWTQS010000089         5710 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.089, whole genome shotgun sequence.
ACCESSION   DAWTQS010000089 DAWTQS010000000
VERSION     DAWTQS010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5710
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.089"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(363..518)
                     /locus_tag="WNA25_001935"
     CDS             complement(363..518)
                     /locus_tag="WNA25_001935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824611.1"
                     /translation="MPSESPSDGIGFPKHSAPLSSCRKQTPIRFAANAQKTQCRLKRQ
                     TGFRRHR"
     gene            complement(535..2331)
                     /locus_tag="WNA25_001936"
     CDS             complement(535..2331)
                     /locus_tag="WNA25_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFA9824612.1"
                     /translation="MNTVSNYLSALREAMKAQGLDALVIPSADPHLSEYLPEHWQARR
                     ELSGFTGSVGTFVVTADEAGVWVDSRYWEQAAKQLAGSGIELQKSGQVPPYNEWLAAN
                     LPENAAVGIPSDMVSLTGKRTLAQSLAAKNIRIQHPDDLLDQVWTSRPAIPAETVFIH
                     DHAYVSETAAEKLARVRAVMAEKGADYHLVSSLDDIAWLTNLRGSDVPFNPVFVSFLL
                     IGKDSAVLFTEQCRLNAEAAAALQTAGITVEPYAQVADKLAQIGGALLIEPNKTAVST
                     LVRLPESARLIEGINPSTFFKSVKSEADIARIREAMEHDGAALCGFFAEFEDIIGKGG
                     SLTEIDVDTMLYRHRSARPGFVSLSFDTIAGFNANGALPHYSATPESHSTISGNGLLL
                     IDSGAQYKGGTTDITRVVPVGTPTAEQKRDNTLVLKAHIALAEAVFPENIPSPLIDAI
                     CRKPLWQAQCDYGHGTGHGVGYFLNVHEGPQRIAFAAPATPETAMKKGMVTSIEPGLY
                     RPGKWGIRIENLAANQAVADPQETEFGSFLCFETLTLCPIDTRLMDTALMTDGEIEWV
                     NRYHAEVRRRLEPLTEGAAKAWLIKRTEPLAR"
     gene            2789..4042
                     /locus_tag="WNA25_001937"
     CDS             2789..4042
                     /locus_tag="WNA25_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HFA9824613.1"
                     /translation="MSEQHISTWKSKINALGPGIMMASAAVGGSHLIASTQAGALYGW
                     QIALIIILTNLFKYPFFRFSAHYTLDTGKSLIEGYAEKSRVYLWVFLILCILSATINA
                     GAVAIVTAAIVKMAIPSLMFDAGTVAALIMASCLIILVSGRYRALDRVSKIIIVTLSI
                     ATLAAAGIAMSRGMQMQPDFIEPTPWTLAGLGFLIALMGWMPAPIEISAINSLWVTEK
                     QRINPSEYRDGIFDFNVGYIASAVLALVFLALGAFVQYGNGEAVQMAGGKYIGQLINM
                     YAVTIGGWSRPLVAFIAFACMYGTTITVVDGYARAIAEPVRLLRGKDKTGNAEFFAWN
                     IWVAGSGLAVIFWFDGVMANLLKFAMIAAFVSAPVFAWLNYRLVKGDEKHKLTSGMNA
                     LALAGLIYLTGFTVLFLLNLAGMFK"
     gene            4197..5708
                     /gene="lysS"
                     /locus_tag="WNA25_001938"
     CDS             4197..5708
                     /gene="lysS"
                     /locus_tag="WNA25_001938"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFA9824614.1"
                     /translation="MSEQNHPQTEPQLDENQIIALRREKLHNIRQQRNAYPNDFKRDS
                     FAADLQAQYGEIGKEELDPQAVPVKIAGRMMLKRQMGKASFATIQDVTGQIQLYLNNK
                     GVSQEVLDDFNHWDLGDIVGAEGTLFKTNHGELTVRVSDIRLLSKSLRPLPDKHKGLS
                     DQETKYRQRYVDLIANEESRNTFIKRSQIIQSVRNFMVGEHYLEVETPMMHPIPGGAT
                     AKPFVTHHNALDIPLYLRIAPELYLKRLVVGGLERVFEINRSFRNEGMSVRHNPEFTM
                     IEFYEAFSDYERMMQMAEDIIRNASRTVNGTANITYNGKEVDLESPFERLTILEAIKK
                     YNPHYTDEQLNDAEWLKKEIVKHGESLPPSPGIGSLQLALFEGCAEGKLWNPTFIVDY
                     PVEVSPLARASDTKQGLTERFELFVVGRELANGYSELNDPEDQAERFKSQVAQKDAGD
                     DEAMHYDADYIRAMEFGLPPTGGCGIGIDRLVMLLTDLQTIRDVILFPQMRPE"
BASE COUNT         1201 a         1489 c         1678 g         1342 t
ORIGIN      
        1 cctccccggc cctcccccac gggggaggga gcagattgcg gcagatttgg cggttgcagg
       61 cggtttgatt tgcgagggaa tcggacgcgc aggcgggaat ccggatttgt ccgcgcggaa
      121 acttatattt cgtcattcca ccaatcccgc ccacagccca cttttccgcg tcattcccgc
      181 gcaggcggga atccagtccg ttcggtttcg gttatttccg ataaattcct gctgcttttc
      241 attcctagat tcccactttc gcgggaatga cgggatttca gggtaaagat ggcgggagcc
      301 ggagggctgt gaagagctgt tttcgcagga atgacgatat gcggatttgc tgttttcatc
      361 cgctaccgat gccgtctgaa gcctgtttgg cgtttcagac ggcattgggt tttttgtgca
      421 tttgccgcaa aacggatcgg tgtctgcttg cggcaagacg ataacggtgc ggaatgtttt
      481 gggaaaccaa tgccgtctga agggctttca gacggcattt ttgtaccgtg ccgtttaacg
      541 cgccagcggt tcggtgcgtt tgatcagcca cgcttttgcc gcgccttcgg tcagcggctc
      601 gaggcggcgg cggacttcgg cgtggtagcg gttgacccat tcgatttcgc cgtcggtcat
      661 cagggcggtg tccatcaggc gtgtatcgat ggggcagagg gtcagggttt caaaacagag
      721 gaagctgccg aactcggttt cctgcgggtc ggcgacggct tggttggcgg cgaggttttc
      781 aatgcggatg ccccattttc ccggacggta gaggccgggt tcgatggagg taaccatgcc
      841 ttttttcatg gcggtttcgg gcgtggcggg ggcggcgaag gcgatgcgct gcgggccttc
      901 gtggacgttg aggaaatagc ctacgccgtg gccggtgccg tgtccgtagt cgcattgcgc
      961 ctgccacagg ggtttgcggc aaatcgcgtc aatcagcggc gaggggatgt tttcggggaa
     1021 cacggcttcg gcaagcgcga tatgggcttt gagaacgagc gtgttgtcgc gtttttgttc
     1081 tgctgtgggc gtgccgacgg ggacgacgcg ggtgatgtcg gtcgtgccgc ctttgtattg
     1141 cgcgccggag tcgatgagca gcagaccgtt gccgctgatg gtgctgtggc tttcgggtgt
     1201 tgcgctgtaa tgcggcaggg cgccgttggc gttgaagcct gcgatggtgt cgaaactcaa
     1261 ggacacaaaa cccgggcgcg cgctgcggtg gcggtaaagc atcgtatcca cgtcgatttc
     1321 ggtcaggctg ccgcctttgc cgatgatgtc ttcaaactcg gcgaagaaac cgcacaatgc
     1381 cgcgccgtcg tgttccatcg cttcgcggat gcgggcgatg tcggcttcgg atttgacgga
     1441 tttgaagaag gtggacgggt tgatgccttc aatcaggcgc gcgctttcgg gcaggcgcac
     1501 gagcgtactg acggcggttt tgttcggctc gatgagcagc gcgccgccga tttgcgcgag
     1561 tttgtcggca acttgggcgt aaggctcgac cgtgatacct gcggtttgca gcgcggcggc
     1621 ggcttcggcg ttcagacggc attgttcggt aaacaggacg gcactgtctt tgccgatcag
     1681 caggaaggac acgaaaacag ggttgaaagg cacgtcgctg ccgcgcaggt tggtcagcca
     1741 agcgatgtcg tcaagcgagg aaaccaagtg gtaatccgcg cctttttccg ccataactgc
     1801 gcgcacgcgg gcgagtttct cggcggcggt ttcggagacg taggcgtggt cgtggatgaa
     1861 cacggtttcg gcggggatag cgggacggct tgtccacact tggtcaagca ggtcgtcggg
     1921 gtgttggatg cggatgtttt tggcggcgag cgattgcgcc aaagtgcgtt tgccggtgag
     1981 cgacaccata tcggaaggga tgccgacggc agcgttttcg ggcaggtttg ccgcgagcca
     2041 ttcgttgtac ggcggcactt gcccgctttt ttgcagttcg atgccgctgc ccgcaagctg
     2101 cttggcggct tgttcccaat agcggctgtc cacccatacg cccgcttcgt cggcggtaac
     2161 gacgaatgtg ccgaccgagc cggtaaagcc cgacaattcg cgccgcgcct gccaatgttc
     2221 gggcaggtat tcggacaggt gggggtcggc ggaagggatg acgagtgcat ccaagccttg
     2281 cgccttcatg gcttcgcgca atgcggacag gtaattcgat acggtattca taataattcc
     2341 tccaatcggg ttttgcggct tcagacggca tggacggaaa tctgaaatgc cgtctgaaaa
     2401 acataaccat cttatcaaat cgccagcctg ctgcaaaacg gacgggctga tcggatggtg
     2461 cgtgtttgcg cgaaaaaagc gatgtaaggt tgtttgttgt aaaattttag gcataaaatg
     2521 tcgtttcatg cctttttttg gaagtaatac ggaaaatata gtcaagtttg agcatgaagt
     2581 tctaatagtg tgtgttggca ggtatttgga ttacgttaca atacgcgctt tcatcaaaat
     2641 ttccctaccg ggcggtatcc tgtttttgaa gacggtagga tttgggtttg tttgggggca
     2701 atcccgccca aattggagca atagataggg ctgtgtccga atatgcggct ctgtgtgttg
     2761 aaacacggtt aaaaatacgg aagactttat gtccgaacaa catatttcga cttggaaaag
     2821 taaaatcaac gcattgggac cggggattat gatggcttcg gcggcggtcg gcggttcgca
     2881 cctgattgcc tcgacgcagg cgggcgcgct ttacggctgg cagatcgcgc tcatcatcat
     2941 cctgaccaac ctcttcaaat acccgttttt ccgcttcagc gcgcattaca cgctggacac
     3001 gggcaagagc ctgattgaag gttatgccga gaaaagccgc gtttatttgt gggtattcct
     3061 gattttgtgc atcctctccg ccacgattaa cgcgggcgcg gtcgccattg tcaccgccgc
     3121 catcgtcaaa atggcgattc cctcgctgat gtttgatgcc ggcacggttg ccgccttgat
     3181 tatggcatcc tgcctgatta ttttggtgag cggacgttac cgcgctttgg atcgcgtttc
     3241 caaaatcatc atcgttactt tgagtatcgc cacgcttgcc gccgccggca tcgctatgtc
     3301 gcgcggtatg cagatgcagc cggactttat cgagccgaca ccgtggacgc ttgccggttt
     3361 gggcttcctg atcgcgctga tgggctggat gcctgcgccg attgaaattt ccgccatcaa
     3421 ttctttgtgg gtaaccgaaa aacaacgcat caatccttcc gaataccgcg acgggatttt
     3481 tgatttcaac gtcggttata tcgccagtgc ggttttggct ttggttttcc ttgcactggg
     3541 cgcgtttgtg caatacggca acggcgaagc agtgcagatg gcgggcggca aatatatcgg
     3601 gcaattgatc aatatgtacg ccgttaccat cggcggctgg tcgcgcccgc tggtggcgtt
     3661 tatcgcgttt gcctgcatgt acggcacgac gattaccgtt gtggacggct atgcccgtgc
     3721 cattgccgaa cccgtgcgcc tgctgcgcgg aaaagacaaa acgggcaacg ccgaattctt
     3781 tgcctggaat atttgggtgg cgggcagcgg tttggcggtg attttctggt ttgacggcgt
     3841 aatggcgaat ctgctcaaat ttgcgatgat tgccgctttt gtgtccgccc ccgtgtttgc
     3901 ctggctgaat taccgtttgg tcaaaggtga tgaaaaacac aaactcacat caggtatgaa
     3961 tgcccttgca ttggcaggct tgatttatct gaccggtttt accgttttgt tcctattgaa
     4021 tttggcggga atgttcaaat gataacgatg ccgtctgaaa ccgcaaaccg ctttcagacg
     4081 gcattgccgc gtttatggaa aaaaatgccg acatcgcgta aaatatgcgc aaattttgtg
     4141 attcggtcag gtcgtctgaa acagattgcg cctgatttat tttttcggaa aaccctatga
     4201 gcgaacaaaa ccatccgcaa accgagccgc agttggacga aaaccaaatc atcgccctgc
     4261 gccgcgaaaa actgcacaac atccgccaac agcgcaacgc ttaccccaac gatttcaagc
     4321 gcgacagctt tgccgccgat ttgcaggcgc aatacggcga aatcggcaaa gaagaactcg
     4381 acccgcaggc cgttcccgtt aaaattgccg gccgcatgat gctcaaacgt caaatgggca
     4441 aagccagctt cgccaccatt caagacgtta ccggtcaaat ccagctttat ctgaacaaca
     4501 aaggcgtgag ccaagaagtt ttggacgact tcaaccactg ggacttgggc gacatcgtcg
     4561 gtgcggaagg cactttgttc aaaaccaacc acggcgaact aaccgtgcgt gtgtccgaca
     4621 tccgcctgct gtccaaatcc ctgcgtccgc tgcccgacaa acacaaaggc ttgagcgatc
     4681 aggaaaccaa ataccgccaa cgctacgtcg atttgattgc caatgaagaa tcgcgcaata
     4741 cctttatcaa acgcagccaa atcatccaat ctgtgcgtaa ttttatggtg ggcgagcatt
     4801 atctcgaagt cgaaaccccg atgatgcacc cgattcccgg cggcgcgaca gcgaaaccct
     4861 tcgttaccca ccacaatgcc ttggacattc cgctctacct gcgcatcgcg cccgagctgt
     4921 atctgaaacg cttggtggtc ggcggcttgg aacgcgtgtt tgaaatcaac cgcagcttcc
     4981 gtaacgaggg catgtccgtg cgccacaacc ccgaatttac gatgattgaa ttttacgaag
     5041 ccttctccga ctacgaacgt atgatgcaga tggcggaaga catcatccgc aacgcatcgc
     5101 gcacggtaaa cggcacggca aacatcactt acaacggcaa agaagtcgat ttggaaagcc
     5161 cgttcgaacg tctgaccatc ctcgaagcca tcaaaaaata caatccgcac tacaccgacg
     5221 agcagttgaa cgatgcggaa tggctgaaaa aagaaatcgt caaacacggc gaaagcctgc
     5281 cgccgtcccc gggcatcggc agcctgcaac tcgccctgtt tgaaggttgc gccgagggca
     5341 aactgtggaa tccgaccttt attgtcgatt acccggtcga agtttccccg ttggcgcgcg
     5401 cttcggatac gaaacaaggt ctgaccgaac gcttcgagct tttcgttgtc gggcgcgaac
     5461 tggcaaacgg ctattccgag ttgaacgacc ccgaagacca agccgaacgc ttcaaatcgc
     5521 aagtggcgca aaaagatgcg ggcgacgacg aagccatgca ctacgatgcc gactacatcc
     5581 gcgcgatgga attcggtttg ccgccgaccg gcggttgcgg catcggcatc gaccgcttag
     5641 tgatgctgct gaccgatttg caaaccatcc gcgatgtgat tctgtttccg cagatgcgtc
     5701 ccgagtaatc
//
LOCUS       DAWTQS010000090         5413 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.090, whole genome shotgun sequence.
ACCESSION   DAWTQS010000090 DAWTQS010000000
VERSION     DAWTQS010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5413
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.090"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(<1..3444)
                     /gene="app"
                     /locus_tag="WNA25_001939"
     CDS             complement(<1..3444)
                     /gene="app"
                     /locus_tag="WNA25_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_168458961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesion and penetration autotransporter App"
                     /protein_id="HFA9824615.1"
                     /translation="MKTTDKRTTETHRKAPKTGRIRFSPAYLAICLSFGILPQARAGH
                     TYFGINYQYYRDFAENKGKFAVGAKDIEVYNKKGELVGKSMTKAPMIDFSVVSRNGVA
                     ALAGDQYIVSVAHNGGYNNVDFGAEGSNPDQHRFSYQIVKRNNYKAGTNGHPYGGDYH
                     MPRLHKFVTDAEPVEMTSYMDGWKYADLNKYPDRVRIGAGRQYWRSDEDEPNNRESSY
                     HIASAYSWLVGGNTFAQNGSGGGTVNLGSEKIKHSPYGFLPTGGSFGDSGSPMFIYDA
                     QKQKWLINGVLQTGNPYIGKSNGFQLVRKDWFYDEIFAGDTHSVFYEPHQNGKYFFND
                     NNNGAGKIDAKHKHYSLPYRLKTRTVQLFNVSLSETAREPVYHAAGGVNSYRPRLNNG
                     ENISFIDKGKGKLILTSNINQGAGGLYFEGNFTVSPKNNETWQGAGVHISDGSTVTWK
                     VNGVANDRLSKIGKGTLLVQAKGENQGSVSVGDGKVILDQQADDQGKKQAFSEIGLVS
                     GRGTVQLNADNQFNPDKLYFGFRGGRLDLNGHSLSFHRIQNTDEGAMIVNHNQDKEST
                     VTITGNKDITTTGNNNNLDSKKEIAYNGWFGEKDATKTNGRLNLNYQPEEADRTLLLS
                     GGTNLNGNITQTNGKLFFSGRPTPHAYNHLGSGWSKMEGIPQGEIVWDNDWIDRTFKA
                     ENFHIQGGQAVVSRNVAKVEGDWHLSNHAQAVFGVAPHQSHTICTRSDWTGLTSCTEK
                     TITDDKVIASLSKTDIRGNVSLADHAHLNLTGLATLNGNLSAGGDTHYTVTRNATQNG
                     NLSLVGNAQATFNQATLNGNTSASDNASFNLSNNAVQNGSLTLSDNAKANVSHSALNG
                     NVSLADKAVFHFENSRFTGKISGGKDTALHLKDSEWTLPSGTELGNLNLDNATITLNS
                     AYRHDAAGAQTGSAADAPRRRSRRSLLSVTPPTSAESRFNTLTVNGKLNGQGTFRFMS
                     ELFGYRSGKLKLAESSEGTYTLAVNNTGNEPVSLEQLTVVEGKDNTPLSENLNFTLQN
                     EHVDAGAWRYQLIRKDGEFRLHNPVKEQELSDKLGKAGETEAALTAKQAQLAAKQQAE
                     KDNAQSLDALIAAGRDATEKAESVAEPARQAGGENAGIMQAEEEKKRVQADKDTALAK
                     QR"
     gene            3882..5228
                     /gene="mnmE"
                     /locus_tag="WNA25_001940"
     CDS             3882..5228
                     /gene="mnmE"
                     /locus_tag="WNA25_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFA9824616.1"
                     /translation="MSDNVPTIAAVATAPGRGGVGVIRISGKNLLPMAQALCGKTPEP
                     RVATYADFTDADGQAIDSGLLLFFAAPASFTGEDVIELQGHGGPVVMEMLLNRCLELG
                     ARLAEPGEFTKRAFLNDKLDLAQAEGVADLIDASGRSAARLALRSLKGDFSRRIHGLV
                     EGLITLRMLVEAALDFPEEDIDFLEAADARGKLDGLRRAVDDVLANAQQGAILREGLN
                     VVLVGAPNVGKSSLLNALAGDEVAIVTDIAGTTRDAVRERILIDGVPVHIVDTAGLRE
                     TDDVVERIGIERSRKAVSEADVALVLVDPREGLNEKTRMILDTLPSDLKRIEIHSKSD
                     LHAHAAGGFGTGAETVIALSAKTGDGLDALKRTLLCEAGWQGESEGLFLARTRHVNAL
                     KAAQEELSLAALCGNHQIELFAEHLRLAQVACGEITGEFTADDLLGVIFSRFCIGK"
BASE COUNT         1094 a         1317 c         1499 g         1503 t
ORIGIN      
        1 gcgctgtttc gccaaggcgg tgtctttatc cgcctgcacc cgttttttct cttcctccgc
       61 ctgcataatg ccggcatttt ccccgcctgc ctgccgggcc ggttcggcaa cgctttctgc
      121 cttttcggtg gcatcgcgcc cggccgcaat cagcgcgtca aggctttgcg cgttgtcttt
      181 ttccgcctgt tgtttggcgg caagttgtgc ctgttttgcc gtcaaggcgg cctctgtttc
      241 tcccgccttg ccgagtttgt cggaaagctc ttgttctttg accggattat gcaggcggaa
      301 ctcgccgtct ttgcggataa gctgataacg ccatgcgccg gcatcgacgt gttcgttttg
      361 cagggtgaaa ttaagatttt cggacagcgg tgtgttgtct tttccttcca ctaccgtcaa
      421 ttgctcgaga cttacgggtt cgttgccggt attgttgaca gccaaggtgt aagtgccttc
      481 ggaactttcc gccagcttca atttgccgct gcggtagccg aagagttccg acataaagcg
      541 gaatgttccc tgaccgttca atttgccgtt taccgtcagc gtgttgaaac gggattctgc
      601 cgaagttggc ggcgtaacgg ataataggga acggcgcgaa cggcggcgcg gcgcatctgc
      661 cgcactgccg gtttgcgcgc ctgccgcatc gtgtcgatag gcggaattga gtgtaatggt
      721 ggcgttgtca aggtttaaat tgcctaattc cgtgcccgac ggcagcgtcc attcgctgtc
      781 ttttaagtgt aatgccgtat ccttgccgcc gctgattttt ccggtaaagc ggctgttttc
      841 aaaatggaat actgccttat cggctaggga gacattgccg ttgagtgcgg aatggcttac
      901 gtttgcctta gcgttgtcgg aaagcgtcag actgccgttt tgtacggcgt tgttgcttag
      961 attaaatgaa gcattgtccg aagccgatgt gttgccgttt aatgtggctt gattaaatgt
     1021 tgcttgggca ttgcccacga ggctaaggtt gccgttttgg gtggcgttgc gcgtaaccgt
     1081 atagtgcgtg tctccgcctg cactaagatt gccgttgagt gtggcaagtc ctgtgagatt
     1141 taaatgagcg tgatcggcaa ggctgacatt gcctctgatg tcggtcttgc tcaatgaagc
     1201 aatcacttta tcgtcggtaa tggttttttc ggtacaactt gtcagacccg tccagtccga
     1261 acgtgtacag attgtgtggc tttgatgcgg tgcgacaccg aaaactgctt gggcgtgatt
     1321 gcttaaatgc caatcgcctt ccactttggc aacattgcgg gaaaccaccg cttgtccgcc
     1381 ctgaatatgg aagttttccg ctttaaatgt gcggtcgatc caatcgttgt cccacacgat
     1441 ttctccttgt gggatacctt ccatttttga ccacccgctt cctaaatgat tgtaggcgtg
     1501 cggtgtcggt ctgccgctga aaaacagttt gccgtttgtt tgcgtgatat tgccgtttaa
     1561 atttgttccg ccggaaagca gtaaagtgcg atccgcttct tccggttggt aattcagatt
     1621 gagccgcccg ttcgttttgg ttgcatcttt ctcgccaaac caaccgttgt aggcaatttc
     1681 ttttttgcta tccaagttgt tgttattgcc ggttgtagta atatctttat tgcctgtaat
     1741 ggtaacggtg gattctttgt cttgattgtg gttgacaatc atcgcccctt catcggtatt
     1801 ttgaatgcgg tggaacgaaa gcgaatgccc gttcaaatcc aaacgtccgc cgcgaaagcc
     1861 gaaatagagt ttgtcggggt tgaactgatt atcggcattc agttgcaccg tccccctgcc
     1921 gctgaccaag ccgatttcac taaaggcttg ttttttgcct tgatcgtccg cctgctgatc
     1981 taagatgact ttaccgtcgc ccacgctgac cgagccttgg ttttcccctt tggcttgaac
     2041 cagcagcgtg cctttgccga ttttggacag gcggtcgttt gccacgccgt ttactttcca
     2101 agtaacggta ctgccatcac tgatatgaac gcccgcgcct tgccacgttt cgttgttttt
     2161 aggcgagacc gtaaaattac cctcaaaata caaaccgccc gcgccttggt tgatgttgct
     2221 ggtaagtatc aatttacctt ttcctttgtc aataaaggaa atattttctc cattattcag
     2281 tctgggtcga taactgttga ccccacctgc agcatgataa acaggttctc ttgctgtctc
     2341 ggataaagaa acattaaaca attgaacggt tcgtgttttt aatctataag gtagagaata
     2401 gtgtttatgt ttggcatcga tttttcctgc gccattatta ttgtcgttaa aaaagtattt
     2461 cccattttga tgtggttcgt agaatactga atgggtatct ccagcaaaga tttcatcata
     2521 gaaccaatct ttacgaacta gctggaagcc attgcttttt cctatatagg ggttgcctgt
     2581 ttgcaatacc ccattaatta accacttttg cttttgggca tcatagataa acattggtga
     2641 gccactgtcg ccaaatgagc ctcctgttgg taaaaaacca tatgggctat gtttaatttt
     2701 ttcgctacct aagttgactg tgccaccacc tgatccattt tgtgcaaagg tattgccacc
     2761 gacgagccaa gaatatgcgc ttgcaatatg atatgaactt tcgcggttat tgggttcgtc
     2821 ttcatcagac cgccaatatt gtctgcctgc tccgattcga acacgatcag ggtatttatt
     2881 taaatcagcg tatttccacc catccatata actggtcatc tcaacaggtt ctgcatctgt
     2941 gacaaatttg tgcaaacgcg gcatatgata atcgccgcca taaggatggc cgttagtccc
     3001 tgctttataa ttatttcttt tcacaatttg gtaagaaaag cggtgctgat cgggattgct
     3061 tccctccgca ccaaaatcaa cattgttata gccgccgtta tgtgccacgc tcacaatata
     3121 ttgatcgccc gccaatgccg ccacgccgtt acgcgatacc acagaaaaat caatcatcgg
     3181 ggctttcgtc atcgatttgc cgaccaactc cccttttttg ttgtaaacct caatatcttt
     3241 cgccccgact gcaaacttgc ctttattttc ggcaaagtcg cgatagtatt ggtagttgat
     3301 gccgaaataa gtgtgtcccg cccgggcttg gggcagaatg ccgaacgaca ggcatatggc
     3361 taagtaagcg ggcgagaagc ggatgcggcc ggttttaggg gctttgcggt gtgtttcggt
     3421 tgtccgtttg tcggttgttt tcattatttt tccttatctg acgggattcg gtttgtttgg
     3481 gagggcgcgg cttccgcttc cgggcggcgc gcgggatgtg gctatatgtg cggttcggcg
     3541 tattttgccg cttctgttcg gagatggtgc agggaagggc gggaaggaaa aaaaggggat
     3601 ttttatcaac tcgccggagc aatcgatggg ttgcacataa aggcatcatt ttgaatacga
     3661 aatcatatca cactttactt tatccccgtt ccttttcggt cggaaccccg ccgttttcgg
     3721 cggtaaaaac cgaccggagg cggaatgcgg ggcagggttt tgtgccgtgc ggtgtcggaa
     3781 tatcgtttta cacaagccgg caatgccatc tgaaagccga accgtcggac ttcagacggc
     3841 atttgctata atcgcggctg ttttgaattt tcgggggttt tatgtcggat aacgttccaa
     3901 cgattgcggc agtcgcgacc gcgcccggac gcggcggcgt gggcgtgata cgcatatcgg
     3961 ggaaaaacct gttgccgatg gcgcaggctt tgtgtgggaa aacgcccgag ccgcgcgtgg
     4021 caacctatgc tgattttacg gacgcggacg gacaggcaat cgatagcggg ctgctactgt
     4081 tttttgccgc gccggcaagt tttacgggcg aagatgtcat cgagcttcag ggacacggcg
     4141 ggccggtagt gatggagatg ctgctgaacc gctgtttgga actgggcgcg cgccttgccg
     4201 agccgggcga gtttaccaaa cgtgcgtttt taaacgacaa actggacttg gcacaggcgg
     4261 aaggcgtggc ggatttgatt gacgcatccg gccgttcggc ggcgcgtctg gcgttgcgct
     4321 cgctcaaggg cgatttttcg cggcggatac acggtttggt cgaaggcttg attaccttgc
     4381 ggatgctggt cgaagcggcg ctggattttc ccgaggaaga catcgatttt ctcgaagcgg
     4441 cggacgcacg cggcaaactg gacggcttgc gccgcgccgt ggatgatgtg cttgccaacg
     4501 cgcagcaggg cgcgatttta cgcgaaggtc tgaatgtcgt attggtcggt gcgccgaatg
     4561 tcggcaagtc cagcctgttg aacgcgctgg cgggcgacga agtggcgatt gttaccgata
     4621 ttgccggaac gacgcgcgac gcggtcaggg aacgtatcct gattgacggc gtgccggtgc
     4681 atattgtcga tacggcaggt ttgcgcgaga cggacgacgt ggtcgaacgc atcggcatcg
     4741 aacgcagccg caaagccgta tccgaagccg atgtcgcgct ggtgttggtc gatccgcgcg
     4801 agggtttgaa tgaaaagacg cggatgattt tggatacgtt gccgtcggat ttgaaacgca
     4861 ttgaaatcca cagcaaatcc gatttgcacg cacacgcggc aggcgggttc ggtacgggcg
     4921 cggaaaccgt catcgcgctg tcggcgaaaa ccggcgacgg cttggacgcg ctgaaacgga
     4981 cgctgttgtg cgaggccggt tggcagggcg aaagcgaagg gctgtttttg gcgcggacgc
     5041 ggcacgtcaa cgcactcaag gcggcgcagg aagaattgtc gctggccgct ttgtgcggca
     5101 accatcaaat cgagctgttt gccgaacact tgcggctggc tcaggtcgcg tgcggcgaaa
     5161 ttacggggga atttacggcg gacgacctgc tcggcgtgat tttttcgagg ttttgcatcg
     5221 gaaaataagc gggccggagg cattgtggcg gtgtccggct gaacattccg ttatcccata
     5281 aaaacgggaa tccgatccgt tcggttttgg ttttttgaat ttcgggcaac gcttaaatct
     5341 tcattccgcg caggcggaaa ttatcggcgc ggtacggcaa cttttttcaa tactgaaagc
     5401 cccgtcattc ctg
//
LOCUS       DAWTQS010000091         5385 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.091, whole genome shotgun sequence.
ACCESSION   DAWTQS010000091 DAWTQS010000000
VERSION     DAWTQS010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5385
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.091"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(<1..848)
                     /gene="mafA"
                     /locus_tag="WNA25_001941"
     CDS             complement(<1..848)
                     /gene="mafA"
                     /locus_tag="WNA25_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HFA9824617.1"
                     /translation="MRARLLIPILFSVFILSACGTLTGIPSHGGGKRFAVEQELVAAS
                     ARAAVKDMDLQALHGRKVALYIATMGDQGSGSLTGGRYSIDALIRGEYINSPAVRTDY
                     TYPRYETTAETTSGGLTGLTTSLSTLNAPALSRTQSDGSGSRSSLGLNIGGMGDYRNE
                     TLTTNPRDTAFLSHLVQTVFFLRGIDVVSPANADTDVFINIDVFGTIRNRTEMHLYNA
                     ETLKAQTKLEYFAVDRTNKKLLIKPKTNAFEAAYKENYALWMGPYKVSKGIKPTEGLM
                     VDFSDIR"
     gene            complement(979..1698)
                     /locus_tag="WNA25_001942"
     CDS             complement(979..1698)
                     /locus_tag="WNA25_001942"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFA9824618.1"
                     /translation="MTQQIKYKRVLLKLSGESLMGSDPFGINHDTIVQTVGEIAEIVK
                     MGVQVGIVVGGGNIFRGVSAQAGSMDRATADYMGMMATVMNALALKDAFETLGIKARV
                     QSALSMQQIAETYARPKAIQYLEEGKVVIFAAGTGNPFFTTDTAAALRGAEMNCDVML
                     KATNVDGVYTADPKKDPSATRYETITFDEALNKNLKVMDATAFALCRERKLNIVVFGI
                     AKQGSLKRVITGEDEGTLVHC"
     gene            complement(1915..2769)
                     /locus_tag="WNA25_001943"
     CDS             complement(1915..2769)
                     /locus_tag="WNA25_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HFA9824619.1"
                     /translation="MAEITAKMVADLRAATGLGMMECKKALVEAEGNFDKAEEILRIK
                     SGAKAGKLAGRTAAEGVLAYAINGNVGALVEVNCETDFVAKDAGFVEFANFVAKTAAE
                     KKPASVEELSELVESERKAIIAKLGENMSVRRFQVIDTANQLVAYIHGALATEGVLVE
                     YKGSEDVARKIGMHIVAAKPQCVSEAEVDAETVEKERHIYTEQAIASGKPADIAAKMV
                     EGRIRKFLAEITLNGQAFVMNPDQTVAQFAKENDTEVVSFVRYKVGDGIEKAVVDYAA
                     EVAAAAKV"
     gene            complement(2898..3626)
                     /gene="rpsB"
                     /locus_tag="WNA25_001944"
     CDS             complement(2898..3626)
                     /gene="rpsB"
                     /locus_tag="WNA25_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFA9824620.1"
                     /translation="MSQITMRQMIEAGVHFGHQTRFWNPKMAQYIFGARNKIHIVNLE
                     KTLPMFQEAQEAVRRLVANKGTVLFVGTKRQARDIIREEATRAGMPFVDHRWLGGMLT
                     NYKTVKQSIKRLEEKTAALENAAESGFSKKEILEMQRDVEKLERSLGGIKNMKGLPDA
                     IFVIDTGYQKGTLVEAEKLGIPVIAVVDTNNSPDGVKYVIPGNDDSAKAIRLYCRGIA
                     DAVLEGKNQALQETVAAAQETAAE"
     gene            complement(3799..4032)
                     /locus_tag="WNA25_001945"
     CDS             complement(3799..4032)
                     /locus_tag="WNA25_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824621.1"
                     /translation="MWHIVAIGYLFVAVMYSAAQPSIARALIYLVFWVVLPTVFTVFA
                     VTVRRRNRLMGQQEQAESEQQRAQRQKDSGTKP"
     gene            4110..4691
                     /locus_tag="WNA25_001946"
     CDS             4110..4691
                     /locus_tag="WNA25_001946"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFA9824622.1"
                     /translation="MRNEEKRALRRELRGRRSQMGRDVRAAAAVKINRLLKRYIKRGR
                     KIGVYWPMGKELRLGGFVRAAQKRGAKLYLPYIEPHTRRMWFTPYPERGMERERKRGR
                     AKLHVPQFAGRKIRVHGLSVLLVPLVGIDREGYRLGQAGGYYDATLSAMKYRLQAKTV
                     GVGFACQLVDRLPREAHDLPLDGFVSEAGILCF"
     gene            4752..5342
                     /locus_tag="WNA25_001947"
     CDS             4752..5342
                     /locus_tag="WNA25_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFA9824623.1"
                     /translation="MSERIILPVLSLGGVRLEPLDVHHETGLREAVCDGEVWKLGVTS
                     APHPDRVADYIGTALATRLAFAVVDEEADRVVGTTAYYHFEPQIPRLDIGFTWYAASA
                     RRKRINTCCKIMLLDYAFDVLACCCAGWRTDILNLASQRAIERLGAEKDGVLRMHMLR
                     KDGSVRDTVVYSMLREDWCKNREILTGRLAGYGVQV"
BASE COUNT         1185 a         1253 c         1508 g         1439 t
ORIGIN      
        1 cggatatcgg agaaatcgac catcaatcct tccgtcggtt tgattccttt gcttacttta
       61 tacggcccca tccacaatgc gtaattttct ttataggcag cttcaaacgc attggttttg
      121 ggtttgatga gcaatttttt attggttctg tctactgcga aatattccag ttttgtttgg
      181 gctttcagtg tttcggcatt gtataggtgc atttcggttc tgttgcgtat cgttccgaat
      241 acgtcgatgt taataaacac atctgtatcg gcattggcag gagaaacaac gtctatgccg
      301 cgcaggaaaa atacggtctg taccaagtgg gaaagaaagg cagtgtcgcg cgggttggtc
      361 gtcaaggttt catttcgata atcccccatc ccgccaatat ttaagcccag actgctccta
      421 cttccgctac cgtctgattg ggtgcgcgag agtgcagggg cattaagtgt agataaagaa
      481 gtggttaaac ccgtcaaacc gcctgatgtt gtttcagcgg tggtttcgta acgcggatag
      541 gtgtaatcgg tacggacggc agggctgttt atgtattcgc cgcgaatcag tgcatcaatg
      601 gagtagcgac cccctgtcaa actgcctgaa ccttggtcgc ccatagttgc aatgtacaat
      661 gcaacttttc gtccgtgtaa tgcctgtaaa tccatgtctt taacggcagc tctggcagaa
      721 gcggccacaa gttcttgttc gaccgcgaag cgtttgcctc cgccatgcga tggaatacct
      781 gtcagtgtcc cgcaggcgga taaaataaaa actgaaaaaa gaataggtat cagcagccgt
      841 gcccgcatag attttctcct ttatgaaaaa caaattgtat caaaattgta aatttaatcc
      901 actataatct ccatataata aatggctgtc tgaagcccat atgcgactat atctgccgac
      961 cctatggtca atcatcaatc agcagtggac cagtgtgcct tcgtcttcgc cggtaatgac
     1021 gcgtttgagc gagccttgtt tggcgatgcc gaagacgacg atattgagct tgcgttcgcg
     1081 gcagagggca aaggcggtcg cgtccatgac tttgaggttt ttattcaagg cttcgtcaaa
     1141 agtaatggtt tcgtagcgtg tggcggacgg gtcttttttc gggtctgcgg tgtacacgcc
     1201 gtcgacgttg gtggctttga gcatcacgtc gcagttcatt tccgcgccgc gcaatgcggc
     1261 ggcagtgtcg gtcgtgaaga acggattacc ggtaccggcg gcgaaaatca cgactttgcc
     1321 ttcttccaaa tattgaatgg ctttaggacg ggcgtaagtt tcagcgattt gctgcataga
     1381 cagtgcggat tgtacgcgcg ctttgatgcc caaggtttca aatgcgtctt tgagtgccaa
     1441 ggcgttcatc acggtcgcca tcatgcccat gtagtcggca gtggcgcggt ccatgctgcc
     1501 tgcttgtgca gatacgccgc ggaaaatgtt accgccgccg acaacgatac cgacttgcac
     1561 acccattttc acgatttcgg caatttcgcc gacagtttga acaatggtat cgtgattgat
     1621 gccgaacgga tcggaaccca tcagggattc gccggagagt ttcagtaata cgcgtttgta
     1681 tttgatttgc tgtgtcatgg gataccttgc tttcttgaat gtcgtcggtc ggagttggga
     1741 cgtctgaatt tcaaatggtc ggattacaat gatatggtgt gtcgttctat tattgttttg
     1801 ttttcagacg gcctttatat tcggtcgtct taaaataggt accgtaaacg gttttctcaa
     1861 aaaaaagcac cctgattcgt ttggaatcca ggtgctttct ttttcataag tgccttacac
     1921 tttggcggct gcggctactt cggctgcgta gtcgacaacg gctttctcga taccgtcgcc
     1981 tactttgtag cgtacgaagc taaccacttc agtgtcgttt tctttagcga attgggcaac
     2041 agtttggtca gggttcatca cgaatgcttg gccgttcaga gtgatttcag ccaagaattt
     2101 gcggatacgg ccttcaacca ttttagcggc gatgtcggca ggtttgccgg aagcgatggc
     2161 ttgctcggtg tagatgtggc gttctttttc aacggtttca gcatctactt cggcttcgct
     2221 tacgcattgc ggtttggcgg caacgatgtg cataccgatt ttgcgtgcta catcttcaga
     2281 gcctttgtac tcgaccaaaa cgccttcggt cgccaatgcg ccgtggatgt aggcaaccag
     2341 ttggttggca gtgtcaatca cttggaagcg gcggacagac atgttctcgc ccaatttagc
     2401 gatgatggct ttgcgttctg attcaaccag ttcgctcagt tcttcaacag aagccggttt
     2461 tttctcggca gcagttttcg caacgaagtt ggcaaattct acgaagccgg cgtctttagc
     2521 aacgaagtcg gtttcgcagt ttacttcgac caatgcgccg acattaccgt tgattgcata
     2581 agccaatacg ccttcggcag cagtacggcc agccagtttg cccgctttcg caccggattt
     2641 aatacgcagg atttcttcgg ctttgtcgaa gttgccttca gcttcaacca aggctttttt
     2701 gcattccatc atacccaggc cggtagcggc gcgcaggtcg gcaaccattt ttgcagtaat
     2761 ttctgccatt ttgaatctcc tagatttttg ggaaatacgg aaaggatttt ccacattcgg
     2821 gaatcgtgtg ccgcgaacgg gcgttcagac ggcatatttg agaaaagggg cataacgccc
     2881 ctcttctgtg taccgaatta ctcggcagcg gtttcttggg cagcggctac ggtttcttgc
     2941 agcgcttggt ttttgccttc caaaactgcg tcggcgatgc ctcggcagta caggcggatg
     3001 gctttggcgg agtcgtcgtt gccggggatg acgtatttca cgccgtctgg gctgttgttg
     3061 gtatcgacta cggcgataac ggggatgccc agtttttcag cttcaaccag agtacctttt
     3121 tggtagccgg tatcgataac gaaaatcgcg tcgggcaggc ctttcatgtt tttgataccg
     3181 cccaaagagc gttccagttt ttcaacgtcg cgttgcattt ccagaatttc ttttttgctg
     3241 aaaccgcttt cggcagcatt ttccaaggct gcggtttttt cttccaggcg tttgatggat
     3301 tgcttaacgg ttttgtagtt ggtcagcata ccgcccaacc agcggtggtc gacgaaaggc
     3361 ataccggcgc gggtcgcttc ttcgcggatg atgtcgcgtg cctggcgttt ggtacctacg
     3421 aacaataccg tacctttgtt ggcaaccaga cgacgtacgg cttcttgcgc ctcttggaac
     3481 atcggcaggg ttttttccaa gtttacgata tggattttgt tgcgcgcgcc gaaaatgtat
     3541 tgagccattt tcgggttcca gaaacgggtt tggtgaccga agtgaacacc ggcttcaatc
     3601 atctgacgca tagtaatttg agacatgtta tttccttgaa agggttaaag agcacacatt
     3661 caatcgcata gaacttgata ggcggcgcct attgtcaagc acccttcggc gaaagtgggc
     3721 ataagtttta aaaatagtgt gtttccgaaa tggaaaactg acggattata gcggataaga
     3781 tgccgtctga aaagggattc agggttttgt gccgctgtct ttttgccgtt gtgcgcgctg
     3841 ctgttcggat tctgcctgtt cctgctgccc catcaggcgg ttgcggcggc ggacggttac
     3901 ggcgaagacc gtgaacacgg tgggcagaac cacccaaaaa accaaataaa tcaaagcgcg
     3961 cgcaatactc ggctgcgcgg cggaatacat aacggcaaca aaaagatagc cgatggcgac
     4021 gatgtgccac attgtgtgcg tcctgtttga atgttaacaa aatcgttata atcgcaacat
     4081 tctaccgcaa tcctgaaagg ccgtctgaaa tgaggaacga ggaaaaacgc gccctgcgcc
     4141 gcgaattgcg cgggcggcgt tcgcaaatgg ggcgggacgt gcgggcggcg gcggcggtaa
     4201 aaatcaaccg cctgctcaaa cgttatatca agcgcggtcg gaaaatcggc gtgtattggc
     4261 cgatgggcaa ggaattgcgt ttgggcggct ttgtccgcgc ggcgcaaaaa cgcggcgcaa
     4321 aactctatct gccttatatc gaaccgcaca cgcggcggat gtggtttacg ccgtatcctg
     4381 aacgcggaat ggaacgggaa cgcaagcgcg gtagggcgaa gctgcatgtc cctcagtttg
     4441 cagggcgcaa aatccgcgtg cacggtttgt cggtattgct cgtcccgctt gtcggcatag
     4501 accgcgaagg ctaccgtttg gggcaggcag gcggctatta cgatgcgacg ctttcggcga
     4561 tgaaataccg tttgcaggcg aaaaccgtgg gcgtgggctt tgcctgccag ttggtggaca
     4621 ggctcccacg cgaggcgcac gacctgccgc tggacggttt tgtatcggaa gcggggatat
     4681 tgtgttttta gccgttcaga cggcatttcg ggcggaaatg ccgtctgaag gcggaatatt
     4741 gggagaaaag gatgtcggaa aggataatac tgccggtttt gtcattgggc ggcgtacggc
     4801 tcgaaccgtt ggatgtgcat catgaaacgg gtttgcgcga ggcggtttgc gatggggagg
     4861 tttggaagct gggggtaacg tccgcgcccc atccggatag ggtggcagac tatatcggga
     4921 cagcattggc aacgcgtttg gcgtttgcgg ttgtcgatga agaggcggac agggtggtcg
     4981 ggacaacggc gtattatcat tttgaaccgc agattccgcg tttggatatc ggattcacat
     5041 ggtatgcggc atcggcacgg cggaaacgga taaacacctg ttgcaaaatc atgctgttgg
     5101 attacgcatt tgatgtttta gcttgctgtt gcgcgggatg gcgcacggat attctgaacc
     5161 ttgcctcgca acgtgccatc gaacggctgg gcgcggaaaa agacggcgtg ctgcgtatgc
     5221 atatgctccg taaagacggc agtgtgcgcg atacggttgt gtacagtatg ctgagggaag
     5281 attggtgcaa aaacagggaa atcctgaccg gcaggttggc agggtatggc gtgcaggtgt
     5341 gaggcagtat tctcaaattt aaaaaactta acgcgatatg ctgac
//
LOCUS       DAWTQS010000092         5281 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.092, whole genome shotgun sequence.
ACCESSION   DAWTQS010000092 DAWTQS010000000
VERSION     DAWTQS010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5281
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.092"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(125..2863)
                     /gene="tbpA"
                     /locus_tag="WNA25_001948"
     CDS             complement(125..2863)
                     /gene="tbpA"
                     /locus_tag="WNA25_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent transferrin-binding protein TbpA"
                     /protein_id="HFA9824624.1"
                     /translation="MQQQHLFRFNILCLSLMTALPAYAENVQAGQAQEKQLDTIQVKA
                     KKQKTRRDNEVTGLGKLVKTADTLSKEQVLDIRDLTRYDPGIAVVEQGRGASSGYSIR
                     GMDKNRVALTVDGLAQIQSYTAQAALGGTRTAGSSGAINEIEYENVKAVEISKGSNSV
                     EQGSGALAGSVAFQTKTADDVIGEGRQWGIQSKTAYSGKNRGLTQSIALAGRIGGAEA
                     LLIRTGRHAGEIRAHEAAGRGVQSFNRLAPVDDASTYAYFIVEEECKNGGYEKCKAKK
                     DVDGKDERQTVSTRDYTGPNRFLADPLSYESRSWLFRPGFRFENKRHYIGGILERTQQ
                     TFDTRDMTVPAFLTKAVFDENKKYGSIRGYGKYAGDHRYGGLITNSENGAQVGAEYGT
                     GVFYDETHTKSRYGLEYVYTNADKDTWADYARLSYDRQGIGLDNHFQQTHCSADGSDK
                     YCRPSADKPSSYYKSDRVIYGESHRLLQAAFKKSFDTAKIRHNLSVNLGYDRFGSDLR
                     HQDYYYQHANRAYSSKTPPQNNGKKTSPNGGKDKPYWVSIGGGNVVTGQICLFGNNTY
                     TDCTPRSINGKSYYAAVRDNVRLGRWADVGAGLRYDYRSTHSDDGSVSTGTHRTLSWN
                     AGIVLKPADWLDLTYRTSTGFRLPSFAEMYGWRSGDKIKAVKIDPEKSFNKEAGIVFK
                     GDFGNLEASWFDNAYRDLIVRGYEVQIKDGKEQVKGDPAYLNAQSARITGINILGKID
                     WNGVWDKLPEGWYSTFAYNRVRVRDIKKRADRTDIQSHLFDAIQPSRYVVGSGYDQPE
                     GKWGVNGMLTYSKAKEITELLGSRALLNGNSRNTKATARRTRPWYIVDVSGYYTVKKH
                     FTLRAGVYNLLNHRYVTWENVRQTAAGAVNQHKNVGVYNRYAAPGRNYTFSLEMKF"
     gene            complement(2950..5031)
                     /locus_tag="WNA25_001949"
     CDS             complement(2950..5031)
                     /locus_tag="WNA25_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFA9824625.1"
                     /translation="MNNPLVNQAAMVLPVFLLSACLGGGGSFDLDSVDTEAPRPAPKY
                     HDVPSKKPEARKDQGGYGFAMRFKRRNWYRATNENEVKLKESDWEQTDDGEIKNPFKQ
                     KNIINALPGNEGELLQDSSQQGKGISKVGDHHDFKYVWSGFFYKRIKITTKKNESNKI
                     IEARSGPDGYIFYKGRNPSRKLPVSGEVTYKGTWDFLTDVKANQRFTDLGNTSTKSGD
                     QYSAFSGELDYIVKKEEDKKEKHKGLGLTTEITVDFGKKILNGKLIKNNYVINNNGDG
                     QNKYTTEYYTLDATLRGNRFSGKATATDKSSNEQAKLHPFVSDSSSLSGGFFGPQGEE
                     LGFRFLSDDNKVAVVGSAKTKDKNANGNTAAAGTAGAAGMSSEDTKLTTVLDAVELKS
                     DGKKVENLDNFSDATRLVVDGIMIPLLPTESGNGQADKGKNGKTAFIYETTYTPESDK
                     KDTKAGTAANGVQTVSNTAGGTSGKTKTYKVQVCCSNLNYLKYGLLTRENNNSVMQAV
                     KNSNRTADRTAQGAQSMFLQGERTDEKEIPKDENVVYLGTWYGHIAANSTSWTGNASD
                     QQSGNRARFDVNFKDKKITGTLTAANRQAETFTISGMIDGNGFEGTAKTGNGGFALDA
                     NNTAATHKAHIAEAKVRGGFYGPNAEELGGWFAYPGNGQAKNAQASSGNENSAGSATV
                     VFGAKRQQLVQ"
BASE COUNT         1070 a         1383 c         1324 g         1504 t
ORIGIN      
        1 cacgcctgcg cgggaatgac gggtttcggg attacggtgt atcgggaggg atggggaacg
       61 gtgaaatttg tgtaaaaaaa cgccgtctga agcctttcag acggcatttg cggcgttcgg
      121 acgtttagaa cttcatttcc aagctaaatg tgtagttgcg gccgggggcg gcatatcggt
      181 tgtaaacgcc gacatttttg tgttggttga ctgcgccggc ggcagtttgc cgcacatttt
      241 cccaagtaac atagcggtgg ttgaggaggt tgtacacgcc cgcacggagg gtgaagtgtt
      301 ttttaaccgt gtaataaccg gacacgtcca caatatacca agggcgggta cggcgcgcgg
      361 tggcttttgt attgcggctg ttgccgttga gcaaagcccg gctgcccaac aactctgtga
      421 tttccttggc tttggaataa gtcagcatac cgttcacacc ccatttgcct tccggttggt
      481 catagcccga gccgacgaca tagcgcgagg gttggatggc atcaaacagg tgtgattgga
      541 tatcggtgcg gtctgcgcgt tttttaatat cgcggacacg gacacgatta taggcaaatg
      601 tggaatacca accttcgggc aatttatccc atacgccgtt ccaatcgatt ttgcccaaaa
      661 tattgatgcc ggtaatccgc gcgctttggg cattgaggta agccgggtcg cctttgactt
      721 gttctttgcc gtctttaatt tgcacttcat aaccccggac aatcaaatcg cggtaggcat
      781 tgtcgaacca acttgcctcc aagttgccga aatcgccttt aaacacgatg ccggcttctt
      841 tgttgaacga tttttccgga tcgattttga cggcttttat tttatcgccc gaccgccagc
      901 cgtacatttc cgcaaacgag ggcaggcgga agccggttga ggtgcggtaa gtcaaatcca
      961 gccagtcggc aggtttgagg acgatgccgg cgttccagga cagggtgcgg tgcgtgccgg
     1021 tggaaacgct gccgtcgtcc gaatgcgtgc tgcggtagtc gtagcgcaag cccgcgccga
     1081 catccgccca cctgcccaaa cggacgttgt cccggaccgc cgcgtaatag cttttgccgt
     1141 tgatgctgcg cggcgtgcag tccgtataag tattgttgcc aaagaggcag atttgccccg
     1201 taaccacatt tcccccgcct atgctgaccc aatagggctt gtccttgccg ccgttggggc
     1261 tggttttttt gccgttgttt tgagggggcg ttttcgacga ataggcgcgg ttggcatgtt
     1321 gataataata atcctgatgg cggaggtcgg aaccaaaacg gtcgtaaccg aggttgacgc
     1381 tcaggttgtg gcggattttg gcggtatcga aggatttttt gaatgccgcc tgcaagagcc
     1441 tgtggctttc cccgtaaatc acgcggtcgg atttgtagta ggaagacggc ttgtcggcac
     1501 tcgggcggca atatttgtcc gaaccgtcgg cggaacagtg cgtctgctga aaatgattgt
     1561 ccaaaccgat gccctgccgg tcgtaagaga ggcgggcata atccgcccaa gtgtctttat
     1621 cggcattggt atagacatat tccaaaccgt agcggctttt ggtgtgcgtc tcgtcgtaaa
     1681 acacgcccgt accgtattcc gcgcccacct gcgcaccgtt ttcactgttg gtaatcagcc
     1741 cgccgtatcg gtggtcgccc gcgtatttgc cgtagcctct tatcgatccg tattttttat
     1801 tttcatcaaa aaccgccttg gtcaggaatg ccggaaccgt catatcgcgc gtgtcgaaag
     1861 tttgttgcgt gcgttcgagt atgccgccga tgtagtgccg cttattctca aaacgaaaac
     1921 ccgggcggaa cagccacgac cggctttcgt atgaaagcgg atcggcgagg aagcggttgg
     1981 ggcccgtgta gtctcgggtg gaaaccgttt gacgttcgtc tttgccgtca acatcttttt
     2041 tcgctttaca cttttcgtaa cccccgtttt tgcattcttc ttcaacgatg aaataggcgt
     2101 atgtgctcgc gtcatcaacc ggcgccagcc tgttaaagct ctgaacgccg cgtccggcgg
     2161 cttcgtgggc gcggatttcc cccgcgtgcc ggccggtgcg gatcagcaaa gcctccgcac
     2221 cgccgatgcg ccccgccagc gcgatggatt gggtaagccc ccggtttttg ccggaatagg
     2281 cggttttact ctgaatgccc cactgcctgc cttccccgat aacatcgtcg gcggttttgg
     2341 tttgaaatgc gaccgagccc gccaatgcgc cgctgccttg ctcgaccgag tttgagccct
     2401 tgctgatttc gacagccttg acgttctcat actcgatttc attgattgcg ccgctgctgc
     2461 ccgccgtcct cgtcccgccc aatgccgcct gcgcggtgta ggactgtatt tgcgccaagc
     2521 cgtccaccgt caaggcgacg cggtttttgt ccataccgcg tatcgagtag cccgagcttg
     2581 cgccgcgccc ctgttcgacg acggcgatgc cggggtcgta acgcgtcagg tcgcggatgt
     2641 cgagtacctg ttccttgctg agggtgtcgg cggttttgac caatttgccc aaaccggtta
     2701 cttcgttatc gcggcgggtt ttctgttttt tggcttttac ctgtatggta tccaactgtt
     2761 tttcctgtgc ttgtccggct tgcacatttt ctgcataagc gggcagcgca gtcattaaag
     2821 acaggcataa aatattgaat cggaacaaat gttgctgttg catagtgttt ccctaccctt
     2881 cgctttcaga cggaatcgga aggaacgatg ccgtccgaag ccttattctc gattgttcgg
     2941 cagccgtgct tattgcacaa gctgttggcg tttcgcaccg aataccacgg tcgcgctgcc
     3001 tgctgaattt tcattgccgg atgaagcttg cgcatttttc gcttgcccat tgcccggata
     3061 ggcaaaccat ccgcccaact cttcggcgtt aggcccgtaa aaaccgcccc gcaccttggc
     3121 ttctgcgatg tgcgccttat gtgtagcagc ggtattgttc gcatccagcg caaagccgcc
     3181 attaccggtt ttcgccgtac cttcaaatcc gttgccatca atcattccac taatggtaaa
     3241 ggtttccgcc tgcctgtttg cagcggttaa cgtgccggta atttttttgt ctttgaaatt
     3301 cacgtcaaac cttgcccgat taccactttg ctgatcggag gcgttgccgg tccagcttgt
     3361 gctgttggcg gcaatatgcc cgtaccaagt gcctagataa acgacgtttt cgtcttttgg
     3421 aatttctttt tcatcggtgc gctcgccttg gaggaacata ctttgtgcgc cctgtgccgt
     3481 tcggtcggca gttcggttac tgtttttgac tgcctgcatc acggaattgt tgttttcacg
     3541 tgtcagcagc ccgtatttca gataattgag gttggaacag cagacttgga ctttataggt
     3601 ttttgttttg ccacttgtgc cgcctgccgt atttgaaacg gtttgcacgc cattcgccgc
     3661 cgtacctgct ttggtgtctt ttttatcact ttccggcgtg taggttgttt cgtagataaa
     3721 ggctgttttg ccgtttttac ctttatctgc ctgaccgttc ccgctttcgg tgggcaggag
     3781 cggaatcata atgccgtcga caaccagtcg ggtagcgtcg ctgaagttgt cgagattttc
     3841 gactttctta ccgtccgatt tcagctcgac cgcatccaaa accgtggtca gcttagtgtc
     3901 ttcagacgac atgcctgccg cacctgcggt gccagccgcc gcagtattgc catttgcgtt
     3961 tttgtctttg gttttcgcgc tgccgacaac ggcaactttg ttgtcgtcgc tcaaaaagcg
     4021 gaaacccaat tcctcaccct gcgggccgaa aaagccgccg ctcaaagaag acgagtcgga
     4081 aacaaaggga tgtagtttgg cttgttcatt gctggattta tcggtcgccg tcgccttgcc
     4141 gctgaagcgg ttgcccctaa gcgtcgcatc aagggtgtag tattcggtgg tatatttatt
     4201 ttgaccatca ccattattat tgattacata attgttttta attaattttc cgttcaggat
     4261 ttttttccca aaatcaaccg ttatttccgt tgttaatccc aaacctttgt gcttttcttt
     4321 tttatcctcc tcttttttga ctatataatc caactccccg gaaaaagcac tatattggtc
     4381 tccggatttc gtagaagtat ttcctaaatc tgtaaatctc tgatttgctt tcacatcagt
     4441 taaaaaatcc caagtacctt tgtacgtaac ctcccctgaa acaggaagtt ttctcgaggg
     4501 atttctgcct ttataaaaaa tataaccgtc aggaccgctt ctggcttcga ttattttatt
     4561 agattcgttt tttttagttg taatcttaat ccgtttataa aaaaaacccg accatacgta
     4621 tttaaaatcg tgatggtccc caaccttaga tataccctta ccttgttgac tggaatcttg
     4681 caataattcc ccctcatttc caggtaaggc attaataata tttttttgtt tgaaagggtt
     4741 tttgatctca ccatcatccg tttgttccca atcactctct ttcagtttaa cctcgttttc
     4801 gtttgtcgcc cgataccaat tccgccgctt gaagcgcatc gcgaaaccgt atccgccttg
     4861 gtcttttcgg gcttccggtt ttttggaagg aacatcgtga tactttggcg cgggacgcgg
     4921 ggcttcggta tcgacagaat caagatcgaa gctgccgccc ccgcccagac aagcgctcaa
     4981 caaaaacaca ggcagcacca tagcagcctg attcaccaat ggattgttca taacaaaccc
     5041 gattcaatta aagaatgata aggattatta ttttatttat ttttaaaaaa tttgcaaatg
     5101 cttttttata tttttagtaa ataaaccaaa ataccgtcat tcccgcgctg cttttaaaac
     5161 cgcctgcaac cgccaaacct gctgcaatcc gctccctccc ctgcgcgggg gagggctggg
     5221 gagagggcat tctccgagtt gcggcaacct ctcccaaatc ccttagtctt ctaaaacaca
     5281 a
//
LOCUS       DAWTQS010000093         5251 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.093, whole genome shotgun sequence.
ACCESSION   DAWTQS010000093 DAWTQS010000000
VERSION     DAWTQS010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.093"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            103..924
                     /locus_tag="WNA25_001950"
     CDS             103..924
                     /locus_tag="WNA25_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HFA9824626.1"
                     /translation="MSSPRQVFYISDRTGLTAENIGEALLNQFGNLSFKRHTHPFVDT
                     PEKARAVVEKVNRSRQENGQRPIAFVSVVDDEIRRIIKGADAFQINFFETFLGLLEKE
                     LNTEATVSGQGHHSIGNTKRYDARMEAVNFSLNHDDGVSDKNLQEADVILMGVSRSGK
                     TPTCLYLALQYGIRAANYPLIPDDLESADLPRMVKPYKDKLFGLTIQPERLQAIRQER
                     RPNSAYARIDTCRSEVADAQSMFRRHGIPFANTTDKSVEELAVHILQACKLKRRF"
     gene            complement(1010..1672)
                     /locus_tag="WNA25_001951"
     CDS             complement(1010..1672)
                     /locus_tag="WNA25_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFA9824627.1"
                     /translation="MIQAVLFDLDGTLADTALDLGGALNTQLARHGLPEKSMDEIRTQ
                     ASHGAAGLLKLGANITPEHPDYTAWRTEYLEEYDSRYAQDTTLFDGVNELIAELGRRG
                     IKWGIITNKPMRFTDKLVPKLGFAVPPATVVSGDTCGEPKPSIKPMLHACGKIHADPQ
                     HTLYVGDAERDIQAGRNAGMKTVLAEWGYISDEDDTGSWQADFHIRTPLDLLECLDKI
                     QP"
     gene            complement(1710..2537)
                     /locus_tag="WNA25_001952"
     CDS             complement(1710..2537)
                     /locus_tag="WNA25_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFA9824628.1"
                     /translation="MNPDIYALLERALLSGDPDEKGRLTDEAFAAVQNADGAEANAPP
                     ADFPRAGRPDTPVLVAPSQLTPRKMNTAEGYAAMLHAITHIEFNAVNLALDAAYRFRT
                     LPFQFVRDWVKVAKEEVYHFRLVRDRLRAFGFDYGSFEAHNHLWDMAYKTAYDPLLRM
                     ALVPRVLEARGLDVTPGIRAKVEQRGDSATCGVLDIIYRDEVGHVAIGNRWYQHLCRE
                     RGLEPVALFRSLIARYDMFIFRGYVNIEAREKAGFSRFELDMLEDFEQGLKQNKHAV"
     gene            2920..3543
                     /gene="lolA"
                     /locus_tag="WNA25_001953"
     CDS             2920..3543
                     /gene="lolA"
                     /locus_tag="WNA25_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFA9824629.1"
                     /translation="MMKPHNLFQFLAVCSLTVAVASAQAGAVDALKQFNNDADGISGS
                     FTQTVQSKKKTQTAHGTFKILRPGLFKWEYTLPYKQTIVGDGQTVWLYDVDLAQVTKS
                     SQDQAIGGSPAAILSNKTALESSYTLKEDGSSNGIDYVRATPKRNNAGYQYIRIGFKG
                     GNLAAMQLKDSFGNQTSISFGGLNTNPQLSRGAFKFTPPKGVDVLSN"
     gene            3816..4951
                     /locus_tag="WNA25_001954"
                     /pseudo
     CDS             3816..4951
                     /locus_tag="WNA25_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709746.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
     gene            5046..>5251
                     /locus_tag="WNA25_001955"
     CDS             5046..>5251
                     /locus_tag="WNA25_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFA9824630.1"
                     /translation="MNLSIVVPIYNVESYLEACLNSIEPILSNENVELILVNDGSKDG
                     SEDICYKYIDKISNTKHQTPNTKH"
BASE COUNT         1235 a         1430 c         1445 g         1141 t
ORIGIN      
        1 ttttttgggg tgtaatccga ggtaggggcg gctggggtgc ttctcccttg tctgccgctg
       61 ctgttatgat gatattttta ttctgtattt aaggaggggg tgatgagcag tccgcgccaa
      121 gtgttttata tttccgaccg caccggtctg actgccgaga atatcggcga ggctttgctg
      181 aaccagtttg gcaatctgtc gttcaaacgc catacgcatc cgtttgtcga tacgccggaa
      241 aaggcgcgcg cggtggtgga gaaggtcaat cggagccggc aggaaaacgg tcagcgtccg
      301 attgcgtttg tcagtgtggt tgatgacgaa atccgccgga ttattaaagg ggcggatgct
      361 tttcagatta atttctttga gacttttttg ggactgttgg agaaggaact caataccgaa
      421 gcaacggtat ccgggcaggg gcatcacagt atcggcaata cgaagcgtta cgatgcgcgt
      481 atggaggcag tcaatttttc tttgaaccat gatgacgggg tcagcgataa gaaccttcag
      541 gaggcggatg tgattctcat gggcgtgtcc cgttcgggca aaacgccgac ctgcctgtat
      601 ctggcgttgc aatacggtat ccgtgcggca aactatccgc tgattcccga cgatttggaa
      661 tcggccgatc tgccgcgtat ggtcaagccg tataaagaca aactgttcgg gttgactatc
      721 cagccggaac gtttgcaggc catccgtcag gaacgccgcc cgaattcggc ttatgcgcgc
      781 atcgacacct gccgcagcga agtggcggac gcgcagagta tgttcagacg gcatggcatt
      841 ccgtttgcga atacgacgga caagtcggtt gaggaactgg cggtacacat ccttcaggcg
      901 tgcaagctca aacgcaggtt ttgacgggct ttgattcggt ttgaaggtgg aaatgccgtc
      961 tgaaatcagg tttcagacgg cagttttatg tttgtggggc ggatattttt cagggctgta
     1021 ttttgtccag acattcgagc agatcgagcg gcgtgcggat gtggaaatcc gcctgccatg
     1081 agccggtatc gtcttcatcg gaaatatagc cccattcggc gaggacggtt ttcataccgg
     1141 cgttgcgtcc ggcttggatg tcgcgttccg cgtcgccgac gtagagggtg tgttgcgggt
     1201 cggcgtggat ttttccgcag gcgtgcagca tgggtttgat gctgggtttg ggttcgccgc
     1261 aggtgtcgcc gctgacgacg gtggcgggcg gaacggcgaa tccgagtttg ggaacgagtt
     1321 tgtcggtgaa gcgcatgggt ttgttggtga tgatgcccca tttgatgccg cgtctgccga
     1381 gttcggcgat gagttcgttc acgccgtcga agagagtggt gtcttgggcg tagcggctgt
     1441 cgtattcctc cagatattcg gtgcgccatg cggtatagtc ggggtgttcg ggcgtgatgt
     1501 tcgcgccgag tttgagcaat cctgccgcgc cgtggctggc ttgggtgcgg atttcgtcca
     1561 tgcttttttc gggcagtccg tggcgggcga gttgggtgtt gagtgcgccg ccgaggtcta
     1621 gggcggtgtc ggcgagcgtg ccgtcgaggt cgaacaatac ggcttgtatc atgtgttttc
     1681 cttttttata aagtgcggga cgaaaggttt cagacggcat gtttattttg tttcaaaccc
     1741 tgctcgaaat cttccaacat atccaattcg aagcggctga agcccgcttt ttcccgcgct
     1801 tcgatgttca catagccgcg gaagataaac atatcgtaac gggcaatcag actgcggaac
     1861 agggcgacag gttccaaacc gcgttcgcgg cagaggtgtt gataccaccg gttgccgatg
     1921 gcgacgtgtc ccacttcgtc gcggtaaatg atgtccaaca cgccgcaggt tgccgaatcg
     1981 ccgcgctgct ccaccttcgc gcgtatcccc ggcgtaacgt ccagtccgcg cgcctccaac
     2041 acgcgcggta ccaacgccat acgcaacagc gggtcgtagg cggttttgta cgccatatcc
     2101 cacaaatggt tgtgcgcctc gaaactgccg taatcgaagc cgaaagcgcg caatctgtcg
     2161 cgcaccaggc ggaagtggta cacctcctcc ttcgccactt tcacccagtc gcggacaaac
     2221 tgaaacggca gcgtgcggaa acggtatgcc gcgtccaaag ccagattgac ggcgttgaac
     2281 tcgatatgcg taatcgcgtg cagcatcgcc gcatagcctt ctgccgtgtt cattttgcgc
     2341 ggggtcagtt gcgagggcgc gaccaaaacc ggcgtgtccg gccgtcccgc gcgggggaag
     2401 tccgccggcg gcgcgtttgc ctccgccccg tccgcatttt gaacggcggc aaacgcctca
     2461 tccgtcagcc gtcctttttc atcggggtcg cccgaaagca gggcgcgttc cagcaaagca
     2521 taaatatcgg ggttcatctc aagtccgccg tgttcggaaa acgaatatta tagcgtttaa
     2581 aaaaaacaag atgaggcata taatctccgc gattcggcat tccgcgccca aaccgtcaaa
     2641 tatagtggat taacaaaaac cagtacggcg ttgccccgcc ttgccgtact atgttgtact
     2701 gtctgcggct tcgtcgcctt gtcctgattt ttgttaatcc actataacgc ggcatacaat
     2761 tgaaagggca gcgtggcgtg cctgcttttt ccgagcggtc aaaaaaatca gccctcggaa
     2821 aacgcggttt gcaaaatgca aaccgcccgt aacgccgccc gtatgattgt tttgccgcgc
     2881 cgatactttg cgccacaccc atcccgacaa ggaaaaataa tgatgaaacc gcacaacctg
     2941 ttccaattcc tcgccgtttg ctccctgacc gtcgccgtcg cttccgcaca ggcgggcgcg
     3001 gtggacgcgc tcaagcaatt caacaacgat gccgacggta tcagcggcag cttcacccaa
     3061 accgtccaaa gcaaaaagaa aacccaaacc gcgcacggca cgttcaaaat cctgcgcccg
     3121 ggcctcttca aatgggaata cactttgccc tacaaacaga ctattgtcgg cgacggtcaa
     3181 accgtttggc tctacgatgt tgatttggca caagtgacca agtcgtccca agaccaggcc
     3241 atcggcggca gccccgccgc catcctgtcg aacaaaaccg ccctcgaaag cagttacacg
     3301 ctgaaagagg acggttcgtc caacggcatc gattatgtgc gggcaacgcc caaacgcaac
     3361 aacgccggct accaatacat ccgcatcggc ttcaaaggcg gcaacctcgc cgccatgcag
     3421 cttaaagaca gcttcggcaa ccaaacctcc atcagtttcg gcggtttgaa taccaatccc
     3481 caactctcgc gcggcgcgtt caagtttacc ccgcccaaag gcgtggacgt gttgagcaac
     3541 tgatgccggc cgccccgatg ccgtctgaac gccgccgagg cttcagacgg cattttcacg
     3601 caggcggaac aatgtcccgc attatcggcc gatcgggcaa cggaacggca aatccgtgaa
     3661 aattaacggt tgcgccccgg ctgtttttgc cgtttaatgc aaaccttgct gcaccaaggg
     3721 ccaagaaagc cgaccggccg cccccacagc ttccgatgca ggcggcccgt ccgtccctgc
     3781 aatgtttttt atttttgaac gaaaggtcga aaaccatgaa aaaaacactg gtggcggcaa
     3841 tcctgagcct tgccttgact gcgtgcggcg gcggaagcga taccgccgcc caaaccccct
     3901 ccgccaagcc cgaagccgaa caatcgggca aactcaacat ctacaactgg tcggattatg
     3961 tcgatcccga aaccgtcgcc gcctttgaaa aagaaaccgg cattaagatg cgttccgact
     4021 attacgacag caacgaaaca ctggaggcaa aagtcctgac cggcaaatcc ggctacgacc
     4081 tgaccgcgcc gtccatcgcc aacgtcggcc ggcaaatcaa agcgggcgcg tatcagaaaa
     4141 tcgacaaggc gcaaatcccc cattacggca acatcgataa agatttgctg aaaatgatgg
     4201 aagccgtcga tccgggcaac gaatacgccg tcccctattt ctggggcatc aacaccttgg
     4261 caatcaacac gcggcaggtg caaaaggcat tgggtacgga caagctgccc gaaaacgagt
     4321 gggatttggt gttcaaaccc gaatacaccg ccaactcaaa tcctgcggca tcagctattt
     4381 cgacagcgca atcgaacaga ttcccttggc gttgcactat ttgggcaaag accccaacag
     4441 tgaaaatccc gaagacatca aagccgccgt cgatatgatg aaagccgtcc gaggcgacgt
     4501 gaaacgcttc agctcttccg gctatatcga cgatatggcg gcgggcaacc tgtgtgccgc
     4561 catcggttac ggcggcgatt tgaacattgc caaaacccgt gccgaagaag ccgcaaacgg
     4621 cgtggaaatc aaagtattga ccccgaaaac cggcgtaggc gtgtgggtgg attcctttat
     4681 gattccgcgc gacgcgcaaa acgttgccaa cgcccaccgc tatatcgact acacgctccg
     4741 gcccgaggtg gctgcgaaaa acggcagctt cgttacctac gcgcccgcca gccgtcccgc
     4801 gcgcgagctg atggatgaaa aatacacctc cgacgcatcg attttcccga ccaaagaact
     4861 gatggaaaaa agtttcatcg tatcacccaa atccgcagaa tccgtcaaac tgggcgtgaa
     4921 gctgtggcaa gggctcaaag cgggcaaata accggaatcc ctgccgtctg aaacctttcg
     4981 ggcggcagga aacggcgtgt ccgcattaag tcaggattag gattatttag aaagatgaga
     5041 tggatatgaa tttaagtatt gtcgttccta tttataatgt cgaaagttat ttggaagcgt
     5101 gtttaaattc tatagaacct atattaagta atgaaaatgt cgaacttatt cttgtgaatg
     5161 acgggtcaaa agacggaagt gaagatatat gttataaata tatagataaa atatcaaaca
     5221 ccaaacacca aacaccaaac accaaacacc a
//
LOCUS       DAWTQS010000094         4849 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.094, whole genome shotgun sequence.
ACCESSION   DAWTQS010000094 DAWTQS010000000
VERSION     DAWTQS010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4849
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.094"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            <1..526
                     /locus_tag="WNA25_001956"
     CDS             <1..526
                     /locus_tag="WNA25_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFA9824631.1"
                     /translation="TAALRAKYGFADHETVFLFPSTGHTRKGLELLADFFEQTELPVK
                     LAVAGSPLPRPMKNVVGLGFCTDMPELYRAADFTIMASLYEPFGLVGVESALCGTRVV
                     LSENMACTEVMNEEAGFFFSRQNPETLAQAVAQAVSLKKQGGHRLSDPMRALNYNPSL
                     SHHIDRLTDMLASV"
     gene            complement(593..2245)
                     /locus_tag="WNA25_001957"
     CDS             complement(593..2245)
                     /locus_tag="WNA25_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFA9824632.1"
                     /translation="MVAYAFLFLFVTAVVLLIVRSHYRWTYFFASALFVFLAGGMLML
                     TAQWQRALNFASVWFVVLILFHRLKIHYYKQPLLISDFLLIADWRNWETLFHYKEAVI
                     GMAGLLALAAYAVFGWSGADSLGMPWRWAGAVLFAAAFVSVRHFSKHPGAVKTWLDSL
                     PDDGRDVFLNLPMSCRAVFFQVPVFEGDGEAFARQMPSETQPYGMSDEKPDIVVTLME
                     STLDPHCFDFAAAKIPDLKMFGRQEDTVFSSPLRVHTFGGATWKSEFAFLAGVPSTDF
                     GALASGVFYSVVPHLQTGFVRNLREHGYFCVALSPFTKGNYNAKAAYDHFGFNLMFQP
                     QDLGYPAPMGKNLWHISSEEMMQYARMILEKRHPDLENVRQPMFVYVLTMKEHGPYRT
                     DTDNVFDLDAPDLNAKTVSALNDYIGRIADLDKAVESFDRYLHERGKPFVFGYFGDHQ
                     VPFEGVSVRKKWDYAQPDYVTQFAVRSNIAGGFVQRQDFLDLAFAGGVLMEAAGLEAK
                     DGFMRANMAMRGLCGGGLEDCPNRELVGNYRNYLYDVLKIAR"
     gene            2388..2951
                     /gene="gmhB"
                     /locus_tag="WNA25_001958"
     CDS             2388..2951
                     /gene="gmhB"
                     /locus_tag="WNA25_001958"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFA9824633.1"
                     /translation="MKLIILDRDGVINRDRDDFVKSVDEWIPVEGSMDAVAFLTQAGC
                     TVAVATNQSGIGRKYFTVQDLTEMHAKMHRLIRQAGGEIDGIWFCPHTGADGCNCRKP
                     KPGMIEDILERFNAQASETWLVGDSLRDLQAIDAVGGKPALVLTGKGKKTLSQHGHEL
                     PGHTQVFDTLLDFSQYIMQENAAPQAD"
     gene            2981..3724
                     /locus_tag="WNA25_001959"
     CDS             2981..3724
                     /locus_tag="WNA25_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HFA9824634.1"
                     /translation="MLIIRNLIYWLILCSSLIFLFPFMLLASPFRDGAHKMARVWVGI
                     LNWSLKHIVGLKYRIIGAEHIPDRPSVICAKHQSGWETLALQEIFPPQVYVAKRELFK
                     IPFFGWGLKLVKTIGIDRNNRREANEQLIKQGLARKNEGYWITIFPEGTRLAPGKRGK
                     YKLGGARMAKMFEMDIVPVALNSGEFWPKNSFLKYPGEITVIICPTIPHASGSEAELM
                     EKCEHLIETQQPLISGAGPFAAEMPSETA"
     gene            3721..4410
                     /locus_tag="WNA25_001960"
     CDS             3721..4410
                     /locus_tag="WNA25_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFA9824635.1"
                     /translation="MTSLIHTLSDGIELTVEINRRAKKNLIIRPIGTHTVRISVPPCF
                     SVSALNRWLYENEAVLRRTLAKTPPHNTANRLPEHIWFHGRQLALTAHQDTQILLMPS
                     EILVPEGAPEKQLALLRDFLERQAHSYLIPRLEHHARATQLFPASSSLTSAKTFWGVC
                     RKTTGIRLNRRLVGAPEYVADYVCIHELCHLAHPDHSPAFWELTRRFAPYTPEAKQRI
                     KIHGRELFALG"
     gene            4492..4623
                     /locus_tag="WNA25_001961"
     CDS             4492..4623
                     /locus_tag="WNA25_001961"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFA9824636.1"
                     /translation="MPPVRGRMAASAPSAPFHFTPPFRNPPDPTCGMKRLSRREVFY"
BASE COUNT         1182 a         1580 c         1171 g          916 t
ORIGIN      
        1 aactgccgcc ctgcgcgcca aatacggttt tgccgaccat gaaaccgttt tcctattccc
       61 atcgaccggc cacacgcgca aaggcctgga actgctcgcc gactttttcg aacaaaccga
      121 actgcccgtc aagctcgccg ttgccggttc accgcttccc cgcccgatga aaaacgtcgt
      181 cggactgggc ttctgcaccg atatgcccga actctaccgc gccgccgact ttaccattat
      241 ggcatccctg tacgaaccct tcgggctggt cggtgtcgaa tccgccctat gcggcacacg
      301 cgtcgtcctc tccgaaaaca tggcatgtac agaggtcatg aacgaagaag ccggattctt
      361 tttctcacga caaaacccgg aaaccctcgc gcaagccgtt gcccaagccg tcagccttaa
      421 aaaacagggc ggacaccgcc tgtccgaccc gatgcgggcg ctgaactaca acccgtccct
      481 gtcgcaccac atcgaccgac tgaccgacat gctggcatcc gtataacaaa atgccgtctg
      541 aacgggtttt cagacggcat tttcgcaacc gttgcagccg tgcggcaaaa agctaacggg
      601 caattttcaa aacgtcgtac agatagttgc ggtaatttcc gaccaactcc cggttcgggc
      661 agtcttccaa ccctccgccg cacaaaccgc gcatcgccat attcgcacgc atgaagccgt
      721 ctttggcttc caaaccagcc gcctccatca gtacgccgcc tgcaaaggca aggtcgagga
      781 aatcctgccg ctgtacgaat ccgccggcaa tattgctcct gacggcaaac tgcgttacat
      841 aatccggctg agcgtaatcc catttcttcc tgacggacac gccctcaaac ggtacctgat
      901 gatcgccgaa ataaccgaaa acaaagggtt tgccgcgttc gtgcagataa cggtcgaaac
      961 tttccaccgc tttgtccaaa tcggcaatgc gcccgatgta gtcgttaagc gcggatacgg
     1021 ttttcgcatt caaatcgggc gcatccaaat caaacacatt gtcggtatcc gtccgatacg
     1081 gcccgtgctc cttcatggtc agcacatata cgaacatcgg ctgccgcacg ttttccaaat
     1141 cgggatggcg tttttcgaga atcatccgcg catactgcat catttcctca ctggaaatgt
     1201 gccacaggtt tttgcccatc ggcgcgggat agcccaaatc ttgcggctgg aacatcagat
     1261 taaagccgaa atggtcatat gctgccttgg cattgtagtt gcccttggta aacggcgaga
     1321 gcgccacgca aaaataaccg tgttcgcgca ggttgcggac aaaaccggtc tgcaaatgcg
     1381 gtacgaccga ataaaacacg ccgcttgcca acgcgccgaa atctgtggac ggaacacccg
     1441 ccaaaaacgc aaattcggac ttccacgttg cgccgccgaa agtatgcacg cgcaaaggtg
     1501 aggaaaatac agtatcttcc tgccgtccga acattttcaa atcgggaatt ttggcggcgg
     1561 caaaatcgaa acagtgcgga tcgagcgtcg attccatcag ggtaacgaca atatcgggct
     1621 tttcatccga catgccgtac ggctgggttt cagacggcat ctgcctggca aacgcttccc
     1681 cgtcgccctc gaacacgggt acttggaaaa acaccgcccg acaggacatc ggcaggttca
     1741 aaaacacgtc gcgcccgtcg tccggcagcg agtccagcca cgtctttacc gcgccggggt
     1801 gcttggaaaa atgccgcacc gacacgaacg ccgccgcaaa cagaaccgcg cccgcccaac
     1861 gccacggcat acccaaagaa tccgcaccgc tccagccgaa aaccgcatat gccgccaaag
     1921 ccagcagccc cgccataccg ataaccgctt ccttataatg aaacagcgtt tcccaattcc
     1981 gccagtcggc aatcagcaaa aagtcggaaa tcaacagcgg ctgtttgtaa taatggattt
     2041 tcagcctgtg gaacagtatc agcaccacaa accagaccga agcgaaattc aaggcgcgct
     2101 gccactgcgc cgtcaacatc agcataccgc ccgccaaaaa gacaaacagc gccgacgcga
     2161 aaaaatacgt ccagcggtag tgcgacctga caatcagcag caccaccgcc gttacaaaca
     2221 aaaataggaa agcataggca accatcccgc cccactcctc aaaacggcat acctgtgccg
     2281 ttgcgaatta ttatcgaaag cgtgcattat aatgcagcac aggcgcattt ccaccgatat
     2341 ttcagtataa tgccatcccc gaacccgccc aacccaaagg aaccgtgatg aaactcatca
     2401 ttctcgaccg cgacggcgtc atcaaccggg accgcgacga cttcgtcaaa tccgttgacg
     2461 agtggatacc tgtcgaaggc agcatggatg cggtggcatt cctgacgcag gcaggctgca
     2521 ccgttgccgt cgccaccaac caatccggca tcggccgcaa atattttacc gttcaagacc
     2581 tcaccgaaat gcatgccaaa atgcaccgcc tcatccgtca ggcgggcggg gaaatcgacg
     2641 gcatctggtt ctgcccgcat accggcgccg acggctgcaa ctgccgcaag cccaaaccgg
     2701 gcatgattga agacatcctc gaacgcttca acgcccaagc ttcggaaact tggctggtcg
     2761 gcgacagcct gcgcgatttg caggcaatcg atgccgtcgg cggaaaaccc gcgctggttc
     2821 tgaccggaaa aggcaaaaaa acgctctccc aacacggaca cgaattaccc ggacacacac
     2881 aggttttcga taccctgctc gatttctcac aatacatcat gcaggaaaac gccgcaccgc
     2941 aagccgactg aacataccgc atttccgaca aggcaaaacc atgctcatca tccgcaacct
     3001 gatttactgg ctgatactct gttccagcct gattttcctc tttcccttta tgctgctcgc
     3061 ctcgcctttc cgggacgggg cgcacaagat ggcgcgggtc tgggtcggca tcctcaactg
     3121 gtcgctcaaa cacatcgtcg ggctcaaata ccgcatcatc ggcgcggaac acattccgga
     3181 ccgcccctcc gtcatctgcg ccaaacacca aagcggctgg gaaacgctcg cgctccaaga
     3241 gatttttccg ccgcaggttt acgttgccaa gcgcgagttg ttcaaaatcc cctttttcgg
     3301 ctggggcttg aaactggtca aaaccatagg catagaccgc aacaaccgcc gcgaagccaa
     3361 cgaacagctc ataaaacagg gtttggcgcg caaaaacgaa ggttattgga ttaccatttt
     3421 ccccgaaggc acgcgccttg cgcccggaaa acgcggcaaa tacaaactcg gcggcgcgcg
     3481 catggcgaaa atgtttgaga tggacatcgt ccccgtcgcc ctcaacagcg gcgaattttg
     3541 gccgaaaaat tcctttctga aatatccggg ggaaatcacc gtcatcatct gtccgaccat
     3601 cccgcacgca agcggcagcg aagccgaatt gatggaaaaa tgcgaacacc tcatcgaaac
     3661 gcagcagccg ctcatttccg gcgcaggccc gtttgccgcc gaaatgccgt ctgaaaccgc
     3721 atgacctccc tgattcacac cctttcagac ggcatagaac tgaccgtcga aatcaaccgc
     3781 cgtgccaaga aaaacctgat tatccgccct atcggcacac ataccgtccg catcagcgtc
     3841 ccaccctgct tctccgtctc cgccctcaac cgctggctgt atgaaaacga agccgtcctg
     3901 cggcggacac tggcgaaaac accgccgcac aatacggcaa accgactgcc cgaacatatc
     3961 tggttccacg gcaggcagct tgcccttacc gcccatcaag acacgcaaat cctgctgatg
     4021 ccgtctgaaa tcctcgtccc cgaaggcgcg cccgaaaaac agcttgcgct gctgcgggac
     4081 tttttggaac ggcaggcgca cagttacctg attccccgtc tcgaacacca cgcccgcgcc
     4141 acgcaactgt tccccgcctc ctcctcgctg acctccgcca aaaccttctg gggcgtgtgc
     4201 cgcaaaacca caggcatacg cctcaaccgg cggctggtcg gcgcacccga atacgttgcc
     4261 gactatgtct gcatacacga actctgccac ctcgcccatc ccgaccacag ccccgccttt
     4321 tgggaactga cccgccgctt cgccccctac acgcccgaag caaaacagcg gatcaaaatc
     4381 cacggcaggg agcttttcgc cttgggctga cgcagccccg ggccgacgct ttcagacggc
     4441 atccgtgccg gaacaggcac gcgcccgccc gattcaaacc gcgatgacgc tttgccgccg
     4501 gttcggggca ggatggcggc atccgcgccg tccgcaccgt ttcatttcac accgcccttc
     4561 cgaaacccgc ccgatccgac gtgcggtatg aaacgcttga gccggcgcga agtcttctac
     4621 tgatttgccc gcgaaaatac cgtccgaaag gttttcggac ggcatttttt tgcgttttcc
     4681 tggatggcgg cggattcgta aaaggcgggc agggtggatt gcaggatggg ttgaggcctg
     4741 ccaaatccgc caactctacc gaatacgccg ccgtcattcc cgcgcaggcg ggaatttggt
     4801 tttgtcggtg cggaaactta tcggataaaa cggtttctta gattccacg
//
LOCUS       DAWTQS010000095         4844 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.095, whole genome shotgun sequence.
ACCESSION   DAWTQS010000095 DAWTQS010000000
VERSION     DAWTQS010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4844
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.095"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            252..1286
                     /gene="pyrC"
                     /locus_tag="WNA25_001962"
     CDS             252..1286
                     /gene="pyrC"
                     /locus_tag="WNA25_001962"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFA9824637.1"
                     /translation="MQTLTIIRPDDMHLHLRDGDALKAVAPYTARQMGRAVIMPNLKP
                     PVVSVADALAYKARIMAALPEGSAFEPLMTLYLTDQATPELVREAKAAGIVAFKLYPA
                     GATTNSDSGVTDLFKLIPVLEEMAKQGILFLVHGEVTDPEIDIFDREAAFIGRVMKPV
                     LAQVPNLKVVFEHITTAEAARLVLEAGDNVAASVTPQHLLLNRNDLLVGGVRPHHFCL
                     PVLKRETHRQALVAAVTGEKAHKFFLGTDSAPHAKSAKENACGCAGMFSAMTAIELYA
                     EVFEKAGALDKLEAFASENGARFYGIPENADTITLVKQSQTVPASVPYGDGELVPMRA
                     GGEIGWTVQY"
     gene            complement(1365..1790)
                     /gene="nusB"
                     /locus_tag="WNA25_001963"
     CDS             complement(1365..1790)
                     /gene="nusB"
                     /locus_tag="WNA25_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFA9824638.1"
                     /translation="MKTARRRSRELAVQAVYQSLINRTAAPEIAKNIREMSDFAKADE
                     ELFNKLFFGTQTNAADYIQKIRPLLDRDEKDLNPIERAVLLTACHELSAMPETPYPVI
                     INEAIEVTKTFGGTDGHKFVNGILDKLAAQIRPDEPKRR"
     gene            complement(1868..2344)
                     /locus_tag="WNA25_001964"
     CDS             complement(1868..2344)
                     /locus_tag="WNA25_001964"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFA9824639.1"
                     /translation="MNTIAPNLDGKHLRIGIVQARFTNEIGSQMLKVCCRTLQELGVA
                     DENITVATVPGALEIPIALMNFASSEKFDALIAIGVVIRGETYHFELVANESGAGIGR
                     VALDYNIPIANAVLTTENDAQAIERIGEKASDAAKVAVECANLVNLLLEEQFEDEE"
     gene            complement(2391..2714)
                     /locus_tag="WNA25_001965"
     CDS             complement(2391..2714)
                     /locus_tag="WNA25_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HFA9824640.1"
                     /translation="MNAFAQALSSALDRCIATNTVAKQNRPVGFLYREAPVFENDSGW
                     RFFSGDETDEYTDDPDNFSIVSLADITKTNPETAALLSQPEGSAWELAEDGTFQTVAD
                     WQPQD"
     gene            2882..3601
                     /gene="rnc"
                     /locus_tag="WNA25_001966"
     CDS             2882..3601
                     /gene="rnc"
                     /locus_tag="WNA25_001966"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFA9824641.1"
                     /translation="MKDDVLKRQAHTAIQKKLGYAFRDMSLLRRALTHRSHHAKHNER
                     FEFVGDSILNYTVARMLFDAFPKLTEGELSRLRASLVNEGVLAEMAAEMNVGDGLYLG
                     AGELKSGGFRRPSILADAMEAMFAAVSFDADFNTAEKVVRHLFAERVRRADFQNQAKD
                     GKTALQEALQARRFALPKYRIEEQIGHANDSMFVISCDLGELGFVCRAKGTSRKAAEQ
                     EAAKEALKWLEEKLPLKKKKK"
     gene            3648..4571
                     /gene="era"
                     /locus_tag="WNA25_001967"
     CDS             3648..4571
                     /gene="era"
                     /locus_tag="WNA25_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFA9824642.1"
                     /translation="MDIETFLAGERAAGGYRCGFVAIVGRPNVGKSTLMNHLIGQKIS
                     ITSKKAQTTRNRVTGIYTDDTAQFVFVDTPGFQTDHRNALNDRLNQNVTEALGGVDVV
                     VFVVEAMRLTDADRVVLKQLPKHTPVILVINKIDKDKAKDRYALEAFVAQVRAEFEFA
                     AAEAVSAKHGLRIANLLELLKPYLPESVPMYPEDMVTDKSARFLAMEIVREKLFRYLG
                     EELPYAMNVEVEQFEEGDGLNRIYIAVLVDKESQKAILIGKGGERLKKISTEARLDME
                     KLFDNKVFLKVWVKVKSGWADDIRFLRELGL"
     gene            4724..>4844
                     /locus_tag="WNA25_001968"
     CDS             4724..>4844
                     /locus_tag="WNA25_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin-binding protein"
                     /protein_id="HFA9824643.1"
                     /translation="MRKLNYYGIALLPLMLASCGGNFGVQPVVESTPTAYPVTF"
BASE COUNT         1042 a         1206 c         1481 g         1115 t
ORIGIN      
        1 aattgagggc ggaacagtcg gtgcaggcac cgtttgaaac caggcaaatc agctatgcct
       61 ggcgggaatc gggcagccct gaagaaaccg tattgaccgc acaaaccgcc gattccccgg
      121 atttacccgt acaggcagta ttgaacctaa cgcaccgttc ccataacggg aaacccctaa
      181 ttctcccctg acttcagacg gcataaagtc ggcatgccgt ctggaaacca aaaatctaaa
      241 aaggaacaac catgcaaacc ctgaccatca tccgacccga cgatatgcac ctgcacctgc
      301 gcgacggcga cgcgctcaaa gccgttgccc cttataccgc ccgccagatg gggcgcgccg
      361 tcatcatgcc caacctcaaa ccgccagtcg tcagcgtagc cgacgcgctt gcctacaaag
      421 cgcgcattat ggcggcgttg cccgaaggca gcgcgtttga gccgctgatg acgctttacc
      481 tgaccgacca agccacgccc gaactcgtgc gcgaagccaa agccgccggc atcgtcgcct
      541 tcaaactcta ccccgcaggc gcgaccacca attccgattc cggcgtaacc gacctgttca
      601 agctcatccc cgtgttggaa gaaatggcaa aacagggcat cctgttcctc gttcacggcg
      661 aagtaaccga ccccgaaatc gacattttcg accgcgaagc cgcctttatc gggcgcgtga
      721 tgaaacccgt tttggcgcaa gtgccgaatc tcaaagtcgt gttcgaacac atcaccaccg
      781 ccgaagccgc ccgcctcgtt ttggaagcgg gcgacaacgt tgccgcctcc gtgaccccgc
      841 aacacctcct gctcaaccgc aacgacctct tggtcggcgg tgtgcgcccc catcatttct
      901 gcctgcccgt cctcaagcgc gaaacccacc gtcaggcatt ggtcgccgcc gttaccggcg
      961 agaaggcgca caaattcttc ctcggcaccg actccgcgcc gcacgccaaa tccgccaaag
     1021 aaaacgcctg cggctgcgcc ggtatgttca gcgcgatgac cgctatcgag ctttacgccg
     1081 aagtgtttga aaaagcaggc gcgttggaca aactcgaagc cttcgcctcg gaaaacggcg
     1141 caaggttcta cggcattccg gaaaacgccg acacgattac ccttgtcaaa caaagccaaa
     1201 ccgttcccgc aagcgtccct tacggcgacg gcgaacttgt cccgatgcgc gcgggcggcg
     1261 aaatcggctg gacggtgcag tattgattga acggtcaagc aaaatgccgt ctgaaaggtt
     1321 ttcagacggc atttgtgtat tttccgattc ggattttccg agtatcaacg gcgtttgggt
     1381 tcgtctgggc ggatttgggc ggcgagtttg tcgaggatgc cgttgacgaa tttgtgcccg
     1441 tccgtgccgc cgaaggtttt ggtaacttcg atggcttcgt tgataatgac ggggtagggc
     1501 gtttcgggca tagcggaaag ctcgtggcag gcggtcagca aaacggcgcg ttcgatgggg
     1561 ttgaggtctt tttcgtccct gtcgagcagc gggcggattt tttggatgta gtccgctgcg
     1621 ttggtttgtg tgccgaagaa gagtttgttg aacaattctt cgtccgcttt ggcaaagtcg
     1681 gacatttcgc ggatgttttt agcaatttcg ggcgcggcgg tgcggttgat aagggattgg
     1741 taaacggctt gtacggcaag ctcgcgggaa cggcggcggg ctgttttcat gatttttcct
     1801 tgaaacggtt gggcggcacg gtatgccgtc tgaaacggaa agggcgcatc ggtgtacgcc
     1861 ctgtttgtta ttcttcgtct tcaaactgtt cttcgagcag aaggttgacg aggttggcgc
     1921 attctacggc gactttggcg gcatccgagg ctttttctcc aatccgttca attgcctgcg
     1981 cgtcgttttc ggtggtcagg acggcgttgg caatcgggat gttgtagtcg agtgcgacgc
     2041 ggccgatccc tgcgccggat tcgttggcaa ccagctcgaa atggtaggtt tcgccacgga
     2101 tgacgacgcc gatggcaatc agtgcgtcaa atttttcgga agaggcaaag ttcatcagcg
     2161 cgatggggat ttcaagcgcg ccgggtacgg tggcgacggt gatgttttcg tctgctacgc
     2221 ccaattcttg gagggtgcgg cagcagactt tgagcatttg gctgccgatt tcgttggtga
     2281 agcgtgcctg tacgatgccg atgcggaggt gtttgccgtc gaggttgggg gcgatggtgt
     2341 tcattgggtg tcctttggta ttcggggttt cggaatgccg tctgaaggtt tcagtcttgc
     2401 ggctgccagt cggcaacggt ttggaatgtg ccgtcttcgg caagctccca cgcgctgcct
     2461 tcgggttggg aaagcagtgc ggcggtttca gggttggttt tggtgatgtc ggcgaggctg
     2521 acgatgctga agttgtcggg gtcgtcggtg tattcgtcgg tttcgtcgcc gctgaagaaa
     2581 cgccagccgc tgtcgttttc aaaaacgggg gcttcgcggt aaaggaagcc gacgggccgg
     2641 ttttgtttgg cgacggtgtt ggtggcgatg cagcggtcga gtgccgagga aagtgcttgt
     2701 gcaaatgcgt tcattgcggg aatacgttgg gggggaaact tgcggatttt accacgattc
     2761 ccgcgttgtc ggcagacggc ggcggtttgg tggtacaatg tgcgccgttt gcagccttaa
     2821 ggtgtttctg tatttttgga gtatggaaac gcattcgggc tgttttttgc ggaagacggt
     2881 aatgaaagac gatgttttga aacggcaggc acatactgcg atacagaaaa agttaggcta
     2941 cgcgttccgc gatatgtcgc ttttgcggcg ggctttgacc cacaggagcc atcatgcgaa
     3001 gcacaacgaa cggttcgagt ttgtcggcga ttcgattttg aattatacgg tggcgcggat
     3061 gctgtttgac gcgtttccga agttgaccga gggcgagttg tcgcggttga gggcgagtct
     3121 ggtcaatgag ggcgtgctgg cggaaatggc ggcggaaatg aatgtcggcg acggtctgta
     3181 tttgggggcg ggcgagttga agagcggcgg cttcagacgg ccttcgatac tggcggacgc
     3241 gatggaggcg atgtttgccg ccgtcagctt cgatgccgat ttcaacacgg cggaaaaggt
     3301 ggtgcgccat ttgtttgcag aacgcgtccg gcgcgccgat ttccaaaatc aggcaaaaga
     3361 cggcaaaact gctttgcagg aggcgttgca ggcgcgccgt ttcgccttgc cgaaataccg
     3421 catcgaagag caaatcggcc atgccaacga cagtatgttt gtcatttcct gcgatttggg
     3481 cgaactgggt ttcgtgtgcc gtgccaaagg gacgagccgc aaggcggcgg aacaggaggc
     3541 ggcgaaagag gctttgaaat ggctggaaga gaagctgccg ctgaagaaga aaaagaaatg
     3601 aggcggcgcg tgaatatgcc gtctgaaatg tggatatgaa agcgaatatg gatattgaaa
     3661 ccttccttgc aggggaacgc gccgccggcg gataccgttg cggcttcgtg gcgattgtcg
     3721 gtcgtccgaa cgtgggcaaa tcaacgctga tgaaccatct catcggtcag aaaatcagta
     3781 ttaccagcaa aaaggcgcag acgacgcgca accgcgtaac ggggatttat accgacgata
     3841 ccgcgcagtt cgtgtttgtc gatacgccgg gctttcaaac cgaccaccgc aacgcgctca
     3901 acgacaggct gaatcaaaat gttaccgagg cgctcggcgg tgtggatgtg gtggttttcg
     3961 tcgtggaggc gatgcgcctt accgatgccg accgcgtcgt gttgaaacaa ctgcccaagc
     4021 acacgccggt cattttagtg atcaacaaaa tcgacaagga caaggcgaaa gaccgttacg
     4081 cgctggaggc gtttgttgcc caagtgcgcg ccgaatttga atttgcggcg gcggaggcgg
     4141 tcagtgcgaa acacggtttg cggattgcca acctgttgga gctgctcaag ccgtatctgc
     4201 ccgaaagcgt accgatgtat cccgaagaca tggttacgga caaatcggcg cgttttttgg
     4261 cgatggaaat cgtgcgtgaa aaactcttcc gctatttggg cgaggagctg ccttatgcga
     4321 tgaacgtcga agtggagcag tttgaagagg gagacggttt gaaccgcatc tacatcgccg
     4381 ttttggtcga caaagaaagc caaaaggcga ttttgatcgg taaaggcggg gagcgtttga
     4441 aaaaaatttc caccgaagcg cggctggata tggaaaaact gtttgataac aaagtatttt
     4501 tgaaggtctg ggtcaaagtc aaatccggtt gggcagacga cattcgcttc ctgcgcgagc
     4561 tgggtttgta gtttttcttg ctgaacttta cgcaaatgcc gtctgaacag gtttcggacg
     4621 gcattttgtt tcaatcggga atatctttgt taaaaacggg ttgatattat ctgtgcatat
     4681 taatataatg ataattatta ttaattaaat aggggggata gggatgcgta aactgaatta
     4741 ttacggtatt gccttgctgc cgttgatgct ggcatcctgc ggcggcaatt tcggcgtgca
     4801 gcctgttgtc gaatcaacgc cgaccgcgta ccccgtcact ttca
//
LOCUS       DAWTQS010000096         4694 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.096, whole genome shotgun sequence.
ACCESSION   DAWTQS010000096 DAWTQS010000000
VERSION     DAWTQS010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4694
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.096"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(1..226)
                     /locus_tag="WNA25_001969"
                     /pseudo
     CDS             complement(1..226)
                     /locus_tag="WNA25_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504043.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Opacity protein opA54"
     gene            659..1579
                     /gene="ribF"
                     /locus_tag="WNA25_001970"
     CDS             659..1579
                     /gene="ribF"
                     /locus_tag="WNA25_001970"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFA9824644.1"
                     /translation="MKIRPGRHNAPDFPHGAAVTIGNFDGVHLGHKHILQKLRLEADA
                     RGLPVVAVIFEPQPKEFFAARTGKTPPCRISPLRTKLGLLEKTGCVDAAWVLRFDRNF
                     SEISAQAFIDRLLRQTLNTRYLLVGDDFRFGAGREGCFELLAQQPDMQTERTPSVIVE
                     DIRTSSTAVRQALSDGNLAYAKKLLGHDYVLGGRVVHGRKLGRTLNAPTANIRLPGHR
                     YALGGVFVVEADGAFGTRRGVASFGFNPTVDGGCSQKLEVHLFDFQGDLYGQRLNVRF
                     LHKLRDEEKFDGMEELKRRIEADMEAAKCW"
     gene            1721..4510
                     /gene="ileS"
                     /locus_tag="WNA25_001971"
     CDS             1721..4510
                     /gene="ileS"
                     /locus_tag="WNA25_001971"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFA9824645.1"
                     /translation="MTDYSKTVNLLESPFPMRGNLAKREPAWLKSWYEQKRYQKLREI
                     AKGRPKFILHDGPPYANGDIHIGHAVNKILKDIIIRSKTQAGFDAPYVPGWDCHGLPI
                     EVMVEKLHGKDMPKARFRELCREYAAEQIARQKKDFIRLGVLGDWDNPYLTMDFKTEA
                     DTVRMLGEIYKSGYLYRGAKPVQFCLDCGSSLAEAEVEYKDKVSPAIDVAYPFKNTVA
                     LAAAFGLAGIEGKAFAVIWTTTPWTLPASQAVSAGADVVYQLIDTPKGKLVLAKDLAE
                     GALKRYGFSDGIAILAETTGDKLENLHMNHPFLERDIPMLNGEHVTTDAGTGLVHTAP
                     AHGLEDYAVCNKYGIELYNPVNAEGKYISETPRVAGMSVWEANPVILQWPEETGNLLA
                     SSKIEHSYAHCWRHKTPLIYRATGQWFVGMDKAGSDGKTLRDKAIKAVDDTEFFPPWG
                     RARLESMIEGRPDWVVSRQRYWGTPMTFFVHKETGELHPNSAELLEKVAQRIEEKGIE
                     AWFSLDKSELLSAEDCEHYDKLPDTMDVWFDSGSTHYSVVKQREELEWPADLYLEGSD
                     QHRGWFQSSMLTGCASSMGRAPYKQLLTHGFVVDQNGRKMSKSIGNVVAPQEVYNEFG
                     ADILRLWAASTDYSGELAISKEILKRVTESYRRIRNTLSFLFANLSDFNPIEDAVQQA
                     DMVEIDRYALVLARRLQERLAGGYYPRYAFHFAVKDIVSFCSEDLGAFYLDILKDRLY
                     TTKADSRARRSAQTALYHITRSLVLLIAPILCFTGEEAWDIIGGGEEDSVLFHTWHEF
                     PAINEKAEAELVKKWTAIREAREAVTAAIEPLRADKTVGSSLQAEAEITAPEEMAGYL
                     NALGEELRFALLVSKAEVKVGDELAVAAKASDGEKCERCWHYTRDVGAVAGYETVCKR
                     CAENVGGEGETRHYA"
BASE COUNT         1126 a         1299 c         1268 g         1001 t
ORIGIN      
        1 cgaagtcgta gccgaccgac accctggggt ggatggaatg tgtacggatg tttctgaaat
       61 aatcgcttac cgtgcttatt tttttgcctt tttctagacc ggctgcatcg ggataatcgc
      121 gggtgatgtg ctcgtaggcg taggctaaat ccgcctgcac atacgggccg cggccattgc
      181 cttcacttgc cgcctgcgct gcggaagaga agagaagaga agagaaggtt ttttgcgggc
      241 tggattcatt ttcggctcct tatatcggtt tgaccggtta aaaaaaagat tttcactgat
      301 gcttcaaagg cggattatat cgggttccgg gcggtgtttc aatacatagc accgcgcctg
      361 ctgcgcgttt tatgcgtttg gcgcgttcgg cggcgggaaa tttgcctact tttcccgcgt
      421 cgggcgggcg gaacgggcgg cacactgtct ataaaccgca ataccgttta caatgaccgc
      481 ctgtttcacc acatacccga acgcaacaat gtttcaacac acgggacggc acatcaagca
      541 ccgccctatg tgtcgtcctg atttggaagg ggttgcaccc ctcccgaata aagtctgatc
      601 ctgccgcccc gaaggacgga tgtccgagtg gcggggtttc aaccgaaaag gaaatacaat
      661 gaaaatcagg ccggggcggc acaacgcgcc cgactttccg cacggggccg ccgtaaccat
      721 aggcaatttc gacggcgtgc acctcggaca caaacacatc ctccaaaaac tccgcctcga
      781 agccgacgca cgcggattgc ccgtcgttgc cgtcattttc gagccgcaac ccaaagaatt
      841 ttttgccgcc cgcacaggca aaaccccgcc gtgccgtatc agccccctgc gtaccaaact
      901 cgggctgctc gaaaaaaccg gttgcgtcga tgccgcctgg gttttgcgtt tcgatcggaa
      961 tttttccgaa atatccgcgc aagcatttat cgaccgcctg ctgcgtcaaa ccttgaatac
     1021 gcgctatttg ctcgtcggcg atgatttccg tttcggtgcg gggcgggaag gctgttttga
     1081 acttttggca caacagcccg atatgcaaac cgagcgcacg ccttccgtca tcgtcgaaga
     1141 catccgcacc agcagtaccg ccgtccgcca agccctttca gacggcaacc ttgcctatgc
     1201 gaagaaactt ttggggcacg actacgtttt ggggggcagg gtggtgcacg gcagaaaact
     1261 cggacgcacc ttaaacgccc cgaccgccaa tatccgcctg cccggccacc gttatgcact
     1321 cggcggcgtg ttcgtcgtcg aggcggacgg cgcattcggc acgcggcgcg gcgtggcgag
     1381 cttcggcttc aatcccaccg ttgatggcgg ctgttctcaa aagcttgaag tccacctgtt
     1441 cgactttcaa ggcgatttgt acggacaacg gttgaacgtc cgcttcctgc acaaactgcg
     1501 cgacgaggaa aagtttgacg gtatggaaga actgaaaagg cggattgaag ccgatatgga
     1561 agccgcaaag tgttggtaga aaaaccttat acaaaccatc cgattgggct acaatcagcc
     1621 ttttaactgt tcggacggca caggggtttc ccgttgtgaa atactgtttg aggcgcaatg
     1681 ccgtctgaaa ccgaaatatt gtaacaatag agattaaaaa atgaccgatt acagtaaaac
     1741 cgtcaacctg ctcgaaagcc cgtttccgat gcgcggcaat cttgccaagc gcgaacctgc
     1801 gtggctgaaa agctggtacg agcaaaaacg ttaccaaaaa ctgcgcgaaa tcgccaaagg
     1861 ccgtccgaaa ttcattctgc acgacggccc gccgtatgcc aacggcgaca tccatatcgg
     1921 tcatgccgtc aataaaattc ttaaagacat tattatccgc agcaaaaccc aagccggttt
     1981 tgacgcgcct tatgtaccgg gttgggactg ccacggtttg cctatcgaag taatggtgga
     2041 aaaactgcac ggcaaagata tgcctaaagc ccgtttccgc gaattgtgcc gcgaatatgc
     2101 cgccgaacag attgcccgtc agaaaaaaga ctttatccgc ttgggcgtgt tgggcgactg
     2161 ggacaatcct tacttgacca tggatttcaa aaccgaagcc gataccgtgc gtatgctcgg
     2221 cgaaatctac aaatccggct atctctaccg tggtgcgaaa ccggttcagt tttgcttgga
     2281 ttgcggatct tcgctggcgg aagcggaagt ggaatacaaa gacaaagtat cgcctgcgat
     2341 tgacgttgcc tatccgttta aaaacaccgt cgcgcttgcc gccgcattcg gcttggcagg
     2401 tatcgaaggc aaagcgtttg ccgtcatttg gacgaccacg ccttggactc tgcctgcgag
     2461 ccaggccgtg tctgccggcg cggacgtggt gtatcaatta atcgatacgc ccaaaggcaa
     2521 attggtgctg gcgaaagatt tggcggaagg cgctttgaaa cgctacggct tttcagacgg
     2581 catcgccatc cttgccgaaa ccaccggcga caagctggaa aacctgcaca tgaatcatcc
     2641 gttcctcgaa cgcgatattc ccatgctcaa cggcgaacac gttaccaccg atgccggtac
     2701 cggcttggtg catactgcgc ctgcgcacgg tttggaagac tacgccgtct gcaataaata
     2761 cggcatcgag ctttacaacc ctgtcaacgc cgaaggcaaa tacataagcg aaacgcctcg
     2821 tgtcgcaggc atgagcgttt gggaggcgaa tcccgtcatc ctgcaatggc cggaagaaac
     2881 cggcaacctc ttggcaagca gcaaaatcga acacagctac gcccactgct ggcgccacaa
     2941 aaccccgctg atttaccgag cgacaggtca gtggtttgtc ggcatggaca aagccggcag
     3001 cgacggtaaa accctgcgcg acaaagccat caaagccgtg gacgacaccg aattcttccc
     3061 gccatggggt cgtgcgcgtt tggaatccat gattgaaggc cgtcctgact gggtggtttc
     3121 acgccaacgc tattggggca cgccgatgac tttctttgtt cacaaagaaa cgggcgagct
     3181 gcatccgaac tctgccgaac ttttggaaaa agtcgcgcaa cgcatcgaag aaaaaggcat
     3241 cgaggcttgg ttctccctcg ataaaagcga attattaagc gcggaagatt gcgaacatta
     3301 cgacaaactc cccgatacca tggacgtatg gttcgactca ggctcgacgc attattccgt
     3361 tgtaaaacaa cgcgaagaat tggaatggcc ggctgacttg tacctcgaag gcagcgacca
     3421 acaccgcggc tggttccaat cctctatgct gaccggttgc gcctcatcca tgggacgcgc
     3481 accgtataaa cagctgctga cccacggttt cgtggttgac caaaacggcc gcaaaatgtc
     3541 gaaatccatc ggcaacgtcg tcgcgccgca ggaagtctat aacgaattcg gcgcggacat
     3601 cctgcgcctg tgggcggcat ccaccgatta cagcggcgaa ttggcgattt ccaaagaaat
     3661 cctcaaacgc gtaaccgaaa gctaccgccg catccgcaat accttgagct tcctgtttgc
     3721 caacttgagc gatttcaacc cgattgaaga tgccgtgcaa caggcggata tggtggaaat
     3781 cgaccgctac gccttggtat tggcgcggcg gctgcaagag cgtttggcag gcggttacta
     3841 tccgcgctat gccttccact tcgccgtgaa agacattgtt tctttctgct cggaagactt
     3901 gggcgcgttc tacctcgaca tcctgaaaga ccgcctctac accaccaaag ccgacagccg
     3961 cgcccgccgc agcgcgcaaa ccgccctgta ccacatcacg cgcagcctgg ttctcttgat
     4021 tgcaccgatt ttgtgcttca ccggcgaaga agcgtgggac atcatcggcg gcggcgaaga
     4081 agacagcgtc ctcttccata cttggcacga gttcccggcc atcaacgaaa aagccgaagc
     4141 cgaactggtg aaaaaatgga cggcaatccg cgaagcgcgc gaagcagtaa ccgccgccat
     4201 cgagcctttg cgcgccgaca aaaccgtcgg ttcgtccttg caggcggaag ccgaaattac
     4261 cgcgccggaa gaaatggccg gctatctgaa tgctttgggc gaagaattgc gctttgccct
     4321 gctggtgtct aaagcagaag tgaaagtcgg tgatgaactt gccgttgccg ccaaagccag
     4381 cgacggcgaa aaatgcgaac gctgctggca ttacacccgc gatgtgggcg cggttgcagg
     4441 ttatgaaacc gtctgcaaac gctgtgcgga gaatgtcggc ggagaaggcg aaacgcgcca
     4501 ttacgcctga taaagtttga gcaaatgccg tctgaaaccg ccaaacagta tttcagacgg
     4561 cattttttgt gccgcgattt gtcttcataa tggcggaggg gtttcgagat tgcggtgttg
     4621 tcgggaatga cggttcagtt gctacggtta ctgtcaggtt tcggttatgt tggaatttcg
     4681 ggaaacttat gaat
//
LOCUS       DAWTQS010000097         4582 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.097, whole genome shotgun sequence.
ACCESSION   DAWTQS010000097 DAWTQS010000000
VERSION     DAWTQS010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4582
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.097"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            <1..464
                     /locus_tag="WNA25_001972"
     CDS             <1..464
                     /locus_tag="WNA25_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HFA9824646.1"
                     /translation="VNILPFSKVKGLVKAAKLGRAAKPGRAAKLGKAKVSEDFAVASQ
                     ARYTSQTAENIIAADRIGSGLKADTSHRAASFLSQEQLGAGRTFSITGGDGIQRTLLQ
                     TKGSLNGQSGIYEYILDSNNKVTHQRFIKGGEITGQPNQRVKTKVRNRGKK"
     gene            461..934
                     /locus_tag="WNA25_001973"
     CDS             461..934
                     /locus_tag="WNA25_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2247 family protein"
                     /protein_id="HFA9824647.1"
                     /translation="MNLDLTAQKVRLSWKDILWGYENKYLGWADVAAYARKMTLSDHD
                     ERVFKLSLINKSNIFELKPVLEDLASEMRDYSPKNWLYILLNDVFHRKEEFEDPLGEV
                     EKIYADFDYPEEIESFVRYMPPKDGYIPSNHSYEENISRLYFNWGKYLSNKSRSG"
     gene            1063..1824
                     /locus_tag="WNA25_001974"
     CDS             1063..1824
                     /locus_tag="WNA25_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HFA9824648.1"
                     /translation="MVKTADGYKAIARIRAGESVLSKDEASGETGYKPVTARYGNPYQ
                     ETVYIEVSDGIGNSQTLISNRIHPFYSDGKWIKAEDLKAGIRLLSESGKTQTVRNIVV
                     KPKPLKAYNLTVADWHTYFVKGDKAETEGVWVHNDCPTKLKPTERYNRQTHYGGSQTD
                     GARAQAARQAGEGKPCPTCGRIQIFGTKTAPSPQHEPPLVKHYYEHGGHSMSNADRAK
                     HARESIKGTQCLTCQRKEGAMMSRYSREQAKKHGL"
     gene            1814..2362
                     /locus_tag="WNA25_001975"
     CDS             1814..2362
                     /locus_tag="WNA25_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HFA9824649.1"
                     /translation="MDYNQTVLSHLQKFWKHHDIKGFTWTLGRIVEELPDFQVFQVIP
                     NHEDEPWVYVSSGIGQFLGQEFFIISPFETPEHIETLAMLASASMHYPDQFQLGKTVN
                     IGRPWVEQSSFRHFLISLPYPYGQELEYMDNVRFFWLLPITQTERLFLNTHSVEELET
                     KFDEAGIDYLDINRASTVWQAG"
     gene            2659..3012
                     /locus_tag="WNA25_001976"
     CDS             2659..3012
                     /locus_tag="WNA25_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824650.1"
                     /translation="MGIKNEQIIICKKYNTEIYPVSDVSKIGVAENVKQTGLYPINGL
                     RHRPKGDTNGWYIWAGENFSYDKNFFLPLHTFHLQIWRPEIIPFLTLPPGYRFLIGEN
                     GYEDVWFDELLLNDN"
     gene            3002..3325
                     /locus_tag="WNA25_001977"
     CDS             3002..3325
                     /locus_tag="WNA25_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFA9824651.1"
                     /translation="MIIDEKSTSDVLKQYQELGFDLTKPMIIEFFIGGSQKNLQSIES
                     QLISYRQDFQISIEQDEFGEMWTCYCSVNIVPSLENILAIETTLFGIAQKNDCNYEGF
                     GSYGN"
     gene            3384..3611
                     /locus_tag="WNA25_001978"
     CDS             3384..3611
                     /locus_tag="WNA25_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824652.1"
                     /translation="MDIKFNTLGVILNGVNPEEKFIKIIDGQENTGGFLILLSSNDKF
                     SSFDSYDDWVENLEILKEYLQESHWIIKWVG"
     gene            3738..4281
                     /locus_tag="WNA25_001979"
                     /pseudo
     CDS             3738..4281
                     /locus_tag="WNA25_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227004.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
     gene            4383..4582
                     /locus_tag="WNA25_001980"
                     /pseudo
     CDS             4383..4582
                     /locus_tag="WNA25_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951255.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
BASE COUNT         1485 a          857 c          988 g         1252 t
ORIGIN      
        1 tggtcaacat cctgccgttt tccaaagtca aaggattggt gaaggcggcg aaactgggga
       61 gggcggcaaa accggggagg gctgcgaaac tagggaaggc aaaggttagc gaggattttg
      121 ctgttgccag tcaggcaaga tatacttcgc aaacggcaga aaatataatt gcagccgata
      181 gaataggaag tggattaaaa gctgatacgt cacatcgtgc agccagcttt ctatctcaag
      241 aacaattggg agcaggaaga acattctcaa ttactggcgg cgatggtatt caaagaactt
      301 tattgcagac aaaaggttct ttgaacggtc aaagcggtat atatgaatat atcctcgatt
      361 caaataacaa agtgactcat caaagattca ttaaaggagg tgaaattaca ggacaaccaa
      421 accaacgcgt gaaaactaaa gtacgcaacc gggggaaaaa atgaaccttg atttaaccgc
      481 tcaaaaagtc cgcctttctt ggaaggatat tctgtggggg tatgagaata aatacttggg
      541 ttgggctgat gtggcagctt atgcccgaaa aatgacgctt tcagatcatg atgaacgcgt
      601 gttcaagcta tctttaatca acaaatccaa tatttttgaa ttaaagcctg ttctagaaga
      661 tttggcttcg gaaatgaggg attattcccc taaaaattgg ctgtacatcc tcctaaatga
      721 cgtattccat agaaaagaag aatttgagga tcctctgggg gaagttgaaa aaatttatgc
      781 ggattttgat tacccggaag aaatagaatc atttgtcagg tatatgccgc ccaaagacgg
      841 ctatatccct tctaatcata gttatgaaga aaatatttct cggttatatt ttaattgggg
      901 aaaatatttg agcaacaaga gtaggtcggg ttaaaaattc aacaatcaaa aggtcgtctg
      961 aaactgcttt taattttcag gcgaccttcc ccttatttta aacaggatat tgggaactaa
     1021 gttcttcaaa aatcctacac ctgctccttc cacggcagca ccttggtcaa aacggcagac
     1081 ggctacaaag ccattgcccg tatccgagcc ggcgagagcg tcctctccaa ggacgaggca
     1141 agcggagaaa cgggatacaa acccgttacc gcccgatacg gtaatccgta ccaagaaacc
     1201 gtttacattg aagtttcaga cggcatcggc aacagccaaa ccctgatttc caaccgcatc
     1261 catccgtttt attcggacgg caaatggatt aaagcggaag atttaaaggc gggaatcagg
     1321 ctgttatccg aaagcggcaa aacccaaacc gtccgcaaca tcgttgtcaa accaaaaccg
     1381 ctcaaagcct acaatctgac cgttgccgat tggcatacct actttgtcaa aggcgataag
     1441 gcggaaacgg aaggggtttg ggttcataat gattgtccaa ccaaactaaa accgacagaa
     1501 cgttataatc gtcaaacgca ttatggaggt tctcaaacag atggagcaag ggctcaggca
     1561 gcaagacagg caggagaagg taaaccttgt ccaacttgtg gtagaattca gatatttggt
     1621 acgaaaaccg caccaagtcc gcaacatgaa cccccattag taaaacatta ctatgaacat
     1681 ggtggtcact ctatgagcaa tgctgatcga gcgaaacatg ccagggaaag cataaaagga
     1741 actcaatgtt taacatgtca aaggaaagag ggtgcgatga tgtcaagata ttcgagagaa
     1801 caggctaaaa agcatggact ataaccaaac tgttttatct cacttgcaaa aattttggaa
     1861 acatcatgat attaaaggat ttacttggac tttaggaaga attgttgaag aacttcccga
     1921 tttccaagtt ttccaagtga ttcctaatca tgaagatgag ccttgggttt atgtcagctc
     1981 tgggataggt caatttttag ggcaggagtt ttttatcatc agcccgtttg aaacacctga
     2041 acatattgag accttggcaa tgctggcatc tgcaagtatg cattatcctg atcaatttca
     2101 attagggaaa accgtcaata tcggtagacc gtgggtcgag caatcatcct tccggcattt
     2161 tctgatttct ctgccttatc cttatgggca agagttggaa tatatggata atgtccgatt
     2221 tttctggtta ttgccaatta cccagacaga aagacttttc ttgaatactc attcggtaga
     2281 agaattggaa acgaaatttg atgaagcggg tattgattat ttagatataa atcgtgcgag
     2341 tactgtctgg caggcaggat aaaaattcgg caatcaaaat gccgtctgaa acaagtttgg
     2401 actttcagac ggtattctga tgaatggaac aggatactgg gaactgagtt cttcaaaaat
     2461 cctatctccg tcccttatac gtcggtgctt tagtcaaaac ggcagacgga tgaaaaatta
     2521 ttgcccgtat ccttgccaaa gatgaagcaa gatgaagcca ggtaattgaa tcggtacaag
     2581 cgcaatgtcc gacttgttct gcaaaacagg gagggagatt aagccaatat tcaaaagaac
     2641 agaaaaagaa actgagtaat gggaataaaa aatgaacaaa taataatttg taaaaaatat
     2701 aatacagaaa tatatcctgt ttccgatgtt tcaaaaatcg gtgttgctga aaatgtcaaa
     2761 cagacaggcc tgtatccgat taacgggtta aggcaccgtc ccaaaggtga tactaacggt
     2821 tggtatatct gggccggtga aaacttctcc tatgataaaa attttttcct gcccttgcat
     2881 acgttccatt tgcaaatatg gcgacccgaa atcatcccct ttttgacact tccgcccggt
     2941 taccgttttt taattgggga aaacggttat gaagacgtat ggtttgatga attgctatta
     3001 aatgataatt gacgaaaaat ctacttcaga tgtactgaaa caatatcaag aactagggtt
     3061 tgatttgaca aaacctatga taatagagtt ttttatagga ggttctcaaa aaaatctgca
     3121 atcaatagaa agccaactca tttcttatcg gcaagatttt caaatcagta ttgaacaaga
     3181 tgaattcgga gaaatgtgga cttgctattg ttccgtcaat atcgtccctt cccttgaaaa
     3241 cattttagct attgaaacta cgttatttgg tattgcacaa aaaaatgatt gtaattatga
     3301 aggttttggt tcgtatggga actaacaaaa agtgtaggtt gggttgaaaa aaccatattc
     3361 ccattaaaga ttagggtgtg tctatggaca taaaattcaa tactttgggg gttatcctga
     3421 atggggtcaa tcctgaagaa aaattcataa aaattattga tggccaggaa aataccggtg
     3481 gatttctgat actgttatct tcaaatgaca aattctcatc atttgacagt tacgatgact
     3541 gggtcgaaaa cttagaaata ttaaaagaat acctgcaaga atctcattgg ataataaagt
     3601 gggtcggata gtcggcaaac gtagtccgtt tgaaacgctt ttacttttca gacggacttt
     3661 ctaccaatgg gaacaaggcg ttttgtctca atccggttta tgattattcg atacagtgat
     3721 gggaagctaa gaaaaatatg attaatcata ttgaaaaata tataggcact atttctcatg
     3781 gctcgaaaag cgattcggga agtcagtaca aattaaatat cgcagctatt ccctcttccc
     3841 ctaacaggga tttaaaaact tatattactt tagggttaag caaacatgat ctgaattata
     3901 aaagcagatt tgaaatactt tttgtatgtt ctttaaaata tgatgaaaat cagattttcc
     3961 catttttaag atggttagca gaaaccatca ttgagaataa aaaaaatcct tcttcggggg
     4021 caggttgttt atttgcctag aagcattgtt aattcaacaa aaatggacgc actgtatgtt
     4081 tccgccccat tttatttcga cgatgatttc caagtctgtt atggcgaaca ctacaatatt
     4141 gttttccctt tgcttgtccc cttgtataaa caggaagccg aattggtgga aaaaaagggt
     4201 tggaatgctt ttgagcagtt cttgttggat aatgaagttg gcaacctttc ggatatgaat
     4261 aggaagccgt ttgtttggta agggctttct taaatttgat taaaggtcgt ctgaaaccgt
     4321 gtttaatttt cagacggcat ttccttcatt tgaaacagga tattgaaaac taagttcttc
     4381 aaaaatccta cacctgctcc ttccacggca gcaccttggt caaaacggca gacggctaca
     4441 aagccatcgc ccatattcaa gccggagacc gcgtcctttc caaggacgag gcaagcggag
     4501 aaacgggata caaacccgtt accgcccgat acggtaatcc gtatcgggaa accgtttaca
     4561 ttaaagtttc agacggcatc gg
//
LOCUS       DAWTQS010000098         4386 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.098, whole genome shotgun sequence.
ACCESSION   DAWTQS010000098 DAWTQS010000000
VERSION     DAWTQS010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4386
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.098"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(<1..1212)
                     /locus_tag="WNA25_001981"
     CDS             complement(<1..1212)
                     /locus_tag="WNA25_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824653.1"
                     /translation="MKQKKTVQCILLGFAAASMHAQGAAAANSGTIEKTDKYTLVLAK
                     QGQENNYTLNGGTEVKPLNSLIIAANGGTNNITIKGKLADGPADAPPTIDNNSIERNI
                     NKNGYTYAWQNWSGAVMLVDQSYEGENKVTFENVTIAAHNAPAGILSDDRHKSSSLAP
                     AMLAFKGRNTINMDADSNANSSNEGILLLNNGEKMGEYRLVSEEGSTLNINIKSGKDK
                     GQGITANHYGNSDINFNKASPNITTMEFKGDVNIKIDRNGQEEAESNGFGFYSSRKLG
                     NKKQIPEGSKMEAIFRGNVDIVATPVYDEQGRPKSIGSAFAIDGKYSKVEVVGGEGKV
                     VKIKGDIFAYNGGSVSVNLANKDSYFEGEAHIGKRSFAKGKDMFALTVDADGYELTPD
                     TKSIEKKKKELN"
     gene            1409..1484
                     /locus_tag="WNA25_001982"
     tRNA            1409..1484
                     /locus_tag="WNA25_001982"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1443..1445,aa:Glu,seq:ttc)
     gene            complement(1700..1852)
                     /locus_tag="WNA25_001983"
     CDS             complement(1700..1852)
                     /locus_tag="WNA25_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824654.1"
                     /translation="MPSENRSAGFVVHYYVVLQDKAQGKAAGCIVATDNEKRGRCRLN
                     RVLRRV"
     gene            1912..2718
                     /gene="thiD"
                     /locus_tag="WNA25_001984"
     CDS             1912..2718
                     /gene="thiD"
                     /locus_tag="WNA25_001984"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFA9824655.1"
                     /translation="MKGSFVQTLAIAGSDSGGGAGIQADLKTFQMRGVFGTCVITAVT
                     AQNTLGVSAVHLVPTETITAQIQAIREDFDIRAYKIGMLGTAEIIECVADKLKHCSFG
                     RRVLDPVMIAKGGAPLLQDSAVAALTRLLLPDTDILTPNLPEAEALTGVHIENRKDAE
                     RAAKILLDYGVKNVIIKGGHLNGSTSGRCTDWLFTQNETLELDSPRFPTAHTHGTGCT
                     FSACITAELAKGLDVCKAVQTAKAYITAAISNPLEIGAGHGPVNHWAYRD"
     gene            complement(2776..3645)
                     /gene="tehB"
                     /locus_tag="WNA25_001985"
     CDS             complement(2776..3645)
                     /gene="tehB"
                     /locus_tag="WNA25_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HFA9824656.1"
                     /translation="MKERIVGQSGELFCFGQMPVWKVENLPEVLLSGYSSEEGEWVCL
                     NVLQGDVEVRAPDGAAEVWSAESGDCVFAPQQVFSVKPKTDDAEIRLSLYCAAADYFH
                     KKYGMSATHSAVAAAQDTVPAGRALDMGCGQGRNALFLGLKGFDVTAADCNPAALANV
                     AELAEAEGLNVRTLEYDLNAAALQGEFDYIVATVVMMFLMPQRVPDVIADMQAHTAAG
                     GYNLIVSAMDTADFPCPMPFPFKFKEGELKDYYRDWELVEYKEELGAMHAKDENGNPI
                     RFKFVTMLAKKPG"
     gene            3758..4195
                     /gene="rnhA"
                     /locus_tag="WNA25_001986"
     CDS             3758..4195
                     /gene="rnhA"
                     /locus_tag="WNA25_001986"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFA9824657.1"
                     /translation="MDTPVYLYTDGACKGNPGAGGWGVLMRYGSREKELFGGEAQTTN
                     NRMELTAVIEGLKSLKRRCTVIICTDSQYVKNGMENWIHGWKRNGWKTAAKQPVKNDD
                     LWQELDALVGQHQVSWTWVKGHAGHAENERADDLANRGAAQFS"
BASE COUNT         1010 a         1234 c         1037 g         1105 t
ORIGIN      
        1 attcagctct ttctttttct tttcaatcga ctttgtatcg ggggttaatt catatccgtc
       61 cgcatccacg gttaaggcaa acatatcttt ccctttggca aagctccttt tcccgatatg
      121 ggcttcccct tcaaaataag agtctttgtt ggcaaggttc acgctcacgc tgccgccgtt
      181 gtaggcgaaa atatcgcctt tgattttgac aaccttgcct tccccgccga caacctcgac
      241 tttgctgtat ttcccgtcaa tcgcaaacgc gctgcctatg cttttgggcc tgccttgctc
      301 gtcataaacc ggcgttgcaa cgatatcgac gttcccacgg aaaatcgctt ccatcttaga
      361 accttccgga atctgcttct tattgcctaa tttgcggctt gaatagaagc caaaaccgtt
      421 gctttccgcc tcttcctgtc cgttcctatc gattttaatg tttacatcgc ccttgaactc
      481 cattgtggtg atgtttggag aagccttgtt gaagttgatg tctgagttac catagtggtt
      541 ggcggtaatg ccctgtcctt tatcttttcc tgatttgatg ttgatattca gcgtcgagcc
      601 ttcttcggaa acaagacgat attctcccat tttttcacca ttattaagca ataggatacc
      661 ttcgttacta gagttggcat ttgagtccgc gtccatattg atggtattgc gccctttaaa
      721 ggcaagcatc gccggcgcca agctactgga tttatgccta tcatcagaca aaatgccggc
      781 aggcgcgtta tgggcggcta tggttacgtt ttcaaaagta accttatttt ctccctcata
      841 gctttgatcc accaacatca ccgcgccgct ccaattctgc catgcgtacg tgtaaccgtt
      901 tttatttata tttctttcaa tcgagttatt gtcaatcgtc ggcggggcat cggcaggacc
      961 gtctgccaat ttgcctttta tcgtaatgtt gttcgtaccg ccgttggcgg caatgatgag
     1021 ggaatttaag ggtttgactt cggttccgcc attaagggta tagttgttct cttgcccttg
     1081 ttttgcgagg acgagggtgt atttgtctgt cttctctatc gttccactgt ttgcagcggc
     1141 cgccccttgc gcgtgcatag aagcggccgc gaagccaagt aagatgcact gaactgtctt
     1201 cttttgtttc atcggttttt ccattttgaa ggtatgtgat ttttatcatt atatgcttaa
     1261 atcattgaca cgcccccaaa aaaaatgaat tgcttgatat tccaatgttt tttatcttgt
     1321 gatacggtca gacaaacgcc ccctgacatt cgtttagacg gcatcgtatt gctaaatttc
     1381 tataagtatg tataatgtcc gtttccacgc gcccatcgtc tagaggccta ggacactgcc
     1441 ctttcacggc ggcaaccggg gttcgaatcc ccgtgggcgt gccaattcaa aaacctgctt
     1501 gtttcaagca ggttttttat tatgaatcgt tattcccgca atttttcgtc attgccgtga
     1561 aaacgggaat ctagaacctc aaactttcag ataatctttg aatattgctg ttgttctaag
     1621 gtccggattc ccgcctgcgc gggaatgacg gaattttagg tttctgtttt tgcgggaatg
     1681 acgaatgaag cgtgccggtt tatactcgcc gcaacacgcg attcagacgg catcgccctc
     1741 ttttttcatt atcagttgct acgatgcacc ccgctgcctt gccctgtgcc ttgtcctgca
     1801 atacgacata ataatgcacc acaaaccccg cgctgcggtt ttcagacggc attgcctgct
     1861 tttttacagg cagtagccct ttttatcgga cgcaatatta aggaggaaca aatgaaaggc
     1921 tcttttgtgc agacgcttgc catcgccggt tcggattcgg gcggcggtgc gggcattcag
     1981 gcggatttga aaacgtttca gatgcgcggc gtgttcggaa cgtgtgtcat caccgccgtt
     2041 accgcgcaaa ataccttggg cgtgtcggcg gttcatctcg tcccgaccga aaccatcacc
     2101 gcacaaatcc aagcaatcag ggaagacttt gacatccgcg cctacaaaat cggtatgctc
     2161 ggcacggcgg aaatcatcga atgcgttgcc gacaagctga aacactgcag ctttggcagg
     2221 cgcgtactcg accctgtgat gattgccaaa ggcggtgcgc cgctgttgca ggattccgcc
     2281 gttgcggcac tgacgcgcct gctgctgccg gacacggaca tcctgacccc caacctgccc
     2341 gaggcggaag ccttgacggg cgtgcatatc gaaaaccgta aagatgcgga acgtgcggca
     2401 aaaatcctgc ttgattacgg tgtcaaaaat gtcattatca aaggcggaca tttgaacggc
     2461 agcacaagcg gacgctgcac ggattggctg tttacgcaaa acgaaacgct ggaattggac
     2521 agcccgcgct ttccgaccgc ccacacgcac ggcacgggct gcacgttttc cgcctgcatt
     2581 accgccgagt tggcaaaagg cttagacgtt tgcaaagccg tacagactgc caaggcctac
     2641 atcacggcgg caatctcaaa ccctttggaa atcggcgcgg gacacgggcc ggtcaatcat
     2701 tgggcgtatc gggactagac gtaaaaatgc cgtctgaacg ccttttcggg ttcagacggc
     2761 atcgtccggc gcggcttatc ctggtttttt cgccagcata gttacaaatt taaaccgtat
     2821 cgggttgccg ttttcgtctt tggcgtgcat cgcgcccaat tcttctttat attcgaccag
     2881 ctcccaatcc cgataataat ccttcagctc gccctcttta aatttgaagg ggaacggcat
     2941 cgggcagggg aaatccgccg tatccattgc cgatacgatt aagttgtacc cgcccgccgc
     3001 cgtatgcgcc tgcatatcgg caatcacgtc gggcacgcgc tgcggcatca ggaacatcat
     3061 caccaccgtt gccacaatat aatcaaactc gccctgaagg gcggcggcgt tcaaatcata
     3121 ttccagcgtg cggacgttca aaccctccgc ctctgccagt tccgccacgt ttgccaacgc
     3181 ggcgggattg caatcggcag cggttacatc aaaccccttc aaacccagga acagcgcgtt
     3241 gcgcccctgt ccgcagccca tatccaacgc cctgcccgcc ggtacggtat cctgtgccgc
     3301 cgcgaccgca gaatgcgtgg cactcatccc gtattttttg tgaaaatagt ctgccgccgc
     3361 gcaatacagc gacaaacgga tttcggcatc gtccgttttc ggtttgaccg aaaacacctg
     3421 ctgcggcgca aacacgcaat cgccgctttc cgccgaccaa acctctgccg ccccgtccgg
     3481 tgcacggact tcgacatcgc cctgcaacac attcaggcag acccattccc cttcctcgga
     3541 cgaataaccc gacaacaaaa cttccggcag gttttccact ttccatacag gcatttgtcc
     3601 gaaacaaaac aactcgccac tttgacccac tatccgctcc ttcatattca aaaataaagt
     3661 tgcacattat atgcctattt caatccgccg caatctttca gacggcacga cgcacaaacc
     3721 gcttataatc acgccggaca ccacacaaag gcacgatatg gacacacccg tttacctcta
     3781 cacggacggc gcgtgcaaag gcaatcccgg cgcgggcggc tggggcgtat taatgcgcta
     3841 cggcagccgc gaaaaagaac ttttcggcgg cgaagcgcaa accaccaaca accgcatgga
     3901 gctgaccgcc gttatcgaag ggctgaaatc gctcaaacgc cgctgcaccg tcatcatctg
     3961 caccgactcg caatacgtca aaaacggcat ggaaaactgg atacacggtt ggaagcgcaa
     4021 cggctggaaa accgccgcca aacagcccgt caaaaacgac gacttgtggc aagaactcga
     4081 cgctctggtc gggcagcatc aagtcagctg gacttgggtt aaaggacacg cgggacacgc
     4141 ggaaaacgaa cgcgccgacg atttggcaaa ccgtggcgcg gcgcagtttt cttgattgcc
     4201 gcaccgacaa aaatgccgtc tgaaaccgct aatgggcttc agacggcatc gtcctccacc
     4261 gtcattcccg cgcaggcggg aatccagaac gtcgggcaac ggcaatattc aaaagccgtc
     4321 tgaaaattta aaagttctag attcccgttt tcacgggaat gacgaaaagt tgcggacagc
     4381 agagga
//
LOCUS       DAWTQS010000099         4247 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.099, whole genome shotgun sequence.
ACCESSION   DAWTQS010000099 DAWTQS010000000
VERSION     DAWTQS010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4247
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.099"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            <1..285
                     /locus_tag="WNA25_001987"
     CDS             <1..285
                     /locus_tag="WNA25_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hint domain-/BECR-fold containing toxin"
                     /protein_id="HFA9824658.1"
                     /translation="VWVHNACPPRKTPSTPVYGNDSEAYAAAKKLGYRKIKERTRNDA
                     AIFKKGKSYISRDVDSHNGGAWKEASSPKNLNRKETRNGTFDKNLNRIGD"
     gene            291..689
                     /locus_tag="WNA25_001988"
     CDS             291..689
                     /locus_tag="WNA25_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HFA9824659.1"
                     /translation="MCEFKDFRRNIPCFEEYDENSFIGKWYDDGVWDDEEYWKLENAL
                     IEVRKKYPYPMDIPRDIVIGIGTIIDFLMVQNWKLFEIKASPWLPKSVKINERYERFR
                     VMLRYIFTDLDAEDWKFFYFPIQHSKGRLR"
     gene            720..1097
                     /locus_tag="WNA25_001989"
     CDS             720..1097
                     /locus_tag="WNA25_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HFA9824660.1"
                     /translation="MCEFKDIIRNVPYFEGYDENSFIGKWYDDGVWDDEEYWKLENDL
                     IEVRRKYPYPMDIPRDIVIGIGTIIEFLMVPNWKLFEIKASPWLPDSVGINERYERLK
                     TMLRYIFTEKDIVNVQFDYYNKK"
     gene            1228..1965
                     /locus_tag="WNA25_001990"
     CDS             1228..1965
                     /locus_tag="WNA25_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFA9824661.1"
                     /translation="MVRTADGYKAIAHIQAGDRVLSKDEASGKTGYKPVTARYGNPYQ
                     ETVYIKVSDGIGNSQTLISNRIHPFYSDGKWIKAEDLKAGNRLFAESGKTQTVRNIVV
                     KPKPLKAYNLTVADWHTYFVKGDKAETEGVWVHNDCPYGGSNNLEKAKLRAERLSKND
                     RAGKDFTKAGKEAVIDLNRIQNNGQVKCANCGIETIPAKQSIKNISPTSNERQVDHVI
                     PKSKGGQGTPKNGQVLCRGCNIKKSNK"
     gene            1962..2381
                     /locus_tag="WNA25_001991"
     CDS             1962..2381
                     /locus_tag="WNA25_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4265 domain-containing protein"
                     /protein_id="HFA9824662.1"
                     /translation="MKSKLTVVYYDLESNIAEEILSGNIMPDGNFLIQEIPLFAPNLA
                     LNDIVAIEREDKMLFFDHLIKASGNTTINIVVLDHFPKDLLAAIEEHSGKIRKNGENY
                     LSVNFPPKKYNSDLKGILNRYEEANILSYREACLGFS"
     gene            2548..3279
                     /locus_tag="WNA25_001992"
     CDS             2548..3279
                     /locus_tag="WNA25_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HFA9824663.1"
                     /translation="MVKTADGYKAIARIRAGESVLSKDEASGVTGCKPVTARYGNPYR
                     ETVYIEVSDGIGNSQTLISNRIHSFYSGGKWIKAEDLKAGSRLLSESGRTQTVRKTVV
                     KPKPLKAYNLTVADWHTYFVKGSQAETEGVWVHNDCPYGNLSDNKSVGEGKKFTPAQK
                     KAIIQENMNRNGGVVKSDQSGEVLVRPKKSQKGITPPHQMNGRLTMCKQKVKEVQIVI
                     KMHRFYPEEKILRNQINRYQYAMEI"
     gene            3263..3535
                     /locus_tag="WNA25_001993"
     CDS             3263..3535
                     /locus_tag="WNA25_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824664.1"
                     /translation="MQWKFEDPQNTAVITTRRILSKKNGILEVWHDADDGMWQFLDGN
                     EIHEGDAVIVSLKEIVTIDSSVNLLSNLPLGGYAWRNTISEKWNFD"
     gene            3702..3932
                     /locus_tag="WNA25_001994"
     CDS             3702..3932
                     /locus_tag="WNA25_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HFA9824665.1"
                     /translation="METLDERIKNLGKSLEDRIDANLIDATLEYITFSERLLAFETLC
                     DYIEDFNIQLTEKESQEISFINKEFGIESTSD"
     gene            4081..>4247
                     /locus_tag="WNA25_001995"
     CDS             4081..>4247
                     /locus_tag="WNA25_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pretoxin"
                     /protein_id="HFA9824666.1"
                     /translation="MVKTADGYKAIARIRAGESVLSKDEASGVTGCKPVTARYGNPYR
                     ETVYIEVSDGIG"
BASE COUNT         1455 a          775 c          898 g         1119 t
ORIGIN      
        1 gtttgggttc ataatgcgtg tccgccaaga aaaacaccat ctacccccgt atatgggaat
       61 gattcagaag catatgcagc agcaaagaaa ttgggatata gaaaaataaa agaaagaacg
      121 agaaatgatg ctgctatttt taaaaaagga aagagttata tatcacgcga tgtagattca
      181 cataatggag gggcttggaa agaagcatct tcacccaaaa atctgaacag aaaagaaaca
      241 agaaacggta catttgataa aaacctaaat cgtattggag attaattatt atgtgcgagt
      301 tcaaggattt tagaagaaac atcccttgtt ttgaagagta tgacgaaaat tcatttattg
      361 gcaaatggta tgatgacgga gtgtgggatg atgaagaata ttggaaattg gagaatgctt
      421 taatcgaggt tagaaaaaaa tatccttatc cgatggatat accaagggac atcgtgattg
      481 gaatcggtac cattattgat tttttaatgg ttcaaaattg gaaacttttt gaaattaaag
      541 cttctccttg gttgcctaaa agtgtcaaaa ttaatgagcg ttatgaaagg ttcagagtaa
      601 tgctccgtta tatttttact gatctagatg cagaggactg gaaatttttt tactttccaa
      661 tacaacatag taaaggtaga ttgagatgaa aagtttaatt atattggaga tacattatta
      721 tgtgtgagtt caaggatatt ataagaaacg ttccttattt tgaggggtat gacgaaaatt
      781 catttattgg caaatggtat gatgacgggg tgtgggatga tgaagaatat tggaagttgg
      841 agaatgattt aatcgaggtt aggagaaaat atccttatcc gatggatata ccaagggata
      901 tcgtgattgg aatcggtacc attattgagt ttttaatggt tccaaattgg aaactttttg
      961 aaattaaagc ttccccttgg ttacctgata gcgtgggaat taacgaacgt tatgaaaggc
     1021 ttaaaacaat gctccgttat atttttaccg agaaagacat agtcaacgtg caatttgatt
     1081 attacaacaa aaaatagcaa aacccggcaa tcaaaatgcc gtctgaaacc gtgtttaatt
     1141 ttcaggcgac cttcccctta ttttaaacag gatattggga actaagttct tcaaaaatcc
     1201 tacacctgct ctttccacgg cagcaccttg gtcagaacgg cagacggcta caaagccatc
     1261 gcccatattc aagccggaga ccgcgtcctt tccaaggacg aggcaagcgg gaaaacagga
     1321 tacaaacccg ttaccgcccg atacggcaat ccgtatcaag aaaccgttta cattaaagtt
     1381 tcagacggca tcggcaacag ccaaaccctg atttccaacc gcatccaccc gttctattcg
     1441 gacggcaaat ggattaaggc ggaagattta aaagcgggaa acaggctgtt tgccgaaagc
     1501 ggcaaaaccc aaaccgtccg caacatcgtt gtcaaaccaa aaccgctcaa agcctacaat
     1561 ctgaccgttg ccgactggca tacctacttt gtcaaaggcg ataaggcgga aacggaaggg
     1621 gtttgggttc ataatgattg tccgtatggc ggttcaaata atttagagaa agcaaaactt
     1681 cgcgcagaac ggcttagtaa aaacgatcgg gcaggaaaag actttactaa agcaggaaaa
     1741 gaagccgtta ttgatttgaa taggattcaa aataatggtc aagttaagtg cgctaattgc
     1801 ggtattgaaa caattccagc caaacaatct ataaaaaata tttctcctac ttcaaatgaa
     1861 agacaagtag atcatgtcat ccctaaatct aagggtggtc aaggtacacc taaaaacggg
     1921 caggtattat gcagaggctg caacattaag aagagtaaca aatgaaatcg aaacttactg
     1981 tcgtctatta cgatttggaa agcaatattg cagaagaaat actgtcagga aacataatgc
     2041 ctgatggaaa ttttcttatt caagagattc ctcttttcgc accgaatttg gctttaaacg
     2101 acattgttgc catagaacgt gaggataaga tgctgttttt cgaccacttg ataaaagctt
     2161 caggaaatac cacgataaac atcgttgttt tggatcattt cccaaaggat ttattggcag
     2221 ccatagaaga acacagtggt aaaatcagaa aaaatggaga gaattattta tcggttaatt
     2281 ttccgcccaa aaaatataat tctgatttaa aaggaatttt aaatagatat gaggaagcaa
     2341 atatcctcag ctacagggaa gcttgtttgg gcttctctta atcgatctat aagtaagaat
     2401 ggattacgtt agattgtaaa cctaacgatt aaaaggtcgt ctgaaaccgt gtttaatttt
     2461 cagacgacct atttccttca tttgaaacag gatattggga attaagtttt tcaaaaatcc
     2521 tacacctgct ccttccacgg cagcaccttg gtcaaaacgg cagacggcta caaagccatt
     2581 gcccgtatcc gagccggcga gagcgtcctc tccaaggacg aggcaagcgg ggtaacggga
     2641 tgcaaacctg ttaccgcccg atacggcaat ccgtatcggg aaaccgttta cattgaagtt
     2701 tcagacggca tcggcaacag ccaaaccctg atttccaacc gcatccactc gttttattcg
     2761 ggcggcaaat ggattaaggc ggaagattta aaagcgggaa gcagactgtt atccgaaagc
     2821 ggcagaaccc aaaccgtccg caaaaccgtt gtcaaaccaa aaccgctcaa agcctacaat
     2881 ctgaccgttg ccgactggca tacctacttc gtcaagggca gtcaggcgga aacggaaggg
     2941 gtttgggttc ataatgattg tccgtatgga aatttatcgg ataataaatc tgttggcgaa
     3001 ggaaaaaaat ttacaccagc tcaaaagaaa gcaatcatcc aagaaaacat gaatagaaac
     3061 ggaggggtag tgaagtctga ccaatctgga gaagttttgg ttcgtcctaa aaagagccaa
     3121 aaaggtatca ccccccccca tcaaatgaat ggcagattga ccatgtgcaa gcaaaaagta
     3181 aaggaggttc aaatagttat aaaaatgcac aggttttatc cagaagagaa aatattaaga
     3241 aatcaaataa ataggtatca atatgcaatg gaaatttgaa gatcctcaaa atactgctgt
     3301 tattaccacc agaagaattt tgtctaaaaa aaacggaatc ttagaagtat ggcatgatgc
     3361 tgatgatgga atgtggcaat tcttagatgg caatgaaatt catgaaggag atgctgtgat
     3421 agttagttta aaagaaattg tcactattga ttcctcagtc aatctcttaa gtaatttacc
     3481 tcttggtggt tacgcttgga gaaatactat atcggaaaaa tggaattttg attagcaagc
     3541 gtaggttagg ttaaaaaaac caacaatcaa aggtcgtctg aaaccgtgtt tagtttttca
     3601 aacgaccttt tcttcaatta acatagaatg ttgggtctcg acccaaccta cacgcttact
     3661 aatctcaaat ataaccttat gaaataatga gtatttaaaa gatggaaaca ttagacgaac
     3721 gaataaagaa tttaggaaaa tctcttgaag atagaattga cgctaatcta attgatgcca
     3781 cactagagta cattactttt tcagaacgtt tgctagcttt tgaaacgcta tgtgattata
     3841 ttgaagattt caatatccag cttacggaaa aagaatccca agaaatctct tttattaata
     3901 aggaatttgg aattgaaagt acatcagatt aataaatatt aggtcggttt aaaaacccaa
     3961 caatcacaag gtcttctgaa accgtgttta attttcagac ggcatttcct tcatttgaaa
     4021 caggatattg ggaattaagt ttttcaaaaa tcctacacct gctccttcca cggcagcacc
     4081 ttggtcaaaa cggcagacgg ctacaaagcc attgcccgta tccgagccgg cgagagcgtc
     4141 ctctccaagg acgaggcaag cggggtaacg ggatgcaaac ctgttaccgc ccgatacggc
     4201 aatccgtatc gggaaaccgt ttacattgaa gtttcagacg gcatcgg
//
LOCUS       DAWTQS010000100         4215 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.100, whole genome shotgun sequence.
ACCESSION   DAWTQS010000100 DAWTQS010000000
VERSION     DAWTQS010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4215
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.100"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(119..880)
                     /locus_tag="WNA25_001996"
                     /pseudo
     CDS             complement(119..880)
                     /locus_tag="WNA25_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
     gene            1326..2258
                     /gene="pip"
                     /locus_tag="WNA25_001997"
     CDS             1326..2258
                     /gene="pip"
                     /locus_tag="WNA25_001997"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HFA9824667.1"
                     /translation="MYEIKQPFHSGYLQVSEIHQIYWEESGNPDGVPVIFLHGGPGAG
                     ASPECRGFFNPDVFRIVIIDQRGCGRSHPYACAEDNTTWDLVADIEKVREMLGIGKWL
                     VFGGSWGSTLSLAYAQTHPERVKGLVLRGIFLCRPSETAWLNEAGGVSRIYPEQWQKF
                     VAPIAENRRNRLIEAYHGLLFHQDEEVCLSAAKAWADWESYLIRFEPEGVDEDAYASL
                     AIARLENHYFVNGGWLQGDKAILNNIGKIRHIPTVIVQGRYDLCTPMQSAWELSKAFP
                     EAELRVVQAGHCAFDPPLADALVQAVEDILPRLL"
     gene            complement(2315..3511)
                     /gene="bamC"
                     /locus_tag="WNA25_001998"
     CDS             complement(2315..3511)
                     /gene="bamC"
                     /locus_tag="WNA25_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFA9824668.1"
                     /translation="MPSEPFGRHNMTNTLISITQDDTMTHIKPVIAALALIGLAACSG
                     SKTEQPKLDYQSRSHRLIKLEVPPDLNNPDQGNLYRLPAGSGAVRASDLEKRRTPAVQ
                     QPADAEVLKSVKGVRLERDGSQRWLVVDGKSPAEIWPLLKAFWQENGFDIESEEPAIG
                     QMETEWAENRAKIPQDSLRRLFDTVGLGGIYSTGERDKFIVRIEQGKNGVSDIFFAHK
                     AMKEVYGDKNKDTTMWQPSASDPNLEAAFLTRFMQYLGVDGRQAENALAKKPTLPAAN
                     EMARIEGKSLIVFGDYGRNWRRTGLALDRIGLTVVGQNTERHAFLVQKAPNESNAVTE
                     QKPGLFKRLLGKGKAEKPAEQPELIVYAEPVADGSRIVLLNKDGSAYAGKDASALLGK
                     LHSELR"
     gene            complement(3520..>4215)
                     /locus_tag="WNA25_001999"
     CDS             complement(3520..>4215)
                     /locus_tag="WNA25_001999"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFA9824669.1"
                     /translation="EETVKHVAKRVPVIAGTGANNTVEAIALSQAAEKAGADYTLSVV
                     PYYNKPSQEGMYRHFKAVAEAAAVPMILYNVPGRTVVSMNNETILRLAEIPNIVGVKE
                     ASGNVGSNIELINRAPEGFVVLSGDDHTALPFMLCGGHGVITVAANAAPKLFADMCRA
                     ALQGNIALARELNDRLIPIYDTMFCEPSPAAPKWAVSALGRCGPHVRLPLVPLTEGGQ
                     AKVRAALKASGQL"
BASE COUNT          792 a          944 c         1354 g         1125 t
ORIGIN      
        1 attgcggtgt tgtcgggaat gacggttcgg gtattttact gcgcccgccc cgcgcctgta
       61 aacggcgggc gcatcaaaaa tgccgtctga aggttcagac ggcatcggta tcggggaatc
      121 agaagcggta gcgcatgccc aatgaggctt cgtgggtttt gaagcgggtg ttttccaagc
      181 gtccccagtt gtggtagcgg tacccggcgt ccagggtcag gttgggcgtg atgtcgatgc
      241 ctacgcctgc cactgcgccg aagcccaagc tgctgatgct gcggctttcg tgataagcag
      301 gttgggggac aaaacctgtt gggacagagc ctcccagtaa agcactttgt gtttgtttag
      361 aggtaacaat cgtgtttttg ctttccactg aatgaacctg atgtttaacg tgtccgtagg
      421 cgacgcgcgc gccgatatag ggtttgaatt tatcgttgag tttgaaatcg taaatggctg
      481 ataagccgag agaagaagcg gcgtggaagc tgccgtttcc ctgatgtttt gtttgggttt
      541 ctcggtggtt gtggtttata tcttcagtaa cttttttaat agaagaattg ctttctttcc
      601 actttctgta acgggcataa tctgccgcta tcctccagcc gccgaagtcg tagccgaccg
      661 acaccctggg gtggatggaa tgcgtacgga tgtttctgaa ataatcgctt accgtgctta
      721 ttttgttttt gcctggagcg gttggttccg gataatcgtg ggtaatgcgt tcggcggcgt
      781 aggctaaatc cgcctgcaca tacgggccgc ggccattccc ttcacttgcc gcctgcgcgg
      841 cggaagagaa gagaagagaa gagaagagaa gagaagagaa ggttttttgc gggctggatt
      901 cattttcggc tccttattcg gtttaaccgg ttaaaaaaaa gattttcact gatgcttcaa
      961 aggcggatta tatcgggttc cgggcggtgt ttcaataaga aataataaga aagccgctat
     1021 tatggtttaa tcctgcgtgt tggatttgag ggcaggggct tgcgccattt tgaatcaagc
     1081 cctgccccgc cgccttgaag ggcggggttt aactgttagg aaacaatggc aaagcggctt
     1141 gtttctgtca gacattgtat gggttttctt ttcaatgata tattttcgcc tgtttatgca
     1201 atattttccg attgatggtt tttatcgcga tttttcggca tttgcctttc ggggcggctt
     1261 gtgtttcgtg cgggatgtcg cgtgtcggga tgttcggatt gtcagaagca atatgggaga
     1321 agatgatgta tgaaataaaa cagccttttc atagcggata cttgcaggtg tctgaaattc
     1381 atcaaattta ttgggaagag tcgggcaatc ccgacggtgt gccggtcatt tttttacacg
     1441 gcgggccggg cgcgggggct tcgcctgaat gtcggggttt tttcaatcct gatgtgttcc
     1501 gcatcgtcat catcgaccag cgcggttgcg gacgttcgca cccgtatgct tgtgcggaag
     1561 acaatacgac ttgggatttg gtggcggata ttgaaaaagt ccgtgaaatg ctgggtatcg
     1621 ggaaatggct ggtgttcggc ggttcgtggg gcagcacttt gtcgctggct tatgcccaaa
     1681 cccatcccga acgggtaaag ggattggtgt tgcgcgggat atttttgtgc aggccgtctg
     1741 aaacggcgtg gctgaacgag gcgggcggcg tgagccggat ttatccggaa caatggcaaa
     1801 aatttgtcgc gccgattgct gaaaatcggc ggaaccggct gattgaggcg tatcacgggt
     1861 tgctgtttca tcaagatgaa gaagtgtgcc tgtctgccgc gaaggcttgg gcggattggg
     1921 aaagctatct gattcgtttc gagccggagg gagtggatga agatgcttat gcctcactgg
     1981 caatcgcgcg tttggaaaac cattattttg tcaacggcgg ttggttgcag ggtgataagg
     2041 cgattttgaa caatatcggc aaaatacggc atatcccgac cgttatcgtg caggggcggt
     2101 atgatttgtg tacgcctatg cagagtgcgt gggagctgtc gaaagccttt cccgaagcgg
     2161 aattgagggt ggtccaggcg gggcattgtg cgttcgatcc gcctttggcg gatgcgttgg
     2221 ttcaggcggt tgaggatatt ttgccccgtt tgttgtaaaa agttccgcac aaaaaagcag
     2281 cttctgtttg gaagctgctt ttgttttgaa cggtttaacg cagttcggaa tggagtttgc
     2341 ctaacagtgc ggatgcgtct ttgccggcat atgcgctgcc gtctttgttg agcaggacga
     2401 tgcgcgaacc gtcggcgaca ggctcggcat agacaatcag ttccggctgt tcggcaggtt
     2461 tctccgcttt gcctttgccc agtaggcgtt tgaacagccc cggtttttgt tcggtaactg
     2521 cattgctttc gttcggggct ttttgaacca ggaaggcgtg gcgttcggtg ttttgaccga
     2581 cgacggtcag tccgatgcgg tcgagggcaa ggccggtgcg ccgccagttt ctgccgtagt
     2641 cgccaaagac aatcaggctt ttgccttcga tacgcgccat ttcgttggcg gcgggaaggg
     2701 tcggtttttt tgccaatgcg ttttccgcct gccgtccgtc aacgcccaaa tattgcataa
     2761 agcgcgtcag gaaagcggcc tcaaggttgg ggtcggaagc ggaaggctgc cacatggtcg
     2821 tgtctttgtt tttgtcgcca tacacttctt tcatcgcttt gtgggcgaag aagatgtcgg
     2881 aaacgccgtt tttgccctgt tcgatacgga cgatgaattt gtcgcgctcg ccggtggagt
     2941 agatgccgcc caaaccgact gtgtcgaata ggcggcgcaa gctgtcttgg gggattttgg
     3001 cacggttttc cgcccactcg gtttccattt gtccgatggc gggttcttcg gattcgatgt
     3061 cgaagccgtt ttcctgccaa aaggctttca gaagcggcca gatttcggcg ggggatttgc
     3121 cgtcaacgac aagccagcgt tggctgccgt cgcgctcgag gcggacgcct ttgacgcttt
     3181 tcaatacttc ggcatcggct ggctgttgga cggcgggtgt gcggcgtttt tccaaatcgc
     3241 tggcgcggac ggctcccgaa ccggcaggca ggcggtagag gttgccttgg tcggggttgt
     3301 tcaaatcagg cgggacttcg agtttgatca ggcggtgcga ccggctttgg tagtcgagct
     3361 tgggctgttc ggttttgctg ccggagcagg cggcaagccc gatgagtgcg agcgcggcaa
     3421 tgacgggttt gatatgggtc atcgtgtcat cctgtgtgat ggatattaaa gtgtttgtca
     3481 tgttatgccg tccgaacggt tcggacggca tggctatatt taaagttgtc ctgaggcttt
     3541 cagggcggcg cggacttttg cctgtccgcc ttccgtcagc ggaacgagcg gcaggcggac
     3601 gtgcggtccg catctgccca aggcggatac cgcccatttc ggtgcggcgg gactgggttc
     3661 gcagaacatg gtgtcgtaaa tcggaatcag ccggtcgttg agttcgcgtg caagggcaat
     3721 gttgccttgc agcgcggcac ggcacatatc ggcaaagagc ttgggcgcgg cgttggcggc
     3781 tacggtaata acgccgtgtc cgccgcagag catgaacggc agggcggtgt ggtcgtcgcc
     3841 ggaaaggacg acgaagcctt cgggcgcgcg gttgatgagt tcgatgttgc tgccgacgtt
     3901 gccgcttgct tctttaacgc cgacgatgtt ggggatttcg gcaaggcgca ggatggtttc
     3961 gttgttcatg ctgacgacgg ttctgccggg cacgttgtag agtatcatcg gaaccgcggc
     4021 ggcttcggca acggctttga aatggcggta catgccttct tgggaaggtt tgttgtaata
     4081 ggggacgacg gagagggtgt agtccgcgcc ggctttttcg gcggcttggg aaagggcgat
     4141 ggcttcgacg gtgttgttcg cccctgtgcc ggcgatgacg gggacgcgtt tggcaacgtg
     4201 tttgaccgtt tcttc
//
LOCUS       DAWTQS010000101         4207 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.101, whole genome shotgun sequence.
ACCESSION   DAWTQS010000101 DAWTQS010000000
VERSION     DAWTQS010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.101"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            37..228
                     /gene="mobC"
                     /locus_tag="WNA25_002000"
     CDS             37..228
                     /gene="mobC"
                     /locus_tag="WNA25_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFA9824670.1"
                     /translation="MRVLAGMGNNLNQIAKALNTAAKVGTLGNVEALKATTELAALER
                     SLNSLRDFLAKEKNGWQSQ"
     gene            213..842
                     /locus_tag="WNA25_002001"
     CDS             213..842
                     /locus_tag="WNA25_002001"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFA9824671.1"
                     /translation="MAVPMIVQFFNRGKGGGSGPIDYLLGKDRDREEARLLRGDPEET
                     AALINSSDYAKKYTAGCLSFEESNIPAEQKHALMDSFEECIFAGLDKDQYNCLWVEHR
                     DKGRLELNFVIPNIELLSGKRLQPYYYAADRGRVDAWRTMQNLTHGYSDPDDPAKRQS
                     MTQAKDLPRNTQEAAQSITEHHRRLRSPSPIRQAKKPRRRAGNAGKGRF"
     gene            926..1567
                     /locus_tag="WNA25_002002"
     CDS             926..1567
                     /locus_tag="WNA25_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cryptic plasmid protein B"
                     /protein_id="HFA9824672.1"
                     /translation="MSKISDLAKTFEQTSRTEAASIEQQTKADLETLRKNINEALKQS
                     EQKITADINARQLRMSKAVFKPYLWSLLGISAAGLIVIAGLFIAIWSVKNELDDLKQQ
                     RAEAERTLDLLETKTKGLTLENCPVENSKATRVCVATEKRMLDALAELESNHAAIEQR
                     MMKALTHLGERLAELEQENTSLAQQLASLAAELERQSEIQQRQSEILNQLAKR"
     gene            1599..1868
                     /locus_tag="WNA25_002003"
     CDS             1599..1868
                     /locus_tag="WNA25_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cryptic plasmid protein A"
                     /protein_id="HFA9824673.1"
                     /translation="MGNEPMITENERDRRTAAWLIETYGAEAVAEAETRIAGARKPYP
                     SNIAKVLGASLPEALKRTENAAARQKLAGLRRMLDGKAVKTSQDL"
     gene            complement(1846..2124)
                     /locus_tag="WNA25_002004"
     CDS             complement(1846..2124)
                     /locus_tag="WNA25_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease VapD"
                     /protein_id="HFA9824674.1"
                     /translation="MYAISFDLVVADTAQNHPKGISQAYADIGYTLRKFGFTRIQGSL
                     YTCQNEDMANLFSAINELKALPWFPSSVRDIRAFRIEQWSDFTSLVKS"
     gene            complement(2124..2309)
                     /locus_tag="WNA25_002005"
     CDS             complement(2124..2309)
                     /locus_tag="WNA25_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5397 family protein"
                     /protein_id="HFA9824675.1"
                     /translation="MQTTIPTGSIRTFGDYGVMYIVGTPAEQLPDGDWLVNIELPESG
                     EHTQYKLSHIVQDPKAA"
     gene            complement(2312..2557)
                     /locus_tag="WNA25_002006"
     CDS             complement(2312..2557)
                     /locus_tag="WNA25_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824676.1"
                     /translation="MTRYAINRDALYSAFKDFLYSEINANPALKSATVDDLADIVLAK
                     KWRIFLPDGIKRTTAEKAAQRVLYMTNQPENQTQQEN"
     gene            complement(2554..2796)
                     /locus_tag="WNA25_002007"
     CDS             complement(2554..2796)
                     /locus_tag="WNA25_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFA9824677.1"
                     /translation="MAYPKLKTTKRDVTAKELAKRFGCSTRTVFRAWSQSRADYLAEN
                     SISRDKPWEHFGISRATWYRRGKPMPSETDNQSETA"
     gene            complement(2800..3582)
                     /locus_tag="WNA25_002008"
     CDS             complement(2800..3582)
                     /locus_tag="WNA25_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFA9824678.1"
                     /translation="MLVRSYAAAIKRRYIQVNPPHLRVFMLFDLDYEGAGLAWEDNNL
                     PMPAWAAINRENGGAHLAYALSAPVLTAEYGGRQKALRYLAALEAAYKAKLRGDVGFV
                     SLITKNPEHPHWLTLRGVPDAIRGYDLEYLADFVDLDKFKPYIGRSNVEAVGLSRNCT
                     VFNLVSRWAHKNVLAFKQQGYTVQGWLKEVHYQCMRVNGDFPVPMWEKEVKCISKSIA
                     NWVWYKFDIAASNRRFSELQAHRNSLRKTTINAGRTKIITEL"
     gene            complement(3752..4150)
                     /locus_tag="WNA25_002009"
     CDS             complement(3752..4150)
                     /locus_tag="WNA25_002009"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824679.1"
                     /translation="MGLLSGQSLKLGRADSDDELLCSLFGGTRTCQKGKIPLFLTAFC
                     SKKRHFITAFRRYSMKNSGKNTHAALSELVSCFPQNAALLAGLGRAAALQALPLRLLA
                     SLNCGLENFEIRTPIGLLSDYGYFDIVVSS"
BASE COUNT         1144 a         1039 c         1129 g          895 t
ORIGIN      
        1 aaaaaaaccg tcaaattcca attcccgccc gaagtcgtgc gcgtccttgc aggcatgggt
       61 aacaacctga accaaatagc caaggccctg aacaccgccg caaaggtcgg cacgttgggc
      121 aatgtggaag cactcaaggc gacgaccgag ctggcagcgt tggaacgttc cttaaattcc
      181 ctacgggatt ttttagccaa agaaaagaac ggatggcagt cccaatgatt gtgcagtttt
      241 tcaatagggg gaaaggcggc gggagtggtc cgatagacta tcttctaggc aaagaccgcg
      301 accgagaaga agccagatta ttacgcggcg accccgaaga aaccgccgcc ctgataaaca
      361 gcagcgatta cgccaagaaa tacaccgccg gctgcctgag ctttgaagaa agcaacatcc
      421 ccgccgaaca gaaacacgcc ctgatggaca gcttcgaaga gtgtattttt gcaggcttgg
      481 acaaagacca atacaactgc ctatgggtag aacaccgaga caaagggcgt ttggaactca
      541 acttcgtgat accgaacatc gagcttttga gcggaaagcg gttacagccc tactactacg
      601 ccgccgacag aggaagagtg gacgcatggc gcaccatgca gaacctgacg cacggataca
      661 gcgacccaga cgaccccgcc aaacggcaga gcatgaccca agccaaagac ctgccgagaa
      721 acacgcagga agccgcacag agcatcacag agcatcacag acggcttaga agccctagcc
      781 ctatcaggca agctaaaaag ccgcgcagac gtgctggaaa cgctggaaaa ggtaggtttt
      841 gaaatatcac gagcgaccat cagcagcatc agcatcaaga acccggaccc aaaagggcgc
      901 aacatccgac tgaaaggcgc actgtatgag caagatttcc gatttggcga agaccttcga
      961 gcagacatca cgcaccgaag ccgccagcat agagcaacaa acgaaagcag acttagagac
     1021 gttacggaaa aatatcaacg aggcattgaa gcaaagcgag cagaaaataa ccgccgatat
     1081 aaacgcccgg cagttacgca tgagcaaggc agtattcaag ccctatctgt ggagcttgct
     1141 aggtatatcg gcggcagggt tgatagtcat agcagggctg ttcatagcga tatggagcgt
     1201 caagaacgag ctggacgact tgaaacagca gagagccgaa gcagagcgca ccctagacct
     1261 gttggaaacc aagaccaaag gtttgacact ggaaaattgc ccagtcgaga acagcaaagc
     1321 aacgcgggta tgcgtagcga ccgagaagcg aatgctggac gcgttagcgg aattagagag
     1381 caatcacgca gcaatcgagc agcgaatgat gaaagcctta acgcacttgg gcgaaaggtt
     1441 ggcagagcta gagcaggaaa acacgagttt agcgcagcag ctagcgagct tggcagccga
     1501 gttagagcgg cagagcgaaa tacagcaacg gcagagcgaa atcttgaatc aactagccaa
     1561 acgataagcc aacgacacaa acgaacccaa agcaggggat ggggaatgag ccgatgatta
     1621 ccgagaacga acgcgacagg cgaacagccg catggctgat agagacctac ggggcagaag
     1681 ccgtagcgga agcagaaacc cgcattgcgg gcgcgagaaa gccctatccg agcaatatcg
     1741 ccaaagtatt gggggctagc ctacccgaag ccctaaaacg cacagaaaac gccgcagcgc
     1801 gccaaaaact ggcagggctg cggcggatgt tggacggtaa ggcagttaag acttcacaag
     1861 acttgtgaaa tcagaccact gctcaatgcg gaacgcccga atatcgcgga cagaagacgg
     1921 aaaccaaggc agagctttta gctcgttgat ggctgaaaac aggttagcca tatcttcgtt
     1981 ttggcaggtg tacaaacttc cctgaatgcg ggtaaagccg aatttccgca gggtgtatcc
     2041 aatatccgcg taggcttggg aaatgccttt cggatggttt tgagcggtat cggcaaccac
     2101 caaatcaaaa gaaatcgcgt acattaggca gcctttgggt cttgaacgat gtgggacagt
     2161 ttgtattgcg tgtgttcgcc gctttccggc agttcaatat ttacgagcca atcgccgtcc
     2221 ggaagctgtt cggcaggcgt gccgacgatg tacatcacgc cgtaatcgcc aaaggtgcgg
     2281 attgaaccgg tggggatagt ggtttgcatg gttagttctc ctgttgggtt tggttttcag
     2341 gctggtttgt catgtacaaa acccgttggg cagctttttc ggctgttgtg cgtttgatgc
     2401 cgtcaggcag aaaaattctc cattttttgg ctagaactat atctgcaaga tcatcaaccg
     2461 ttgcgctttt caaagcagga tttgcgttaa tttccgaata cagaaaatct ttgaatgccg
     2521 agtacagagc atcgcggttt attgcgtatc ttgtcatgct gtttcgcttt ggttgtcagt
     2581 ttcagacggc ataggcttgc cgcgcctgta ccaagtggcg cgggaaatgc cgaagtgttc
     2641 ccacggttta tcgcggctga tagagttttc ggctaggtag tcggcgcggg attgcgacca
     2701 tgcccgaaaa acggttcttg tggaacagcc gaagcgtttt gccagttctt tagccgtaac
     2761 gtctcgcttg gtggttttca gttttggata ggccatattt caaagctccg tgatgatttt
     2821 tgttctgcct gcattgatgg ttgttttccg caaactattc cgatgagcct gcaattccga
     2881 aaaccgtcga ttgctggctg caatatcaaa cttgtaccaa acccagttag cgattgattt
     2941 ggagatacat ttcacttctt tttcccacat cgggacaggg aaatccccat ttacccgcat
     3001 acactggtaa tgcacttctt tcagccagcc ttgcaccgtg tagccctgct gtttgaacgc
     3061 caacacgttt ttgtgcgccc aacggctcac aaggttaaac accgtgcaat ttctgcttaa
     3121 tccgaccgct tccacgttag agcgaccgat atagggctta aacttgtcta aatccacgaa
     3181 atccgcaaga tactccaaat cgtagcccct gattgcgtca ggaacgccgc gcagcgtcag
     3241 ccaatgcgga tgttcgggat ttttcgtaat cagcgataca aagcccacat caccgcgcaa
     3301 tttcgcctta tatgctgctt caagtgcagc aagatagcgc agggcttttt gtctcccacc
     3361 gtattccgcc gtcagcacag gcgcggaaag cgcataggca aggtgtgcgc cgccgttttc
     3421 cctgttgatt gccgcccaag caggcatagg cagattattg tcttcccaag ccaaccccgc
     3481 cccttcgtaa tccaagtcaa agagcataaa cacacgcaga tgcggcggat tgacttggat
     3541 gtagcggcgt ttgatggcgg cagcgtaaga gcgcaccagc ataggcgctt ctttgaaatc
     3601 tttgcagtat ggcttgtgtg ggatacgttc ttgcaagaag aggtcgggtt gggtgtataa
     3661 ttggctcatg ttgtatctcg aaacccccgt gcagattggc gtttggcggg ggttttgctt
     3721 tgtctaagat ttgcagattg tatgcttgtt tttaagatga tacaactatg tcaaaataac
     3781 cataatcaga taacagcccg ataggggttc ttatttcaaa attttccaat ccgcaattta
     3841 gcgaagccag caggcgaagc ggtaaagctt ggagcgcagc agcgcgacct aagccggcca
     3901 gcagggcggc gttttggggg aaacatgaaa ccagttccga cagggcggcg tgcgtgttct
     3961 tccctgagtt cttcatggag tatcggcgaa atgccgtgat gaaatgccgt ttttttgagc
     4021 agaaagcagt caaaaacagg ggtattttgc ccttttgaca ggttcgagtg ccgccgaaaa
     4081 gcgaacaaag caactcatca tccgagtcag cccgaccgag tttgagactt tgacccgaca
     4141 gaagacccat ccgaatttag cccgctacat tcgggagcgg gttttggaag atggcaaagc
     4201 atccgac
//
LOCUS       DAWTQS010000102         4176 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.102, whole genome shotgun sequence.
ACCESSION   DAWTQS010000102 DAWTQS010000000
VERSION     DAWTQS010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.102"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            522..1316
                     /locus_tag="WNA25_002010"
     CDS             522..1316
                     /locus_tag="WNA25_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFA9824680.1"
                     /translation="MNTILAFDIETVPDVQGIRTLYDLPSSLPDDEVVLFAQQKRRAQ
                     TGGDFMQHHLHQVVAVSCCMRWGQDKVHVGTIGEMDDGEEVVIAKFFELVEKHTPQLV
                     SWNGGGFDLPVLHYRSLIYGINAARYWDTGDGDFGDSRDFKWNNYISRYHQRHCDLMD
                     LLALYQPRANVPLDDMAKLCGFPGKLGMDGSKVWEAFHAGRLKEIRNYCETDAVNTYL
                     MYLRFCLVSGRFDADEYEMEIKRIRNYLSAQTEDKPHWAEFVQAWK"
     gene            complement(1365..1469)
                     /locus_tag="WNA25_002011"
                     /pseudo
     CDS             complement(1365..1469)
                     /locus_tag="WNA25_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            1541..4144
                     /gene="pepN"
                     /locus_tag="WNA25_002012"
     CDS             1541..4144
                     /gene="pepN"
                     /locus_tag="WNA25_002012"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFA9824681.1"
                     /translation="MSKTVRYLKDYQTPAYRILETELHFDIAEPQTVVKSRLTVEPQR
                     AGEPLVLDGSAKLLSVKINGAAADYVLEGETLTIADVPSERFTVEVETEILPAENKSL
                     MGLYASGGNLFTQCEPEGFRKITFYIDRPDVMSKFTTTIVADKKRYPVLLSNGNKIDG
                     GEFSDGRHWVKWEDPFAKPSYLFALVAGDLAVTEDRFTTMSGRNVKIEFYTTEADKPK
                     VGFAVESLKNAMKWDETRFGLEYDLDIFMVVAVGDFNMGAMENKGLNIFNTKFVLADS
                     RTATDTDFEGIESVVGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSGDRAGRA
                     VRRIENIRLLRQNQFPEDAGPTAHPVRPVSYEEMNNFYTMTVYEKGAEVVRMYHTLLG
                     EEGFQKGMKLYFQRHDGQAVTCDDFRAAMADANGINLDQFALWYSQAGTPVLEAEGRL
                     KNNVFELTIKQTVPPTPDMADKQPMMIPVKVGLLNRNGEAAAFDYQGKRATEAVLLLT
                     EAEQIFLLEGVTEAVVPSLLRGFSAPVHLNYPYSDDDLLLLLAHDSDAFTRWEAAQTL
                     YRRAVAANLAALSDGIGLPKHEKLLAAVEKVISDDLLDNAFKALLLGVPSEAELWDGT
                     ENIDPLRYHQAREALLDTLAVRFLPKWHELDRQAAKQENQSYEYSPETADWRTLRNVC
                     RAFVLRADPAHIETVAEKYGEMAQNMTHEWGILSAVNGNESDTRNCLLAQFADKFSDD
                     ALVMDKYFALIGSSRRSDTLQQVQTALQHPKFSLENPNKARSLIGSFSRNVPHFHAQD
                     GSGYRFIADKVIEIDRFNPQVAARLVQAFNLCNKLEPHRKNLVKQELQCIRAQEGLSK
                     DVGEIVGKILG"
BASE COUNT         1028 a         1120 c         1120 g          908 t
ORIGIN      
        1 taaatttgtt taaaaacata aagttgtaaa caagtatctc atataagcct ttttcattaa
       61 acagatagtc agatattttg tgctaaaaat ttatataata tttaaattaa tatcaagtta
      121 taaaaaatat atggaatttt attttgttta tttataattt taagcaataa tctaaatcag
      181 gcatttttat tcccctgttt taaaaaatat ttggcaaggt gtgaaaaaag gcgtacattc
      241 cgctacacag aattacagat acagcggagc aatgccgtct gaaaggattt tccggtcagt
      301 cttgcggttg gtcggggttt catcggatac ggtgaaacga aagtttgccg gcgcagggtt
      361 gagctacgcg ggtaaagccg caggcgaaag cctgtattgt ttgtgaagcg taaatctctg
      421 atttgaggta ttggggcaat cctgtggggg ttgcctcttt ttttatccgc cttttaatga
      481 cacaataggc gcaagtctgt ttttgaatgc aaggtactgc catgaatacg attttggctt
      541 tcgatattga aaccgtaccc gatgtgcagg gtatccgtac gttgtatgac ttgccgtctt
      601 ccctgccgga tgacgaagtg gtgctgttcg cgcagcagaa acgccgcgca cagaccggcg
      661 gcgattttat gcagcaccac ctccatcagg ttgtggcggt ttcctgctgt atgcgctggg
      721 ggcaggacaa ggttcatgtc ggcaccatcg gcgagatgga cgatggcgag gaagtggtta
      781 tcgccaaatt tttcgagttg gtggaaaaac atacgccgca actggtcagt tggaacgggg
      841 gcgggttcga tttgcccgta ctgcattacc gctccctgat atacggcatc aacgccgcgc
      901 gctattggga tacgggcgac ggcgatttcg gcgacagccg cgatttcaag tggaacaact
      961 acatcagccg ttatcaccaa cgccactgcg atttgatgga tttgctcgcg ctttaccagc
     1021 cgagggcgaa tgtgccgctg gacgatatgg cgaaactgtg cggctttccg ggcaagctgg
     1081 gtatggacgg cagcaaggtg tgggaggcgt tccacgcggg caggctgaag gaaatccgca
     1141 attattgcga aaccgatgcc gtgaatacgt atttgatgta tctgcgtttc tgtctggtca
     1201 gcggcagatt cgacgcggac gaatacgaaa tggaaatcaa gcggatcaga aactatctct
     1261 ccgcccaaac agaagacaaa ccgcattggg cagaatttgt ccaagcgtgg aaatagaaac
     1321 ctgctgtccg aaggcggtac gcttgtgccg ccgtttcggt ttcagacggc gtatggtgga
     1381 ttaaattcaa accggtacag ccttgcctct ccttgcctta ctatctgtac tgtctgcggc
     1441 ttcgtcgcct tgtcctgatt tttgttaatc cgctttaaaa gctgtatccg tgccgtctga
     1501 agccttttcc agtcaaacca aacaatattg aaagaaaaaa atgagcaaaa ccgtccgtta
     1561 tctgaaagat taccaaacgc ctgcctaccg cattcttgaa accgaactgc atttcgacat
     1621 tgccgaaccg caaaccgtcg tgaagtcgcg tttgacggtc gagccgcaga gggcgggcga
     1681 gccgctggtg ttggacggtt cggcaaaact cttgtccgtc aaaatcaacg gcgcggcggc
     1741 ggattatgtg ttggaaggcg agacgctgac gattgcagac gtaccgtccg aacgcttcac
     1801 cgtcgaagtg gaaaccgaaa tcctgccggc ggaaaacaaa tcgctgatgg ggctgtatgc
     1861 ttccggcggc aatctgttta cccagtgcga gccggagggc ttccgcaaaa tcacgttcta
     1921 catcgaccgt ccggatgtga tgtccaagtt cacgaccacc atcgtcgcgg acaaaaaacg
     1981 ctatcccgtt ttgctttcca acggcaacaa aatcgacggc ggcgagtttt cagacggccg
     2041 ccattgggtg aaatgggaag acccgtttgc caaaccgagt tatctgtttg ctttggtcgc
     2101 gggcgatttg gcggtaacgg aagaccgttt caccaccatg agcggcagaa acgtcaaaat
     2161 cgagttttac accaccgaag cggacaagcc caaggtcggc tttgccgtgg aatcgttgaa
     2221 aaacgcgatg aagtgggacg aaacgcgctt cgggttggaa tatgacttgg atattttcat
     2281 ggtcgtcgcc gtaggcgatt tcaatatggg cgcgatggaa aacaagggtt tgaacatttt
     2341 taacaccaag ttcgtcctcg ccgacagccg caccgccacc gataccgatt tcgaaggcat
     2401 tgaatccgtg gtcggacacg aatatttcca caactggacg ggcaaccgcg taacctgccg
     2461 cgactggttc cagctttcgc tgaaggaagg gctgaccgtg ttccgcgacc aagagttttc
     2521 cggcgaccgc gccggccgcg ccgtgcgccg catcgagaac atccgcctgc tgcgccagaa
     2581 ccagttcccc gaagacgcag gcccgaccgc ccatccggtg cgccccgtca gctatgagga
     2641 gatgaacaat ttctacacca tgaccgttta tgaaaaaggc gcggaagtgg tgcggatgta
     2701 tcataccctg ctcggcgaag agggcttcca aaaaggcatg aagctatatt tccaacgcca
     2761 cgacggacag gcagtgacct gcgacgattt ccgcgcggcg atggcggatg cgaacggcat
     2821 caatctcgac cagttcgcct tgtggtacag ccaggcgggc acgcccgttt tggaagccga
     2881 aggccgtctg aaaaacaatg ttttcgagtt aaccattaaa caaaccgtgc cgcccacgcc
     2941 cgatatggcg gacaaacagc cgatgatgat tcccgtcaaa gtcgggcttc tgaaccgcaa
     3001 cggcgaagcg gcggcattcg attatcaggg caaacgcgca accgaagccg tattgctgct
     3061 gaccgaagcc gaacagatct tcctgctcga aggcgtaacc gaagccgtcg ttccctcgct
     3121 gctgcgcggg ttcagcgcgc cggtgcatct gaactatccg tacagcgacg acgacctgct
     3181 gcttctgctc gcccatgaca gcgacgcctt cacgcgctgg gaagccgccc aaacgctcta
     3241 ccgtcgcgcc gtcgccgcca accttgccgc actttcagac ggcatcgggt tgccgaaaca
     3301 cgaaaaactg cttgccgccg tcgaaaaagt catttcagac gacctcttgg acaacgcctt
     3361 caaagccctg cttttgggcg tgccgtccga agccgaactg tgggacggca cggaaaacat
     3421 cgacccgctg cgctaccatc aggcgcgcga agccttgttg gatacgcttg ccgtccgctt
     3481 cctgccgaaa tggcacgaat tggaccgtca ggcggcgaag caggaaaacc aaagttacga
     3541 atacagcccc gaaaccgccg actggcgcac gctgcgcaac gtctgccgcg ccttcgtcct
     3601 gcgcgccgac cccgcgcaca tcgaaactgt tgccgaaaaa tacggcgaaa tggcgcaaaa
     3661 catgacccac gaatggggca tcctgtccgc cgtcaacggc aacgaaagcg atacgcgcaa
     3721 ctgcctgctg gcgcagtttg ccgacaagtt ttcagacgac gcgctggtga tggacaaata
     3781 tttcgccctt atcggctcaa gccgccgcag cgacaccctg caacaggttc aaaccgcctt
     3841 gcagcatccg aaattcagtc tcgaaaaccc caacaaagcc cgttcgctca tcggcagctt
     3901 cagccgcaac gtcccgcatt ttcacgcaca agacggcagc ggctaccgct tcatcgccga
     3961 caaagtcatc gaaatcgacc gcttcaaccc gcaggtcgcc gcccgcctgg tgcaggcgtt
     4021 caacctctgc aacaagctcg agccgcaccg caaaaacttg gtgaaacaag aattgcagtg
     4081 cattcgggcg caggaaggat tgtcgaaaga cgtgggcgaa atcgtcggca agattttggg
     4141 ttgaggcagt caaacagaaa aaacaagggt ctgtac
//
LOCUS       DAWTQS010000103         4132 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.103, whole genome shotgun sequence.
ACCESSION   DAWTQS010000103 DAWTQS010000000
VERSION     DAWTQS010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.103"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            <1..1016
                     /locus_tag="WNA25_002013"
     CDS             <1..1016
                     /locus_tag="WNA25_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFA9824682.1"
                     /translation="AEPHVPVSIPTATPLPAGEVTLSSDNGNIENINTAGAGSASDAP
                     SRSRRSLDAAPQNTSGISIRQREVEKDYFGYKSKETSFIFKTPGGAQYALSSYADPIT
                     VSYSSPDFKIPDRHAGQRLADGSRIFICCSDSGATSYAEITKQDYMKFGAWIGPNGEI
                     DLFAGGFPVGKTPPPAFSYGSSTPETALSKGKITYQVWGIRVRNGQFVTSSYTPPKSG
                     SYYGTLANTPVLSFITANFNSNTLAGKILGNSDYGPDVDIQNATITGPTFSGDATSGG
                     KSGKLEGKFFGKFASTRSSEVSIGGKITFDGDRSLDTVFGGVSYEKKLDDTSQDTNHL
                     TKQ"
     gene            1044..3473
                     /gene="hupB"
                     /locus_tag="WNA25_002014"
     CDS             1044..3473
                     /gene="hupB"
                     /locus_tag="WNA25_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein HupB"
                     /protein_id="HFA9824683.1"
                     /translation="MPIPFKPVLAAAAIAQAFPAFAADPAPQSAQTLNEITVTGTHKT
                     QKLGEEKIRRKTLDKLLANDEHDLVRYDPGISVVEGGRAGSNGFTIRGVDKDRVAINV
                     DGLAQAESRSSEAFQELFGAYGNFNANRNTSEPENFSEVTITKGADSLKSGSGALGGA
                     VNYQTKSASDYVSEDKPYHLGIKGGSVGKNSQKFSSITAAGRLFGLDALLVYTRRFGK
                     ETKNRSTEGDVEIKNDGYVFDPANPSPSRYLTYKATGVARSQPDPQEWVNKSTLFKLG
                     YNFNDRNRIGWIFEDSRTDRFTNELSNLWTGTTTSAATGDYRHRQDVSYRRRTGVEYK
                     NELEHGPWDSLKLRYDKQRIDMNTWTWDIPKNYDTNGINGEVYHSFRHIRQNTAQWTA
                     DFEKQLDFSKAVWAAQYGLGGGRGDNANSDYSYFAKLYDPKILASNQAKITMLIENRS
                     KYKFAYWNNVFHLGGNDRFRLNAGIRYDKNSSSAKDDPKYTTAIRGQIPHLGSERAHA
                     GFSYGTGFDWRFTKHLHLLAKYSTGFRAPTSDETWLLFPHPDFYLKTNPELKAEKAKN
                     WELGLAGSGKAGSFKLSGFKTKYRDFIELTYMGVSSDDKNNPRYAPLSDGTALVSSPV
                     WQNQNRTAAWVEGIEFNGTWNLDSIGLPKGLHTGLNVSYIKGKATQNNGKETPINALS
                     PWTAVYSLGYDAPSKRWGVNAYAARTAAKKPSDTVHSNDDLNKPWPYAKHSKAYTLFD
                     LSAYLNIGKQVTLRAAAYNITNKQYYTWESLRSVREFGTVNRVNNKTHAGIQRFTSPG
                     RSYNFTIEAKF"
BASE COUNT         1109 a         1279 c          963 g          781 t
ORIGIN      
        1 tagccgaacc gcacgtcccc gtgtccatcc ccaccgccac gccgctgccc gccggcgagg
       61 taacgttatc aagcgataac ggcaatatcg aaaacatcaa caccgccggc gccggaagcg
      121 catccgacgc gccgagccgc agcagacgct cgctcgatgc cgccccgcaa aacacatccg
      181 gcatttccat tcggcagcgg gaagtagaaa aagactattt cggctataaa tcaaaagaaa
      241 catcattcat cttcaaaaca cccggcggcg cgcaatacgc cctttcatcc tacgccgacc
      301 ccataaccgt cagctactcc tcccccgatt tcaaaattcc cgaccgccac gcagggcagc
      361 ggctcgccga cggcagccgc atctttatct gctgcagcga ctccggggct acttcgtatg
      421 cggaaattac caaacaagat tatatgaaat tcggggcgtg gataggtccc aacggcgaaa
      481 tcgacctctt cgccggcggc ttccccgtcg gcaaaacgcc gcctcctgcg ttctcctacg
      541 gcagcagcac gcccgaaacc gcattgtcaa agggtaaaat cacttatcag gtttggggca
      601 tccgcgtcag aaacgggcaa tttgttactt cctcttatac gccgccgaaa agcggttctt
      661 attatgggac acttgccaat acccccgtcc tttcctttat caccgccaat ttcaacagca
      721 acacactggc aggcaaaatc ctcggcaaca gcgattacgg gccggatgtg gatattcaaa
      781 acgcaacgat taccggtccg accttttccg gcgatgccac gtccggcggg aaaagcggca
      841 aattggaagg caagtttttt ggcaagttcg ccagcacccg cagcagcgaa gtcagcatag
      901 gcggcaaaat cacctttgac ggcgaccgct ccctcgatac cgtattcggc ggtgtgagtt
      961 atgaaaaaaa acttgatgac accagtcaag ataccaacca ccttactaaa caataaacca
     1021 cctcatccct gcaaaggttt cttatgccca ttcccttcaa acccgtattg gctgccgccg
     1081 ccatcgccca agcgtttccc gcctttgcgg cagaccccgc gccgcagtcc gcccaaacgc
     1141 tgaacgaaat caccgttacc ggcacgcaca aaacccaaaa actcggcgaa gaaaaaatcc
     1201 gccgcaaaac cttagacaag ctcttggcca acgacgaaca cgacctggtg cgctacgacc
     1261 ccggcatttc cgtcgtcgaa ggcggcaggg cgggttctaa cggctttacc atacgcggcg
     1321 tggacaaaga ccgcgtcgcc atcaacgttg acgggctggc gcaggcggaa agccgctctt
     1381 ccgaagcctt ccaagaattg ttcggcgcgt acggcaactt caacgccaac cgcaacactt
     1441 ccgagccgga aaacttttcc gaagtaacca tcaccaaagg cgcggactcg ctcaaatccg
     1501 gcagcggcgc attgggcggc gcagtcaatt accaaaccaa atccgcaagc gactatgttt
     1561 ccgaagacaa gccctaccat ttggggataa agggcggcag cgtcggcaaa aacagccaaa
     1621 aattcagcag cattaccgcc gccggcaggc tctttggttt ggatgcctta ttggtttata
     1681 cccgccgctt cggcaaagaa accaaaaacc gctcgaccga gggcgatgtc gaaattaaaa
     1741 acgacggata tgtctttgac ccggccaacc cctcgcccag cagatacctt acctacaaag
     1801 ccacaggggt tgctcgctcc caacccgacc cgcaagaatg ggtaaacaaa agcaccctgt
     1861 tcaagctggg ctacaatttc aacgaccgca accgcatcgg ctggattttt gaagactcgc
     1921 gcaccgaccg ttttaccaac gagctgtcta atttgtggac gggtacgacc acgtctgccg
     1981 caacgggcga ctaccgccac cgccaagacg tgagctaccg ccgccgcacc ggcgtcgaat
     2041 acaaaaacga attggaacac ggcccgtggg acagcctcaa gctgcgctac gacaagcagc
     2101 gcatcgatat gaacacttgg acttgggaca tcccgaaaaa ttacgataca aacggcatca
     2161 acggcgaggt ttaccattcg ttccggcata tccgccaaaa caccgcgcaa tggactgccg
     2221 attttgaaaa acaactcgac ttttccaaag ccgtttgggc ggcgcaatac ggcttaggcg
     2281 gcggcagagg ggacaatgcc aactcggatt acagttattt cgcaaaactg tacgacccca
     2341 aaatcctcgc ttccaaccaa gccaaaatca cgatgctgat cgaaaaccgg tcgaaatata
     2401 aatttgccta ttggaacaat gtgtttcact tgggcggcaa cgaccgcttc cgcctgaatg
     2461 cgggcatacg ctacgacaaa aacagcagca gcgcgaaaga cgatccgaaa tacaccaccg
     2521 ccatccgggg gcagattccc catttgggtt cggaacgcgc gcacgcgggc ttcagctacg
     2581 gcacagggtt cgactggcgg tttaccaagc atctgcactt gttggcaaaa tacagcaccg
     2641 gcttccgcgc accgacttcg gacgaaacct ggctgctgtt cccacacccc gatttctacc
     2701 tgaaaaccaa tcccgaactg aaagcggaaa aagccaaaaa ctgggaattg ggcctggcgg
     2761 gcagcggcaa agctggcagc ttcaagcttt cgggcttcaa aaccaaatac cgcgacttta
     2821 tcgaattgac gtatatgggc gtttcgtcag acgataaaaa caaccccaga tacgccccgc
     2881 tttcagacgg cacggcattg gtcagctcgc ccgtttggca aaaccaaaac cgcaccgccg
     2941 cctgggtgga aggcatagag tttaacggca cttggaacct cgacagcatc gggctgccca
     3001 aagggctgca caccggcctc aacgtcagct acatcaaggg caaggcaacg caaaacaacg
     3061 gcaaagaaac gcccatcaac gcgctttcgc cgtggacggc ggtttacagc ctgggctatg
     3121 acgcgccttc caaacgctgg ggcgtcaacg cctacgccgc gcgcaccgcc gccaaaaagc
     3181 cgtccgacac cgtccacagc aacgacgact tgaacaaacc gtggccttat gccaaacaca
     3241 gcaaggccta tacgctgttc gacctttccg cctacctcaa catcggcaaa caggttacgc
     3301 tccgcgccgc cgcgtacaac attaccaaca agcagtacta cacttgggaa tctttacgca
     3361 gcgtccgcga gttcggcacg gtcaaccgcg tcaacaacaa aacccacgcc ggcatccaac
     3421 gctttacctc gccgggcagg agctacaatt tcaccatcga agcgaagttc taagctcctg
     3481 atgtgccaat gccgtctgaa acccgatgat ttgggtttca gacggcattt tttacggtcg
     3541 caccgccgcc ccctttcagg cttgtaccaa gcccaatccc gcaactctcc gtcattcccg
     3601 caactctccg tcattcccag ggaagtggga atctagaaac gcaaagccac aggaacttat
     3661 ccgaaacaac agcaacctaa aattcgtcat tcccacgaaa gtgggaatcc agttcgttga
     3721 gtttcagtta tttaaaataa attttgaaac tttaatccca tcattcccgc gaaagcggga
     3781 atccggaacg taaaatctaa agaaaccgtt ttatccgata agtttccgta ccgacggggc
     3841 tggattcccg cctgcgcggg aatgacggcg gagcggtttc tgttttttcc gataaattcc
     3901 taaaacttaa aatttcatca ttcccacaag gacagaaaac caaaaacaga aacctaaaat
     3961 tcgtcattcc cgcaaaagcg ggaatccagt tcgttgagtt tcagttattt agaataaatt
     4021 ttgaaacttt aatcccgtca ttcccgcgaa agcgggaatc cggaacgtaa aatctgaaga
     4081 aaccgttttc ccgataagtt tccgtgccga cggggctgga ttcccgcctg cg
//
LOCUS       DAWTQS010000104         3916 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.104, whole genome shotgun sequence.
ACCESSION   DAWTQS010000104 DAWTQS010000000
VERSION     DAWTQS010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.104"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(1..128)
                     /locus_tag="WNA25_002015"
                     /pseudo
     CDS             complement(1..128)
                     /locus_tag="WNA25_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223267.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            314..598
                     /locus_tag="WNA25_002016"
     CDS             314..598
                     /locus_tag="WNA25_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HFA9824684.1"
                     /translation="MKLLAALIPLLMSVAGRILTALGLMAVTYAGVDRLAAHFQQAIT
                     HSITGAPQAMLQLFYISGGGTVLNILFGAIAFILSFKQMTKLATSIGKKK"
     gene            599..1786
                     /locus_tag="WNA25_002017"
     CDS             599..1786
                     /locus_tag="WNA25_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2075 domain-containing protein"
                     /protein_id="HFA9824685.1"
                     /translation="MAEICLITGTPGSGKTLKMVSMMANDEMFKPDENGVRRKVFTNI
                     KGLKIPHTHIETDAKKLPKSTDEQLSAHDMYEWIKKPENVGAIVIVDEAQDVWPARSA
                     GSKIPENVQWLNTHRHQGIDIFVLTQGPKLLDQNLRTLVKRHYHIAANKMGLRTLLEW
                     KVCADDPVKMASSAFSSIYTLDKKVYDLYESAEIHTVNKVKRSKWFYALPVIILLIPL
                     FVGLSYKMLGSYGKKQEEPAAQESAATEQQAVLPDKTEGESVNNGNLTADMFVPTLPE
                     KPESKPIYNGVRQVRTFEYIAGCIEGGRTGCTCYSHQGTALKEVTELMCKDYVKNGLP
                     FNPYKEESQGQEVQQSAQQHSDRAQVATLGGKPQQNLMYDNWEERGKPFEGIGGGVVG
                     SAN"
     gene            2001..2946
                     /locus_tag="WNA25_002018"
                     /pseudo
     CDS             2001..2946
                     /locus_tag="WNA25_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694155.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISNgo3 family transposase"
     gene            3249..3788
                     /locus_tag="WNA25_002019"
     CDS             3249..3788
                     /locus_tag="WNA25_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02594 family protein"
                     /protein_id="HFA9824686.1"
                     /translation="MTELPWIAEARRHIGLKEIPGAKHNPTIVQWLKETGGFPGAAKS
                     WYFEDETPWCGLFVGHCLGKAGRAVIRDWYRAKAWSMSGLTKLEAPAYGCIAVKPRRG
                     GGHVFFVVGKDAEGRILGLGGNQGNMVSIIPFDPADIDGYFWPSKLIGGKPVPSSPAE
                     GRYRLSDVAATAKQGAGEA"
BASE COUNT         1266 a          870 c          935 g          845 t
ORIGIN      
        1 ccaagggaaa aagaagcccc agctctgaaa tcatcacctt tattgaccgc acaacctccg
       61 ccgttccaat caaatgtgca acgatttaaa acaaaattat tccaatccaa ttaagcaact
      121 ttagccaaag gcaggcgaag catcgcactt tgcgggcgaa gccgcaaaca gccgagaagc
      181 gcgggggggg attggcgata agcgcgaggg ggtgtcccca cagcgctgcc gcgccgcgaa
      241 tgcggagcaa tctttcagat taagaaacat ttgtttaatg aggcaaacgt gccttttaag
      301 aaagggagag caaatgaaat tgttggccgc attgattccg ctcttgatga gcgtcgcagg
      361 ccgtatattg actgcattag gcttgatggc tgttacctat gcgggtgtag atagattggc
      421 agcccatttt caacaggcga taacccatag cataacgggc gcgcctcaag caatgttaca
      481 gcttttctat ataagcggcg gtggtactgt tctaaacatt cttttcggcg caatcgcctt
      541 tattctgtcc tttaaacaaa tgacaaaact cgcaacctca atcgggaaga aaaaataaat
      601 ggcagaaatc tgtttgataa ccggcacgcc cggttcaggg aaaacattaa aaatggtttc
      661 catgatggca aacgatgaaa tgtttaagcc agatgaaaac ggcgtacgcc gtaaagtatt
      721 tacgaacatc aaaggtttga agataccgca cacccacata gaaacagacg caaagaagct
      781 gccgaaatca accgatgaac agctttcggc gcatgatatg tatgaatgga tcaagaagcc
      841 tgaaaacgtc ggcgcaatcg ttattgtcga tgaggcgcaa gacgtatggc ccgcacgctc
      901 cgcaggttcg aaaatccccg aaaacgtcca atggctgaac acacacaggc atcagggcat
      961 agatatattt gtattgacac aaggtcctaa actcttagat cagaacttgc gaacattggt
     1021 taaaagacat taccacattg cggccaacaa aatgggtttg cgtaccctgc ttgaatggaa
     1081 agtatgcgcg gatgacccgg taaaaatggc atcaagtgca ttttccagta tctacacact
     1141 ggataaaaaa gtttatgact tgtacgaatc cgcagaaatt cacacggtaa acaaagtcaa
     1201 gcgttcaaaa tggttttatg cattgcccgt catcatatta ttgattccgc tatttgtcgg
     1261 tttgtcttac aaaatgttgg gcagttacgg aaaaaaacag gaagaacccg cagcacaaga
     1321 atcggcggca acagaacagc aggcagtact tccggataaa acagaaggag aatcggtgaa
     1381 taacggaaac cttacggcag atatgtttgt tccgacattg cccgaaaaac ccgaaagcaa
     1441 gccgatttat aacggtgtaa ggcaggtaag gacctttgaa tatatagcag gctgtataga
     1501 aggcggaaga accggatgca cctgctattc gcatcaaggg acggcattga aagaagtgac
     1561 ggagttgatg tgcaaggact atgtaaaaaa cggcttgccg tttaacccat acaaagaaga
     1621 aagccaaggg caggaagttc agcaaagcgc gcagcaacat tcggacaggg cgcaagttgc
     1681 caccttgggc ggaaaaccgc agcagaacct aatgtacgac aattgggaag aacgcgggaa
     1741 accgtttgaa ggaatcggcg ggggcgtggt cggatcggca aactgaagaa aacggcaaca
     1801 gagaaaaaag acccgtaaac cgtttgaata tagacggctt acgggtcttt gtttcgcgca
     1861 aagcaagggc taaggcagtc aggcagcaaa tcccgcaatg tattaaaaca gacgcgtaga
     1921 aatgccggct gcctttatcc atcctcaaaa ttgaatatca tcctagccgt atcaaggctg
     1981 tataaataag gaaaatacca atgaatataa tcgggtcgga catctcaaag gacaccatag
     2041 acgcaacatt gcataaaaca aacggaagta tccattacat taaatttaag aataatgatg
     2101 atggattaaa acagtttaga ttgtggataa agggaaacag aatcagaaaa gtctatatcg
     2161 gcatggaggc aacaggcatc tattacgaaa aggcagcaga tatgctttct tcctactata
     2221 ccgtttacgt tatcaatccc ttaaaaatca aggactacgg aaaaagcagg tttaaccgca
     2281 ccaaaaccga caaagcagat tcaaacctga tagcagatta cataaaaagg catcaagata
     2341 cattgatacc gtatcagata cccaaaaaca aagcactgca aaaactgatt aatcttaaaa
     2401 accaattaca gcaacagcag aagcaaatta aaaaccgtct tcatagcact gaagaagact
     2461 tcataaggaa catacatcaa gacttgatag ataccataca ggacaagatg gaacaggtaa
     2521 aaaatagcca tatccgaaca aatcaaaaaa caaacggaca ataaccatta ccgcaatctt
     2581 caaaccatcc cgagcatagg caaagacacc gcatcagttc tttatgcgca actgacagaa
     2641 aaacatttta aaaccgcaaa ccaatttgta tcctatgccg gattaagtcc cgccatcata
     2701 caatcaggga caagcgtaag aggtcggggc agattgagcc gatacggaaa cagacgatta
     2761 aaaagtacgc tgtatatgcc cgccctttgt gcttaccgtt ttaacgcatt tccgaaatta
     2821 ataaataatc tgaaaaaagc gggtaagcca aagatggtaa ttatcgttgc catcatgcgc
     2881 aaactggcga agcccgccta ttacattgtt aaaaccgggc agccttacga tgcggaaaga
     2941 cactgattga atcaataaaa ttcaataaaa ttaaacagtt atgcaaatat atctttgtaa
     3001 ccgtgcattt gcatatcgta aataaacgta aataaaaata acaatataaa tcagcatgtt
     3061 gcaactttgt tttttatttt gtgttgacgg gcaacatatc atctgcgcgg aaagttaccg
     3121 tatcggcaaa cgcgactgaa aaagacagcg tgttcggcga tgcggtgcac attgtgattg
     3181 cgacttaaaa catagaacga gttgccaacc caaagtttac atctcgtctt ttaccgaaag
     3241 gattaaaaat gacagaatta ccgtggatag ccgaagcgag aaggcacatc ggtttgaaag
     3301 aaattcccgg cgcgaaacac aatccgacga ttgtgcaatg gctcaaagag acgggcggct
     3361 tccccggcgc ggcaaagtct tggtattttg aagacgaaac gccgtggtgc ggcctgtttg
     3421 tcggacactg cctgggcaaa gcgggacgcg cggtcatcag ggactggtat cgcgccaaag
     3481 cctggtcaat gtcgggtttg acgaaactcg aagcccccgc atacggctgc atcgcggtca
     3541 aaccgcgccg gggcggcgga cacgtgttct tcgttgtcgg caaagacgcg gaaggcagaa
     3601 tcttgggctt gggcggcaat cagggcaata tggtatccat catcccgttt gaccctgcgg
     3661 acattgacgg ctacttctgg ccgtccaagc tgattggcgg caaacctgtg ccttcgtccc
     3721 ccgccgaagg gcgttaccgg ttgtcggacg ttgccgccac ggcgaaacag ggcgcgggcg
     3781 aggcgtaaat gattggggct ttgctgaaaa attggaagcc gctgcttatt ttgtccgcaa
     3841 tcgcgttctt cgccgtttct tggcagctgg acagggcggc gcaataccgt cgcggatacg
     3901 gtgcggcggt gtcgga
//
LOCUS       DAWTQS010000105         3868 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.105, whole genome shotgun sequence.
ACCESSION   DAWTQS010000105 DAWTQS010000000
VERSION     DAWTQS010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3868
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.105"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(118..405)
                     /locus_tag="WNA25_002020"
     CDS             complement(118..405)
                     /locus_tag="WNA25_002020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824687.1"
                     /translation="MSYLDQPLKHGYGNGNGSGNGSGYGNGYGNGYGNGYGGVGGSGS
                     GSGYGNGKGYSNGSGNGDGDGNGNGSGNGSGNGDGDGNGNGSGNGSGYGNG"
     gene            complement(402..878)
                     /locus_tag="WNA25_002021"
     CDS             complement(402..878)
                     /locus_tag="WNA25_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824688.1"
                     /translation="MEANKFEVKSLSDLIKVFAGIAADFEAAMGVKRADISTEFDEPQ
                     HEPQPPVTVAEQKGINDFAIGKEVIIRTYSAGVWFGVLKQKAGNEVILTKARRMYSWW
                     AKESISLSGVARHGIRQDGSQICGELDSVWLEAIEIIPVTGGAAESIRTALEVAQS"
     gene            complement(911..1111)
                     /locus_tag="WNA25_002022"
     CDS             complement(911..1111)
                     /locus_tag="WNA25_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824689.1"
                     /translation="MNELISRINRVGARAKDGQSLLLKVGEICRDAAATWTTRKSESI
                     NHTAFTFTVKKDGLKEKVMIVL"
     gene            complement(1309..1722)
                     /locus_tag="WNA25_002023"
     CDS             complement(1309..1722)
                     /locus_tag="WNA25_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824690.1"
                     /translation="MIHLNVHYCDEIIRHAENDKYSLIGIFPDICHIPTPQAILGRLC
                     LSVSFSAEGMDIQTMKTGQIFLEIVRNDDVISALEIPSYDGSDTEENVSFMLHQTISG
                     LPVSDNDRIYVRMTTHNHILSESRPLSFSWLPYHS"
     gene            complement(1719..2180)
                     /locus_tag="WNA25_002024"
     CDS             complement(1719..2180)
                     /locus_tag="WNA25_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFA9824691.1"
                     /translation="MGATLTVAVAACTVSTPTSAIPVTHIKCLRINGQIKCVKPISPN
                     TTPAAEHIEHVRKNPRRKAAMDRAAARIADKIALKAGGETFVSLRMKKGFTQSELATA
                     AGLPQPYLSRIENSKQSLQDKTVQKLANALGVSPLEVRAAFERRYEYMEQA"
     gene            complement(2197..2634)
                     /locus_tag="WNA25_002025"
     CDS             complement(2197..2634)
                     /locus_tag="WNA25_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824692.1"
                     /translation="MELTVHFNAEQDLDRLFEKDEEAVGYLENVIAMIQADSAIFDGL
                     YKNRYFREYGEPIGPIDLEVKPILSLWGKDIKVLRVRFDSEEAAGYRIIYAPCHEKQP
                     NGTYIRRIDILAVVNKKTDEFDYQAEHPITKRIIKDYEELYSN"
     gene            complement(2747..3463)
                     /locus_tag="WNA25_002026"
     CDS             complement(2747..3463)
                     /locus_tag="WNA25_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HFA9824693.1"
                     /translation="MPFIIDYQCLCAINSEIKMFSGEQLGQAISEAIKRKNVSQKEVA
                     DHFGVKQPSVSGWIKNGRIDKKHLDKLIDYFSDVVTPSHFGIETFRVLKSNEQSSIRF
                     PRLNAEATCGAGTINDHYIEVVDYVTVAAAWAREKLGGNLNKIQVITARGDSMEPTIE
                     NGDVMFVDTAVEAFDGDGLYLLWYIDGLKAKRLQSTVGGGLMIISDNSSYRTETVRGE
                     DLNAVRIIGRIRGAWRLSQF"
     gene            3538..3768
                     /locus_tag="WNA25_002027"
     CDS             3538..3768
                     /locus_tag="WNA25_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFA9824694.1"
                     /translation="MSIQKAVDYFGNESRLARAIGVKQPTVWAWNKKGTPPPIIRCVQ
                     IEKLTGGAVNRKDLRPDDWHLIWPELAGDQPK"
BASE COUNT          866 a          916 c          932 g         1154 t
ORIGIN      
        1 aaaccgcccg cagcattcac tgtttcgccg tgccgttgcc ccgctttgaa gttcgatact
       61 tcatcgcttt gtgctatccc cggcttggca gatatagctt tcgggcggtt ttaaggttta
      121 gccgttgcca tagccgctac cgttgccgct accgttgccg ttgccgtcgc cgtcgccgtt
      181 gccgctaccg ttgccgctac cgttgccgtt gccgtcgccg tcgccgttgc cgctgccgtt
      241 gctgtagccc ttgccgttgc cgtagccgct gccgctgccg ctaccgccga cgccgccgta
      301 gccgttgccg tagccgttgc cgtagccgtt gccatagccg ctaccgttgc cgctaccgtt
      361 gccgttgccg tagccgtgct tcaatggttg atctagataa ctcatgactg ggcgacctcc
      421 agcgcggtgc ggattgattc agccgcgccg cctgttactg ggataatctc aatcgcctcg
      481 agccatacgg aatcaagctc gccgcaaatt tggctgccgt cttgcctgat gccgtgtcgt
      541 gcgacacctg acaggctgat tgattccttt gcccaccagc tgtacattcg gcgcgctttt
      601 gtcagaatca cttcattgcc tgctttttgt ttcaacacac caaaccaaac gcctgccgaa
      661 taagtgcgga tgatgacttc cttgccgatg gcaaagtcgt tgataccttt ttgctcggca
      721 actgttaccg gcggttgcgg ctcatgttgc ggttcgtcaa attcggttga aatgtcggcg
      781 cgttttacac ccattgccgc ttcgaaatcg gcagcaatgc ctgcaaagac ttttataagg
      841 tctgacaaac ttttcacttc aaatttattt gcttccattt ttgtttcctt tcggggtggg
      901 gttggtttct ttacaaaaca atcattacct tctcttttaa gccgtctttt ttcactgtaa
      961 aagtgaaggc ggtgtgattg atgctttcgc tttttctagt ggtccatgtc gctgctgcgt
     1021 cgcggcagat ttcaccaact ttcaataaaa ggctttgccc gtcctttgcc ctcgcgccga
     1081 cccggtttat tctgctgatt aattcgttca tcccgtttcc tttaagtggt tgtttgtttc
     1141 gatgggtgta ttataactat tacctatatt taatcaatag gcaatagtta tattttcatc
     1201 catatatact tattgaattg atttttagaa gaaaaaagtt tgaaaaaaaa ccgcccgaat
     1261 ggcggtgtat catgaaaaag aaaaccgccc tagggcggtt cggtattgtt atgagtggta
     1321 aggaagccaa gaaaaagaca aaggacggct ctcggagaga atatggttgt gtgttgtcat
     1381 tcttacataa atgcggtcgt tgtctgaaac gggtagtccg ctgattgttt ggtgtagcat
     1441 gaatgacacg ttttcttctg tatcactgcc gtcatatgat ggaatctcaa gtgcagagat
     1501 tacatcatcg ttgcggacga tttccagaaa aatttgaccg gttttcatag tctgtatatc
     1561 catgccttcg gcggaaaatg aaacagacag gcacaggcgg cctagaatgg cctgcggtgt
     1621 cggaatatgg catatatcgg ggaatatacc tatcaggctg tatttgtcat tttccgcatg
     1681 gcggataatt tcatcgcaat agtggacatt taagtgaatc atgcctgctc catatattcg
     1741 taccgccgtt cgaacgctgc gcgaacttca agcggggaaa cgcctaatgc gtttgccaat
     1801 ttttgtacag tcttatcttg cagggattgt ttgctgtttt cgatgcggga cagatagggt
     1861 tgaggcaatc ctgcggcggt tgccaattcg gactgggtaa accccttttt catccttaag
     1921 ctgacaaatg tttccccgcc tgctttgagg gctattttat cggcaattct tgctgccgcc
     1981 ctgtccatcg ctgctttgcg gcgcggattt ttccgcacat gctcaatatg ctctgcggca
     2041 ggggtagtgt tagggcttat cggtttgacg catttaatct gcccgtttat ccgcaggcat
     2101 tttatgtggg tgacggggat ggcggatgta ggtgttgata ctgtgcaggc ggccacggca
     2161 acagtgagtg ttgcacccat tttgaaccat gtgccgttag ttggagtaca gttcttcata
     2221 gtctttgata atccgttttg tgattgggtg ttctgcctga tagtcaaatt cgtctgtctt
     2281 cttgtttaca acagccaata tatctatgcg gcggatatac gtgccgtttg gctgtttttc
     2341 atggcatggt gcgtaaatta ttctgtatcc ggcggcttct tcgctgtcaa atcggacgcg
     2401 taaaactttg atgtcttttc cccataatga caatatgggt ttcacttcca agtcaatcgg
     2461 gcctatgggt tcgccatatt ccctgaagta tctgtttttg tataagccgt caaaaatagc
     2521 agagtccgcc tgaatcatcg caatgacatt ctcgagataa ccgaccgctt cttcgtcttt
     2581 ttcaaagagg cggtctaaat cctgctcggc gttaaagtgg acggtcagct ccatgagtgc
     2641 cctttttata tatatattat atctttttag ttataccgcg caatatgcgg taataaatca
     2701 gttgttcaag tattttaatc cggcacgctc caccagaaga cgcggtctag aactggctca
     2761 aacgccatgc gccgcgtatg cgtccgatga tgcgtacggc gtttaaatct tcgccgcgca
     2821 cggtttcggt tcggtatgag ctgttgtcgc tgatgatcat caggccgccg ccgacggtgg
     2881 attgcagccg cttggcctta aggccgtcta tataccaaag caggtagagg ccgtcgccat
     2941 cgaaggcttc gacggcggta tcaacgaaca ttacgtcgcc gttttcgatg gtgggctcca
     3001 tgctgtcgcc acgggctgta atgacttgga ttttgttgag gtttccgccc agtttctccc
     3061 gcgcccatgc ggcagcgacg gttacataat ccacaacctc gatatagtgg tcgttaatcg
     3121 tgcctgcgcc gcaggtcgct tcggcattta agcgggggaa acgtatgcta ctttgttcat
     3181 tcgattttaa gactctgaat gtttcaatgc cgaaatggct tggcgttact acgtctgaga
     3241 aataatcaat taatttatct agatgttttt tatctattcg tccatttttt atccaacctg
     3301 aaacgcttgg ctgtttcacc ccaaaatgat cggcaacctc cttttgactg acattttttc
     3361 tcttaatcgc ttctgatatt gcttgtccta actgttcgcc tgaaaacatt ttgatctccg
     3421 aattaattgc gcataagcat tggtaatcga taataaaagg cataacctat aaaaggcaat
     3481 agttgtattt aatataagta ttagctataa tgaagctatt taattgagta actggctatg
     3541 agtatccaaa aagcagttga ttattttggt aatgaatccc gacttgcacg ggcgatcgga
     3601 gttaaacaac cgacggtgtg ggcttggaat aaaaaaggaa cgccgccccc gatcattcgg
     3661 tgcgtgcaga ttgaaaaatt aaccggaggc gcagtgaatc gaaaagactt acgtcctgat
     3721 gactggcatc taatctggcc ggaattggca ggcgaccaac ccaaataaaa agcccgtcgg
     3781 gtcagatgtt aattgatata accgaattga agcggaagtc atccgcaatt taccggaaag
     3841 gaaaaaaatg aagaagcagg acagaaac
//
LOCUS       DAWTQS010000106         3814 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.106, whole genome shotgun sequence.
ACCESSION   DAWTQS010000106 DAWTQS010000000
VERSION     DAWTQS010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.106"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(<1..169)
                     /locus_tag="WNA25_002028"
     CDS             complement(<1..169)
                     /locus_tag="WNA25_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFA9824695.1"
                     /translation="MRNTVGLDISKLTFDATAIVGNAEYSAKFDNDSKGLDQFSDRLK
                     SLGCQNLHICME"
     gene            216..638
                     /locus_tag="WNA25_002029"
                     /pseudo
     CDS             216..638
                     /locus_tag="WNA25_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201279.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
     gene            complement(693..1148)
                     /locus_tag="WNA25_002030"
     CDS             complement(693..1148)
                     /locus_tag="WNA25_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-helix bundle copper-binding protein"
                     /protein_id="HFA9824696.1"
                     /translation="MNRRQFLGSAAAVSLASAASFARAHGHADYHHHHDMQPAAASAY
                     TAVRQTAAHCLDAGQVCLTHCLSLLTQGDTSMSDCAVAVRQMLALCGAVHDLAAQNSP
                     LTRDAAKVCLEACKQCAKACKEHSAHHAECKACYESCLDCIKECEKLAA"
     gene            complement(1404..2774)
                     /gene="ffh"
                     /locus_tag="WNA25_002031"
     CDS             complement(1404..2774)
                     /gene="ffh"
                     /locus_tag="WNA25_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFA9824697.1"
                     /translation="MLDNLTGRFSNVFKNIRGQAKLTEDNIKEALREVRLALLEADVA
                     LPVVKEFVNNVKEKALGQEVAGSLTPDQAFIGVVNQALVELMGKENKTLDLSVSPPAI
                     ILMAGLQGAGKTTTVGKLARLLKNDQKKKVLVVSADVYRPAAIEQLRLLAEQVGVDFF
                     PSDTNQKPVEIATAAVDYAKKHFYDVLMVDTAGRLAIDEEMMNEIKALHAVVNPVETL
                     FVIDAMLGQDAVNTAQAFNEALPLNGVVLTKMDGDSRGGAALSVRHVTGKPIKFIGVG
                     EKINGLEPFHPDRLASRILGMGDVLTLIEDVQKGIDEEAAAKMAKKLQKGKGFDLNDF
                     KEQIQQMRNMGGLENLMSKMPGELGQISKQIPEGTAEKAMGKVEAIINSMTPKERANP
                     ALLKAGRKRRIAMGAGTTVQEVNKLLKQFEQMQQMMKMFSGNGLGKLMRLAKGMKGIK
                     GMFPGL"
     gene            2996..3802
                     /locus_tag="WNA25_002032"
     CDS             2996..3802
                     /locus_tag="WNA25_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFA9824698.1"
                     /translation="MPTVFIFLTAVYAGLGAFAWHCQQQGRGRDYPWKTELPVLGAAL
                     TVHGAALLMPVIQDKIIIMGFGYSGSLIVWMMLFIYFAGSFFYPLRGVQLLLYPCAAL
                     MLLSGLVFPGKFSGYEITDFPFMLHIGTSLLAYGLFGIATLLSVLSLLLHRSLHRRNF
                     SKLAGFLPSLLSLEKLMFQAMWAGFILLTYSVVSGTFFAEAVFGKPMTFTHKTVFGIL
                     SWLIYGGLLLKHSMTAWRGKKAAVWTIIGFVSLMIAYMGSKFVLEIILKR"
BASE COUNT          844 a          946 c         1010 g         1014 t
ORIGIN      
        1 cctccatgca gatatgcaga ttctgacatc ccaagctttt caaccggtcc gaaaactgat
       61 ctaaaccttt tgaatcgttg tcaaactttg ccgaatattc ggcattgccg acaatggccg
      121 ttgcgtcaaa agtcaacttg gatatgtcca atcctacggt gttacgcatg ggattaccct
      181 tatatattgc gtccctaaga aggggcgcaa tatataaggt gtggcaattt gtcgaagacg
      241 cgctgcgtgc cgtcgtgcct gccgacagtt ttgaaccgac cgcgcaaaaa ttgaagctgt
      301 ttaaggcggg cgcggcaacc attttgtttt atgaagatca aaatgtcgtc aaaggtttgc
      361 aggagcagtt ccctgcttat gccgccaact ttcccgtttg ggcggaccag gcgaacgcta
      421 tggtacagta tgccgtctgg acgacacttg ccgcggtcgg tgcaggtgca aatctgcaac
      481 attacaaccc cttgcccgat gcggcgattg ctaaagcgtg gaatattccc gaaaactggc
      541 tgttgcgcgc gcaaatggtt atcggtggta ttgaaggggc ggcaggtgaa aaggtctttg
      601 aacccgttgc ggaacgtttg aaagtgttcg gcgcataatt tcgcggtcaa aaaaatgccg
      661 tctgaacccc gttcagacgg catttttcag tatcaggcgg cgagtttttc gcattctttg
      721 atacagtcga ggcaggactc gtaacaggct ttgcattccg catggtgggc ggagtgttct
      781 ttacaggctt tggcacactg tttgcacgct tcgaggcaca cttttgccgc gtcgcgtgtc
      841 agaggggaat tttgtgcggc aaggtcgtgc accgcgccgc ataaggcaag catctggcgc
      901 acggcaaccg cacagtcgga catagacgtg tcgccctgag tgagcaggga caggcagtgg
      961 gtcaggcaaa cctgtccggc atccagacag tgtgcggcag tttggcggac ggcggtgtag
     1021 gcggatgcgg cggcaggctg catatcgtga tgatggtggt agtcggcgtg tccgtgcgca
     1081 cgcgcgaaag aggcggcgga agccaaagag acggcagcgg cgctgcccaa aaattgacga
     1141 cggttcatga ttttttcctt tatgtttcaa catgcaggtc cggtataagg tacacactta
     1201 ccatactgcc ctggccggat cggtacgatt ttctgaataa ttgcttgatt ttgcaggtgt
     1261 gaaattgtcg cacgagcagg catgttttag ctattcggaa attcgaggaa gggtgcatca
     1321 acctgttttg aaatgatgcg cagacatggt aacagacaaa aagccgtctg aaaatcgttt
     1381 tagacggctt ttgttttcaa acattaaaga ccgggaaaca ttccctttat tcccttcatg
     1441 cctttggcca agcgcatcag tttgcccaaa ccgttgccgc tgaacatctt catcatctgc
     1501 tgcatttgtt caaactgttt gagcaatttg ttcacttcct gcacggtcgt gcccgcgccc
     1561 atcgcaatac ggcgtttgcg gccggctttg agcagggcag ggttggcgcg ttctttaggg
     1621 gtcatcgagt tgatgatggc ttctactttg cccatcgctt tttcagccgt accttcgggg
     1681 atttgtttcg atatttgacc cagttcgccc ggcattttcg acatcaggtt ttccaaaccg
     1741 cccatattgc gcatttgctg gatttgttct ttaaaatcat tgaggtcaaa gcctttgcct
     1801 ttttgcagct ttttcgccat tttggcggcg gcttcttcat caataccttt ttgaacgtct
     1861 tcaatcaggg tcaatacgtc gcccatgccg aggatacggc tggcaagacg gtcggggtgg
     1921 aaaggttcga ggccgttgat tttctcgccg acgccgataa atttaatcgg tttgccggtt
     1981 acatggcgta cggacaatgc cgcaccgccg cgcgagtcgc cgtccatctt ggtcaatacg
     2041 acgccgttca gcggcagggc ttcattaaat gcctgagcgg tatttaccgc atcctgaccc
     2101 agcatggcat cgataacgaa caaagtttca accgggttga ccaccgcatg aagcgctttg
     2161 atttcgttca tcatctcttc atcgattgcc aaacggccgg cggtatcgac catcaataca
     2221 tcgtaaaaat gttttttggc gtaatcaacg gcggcagttg caatctcaac cggtttttgg
     2281 ttggtatcgg atgggaaaaa atctacgccg acctgttcgg ccaacaggcg cagctgttca
     2341 atcgcggcag ggcggtaaac gtcggcggat accaccaaaa cttttttctt ctgatcgttt
     2401 ttcagcaggc gggcgagttt gccgacggtt gttgtcttac cggcaccctg caaaccggcc
     2461 atcaaaatga tggcaggcgg tgaaaccgat aaatccagtg ttttgttttc cttgcccatc
     2521 agttcgacta aggcttggtt gaccacgccg ataaaggctt ggtcaggcgt caggctgccc
     2581 gccacttcct gaccgagggc cttttctttg acgttgttga cgaactcttt gacgacaggc
     2641 agggcgacat ccgcctcaag cagggcgagt cggacttcgc gcaaggcttc tttaatattg
     2701 tcttcggtca gtttggcctg cccccggatg tttttgaaga cattgctgaa gcggccggtt
     2761 aaattgtcta acatacttgg tccttggtct gaagtaagaa tagcttgccc catcaggggc
     2821 attctttgtt aaaataaaat caaaataatt tgatgcggct tgtgtgccgg acagcatatc
     2881 ggcaaatccg tcaaggcttg cccgaaacgg ggattttaca attccaacgt taaaagttcc
     2941 aatatttcat aagcggccgc atacggcgca acagtataga tagagaaagt ccaccatgcc
     3001 gacagttttc atctttttga cggcggttta cgcaggattg ggtgcatttg catggcactg
     3061 ccaacagcag gggcgcggcc gggattaccc gtggaagacg gaattgccgg ttttgggcgc
     3121 ggcattgacc gtccacggcg cggcactgct gatgccggtc attcaagaca aaatcatcat
     3181 tatgggcttc gggtattccg gcagcctgat tgtttggatg atgctgttta tttattttgc
     3241 cggcagcttt ttttatccgc tgcgcggagt gcagttgctg ctgtatcctt gcgccgcact
     3301 gatgctgctg tcaggtttgg ttttcccggg aaaattctcg ggatatgaaa ttaccgactt
     3361 tccctttatg ctgcatatcg gcacatcact gcttgcatac gggctgttcg gcatcgcaac
     3421 attattgtcc gttttgagct tgctgctgca ccggagcctg caccgcagaa acttctccaa
     3481 gctcgcagga ttcctgccgt cgctgctcag tttggaaaaa ctcatgttcc aggccatgtg
     3541 ggcaggtttc atcctgctga cctattccgt cgtcagcggc acattctttg ccgaagccgt
     3601 attcggcaaa cccatgacat ttacccataa aaccgtattc ggcatattgt catggctgat
     3661 ttacggagga ctgctgctca aacacagcat gaccgcatgg cgcggcaaaa aagccgccgt
     3721 gtggaccatc atcggatttg tcagccttat gattgcctat atgggcagca agttcgtatt
     3781 ggaaatcatt ctgaaaagat aagaagagcc aaca
//
LOCUS       DAWTQS010000107         3736 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.107, whole genome shotgun sequence.
ACCESSION   DAWTQS010000107 DAWTQS010000000
VERSION     DAWTQS010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.107"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            1..100
                     /locus_tag="WNA25_002033"
                     /pseudo
     CDS             1..100
                     /locus_tag="WNA25_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            551..745
                     /locus_tag="WNA25_002034"
     CDS             551..745
                     /locus_tag="WNA25_002034"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter"
                     /protein_id="HFA9824699.1"
                     /translation="MINLKPAIEAGYTGRGVEVGIVDTGESVGSISFPELYGRKEHGY
                     NENYKNKLQKLYGVYAEGSA"
     gene            726..3409
                     /locus_tag="WNA25_002035"
                     /pseudo
     CDS             726..3409
                     /locus_tag="WNA25_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261022.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
BASE COUNT         1003 a         1013 c         1005 g          715 t
ORIGIN      
        1 ttgagccgta tttaaaggag tgcgaacggc gttttaacaa cagtgagata aaagttcttg
       61 ttccatttta aaacaattag taaaatcgag tttatcctag ttgtccagga cagatataat
      121 tcaaaatata aaaatgggtt acatctggac attacgaaat ttttattcct tcgcctgaat
      181 tctattgtca gattcaaaga gacctcatca tgcgaaccac ctcaaccttc cctacaaaaa
      241 ctttcaaacc ggctgccatg gcgttagctg ttgcaacaac actttctgcc tgcttaggcg
      301 gcggcggagg cggcacttct gctcccgact ttaatgcagg cggcaccggt atcggcagca
      361 acagcagggc aacgatagcg gaatcagcag cagtatctta cgccggtata aaaaacgaaa
      421 tgtgcaaaga cagaagcatg ctctgtgccg gtcgggatga cgttgcggtt acagacaggg
      481 atgccaaaat caaagccccc gaatctgcat accagagact tttcaaaccc aaatgaccaa
      541 tattaagaat atgatcaacc tcaaacctgc aattgaagca ggctatacag gacgcggggt
      601 agaggtaggt atcgtcgata caggcgaatc cgtcggcagc atatcctttc ccgaactgta
      661 tggcagaaaa gaacacggct ataacgaaaa ttacaaaaac aaattacaaa aactatacgg
      721 cgtatatgcg gaaggaagcg cctgaagaca gaggcggtaa agacattgaa gcttctttgg
      781 acaatgaggc cgttatagag actgaagcaa agccgacgga tatccgccat gtaaaagaaa
      841 tcggacacat cgatgtggtc tcccatatta ttggcgggcg ttccgtggac ggcagacctg
      901 caggcggtat tgcgcccgat gcgacgctac acataatgaa tacgcatgat ggaaccaaga
      961 acgagaaata atgtctgcag ccatccgcaa tgcatgggtc aagctgggcg aacgtggcgt
     1021 gcgcatcgtc aataacagtt ttggaacaac atcgagggca ggcactgccg accatttcca
     1081 aatagccaat tcggaggagc aataccgcca aacgttgctc gactattccg gcggtgataa
     1141 aacagatgag ggtatccgcc tgatgcaaca gagcgattag ggcaacctgt cctaccacat
     1201 ccgtaataaa aacatccttt tcatcttttc gacaggcaat gacacacaag ctcagcccaa
     1261 cacactgacc ctattgccat tttatgaaaa gacgctcaaa aaggcattat cacagtcgca
     1321 ggcgtagacc gcagtggaga aaagttcaat ggctccaacc attgcggaat tactgccatg
     1381 tggcgcctgt cggcatccta tgaagcaacc gtccatttca cccgtacaag accgattcaa
     1441 gttgccggaa catccttttc cgcacccatc gtaaccggca cggcggctct gctgcagaga
     1501 tacccgtgga tgagcaacga caacctgcgt accacgctgc tgacgacggc tcaggacatc
     1561 ggtgcagtcg gagtggacag caagttcggc tgggggctgc tggatgcggg taaggccatg
     1621 aacggacccg cgtcctttcc gttcggcgac tttaccgccg atacgaaagg tacatccgat
     1681 attgcctact ccttccgtaa cgacatttca ggcacgggcg gtctgatcaa aaaaggcggc
     1741 agccaactgc aactgcacgg caacaacacc tatacgggca aaaccgttat cgaaggcggt
     1801 tctctggtgt tatcggatat gcacgtcaaa accaaaggtg cactgattta taacggggcg
     1861 gcatccggcg gcagcctgaa cagccacggc attgtctatc tggcagatac cgaccaatcc
     1921 ggcgcaaacg aaaccgtaca catcaaaagg cggtctgcag ctgggcgaca aaggtacgct
     1981 gtacacacgt ttgggcaaac tgctgaaagt ggacggtacg gcgattaccg acggcaagct
     2041 gtacatgtcg gcacgcggca agggggcagg ctatctcaac agtaccggac gacgtgttcc
     2101 cttcctgagt gccgccaaaa tcgggcagga ttattctttc ttcaaaaata tcgaaaccga
     2161 cggcggtctg ctggcttccc tcgacagcgt cgaaaaaaca gcgggcagtg aaggcgacac
     2221 gccgtcctat tatgtccgtc gcggcaatgc ggcacggact gcttcggcag cggcacattc
     2281 cgcgcccgcc ggtctgaaac acgccgtaga acagggcggc agcaatctgg aaaacctgat
     2341 ggtcgagctg gatgcctccg aatcatccgc aacacccgag acggttgaaa ctgcggtcgc
     2401 cgaccgcaca gatatgccgg gcatccgcct acggcgcaca actttccgcg cagcggcagc
     2461 cgtacagcat gcgaataccg ccgacggcgt acgcatcttc aacagtctcg ccgctaccgt
     2521 ctatgccgac agtgccgccg cccatgccga tatgcaggga cgccgcctga aagccgtatc
     2581 ggacgggttg gaccacaacg gtacgggtct gcgcgtcatc gcgcaaaccc aacaggacgg
     2641 tggaacgtgg gaacagggcg gtgtcgaagg caaaatgcgc ggcagtaccc aaactatcgg
     2701 cattgccgcg aaaaccggcg aaaatacgac agcagccgcc acactgggca taggacgcag
     2761 cacatggagc gaaaacagtg caaatgcaaa aaccgacagc attagtctgt ttgcaggcat
     2821 acggcacgat gtgggcgata tcggctatct caaaggcctg ttctcctacg gacgctacaa
     2881 aaacagcatc agccgcagca ccggtgcgga tgaatatgcg gaaggcagcg tcaacggcac
     2941 gctgatgcag ctgggcgcac tgggtggtgt caacgttccg tttgccgcaa cgggagattt
     3001 gacggttgaa ggcggtctgc gccacgacct gctcaaacag gatgcattcg ccgaaaaagg
     3061 cagtgctttg ggctggagcg gcaacagcct cactgaaggc acactggtcg gactcgcggg
     3121 tctgaagctg tcgcaaccct tgagcgataa agccgtcctg tctgcgacgg cgggcgtgga
     3181 acgcgacctg aacggacgcg actacgcggt aacgggcggc tttaccggcg cggctgcagc
     3241 aaccggcaag acgggtgcac gcaatatgcc gcacacccgc cgggttgccg gtctgggggt
     3301 ggatgtcgaa ttcggcaacg gctggaacgg cttggcacgt tacagctaca ccggttccaa
     3361 acagtacggc aaccacagcg gacaaatcgg cgtaggctac cggttctgac ggacagaaaa
     3421 cagacagccg caaagatcac ggtctttgcg gctgtttctt atgaaaagaa aaccctattc
     3481 caattgcctg cttctattgt ttcaagactt cttccaaaga ttcggcatca atcagacgta
     3541 tagcggatta acaaaaatca ggacaaggcg gcgggccgca ggcagtacgg atggtacgga
     3601 accgatccgc ccggtgcttc agcaccttag ggaaccgttc cctttgagcc ggggcggggc
     3661 aacccgtacc ggtttttgtt catccgccat attgtgttga aacaccgccc ggaacccgat
     3721 ataatccgcc cttcaa
//
LOCUS       DAWTQS010000108         3572 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.108, whole genome shotgun sequence.
ACCESSION   DAWTQS010000108 DAWTQS010000000
VERSION     DAWTQS010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.108"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(1..101)
                     /locus_tag="WNA25_002036"
                     /pseudo
     CDS             complement(1..101)
                     /locus_tag="WNA25_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235291.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(119..463)
                     /locus_tag="WNA25_002037"
     CDS             complement(119..463)
                     /locus_tag="WNA25_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824700.1"
                     /translation="MNLENYENILIKLLFHHNNLVNEYSYFIENKNLFKIVSRTKTSK
                     GFFFTIEKPLNFLSKKTYFEFNFKYLHSGKERFGSFMCWINTNLMEFEGVFFNDPLPD
                     NMIINNFFEIND"
     gene            complement(862..1263)
                     /locus_tag="WNA25_002038"
     CDS             complement(862..1263)
                     /locus_tag="WNA25_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824701.1"
                     /translation="MKLKSLDFPTGYFYFDNAAINFDKVEVIAVGYRNTGKTIKISIE
                     DVIHFRVVDESYFIDTFMDLISEDADRALLHENGGQSFFELLDERYAAWISKESYFPL
                     NREFFKYYIFMFEQTFIEIIGSSATYSIIES"
     gene            complement(1267..2016)
                     /locus_tag="WNA25_002039"
                     /pseudo
     CDS             complement(1267..2016)
                     /locus_tag="WNA25_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713004.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(2247..2606)
                     /locus_tag="WNA25_002040"
     CDS             complement(2247..2606)
                     /locus_tag="WNA25_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824702.1"
                     /translation="MMTEPHGYPSNNLPVKIINDIIYATQLVEDLVLGKIKIVDFLKS
                     YNNFYYWLGFDELPQSEKIKFLNYLNILSIHKEIQDKTVNRVYTDCFDIDKLHSLDRI
                     TTNECINGIKKSIKKTN"
     gene            complement(2627..2833)
                     /locus_tag="WNA25_002041"
     CDS             complement(2627..2833)
                     /locus_tag="WNA25_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824703.1"
                     /translation="MMENSKKTWEIDGEIWLHCPVCGTEVMDYDICDVCQWQNTGETN
                     IDGGPNEMTLAEAKEAYAKGLPIR"
     gene            complement(2799..>3572)
                     /gene="mafB"
                     /locus_tag="WNA25_002042"
     CDS             complement(2799..>3572)
                     /gene="mafB"
                     /locus_tag="WNA25_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HFA9824704.1"
                     /translation="AVNPVTYAAAQQTLQGIHDLGNVSPAAQLAAASLLQDSAFAVKD
                     SINSAKQWADAHPNITATAQTALAVAEAAATVWRGKKVKLNPTKWDWVKNTGYKKPAA
                     RPMQTVDGEMAGKNKPPKPSTQQHSTHSDNNIGLPAPYVKPDTSISPTGTIQDRIRWT
                     KSKFPTEKSLNGHFKAHGKEFGDITIEDYQKMASDLLSKQTSDKILGYQTEHRRVRYD
                     INNNIYVLANPKTFKIKTMFKPDLGKEYYDGEFKKDMGN"
BASE COUNT         1009 a          712 c          603 g         1248 t
ORIGIN      
        1 gccccaactc ccaatccttg aaaacccgtt aaaaaaggat taaccgcacc ttggacgatg
       61 ccgcgaatat catccatacg gttaccccac caatttttat tggtttattg gtatatcgtt
      121 aatcatttat ttcaaaaaag ttatttatta tcatattatc agggagcggg tcgttaaaaa
      181 aaactccctc aaattccatt aaattagtat ttatccagca cataaacgaa ccaaagcgtt
      241 ctttccctga gtgtaaatat ttaaaattaa actcaaaata agtttttttg cttagaaaat
      301 ttaatggttt ttctatagtg aaaaaaaagc ccttactcgt tttagttcga gatactattt
      361 taaataagtt tttattctca ataaaataag aatattcatt tactaagttg ttatgatgaa
      421 aaagtaattt tattaaaatg ttttcataat tttctagatt catttctagc tcaatttaaa
      481 atgatttcat tgttgccgta ccaatgcgaa atttaccatc aacaaaattt cccttcactg
      541 atacaggtac cccatcgcag agatgtaggt cggatatttg tatccgacaa aaatatttga
      601 cgcgtttatc gtttccgaaa tgccgctgtt agaaaatgtc ggattcgaga atccaaccta
      661 cggctatatc cgtaaagtct gaccattgtt caatgcggaa ggcgcgaata ttgcggacgg
      721 aatgtagaaa ccaatatact taattgccat gccattctca tcaaaatacc tgtgctgttt
      781 aaagtgcgtg gttcattaca cgagtaattt tattttcagg ctgcctgaaa gaaagatagg
      841 caacttatta ctcatcttac gctagctctc aataattgaa tacgttgcac tagaaccaat
      901 tatttctatg aatgtttgct caaacataaa aatatagtat ttaaagaatt ctctattcaa
      961 aggaaaataa ctttctttcg atatccatgc cgcataacgc tcatcaagaa gttcgaaaaa
     1021 agattgacca ccattttcat gaagcaaagc tctatctgca tcttccgaaa ttaaatccat
     1081 aaaagtatct ataaaatacg attcatcaac aaccctaaaa tgaataacat cctcaataga
     1141 aatttttatg gttttacccg tatttctata accaactgct ataacttcta ctttatcaaa
     1201 gtttattgct gcattatcaa aatagaaata gccagttggg aaatctaagc tttttaattt
     1261 cataatttat cgtgtatttc catatctgat ttttattttt cggttattac taattgttat
     1321 ttcaagtgtt ggttctccac cactgctatc attacgaaca ttaaccgcac gcccatcagg
     1381 taaattgccc gttatcgttc cattaggacg tgtttgtaca tttcgaacat tcagactatt
     1441 aaaatcattt agtgcgtcat tgtaatcccc cactttatca tactgaacag cgggaccttt
     1501 tgttttactc ctttgagtag tattgctcaa aatgtctttg atttgtaaat cactgcgcac
     1561 cttaccggct tctatacgag ctgacacact actcgggcta taggggtcgc tatcaatcgc
     1621 aggattacca ccgctggttt tttgtccgct agtctgtttc ccaacctgct gtactgtatt
     1681 gccaatttca tctaccgtac ctaacgcctt agtctgcata tggcgggcag taggtttttt
     1741 ataaccgtta tttttaaccc aatcccattt ggccgggtta agttctactt ttttaccgcc
     1801 ccaaaccgta cctgcagcct ctgctacggc aagggcagtt tgggctgttg ctgttatatt
     1861 cggatgggca tcagcccatt gtctggcgga attgatgccg tcttttaccg caaaagcact
     1921 gtcctgtaat aggctcgcgg cagcaagttg tgcttccgga cttaaatttc ctaattcatt
     1981 aatatcttgt aaagtttgct gcgcagagat gtaggtcgga tatttgtatc cgacaaaaat
     2041 atttgacgca tctatcgttt ccgaaatgcc gctgttggaa aatgtcggat tcgagaatcc
     2101 gacctacggc tgctgactat tttcttgttt atataattct acaactgcaa actactatca
     2161 aatcttttta tctctggctc tacactcagc aagaatattt ttattttaca aaacataggc
     2221 tagttatttg agtatattgt caaattctaa tttgtttttt tgatagattt ttttatacca
     2281 ttaatacatt cattagttgt aattctgtca agcgaatgta atttatcaat atcaaaacaa
     2341 tcggtataaa ccctattcac agttttatct tgtatttcct tatgaatact taatatattg
     2401 agatagttta gaaattttat tttctcagat tgaggtaact catcaaaacc aagccaataa
     2461 taaaaattgt tatatgattt taaaaaatca acaattttta tttttcctaa aaccaaatct
     2521 tcgactaact gggttgcata aataatatca tttatgattt taacaggtaa attatttgat
     2581 gggtaaccat ggggttccgt catcattggt ttctctaggt gcttatttat ctgattggta
     2641 agccttttgc gtaagcttct ttcgcctccg caagtgtcat ttcattagga ccaccatcta
     2701 tattagtttc tcctgtattt tgccactgac aaacgtcaca gatatcatag tccataactt
     2761 cagttccgca aacaggacaa tgtagccata tttctccgtc aatttcccat gtcttttttg
     2821 aattctccat cataatactc ctttcctaag tctggtttaa acattgtttt gattttgaat
     2881 gtttttggat tggccaaaac atagatattg ttattgatat catagcgcac tcgtctatgt
     2941 tccgtctgat aacctaatat cttgtccgat gtctgttttg ataacaaatc agacgccatt
     3001 ttttggtagt cttcaatggt tatatcgcca aattcttttc catgagcttt gaaatgtcca
     3061 tttaaagatt tctcagtagg aaacttggat tttgtccatc tgatgcggtc ttgaattgtt
     3121 cctgtcggag aaatagatgt atcaggttta acatatgggg caggtaagcc gatattgtta
     3181 tcagagtgtg tagagtgttg ctgcgtactt ggtttcggtg gcttattttt cccggccatt
     3241 tccccgtcta cagtctgcat aggtcgggca gcaggttttt tatagccggt atttttaacc
     3301 caatcccatt tggtcgggtt aagttttact tttttacctc tccaaaccgt agctgcggcc
     3361 tctgctacgg caagggcagt ttgggcagta gctgtaatgt tcggatgggc atcagcccat
     3421 tgcttggcgg aattgatact gtccttaacc gcaaaagcac tgtcttgtaa taggctcgcg
     3481 gccgcaagtt gtgctgcggg acttacattt cctaaatcgt gaataccctg taaagtttgc
     3541 tgtgctgccg cataggttac cgggtttacc gc
//
LOCUS       DAWTQS010000109         3545 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.109, whole genome shotgun sequence.
ACCESSION   DAWTQS010000109 DAWTQS010000000
VERSION     DAWTQS010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.109"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            <1..2072
                     /locus_tag="WNA25_002043"
     CDS             <1..2072
                     /locus_tag="WNA25_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFA9824705.1"
                     /translation="PEEQAELEKAVAYAFRAHDGQTRKSGDPYITHPIAVATQLALWH
                     MDIQGLCAGVMHDVLEDTGVTKGEMAAVFGNTVAEMVDGLSKLEKLKFEDHAEHQAES
                     FRKLILAMTKDVRVIVVKLADRLHNMRTLGSMRPDKRRRIARETLEIYAQIANRIGLN
                     NAYRELQDLSFQNLHPNRYETLKKAMDKSRKNRQDVVGKVLRAFGQRLVGANIEAKIK
                     GREKNLYGIHQKMMAKKLRFAEVMDIYGFRVIVNSIPACYAALGTLHTLYQPKPGRFK
                     DYIAIPKSNGYQSLHTILVGPYGLPIEVQIRTREMDAVAEGGIVGHWSYKSDSKTVDQ
                     AVLHTNQWLKNILDLQASSANAIEFLEHVKVDLFPNEIYILTPKGKILTLPKGATPVD
                     FAYAVHTDIGHKTVAARVNNVMMPLRTKLKTGDSVEIITSEHAKPNPAWLNFAVSGRA
                     RSAIRQYIKNLNRHDAVVLGESLLQKALSSLLPKDVLLSDGIKEKYLADLNDKQTSFE
                     EVLYNVGMGHTLPVYVAMHIAELAGEHFGSEVRLSSIKVDGQESGHIHFAECCHPVPG
                     DSIRLLLVKGKGMIIHRDTCPTLLKSDPEQQLDADWENMNGQNYRVGLQVQSEDSHGL
                     LALMAQAISDSGADIESVETPSKSQSGTEGFVEFKFLLKVKNLDQLNQIIQNLHSIPY
                     IRKVIRS"
     gene            complement(2139..3323)
                     /gene="coaBC"
                     /locus_tag="WNA25_002044"
     CDS             complement(2139..3323)
                     /gene="coaBC"
                     /locus_tag="WNA25_002044"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFA9824706.1"
                     /translation="MGKHILLGVTGSIAAYKSCELVRLLKKQGHSVTVVMSRSATEFV
                     SPLTFQALSGNPVLTDTHGGNGSNGMEHINLTRNADVFLIAPASMNTVAKICNGVADN
                     LLTNLAAARKCPLAIAPAMNVEMWLNPANQRNIAQLVSDGITVYMPGLGEQACRENGM
                     GRMPEPAELLDLLPDLWTPKILRDKKILITAGATFEAIDPVRGITNTSSGKMGVALAR
                     ACRAAGAEISLIHGQLQTTLPFGISDTVQAVSAEDMHRAVHRLIEKQDAFISVAAVSD
                     YKVKNRSTQKFKKDKNAKPLSIELDENPDTLASIASLPNPPFCIGFAAETENVMAYAR
                     EKRIKKKIPVIVANDVSIAMGKTTNQIVIIDDDAELSFPETSKDEAAMRIVERLAVYL
                     NK"
BASE COUNT          930 a          876 c          881 g          858 t
ORIGIN      
        1 agcccgagga acaggcggag cttgaaaaag ctgtcgccta tgcgtttcgc gcccacgacg
       61 ggcaaacccg caaaagcggg gatccctaca tcacgcatcc gattgccgtt gcgacgcagc
      121 tcgccctttg gcatatggac atacagggtc tttgtgcagg cgtgatgcac gacgtattgg
      181 aagatacggg cgtgacaaaa ggggaaatgg cggcggtgtt cggcaatact gttgccgaga
      241 tggtggacgg gctgtccaaa ctcgaaaaac tcaaatttga agatcatgcg gaacatcagg
      301 cggagagttt ccgcaaactg attttggcga tgaccaaaga tgtgcgcgtc atcgttgtca
      361 aacttgccga ccgcctgcac aatatgcgga cgctcggttc gatgcgcccg gacaaacgcc
      421 gccggattgc aagggaaacc cttgaaatct acgcacagat tgccaaccgc ataggtctga
      481 ataatgcgta tcgggagctt caggatttat cgttccaaaa cctgcatccc aaccgctacg
      541 agactttaaa aaaagcgatg gacaagagcc ggaagaacag gcaggacgtt gtcggcaaag
      601 ttttgcgcgc gttcggccag cggctggtag gcgcgaatat agaggccaaa atcaaaggca
      661 gggaaaaaaa cctgtacggc atccatcaga aaatgatggc gaaaaagctg cgctttgccg
      721 aggttatgga tatttacggt ttccgcgtca ttgtcaacag cattcccgcc tgttatgccg
      781 cactcggcac attgcacacc ctctatcagc ccaagcccgg acggttcaaa gactatatcg
      841 ccattccgaa aagcaacggt tatcaaagtc tgcatacgat tttggtcggc ccctacggct
      901 tgccgattga agttcagata cgcacgcggg aaatggatgc cgtggcggaa ggcggtattg
      961 tcggccattg gagctataaa tcagattcca aaacggtcga tcaggcagtg ctgcacacca
     1021 atcaatggtt gaaaaacatt ttagacttgc aggcaagcag cgccaatgcc attgagtttc
     1081 tcgaacacgt caaagtcgat ttgtttccga acgaaatcta catccttact ccgaaaggaa
     1141 aaatcctaac tttgcccaaa ggggcaacgc ctgtcgattt tgcttatgcg gtgcataccg
     1201 acattgggca taaaacagtt gccgcgcgtg tcaataatgt gatgatgccg ctgcgtacga
     1261 agctcaaaac cggtgattct gttgaaatta tcacatccga acacgccaaa cccaatcccg
     1321 cgtggttgaa tttcgccgtg tccggcaggg cgcgcagtgc catacgccaa tatattaaaa
     1381 atcttaaccg gcacgatgcg gtcgttttgg gagagagcct cttacaaaaa gccttgtcca
     1441 gtttgctgcc caaagatgtc ctgctttcag acggcatcaa agaaaaatac cttgccgatc
     1501 tcaacgacaa acagacttca tttgaggaag tgctgtacaa cgtcggtatg gggcataccc
     1561 tgcctgttta tgtcgccatg catatcgccg aattggcagg ggagcatttc ggcagcgagg
     1621 tcaggctcag ttccattaaa gttgatggtc aggaaagcgg gcatattcat tttgcagagt
     1681 gctgccaccc tgttcccggc gattccatcc gtttgctgtt ggttaaggga aaaggcatga
     1741 ttatccatag ggatacctgc ccgacgttgt tgaagtccga tcccgaacag cagctggatg
     1801 cagactggga aaatatgaac gggcagaact accgtgtcgg gcttcaagtc caatcggaag
     1861 acagccacgg cctgttggca ttaatggcgc aagcgatttc cgattccggt gcggacatcg
     1921 agtcggtcga aacaccgtct aaatcccagt cgggaacgga aggttttgtc gaattcaaat
     1981 tcttattgaa agtcaagaat ttggatcagt tgaatcagat tattcaaaat ctgcacagca
     2041 tcccatatat ccgcaaagtc atcagaagtt gaagcaggta aaacgggttg cctgttaatc
     2101 aaaaggcaac ccgttttatg gtttatccgt tcaattgctt atttgttcaa atatacggca
     2161 agcctttcaa caatccgcat tgccgcttca tctttacttg tttcggggaa agacaactcc
     2221 gcatcatcat ctatgataac aatttggttg gttgtcttgc ccatcgcgat tgaaacatca
     2281 ttggcaacta tcaccggtat ttttttctta atacgttttt cccgcgcata tgccattaca
     2341 ttctccgttt cagcggcaaa accgatgcag aacggcgggt tcggcaatga ggcaatagaa
     2401 gccaaagtat cggggttctc atccaattcg atggataacg gtttggcatt tttatctttt
     2461 ttgaattttt gcgtactcct gtttttaacc ttatagtctg agacggcggc aacagaaata
     2521 aaagcatctt gtttttcaat caaacgatgt actgcgcgat gcatatcttc ggcactgacg
     2581 gcttgaaccg tatcggatat gccgaaaggc agcgtggttt gaagctgtcc gtgaatcagg
     2641 ctgatttccg caccggcggc acggcacgcc cgcgccaaag ctacgcccat ttttccgctg
     2701 gaggtatttg tgatgcccct gacagggtca atggcttcaa atgtcgcacc tgcggtaatc
     2761 aagattttct tgtcccttaa aattttcggt gtccacaagt ctggaagcaa atccagcaat
     2821 tcggcaggtt ccggcatcct tcccatacca ttttctctgc aagcctgttc gcccaagccc
     2881 ggcatataga cagtaatgcc gtctgaaacc agttgtgcga tattccgttg gttggcagga
     2941 ttcagccaca tttccacatt catcgcggga gcgatggcaa gagggcattt ccgtgcggct
     3001 gccagattgg tcagtaggtt atctgccacg ccgttacaga tttttgccac ggtattcata
     3061 cttgccggcg caatcagaaa aacatccgca ttccgggtca ggttgatatg ttccataccg
     3121 tttgaaccgt tgccgccgtg cgtgtcggtc agaacgggat tgccgctcaa agcctgaaaa
     3181 gtcagcggag aaacaaattc agttgccgag cggctcataa ccaccgtaac cgaatgcccc
     3241 tgttttttca gcagtcgcac caactcgcaa gacttatacg ccgcaatact gcccgttaca
     3301 cctaaaagaa tatgtttgcc cattttgaca atttctaatt acgaagaaaa aacagcagcc
     3361 attctataac aaagtacgga tacattagtt accgtttgga tacgaaatgc cgtctgaagc
     3421 cccgagtttc aggcggcata ttcacaaagg cgcatcagcc ggaggagaag agggggttgt
     3481 tggaggcggc gcagcgtttg gcggagataa aaaaccttat ccgacagcga catgacgaat
     3541 ttccc
//
LOCUS       DAWTQS010000110         3395 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.110, whole genome shotgun sequence.
ACCESSION   DAWTQS010000110 DAWTQS010000000
VERSION     DAWTQS010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.110"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(303..518)
                     /locus_tag="WNA25_002045"
     CDS             complement(303..518)
                     /locus_tag="WNA25_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824707.1"
                     /translation="MPSESPSDGICFPKRIILPQAYANPFYRIGNSKKNPFHSHAGKP
                     VFDFGYFWLFRVIYESSFPQKRESVFL"
     gene            complement(549..3317)
                     /locus_tag="WNA25_002046"
     CDS             complement(549..3317)
                     /locus_tag="WNA25_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFA9824708.1"
                     /translation="MRSSFRLKPICFYLMGVMLYHHSYAEDAGRAGSEAQIQVLEDVH
                     VKAKRVPKDKKVFTDARAVSTRQDIFKSGENLDNIVRSIPGAFTQQDKSSGIVSLNIR
                     GDSGFGRVNTMVDGITQTFYSTSTDAGRAGGSSQFGASVDSNFIAGLDVVKGSFSGSA
                     GINSLAGSANLRTLGVDDVVQGNNTYGLLLKGLTGTNSNKGNAMAAIGARKWLESGAS
                     AGVLYGHSRRGVAQNYRVGGGGQHIGNFGEEYLERRKQQYFVQEGGLKFNAGSGKWER
                     DLQRQYWKTKWYKKYEDPQELQKYIEEHDKSWRENLAPQYDITPIDPSGLKQQSAGNL
                     FKLEYDGVFNKYTAQFRDLNTKIGSRKIINRNYQFNYGLSLNPYTNLNLTAAYNSGRQ
                     KYPKGAKFTGWGLLKDFETYNNAKILDLNNTATFRLPRETELQTTLGFNYFHNEYGKN
                     RFPEELGLFFDGPDQDNGLYSYLGRFKGDKGLLPQKSTIVQPAGSQYFNTFYFDAALK
                     KDIYRLNYSTNAINYRFGGEYTGYYGSESEFKRAFGENSPAYKEHCDPSCGLYEPVLK
                     KYGKKRANNHSVSISADFGDYFMPFAGYSRTHRMPNIQEMYFSQIGDSGVHTALKPER
                     ANTWQFGFNTYKKGLLKQDDILGLKLVGYRSRIDNYIHNVYGKWWDLNGDIPSWVGST
                     GLAYTIRHRNFKDKVHKHGFELELNYDYGRFFTNLSYAYQKSTQPTNFSDASESPNNA
                     SKEDQLKQGYGLSRVSALPRDYGRLEVGTRWLGNKLTLGGAMRYFGKSIRATAEERYI
                     DGTNGGNTSNVRQLGKRSIKQTETLARQPLIFDFYAAYEPKKNLIFRAEVKNLFDRRY
                     IDPLDAGNDAATQRYYSSFDPKDKDEDVTCNADKTLCNGKYGGTSKSVLTNFARGRTF
                     LMTMSYKF"
BASE COUNT          787 a          835 c          831 g          942 t
ORIGIN      
        1 ataaaaaata acaatataaa tcaatttatt acggttttat ttttatttgg tgttgacggg
       61 caacatatca tctgcgcggt aatgatgaga ttttaggttg tgggatttat cggaaaaaca
      121 gaaacctctc cgccgccatt ctcacgaaag tgggaatccg gaaagctaaa gccacaggaa
      181 tttatcggaa atggctgaaa tttatagtgg attaacaaaa atcaggacaa ggcgacgaag
      241 ctgcagacag tacaaatagt acggcaaggc gaggcaacgc cgtacctgtt tttgttaatc
      301 cactataaaa aaactgattc ccgcttttgc gggaatgacg actcataaat tacccgaaac
      361 aaccaaaaat aaccgaaatc aaaaaccggc ttccctgcgt gggaatgaaa tggatttttc
      421 tttgagttgc cgatgcgata aaacggattg gcataggctt gcggcaggat gatgcgtttg
      481 gggaaacaaa tgccgtctga agggctttca gacggcattg cagtttttcc acaaaacgcg
      541 ggctgccttt aaaacttgta gctcatcgtc atcaagaagg tgcgtccgcg tgcgaaattg
      601 gtcaatacgc ttttgcttgt gccgccgtat ttgccgttgc acaacgtttt atcagcatta
      661 cacgttacgt cttcgtcctt gtctttcggg tcgaacgagc tgtaataacg ctgcgttgcc
      721 gcatcattgc ccgcatcgag cggatcgata taacgcctgt cgaacaggtt tttgacttcg
      781 gcgcggaaaa taaggttttt cttcggctcg taagcggcgt aaaaatcaaa aatcaaaggc
      841 tgtcgggcaa gggtttcggt ttgtttgatg gaacgcttgc ccagttgccg gacattgctg
      901 gtatttcccc cgttggtgcc gtcgatatag cgttcttcag ccgtcgcgcg gatgctcttg
      961 ccgaaatagc gcatcgcgcc gcccaaagtc agtttgttgc ccaaccagcg cgtaccgact
     1021 tccaaacgtc cgtaatctcg cggcagggcg gaaaccctgc tcagcccata accttgtttg
     1081 agttggtctt ctttggaggc attgttgggc gattcgctcg catcgctgaa attggtcggt
     1141 tgcgtgcttt tttgataggc gtaagaaagg ttggtgaaaa aacgcccata atcgtaattc
     1201 agctccagct caaaaccgtg tttgtgcact ttgtctttga aattgcggtg tcggatggtg
     1261 taggcaagcc cggtgctgcc gacccagctc ggaatatccc cgttcaaatc ccaccatttc
     1321 ccgtaaacgt tgtggatgta gttgtcaatg cggctgcggt agccgaccag tttcaatcct
     1381 aatatatcat cttgttttaa caatcctttt ttataggtat tgaagccaaa ttgccaagtg
     1441 tttgcgcgct ctggttttaa ggcggtgtga acgccggagt cgccgatttg ggaaaaatac
     1501 atttcttgga tgttgggcat acggtgtgtg cgcgaatagc cggcgaacgg catgaaataa
     1561 tcgccgaagt ccgcactaat gctgaccgaa tggttgttgg cgcgcttttt gccgtatttt
     1621 ttcaatacgg gttcataaag cccgcagctc gggtcgcaat gttccttgta tgccggcgag
     1681 ttttctccga atgcccgctt aaattcgctt tccgagccgt aatagcccgt atattcgccg
     1741 ccgaaacggt agttgattgc attggtgctg tagtttaagc ggtaaatgtc ttttttgagc
     1801 gcggcatcga agtagaacgt gttgaaatat tgactgccgg ccggttggac gatggttgat
     1861 ttttggggca gcagcccttt atcgccctta aaccgcccca aataggaata aagcccgttg
     1921 tcctgatccg gaccgtcgaa aaacagcccc aattcttcag gaaagcggtt tttgccgtat
     1981 tcgttgtgga aataattgaa gcccaaagtg gtttgcaact cggtttcgcg gggcagccgg
     2041 aaggtggcgg tgttgttgag gtcgaggatt ttcgcgttgt tgtaggtttc aaaatctttt
     2101 aaaagccccc agcctgtaaa cttcgcccct ttcggatatt tctgcctgcc cgaattgtag
     2161 gctgcggtca gattgaggtt ggtatacggg ttcaaagaca aaccgtaatt gaattgataa
     2221 ttgcggttga tgattttgcg gctgccgatt ttggtgttta aatcgcgaaa ttgcgccgtg
     2281 tatttattga atacgccgtc gtattccaat ttaaacagat tgcctgccga ctgctgcttc
     2341 aggccggacg gatcgatggg ggtgatgtcg tattgcggcg ccaggttttc ccgccagctt
     2401 ttatcatgct cttcgatgta tttttgcagt tcttgggggt cttcgtattt tttataccac
     2461 tttgttttcc agtattgcct ttgcaaatcc cgttcccatt ttccgctgcc ggcattgaat
     2521 ttcaaaccac cctcttgtac aaaatattgc tgtttgcgcc gttccagata ttcttcacca
     2581 aaatttccga tgtgctgccc gccgccgccc acgcggtaat tttgcgccac gccgcgcctg
     2641 ctgtgcccgt aaagcacacc ggcagacgct ccgctttcca gccatttgcg cgcacctatc
     2701 gccgccatcg cattgccttt gtttgaattg gtgccggtca gaccttttag cagcaggccg
     2761 taggtattat tgccctgaac gacgtcatcc acgcctaaag tccgcagatt cgccgaaccg
     2821 gcaaggctgt tgatgcctgc cgagccgctg aagctgcctt tgacgacatc cagtccggca
     2881 ataaaattgc tgtcgacaga tgcaccgaat tgagatgaac cgcctgccct gcccgcatcg
     2941 gtagaagtcg aataaaaggt ctgcgtgatg ccgtccacca tcgtattgac ccgcccgaac
     3001 ccgctgtcgc cgcgaatatt caaagacaca atgcccgagc ttttatcttg ctgtgtaaac
     3061 gcaccgggta tgctacgtac gatgttgtcg aggttttcgc cggatttgaa tatatcctga
     3121 cgggtcgata cggcacgcgc atcggtaaac acttttttgt ctttcggtac gcgcttcgcc
     3181 ttgacgtgca cgtcttccaa aacctgtatc tgcgcctcgc tgcccgcgcg ccctgcatct
     3241 tctgcataac tatgatgata tagcataaca cccataagat aaaaacaaat cggcttcaac
     3301 cggaaagaag atctcatatt ttcctcaaca ataaacaatc aatccaacat gtctaccgtt
     3361 ttcatcgaat ccgccctttc gaccacccgg cccta
//
LOCUS       DAWTQS010000111         3079 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.111, whole genome shotgun sequence.
ACCESSION   DAWTQS010000111 DAWTQS010000000
VERSION     DAWTQS010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.111"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            <1..664
                     /gene="mafB"
                     /locus_tag="WNA25_002047"
     CDS             <1..664
                     /gene="mafB"
                     /locus_tag="WNA25_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HFA9824709.1"
                     /translation="ITDSAVNPVTYAAAQQTLQGIHDLGNVSPEAQLAAASLLQDSAF
                     AVKDGINSARQWADAHPNITATAQTALAVAEAATTVWGGKKVELNPTKWDWVKNTGYK
                     KPAARHMQTVDGEMAGGNRPLESKNTVTTNNFFENTGYTEKVLRQASNGDYHGFPQSV
                     DAFSENGTVIQIVGGDNIVRHKLYIPGSYKGKDGNFEYIREADGKINHRLFVPNQQLP
                     EK"
     gene            680..982
                     /locus_tag="WNA25_002048"
     CDS             680..982
                     /locus_tag="WNA25_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824710.1"
                     /translation="MLNEIFEIYSRQGESLIGIGIREAALPVPTAIDILNLFINERIL
                     VLGGDIYIKKDNYFYQTYDNWYYEGSNLFNSINKAMHYLSQIKLENAYVSFVLKFI"
     gene            1066..1677
                     /locus_tag="WNA25_002049"
                     /pseudo
     CDS             1066..1677
                     /locus_tag="WNA25_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253822.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            1674..1991
                     /locus_tag="WNA25_002050"
     CDS             1674..1991
                     /locus_tag="WNA25_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824711.1"
                     /translation="MKKNIFHNVSLYEIIFSDNGNTLTLSFTDTIEGNYFGYIKCSNI
                     LNFKLDTNNFVDYEDKEDSLFPLFIPEIELYKYQFYSEIIIDVGIIIKISAETINFEP
                     LGK"
     gene            2061..2399
                     /locus_tag="WNA25_002051"
                     /pseudo
     CDS             2061..2399
                     /locus_tag="WNA25_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225727.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            2467..2655
                     /locus_tag="WNA25_002052"
     CDS             2467..2655
                     /locus_tag="WNA25_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mafB"
                     /protein_id="HFA9824712.1"
                     /translation="MEEVKANPQGKTPARIPPMSDTKNGWLAKDGWVKRVQNINKVEI
                     HYIENTRTGEKTDFKFKD"
     gene            2658..2981
                     /locus_tag="WNA25_002053"
     CDS             2658..2981
                     /locus_tag="WNA25_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824713.1"
                     /translation="MFLDDVNVFLDDLNTNPITDEWYMSNFADKHIKILESYEAFDIL
                     KQFVDYMIEEHDEKSEYEIMEILRQLKYQADTNEKFYTNTQKQKIVELYKQEISQDIL
                     NEIFR"
BASE COUNT         1140 a          517 c          624 g          798 t
ORIGIN      
        1 gattacagac agcgcggtaa acccggtaac ctatgcggca gcacagcaaa ctttacaggg
       61 tattcacgat ttaggaaatg taagtccgga agcacaactt gctgccgcga gcctattaca
      121 ggacagtgct tttgcggtaa aagacggcat caattccgcc agacaatggg ctgatgccca
      181 tccgaatata acagcaacag cccaaactgc ccttgccgta gcagaggccg caactacggt
      241 ttggggaggt aaaaaagtag aacttaaccc gaccaaatgg gattgggtta aaaataccgg
      301 ctataaaaaa cctgctgccc gccatatgca gactgtagat ggggagatgg caggggggaa
      361 tagaccgctg gaatcaaaaa atactgtaac aacgaataat ttttttgaaa ataccggtta
      421 tacagagaaa gtattaaggc aagcatcgaa tggtgattat catggttttc cgcaatcagt
      481 agatgctttt tctgaaaatg gaacagtaat acaaatagtt ggcggagata atattgtaag
      541 gcataaactt tatatccctg gatcttataa aggaaaagat ggaaattttg aatatatcag
      601 agaagcagat ggaaaaatta atcatagatt atttgtccct aaccaacaac ttcctgaaaa
      661 atagtagata gagattaata tgttaaatga aatttttgaa atttattcga gacaagggga
      721 atctttgata ggaattggaa ttagagaagc cgcattaccc gtccccactg caatagatat
      781 attaaattta tttataaatg aaagaatact tgtattgggg ggagatattt atatcaagaa
      841 agataattat ttttatcaaa catatgataa ttggtattac gagggaagta atttatttaa
      901 cagtatcaac aaagcaatgc attatttatc tcaaataaaa ttagagaatg catacgtgtc
      961 ttttgttttg aaatttatct aacaaaggaa gcacaagaat aggtttatag taaaacatca
     1021 agatgttgaa aatgctgggt tttaacctaa cctacgctga ccggatcaga tacagccgct
     1081 cagcagactc tacaaggtat taatgattta ggaaatttaa gtccggaagc acaacttgcc
     1141 gccgcgagcc tattacagga cagtgccttt gcggtaaaag acggcatcaa ttccgccaga
     1201 caatgggctg atgcccatcc gaatataaca gcaacagccc aaactgccct tgccgtagca
     1261 gaggccgcag gtacggtttg gagaggtaaa aaagtagaac ttaacccgac taaatgggat
     1321 tgggttaaaa ataccggcta taaaaaacct gctgcccgcc atatgcagac tttagatggg
     1381 gagatggcag gaggaaacaa gccagttgtt aaatctatta gaccaactac gcgagatgaa
     1441 ttacgtcaag cattgcaaga acaaggtttt agacgtactg gttcagatgc ggctcaatat
     1501 gaaacatgga aaggtcctga tggcgtgaaa atagatattc gtccaaatgg agaggttata
     1561 agaacccaaa gagtgccgcg aaccgatggt gtacagggaa aatatccgca acgacaagat
     1621 tatgaaggca atccattgcc aaataatcat catcattctg gatattttgt caaatgaaaa
     1681 aaaatatttt tcacaatgta agcctttatg aaataatctt ttccgataat ggaaataccc
     1741 ttacattatc ttttacagat acaattgaag gtaattattt cggatatatc aaatgcagta
     1801 atattttgaa ttttaaatta gatacaaata atttcgtaga ttatgaggat aaggaagata
     1861 gcttgtttcc cttgtttata cccgaaatag agctatataa ataccaattt tatagtgaaa
     1921 ttattattga tgtagggatt attataaaaa tatctgctga aacaattaat tttgagccac
     1981 tgggaaaata gtaactgctt tcccagcagc cgtagcaact gtatttttac ccgacggggt
     2041 aaaaatacag ttgctacatc tctgcgcagc agactctaca aggtattaat aattcaggaa
     2101 aattaagtcc ggaagcacaa cttgccgccg tgagcctatt acaggacagt gcttttgcgg
     2161 taaaagacgg tatcaactct gccagacaat gggctgatgc ccatccgaac attacagcta
     2221 ctgcccaaac tgcccttgcc gtagcagagg ccgcaggtac ggtttggaga ggtaaaaaag
     2281 tagaacttaa cccgactaaa tgggattgag ttaaagatac cggctatgaa aaacctactg
     2341 cccgccatat gcagactgta gacagggaaa tggctggggg gaaacaaatc attaaaaata
     2401 gggacacaat ctgttgaaaa atcaaccggt cgtacaatac ctaataattt aaaggaacaa
     2461 ttagcaatgg aagaagttaa ggcaaaccca cagggcaaaa ctcctgcgag aatacctcct
     2521 atgtccgata ctaaaaatgg ttggttggca aaagacggct gggttaagcg cgttcaaaat
     2581 ataaacaaag ttgagataca ttacattgaa aatacaagaa ccggtgagaa aacagatttc
     2641 aagtttaagg attagccatg tttttagatg atgtaaatgt ttttttagat gatttaaata
     2701 ccaatccaat cactgacgaa tggtatatgt ccaattttgc cgataaacat attaaaattt
     2761 tggaaagtta cgaagccttt gatattctaa aacaatttgt tgattacatg attgaagaac
     2821 atgatgaaaa atcagaatat gaaatcatgg aaatattgag acaattaaaa tatcaagcag
     2881 ataccaacga aaaattttat acaaatacac agaaacagaa aattgtagaa ttatataaac
     2941 aagaaattag tcaggatatt ttaaatgaaa tctttagata aactatcaat atagaagaaa
     3001 atccttggaa aaaataaaat gataatcaaa cataatggaa atatacataa aatagccaga
     3061 atgactggaa ataaaaata
//
LOCUS       DAWTQS010000112         2924 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.112, whole genome shotgun sequence.
ACCESSION   DAWTQS010000112 DAWTQS010000000
VERSION     DAWTQS010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.112"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            141..368
                     /locus_tag="WNA25_002054"
     CDS             141..368
                     /locus_tag="WNA25_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFA9824714.1"
                     /translation="MNQKQTQCKQIVDYIRNKGCITSLEAYQNLKVTQLAARITDLEG
                     RGFVFAKPKYKVGNCKNPVAHYSIAKSGIEP"
     gene            365..502
                     /locus_tag="WNA25_002055"
     CDS             365..502
                     /locus_tag="WNA25_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824715.1"
                     /translation="MSREQRIRGARLPVSAYMQAEGAGKAREALDGRVEKVKGVDDEA
                     V"
     gene            486..1550
                     /locus_tag="WNA25_002056"
     CDS             486..1550
                     /locus_tag="WNA25_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824716.1"
                     /translation="MMKPSESLRAAGRPIAYYPKLAKPLGGVNAAILFGHFFYWNDKT
                     QYESGIYRTAEEIEIETGLSVQEQRTARAKLRERGVLIETEKRIEHRIYYKLNLDAFD
                     DLMLQHSGGGESTAPKCNINSPELQNQHSGGGESTAPKCNINSPELQNQHSGSEESTA
                     VIRTEDLTEDLVVYTPLPPNAGNGKGGLNADAFVSADAETCGRETGEPTSPKAESDSN
                     GNGGLSGKPKNANVPRRRKTHGVPLQEIADLYNEVLGGRLPSVQVLNDTRKRAIANRW
                     CEMLGTAAPNGKVRFGDKETGLAWFAGFFRKVAMNPFWMGENQTGFAVGFDWIFKAGN
                     FVKILEWHPPKTNQAARGRA"
     gene            1547..2908
                     /locus_tag="WNA25_002057"
     CDS             1547..2908
                     /locus_tag="WNA25_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
                     /protein_id="HFA9824717.1"
                     /translation="MNRIEETEAVQSLASVGAEQNILGGILIEPTAIARCAILTPEKF
                     YQAQHRIIFRALLDMAAANEPIDIITLNDKLEARGEAENAGGLAYLIDLNQNTPSAKN
                     ISRYVGIVNDRFVERGLLKASAAIEKIAVSKDGGTVAEKLSKAADELAAAGKDAVKRE
                     TKTFGQTVEDLIGGLDKRLDGVRFGLPTGLMKLDGMTGGLPDGNLIVIAARPSMGKTV
                     LAENIARFALKQGKAVHFQSYEMSAVELARRGMAAECNIPMQNLKTGNLTQSDYANMP
                     IYVSQAKEWKFDVNCDLLNVDELCFLAKEKKLTTGLDLLVVDHLHIMPRAGRDEVAEL
                     GNISRRLKNLAAELNIPVVLVAQLNRGNTKQADKRPNMADIRGSGAIEQDANIIIMPH
                     RESYYDGNENPSIAELIIAKNRDGETGTVVCGWKGQFMKFEEEPDLAWQAPKHDEYDP
                     YSV"
BASE COUNT          810 a          683 c          889 g          542 t
ORIGIN      
        1 cggttgtaac ggggtgacgg caaatcggaa agacggctga ccgcccggac agacgggcgg
       61 ccgataaaga aaaacccgca cggggcgggt aatccccctg aattgcaggg aagcggttca
      121 ggtaacggcg aaaggcgatt atgaatcaaa aacaaacgca atgcaaacaa attgtcgatt
      181 acatccgtaa caagggatgc atcacatccc ttgaggctta tcagaacctg aaggtgacgc
      241 agcttgcggc acggataacc gacttggaag gcaggggctt cgtgtttgcc aagccgaaat
      301 acaaggtcgg taactgtaaa aatcccgttg cccattactc aatcgcaaag tcaggaattg
      361 aaccatgagc cgggaacaaa gaatacgcgg agcccgcttg ccggtgtcgg cttatatgca
      421 ggcggaaggc gcgggcaagg cacgggaagc gttggacggg cgggtcgaaa aagtgaaagg
      481 ggttgatgat gaagccgtct gaaagtttga gagcggcagg caggccaatc gcgtattacc
      541 cgaaattggc aaagcctttg ggcggtgtaa atgcggcaat attattcggg catttcttct
      601 actggaacga taagacgcag tatgaatcag gcatttaccg aacagcggaa gaaattgaaa
      661 ttgaaaccgg gctgtccgtt caggaacaaa gaacggcacg ggcaaagctg agggaacgcg
      721 gcgtattgat tgagactgaa aaacgaattg aacaccgcat ttactacaaa ctgaatttag
      781 acgcttttga tgatttgatg ttgcaacatt cggggggtgg ggaatcaaca gccccgaaat
      841 gcaatatcaa cagccccgaa cttcaaaatc aacattcggg gggtggggaa tcaacagccc
      901 cgaaatgcaa tatcaacagc cccgaacttc aaaatcaaca ttcggggagt gaggaatcaa
      961 cagccgttat aagaacagaa gatttaacag aagatttagt agtatatacc cccttacccc
     1021 caaacgccgg aaacggcaaa ggcggtttga acgctgacgc gtttgtttcc gctgacgcgg
     1081 aaacgtgcgg gcgggaaacc ggcgaaccga cttcgccgaa ggccgaaagc gacagtaacg
     1141 gcaacggcgg cctttcggga aaaccgaaaa atgcgaatgt tccgcgccgc cgcaaaaccc
     1201 acggcgtacc gcttcaggaa atcgccgatt tgtacaacga agttttgggc ggccggttgc
     1261 caagcgtcca agtgctgaac gacacgcgca aacgggcgat tgcaaaccgc tggtgcgaga
     1321 tgctgggaac ggcggcgcca aacggcaagg tgaggttcgg ggacaaggaa accggtttgg
     1381 cctggtttgc cggtttcttc cggaaagtgg cgatgaaccc gttttggatg ggcgaaaacc
     1441 aaacagggtt tgcggtcggc ttcgattgga ttttcaaggc gggcaatttc gtcaaaatcc
     1501 ttgaatggca tccgcctaaa acgaaccagg cggcaagggg aagggcatga accgaatcga
     1561 ggaaacggaa gcggtccaat cactggccag cgtaggggcg gaacagaaca ttttgggcgg
     1621 catcttgatt gaaccgacgg cgattgcgcg gtgcgcaatc ctgacccctg aaaagtttta
     1681 ccaggcgcaa cacaggatta ttttccgcgc tctgctggat atggcggcgg caaacgagcc
     1741 tatcgacatc atcacgctga acgacaagct ggaagcgcgc ggcgaggcgg aaaacgcggg
     1801 tggcctggct tacttgatag acctgaacca aaacacccca agcgcgaaga atatcagccg
     1861 gtacgttggg attgtgaacg acaggtttgt cgagcgcggc ttgctgaagg cttcggcggc
     1921 gattgaaaaa atcgcggttt ccaaagacgg cgggacggtc gcagaaaagc tgtctaaggc
     1981 ggccgacgaa ttggcggcag ccggcaaaga cgcggtaaag cgtgaaacca agacattcgg
     2041 ccagaccgtt gaggatttga ttggcggttt ggacaaaagg cttgacggcg tgcgtttcgg
     2101 attgcctacc ggcttgatga agcttgacgg gatgaccggc ggtttgccgg atggaaacct
     2161 gattgtgatt gcggcgcgtc cgtctatggg taaaacggtt ttggcggaaa acattgcgcg
     2221 attcgcgctg aagcagggca aggcagttca tttccaaagc tacgaaatga gcgcggtaga
     2281 gctggcaagg cgcggcatgg cggctgagtg caatatcccc atgcagaacc tgaaaaccgg
     2341 caatctgacg caaagcgatt acgccaatat gccgatttac gtcagccaag cgaaagagtg
     2401 gaagtttgac gtgaactgcg acctgctcaa cgttgacgag ctttgctttt tggctaagga
     2461 gaaaaaactc actaccggct tggatttgtt ggttgtcgat caccttcaca tcatgccaag
     2521 ggcagggagg gacgaggtgg cggagttggg gaatatatcg cgccgtttga aaaacttggc
     2581 ggcagagctg aatatccccg tcgtcttggt tgcccagttg aacaggggaa acacaaagca
     2641 ggcagacaaa cgcccgaaca tggcagacat tcgcggcagc ggcgcgattg agcaagacgc
     2701 aaacatcatc atcatgccgc accgcgaaag ctactacgac ggaaacgaga atccgagcat
     2761 tgccgagctg attatcgcca agaaccggga cggcgaaacg ggaacggtgg tttgcggctg
     2821 gaaagggcaa tttatgaagt tcgaggaaga gcctgatttg gcatggcaag cccccaaaca
     2881 tgatgaatat gacccttaca gtgtctagtg cgggaggccg gtaa
//
LOCUS       DAWTQS010000113         2891 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.113, whole genome shotgun sequence.
ACCESSION   DAWTQS010000113 DAWTQS010000000
VERSION     DAWTQS010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.113"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            444..662
                     /locus_tag="WNA25_002058"
     CDS             444..662
                     /locus_tag="WNA25_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824718.1"
                     /translation="MANSNSGHSKKLRAATAAAATKAKLASGEYRQFSVQGRAEDVEL
                     ILAAVEKAGGSRVQALAKICRRYLEGLS"
     gene            complement(679..1038)
                     /locus_tag="WNA25_002059"
     CDS             complement(679..1038)
                     /locus_tag="WNA25_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824719.1"
                     /translation="MQVLLGEDFKRALKNYPKEDRRKIAEFIAHVQQNGLSGLPGRNK
                     SSDNVPADDPQWLEKVRFAQRHNLWHYHIGIPKYNGGRYGDLTSAYILHYTLCDGFIK
                     IIGFDRHPPFILPDIPK"
     gene            complement(1039..1578)
                     /locus_tag="WNA25_002060"
     CDS             complement(1039..1578)
                     /locus_tag="WNA25_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SocA family protein"
                     /protein_id="HFA9824720.1"
                     /translation="MLNAYDVADFFLSPFEEEDGEQISNLKLQKLLYYAQGYALAILN
                     RPLFAENIEHWQHGPVVPCIYRTYKKYGGSPLPAAHIEPDKYADEELVVLNRVRKEQG
                     CYTAWALRNKTHQEAPWIQTRQGEVIGIALMGEYFRHALPQTDYNFNLEKLKTAVEDS
                     FVSVPHFNGADDLEKWLEQ"
     gene            complement(1738..2442)
                     /locus_tag="WNA25_002061"
     CDS             complement(1738..2442)
                     /locus_tag="WNA25_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFA9824721.1"
                     /translation="MTNRDLKAIRIDNLKRFFENKKLPVKDKSLLSQLMSGKASFGEK
                     VSRRLEEEYGMGSLYLDSVPSEPTPVLINPDLPHEVKDIHRPMMWSSNDPLPDDDYVF
                     VPYLKESCFKGGAGAYEIPDYNGYRLPFGKSTLRRKGINPDNVFCCTLTGDSMEEKIA
                     EDAAIAVDTGETAIRDGKIYAFAQDGMFRVKYLIRQPGNSVLIRSHNSGFYPDETAPL
                     DSLSVIGRVFWWSVLD"
     gene            2597..2785
                     /locus_tag="WNA25_002062"
     CDS             2597..2785
                     /locus_tag="WNA25_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFA9824722.1"
                     /translation="MSEQRGAKTEYAKKLGLSLSFLWQIENGRAVTPKRLYKDVMLLT
                     KNKVSISELISEFRVCPK"
BASE COUNT          720 a          626 c          739 g          806 t
ORIGIN      
        1 tttagtaaac acttaacatc tagtcaagta aaatgtttag tgattattta ataaatactg
       61 aacattttga ttattaaaag aatttatttt tgagatttcg caggcgcaaa aaaaaccgcc
      121 tattaaggcg gttgtttagg aaagaatgcc ctctgcgcgt gtagggtatt tcgatacttg
      181 cgtttattat atcatatgat ataataaact catagattag gaaatgaaga aaccccgtat
      241 cagccgatac ggggcaacct gaaagagaaa ggaggcaatg tgattaagaa aatcctaatc
      301 tgtgtgattt tgtatttcct tgcaagctcc ccagcgtggt agggaaatac aaaacaagtg
      361 attaacgatt aaccatttgc agggcagtta aggaggctgc ccttcctctt atctttccca
      421 tactctacta taaggattta aaaatggcaa attccaacag cgggcattct aaaaaattgc
      481 gcgcggcaac ggcggcggcg gcgacaaagg caaaactggc aagcggcgaa taccggcagt
      541 tttcagtgca ggggcgtgcc gaagacgtgg agcttatcct tgccgccgtg gaaaaagccg
      601 ggggcagccg cgtacaggct ttggcaaaga tttgcaggcg gtatctcgag gggctgtctt
      661 aaggggggta agacaaggtt atttgggaat atcgggcagg ataaaaggcg ggtgtctgtc
      721 aaaaccgatg attttgataa agccgtcgca aagggtatag tgaaggatgt aggcggatgt
      781 caaatcgcca tacctgccgc cgttgtattt gggaatgccg atgtgatagt gccaaaggtt
      841 gtgccgttgg gcaaatcgga ctttttccag ccattgagga tcgtctgccg gtacgttgtc
      901 gctggatttg ttcctgccgg gtaaccctga taagccgttc tgttggacgt gtgcgataaa
      961 ttccgcaatt tttctgcggt cttctttggg gtagtttttc aacgctcttt tgaagtcttc
     1021 gccaagcagc acctgcattt actgctccaa ccatttttcc aagtcgtcgg cgccgttgaa
     1081 atgcgggacg ctgacaaagc tgtcttcaac ggctgttttg agtttttcaa gattgaaatt
     1141 gtaatccgtc tgcggcagcg catggcggaa atattccccc atcagcgcaa tccctatgac
     1201 ttcgccctgc cgggtctgta tccacggcgc ttcctgatgg gttttattgc gtaatgccca
     1261 agcggtgtag cagccctgct ctttacggac acggttgagg acaaccaact cttcgtccgc
     1321 atatttgtcc ggttcgatat gggccgcagg caatgggctg ccgccgtatt ttttgtaggt
     1381 gcggtaaatg caggggacta ccggaccgtg ctgccagtgt tcgatatttt cggcaaacag
     1441 ggggcggtta agtatggcaa gggcgtagcc ttgtgcgtaa tacaggagtt tttgaagttt
     1501 gagattggag atttgctccc cgtcctcttc ttcaaaaggg gaaaggaaga aatctgccac
     1561 gtcgtacgcg ttcagcattt tctgtttccg ttgttatttt tctgtatatt aaggccgccg
     1621 gcccattatt tcaagaaata atttgtgcaa attatccggt atcggcaggc tgtccgcgcc
     1681 gtcggcatat cgggcggata tatttgaaaa atacttgccc tgccgtttca gacggcatca
     1741 atccaacacg ctccaccaaa agaccctgcc gataacggac aggctgtcca aaggggcggt
     1801 ttcgtcggga tagaagccgc tgttgtggct gcgtatcagc acgctgttgc cgggctgccg
     1861 tatcaggtat ttgacgcgga acatgccgtc ttgggcgaag gcgtatattt tgccgtcccg
     1921 tatggcggtt tcgcccgtat ctacggcgat tgccgcgtct tctgcgattt tttcctccat
     1981 gctgtcgccg gtcagggtgc agcagaagac gttgtcgggg ttgatgcctt tgcgccttaa
     2041 cgttgatttg ccgaacggca ggcggtagcc gttgtagtcg gggatttcat acgcgcccgc
     2101 cccgcctttg aagcagctct ctttgaggta ggggacgaaa acataatcat cgtcgggcag
     2161 cgggtcgttg ctgctccaca tcatcgggcg gtggatgtct ttgacttcgt ggggcaggtc
     2221 ggggttgatg aggacgggcg ttggttcgct cggaacggaa tccaagtaca gactgcccat
     2281 cccatattct tcttcaagcc gtctgcttac cttttcgcca aaagatgctt taccgctcat
     2341 caattgagat aaaaggcttt tgtctttaac cggcaacttt ttattttcaa agaatctttt
     2401 taaattatcg atacggatgg ccttcagatc tctattagtc attttcgcgc tccattcaga
     2461 ttgggattca attttattta gtttctgcta aacaaacaaa tattaaattt tcttgcctat
     2521 gcgtttaata tttgctaaac tcattaaata tttacggtga aactacagaa tgactcattc
     2581 agaatttatt aaaacgctgt cagaacagcg tggtgcaaaa acggaatacg ctaaaaaact
     2641 tggcttatcc ctgtcttttc tatggcagat agaaaacgga cgggcagtaa cgccaaagcg
     2701 tctttataaa gacgttatgt tgttgacaaa aaataaggtt tctatttctg aattaatttc
     2761 ggagttcagg gtttgtccca aataaaaagc ccgtcgggtc agatgttaat tgatataacc
     2821 gaattgaagc ggaagtcatc cgcaatttac cggaaaggaa aaaaatgaag aagcaggaca
     2881 gaaaccgcct g
//
LOCUS       DAWTQS010000114         2747 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.114, whole genome shotgun sequence.
ACCESSION   DAWTQS010000114 DAWTQS010000000
VERSION     DAWTQS010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.114"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(46..1083)
                     /locus_tag="WNA25_002063"
     CDS             complement(46..1083)
                     /locus_tag="WNA25_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFA9824723.1"
                     /translation="MKKSLIALTLAALPVAATADVTLYGTIKAGVQTYRSVEHTKGKV
                     SKVETGSEIADFGSKIGFKGQEDLGNGLKAVWQLEQGASVAGTNTGWGNKQSFVGLKG
                     GFGTIRAGSLNSPLKNTNNNVNAWESGKFTGNVLEISGMAQREHRYLSVRYDSPEFAG
                     FSGSVQYAPKDNSGSNGESYHVGLNYRNNGFFAQYAGLFQRYGEGTKMEGYAYNIPSL
                     FVEKLQVHRLVGGYDNNALYASVAAQQQDAKLYGAMSGNSHNSQTEVAATVAYRFGNV
                     TPRVSYAHGFKGTVDDANHDNTYDQVVVGAEYDFSKRTSALVSAGWLQEGKGADKIVS
                     TASAVVLRHKF"
     gene            complement(1600..2397)
                     /gene="truA"
                     /locus_tag="WNA25_002064"
     CDS             complement(1600..2397)
                     /gene="truA"
                     /locus_tag="WNA25_002064"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFA9824724.1"
                     /translation="MDTAQKQRWAITLSYDGSRFYGWQKQAGGVPTVQAALETALARI
                     AGESVATTVAGRTDTGVHAAAQVVHFDITAARPQQAWVRGVNAHLPEGIAVLHARQVA
                     PGFHARFDAYGRHYRYLLESAPVRSPLLKNRAGWTHLKLDIEPMRRAAALLIGEQDFS
                     SFRAAGCQAKSPVKTIYRTDLTQSAGLIRLDLHGNAFLHHMVRNIMGALVYVGSGRLS
                     VEGFAALIQERSRLKAPPTFMPDGLYLTGVDYPGAYGIVRPQIPEWL"
BASE COUNT          553 a          652 c          833 g          709 t
ORIGIN      
        1 agcctgtctg gcgacaggct ttttgttgat accaatcttt gcagattaga atttgtggcg
       61 cagaacgacg gcgctggcag tcgatacgat tttgtctgcg cctttgcctt cttgcaacca
      121 gccggcagaa accaaggcag aagtgcgttt ggagaagtcg tattccgcac cgacaaccac
      181 ttggtcgtaa gtattgtcgt ggtttgcatc atcaacagtg cctttgaagc cgtgggcgta
      241 agaaacacgg ggcgttacgt tgccgaaacg gtatgctacg gtagcggcaa cttcggtttg
      301 agagttgtgc gaattaccgc tcattgctcc atacaatttg gcatcttgtt gttgtgcggc
      361 tacggaggcg tacagggcat tattgtcgta accgccgacc aaacggtgaa cttgcagttt
      421 ttcaacaaac aaactgggga tattatatgc atatccttcc attttagtgc cttcgccgta
      481 tctttggaac aagccggcgt attgtgcgaa gaagccgttg tttcggtagt tcaagccaac
      541 gtggtaagat tcgccgtttg accctgaatt gtctttaggt gcgtattgta cgctgccgct
      601 gaagccggca aattcgggag aatcgtagcg tacggacagg tagcggtgtt cccgttgggc
      661 cattccgctg atttccagca cattgccggt aaatttgccg gattcccaag cattgacgtt
      721 gttgttggtg tttttcaggg ggctgttcag gctaccggcg cggatggtgc cgaagccgcc
      781 cttcaagccg acgaaggatt gtttgttgcc ccagccggtg ttagtgccgg cgacggaggc
      841 gccttgttcc aactgccaaa cggccttcag gccgttgccg aggtcttctt ggcctttgaa
      901 gccgattttt gaaccgaagt cggcgatttc gctgccggtt tccactttac ttaccttgcc
      961 ttttgtatgt tctacagaac ggtaagtttg tacgccggct ttgatggtgc cgtacagggt
     1021 gacatcggcc gttgccgcaa caggaagggc tgccaaagtc agggcaatca gggatttttt
     1081 cattgctgta ttcctttttt ggttaagaaa tttaagcagg ccgggctttc caagccgctt
     1141 agctttgcat ttaccgccga ggggctcggc ggttacatgt ctgaaactat agatgttcct
     1201 gtctgaaaaa acaaacttct gcctgttttt gtgtggaatc cgatgtgcat tttgaaggac
     1261 ggatagagat attacgggta ttttttgttt tgtaacatat ggttatttaa aatttttaag
     1321 atttgcattt ttacaacact tactcgggag ggtattggag ggcattgcaa accgggggtt
     1381 atagcggatt aacaaaaacc ggtacggcgt tgccccgccc cggcccaaag ggaacggttc
     1441 cctaaggcgc ccaagcaccg ggcgaaccgg ttccgtacca tttgtactgt ctgcggcccg
     1501 ccgccttgtc ctgatttttg ttaatccact atagtgtcaa cattgaaaaa gattgcctga
     1561 cagtttgtcc gatttcaaaa tctccgcgac aagaatgttt taaagccatt cggggatttg
     1621 ggggcggacg atgccgtatg ccccgggata gtcgacgccg gtcaggtaaa gcccgtcggg
     1681 catgaaggtc ggtggggctt tgaggcggct gcgttcttga atcagtgcgg cgaagccttc
     1741 gacgctgagc ctgccgctgc cgacataaac gagcgcgccc atgatgttgc gtaccatatg
     1801 gtgcaaaaag gcgttgccgt gcaaatcgag gcggatcagt cctgcgcttt gggtaaggtc
     1861 ggtgcggtag atggttttga cgggggattt tgcctggcac ccggcggcgc ggaagctgga
     1921 gaagtcttgt tcgccgatca ataaggcggc agcccggcgc atcggttcga tgtcgagttt
     1981 gaggtgtgtc cagcctgccc tgtttttgag caggggggaa cggacggggg cggattcgag
     2041 caggtagcgg tagtgccgtc cgtatgcgtc gaagcgggcg tgaaatccgg gtgcgacctg
     2101 tcgggcgtgc aaaacggcaa tgccttcggg caggtgggca tttacgccgc gcacccatgc
     2161 ctgttgggga cgggcggcag ttatgtcaaa gtggacgact tgggcggcag cgtgcacgcc
     2221 ggtgtcggtc ctgccggcaa ctgtggtggc gacagattct cctgcgattc gggcgagtgc
     2281 ggtttccaat gccgcctgaa cggtcggtac gccgcccgcc tgtttctgcc agccgtaaaa
     2341 gcggctgccg tcgtaggata gggttattgc ccagcgttgt ttttgtgcgg tatccatcgg
     2401 gtttgggatt cggataaatg ttcagactgc attgtatcgc agattttgca gggaaacggc
     2461 aaacgcccgg ggcgcgcagc gttgtttggg gagttgttcg ggggttggat gcagttgcta
     2521 cgaatcgcta tcctgtgaat tcaccctgtc aggagtgccc gaaccgtcat tcccgcgcag
     2581 gcgggaatct aggacgtaaa aatctaaaga aaccatttta tccgataagt ttccgcaccg
     2641 acaaaaccag attcccgcct gcgcgggaat gacggcggcg ggaatcggtg gattcggcgg
     2701 agttggtgga tttggcgggc tgaagcccgc ccttgtatat cggaact
//
LOCUS       DAWTQS010000115         2735 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.115, whole genome shotgun sequence.
ACCESSION   DAWTQS010000115 DAWTQS010000000
VERSION     DAWTQS010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.115"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            <1..689
                     /locus_tag="WNA25_002065"
     CDS             <1..689
                     /locus_tag="WNA25_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFA9824725.1"
                     /translation="IAMGAWLEVLSEEKDGNRLARHHKHGEIWKKPTRHEDIAAVFPF
                     GNPIHNNTMIMRRSVIDGGLRYDTGRDWAEDYQFWYDVSKLGRLAYYPEALVKYRLHA
                     NQVSSKHSVRQHEIAQGIQKTARNDFLQSMGFKTRFDSLEYRQTKAAAYELPEKDLPE
                     EDFERARRFLYRCFKRTDTPPSGAWLDFAADGRMRRLFTLRQYFGILYRLIKNRRQAR
                     SDSAGKEQEI"
     gene            689..1528
                     /locus_tag="WNA25_002066"
     CDS             689..1528
                     /locus_tag="WNA25_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HFA9824726.1"
                     /translation="MQNHVISLASAAERRAHIADTFGSRGIPFQFFDALMPSERLEQA
                     MAELVPGLSAHPYLSGVEKACFMSHAVLWEQALDEGLPYIAVFEDDVLLGEGAEQFLA
                     EDTWLQERFDPDSAFVVRLETMFMHVLTSPSGVADYGGRAFPLLESEHCGTAGYIISR
                     KAMRFFLDRFAVLPPERLHPVDLMMFGNPDDREGMPVCQLNPALCAQELHYAKFHDQN
                     SALGSLIEHDRRLNRKQQWRDSPANTFKHRLIRALTKIGREREKRRKRREQTIGKIIV
                     PFQ"
     gene            1540..2460
                     /locus_tag="WNA25_002067"
     CDS             1540..2460
                     /locus_tag="WNA25_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFA9824727.1"
                     /translation="MDIVFAADDNYAAYLCVAAKSVEAAHPDTEIRFHVLDAGISEEN
                     RAAVAANLRGGGNIRFIDVSLEDFAGFPLNIRHISITTYARLKLGEYIADCDKVLYLD
                     TDVLVRDGLKPLWDTDLGGNWVGACIDLFVERQEGYKQKIGMADGEYYFNAGVLLINL
                     KKWRRHDIFKMSCEWVEQYKDVMQYQDQDILNGLFKGGVCYANSRFNFMPTNYAFMAN
                     GFASRHTDPLYLDRTNTAMPVAVSHYCGSAKPWHRDCTVWGAERFTELAGSLTTVPEE
                     WRGKLAVPPTKRMLQRWRKKLSARFLRKIY"
     gene            2520..>2735
                     /locus_tag="WNA25_002068"
     CDS             2520..>2735
                     /locus_tag="WNA25_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFA9824728.1"
                     /translation="MQPLVSVLICAYNVEKYFAQSLSAVVGQTWRNLDILIVDDGSTD
                     GTPAIARRFQEQDGRIRIISNPRNLGFI"
BASE COUNT          659 a          721 c          768 g          587 t
ORIGIN      
        1 tcattgcgat gggcgcgtgg ctggaagttt tgtcggaaga aaaggacggc aaccggctgg
       61 cgcggcacca caaacacggc gaaatttgga aaaagccgac ccggcacgaa gacatcgccg
      121 ccgttttccc tttcggcaac cccatacaca acaacacgat gattatgcgg cgcagcgtca
      181 ttgacggcgg tttgcgttac gacaccgggc gggattgggc ggaagattac caattttggt
      241 acgatgtcag caaattgggc aggctggctt attatcccga agccttggtc aaataccgcc
      301 ttcacgccaa tcaggtttca tccaaacaca gcgtccgcca acacgaaatc gcgcaaggca
      361 tccaaaaaac cgccagaaac gattttttgc agtctatggg ttttaaaacc cggttcgaca
      421 gcctagaata ccgccaaaca aaagcagcgg cgtatgaact gccggagaag gatttgccgg
      481 aagaagattt tgaacgcgcc cgccggtttt tgtaccgatg cttcaaacgg acggacacgc
      541 cgccctccgg cgcgtggctg gatttcgcgg cagacggcag gatgaggcgg ctgtttacct
      601 tgaggcaata cttcggcatt ttgtaccggc tgattaaaaa ccgccggcag gcgcggtcgg
      661 attcggcagg gaaagaacag gagatttaat gcaaaaccac gttatcagct tagcttccgc
      721 cgcagagcgc agggcgcaca ttgccgatac cttcggcagt cgcggcatcc cgttccagtt
      781 tttcgacgca ctgatgccgt ctgaaaggct ggaacaggcg atggcggaac tcgtccccgg
      841 cttgtcggcg cacccttatt tgagcggagt ggaaaaagcc tgctttatga gccacgccgt
      901 attgtgggaa caggcgttgg acgaaggctt accgtatatc gccgtatttg aagatgatgt
      961 cttactcggc gaaggcgcgg agcagttcct tgccgaagat acttggctgc aagaacgctt
     1021 tgaccccgat tccgcctttg tcgtccgctt ggaaacgatg tttatgcacg tcctgacctc
     1081 gccctccggc gtggcggact acggcgggcg cgcctttccg cttttggaaa gcgaacactg
     1141 cgggacggcg ggctatatta tttcccgaaa ggcgatgcgt tttttcttgg acaggtttgc
     1201 cgttttgccg cccgaacgcc tgcaccctgt cgatttgatg atgttcggca accctgacga
     1261 cagggaagga atgccggttt gccagctcaa tcccgccttg tgcgcccaag agctgcatta
     1321 tgccaagttt cacgaccaaa acagcgcatt gggcagcctg atcgaacatg accgccgcct
     1381 gaaccgcaaa cagcaatggc gcgattcccc cgccaacaca ttcaaacacc gcctgatccg
     1441 cgccttgacc aaaatcggca gggaaaggga aaaacgccgg aaaaggcgcg aacagacaat
     1501 cggcaaaatt attgtgcctt tccaataaaa ggagaagaga tggacatcgt atttgcggca
     1561 gacgacaact atgccgccta tctttgcgtt gcggcaaaaa gcgtggaagc ggcccatccc
     1621 gatacggaaa tcaggttcca cgtcctcgat gccggcatca gtgaggaaaa ccgggcggcg
     1681 gttgccgcca atttgcgggg ggggggtaat atccgcttta tagacgtaag cctcgaagat
     1741 ttcgccggct tccccttaaa catcaggcac atttccatta cgacttatgc ccgcctgaaa
     1801 ttgggcgaat acattgccga ttgcgacaaa gtcctgtatc tggatacgga cgtattggtc
     1861 agggacggcc tgaagccctt atgggatacc gatttgggcg gtaactgggt cggcgcgtgc
     1921 atcgatttgt ttgtcgaaag gcaggaagga tacaaacaaa aaatcggtat ggcggacgga
     1981 gaatattatt tcaatgccgg cgtattgctg atcaacctga aaaagtggcg gcggcacgat
     2041 attttcaaaa tgtcctgcga atgggtggaa caatacaagg acgtgatgca atatcaggat
     2101 caggacattt tgaacgggct gtttaaaggc ggggtgtgtt atgcgaacag ccgtttcaac
     2161 tttatgccga ccaattatgc ctttatggcg aacgggtttg cgtcccgcca taccgacccg
     2221 ctttacctcg accgtaccaa tacggcgatg cccgtcgccg tcagccatta ttgcggctcg
     2281 gcaaagccgt ggcacaggga ctgcaccgtt tggggtgcgg aacgtttcac agagttggcc
     2341 ggcagcctga cgaccgttcc cgaagaatgg cgcggcaaac ttgccgtccc gccgacaaag
     2401 cgtatgcttc aaagatggcg caaaaagctg tctgccagat tcttacgcaa gatttattga
     2461 cggggcaggc cgtctgaagc cttcagacgg catcggacgt atcggaaagg agaaacggat
     2521 tgcagccttt agtcagcgta ttgatttgcg cctacaacgt agaaaaatat tttgcccaat
     2581 cgctgtccgc cgtagtgggg cagacttggc gcaacttgga tattttgatt gtcgatgacg
     2641 gctcgacgga cggcacgccc gccattgccc ggcgtttcca agaacaggac ggcaggatca
     2701 ggataatttc caatccccgc aatttgggct ttatc
//
LOCUS       DAWTQS010000116         2643 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.116, whole genome shotgun sequence.
ACCESSION   DAWTQS010000116 DAWTQS010000000
VERSION     DAWTQS010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.116"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(137..526)
                     /locus_tag="WNA25_002069"
     CDS             complement(137..526)
                     /locus_tag="WNA25_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFA9824729.1"
                     /translation="MNPPEKLLTAENPALRQRAKAMRQEMSEAEAKLWQHLRAGRLNG
                     YKFRRQQPMGNYIVDFMCVTPKLIVEADGGQHAEQAVYDHARTVYLNSLGFTVLRFWN
                     HEILQQTNDVLAEILRVLQELEKQPAR"
     gene            complement(689..1068)
                     /locus_tag="WNA25_002070"
                     /pseudo
     CDS             complement(689..1068)
                     /locus_tag="WNA25_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687733.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1122..2255)
                     /gene="carA"
                     /locus_tag="WNA25_002071"
     CDS             complement(1122..2255)
                     /gene="carA"
                     /locus_tag="WNA25_002071"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFA9824730.1"
                     /translation="MSTPALLVLADGSVFHGTSIGYEGSASGEVVFNTSMTGYQEILT
                     DPSYCKQIVTLTYPHIGNTGTNAEDEESRSVYAAGLIIRDLPLLHSSFRASESLHDYL
                     VRNETVAIADIDTRRLTMLLREKGAQGGAILTGADATVEKAQELIAAFGSMVGKDLAK
                     EVSCTETYEWTEGEWELGKGFVTPDKQPYHVVAYDFGVKTNILRMLASRGCRLTVVPA
                     QTSAEDVLALNPDGVFLSNGPGDPEPCTYAIEAVQKLMESGKPIFGICLGHQLISLAI
                     GAKTLKMRFSHHGANHPVQDLDSGKVVITSQNHGFAVDADTLPANARITHKSLFDNTL
                     QGIELTDKPVFCFQGHPEASPGPQDVGYLFDKFIGNMKAAKQA"
BASE COUNT          543 a          655 c          760 g          685 t
ORIGIN      
        1 tgttggggtt gaggtttgct gcaaggaaaa caggttgtgc aggttgcttt tttgcttttc
       61 agacgacctt tttaaacgct caattcagtt aattttacct tattattttc aaaatctaat
      121 caaaatcaac cgtttgctac cgtgctggct gcttttccaa ttcctgcaat acgcgcagga
      181 tttccgccag tacatcgttt gtctgctgca aaatttcgtg attccaaaaa cgcagcacgg
      241 taaagcccaa gctgttgaga tataccgtcc gcgcgtggtc gtatacggct tgttccgcgt
      301 gctgcccgcc gtctgcttcg acaatcagct tgggcgttac gcacataaaa tcaacaatat
      361 aattccccat cggctgctgt cggcggaatt tatagccgtt cagacggcct gcccgcaggt
      421 gttgccacaa ttttgcttcc gcctcgctca tttcttgacg catggctttg gcgcgttggc
      481 gtagggcggg gttttcggcg gtcaatagtt tctcgggcgg gttcattttt gttatcgggt
      541 tgctgtttgt gtaagcctta cagcttgttg ccctctctct aactctttcc cacagggaga
      601 gaggacgggt cggctgttgg ggttaaaggg gctgcaagga aaacagcttg tgcaggctgt
      661 tttttatttt gcataatccg tctgaaaatt aataactgct gatttccagc aggttgccat
      721 cgggatcgcg caggtaaacc gattggattt tgcccattgc gcctgtgcgc gctacgatgc
      781 cgcttaaagg tttgatgccg tgtgcggata attcctgtaa aaccgtttcc agtggggcgt
      841 atcggtcagc aggcataaat ccgctgtgcc gcaggcggcg tgttgcgcgt taggctgaat
      901 ttccgcaccg cgcccgtgta ggttgatttt ctgactgcca aacaacaaag ctttacggtt
      961 gttgccaaat gaaacttctt ccatgcccaa aacttgtgtg taaaacgcga tggttcggtc
     1021 aatgtcggca acagtcagta ctagatggtc gagtgcgcta attttcattt ccagctttct
     1081 ttttcagacg gcagcagcat actgttgccg tctgaaaatc attatgcttg ttttgcagcc
     1141 ttcatattgc caatgaattt gtcgaacaaa tagccgacat cttgcggacc cgggctggct
     1201 tcggggtgtc cttggaaaca gaacacgggt ttgtcggtca gctcgatgcc ttgcaaggta
     1261 ttgtcgaaca aggatttgtg ggtaatgcgt gcgttggcgg gcagggtgtc ggcatcgacg
     1321 gcgaaaccgt ggttttggct ggtaatgacg actttgccgc tgtccaaatc ttgcacagga
     1381 tggttcgcac cgtggtggct gaagcgcatt ttcagggttt tcgcgccgat ggcgaggctg
     1441 atgagctggt gtcccaagca gatgccgaaa atcggtttgc cgctttccat cagtttttgc
     1501 acggcttcga tggcgtaggt gcaaggctcg gggtcgccgg ggccgttgga caggaacacg
     1561 ccgtcgggat tgagtgccaa cacgtcttcc gcgctcgttt gtgcggggac gacggtcagg
     1621 cggcagccgc gcgaggcgag catacgcagg atgttggttt tcacgccgaa atcgtaggcg
     1681 acgacgtggt aaggctgttt gtcaggggta acgaaacctt tgcccaattc ccattcgcct
     1741 tccgtccatt cgtaagtttc cgtgcaggaa acttcttttg ccaaatcttt gccgaccatg
     1801 ctgccgaacg cggcgatgag ttcttgcgct ttttcaacgg tggcatccgc acctgtcaga
     1861 atcgcgccgc cttgcgcgcc tttttcgcgc aacagcatgg tcaggcggcg ggtgtcgatg
     1921 tcggcgatgg cgacggtttc gttgcgtacc aaatagtcgt gcaggctttc ggaggcgcgg
     1981 aagctgctgt gcaagagcgg caggtcgcgg ataatcaggc cggcggcata aacgctgcgg
     2041 ctttcttcat cttcggcgtt ggtgccggtg ttgccgatgt gggggtaggt gagggtaacg
     2101 atttgtttgc agtaggacgg gtcggtcaga atttcctgat agccggtcat cgaagtattg
     2161 aacacgactt cgccggaagc cgaaccttcg taaccgattg atgtgccgtg gaatacgctg
     2221 ccgtcagcga ggacgaggag ggcgggggtg ctcatgatgg gaatcctgtt tttaataaaa
     2281 ttcttgacaa tgccgtctga agggacttcg gcagggggca tccggtcgga aaaatccggt
     2341 caaaaaaaaa cacgccgcgc aagatagacg cgtaacgtgc ttttcgggca agtgcctata
     2401 ccctgaaagc agcatatttt aagacgaaac atacccgatt tcaagcgcaa acgcggcagg
     2461 cgggctgata tgttcgcgcg aaaacggaaa tccggggcat aaaaccgccc gctccgcgcc
     2521 ccctatgccc gttacgcatt tccccacgtc cgcccggcga aactatggga atacccgaac
     2581 cgtcattccc gcgcaggcgg gaatccggac ctgtcggcac ggaaacttat cgggtacaac
     2641 ggt
//
LOCUS       DAWTQS010000117         2634 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.117, whole genome shotgun sequence.
ACCESSION   DAWTQS010000117 DAWTQS010000000
VERSION     DAWTQS010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.117"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            <1..146
                     /locus_tag="WNA25_002072"
     CDS             <1..146
                     /locus_tag="WNA25_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824731.1"
                     /translation="LRLLQQTACRFKAALETAAWKHYVRAIKESEPVPDAEARRKRKK
                     QAA"
     gene            172..366
                     /locus_tag="WNA25_002073"
     CDS             172..366
                     /locus_tag="WNA25_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824732.1"
                     /translation="MDSEYLDPQQCADILSVKKRTFLERYAPRPDFPARISVSKKRFW
                     WKKEEVEGWLDRQKEKRPMM"
     gene            complement(422..506)
                     /locus_tag="WNA25_002074"
     tRNA            complement(422..506)
                     /locus_tag="WNA25_002074"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(470..472),aa:Leu,seq:tag)
     gene            657..1331
                     /locus_tag="WNA25_002075"
     CDS             657..1331
                     /locus_tag="WNA25_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824733.1"
                     /translation="MPAALIKDFLLTQGLKLPLDEVRAAYLTAQTVMDMGMASIDRSV
                     LWCNDEGWKLADYLPCDDVREDALKRLFMALDSVFSRSTGVRSAAVYALMPSENAALR
                     LVCLSQQGEGLENIWEQDGNITDVSLACRSAQSGWMNVASDVRRWLNLGELSGERNHA
                     SAAQISIPVCTESGGVLGVVHVEFECAECADTAAQAEWVALALALSEPLKQLLGITAA
                     EGDENV"
     gene            1324..1917
                     /gene="ribA"
                     /locus_tag="WNA25_002076"
     CDS             1324..1917
                     /gene="ribA"
                     /locus_tag="WNA25_002076"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFA9824734.1"
                     /translation="MSELLDHVASCRLPTEWGVFTMHGFEEANGQEHVALTVGNCSDG
                     NPVLTRIHSECLTGDALFSRKCDCGPQLEAAMRAVQAEGRGIIVYLRQEGRGIGLINK
                     IRAYHLQEQGMDTVEANLALGLPVDARDFRLAQSIYEYLGIRSVKLLTNNPEKIQTLK
                     DAGINVVERIPLHVGENLENERYLQTKADKLGHLMSE"
     gene            2085..2267
                     /locus_tag="WNA25_002077"
                     /pseudo
     CDS             2085..2267
                     /locus_tag="WNA25_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239093.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            2270..>2634
                     /locus_tag="WNA25_002078"
     CDS             2270..>2634
                     /locus_tag="WNA25_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFA9824735.1"
                     /translation="MRNAVGLDISKLTFDATAIVGNAEYSAKFDNDSKGLNQFSDRLK
                     SLGCQNLHICMEATGNYYEEVADYFAQYYSVYVVNPLKISKYAESRFKRTKTGKQDAK
                     LIAQYCRSAQESELVKRQKP"
BASE COUNT          669 a          594 c          743 g          628 t
ORIGIN      
        1 aactgcgcct attgcaacaa accgcctgcc gcttcaaagc cgccctcgaa acggccgcgt
       61 ggaaacacta cgtccgcgcc atcaaagaat ccgagccggt accggatgcc gaagcgcgcc
      121 ggaagcggaa aaaacaggcg gcgtaaatat aattaagttt ggaggtttta aatggatagc
      181 gaatacctag acccgcagca atgcgcagat attttatccg taaaaaagcg tacattttta
      241 gagcggtacg caccgcgtcc ggatttcccc gcgcgaatat ccgtatcgaa gaaacgtttt
      301 tggtggaaaa aagaagaagt cgaaggatgg ctagaccgcc aaaaagaaaa acgcccgatg
      361 atgtgatttg caccacattt gcaccaagtg gtatataaca ttatgaaaga tataaaaatt
      421 ttggtgcgga cggagagact cgaactctca cacctctcgg cgccagaacc taaatctggt
      481 gcgtctacca atttcgccac gtccgcataa gggtggagat tatacagatt ttgtccgatt
      541 gcgcaaggct ttggacgtaa tagttgaggc ttatgctttg cagcggtaaa atccgctatt
      601 cgtccgcctg gcatcggaat cgggcggttt tttgttttta ttgacggaat ttgggtatgc
      661 ctgctgcttt gattaaggat tttctgctga ctcagggttt gaagctgccg cttgacgagg
      721 ttcgggcggc gtatctgacg gcgcagacgg taatggatat ggggatggct tcgattgacc
      781 gttctgtttt gtggtgcaat gatgagggtt ggaaacttgc cgattacctg ccgtgcgatg
      841 atgtccgcga agatgccctg aaacggcttt ttatggcttt ggattcggtg ttttcacgct
      901 cgacaggtgt gcggagcgcg gcggtttatg ccttgatgcc gtctgaaaac gccgccctcc
      961 ggctggtgtg cctgtcccaa cagggcgaag gtttggaaaa catatgggag caggatggaa
     1021 atattaccga tgtttcgctt gcctgccgtt cggcgcaaag cggttggatg aatgttgcct
     1081 cggatgtacg ccgttggttg aatttgggtg agctttcggg agaacgcaat catgcttcgg
     1141 cggcgcaaat ttccattccc gtttgcacgg agagcggcgg tgtattgggc gtggttcatg
     1201 tggaatttga atgcgcagag tgtgcggata cggcggcgca ggcggaatgg gtggctcttg
     1261 ccttggcttt gtccgaacct ttgaagcaac ttttgggcat cactgccgca gaaggagatg
     1321 aaaatgtctg aattattaga ccatgtcgct tcctgccgtc tgccgaccga atggggcgta
     1381 tttacgatgc acggttttga agaggcaaac gggcaggaac acgtcgcgct gaccgtcggc
     1441 aattgttcag acggcaatcc ggtgttgacg cgcatccact ccgaatgtct gacgggcgac
     1501 gcgctgttct cgagaaaatg cgactgcgga ccgcaacttg aagcggcaat gagggcggta
     1561 caggcagagg ggcgcggcat catcgtctat ctgcgtcagg aaggacgcgg catcgggctg
     1621 attaacaaaa tccgcgccta tcatctgcaa gaacaaggta tggataccgt tgaagccaat
     1681 ttggcactcg ggctgcccgt cgatgcccgc gatttccgtt tggcgcaatc tatctacgaa
     1741 tatctgggca tccgctcggt caaactgttg accaacaacc ccgaaaaaat ccaaaccctg
     1801 aaagatgcgg ggattaacgt ggtcgaacgc attcccctgc acgtcgggga aaatctggaa
     1861 aacgagcgtt atctccaaac caaagcagac aagctggggc atttgatgtc ggaataaggc
     1921 aaagttgcag ggaacgggca tcctgcgccg tctttcggga aacaggtttc catagcttga
     1981 taaagcaata agttttatca agttgcaggg tgcggatgca aacgcattgc gagcgcgggt
     2041 ttgaggcata cgcgcaaaca tcttaataca acgtattgat atttatgatt ttctctatca
     2101 tcgtccctat ttacaatgtg gaaaaatacc ttcgctgctg cgtggattcc gtgcttgccg
     2161 aaaattttgc cgattatgaa atgattttgg tcgatgacgg ttcgccggac ggctgcggga
     2221 agatttgcga cgaatatgca ggcaaatatc cgcatataag ggtaatccca tgcgtaacgc
     2281 cgtaggattg gacatatcca agctgacatt tgacgcaacg gccattgtcg gcaatgccga
     2341 atattcggca aagtttgaca acgattcaaa aggtttaaat cagttttcgg accggttgaa
     2401 aagcttggga tgtcagaatc tgcatatctg catggaggca accggcaatt attatgaaga
     2461 agttgccgac tacttcgcgc agtattacag cgtttacgta gtgaatccgc tgaaaataag
     2521 caagtatgca gaaagcaggt tcaagcgaac caaaacaggc aaacaggatg caaagctgat
     2581 agcgcagtat tgccggtcgg cgcaggaaag cgagcttgta aagaggcaga agcc
//
LOCUS       DAWTQS010000118         2609 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.118, whole genome shotgun sequence.
ACCESSION   DAWTQS010000118 DAWTQS010000000
VERSION     DAWTQS010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.118"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(46..705)
                     /locus_tag="WNA25_002079"
     CDS             complement(46..705)
                     /locus_tag="WNA25_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFA9824736.1"
                     /translation="MTPPKLIIFDWDGTLADTTQPIIDTMRRSFAECGFPPPEAERVR
                     SLIGYSLPEIIRALLEMPSEAAVADIARTYSAHYLNPNNRNMTLFPDAPPCLDKLKAQ
                     GFRLAVATGKGRAGLDNAISQTATGGYWLATACAGEYPSKPSPEMVFGLCGELGLDPK
                     EALVVGDTAHDLHMAANAGAAAVGVATGAHSREQLLGAPHLAVLDGLSELPGFLARHY
                     A"
     gene            865..1080
                     /locus_tag="WNA25_002080"
     CDS             865..1080
                     /locus_tag="WNA25_002080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824737.1"
                     /translation="MSVFRINMTAATVLAALSSPVFAAQTADLETVHIKGQRSYNAIA
                     TEKNGDYSSLKAAIWMTASIPASPPAA"
     gene            1026..1569
                     /locus_tag="WNA25_002081"
                     /pseudo
     CDS             1026..1569
                     /locus_tag="WNA25_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467916.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            1639..1959
                     /locus_tag="WNA25_002082"
     CDS             1639..1959
                     /locus_tag="WNA25_002082"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824738.1"
                     /translation="MRSQPEYGIAKENRPNRQADRFAGSVLPLAASLTHKPNIMKPIP
                     TDTFQPAILPQAFETEIKSTCTGRIYRIQTATLGEMQSEGYPVLFVLDGEAFFPRCTT
                     SCSR"
     gene            1947..2569
                     /locus_tag="WNA25_002083"
                     /pseudo
     CDS             1947..2569
                     /locus_tag="WNA25_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950989.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
BASE COUNT          603 a          757 c          734 g          515 t
ORIGIN      
        1 aggcttcaga cggcattttt gccgcgtgcc ggatgcggaa accaatcagg cgtaatgtcg
       61 tgcaagaaaa ccgggcagtt cggacaaacc gtccaatacg gcgagatgcg gtgcgccaag
      121 gagctgttcg cgcgaatgtg cgccggtggc aacgccgact gccgccgcgc ctgcgtttgc
      181 cgccatatgc aggtcgtgcg ccgtatcgcc gacaaccaat gcctctttcg ggtcgagtcc
      241 cagttcgccg cagagtccga ataccatttc gggcgagggt ttggaggggt attcccccgc
      301 gcaggcggtg gcgagccaat agccgccggt ggcggtttgg ctgatggcgt tgtccaaacc
      361 cgcccgccct ttgcccgtgg cgacggcaag ccggaagcct tgcgctttga gcttgtccag
      421 acagggcggg gcatcgggaa agagtgtcat attgcggttg ttgggattga ggtaatgcgc
      481 ggaataagtg cgcgcgatgt cggcaacggc agcttcagaa ggcatttcga gcagggcgcg
      541 gatgatttcg ggcaggctgt atccgatcag gctgcggacg cgctccgctt cgggcggcgg
      601 aaaaccgcat tcggcgaagc tgcggcgcat ggtgtcgatg atgggttggg tcgtatcggc
      661 aagcgtgccg tcccagtcga agatgatgag tttgggcggg gtcatggcag gttggttgca
      721 gtaaaaaagc agattttatg cggaaaacgc agacgtgtcg cattttcgac aaaatttgtc
      781 ggctgagcga tatgtttttc cgaacaagcc gcgttgtgct ttattaaaat agaaccatta
      841 tcatttatac agatgggaca gtttatgtca gttttccgca tcaatatgac cgccgccacg
      901 gttttggcag cactctcgtc tccggttttt gccgcacaaa cggcggattt ggaaaccgtc
      961 catatcaaag ggcagcgttc gtacaacgcg attgccaccg agaaaaacgg cgattacagc
     1021 tcgttaaagg cagctatatg gatgaccgcc tcaatacccg cgtctccccc tgccgcctga
     1081 aagacaaaaa cgccgccgaa cccgaacaac cgcaacaccc gttacgccgc attgggcaaa
     1141 cgcgtgatgg aaggcgttga gaccgaaatc agcggtgcga ttacaccgaa atggcaaatc
     1201 catgcaggtt acagctatct gcacagccaa atcaaaaccg ccgccaatcc acgcgacgac
     1261 ggcatcttcc tgctggtgcc caaacacagc gcaaacctgt ggacgactta ccaagttacg
     1321 cccgggctga ccgtcggcgg cggcgtgaac gcgatgagcg gcattacttc atctgcaggg
     1381 atgcatgcag gcggttatgc cacgttcgat gcgatggcgg cataccgctt cacgcccaag
     1441 ctgaagctgc aaatcaatgc cgacaacatc ttcaaccgcc attactacgc ccgcgtcggc
     1501 ggcacgaaca cctttaacat tcccggttcg gagcgcagcc tgacggcaaa cctgcgttac
     1561 agtttttaaa gaccaatatg ccgtctgaaa cggcagccgc agcataatca aaccacaaca
     1621 agctgcgcgg cataccctat gcgctcacaa ccggagtatg gcattgcgaa ggaaaacaga
     1681 ccgaaccggc aggcagaccg ctttgccggt tcggttttac cgcttgccgc cagtctgacc
     1741 cacaagccga acatcatgaa acccataccg accgacacat tccaacctgc catactgccc
     1801 caagcctttg aaaccgaaat caaatccacc tgcacggggc gaatctatcg gattcagacg
     1861 gcaacactcg gcgaaatgca gtctgaaggc tatcccgtcc tctttgtcct cgacggcgaa
     1921 gcctttttcc cgcgctgtac aacatcatgc agtcgctgat gaacaacccc gttacccgaa
     1981 gcaacgcccc ctgcctgatt gtcggtatcg gctacacgac aggcagtgtg cgcgacctgg
     2041 cgcagcgtgc cgccgactac acgccgccgc ttggagacaa cgccacagca gacgaacggc
     2101 ggcagttcgg acaggcagac cgcttcgccg actttatcga cagcgaactg accgcctttt
     2161 tagaaagccg ctacaccctt aaccgtaatg aaaccgccgt attcggacac tcgttcggcg
     2221 cactgttcgg actgtattcc ctgctttccc accgctgttt cagacggcat tggctcgtat
     2281 cccctcgatt tggtggcaca acaggcggat actcgacttt atgccgtctg aaaaccggct
     2341 ggacggcatc gatgcctgcc tcaacatcgg cgcactcgag cggagcagcg gttgcaaacg
     2401 cagggaagaa cgcgacatgg cagggcaggc cgaacaaatg gcggcagagt tggacaggcg
     2461 cgggaccgcc gtatttttcc gggaatatcc gaatgccgac cacggcaatg tcccgtttta
     2521 ctcgctgacc gactgcgtcg aatatttgag ggaagcgtgg caacggtagg gggaatcaaa
     2581 tatatgactg ctttgttttg catcggaaa
//
LOCUS       DAWTQS010000119         2562 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.119, whole genome shotgun sequence.
ACCESSION   DAWTQS010000119 DAWTQS010000000
VERSION     DAWTQS010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.119"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            434..>2562
                     /locus_tag="WNA25_002084"
     CDS             434..>2562
                     /locus_tag="WNA25_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HFA9824739.1"
                     /translation="MNKTLKRRVFRHTALYTAILMFSHTGGGGAMAQTHQYAIIMNER
                     KQPEVKWKDQYNQSALKDKSRERTFSHTSQKNGLGITSNFISFNNNDELVSQQSGTAV
                     FGTATYLPPYGKVSGFDTAELNKRGNAVNWIHTTRPGLAGYGYTGIRCGSTKDCPKLT
                     YKTRFSFDNPDLLKTRGGLDRHTEPSRENSPIYKLKDYPWLGVSFNLGGEGTAKDGRS
                     SSKLVSSFDENNSNSNQNLVYTTEDHRISLSNWQQETTAMAYYLNAKLHLLDKKGIKD
                     ITGKTVRLGVLKPSIDVKTQNTGLSGILNFWSKWDIKDNGQIPVKLGLPEVKAGRCIN
                     EPNPNKNTQAPSPALTAPALWFGPGQNGKVQMYSASVSTYPGSSSSRIFLQELKTQTD
                     PARPGRHSLAALNARDIKSREPNFNSRQTVIRLPGGVYRIGPTRNGIVGLNGNDGKND
                     TFGIYKDRLVTPEVDEWAKVLLPWTVRYYGNDDIFKTFNQPNNKKQSDKKQYSQKYRI
                     RTKEDDNDKPRDLGDIVNSPIVAVGGYLATSANDGMVHLFKRNGTNQRGYELKLSYIP
                     GTMERKDIENQDSTLAKELRAFAEKGYVGDRYGVDGGFVLRRITDDQDREKHFFMFGA
                     MGLGGRGAYALDLTKIDSNNLTGVSMFDVQNDKNNNNNDSNRVKLGYTVGTPQIGKTQ
                     NGKYAAFLASGYAAKDIVSSDNTTALYV"
BASE COUNT          705 a          704 c          660 g          493 t
ORIGIN      
        1 aatgccgcct ccccggcccg ccattgccgc gcaggcggca atggcgggcc gggcaaacgg
       61 ttgcggattg ccgaaaaccg ctttaccatg atcagcgcgc aagggtagaa taatgcggta
      121 accctataca ttgcatccgg ccaaaggggc gtgttacctt tgcgaacatc cccctttggc
      181 agccgggcga agggggcttt gcaaccggca atccggcggg cgcgggatcg ggcggtttgc
      241 ccaatcccgc cgtttgcggc gcgcctgccg ccgacggtat cccgcgaagc aagatttaag
      301 ggataaaata tgttccaaca cgcagggcgg cacataaggc gccgccctga ttcggaaggg
      361 cttgcacccc tcccggacaa agcctgatcc tgccgccccg aagggcgggg ggtttgaccg
      421 aaaaggaaat acgatgaata aaactttaaa aaggcgggtt ttccgccata ccgcgcttta
      481 taccgccatc ttgatgtttt cccataccgg cggggggggg gcgatggcgc aaacccatca
      541 atacgctatt atcatgaacg agcgaaagca gcccgaggta aagtggaagg atcaatataa
      601 tcaatcagca ttaaaggaca aaagcaggga gcggacattt agccatacga gccagaaaaa
      661 cggcctcggc atcacaagca attttatctc attcaacaat aacgatgagc ttgtttctca
      721 acaaagcggt actgccgttt ttggcacagc cacctacctg ccgccctacg gcaaggtttc
      781 cggctttgat accgccgagc tgaacaagcg cggcaatgcc gtcaattgga ttcataccac
      841 ccggcccggg ctggcaggct acggctacac cggtatccgt tgcggaagta ccaaagactg
      901 tcccaaactt acctataaaa cccgattttc cttcgataat cccgacttgc taaaaacaag
      961 aggcgggctg gataggcaca cagagccaag ccgcgaaaat tcgcccattt acaaattgaa
     1021 ggattatcca tggttgggtg tgtctttcaa tttgggcggc gagggtaccg ccaaagatgg
     1081 cagatcatcc agcaaattgg tatcttcttt tgatgaaaac aatagtaata gtaatcaaaa
     1141 cctcgtctat acgacagaag accaccgtat ttcccttagc aactggcagc aagaaacgac
     1201 cgccatggcc tattatctga acgccaagct gcacctgctg gacaaaaaag ggattaaaga
     1261 tatcaccggc aaaacagtgc ggttgggtgt cttgaagccg agcatcgatg tgaagacaca
     1321 aaatacgggg cttagcggca ttctaaattt ttggtctaag tgggacatta aagataacgg
     1381 gcagattccg gtcaagctcg gcctgccgga agtcaaagca ggccgctgca tcaacgaacc
     1441 gaaccccaat aagaataccc aagccccttc gccggcactg accgcccccg cgctgtggtt
     1501 cggccctggg caaaatggta aggtgcagat gtattccgct tcggtttcta cctaccccgg
     1561 cagttcgagc agccgcatct tcctccaaga gctgaaaact caaaccgacc ccgcccggcc
     1621 cggccggcat tccctcgccg ctttgaatgc gcgggatatc aaatcccgcg agccgaattt
     1681 caactcaagg cagaccgtga tccgattgcc gggcggcgtg taccggatcg gcccgactcg
     1741 caacgggatc gtgggtttga atggcaatga cggcaaaaac gacactttcg gcatctacaa
     1801 ggacaggctc gtcacacctg aggtcgacga gtgggcaaaa gtgctgctgc cttggacggt
     1861 ccggtattac ggtaatgacg atatatttaa aacattcaac caaccaaaca acaaaaaaca
     1921 aagcgacaaa aaacaataca gccaaaaata ccgcatccgc acaaaagaag atgacaatga
     1981 caaaccccgc gatttgggcg acatcgtcaa cagcccgatt gtcgcggtcg gcgggtatct
     2041 ggcaacttct gccaacgacg ggatggtgca cctgtttaaa agaaacggca caaaccaacg
     2101 aggctacgaa ctgaagctca gctacatccc cggtacgatg gagcgtaagg atattgaaaa
     2161 ccaagactcc accctcgcca aagagctgcg cgcctttgcc gaaaaaggct atgtgggcga
     2221 ccgctacggc gtggacggcg gctttgtctt gcgccgcatt acagatgacc aggacaggga
     2281 aaaacatttc tttatgtttg gtgcgatggg ccttggcggc agaggcgcat acgccttgga
     2341 tttgaccaaa atcgacagca acaacctgac cggcgtttcc atgtttgatg tccaaaacga
     2401 caaaaataac aataacaatg acagtaatcg cgtgaaatta ggctacaccg tcggtacgcc
     2461 gcaaatcggc aaaacccaaa acggcaaata cgccgccttc ctcgcctccg gttatgcggc
     2521 taaagatatt gtcagcagcg ataatacaac cgcgctgtat gt
//
LOCUS       DAWTQS010000120         2558 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.120, whole genome shotgun sequence.
ACCESSION   DAWTQS010000120 DAWTQS010000000
VERSION     DAWTQS010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.120"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            <1..245
                     /locus_tag="WNA25_002085"
     CDS             <1..245
                     /locus_tag="WNA25_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824740.1"
                     /translation="VLAAVLAAGYAFGFAKGGGSRTAKGKPAGIAAMRMALAQKQAEV
                     DELSAEIWLEERHLNAGEEAGCRRVHGDAEVPEGKE"
     gene            242..403
                     /locus_tag="WNA25_002086"
     CDS             242..403
                     /locus_tag="WNA25_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824741.1"
                     /translation="MSFHPETAYTGGGETEPYGPSPEEIKYRQSPETAETRRMTEKQA
                     EGHIKSIIR"
     gene            472..1158
                     /locus_tag="WNA25_002087"
     CDS             472..1158
                     /locus_tag="WNA25_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824742.1"
                     /translation="MSLILSVKDESNFKPCPAGSHHATCIRIIDLGTQLVEYQNEQKR
                     QHKILVQWEIDPEGDPEMLMPDGRPYLISRRYTASLHSKSQLATDLKSWRGRDFTPEE
                     RDNFDLRNILGKPCLLSIAHQESSDGKTTYANISAISNKMKSYTPKQPDNAVFAFDLS
                     DPDWANYGLLNEKLREQIAKSPEYAEAVNGRQPPAPPQKQAQAAEGRTEYPQGNAAPA
                     EDIEDDIPFN"
     gene            1298..1480
                     /locus_tag="WNA25_002088"
     CDS             1298..1480
                     /locus_tag="WNA25_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824743.1"
                     /translation="MANIDLTQWDGKTIGAAANPEQGYINITIGSDDLFINIEQAYAI
                     HAALGEAVAEYEGGAQ"
     gene            1477..1968
                     /locus_tag="WNA25_002089"
     CDS             1477..1968
                     /locus_tag="WNA25_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HFA9824744.1"
                     /translation="MTALALYRCAADVQAALDYYFDSETEREDTLEAVIGQFEVKAQS
                     VIAYIKNQEITEKMLEGHIRQMTGKLKAAKARNQSLKDYLARNMQAAGITEIKADDGT
                     FKASFRKSEAVVILDEAQIPAEFMREAVKTEPDKTAIRKAIESGRQVAGAKIEGRKNL
                     QIR"
     gene            2020..2235
                     /locus_tag="WNA25_002090"
     CDS             2020..2235
                     /locus_tag="WNA25_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824745.1"
                     /translation="MSYLEDVKNALRVIDNLCKEALKEPESLEGYIDEIRDKADEADT
                     SLEFLKDVINYGISDLKNVIEVFEDCV"
     gene            2346..>2558
                     /locus_tag="WNA25_002091"
     CDS             2346..>2558
                     /locus_tag="WNA25_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824746.1"
                     /translation="MQTVATKPTAKQMLAAKRAAKESTRQERAVKRAGTVKNVDRNRL
                     SARSKAQKENIARMLSGAKVSEDEALT"
BASE COUNT          803 a          648 c          705 g          402 t
ORIGIN      
        1 cggtattggc ggcggtattg gcggcgggct atgccttcgg attcgccaag ggaggcggaa
       61 gccgtacggc aaagggaaaa cccgccggta ttgcggcaat gcgcatggcg ttggctcaaa
      121 agcaggcgga agttgacgaa ttgagcgccg agatctggct ggaagagcgg catctgaatg
      181 cgggggaaga agccggttgc cggcgggtgc acggcgatgc ggaagtgccg gagggtaaag
      241 aatgagcttc catcccgaaa ccgcttacac cggcggcgga gaaacagagc cgtacggacc
      301 aagccccgaa gaaatcaaat accggcaaag cccggaaacc gccgaaacac ggcggatgac
      361 cgaaaaacag gcagaaggcc acattaaaag cattatcagg taacgccgcc gcgcaaggca
      421 taaaacagcg cgagaccgat agacgaaacg acctttaaca ggagaccgaa aatgtcattg
      481 attttaagtg tgaaagacga aagcaatttc aaaccatgcc cggcaggcag ccatcacgcc
      541 acctgcatcc gcatcatcga tttgggtacg cagctcgtcg agtaccaaaa cgaacaaaag
      601 cggcagcaca agattttagt gcagtgggaa atcgacccgg aaggcgatcc ggaaatgctg
      661 atgccggacg gcaggcccta cctcatcagc cgccggtaca ccgccagcct gcacagcaaa
      721 agccaactgg caacagacct caaaagctgg cgcggaaggg actttacacc ggaagaacgc
      781 gacaacttcg atttgcgcaa tattttgggc aagccctgcc tgttgagtat cgcccaccaa
      841 gaaagcagcg acggcaaaac cacctatgcc aacatttccg ccatcagcaa caagatgaag
      901 agctacaccc cgaaacagcc ggacaacgcc gtttttgcct tcgacctgtc agaccccgat
      961 tgggcgaatt acggcctcct gaacgagaaa ctgagggagc agattgccaa aagcccggaa
     1021 tatgccgaag ccgtaaacgg ccgccaaccg ccggcgccgc cgcagaaaca ggcccaagcg
     1081 gcggaagggc ggacggaata tccgcaaggc aatgccgcgc ctgccgaaga catcgaggac
     1141 gacatccctt ttaattaagc ccgccgtcag ggcgtaaaac cggacggaaa accgcaaaca
     1201 aacgccgcgc cctgcgggcg ggccccgcac tctgaagcag tagtcgattt ttagtttgca
     1261 actgcttcgg acggcaacag agaaaggaaa caaaaaaatg gcaaacatcg acctgaccca
     1321 atgggacggg aaaaccattg gcgccgccgc caatccagaa cagggataca tcaacatcac
     1381 catcggcagc gacgacctat tcatcaacat cgaacaggca tacgccatac acgccgcgct
     1441 tggcgaagcg gttgccgaat atgagggagg ggcacaatga ccgccctcgc actctaccgg
     1501 tgcgcggcag acgtacaggc ggcgcttgat tactacttcg acagcgaaac cgagcgcgaa
     1561 gacacgctgg aagccgttat cgggcagttc gaggtcaaag cgcaatccgt tatcgcttat
     1621 attaaaaacc aagaaatcac ggaaaaaatg cttgaagggc acatcaggca gatgaccggg
     1681 aagctcaagg cggcaaaagc gcggaatcaa agcctgaaag actacttggc gcgcaatatg
     1741 caggcggcgg gcattaccga aatcaaagcg gacgacggca cttttaaagc ctcgttccgc
     1801 aaatccgaag ccgtcgtgat cttagacgaa gcacaaatcc ccgccgaatt tatgcgtgag
     1861 gccgtcaaaa ccgaaccgga caaaaccgcc atcagaaaag cgattgaaag cggtcggcaa
     1921 gtagcaggcg cgaagattga agggcggaag aatttgcaga ttagataaac cgtagaagat
     1981 gttgacgaca gcatcccatt ttgagttaag gagcaaaaaa tgagctattt ggaagatgta
     2041 aaaaacgcat taagggtaat agataactta tgcaaagaag cattaaaaga gcctgaatcg
     2101 ttagagggtt atatagacga aattagggat aaagcagacg aagcggatac ctctttggaa
     2161 tttctaaagg atgtaataaa ttatggtatt agcgatttaa aaaatgtaat tgaggtgttt
     2221 gaagattgcg tttgatatta atggaacaag acatgaacta tcaatttaaa ttcggcgacc
     2281 ccgtgagaga tacagcttta tgattgggca ttgagagaac ggcatcaaaa aatcgagaaa
     2341 acaaaatgca aacagtagca acaaaaccga cggcaaaaca gatgcttgcc gccaaacgcg
     2401 cggcgaagga atcaacgcgg caggaacgcg ccgtcaaacg cgcgggaaca gtaaaaaacg
     2461 ttgaccggaa ccggctgtcc gcccggtcaa aagcgcaaaa agaaaacatc gcccggatgt
     2521 tgtcgggcgc aaaggtatcg gaagacgaag ccctgacg
//
LOCUS       DAWTQS010000121         2345 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.121, whole genome shotgun sequence.
ACCESSION   DAWTQS010000121 DAWTQS010000000
VERSION     DAWTQS010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.121"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            <1..531
                     /locus_tag="WNA25_002092"
     CDS             <1..531
                     /locus_tag="WNA25_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HFA9824747.1"
                     /translation="FGGWRVAADYARYRKWNNNKYSVNIEKVQEVGKNRRDRKTENQE
                     NGSFHAVSSLGLSAVYDFRPNDKFKPYIGVRVAYGHVRHGIDSTKKITGTLTAYPNDA
                     DAAATVYPDGHPQKNTYQKSNSSRRLGFGAMAGVGIDVAPGLTLDAGYRYHNWGRLEN
                     TRFKTHEASLGVRYRF"
     gene            573..899
                     /locus_tag="WNA25_002093"
     CDS             573..899
                     /locus_tag="WNA25_002093"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824748.1"
                     /translation="MTRSPFTGAGQVRGNTRTAIADNTAISKPVIPAQAGIRICRDSL
                     KSGGGGMVLQINDVDTNSGEFAIYTAQDASVKLDLLKMELKHCKEMLEQKDKEIELLR
                     KLTETV"
     gene            988..1557
                     /gene="rsmD"
                     /locus_tag="WNA25_002094"
     CDS             988..1557
                     /gene="rsmD"
                     /locus_tag="WNA25_002094"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFA9824749.1"
                     /translation="MAAGKHTKHSNRVRIIGGQCRGRKLSFASADGLRPTPDSVREKL
                     FNWLGQDLTGKTVLDLFGGSGALGMEAASRNAKRVVIADNNRQTVQTLEKNSRELGLG
                     QVQTVCSDGIAYLANLKEKFDVVFLDPPFAWQSWESLFNVLGTRLNDGAYVYIEAGRQ
                     PDKPDWLTGYREGKSGQGTFELRVFQVAE"
     gene            1680..1931
                     /locus_tag="WNA25_002095"
     CDS             1680..1931
                     /locus_tag="WNA25_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HFA9824750.1"
                     /translation="MSLFITDECINCDVCEPECPNDAISQGEEIYEINPNLCTQCVGH
                     YDEPQCQQVCPVDCILIDEEHPETHDELMAKYEKIIQFK"
     gene            2028..2111
                     /locus_tag="WNA25_002096"
     tRNA            2028..2111
                     /locus_tag="WNA25_002096"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2062..2064,aa:Tyr,seq:gta)
     gene            2142..2215
                     /locus_tag="WNA25_002097"
     tRNA            2142..2215
                     /locus_tag="WNA25_002097"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2174..2176,aa:Gly,seq:tcc)
     gene            2225..2299
                     /locus_tag="WNA25_002098"
     tRNA            2225..2299
                     /locus_tag="WNA25_002098"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2257..2259,aa:Thr,seq:ggt)
BASE COUNT          645 a          570 c          608 g          522 t
ORIGIN      
        1 ttcggcggct ggcgcgtcgc cgcagattat gcccgttaca gaaagtggaa caacaataaa
       61 tattccgtca acatagaaaa ggtgcaagaa gtcggtaaaa acaggagaga ccggaagacg
      121 gaaaatcagg aaaacggcag cttccacgcc gtttcttctc tcggcttgtc cgccgtttac
      181 gatttcagac ccaacgataa attcaaaccc tatatcggtg tgcgcgtcgc ctacggacac
      241 gtcagacacg gcatcgattc gactaaaaaa ataacaggta ctcttaccgc ctaccctaat
      301 gatgctgacg cagcagctac ggtttatcct gacggacatc cgcaaaaaaa cacctatcaa
      361 aaaagcaaca gcagccgccg cttgggcttc ggcgcgatgg cgggcgtggg catagacgtc
      421 gcgcccggcc tgaccctgga cgccggctac cgctaccaca actggggacg cttggaaaac
      481 acccgcttca aaacccacga agcctcattg ggcgtgcgct accgcttctg attccccgac
      541 actgatgccg tctgaacctt cagacggcat ttttgacgcg ctcgccgttt acgggcgcgg
      601 ggcaggtgcg gggaaatacc cgtaccgcca ttgccgacaa caccgcaatc tcgaaacccg
      661 tcattcccgc gcaggcggga atccggattt gtcgggattc gctcaaatcg ggcggcggcg
      721 ggatggtgtt gcagattaac gatgtggata ccaacagcgg ggaatttgca atctataccg
      781 ctcaggatgc atcggtaaag ctggatttgt taaaaatgga attaaaacac tgcaaagaaa
      841 tgttggaaca aaaagacaaa gaaatcgagt tgctccgcaa gctgaccgaa accgtttaaa
      901 cagatatgcc gtctgaaaaa agttttcagg cggcatattc ttcgacaggt cttgtataat
      961 accgtttgaa ctttcaggct tttgattatg gcggcaggca aacataccaa acacagcaac
     1021 cgggtacgca ttatcggcgg gcaatgccgg ggcaggaaat tgagttttgc atccgccgac
     1081 ggactgcgcc caacccccga cagcgtgcgt gaaaagctgt ttaactggct gggacaggat
     1141 ttgacgggaa aaacggtttt ggatctcttc ggaggcagcg gcgcactcgg catggaggcg
     1201 gcatcgcgta atgcaaaacg ggtcgtgatt gcagacaaca accgtcaaac ggtgcaaacc
     1261 cttgagaaaa acagccgcga actgggtttg gggcaggtgc aaaccgtctg ttcggacggc
     1321 atcgcatatt tggcaaacct aaaagagaaa ttcgatgtcg tcttccttga tccgccgttt
     1381 gcgtggcaaa gttgggaaag tcttttcaat gtattgggca cacgcctgaa tgacggcgca
     1441 tatgtttata tcgaggcagg caggcagccg gacaaacctg attggctgac gggatatagg
     1501 gaggggaagt cggggcaggg tacatttgaa ttaagggttt tccaagtggc tgaataatat
     1561 gcgctttgat aatcatttcc gggttgtaaa cattcgtttg caaccgtccg gttcaaaaaa
     1621 accttgtgct ataatccgcg cccgcccggt tttgataatt tagtggaaaa ggaaaagaaa
     1681 tgtcgctttt tattaccgac gagtgcatca actgcgacgt atgcgaaccc gaatgcccca
     1741 atgatgccat ttcccaaggc gaggaaattt acgaaatcaa ccccaacctc tgcacgcagt
     1801 gcgtcggaca ctacgatgag ccgcagtgcc agcaggtttg cccggtggac tgcatcctga
     1861 ttgacgaaga acatcccgaa acccatgacg agttgatggc gaaatacgaa aagattatcc
     1921 agtttaaata aattcttttt aaaacatcaa attatgtctg ttttgaaata aaatcaaaaa
     1981 aaaacttgac ggaaaagcaa gccgctaata aactagtgtt ctcttttgga gggattcccg
     2041 agcggtcaaa gggggcagac tgtaaatctg ttgcgaaagc ttcgaaggtt cgaatccttc
     2101 tccctccacc aaaattctta cttggggcag tagcgagtaa tgcgggtgta gctcaatggt
     2161 agagcagaag ccttccaagc ttacggtgag ggttcgattc ccttcacccg ctccaaacaa
     2221 ttaggcccat gtagctcagg ggtagagcac tcccttggta agggagaggt cggcagttca
     2281 aatctgccca tgggcaccat ctctcgatta ttcatttctt taaggcttag atatatagga
     2341 tattg
//
LOCUS       DAWTQS010000122         2265 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.122, whole genome shotgun sequence.
ACCESSION   DAWTQS010000122 DAWTQS010000000
VERSION     DAWTQS010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.122"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            112..336
                     /locus_tag="WNA25_002099"
     CDS             112..336
                     /locus_tag="WNA25_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824751.1"
                     /translation="MFIFYTVNPEHVYFPKAYIMKVFKDKGYESQCITTVSFYICNPT
                     LKQKTENEAYEYGRLFVKELMHKECNRESL"
     gene            323..1651
                     /locus_tag="WNA25_002100"
     CDS             323..1651
                     /locus_tag="WNA25_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFA9824752.1"
                     /translation="MKAFEDKALAVSADMADALADGGAAATAVGCPPRLIGGEQNKTP
                     NPKGAEKTENQEFQFEYFSHFVSDGKGRFIEIPLRRGRDDGAFIDQITFTIHENSMTK
                     VTGKGLVSDTEFVVRYSELLEEILGFGITKKLPFKGKFFYQSCYQFGPDNVEYGKVHY
                     GGQCETMLVELNGTGCMAALPGWENRLYEFLSKCVRPKITRIDVAHDFFNGEYTPDQA
                     MLDHDNGHYDVHNMRPKSECRGTAWRNEDGSGKTFYIGKRGNSKFTRVYEKGRQLGDV
                     DSPWVRFEGDIEIPLDVLLYSGSYLGGAYPICKEIFKTEAKRMEVKVKNVNLIFDVKL
                     FHARNQVGKMVNFLRDIGWDDSRIVDELVKGVEGYPKGLQPEQYDCKNQTQKFQYIHE
                     EQKAINALNIETLFDDLIEEREYAFPQDREWQHIKDIEYEERQISDFLNN"
     gene            1665..1976
                     /locus_tag="WNA25_002101"
     CDS             1665..1976
                     /locus_tag="WNA25_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824753.1"
                     /translation="MFETSQVTTYTATLLGAKKFKGEIDGNKIDSCTVLVASPMPSNG
                     NAVGFTSESMKFGDSHNFEKLKNLKFPCAVDLTVAMESTGKGLVQKLLDFQVKGAAPK
                     A"
     gene            1990..2193
                     /locus_tag="WNA25_002102"
     CDS             1990..2193
                     /locus_tag="WNA25_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824754.1"
                     /translation="MSRYQQKFIVQELENHEFIYPDSFGDIGFTSNIKSAGKYDSYED
                     AFSSALEEIGGEFVIFGFYEKED"
BASE COUNT          736 a          446 c          535 g          548 t
ORIGIN      
        1 ggagcggata aattctcgca atctaaaacc ttatatattg cgctccataa ggggcgcata
       61 tttttaatcc ttgtaaagcc ccttaactct agtcagtgaa ggggcttaat catgtttatt
      121 ttctatactg tcaatcccga acacgtatat ttccccaaag catacatcat gaaagtgttc
      181 aaggataaag gctacgaatc ccaatgcatc acaacagttt ctttttatat ctgcaatccg
      241 actttgaagc aaaagaccga aaacgaagcg tatgaatacg gcagattgtt tgttaaagaa
      301 ctgatgcata aggaatgcaa ccgtgaaagc ctttgaagat aaagccttgg cggtgtccgc
      361 ggacatggcg gacgcgttag cggacggcgg ggctgcggca accgccgtag gctgcccccc
      421 taggctaata gggggggagc aaaataaaac cccaaatccg aagggtgcgg aaaaaacaga
      481 aaaccaagaa tttcaattcg agtatttcag tcattttgta tcggacggca aaggcagatt
      541 tatagaaata ccgttaaggc gtggcaggga tgacggagcg ttcatagacc aaatcacttt
      601 cacgatacat gaaaattcca tgacgaaagt aaccggaaag ggattggtat cagatacgga
      661 attcgttgtc cggtatagcg agctgcttga agaaatcttg ggattcggca ttaccaaaaa
      721 attaccgttt aaaggaaagt ttttctatca aagctgttat caattcgggc ccgataacgt
      781 cgaatacgga aaggttcatt acggcggcca atgtgaaacg atgttggttg aattgaacgg
      841 tacaggctgc atggctgctt tacccggttg ggaaaaccga ttgtatgaat ttttaagtaa
      901 atgcgtccga cccaaaataa cccgtatcga tgtcgcccat gattttttca acggagagta
      961 cacacccgat caggcaatgt tagaccacga taacggacat tacgacgtac acaatatgcg
     1021 gccaaaaagc gaatgtcgcg gcacggcatg gcgcaatgaa gacggcagtg gaaaaacctt
     1081 ttacatcggc aagcgcggaa attcaaaatt tacccgtgtt tacgagaaag gcagacagct
     1141 tggagatgtt gatagccctt gggtcaggtt tgagggggat atagaaatac ccttggatgt
     1201 cctactttat tcggggtcgt atctaggcgg cgcatatccg atttgcaagg agatttttaa
     1261 gacagaagcc aagcgcatgg aagtaaaagt taaaaacgtt aatctgatat tcgacgttaa
     1321 gctttttcat gcaaggaatc aagtcggcaa gatggttaat ttcctccgcg atatagggtg
     1381 ggacgatagc aggatagttg atgagctggt gaaaggcgtt gaaggttatc caaaaggttt
     1441 acagcctgag caatacgatt gcaaaaacca aacgcagaaa ttccagtata tccacgaaga
     1501 acagaaagcc attaacgcat tgaacattga aacactgttt gatgatttga ttgaagagag
     1561 agaatacgca tttcctcaag acagggaatg gcaacacatc aaggatattg agtacgagga
     1621 acgtcaaata tccgattttt taaacaactg aaggaaattg aaaaatgttt gaaacaagcc
     1681 aagtaaccac atataccgcc actttgttgg gcgcaaaaaa attcaaaggc gaaatcgacg
     1741 gtaacaaaat cgattcatgc accgtcttgg tagccagccc catgccgtca aacggcaatg
     1801 ccgtgggctt tacctcggaa agcatgaagt ttggtgacag ccataatttt gaaaaattga
     1861 aaaaccttaa attcccctgt gcggttgatc taaccgttgc catggaatca acaggaaaag
     1921 gcttagtgca gaaattgttg gatttccaag taaagggcgc agcacctaaa gcctaagaaa
     1981 ggctgaatca tgagcagata ccaacagaaa tttatcgtac aggaattgga aaatcacgaa
     2041 ttcatctatc ccgattcgtt tggcgatatt ggatttacgt cgaacatcaa atcagccggt
     2101 aaatatgaca gctatgaaga cgcattcagc tcggcattgg aagagatagg cggggaattt
     2161 gtaattttcg gattctacga aaaagaagat taagtttaag aggctcggca ggcggtctct
     2221 aaaaccttca caaagcccgc aaacacattt ttttaaaaat ttcgt
//
LOCUS       DAWTQS010000123         2214 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.123, whole genome shotgun sequence.
ACCESSION   DAWTQS010000123 DAWTQS010000000
VERSION     DAWTQS010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.123"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(103..413)
                     /locus_tag="WNA25_002103"
                     /pseudo
     CDS             complement(103..413)
                     /locus_tag="WNA25_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261045.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            300..812
                     /locus_tag="WNA25_002104"
     CDS             300..812
                     /locus_tag="WNA25_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFA9824755.1"
                     /translation="MKNFNGKLILIGLMGAGKTTLGRQMAQRLDYRFYDSDHEIAAAA
                     GVPIPTIFEMEGEQGFRSRETAILKKLIVLPHIVLSTGGGAVLKEENRALIRKSGTVV
                     YLHAPPETLLERTRCDNSRPLLQVADPLAKLRELYAARDPVYRQTADFTVESANCRET
                     VQTLLKRLSR"
     gene            892..1971
                     /gene="aroB"
                     /locus_tag="WNA25_002105"
     CDS             892..1971
                     /gene="aroB"
                     /locus_tag="WNA25_002105"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFA9824756.1"
                     /translation="MKTLTVHTPSHSYPIFIGNGLLPQAGSLLKPHLGKRAAIITNET
                     VAPLYLGTLQTALDAAGVSHFSIILPDGEAHKNWQTLNLIFDGLMQNRAERKTTLIAL
                     GGGVIGDMVGFAAATYQRGAPFIQIPTTLLSQVDSSVGGKTAINHPLGKNMIGAFYQP
                     QAVLADLDTLHTLPARELSAGMAEVIKYGALGDIGFFEWLEQHMPELMALERAPLTQA
                     VYRCCQMKADIVAQDETEQGIRAWLNLGHTFGHAVEAEMGYGVWLHGEAVAAGCVLAA
                     RLSEQLGKTSAADTARLAALLEAAGLPSAPPVFAFEKWLAHMSHDKKVSGGIMRFIGL
                     NRLGEAVITEITDTDILRRTLQPYL"
BASE COUNT          543 a          692 c          557 g          422 t
ORIGIN      
        1 tattgataaa catattatct tcaatatatt caattggata attgtttacc taagcaaaga
       61 taattgcctt ttcctgacaa attaagtgaa atcaacggat tgtcaaaaca cagcctgaaa
      121 taaaaaacct ccctgatttc ttttattttt ccttaaaatc agaaaagttc gggatggtcg
      181 ggttattttt ccaaacgtac cgccgccctg ccgatttcgt ataaaatccc gccgtaaccc
      241 gacaagcccg aaccctgccg ccccgaaagg cggggggggt caaacattaa ggaaccgtga
      301 tgaaaaactt taacggcaaa ctcatcctca tcggactgat gggcgcgggc aaaactacgc
      361 tgggccggca aatggcgcag cggctggatt accgttttta cgacagcgat cacgaaatcg
      421 ccgcagcggc gggcgttccc atccccacca tatttgaaat ggaaggcgaa cagggattcc
      481 gttcgcgcga aaccgccata ctcaaaaaac tgattgtcct gccgcatatc gtcctgtcca
      541 ccggcggcgg cgcggtgtta aaagaagaaa accgcgccct tatccgcaaa agcggcacgg
      601 tcgtttatct gcacgccccg cccgaaaccc tgctcgaacg cacgcgctgc gacaacagcc
      661 gccctttgct gcaagttgcc gatcctttgg cgaaattgcg cgaactctac gccgcacgcg
      721 accccgttta ccgccaaacc gccgacttta ccgtagaatc ggcaaactgc cgggaaaccg
      781 tgcaaaccct gctcaaacgc ttatcccgat aaaccggcat atgcgccgct ccccgaaaac
      841 caaaccgcac cccgcccggc gggccggcgg ttcaaacttt aaggaacaac aatgaaaaca
      901 ctgaccgtac acacgccttc gcacagctac cccatcttta tcggcaacgg gctgctgccg
      961 caggcgggaa gcctgctcaa accgcatttg ggcaaacgcg ccgccatcat caccaacgaa
     1021 accgtcgccc cgctctacct cggcacgctt cagacggcat tggatgcggc aggcgtatcc
     1081 catttcagca tcatcctgcc cgacggcgag gcgcacaaaa actggcagac gctcaacctc
     1141 atctttgacg ggctgatgca aaaccgcgcc gaacgtaaaa ccacgctgat tgcgctgggc
     1201 ggcggcgtga tcggcgacat ggtcggcttt gccgccgcca cctaccagcg cggcgcgccg
     1261 ttcatccaaa taccgaccac gctgttgagt caggtcgact catcggtggg cggaaaaacc
     1321 gccatcaacc acccgctcgg caaaaatatg atcggcgcgt tttaccagcc gcaggcggtg
     1381 cttgcagatt tggacacgct gcacaccctg cccgcccgcg aactgtccgc cggtatggcg
     1441 gaagtcatca aatacggcgc gctcggcgac atcggctttt ttgaatggct ggaacagcat
     1501 atgcccgaac tgatggcact cgaacgcgcg cccctcaccc aagccgtgta ccgctgctgc
     1561 caaatgaagg cagacatcgt cgcccaagac gaaaccgaac agggcatacg cgcatggctc
     1621 aacctcggac acaccttcgg acacgccgtc gaagcggaaa tgggttacgg cgtatggctg
     1681 cacggcgagg ccgttgccgc cggctgcgtg ttggcggcgc gtttgtccga acaactgggc
     1741 aaaacctccg ccgcagatac cgcgcggctc gccgccctgc tcgaagccgc cggactgccg
     1801 tccgcaccgc ccgtgtttgc ctttgaaaaa tggctggcac atatgagcca cgacaaaaaa
     1861 gtcagcggcg gcatcatgcg ctttatcggt ctgaaccggc tgggcgaagc cgtcatcacc
     1921 gaaattaccg acacggacat cctccggcgc accctgcaac cgtatctctg atttcctctg
     1981 ccgatgtgtt gccgcgcggg tttgacgcac gatgatgact ttccatcatc attctccgcg
     2041 aaagcgggca tccggttatt ccatattgca gggatttatc cggaataacg acaacctgtc
     2101 cgccgtcatt tccgcgcaga tggaaatttg gaaattcaaa atcacaggaa tttatccgga
     2161 attatagcgg attaacaaaa atcaggacaa ggcggcgaag ccgcaggcag tacg
//
LOCUS       DAWTQS010000124         2069 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.124, whole genome shotgun sequence.
ACCESSION   DAWTQS010000124 DAWTQS010000000
VERSION     DAWTQS010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.124"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            511..1290
                     /locus_tag="WNA25_002106"
     CDS             511..1290
                     /locus_tag="WNA25_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HFA9824757.1"
                     /translation="MAASPEAKFTEEKILWVKHHTPKLITFAISRPESYRFKAGQFSR
                     LGFYEGKGFIWRAYSVVSAEYADTLEYFAVLIQDGPMSALFAKMQQGDTILLDKNATG
                     FLLPERFPDGKDLVMLCTGSGIAPFLSILEQPEIRQRFDTVNLIHSVSFPEELIFNDR
                     LAALSEHPLVGEYGHSFRFVPVTTRAANPSGLSGKRIPELLKNNSIEQALHTKLTPES
                     TRFMICGNPEMVKDTFQTLLDMGYAMHRNRIPGQIMMENGF"
     gene            1499..>2069
                     /locus_tag="WNA25_002107"
     CDS             1499..>2069
                     /locus_tag="WNA25_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HFA9824758.1"
                     /translation="MAAHNKLTQKQIEAAKADGKQSKLADGGGLYLLLHPNGSKYWRM
                     RYRHGGCEKTLALGVYPAVSLKQARELARAARAQTAAGIDPVAEHHRTRPGSGRSLPE
                     IARAWYGSRQGQRADNTLEGDRRSLAYLTDYYKDNTDINDITTAGVSKFIEHLNRINI
                     PACARRTVQILAQIWDYAVQRGIITDERRN"
BASE COUNT          642 a          597 c          406 g          424 t
ORIGIN      
        1 agcaaaataa gttttgatta taataaaaaa gaattcttaa ttagtgatat aacgaacttt
       61 aactgggatc atgtaaaact gtatataatc aattcagatt tccaaaaaat tgttttttat
      121 gataaaggta agatagtttt tgaggaatta atagaactag ataggaaagg gaaagtttta
      181 cctcaatatc tatttgattc atatttgaaa aatgtttaat attacgaatg tgattacaag
      241 aatggcaaga tgcaactttt aaaaaagaaa aattacattc ttttgataga tatttttatt
      301 attataaacc tataaactgt aggccgaaac ttttgtaaaa ctgtccatac tcattaccgc
      361 ctagtcggta atgcattttt tagtagtatt tgaaaaagta tgatgataaa aacggttaag
      421 cctaactttt tcataaaatc cagcagagta aggcagaaaa aaacacccaa attggctata
      481 atcccgacaa acacactcaa ggacaacaac atggcagcct cgcccgaagc aaaattcacc
      541 gaagaaaaga ttttgtgggt caaacaccac acgccgaaac tcatcacttt cgccatcagc
      601 cgtcccgaat cctaccgttt taaagccgga cagttctccc gactcggttt ctacgaaggt
      661 aaaggcttta tttggcgtgc ctattccgtc gtttccgcag aatatgccga cacgctcgaa
      721 tattttgccg tactcatcca agacggcccc atgtcggccc ttttcgccaa aatgcaacag
      781 ggcgacacca tcctgctcga taaaaatgcc actggctttc tcctgcccga acgcttcccc
      841 gacggcaagg atttggtgat gctctgtacc ggctcgggca tcgccccctt cctttccatt
      901 ctcgaacaac ccgaaatccg gcagcgtttc gataccgtca acctgataca ttccgtatct
      961 tttcccgaag aattgatttt caacgaccgg ctggccgcat tgtccgaaca tcccctggta
     1021 ggcgaatacg gacactcgtt ccgtttcgtc cctgttacca cccgtgccgc caacccctcg
     1081 ggcttaagcg gaaaacgcat tccggaactc ttaaaaaaca acagcatcga acaggcgctg
     1141 cataccaagc ttaccccgga atccacacgg tttatgattt gcggtaaccc ggaaatggtc
     1201 aaagacactt tccaaacgct gctcgatatg ggttacgcca tgcaccgcaa ccgcattccc
     1261 ggtcaaatca tgatggaaaa cggcttctaa aaacgaacgc tcccaacccc tacccacgcc
     1321 cacacacaaa acaaaataaa aaacagcgat atacaaaaac caatttgcaa aaacagacct
     1381 cacgcctata ctgccactca cagcaagcaa cacaaaaaag cgggtacaaa ttagggtaca
     1441 aaacacaagc ccgaacacag aagcacccca accaaaccca aaggcaaagg caaacaaaat
     1501 ggcggcacac aacaaactga cccaaaaaca aatagaagca gccaaggcag acggcaaaca
     1561 aagcaagctg gccgacggcg gcggcttata tctgctgctg caccccaacg gcagcaaata
     1621 ctggcggatg cgctaccggc acggcggatg cgaaaaaacc ctagccttgg gcgtatatcc
     1681 cgccgtcagc ctcaagcagg cccgcgaact tgcccgcgcc gcccgggcgc agaccgccgc
     1741 cggcatcgac cccgtcgccg aacaccaccg cacccgtccc ggcagcgggc gcagccttcc
     1801 cgaaatcgcc cgcgcgtggt acggcagccg tcaagggcaa cgcgccgaca acacactgga
     1861 aggcgaccgc cgttcactcg cctacctgac cgactactac aaagacaaca ccgacatcaa
     1921 cgacatcacc accgccggcg tatcaaaatt tatcgaacac ctcaaccgca tcaacatccc
     1981 cgcctgcgcc cggcgcaccg tccaaatcct cgcccaaata tgggactatg ccgtccagcg
     2041 cggcatcatt accgacgaac ggcgcaacc
//
LOCUS       DAWTQS010000125         1998 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.125, whole genome shotgun sequence.
ACCESSION   DAWTQS010000125 DAWTQS010000000
VERSION     DAWTQS010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.125"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            35..>1998
                     /locus_tag="WNA25_002108"
     CDS             35..>1998
                     /locus_tag="WNA25_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HFA9824759.1"
                     /translation="MNGQNQPEVKSSVPYSIKDKDRKRKYTHQNTQGGGGSVSFNNSD
                     ELVSQQSGTAVFGTATYLPPYGKVSGFDTDSLKGRANAVDWIRTTRIALAGYSYAGVI
                     CRNITGCPKLVYKTQFTFDQQGLKKKAGSKLDIYEDKSRENSPIYKLSDYPWLGVSFN
                     LGSENTVKDGKSFNKLISSFGEDNNNQTIVSTTEDYPISLGDGRREHTAVAYYLNAKL
                     HLLDKKQIQNITGKTVRLGVLKPSIDVKTQNTGLSGILNFWSKWDIKDNGQIPVKLGL
                     PEVKAGRCINEPNPNKNTQAPSPALTAPALWFGPGQNGKVQMYSASVSTYPGSSSSRI
                     FLQELKTQTEPGKPGRYSLKSLNDGEIKSRQPSFNGRQTIIRLDDGVHLIKLNGSKDE
                     VAAFVNLNGNNTGKNDTFGIVKEANVNLDADEWKKVLLPWTVRGPDNDNKFKSINQKP
                     EKYSQRYRIRDNNGNRDLGDIVNSPIVAVGGYLATAANDGMVHIFKKNGGSDERSYNL
                     KLSYIPGTMPRKDIQSQESTLAKELRAFAEKGYVGDRYGVDGGFVLRQVNLNGQDRVF
                     MFGAMGLGGRGAYALDLSKINGNYPAAAPLFDVKDGDNNGKNRVEVKLGYTVGTPQIG
                     KTQNGTYSAFLASGYAAKKIDDSTNKTALYV"
BASE COUNT          586 a          513 c          488 g          411 t
ORIGIN      
        1 ggcgatggcg caaacccgtg aatacgctat tatcatgaac gggcaaaacc agcccgaggt
       61 aaagtcgagt gtgccatatt caataaagga caaagacagg aagcgcaaat atactcatca
      121 gaacacacaa ggaggaggag gctctgtctc attcaacaat agcgatgagc ttgtttctca
      181 acaaagcggt actgccgttt ttggcacagc cacctacctg ccgccctacg gcaaggtttc
      241 cggttttgat accgatagtc tgaaagggcg cgccaatgcc gttgattgga ttcgtaccac
      301 ccgcatcgcg ctggcaggct acagctacgc cggtgtcata tgcagaaaca tcacaggctg
      361 tcccaaactt gtctataaaa cccaatttac cttcgatcaa caagggttga aaaaaaaggc
      421 aggcagcaag ctggatatat acgaagacaa aagccgcgaa aattcgccca tttacaaatt
      481 gtcggattat ccatggttgg gcgtgtcttt caatttgggc agcgagaata ccgtcaaaga
      541 tggcaaatca ttcaacaaat tgatatcttc ttttggtgaa gacaataata atcaaaccat
      601 tgtctctacg acagaagact accctatttc ccttggcgac gggcggcgcg aacataccgc
      661 cgtggcctat tatctgaacg ccaagctgca cctgctggat aaaaaacaga ttcaaaatat
      721 caccggcaaa acagtgcggt tgggtgtctt gaagccgagc atcgatgtga agacacaaaa
      781 tacggggctt agcggcattc taaatttttg gtctaagtgg gacattaaag ataacgggca
      841 gattccggtc aagctcggcc tgccggaagt caaagcaggc cgctgcatca acgaaccgaa
      901 ccccaataag aatacccaag ccccttcgcc ggcactgacc gcccccgcgc tgtggttcgg
      961 ccctgggcaa aatggtaagg tgcagatgta ttccgcttcg gtttctacct accccggcag
     1021 ttcgagcagc cgcatcttcc tccaagagct gaaaactcaa accgaacccg gcaaacccgg
     1081 ccgctattcc ctcaaatctt tgaatgatgg tgagattaaa agtcgacagc cgagtttcaa
     1141 cgggcggcaa acaatcatcc gattggatga cggcgtacat ttgatcaaac tgaatggaag
     1201 caaggatgag gtcgccgctt ttgtcaattt aaatggaaac aacaccggca aaaacgacac
     1261 tttcggcatt gttaaggaag cgaacgtcaa tcttgacgcc gacgagtgga aaaaagtgct
     1321 gctgccttgg acggttcggg gtcccgataa tgacaataaa tttaaatcaa ttaaccaaaa
     1381 accagaaaaa tacagccaaa gataccgcat ccgcgacaac aacggcaatc gcgatttggg
     1441 cgacatcgtc aacagcccga ttgtcgcggt cggcgggtat ttggcaaccg ccgcgaacga
     1501 cgggatggtg catatcttca aaaaaaacgg cggcagtgat gaacgcagct acaatctgaa
     1561 gctcagctac atccccggca cgatgccgcg caaggatatt caaagccaag aatccaccct
     1621 tgccaaagag ctgcgcgcct ttgccgaaaa aggctatgtg ggcgaccgct acggcgtgga
     1681 cggcggcttt gtcttgcgtc aagttaactt aaacggacaa gaccgcgtgt ttatgttcgg
     1741 cgcaatgggc cttggcggca gaggcgcgta tgccttggat ttaagcaaaa tcaacggaaa
     1801 ttatccggcc gccgctcccc tgtttgatgt caaagatggc gataataacg gcaaaaatcg
     1861 cgtggaagtg aaattaggct acaccgtcgg cacgccgcaa atcggcaaaa cccaaaacgg
     1921 cacatactcc gccttcctcg cctccggtta tgcggctaaa aaaattgacg actcaacaaa
     1981 taaaaccgcg ctgtatgt
//
LOCUS       DAWTQS010000126         1772 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.126, whole genome shotgun sequence.
ACCESSION   DAWTQS010000126 DAWTQS010000000
VERSION     DAWTQS010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.126"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(136..1104)
                     /gene="ispH"
                     /locus_tag="WNA25_002109"
                     /gene_synonym="lytB"
     CDS             complement(136..1104)
                     /gene="ispH"
                     /locus_tag="WNA25_002109"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFA9824760.1"
                     /translation="MNGKTIILANPRGFCAGVDRAISIVERALEEFGAPVYVRHEVVH
                     NKFVVDNLREKGAVFIEDLAEVPPGATLVYSAHGVSKAVQQEAAERGFRVFDATCPLV
                     TKVHKEVARLDAQNCEIIMIGHKGHAEVEGTMGQLAPGKMLLVETVGDVAKLEVRNPD
                     KLAYVSQTTLSVDETKDIIAALNARFPNIRNPHKEDICYATTNRQTAVKELAEQCDIV
                     IVVGSPNSSNSNRLREVAASRGIDAYMVDNASYLQRTWFEGKSKVGVTAGASAPEVLV
                     REVLAAIRGWGHETVREGGGAEESIVFVLPKELRREGETKPDLCKR"
     gene            complement(1134..1628)
                     /locus_tag="WNA25_002110"
     CDS             complement(1134..1628)
                     /locus_tag="WNA25_002110"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HFA9824761.1"
                     /translation="MSSSVSSKTRYWVLALAAIVLDQWSKWAVLSSFQYRERVNVIPS
                     FFDLTLVYNPGAAFSFLADQGGWQKYFFLVLAVAVSAYLVRAILRDEFAALGKIGAAM
                     IIGGASGNVIDRLIHGHVVDFLLFYWQNWFYPAFNIADSFICVGAVLAVLDNIVHRKD
                     SKKT"
BASE COUNT          393 a          521 c          447 g          411 t
ORIGIN      
        1 gcggtttgcc ggaagaaggt gctcatggga aatcccctaa atgccttggt gggaatttag
       61 gggattttgg ggatttttgc aaaggtctcg gcatttgtat tgcccaaaca tccgatgcct
      121 gcgttaacgc ctgcgtcaac gtttgcacaa atcgggtttg gtttcgccct cgcggcgcag
      181 ctccttgggc aggacgaaca cgatgctttc ttccgcgccc cccccttcgc gcacggtttc
      241 atgcccccat ccgcgtatgg ctgccaatac ttcccgcacc aacacttcgg gcgcggacgc
      301 gcctgccgtt acgccgactt tgcttttgcc ttcaaaccac gtgcgttgca ggtaggacgc
      361 gttgtccacc atatacgcat cgattccgcg cgatgccgcc acttcgcgca ggcggttgct
      421 gttggacgaa ttgggcgaac cgaccacaat cacgatgtcg cactgttccg ccagctcttt
      481 gacggcggtt tgccggttgg tcgtcgcata gcagatgtct tccttgtgcg gattgcggat
      541 attggggaaa cgcgcgttca gcgcggcgat gatgtctttg gtttcatcga ccgagagcgt
      601 ggtttggctg acataggcga gtttgtcggg gtttctgact tcgagttttg ccacatctcc
      661 gaccgtttcg accaaaagca ttttgcccgg tgcaagctgc cccatcgtgc cttcgacctc
      721 ggcgtgcccc ttatgcccga tcatgatgat ttcacagttt tgggcatcca gtcgggcgac
      781 ttccttatgc actttcgtta ccagcgggca agtcgcatca aatacccgga aaccgcgctc
      841 cgccgcttcc tgctgcaccg ccttcgatac gccgtgtgcc gaataaacca gtgtcgcgcc
      901 cggcggcact tccgccaagt cttcgataaa caccgcgcct ttttcgcgca ggttgtccac
      961 gacgaatttg ttgtggacga cttcgtggcg cacataaacc ggcgcgccga attcttccaa
     1021 agcacgttcg acaatactga ttgcccgatc cacaccggcg cagaagccgc gcggattggc
     1081 aaggatgatg gtttttccgt tcataagttt tgcattccgt gttcagacgg cattcacgtt
     1141 tttttgctat ctttgcgatg gacgatattg tcaagcaccg ccaacaccgc accgacgcag
     1201 ataaagctgt cggcaatatt aaaggcggga taaaaccaat tttgccaata aaacaataag
     1261 aaatcgacga catgaccgtg tatcaggcga tcgatgacat tgcccgacgc accgccgata
     1321 atcattgccg ccccgatttt gccgagggct gcaaactcgt cgcgcaagat ggcgcgtacc
     1381 aaatacgcgc tcaccgccac cgccagcacc aaaaaaaagt atttctgcca gccgccctga
     1441 tcggcaagga agctgaatgc cgcgcccggg ttgtacacca gcgtcagatc gaaaaacgaa
     1501 gggatgacgt tgacgcgttc ccgatactga aacgacgaca gcaccgccca cttcgaccac
     1561 tggtccagca cgatggcggc aagtgccaat acccaatagc gcgttttact tgaaacagat
     1621 gaagacatat ttttcaacag ccggataaaa gagtgccatt ctacccgaaa accccctttc
     1681 ctgtacccga aacggcaaat gccgtaatct tgaaacccgt cattcccaca aaagtgggaa
     1741 tccaggacgt aaaatctcaa gaaacctttt tt
//
LOCUS       DAWTQS010000127         1578 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.127, whole genome shotgun sequence.
ACCESSION   DAWTQS010000127 DAWTQS010000000
VERSION     DAWTQS010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.127"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            <1..1114
                     /gene="mafB"
                     /locus_tag="WNA25_002111"
     CDS             <1..1114
                     /gene="mafB"
                     /locus_tag="WNA25_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HFA9824762.1"
                     /translation="YPPPGGARDIYSYYVKGTSTKTKINTVPQAPFSDRWLKENAGAA
                     SGFLSRADEAGKLIWENDPDKNWRANRMDDIRGIVQGAVNPFLTGFQGLGVGAITDSA
                     VNPVTDTAAQQTLQGINDLGNLSPEAQLAAASLLQDSAFAVKDGINSARQWADAHPNI
                     TATAQTALAVAEAAGTVWRGKKVELNPAKWDWVKNTGYETTVTRKTLDGQIAGKNKPI
                     KPSSGDYVLPVGRQIIDPTRTSFSQATVSYQKRGANYNYDSLVAAMNEKKSWVGDRVD
                     VVNMPDGAPTSMDNTRIMAAREAGVKVEANVHNFNDRLSSKERIRFKHDGIEPQTWGE
                     AIQLRIRKQETQKGVPEGWSKRFPNGSIYDVKVLRK"
     gene            1111..1482
                     /locus_tag="WNA25_002112"
     CDS             1111..1482
                     /locus_tag="WNA25_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HFA9824763.1"
                     /translation="MIKQNSFVPYPEAMLPKGFKYPQSYLKLAQSTHAINYDEQYSFP
                     WWFENAESNISEVIDIYFEITGIPNLLPFARNQEWAACFDISDKSGNPKIIVVNLDNT
                     KYYETFENFDTWLKEAENDGW"
BASE COUNT          516 a          303 c          354 g          405 t
ORIGIN      
        1 ttatccgccc cccggaggag caagggatat atacagctac tatgtcaaag gaacttctac
       61 aaaaacaaag ataaacactg ttccgcaagc ccctttttca gaccgctggc taaaagaaaa
      121 tgccggtgcc gcttccggtt ttctcagccg tgcggatgaa gcaggaaaac tgatatggga
      181 aaacgacccc gataaaaatt ggcgggctaa ccgtatggat gatattcgcg gcatcgtcca
      241 aggtgcggtt aatccttttt taacgggttt tcagggattg ggagttgggg caattacaga
      301 cagtgcggta aacccggtca cagatacagc cgctcagcag actctacaag gtattaatga
      361 tttaggaaat ttaagtccgg aagcacaact tgctgccgcg agcctattac aggacagtgc
      421 ttttgcggta aaagacggca tcaattccgc cagacaatgg gctgatgccc atccgaatat
      481 aacagcaaca gcccaaactg cccttgccgt agcagaggcc gcaggtacgg tttggcgcgg
      541 taaaaaagta gaacttaacc cggccaaatg ggattgggtt aaaaataccg gctatgaaac
      601 aactgttacc cgaaagactt tagatgggca aatagctggc aagaacaagc ctattaaacc
      661 ttcttcagga gattatgtct tgccggtagg cagacaaatc atagatccta ctcgtacatc
      721 tttttcacaa gctacagtta gctatcagaa gcgtggtgca aattacaact atgattctct
      781 tgtagctgca atgaacgaaa aaaaatcatg ggttggcgac agggttgatg ttgttaatat
      841 gcctgatgga gcacctacta gtatggataa cacgcgtatt atggcagcac gtgaagcagg
      901 ggtaaaagtg gaagcgaatg ttcataattt taatgaccga ttatcatcaa aagagagaat
      961 caggtttaag catgatggta ttgagcctca aacttgggga gaagctatcc agctacgaat
     1021 tagaaagcaa gaaacacaaa aaggagttcc agaagggtgg agcaaaagat ttcctaacgg
     1081 aagtatttat gatgtaaagg tacttaggaa atgataaaac aaaatagttt tgttccgtat
     1141 cctgaagcaa tgcttcctaa aggatttaaa tatccgcaaa gttatttaaa attagctcaa
     1201 tccactcatg ccattaacta cgatgaacaa tattcttttc cttggtggtt tgaaaatgca
     1261 gaaagcaata tatcagaagt aattgacatt tattttgaaa taactggcat tccaaaccta
     1321 ttaccttttg ctagaaacca agagtgggct gcctgttttg atatttcaga taaatcaggt
     1381 aatcctaaaa ttatagtagt taatttagat aatacaaaat attacgagac ttttgaaaat
     1441 tttgatactt ggctaaaaga agctgaaaat gatggttggt agcagccgta ggtcggattc
     1501 tcgaatccga cattttccaa cagcggcatt tcggaaacga tagacgcgtc aaatattttt
     1561 gtcggataca agtatcca
//
LOCUS       DAWTQS010000128         1539 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.128, whole genome shotgun sequence.
ACCESSION   DAWTQS010000128 DAWTQS010000000
VERSION     DAWTQS010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.128"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(61..320)
                     /locus_tag="WNA25_002113"
                     /pseudo
     CDS             complement(61..320)
                     /locus_tag="WNA25_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711407.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            508..852
                     /locus_tag="WNA25_002114"
     CDS             508..852
                     /locus_tag="WNA25_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HFA9824764.1"
                     /translation="MNLPFRAMVSDLGGTLLTPEHLVGDLTIDTLRVLEQKGVDIILA
                     TGRNHTDMSSILGKIGAERAVMITSNGARVRDLQGNLLYSNSLPEELVLELYKTSYVG
                     NLIQIPRFCRHQ"
     gene            806..1339
                     /locus_tag="WNA25_002115"
     CDS             806..1339
                     /locus_tag="WNA25_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01621 family pseudouridine synthase"
                     /protein_id="HFA9824765.1"
                     /translation="MWEILFRYQDFVAINKPQGISVHRSGGEVSLTATLAAQLGVEKV
                     WLLHRLDKQAGGILLFALNPQSAAVLAAQFAERKMKKTYLALSDRKPSKKQGWIKGGM
                     EKSRRGMWKLTRNMENIAVTRFFSIRISEKMRLFILEPHTGKTHQLRVVMKSLGSSIF
                     GDSLYGGTESETMFLYA"
BASE COUNT          467 a          319 c          320 g          433 t
ORIGIN      
        1 cccggcagct aaaaatcctg cgggatcggt gtggaattta gggataatct agtacagccc
       61 ctatttattt tgtgccttac tttcagtacc aattgccacc gcacttaaat ttgttgcctg
      121 agaagatgtc cccactgcaa ttcctttcgt accactggca gcactgcttt tccctaagga
      181 aataccaaaa ttcgatgcat tagcagaagt acccaatgcc actcaatttt agatgccgat
      241 gctttatcgt tccatgataa ggagccatta ttaccttctt gcaaagtaga atccgcccca
      301 taagaagaca aagcactcat taacaaaata ccagccgcta tcgcggacga cctatataca
      361 taacattact tcatatcatt tcattcagta tcaatagtat aggataactc ccccccccgt
      421 caatcgaaat aattcccgta ttttaatgtc gtattgttcc tgtatattta gtgatgtgat
      481 aagtatagtt tattttttga ggtttttatg aatttacctt ttcgggcaat ggtgtctgat
      541 ttaggtggca cacttttaac acctgaacat ttagtgggtg atcttaccat tgatactctg
      601 agagtattag agcaaaaagg cgtagatatt attttggcaa caggacgcaa tcataccgat
      661 atgtcatcca ttcttggaaa aatcggtgcg gaacgtgcgg ttatgattac atcaaacggt
      721 gctcgtgtac gggatttaca gggtaatttg ctttatagca atagtttgcc tgaagaatta
      781 gttctcgagc tttacaaaac atcatatgtg ggaaatctta ttcagatacc aagattttgt
      841 cgccatcaat aaacctcaag gcatatccgt ccaccgttcc ggcggcgaag tcagtctgac
      901 ggcaacactt gcggcacagt tgggcgtaga aaaggtatgg ttgctgcaca ggttggacaa
      961 gcaggccgga ggcatattgc tgtttgccct taatccccaa agtgccgctg ttcttgccgc
     1021 acagtttgcc gaaaggaaaa tgaagaaaac ctatttggct ttgtccgacc gcaaaccgtc
     1081 caaaaaacaa ggatggataa aaggcggcat ggaaaaatcc aggcgcggga tgtggaagct
     1141 gacgcgcaat atggaaaata ttgccgttac ccggtttttc agtatccgca tcagtgaaaa
     1201 aatgcggctg ttcatccttg agccacatac gggtaagaca catcagttga gggtggtaat
     1261 gaagagtctg ggcagctcta tatttgggga cagcctgtat ggaggaacag aatccgaaac
     1321 tatgttcctg tatgcgtgaa aaatacaatt tacctatcaa caacaagaaa tcgaaattac
     1381 cgcacctttg aaaaatgcat ggcaggccga aaatatctct catgcactgg aagaatttta
     1441 tatggcaaaa gcaggttaat agatataaat caattaaaac aatatgataa atatttttat
     1501 aaaatttttt caaaaaacgg ttgaccgcaa atctcttta
//
LOCUS       DAWTQS010000129         1483 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.129, whole genome shotgun sequence.
ACCESSION   DAWTQS010000129 DAWTQS010000000
VERSION     DAWTQS010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.129"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(33..392)
                     /locus_tag="WNA25_002116"
     CDS             complement(33..392)
                     /locus_tag="WNA25_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824766.1"
                     /translation="MIHKPRYIKIVDENGDFTRVLRLHKFPDTSKVFYFEPMFWLKDG
                     RLARKDSLFEVDYIYGADGCGFLPSNLTEFRKYCRKKHQKFKDDEVLVNRYAVDFLGA
                     KEPPYDDRHVTSVKYFV"
     gene            complement(513..>1483)
                     /locus_tag="WNA25_002117"
     CDS             complement(513..>1483)
                     /locus_tag="WNA25_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824767.1"
                     /translation="DRPLYFCHIDGLDKRKFNARELAEGQIMSAPLRDVIPEGAVLIV
                     GEAHYTYPVRAAGRPVPPYIQELTELRHHGHTVILMTRHPSQLDIFVRNLVSKHVHLE
                     RKAIGMKQYYWYKCVTSLDNPAGVSGVEAANWKPPKEAFKYYKSSSRHQKFKKKVPWA
                     VWALIAVVGFVGWKSYGMFQVYSKATDSRIEQEAQKESVVQTMTEQTASSETAPFEHS
                     DNLKPEDFVPTLPEKPESKPIYNTVRQVKTFEQIAGCIDGGKSDCTCYSNQGTPLKEI
                     TKIMCKEYVKNGLPFNPYKDERQRTEQAAQSAKADKPQVLVMGGKS"
BASE COUNT          336 a          357 c          334 g          456 t
ORIGIN      
        1 gggtagtggc tgaatttgtg attttggttt tatcaaacaa aatatttgac tgaagtcaca
       61 tggcggtcgt catatggggg ttccttcgca cccaaaaaat cgaccgcgta acggtttact
      121 aaaacttcat cgtccttaaa cttttggtgc tttttccggc aatattttct gaactccgtt
      181 aaatttgacg gcaagaaccc gcaaccgtct gcaccgtaaa tgtaatcaac ttcaaacaaa
      241 ctgtctttcc gggcaagccg accatcttta agccagaaca taggctcgaa ataaaaaact
      301 ttcgacgtgt ccgggaactt atggagacgg agaacacgtg tgaaatcccc gttttcatct
      361 acaattttga tatatcttgg tttgtgaatc atgacatcct cagatttagt attcagaata
      421 tgattttaaa aagaactttc tgctttacga ctccgccgcc gattccttca aacggttttc
      481 cgcgctcttc agttgtcgta cattaaattt tattaggact ttccgcccat tacgagaact
      541 tggggcttgt ccgctttcgc ggactgtgcc gcctgttccg tcctttgccg ttcgtccttg
      601 taaggattga aaggcaaccc gtttttcaca tattctttac acattatctt tgttatttct
      661 ttcaagggtg ttccttgatt tgaatagcat gtgcaatctg attttccgcc gtctatgcat
      721 ccggcgattt gctcaaaggt ttttacttgt cggactgtgt tataaatagg cttgctttcg
      781 ggcttttcgg gcaaagtcgg cacaaagtct tcaggtttca gattgtcgga atgctcaaaa
      841 ggcgctgttt ctgatgatgc cgtctgctcc gtcatcgtct gcacaacgct ttctttttgc
      901 gcttcctgct caatccggct gtctgtggct ttgctgtaaa cttgaaacat gccgtaactt
      961 ttccagccta caaaccctac aaccgcaatc aacgcccaaa ccgcccaagg cacttttttc
     1021 ttgaactttt ggtgccggct tgatgattta tagtatttga aggcttcttt aggcggtttc
     1081 caatttgcgg cttctacgcc gcttacgccc gcgggattgt ccaacgaggt tacgcattta
     1141 taccaataat actgtttcat gccgattgcc ttgcgttcaa ggtgtacatg ctttgaaaca
     1201 aggttgcgga cgaatatatc aagttggctc gggtgccgcg tcatcaaaat gacggtatgc
     1261 ccgtgatggc ggagttctgt cagttcctga atatagggcg gaacgggacg gcctgccgcg
     1321 cgtaccgggt aagtgtagtg cgcttcgcca acaatcagca ccgcgccttc cggtatgaca
     1381 tcacgaagcg gggcggacat gatttgccct tccgccagtt cgcgggcatt gaattttcgt
     1441 ttgtccaatc cgtcgatatg gcagaaataa agcggccggt ctg
//
LOCUS       DAWTQS010000130         1379 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.130, whole genome shotgun sequence.
ACCESSION   DAWTQS010000130 DAWTQS010000000
VERSION     DAWTQS010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.130"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(36..959)
                     /locus_tag="WNA25_002118"
     CDS             complement(36..959)
                     /locus_tag="WNA25_002118"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFA9824768.1"
                     /translation="MLQRTLAKSIGVTGVGLHSGERVALTLHPAPENSGISFRRTDLD
                     GEMGEQIKLNPYLINDTRLSSTIVTDKGLRVGTIEHIMSALSAYGIDNALIELNAPEI
                     PIMDGSSLPFIYLLQDAGVVDQKAQKRFLKILKPVEIKEAGKWVRFTPYDGFKVTLTI
                     EFDHPVFNRSPPTFEIDFAGKSYIGEIARARTFGFMHEVEMMRAHNLGLGGNLNNAIV
                     IGDTDVLNPEGLRYPDEFVRHKILDAIGDLYIVGHPIVGAFEGYKSGHAVNNALLRAV
                     LADETAYEWVEFADSDDLPDAFHELNIRNCG"
     gene            1091..1192
                     /locus_tag="WNA25_002119"
                     /pseudo
     CDS             1091..1192
                     /locus_tag="WNA25_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT          334 a          338 c          376 g          331 t
ORIGIN      
        1 atctgtttga cggcatttgt ttggaaaagg gggcgttatc cgcaatttct gatgttcagc
       61 tcgtgaaatg cgtcgggcaa atcatcgctg tcggcaaatt ccacccattc gtaagccgtt
      121 tcgtctgcca aaactgcgcg caaaagggcg ttgttgacgg cgtgtcccga tttgtagcct
      181 tcaaacgcgc cgacaatcgg atgtccgacg atatacaaat cgccgatggc gtcgaggatt
      241 ttgtggcgca caaactcatc gggatagcgc aagccttcag gattcaggac atccgtgtcg
      301 ccaatcacga tggcgttgtt caaattgccg cctaagccga gattgtgggc gcgcatcatt
      361 tccacttcgt gcataaagcc gaaagtgcgc gcgcgcgcga tttcgccgat gtaggatttg
      421 ccggcgaaat caatttcaaa cgtaggcggg ctgcggttga aaacaggatg gtcgaattcg
      481 atggtcagcg ttaccttaaa accgtcatac ggcgtaaatc ggacccattt gcccgcttct
      541 ttgatttcga cgggtttgag gattttcaaa aaacgctttt gcgccttttg gtctacaacg
      601 cccgcatctt gcaggaggta aataaacggc aggctggagc cgtccataat cgggatttcg
      661 ggcgcgttca actcaatcag cgcattgtcg atgccgtagg cggacagtgc ggacatgatg
      721 tgttcgatgg tgccgacgcg caggcctttg tcggtaacga tggtggatga aaggcgggta
      781 tcgttgatca aataagggtt cagcttgatt tgttcgccca tctcgccgtc caaatcggtg
      841 cggcggaagg aaatcccgct gttttcaggc gcggggtgca gggtcagcgc gacgcgttcg
      901 cccgaatgca gcccgactcc ggtaacgccg atggatttcg ccaaagttct ttgcagcata
      961 aaccgcttcc ttatcaatgg ggtaagtttc ggaataatac gataaaaccg gaaaaacagg
     1021 ctatgttttt caatagtatt tgccaatgta tccgtttcca acacgtaagc cgcatgaaaa
     1081 tgtatggcgg attaacgaaa atcaggacaa ggcggcgaag ccgcagacag tacggatagt
     1141 acgaaaaggc gaggcaacgc cgtaccggtt taaatttaat tcactataca tcccgtcatt
     1201 cccacgaaag tgggaatcca ggactcagga ccggagaaac ctttttaccc gataagtttc
     1261 cgtgccgaca gacctagatt cccgctttcg cgggaatgac gaagttttcc ggccatatgc
     1321 cgtgattcgg gcaatacccg gcaacggctg atttttgacc ttcggccaaa aggatggct
//
LOCUS       DAWTQS010000131         1345 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.131, whole genome shotgun sequence.
ACCESSION   DAWTQS010000131 DAWTQS010000000
VERSION     DAWTQS010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.131"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(12..209)
                     /locus_tag="WNA25_002120"
     CDS             complement(12..209)
                     /locus_tag="WNA25_002120"
                     /inference="COORDINATES: protein motif:HMM:NF025063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HFA9824769.1"
                     /translation="MDNAAMESFFGTLKSECFHTCKYDSVTESEAALHEYIRYYNNDR
                     IKLKLKGLSPVQYRIQSLKAA"
     gene            complement(254..832)
                     /locus_tag="WNA25_002121"
     CDS             complement(254..832)
                     /locus_tag="WNA25_002121"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFA9824770.1"
                     /translation="MPSVELLLGIVGLPRSTFYYQLAVQSAEGKYADLKRHIHDIYQR
                     YKGRYGYRRIAAAIRHAGIPVNHKKVSRLMAKTGLKAVIRRRKYRSFKGEVGKVAPNI
                     LQRCFHSEKPNEKWVTDVTEFDVGGEKIYLSPIMDLFNGEIVSYRIQTRPTFDLAGEI
                     LKGAPEKPGPSEKPMLHSDQGWQYQMFFIKSS"
     gene            complement(837..1265)
                     /locus_tag="WNA25_002122"
     CDS             complement(837..1265)
                     /locus_tag="WNA25_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFA9824771.1"
                     /translation="MSDSLVRKWVARYRLHGESGIKRRKHTTKYSVEYKLEAIRPVTG
                     QGMSQKAAADQLNLPDCSVLPQWLRLYRLNGINGLKPKPKGRKPVKKQYPPQTKKADY
                     LKTKEELFAELAYLKAEAAVLKKLDALKEVRQKERNSSQG"
BASE COUNT          299 a          342 c          310 g          394 t
ORIGIN      
        1 ggacagttta atcaagcggc tttcagggac tgaattctgt actgaacagg gctcagtcct
       61 tttaatttca acttgattct atcgttgttg tagtaacgga tatattcgtg cagtgccgct
      121 tccgattcgg taacggaatc atatttgcac gtatggaaac attccgattt caacgttccg
      181 aagaaacttt ccattgccgc attgtccaag cagtttccct tgcgggacag actctgaacc
      241 agaccgttgc ctttcaactg cttttgataa aaaacatctg atattgccaa ccttgatccg
      301 aatgcagcat cggcttttca gacggccccg gtttctccgg cgcacctttc agtatctcgc
      361 cggccaaatc gaaagtcggg cgggtttgaa tacggtaact gacgatttcc ccgttaaaca
      421 aatccataat cggagaaagg tatatctttt ctccgcctac atcgaactcg gtaacgtccg
      481 ttacccattt ctcattcggc ttttctgaat ggaaacagcg ttgcaggatg ttcggcgcaa
      541 ctttgccgac ttctcctttg aacgagcggt atttgcgccg ccgtatcact gccttcagcc
      601 ccgtcttcgc catcagacgg ctgactttct tgtgattgac cggtattcct gcgtgacgga
      661 tggctgccgc aatcctccgg tagccgtatc ttcccttata tcgttgataa atatcatgga
      721 tatgtcgttt caaatcggca tatttgcctt ctgccgattg gacggccaat tggtaataga
      781 aggtgctgcg tggcagtccg acaatcccca acagcagttc aacggatggc atttccttaa
      841 ccctgcgacg agttgcgttc tttctgccgc acttctttca aggcatcgag cttttttagg
      901 acagccgctt ccgctttaag gtaagccaat tccgcaaaca gttcttcctt ggttttcaga
      961 tagtcggctt ttttcgtttg cggcggatac tgttttttca cgggctttct tcctttgggt
     1021 ttgggcttta aaccgttaat accattcaaa cggtagaggc gcaaccattg cggcaaaacg
     1081 gagcagtcgg gcagattcag ttggtctgcg gcagcttttt gggacattcc ctgccccgtc
     1141 accgggcgga ttgcctcaag tttgtattcg accgaatatt ttgtcgtatg ctttctgcgt
     1201 ttgatgccgc tttcaccgtg taatctgtat cttgccaccc atttgcgtac caatgaatcg
     1261 gaaatggaaa aatatagtga attaacaaaa atcaggacaa ggcggcgggc cgcaggcagt
     1321 acaaatggta cggaaccgat ccgcc
//
LOCUS       DAWTQS010000132         1213 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.132, whole genome shotgun sequence.
ACCESSION   DAWTQS010000132 DAWTQS010000000
VERSION     DAWTQS010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.132"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(246..656)
                     /locus_tag="WNA25_002123"
     CDS             complement(246..656)
                     /locus_tag="WNA25_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824772.1"
                     /translation="MKIYNLDNIITSSALSLYKSTWVKLDLISCAKFIIEGTNFTFDW
                     DEGIENWIVFFHKNKVIGYLCAKLPFAILDNEQLKNQLYHKYPHVLTLQIKNIETEKI
                     SLNVNICRLTLCPSFHDEHDTLVCSVQDFYVNTV"
     gene            complement(660..>1213)
                     /locus_tag="WNA25_002124"
     CDS             complement(660..>1213)
                     /locus_tag="WNA25_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HFA9824773.1"
                     /translation="WRGKKVELNPTKWDWVKNTGYKKPAARHMQTLDGEMAGGNKPAK
                     QATPNGKAGVSTQQSLQNSLVPQKNYTHTTYNGGSAITDGKYITDPLSMVRHKPDASS
                     LDKIDSKSLFKSDVDAEKATLDAARYADKHNLWDSQNKAKVPTNDVVGYTDGKPTHII
                     NIYRRKPNKDGIRPVHGTPGKER"
BASE COUNT          357 a          224 c          187 g          445 t
ORIGIN      
        1 tgctgaacaa tgttttgtag aacttgctca aaatccatgt ttcgccattt tcctttatat
       61 atacctaatg cagcacgggt tacgatttcg tttaaatcat aaatagccat ttacgtcctc
      121 tgctaatttt cagcgagtag attgggttac caaacccaac aaaaaagcag cctgaaaaat
      181 ttcaggatgc ttgttttatg ttttttatca atgatttagg ttttaatcca gcctgcgctt
      241 gctagttaaa cagtatttac ataaaaatct tgcactgaac aaacaagcgt atcatgttca
      301 tcatgaaatg atgggcataa cgttaatcta caaatgttta catttaaaga tattttttca
      361 gtttcaatat ttttaatttg taaggttaat acatgaggat atttgtgata tagctgattt
      421 tttaattgtt cgttgtctaa tatagcaaaa ggtaattttg cacacaaata accaataact
      481 ttgtttttat gaaaaaaaac tatccaattt tcaattcctt catcccaatc aaaagtaaaa
      541 ttcgttcctt ctatgataaa tttagcacaa gatattaaat ctaacttaac ccaagtggat
      601 ttatacagac tcaatgctga tgaagttata atattatcta aattataaat tttcataaat
      661 caacgttcct tacctggtgt tccatgcaca ggtctaatcc catctttatt cggctttcta
      721 cgataaatgt taattatatg tgtcggtttt ccgtctgtat agccaactac gtcgttcgtt
      781 ggaacttttg ccttattttg actatcccat aaattatgtt tatctgcata tctagcagca
      841 tctaatgtag ctttttccgc atcaacatct gatttaaata aacttttgct atctatcttg
      901 tctagactag atgcatcagg tttgtgtcgt accatactga gagggtcagt aatatatttg
      961 ccatctgtaa tagctgatcc accattatag gtagtatgcg tataattttt ttgtggaacg
     1021 agactatttt gcaaagattg ttgtgtagaa acgccagctt tgccattagg agtagcttgc
     1081 ttcgctggct tattcccacc tgccatctcc ccatctaaag tctgcatatg gcgggcagca
     1141 ggttttttat agccggtatt tttaacccaa tcccatttag tcgggttaag ttctactttt
     1201 ttacctctcc aaa
//
LOCUS       DAWTQS010000133         1169 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.133, whole genome shotgun sequence.
ACCESSION   DAWTQS010000133 DAWTQS010000000
VERSION     DAWTQS010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.133"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(160..426)
                     /locus_tag="WNA25_002125"
                     /pseudo
     CDS             complement(160..426)
                     /locus_tag="WNA25_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(463..953)
                     /locus_tag="WNA25_002126"
                     /pseudo
     CDS             complement(463..953)
                     /locus_tag="WNA25_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          287 a          315 c          269 g          298 t
ORIGIN      
        1 cactatatct tgagaccttt gcaaaattcc cttccctccc gacagctgaa accctgacac
       61 agattttcgg ctgtttccgc caccaatcac tcctaactct acccaaatac ccccttaatc
      121 ccccttgaat acccgataat caggcatccg ggccgccttt taggcggcag cgggcgcact
      181 tagcctgttg gccgctttca acaggttcaa acacatcgcc ttcaggtggc tttgcgcact
      241 cactttaatc agtccgaaat aggccgcccg ggcatagcgg aatttacggt gcagcgtacc
      301 gaagctttgt tcgaccacat aacgggtctt cgacaaatat cggttgcgtt tggtttgcgc
      361 ctccggcagc gggcggttgc ggcgggcttt gcgcataatg ccgtccagca gccgatgctc
      421 tttcagtttt ccgtaggtcg gattctcgaa tccgacatta cttcaatcgt attcaataga
      481 aaagtccgca ttgccgccac cccaattatg cggataaata ccctgtttga cataacggtg
      541 aaacgtagaa aaccgccaat cggaaatttg tcctacatag ccatgtttga ccggattgaa
      601 atgcagataa tcaaaatggc aggcaaaatc ggcctcaccg cggatagtat gttcccaaaa
      661 gcgtttttcc aaagcctgag attgccgccg attaaatatt ggctgtgccg cttgatttgc
      721 cgccagcgtt ccgaataagc agaatcattg tccggcagcc gccatatggt atgcagatga
      781 tcgggcatca acacccatgc caaaatttca aacggattcc gttcgcgcac cgccattacc
      841 gcctgccgta aagccaaacg caccgcatca tcggtcaaaa tcttctgccg tttattggtt
      901 acaaccgtaa aaaagtaagt gccgccattg cggtaaaaac gacggtattt catagtatta
      961 tgctcggaat gattttgtag gccggattct tgaattcgac attttggaca ttgctgcaat
     1021 ggattgcaat gatgggaatg ttaaaggttt tgtcggatac aagtatccga cctacgcttg
     1081 ctgaaccgtc attcccacgg aagtgggaat ctaggacgcg gggtttcagt catttccgat
     1141 agattcccgc cgcgtcgggg gtccggatt
//
LOCUS       DAWTQS010000134         1150 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.134, whole genome shotgun sequence.
ACCESSION   DAWTQS010000134 DAWTQS010000000
VERSION     DAWTQS010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.134"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(719..1015)
                     /locus_tag="WNA25_002127"
     CDS             complement(719..1015)
                     /locus_tag="WNA25_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824774.1"
                     /translation="MQADLAYAYEHITRDYPEATGANQGKISTVSDYFKNIRTRSVHP
                     RLALGYDFGGWRVFSAPAVAAGLHQSLQYYRSRHCRRFRQIHQFRRITPIPQKP"
BASE COUNT          242 a          283 c          369 g          256 t
ORIGIN      
        1 cggttgtaaa tccaatctgt ttttcaaacc gcctgcaacc gccaaatctg ccgcaacctg
       61 ccccctcccc cgtgggggag agggctcccc gaatttcgac aatctttccc aaatccctta
      121 gtcttctaaa atacaagcct tgcagctttc tagctctctc ccacagggag agaggactat
      181 gatgcccgcc ggcggcatcg caccttatcc gcgcgctccg gaacagggct gtatggcaga
      241 tgcgtaggat gggcgtaggt gggttgtggc gggttgctat taggaatacc tgaatcgtca
      301 ttcccacgaa agtgggaatc tagaacgcag ggttggggca accgttttat ccgataagtt
      361 cctatgccga cagacctgaa ttcccgcctg tgcgggaatg acggcgcata agtttccgcg
      421 cggacaaatc cggattcctg tctgtgtggg aatgacgaat ccgtccgcac ggaaacctgc
      481 accgcgtcat tcccccgaaa gtgggaatct agaaacttaa cgctacggca atttttggaa
      541 atgactgaaa ccgaacggac tggattcccg cctgcgcggg aatggcgggt tttaggatta
      601 cggtgtatcg ggacggttcg ggtattcttg acaggatggg ttctcaagat gttgcgtagt
      661 gtcgggctcg caagggggtg catagagagg gttgcagggc gggcttcagc ccgccgcatc
      721 aaggtttttg gggaataggc gtgattcggc ggaattgatg gatttgacgg aatcggcggc
      781 aatggcggga tcggtaatat tgaagggatt ggtggaggcc ggcggcaacg gcgggggcgg
      841 aaaagacgcg ccagccgccg aaatcgtagc cgagggcgag tcgggggtgg acggagcggg
      901 tgcggatgtt tttgaaataa tcgcttaccg tgcttatttt gccttggttt gcaccggttg
      961 cttcgggata atcgcgggtg atgtgctcgt aggcgtaagc cagatccgcc tgcacatacg
     1021 ggccgcgccc atggtcttca cccgccgcct gccaagcatc agggcgagca ggaggggttt
     1081 gtattgcatg gttcggcttt cggaaaaaat cggataatgc tgaaggccgc gcgaaagcgg
     1141 ccggatgttc
//
LOCUS       DAWTQS010000135         1144 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.135, whole genome shotgun sequence.
ACCESSION   DAWTQS010000135 DAWTQS010000000
VERSION     DAWTQS010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.135"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            32..>1144
                     /gene="tuf"
                     /locus_tag="WNA25_002128"
     CDS             32..>1144
                     /gene="tuf"
                     /locus_tag="WNA25_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFA9824775.1"
                     /translation="MGHVDHGKTTLTAALTTILAKKFGGAAKAYDQIDNAPEEKARGI
                     TINTSHVEYETETRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREH
                     ILLARQVGVPYIIVFMNKCDMVDDAELLELVEMEIRDLLSSYDFPGDDCPIVQGSALK
                     ALEGDAAYEEKIFELATALDSYIPTPERAVDKPFLLPIEDVFSISGRGTVVTGRVERG
                     IIHVGDEIEIVGLKETQKTTCTGVEMFRKLLDEGQAGDNVGVLLRGTKREDVERGQVL
                     AKPGTITPHTKFKAEVYVLSKEEGGRHTPFFANYRPQFYFRTTDVTGAVTLEKGVEMV
                     MPGENVTITVELIAPIAMEEGLRFAIREGGRTVGAGV"
BASE COUNT          300 a          322 c          293 g          229 t
ORIGIN      
        1 acgtagcaaa ccgcacgtaa acgttggcac catcggtcac gttgaccatg gtaaaaccac
       61 cctgactgct gctttgacta ctattttagc taaaaaattc ggcggcgctg caaaagctta
      121 cgaccaaatc gacaacgcac ccgaagaaaa agcacgcggt attaccatta acacctcgca
      181 cgtagaatac gaaaccgaaa cccgccacta cgcacacgta gactgtccgg gtcacgccga
      241 ctacgttaaa aacatgatta ccggcgccgc acaaatggac ggtgcaatcc tggtatgttc
      301 tgctgccgac ggccctatgc cgcaaacccg cgaacacatc ctgctggccc gtcaagtagg
      361 cgtaccttac atcatcgtgt tcatgaacaa atgcgacatg gtcgacgatg ccgagctgtt
      421 ggaactggtt gaaatggaaa tccgcgacct gctgtccagc tacgacttcc ccggcgacga
      481 ctgcccgatc gtacaaggtt ccgcactgaa agccttggaa ggcgatgccg cttacgaaga
      541 aaaaatcttc gaactggcta ccgcattgga cagctacatc ccgactcccg agcgtgccgt
      601 ggacaaacca ttcctgctgc ctatcgaaga cgtgttctcc atttccggcc gcggtaccgt
      661 agtcaccggc cgtgtagagc gaggtatcat ccacgttggt gacgagattg aaatcgtcgg
      721 tctgaaagaa acccaaaaaa ccacctgtac cggcgttgaa atgttccgca aactgctgga
      781 cgaaggtcag gcgggcgaca acgtaggcgt attgctgcgc ggtaccaaac gtgaagacgt
      841 agaacgcggt caggtattgg ccaaaccggg tactatcact cctcacacca agttcaaagc
      901 agaagtgtac gtattgagca aagaagaggg cggccgccat accccgtttt tcgccaacta
      961 ccgtccccaa ttctacttcc gtaccactga cgtaaccggc gcggttactt tggaaaaagg
     1021 tgtggaaatg gtaatgccgg gtgagaacgt aaccattact gtagaactga ttgcgcctat
     1081 cgctatggaa gaaggtctgc gctttgcgat tcgcgaaggc ggccgtaccg tgggtgccgg
     1141 cgtg
//
LOCUS       DAWTQS010000136         1082 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.136, whole genome shotgun sequence.
ACCESSION   DAWTQS010000136 DAWTQS010000000
VERSION     DAWTQS010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.136"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(26..310)
                     /locus_tag="WNA25_002129"
     CDS             complement(26..310)
                     /locus_tag="WNA25_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824776.1"
                     /translation="MNRAALFNRYPEWIIGQDGASRFITHCRYPRLIAKIHRQTDGEC
                     PGGHYRHSENGITLYDFIFFGGKPADEARFAAVLTETCRRAVKKIGSVPD"
     gene            complement(347..610)
                     /locus_tag="WNA25_002130"
     CDS             complement(347..610)
                     /locus_tag="WNA25_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824777.1"
                     /translation="MADILPSDKRRQPPKTKGNPINIIEQETKRTELRKIDAEIAKII
                     ADAHKINAESVKIAQESRWYPMMAATGLVTAIAAVLALIFKFA"
     gene            complement(630..878)
                     /locus_tag="WNA25_002131"
     CDS             complement(630..878)
                     /locus_tag="WNA25_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824778.1"
                     /translation="MHGFRDLFTNISLTAGKDTLTTDLALGHISRAALQRAGFSSLHH
                     YLTADSYRLQERRELAEWYGRRHRQAYEAAAQNTCNKT"
     gene            complement(839..>1082)
                     /locus_tag="WNA25_002132"
     CDS             complement(839..>1082)
                     /locus_tag="WNA25_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFA9824779.1"
                     /translation="FTVPLSDWAVEILRELHTQTGDNIHLFPGIRPRRKPAPHPDHIS
                     IKFAHNAIRRLGYDGSTPANPNTPCTDSATSSPTSA"
BASE COUNT          172 a          226 c          375 g          309 t
ORIGIN      
        1 tcatatttcg agggtaacat atctgctaat ctggtacaga ccctattttt ttgactgccc
       61 ggcggcaggt ttcggtaagg acggcggcaa atcgggcttc gtctgccggt ttgccgccga
      121 aaaaaataaa atcgtaaagg gtgatgccgt tttcggaatg gcggtaatgc ccgcccgggc
      181 attcgccgtc tgtctgcctg tggattttgg caatcaggcg gggatagcgg caatgggtaa
      241 tgaaacggct tgcgccgtct tgcccgataa tccattcggg gtagcggttg aatagtgcgg
      301 ctctgttcat tttgttgagg gataaagccc ctcgcggggc ttgtggtcag gcaaatttga
      361 atatcagtgc caacacggcg gcgatggcgg taaccagacc ggtggccgcc atcatgggat
      421 accagcggga ctcttgggct atttttacgg attcggcgtt gattttgtgc gcgtcggcaa
      481 tgattttggc gatttcggca tctatttttc ttaactcggt gcgttttgtt tcttgttcga
      541 ttatgtttat tggatttcct ttcgtttttg gcggttgccg ccgcttgtcg gatggcagga
      601 tgtctgccat gtatgtatgt taataccttt taggttttat tgcaagtgtt ttgggcggcg
      661 gcttcgtatg cttggcggtg gcggcggccg taccattcgg cgagttcgcg ccgctcttgg
      721 aggcggtagc tgtcggcggt caggtagtgg tgcaggcttg agaagccggc gcgttggagg
      781 gcggcgcggc tgatgtggcc aagggcgagg tctgttgtga gggtgtcttt tccggcggtc
      841 aggctgatgt tggtgaagag gtcgcggaat ccgtgcatgg tgtgtttgga tttgccgggg
      901 tgctgccgtc atagcccagt cgtcggatgg cgttgtgggc gaatttgatg ctgatgtggt
      961 cgggatgggg ggcgggtttg cgccgtgggc ggatgcctgg gaaaaggtgg atgttgtcgc
     1021 cggtctgtgt gtgcagctct cggagtattt ctactgccca gtccgacagt gggacggtaa
     1081 ag
//
LOCUS       DAWTQS010000137          975 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.137, whole genome shotgun sequence.
ACCESSION   DAWTQS010000137 DAWTQS010000000
VERSION     DAWTQS010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..975
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.137"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            141..368
                     /locus_tag="WNA25_002133"
     CDS             141..368
                     /locus_tag="WNA25_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFA9824780.1"
                     /translation="MNQKQTQCKQIVDYIRNKGCITSLEAYQNLKVTQLAARITDLEG
                     RGFVFAKPRMKAGGRGKPVTHYSIVKNGAEV"
     gene            365..>975
                     /locus_tag="WNA25_002134"
     CDS             365..>975
                     /locus_tag="WNA25_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFA9824781.1"
                     /translation="MSARLMGMAFKTGIPRGQRFVLVKLCDCANDEGLCYPSQETLAE
                     DTGFAETAVRQHIKWLKDNNFIKSARRQRGRERKSDIYRINVALLEKCYAEAAKRKAA
                     RQAKMWEEPLDYEPSDYEPSDFDAKNHQILSGEPSDFALRTVRFCAKNHQILSGEPSD
                     FDGSLYVEPSVEPSVEPSGSNARGARAPAGPHPAKPQTAPPETA"
BASE COUNT          259 a          241 c          300 g          175 t
ORIGIN      
        1 cggttgcaac ggggtgacgg caaatcggaa agacggctga ccgcccggac agacgggcgg
       61 ccgataaaga aaaacccgca cggggcgggt aatccccctg aattgcaggg aagcggttca
      121 ggtaacggcg aaaggcgatt atgaatcaaa aacaaacgca atgcaaacaa attgtcgatt
      181 acatccgtaa caagggatgc atcacatccc ttgaggctta tcagaacctg aaggtgacgc
      241 agcttgcggc acggataacc gacttggaag gcaggggctt cgtgtttgcc aagccgcgca
      301 tgaaggcggg cggccgcggg aagcctgtta cgcattattc gattgtcaaa aacggagcgg
      361 aagtatgagt gcgaggctga tggggatggc tttcaaaacg ggtatcccga gggggcagcg
      421 ttttgttttg gtgaagttgt gcgactgcgc caacgacgag ggcttgtgtt atccgtcgca
      481 agaaacgctg gcggaagata cgggcttcgc cgaaaccgcc gtacggcagc atatcaagtg
      541 gctgaaggat aacaatttca tcaagtccgc ccggcggcag agggggcggg agaggaagtc
      601 cgacatctac cgcatcaacg tcgccctgct tgaaaaatgc tatgcggagg cggcaaaacg
      661 gaaggcggcg cggcaggcaa aaatgtggga agaaccattg gattacgaac cgtcggatta
      721 cgaaccgtcg gattttgacg ctaagaacca tcagattttg agcggcgaac cgtcagattt
      781 tgcgctaaga accgtcagat tttgcgctaa gaaccatcag attttgagcg gtgaaccatc
      841 agattttgac ggttccttat atgtagaacc gtcagtagaa ccgtcagtag aaccgtcagg
      901 atcaaatgcg cgcggcgcgc gcgcccctgc cggaccgcac cctgcgaaac cgcaaacggc
      961 gcctcccgaa accgc
//
LOCUS       DAWTQS010000138          850 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.138, whole genome shotgun sequence.
ACCESSION   DAWTQS010000138 DAWTQS010000000
VERSION     DAWTQS010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..850
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.138"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            152..712
                     /locus_tag="WNA25_002135"
     CDS             152..712
                     /locus_tag="WNA25_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenoid-binding protein"
                     /protein_id="HFA9824782.1"
                     /translation="MKKIIFAALAAAAVGTASATYKVDEYHANVRFAIDHFNTSTNVG
                     GFYGLTGSVEFDQAKRDGKIDITIPVANLQSGSQPFTGHLKSADIFDAAQYPDIRFVS
                     TKFNFNGKKLVSVDGNLTMRGKTAPVKLKAEKFNCYQSPMAETEVCGGDFSTTIDRTK
                     WGVDYLVNAGMTKNVRIDIQIEAAKQ"
BASE COUNT          229 a          288 c          180 g          153 t
ORIGIN      
        1 cacggataac accgcgcttt ttcatccgcc ctgttccgcc cgcccgttcg tcaaccgcct
       61 gcaaacccac cgtttaccgg caggaaatgc cataaatccc cgcctatgtt aggataaacg
      121 cctttaccgg cccacacaaa ggattatcat catgaaaaaa atcatcttcg ccgcgctcgc
      181 agcggcagcc gtcggcactg cctccgccac ctacaaagtg gacgaatatc acgccaacgt
      241 ccgtttcgcc atcgaccact tcaacaccag caccaacgtc ggcggttttt acggtctgac
      301 cggttccgtc gagttcgatc aagcaaaacg cgacggcaaa atcgacatca ccattcccgt
      361 cgccaacctg caaagcggtt cgcaaccctt caccggccac ctgaaatccg ccgacatctt
      421 cgatgccgct caatatccgg acatccgctt cgtttccacc aaattcaact tcaacggcaa
      481 aaaacttgtt tccgttgacg gcaacctgac catgcgcggc aaaaccgccc ccgtcaaact
      541 caaagccgaa aaattcaact gctaccaaag cccgatggcg gaaaccgaag tttgcggcgg
      601 cgacttcagc accaccatcg accgcaccaa atggggcgtg gactacctcg ttaacgccgg
      661 tatgaccaaa aacgtccgca tcgacatcca aatcgaagct gcaaaacaat aagccttgat
      721 gcgaatcagc acaaatgccg tcttcatcaa accgccctgc caaccgtaag ccgatatggc
      781 ggagcacaac ccgccgaaag atattttggc ggcaggcggg gcttgccgga aagtccggaa
      841 agtaacgacc
//
LOCUS       DAWTQS010000139          776 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.139, whole genome shotgun sequence.
ACCESSION   DAWTQS010000139 DAWTQS010000000
VERSION     DAWTQS010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.139"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            <1..375
                     /locus_tag="WNA25_002136"
     CDS             <1..375
                     /locus_tag="WNA25_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HFA9824783.1"
                     /translation="YFVKGSQAETEGVWVHNDCPYGKGNQRYKDAPYHGKNDNSVKSR
                     APTNGQAVLDNSVQVKSTSSQRVGVDKTNNEIVVLNQTRIFNDGSAEYHGHVRNWKNL
                     HTDQQNALKKAGLVNSKGKIKK"
     gene            372..707
                     /locus_tag="WNA25_002137"
     CDS             372..707
                     /locus_tag="WNA25_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824784.1"
                     /translation="MMADKEQVEKMIYSENKDCVIDGMLGMTFSSEEDEIPWISEKLT
                     ELSKHKDLDIARLSLTCFGHLARMHENIGDCDKVIALLRSKQGNPDFQGFAEDALDDI
                     SFFIFKKRP"
BASE COUNT          260 a          125 c          178 g          213 t
ORIGIN      
        1 tacttcgtca agggcagtca ggcggaaacg gaaggggttt gggttcataa tgattgtccg
       61 tatggaaaag gcaaccaacg atataaagat gctccttatc atggtaaaaa tgataattct
      121 gtgaaaagta gagcgccaac aaatgggcaa gctgtccttg ataactctgt tcaagttaaa
      181 tcgacatctt ctcaaagagt tggagttgat aaaaccaata atgaaatcgt tgtattaaac
      241 caaactagaa tttttaatga cggttctgct gagtatcatg ggcatgtaag gaactggaaa
      301 aatttacata ctgatcaaca aaatgcttta aagaaagcag gattggttaa ttcaaaagga
      361 aaaatcaaaa aatgatggct gataaggaac aagtagaaaa gatgatttat tctgaaaata
      421 aagattgtgt tattgatggt atgctcggta tgaccttcag ctcggaggag gatgaaatcc
      481 cttggatttc cgagaaattg acggagttgt cgaagcataa agacttggat attgccagat
      541 tgtcattaac ctgtttcgga catctcgcca ggatgcatga aaatatcggc gattgcgata
      601 aagtgattgc tttattgcgc tcaaaacaag gtaatccgga ttttcaaggt tttgccgaag
      661 atgcgttaga cgatatttcc ttctttatat ttaaaaagcg tccgtaagcg cggattaaac
      721 ccggaaatcc gatgcgtctg aaagtacatt ccggctttca gacggcatcg gtaaca
//
LOCUS       DAWTQS010000140          770 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.140, whole genome shotgun sequence.
ACCESSION   DAWTQS010000140 DAWTQS010000000
VERSION     DAWTQS010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.140"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            <1..>770
                     /locus_tag="WNA25_002138"
     CDS             <1..>770
                     /locus_tag="WNA25_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HFA9824785.1"
                     /translation="GTPIAKIEAPGGKGGLSSPTLVDKDLDGTVDIAYAGDRGGNMYR
                     FDLSNSDPNKWSAKAIFEGTKPITSAPAVSRLKDKRVVIFGTGSDLTEDDVLDTKEQY
                     IYGIFDDDKGTGTVKVTVQNGTAGGLLEQNLMQENKTLFLNKRSDGSGSKGWAVKLTG
                     GQRVTVKPTVVLRTAFVTIRKYNDGGCGAETAILGINTADGGALTPRSARPIVPEANT
                     AVAQYSGHKTTSKGKSIPIGCMEKGGKTVCPNGYVYDKP"
BASE COUNT          211 a          200 c          212 g          147 t
ORIGIN      
        1 ggtacgccga ttgcaaaaat cgaagcaccc ggcggcaaag gcgggctttc gtcccccacg
       61 ctggtggata aagatttgga cggcacggtc gatatcgcct atgccggcga ccggggcggc
      121 aatatgtacc gctttgattt gagcaattcc gatcctaata aatggtctgc aaaggctatt
      181 ttcgaaggca caaaaccgat tacctccgcg cccgccgttt cccgactgaa agacaaacgc
      241 gtggttatct tcggtacggg cagcgatttg accgaagatg atgtactcga tacgaaagaa
      301 caatatattt acggtatctt tgacgacgat aaggggacgg ggacggttaa ggtaacggta
      361 caaaacggca cggcaggcgg gctgctcgag caaaacctta tgcaggaaaa taaaacatta
      421 ttcctgaaca agagatccga cggttcgggc agcaagggct gggcggtgaa attgacaggc
      481 ggacaacgcg ttaccgtcaa accgaccgtg gtattgcgta ccgccttcgt aaccatccgc
      541 aaatataacg acggcggctg cggcgcggaa accgccattt tgggcatcaa taccgccgac
      601 ggcggcgcat tgactccgag aagcgcgcgc ccgattgtgc cggaagccaa tacggctgtc
      661 gcacaatatt ccggccataa gacaacctcc aaaggcaaat ccatccctat aggttgtatg
      721 gaaaaaggcg gtaaaaccgt ctgcccgaac ggatatgttt acgacaaacc
//
LOCUS       DAWTQS010000141          765 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.141, whole genome shotgun sequence.
ACCESSION   DAWTQS010000141 DAWTQS010000000
VERSION     DAWTQS010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.141"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            565..765
                     /locus_tag="WNA25_002139"
                     /pseudo
     CDS             565..765
                     /locus_tag="WNA25_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
BASE COUNT          200 a          209 c          187 g          169 t
ORIGIN      
        1 ccggttgtgc cgatgggttg ccctgccgct ccctgagcta cgcaacggtt gtctgccttg
       61 ggcgggcgcg ggtggtggcc gggcggtttg tcgctatgat acggaagttc cgatatacaa
      121 gcggtgggct gaagcctacc atttccatca atccaacatt tctaccgttt tcattgaatc
      181 catcgaatcc gcccctttcg acaatccgac cctatacaac tgaaccgtca ttcccgcaca
      241 ggcgggaatc tagacccgtc ggcacggaaa cctgcatccc gtcattccca cgaaagtggg
      301 aatccggaac gcaaaatcta aagaaaccgt tttacccgat aagtttccgc actgacagac
      361 ctagattccc gcctgcgcgg gaatgacggt atatcggttt ccgttcggac aaatgtctgc
      421 gcaggaataa atggccgcgt ttgaattctc ggttaattcc atttattagc cgcttgcttc
      481 tgtttgcatg aaagccggga ttattgaata aaatacattt gcacggccgg ttaggtttgc
      541 ccgccggaaa tttaaacacc gccaaaaaaa tcagccgtta ccgagtattg cccgaatcac
      601 ggcgaatggc cggaagacaa cgacaaggcc ggcgtggcat cccccaccga catcaaaggc
      661 aaatatgtta aagaggttga agttaaaaac ggcgtcgtta ccgcccaaat gaaaccagac
      721 ggcgtaaaca aagaaatcca aggcaaaaaa ctctccctgt gggcc
//
LOCUS       DAWTQS010000142          735 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.142, whole genome shotgun sequence.
ACCESSION   DAWTQS010000142 DAWTQS010000000
VERSION     DAWTQS010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.142"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            248..589
                     /locus_tag="WNA25_002140"
     CDS             248..589
                     /locus_tag="WNA25_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824786.1"
                     /translation="MTKLYAEIAEMEAQDDGTVKVWGYASSEAVDSDGEVIAAAAMKA
                     AIPDYMKFGEVREMHGSNAAGTAIEINVEDDGRTFFGAHIVDPVAVEKVKTGVYKGFS
                     IGGSVTAVCLG"
BASE COUNT          203 a          181 c          193 g          158 t
ORIGIN      
        1 aaaataggga gggatgtcgt gatatttgaa aaatcaataa acggctggaa ataccgcgcc
       61 gtaatggaaa tcagaaccac gcaaaaggag cttgccttga acagccttta tacaacagac
      121 aggattgaat agtcatgaca aaacctgcga cgagtaactg tcaatctttc gattccgtcc
      181 tcatacatcc taagcgtctt tgcaaattct gttcagttat accatttata cagacaggag
      241 caaaccaatg acgaagttat acgcagaaat cgccgaaatg gaggcgcagg acgacggcac
      301 ggtcaaagtt tggggttatg cctcaagcga ggcggtcgat tcggacggcg aagtcatcgc
      361 ggcggcggct atgaaggcgg cgattcccga ttatatgaag ttcggcgagg tgcgcgagat
      421 gcacggcagc aacgcggcgg gaacggcgat tgaaatcaac gtggaagacg acggcagaac
      481 ctttttcggg gcgcatatcg tcgatcccgt tgcagtggag aaagtcaaaa cgggcgttta
      541 caagggcttt tccatcggcg gcagcgttac cgctgtctgc ttaggttgaa tcggcattcg
      601 tacgattacg gcacaccgcc taatctgtac ctgcgtctgt tgcgcccaat accaaaggct
      661 acctgaaaat ctcccaaact tttcaggtag ccttatcacg ttttcaactg ccgccacacg
      721 cccaaagacc gcctg
//
LOCUS       DAWTQS010000143          709 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.143, whole genome shotgun sequence.
ACCESSION   DAWTQS010000143 DAWTQS010000000
VERSION     DAWTQS010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.143"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
BASE COUNT          171 a          182 c          172 g          184 t
ORIGIN      
        1 ggtgcaggtt tccgtacgga tggattcgtc attcccgcgc aggcgggaat ccggacctta
       61 gaacaacagc aatattcaaa gattatctga aagtccggga ttctggattc ccgctttcgc
      121 gggaatgacg gttcggttgc tacggttact gtcaggtttc ggttatgttg gaatttcggg
      181 aaacttatga atcgtcattc ccatgcaggc gggaatctgg aatttcaatg cctcaagaat
      241 ttatcggaaa aaaccaaaac ccttccgccg tcattcccac tttcgtggga atgacgggat
      301 gtaggttcgt aggaatgacg tggtgcaggt ttccgtgcgg atggattcgt cattcccgcg
      361 caggcgggaa tccggtccgt tcggtttcag ttatttccga taaattcctg ctgcttttta
      421 tttctagatt cccacttccg tgggaatgac ggcggagggg ataagttctt gcaatctaaa
      481 atttcgtcat ttctataaaa tagcaaaccg aaacagaaac ttaaaaacag aaacctgaaa
      541 cagcaacctg aaaccccgtc attcccgcgc aggcggaaat ccggcgggcc gtagggtgtg
      601 cttcggcccg ccatttccat caatccaaca tgtctaccgt tttcatcgaa tccatcgaat
      661 ccgccttttc gaccacccgg ccctacgcaa ccgaaccgtc attcccgcg
//
LOCUS       DAWTQS010000144          706 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.144, whole genome shotgun sequence.
ACCESSION   DAWTQS010000144 DAWTQS010000000
VERSION     DAWTQS010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.144"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            152..367
                     /locus_tag="WNA25_002141"
                     /pseudo
     CDS             152..367
                     /locus_tag="WNA25_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038789.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          213 a          145 c          137 g          211 t
ORIGIN      
        1 ttcggcattg ccgacaatgg ccgttgcgtc aaaagtcaac ttggatatgt ccaatcctac
       61 ggtgttacgc atgggattac ccttatatat tgcgtcccta agaaggggcg attaacaaaa
      121 attaatgccc tttcttaggg acgcaatata taaggtttat accgtcgttg tccctaatgc
      181 gcaatcagcg acattgttgc caattatccg aaagaaagtt aagcctgatg gcattgtata
      241 cacggatacc tttcgtagtt atgatgtgct tgatgttagt gaatttagcc atttacgtaa
      301 gtttaacggc attcccaaag agcatttggg gctatattta aagaaatgcc aatggcattt
      361 gaaatagaat ctcaaattta gattttaaga caattagtta atgggaattt ggtctggtta
      421 tctagtacaa ccccaaaagt ttttatataa aaacagtttg gtaagttctc ttcgtgaagt
      481 gcttatgttt tgccacaaaa gttccataat catggcggca tcacccaagc tccatccttc
      541 caagaaaatg gcatctttgt tgtggttgaa aatcgactcc atacgatttt ggacaagcat
      601 agggatagat gttctgaaaa acggcagcgc gggcattctc caaccacctt cccaaatctt
      661 ataatacaaa cccgaagacc tttgcaaaat tcccctaaaa tcccct
//
LOCUS       DAWTQS010000145          655 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.145, whole genome shotgun sequence.
ACCESSION   DAWTQS010000145 DAWTQS010000000
VERSION     DAWTQS010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.145"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            <1..>655
                     /locus_tag="WNA25_002142"
     CDS             <1..>655
                     /locus_tag="WNA25_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HFA9824787.1"
                     /translation="LFSSLLFSSAAQAASEDGGRGPYVQADLAYAAEHITHDYPEPTG
                     ANQGKKISTVSDYFRNIRTHSIHPRVSVGYDFGGWRIAADYARYRKWNNNKYSVNTKK
                     VGEKNNGNTNVARYLKAENQENGTFHAASSLGLSAVYDFDTGSRFKPYAGVRVAYGHV
                     KHQVRSVESETTIVLSTPKGVQTPGRHIQGPTKKPAHHESRSISSLGFGAVAGVGIDI
                     "
BASE COUNT          183 a          187 c          164 g          121 t
ORIGIN      
        1 tctcttctct tctcttctct tctcttccgc agcgcaggcg gcaagtgaag acggcggccg
       61 cggcccgtat gtgcaggcgg atttagccta cgccgccgaa cacattaccc acgattatcc
      121 ggaaccaacc ggtgcaaacc aaggcaaaaa aataagcacg gtaagcgatt atttcagaaa
      181 catccgtacg cattccatcc accccagggt gtcggtcggc tacgacttcg gcggctggag
      241 gatagcggca gattatgccc gttacagaaa atggaacaac aataaatatt ccgtcaacac
      301 aaaaaaggtg ggagaaaaaa ataacggaaa cacaaacgtg gcgcgatatc tgaaggcgga
      361 aaatcaggaa aacggtacgt tccacgccgc ctcctcgctc ggcttgtccg ccgtttacga
      421 tttcgatacc ggttcccgct tcaaacccta tgcaggcgtg cgcgtcgcct acggacacgt
      481 taaacatcag gttcgttcag tggaaagcga aaccacgatt gtactcagta caccgaaagg
      541 cgttcaaacg ccaggcaggc acatacaagg tccgaccaaa aaacctgccc atcacgaaag
      601 ccgcagcatc agcagcttgg gcttcggggc agtggcaggc gtaggcatcg acatc
//
LOCUS       DAWTQS010000146          604 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.146, whole genome shotgun sequence.
ACCESSION   DAWTQS010000146 DAWTQS010000000
VERSION     DAWTQS010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.146"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            <1..403
                     /locus_tag="WNA25_002143"
     CDS             <1..403
                     /locus_tag="WNA25_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HFA9824788.1"
                     /translation="RGNDEAILLAEGQKSAVTEYYLNNGKWPADNGAAGVASSDKIKG
                     KYVQKVEVAKGVVTAQMASTGVNKEIQGKKLSLWAKRQAGSVKWFCGQPVTRNDAKDD
                     TVTAAAGNGGKNNAIDTKHLPSTCRDEHDAT"
BASE COUNT          165 a          171 c          153 g          115 t
ORIGIN      
        1 gcgcgggaat gacgaagcca tccttttggc cgaaggtcaa aaatcagccg ttaccgagta
       61 ttacctgaat aacggcaaat ggcccgccga caacggcgct gccggcgtgg catcctccga
      121 caaaatcaaa ggcaaatatg ttcagaaagt tgaagtcgca aaaggcgtcg ttaccgccca
      181 aatggcttca accggcgtaa acaaagaaat ccaaggcaaa aaactctccc tgtgggccaa
      241 gcgtcaagcc ggttcggtaa aatggttctg cggacagccg gttacgcgca acgacgccaa
      301 agacgacacc gtcaccgccg ccgccggcaa cggcggcaaa aacaacgcca tcgacaccaa
      361 gcacctgccg tcaacctgcc gcgatgaaca tgatgccacc taaggcaaat taggccttaa
      421 attttaaata aatcaagcgg taagtgattt tccacggccg cccggatcaa cccgggcggc
      481 ttgtctttta agggtttgca aggcgggcgg ggtcgtccgt tccggtggaa ataatatatc
      541 gattgcgctt caaggccctg catgtgcctc attgccaccc gtttaaacac ggtttttatc
      601 tgac
//
LOCUS       DAWTQS010000147          603 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.147, whole genome shotgun sequence.
ACCESSION   DAWTQS010000147 DAWTQS010000000
VERSION     DAWTQS010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.147"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            <1..429
                     /locus_tag="WNA25_002144"
     CDS             <1..429
                     /locus_tag="WNA25_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T cell/B cell stimulating protein TspB"
                     /protein_id="HFA9824789.1"
                     /translation="NPDTDGQPGTSPDSPAVPDRPNGRHRKERKQGEDGGLSCDYFPE
                     ILACQEMGKPSDRMFHDISIPQVTDDKTWSSHNFLPSNGVCPQPKTFHVFGRQYRASY
                     EPLCVFAEKIRFAVLLAFIIMSAFVVFGSLGGNKCHYLPA"
     gene            408..>603
                     /locus_tag="WNA25_002145"
     CDS             408..>603
                     /locus_tag="WNA25_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HFA9824790.1"
                     /translation="MPLLAGLIPLLGILLKMLIVRIILATGLTFVTYAGYLAALEKFK
                     GYTANAINSMPSDILNLLLIS"
BASE COUNT          150 a          156 c          141 g          156 t
ORIGIN      
        1 aatcccgata cggacggaca gcccggaaca agccccgatt ccccggccgt tccggaccgc
       61 ccaaacggca ggcatcgcaa agaaaggaaa caaggcgaag acggcgggct ttcgtgcgat
      121 tattttccgg aaatcctagc ctgtcaggag atgggcaaac cttcggaccg catgtttcac
      181 gatataagca taccgcaggt tacagacgat aaaacatggt cttcacataa ctttttaccg
      241 tctaacggcg tatgtccgca gccgaaaacc tttcatgttt tcggcaggca atatcgggca
      301 agctatgaac cgttgtgcgt gtttgccgaa aaaatccgtt ttgccgtact gctcgccttt
      361 atcattatgt cggcttttgt cgttttcggt tcgttggggg ggaataaatg ccattacttg
      421 ccggcctgat tccactttta ggcatacttc tgaaaatgct gattgtcaga atcatccttg
      481 caacaggtct gacattcgta acctatgccg ggtatctcgc cgcactggaa aagttcaaag
      541 gctacacggc aaatgcgatc aattccatgc cttccgacat attgaacctt cttttaattt
      601 cgg
//
LOCUS       DAWTQS010000148          589 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.148, whole genome shotgun sequence.
ACCESSION   DAWTQS010000148 DAWTQS010000000
VERSION     DAWTQS010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.148"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            complement(238..444)
                     /locus_tag="WNA25_002146"
     CDS             complement(238..444)
                     /locus_tag="WNA25_002146"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824791.1"
                     /translation="MLTACANSTPPLTTAVKPPADLVRPCPKLPHLEGNTGADVLPWS
                     LQVIGLYKDCRARHGALVRALGAD"
     gene            complement(395..>589)
                     /locus_tag="WNA25_002147"
     CDS             complement(395..>589)
                     /locus_tag="WNA25_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9824792.1"
                     /translation="AAVEHAEHARKSSAAYQAQKAAREEKERVRYVQTLKIIEKPVYR
                     NACFDADGVRELNAAVDDGG"
BASE COUNT          108 a          189 c          165 g          127 t
ORIGIN      
        1 gaagcctgcc cgcagggtgc ggcatcggtt gttgtccgga tcggtacggc attttcattt
       61 ccgcccatta ttggtgcgct taataatttt gtataacgcc cccttcgcgc aaacaaaacg
      121 gcaaaaattt gccgcgtccg cccgatatgt aaaaaatcgg ggggaatgcc gacgcttccc
      181 ccgatgcggc cgccaacccg gaaccgacgg ttcggttgca aacttccggt tggcaactca
      241 atccgcgccc aacgcccgta ccagcgcgcc gtgccgcgcc ctgcagtcct tgtacaagcc
      301 gatgacttgc aacgaccacg gcagcacgtc cgcgcccgtg ttcccttcaa ggtgcggcag
      361 tttcgggcag ggccgcacca aatcggcggg cggcttaacc gccgtcgtca acggcggcgt
      421 tgagttcgcg cacgccgtca gcatcaaaac aggcattgcg gtacacaggt ttttcaatga
      481 ttttaagcgt ttgcacatag cgcacccttt ctttttcctc gcgcgccgcc ttttgcgcct
      541 gatacgcggc ggacgatttg cgggcgtgtt cggcgtgttc gaccgcggc
//
LOCUS       DAWTQS010000149          580 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.149, whole genome shotgun sequence.
ACCESSION   DAWTQS010000149 DAWTQS010000000
VERSION     DAWTQS010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.149"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
BASE COUNT          145 a          164 c          123 g          148 t
ORIGIN      
        1 tcgccttgtc ctgatttttg ttaatccact ataatcagga aagacaaaaa accttccgcc
       61 gtcattcccg cgcaggcggg aatccgatcc gttgggattt tgcaacttca aatcaatccg
      121 caaactgaaa tcccgtcatt cccgcgcagt cgtgaatccg aacgcgtccg cacggaaatc
      181 cgcatcccgt cattcccacg aaagtgggaa tctagaatgt caaatctcaa gaaaccgttt
      241 tatccgataa gttcctatgc cgacggacct ggattcccgc ctgagcggga atgacggagg
      301 ggaacggcgc acggacattt gccggaatct tttatgtttc cattatcata ctcttttcga
      361 tgcgctattc caagaaaaag aaaatagctt gtgcaggctg ctttttgttt ttcagacgac
      421 ctgtaattaa cggctgctta tccaatctgc ttttcaaact acctgcaacc gcaaaatctg
      481 ctgcaacccg ctccctcccc tgtgggggag ggtttgggag agggcatttt tcaagttgcg
      541 gcaattcccc ccccgctcaa aaaattccct aaccgcccca
//
LOCUS       DAWTQS010000150          562 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 52368
            SAMN23565964-rid13876493.denovo.150, whole genome shotgun sequence.
ACCESSION   DAWTQS010000150 DAWTQS010000000
VERSION     DAWTQS010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23565964
            Sequence Read Archive: SRR17097500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241368.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241368.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:16:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,148
            CDSs (total)                      :: 2,094
            Genes (coding)                    :: 1,910
            CDSs (with protein)               :: 1,910
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 101 of 184
            Pseudo Genes (incomplete)         :: 90 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23565964-rid13876493.denovo.150"
                     /isolate="52368"
                     /isolation_source="Penis/Urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2017"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-17281"
     gene            133..>562
                     /gene="mafB"
                     /locus_tag="WNA25_002148"
     CDS             133..>562
                     /gene="mafB"
                     /locus_tag="WNA25_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HFA9824793.1"
                     /translation="MNLPIQKFMMLFAAAISLLQIPISHANGLDARLRDDMQAKHYEP
                     GGKYHLFGNGRGSVKNRVCAVQTFDATAVGPILPITHERTGFEGIIGYETHFSGHGHE
                     VHSPFDNHDSKSTSDFSGSVDGGFTVYQLHRTGSEIHPEDG"
BASE COUNT          159 a          134 c          138 g          131 t
ORIGIN      
        1 aactccgccc catccgtaga ggctgataac agtcatgagg ggtatggata cagcgatgaa
       61 gcagtgcgac aacatagaca agggcaacct tgattcacac tgccataacc gcttgctgcc
      121 aaggaaaaca aaatgaattt gcctattcaa aaattcatga tgctgtttgc agcggcaata
      181 tcgttgctgc aaatccccat tagtcatgcg aacggtttgg atgcccgttt gcgcgatgat
      241 atgcaggcaa aacactacga accgggtggc aaataccatc tgttcggtaa tggtcgcggc
      301 agtgttaaaa atcgggtttg cgccgtccaa acatttgatg caactgcggt cggccccata
      361 ctgcctatta cacacgaacg gacagggttt gaaggcatta tcggttatga aacccatttt
      421 tcaggacacg gacacgaagt acacagtccg ttcgataatc atgattcaaa aagcacttct
      481 gatttcagcg gcagcgtaga cggcggtttt accgtttacc aacttcatcg gacagggtcg
      541 gaaatccatc cggaggatgg at
//
